LOCUS       DACDLI010000001       119056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDLI010000001 DACDLI010000000
VERSION     DACDLI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 119056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 119056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..119056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.001"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(523..879)
                     /locus_tag="IV353_000001"
     CDS             complement(523..879)
                     /locus_tag="IV353_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9008284.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(893..1303)
                     /locus_tag="IV353_000002"
     CDS             complement(893..1303)
                     /locus_tag="IV353_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9008285.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1650..3836)
                     /locus_tag="IV353_000003"
     CDS             complement(1650..3836)
                     /locus_tag="IV353_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9008286.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4054..4737
                     /locus_tag="IV353_000004"
     CDS             4054..4737
                     /locus_tag="IV353_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9008287.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4818..5537)
                     /locus_tag="IV353_000005"
     CDS             complement(4818..5537)
                     /locus_tag="IV353_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9008288.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5849..6055)
                     /locus_tag="IV353_000006"
     CDS             complement(5849..6055)
                     /locus_tag="IV353_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008289.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6300..6902
                     /locus_tag="IV353_000007"
     CDS             6300..6902
                     /locus_tag="IV353_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9008290.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7043..8323
                     /locus_tag="IV353_000008"
     CDS             7043..8323
                     /locus_tag="IV353_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9008291.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8336..9064
                     /locus_tag="IV353_000009"
     CDS             8336..9064
                     /locus_tag="IV353_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9008292.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9133..10656)
                     /locus_tag="IV353_000010"
     CDS             complement(9133..10656)
                     /locus_tag="IV353_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9008293.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10908..12089
                     /locus_tag="IV353_000011"
     CDS             10908..12089
                     /locus_tag="IV353_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9008294.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12149..13777)
                     /gene="treC"
                     /locus_tag="IV353_000012"
     CDS             complement(12149..13777)
                     /gene="treC"
                     /locus_tag="IV353_000012"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9008295.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14174..16885)
                     /locus_tag="IV353_000013"
     CDS             complement(14174..16885)
                     /locus_tag="IV353_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9008296.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(16895..17596)
                     /locus_tag="IV353_000014"
     CDS             complement(16895..17596)
                     /locus_tag="IV353_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008297.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17782..18381
                     /locus_tag="IV353_000015"
     CDS             17782..18381
                     /locus_tag="IV353_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9008298.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18462..19475
                     /locus_tag="IV353_000016"
     CDS             18462..19475
                     /locus_tag="IV353_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9008299.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19583..20500
                     /gene="rbsK"
                     /locus_tag="IV353_000017"
     CDS             19583..20500
                     /gene="rbsK"
                     /locus_tag="IV353_000017"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9008300.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20528..20923
                     /gene="rbsD"
                     /locus_tag="IV353_000018"
     CDS             20528..20923
                     /gene="rbsD"
                     /locus_tag="IV353_000018"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9008301.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            20923..22296
                     /locus_tag="IV353_000019"
     CDS             20923..22296
                     /locus_tag="IV353_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9008302.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22653..24797
                     /locus_tag="IV353_000020"
     CDS             22653..24797
                     /locus_tag="IV353_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9008303.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSIEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24849..25889)
                     /locus_tag="IV353_000021"
     CDS             complement(24849..25889)
                     /locus_tag="IV353_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9008304.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26166..27968)
                     /locus_tag="IV353_000022"
     CDS             complement(26166..27968)
                     /locus_tag="IV353_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9008305.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(27982..28734)
                     /locus_tag="IV353_000023"
     CDS             complement(27982..28734)
                     /locus_tag="IV353_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008306.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28743..29105)
                     /locus_tag="IV353_000024"
     CDS             complement(28743..29105)
                     /locus_tag="IV353_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9008307.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29281..30153)
                     /locus_tag="IV353_000025"
     CDS             complement(29281..30153)
                     /locus_tag="IV353_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9008308.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30179..30691)
                     /locus_tag="IV353_000026"
     CDS             complement(30179..30691)
                     /locus_tag="IV353_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9008309.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(30829..31659)
                     /locus_tag="IV353_000027"
     CDS             complement(30829..31659)
                     /locus_tag="IV353_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9008310.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(31675..32283)
                     /locus_tag="IV353_000028"
     CDS             complement(31675..32283)
                     /locus_tag="IV353_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9008311.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32276..32593)
                     /locus_tag="IV353_000029"
     CDS             complement(32276..32593)
                     /locus_tag="IV353_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9008312.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32749..33477)
                     /locus_tag="IV353_000030"
     CDS             complement(32749..33477)
                     /locus_tag="IV353_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9008313.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33636..34517
                     /locus_tag="IV353_000031"
     CDS             33636..34517
                     /locus_tag="IV353_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9008314.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34754..35797)
                     /locus_tag="IV353_000032"
     CDS             complement(34754..35797)
                     /locus_tag="IV353_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9008315.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35800..36468)
                     /locus_tag="IV353_000033"
     CDS             complement(35800..36468)
                     /locus_tag="IV353_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9008316.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36610..38946
                     /locus_tag="IV353_000034"
     CDS             36610..38946
                     /locus_tag="IV353_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9008317.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKFSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39061..39372)
                     /locus_tag="IV353_000035"
     CDS             complement(39061..39372)
                     /locus_tag="IV353_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008318.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39553..41028
                     /locus_tag="IV353_000036"
     CDS             39553..41028
                     /locus_tag="IV353_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9008319.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41075..41659
                     /locus_tag="IV353_000037"
     CDS             41075..41659
                     /locus_tag="IV353_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9008320.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            41661..42080
                     /locus_tag="IV353_000038"
     CDS             41661..42080
                     /locus_tag="IV353_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9008321.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(42361..42645)
                     /locus_tag="IV353_000039"
     CDS             complement(42361..42645)
                     /locus_tag="IV353_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9008322.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(42997..43707)
                     /locus_tag="IV353_000040"
     CDS             complement(42997..43707)
                     /locus_tag="IV353_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9008323.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43704..45176)
                     /locus_tag="IV353_000041"
     CDS             complement(43704..45176)
                     /locus_tag="IV353_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9008324.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45314..45787
                     /locus_tag="IV353_000042"
     CDS             45314..45787
                     /locus_tag="IV353_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9008325.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(45940..46491)
                     /locus_tag="IV353_000043"
     CDS             complement(45940..46491)
                     /locus_tag="IV353_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9008326.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46596..47126
                     /locus_tag="IV353_000044"
     CDS             46596..47126
                     /locus_tag="IV353_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9008327.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47609..48052)
                     /locus_tag="IV353_000045"
     CDS             complement(47609..48052)
                     /locus_tag="IV353_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9008328.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48068..49228)
                     /locus_tag="IV353_000046"
     CDS             complement(48068..49228)
                     /locus_tag="IV353_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9008329.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49735..51435
                     /locus_tag="IV353_000047"
     CDS             49735..51435
                     /locus_tag="IV353_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008330.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51537..52370)
                     /locus_tag="IV353_000048"
     CDS             complement(51537..52370)
                     /locus_tag="IV353_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9008331.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52674..54839)
                     /locus_tag="IV353_000049"
     CDS             complement(52674..54839)
                     /locus_tag="IV353_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9008332.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55099..57393
                     /locus_tag="IV353_000050"
     CDS             55099..57393
                     /locus_tag="IV353_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9008333.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57386..58051
                     /gene="pgmB"
                     /locus_tag="IV353_000051"
     CDS             57386..58051
                     /gene="pgmB"
                     /locus_tag="IV353_000051"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9008334.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58044..59051
                     /locus_tag="IV353_000052"
     CDS             58044..59051
                     /locus_tag="IV353_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9008335.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59073..60092
                     /locus_tag="IV353_000053"
     CDS             59073..60092
                     /locus_tag="IV353_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9008336.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60373..61740)
                     /gene="nhaC"
                     /locus_tag="IV353_000054"
     CDS             complement(60373..61740)
                     /gene="nhaC"
                     /locus_tag="IV353_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9008337.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61850..63271)
                     /locus_tag="IV353_000055"
     CDS             complement(61850..63271)
                     /locus_tag="IV353_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9008338.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63514..65391)
                     /gene="tdc"
                     /locus_tag="IV353_000056"
     CDS             complement(63514..65391)
                     /gene="tdc"
                     /locus_tag="IV353_000056"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9008339.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65682..66938)
                     /locus_tag="IV353_000057"
     CDS             complement(65682..66938)
                     /locus_tag="IV353_000057"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9008340.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            67518..68819
                     /locus_tag="IV353_000058"
     CDS             67518..68819
                     /locus_tag="IV353_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9008341.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68888..69487)
                     /locus_tag="IV353_000059"
     CDS             complement(68888..69487)
                     /locus_tag="IV353_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9008342.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            69741..70622
                     /locus_tag="IV353_000060"
     CDS             69741..70622
                     /locus_tag="IV353_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008343.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            70635..72245
                     /locus_tag="IV353_000061"
     CDS             70635..72245
                     /locus_tag="IV353_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9008344.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(72322..72981)
                     /locus_tag="IV353_000062"
     CDS             complement(72322..72981)
                     /locus_tag="IV353_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9008345.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(73264..73929)
                     /locus_tag="IV353_000063"
     CDS             complement(73264..73929)
                     /locus_tag="IV353_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008346.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(74076..74681)
                     /gene="dhaL"
                     /locus_tag="IV353_000064"
     CDS             complement(74076..74681)
                     /gene="dhaL"
                     /locus_tag="IV353_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9008347.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(74709..75692)
                     /gene="dhaK"
                     /locus_tag="IV353_000065"
     CDS             complement(74709..75692)
                     /gene="dhaK"
                     /locus_tag="IV353_000065"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9008348.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(75694..76089)
                     /gene="dhaM"
                     /locus_tag="IV353_000066"
     CDS             complement(75694..76089)
                     /gene="dhaM"
                     /locus_tag="IV353_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9008349.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            76243..76563
                     /locus_tag="IV353_000067"
     CDS             76243..76563
                     /locus_tag="IV353_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9008350.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(76675..77544)
                     /locus_tag="IV353_000068"
     CDS             complement(76675..77544)
                     /locus_tag="IV353_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9008351.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(77640..78914)
                     /locus_tag="IV353_000069"
     CDS             complement(77640..78914)
                     /locus_tag="IV353_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9008352.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(78940..79449)
                     /locus_tag="IV353_000070"
     CDS             complement(78940..79449)
                     /locus_tag="IV353_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008353.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(79624..81759)
                     /locus_tag="IV353_000071"
     CDS             complement(79624..81759)
                     /locus_tag="IV353_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9008354.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(81842..83497)
                     /locus_tag="IV353_000072"
     CDS             complement(81842..83497)
                     /locus_tag="IV353_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9008355.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(83499..83813)
                     /locus_tag="IV353_000073"
     CDS             complement(83499..83813)
                     /locus_tag="IV353_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9008356.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            83983..84243
                     /locus_tag="IV353_000074"
     CDS             83983..84243
                     /locus_tag="IV353_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9008357.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            84257..84571
                     /locus_tag="IV353_000075"
     CDS             84257..84571
                     /locus_tag="IV353_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9008358.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(85301..85855)
                     /locus_tag="IV353_000076"
     CDS             complement(85301..85855)
                     /locus_tag="IV353_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9008359.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(85981..87909)
                     /locus_tag="IV353_000077"
     CDS             complement(85981..87909)
                     /locus_tag="IV353_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9008360.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(88215..88931)
                     /locus_tag="IV353_000078"
     CDS             complement(88215..88931)
                     /locus_tag="IV353_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9008361.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(88932..90755)
                     /gene="glpO"
                     /locus_tag="IV353_000079"
     CDS             complement(88932..90755)
                     /gene="glpO"
                     /locus_tag="IV353_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9008362.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(90769..92265)
                     /gene="glpK"
                     /locus_tag="IV353_000080"
     CDS             complement(90769..92265)
                     /gene="glpK"
                     /locus_tag="IV353_000080"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9008363.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(92401..93861)
                     /locus_tag="IV353_000081"
     CDS             complement(92401..93861)
                     /locus_tag="IV353_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9008364.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(93851..95146)
                     /locus_tag="IV353_000082"
     CDS             complement(93851..95146)
                     /locus_tag="IV353_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9008365.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(95478..96155)
                     /locus_tag="IV353_000083"
     CDS             complement(95478..96155)
                     /locus_tag="IV353_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9008366.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(96185..97276)
                     /locus_tag="IV353_000084"
     CDS             complement(96185..97276)
                     /locus_tag="IV353_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9008367.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(97377..97811)
                     /locus_tag="IV353_000085"
     CDS             complement(97377..97811)
                     /locus_tag="IV353_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9008368.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(98395..99852)
                     /gene="ascB"
                     /locus_tag="IV353_000086"
     CDS             complement(98395..99852)
                     /gene="ascB"
                     /locus_tag="IV353_000086"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9008369.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(99849..101744)
                     /locus_tag="IV353_000087"
     CDS             complement(99849..101744)
                     /locus_tag="IV353_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9008370.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(101802..103577)
                     /locus_tag="IV353_000088"
     CDS             complement(101802..103577)
                     /locus_tag="IV353_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9008371.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(103627..105123)
                     /locus_tag="IV353_000089"
     CDS             complement(103627..105123)
                     /locus_tag="IV353_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9008372.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(105317..106939)
                     /locus_tag="IV353_000090"
     CDS             complement(105317..106939)
                     /locus_tag="IV353_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9008373.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(107388..108701)
                     /locus_tag="IV353_000091"
     CDS             complement(107388..108701)
                     /locus_tag="IV353_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9008374.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(108946..109170)
                     /locus_tag="IV353_000092"
     CDS             complement(108946..109170)
                     /locus_tag="IV353_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008375.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(109190..109558)
                     /locus_tag="IV353_000093"
     CDS             complement(109190..109558)
                     /locus_tag="IV353_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9008376.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(109755..110465)
                     /locus_tag="IV353_000094"
     CDS             complement(109755..110465)
                     /locus_tag="IV353_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008377.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(110483..110980)
                     /locus_tag="IV353_000095"
     CDS             complement(110483..110980)
                     /locus_tag="IV353_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9008378.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(111017..111469)
                     /locus_tag="IV353_000096"
     CDS             complement(111017..111469)
                     /locus_tag="IV353_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9008379.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(111604..112209)
                     /locus_tag="IV353_000097"
     CDS             complement(111604..112209)
                     /locus_tag="IV353_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9008380.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(112659..113279)
                     /locus_tag="IV353_000098"
     CDS             complement(112659..113279)
                     /locus_tag="IV353_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9008381.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(113281..114150)
                     /locus_tag="IV353_000099"
     CDS             complement(113281..114150)
                     /locus_tag="IV353_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9008382.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(114173..114580)
                     /locus_tag="IV353_000100"
     CDS             complement(114173..114580)
                     /locus_tag="IV353_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9008383.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(114599..115888)
                     /locus_tag="IV353_000101"
     CDS             complement(114599..115888)
                     /locus_tag="IV353_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9008384.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(115915..116217)
                     /locus_tag="IV353_000102"
     CDS             complement(115915..116217)
                     /locus_tag="IV353_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9008385.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(116242..118335)
                     /locus_tag="IV353_000103"
     CDS             complement(116242..118335)
                     /locus_tag="IV353_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9008386.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNCVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(118824..>119056)
                     /locus_tag="IV353_000104"
     CDS             complement(118824..>119056)
                     /locus_tag="IV353_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008387.1"
                     /translation="KKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSK
                     TQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
BASE COUNT        34793 a        24579 c        20786 g        38898 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcag
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacaac tcagttatct tatcatgtca
      241 tttctaacat gtcaacaact attttatatg ttttttaaaa tactatttta ttgctttgct
      301 acttaaaaca acagaaatga atatatcatg taaatttaca catgtcaata tatttttaac
      361 tctatatccc cacctagatt tgaattttga ccacataaat tgagcatgtc tctatagtag
      421 aaaaataaag tcaaaaaaag aacacataac acgcttgatg ctaagtgttc gactgttaca
      481 actgtatgaa atgctcagtg acttgattaa aacagttaaa gtctatttat ttaagataag
      541 ctgttccatc aactgctcat tgtatccaat aaatagaact tcatcgataa taatcaaagg
      601 acgtctgagg agattggtat gttgtgtgca taaaaaaatc agttgttttt ttcctacaca
      661 ggaaaggtca atcgcaatgt tgtgaaaagc ctcagaccaa gtcgcaaata gctgttctgc
      721 ttgcacttct tcaaaactgc ataattcttc tgcttcttct gtacttagat cgcgttcttc
      781 taagtcaaca aattgataag gcaattgatg gtgttctaac cattgtttcg ccttcagact
      841 tgttacacag tgcgctgatc catagacttt cacttccacg aaggctccct ccctatgaat
      901 ttaagatgtt attgttttgt aaatagatca gatttctggc aacacatcga atttcacgag
      961 ggacaaatga ccggatctca tcttcattaa atccgattag tattcttttt tcatcaatca
     1021 aaataggtcg tctcaataat gaaggatatt ctttgaataa gacgatcatt tgctttaaag
     1081 ttaattcgtt aaaatctatg cccagctttt cgtaaatttt cgaccgagtg gaaataatat
     1141 cttgaactcc tccttcagtc gctgccaaga tctttttgat ctcactttct gttaacggtt
     1201 cggcaaaaat attcttctct tcaaattcaa gttgattcgc ttgcaaccat gctcttgctt
     1261 ttctacagga agtacagcta ggcgaagtat atacttttat cattttctca ctcattttta
     1321 attataatcc aattctacag taatttcttt taaaatacca attattagct aataaaatat
     1381 aaaaaaaaat caaaataact gattatttac gtttgaataa tgagaatttt tctatatatt
     1441 ctctattttg ataatagtat tctcattttc aaaataaata agggaggtgt gacaaaattc
     1501 ttgtcacacc tcccttttcc gaataaactg tgttccagaa atagcttctt cgaaaataag
     1561 ctgaaatttc acaaaaattt gagaagcaat tttcgtacat tccttcttct ttcttgaaac
     1621 tgaccacttc tgtcacaccc tctcttctat cactttcctt ttttcacacg actcccggtt
     1681 tctttgataa atctcgatac tttcaatttt cttgaatttt tcatttgagg tacacataga
     1741 tacagacgat ttcttgctct agtcatcgca acataaaata gtcttcgttc ttcttctatc
     1801 gtcgtcactg gttctgcttt tatttcatta aagtcgatta ctttcttttc tgtcgctccg
     1861 ttataaggca aacttccttc gttacaacca atcaaaaaga catttcggta actaagacct
     1921 ttacttttgt gaatgctctg gatacatagt gcatcaagtt ttccttgtct gtaagcaatc
     1981 aagcgggttt ttaattcttt agctgtcgct tgggtttctt gcgataaggc ttggagtctt
     2041 ctgaaagtag catgctgctt tacttcttct cgtatccatt cttgatcact ctggtcgatt
     2101 tccttagcta tacgtggcag atgaagatag ctttgaacaa gcgaatagat ttcttgatgc
     2161 tggtctgttt tacgcaacgc ttcctcaaac tctcggattt ctgcagattt cgattttaaa
     2221 ttggggctta tcgacaaaat ataatctgca acggctatat tttgatattt aggagaaaag
     2281 tattgatcgc attcactttt tttcaggtag cagtctgcca tcagctgccg gagcaattgc
     2341 tggtaggcct gatttttctt ttgcagattc agtgctgttt gccaatgaac aattttttga
     2401 tagaaatgtt ggaaagactt gataccaaag ataccttcat atggactatc tgcttccagt
     2461 acaaaaggaa tattcgtttt cagcagagat tggacaagct gctgcgtttg tgaagctgat
     2521 cggtataata tgattgattc cttatatttc ccattttttt gaataaatgt ctgaatttgt
     2581 ttgattagcc actgactttc tgctttagta tcagaaaaca aataggcttc cataacacct
     2641 gcttcttttt gtgctggaat catcgccttt ggtatccgat catttttatt atagctgatc
     2701 aatctatttg ctccttggac aatagctgcc cgagaacgat aattagtcaa taagaaaaga
     2761 gttttggtag gatattcttt ttcaaagttc ttgatgtatg ctggctctga tcctctgaat
     2821 ccatagatac tctgatcatc atctccaaca acaatcaact tgatcctgtt ttcctttgtc
     2881 atccgtttca cgatttccca ttgaagtgga ttagtatctt gaaattcatc aatgaataga
     2941 taatcatatt tttggccgat ataatgctgg atctcctcat tctccaaagc gcttttcaga
     3001 ttttggagta catcattgaa ctctagcaat tgctggcttc ttttttgttc ttggagttcc
     3061 gtgagcaatc catacagtcg ttctgatatc ggctgtcgtg cattcttatc aatggaatca
     3121 tatttttctt taactaactg aaccatttct tctactgaaa ggttcatatt ctgccaatag
     3181 tctaaccgag aagcaatcgt tttttctcca tattgatcaa atatataaag attatattta
     3241 cgtatcagct gttgcaaaac agcattcact ttttgtgcag tatcctttga aaagtctcct
     3301 tgtattcctt gcattgctac ttcaggaaat tttcgacaaa tttcattgta tatcctaaaa
     3361 aagatactat ggaatgttcc gatatggaca gcggtttttc tctggctgaa attatctact
     3421 cgttttttta gttcatttgc agcgttcctt gtaaaagtga tcattagtat gcgttcttcg
     3481 tctacttgtt tttgcgtcat cagataaaca gcttttgcac agattgtttg cgttttgccg
     3541 ctcccagctc ctgctatgat tcgaagatgt ttcttcatat catatgtgat agctgctttt
     3601 tgttcttgat tgaagcgatt ataggaacgt ttctccatca ttttaaagaa agagctgttt
     3661 ccttcacttt cttttacttg tacttcttcc tcaataagtc cgggtgcttc gccataagcc
     3721 gcttcatagg tagcaatgat ttcttcttgt agccgaaaga cttgttgaag ctttttatac
     3781 ttgttctccc agcgtttcaa ttctttttgt aatcggagaa tttctttttc attcaactga
     3841 ttgatttcct tcccatcttt cttctttgaa tttgtttatt tcaagttggt tatttaaagt
     3901 ttcgttgtct attttttctt tatactaaca gacctttcta aaaatgaaaa tgggaagcga
     3961 tatgatttta gttttctgaa aatttttcct ttttttcaat cgtttatgct aagataagga
     4021 agacaaaaat attcataaaa ggtgatgaaa aaaatgcaga cttcctttca cgaggggcgt
     4081 acaataggac ttttgctgac atttatcgga ggagccatgg acagttatac ttatatccaa
     4141 tatggtgctt ttgcctctgc tcaaactggc aatatcattc tatcgatcat ccaagcattt
     4201 gacggagaat ggctaagtgt tggtaaaaaa gtattgtcta cccttttctt tttcttaggg
     4261 attcttctaa caaagtatct aattgattat tttagaaaaa aagaaaaaca tttctggcgc
     4321 ttgtttgttc tatattatga agcagtgatt tttttctttg ttagtttgac acccgtccat
     4381 gagcatcccg cattagtaac gattttgatt gcttttactg catcgatcca gtgggtagct
     4441 tttgataaaa tcaacgggcg tgcctacact aatttattta caactggaaa tttaaaaggg
     4501 gtagcaacga acttatatga ttattgtatt tctaaagaaa aagcagattt tgatcgattt
     4561 ttccactatc taagtgtcgt tcttgccttt atcgcaggag ctattgtcct tgttttctgt
     4621 catcatctgt ttggcgccca ttcgattttg atcgtttcca ttttattttt gatcatggcc
     4681 ctcactcaga catttcattt atggcaattt tatcatacga atcggttcac tatttgatct
     4741 caatgaatca aaaaaactaa gcaagatgct gctcacagag aaatatctgc gagtgagcaa
     4801 tttgcttagt ttttatatta tttatttgta aagattccct ttgctttcgg gttatttgta
     4861 gtgtagcgga tcgactgggc atctgcctct tctgtcattt tctttgcccg catcttgatt
     4921 tcttgtacga tttgttctcc acatgccgga tcggctacga aatcatattt gtccccatcg
     4981 atcactattt tgggacatat atcaaactgt tcataccact cttcgtatcg tcgattgagt
     5041 tctttatagt aggaatagag ttcaggatct tgttctagct gttcatattc ccggcctctt
     5101 tttttgattc tcgctaacat agtttcaaag gatacacgaa tataaaccag tagatctggt
     5161 ttcttttgtg gcgaaatttc ttctaactcc ttcagcatcg tttctaaaag accttcatac
     5221 tgctttactt ctacttcaga aacacgccct aaatctgcat tcaaatgaaa cagcatacta
     5281 tcttcgtaga tggagcggtc tagtacgttg tcatcatggc tgaaagcatc tttgattgcc
     5341 aaaaagcgtt tgttcaaaaa gaaaatctgt aataaaaaag tatacttttc tggattagaa
     5401 taaaatagcg gcaatacttc attatcttcc acgttctcat aaaaaggttt tgtttctaaa
     5461 tcttgggcaa gcatttcagt tagtgtgctt ttccctgcac cgatcgttcc tgcaacaatg
     5521 atcacaatta tcggcatcct tcctgtaata aaatctgttc ttcattttac tacttcattt
     5581 atagaaataa aagccttgac tttatctgct ccttaaaagc tacttctcta aaagtagcca
     5641 aaagtaactc ctattcatga aaataaaggc tgtgacaaaa gtcttgtcgc agcccttttt
     5701 tcaaataaac agtattctcc tgccaacacc tcggtcacaa ggcaatattc ataaaacatg
     5761 aaaagctgtt ttttaacatt attccttgta actcagagct aaacgggaga ctcacaacct
     5821 ctttatgctc tcaacatttt atctgttttt attcttctat tgtacttgat ttgcgtacaa
     5881 ctaactgcca gtgcagatta tgagcggtat cattgtcttc tactgcgcat ttcatgacac
     5941 gtatcatcac actattttca tagatttttt ctatttttcc ttttattggt gattcaaata
     6001 aatcagattt aaatgcatat gtttcgccga cttcaaacaa ttgttcattt tccataagaa
     6061 tccctccatc accttttata aggtcagtaa ttttttaaca taaatcactc gaaaaagcac
     6121 aaacttgtca taaaaagtac ttttcgtatc aaaactgtca ctttttttgg taaggttctt
     6181 ggaaaacgaa gattttttta ggagatcatg acggttttga tgtcaaaacc actttttatg
     6241 aaacaaaaga cgttttttat tttttttagc ataaaatttg tggtggaggt acgtatgaga
     6301 tgaaagataa agttgtgaac aaattattag aagccttatt cagcggagac aaatcggaag
     6361 aagatatggt gtatgtccaa gtaaaatttg ccttagaagt tttattgaat aatcttggaa
     6421 aactagcggt cgttctagtc ttttctctgg tgacaggctc atgggcggaa acaggaatca
     6481 cctttctttc ttatatctgc atccgcagat atgcttatgg attgcattcc gactctgaat
     6541 tcgtttgtct gctgtggaca ctactttatt tatggggcgt tcccttggtg atgaaacatc
     6601 tgcaattgac tatctccttc ccaatgatgg tctttttgtt aatcagctgc tttttgttac
     6661 tgcttcgcta tggatcaaga ggtacagcaa tcaatcccat tgaacccgag aaacgtcccc
     6721 cattattaaa gaaggcgatc agcatgtttc taattttttc tctcattact ttatttttct
     6781 cagcttctta cttttctacc tatttgctac ttgggattgt tttagaaata gctacgttgc
     6841 ttcctattac aaactacttg atgaattttg gaggaatctt tcatgaaaaa aacaatcatt
     6901 aaacaattaa ctaacttatc tgactttttt gtatctggct gctgctttgg tatgagttct
     6961 gaattcaaaa tccctgaaaa attaaaaaaa taatacgcta attgtgctat aatttggtaa
     7021 aaatcaatgg gagctgtact ttatgttttt atggtccata gcgttgcaat atttgattta
     7081 cacaatttgt atcttattga ttgaccatca catccgatac aaatatctgt ttgcgggagt
     7141 cattctgatt tcttgtattt tgattggatg ggtctattcg attttaggga tttttactgc
     7201 tctaattgct ataggaatgc tgatgcttac ttttttcttc ttttcgactt cgcgtcaaca
     7261 attggtttac gcgcttcctt ttgggttatt gacgtcgctt ttaggagatc acttaacaag
     7321 catcgacgat ttttttgttt tgaaatcttc ggttattgaa ccaggagatt cattgcagta
     7381 ttttcacatt gtcttatccg cttttttatc tgctttatta gctttttgtt taaaaaaatc
     7441 gttagagact tggtttcaca ctgtggaccg ccgtatgatc ggtacgatcg ggacattgat
     7501 attatttaca tactatatca ttatttttta tacccgtttt tctggtgaaa cacctgatgt
     7561 cttagctttg aatacgagtt ttttcattct atatctgtgt gttggcctga tcatcttggt
     7621 ttattattgg aaaatctcta ataagaaatt agctgatcag ttattagagc aacaagtcag
     7681 gatgcaacag gattatatca aagatctaga aaaaaactat caagacttga gagaattcaa
     7741 acatgattac caaaatctgc ttttttctct tcacagctat atttcagaag gcgacttaga
     7801 aggattacgg tcttattatg atcaaacgat attacctaca cgccagatga tgaacctttt
     7861 tccggccaat ctaagtatgt tggataatat gaaagttcct gagatccgta gtttactgtc
     7921 attaaaactc atgatggcac aggaaaaagg gttgatcgtc cagcttcatt ttcctgagcc
     7981 aatcgaactg gatacgaaac atacggtaaa ccttgtacgt atgcttggta ttatcttgga
     8041 caatgcgatt gaaggagcta gtgctgcaaa gaagaaaaaa atcgaattga ccatactcaa
     8101 gaagaatcat tccgtgatga ttcgtgtgat caatacaaca acaaatacat taccgttaaa
     8161 ccagttgaaa caaaaagggt tctcaacaaa aacaaatcca aaaaacgaag gccttggctt
     8221 gtttatcttg gatgaattga caaagaccaa tccttatctt ttccttgaaa catcaattga
     8281 aaaccagcgt ttttctcaaa cgatttatat acaaataaaa taaaggagta atgatatgat
     8341 tcccatttat atttgtgaag acgatccaaa acaattagaa gttttatcaa cggtgatcaa
     8401 aaatagaatc atgattgaaa acttagacag ttatgtccat atttctacct ctgatccgga
     8461 agaacttctc aatgcagcca agatcagaac ttcttctttt ggtatttact ttttagatat
     8521 cgaactagaa gacagtgaaa tggatggtgt agcgatcggt aaattgatcc gtgaattaga
     8581 tcctttagga aaaataatct atgtgacttc ccatacggaa atgtcgatga aaatcttaaa
     8641 aagcaacatc gaaccgactg attatatcgt caaagaagat ctttatgatt taaaagaaaa
     8701 tgtcgagcat atcctgagca aaattttcga agattttcaa atgtcccaaa tccaaaaaga
     8761 tatcttcaaa atagagttta atgatgagat caaattttta ccgatcaaag acatccaata
     8821 tttttctact tcgctaggag cgccacataa actggaagtc catttgactc atgcccaatt
     8881 acaattctat gagaagatca aagaaatcga gaaaatgcat cggaatttta tccgttgtca
     8941 taaatcctat gttatcaata cacaaaatgt gcggtcgatt aataaaaaaa ccagagaagt
     9001 cacgttggca aacggcgaga ccatcccagc ctctatccgt ggattaaaga aattgatttc
     9061 ataaaaagaa ccgaatagtt tggaccgact aaatgaccag ccgatccaaa cccttcggtt
     9121 cttttttgcc tatcagtgca aaagcatttc ctgtgcaatt tcacgtccat ccattgtatc
     9181 tggataaaat gttggccaat tctccatttc tttcagcaat acttcttgac tttcatttcc
     9241 tgcatataaa tgaaaatgtt cggagtcagc cggtgcaatc gtgtgatcac tgaattgaat
     9301 atatctaaaa gcgccacttt ccttatcttc tgcttcgaat agatacctta cgccacggtt
     9361 tcctgcctcg taggtcaaga tttctttccc tacataacga taatttgaac gtttcgttgt
     9421 tccatcctta aacgtaaatg ccatctcttt ctcactgatc ttgattgaag caatatctgt
     9481 ttgatagccc ttcgtataat attctttata ttcttgtgct gtcttatcct gattcaattt
     9541 ttctttatat tcaaaaacag gatcaaaagt cccatcttct acatacgtat ctactgtttg
     9601 ccaagttcct gcccaatctg aaagagagcg atcttgtaca tcctcgtctt caaaataacc
     9661 gttgtagacg ctcttttctt ttttgggggt caaagtatct tctagcgggt tttctgtctc
     9721 tgtcgttttt ttcaacgctt cgaggtttgc ttccatgata gaaagatagt tttctccatt
     9781 ttctatttgt tcattcgtca atccttccaa aggattcaat acttctaaag atacacctgc
     9841 ttcgtttgct aacgttctgg caatactatc tttggcgttt tcttcaaaat aaatatagtt
     9901 gattccatgt tctttaacaa agtgtttcag ttcagcaatt ctcgccgcag acggttcttc
     9961 actagaagaa agaccagcaa tcgatacttg attcaatcca tattctaacg ctagatagtt
    10021 gaatgcagca tgttgcgtga caaaagatct ttgttttgct tgcttaaagg tttcttgata
    10081 aagctgatgg agtgcttgaa gtttctcggt ataggctgct gcattttttg tccatacttc
    10141 ctgtttttct gggtaagctt caatcagctg atcacgtatg tttgctacct gcttgattgc
    10201 taaacttgga gctagccata catgtgggtc tagctcatgt gtatggcctt cttccccgtg
    10261 ttcatggtca tgatcatgtc cttcttcact tcccggaagt aaaagcatac cttctgtcgc
    10321 ttttatcgta tggacattgc cttcttgtaa tgttttttgg attgcgggga cccatgtttc
    10381 catgttttca tcattataaa caaagatatc tgtatcttgg atctttgcca tgtctttagc
    10441 agaaggttca taatcgtggg aatcagtccc tgctggaatc aataactcta cttctccttc
    10501 atccccgacg attgcttttg taaaatcata catgggataa aaggtcgtca tcacgctcaa
    10561 tttttcttta ctgtcctcgg ctttcccact tgccccgcaa gcagttaaca tacctgctaa
    10621 aaccagcatg gccaatatac ctatcaatcg tttcatttat gacactcctc ttctcatctt
    10681 ctttcatttg aatgttccga ttcgcttttc gcaaaccgta atagttacgt tttagcaaca
    10741 ggatttaatt tatcatatgg ttaaaagaac tgtcaagcgt tttcttattt taattgatga
    10801 gaaagtttat atgaatgatt gacgatattt ttgcaatcaa cctctatcat tttcctttta
    10861 tacccgcttt tgtctataat gaagttatag ataaaggagg aaaaagagtg aaaaaagaaa
    10921 cagatacaac gaatcgtttg atccgctttt taggaggaaa aacctcttac tatgttttag
    10981 gaatagttat cttacttgcg ctgatcattt attttttcca tcagatctcg tttatattca
    11041 agcctgtcgg aatcattatc gctactatct tgcccccatt attgtttgca ctgatcttgt
    11101 attatctttt ggaaccagtc atcaaacgtt tgagcaagca cctttcgagg aacttgtcag
    11161 tcattatcgt ttatgtgatt attttatttt tattggggct tggaagcata tggcttattc
    11221 cttttcttga agaacaggca aagacattga tcagtagttt gccagatttg tttcaagatt
    11281 ttcaagagag tctccgacaa tttttggaga aaacaccttt tgccgattca tttaatcaat
    11341 tttttgcttc tattgacgac gtgacgtctg atatagctgg atttattgga aattattggg
    11401 aaagtggcgc acaacggatc agcaacattt tttcaactgt tacagctatt tttattactt
    11461 tatttactgg tccaattgtc gcttttttcc ttttgagaga tcctcaaaaa ttttatcagt
    11521 cagttttagc gattgtccca ccagcatttc gtacagattt taaaaatttg acaaagattg
    11581 ctaatcaaca attgggttca tttctaaagg ggcaaatcat tgcttcattt atccttggag
    11641 cagtctactg ggtttgtttc ttactgatcg gattagaatt tgctagtgtt ttagctattt
    11701 ctgctgggtt gttatgtatc attccatata ttgggccgtt tatcgccttc tttccaggtt
    11761 tgatcattgc attccaagac tctactttta tggctgtcaa atttgtcatt gtctggtttg
    11821 ccgtacaact tctccacggt gacttggtga ttcctcgagt aatgggtgat cgtttacaga
    11881 tccatccgat tacgatctta atcgttttac ttgtcatggg tgatctaatg ggaattgtgg
    11941 gcgttatttt tggtattcct atctatacgt tagtcaaatt actcgttact tttattttcc
    12001 gtaaattcaa acaacgctac aataaatttt atggcgataa aggagagtat gaaaactctt
    12061 cattttctga agaagattat tttaaataaa acacaaaacg tcgatcttac gagaatagct
    12121 cgtaggaacg acgttttgtg tttttgtttt aatttttcag caaagcaatt gcctggtatg
    12181 gttttagctc cagactttga tgaagaacag tatcggaata attatcgata agaatattgg
    12241 cttctgtgaa tccttctggg atggcgatcc ttgtatcttt atcatagaaa tttgttaata
    12301 caagcagctg ctgtccttca aattggcgaa gaaagccata aacttccgga tgatttgctt
    12361 cataagcatg ataatctcct tgtgcaacga ttggatatgt ttttctcagc cgaatcaatt
    12421 tcttgtaata ggacaaaata gaatcttcag aagatagttc tttttctaca ttaatctctt
    12481 ctagcttgcc tccaactttt aaccaaggtt tccctgtagt aaatcctgct tgttctgtgt
    12541 ctgaccattg catgggtgtt cgagagttat cgcgagattt tgctttgatc cgacgaaacg
    12601 cttctttttc tgtgaatcct tgttcaagga gtaacttata agcattcaaa ctttctacgt
    12661 caatatagtc ggaaatcgat gaatagtctg ggtcgatcat gccgatttct tctcccatat
    12721 aaatataggg cgtcccacgg ttcaaatgga tcatggttgc tagcatctct gctccttgtt
    12781 ttcgatactg atcgataact acaaatcgat tgagcgcacg tggctgatca tgattattaa
    12841 aaaatagagc attccagccg ttaccctcac tcatattttc tccccatgta tgaaatagtc
    12901 gctttaattc tgcaaaatca aatggaggag tagaccattt attcccattt tcataatcga
    12961 ctttcaaatg atggaaatta aagaccattg ataattcttc tctactaggt tctgtataca
    13021 agatacaatt ttcgatcgtc gtagagctca tttcaccgac tgtgatgata gtggaatccg
    13081 tcccaaacgt tgcttggttc aattctttta gatacgtatg ggtgattggt ttgtccgtat
    13141 attcaggttt gccatcattt tctttattat ttttcaattg ttcgtcttta ccgataacat
    13201 tgataacatc gaaacggaat ccttttaccc ctttttccat ccaaaaacga acaactttga
    13261 atagttcctc tcttactgct tggttccgcc agttcaagtc agcttgtgtc cgatcatata
    13321 agtgcaaata atatttcccc gtatctccaa atggcgacca agcacttcca ccaaattttg
    13381 atacccaatc cgttggctct tctcttaaaa tgaagaagtc ctgataatgc tggtcccctg
    13441 ctagtgcttt tttgaaccat tcatgttctg tcgaaacgtg attcagcacc atatcgagca
    13501 tgatttctat ccggtatttc tttgcttcgg ctattagctg ttcgaattcc tccatcgtac
    13561 caaagatagg atcgatggct gtgtaatcag agatatcata accgttgtct ttttgagggc
    13621 ttggataaat agggtttagc caaatcatgt cgatgcctag atcattcaga tacgggagtt
    13681 tttctttgat tccttttaaa tctccaattc catcaccatt actgtctttg taagatttgg
    13741 gatagatttg gtaaatgact ttgtctttaa atgtcatgga tatttacctc cattcaactg
    13801 atttattacc taccacagct tatatataca agttatcatt tattattata actgaacaga
    13861 aagcgtttgt aaaatttatc tttatacatt ctaagaggtc tgttgcccaa taaaaaacaa
    13921 gagatcagac atgtatttgt ctatcctctt gtttaaaata gcaaaatatt cttgtttctt
    13981 ctataaatgg tgttcaaaag cttttatcta cggcaatcag tcttctcaaa tgtaaaacat
    14041 gaggagctaa ttttatttga gtattctttt tgctcgatgc aggacggctt ttttacaacc
    14101 tctgattcat ggtgttcaat tagcagctga ccctcgttat tagctcaaaa cgctgatttc
    14161 acaacctctg tttttattca ttcgatttca acgcatcaat catatcgata tgctttaatt
    14221 tgaaatgggt aacaatcatc acaataaccg taaatacgat agttaatcct gctgataaga
    14281 cataagccca ccatgtgatc actaatggaa agatgacatt ctccatagac gcttgttgca
    14341 agatgaagtc agtcaatata tatcctacac caaatcctcc gatgattcct aaaagagtaa
    14401 agatgatatt ttctcgaaca atgtacatcg tgacttcttt atcaaaaaat ccaaggactt
    14461 ttattgtaga taattcgcgt acacgctcgg aaatattgat attagttaga ttatacaaga
    14521 cgataaaagc cagcagtcct gataatacga caaagattaa gacaaccgag tctaagttcg
    14581 ccattgattc ttcttgcgtt tcgatttgtt tggacatgaa agacgtattt gtcacagcgt
    14641 ctgtggcaag cagtttctcg gaaagcattt cttcttcttt tgttgtcatc ttatctgtac
    14701 gcagcaaaaa tgcattatcc atcatcgatt gatcagtaac tgaagcataa tattcagggg
    14761 acatataaat aaagctacct agatagtttt tcacgatttc ttttacttcg actttccatt
    14821 ctttttgatc ttcatcataa agcgaaatat gatctcctgt agataagtca aatagttctg
    14881 ctactttttg cgtaatagcg attcctgttt taggcaagga gcgtgggttc tcttcaagat
    14941 cagttaaatg gataaaacga tccaaatcag ctgttttact tggtacgatc actgtcagac
    15001 tttgcgaagc atgtcctttt tttctcaatt ctacggtttg agtatgaata gctaggatct
    15061 catttacttt ttcttgcttc tgcagcatat ccgctgcttt ttgacgacta tcttccgtgg
    15121 atgaatccaa tgtaacgatt gcttgatagt caatgatcgg tccaaattgt ttggctgaaa
    15181 cagaacttag agaatctttc aatcccacgc ctgcaaccat cagcccggca catccagcaa
    15241 ttccgataat cgccataagc attcttgact tatatcggaa gagatttcgg taactgattt
    15301 tttgattaaa gctaaggcgt gaccacagcg gagtgatgta ttcaagaaaa atgcgtttcc
    15361 ctggcttcgg tgctttcggt tgaagcaatg cagcaggccg ttctcttaaa tctttgaata
    15421 aaacgatcat tgcagcgcca aatgcggcaa tgaagaatgc ggcagctgct tgtaaaatcg
    15481 gcggccagtc ataaaaaata agcgcactcc caatatcata ccgctcgtta gacagctcaa
    15541 aaatgatcct tggcaaaaga ttcgttccta atatcgttcc taaaacaata cctatccctg
    15601 aggctagact tgcatagatt gcatattttt ttgcgatttc aactttcgta taacctaacg
    15661 cctttaatgt cccgatttcc ctgcggtttt cttctaccat acgtgtcatt gtcgtaaagg
    15721 tgatcaatgc ggcaataaag aaaaagaaaa caggaaaaac attggcaatc gcagcgatac
    15781 gatcagacag atcaccaaat tcttgaaaac cgacattttc tgaacgttca ttgtataggt
    15841 atgatggttt agttaaattt tcaatttttt ctgcatttcg attcaattct tcctctgcag
    15901 aggccagttc acgttctttc tcaactaatt gcctttcttg ttcagcaagt tgagcttctt
    15961 gttgaggtaa tgtttgaaca gcgattgttc gttgcgcttc aggaagctgc tggatagttg
    16021 ctgactgttg cgctaactgt tttttagcat cttctaaccg tgttcttgct tgcgttgttt
    16081 gtgctttgcc atccgctatt ttttgctgtg ccggttctaa ttcttcgttg gccgattttt
    16141 tcagctcttc taagcgttct tcgggtcgat cttgtaaata cttttctgcc ttttcttgat
    16201 tttgctccat tttatctttg taagctttgc tgtacgtatt caaattcttt acgtttgtaa
    16261 aatcaagata taagacagaa taaatatctg ttttaaaaac ggattcaggt aaatagacaa
    16321 aataatcgac gctaccattt ccgacgttcg cgtatcctct ctccaaattg ttgatgaaca
    16381 atggagaacg gacaaagccc gtgatggtat actctttttc tttcaattca tctgaatcaa
    16441 tctgataaat atcaccgatt ttataccctt catcttccgc tttttcatcc aatacaagct
    16501 ggtttggttt ttccgggagt tttccatcag ttaattgtag cttgttctgc ttgttatttt
    16561 tgttataaga aaatatttga atcacttcgt ttttgccagt attggaataa tagatctgtt
    16621 ttcctgtttc tacttttgca tctgggatct tttctgcttg agcaatatct ttttgtgtca
    16681 agccaccagt agaaacgatt tgcaaatcag aaagtcctgc tgtaccaaca tagtgatcca
    16741 tcgttttttg catgacaggt ccagcagttt tgacacctac aaataaaagt gttcctagca
    16801 tgacaataag gatgattgca atgaatcgtc ctttagattg ccgaatttcg cgaaaacctg
    16861 ctttaagata tgttcttgtt ttcataaaat ccccctacca tacaatctca tcaatggatg
    16921 aaggatgatc atttaattca acagacctta ctttagcatc attaatgtga atgactctat
    16981 cagcgatggg cgctaaagca gaattatgtg taatgatcaa aacggtattg ccgtgttgtc
    17041 tgctagcgtt ttgcaataat ttcaatactt gcttgcccgt ttcaaaatcc agcgcgcctg
    17101 tcggttcatc acataaaaga agtttgggat ttttagctag tgcacgagca attgaaaccc
    17161 tctgctgttc gcctcctgat aattgggacg gaaagttatt caatcgatga gctaggccca
    17221 cttgcctcaa tacctctaca ggatcaagcg catcaggaga aacttccgtt gccaattcca
    17281 cattttcttt tgctgttaga ttaggaacga gattgtagaa ttggaaaacg aaccctacat
    17341 ctgttcgtcg gtaagcagta agctgtttat ctgaaaattg cgcaatatct gtgtcatcaa
    17401 taatgatctg accttcatca ggtgtatcca tcccacctaa aatattaaga atcgttgact
    17461 ttcctgctcc acttggacca agtataatca ctaattcgcc tttttcaatt gaaaaagaaa
    17521 tatcgtcatt tgctgtaatg gtcgtttctc ccattttgta tcgtttgtat tcatttttta
    17581 cttcgacata cttcactctg attcctccat tttcacgata tttctcgcta tatctatgca
    17641 tcgaatgcgt tttctcacac ttatttattt taccactgaa tttctctaga ctaaagaaag
    17701 gaatccttta atttagttga caaataaacg atttatgatt tacttacata aaaaacattg
    17761 ttatttgaaa gaaggaacat catgaatgat ttaaaggaaa atatgatttt tgatttatca
    17821 aaaactgctt ttgtcgcgat cgacatgcaa aaagagatcg taaataacgg tacactctct
    17881 ccacacactg cagcatctat tctgacaaac aatgatttgt tagtaagaac attgaaacgt
    17941 accgctgcct tgaaagtttt agtgaacgta gacatctcca ctttccctta tttgagtcaa
    18001 caaactgata tgggtggaat gactgcttct gtacctccag aattcactga tttacttttg
    18061 aaggattctt tgaaagatac agataatatg ttgactatca aaaaatacaa tccaagtgct
    18121 ttttttggta cttctcttga tcttcaactt cgccggcgtg gaatcgaaac tatcatttta
    18181 agcggtgtag cgacaacaaa cggtgtatat gctactgcat tagatgcctt tcaacacggg
    18241 taccatatcg tgcttgctga agatgcttgc agtgatcgtg acaaagaatc ccatcaattg
    18301 ttcataaaaa agatatttcc gaaaactgca cgagtacgct caacgaaaca aataatcgaa
    18361 gctatccaac aaagcaaata agtactggca cacgtgattt tcatttactt ctttttgatt
    18421 tcatgggaaa ataaccacag gatttagata ggagtatgaa catggtgaaa aaaaagatca
    18481 caatcaaaga agtcgctaaa ctttccggcg tttcaatcac aactgtctcc ttgatattaa
    18541 atggaaatac ggagaaattc agtccgaaaa ctgttcagaa agttttagct gccaaagaag
    18601 aattaggcta tcagcctgac tattttgcta gacaaatgat taccaaggaa acgaaaacaa
    18661 ttggtgtact cgttcctgat atcacgaatc cattcttcag tacactgatg cgaggaattg
    18721 aggatatctt gtataagcag aatttcgtca cgatattatg taatgctgat tctgatcatc
    18781 aaaaagaaat cgaatatcta gccgagctca cacgtcgagg tgtggatgga ttcattatcg
    18841 cgacttctgc tgtctctaca gatgcaatca acgaaaattt gaaaaaacaa ggccggccct
    18901 tcattgtttt ggatcagaaa aaatctgaag gatttagtga tgccgtacga acagacgatt
    18961 ttcgcggtgg ttacctagcg ggcatgcact tgcttagtct aggacatcaa acgatcgccc
    19021 ttgtctatcc tgaaaatcca ccggaaaatg tccacgctcg gattgagggg tttaaatctg
    19081 ctcttgatgt ttaccaaatc cctcacgacc aactgatcct cttacctaca cagttctcaa
    19141 aacaaggtgg ctaccagatc acagcagaac ttcttgattc tgctgcaact ggtgtgtttg
    19201 ctttaaatga tgaattagct tttggattat atcgcggatt agaggaagct ggaaaaagca
    19261 tccctgaaga ctacagcatt attggctatg ataatataga tatgtgcgag tatatcaagc
    19321 cgaaactgac aacgatcgca caaccaatct tcgaattggg acaaacatct gccaagttat
    19381 tgttagatcg gatccaattc ccagaaaaag aatgggaaga aaaaagatta cctgtacgat
    19441 tagaaaaaag attttcaact gcacccttga aatagctttc aaggtgtgct atatttttat
    19501 cgaactatta aaacgtttta ctaaaacgtt ttaaaaaata attttcccat caaaataaat
    19561 gagatagttg gaggtttgta aaatgaacac ggttacagta attggaagta ttaatatgga
    19621 cactaccctt cgtttaacca acatgcccaa gccaggcgaa acgatgcatg cgcatgagat
    19681 ttttcacgct ggcggaggta aaggtgccaa ccaagcagtg gcagccaaac gttcaggcgc
    19741 acggacatct tttattggag gcgttggggc tgacagcgaa ggacaacagt tattagatct
    19801 actgacaaaa gaaaacatcg atacatctgg gatagctgaa atccaaggcg ccacaaccgg
    19861 acaagcaatg attatggtag acgcagcagg agaaaatagt atcttgatcc atgcaggggc
    19921 aaataatgct ttccatgaac aagaagtcct aaaaaacaag caactgatta caaacagtga
    19981 ctttattatt gctcagtttg aaagtagctt agatgcgacg atcctagctt tttctattgc
    20041 aaaagacgca ggaaaaacaa cgatcctgaa tcctgcacca gctcgtgaaa cgattcctac
    20101 tgaactactt gaaaagacag atatcattat accaaatgaa acagaaacag aaatcattac
    20161 aggaatcaga gtgacagatc ataacagttt ggtcgcagca gccgaaaaac tccatgaatt
    20221 aggtattgga acagtaatca ttacactagg cagtgccggc gctttctatc atacagaaaa
    20281 agaacacggt atcgtccctg cttttaaagt ggatgccgtt gacacaacag ccgcaggtga
    20341 tacatttatt ggcgcattaa gcagcacact tcagccagac cttagtaact tgaaagaagc
    20401 gatcctttat ggaaatctag cctcttctgt tgctgtgcag tcttatggtg cgcaaccttc
    20461 tatcccttac cgcgaagctt tagaacaatc agcagtctaa aattaacaat gagaggaaga
    20521 aacaaacatg aaaaaaggaa aagtaatcaa ttcagatatt tcacgagtaa tcgcacaaat
    20581 ggggcacttt gataaattaa gtatcgggga tgcaggcatg cctgtcccct ttggtactga
    20641 aaaaatagat ttggctgtct caaacggtct tccaagtttt ttatctgttt tagaaaacgt
    20701 tttagaagaa cttgaggtac aatcgattga attggcggaa gaaataaaga caatgaaccc
    20761 ggaaatattg gaaaaaatca aattattgtt accagataca ccgattagct ttatcccaca
    20821 tgaagaaatg aaaaaagagt tgaataactg tcatgcattt attcgtacag gcgaaatgac
    20881 accttacgcc aatattatat tagtgagtgg agtggtcttc taatggaaac gacaatgaat
    20941 cctgaacaga tggtactgtc cgagcagatt catatgctga ttgaacagtt gggaggaaaa
    21001 gacaatattg attctgtcct tccatgtgcg actcggcttc gtatcaaatt gaaaaaacag
    21061 ccagtagcca atgcagaaaa attaaaacag caaatccctt ccattaagta tttcatttta
    21121 ctcgatagat atgcagaaat aatcatggat cagggattgg ctcctgctta caaggaattg
    21181 acgcgtcaaa tcgatcattc agctccgaaa gaaaagctaa atccgatcga aaaactattt
    21241 ggtttgatat cggaaatctt tacgccgatc attccgctgt ttgccggttc tggtatatta
    21301 aaaggtctgg ttgttttagc gactacgatt ggctggctta gtgaaacaag cggaacttat
    21361 catattttgt ctgctgcgtc gaatgccgtc ttttattttc tgccaatctt tcttgctttt
    21421 tctgctgcca aaacgtttaa agtgaatgga tatattgcgg ctgtcattgc tgcagcactg
    21481 attgaaccga atctgacctc tttactttca gacagtggca aagcagctac tgattttctg
    21541 gggattcctg tagtactgat gcagtatacg tctactgtca tgccggcgat tttaggtatt
    21601 ttcttttatt cttttgttga aaaattcctc aaaaaatata ttcgcgaaaa tatgcaatta
    21661 gtatttgtac ctttactttc attagttatc acggttcctt tgacgttaat ggtattcggg
    21721 cctgcgggtg tttaccttgg cgaaggttta gctgccgcaa ttacttggat gatggatatt
    21781 aacggaccga tcagtggcgc aatcatcgct ggtggttgga atattttggt aatatttggt
    21841 atccaatggg ctgtaaaccc tgtcatgatc agtaatatct ctgcttatgg gttcgaccga
    21901 atcgttccgt tgacaggagc agccaatttt ggaatggctg gtgcagcttt aggtgtattt
    21961 ctgcgaagta aacgttcaaa aacccgttcg atctccggtt ctgcttttgc ttcgatttta
    22021 cttgcgggag tgaccgaacc aactgtttac ggaattgcga tcccattgaa aaaaccattt
    22081 gttgctgctt gcattggtgc agctgctggt ggagctgtaa tgggatttgc ccaagtgaaa
    22141 gcgatcgctt ttgtatttgg tagtctgacc acattgcctg cattcatttc cggtactttt
    22201 ttctggtact tagcaggact ggctgttagt ctagtcgtag caatgatcac tactctcgtt
    22261 agcgggtttg atgaagacct aatgtcttat gaatagttaa tgaaaaagac tgaagtatga
    22321 gatgtttatc acatatttca gtctttttct ttttaggatc attattttta taaatttttg
    22381 tgatactttc caaactttgg tataatttac atatgcataa ccatttttat ccccacttgt
    22441 ttatccccaa acaaaggtgg tctatgcaat aaaatgaacg tcaaagaaaa acgaatgaat
    22501 gctcgttgct tatttacaaa ttgttttatt tagaatacac agacagattt atgaaaagaa
    22561 aaagcttttc gacacgatta tctgttccaa aaaatgtcta atgaatgctg aataagacag
    22621 aaaatcaatg aaaggaaaag gactagttta ttatgaaaaa taaaaaaacg atacgtttgc
    22681 tagctgctac ttggctgatt gctccgtctg ttttgtcgac caataccgta tttgccgtag
    22741 ataatcaaat agctgatcaa acagtcacaa cagatacgac tacatccagc tcagctacta
    22801 gtgaaacaac ggattctttt acagaagacc caactggaac aactaactca acaacaggat
    22861 cgaccgagcc aactgaaatt gatccggctg atccatcaga aaatgaaaag ccagccgaga
    22921 catattccct tacaattgct ccttctttga ttggtaagat ccaactgact gcttcgacag
    22981 cggattcccc tgaacagcaa cctttggata ttacggaaag tggaacggta gaaggtttat
    23041 tagcggaaac gaaggtaact tacactatta cgccagctga aggtgaacaa attgcttcag
    23101 tcgctgttaa tggtgccaaa gaagtaaatt atgatgcaaa gacctttaca gtaggtacga
    23161 acaatttatc tattactgca gaatttaaag ctggaccgga tacttcaata gagaaaccaa
    23221 ctgatcctag tgaagaagtc cctagtacac cagatccgga aacgccaaac aaaccagatg
    23281 tgggtaataa caacaataat aatggttcaa ataattcaaa caataataac aataattcga
    23341 acagcgggaa ttcttcgaat caaacaaacc catctggatc gaataacaat ggaaatactc
    23401 agagagaacc aaaccatcca aacaatggtt catcgaatgg cggaacgaac aatgcttcat
    23461 ctcgtccaaa tacagcaaat caaagcggta tagagaatcc atcttctgct tcttctgatt
    23521 ttgttgtaaa atcaccaatt gatgctgtat tgcctacaaa tacaacgaat gtccaacaag
    23581 caatcgttcg agaagcattc aagcatcttg gaaaaccata tgtatgggga gcaaaaggtc
    23641 caaatacttt tgactgttct ggtttgactt attacgtata tatgaaagca accggtcatt
    23701 atatcggtgg ctggacaggc gaacagcaat atgcaggtac gcagattcca gtaagtcagg
    23761 cccaacctgg cgatctcgtc ttctggggac cttcttccgg tgttactcac catgtaggta
    23821 tctatattgg gaacggacaa tttatccatg cgcctcaacc aggagataaa gttcgagtaa
    23881 cttctatttc agatttcacg cctgattttg ctgtccgagt aaacttagca ggtttacctg
    23941 ctgcttcagg ttcgctgaca ggcggctcat ctattttaga tggattagat ggctcgttcc
    24001 atttcagtca aaaccaaaca actgatcagt tcttgaaaaa gattgcagat gatgcacagg
    24061 aaatcgggca aaaagaagga atctacgcat cagtcatgat ggctcaagct atccttgaaa
    24121 gtggatctgg aaacagctta ttatcaagcg aaccaaatca taaccttttt ggtatcaaag
    24181 gatcatataa aggaagcagc gtgaccttca atacacttga acaagatagt tccggacaaa
    24241 gctaccaaat ccgcgcacaa ttccgtaaat atccttctta caaggaaagt ttggaagact
    24301 atgccgactt gatcaaaaat ggactgactg gcaatcctga tttctacaaa ccaacttgga
    24361 agtcggaaac aaaagactac aaagaagcaa ccaaatattt ggaaggacgt tatgctacag
    24421 atcgccaata ttcacaaaaa ttgaacgcga tcatcgaagc gtacgacctg acaaaatatg
    24481 atgaaccgaa aaaagaagca gcaacaactg aagaagcaaa cgctgaaatc ctcaatatgt
    24541 ctaagcgatt cgatgtgcca atcaaatgga caacagatgg cgtgccaatc acaggattag
    24601 aattttcaac gataaaacgc gtcaacaacg cttcttccat cttccgtgtc gcttcaatct
    24661 gggatttgtg gaacaatttt acggcaagac agataccaga aacaactact cgtacagtta
    24721 aagctgcaac tgttcctgta ctaaccttgt taagtttaga caaatcattc tggcttgatc
    24781 tgcctttaaa gaaatagaat aaattaaaaa ggggatgtga cactgtcaca ctcccttttt
    24841 aatttattct atttctttat atttattttg tattccgtta caaactcgcg tacttggtta
    24901 ggttccagag tgacatctcc aaaactttca agtcgctccg ctcctggcag ttcttgagtc
    24961 tcaaatgtga taccgccatg atttactaat ttattcccgc gcatttccgg tccatcttct
    25021 ccaaaatttg ctgtaaaaat cacgattcct gaagcatctg tttttacttg gatcgatact
    25081 ttttcatctg ggcttgtcaa ttccgcagca tttagtaaac cagattgttt tttcaacaaa
    25141 aatggatgat cgatgccgcc cactatcatt gattggcgaa aatcactggc aaagacctca
    25201 cctaattttt taggacgttg gaagtcaaat gctgtgccac ttactgaaac tacttctcct
    25261 gtaggcgttg tatctttatt gagcgtagca aataatcggc tattcaacca aagtatatgt
    25321 tcgtcgattg actgtgctgg atcaccggtt aaattgaaat agacatgatt tgtaggattg
    25381 aatactgttt tgcggtcact gatcccttgg accgtcactc gccatgtatc atcgttcgtc
    25441 agcgtatacc gtacttctac atctaaagtt ccaggaaatc cgttttccat atctgggcta
    25501 gtctgataga aaatgaccga tgcttcgttt tcaccattga tgactgtata atgccactgc
    25561 ttggcttcaa agctaggtgc accaccatgt aaacaatgac cagtgtccgg gtctgttttt
    25621 acttcgaacg tttcatcatc taatttgaat gtgccattcg taatccgtcc agccatacgt
    25681 ccgattgaag caccgatata agggtctttt tctagatatt cttttgcttc atcaaaacct
    25741 aagatcaact ctcgtttttc attatcgatt attgtttgaa aatgaacaac ccgtgcccca
    25801 agatcactga cagacaaaga tgtcccattt tgattcgtga tcgtaatcaa gtgatagttt
    25861 tcgccaaaat tttgttcatt gatttccatg ttccttgcac cctcttttct ttcatttgca
    25921 ctgattccag tatacatgga aaccgttttc ttaacagctt attccgtgag taacgagaaa
    25981 aaatttacta tattctaata aaagaccgtg aaaaaaaggt tgtgacaaaa atattgtcac
    26041 aacctctttt tccgaacaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    26101 aaacgaaaaa tatgaggagc tatttttgct tggctgttct tattacttga tgcaggacag
    26161 ctttttcaca atctcttttt caatacgatt ccagtatata accatgtcgt cagtacatag
    26221 tagatgaaat acaatacgaa gaagatcgta aacggaaccc aaatatcttt gtatggattt
    26281 tgcatgaata gaccaaacat ttgtaatccg aataagacat ggactacacc taagattcct
    26341 ggtgctaaga ataacacgcc tacttctcta cggatcgatt gtttaagcaa cccgcgtcgt
    26401 gttcctatct tttcaagcat ttcataacga gcaatgtcac tcttgcttcc tgacaagatc
    26461 ttgaacatca aacagctagc aagcatggtc aagaaagcta aacctaggaa gaatcccata
    26521 aattcaaaac cagagaatag accattatac acttcataca tttcatattt ctggttgctg
    26581 tactctactt tttgcaaaga tggattgttc attttatttt gagcaacaag tgtttcgatt
    26641 ggtttcaggt ttgcttggaa gtcttttacc tgaactagct gtaaagtgga ttgtggcaag
    26701 ttcagttgat caaattctga ttgactgact accttgattt ctttttcttg ctgttccggc
    26761 aattcatagc ttcgaagttc ctcttgtaca gtcagttcat tcattttttt agcaggaact
    26821 ttttctttcg tgatcttcgg cggtgttcca tcttcatgtt ttatcatgat caatggttga
    26881 gccgcaaatt cacttgcatt atagtaaatc gttcccgcat cttctttttg agtataagaa
    26941 gcagaaagtg ttggtgaaag ttctttgatt ttttgctgat cttctgcccg aggattattc
    27001 atcactagat catatccaga attgttttct gtcaagtctg caatttcatt tttaaaacct
    27061 aatcctaccg tcaaagcgcc cagtgccaag gcaaacagca tagcgaccat tgagagcatt
    27121 tgtgtatatt cacgaatacg aaagtttaac tgtgacaatg tgaaattgtt caattttttc
    27181 aatgaaatct gttctgattt ctttagtaat gataaaaaga aaatgatcac agaatggaaa
    27241 atgaaataag ttcctaatat gatcgtaact aaggctaccc ctataccaaa gacttgatag
    27301 gtccccacgt gtttcatcat atagtaaccg cagcctagaa aagcaattcc cagtactact
    27361 tcaaggaaaa acaagaattt atgctgcttg attcttgttg gtgtctgatc cgcacgaatc
    27421 aatgctaaaa tcggtttttt gacaatcgaa aaagcattga caacagcagc taataaaaac
    27481 aaaacagcaa aaaacaaaac agtgatcaac aacccgtgaa tactaaaagg tgtaaaatgc
    27541 gaaatattga gatctaactg atccactaat aatcggtgga cgatacttgt caatcctaca
    27601 ccgacagctg aacccactaa agtagctgcg atccctacta agaatgtttc agaaaaaatc
    27661 atctgtgcga tttttctgcc ttttgctccc agcatcataa acattgcata gtctttttgc
    27721 cgcatcgtca tcaaaaagga atttgcatat aaaatataaa cgaatgtgat gatccctagt
    27781 aaaacagaac cgaatcggaa aatgataact gtagccgcaa cgatcgtatt acttttcaaa
    27841 aaagcttcgt tgcttgccat tgattcaaac atgtaaaaaa tagcagatgc cataactaat
    27901 cctgagaata acaccaagta gtctcttaaa cggctcttga tcccagtaag tgataatttc
    27961 caaagcatag tcaatctccc cctactgttc caagtttcca agaatagata gtatttctcg
    28021 atagaaggaa tcacggcttt gatctccacg tttcacttct tgatggatac cgccatcctt
    28081 gatgaataaa atacgttggc aataacttgc agaaaacgga tcatgcgtca caagaagaat
    28141 cgatacatga tcttttgtat tcaattcgtc catcgtatct aaaagatccc gggcactttt
    28201 ggaatccagc gcacctgtag gctcatcccc taatagaatc gttggttgtg tgatcaatgc
    28261 acgtgcggcg gcgacccgtt gtttttgtcc accggaaatc tcagaaggat agctttccaa
    28321 aatatgagag atggataaac gttctgcaat tttttggaca cgttgcttga tttcttttgg
    28381 tctgacgcct tgtaaagaaa gaggaacagc gatattttct tgtgctgtca agctttctaa
    28441 taaattgaaa tcttggaaaa taaagccaat ctcattcgca cgaaaatctg ctagttgatt
    28501 tccttttaat gtggtcacat ctttttgatt gattcggata tgcccatctg ttggtttatc
    28561 taatgtggat aaaatattca aaagtgtcga cttaccagaa ccagaagcgc ccatgatccc
    28621 aataaattcg cctttttcta catcgaagct gatgccgctt aatgcttgcg tctttttctc
    28681 attgtttttt ccgtatactt ttgttacatt ctgtacttcg actattttgt tcatttcttt
    28741 ccctaccctt taattagttt ttccagtgct gttttcggca cgtctttttt ttcgacttct
    28801 tcatacgaag tgacaccttt atttttgttc caggtgattt ttaaatatgc tcctttacga
    28861 agcggtcgag tcaattgtcc cttgaagtcc agttcttttt ccttaccctc tttcgtatag
    28921 ccaggcagga tatatctata gttgactgtt tcttgtccag agtctgattt tgaaactgtt
    28981 tttgtaccat ctgtcgtaat ttgtacatag tattcttctc cacccataac tacgtaatct
    29041 gcgattttca cccctgcaag taccacaacg atgaaaacaa gtagaccgat cagaagtttt
    29101 ttcacttttt ccccttctct cttactttat atactgatta tagaaaaaag cgaacaattc
    29161 tgccttcgaa tcaactatca ttcttctttc aattttgtca ttttctttaa aacaactaaa
    29221 aaaagagttc aagacaataa tccgtcctaa actccttctt cctatcttac aaaaagtatg
    29281 tcacttacgg tctttcatgt cgcctgtatt gataacaggt tgcgttcctt tgtctgtaat
    29341 gattgagaca agtaaattat tgatcaattg gactttccga tcatcggtga aattgatttc
    29401 ttgtccatct tcgatttgct ctaaggccat ttgagtgatt gtgacagctc cttcaacaat
    29461 ggtttgtcga gcagacaaaa tggcttttgc ttgttgacgt tggagcatgg cactagcgat
    29521 ttctgttgca taggctaaat ggttcaagcg tgtttcgatt acttcgacac ctgctactgc
    29581 taaacgctct tgcagctctt tttgcaactc gtcagacact gccgtcgtgt ttcctcgaag
    29641 cgtcaaatca ttctcattga atgtatcata cgggtattgg ctagcgatat gccgaatggc
    29701 tgtctcactt tggatttcta caaaatcttg ataatacgct acatcgaata atgcctttgc
    29761 tgtatcaacg actttaaaga ctactactgc tgagatttcg atggggtttc catccaaatc
    29821 attgactttt aaaacagaac tattgaaatt ccgaactttc aacgaaacag tcattttttg
    29881 tgccaaaggt atcgtaagga aaaagccatt ttctcgaatc gtacctaaat attgtccaaa
    29941 aaacaaaatt acttttgctt gattaggact gactacagtt gccgaactta acaacaataa
    30001 cgtgatcaac caaagaaaaa tacttaaaaa aagtgcccaa actttcgctt gccacatacc
    30061 taaatagaag aaaaacaatc caactaaagc taatacgacc aatccgacaa tccctgcata
    30121 accattgata taaaatgctc ttttttctct catgctactt cctgcgctcc tttatccttc
    30181 aataaatatc taatgttact tttttaattt tttttcgaat acgcgccatt acaccttcta
    30241 gttctaactc tttgtcttca gtgatgtacc aaccccttgc ctgtgtccct ccggctgcct
    30301 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    30361 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    30421 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    30481 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    30541 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    30601 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    30661 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    30721 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    30781 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    30841 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    30901 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    30961 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    31021 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    31081 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    31141 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    31201 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    31261 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    31321 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    31381 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    31441 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    31501 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    31561 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    31621 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    31681 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    31741 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    31801 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    31861 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    31921 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    31981 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    32041 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    32101 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    32161 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    32221 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    32281 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    32341 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    32401 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    32461 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    32521 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    32581 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    32641 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    32701 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    32761 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    32821 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    32881 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    32941 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    33001 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    33061 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    33121 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    33181 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    33241 tatcccatta cctaaatgaa caatcagacc aatcgctgtt cctatcacta aagcaaaaat
    33301 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    33361 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    33421 gccaattagt ccgccaagca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    33481 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    33541 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    33601 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    33661 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    33721 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    33781 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    33841 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    33901 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    33961 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    34021 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    34081 atttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    34141 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    34201 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    34261 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    34321 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    34381 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    34441 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    34501 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    34561 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    34621 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    34681 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    34741 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    34801 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    34861 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    34921 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    34981 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    35041 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    35101 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctgc aaaaaaacga
    35161 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    35221 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    35281 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    35341 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    35401 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    35461 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    35521 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    35581 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    35641 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    35701 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    35761 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    35821 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    35881 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    35941 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    36001 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    36061 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    36121 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    36181 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    36241 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    36301 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    36361 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    36421 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    36481 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    36541 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    36601 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    36661 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    36721 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    36781 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    36841 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    36901 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    36961 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacagatca tatgcataaa
    37021 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    37081 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    37141 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    37201 cataatcctg aactggccca tcaatacgca gatcggatca ttgaattttc tgatggtaag
    37261 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    37321 agaacaaaga tgagtttttg gacagcgtta aagttttcct tcaacaatat ccgaacaaaa
    37381 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    37441 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    37501 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    37561 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    37621 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    37681 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    37741 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    37801 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    37861 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    37921 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    37981 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    38041 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    38101 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    38161 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    38221 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    38281 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    38341 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    38401 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    38461 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    38521 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    38581 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    38641 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    38701 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    38761 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    38821 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    38881 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    38941 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    39001 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    39061 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    39121 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    39181 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    39241 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    39301 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctt
    39361 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    39421 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    39481 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    39541 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    39601 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    39661 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    39721 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    39781 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    39841 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    39901 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    39961 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    40021 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    40081 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    40141 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    40201 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    40261 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    40321 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    40381 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    40441 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    40501 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    40561 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    40621 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    40681 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    40741 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    40801 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    40861 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    40921 atgagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    40981 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    41041 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    41101 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    41161 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    41221 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    41281 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    41341 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    41401 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    41461 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    41521 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    41581 tgttaatcac catacaatga aacttttgga tcaatttgga attgtaaatg agcattcaaa
    41641 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    41701 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    41761 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    41821 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    41881 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    41941 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    42001 agattttaga ttggttggaa aaaatagatt tccatatcaa agaaccagtt tattataaag
    42061 atggtgaaaa aacattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    42121 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    42181 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    42241 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    42301 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    42361 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    42421 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    42481 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    42541 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgtcgac
    42601 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    42661 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    42721 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    42781 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    42841 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    42901 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    42961 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    43021 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    43081 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    43141 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    43201 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    43261 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    43321 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    43381 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    43441 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    43501 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    43561 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    43621 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    43681 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    43741 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    43801 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    43861 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    43921 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    43981 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    44041 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    44101 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    44161 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    44221 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    44281 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    44341 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    44401 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    44461 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    44521 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    44581 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    44641 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    44701 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    44761 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccacg
    44821 ccgcaagctt ggatcaagcg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    44881 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    44941 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    45001 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    45061 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    45121 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    45181 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    45241 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    45301 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    45361 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    45421 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    45481 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    45541 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    45601 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    45661 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    45721 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    45781 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    45841 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    45901 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    45961 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    46021 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    46081 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    46141 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    46201 attaaccggt agttcattgg ttggtgtatg aatccactct gccaattgtt ccttcccttc
    46261 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    46321 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    46381 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    46441 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    46501 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    46561 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    46621 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    46681 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    46741 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    46801 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    46861 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    46921 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    46981 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    47041 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    47101 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    47161 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    47221 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    47281 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    47341 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    47401 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    47461 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    47521 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    47581 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    47641 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    47701 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    47761 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    47821 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    47881 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    47941 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    48001 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    48061 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    48121 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    48181 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    48241 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    48301 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    48361 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    48421 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    48481 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    48541 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    48601 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    48661 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    48721 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    48781 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    48841 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    48901 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    48961 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    49021 gttttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    49081 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    49141 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    49201 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    49261 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    49321 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    49381 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    49441 aaactgcgtt atgctttgag taataagagc gctagtcaga aaacagcttc tcatattttc
    49501 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    49561 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    49621 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    49681 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    49741 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    49801 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    49861 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    49921 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    49981 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    50041 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    50101 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    50161 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    50221 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    50281 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    50341 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    50401 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    50461 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    50521 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    50581 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    50641 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    50701 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    50761 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    50821 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    50881 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    50941 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    51001 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    51061 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    51121 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    51181 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    51241 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagaaa
    51301 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    51361 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    51421 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    51481 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    51541 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    51601 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    51661 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    51721 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    51781 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    51841 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    51901 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    51961 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgagattg ggagacggta
    52021 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    52081 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccatgttc ctttaaaagc
    52141 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt tacctgacag
    52201 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    52261 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    52321 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    52381 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    52441 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    52501 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    52561 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    52621 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    52681 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    52741 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    52801 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    52861 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    52921 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    52981 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    53041 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    53101 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    53161 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    53221 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    53281 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    53341 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    53401 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    53461 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    53521 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    53581 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    53641 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    53701 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    53761 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    53821 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    53881 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    53941 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    54001 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    54061 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    54121 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    54181 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    54241 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    54301 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    54361 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    54421 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    54481 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    54541 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    54601 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    54661 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    54721 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    54781 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    54841 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    54901 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    54961 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    55021 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    55081 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    55141 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    55201 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    55261 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    55321 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    55381 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    55441 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    55501 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    55561 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    55621 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    55681 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    55741 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    55801 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    55861 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    55921 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    55981 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    56041 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    56101 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    56161 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    56221 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    56281 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    56341 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    56401 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    56461 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    56521 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    56581 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    56641 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    56701 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    56761 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    56821 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    56881 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    56941 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    57001 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    57061 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    57121 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    57181 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    57241 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    57301 ggttttcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    57361 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    57421 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    57481 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    57541 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    57601 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    57661 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    57721 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    57781 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    57841 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    57901 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    57961 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    58021 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    58081 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    58141 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    58201 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    58261 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    58321 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    58381 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    58441 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    58501 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    58561 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    58621 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    58681 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    58741 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    58801 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    58861 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    58921 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    58981 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    59041 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    59101 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    59161 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    59221 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    59281 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    59341 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    59401 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    59461 attcagatag tcaagatcct gtcattgatt atttatacca aaaccacatc cctttcactt
    59521 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    59581 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    59641 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    59701 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    59761 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    59821 tcttcgccct tcgcacgatg cagttggcgc aaatgtacgg ctatgtcatt ccagagacac
    59881 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    59941 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    60001 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    60061 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    60121 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    60181 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    60241 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    60301 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    60361 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    60421 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    60481 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    60541 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    60601 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    60661 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    60721 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    60781 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    60841 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    60901 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    60961 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    61021 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    61081 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    61141 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    61201 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    61261 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    61321 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    61381 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    61441 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    61501 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    61561 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    61621 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    61681 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    61741 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    61801 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt tatttttctt
    61861 ctaaactttt ttctacctca atcccccaag aatctttctt gaagtgattg atgatcaatg
    61921 gaataacgaa catgaccact gtgatagctg ctactaataa tgcatcactc aatgtcgctg
    61981 tacctaatgt agcaaatacg acaacgatga tagagaagat cagcatgcag ctgactaccc
    62041 aagctaatcc gtttccgttt ttaccaattc ggtaaggacg ttcgaattct ggatgtttct
    62101 tacgcatttt aagcaatgaa acagcaatca aaatgtacac gatacaataa ataacagttg
    62161 tagcatttgt aagtgtcaag aatacagcac ttacgtcatc cattacacca taaagcaaag
    62221 caaataatga aataacgatt gattgtgtta gaacgacatt tctagatacg ccgtatttgt
    62281 tttctttatg gaatccgaat tttggtggaa ggaatccttc acgagctact tggatgattg
    62341 tttttgatgg tccagtaacc caagctgata attgtaaaag cacaccaata aataccatga
    62401 agctgaagat atttccgatc acttctggta agcctaggat ttgacagtca atcaagatag
    62461 gctgcgtgat atttgctaat tccatttttc catctggtac gacatttgct acaagcatcg
    62521 cattgatgac attcaataaa actaatccga tcaaagcgat aaatacgcct tttgtataat
    62581 tttttgttgc gtctttcaaa cgaggcatgt aaacagaact catttcgatc ccaacaaaaa
    62641 taaatgtgat accagctaag tatttcaatg aatcaatatg ttccaagtca gggaaagctt
    62701 ttgaccatga aaagtcacct agatagccgt ttgagactag tccgactttg aaggctgcca
    62761 aaacacctaa aacgaacatg atgactactg ggatatatac ccctagccat acaccgatgt
    62821 tcccgccgac ttttgccata tcgaatttca agttcaagat cgtgatgatc cagtagataa
    62881 ctaaaataca acctaaaacg aaccagtgat tattgccaag ttctacatta ccaaatgtgt
    62941 ttcctaacaa tggtcctaaa gtagaagcga ccattaccat acctgggaac atttgcaccc
    63001 ataatagcca tgcaacgaca aatccccatt tgctgcccag tgctgttttt acccataact
    63061 gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt gatatcggac
    63121 cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta
    63181 acgtaggaat actccgaacc gttgcacaca gtgccattgt cagaccgata aaggtcccaa
    63241 gacccatttt acttacttca gatttactca ccgttgacac tctccttgat tttttggata
    63301 aaataacggt gtctttgaag tagtttcagc aataaacgaa acaattcagc gaatatttga
    63361 ggggattctt tgagtctatt gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg
    63421 atcgaagagc ttaggtctcc ccttgctctt cagatccaaa catgcgtcag aaacagttag
    63481 tattcaagct gttttctaac atgattacct tgcttatttt gcttcgcttg ccaataattg
    63541 gtctgcgtag attttttcca atttttcttg aagagctgct ttgatttttt cagcatattc
    63601 ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg ctggcacgca atacagttac
    63661 ttttccggca cggttccatt cttcatctga gaatcctagt tggttcacaa attgtaatgg
    63721 gctgttgcca tagtctggga tagcaaagtc agtatgtgaa gttaagaact cattaccgta
    63781 aatgcttcct ttcacgtatg aagaataatc atatacgtca tggttcaatt tattcattgc
    63841 aactaagtca tcgttgcctt tttctttgaa tacatagtct accatattga aatctggata
    63901 agttaatgga tgaacttcga tttctttgtc tcctacttta aatgacaagt cattcaagaa
    63961 gttgtagaaa cgatgtgcac cttcgattga tgcacccatt aatttaccat atcctgtgac
    64021 attcaaaggt aagacatggt gtgctgccca aacgctggct gctgttgccc ctgcttttga
    64081 accttccaag atataagccc caagtaatgc tgggatatct gcgccttttt caaatacata
    64141 tgtcgcaaag taagagataa cgtctctcat gcggatatct ttgatgacga taccaccggc
    64201 agaatatgga acatagccca ttttatgtgg gtcaatcgtt acagattctg cttcttcgat
    64261 tgctttatat gcactgtgta cttcttctaa gatataatct ttgttttctg taaagacatt
    64321 gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa
    64381 gaaaatcgca cggccgtatc cgccataagc agcatctacg tgtaagtaga agtaaatacc
    64441 gtctttttct aatacgcgac gcaatgcaac gattttatcg ataccgtcga ttgctccttc
    64501 ttctgttgat cctacaacac cgactacacc taggattggt gttttttcag ctgctagacc
    64561 acgaacgatt ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg
    64621 aatcacttgg tctaaaccga taccgatgat gtcggcagct tttagccatg aatagtgttt
    64681 agtttgtgga accaaccatt ttcctaattt ttctaaatgt tttccgctac gtgctgagtg
    64741 agctttgatt tcgtcgattt tttctgggac gctgtctaac agattcatga tttcttctgt
    64801 tgacaagttc attaattccc aatcgctttt tccagcaact agttcaggtg tcacttcttt
    64861 cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa agtccttcta agtttgctaa
    64921 agaaccatca gcaacgatgt ggccccagcc atctttatag ctcattaatt ttgcaaattc
    64981 cattcctact tcttcttcca tttggcttgt tgcaggagaa gattcatagg caacgttatt
    65041 accattccat agcatagcaa aattgtacgc tagtaaagat ggcatcaatg tttcagagtt
    65101 catatgaccc cagtaacgtc cggcattatg ccaaggaacg gaatgggtac gcatccgcgc
    65161 tgaaatttct gatagaacat cctttgtttt attcacagtg tgttcaaaac tagcactgct
    65221 tttttcttct ggcgtaataa taggcatgtc ttgaggcatg tagttttgac gccagcctaa
    65281 atgttcatcc accaattcat tcaacaaagc tttatagatt tggccgttct cagctttgtc
    65341 cccaatgaat agggcattta ggtttagatc tttcgacaat gattcactca tagtgaaacc
    65401 ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta cacctgttct tctattctgt
    65461 aaactaagtt ttggcctgat ttttaatcat attttaatat gtatcaatta tccgaattgt
    65521 tcacactttt gaaataatta aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa
    65581 agattgtatt aacgacaacc agcgttctat tttaggatgg gtttcaaaat tggaatgttg
    65641 taatagtatt tccctttttc aaggtaaaaa cttactaaat tctaatcaat tactttggcc
    65701 aaaaagtaat ttttcttacc ttttcgaata accacaaact taccatcgaa ttcatcggat
    65761 ggatttatga caaaatttaa atcggtaacg cggtcaccgt taatacttat agctccgttt
    65821 gaaacatctt cacgtgcttg acgtttagat ggttcaattt ttgtggaaac aagcagttcc
    65881 acgatatttt caggtgtgct tgaaatttca acgttcggca ttttaccgaa tccttgcgcg
    65941 atttcttccg catttagttc tttaatattt ccagagaaca atgcttgcgt tattttttgc
    66001 gcttctttca agtcttcttc actatgcaca aatcttgtaa cttcttctgc caaacggcgt
    66061 tgtgcttcac gtttctcagg ttctgtctct acttttttcg ctaagtcttc gatttcttct
    66121 tgagacaaga aagtaaagaa tttcaagtat ttcactacat cgcgatcatc ttgattcaac
    66181 cagaattggt agaactcaaa tggtgaagtt ttcttcggat caagccaaac ggcaccgcct
    66241 gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt taaaccgaac
    66301 gcttctactt ctggtccttc taattttcgg attaggtcta atccagctgt gatatttccc
    66361 cattggtctg ctccaccgat ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag
    66421 tcgatagatt gcagaatttg gtaagtaaac tctgtaaatg agattcctac ttccaaacgg
    66481 ctggcaacga tatcttttgc taacatcgtg ttgatgttga agtttttacc gtagtctctc
    66541 aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt
    66601 gcatctttac caaacaattt tctcatttga ttggaaagtg cgtccacgtt atgttgcact
    66661 tgttccatcg tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct
    66721 gttccgccgc caatcagaat atatggatga tggcctgcca attggaatct tttcatcatc
    66781 ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac gccgcaataa
    66841 agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta
    66901 atggcgtcgc gccaagttaa ttcatcaata atattcattt tactgacctc cgtgtagttt
    66961 ttttatgaaa gcaggaacct gctagcatgt gtaattaatt atttcttcag gataggatag
    67021 gaaataaaaa aagtcccttg gcatataagc caagggacga aatctcgcgg taccacccaa
    67081 tttgtatgtc tagatatcca acatacctct cgaacattta acgctgtcgc gtggcaatca
    67141 ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg tactggtttg cagcgaccac
    67201 cagctttctg aaacattggc tttaacaact acttcgtttc gatcttcgtc ttgttattat
    67261 taatatgtgt ctatcataga gcgcaacttt ttctttgtca acagaaaaag gatatttttt
    67321 ttaaattcct cattttttct tttttgaatc cgctctaatt ccgaacattt tattttcaat
    67381 tattgttaaa agaaataact ttttctgtat tttttctgca atttatcgag aagtcacttt
    67441 actttgcctc ttattctttc ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa
    67501 agcaagggac gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc acttctgctt
    67561 taactatcgt tattttaatt ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag
    67621 aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca gatgataagc
    67681 aacaactagt tactcaagca aaaaatctgg ctcaaagcta cgattatgac caagcgattg
    67741 cgttattgga aaaagatagt aagaacggat ctactgaaac acaacagctt cttcaaacac
    67801 taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc tactcagatc tctcatgtct
    67861 ttttccacag cttgatcgtg gacccgcaaa aagccttcca ctctcaacag gcacaaggat
    67921 acaaagatta tatggtgact gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa
    67981 atgattttgt tttagttaat ttaaatgatt tgatccaaaa agacgaacaa ggaaatcttt
    68041 cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca caagatgatg
    68101 tcagctatta tgaatatatg gataattccg gttttcctag ccgattggtc gtatcaaata
    68161 acaatgaaat caaaaatgtc tataaagaga aagataaaga agaaatcggt gactttgaca
    68221 tggttcctct gatcgatgcc tttgtcaaga aacatcctga tttttcttat cgaggcgcaa
    68281 aaggtacctt ggccctgaca ggttacaacg gagttttagg ttatcgaaca tcaaaatctc
    68341 aatatggaga caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa gtggcggaac
    68401 agttgaagaa agacggctgg agctttgctt cccatacatg gggtcacttg aatatgactc
    68461 aagcttcact agaagatgta aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta
    68521 tcctaggaaa aacaaatatt ctgatttatc catttggtgc agacatcagc gactggcagc
    68581 cttattcagc agaaaatcaa aaatttgctt acttgaagag tcaaggtttc gatattttct
    68641 gtaatgtcga tgcttctaca ccagcttggg gacaattagg tacggatttc tatcgaaatg
    68701 ctcggatcaa tatcgatggc attcgttttc aatctgattt agacggaaaa aatcctattc
    68761 ttgatccatt catcaatgtc catgatgtct atgacaaaga agcacgcgaa tctgcttgat
    68821 tttacgtcat ttatgaacca aaaaagccga actgacattt tatcgtcagt ttcggctttt
    68881 ttatgcttta tttcttcttc ggtgcgattc ccttgatcag gacttctgtc cattctgctg
    68941 tatatggaag cagttttgcg tatatctccg gcttagaaat tccttctacc attgcttgaa
    69001 aatagaggat caagaactca tccgaatatt tcaaatccac ttttccttct ttccgccctc
    69061 gatggaaaag atcaagcatg atactgaaac tttcttgcgt atacgcatcc atcgcgtcag
    69121 aaaattcttt tttttctttt tttgtatagt atgtcatcaa atcgatataa aattgtttgt
    69181 tcactcgatt gagataacgg attttgtttt cactcatggc aatcagtgtt tcttcaaatg
    69241 gaagattttc cgcaatcaac gctttcgctt cttgccctaa atcattcata aatgcaaaaa
    69301 atacttcata aatcagccct tccttgctgc caaaatattt aaaaatcgtc gctttactta
    69361 cctgagcgtt ctcagctacc ttatccattg tcactccagc ggttcctatg tctgtgttca
    69421 tcaagtcaaa agctgcttgt aaaatctctt tctttttctc ttgtgctctc ttttcaaaac
    69481 cgttcatttg tagagcctcc cgtaatttta aacttttata atatatatag ttcaaattta
    69541 ttctgaggtt tattataact atctattgag aaagtatcaa gctagcaatt ccttgtttat
    69601 gaattcattg acagaaattt ataaaaagaa tattatgtac taaaataata aaaaaagttt
    69661 tataaaaagg tataaaacct ttttattaaa ctatttaatc ttgtttagta tatattttta
    69721 gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg aaaaagagct
    69781 ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt gaagtcgttg
    69841 gatttatcgg accaaatggt tctggaaaat ccacgacgat tcgaacactt ttagggatta
    69901 taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa gatagtttat
    69961 ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga aatttgactg
    70021 gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag
    70081 atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag
    70141 gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat ctctatttat
    70201 ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa gaagaagttg
    70261 aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc agtgaagtcg
    70321 agcgactagc ggataaagtc gtgatcatac gcgaaggaac agtcgtagaa gaaggaacac
    70381 tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa gcagagaata
    70441 gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat
    70501 ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga
    70561 aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat tatgaaggat
    70621 gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt tctcaaaacc
    70681 ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct ttttctggtg
    70741 gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga ttatacgaaa
    70801 ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg aatgctgctg
    70861 agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg
    70921 ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga
    70981 ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg
    71041 aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg atttttttcc
    71101 aagcagattc gatcactgca gaaggtgctt ggctttttgg gctatctgta ggtagtatgg
    71161 ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt tcagccagtt
    71221 cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca ataacagatg
    71281 tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg acctatccat
    71341 ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta atcttgataa
    71401 tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc cccgaacgtg
    71461 aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta ttctatctaa
    71521 ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg gcatacggct
    71581 ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa cagatgttta
    71641 tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggtc gtcttgattt
    71701 gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa gaaacccacg
    71761 gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat tggaccaata
    71821 tgttactagc tattctattt gctatattgg caatcctgat tagttctggt gcactagggc
    71881 tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg tttcttaaag
    71941 ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc tttctattcg
    72001 gatggcttcc tcgaatgggt aaacttgctt atatctactt agcttactcc tttgcactca
    72061 attactttgg cggaattttg gatttaccag aatggttttc caaaacagct gtccctagct
    72121 gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg ttatcatgta
    72181 tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac ttactagaag
    72241 gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca aaaatccaac
    72301 atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa tgatccagac
    72361 ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt ttctagataa
    72421 agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg gcaggaaaaa
    72481 gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc gttcttctac
    72541 tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct caacgttgat
    72601 tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga aatctttttg
    72661 tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact catttggaac
    72721 gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta aaagctgttc
    72781 ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga tcactaattg
    72841 aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat gtttgacaag
    72901 tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa caaatgaaac
    72961 acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc taataaagaa
    73021 acataacttc ccttattcca actgataatt ttcttttatc atagcataaa cgtcaagcga
    73081 accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt tccttctttt
    73141 gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg aataatcaaa
    73201 aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt
    73261 tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt
    73321 ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat
    73381 ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa
    73441 atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata
    73501 atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc
    73561 tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta
    73621 ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg
    73681 gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg
    73741 tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca
    73801 tttttgccag tgttctttat attctttctt gatttgttca atcttatctg ttggatggct
    73861 ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc
    73921 aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat
    73981 ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa
    74041 aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat cacatctaaa
    74101 agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc gattgcacgc
    74161 tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg ttcttttgcc
    74221 tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac tggcagccaa
    74281 acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat acccgctgtt
    74341 ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac ctcttttgtc
    74401 gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc accgactttc
    74461 gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg atgatcggct
    74521 tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg gtctccatcg
    74581 ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc ctctgcaaat
    74641 tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc cttttatctt
    74701 gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc
    74761 tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt cataaaatta
    74821 ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa aacatcattc
    74881 atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag tacacctgct
    74941 tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt tgccagtact
    75001 ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc aacaccatac
    75061 tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac agttgctgct
    75121 cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag ttctttcaac
    75181 tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac aaggacagta
    75241 cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat atcatcatct
    75301 acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc catatcgaag
    75361 ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc actatccgct
    75421 tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc accaagcacc
    75481 gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg tccgcttcct
    75541 ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac acgggaagat
    75601 tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat ttcttcgaca
    75661 atattgcttg gatcattcat gatttttttc aatttattcc catcctttct ttccggcatt
    75721 ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga tggctgctgc
    75781 aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct gctcttcttc
    75841 caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa
    75901 ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg ggtctgatcc
    75961 gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt tttgcatctg
    76021 ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa gcaaaatact
    76081 ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt tcgctttcat
    76141 taatgaacaa ttcaagtata taataaattt ctaattttgg gaagatatgt gtagtgcttc
    76201 ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac aacaacaacg
    76261 aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg tgaggtcatt
    76321 actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt tggcggccgc
    76381 tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc cgaaatggcc
    76441 gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga ctatgaagta
    76501 ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt ctccacgaga
    76561 taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt ggcactcaag
    76621 aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc ttttttattt
    76681 ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg ctaaggaata
    76741 actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg atttggatgg
    76801 ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt tttctcctgc
    76861 cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt tttttgcttc
    76921 aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt gactggccgc
    76981 tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa cctgatttaa
    77041 ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt ggacagcttc
    77101 cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat ttcccacttt
    77161 atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca atttgttttt
    77221 tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt gcaaatgatc
    77281 gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg aaatcgtttc
    77341 tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac cgttttcagc
    77401 accagaagag cttatagttg ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa
    77461 agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta acggcttcat
    77521 tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt attataagca
    77581 aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt
    77641 tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa aatcgttttg
    77701 actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa caaaagcata
    77761 tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac aatcagcgaa
    77821 gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta
    77881 aaagtaatat aaatctgttc gtagtttcct tgatatacat aatgcccgat taatccagca
    77941 aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt ttgatcaatg
    78001 aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt gaaattcgtc
    78061 agccagactt ctgttccacc ttttgcccaa cgttttcttt gtcgatatag catcttcaac
    78121 gtcacaggta cttccatgaa aaagataatc tcaggagcaa aaacagataa ccagtcattt
    78181 aactgatgat cccaagcaat actgatgtct tctgtcgctc gatcttgtct gaatccgccc
    78241 acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc ataaagacca
    78301 ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt
    78361 tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt gaccgcagca
    78421 atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc atctggctcc
    78481 ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc aagtccgata
    78541 ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag attagcgtat
    78601 tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc gtctgtcact
    78661 aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt atcttcgatc
    78721 acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac actcaaggga
    78781 acatctaccc attctttttg cttatgtttg tataaaaaca cataacacca gacaccaata
    78841 aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaatacata atagacaaac
    78901 aagtatctaa gaataaatta cctgtatggg tgattgagat catttcagtt ccaccccatt
    78961 ttctttatac aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg agacagaata
    79021 aaaacggacc gtttctctct cttcttttgt tccaaaacgt tccgtataca cttcttcaag
    79081 taattcttgt ctttttttca atttttcatc ttcatattca aaagatttat ttactttttt
    79141 gtatagattt cgattgttcc acctagtcaa tacaaatgag gtaatcagga aactgaaaaa
    79201 aatcaataca aaaaagccga ttaagaaata cagcattttc ttttctgata aaaaagcgta
    79261 caaggaaaaa tccttggctc tttctggtct cgttaatggg aaaaagaccc atataaaagg
    79321 aagaacaacg atcatccatc ctaaaagagc cactaacgtt tgccaaatct ttaaccagta
    79381 attacctttt gaaaaatact tatctttcac caaaattgat gcaacttctt ctcttgttac
    79441 ttttttcata tcctcacctc acgttttttt gttacgacgt aggagtttac catacatttc
    79501 atttcttttc acataaagtg ttcctaaatt tttcctaaga aaaagaacaa aacatttctc
    79561 atcagcaaat gaaaaatatt ttgttctttt tatcagttgt tctttttatt ttttatctct
    79621 taattatgtt gctctttcca atacgacaat gcttttcatt cctctagata tcgccgtgta
    79681 catttgtctg cgtcttcttt gctcatctcc atcgttttca aatggatata agattacatg
    79741 atcaaattcc agtccttttg ctagactcaa aggaatgatt tggcagtttt ctaatccata
    79801 tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt gggtgataac
    79861 tgccgctttt tctcctttta gagaaaccaa aatctcagct agtattttca acaattcttc
    79921 agaggatcga aaagagcggt attcgggttc ttttccttgc ggtcggatcg atacgatgtc
    79981 gatcttctct tgatccacag cataggtttt gaaaagctct gtgattgctc cgctagaacg
    80041 gtaactattt ctaagatcat accgatggat cggcagatgg tagtcgtcga aacaacgcat
    80101 aatatcagca aaagtagtgg aagaattaaa gattgcctga ttctcgtcac ctactaaagt
    80161 aaagtgagtt tttggaaata attcgatcaa tagacctaat tgtgccaggg tataatcttg
    80221 tacttcatca atcaatagat agcgaaatgc tggtacattt accttttcaa caagtaaatg
    80281 acgaatagct aatataatga ctgcttcatc aacagtacgt gtgggttgcc aagcataagc
    80341 tgagccatat cgtttttcat aaattctttc aaacaactga tgctcatcca cccatgccat
    80401 ttcttcgatt tgtctggtta tttttcgata ttttttttgt aaaagtttcc ttgcataagc
    80461 tacaatactt tgttccgaat catcagaaat caatttaccg aataattcca actgcctatc
    80521 ttctgtcaac gagagtacct gattctgcac agcaggactt tttgcttgtt tcaaaagatg
    80581 attcttccat tcgctcatca gtgccttttt cgttgcttga agcctatcga tcaatttagg
    80641 atgagcagga actttctcat atattttttc gatcgttttt ttagaaagga ctgtttttcc
    80701 atttttagta agtccacgga taaaattcgg atgatctaaa aacaaaggat ctgattgttt
    80761 tagattatcg ataaactttt tgctacgcaa acgttctgtc tgctccgata cattttctcc
    80821 cgtgatacgt ttgaaataag cctcttcacc ttctatttcc tctgctgaaa gctgtgaaac
    80881 cagctgtatg atggttagat tcagtggatt tctttcacct aatgatggta ggacattcga
    80941 aatatagtcg ataaaccgat gattaggtga tagaatcagc aagtcatcgg atgtcatcgt
    81001 atctcgatat aaatagagca gatacgcaat acgctgcatg atagcagagg ttttaccact
    81061 tcctgctacg ccatttacca agatattttg acttttcaca tcccgaatga ttgtgttttg
    81121 ttccgattgg atcgtagcag tgatagcctt catttcattg gtctcatctt ggcctaagac
    81181 ttctaataat acgtcatctt gaatagctac agacgtatca aaaaacttga ccaaacgatc
    81241 tctttccaaa agaaattgtc gtctttcttt aataaccgta tcgattttat tttgacgaac
    81301 cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg gtgaacgcca
    81361 atcataaaca agattttcat ctgcatcatc tgtgaaattt gctgtaccga tataaaagga
    81421 ttcttttggc tcattatcca gaaagtctac aacgactttt ccaaagtagg gacttttcaa
    81481 taaacgttcg atctttgcta gctgattttc tgcgtttttc tgtttgatat tcatctgatc
    81541 gatttctcta tttttcattt caatcatcga aaaagtatcc aggttatcta agtaactgtc
    81601 aaaattcaat cgaagctcgc cagccatttc tttcaacgac tggtttcctt ctatttttgc
    81661 cgtatccatc gattcccgca gttcgttttt tttatcaatt agcttttgat acacgttctt
    81721 cagatatgct tcttctaact gtttttgtgt gttgaccata cacattctcc tcttttttta
    81781 aagtggtgga accgattagt ttacctcttt taagagtcga atgcaagctt tttaacgagt
    81841 atcatttgtt aggatatatc agttcttcag gtcggacagt ttgatataaa gaaaatgcac
    81901 catcaaggtt ttttacgtgg tagccagact gcttcaagat tctttccgct agatagctac
    81961 gtaatccgct atgacagctg acgatatatt cttgttggct gtctaattca tttaatcggt
    82021 cccgaagttc attcaaaggg atatgtttgg cttccttgaa tttgcccttt tccaattctt
    82081 ctgctgttcg cacatctaat aaaatttttc cttttgctag ttctgtcggc aattcatgcc
    82141 attgtacggt ttcactaagc ccttctgcaa tgttcattgc tgcatatcct agcatgttta
    82201 ctggatcttt ggctgagcca aatggcggtg catacgtcaa ttctaattcc ggtaaatcga
    82261 agatcgtcaa atgccctttg attgctgttg ctaaaatatc gatccgttta tccacacctt
    82321 ttgccccaac accttgtgca ccataaatct cgccagtctc tggatggaag atcaacttca
    82381 gtaaaatatc tgttgcttct ggataatagc ttgcatgatc ttttcctgat acatgaatga
    82441 cttcaaaaga cagttcgttt tctttggctg ttctttcact taaaccggtg gatgcggcag
    82501 tcattccaaa cgttcgtata attgctgttc caataccgcc ttgatttctt cgagaaattc
    82561 cggaaatcgt gtccgcaact tgtctacctt gacgattggc tggtgaagca agagaaataa
    82621 gtgcatcttt tccagtgatt tgctgtttta caacgatagc atcccccacc gcataaatat
    82681 caggaatatt cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg cctaattcga
    82741 ttccagcggt cactgctagc tggttatcag gacgaacacc aatggataaa ataactaaat
    82801 cagcagggat ggtacgccca tcatccagtc gcaatcgttt cccgtggtct tcaaatccag
    82861 cgacagcatg agaagtaatc acttctactc cttgatgaga caattctttt tctataaaag
    82921 ctgccatttc ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc accatgactt
    82981 ctaaacctct tctttttagg ttttctgcca tttccaatcc gatgaatcct gcaccaacga
    83041 tcacggcacg ctttggctgt ttttctaatg catgcatcac tttatcgata tctggtacat
    83101 ttctaataga aaaaacattg tcggcttctg ttatccctgg aatcgatgga acaactggtt
    83161 ttgcaccagg agataaaacc aatgcatcgt actgttcaat gtgtttttga ccctcatgta
    83221 cgacttctac cgttttgttt tcgggaaaga tggctgtcac ttcatgattt ggacgcacat
    83281 ctaaacaaaa acgagcggat aatgattctg gcgtttggac aagaagattt tcccgttcac
    83341 tgatttctcc cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca tacggtcctt
    83401 tttcaaaaac aacgatttct gcatcttcca ttaatcggcg aagccgtgta gcagcagaca
    83461 ttccacctgc tacacctccg acaataacga ttttcatctt atttccctcc tactgttcga
    83521 tcaaaccatt gattcatgcc accacgtaca ttcactacat cataaccaga tttctttaat
    83581 tctttagctg cttgtttact cctcatccct gattgacaaa tcacatagac ggtcttttct
    83641 ttgtctccat catatctgtt tatactttgt aatggaacat ttttagctcc ttttatatgc
    83701 ccgccccggt attctgcagg tgttctaaca tcaagtaacg ctggattttc caaaagcttt
    83761 tcctttaatt ctgggactgt tatcgatttt attgattgaa atagcaaatt cattttctgc
    83821 acctcattta catttttata aatacctcat ggggtatatt aaacaggagc tttattaata
    83881 tgtcaaccag aatgactctt catttttttc actacttttt atatttgcat aaaataccga
    83941 taaggggtat tatctaatta taaaaataga ggagaatgtg ttatggagat tgataataaa
    84001 aatatcctga accgtttgaa acgtacagag ggacaaattc gtggtatcca aaaaatgatc
    84061 gaagacgaaa aagaatgtat ggatgtcatc actcaattaa gcgctgttcg ttctagtatt
    84121 gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa attgctttga gaaacctgat
    84181 agtaatccag aagaacaagc ggctaaacta gaacaagcga tcaagatgat tattaaaaag
    84241 taaaggaatg atttcaatga aagtcgtttt ccatattgat gaattagaaa aatggtcaga
    84301 aacagggaaa aacgtaaaaa acctgataaa agcttctcca gaaacgacga ttgtcgtatc
    84361 tgtaaatggc atcgcgatta ccggttatct agactctgcc aatgcagaat tcttagattt
    84421 gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt gctaatcata tttcagaaag
    84481 ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag ttgaactgca
    84541 atcccaaggt tatgcttata tcaagcctta gtcagtagag gttgcgacag aagtgatcag
    84601 cttcaagaaa tttcggctta tttttcgaag aagctacttc tggaaaaccg tgaatcaaag
    84661 gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcttctca
    84721 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
    84781 agaggttgtg aaatcagcgg tttgacctaa gtattagaga aaaaaacggg aaaatagctc
    84841 ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttaag
    84901 gaagagattg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatgga
    84961 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt
    85021 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat
    85081 agctgctcat attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt
    85141 ttaccaagag gttgtgagcc taccatttaa ctccgaatca caagaaataa tttcaaaaac
    85201 aacttctcct gtttttggtt tggttggctt attatcgaga agtagctact gggagtctat
    85261 ttatcaaaaa aaggaatgcg acataggtca cacccctttt ttattttctt tgaaacatct
    85321 ttcggtactc gacagcatac aagacaaaag attgtaaaag accgtaagtg atgacggcaa
    85381 aaatgactct cacccacagc gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa
    85441 catagatgac agcagtcgct actttgccat accacttcgc tccacttaat ttgatatttt
    85501 tagatagccc gattaatcca gctacaaaca taaagccttc ttttatcaag aatagaccaa
    85561 acaacagcca aagccacggt cgataccatg ccatagacaa aatcagaaaa agttgtgtca
    85621 atttatctgc aaacggatca agcaccttcc ctaagtctgt tacctgatta tatctccgag
    85681 caatgacccc gtctagataa tcagacaaac ctgagacaac aatgaccgct cctgctatat
    85741 aaaatgactg tctcagcaca aaataaagat aaatcggcag caaaaaaata cggaatatcg
    85801 ataaaagatt cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat ttcattttat
    85861 cgccctacct cctcagcact cttttctttt tattttagct tacacgggga ataaaaccaa
    85921 tgatttcact gtttttattt ttctttacag ccactgatat tatgagctaa tttgagataa
    85981 tcacttgctg ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca tttcttttac
    86041 tagtacttta tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt
    86101 ataatcgata aacagactgt ctaaatcgta acctgtcggg tacagatctt ctgctgaacg
    86161 caccagacga acacgctgac gaggcacgtg gattattttt ttgtctaaat atacatcaat
    86221 cgtatcttca cctatatctt cataaactaa cgcttctttt tggtattcat tgacaaatat
    86281 ccgatcacct tttgcaaaga gctggcgttc ttttttattc tccttcctat ttgtaactgc
    86341 tgtcttagga agaaattctt ttttctgtgt ccgataggct ttttccgcca agtaacgctc
    86401 tgcttgatgg atcaatttca tcgacatatc cattttatga gcaatccaca aagcttggct
    86461 ttctcctgtt tctccaaccc gcaactgata tttaggacga agtgcttccc gatcgaaagc
    86521 catagcagcc ggcacaaaat cttcatgatc tccggcgaat ttcttgattt ctccataatg
    86581 ggtcgtggca atgatcaatg ccccttgctc atacatagat tccatgattg cgatcgctag
    86641 tgcagcccct tcatttggct ctgttccact gccaatctca tctaaaagta ccaacgtatt
    86701 cctttttatc tttttcaaaa tagcagcgat gttttgcata tgaccagaaa atgtactcaa
    86761 cgcattttct aaattttggt ggtcaccgac atcgacgaaa atctcatcaa agacagcaag
    86821 ctctgtccct tcttttgccg gaacttgcat accgaacatc gccatcaaag tcaataaacc
    86881 gactgtcttc aacacgactg ttttgcctcc ggcatttgct ccggtgataa tcaagcctcg
    86941 atagtcttta ccgatttcaa catccaatgg taccgcatgc tcaggtaaaa atggatgtct
    87001 gccttgcttg ataatgatat gttccgattt attgattcta ggtgtgcttc cattgatttc
    87061 tcggctaaat tttgcccgag caaagatgat atctaacacc gtgatcgttt cgatcaataa
    87121 atcgatcact tcttcgtttt ctgcgatagc accagtcaat gccgccagga cttgatattc
    87181 ttcactgatt tcttcagctt ttaacaactg atagtgttca ttcaatttag aaatcacagt
    87241 tggttcaata aaaaccgtcg ttcttttatt tgattcatcg ataatcgttc catctatttt
    87301 atttttatag ctggctttga taggaatcgt atagtattcg cctttttgaa caatcattgc
    87361 ttcttggatc atttctttgt ttttcggatg acgcaagaat ttcagtaatt ttgactggat
    87421 ttctttttcg attagttgta attgtttgcg tactttacgc aaattacgtg acgcgtcgtc
    87481 actgacttta ttatttttta ttttctggtc aatcaactct tctacattta tcaaatcagg
    87541 aagatgtttg ctataagcaa acaacaaggg tgtctggtat tgattcttat caaaaaactt
    87601 tgtgatcatc cgactgcttc taagaaaatc cgcatattcg atcaaatcgg ttggctgcag
    87661 aatcaaccct tttttgactt gttctgtcag tgcatcgatc ctcggcaacc ccataaatgg
    87721 aacatgctga ctgctttcta agatcaagcg tgcttctctt gtttcttctt gacgatccaa
    87781 gacagtttgt aggtttgtcg agaccgtcat atcgctgatt cgttttttgc tatagtttcc
    87841 aattgctctt gcttggattt cctttttgat ccgatcaaat tgtaattgac taatcatttc
    87901 gttgtccata ttctctcctt ttcttgaaga gatacctgtc ccaaaataaa taagggccta
    87961 gagataaata aaaggataga acacagctta aaaaagtcat gtcctatcct tattagaata
    88021 caaagatatt gttttcctga caggtaccgt ttcataagca caccaaataa actttgttta
    88081 ttggctattc gacttactga ttagatacca gctacactca caaaacatac cccattcttc
    88141 taggtcttat ttattttcca cttaactata gcaagacaca ttttggttgt caaactttag
    88201 tatgttttat agatttacac cattaatgaa tagacaccta ccgcgataat tgctccgatc
    88261 attggacctg cgattggtac ccatgaatat gcccaatctg aagttccttt attagctatt
    88321 ggcaataccg catgtgctaa tcgaggacca aagtcccgag cagggttgat tgcatatcct
    88381 gttggccctc ctaatgaaag accgatggct aagatcaatc cacctacagc gaatacgttc
    88441 gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc caagaactaa tacaaatgta
    88501 ccaattaatt ctgtgatcac atttgctgga taattccgta tagcaggacc tgttgcaaac
    88561 gttcctaaaa tcgcgctttc atctttcgtt gcattccaat gtggcaaata agaaagccaa
    88621 acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa tgatgaaagg aacgacaaga
    88681 ttccaactaa ttgcccctgt catcgccata gctagagaga ctgcaggatt caaatgagct
    88741 gggcccatga atccagaaat atagacagcc attgttacag cgagtcccca accaaatgcg
    88801 atgacaatcc agccagaagc ctgtgcttta cttttgttta aattcacagc cgcacacaca
    88861 ccatctccaa gcaaaactag gatcagtgtc ccaataaact cccccatgat ttgagtcata
    88921 tcactgttca tttatttctc cccctttagt tcccgcagat ctgattctgc aatcaatgct
    88981 tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc attcaaaata tccagcaatt
    89041 gcatcgatca caggttgctt gatctcatca agctgatctc gttcaaacaa cagatgattt
    89101 gtacgacgaa tgagataatc gcctggggcc aaaaccattt cttcttctaa accataacgt
    89161 aaccgagctg actcagcgag actcaaacca ggataaggtg ccatttcttt tgctaattca
    89221 aagatacgtc gggcattagt cccgtaaaaa tcagcgatat aagtagcatc ttcttcctct
    89281 aaaccggctg ctactccttc ttttgctaat tctgtcactg tttcttctaa tttagttgga
    89341 tcgaagtttc cacctgaaat ctgatatttt ttagagtcga tttctttcgt ctctattccg
    89401 tattcttctt ttaataattg gcgtatcaat cgcaaagctc cttctgccat cttacggtaa
    89461 tcggtgattt tcccaccaga taaagtcagc aaaccatctg gttctctttc taaagaacta
    89521 cctctggaaa tcgtagaagg tgctttttca tcacggctgt tttccaaatg gttcaacacg
    89581 tcttctactt cagcacgaga aactttgttt tccttatatt cacttactgt atcgacaact
    89641 ttattgaagc tcttgtctga aatcgatcca ttatctccac cattgtaatc agaaccagaa
    89701 ttaccaatca acagtggacg aagtcctgcc cagcttgctt cgatatcatt caatgtgata
    89761 tttgcttctg gatagcgatg attgatcaca tccaatagat aatctacatc ttcttgtgtg
    89821 actttcggat ccgtaaaatc tccttggtaa tccgtatctg tcgtaccaaa gtaagtcttg
    89881 ttttctcttg gaatagcaaa aaccatccgc ccatcttgct ttcctgtatc gaagtatgtg
    89941 ggttgcggta caggcagttt tttcgcatct acaactaaat ggatcccttt agttggacgc
    90001 attttaggag agactggacg cgtaaaattc aagtttcgta ctttatctac ccaaggacca
    90061 ctcgtattga tcactaattt tgctttgatt tcgatcactt catctgtcag caggtcacga
    90121 gctttgacgc caacgatttg atcgccatca tataaaaagc caaccgcttt cattttactt
    90181 actagataag ccccatcttc ggcagccttt ttgatattat caatcactaa acgggcatca
    90241 ttgttgcgga aatctagata gacacccgcg ccttttagcc cttctttttt caaaaacggt
    90301 tctctttcca atacttcttc tggtgtcagc gtataattct catatttggt tcctgtcaca
    90361 tttgccagtt tgtcataaag gtccattgct actttgaccg agaacatatt gaaggttgtt
    90421 gctccttcgc cctcatagat tggcaaaagc attggatctg gttttgggat gtgtggggca
    90481 atactttgta cgactgcacg ttcaccaact gtgtcagcta ctacttccac atcaaatgtc
    90541 ttcagataac gaataccgcc atgaacaagt ttggtcgagc gagaggaagt cccttctgca
    90601 aaatcctgca tttcgatcaa tcctgttttg attcctgatg ctgctgactg gactgccaca
    90661 ccggcaccag tgattccgcc accgatgatc aacagatcca attcttcttg ctgcattttt
    90721 tgaatgctat cttgtcttgt cttgtttgaa aacatatgga tttcctcctt attcaaatgc
    90781 ttgggtagct tttactgctt ttttccaacc gttgtaaagt ttttctcggc gctcttcctc
    90841 catttgaact tcaaatagct tgccagcttc ataaaattcg cggatctcgt ctgtatcttt
    90901 ccaataaccg acagctaatc ctgctaaaaa tgctgcccct agcgccgttg tttccaagtt
    90961 ttctgctcgt tggatcggta cattcaagat atctgtttgg aattgcatca gatattcatt
    91021 gtttgctgca ccgccatcta ctttcaatac aggaatcttg attccagtgt catcttgcat
    91081 cgtatcgatg atatctctca cttgataagc aatcgactgt aaagtagctt taatgatatc
    91141 ttcttttgtc gtaccacgtg tcaagccaaa catagcccct cgtgctgcct gatcccaata
    91201 aggagcacct agcccaacga atgcaggaac aacataaacc tcatcttcgc ttgtagagcg
    91261 tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt tgaagtccat ctctcagcca
    91321 ttgaatcgaa gaaccagcca caaaaatact tccttctaaa gcatagtaaa cctttccatt
    91381 tatgccgtaa ccaattgtcg tcaataaatt atttttggat aattgcggtt tttcaccggt
    91441 attcatgaca ataaatgaac ctgtaccata agtattcttg accatgcctg gttcgaatgc
    91501 catttgtccg aataatgcgg cttgctggtc tcctgccatc cctgaaattg gtgcttcgct
    91561 tccataaaaa tgataacctt gcgtataacc ataaacttct gagttagaag tgacttttgg
    91621 taacatcact cgaggaatat ttagtaaatc taaaatctcc tgatcccaat ctagctgatg
    91681 gatattgaac aacatcgtcc gactggcatt cgagtaatca gtaacatgtg cttgtccatc
    91741 ggtcagtttc catactaacc aagaatcgat cgttccaaac atcaattcgc ctttttcagc
    91801 tcgttcttgc gcgccttcta catgatcaag aatccagcgt atcttcgttg ctgagaaata
    91861 ggcatcgatc actaaccctg ttttttcgtg gaaaaattct tgatagcctt cattttttaa
    91921 gttatctgca attccagctg actgtctgga ttgccaaaca actgcatgat agattggacg
    91981 tcctgtatct ttttcccata ttactgttgt ttcacgctgg ttcgttatgc caatcccagc
    92041 aatctgattt ggtttgatcc ctgattcgat gaacgctccg gcaataactg actgtacaga
    92101 attccagatt tcatttgcat catgttccac ccaactttct tgcgggaaat attgcgtgaa
    92161 ctctttttga gaactgccga tgtgtcttcc tttcttgtca aagataattg ctcgtgagct
    92221 agttgtacct tggtcaatcg ccatgatata cgttgattct gtcattgtga ttccctccat
    92281 tatatgcaaa cgctttcttt aactactctt atcatactac tgttttaaaa aaagaaactg
    92341 tcaaaatttt attctttttt tctttttttg acagtttttt attatgaagc tattttttaa
    92401 tcaattcttt tcttttttca gcgtctctat tcttcttttc aatcgaataa tgtcgtaagg
    92461 tgacgcaaat tctgacagat aatattcaca ctgttttttg tttctctggc aaaatgtgat
    92521 caccaaatca tattctttac cttcttcata acttccaatc tcaataccgc cgattcccct
    92581 cagttctctg atcagatatt gttgaaaagc aatacgataa atcggcagac tttctaaatc
    92641 gattccaatc actactggtt ttgcaatata aaaatcgatc atcaaaagaa cattggcata
    92701 ctggatcatc aggtaactaa gctcactatc ttcagctctt ttcctcggca attgctgcac
    92761 cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa tgcccgagca ttttctgctg
    92821 ctgttccaaa agccgatcac gctcatagac ttcgatcttt ccaacaaaga aaaaccattc
    92881 attgttgatc tgggaaattt ggtagcctac tgccttttct tctttgattt tcaaacgacg
    92941 aggacgataa tgccataaca tcgtttcccg aatgtaagta tctaaaacag cggttggtaa
    93001 ctttttgctc cgtgtcaaat catactgata aaatatttct tcgtctacta tcgaaaaaga
    93061 aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc agttctgctg ctgttcgact
    93121 aagatgcatc acgatgattg gatcgattgc ttgataaagc cggtccgact gatagagtaa
    93181 tttcttttct ttcaaagttg cgtacgtact tttttcattc aatagtcttt ttttcgtaat
    93241 cccgagccag caagcaatct gtgcttctgc tgatgccgta aacgttgttt ctaatacacg
    93301 gtccaatcct agaatgaacg gtcttttttc tggtgtattt tgcaaaaaaa gcggtcgctg
    93361 atcttccggg atatattgga atagttcata ataaaaatat cgtatctgta gctcttctcc
    93421 gtataattga ccgtttttga tttgtaatcc aaattcctct aagagctggt tcaattcttt
    93481 gatctttcga aaaacagaag actcactgat attaaacgct gtcgctaatc gaacgatgga
    93541 tacttgctga tgttctaata aatagacgag catcttaaac ttcaagcttt cctgcaataa
    93601 aaacaataga attttttcca aacttacatc ttcgtccaac acaagcagcg cctgatactc
    93661 atttcgctgt acttctactt ttttttccat cattcggcca atcaattcaa tatcttccaa
    93721 atattgatca aacgaggctt ttgacagttg gacatgctct ctcaaaccat tgatagaggc
    93781 tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc tgtaactgct cttttctttc
    93841 taataatgct tcaatcttca taggcagtat tcgccacctt tgaccacacc atatctggca
    93901 cacggtattc tggctggaaa aatagctcat gttcttctaa ctgttcaaac gtccatcctt
    93961 cttctactgc aattgtcagt tggtcaagaa ggtggaacat ctcttttttg ctttggagct
    94021 gtgctccaag aatctttttt tcagaatcaa atagaaattt gagtttaact ttgctttgat
    94081 ttattgctgt aagcttgtag tctacgtatc ctgtttcaat tttttgggga taaaaaatgc
    94141 tctcttcttc tgtcaaacct acgcttccta agaaatagcc aaaccactga ttgccgactg
    94201 gacgataggt tccctgatcc tttgtctgtg agccactaat tgtcttgctg acatttcgtg
    94261 ctgtacggac agcgttattt actagagaaa catacatttt ttcatttgtt ggacgaaaag
    94321 ttacttggat cgcatcgcca atcgcataaa cgtctttttg agacgtttgc aagtagtcat
    94381 ctacccagat cgttccatca tcattcaatg ttaattgttc ttcccacatc tgattgtcgg
    94441 ggcgagaatg attagccagc aaaacaagat catagttctc ctttcttttt tccgtctcta
    94501 caatagcggt ttcattttcg gtcaaagaca caacttgttc ttctaaaaaa agtgaaagcc
    94561 cttgattttt catttccttt actaacggtt ctgtcatttc aggatctaga taacgaaaaa
    94621 gaattgactt tcggctttcg taaagatgga tttgtttttt tcttgatgcc aacccttcag
    94681 ctatttcgat tcctgcttgt cctgcaccaa taattgcaat tttctttgca ttcatgatct
    94741 tttgctgaag agccgttacg ttttgtatct ttttaactgg gtgaataagc gttgattctg
    94801 cagaaatatt tcgaaacttt tgatttgaac cagtcgctag aatcaaacga tcaaaatcca
    94861 atttttttcc tcctgctaaa acgacttttt tttctttcaa tcctattact gtcttttctg
    94921 tgtaaacatg gattcgatag cggtcagtca gttcttcttt cgtgatccaa gaaagttgtt
    94981 tttcactggt aacctttcct tttaatatca aattgaaccc gctgggaatc catcctaact
    95041 tcgattgctt ttcaatcaaa tatatttcac ttgttggcat caattggcga agataaatag
    95101 ccgcatgaat ccctgcaaac gacccgccga caattacaat gttcatctgc attcctcttt
    95161 cttataaggt gttctactgc taacttctca atcagaaaac acagtattca taactcgctt
    95221 tcaaccttat gatacatagc gatgcagcag aatcaaccct ctccatcata ccgtgttcta
    95281 ggtctgcttc tcaataatgt actagtggac ctaattgaag agtatcattt ctgaagagat
    95341 gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc ttttacgtac ctctgtccaa
    95401 ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg tgtaatgtaa tagttagaat
    95461 agacacatca tttattttta attctttaat gcttcaacaa ctgcttcgcc taattgatga
    95521 gcagattgag gattttgccc tgtaagaagc cgcccatcaa tttgaaccac ttctgaaaaa
    95581 gctgctccag actgatagtt tgctcctttt tctttcaaag catcttctgc taagaatggt
    95641 acttcattcg ttgtaccatt tgcttcctct tcttcattac tgaacccagt cactgtacgt
    95701 ccagctaaaa tagattttcc agactcatct gtaactggca acaatcctac aacaccgtga
    95761 caaacagctg agaccagccc ttcatttcga tagatttgtg aagcgacttt cccaagatct
    95821 attgcttctg gaaaatccca cattgttccg tggccaccag cataataaac cgctgcataa
    95881 tcatttgcat ccacatcatc aggacgtaag gtatttccca aggcccgatt tctgaaatct
    95941 tcatcttcat aataggccca atcgatttca tccatattca aaacactttg cggttctaaa
    96001 ggtacatatc cgcctttagg actgacaaaa tctgcatcga tcccattttt tactaataca
    96061 tcataaaaat gagtcagttc acttaaccac aaacctgtag gacgctcgat cttttcgtac
    96121 ttttctacat ttgttaatac gatcaatact ttcattttcc catcctcttt ctataaaaat
    96181 tgcgttattg ataacgtgct ttcaagaaat tatataatgg gcttggcaaa taaagctctt
    96241 tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc acggttttcc aataatttct
    96301 ctgcccagtc aggatcgacg atcatttgct gtccaattgc tgcaagttct gcatatttca
    96361 atacctgctc tacatcttcg cgattgcgga tcccaccgac accaactaaa gggatctttc
    96421 cttgaatcgt gttatggacc acttcaagaa tcgatttatc gttgtattta tcagaacgag
    96481 ctattcgatc atacatattt aatgaaacat gcagataatc tagttttgac tcacgtaatc
    96541 gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg tgtttcatat tcttctggtg
    96601 agaaacgata ccctacgatg aatggttttt tagcatattg ttcaaccgct gagaaaaccg
    96661 ttgttaataa ttcttcaata aaacgataac gattttccaa gctaccgccc cattgatcct
    96721 ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa atacgtattt gctccatgca
    96781 actcgattcc atcaaatcct gattcaatcg ctctacgtgt ggctttgcca aatgcttgga
    96841 ttgtttcaac gatttcttct tcactcattg ctcgtggtgt ttccgcatca ggacgttttg
    96901 ctgcaactgc acttgcagat acaggctgtt ctccagataa agttgctcga cttgtcattc
    96961 gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc tccttggatt gctttcgcta
    97021 gctcacttaa tcgaggaatc atttcatcat gagcgatgct gagttcacct ggccagcctt
    97081 ttccgatatc ttggacattt gcagcaccag taataacagc accaagacct ttagaacgtt
    97141 gtttataata agtgatctca tcttcggtga tcactccatt gaaaaagctg agttgtgtcg
    97201 tcataggcga cagcatcaat cggttcttca attgaactcc tgaaggtaaa gtaattgatt
    97261 gatcaaattg tgacattgtc attcctccca taattcgctc tgattaacct atcgagaatc
    97321 atctatcatt aaaccactcg attagttgta catgcaacta atttgcttaa aaaattttat
    97381 tttttttgta acttatctgt tgcatcattc attgtctggg ctaattcttt acttgcttca
    97441 tttcccaaaa ctgctttgta tgactgatga agttcttccc agctaccatc gacttcctta
    97501 gcaaattggc gtccttgttc tgtaagataa actaatgaca agcgaccttc gctaactgtt
    97561 ttgacgtatc ctttatattc taatttttca acaaaacgag caatcgttga tggggcggaa
    97621 tagagcattt gtgcaatatc tttttgtggt attccatctt tttcttttaa tatcaataaa
    97681 ataaaaccat aagttggtgc cagacctgtc cttctaaacg cttcatcagc taatttattc
    97741 aacatgcgat ctagcttttt cacgctaaag aaaagacatt catctaacgc tttttcaatt
    97801 ggcttttcca tttcatcccc tccattggcg tacatatcat agtatgattt ttattttttg
    97861 tcaaaaaaca cggaataatg tccgcctcct ctgtctagat ttgaaaaaaa gaagtagcca
    97921 tcttcaaaga atttcggctt gttttcgaca aaactccttc tggaacaccg tgaatcagag
    97981 gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat tttgtgaaaa tggctttcca
    98041 catttttgca taaatttggc ttaataccga aaaactggct tttgaacacc gtttatatca
    98101 gaggttgtga aaaagccgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc
    98161 tcatattttg tcgttttttg agctaatgcc gatggtcagc tgattgtaca ccgtttattg
    98221 agaggtcgtg agcctgccgt tgaactccga gtcacaagga gcaatttcaa aaaacagctt
    98281 ctcatgtttt atgactatgg tgacttgtga tcgaggagtt ggcaggagaa tactgtttat
    98341 tcgtaaagaa ggggctgtga caagactttt gtcacagtcc cttatttcct tttattatag
    98401 gatttcgcca tttgattgga tgacttgtct gtaccaatca aatgattttt tctttaaccg
    98461 tttctttgtg ccattccctt cgtcatcttg atcaacatag atatatccat aacgttttga
    98521 catttgtgaa gtagaagcgc tgacgatatc aatgcagccc cacgacgtat aaccaaataa
    98581 atctactcca tctttgatag cttccttcat ctgctcaata tgctgtttca aatagtcaat
    98641 gcggtaatcg tcttgaacgg ttccatcttc ttctactttg tctatagctc cgagtccgtt
    98701 ttcagctatg aacagtggtt tttgatacct atcatataat tgattcaaag caattctcaa
    98761 acccttagga tcaatctgcc agccccattc acttgatgga agatacggat ttttaattcc
    98821 tccagttatt aagtttcctt ggaccccttc ccaattttct ggatgagcac tgactgcact
    98881 agacatgtag tagctaaaag aaacaaaatc aacagggtag gctgctaaga tttcttcatc
    98941 acctacttct atttttaagt tgacaccttt ttcttcaaac attcgttgcg tatacgctgg
    99001 ataatatcct cttgcttgaa catcactaaa gaacaaagaa gttctttgct gctcaattgt
    99061 tttttgaata tcatccggat cacaagaata cggaacaaga agctgatagc tgatcataca
    99121 accgacttgt gaacctttga taatttcatg acaagctttt gtagctaaag cacttgctat
    99181 aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt tttcttcaat
    99241 tactcctacg ctaacaaaag ggtgatgttt tacacagttg atctcattga aagtcatcca
    99301 atattttact ttatttttat agcgtttgaa tactgtttca gcgaaacgaa cgtaaaactg
    99361 aataagcttt cgattaggcc atccaccata ttctgtaacc aaatataacg gcatttcata
    99421 atgggaaaga gaaacgactg gctcaatccc atattttaga cattcgtcaa acacttgatc
    99481 ataaaaaaat aatcccgctt cattgggtgt ttcttcatca ccatgaggaa aaattcttgt
    99541 ccatgcgata gacatccgat agcatttaaa acccatttca gcaaataaac ggatgtcttc
    99601 tttaaagtga tgatagaaat catttccgtg tcttttagga taataatact tgctgttggg
    99661 atcctgcgcc gcttttactt gctgatgcgt catcatatgc cattgatctg tccatttttc
    99721 tttaggtaaa tcgctgtcga atgtatacac atcagacaca gataatcctt tttcatctac
    99781 atcccatgca ccttcacatt gattggccgc tgtcgctccg ccccataaaa atccttctgg
    99841 aaattttttc atatcgatcc ccctaaactt gctagttttc ttgtttgatt atttttctta
    99901 tgactagtaa tgatattttt gaatctacta ctatttgtaa caataaccat tgtggtcgga
    99961 tcatatccct ctttgattaa ttcttgcaaa tctacttcca tcaataaatc gcctttacgt
   100021 actttttttc catcagatac tgcactttga aagtatttgc catttaaatt cactgtatca
   100081 ataccgatat ggatcagtat ctctgctcct tcttcactga ctaccccaat tgcatgtttc
   100141 gttggaaaaa ctgttgaaac ttccccgttt acaggactgt agatttttcc gtccgttgga
   100201 atgatcgcaa acccttctcc taaagatcca ctagcaaatg ttggatcgtt tacttcagat
   100261 aaatcaatta tttcacctgc tgcaggagta cttaacacta tatcctttga ggattcttct
   100321 tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac aggaactgat catcgctaag
   100381 aaataactag caactagcca aacaaatcct tcaccaatat aaatcgggaa agacaaaata
   100441 cttcttgtag caactgccaa agcaccggct cctgcggcac cagctactcc accaaatata
   100501 gctccacata tacaagcaat gtaaaatggt cgtttatatt taagagtcac accataaaca
   100561 gcaggttcag tgattccaaa taatgctgtg ataaatgcag ataaagataa actttttact
   100621 ttcttatttt tcgttttcaa aaatacccca agtactgctc ctgcttgcgc agctacggca
   100681 gtcatacatg ctggacctag cgtatcacga ccgtagaaag aaagattgtt ggtcattact
   100741 ggtacaagtc cccaatgtaa cccaaaaatc actaacactt gccaaaatcc gccgataaca
   100801 gcaccagata aaacagggct aaagttataa acagctaaat atccagctgc aatcagctga
   100861 cttgcataat ctgcaattgg acctacaact aataaagtaa ttgggaccat cacgatgata
   100921 caaatgaatg gtgtcaataa atttcgaata ctttcgtgca agcttttgcg aatcttaggc
   100981 tcaatataag aaagaaccca tacagctaaa attgctggaa taaccgtact tgtatacctt
   101041 gctagaatca cgggcatccc tagaaatcgc aaggttatgc tatcactata tgcactgaca
   101101 atcgtaggat aaacgagagc tgctgcaata accatagcga tgaaacgatc tgtattgaac
   101161 ttttttgcag cagtatatgc caaaaatata ggcaagaaat agaaaaatgc atcagctgct
   101221 gcattcaaaa tgatataagt tcctgattca gtagttaata aattcaaact cgtacataag
   101281 gcgagtactc ctttgatcaa accacttcct gccaagacgc ctaaagtcgg ggcaaaaaca
   101341 gcggcgatca tgtctataaa agagtttaaa atgccttttt tctcagttcc tctctttcca
   101401 ctagatgcat catcttctag tttcatttgt ttggataata cgtcatacac ttcatttact
   101461 gcatttccta ctacgacttg aaacattcct ccagcttcga ctacagtgat cacaccttct
   101521 agttgctcta ccttctcctt atctgccttc ttattatctt ttagggtaaa gcgtaacctt
   101581 gttgcacaat ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg gacaatatct
   101641 ttcgctaatt gtgaatagtc catttttact tcccccttgt taattaatgc cgtagcaatc
   101701 cttcttacaa gataagtata gttcttagaa aacggtttct ccattcaatt agtacccctt
   101761 ataatagcga acgaaactgt acaatagtgt gtatggttta cttagccgag caaatcactc
   101821 actgcttgaa agaaaacaag tatatcgttt ctcttatagt caaaggagac aaaaaaaaca
   101881 cgcttgatct tcctttgctg atgtataagt ggtatctcaa gctgatactc tgtgattttt
   101941 gtttctactt ggcttttgat aagacagcac aacaaagtct cggcatttaa cggaagcata
   102001 actaggtcag aactaggaga gattagttgc tgaatggaat ctatcttttt atggattttt
   102061 atttgatttt cttctttgta gtaatgatca agcagttttg ttttcttttt ttcttcaact
   102121 atggtcagct tttcacgcaa aatctgataa atctcccaag attcaaactc atctagaaca
   102181 ctaggtagat aaatcaaatc atcccactca aaaataagct cttgctcagt agtcaaaatt
   102241 gcatcatatt ccgttatttt gtttgattga tttttgaaaa gatacactgg ttctatccgg
   102301 cattctttga tccacatgtt catactttgt tgaatctgtt tttttagtgt attcaaaata
   102361 ctaattggct ggttactaac aatcagtatc tccccttgtt ttcgaaaagg attcaacgct
   102421 tcggcaagat acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa ttgaaaatgt
   102481 attgggaaga aacgccgaat taaataaatt tctaaaggat aacgggagat gatcttttgt
   102541 acaaaatgat tcatggtatt atgtccggca tccatcctcg ttttcatttg tttgatatgg
   102601 gttagcaaaa attctgtttc atggaacaat aacgaaacag gaagatctaa aaaacgaata
   102661 atcgtttgct caaatgctct tagattcttc tctatttctt tgtttttttc atcattaatc
   102721 atcaaataat ttaactctaa tagtcgattt tcaattgctt gtttttcttg attggaaaaa
   102781 gaatagggta gttctgatga taactttttt gtgagggtct ctaccatcgg aataaataat
   102841 gtatacctgt ttgttttttc taaaagttct tctcttcgaa gaatggtcaa aacgagatac
   102901 cgagaaaatc gtatgaagtc ctctcctgaa acaatataat tatatgatat aagaacctct
   102961 tttaatgtct tttggacagt cggcatgata ttggaaaaaa ttgtactgat ttgttgaggc
   103021 aaccttgcgt gttggatcac cgtttctgtt ccaacaagag ataagaaaat tctttttgaa
   103081 ttttcttcag caatgatttt tagtcctttt gtcgacgaaa cttccagttc taatttagga
   103141 ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg atacggctgt ttttgaaaga
   103201 aagaaaatgt ctgctatctt ttgcatactg acataccctt tttgaaacaa taagtacgtc
   103261 acaatagttg aagctcggaa atccatgctg gaatagattt tatgtttcgt cacctcttgt
   103321 atgatttgct gtttagtttc agattcaatt gattctaaaa aataacctga acgtttattt
   103381 gactggataa tgattttctt ctcgtagata gcaagcatat ttttgatttc agtttggatc
   103441 gttttcttgt tggtacctaa agcttcagct aattcttcgc ttgttatcca atgattctta
   103501 tttcttaata ctacaagaat ttggagttgt cgtttattta agtccatttg cttacacctc
   103561 ctctttgtta taaacataca ggaaaaacct ctcaaatgac aatagtcact tgcagaggtt
   103621 taaaaatcat ttgaagaaag atctatattc aaggttttta ccattagact ggataatttg
   103681 tttataatat tcaaaactat cttttttcaa tctcctaaag cttccgctcc catcattttg
   103741 tctatccaca taaataaagc catatcgctt cctcatttcc cctgttcctg ccgatacgat
   103801 atcgattggt ccccaccata agtaaccaat gatttggcaa ccatctttta ttgcctcagc
   103861 aatttcctga agatgtattt ttagatactc acaacgtttt ggatcgtgga ttcgattcaa
   103921 ttcatctggt gcttcatcta gtccaatgcc attttcaact atgaataatg gtaattcata
   103981 acggtctgtc aattcattca tgacataacg aaaccctaga ggatcaattg gccaactcca
   104041 aggttcggga gatttttcct tcaaaaaggg attatcgaaa ccatttgctc cgccagtatc
   104101 tacgcgcatt tctgcttctt ttttatagac agcgctacga taataactaa atgcaatata
   104161 atctgccgtg ttagcttgca agcactgttc gtctccatct aacattactg gtgcacagtc
   104221 ttcttccttc catgtcctgt aaatataagc tggataatgc cctcggcaca tgacatcgga
   104281 gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat acattctctg gatcacagtc
   104341 atatgcataa gtcgctaatg cagaaaagct acacataata cccatcttag catttggatc
   104401 gattttcttt aataattgag tagcacgggc gttcgctaga aacatatgat gacaggcttg
   104461 ataaatcact ttttttgtta gtgtagcaat gggttcaagt ttatctctag cagggaatgt
   104521 aaccaatcca gcagctgcat aaggcatacg aaaagcatta ttgatctcat taaaggtcat
   104581 ccaatatttc actttccctt ggtagcgttc aaagacggtc tgcgcataaa attcaaaaaa
   104641 gtcgattaat tttcgatttt gccacccccc atactctcct accaatgcta agggggtttc
   104701 ataatgacta agagtaatta ctggctcgat atcatatcga tttaatgtat tgattagtct
   104761 gtcataaaat acgagaccag cctcatttgg cttctgttcg tctcctcttg gaaatattcg
   104821 tgcccatgag atgcttgttc gaaaagcttt taaacctaac tccttcagta aacgaatatc
   104881 ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa agataatgga gtgattcgtc
   104941 taaagcaatc tcccaaatct ttttttcttc attccactga atactaggat gctggttcat
   105001 gatcccgata agcgtatctg tcacattagg taatttgccg tcttccaacc atgcaccttc
   105061 acattgatta gcagctactg ctccgcccca taaaaaattt tctggaaata tttcctgatc
   105121 cataactatc tctcctcgct atcaacaatt tcttacttga agtatagctc gattcatcaa
   105181 aacaagatat tctaactatt tcggcagaag agaagaaaaa aattgaatgc tactcttcat
   105241 ttttaatatt tatatataac aaatacataa gacgatcccc gaaagaaaac actcgttaac
   105301 catctaagca aacgatttat tttacttcca acacaaatgc ttcataaggt cgtaaatcaa
   105361 ctttgccgtc tggtaactgt ttcagaggat aatttgacac aataaccctt aaagcttcac
   105421 gtggaacagt gaacgtctgc ttttcactgg acatattaca aataacgagc cattgctggt
   105481 cttcatatat gcgagaatag gcaaagacat gctcaggcgt cttttccact aattcatagt
   105541 ctccccagac gatgatgtca tgttttctac gcaaatcgat caatttcttg tacatataaa
   105601 aaacagaatc ttgatctgct aacgcttctt ctgcattgat ttctttataa tttggattga
   105661 ctcgaagcca tggaattcca gtagtaaacc cagcatgttc acgggcgttc cattgcatag
   105721 gcgtcctagc attgtctctt cctttcgcat tgattgattc caagatttcc tcttttgtaa
   105781 atccagaaga aatcctttcg ttatacatat tgattgtctc aatatctttt gcttccgcga
   105841 tatcatcgat tgggcaattc gtcatgccta actcttctcc ttgatagata taaggggtgc
   105901 ctttcatcat atggagcaag atagcaaaca tcttggcact ttcttttcga tacacatcgt
   105961 catttcccca acgagaaaca attcgcggaa gatcgtgatt attccaaaat aaactattcc
   106021 atccttcatc acctaaagat gtttgccatt tggataatac ttttttcagt tcagagatac
   106081 tcaagggttt caaatcccat ttgtctttgc cttcttgctg atctaatcct atgtgttcga
   106141 attggaagac catggataat tcatttcttt taggattgga ataaagttta gcgatttcgg
   106201 tagttgctcc ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa gttgcttgat
   106261 tcatttcctg aagatactca tgcagtttag gtccattact tgtgatttct ttatccggtt
   106321 ctttgccaat catatcgatc acgtccatac gaaagccccc aataccttta tcgatccaga
   106381 aattcatcat ttgataaatc tcttggcgaa cctgtttatt tttccaattc aaatcaggtt
   106441 gacgcttgct atacaagtgc agaaaatatt gccccgtttt ttcatccaac tgccaagcag
   106501 aaccactgaa tattgagcgc aatctattag gctcttttcc attgaccgga tcacgccaaa
   106561 tataatagtc ccgataagga ttttctttat ttttacgggc ctcaatgaac cacggatgtt
   106621 catcagaagt atgattcacc accagatcca tgatgatacg gatatttctt ttctttgctt
   106681 cttcaatcag ttcttccatt tcttccatcg taccgaattc tgccatgatt gcttgataat
   106741 tactgatatc atacccattg tcatcattcg gagactcgta tactggagaa agccaaattg
   106801 catcaatacc taaatacgcc agataatcca acctggaaat aatcccccgc aaatctccaa
   106861 taccatcttg atttgaatct tgaaaacttc ttggatagat ttgataaaca actgaatggt
   106921 gccaccattt ttgttccatg attttcctcc tataaatgga tgtttctatt ttttgcttaa
   106981 tcatttattt tagtctctct cactaaaaat tgtaacgctt tcaaatagct attgcacctt
   107041 gttatcggta acagaataaa acaaaaaaac agctgaggaa attccctcaa ctgttttttg
   107101 aaaggattga tttatgcagg gaagaatgct gatttcacaa cttctttata aacggtgttc
   107161 actcagctgt cccttggtat tagctcaaaa aacggcaaaa tatgaggaac tattttttcg
   107221 tttttttctc taatactcag gccagaacac tgatttcaca acctctttat aaacggtgtt
   107281 cactcagctg tccctcggta ttagctcaaa aaacggcaaa atataagaaa ctattttttc
   107341 gtttttttct ctaatactca ggccagaacg ctgatttcac aacctcttta tttcacattc
   107401 aatatccaca tgaatcccac aaatacaaag aacagaccat acatgattgg agatacttct
   107461 tttcctcttt tagctgctaa catggtaatt ggatagaaga taaaacctaa tgcaatccca
   107521 tctgaaatac tataagtcaa aggcatgccg atcaagatca agaatgaagg gatcgcaatc
   107581 tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc taccataatc
   107641 aatgctggtg cagttacttg tgaagtaaca actgatagta atggagaaaa gaacaagcct
   107701 aacaagaaga aaatccctgt agtaatagct gtgatacctg aacgtcctcc aacggcgata
   107761 ccagctgatg attctacaaa tgcaccaaca ggagaagtac caaataagga acccgcaagc
   107821 atagctgttg aatcagaagc taaagctttc cctacacgag gcatcttgtt gtctttcatg
   107881 aaacctgctt ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa agtcacaagc
   107941 aagaatgtca gcactacgac ccacatttga agagaattga tgtcccctac atgttttaat
   108001 gcaactaaaa atgttggttt caaacttggt gctgcggcaa caatttcaga aggcattgga
   108061 atcaaacctg tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa gatgccacct
   108121 ggtacacgcc gaacaagcaa gatagcagtt acgactaatc caaaaatcgt caaccaagta
   108181 gaaccaacgt tgagtgatcc taatcctact aatgtagatt cattcgaaac gatgatccca
   108241 ccttcgctca gaccaaggaa agcgataaat agaccgatac cgccagaaat cgcatatttc
   108301 aaatctgctg ggatcgcatc aatgatcatt tcccgcagct tgaagattgt gatcaaaata
   108361 aaaatcaaag aagcaacgaa tacgccggct aatgctgttt cccatggaat ccccattcct
   108421 aaacaaacag aataagcaaa aaaagcattg atcccaagag caggtgccgt tccaattgga
   108481 taacgggcta atacccccat taaaacacag ccgatcgcac tggctaatgc agtcgcagta
   108541 aacactgctc cttcatccat tccagaagca cctaatacag ttggatttac aaataagata
   108601 taagccattg agataaatgt tgtaaatcct gccagtacct cacgttttac cgtggtgttc
   108661 aatttttcta tttcgaagta ggaaagtatt ttttccttca tcttccatcc ccctaaatcc
   108721 gaacattata aataaagtcc gttaaaattt cgatttatta ataaaacaac gtgttttatc
   108781 atatactaaa acaaaacata agtaaacaaa aaattcggat tttaagcaaa atccgaacgt
   108841 tccatctgat taaatccctt tatctcaaag ttcatgaaca ttcactttgg attacaagaa
   108901 tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt
   108961 gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag
   109021 attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt
   109081 tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa
   109141 ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt caatcagacg
   109201 actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg aaagtcaaga
   109261 tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt
   109321 catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca
   109381 caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct
   109441 taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga
   109501 atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatca
   109561 tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc attatatcac
   109621 gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg
   109681 attgctttcc tccacaaact ttttttacaa aaaaaaaaac ggcaatgaat gccgctcttc
   109741 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   109801 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   109861 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   109921 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   109981 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   110041 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   110101 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   110161 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   110221 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   110281 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   110341 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   110401 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   110461 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   110521 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   110581 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   110641 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   110701 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   110761 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   110821 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   110881 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   110941 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   111001 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   111061 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   111121 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   111181 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   111241 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   111301 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   111361 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   111421 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   111481 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   111541 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   111601 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   111661 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   111721 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   111781 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   111841 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   111901 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   111961 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   112021 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   112081 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   112141 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   112201 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   112261 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   112321 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   112381 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   112441 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   112501 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   112561 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   112621 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   112681 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   112741 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   112801 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   112861 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   112921 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   112981 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   113041 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   113101 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   113161 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   113221 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   113281 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   113341 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   113401 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   113461 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   113521 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   113581 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   113641 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   113701 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   113761 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   113821 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   113881 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   113941 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   114001 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   114061 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   114121 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   114181 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   114241 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   114301 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   114361 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   114421 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   114481 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   114541 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   114601 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   114661 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   114721 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   114781 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   114841 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   114901 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   114961 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   115021 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   115081 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   115141 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   115201 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   115261 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   115321 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   115381 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   115441 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   115501 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   115561 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   115621 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   115681 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   115741 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   115801 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   115861 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   115921 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   115981 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   116041 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   116101 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   116161 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   116221 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   116281 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   116341 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   116401 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   116461 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   116521 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   116581 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   116641 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   116701 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   116761 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   116821 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   116881 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   116941 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   117001 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   117061 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   117121 aacacaattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   117181 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   117241 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   117301 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   117361 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   117421 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   117481 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   117541 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   117601 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   117661 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   117721 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   117781 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   117841 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   117901 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   117961 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   118021 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   118081 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   118141 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   118201 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   118261 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   118321 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   118381 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   118441 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   118501 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   118561 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   118621 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   118681 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   118741 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   118801 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   118861 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   118921 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   118981 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   119041 atagttattt ttttag
//
LOCUS       DACDLI010000002       101095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDLI010000002 DACDLI010000000
VERSION     DACDLI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 101095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.002"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..561
                     /locus_tag="IV353_000105"
     CDS             <1..561
                     /locus_tag="IV353_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008388.1"
                     /translation="IDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYG
                     KIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFF
                     YRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKIY
                     PISLKGANIHLYHIAQELHLLKKHIS"
     gene            782..2545
                     /locus_tag="IV353_000106"
     CDS             782..2545
                     /locus_tag="IV353_000106"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9008389.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2629..3087
                     /locus_tag="IV353_000107"
     CDS             2629..3087
                     /locus_tag="IV353_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9008390.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3196..3879
                     /locus_tag="IV353_000108"
     CDS             3196..3879
                     /locus_tag="IV353_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9008391.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4026..4640
                     /locus_tag="IV353_000109"
     CDS             4026..4640
                     /locus_tag="IV353_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9008392.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4656..4862
                     /locus_tag="IV353_000110"
     CDS             4656..4862
                     /locus_tag="IV353_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008393.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            5403..6533
                     /locus_tag="IV353_000111"
     CDS             5403..6533
                     /locus_tag="IV353_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9008394.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            6625..7380
                     /locus_tag="IV353_000112"
     CDS             6625..7380
                     /locus_tag="IV353_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9008395.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            7438..9477
                     /locus_tag="IV353_000113"
     CDS             7438..9477
                     /locus_tag="IV353_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9008396.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(9586..10737)
                     /locus_tag="IV353_000114"
     CDS             complement(9586..10737)
                     /locus_tag="IV353_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9008397.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(10891..11421)
                     /locus_tag="IV353_000115"
     CDS             complement(10891..11421)
                     /locus_tag="IV353_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008398.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(11520..11870)
                     /locus_tag="IV353_000116"
     CDS             complement(11520..11870)
                     /locus_tag="IV353_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9008399.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(12027..13556)
                     /gene="ahpF"
                     /locus_tag="IV353_000117"
     CDS             complement(12027..13556)
                     /gene="ahpF"
                     /locus_tag="IV353_000117"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9008400.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(13566..14129)
                     /gene="ahpC"
                     /locus_tag="IV353_000118"
     CDS             complement(13566..14129)
                     /gene="ahpC"
                     /locus_tag="IV353_000118"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9008401.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(14273..14935)
                     /locus_tag="IV353_000119"
     CDS             complement(14273..14935)
                     /locus_tag="IV353_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9008402.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            15119..16765
                     /locus_tag="IV353_000120"
     CDS             15119..16765
                     /locus_tag="IV353_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9008403.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            16756..17838
                     /gene="uxuA"
                     /locus_tag="IV353_000121"
     CDS             16756..17838
                     /gene="uxuA"
                     /locus_tag="IV353_000121"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9008404.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(17946..19499)
                     /locus_tag="IV353_000122"
                     /pseudo
     CDS             complement(17946..19499)
                     /locus_tag="IV353_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            19647..20788
                     /locus_tag="IV353_000123"
                     /pseudo
     CDS             19647..20788
                     /locus_tag="IV353_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(20914..21993)
                     /locus_tag="IV353_000124"
     CDS             complement(20914..21993)
                     /locus_tag="IV353_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9008405.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(22096..22536)
                     /locus_tag="IV353_000125"
     CDS             complement(22096..22536)
                     /locus_tag="IV353_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9008406.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(22593..23003)
                     /locus_tag="IV353_000126"
     CDS             complement(22593..23003)
                     /locus_tag="IV353_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9008407.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(23183..23617)
                     /locus_tag="IV353_000127"
     CDS             complement(23183..23617)
                     /locus_tag="IV353_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9008408.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(23717..24565)
                     /locus_tag="IV353_000128"
     CDS             complement(23717..24565)
                     /locus_tag="IV353_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9008409.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            24954..26720
                     /locus_tag="IV353_000129"
     CDS             24954..26720
                     /locus_tag="IV353_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9008410.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            26822..28081
                     /locus_tag="IV353_000130"
     CDS             26822..28081
                     /locus_tag="IV353_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9008411.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            28228..29529
                     /locus_tag="IV353_000131"
     CDS             28228..29529
                     /locus_tag="IV353_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9008412.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            29532..30383
                     /locus_tag="IV353_000132"
     CDS             29532..30383
                     /locus_tag="IV353_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9008413.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            30396..31211
                     /locus_tag="IV353_000133"
     CDS             30396..31211
                     /locus_tag="IV353_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9008414.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            31362..32528
                     /locus_tag="IV353_000134"
     CDS             31362..32528
                     /locus_tag="IV353_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9008415.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            32758..33405
                     /locus_tag="IV353_000135"
     CDS             32758..33405
                     /locus_tag="IV353_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9008416.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            33489..34433
                     /locus_tag="IV353_000136"
     CDS             33489..34433
                     /locus_tag="IV353_000136"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9008417.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            34568..36019
                     /locus_tag="IV353_000137"
     CDS             34568..36019
                     /locus_tag="IV353_000137"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9008418.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            36040..36876
                     /locus_tag="IV353_000138"
     CDS             36040..36876
                     /locus_tag="IV353_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9008419.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            36876..37535
                     /locus_tag="IV353_000139"
     CDS             36876..37535
                     /locus_tag="IV353_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9008420.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            37661..38350
                     /locus_tag="IV353_000140"
     CDS             37661..38350
                     /locus_tag="IV353_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008421.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            38356..39312
                     /locus_tag="IV353_000141"
     CDS             38356..39312
                     /locus_tag="IV353_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9008422.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            39356..39868
                     /locus_tag="IV353_000142"
     CDS             39356..39868
                     /locus_tag="IV353_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9008423.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(40124..40672)
                     /locus_tag="IV353_000143"
     CDS             complement(40124..40672)
                     /locus_tag="IV353_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9008424.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(40761..41942)
                     /locus_tag="IV353_000144"
     CDS             complement(40761..41942)
                     /locus_tag="IV353_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9008425.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(42178..43686)
                     /locus_tag="IV353_000145"
     CDS             complement(42178..43686)
                     /locus_tag="IV353_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9008426.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            43845..44036
                     /locus_tag="IV353_000146"
     CDS             43845..44036
                     /locus_tag="IV353_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008427.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            44239..45156
                     /locus_tag="IV353_000147"
     CDS             44239..45156
                     /locus_tag="IV353_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9008428.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            45149..46222
                     /locus_tag="IV353_000148"
     CDS             45149..46222
                     /locus_tag="IV353_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008429.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            46370..46888
                     /locus_tag="IV353_000149"
     CDS             46370..46888
                     /locus_tag="IV353_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008430.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            46869..47261
                     /locus_tag="IV353_000150"
     CDS             46869..47261
                     /locus_tag="IV353_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9008431.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            47337..48272
                     /locus_tag="IV353_000151"
     CDS             47337..48272
                     /locus_tag="IV353_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9008432.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            48405..49184
                     /locus_tag="IV353_000152"
     CDS             48405..49184
                     /locus_tag="IV353_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9008433.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            49177..49494
                     /locus_tag="IV353_000153"
     CDS             49177..49494
                     /locus_tag="IV353_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9008434.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            49738..51348
                     /locus_tag="IV353_000154"
     CDS             49738..51348
                     /locus_tag="IV353_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9008435.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            51505..52353
                     /locus_tag="IV353_000155"
     CDS             51505..52353
                     /locus_tag="IV353_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9008436.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            52584..53063
                     /locus_tag="IV353_000156"
     CDS             52584..53063
                     /locus_tag="IV353_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008437.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            53096..55324
                     /locus_tag="IV353_000157"
     CDS             53096..55324
                     /locus_tag="IV353_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9008438.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            55453..57096
                     /locus_tag="IV353_000158"
     CDS             55453..57096
                     /locus_tag="IV353_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9008439.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(57169..57714)
                     /locus_tag="IV353_000159"
     CDS             complement(57169..57714)
                     /locus_tag="IV353_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9008440.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(57737..58222)
                     /locus_tag="IV353_000160"
     CDS             complement(57737..58222)
                     /locus_tag="IV353_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9008441.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(58212..59024)
                     /locus_tag="IV353_000161"
     CDS             complement(58212..59024)
                     /locus_tag="IV353_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9008442.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(59163..60695)
                     /locus_tag="IV353_000162"
     CDS             complement(59163..60695)
                     /locus_tag="IV353_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9008443.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIISLSPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(60804..61835)
                     /locus_tag="IV353_000163"
     CDS             complement(60804..61835)
                     /locus_tag="IV353_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9008444.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            62559..63545
                     /locus_tag="IV353_000164"
     CDS             62559..63545
                     /locus_tag="IV353_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9008445.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            complement(63694..65034)
                     /locus_tag="IV353_000165"
     CDS             complement(63694..65034)
                     /locus_tag="IV353_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9008446.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(65039..65713)
                     /locus_tag="IV353_000166"
     CDS             complement(65039..65713)
                     /locus_tag="IV353_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9008447.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQPPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            65935..67008
                     /locus_tag="IV353_000167"
     CDS             65935..67008
                     /locus_tag="IV353_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9008448.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            67014..67700
                     /locus_tag="IV353_000168"
     CDS             67014..67700
                     /locus_tag="IV353_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008449.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            67708..68355
                     /locus_tag="IV353_000169"
     CDS             67708..68355
                     /locus_tag="IV353_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9008450.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(68428..69819)
                     /locus_tag="IV353_000170"
     CDS             complement(68428..69819)
                     /locus_tag="IV353_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9008451.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            70076..73465
                     /locus_tag="IV353_000171"
     CDS             70076..73465
                     /locus_tag="IV353_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9008452.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDCVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            73468..74889
                     /locus_tag="IV353_000172"
     CDS             73468..74889
                     /locus_tag="IV353_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9008453.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            74886..76763
                     /locus_tag="IV353_000173"
     CDS             74886..76763
                     /locus_tag="IV353_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9008454.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            76855..77697
                     /locus_tag="IV353_000174"
     CDS             76855..77697
                     /locus_tag="IV353_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9008455.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(77849..78724)
                     /locus_tag="IV353_000175"
     CDS             complement(77849..78724)
                     /locus_tag="IV353_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9008456.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            78931..79962
                     /locus_tag="IV353_000176"
     CDS             78931..79962
                     /locus_tag="IV353_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9008457.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            80051..80356
                     /locus_tag="IV353_000177"
     CDS             80051..80356
                     /locus_tag="IV353_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9008458.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            80535..82550
                     /locus_tag="IV353_000178"
     CDS             80535..82550
                     /locus_tag="IV353_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9008459.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            82552..82836
                     /locus_tag="IV353_000179"
     CDS             82552..82836
                     /locus_tag="IV353_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9008460.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            82862..84235
                     /locus_tag="IV353_000180"
     CDS             82862..84235
                     /locus_tag="IV353_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9008461.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(84318..86333)
                     /locus_tag="IV353_000181"
     CDS             complement(84318..86333)
                     /locus_tag="IV353_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAP9008462.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(86355..87569)
                     /locus_tag="IV353_000182"
     CDS             complement(86355..87569)
                     /locus_tag="IV353_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9008463.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            87790..88734
                     /locus_tag="IV353_000183"
     CDS             87790..88734
                     /locus_tag="IV353_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9008464.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            89030..89974
                     /locus_tag="IV353_000184"
     CDS             89030..89974
                     /locus_tag="IV353_000184"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9008465.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(90641..91039)
                     /locus_tag="IV353_000185"
     CDS             complement(90641..91039)
                     /locus_tag="IV353_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9008466.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(91036..91272)
                     /locus_tag="IV353_000186"
     CDS             complement(91036..91272)
                     /locus_tag="IV353_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9008467.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(91432..91749)
                     /locus_tag="IV353_000187"
     CDS             complement(91432..91749)
                     /locus_tag="IV353_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008468.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            complement(91887..94115)
                     /gene="topB"
                     /locus_tag="IV353_000188"
     CDS             complement(91887..94115)
                     /gene="topB"
                     /locus_tag="IV353_000188"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9008469.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(94289..94699)
                     /locus_tag="IV353_000189"
     CDS             complement(94289..94699)
                     /locus_tag="IV353_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9008470.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(94816..95979)
                     /locus_tag="IV353_000190"
     CDS             complement(94816..95979)
                     /locus_tag="IV353_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9008471.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            complement(96157..97011)
                     /gene="aroE"
                     /locus_tag="IV353_000191"
     CDS             complement(96157..97011)
                     /gene="aroE"
                     /locus_tag="IV353_000191"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9008472.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            complement(97290..98060)
                     /locus_tag="IV353_000192"
     CDS             complement(97290..98060)
                     /locus_tag="IV353_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9008473.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            complement(98063..98944)
                     /locus_tag="IV353_000193"
     CDS             complement(98063..98944)
                     /locus_tag="IV353_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9008474.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            complement(98941..99990)
                     /locus_tag="IV353_000194"
     CDS             complement(98941..99990)
                     /locus_tag="IV353_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9008475.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            complement(100440..101063)
                     /locus_tag="IV353_000195"
     CDS             complement(100440..101063)
                     /locus_tag="IV353_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008476.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT        33160 a        18482 c        19141 g        30312 t
ORIGIN      
        1 atcgacgatg cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg
       61 tgggcagatg gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa
      121 cttctgtacg gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa
      181 ctaagatgga atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt
      241 gaacaagcgg ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa
      301 tttttttatc gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct
      361 attttatttc gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg
      421 gttttgtgcg atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat
      481 ccaatctcat taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta
      541 ttaaaaaaac atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa
      601 ttgtctgtag aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa
      661 gctttctctg aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat
      721 agataaagtg taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat
      781 tatgagtaca gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt
      841 atctgttgag gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga
      901 cagcgatgta ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt
      961 tcattatgta tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta
     1021 tggtcataag cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt
     1081 gagcggatat tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc
     1141 tacatctatc agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg
     1201 aaatgtggta gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt
     1261 gaacaatggt ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc
     1321 tattgatgag aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg
     1381 taaaagcagc aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg
     1441 aaacgatctg gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat
     1501 tgtacttcac gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat
     1561 gaaatatcat tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc
     1621 tggagaaaca tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac
     1681 agctcctatt gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca
     1741 agaaaaacat ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt
     1801 cgcttctgct ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt
     1861 gcaacgggca tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat
     1921 tttagtcggt ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat
     1981 tccttactta tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat
     2041 gatctctatg ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc
     2101 tacacatggt gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca
     2161 aacagtaaat aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg
     2221 aaaagaaaca gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg
     2281 aacgatcaca gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt
     2341 catcacatta gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta
     2401 tggtgattct tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt
     2461 tcctgtaggg cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag
     2521 tctgcgtgtc ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac
     2581 tagtatctgg atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa
     2641 gtagaagaat atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta
     2701 ggtttctttt catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta
     2761 aaaaagataa agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct
     2821 ttaccaaaag ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc
     2881 atcaaaaata taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg
     2941 aaagaaggag aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt
     3001 aaacatgctt ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac
     3061 gaagaatttg aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta
     3121 ttgtctcctg aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt
     3181 actggaggag atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt
     3241 caagctttgg aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct
     3301 gcaaaagaag gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc
     3361 aagcgaatcg ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa
     3421 gtgtttatta ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc
     3481 attgcactgg atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc
     3541 caaatgattc acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg
     3601 cttcaagcgc aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa
     3661 tattcacgaa agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc
     3721 gatatcccag taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat
     3781 gaattaggtg ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact
     3841 aaaagatatg tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt
     3901 cttgacaaaa aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag
     3961 cattactttt cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg
     4021 aaaccatgtt tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga
     4081 ttggttcagt catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag
     4141 tgaatatcgc acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct
     4201 tggcgatcca gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa
     4261 gagaagctgt tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag
     4321 ccaatctgcc taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag
     4381 taatcatttc agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg
     4441 aacatgtcga gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac
     4501 agaaatatca tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa
     4561 aattgaatgc tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca
     4621 cgattgcacg tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat
     4681 cgacagattc aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc
     4741 agtacttaga agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg
     4801 gtaaggaaat agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaacaaat
     4861 aaatatttat tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat
     4921 caaagaatcc tggtgcaact gaaagaattt gcaacgggat tctttcaact tatttgttat
     4981 ttttttctaa aaaatattaa agattgttaa atttcactat ttcatttcaa attccattgc
     5041 ctaatcatta ggaaaagtct atcatgaact cgtaaataga atatagtaat aaccttgtta
     5101 ggtgaggctc ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt
     5161 aaaacagaaa ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg
     5221 tatagtgcta aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt
     5281 ttctgacctt ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc
     5341 acctgacaag tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga
     5401 ttttgggtcc ttatttgact ttgtccatga tcggtctttt agaagtatac agtgccagtt
     5461 cttatcgttt attgcaagcc gatgaaaata caaaaagtct attattgcgt caactaattt
     5521 tcatattttt gagttggagc gtgatcttct tagctcgttc agtcaaacta cactatttac
     5581 ttcaccctaa aatagcagga tacggtttag ccttatcgat tttcttttta gtattagtaa
     5641 gaatagggat attcggtgtc actgtcaacg gcgcacaacg ttggatctct ctgtttggca
     5701 ttcaattcca gccttctgaa ctggcaaatc tttttttgat tttttattta agctggtttt
     5761 ttcgtgacgg aaatagtagc ccaaaagatc taaaaaaacc attcctgatt acagtaggta
     5821 taacttttct gattttattt cagccaaaga ttgctggagc attgatgatc ctttcgattg
     5881 cgtgggtcat attttgggca gcggcggttc catttaaaaa agggatctat ctaatcgtta
     5941 ctttttctgc attgctgatt ggagcagcag gcggggtatt atatttagga aataaaggtt
     6001 ggcttccaca gatgtttaat catgcttatg aaaggatcgc gactttaaga gattctttta
     6061 tcgatagtca tggtgcgggc tatcaaatga cccattcgtt ttatgcacta tataatggtg
     6121 gcatatttgg gcggggatta ggaaatagta tcacaaaaaa agggtatctt ccagagacag
     6181 aaacagattt tattttttcg attattacag aagagcttgg gctaatcggt gcattgtgtg
     6241 tgcttttctt attgttctca ttatgcatga gaatcttttg tttaagcagt cgctgtaaaa
     6301 atcagcaagc tggattgttt cttttaggat tcggaacttt gctattcgtt cagacgataa
     6361 tgaacgtggg aagtatcgcg gggctgatgc caatgactgg tgtgccattg ccatttgtta
     6421 gctacggagg aacaagttat ctgatccttt ctttaggaat cggaatcacg ttgaatattt
     6481 cttcaaagat acaggccgaa gagttggcgc tctatcgacc tgaaaaacaa taaagagaaa
     6541 ctgagaaact ctcagtttga attatcataa agtacaatct tttatttttc aagcgagagg
     6601 ttcttttaga aagaggaatt cgcaatgaaa gtatctcaaa aaatcatatt atctattttg
     6661 ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg attttcgtct
     6721 gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac cacactaaat
     6781 gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc ggattcactt
     6841 ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat catgagagat
     6901 tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta ctcttatggg
     6961 ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc tccctattat
     7021 gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc tggtggagtc
     7081 aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa aggagagcaa
     7141 gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa agaaggagac
     7201 ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca gctgaaagac
     7261 gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag tatccaaacg
     7321 aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga catatgttag
     7381 aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga ggaaaaaatg
     7441 aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg cgcagtgatc
     7501 ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga aacccaagca
     7561 gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa gggagattat
     7621 aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt atctgaaaaa
     7681 gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg acttcagata
     7741 tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata taaagcaaag
     7801 atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt agacagaaat
     7861 gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat ggaaggaaat
     7921 gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga tcgaaatggt
     7981 gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag taaacttgga
     8041 gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga cttaacagaa
     8101 gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt tgtcccattg
     8161 aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca agaagtagac
     8221 ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt gggagatatc
     8281 acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat cggtcgctca
     8341 ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa attaagcatt
     8401 acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca aaatggcaaa
     8461 gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag tctaggagga
     8521 aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc gcttgctagc
     8581 tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga ttacaaagct
     8641 tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta tgctcctgga
     8701 tctacgttta aaatgattac agcagcaatc ggtctcgaca acggcactat cgatccaaat
     8761 gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg atcttatcaa
     8821 gcaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt gatctattcc
     8881 gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt tcgtacaggc
     8941 ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa tccagcacaa
     9001 atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg atatggacag
     9061 ggcgaacttc taattactcc tatccagcaa gcagcaatgt attctgtttt taccaacaat
     9121 ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa gaagaatgtc
     9181 atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt gcaagatgta
     9241 aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa aactggtaca
     9301 gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt gtttgctttc
     9361 aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga agatgatgat
     9421 tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta tcaataaaaa
     9481 attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct tttttcaaat
     9541 aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca cttcataaac
     9601 tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata aaatcacgat
     9661 aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa tttgtaaaat
     9721 gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg aactatgttg
     9781 agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca aaatcgctac
     9841 aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat caacttctaa
     9901 tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct ctacattgtg
     9961 gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa aagcaccaat
    10021 gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa taactagcga
    10081 tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta ggaaaggcgc
    10141 aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa tcaaaacaag
    10201 cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga aacttagaac
    10261 taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc cttcttttgt
    10321 attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag ttgccgcaag
    10381 aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc ctgtcaacca
    10441 gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag gtcggaaata
    10501 ttttttcaaa agagataaat cactttccag accagctaaa aacattaata agatcacacc
    10561 aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc cagcttggcc
    10621 tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc tccgcgagat
    10681 atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa attccattct
    10741 catcattcct ttgtataaaa tatagcccga acttagcata acatacatta ttgaaaaaat
    10801 aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat tgttggtttt
    10861 tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc ttattctgat
    10921 gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata tttggaatag
    10981 aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca acaacagaat
    11041 actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca actggaacaa
    11101 gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga ttgcaggtgc
    11161 aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca gaccgatact
    11221 ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca acccaagcaa
    11281 aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag ggtttagcac
    11341 aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt tttgaatatt
    11401 ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa cgtgaactag
    11461 tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct ctggaaaaat
    11521 caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat cattttatct
    11581 aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac accacaaaca
    11641 acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa aaattgttcg
    11701 aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc cgtcagccaa
    11761 caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt tgctacatac
    11821 ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat aatcagtcac
    11881 ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg
    11941 attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcacaatcct tttttccgct
    12001 ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa tgctcctaaa
    12061 gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact atctgtacaa
    12121 tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt cactatttca
    12181 tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag taagccgact
    12241 agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg atcgatatag
    12301 gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg tttgataaca
    12361 gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt taattctggt
    12421 aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc tgcttcaaca
    12481 ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg gccgtcacag
    12541 tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac attgatagaa
    12601 cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat accgttagct
    12661 aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg attcatcata
    12721 atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt tggaccttct
    12781 gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg tccgccgaaa
    12841 gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc tgcactacta
    12901 gctgctggtc caccgccaat cactaataca tcataaggtt ctttttcaaa caactcttct
    12961 tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg tccgctacta
    13021 aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc atcgatttct
    13081 ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa tacagctaat
    13141 atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac ataactgtca
    13201 aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact gattttcggt
    13261 gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg accgaggggg
    13321 ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa actaggtgtg
    13381 cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat ttccttcgta
    13441 aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa aacaatagga
    13501 gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc taacatatcc
    13561 accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc actttctttc
    13621 cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc gcgaatctta
    13681 tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat agcttgaacg
    13741 attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc ttcatccaaa
    13801 acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta ttcgatttta
    13861 ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc ggtagagcaa
    13921 gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa gtcttctaat
    13981 tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact ccagttcccc
    14041 ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg tgcattaaat
    14101 tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat tatttattta
    14161 taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat ttaataaaga
    14221 ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa tgctactgct
    14281 ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc atgtgtaagt
    14341 gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc ttgagtaaat
    14401 cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact cgtaaccagc
    14461 ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct tttcttcctg
    14521 caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg cgttcgtcat
    14581 ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt cgtgtttgtg
    14641 tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct cggacaaagc
    14701 gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt tgaggtatcg
    14761 tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga gtccggccga
    14821 tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct aagtcagcat
    14881 agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt ttcatcttta
    14941 ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa aacaatctgg
    15001 tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac atagcgattt
    15061 ttattgcata aaattcactt gaatacaaaa actaatatgt tagtttagga ggggaaaaat
    15121 gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag aagtagaaat
    15181 acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg tctggatcca
    15241 tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac gtttagcgga
    15301 tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga
    15361 aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa gcagtggaga
    15421 gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc cagattgttc
    15481 tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat tagttacttt
    15541 tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata taggaccgat
    15601 tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta ttcttgctgt
    15661 gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct ctacagataa
    15721 tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa aagaatggca
    15781 aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag aaattgttgc
    15841 gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag ttagcaaaat
    15901 gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag gaaataatat
    15961 tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc attttcccaa
    16021 tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa cggtagataa
    16081 agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt tagctattat
    16141 gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag atttatcgct
    16201 gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc caggtatcat
    16261 tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc caaatattcc
    16321 taatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc gttatggtga
    16381 gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg tattcatacc
    16441 attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag gaaaaccatt
    16501 catcccgagt ccagacccac tattgacaga ccttcagcat aagctttccg atattcaact
    16561 gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt caaataaaga
    16621 aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag aaatgttcat
    16681 caagatggta gcaggaccaa atgccgtacg aaaaacatta caggattact tacaagaaca
    16741 tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga agaaaagatt
    16801 accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac attgatggat
    16861 ataccagtag gagaggtatg gcctagggag aggatcaaag aactaaaaga agaaattgag
    16921 tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt
    16981 ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg aaacttggca
    17041 gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg ggtgaaatct
    17101 gaccttgact accgcttaga agacggctca tccacgttag cttttatatc tgatgatatt
    17161 ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga atttggattg
    17221 ccgggatggg aacctgagcg cttagctcct atcaaatcct tgcttgaagc ctatgcatca
    17281 gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat catcccaaca
    17341 tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata ctcgattttt
    17401 ggattagctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgtcg catagtagac
    17461 agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc agaaaatgat
    17521 gtatatggga ttttagcaga attttgccaa agagatcgaa tcccatttgc acatgttcga
    17581 aatattaaat ttttagctaa tcgagatttc tatgaggcac cacataaatc cgaatacggt
    17641 tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa cggatacatc
    17701 cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata cggcttatat
    17761 gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga aaaaggaaac
    17821 aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa gaaggatgtt
    17881 caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta tgtatcttat
    17941 ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc ttcttctttt
    18001 aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc tggtgtgatg
    18061 attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa agggcagtcg
    18121 ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga ttcatttgaa
    18181 aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg cgtatagttc
    18241 acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac taggtcttca
    18301 accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg attttgttct
    18361 ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaatac gctatgttct
    18421 tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag aaatccatta
    18481 taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc aggtataaac
    18541 aaatcaaata cagtacaaag ggactccacc atttctcttg aataaagaac atgaggaacg
    18601 tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc gattttcgtg
    18661 atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat tggcagatta
    18721 ggtcgcaaaa tatctacaga gagatcaata aaaaagtcaa tatattcata ctctgtgaat
    18781 tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat ttttaataga
    18841 aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt tttaaaaaaa
    18901 gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt agcagtactt
    18961 gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc ttttccagaa
    19021 aaagactttc tgccattatt agaaaaggag atagacaaac cgtactatgc taaaaatgca
    19081 ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg ctgactgaaa
    19141 ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata actgactgat
    19201 tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat tccttcaaaa
    19261 ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt gagacgctca
    19321 aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat atggaaatga
    19381 tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag aaaaatatct
    19441 aattttgtat tgcttttttt cttcaagata aacgttttaa tcgcttcttg agtttgcatg
    19501 aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt tttttcataa
    19561 atttagacaa aaaaacagta aacatatagg tagaatctct actatcttat ttcttgcata
    19621 caaaaaaatc aaggagtgag agagttatga gatattcaac catcttaata tcaacatttg
    19681 cagccttaag cttatcgctt actataaatc agacagtcca cacagaggag acagaaacag
    19741 ccaaatggac agaaaccaag tgggccaacg tcaatcgaat tacctttcag gatgaatttt
    19801 catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca aacagcgacc atcaaagcaa
    19861 aaattgacta ttcgggagac ttagcaccta tactacaatc agctgttaca tttgagaatg
    19921 ttgatcccac tttgtcgatt ggctatgacg agaacacttt gactatccaa cacaagcaag
    19981 cgatatttac cttcaccgtt acattaaatc aggacttgtt aaaaccagcg ctttttactg
    20041 tcaaggtatc agatggacta ccgaacgatc atcatttaac gccgtatagt cagcggcaga
    20101 caattgagca agattcagcc atcgactcag gaggagatct tgttgaagaa ccaacagata
    20161 aaccagaaaa tgaaaataag ccagaagtac cgcctacaga aaatccagac ggagaacaga
    20221 aaccggaaat agaacctgga gaagaacccg atacagaaac tcaaccagag ccagacaata
    20281 aatcaaaacc tgaaataacc cctggagaaa aaccagatgt aactccagaa ccagatacgg
    20341 attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc
    20401 cagaaaaacc agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg
    20461 acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa
    20521 aaaaagagag taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata
    20581 aaaaatcacg aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata
    20641 caacctttgc tgtcctgaaa cgaaaaataa acagctacca aaatccggag agtcacaaaa
    20701 caaagttata ctgtggagcg gaatcattct attaagtata gcaacaatgc tttctgcaaa
    20761 aagatttaaa caaaacagat ctctctaaca tttgaataat gtctgtgaac gcatttttca
    20821 aataaaaaga aatatccaga aaatgacccc aaaataccag attgaaatct gatatttggg
    20881 gtcgttcata ctttatcttt cttcatattt attttaaggg atatctgttt cttttggaag
    20941 atggttcggg atctttttca ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc
    21001 gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca agtaagacca caaatgtata
    21061 aaaaatcggc agttctgttt tgctggacat caacttcgga ttcagcacat aagactcaat
    21121 tagatggaca atcaaaatcg taatcaggat ataaacaaca tctcggatgc ctccttggga
    21181 ataagctatg aaagatagag gaatacaaga aatgataaca cctgctactg ggatcagact
    21241 taaaatgaaa atcatgatcg ctaaactcag cagctgatga aagccaaata cagccaaggc
    21301 gattgtagtg agcaacgtat ttaaaagagc gacaacaaat tgagcttcta ataccaaacc
    21361 aaatgtattg acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc
    21421 actttttaaa aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa
    21481 acttaagatg aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc
    21541 aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt
    21601 atggatatat tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aattaataac
    21661 ggactggatc atgtcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag
    21721 atagataagg taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg
    21781 gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag
    21841 caaaatggtt gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac
    21901 agtgagacgg cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac
    21961 ctccaaataa caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa
    22021 gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc
    22081 gttcatctag cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg
    22141 ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt
    22201 ttgtggaata ccttctgctt gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt
    22261 tacgattctt tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt
    22321 ttttagtaag ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac
    22381 tgaagtcttt ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt
    22441 cagcggcttg aggaggggag tgtagtgtct aatgatttct ttagaggcgg catataaagg
    22501 aaaacatact tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta
    22561 tagtgtatca tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact
    22621 tctatatttc cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat
    22681 tcttgcgttt cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca
    22741 aagctcgttt cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag
    22801 atattttttt gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct
    22861 gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc
    22921 aattcgaatg tacgatcggg attatgcact tctccagagc gtccgcctgt gttgatcatt
    22981 ttcgtagaat acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa
    23041 ttttacacaa tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa
    23101 agcacatgaa acagacgcgc tttctcgaat agcaaacata tacaaaaaat atcagagaca
    23161 aaaaataatc tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa
    23221 aaattacttc acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca
    23281 ttgagttagt cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat
    23341 ttcgtaatgc tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg
    23401 tatttggatg ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat
    23461 ggaatctcgt accatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg
    23521 cctcaatcag ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat
    23581 catttaatac aaaatcgatt tccgttcctt tatgcataaa gatctcctct ccttttcagt
    23641 cgaacagtta ctagattctt ttattatacc agctaaagaa aacgcattca aaaaaatagc
    23701 ggatttactt accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg
    23761 ctacgctgct gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc
    23821 gggaaaattc atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc
    23881 cgccttttgc ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga
    23941 tgtcttccaa ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt
    24001 cgatataagt catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt
    24061 ctattccaaa aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa
    24121 tcgaagtatt caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc
    24181 tagaagagtt gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa
    24241 tgacagacaa caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag
    24301 gaaaaaccaa gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag
    24361 ctagtaccaa cataacgata ttcaacacta aaataactat agctcggtca aagcctaaag
    24421 cagcttccag taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta
    24481 aaaacatatt gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc
    24541 atataaattg tctcgtttct ttcatttatt cacttcctta attcattaat aatactctta
    24601 tcttattata acttactttt caccttaagt taactttaat taataaaaaa attaggctag
    24661 tctaaattat tatgtttcat tcttctagaa tatacttcta cgccaaaaac tgttaaaaaa
    24721 cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag
    24781 tacaggaagt agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa
    24841 aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg
    24901 cttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata
    24961 cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat
    25021 ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag
    25081 atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc
    25141 taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac
    25201 agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa
    25261 tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt
    25321 ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa
    25381 tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt
    25441 ggggaagtaa agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg
    25501 atcatttgga ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta
    25561 aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg
    25621 gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca
    25681 tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca
    25741 aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt
    25801 cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt
    25861 ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg
    25921 atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta
    25981 atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag
    26041 acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat
    26101 tccatgcagt gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga
    26161 aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc
    26221 aggtgaacga aggaacattc aacttattag attctcatga tactgccagg atcttgaccc
    26281 tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag
    26341 ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt
    26401 gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa
    26461 aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt
    26521 ggctaatagt aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga
    26581 tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg
    26641 tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg
    26701 gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga
    26761 agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa
    26821 gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg
    26881 cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa
    26941 aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac
    27001 tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt
    27061 ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata
    27121 tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga
    27181 agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga
    27241 acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc
    27301 atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga
    27361 agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt
    27421 aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg
    27481 tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt
    27541 ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat
    27601 gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa
    27661 caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc
    27721 gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca
    27781 aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa
    27841 tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac
    27901 aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg
    27961 cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc
    28021 aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg
    28081 attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg
    28141 caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg
    28201 tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa
    28261 aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct
    28321 tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc
    28381 ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga agatcattca
    28441 ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt
    28501 tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa
    28561 atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta
    28621 ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca
    28681 ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag tttaattgat
    28741 tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt
    28801 tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc
    28861 cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc
    28921 ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg
    28981 aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag
    29041 cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta
    29101 gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga
    29161 atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca
    29221 tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt
    29281 attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggc
    29341 ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat
    29401 aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc
    29461 tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg
    29521 ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact
    29581 tttttactta cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca
    29641 cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg
    29701 actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata
    29761 aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg
    29821 cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt
    29881 tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct
    29941 tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc
    30001 caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat
    30061 ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg
    30121 tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca
    30181 tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa
    30241 catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga
    30301 ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga
    30361 catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt
    30421 tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta
    30481 aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt
    30541 atgccaatca gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg
    30601 atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat
    30661 cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac
    30721 aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa
    30781 aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt
    30841 ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc
    30901 gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga
    30961 gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat
    31021 ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca
    31081 tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa
    31141 caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc
    31201 gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata
    31261 aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt
    31321 agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat
    31381 acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta
    31441 caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat atccatgaag
    31501 aaatcccaat gaaagagatt acaagtaacg catctttggc actaggagaa gcctatatgg
    31561 atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag
    31621 caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata
    31681 cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata
    31741 aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata
    31801 cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag
    31861 aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca
    31921 aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta
    31981 ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc
    32041 gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag
    32101 gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa
    32161 cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata
    32221 aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa
    32281 aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag
    32341 aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt
    32401 ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta
    32461 atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc
    32521 gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg
    32581 atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag cttagtttct
    32641 ttttctttat attttcataa taaaagtttg gttctttaat ttttaaacat gacagaaaat
    32701 tttttcttcg ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg
    32761 aaaaaaactg ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca
    32821 aataccaatc aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag
    32881 gtatcggact cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt
    32941 gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa
    33001 gataaatacc cagatactgc aattactagt ttggaattag atagtgattg gggatcttac
    33061 ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa
    33121 aatgaaaaaa tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa
    33181 aagaaaaaag aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa
    33241 atcgccgtcg acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta
    33301 ggtactactt attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc
    33361 aatagccaaa caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa
    33421 acaagagttg atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag
    33481 cgatttactt gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga
    33541 atgcatctgg tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag
    33601 caggagcatt tatcactaag agctgtacga tcaatgaacg aaaaggaaat cccgaaccac
    33661 gctattttga tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt
    33721 cttattatct agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt
    33781 tatttttttc cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag
    33841 aaaaaagtgg gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa
    33901 aaccacaact agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca
    33961 tcttttctaa gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc
    34021 agatggctga tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg
    34081 gcaatggttt gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt
    34141 tcggcggcat tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt
    34201 atacacgttt gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag
    34261 atgcatttga acatctgcta tgtggcgcaa gcatgttaca gattggtacc gagcttcata
    34321 aagaagggcc ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa
    34381 aaggatatac gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag
    34441 acgaaggtca tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg
    34501 tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga
    34561 ggataatatg aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt
    34621 tcaagaacta gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc
    34681 gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga
    34741 aaaagggcca ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg
    34801 atggctagct acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa
    34861 ttatgtgcgg caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga
    34921 gtttggtttc aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa
    34981 ccttgaatgt acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat
    35041 cgttccttta gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg
    35101 cggattgatt gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag
    35161 aggatggcaa ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc
    35221 tttattttat gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga
    35281 gtggcaaaca catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat
    35341 ccactcacag attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg
    35401 aaaagaaggg catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac
    35461 gtcttattat cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg
    35521 catccagcga gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata
    35581 tggtcagaag ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc
    35641 aattgtcatg gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt
    35701 taccgcccct aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca
    35761 catcagtgat tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag
    35821 cttgaagatc acaccttata cacagttggc taatctgact ggacaaccag caatcagctt
    35881 gcctactcat ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg
    35941 aagagaagat ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt
    36001 accaaatcca tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt
    36061 gctctaataa ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag
    36121 gaagataaca gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc
    36181 acactgtttc ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat
    36241 ttggcaaaag aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg
    36301 gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt
    36361 atcacaaaag aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac
    36421 agtatgatcg tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg
    36481 aaaaaagcag ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct
    36541 aaattagtca catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt
    36601 cgaacagatg ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca
    36661 gatatccctg taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta
    36721 ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa
    36781 gaagatggga cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa
    36841 ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta ctttcctcta attgattttt
    36901 ccctcatgtg gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg
    36961 gcttcgtgtc gtttctttta ggaaatctgg tagggttgct tttgacagtt ttaggtcttt
    37021 tggattggct gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc
    37081 ccgcactagt cctgctgttt ctgttatatt ttgggttacc ttatcaatta agtgccttaa
    37141 cggcttcagt catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag
    37201 gatcactagc aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt
    37261 tctttggggt attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac
    37321 tcagtaacgt agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc
    37381 cagaaatatt tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct
    37441 atattttagt tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt
    37501 atttagaaaa acgttctatg catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc
    37561 tggcaccatt tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca
    37621 tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat
    37681 cgaaatagaa tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga
    37741 agtcacgatg cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga
    37801 tggaagccga gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac
    37861 agccaaactg tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga
    37921 agaagtaaaa actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt
    37981 tgaagaggag atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga
    38041 gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt
    38101 aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt ccaacagatc aattgtacaa
    38161 agaattgaaa agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg
    38221 gtttttagag gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct
    38281 tcagcatgat tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc
    38341 attggagtga aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca
    38401 ttaaaagaag cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca
    38461 gtacgtgatt ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcga
    38521 atcgctgtga tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac
    38581 agttggacag tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat
    38641 ctcagtgaag tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca
    38701 aaagatacag aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat
    38761 actagtcgct ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa
    38821 acgagaaagc atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat
    38881 ctttttttcc acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt
    38941 tatttcgata aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct
    39001 tacggcaata tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa
    39061 gcattgaagt ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat
    39121 caactcttga ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc
    39181 cctggactgg gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac
    39241 ctgctagatc atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa
    39301 gtgatccaat gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta
    39361 tattcggcaa acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc
    39421 ggatttcatt gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga
    39481 aacgattttg aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat
    39541 tgctggaaca gctgcattag tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag
    39601 ttggaaagaa tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac
    39661 tcgaggaaaa aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa
    39721 aaatatccaa gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat
    39781 ctattctgct ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag
    39841 aaaagcatat caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat
    39901 taacactact gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc
    39961 ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt
    40021 attttacgaa atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt
    40081 gtcacgacct ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt
    40141 tttttatttt atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg
    40201 ggatagcttt caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg
    40261 aaatctgttc agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga
    40321 tcgtaataaa gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat
    40381 ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac
    40441 tggaaccata tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca
    40501 tctgattgaa tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc
    40561 ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga
    40621 gtgaagtttt tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt
    40681 ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag
    40741 taactttgct tttttgtata ttataccagc aagccagtca caatagcact taaaaagctg
    40801 actaaagtcg ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct
    40861 tcatttaatc ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa
    40921 acgaggaaga cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct
    40981 aattctgtca tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca
    41041 tccactgctt ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct
    41101 aggatctctt ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc
    41161 aaagcaacaa aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga
    41221 atgtattctc ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca
    41281 tcttcttttt cttctaatac atagggattt acaattgaag ccagaatata accgccaaat
    41341 aagttcaaaa caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg
    41401 acgatcgaca tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca
    41461 gataggaagg gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca
    41521 gaagctactc cattataaga ttccaatttg cccatcccgt tgattttact taatcctaga
    41581 ccaacagctt tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca
    41641 gaaataaata caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct
    41701 acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt
    41761 tttgccaata cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga
    41821 acaagctgta aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg
    41881 ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc
    41941 aaataatcac tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta
    42001 atctttagtg aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa
    42061 ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa
    42121 acgaaactat cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta
    42181 ttggtaatat agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt
    42241 tttcaatgtt tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc
    42301 aagttcagct agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga
    42361 taaagcgatc aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga
    42421 tttcaatcct tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat
    42481 atgggctaaa tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact
    42541 tgtacgcccg gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc
    42601 ttttgtagga ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt
    42661 tcggtcttca ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc
    42721 ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc
    42781 ttgtttagag gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac
    42841 acccatgtca gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg
    42901 tttgattaca ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc
    42961 atcaaagtgg aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata
    43021 agcaatgttc agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga
    43081 gtaaccagct gcatgtcctt tttggctttc atgatacagt tggttcaagg aagtagcgag
    43141 ctttccactt cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata
    43201 ctcatttttt ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc
    43261 gatttctgag tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt
    43321 cgtggcaggc tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc
    43381 acgtaattca tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg
    43441 gatcttatta cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt
    43501 caatttttgc agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc
    43561 atgcttgtcg tcaactagct tcccaccaaa ttctaaatat aatttgtcat agtggttcac
    43621 tcgttccaaa atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt
    43681 tttcactagg ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca
    43741 actggaagag cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga
    43801 tagttattca ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga
    43861 aaaagaaaaa cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc
    43921 ttgaagatcc cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa
    43981 ctcgtgatga gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa
    44041 taagataaag gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa
    44101 aaaaaggctg taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt
    44161 ataacaattt tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag
    44221 taagaggagg caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa
    44281 ctagaggaga cgaatgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc
    44341 aaagaaaaag caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa
    44401 gctgtccgcg aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa
    44461 gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc
    44521 catgatcgtt ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg
    44581 atgagctatt acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca
    44641 agtctgccag aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa
    44701 ccggagtcaa cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct
    44761 cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat
    44821 ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta
    44881 gagaagaaga agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg
    44941 cctgaaacaa tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt
    45001 cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag
    45061 ggattcggtc aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat
    45121 aatcaatcta atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa
    45181 aaatgaagaa caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag
    45241 taatcctctg gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacacgaaaa
    45301 agaaaaagta actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac
    45361 tgaaaatccc gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat
    45421 tgaaacagcg gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga
    45481 ttttccacaa ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac
    45541 gatcggaaag acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga
    45601 tattggtctg gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat
    45661 caattatacg ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga
    45721 tcaacgatac caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat
    45781 caatgtccaa gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt
    45841 ctctgcaggg cagatcacga tattcaatca caaattgaat caattattag cccatgcaag
    45901 aacacttggt tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa
    45961 tcttcagagc gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat
    46021 gaaaaatcca tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga
    46081 taaaggacag tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc
    46141 atctggagac tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc
    46201 attaatggtg agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt
    46261 ctgttccaaa ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt
    46321 actaaatgga cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa
    46381 taaaatagca cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca
    46441 atttattccg aatttccagc caatgactgc tattttttta tttatagtac tttacttaag
    46501 cttaaaagat gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg
    46561 tgttggacca tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc
    46621 aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt
    46681 tctggcgggc atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt
    46741 gcctcaacct tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg
    46801 caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg
    46861 aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt
    46921 tgacaagttg cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa
    46981 catcgcaaaa agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag
    47041 tgttcttcaa aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag
    47101 aagataacgg cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct
    47161 attggatgtt tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga
    47221 atgatggaca atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta
    47281 aagggcgaat attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg
    47341 aaaattttaa aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag
    47401 ttgctatcag caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa
    47461 gcatccacag tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag
    47521 ccaataaagg acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca
    47581 gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt
    47641 ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac
    47701 tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag
    47761 aattgaatac tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa
    47821 tactgattat ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag
    47881 gaacaatatt gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga
    47941 tcggtttctt gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca
    48001 tcggtgaaag cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg
    48061 agatcagtcg gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg
    48121 cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata
    48181 ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag
    48241 ataaatatga cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa
    48301 tgagaaaaat ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac
    48361 aatgagtccg ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa
    48421 gtgcgccatg gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag
    48481 taggattagc ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac
    48541 tgttatcgat tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga
    48601 cgcaagcatc atttgcaacg acattattga tggttttctt tttagaatta cgctatacat
    48661 tattaggagc aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct
    48721 tttctcaatc actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc
    48781 cttcctggaa taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg
    48841 gtagcaatat gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc
    48901 attttgcttt gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac
    48961 tgatctttac aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa
    49021 atactttcgg attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga
    49081 aggcattcaa aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc
    49141 cgctcttcaa cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt
    49201 ttagttgctt gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt
    49261 actcatcgaa aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta
    49321 tttgcggcgt tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg
    49381 aatatcaaaa tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg
    49441 gcgttgtcag ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt
    49501 ttattttatt aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc
    49561 atttgcaaca gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg
    49621 gcttatgatg caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat
    49681 tttttgctat aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg
    49741 aataaaaaat tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt
    49801 ttaggggtag tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg
    49861 cttgctacga cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt
    49921 acagtcggat ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat
    49981 aacgctgctt gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc
    50041 gcctctgctg cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct
    50101 aacgttaata atgggatact agctatcaga agtttatgcc catctttgat tgctatccca
    50161 attcttagtg caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact
    50221 tctttgatta tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg
    50281 cgcgtattga ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc
    50341 ttcggtgggt tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc
    50401 gaacttaaag attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct
    50461 gtctctatca ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt
    50521 caaatgatcg accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca
    50581 gatcagcaac tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg
    50641 atcctgatct ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg
    50701 aagaaaaaag atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta
    50761 ttgattcttc ttccttcatt gactggaatg tctttattag ctggaccact atacacattg
    50821 ttttttggct atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg
    50881 tttttctctt tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt
    50941 gcgatccgat taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta
    51001 ggattagttg gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt
    51061 gttcgcggct atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc
    51121 aattttttct taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt
    51181 ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt
    51241 gtggctgtag gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg
    51301 tttcgaatgt tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg
    51361 ttttttgctg tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt
    51421 tccaatttta gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt
    51481 ctttgaaacg aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat
    51541 ctgccggaac gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg
    51601 gatgggaaaa tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta
    51661 aaaaacagta aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta
    51721 tatgaggaat tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg
    51781 atttctggct ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc
    51841 attccttatg actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta
    51901 gacttaaatg atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga
    51961 gataatacct acgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt
    52021 ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt
    52081 tctgaaggga aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga
    52141 gctgaagaaa ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg
    52201 atacatgcaa ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat
    52261 ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa
    52321 aaagccatcg gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa
    52381 aatattccaa catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag
    52441 atggtgtttt tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc
    52501 tataatgtcg catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa
    52561 taaaaggatg aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa
    52621 tcgttgtaga agactatgat aaagttgatg gaagaaatgc attaaatacg gatgcaaaag
    52681 ggctttcact gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg
    52741 tatggcgaca tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta
    52801 ttttagattt agcgatctta gctgcacaag gaagtttata cttccaagat gcttatcgtt
    52861 atgaaaaatt ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc
    52921 gaatgacggt agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt
    52981 acgatacact acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc
    53041 ttttagatga gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga
    53101 gaatttctca ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa
    53161 gaaaccgaaa agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg
    53221 gaattggcaa gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc
    53281 ggggaagaca caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt
    53341 gacctgtttt gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga
    53401 ttctaataag gaacaagacc aagaattact agaagctatt taccaaacag atacacttga
    53461 agctgtactg acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa
    53521 attagcagag ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta
    53581 ttacccttct gaaatcgttc gagcgattga aaaaggatat ttattagaga ttcaagagcc
    53641 gactgtcatc cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg
    53701 gatgttgaat ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac
    53761 tacaaatcga aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca
    53821 acatgcggaa aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa
    53881 aactatgatc caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga
    53941 tgaaactgca aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg
    54001 ggcaaatacc tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta
    54061 taaattatcc actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg
    54121 attgctactg gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga
    54181 gcatacaaaa ggaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa
    54241 tgctgaagaa attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga
    54301 gggagaagag aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa
    54361 agaacgcagt agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc
    54421 tcaagaaatg tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa
    54481 taaagaagcg cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca
    54541 ccgaccagct ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc
    54601 aatagtagaa tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac
    54661 gtaccaaaaa ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga
    54721 tttgagaaat tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt
    54781 acggatcgat gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc
    54841 agcgattgcc attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga
    54901 tacagcagac cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga
    54961 cggatttcaa tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga
    55021 tggtgctgca cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact
    55081 gttgcttaat attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc
    55141 aaaagaagat atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc
    55201 cgcagctatt ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt
    55261 agatattact gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct
    55321 atgataaaat aaagctcagc gaacgcactt agcagattcg ttgagctttt cgtttctagt
    55381 taccggtaac aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag
    55441 gagtgaaacg atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc
    55501 tatcctgcta gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt
    55561 gaaaaactta attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat
    55621 caatctccat ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga
    55681 ttcggaacga tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa
    55741 gtgattatgg atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg
    55801 aaaaaatcta aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc
    55861 aaagaaccga ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa
    55921 actggacagt attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc
    55981 gagaaattga aagaagaact attcaatatg atccgatggt ggttggataa agggatagat
    56041 ggatttcgtt tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca
    56101 gagaaccctt tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg
    56161 aaacatgttt ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca
    56221 gaagaaggac cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat
    56281 catatccata tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat
    56341 gcattaagcg cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa
    56401 aaccatgatg ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct
    56461 atgtctgcaa aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat
    56521 caaggacagg aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca
    56581 gtagattcga aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca
    56641 ttggatatca ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg
    56701 gaacaatatg ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa
    56761 acgatcaatg tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac
    56821 atgatccgca ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt
    56881 catgaacatc cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt
    56941 atggtaaact taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc
    57001 tggacattac tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca
    57061 ccttatgaag ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag
    57121 ttggactttg cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc
    57181 tctaggacca ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag
    57241 ggttttcaaa cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt
    57301 gtcaccgata tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa
    57361 ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac
    57421 aatttcgaaa ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg
    57481 ttcacaacct tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg
    57541 gatgatccat tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc
    57601 gcttgtgctg catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat
    57661 gtcattaact atttcagtca attggtcttt taattctttt tctgacaact tcattttcta
    57721 aaaccttctt tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta
    57781 taggaaaggc aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca
    57841 ctccagccgg ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta
    57901 agcttcctaa taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag
    57961 caacatatcc ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg
    58021 aaatcaaatc gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat
    58081 aaagaaaagc agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg
    58141 gtacagggat caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga
    58201 tcgttaattg cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca
    58261 cttccggtat gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta
    58321 ctttctgcta ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa
    58381 gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc
    58441 tcataatgat caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg
    58501 cctttgatca caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt
    58561 tctgttaacg aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc
    58621 gtaatcatcg taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg
    58681 atcacagctt cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt
    58741 tttgttttta tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt
    58801 ttgataccat ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc
    58861 aaagatatag attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag
    58921 gcgcttagtc cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca
    58981 ccagcaaatg gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg
    59041 aataagagta taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc
    59101 atccaattta aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat
    59161 agttaggact tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta
    59221 gctgttcgcg ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca
    59281 tggacttgct cccaccatcg attccaatcc atttctttat caggagataa agaaataatc
    59341 ggttgtggag aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa
    59401 tctaataaaa agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc
    59461 cgatctttca aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac
    59521 caatcgccat aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt
    59581 cctttagaca gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa
    59641 tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt
    59701 gttttcaaac tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca
    59761 tatacttgaa gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc
    59821 tggttgttta tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga
    59881 tgctccttag cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt
    59941 ggccggtgag cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc
    60001 ataggaaatc ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga
    60061 gagatgtgaa aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat
    60121 ttcgtccgcc atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta
    60181 ccagaagtat gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac
    60241 aaagcaggtt gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa
    60301 atttctctca ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga
    60361 tcgataaata cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca
    60421 ccaaggtaag acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata
    60481 ataactttgt ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca
    60541 ctttcgcagc attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc
    60601 tcatcaaaga taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat
    60661 aagttcaaag gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag
    60721 taatatacaa attcaagata atcgtaacat aatcctttat ataataacat agtttattgt
    60781 gaaaaaaaga tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg
    60841 tgataagaaa aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat
    60901 cgattaccac ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa
    60961 cgatatgttt ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa
    61021 atttggcgaa tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa
    61081 ttttcgtccg tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct
    61141 tttggtattg gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg
    61201 gtcaaagata catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac
    61261 agaagaatgg cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct
    61321 ctgcttgttt gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct
    61381 gcatcgatat atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa
    61441 atctcttctt caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat
    61501 tgcttccttg aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct
    61561 gtagtttgga acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt
    61621 ttttctaaat ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa
    61681 tcagtaaaca gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa
    61741 cgattttctt ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac
    61801 ttttcttgca gttgttttct agttttgctg tccattacaa attccctcca atagtgttca
    61861 ttttctatat tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga
    61921 agcaaatttt taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt
    61981 attttattag tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg
    62041 tggttacttc atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct
    62101 tttatttccg aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa
    62161 taatggtttg tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg
    62221 tacttcatta ctttacatga agatttattc tatggtagga gcaattatat tgagaattaa
    62281 tcctttttta tataagttgt ttgcttattg atggattgga taaattaaac catcacaacg
    62341 agtggaaatt atatgaaatt tttcataaaa attcatatag aaacaaatga cattttatta
    62401 gaatatgact ttcattaaca taaatgtttt caaatttcaa ttaaattgtt ttaatcaaat
    62461 tccaatctaa atatttgtag aaaacaatcg tctcctatag aataaattta taagtaacaa
    62521 tataccaatc ataaatctat ttgctaggag gaatttacat ggaagagaaa aatttaacgg
    62581 tcatcggaag ctatagtacg agggaagaag cattatcagt aattgaacgg ttgcgtaacg
    62641 aagggtatga acgagatgat atcgttattt atactactga tgaagcagca tctagactag
    62701 gatttgacgg attgtctggt atagatgttg aaacagatga aaatcggatg gatggtgaag
    62761 aagaccgttc tttatgggaa aaaataaaag atactttttc ttttgatacc tatgattctg
    62821 agacggctac accagaaaat gatcctttat atcaatatag aggagatatt tctgatggaa
    62881 aatttgtcat cacagtaaaa ggctatcgtc agccagaaac aacagaagat acaatggata
    62941 cacaagatac tgttagccca gcaagcacaa tggggactgg attagaaact ggcactggaa
    63001 cagatccaat ggacgtaggt ggcacgacag gctttcaaaa tacaacggat ccattgaata
    63061 tgacaccagg aactgatccg atggacgtac ctggcgagac tgatacaatg ggtacaagca
    63121 agactgaaga catcgatcgc aaaccaaatc tagatgatga tacaattcaa ctaaaagaag
    63181 aaaaattaga tgtaaataca catgatgtga caactggaga agtcgacatc cacaaacatg
    63241 tggtcaacga tactgaaacg gtagaagtac cggtcaaacg tgaagaaatc gtcatcgaac
    63301 gcaaaccagt aacagatcaa tcttcacagg gaacagacga aaacttggaa gatgacacga
    63361 ttactatccc aatcaaagaa gagcaagtag atgtaagcaa acatacagtt attcgtgaag
    63421 aagttgggat tcataaagaa gaacatgaag atgttgaaaa agtgacagaa gatgttagcc
    63481 gagaagaatt ggatattgac acaagcggtg atgtacacat tgaagaccgc aataaaaaat
    63541 cgtaagcgta agctaaagag gttgtgacag aagtggtcag cttaaagaaa tttcggctta
    63601 tttccgaaga agctacttct ggaacaccgt ttattcgcaa caaaaaagtg ctgtgacaag
    63661 atttttgtca cagccctttt ttagggataa gcattatttt ttaggcttca acggcaattg
    63721 gactttaaaa tcgactcctt gtggaagata attggataca gaaatctgtc catgatgctg
    63781 ttcgattacc tgttttgcaa ttgccaagcc aagcccgtat ccgccagtct ctttcgcccg
    63841 agattgatct tcccgataaa atcgatcaaa aatccttgct tgatcttctt cactgataga
    63901 agggccagta ttctttacgt gaatcagcca ttctttatgg ttaacagatg aatccaaaat
    63961 gatcttatct tctggattcg tatattttaa tgcattatcc agcaaaataa ccaaaacttg
    64021 atggattttt ttttcatcaa ttggtacttc taatgttgca tcgttatgga gaataaactc
    64081 ttttccatct atggtggcca tctcttgaaa tggtttaaca agttgttcga tatattcttg
    64141 tgtattgatc atttgctttg aaagcagctg ctgatcagaa tcattccgag cgattgtcag
    64201 caaatccgaa gtcaatccag tgaggcgcct cgtctctgac aatgcttgag cgatgctttc
    64261 ggattcttca ataatagtat gattaggctt ggtaaaaaga tgttccaaac tattttgaat
    64321 aatcgttaac ggtgtgcgca attcatgaga agcattctca acaaattctt tttgtcttct
    64381 ccaagaaaga ataatagggc gcatgcttag actagacaag taataactga taccaataga
    64441 gagaatccaa aaaacaatca tacacaaaat cataatcgtg cgaaaagtcg ctaatgaatt
    64501 cttgatttga tttgtatttt ctaaaatttg gatgtaggca acgccatctt gattcgtctc
    64561 cgcttttttc gtaatcgaac ggaaagagag ggcagatgag ccactgtctt catttaattc
    64621 aatattttgg atggtattca gatttttcgt atccattttt aattctttga tttggttgaa
    64681 acgcccacca atagctgctt tattcaatat ctcaccattt ttagaccaca agatcacttg
    64741 agtgttaaaa cggtcatttg atggttccat taatggctgt tccatgtttg ttctgttttg
    64801 ttccgtttga gtcgggttta aaaagggatc gccttgctga tatctttgta tctctaattg
    64861 tactgttcga gaatcaaaat ttattttctc taatgacaga tctgtttctc tgtatgcaga
    64921 ttggtttaat atctgtaaag taatgaatcc caaaagaaaa aagataaaag caaatgctgc
    64981 tacatttgtc gcaaaaaagc gactacgttg ctttcgtgta atatctccat tcattgcatc
    65041 acaccctttg cagtatataa ccgacattac gtaaggtttt gatttcttta tccaatccag
    65101 tacttcgcag atgttttcgt aagttgctca tataaacttc aataactgta atcgtcgtat
    65161 cagaatcaaa tccccagatc cggtcaaata tctgctcttt tgtcaaaatg attcccttat
    65221 tttgtaaaaa ataaacgagc aattcaaact cttttccttg tatgggcaaa acttgttctt
    65281 tatacaagac ttcattgctt tttaaatcac aactgatatc cttatacttc agaacatttt
    65341 catcaaagag acctagcgag cgtttcaata atgctttcac gcgcatgatc agttcttctc
    65401 gatagaaagg ttttgttaga taatcatctg cacctcgttc aaatccttca atcttatctt
    65461 ccaaactatc ttttgcagtc aaaatcaata ctggaggctg tattttttct ttccgtattt
    65521 ttgctaacac ttcatatcca ttcatttccg gcatcatcaa atccaagatt gcaagatcat
    65581 aaactccact ttccaactcg aacagtcctt cactaccatc ataaacttgt gttatctcgc
    65641 cgattgcagc gagtatttct tttatattat tggataatac tttatcgtct tctactacta
    65701 atagtttaat cattttccca cctcattgat ttggtcactt tagtttatca tggaaagcat
    65761 aaataaggct taaataaatt ttttatgact catgttaagg ttttatcgaa agatgtctta
    65821 gacaatttat tgataagaaa aaaacaacac aagtgacaaa ttgtcacttt agagttacac
    65881 tattcaacaa gtgacatttt gtcactatca atctatcata gggaggaaaa acaaatgttt
    65941 ctagctatca aagaaattcg ctattctaaa ttacgatatg gcttgatcgt cggtattatg
    66001 ttcttgattg cgtatgtcgt attcatgttg tcaggactag cgaatggact ttcagaagag
    66061 tttaaaaaag caatcgatga ttgggacgca caagagattg ttttgtcgga ggatgcaaat
    66121 caagtatttg cagcttcaca attaacaaga ggagatctga aatacattga aggaggagag
    66181 aaagcaccaa tcggattgta cagcggagca atcaaaggaa aaaacaaaga aaatgttaca
    66241 gttttcggca caacgaaaga agcattttta ttaccaaaac tcacgaaagg gcatgaattt
    66301 aaaacaaaga atgaaatcat catctctcaa aatctggctg attcaggcta taaaattgga
    66361 gatacgatca aaattggcag ttacgacgat cctttgacga ttgtcggtat ctttcctgaa
    66421 acttattaca cagtttctcc cgtcatctat gcgtccctag atacgtggac tgctttgaag
    66481 tatggacaac agccgtttgc ttctgaagaa gaacagccga tcaatgggat cgtaataaag
    66541 gattcagcta aaatttcgaa agaaaaagca tcgaagggtc tacagttatt aactattggg
    66601 acattcattg aaagtattcc tggctattct gcccaaaata tgaccttaaa tgcgatgata
    66661 tactttttgt tcttagtggt tgctgccgtg gtcggtattt tcatgtacgt gatgacactt
    66721 cagaagactg ctatttttgg agtaatgaaa gcacaaggga tcaaaaattt cttcatcgct
    66781 aaatctttag tagcacagtc ctttattgtt ggggtggtag gcgttctgct tgctcttttg
    66841 tttgcttatt tgactagtct gattttacca agcgcgatgc cttttgctgt tttttggtca
    66901 caatggttgc tatatagtgg gattttgatc attgtcgcaa tgctaggcgg attgttttcg
    66961 atacgtacga taaccaaagt agacccaatt acagcaatag gaggttaata aaaatgaaaa
    67021 aaatattaac gatgaaaaaa gttaccaaaa catttggttc tggtcataca aaggtctcag
    67081 ctttgaaagc aattgatttt tatgtaaagg aaggagaatt cgtcagcatc attggtcctt
    67141 ctggttcagg taaaagtact ttccttacca tttcaggagg attgcaaaca ccgacttccg
    67201 gagaaatagt gatcaacggt cactcactaa ctgagcttcc agaaaaaaaa cgagcagatt
    67261 tacgctttaa agaaatagga tttattctcc aaagctctaa tttgattcct tttttaacgg
    67321 taaaggaaca atttgaacta gttgaccaag ttacttcaaa aaaagaagat tcaacaaaaa
    67381 tcgattcctt gctgcaatct ttagatatcc agtcacttaa aaatagttat cctaaagacc
    67441 tttctggtgg agaaagacag agagtggcaa ttgctcgcgc tttatataat gagccaagct
    67501 tgatcttagc agacgaacca acagctagtt tggatacaga tcatgcatat gaagtcgtca
    67561 aattactagt taaagaagct catgagaaac aaaaagcgac tattatggtt actcatgatg
    67621 tccgtatgat tcaatggagt gatcgcgttt atcgaatgga agatgggtac ttgacagaag
    67681 aagagaaaaa gaaggaatga atccttcatg acaatgaagg gctatccacg tataatcaga
    67741 gataaaggag agatgtggat gccaaaagaa acttttttca atttatcgaa cgcaaagaaa
    67801 cagagaatcg atgacctatt actggagata ttttgctcac agcacatcag ccaagtcaaa
    67861 gtctcagaaa tcgttgacaa gatggggatg tcgcgcggtg ctttttataa atattttcaa
    67921 gatttggaag atgcttattt gtatacgatc aaaagcagtt ctcacatagt ccatcaagat
    67981 atcttaaaat tcattagcga aaacaaacaa catttctttt tggggatcga aaaatattta
    68041 gagtggtgct gcgacttaga tcacgaaagt atctattggc aaaggttata cttgttaacc
    68101 cagcgaaatg atcagcagac aaccaaacgt agagaactat cggctgattc tccaatgaac
    68161 gaaaaatggc tggaattatt aagaatcaat catttctcca ttcaatcgtc ggaagaagca
    68221 gttagtttcc tatacttttc catgaccctc gtaatgaaca ctttgacgga tctcattgca
    68281 aataattgga caaaagaaga gttgttaaaa gactttcgct ataaatctcg ttggattgct
    68341 gaaggaatac aataaaaaga aacagctatg tgtatttgtc ctgaaatttt tatcagggtg
    68401 gtttatacat agctgttttt tgattattca taactcgaat aagcggatga gttcttcttc
    68461 atttgcgaat gacacactat catcaatcaa tatatgagga acatgggcat tcaaaatggg
    68521 ttgattcgta acgatcagat catactcgag aagattgtat ttcgacagct gatcttcagt
    68581 aatagcagtt gtctcagagt gaattttatg cacagcaaga atatcactga tatattcttc
    68641 aatatattga ctatgtcgaa gacttaagtc gctgataatc agtatttttt tatcagaagt
    68701 ataatggtga atttcaggat taccgataag catccaaaag atatagctag ataaataata
    68761 aatcagattc tgattcagta gagtggaata aaattgaagt tctgcacgaa atccagcata
    68821 tagacgtatc tggcggtgtt gcatctgctg tgcaaagtaa gttgcacgga tcgtcaaatg
    68881 agtcgtatcg tatgggaaaa tacgggagaa aaaataaatt tgcgttaata attgaacaac
    68941 atattcttcg tttttttctt tgcaatcagc ggatgaaaac gaacagtagg acatattttt
    69001 tcgtatctgc tcactgattt catttttttc tttctccgaa aattctgaaa aaatcgtatt
    69061 gacaaaatag ttcactactt ttatatagct gctgtacttc aattttccta agagattttt
    69121 caagattaag aagtcttttt tatcgatatt tatcaactgt acataagagt ctccagcttt
    69181 ccattcaaat ggagttcctt gggattctct catgagatgc acaacaaaat acataataaa
    69241 gaaagattgc tcatatgggc tttcttcctg aaacaaattc atctgacaat tattcttgac
    69301 cataatttct ttgatcttct ttttttcgat tggaaatggc aacgtgcgtc ttccgtagta
    69361 acttaaaaag aaaaaggtga caaagatacg caactggagc tcacttttaa ggctagttaa
    69421 ctgaaaacct tggtcaacga tcacttttaa taaatagctt tctaaaattt gattgcattg
    69481 tttcaactta cgtgtaaaag aagaccggct gatggaaatt ttttcacaat aatactcaat
    69541 cgttttaccc ggattgacaa taatctcatt taataaacga ctgtttgtag aactttgcga
    69601 catttccatc agaacattta aataagctgg tacattcaaa tgttcaagac gataccccaa
    69661 tttttgtgaa gattgaatcg ttacatccgt tccccagcgg attttgatct cttcgatata
    69721 ccttacaaat gtagattctg aactaccaat tcgtttggca agttcttttt tagtcatcca
    69781 cttgtcagaa atggacagaa ttcgaatgat tttttccatc tttctatagt ttcattccaa
    69841 caacctgatc atatgttttt ctccctcaac ccttttcttt caatgaaacg ctcattaatc
    69901 gaaggaatca acttcataaa tgaaaccaat tggtatgaaa attggtgtga ttccctttag
    69961 cgcccttcgt ataataatta tcatattaaa ataaataaaa ttatgtaacg cctgtttatt
    70021 ttaataattt tcactattca cgtcaaaaca gaaaaaaaga aaaaggaggg gaaaaatgac
    70081 gaccacaggg aagaaactga aagttatttt catgctgata atattgagtt tatcaaactt
    70141 tgtgccatta tctgcaatag cagacactac agatgatcca acagttttag aaacaatttc
    70201 agctgaagtc atttcggatc agtctggaaa aaaagcactg aacatcaagc taaatgcgaa
    70261 taacaccagt gctgaaaaga tagaaaaaga aattggtcta gtcgaaaatt acttaagtga
    70321 tgtggaaaga aaagaaggag atggctatgc ttatcaggta aatagcggga aaattacgtt
    70381 ggaaatctca tcaaacacta aacaaactat cgatctgagt tttccaatcg atccagcact
    70441 ttaccacagc caggcaaaca agctgatcgt cgataataaa gaatatgaca ttattgatga
    70501 gacagaaaat aagaaagata cagatgtgtc agtaccaaag ccagacgaaa tagaagaaga
    70561 atcatcaaaa gaaaacgaaa attctgtcag cccatttaca ttgcctacat tatccttgcc
    70621 agctgtgagt gtgccatcta atcaaacgat tcctacagaa tatacaacag atgatcaggg
    70681 cacttatcct aaagccagtt ggcaacctac aggaaataca aatgttcttg atcatcaagg
    70741 caataaaaac ggaacaaatc aatgggatgg tataaattct tggaatggag atcctaatga
    70801 tcggacccat tcgtatatcg aatatggagg aaccggtaat caagcagact atgcgatacg
    70861 aaagtatgca aaggaaacaa gtacacccgg attgtttgat gtttatttga atgctcgtgg
    70921 aaatgtacaa aaagatatca cgcctcttga tctcgtattg gtcgtagact ggtcaggaag
    70981 tatgaacgac aataatcgga tcggtgaagt aaagattggt gtcgatcgtt ttgtcgatac
    71041 tttagcagat agcggtatca cagacaaaat caatatggga tatgtcggct actcaagcga
    71101 aggatatagc tacagtaacg gtgcagtaca gatgggttca tttgattcag tgaaaaatca
    71161 agtaaaatcc attacacctt cacggacaaa tggtggtact tttacacaaa aagcactaag
    71221 agatgcagga agcatgctat ccgttccaaa tggacataaa aaagtgatcg ttttgctgac
    71281 ggattgtgta ccaacatttt cctataaagt acagcgggta cacgcacaat caagcagcaa
    71341 ttattacgga actcagtttt ctaatacgca agatcggccg ggaaatactt ctctaatctc
    71401 aagaatctat gatgcacctg accaaaacaa tctatccaga agaatcgaca gtacgtttat
    71461 cgcaaccatc ggagaagcga tggcactcaa agaacgagga atcgaaatac atggtcttgg
    71521 catccaactt caaagcgatc cggcagctgg tctctcaaaa gcagaagtag agtctcgtat
    71581 gcgacaaatg gtttcatcag atgaaaaagg cgatctttac tatgaatcag ctgatcatgc
    71641 aacagatatc tctgaatacc tagccaaaaa agctgtacag atctcagcaa ctgtaagcaa
    71701 tggacaaata aatgatccaa tcgcagaacc attcatttat cagcctggta cactttcagt
    71761 caagagtgtg gggacaagtc ctacaacggt cactccatct atttccatag aaggaaatac
    71821 catcaagagc aatcagatct atttaggaaa agaccaagaa atccaaatcc attaccaagt
    71881 gagaatccaa acagaaaatg aggacttcca tccaaatttc tggtatcaaa tgaacggcag
    71941 gacaactttc cagccaaaca ttgataccaa tgaattagct gaattcggta taccatctgc
    72001 taaagctccc ggagtcagtc ttcacatcaa aaagttatgg gaagaatttg acaacaatct
    72061 agctgatcgt ccagatcaag ttacttttga gattcaacgg gaacatacga caaatgctgc
    72121 agcttggaaa aacggatata ttcgaatcat taaaccagct aaagatacta caaattcgtg
    72181 ggaacgtgca gacattgaca aattatctgc aaatagcgga gaaagttatc aagagatatt
    72241 atcactacct caatacaata atcaaggtca agcattcagt taccaaacaa tcaaagaatt
    72301 acctgtacca ggatacgatt ctcaacaaat agatgcaatg acatggaaaa atactaaaca
    72361 attcacaccg ttaaacttga aaataacgaa aaattcctct acaggtgaaa agaatctcat
    72421 cggtgctgtc ttcaaattaa caggagattc tattgatact ttactaacag atcatggcga
    72481 cggaacctat tctcttccag aaaatgtcaa attgcaaaaa gaaatgacct atacgctgac
    72541 agaaacaaaa gctccagaag ggcatggatt aagcaaaaag actacttggg aaatcaagat
    72601 cgcttctgat ggtacggtaa ccattgatgg aaaaacagtc actacttccg atgatacgat
    72661 ccagttgact attgaaaatc cttttgttga agttcctgta gcagtacgta agtatgcgat
    72721 gcaagggacg gacaaagaga taaatcttaa aggagcagca ttttccctac agaaaaaaga
    72781 agcaaatggt acttatcagc caattgacag ccaaacaacg aatgaaaaag gtcttgccag
    72841 ttttgattca ctcacacctg gtaaataccg agtcgttgaa acagctggtc ctgccggata
    72901 tgatacttcg ccgggaaatt atgaattcca aatcgataaa tatggaaaaa tcatttacac
    72961 gggaaaaaat accgagatga caaataatgt atggacgctc actcatcaaa atcgactaaa
    73021 agcgtttgat ctaacggtac acaaaaaaga agacaacgga cagacattaa aaggagcaaa
    73081 attcagactg cagggaccag aaatggactt agaatcgcca aaagatggac aagaaacaga
    73141 tacctttcta ttcgaaaatt taaaacctgg aacttatacg ctgaccgaaa cttttacacc
    73201 agaaggatac caaggtctaa aagagccagt tactatagtt atacacgaag atgggtcaat
    73261 tcaagtggat ggacaagatc atgaatctgt tctgtcacca ggagccaaaa acaaccagat
    73321 ttctttagac atcacgaatc aggcaaaagt accattacct gaaacgggag gaattggccg
    73381 tttaggaatc tatctagtag ggatgattgg ttgtgcgttt tctatttggt atcttttttt
    73441 gaaaaaagaa agagggggca gctaaatatg aaaaaacttg gttggcttag tatgtgtctc
    73501 ttcttgttac tatttaaacc agcttttact caggcagcaa cagaaacaga aacagaaatg
    73561 gttcagatta ctttacacaa attgcttttc ccaaacgggc aactgccgaa aaatcatcca
    73621 aatgacggac aagaaaaagc gttattacaa acgtatcgag gattaaatgg tgtcacattc
    73681 caagtttatg atgtcacaga ttctttttac catctacggg aaaagggcaa aacggtagaa
    73741 gaagcacaaa cagagatcgc aaaaaacggt gcgtcttccg gtacgtttac cgcagaagca
    73801 acaactacaa ctcttaacaa cgaagatggt atcgcttctt tttctctggc cgctaaagat
    73861 caagaaaaaa gagataaagc gtatcttttc attgaatcca aagcaccaga agtcgtcaaa
    73921 gaaaaggcag agaatatggt agttgttctt cctgtacatg gacaaaacaa tcaaaaactt
    73981 tcaactatcc atttgtatcc taaaaatgaa gaaaacgact accctgatcc accttttgag
    74041 aaggtattag aagagcctag aaatgatttt acgattggtg aaaaaatcac ttattccttg
    74101 catacgacaa ttcctgtaaa tatccttgac tatcaaaagt tcgaattgtc agatagtgcg
    74161 gatgaagcat taacgttttt acctaatagt ttaacgattt catcgaatgg agaaaagctg
    74221 acagaaggct ttgtcataca caaaaaacct cacggatttg atgttttatt ttcgatccct
    74281 tcgttggaaa aatatgctgg aaaaaaactg accatttctt atcagatgca gctaagcagt
    74341 acagcacagg cgaacaagga aatcaacaac aacggaacac tggattttgg ttttggtatc
    74401 agtacaaaga aagtctctgt atatacaggg agtaagcaat ttgtcaaaat cgagacaaat
    74461 aaaccagata aacgattagc tggcgcagta ttccttatta aaaacaaagc aggaaattac
    74521 ctccagcaaa cagccaacgg atacaagtgg acaaagaacg aatcagatgc gcttcacctg
    74581 atttccgata aaaatggcgc tttttcaatt tccgggttga aaacaggaag ttatcgatta
    74641 aaagagatcg aagcaccttc tggttatatt ttaagtgaaa cagaaattcc gtttaccatt
    74701 tcaacttttc tttctgagga taaagaggcg gacagtatat tgaaagtagt caataaaaaa
    74761 gaaaatagcc gtccatttct tccaaaaaca aacgaaacga aaaatacact tttaggcgtt
    74821 gttggtatgg tattcgcaag ctttgcaatc tggttgttta tcaaaaaaag aacaggagtg
    74881 aaaaaatgaa aaatcataaa aaaataaacg ttatgttagg agtccttttc cttattttac
    74941 cattactcac aaacagcttc ggcgcaaaaa aagtgtttgc agaggagaca gcagctcaag
    75001 tcatccttca taaaaagaaa atgactgatt tacccgatcc tttaatccaa aacagcggga
    75061 aagaaatgag cgaattcgat caataccaag gattagccga tatttcattt tcagtttata
    75121 acgtcactca agaattttat gcgcaacgag ataaaggagc gtccgtggat gcagcaaaac
    75181 aagcagtcca gtctttgact cctggtacac cagttgcttc aggaacgaca gatgctgatg
    75241 gaaatgtcac tttatcttta cctaaaaaac aaaatgggaa agatgcagtc tacacgatca
    75301 aagaagaacc aaaagacgga gtgtcagctg ccgcaaacat ggttttagct ttccctgtat
    75361 atgagatgat caaacaagca gatggctctt ataaatacgg gacagaagaa ctagatacta
    75421 tccatctcta ccctaaaaat acagtcggta atgatggaac gttgaaagtt acaaaaatcg
    75481 gtactgccga aaacgaagca ctaaatggag cagaatttat tatttctaaa gaagaaggaa
    75541 gaccaagcgt caaaaaatac atccaaagtg tcacagatgg attgtacact tggacaacgg
    75601 atcaaaccaa agccaaacat ttcattactg gtcattctta tgacatcggc aacaatgact
    75661 ttgccgaggc aacgattgaa aaaggtcagt tgatcgtcaa tcatttagag gttggaaaat
    75721 ataatttaga agaagtaaaa gctcctgata atgcggaaat gattgataat caaacaaaca
    75781 cgccgtttga gatcttggca aatagtcaaa ctcctgtaga aaagacaatc aaaaatgata
    75841 cgtccaaagt tgataaaaca acacctcaat tgaatggaaa agatgtcgca atcggagaaa
    75901 aaatccaata tgagatctct gtcaatattc ctttaggtat cgctgataaa gaaggaacac
    75961 aaaacaagta cacgacattc aaactcattg atactcatga cgccgctttg acatttgatc
    76021 atgaaacttc aggaacgtat gcgtatgtct tgtatgatgg ggataaagaa atcgatccaa
    76081 caaattattc tgtcactgaa caaacaaacg gattcacggt ttcagttgac tcgaattata
    76141 ttccttcact cactcctggt ggtacgctga aatttgttta ctatatgcat ttgaacgaaa
    76201 aagcagatcc aaccaaagga ttttctaacc aagcaaatgt cgataacggg catacaaatg
    76261 atcaaacacc accgtcagtc gatgtcgtta ctgggggcaa acgatttgtt aaagtagatg
    76321 gtgacgttac atcagatcaa acacttgctg gagcagaatt cgtcgttcgt gatcaagata
    76381 gtgacacagc gaaatattta tcgatcgacc catccacaaa agccgtcagc tgggtatcgg
    76441 cgaaagaatc agcaacggtt tttacaacca caagtaacgg tttaatcgat gtgacaggtc
    76501 taaaatatgg cacgtactat ctggaagaaa cgaaagcgcc agaaaaatat gttccattaa
    76561 caaaccgtgt agcatttact atcgatgaac aatcttatgt aacagcagga cagttgattt
    76621 ctcctgaaaa aataccaaat aaacacaaag gtacacttcc ttcaacaggc ggtaagggaa
    76681 tctatgtgta tatcggtgca ggagtagtcc ttctactgat tgctggactg tactttgcta
    76741 gacgcaagca cagtcagatt tagtcttttt gcaagaattg gaaaaaggag cagcggatta
    76801 cgcaaatgct cctttttctt ttattatcaa acagtcatca caaggagaac acgaatgaaa
    76861 ccaaagaaaa ggaaacttct agatctgctg atgcttttct tgcttctctc aggaataggg
    76921 atactagctt atccatttgt tagcgacgcg ctgaataatt atctggatca gcagatcatc
    76981 tctcattatc agcaacaagc ggtcaaagaa aatgaagaag tcatggctaa aatccaagaa
    77041 aacatgacaa agaaaaacca gcaattagca aaagaaggtg gaaatccagg tgcagatccc
    77101 tttactaaga aaaaagaacc tgcgaaaaag gatagaacct attttgagaa acatacgatc
    77161 ggtattttga cgatccctga gatcaatgtc catttgccaa tttttgatga aacgaatact
    77221 gtgctgctag aaaaaggtgc ctcattattg gaaggaacgt cttatcctac ggggggagaa
    77281 aatacacatg ccgttatttc cagccatcga ggactgcctc aagcaaagtt gttcacagac
    77341 ttaccgaaac tgactttagg tgattctttc tatatcgaaa tcaatggacg gacactcgct
    77401 tatcaagtcg atcagatcaa gacggtagaa ccgactgata caaaagatct gagaatcgaa
    77461 aaagggcagg atttcgtgac actcctaact tgtacacctt atatggtcaa cagtcatcgc
    77521 ttacttgtca gagggcaccg ggttccttat catgcaaaag aagttcaaaa agaagtacag
    77581 aaagtatccg agaacaaacg atttttcctg tatggattgt tagctgcatg tatactgatt
    77641 ttcctccttc ttatatatct ttttcgtcaa tcgttcaaaa taagaaacag aagttagcta
    77701 ttcctcattt ctataatata tctattcgaa tcgttccgta aacaaacagg ctgaggtttg
    77761 aaaaagaaaa cttcagcctg tttttatgat gatatcaaag agagcctatc aaacactatc
    77821 tttcttatta ctaaatgagg atcttttctt aagttttccg atattcttta agaaggcctt
    77881 tttgcatcaa ttcttgtttg tttttcaagt tgcggatatt ctcttcaaaa ttttgagaat
    77941 atatcactga gaataacgta tttaatacgt aatcgaaggc aatttgagag gagaaagtcg
    78001 ctactttagc aaaatcatat tcctcctgcg atataacaag acacatatca gcctgtttgg
    78061 ccatttctga atgctgattt ccggtaatca gcaaggtagg ggcctctaca tgcttcaaat
    78121 aggtcattat tcgttcataa tgatggatgc gaccgctgta gctaataaaa atggcgcaat
    78181 cctcgctagt caaattggct gcattccaag aagaatcact gtattcttca gccatgatca
    78241 aaaacttatt caacttgacc attttattct ggaatttcct tgccgtgatt tgagaatcac
    78301 cttttgcaaa aagaaagatt cgctgtgcct tgttcaatac tttaccgatt tgatctaaat
    78361 cttcatgttc aatctgcaat tgtgcttttt ttattgactg gatcgataaa tccgccattt
    78421 tctttgcaat ttgctgagtg ctatcttcac ttgaaaaagg aaaattagga tcaatcgttt
    78481 gattgaactg agcaatcaat tgatgaactt cccgccccag ctcgaaacgg aattctttaa
    78541 atccgctgaa tcccattttt ttgcataaac gaatgatagc tgaatgggaa gtatacgtag
    78601 cttttgctaa ttcttcaatc gtcatgtaga ctgcagcttc gatattccta cggatgtaat
    78661 cgacaatccg tttctctgtc gtggtaaaca actcttgttt tgttagtttt tcttcaataa
    78721 gcatgatctg acctccaaat tctatcttac tgataaacac ttggttccgc tactatcttt
    78781 ttgattttta tccttacttt gaacattaat ttcaccaaac cgaatgataa caagtgtttt
    78841 tctgaacttt tttaccagaa tagctattat aaatactttc tgcgcttttc taggatatac
    78901 tgaaaacatc aataaaagga ggcaccactc atgcttacaa tcgcttatct tggcaatgga
    78961 aagagtacaa atcgctatca tttgcctttt tcaagaaaac ttacgaacaa aatcaatatc
    79021 aaaaaaattt tttccccatc tgaaccagtg tggaaaacat tcgaagacat cgaatatact
    79081 gatcagctgt ctgatctttg ggatgatcct gagatccaat tagttgtcat cactacacct
    79141 agtcaatttc attatgaata tggaaaaatg gcactcgaac atggtaagaa tgtactagta
    79201 gagaaacctt ttgcagaaac gtcaaaggaa gcagaagagt tatttgcatt agcaaaagaa
    79261 aaaggtctat ttatccaatg ttaccaaaac agacggttcg attctgactt cttgacagtc
    79321 caaaaagtaa tcgaaagcgg aaaattaggg gatattttag aagtagaaat gcattatgat
    79381 tatttcagac cggaagtacc agaaaatacg catgaatttt ctactgcgaa cagctattta
    79441 tatggacatg cttgtcatac agtagatcaa gttctttctt atttcggcga accagatgat
    79501 atcagctatg atgtacgtca acttttaggg aaaggacgaa tgaatgatta cttcgatctg
    79561 gattttttct atggaaatat gaaaatttcg ataaaatcca gttatttccg catcaaagaa
    79621 cgtccaagtt tcgtcgttta cggaaaaaaa gggatgttcg tgaaacaaac aaaagatcga
    79681 caagaagaac atctgaaaat gttttacatg cctgatcatg aagactttgg tatcgatttg
    79741 cccgaacatt atggtgtatt gacctatata gatgaacaag ggagttatca tgaagaaaaa
    79801 gtaatttccg aggttggtga ttacagccgt gtctacgaag ggatttacga gacattagtt
    79861 aatggcgcag aaaaagtcat caaagatgaa gaaacaatct tacaaatgaa aattttggaa
    79921 gaaggaatca aaagcattca agaatcagaa ggaaaaatat gacatatttg taagaatcgg
    79981 gtttgttttt tgacgcataa actgtcttat tgtacgatga aaaagtatag aactactagg
    80041 aggaaccaac atgacaaatt atgacggtga aaaacacaaa cgattaagag gttcaatggc
    80101 ccctaaaaca gaagaagaag tggctttagc aggaactaat tctggtgaaa cagaagacaa
    80161 agaagaatca ttagctggca caaactctgg tgaaacagaa aataaagaaa cagctttatc
    80221 tggttcaaat tctgtagaag aggaaacaaa gcctaatgag cccaatcact ttgaaaaaga
    80281 tatcgaaaaa gcggttgaag atgtcaaaga aaaatttgaa gaactgacct caaaaaataa
    80341 acatgatcat cattgatcgg acgaaatcaa ttcataaaag caggagacat ttggtcacct
    80401 gctttttttc tgtatccatt cgatagaaat cgttcggtac agtcgtaaaa aaaatccaac
    80461 tgtaagttgt accaaacacc cctcgattta aaagcgctac catactagaa tataagtagg
    80521 aggtgaacag cgctgtgaac tacaaaatta gttatctcct gaaaacacca agagaaatca
    80581 gtctagaaaa agcagcagag atcatcgaag taaatacaag cgaggcgatc aaacaattaa
    80641 ctccttttgc cgaatatgtg gatgataccc gtatccgtat aaagaaattc cgagcaatcg
    80701 actgggcaga aaaatttttg gatattactc gatttgaagc tgtgtatacc gaacaagagc
    80761 gaagagatct tctttatttg atggtttatt cgactgcaac gtctgtttct gtttttcatt
    80821 ttcaagattt tcttcaggtt tctaaaggga cagtcctgtc agatatcaaa aagataagaa
    80881 atgaattaga agtggaagat tgtcagctgt cgtatactag gaaaactggt tttaggctgc
    80941 tagggtcaga agcaatatta agaagagaag caaaaaatcg cactgctcgt ttgatgcagt
    81001 cacttgctgg aagatttggg ctcacgtatt taaccgcaac cctttccatt gattattacg
    81061 ccaaagttag agatttgcta gataaaacga tttcactatc agatcttcga ttcgcaccaa
    81121 atcgaatgga tgaattagct tattatttga gtttgacaac cattcgaatt ggaaaaacat
    81181 ccatggaact ggtaaaagat gctgaactgt tggcttctct tgcagtcact gacattagtc
    81241 gacaagtatt gactggcctt ggctattccg aacatcaaaa ttcggagatt atatttttct
    81301 ctctttgctt agcatcgatc gttgaaggag atgtccagga gcctgcactt gattttttgc
    81361 tggaatgttc ttcagaagtc atccatcgca tggaattcct gatggccgta gaatttgctt
    81421 cgtttcgtga attattgatg aatgtctttt atcacttggt ccctgcctat tttcgtattt
    81481 cctattcttt ttatttacct aatgtgatga tcgatcaaat taagcatcaa tatgcttcca
    81541 tctacgaaat gacaagaaaa gcattacttc cgttagaaag acgaataggg aagagcatcc
    81601 ctgaagaaga gattggtttt ttcactatac tatttggcgg agagatcaga aaagcggacg
    81661 cagaagaaag aaatagaaaa ataagagccg tgatcgtctg tccaagtggc attagctctt
    81721 cattgatctt aaaaagtgaa cttcagcaat tattcccgat gatctttttt acagagacta
    81781 attcagctga ccgcttacac gaggttgacg aaacttctta cgatattatt ttttctactg
    81841 tacctctcac acttactgat aagaaaaagt ctctctatgt tctgcatcca atcatgactg
    81901 acttggaaaa aaatcagctg atcaatcaag tacaaagaga ttggctgata ccaggatttt
    81961 caatgccttc tgcccaagaa ttgttgtcgg cgctactgcc ttatatcgaa ttaaaagaag
    82021 gcgtagacga agaaaagctc tatcaagtat taaatcgtaa aatgaacaaa cttttagaaa
    82081 agaaggagga tcaacgtcct atgctatctg aattattaac agaagaaatg atccaattat
    82141 cggatcaacc aaaaaactgg caagaagcaa tcacatttgc cgcacagccg ctattatcag
    82201 aaaataaaat agaagaaagt tatatcaaag ctatgatcga gcgcgtggag cagtatggtg
    82261 cgtttatcca tattggcgat cacatcgcat tacctcatgc tcgtccagaa gatggcgtaa
    82321 acgaagtagg gatgtctttg ctgaaacttc aacaacctgt ggatctgctt gataatccag
    82381 cccatcccat tagtttgttc atctgtttag ctgctatcga taatgaggca catcttcggg
    82441 ctttggcaaa tttaacaaag ctactttcta ataaacaaaa tcttcaagat ttattacacg
    82501 catcaacaaa agaagaaata ttagcgatca taagaaaagg agacgaatga tatgaaaatt
    82561 tttgcagtat gtcaaagtgg attaggaaca agttttatgg tagagatgaa catcaaacaa
    82621 gcacttgcag atcttggagc agatgtggac gagtttgaag taagtcatac ggatgttgga
    82681 agtgtatcta gcgatatggc tgattatttc ttcattgagc attctttgaa agatacgcta
    82741 acttctatac ctgatgaaaa gctcgtacct ttgcaatcga tcattgacag cgaagaagta
    82801 aaagaaaaag taacgaagat cttgtcaaaa gaataaaaat gattcaaggg aggaactatc
    82861 aatggataaa ttgttgaatt tgtttgtgtc aatcgtcagt cagccctcca tcctcgtttc
    82921 tttgattgca ctgcttggtc tcgttcttca gaagaagaaa ttttcagaag tgatccaagg
    82981 aactatcaaa acatttgtcg gattcctagt actgatcggc ggtgctggtt taatcagtaa
    83041 ttcactaacc ccttttgctt cgatgttcca aacagctctg catactcaag gagtagttcc
    83101 tagcaatgaa gcagtagtgg cgattgcttt gcaacaatac ggtacaccta ctgctttgat
    83161 catgctagta gggatgatcg tgaatatttt acttgctaga ttcacaagat ataaattcat
    83221 ttttcttaca ggtcaagcaa tgctttatgt ttcttgtcta acagcagtca ttttagtaag
    83281 cgcggggttt actgcaaatt tgtggatgat cttattaggc ggggtcttcg aaggaacact
    83341 attaactatt acgcctgctc tttgtcaacc gtttatgaag aacatcactg gaaacaatca
    83401 agttgcgtta gggcataccg gaaatatcgg ttatgcttct agtggatgga tcggaaagtt
    83461 ttttggaaac aaagaccgtt ctacagagga cataaagatt cctaaaagtt tcagcttttt
    83521 gcgagattct actgtatcga tcatgctctt gatgtcgatc gtatatgtcg cacttgcttt
    83581 gttagctggt cctggatttg ttgaaagaga actaagcaat ggagacaacg ctgtaatctt
    83641 tgcactgatc caagctggaa cattcacagc tgggtttgtt atcgtccttc aaggggttcg
    83701 aatggtgttg gcagaaatcg ttccagcatt ccaagggatt gctaaaaaac tcgtacccga
    83761 ttctaaacca gcattagatg ttcctatcgt ttttccatat gcacctaatg ctgtattaat
    83821 tggctttttt gttagcttct ttgtaggaac actatccatg tttgctatga taggcttgca
    83881 aactacagtt atcattcctg gggtagtagg acatttcttc tgcggagctg cttctggtgt
    83941 cttcggcaac tcgactggcg gtagaagagg ggcggtatta ggttctgctt tcaatagctt
    84001 attgattagt tggctgccat tatttatcct tccagtatta ggagatctta aaatcgcttc
    84061 ttctacattt gccgatactg attatctagt accaggactt gtacttggga aactaggaga
    84121 atacggtcaa atagccttga cagttggaat catcgctttc tttttacttg tcgtattatc
    84181 tagctttctt tttgccggaa aaagaacaaa cattgtttct aaagaacaga attaagcaaa
    84241 cttcaaaata aaaattgctt ggacaatatc agaagaagat ttaaagtctt ctgatatgtc
    84301 gcaagcagtt ttttgtttta tgcatttatt tttgaatagc ttgttaagac aagtggctgt
    84361 tttgctagaa gaggataact tttaccattc attttcactg ttccgcctgt tctatcagaa
    84421 gtaatcacga tcttatcttt tgttttttca agcgaaatct ttccatcttt gataggtagc
    84481 gtacaattca gtgtttcaaa tgaatctaga ttaggttgta tcataaattc ttcataacct
    84541 ggctttgttg gatacaaacc gataaaatag cgtcctaaaa gatagattgg gcttgctccc
    84601 catgcatggc ataagctttt tccataagga tctccataca tactgtactt ttcatttcct
    84661 gtttgagtag ggtcgtattc ttcccagaaa gttaccgcat catgagataa catccctccc
    84721 caataatcaa gaatcgtttg gtagacgata ttcgtctgtc cgagtttgca taaagcatct
    84781 tgttcataga atttgaaata tggagtagta atctgtgtga tagtgtcatt caataagaca
    84841 ttctttaaga tcgattgctg ttggtctttg ttagcaaaat cgaataaaac agcgaaaata
    84901 ttcgcatgac gcgtcacatt tcttctccca gattcatagc aatcgataaa agcgccaagt
    84961 tcttcatccc aaaagtagtc aaataggttt tgtttcaata gttgatattt ttcttgatag
    85021 agatgagtat gataattttc gttctctcca tatgtttcag ctaagatatc tccgcaagtg
    85081 atcatggaat gataggcttt cagtaataag acttgttctg cagcaagcgt tccttcttta
    85141 tccatttcag accaatcaat aaatatccaa tcttttgctc tcccataaat aaaccctaat
    85201 tcattcgttt gctccatcaa gtatctcatt aatgattgca tcttagggta gacgatgcgt
    85261 aaaaattcat agtctttcgt catcatatag tggttttcga taccaatcaa ccacaagatc
    85321 gaatagtcaa cgatagtatt catatgctgc ttgatttcat tttgtcctct taatgctaat
    85381 atcgttcgtt tattgatttc ctcgtcaaaa aaaagatatt gattgatcat gaatgattga
    85441 tacgcatctc cagaccaaat ccagcgatct cgtttgatac cgtcgataaa aaacaaatca
    85501 ctacataact tgaaggtttc ttctgatact gaccagatct gattcagtaa agaagaatcg
    85561 cttgaaaaag cagcttgttt cgaaagagat aaaaattcat gaaaaacttg aagttgaacc
    85621 attccagtag aaacctctgg tataaacaaa tagcgaaaag cgcgtttacg agctttcatt
    85681 tttcttgtca ttcctatttg cttatagtag caataatcga tatccatagc ttctacatcg
    85741 gactctccat aacatagagt gatagattcg atatggtcat ccagagatat tagttctaca
    85801 cagccattca ctgctcgccc aaaatcgaat aagattcctc cgccaatctc cgtttcagtg
    85861 actggatgga cgcattcttt ttgataatag atttgatttg gatcttttgc gatatcgaca
    85921 taaagcggac tccagccagc tggatattct tcaataaaat tacttgctgt ccagccaata
    85981 tctgatttta cttcgtctcc ctcgataaac atcgctggta gccctgatgt atgtcctaca
    86041 aagatacgaa tttttgcttt accagctgga caagtcagcc attttccaca tggatgtttt
    86101 tgtccattaa tttctacata cccaactcct tggatcgtca ctttaaacat ggaaggttga
    86161 tcaagaaagt aatatctctt aaacttgacg ttccgatggc aatcatccat gtaccagtac
    86221 gctggccagt caaaacctcg ttcttcccgt tggaaatttt gtagcagtcc atggcggatc
    86281 tcaaagtctc caggatacca aagccatcta tctttatgtt tttcatttgt cattttacac
    86341 gacctccatc aaatttattt tgttgtcaat tgttctttta tttcttcatc tactcttatc
    86401 actgtctttc tattcgtcct gtgtactact agaaggctaa taagcgcgct aatcaacagc
    86461 aagatactgc cattgacgaa aacagcaagt gttttactcc caagagaagc aaaagagaga
    86521 aagagcaaac catatggtgc aatgaaagta cctaaattag cgccaaccaa taagattgaa
    86581 gtattgaaac tctccgcgta cttgggcgct tggatactta cttggttgaa taagtaagga
    86641 atgaataacg aaaaagaaat tcctgttagg atcgcaccaa acccaatcac atacaggttt
    86701 atcacagtgg caatcaatac aaagccgatt cccatcagta gtaaagaaag tgtaagcata
    86761 tagcgatgtg cttttgtctg aagccagcca aaacaaactc cggcagccat acttgccaat
    86821 tgcatgattg ttaagatatt cgctgcatta gtttcgtttc ctagagaata agtcgtcagg
    86881 actgtggcta atttaatcga tacagcatta tataccacga tgaccagcaa gagaaaaata
    86941 ccataactaa tcattttagg attcgttttg attacttgtc ttgttggggc tgtgccatgt
    87001 gattgaagtt cagttgtttc aggtgtagga acaaacaaaa caaacaagac aagaataggt
    87061 aagctgatgg cgtagactaa aaaggtatat tgccaaccga actgtactag ctgtcctgca
    87121 acaaatgtca ttaaagctgc accaaatcct tgaaatgcac tttgaaaacc aatcatagag
    87181 gctcgttcat ctccgtgata aaaggcagca atcatgctga ctgccaaaga attgatcaat
    87241 ccaaagccaa tacctagtcc agctcttgaa gctaagacaa tactgtaact ttgtgagaag
    87301 agggggagga taccataaat cgatgcaatc actaatccga tgatcacagt ttgtttttgt
    87361 ccgattttcc tagcaataaa actactaatc aaaacaaaaa tagtgaccat aaaagaggga
    87421 gcagtcgtta ataattcaac agcagataaa gaacgcgtgg cataagtagc tgacatactg
    87481 ggaattgcag acgtgactgc accagcagaa gtcaaaataa gagagactga cagcagagaa
    87541 aatttcatca tctttttatt cataagcatg ttcctttcat ttttgtaagc gcttatcaaa
    87601 aatatagtat gaagtggtta ttctctcaat agcggaatca caggaagttc atgtcccaac
    87661 catagaaagt ctttatgaaa ccaaaaatag ctttgcatat aatagcagta cagcgtgaac
    87721 taataagcga aagaaaccgc ttacaaaaag gagggagtca attggaacta gctgaattag
    87781 acattttttt tgcgtaacga agaatcaatc gaaactctgc agcgtgaaca aggaatgaat
    87841 atcaatgatt taaacttagc ttaccatctt cagacaccgc caaaaatcca ccgaggggat
    87901 ctatttcaac agggaaatat tcatatcagt aaacaccgtc gatatgcgga agttcccgag
    87961 catacacatg attttgttga aatcaattat atgtatagcg gtacatgttt acaaattatt
    88021 aatggagaaa ggatagagat ggaagaaagt gacgtggtga tcattgataa agaagctact
    88081 caaagaatcg aatacacagg gcaagaggat attttaatca acatcttatt aaaagaagaa
    88141 accttatcta cagagatatt gaatcatttg gcaagcacaa ataatctact ttcttctttt
    88201 ttgataaatg catcaaaaga aaaaggggaa catacacaat tcattcattt tcgcggcgga
    88261 aaaacaagtc gtttgacgca tttgattgaa aatatgctgt tgatttattt tgatacatac
    88321 agtcataaga tccgttcgtt aaatctctat acatcactat tactgatcga attgactcat
    88381 ttattagaac aacaaagctt ttcatcagca gatggatgcg cagaaaatga gatgcttaga
    88441 gtattagatt atatcgataa gcattttact tcttgtacgt taaaagaaac agcagctcat
    88501 tttggctaca atccggtcta tcttagtaat aaattgaaaa tgtattttga tgcttccttt
    88561 caagaactgg ttctaaagaa aaaattcgac tttgcgttgg aactgattca agaaacgagt
    88621 ttttcgttgg aagaaatcgc taatcgtatc ggatataaac aaacgacttc tttattcaaa
    88681 ttattcaaga agtattcaac atgtactccc aatgaatgga gaaataaaca ttaagaagaa
    88741 aatctttcac taaagcatat gaaggaaaat tgttttaggt gcgattgctg tcttattata
    88801 tcgttgcagc gaattttttt ggatttattt tcagttctaa acttttggtt gtattatcag
    88861 gggatatagg tgtaaacatt tgtcatttat ctgttatgcg tattcaggaa ttcgtttcca
    88921 tcaaaaatta taataacaac tattataatt aacatgttga taaaatgaat aaacaagact
    88981 atacttttca tgaaagtaac aaatagttat gaaaaggagc tgtttattca tgaaaaaaac
    89041 aagtcgtaaa gtagtaatcg tcggtacagg ttttgttgga acaagtatcg cttatgcaat
    89101 gatcaaccaa ggaatctcta atgaattagt attgatcgat gtgaatcaag aaaaagcaga
    89161 aggggaagca ttggaccttt tagatggtat ggcttggggc gatgaaaatg tggctgtatg
    89221 gtcaggtggc tatgaagaat gtaaagatgc tgatatcgtt gttatcacag ctggtatcaa
    89281 ccaaaaacct ggtcaatctc gtttagattt agttaaaaca aatgcgtcaa tcatgcgcca
    89341 aatcgtaaaa gaaatcatgg gatcaggatt cgacggtatt atcgttgttg cttcaaaccc
    89401 agtagatatt ttgacatata tcgcttggaa cgaatctggt ctgccaacat cacgtgttat
    89461 cggtactgga acaacattgg atacaactcg tttccgtaaa gaaatcgcat tgaaattaaa
    89521 agttgaccca cgcagcgtcc acggctatat cttaggtgaa catggtgatt cagaagttgc
    89581 tgcatggtca catacaactg tcggtggtaa accagtgttt gaaattgtag aaaaagacca
    89641 tcgtattgcc caagatgaat tagatgtgat tgctgataaa gtccgtaatg ctgcttatga
    89701 gatcattgat cgtaaaaaag caacttacta tggtattggt atgagtactg cacgtatcgt
    89761 aaaagctatc ttgaataacg aacaagctgt attgcctgta tctgcttatt taactggtga
    89821 atacgacgaa aaagatatct ttacaggtgt gccatcaatc gttgacgaaa atggtgttcg
    89881 tgaagttgtt gaattatcaa tcaacgaaga agaaaaagca atgttcagca aatcaacaag
    89941 tgctttaaga gaagtattga acactgtatt ataagaaaca atgaatatga actaaaaatc
    90001 ctaaaactgc ttaacagctg aaatagctgt taagcagttt tttattgata tctaataggt
    90061 aagcaaataa acagaatggt taaaattaat cggtccgaaa atgtatccgt acatatgaac
    90121 atatgaaatt taatctgttg attacaataa gcgaatcgtt atcaatgttt atctgtggtt
    90181 aattaaaaaa tccttccgca acggcaaata caatgattca tatctaattc gttcatattt
    90241 gattaactga ataaagaaac tggtttttat cattcaatta aacgacaaaa aaagtaagaa
    90301 aaactgcaaa atttttttat aattttgcat aagcagattt tgatggaaaa atgacgattt
    90361 ttgataaaaa accgttcgag agaggtttat cattttttaa atctactttg tataatatat
    90421 attatgtaaa ctaaaatata taaaaaatca gcccactagt tctaaaccac tatttcctac
    90481 tattcactct tttgtattcc ttttaaatca attatttcaa aattaatcca tattttccat
    90541 atttttatga aaaaactatt ttgtatgatt aaacataaaa aaatcagcat attccttaat
    90601 gacatgctga tctaattgag aaataaagtt tttaattcgt tcattttatt gcggtttgtc
    90661 gtaaataact ggttatcttg gactttagtg aatcgaaatc caatgaacta gtggtaatca
    90721 ttatgataaa atcaacagct tcttgacgag aaaaagcaac tggacaatta ttcaattgaa
    90781 gaaacaacaa catggcaaca agagcagttc gtttattgcc attatgaaaa ggatgttttt
    90841 tagctaaatt aattgctaaa atcgccgctt tttcatagat ttcaggatag agttcttgat
    90901 tgaatactgc ttgtgaaggc tgtttgacgg ccatatctaa tgcatttgca tcttttacac
    90961 cgatcatttc gcctggcgag tgacgtaaaa taactttcgc gttgattttg ataatttctt
    91021 tagtcgaaag atacctcata aatcaaccaa atcttttaac gcgtcttcat attgattcat
    91081 gatcgaatca actaatgaat caatatcagg ttcttcttgt gctttgatca ttcgtacaga
    91141 accatcagat tgaaaaacat aactgattgc gtcacctgtc tgcacaccca tgaattcttt
    91201 tacttctggt gggatagttg ttacatcgct attgccagat tttctaattt tatacttttt
    91261 catttttttc atatttcgcc accctctata ccattctcat tctatagtaa gtacattata
    91321 tccaagtata tacttttaat caatgtatat acttttattt tgctttttct tacgaaaaaa
    91381 tgacaaagaa aaaagtttgt gacatgactt ttgtcacaaa ctctcgtatt cttaaccaaa
    91441 atattgatga aatacttgtg taataactgg tagaaaataa ataatgatca tagccgctac
    91501 caccaccaac aattgttttc gctgtttctt tcgctttagc atgcttgtcg ttttatgaaa
    91561 aaaataagta gcgcttttag ctcctgcagt caaagtcgat aaaatcaacg ttggtgaata
    91621 tgctgtttga ttgtgcgtca atgacaacaa cttcgtcccg taatcaggat actttagaaa
    91681 tgcttggtac acagccacaa tcgtgattgc tcccataaga taatttaaaa atgaatgcac
    91741 ttttttcaaa taaaaaaact ccttgctatc tatataaaat cagcctaccc caacgttttt
    91801 gcttgattca ctgatatttt ttagttttat tggtatttat attctcactt ttcggaataa
    91861 atattccgct atttcattgt tttcatttac tctacttcgt tttcattatt ttttcttgat
    91921 gtttctttgt ttccatcaac tgcttttgtt ccactgtttt cgatccatgg aaaagaaaaa
    91981 ccgattgtca tatcttcttt taatactaaa actgcatcga atttcccttt tttaccagta
    92041 aaacctttga caactttact tgtctttcct tttgaaagca aatctctgag attcgtttta
    92101 gtcagcttct ttttagctac tgttggccac aatttgaagt cgcattccgg attcaagcat
    92161 gtagcgattt taggataaag catgactatt ccctttttac attttggaca tcttcctaat
    92221 tgatcgtttt tcttctccat cgcttttatc tgacgtgtcc cgctaatctc atctgaaaag
    92281 tctaccttgt cgatttgatt aggaacttct gccaataaat gtaggatgaa ttttttgata
    92341 ttctctaaaa aaacagatgg tgtcccctct tgtttaccaa tttttctcaa gtacccttcc
    92401 cagcgcgcag tcatttctgc gctcgtcagt aatggttcat tggctactgc cttacaaaga
    92461 gtgattcctt ttggcgtaac agttatttcg tttttattga cttcgatgta atgcttctgc
    92521 ttaagatttt caatgatatt ggctcgtgta gcttctgtcc ctattccttc tacttctttg
    92581 agaatttctt gcgcttcatc attatccagc gtttttcccg cagttttcat tgctgtgatc
    92641 aacgttcctt cggtataggg ttttggaggt tgtgtttctt tctccatact tttcagttct
    92701 acttctgctt cttcattttc tacgatagga ggaagagtct gagcttcttt cttttttgac
    92761 ttgatgcgta agatttcctg ccaaccttgc tgtaatggga tctttccgat gctttttagc
    92821 tttaattgct gtacgcccgt cattacggtc gtctcttcgt agatataatc tgcagcaaac
    92881 atagccacag tagtgcgcaa aacttgcaaa tagattgctt gctgtaattt ggaaagcttc
    92941 gcaaagcgtg caggagtcgg aatcgtcttt gtcggaataa ttgcatgatg ttcttgaact
    93001 tttttcgcat cgacatatcg tttctttgct ttgatctcag ttgtctgtac ttcttctatt
    93061 cctaaaaatt ttttataatt ttgcagatgt ttaaccagat actgatgctc attttctgta
    93121 atgtaaaagg aatccgttct tggatagctt agaaacttgc tttcgtacaa tccttgagca
    93181 gcatcaagtg tagctttggc acttgctttc attagctgat tcatctttgt ctgcaaacta
    93241 gatagcgaaa ataaacgtgg actatgcaac ctcttttcct ttttcgtaac ttcaagaatc
    93301 cgtccgtttt gcttacctaa atgcgctcca agtttttcta tttccttcgt taactcttct
    93361 tgggttttaa atgctttttt tggtacgatt tttccagaaa actgcccttt gtttgtcgta
    93421 attaaacctt ctccttcaaa gtacgtttct tttttgaagt gtttgatttc ttcttgcagc
    93481 tgataaatca tatagagtgt cggtgtttgt acacgaccaa gagaaaaact gccatcaatc
    93541 ccttttcgtt gaagtagcaa gctatataat ggacttccat tcattccaat cagccaatca
    93601 gcgttctgcc ttgcttgcgc ttctttatat tttggaaaat agttctttcc aggttggaga
    93661 ttcttaaaac cttcatagat tgcttctttt tcaagagaat tgatccataa acgtaaatag
    93721 ttcttttcct tagaaaatgc gtttgcttgt cgaataatcg accacgcgat ggcttcacct
    93781 tctcgatcac catctgttgc aataatgatg gtatcggctg actgcaattc tcgttttacg
    93841 atcgaaaatt gtgcacgttt gtccattgga acttcgtaac ggaaggtttc tgggaaaata
    93901 ggcaaatttt ctaaagacca ttttgcccaa cgctcttcat aagctcctgg agcaaccata
    93961 tctaccaaat gtccaaaacc ataagtgatg gttacttcac cagcaaacaa tggatcgctg
    94021 atttcataat acccttcatg ccgcttttgc tgttggaagg tttcggcata ggcttttgct
    94081 tgtgaaggtt tttcagctag tatcaccgtc ttcatcttct gacctccgtt acttaatttt
    94141 ttgcggactt ctctattatg gaacatattt tcgcatctgg caataaaaaa aagataccgt
    94201 atagtcgaaa tagacacaaa aacatcaagc aaacaaacga tgatccgttt gctgcttgat
    94261 gtttccagaa aatattcatc tttagctgtc actcttggtt aatctgccag ttgatgccaa
    94321 atcgatcagt taccatccca tacaactgag cccacggaac agcatccagt ggcatctcaa
    94381 tcttaccacc ctcagctaag ctttcaaata ggcgcttcgt ttcttcttca tttcttgtct
    94441 ggatgactaa agaaacatta tttcccacaa ctaattcagg accaattcct tctggcgtat
    94501 ctgcaaacat aactaatgta tcataaatgt tcagccgtgc attcgtgatt agattttttg
    94561 cttcttctgg caagttcatt tgttctattc caggcatttc accgtatgtg gtgatacctg
    94621 atgcttcagt ctgaaaaact ttttcataaa aagcgatggc ttcacgcgac tcatttttga
    94681 agttcagata gatatccaat gtaaacagct cctctcttgg ttaaatctac cataagaaag
    94741 cgttttttac gagtgaatcg tttgattgat tcatgacaaa agccttgcgc agcttcttct
    94801 tgatcttttt tatgatcatt tattcaaaaa atctgtaata gcagattgat tcatttgatt
    94861 atcaatttca agaatactgc cagcataatc actttgccca taattccacg aaccgtctac
    94921 tggcacactt attcgatcaa tgccaccagc tcctcgtgca atcgaaaaga ggtttttcgt
    94981 caagaaccaa gcagtgacat ctgtggatgt atatcctaaa gctttaccag ctgcataagg
    95041 tgcacggatc agattcaatg gattcttcaa ttggctgaaa atagcgttca ttacttgctg
    95101 ttggcgtctg acacggccaa agtctccttg ttcatccatt cggaaacgtg cgtactgcaa
    95161 aagaacatgt ccatccatct tctggacccc ttttttaata tctacaccat ccagattcag
    95221 atctttttct gcatcgatct tcactccgtt catgaataag gcatcgatca ctttttcaaa
    95281 cgatttgaaa tcgactttcg catagtattg acaatcgatt ccaaaatttt ctactaaagt
    95341 ttgacgcact agatcggctc ctccgtatgc ataagctgaa ttgatcttat tttgtcctac
    95401 ccctgggatc gtaacgaagc tgtcacgcat aaaagaaatc agtttcggct tatgggaagg
    95461 accatccaac tgtaagacca tgatcgtatc ggctcgcccg gcatcttctc cgcgtgtatc
    95521 acttccaagg atcaaaatat tatgcgcacc attcgtcgct ttttgcccat gaaaggtttc
    95581 tgaagtcgct gcctctaagc ttgtgtcatg ttcagctaca tatttaccaa taaaaaatga
    95641 gatacctgaa taaaggatca aaacaagcaa aatcgataga atgatttttt tccacgatcg
    95701 ctttcttcga ggttttaacg gtttgttctt cttttgccga ttgaaaaatt ttttattttt
    95761 ttgttgttct tctgctcttg gtgcatctct gtatatctgt tgctcgtatt ggttcaaatg
    95821 ttcaaatgaa tgctgctctc tttcataaat acgggtagca tcttcctcat tttccatacg
    95881 cgttcgtttt cgttctcttc tagcaaaaat actccccgta tcccgcttca gaggtttcgc
    95941 cttcttatgg atgtccttat agcgatccat ccgactcatt gtggttcctc ctcgtttctt
    96001 actgataaat aaaggatacg ataaccaatc gacaaaagcc tatcattttt tgataattta
    96061 ataaaaaata acgaaataaa tgcttcgata cataaaggat tacttttctt gagaccgttt
    96121 atgcgagaat aaaagatctt tatctattat ccttttttaa tcaaacagct tttcaatcac
    96181 atggatcggc atcttttcct ttgtccataa ttcaaacgcg attgctcctt ggtagatcaa
    96241 catgccaagt ccattgtatc cttttgctcc tacttttttt gcttctttta ataaacgtgt
    96301 ttctctaggt gcataaatca aatcaaaaac cgctagattc tcatgcatca tgcttatatc
    96361 taagagcggt gactgattga ttttctttcc catcccgata tctgttgcat tgactagaag
    96421 acagctttct ttcaagtctt ttcttaattg atgcgtatcg gcaaagtcac gaacttttat
    96481 cttacaagaa gttcgatagc tgagttgttc gaatgtttct tttacttgag cgaatgtttg
    96541 gttgtttcgt ttataaacta cgatttcctc agcgccgtct aacgcagctt gaacagtgac
    96601 tgcctttgct gcacctccag ctcccagtag agtcattttt ttctttttga tcgaaatccc
    96661 agcttcttgc acggattgca tgaagcccgc accatcggta ttatagccaa tcaaacgacc
    96721 cttttcgtgc acgatcgtat tcactgctcc gatcaattgc gcttcctggc ttacctcatc
    96781 cagataagga ataaccgctg ttttattcgg catcgaaaga tttacaccta acatatcaaa
    96841 agttcgaata ctttcgactg cttgcttcaa attcattgga tttacttgga aagctaaata
    96901 caccgcatcg atgttgtttg cttcaaaagc agtattgtgc atcaatgggg ataaactgtg
    96961 agaagccgga tcagcaaaga gcccagctaa tttggtttgt ccagaaatca tcttattcct
    97021 cctttgttct tacttcaata aaaaaagcca cttagcgttt gatacgaaaa agtacgaaac
    97081 gtctaggtgg caaagtagca aattcttttc tcatcgttta cttgtcagag caaagaatat
    97141 tctatttaat tgttaaaatc attgactacg tgatcctaca aaacacccaa atatgtttaa
    97201 ttgtttattg ttttaagtat aggatgaatc aaaggaagat gtcaatgagt tttcaagtcc
    97261 ttctactgaa aggaattttt ctgtcgaaat caatccacta cttcttcaaa gaactgcaaa
    97321 aggtcgccca ttgtcaaatg ttgcttttct ttttcagaaa tatcttgaac gattttccct
    97381 ttttggagta cgatcagacg atttccgtat ttcaaggcgt cttccattcg atgcgtgatc
    97441 atgagacaag tcaatttttg ttcattgatc cgctgatttg ttaatcgcat taatgcttta
    97501 gctgttttag gatctaaagc tgcagtatgt tcatctaata atagtaactc aggagctgtg
    97561 atcgtcgcca tcagcaagct taatgcttgg cgttgaccac cagataattc acccgctgga
    97621 gtatccaagt gttgctctag gccattaccg acttcttgac atagtctgaa aaaatcttct
    97681 ttttgatttt tcaagttacg taaacggaat ttccgttttt gacctctttt ttgtgcaagt
    97741 agcaaatttt ctgctactgt catccgagga gccgttccca tcttggggtc ttggaaaact
    97801 cgagaaagga atcgtgctct tttttcttct gaatcttttg tgatcagctg atctttaaaa
    97861 gagatcgatc cttgactgag ttgaagcgta ccagcgatcg tattgaacag tgtgcttttt
    97921 ccagcaccat tcccgccaag gacagtgatg aagtcccctt ctctgattgt aaggttaatt
    97981 tcatccaata gtactttctc ttcattcatt ccattgttta cgacttttct gccatttttg
    98041 attgtcaata tatcagtcat tactatgcct ccttctcaaa acccgttttt aagttcagtg
    98101 ctttcttcaa ttgcgggatc atcagacaaa tagctaagat gatagcggag aacaacttca
    98161 gataagtcgt gtcaaaccct agtttgatta ctactaaaat caatagctga taaatgatac
    98221 tgccgacaac aatagcaatc aatctttctg ctagcgtcaa ttcttggaag atcacttcac
    98281 cgatgatgat ggaagcaaga ccgatgacga ttacgccaat ccctttattt acatctgcat
    98341 atccgtcgtt ctgggcaatc agcgcaccgg ataatgcaat gacaccgttc gacaaagtta
    98401 aacctaaaat tttcatacga tcggttttta tccctaacga ccgagccatc gtttcgttgt
    98461 ctcctgtcgc aatatagcct tgacccaaat ctgtattgaa gaagaatagc agtacagcaa
    98521 tcaagatgcc caaagagatg aagccaagaa ggactgtgtc aaagtaatca ggtaacccaa
    98581 agcgatggaa gctgtcttgt agaatcggat gattcaataa agaaagattt ggtgttcgca
    98641 tcacaaacaa aataactgag ttcaatcctg acatcactaa aatccctgct aaaataaccg
    98701 gtatctttcc ttttgtataa agtaaccccg taacgagtcc tgctatcatg ccagcaccta
    98761 cgcctaataa tgtagctaca atcggcgcta ccccatgagt aatagctgtc acacagacag
    98821 cagcacctaa tgggaaggac ccttctgtcg tcatatcagg aaagtctaag atccgataag
    98881 tcatgaaaat ccctaatcct aatattgccc aaagcatgcc ttgtgaaata gctgatacga
    98941 tcatttgtca tcctccttag atgtttcttg ctgcgagtca tctgtaatga ttttggcttt
    99001 ttccttcatc gattggggaa caatgattcc taagcgatca gcttgagatt gattgatcac
    99061 tgtatcccct tgatcaaatg tataaacagg cgtcgtttcc ggtttttctt ttccactcgc
    99121 aagttctgcc gccatcttac cgctttgaag ccctaattga tattgattga tcccgattgt
    99181 tgctagtcca ccttgttcca ccattgtatc cacagatgga atgacgggga tatttgcttt
    99241 gtttgcttct tttaccactg tttgcatcgc attggcaatg gtattatcta atggaacata
    99301 gataaaatcg acttgtcggc tcatcacttg aaccgtttgt gcaatctcat tagtggaagg
    99361 caccgcatat tttactgatt ctaacccttc tttttttgca gcttcttctg cttgtgctac
    99421 ttggtatttt gaattatcct ctgaagaagc atacaagatt ccgacttttt ttgcatttgg
    99481 caacaaatca gaagcaagct tgatttgtga agtaacaggc ggctgatcag aaacacctgt
    99541 tacattacca ccaggtttct ctaaagaagc gactaatcca gcccccacag gatccgttac
    99601 agcacctaac acaactggga tcgatgaagt cgtgtttgct aatgattgag cggcaggtgt
    99661 tgcgattcca atcaaggcat ctggatcaga ttgtactagc tgttgactca ttgtggctag
    99721 cttgctctga tcggcttgtc cattttgaaa tttgatctca agattttctc cttcttttaa
    99781 tccttcttct ttcagaccat ctttgattcc tcgataaatc tcatctaatg ccggatggct
    99841 cacgtattgc aataccccaa caactgttgt tttttttgtc tggttcgtcg aagtactttc
    99901 tttttcgcct gaggcttttc cataaataaa tgcacctgct aaataaacta aaataattac
    99961 tacaactgtt attaatcgtt tgtttttcat ttttttcctc ctgcaaagtc aatcttttta
   100021 tttattataa tcaggcaagt atccagtcta caaaaaaaga caatggccaa agcggtcatt
   100081 gtctttacac aaaaaaccgc atagattcta atctatgcgg cagtcaatgt tcatgtctag
   100141 catagataca agctttagaa atgcactcta aaaagttgta tctatgggaa agtctcacca
   100201 ttaatacaac ttatctacgc cagctgcgcc aatttttttt gttttctgct gaaatacgtt
   100261 ttgataagtt cattacaggt caccctttcc ttaagaattg atttaagtct aatcgaattt
   100321 ttgaatttcg tcaactatat tttttaattt tttatttgtt attttgaaga tcaaacatca
   100381 cttgttctga taattaaaaa tagcgctata tagacccacc gtttcatcgc gccaatgtct
   100441 caattagcaa tctcggaatt ttgggaagtt actcccgttc tatccaattc ttcttctaat
   100501 tgtttgatat tggctaacgg aagtcgattc agttcaggga tcaactcctt atcaaaatca
   100561 gttattcctc ccttacccgc agttatccga tttgcttctt ttcctccatc aaagaaaata
   100621 aatgtcactt catccgttcg tatgttatat ttttgttcca gttcatgtac atcataaacg
   100681 atcggataat aaaagatcgg tcgattcaat tcttccataa gctcaggatc attaaaaaca
   100741 tcaagcatgt cctgataggt tttcccgttc ggtttgctga acatcaccga tatcgcccgg
   100801 ctttgtttga ttttctgatc agctttgctg taatcaaaag gtttcaattt gctggttggt
   100861 atggacgaat aaacgagact atttacttgc tcttctcgga ttttttccgg caataaaaac
   100921 attgcaactg ttaacagcaa tgataagatg agcagaaaaa caagacgtcg aaaatactta
   100981 cttggatttt ctgcgaatga cactgagaaa tgttcattca tttcttgtac tctcgattta
   101041 actacttccc aaataacaaa caccttcttt tctacttttt ctcctatttt ttatt
//
LOCUS       DACDLI010000003        72654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDLI010000003 DACDLI010000000
VERSION     DACDLI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.003"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..410
                     /locus_tag="IV353_000196"
     CDS             <1..410
                     /locus_tag="IV353_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9008477.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(494..1216)
                     /locus_tag="IV353_000197"
     CDS             complement(494..1216)
                     /locus_tag="IV353_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9008478.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1206..1943)
                     /locus_tag="IV353_000198"
     CDS             complement(1206..1943)
                     /locus_tag="IV353_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008479.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1955..2947)
                     /locus_tag="IV353_000199"
     CDS             complement(1955..2947)
                     /locus_tag="IV353_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9008480.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2962..3705)
                     /locus_tag="IV353_000200"
     CDS             complement(2962..3705)
                     /locus_tag="IV353_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9008481.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3779..3883)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3946..5268)
                     /locus_tag="IV353_000201"
     CDS             complement(3946..5268)
                     /locus_tag="IV353_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9008482.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5255..6220)
                     /locus_tag="IV353_000202"
     CDS             complement(5255..6220)
                     /locus_tag="IV353_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008483.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6217..7755)
                     /locus_tag="IV353_000203"
     CDS             complement(6217..7755)
                     /locus_tag="IV353_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9008484.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8171..8458)
                     /locus_tag="IV353_000204"
     CDS             complement(8171..8458)
                     /locus_tag="IV353_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008485.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8592..9260
                     /locus_tag="IV353_000205"
     CDS             8592..9260
                     /locus_tag="IV353_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9008486.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9551..10291)
                     /locus_tag="IV353_000206"
     CDS             complement(9551..10291)
                     /locus_tag="IV353_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008487.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10421..11368)
                     /locus_tag="IV353_000207"
     CDS             complement(10421..11368)
                     /locus_tag="IV353_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9008488.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11497..12261)
                     /locus_tag="IV353_000208"
     CDS             complement(11497..12261)
                     /locus_tag="IV353_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9008489.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12265..12654)
                     /locus_tag="IV353_000209"
     CDS             complement(12265..12654)
                     /locus_tag="IV353_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008490.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12789..13331
                     /locus_tag="IV353_000210"
     CDS             12789..13331
                     /locus_tag="IV353_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9008491.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13479..14111
                     /locus_tag="IV353_000211"
     CDS             13479..14111
                     /locus_tag="IV353_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008492.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14245..14370)
                     /locus_tag="IV353_000212"
     CDS             complement(14245..14370)
                     /locus_tag="IV353_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9008493.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14504..15397)
                     /locus_tag="IV353_000213"
     CDS             complement(14504..15397)
                     /locus_tag="IV353_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9008494.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15579..16505)
                     /locus_tag="IV353_000214"
     CDS             complement(15579..16505)
                     /locus_tag="IV353_000214"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9008495.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16590..17351)
                     /gene="pflA"
                     /locus_tag="IV353_000215"
     CDS             complement(16590..17351)
                     /gene="pflA"
                     /locus_tag="IV353_000215"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9008496.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17595..19826)
                     /gene="pflB"
                     /locus_tag="IV353_000216"
     CDS             complement(17595..19826)
                     /gene="pflB"
                     /locus_tag="IV353_000216"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9008497.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20103..22553)
                     /gene="parC"
                     /locus_tag="IV353_000217"
     CDS             complement(20103..22553)
                     /gene="parC"
                     /locus_tag="IV353_000217"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9008498.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22565..24610)
                     /gene="parE"
                     /locus_tag="IV353_000218"
     CDS             complement(22565..24610)
                     /gene="parE"
                     /locus_tag="IV353_000218"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9008499.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24776..25210)
                     /locus_tag="IV353_000219"
     CDS             complement(24776..25210)
                     /locus_tag="IV353_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9008500.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25337..25975
                     /gene="plsY"
                     /locus_tag="IV353_000220"
     CDS             25337..25975
                     /gene="plsY"
                     /locus_tag="IV353_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9008501.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26107..26982)
                     /locus_tag="IV353_000221"
     CDS             complement(26107..26982)
                     /locus_tag="IV353_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9008502.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27064..27855)
                     /gene="codY"
                     /locus_tag="IV353_000222"
     CDS             complement(27064..27855)
                     /gene="codY"
                     /locus_tag="IV353_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9008503.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27873..29273)
                     /gene="hslU"
                     /locus_tag="IV353_000223"
     CDS             complement(27873..29273)
                     /gene="hslU"
                     /locus_tag="IV353_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9008504.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29287..29835)
                     /gene="hslV"
                     /locus_tag="IV353_000224"
     CDS             complement(29287..29835)
                     /gene="hslV"
                     /locus_tag="IV353_000224"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9008505.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29872..30777)
                     /gene="xerC"
                     /locus_tag="IV353_000225"
     CDS             complement(29872..30777)
                     /gene="xerC"
                     /locus_tag="IV353_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9008506.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30975..32351)
                     /gene="trmFO"
                     /locus_tag="IV353_000226"
     CDS             complement(30975..32351)
                     /gene="trmFO"
                     /locus_tag="IV353_000226"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9008507.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32320..34398)
                     /gene="topA"
                     /locus_tag="IV353_000227"
     CDS             complement(32320..34398)
                     /gene="topA"
                     /locus_tag="IV353_000227"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9008508.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34520..35377)
                     /gene="dprA"
                     /locus_tag="IV353_000228"
     CDS             complement(34520..35377)
                     /gene="dprA"
                     /locus_tag="IV353_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9008509.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35433..36200)
                     /locus_tag="IV353_000229"
     CDS             complement(35433..36200)
                     /locus_tag="IV353_000229"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9008510.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36193..37053)
                     /gene="ylqF"
                     /locus_tag="IV353_000230"
     CDS             complement(36193..37053)
                     /gene="ylqF"
                     /locus_tag="IV353_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9008511.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37313..37711
                     /locus_tag="IV353_000231"
     CDS             37313..37711
                     /locus_tag="IV353_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9008512.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37948..38490
                     /locus_tag="IV353_000232"
     CDS             37948..38490
                     /locus_tag="IV353_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9008513.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38573..39124)
                     /gene="lepB"
                     /locus_tag="IV353_000233"
     CDS             complement(38573..39124)
                     /gene="lepB"
                     /locus_tag="IV353_000233"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9008514.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39276..39503)
                     /locus_tag="IV353_000234"
     CDS             complement(39276..39503)
                     /locus_tag="IV353_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9008515.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39500..40018)
                     /gene="msrA"
                     /locus_tag="IV353_000235"
     CDS             complement(39500..40018)
                     /gene="msrA"
                     /locus_tag="IV353_000235"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9008516.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40038..40751)
                     /locus_tag="IV353_000236"
     CDS             complement(40038..40751)
                     /locus_tag="IV353_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9008517.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40754..41650)
                     /locus_tag="IV353_000237"
     CDS             complement(40754..41650)
                     /locus_tag="IV353_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9008518.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41808..42650)
                     /locus_tag="IV353_000238"
     CDS             complement(41808..42650)
                     /locus_tag="IV353_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9008519.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42782..43435
                     /locus_tag="IV353_000239"
     CDS             42782..43435
                     /locus_tag="IV353_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9008520.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43498..44025)
                     /locus_tag="IV353_000240"
     CDS             complement(43498..44025)
                     /locus_tag="IV353_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9008521.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44045..44992)
                     /locus_tag="IV353_000241"
     CDS             complement(44045..44992)
                     /locus_tag="IV353_000241"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9008522.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45004..46890)
                     /locus_tag="IV353_000242"
     CDS             complement(45004..46890)
                     /locus_tag="IV353_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9008523.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47050..49128)
                     /locus_tag="IV353_000243"
     CDS             complement(47050..49128)
                     /locus_tag="IV353_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9008524.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49141..49416)
                     /locus_tag="IV353_000244"
     CDS             complement(49141..49416)
                     /locus_tag="IV353_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008525.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49778..50233)
                     /locus_tag="IV353_000245"
     CDS             complement(49778..50233)
                     /locus_tag="IV353_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008526.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50387..51595)
                     /locus_tag="IV353_000246"
     CDS             complement(50387..51595)
                     /locus_tag="IV353_000246"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9008527.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51707..52039)
                     /locus_tag="IV353_000247"
     CDS             complement(51707..52039)
                     /locus_tag="IV353_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9008528.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52187..53062
                     /locus_tag="IV353_000248"
     CDS             52187..53062
                     /locus_tag="IV353_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9008529.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53144..53725)
                     /locus_tag="IV353_000249"
     CDS             complement(53144..53725)
                     /locus_tag="IV353_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9008530.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53731..54990)
                     /locus_tag="IV353_000250"
     CDS             complement(53731..54990)
                     /locus_tag="IV353_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9008531.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(54992..56038)
                     /locus_tag="IV353_000251"
     CDS             complement(54992..56038)
                     /locus_tag="IV353_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008532.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56273..56548)
                     /locus_tag="IV353_000252"
     CDS             complement(56273..56548)
                     /locus_tag="IV353_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9008533.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56791..58101)
                     /locus_tag="IV353_000253"
     CDS             complement(56791..58101)
                     /locus_tag="IV353_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9008534.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58283..59512)
                     /gene="rpsA"
                     /locus_tag="IV353_000254"
     CDS             complement(58283..59512)
                     /gene="rpsA"
                     /locus_tag="IV353_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9008535.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59647..60327)
                     /locus_tag="IV353_000255"
     CDS             complement(59647..60327)
                     /locus_tag="IV353_000255"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9008536.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60376..61011)
                     /locus_tag="IV353_000256"
     CDS             complement(60376..61011)
                     /locus_tag="IV353_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9008537.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61077..62480)
                     /locus_tag="IV353_000257"
     CDS             complement(61077..62480)
                     /locus_tag="IV353_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9008538.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(62467..63498)
                     /locus_tag="IV353_000258"
     CDS             complement(62467..63498)
                     /locus_tag="IV353_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008539.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63529..63750
                     /locus_tag="IV353_000259"
     CDS             63529..63750
                     /locus_tag="IV353_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9008540.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63915..64652)
                     /locus_tag="IV353_000260"
     CDS             complement(63915..64652)
                     /locus_tag="IV353_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9008541.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64805..66145)
                     /locus_tag="IV353_000261"
     CDS             complement(64805..66145)
                     /locus_tag="IV353_000261"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9008542.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66382..67905)
                     /locus_tag="IV353_000262"
     CDS             complement(66382..67905)
                     /locus_tag="IV353_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9008543.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68283..68894)
                     /locus_tag="IV353_000263"
     CDS             complement(68283..68894)
                     /locus_tag="IV353_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9008544.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69017..69142)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69224..69940)
                     /locus_tag="IV353_000264"
     CDS             complement(69224..69940)
                     /locus_tag="IV353_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9008545.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69946..70515)
                     /gene="scpB"
                     /locus_tag="IV353_000265"
     CDS             complement(69946..70515)
                     /gene="scpB"
                     /locus_tag="IV353_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9008546.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70499..71296)
                     /locus_tag="IV353_000266"
     CDS             complement(70499..71296)
                     /locus_tag="IV353_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9008547.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71266..71637)
                     /locus_tag="IV353_000267"
     CDS             complement(71266..71637)
                     /locus_tag="IV353_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9008548.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71656..72543)
                     /gene="xerD"
                     /locus_tag="IV353_000268"
     CDS             complement(71656..72543)
                     /gene="xerD"
                     /locus_tag="IV353_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9008549.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT        20065 a        15321 c        12399 g        24869 t
ORIGIN      
        1 aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct caagcagccg
       61 ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt caaacggaaa
      121 aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa actgctgcta
      181 ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca agcggttctt
      241 acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct tacttaaatg
      301 gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact tcacgttatg
      361 gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa gattcattta
      421 ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac actagttgtg
      481 tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga taagtatttt
      541 ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac tgttcttcaa
      601 gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct gctgcatgta
      661 gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt tgccagactg
      721 ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca atatagtctt
      781 gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa gcttggatat
      841 aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc tcatttatgg
      901 ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt acattcgtcc
      961 aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa ggctcaactt
     1021 gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag gcttgagtct
     1081 tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa ttgatgatcg
     1141 tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct aaatgacaat
     1201 gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg cttcaactct
     1261 gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat ctcagcaatc
     1321 acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat tgcttcttcg
     1381 atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt caacatccag
     1441 tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc aagaagcaag
     1501 taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat cccgccagat
     1561 aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag ataatccctt
     1621 acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac gttttgatag
     1681 acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc tgtatggaga
     1741 ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg gactcctgtt
     1801 aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga tgtaaaggta
     1861 tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat gactacattc
     1921 aagtggctga tttggattga catatctatc actcctattt tataaactga ttagtgaata
     1981 gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa gctgtataat
     2041 tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta taattttttg
     2101 acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca tatttttgca
     2161 acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt ttcgtggcat
     2221 ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc gtaataatta
     2281 ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag tttagttcaa
     2341 tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg atatctgagg
     2401 attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt tttgaaaagt
     2461 ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga ctttgccaac
     2521 ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct gatagggtgg
     2581 cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt atattctgtc
     2641 catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt gctttgtccg
     2701 cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg acatttaaac
     2761 cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga ttagtattcg
     2821 gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt ttgctacttg
     2881 tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa agccataatt
     2941 ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc cttttttcaa
     3001 taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga tcgcagcaaa
     3061 aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta agcctgcttg
     3121 agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag ccacttttaa
     3181 accggaaaag aagccggata aacccgctgg tattttatat attcgataaa tcttccctgt
     3241 atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc tcaatgtatc
     3301 cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca cttttggaag
     3361 cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg gcaacatttg
     3421 tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat ccattgccaa
     3481 ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt catttaatgt
     3541 cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa atacagcagc
     3601 aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct gccaaataat
     3661 taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt tacctccttt
     3721 cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac ccgcttcaag
     3781 cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg tctaagttca
     3841 caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt tttaaagtgt
     3901 agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt tcaataaata
     3961 ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact cttttttagt
     4021 caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt gctcgctaat
     4081 cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct tagcaactgg
     4141 ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca cttgatctga
     4201 cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg cgtagacgat
     4261 cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc gcttagctac
     4321 ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg aaacaatatc
     4381 tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg caattccgtg
     4441 tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg gctgtctccc
     4501 agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta aacgatagcg
     4561 agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa tgccagatgc
     4621 ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta atggccgttg
     4681 attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag ctagctcaaa
     4741 agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca atgataaaaa
     4801 gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag gtctttgccc
     4861 ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa tagatgaatc
     4921 atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta attgtttttt
     4981 tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt cttcccagtt
     5041 ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat tacgtgcctc
     5101 tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag tcccgcccca
     5161 tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat aagcagcact
     5221 taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt ccttcctcct
     5281 ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt ctttgctttt
     5341 ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc ataggcgtat
     5401 caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat ccattgacga
     5461 ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa tgccgaacgt
     5521 agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata atttctccat
     5581 ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg tgcgtaacga
     5641 aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt aatgagtttc
     5701 ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct gggcttgcag
     5761 caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct ctaggcatac
     5821 gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct acacggtctg
     5881 ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt tgcttgactg
     5941 tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac cgcattttct
     6001 gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct gatggtttag
     6061 ataatccatt gatcattttc aaagtagttg tttttccact accggaagga ccaaccaaaa
     6121 caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac tttccatcat
     6181 actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct cctttactaa
     6241 tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt cgacattgac
     6301 ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt tattcagtgc
     6361 tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg cgccttgata
     6421 agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct tgagctggct
     6481 atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt ggtaacgcaa
     6541 acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt ggattccttt
     6601 atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct gatcagagat
     6661 agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc tcttgactgc
     6721 caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga attgcttatt
     6781 caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac ttttcattgc
     6841 ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat agatatcgat
     6901 ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg gttttaattc
     6961 aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca ggatttcagg
     7021 ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg cagactgata
     7081 taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg aatatcgagg
     7141 tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca aggcagaact
     7201 gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca aaataaatgt
     7261 ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga ttaaaactaa
     7321 ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta ctttcagcaa
     7381 gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg ggtcaatttc
     7441 agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa caagtgcaat
     7501 caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata aggcaagtga
     7561 aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt ttttatgatt
     7621 ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag aaatgagaga
     7681 aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac ttaatgtttg
     7741 tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac atttctttcc
     7801 tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa aagaaaaaaa
     7861 tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc aactttttgg
     7921 ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac ttggaatcag
     7981 ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc aataaagtag
     8041 aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata ctgtatcatg
     8101 acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg tcttgttttc
     8161 ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa agaggaatga
     8221 gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa aaaagaaaga
     8281 tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat tgatttgctt
     8341 tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa aaaactcctt
     8401 gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct gctcgcattt
     8461 ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt ttcattttcc
     8521 tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt tcaaaagaaa
     8581 agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc attttaggcg
     8641 caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg gcagatggtg
     8701 gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct tctgtcactt
     8761 cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga aaacacgctt
     8821 ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta tgggagagcc
     8881 ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca ggtattcttt
     8941 ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga acagatatca
     9001 tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt ttcttgatta
     9061 cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt ctgtatacat
     9121 tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg gaaattggat
     9181 ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca cttgatcctc
     9241 ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta cagaaatatc
     9301 attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa ataaactatt
     9361 tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt tcttacttat
     9421 tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa actttcggat
     9481 agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg aatataaatt
     9541 tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga tcatcgatca
     9601 atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt tcttctgact
     9661 gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc tcaccatttc
     9721 ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca tccgtttttt
     9781 tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct gggataatcg
     9841 atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg tcaacagatg
     9901 cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca gaacgagagg
     9961 aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa tggccggtat
    10021 aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct gacttcaatc
    10081 catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg aaatgaaatt
    10141 ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct tgtccgtcaa
    10201 ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg tacgcaggaa
    10261 aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa aatggaactt
    10321 ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta ttttaagaaa
    10381 atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt gtctctctga
    10441 taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct tctatttttt
    10501 ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg atcgttgtca
    10561 atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca ccaaattctt
    10621 cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct agccgatctg
    10681 aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc atatatcttt
    10741 ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca acttccttca
    10801 tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact tcgataccga
    10861 tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt gctgtaccat
    10921 attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac aagacaactg
    10981 gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg atgatgtatc
    11041 catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa taatgcgctt
    11101 taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca gttgtgtcga
    11161 tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt gcttttgcag
    11221 caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct ttcaattcgt
    11281 cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa acattggcac
    11341 gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt tgtcattctt
    11401 actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat aaaaaacgga
    11461 aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt ttatcaaatt
    11521 ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat gtcgctgtgt
    11581 ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt tttctacaaa
    11641 tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa agaacagagg
    11701 acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc atgaaatcaa
    11761 atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta cgatttcttt
    11821 tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta tatgaggatg
    11881 tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa ctgcaagctg
    11941 atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt caaacagatt
    12001 tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct gtttgcgctc
    12061 cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa tttttcttcc
    12121 ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga caacgggaat
    12181 acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat ggacagaacg
    12241 tttcaatata attggattca attgttactc acctattttc attaaatttc ctgatcgcca
    12301 tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata atataaatgc
    12361 aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc ctaatgctag
    12421 cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga aaatattacc
    12481 aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca gcgacatctg
    12541 aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta agtatatact
    12601 gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt tcattttaat
    12661 cacctaacct atctttataa ttctattata atcattttat catttttttg tatgtataaa
    12721 gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa atgaaaggag
    12781 ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat tttgcttata
    12841 tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg attgctccac
    12901 ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt ttagatcgtt
    12961 tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt atcagtgtcg
    13021 gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg attttgccag
    13081 caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt tatggtgtga
    13141 cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata ctttctccta
    13201 gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt gcttttatca
    13261 tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg agaaaacgac
    13321 aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt ttaaatgtta
    13381 gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc aatttgttat
    13441 tatactttat atacttatca atttaatttg gagatattat ggaaattcaa cgtagaacca
    13501 ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt tttctatgtt
    13561 attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg cttttggatc
    13621 attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct tctctttgcg
    13681 gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct ggtacgttaa
    13741 acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca attctttttt
    13801 attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc gtgattttat
    13861 ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt ccgctcttct
    13921 ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt gtccttgctt
    13981 taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg gatatgctct
    14041 tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat aaaaagcatt
    14101 ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac ttcaatctaa
    14161 aagaataacc caagattgga taaattatta atgactcgcc attcgtttct tagtgattct
    14221 ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa tgccatactt
    14281 gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta cttcctttgt
    14341 cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg tagtgaatct
    14401 aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag gaagagaaag
    14461 gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt ttactcacta
    14521 aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc ttgctcagct
    14581 cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa ggatccaatg
    14641 ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat gtgaatgatt
    14701 cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta ggccggcgat
    14761 ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca aaaaagtacg
    14821 attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt acaggaagct
    14881 gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct atagaggaaa
    14941 gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct gtctctcggc
    15001 tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa tacgcccaaa
    15061 taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat tgttcagcta
    15121 tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat attggaaagt
    15181 tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat aaatcaagaa
    15241 aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc gtttcttgtt
    15301 ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg gtcagttttt
    15361 gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt tctatccgtt
    15421 ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca taattgactg
    15481 ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta tatcaatcgc
    15541 ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac agccgtcaat
    15601 tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg gttgttggtc
    15661 aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac aagtaattct
    15721 gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc atttgctttc
    15781 atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc attaaggtcc
    15841 actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact ttttgcatcc
    15901 aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt caacatttca
    15961 agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc agctgcttca
    16021 tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat agctgagagc
    16081 atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag ttttaaaata
    16141 atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc tgtttcaaag
    16201 tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat atctgaaata
    16261 ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc tttagctgac
    16321 tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt ttcttcatta
    16381 ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa gtgtgaaaac
    16441 gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac taaaattttt
    16501 gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa aacggaaaga
    16561 agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt atactcttct
    16621 gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc gatcccttct
    16681 aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg ataaggaaga
    16741 atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg gatcaaatat
    16801 tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg tttgttgatt
    16861 tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt tagtttttta
    16921 tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata cttcatcaat
    16981 tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca tgtatccaat
    17041 gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa atcgatctgc
    17101 aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta aggtctgaag
    17161 cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc aatattccaa
    17221 gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa cacgacaaag
    17281 cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata gccgattgtt
    17341 ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca agttagctgt
    17401 cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg tgtaaaattt
    17461 acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat aagcatactg
    17521 attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt cgaaaaactt
    17581 ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc taattgttgt
    17641 tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt caagttcgga
    17701 tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac attgacattc
    17761 aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt ttcgattctt
    17821 gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga aattccatcc
    17881 aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa cccactttgt
    17941 tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc acggccatca
    18001 ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa taatgaagtt
    18061 gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa gtattccaag
    18121 atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg gaagtcgcca
    18181 tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac ttttgcatat
    18241 ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt agcaaatgtg
    18301 cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata tttatcatgc
    18361 atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc catcatattc
    18421 atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg acgatattct
    18481 ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata taatagtgtt
    18541 ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac tgttcctgat
    18601 acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg attatcattt
    18661 tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct gaatccttct
    18721 ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc catatttgtt
    18781 aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc taatcccata
    18841 cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt atattcaggt
    18901 gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa ttcttgtgct
    18961 tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc aaggaatgct
    19021 gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc taaatatccg
    19081 aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat atcatagcca
    19141 tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt gatttcttca
    19201 cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg atcttttttc
    19261 ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc accgatgata
    19321 cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg agcagcacgc
    19381 atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca gtcagtgaaa
    19441 atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt gttagccata
    19501 ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg tagaccgacg
    19561 attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt tgatacgatt
    19621 tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa cttttgcaat
    19681 tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc atcaccgcga
    19741 tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt ccatttggta
    19801 cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt tttatgtaac
    19861 agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt cttgtttttt
    19921 atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca taccaacatt
    19981 taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat taaaaatgaa
    20041 tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa atactacaac
    20101 agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc tccacctttt
    20161 ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa atcagttggg
    20221 tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga tcttccttcc
    20281 gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt cactccccgc
    20341 ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc tcctcgctga
    20401 gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt gataatccta
    20461 tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac aacgggaaca
    20521 tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac atctaagcct
    20581 ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt tagcttcata
    20641 cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat tcgtgtttgc
    20701 ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat tttttgatat
    20761 gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg agaaatgtgc
    20821 tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta gatcacattt
    20881 gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga gtatagcaag
    20941 tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga acgaaggctt
    21001 gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg tgccactaag
    21061 acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag acgagcggaa
    21121 gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag ctctttgtca
    21181 tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga ttccttttga
    21241 agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat tgcttctgct
    21301 tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt tttgtcttta
    21361 ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat caatccttct
    21421 acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt tgttatgact
    21481 tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg atgcggagtc
    21541 atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt gtttttgaac
    21601 aaataattca aaattccttg agcattggcg tcttttttca attcgacgac aatttgcaga
    21661 cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt attcaaacgg
    21721 atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat ttctgtgatc
    21781 acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg aagaataact
    21841 tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa gatccctcct
    21901 gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc ttgtggatga
    21961 tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg gatttctgtt
    22021 gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta tcgagctgga
    22081 aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac tgtttctttc
    22141 tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt ataacgcatc
    22201 gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat cagtacttcc
    22261 cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat gtcaccatga
    22321 ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa tcctttgtca
    22381 tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg cttcaatcca
    22441 tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg tccgaagcgg
    22501 tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc catcaggtga
    22561 cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt tcttcatcta
    22621 ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct aagatactgc
    22681 catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga ggttctactt
    22741 tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg atacggacgc
    22801 ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc gcattcatct
    22861 ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta atcattgctg
    22921 ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt cctttggata
    22981 ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc ggtttcatat
    23041 aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta tccgcatcgg
    23101 tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga ccgactcctg
    23161 caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct tgcattttag
    23221 ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac tttctgtctc
    23281 ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat tcatttttct
    23341 tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg ccttttttac
    23401 gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc gcttctctgg
    23461 ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc aaataaaagc
    23521 ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta ccaagttttt
    23581 cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa agtacagcag
    23641 ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc agcaaaccaa
    23701 cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca gcttcatggg
    23761 ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct gaataaccgt
    23821 cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa aaataaacaa
    23881 ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg attccttctt
    23941 cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga gagattttaa
    24001 cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca tatgaaaatt
    24061 tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg tttgcttttt
    24121 ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta aatttctgct
    24181 gatactctac gccatctcta acgatcgtta caattagcca tttcgataag gcatttacca
    24241 cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga ccaaactttc
    24301 cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa gcatgcattc
    24361 ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca tggatggtca
    24421 catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg acgatttcat
    24481 agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct ggtctttttc
    24541 taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg ttgtttactt
    24601 tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca ccacgattat
    24661 catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt taattatctc
    24721 ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt catttttatt
    24781 tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt acgatatctg
    24841 tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct aattgtgccc
    24901 agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa aattcactcc
    24961 gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc gcatagactt
    25021 tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt ttatatccat
    25081 tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg ctcaagccta
    25141 cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt gttggatttt
    25201 ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat acttgtatct
    25261 ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata gttttcaact
    25321 tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc ttttaggttc
    25381 tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc gacaacacgg
    25441 gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag ccgggattgt
    25501 tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga tgatcttcca
    25561 cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc atacatttcc
    25621 gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa tgcttttagg
    25681 atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt tattttgtac
    25741 aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat ctacgatcgt
    25801 ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga cgatcattgc
    25861 ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc gtatcagaaa
    25921 ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat cctgatactt
    25981 tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc ccttattact
    26041 tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact tctcggtcac
    26101 aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg cacctagttg
    26161 attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag cgattccgca
    26221 ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga tccctacata
    26281 aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc gaatggcgta
    26341 cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt cacgattcaa
    26401 agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga atgggccttt
    26461 caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat cttcaaatga
    26521 caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa agaacatttc
    26581 tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc ctaattcata
    26641 gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg tactccttaa
    26701 gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat cccgagcaaa
    26761 tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat cttttaaacg
    26821 tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat caccttgcca
    26881 gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac cgtgttcagc
    26941 gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc ctccaacttc
    27001 gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc agtcccctta
    27061 cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata cttttaaata
    27121 agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca attttcgtaa
    27181 agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag aagcagtcag
    27241 acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca attcgctata
    27301 agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg cttcgatgct
    27361 tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt ctgctaagac
    27421 tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac ccaaacgttc
    27481 gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct ctcttgactc
    27541 taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt tcatcaactg
    27601 tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg ttttgattcg
    27661 ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac ttcctatgat
    27721 ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt aaggcaaatc
    27781 tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc gggttttcgt
    27841 tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact taaatcttca
    27901 tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt tatttctccc
    27961 atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa aatggtatgc
    28021 agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat ttgagcgatc
    28081 cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat caacgcgacg
    28141 tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc ttcagctgtc
    28201 aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat tagatcactt
    28261 ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt ttggatgatc
    28321 ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct ttggacgcct
    28381 tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat ttcatcgata
    28441 aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc agcatccttg
    28501 actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac tgttaccgtc
    28561 cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc gatccccatt
    28621 tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac ttcaatggtt
    28681 acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg gcgattgatt
    28741 cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact agcattaaaa
    28801 agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt gataccaggt
    28861 accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta tacacggctg
    28921 tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac cattgattct
    28981 acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt gacaaatggt
    29041 gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc tgtaggtccg
    29101 atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa ctgcaaacgg
    29161 cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg tcctacgata
    29221 tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata catatctact
    29281 cacctcttac agttcttcta cgataatatt atgattagta aagatacaga tatctgcggc
    29341 aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag catagttttt
    29401 cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta agataccatc
    29461 atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat cattcattac
    29521 gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt gagctaattc
    29581 gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa atttttcttc
    29641 taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc cattgtagat
    29701 acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg ttacttggcc
    29761 atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg ttgaatgaaa
    29821 tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt tttacgctct
    29881 tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg catagatctg
    29941 ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat ctgccccatt
    30001 atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat cactatccaa
    30061 actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg tgattggttt
    30121 tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca tcaatacctg
    30181 gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta gaggagcata
    30241 tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat caatgtctga
    30301 taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga cttccagtaa
    30361 agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa gtgcttgcat
    30421 ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat gaacgtaaga
    30481 aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg aacgtaggct
    30541 ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag ctaatgcact
    30601 caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc cagattcctc
    30661 caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt tttctgaata
    30721 tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt ctgtcatttt
    30781 ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa gacaaataaa
    30841 ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcacaatt cctcacattt
    30901 tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg tctcaccctc
    30961 ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt ttttattgcc
    31021 tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt gtctcgaatg
    31081 cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa atgttttcct
    31141 tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg aaactcgact
    31201 ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc gctcgctgca
    31261 ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc ttcgcgtttc
    31321 gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt tcggtgcatc
    31381 acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa aacacgtttt
    31441 tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga agctgcagca
    31501 ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg atcttccaat
    31561 ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc cattacttca
    31621 attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc tgccacttcg
    31681 gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg acagttcaag
    31741 taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat atcgatcgta
    31801 gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga accattgaat
    31861 gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac tacggtgatt
    31921 ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg ctctttgact
    31981 tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc accagctggc
    32041 actgctgttt catctgccga agtgattact accgaattca atcggcgcat ttcttctttc
    32101 aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca tactagttca
    32161 gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc atataaacgg
    32221 acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag ccctgctcca
    32281 acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga tgtcttcttc
    32341 gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct cgaccagata
    32401 tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga attcacattc
    32461 tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt caatgacttg
    32521 tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt tcgtattccg
    32581 acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt ttatcaccat
    32641 cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt gaattttttc
    32701 gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac gatcaaccac
    32761 tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca tttctgctgt
    32821 gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga caatttctcc
    32881 tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc tttggatcgt
    32941 atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta gtgcacgaat
    33001 caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg gttcaatatc
    33061 aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt tctcttcttt
    33121 gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga tttttgaacc
    33181 gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat ctaaaatagc
    33241 tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt tcaattgatc
    33301 tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg gacgcacagc
    33361 ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat gtctagaaaa
    33421 ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg tatcggcaat
    33481 ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt tacccaaagt
    33541 gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa agtttaactt
    33601 acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt ttcttttgcg
    33661 ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag cagtgacttc
    33721 ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc agaaatttgc
    33781 tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt cttctggaac
    33841 aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag attggacacg
    33901 tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg aataaccaac
    33961 gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat cgatcgttct
    34021 tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga ccacccgatt
    34081 tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg cttctccttc
    34141 tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg ctttcaggct
    34201 tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat tgttttcaaa
    34261 atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg ccattacttt
    34321 atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt ctacaatcac
    34381 taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc ctactattaa
    34441 aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt gtcaagaaaa
    34501 gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca cccgaaaccg
    34561 tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct gttaaaatat
    34621 ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct gttatcagag
    34681 agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct gcgataatcc
    34741 gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat tcactcaaaa
    34801 gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga tagacttgct
    34861 ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct gcttggtgac
    34921 cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct tctatcactg
    34981 gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt ccaccaacaa
    35041 cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt acaattggcg
    35101 gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg ataaacgact
    35161 gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg cgaaacagct
    35221 cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt tctttataag
    35281 gatactgcat catccagtta gcaagtgccc attttccttg actactgatt ccttttgtat
    35341 aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct ttcgtctctc
    35401 tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt tacaggtgca
    35461 aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa gtgttccttt
    35521 gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc atattctttc
    35581 atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga cacagatcgt
    35641 gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat cgcatcgatc
    35701 aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc taatttactt
    35761 gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat cccgattgcc
    35821 agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga taattgtttg
    35881 gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc tgccacgaca
    35941 ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg gtgaccttgt
    36001 gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat taatgcttcc
    36061 tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg tggatcattt
    36121 cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg ttctttgata
    36181 ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat ccagtgtgta
    36241 tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac gatcatagtc
    36301 ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca atttttcttc
    36361 ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc gggcaaaaaa
    36421 gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga tcgcgcctgt
    36481 cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa tccctggtgt
    36541 gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg ttacgccagg
    36601 cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg tagatttccc
    36661 aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc cccgagcagc
    36721 atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac ctttaattcc
    36781 tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa aatattcttg
    36841 ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa caaggcgagg
    36901 tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta atcgtgcgtc
    36961 taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc gtcgggcttt
    37021 tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt tcataccgga
    37081 atattccccg gctttacttg tttttttctt tttcaatata aattcacttt tttattataa
    37141 agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc acatcttcac
    37201 ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat ttttgacagc
    37261 gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac aaatgaatag
    37321 tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt tttatgaaaa
    37381 gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa tggtatggga
    37441 tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta taaaagataa
    37501 agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg ttgaaaatgc
    37561 ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt tccacgaagt
    37621 agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc tagacggaaa
    37681 tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg ttttttataa
    37741 caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt tttattcgta
    37801 caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa ttgttagatg
    37861 ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg tatcctttta
    37921 ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga cgtttttaga
    37981 ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc ccttgatgtc
    38041 atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg atttttaagt
    38101 atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga aatgttgtcg
    38161 aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt tcttatcacg
    38221 gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat aaacctgcct
    38281 tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat caattcttgg
    38341 ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag attcatcagg
    38401 aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa aactttagag
    38461 gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta aactgatttt
    38521 ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt tctcactgga
    38581 tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga atatcagttt
    38641 tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct ccaagtacaa
    38701 aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta aagtccgtcg
    38761 tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt tccttaacta
    38821 ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct ggaagaccga
    38881 ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata tcaaatcgtc
    38941 tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg ttttccattg
    39001 aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac gcaaggaata
    39061 cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt tgtttttttg
    39121 tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa gaaaaatatc
    39181 aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc tattggacaa
    39241 aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt attttctaaa
    39301 tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac attcagttcc
    39361 agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat gtctttaccg
    39421 gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag tgtcattaaa
    39481 taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata aaggcagccc
    39541 ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct tgatggtctt
    39601 cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc tgttcgaaac
    39661 gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt tcttctgtca
    39721 aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg aaagcgtcag
    39781 ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc aatgttggat
    39841 catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact tgttcataag
    39901 tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata ccaggaagcg
    39961 tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct cgttccatta
    40021 tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt cttgctttca
    40081 gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga ggtatctttt
    40141 ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc tgctttaata
    40201 tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag atcatctgta
    40261 ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat cacatctttg
    40321 attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac atttccgttt
    40381 tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag tacttcgaaa
    40441 gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc actatctttt
    40501 tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt tgaattgatc
    40561 gttaagacag gatctccttg tctttccgta agagctggta tttctgggag ttctggttcc
    40621 cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac cccaagaagg
    40681 ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga tacgtcagat
    40741 cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt gtctcgatca
    40801 gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga tggaaatgat
    40861 cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg ctagttgttt
    40921 gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa agcaaatcat
    40981 tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt cctttgttcc
    41041 aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa aatggattgt
    41101 agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct tccaatagtt
    41161 ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc gttaacccga
    41221 ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt gtgataaaat
    41281 cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt aagatctggt
    41341 cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa ttataacggt
    41401 cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga tctcctactc
    41461 cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta tttggatctt
    41521 ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa gcagctaaac
    41581 ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa tgtcgtttcg
    41641 ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa aaattttatc
    41701 aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat aaagaaaaag
    41761 agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta atctacataa
    41821 tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt cgtatgcagt
    41881 acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc ttgcgccaat
    41941 tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc tttcagttca
    42001 tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt agttacaggg
    42061 attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact agacagcaaa
    42121 ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga tattccgatg
    42181 tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc tactgtttta
    42241 ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg gtcagtggta
    42301 tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct tgctgcttct
    42361 actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc gtcttctcct
    42421 agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt gggcagattt
    42481 tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc atcgggatat
    42541 actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc atcgcgtaca
    42601 tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat tattttctct
    42661 ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac aatttctatt
    42721 tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag gagccgtttt
    42781 catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt ttaatgcagt
    42841 cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt tagtaaaagg
    42901 tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat cgatgatgat
    42961 tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag caaaaaaggt
    43021 gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt atactccttt
    43081 ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa tctggttgtt
    43141 tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga gagtatcaaa
    43201 agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg ctgttcctct
    43261 ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc tttcttacac
    43321 gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg tttggcattt
    43381 gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta cttaataaaa
    43441 aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat agctttttta
    43501 tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat acgtttcaaa
    43561 ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga tcagtgacca
    43621 atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat ggatgactgt
    43681 acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc ctccagcgat
    43741 gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac tatgcatgac
    43801 atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct gacgattcgg
    43861 caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg tatttgcttc
    43921 tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac ggtcttcttt
    43981 tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct catcctttct
    44041 gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct tctaattgga
    44101 tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact aaagtcggaa
    44161 atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg atataaagat
    44221 gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca tgtgcaatta
    44281 ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac acatccccac
    44341 tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga aacatcgtat
    44401 gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa acgatcaatc
    44461 ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga tcgataaagc
    44521 caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca agatcgccgt
    44581 atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg ctctgctttt
    44641 tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca gtcatatctg
    44701 gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc caaatatggt
    44761 ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa agcaattcgc
    44821 ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct ttttgcagat
    44881 caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg tcttctttac
    44941 gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc atcctttttc
    45001 ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt cttcaagttg
    45061 tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat tatctccttg
    45121 atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa tctcatcttc
    45181 aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag caggttcctt
    45241 gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt gcttcagtaa
    45301 ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga atactaataa
    45361 cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca cagcacctgg
    45421 aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat cattggtcgg
    45481 ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa ataaccggcg
    45541 tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg gaaataaaaa
    45601 tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg cttcttcagc
    45661 agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat tttcttgtgt
    45721 ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca ggggaattct
    45781 gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag taatacctag
    45841 tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt catttatacg
    45901 gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg ctaggttcag
    45961 ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt cgaaacggtt
    46021 gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc aagctaattc
    46081 ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat cacgttctgc
    46141 tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc caaatgaaag
    46201 ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa ccatcaatag
    46261 tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat aatctaaatg
    46321 gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt gcgccagact
    46381 aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt cactgattcc
    46441 taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt ctttattcat
    46501 ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt tttcccctag
    46561 ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt ttccttgaaa
    46621 gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa gataaccaat
    46681 acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat cgataccggc
    46741 aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc cgatacggtc
    46801 tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat aagttttttt
    46861 gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat attgtatcat
    46921 aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt tctcaaaaaa
    46981 agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa cttgaaaatt
    47041 tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg caaatcggaa
    47101 agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga tattcctgcg
    47161 atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat caatgatttt
    47221 tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt gctggatcat
    47281 ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc gttgctggat
    47341 aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac tttcaataat
    47401 caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat cactaataaa
    47461 tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa cgattcgcat
    47521 taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc ttgtgataga
    47581 atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag aaagcttgcc
    47641 cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg cttgtatttc
    47701 ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac gtatccgttc
    47761 ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac gatcaatttc
    47821 ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag cattcaaatc
    47881 cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc ctatgaccaa
    47941 agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt aaaacatcat
    48001 attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc cctttacacc
    48061 gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac tgccatcttt
    48121 gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga cagagattaa
    48181 tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata ctggcgagaa
    48241 cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg taaaagcaat
    48301 agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag agattttcct
    48361 aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag atacatcaaa
    48421 tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca gataccctgt
    48481 gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga accagacaag
    48541 caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc ccgcgtttac
    48601 tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag taacaccaga
    48661 tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag ggatattcac
    48721 tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg ctccaaaagc
    48781 aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac ctaccccgac
    48841 aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat ttttgagaat
    48901 cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa tcataactaa
    48961 gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta ataccactcc
    49021 aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag ctatgttcga
    49081 taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg tccatcccct
    49141 ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta aaattttggt
    49201 ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac gttttgactc
    49261 caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata tatttctttt
    49321 acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat agcgatcatg
    49381 caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct atacacaaac
    49441 ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt catcgtttat
    49501 tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa tagtatcaga
    49561 tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta ctttcccatt
    49621 cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa aatcttgtca
    49681 cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt aataagacac
    49741 atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat tccccgtata
    49801 catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt ttttcgctga
    49861 tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga tatgggaagt
    49921 acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata tcttcgtatt
    49981 tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca tgtgtcgcaa
    50041 ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg atttcaacaa
    50101 attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa agatcgccga
    50161 tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt agcgctgtca
    50221 tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac tacctcagtt
    50281 tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga cgtatctgcc
    50341 cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa atcaattttt
    50401 ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt tctacgacgg
    50461 ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt ccagtttctt
    50521 ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt aaacgatcat
    50581 aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc aattcttcta
    50641 cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa gctccagttt
    50701 ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg ttagaacact
    50761 tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg ctgatcaata
    50821 atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca aaacgtaaaa
    50881 gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt tcaataaatg
    50941 catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc acatttatac
    51001 gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag aaggtttctt
    51061 cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt cgtaaggcat
    51121 cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct tctagatctt
    51181 gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac gcattgatcg
    51241 taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca tgatctggtc
    51301 tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat tgttcatcat
    51361 tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt ggaaaaagct
    51421 gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt ggctggttca
    51481 gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca ttctctaatt
    51541 ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat ttcatttttc
    51601 caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg tgactgtttg
    51661 acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac tcgtccttac
    51721 gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca aaaacttcag
    51781 ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca tcaccaagtt
    51841 cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca taagtgtggt
    51901 tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga gctaatggag
    51961 agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc atactgtata
    52021 aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac tttaacgcaa
    52081 atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg aaggaatttc
    52141 taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg tcaaacgaaa
    52201 aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat ttggtcttgt
    52261 ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca ccttgatctt
    52321 aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca tcccattgat
    52381 tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc taggtacact
    52441 ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca atttacagca
    52501 tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa gcgggatcgt
    52561 ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt tagaacaaaa
    52621 ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct tacttgcttc
    52681 attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt atgttttcag
    52741 tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac ttgtcgtctc
    52801 caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag gtgtgacttt
    52861 cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata tggtcgttag
    52921 ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa aagctttcct
    52981 ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga agccacagac
    53041 aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga aagattccac
    53101 atccagctga cctttgtctt ttttagtact atactatttt taactaattg ttatgttcac
    53161 ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact tcatcagata
    53221 atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga gcataaagca
    53281 tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat tcagtatctt
    53341 cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata gatagatatt
    53401 ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt ttccaattca
    53461 aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc aaaaacaatt
    53521 tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc gcaaaattcg
    53581 ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga taattcaaga
    53641 tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt aaaaaattct
    53701 tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat ctaataatga
    53761 cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt cttgcgctct
    53821 tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat ctggttcatg
    53881 tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt cttctaattc
    53941 attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt ccatctgatt
    54001 gatcacttgt atcacatctt catatctttc atcatccaag taaagattac ttaaagtaag
    54061 taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt cttctgctcg
    54121 ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt cgacttggta
    54181 aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca tttcttcttc
    54241 ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa cttctcgaag
    54301 ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg taaaagctag
    54361 ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag gtacgatagc
    54421 tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca tcgagattcc
    54481 tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg cctctttgaa
    54541 acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt catcaggaag
    54601 caacccagct gcttctttca attttgcttc actcacttca gggattccga taacttggta
    54661 caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat ccactttttc
    54721 caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg ctaacgggat
    54781 gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct gttttgcttc
    54841 gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat ctgctgggtc
    54901 ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat ctgataaagc
    54961 ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca ctgacgattt
    55021 gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg tatccgtcta
    55081 ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa aaaagaactt
    55141 tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag ccaagcaagt
    55201 ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg agattccaca
    55261 cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga tggccatttt
    55321 cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt tctgcctcta
    55381 attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc gttaattgct
    55441 ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa atcattccgc
    55501 aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg ctgattgttt
    55561 gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga tttggtagct
    55621 gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat acagggatca
    55681 tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga tccatgatcc
    55741 cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt ttgacgaaat
    55801 caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa atataccagt
    55861 cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa tcataatagc
    55921 gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc tgactgacta
    55981 gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca agttccatac
    56041 gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag aaaagataag
    56101 agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac atgtgttttc
    56161 tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc tgctaggaaa
    56221 taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg tcttatttaa
    56281 cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct ggaatttcga
    56341 tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga acttcaaagt
    56401 taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga atagttgaaa
    56461 atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct gaagcaactt
    56521 tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca agagatatga
    56581 tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa gaaatattga
    56641 ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca aggtttttac
    56701 caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt ctttttcttt
    56761 tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga taattcggat
    56821 ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac gttcataaga
    56881 aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt tgacagcgac
    56941 ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg gattgattgc
    57001 tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat tctggttcat
    57061 acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca ctgcagaaac
    57121 aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt gttcgaaatc
    57181 acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga taataattcc
    57241 acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa tcccttcttc
    57301 agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg cacgcatcac
    57361 gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag ctgtatctat
    57421 cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt ctcgggtcgt
    57481 tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga tcaaagagga
    57541 tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt cctcttcttc
    57601 agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc ctagtccatg
    57661 gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat agatatcgtt
    57721 tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat tacttctgta
    57781 taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc tgactacaca
    57841 aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct gatccataaa
    57901 aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc gacctaacca
    57961 ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga caatcgagat
    58021 acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc ggccaacgat
    58081 tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct cacttagttt
    58141 aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta tttttattcc
    58201 ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc tgctttcatc
    58261 tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt tcttctactt
    58321 cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt tcttctggta
    58381 attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct tctaaagctt
    58441 tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc actttcactt
    58501 cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa atttgagaga
    58561 tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag cttgtcaaac
    58621 gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt tctgaccaag
    58681 gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga ttgatagaca
    58741 acactttgac atttacatca tcattgacag ccaatacatc acttggtttt gctacatgac
    58801 tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg acaaatgcgc
    58861 caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg atagttgaaa
    58921 gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc gataagatca
    58981 aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg cctttatagt
    59041 ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa ccacgtacac
    59101 cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg atgaccttgc
    59161 cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa cggcgttttg
    59221 aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg actaaatcta
    59281 atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt gataattctt
    59341 ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact tgtttgtctt
    59401 caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt acgcttttca
    59461 ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc ataacacttg
    59521 tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat tgattattca
    59581 aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt catttttttt
    59641 cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg ctgcaacgac
    59701 ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt gcttcagcgg
    59761 agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg tcagtgtttc
    59821 aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg cacgctcact
    59881 tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg tcccgatgtc
    59941 tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag caactaattc
    60001 ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat ctggttgacg
    60061 gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt cttgttgcga
    60121 aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg aaaaagatac
    60181 attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat ctgtataaat
    60241 ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg aagcaggccc
    60301 gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa aaaaagccgc
    60361 aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca taaagttgtt
    60421 tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac gctcagccac
    60481 ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag ctgatgaaga
    60541 aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg cctcacttga
    60601 ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg tatcttgaga
    60661 cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag ttgactcctc
    60721 ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag aagttgctgc
    60781 tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg gaatagcgat
    60841 acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt tcttttgttg
    60901 tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt gttcccatgg
    60961 ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca ctgttttacc
    61021 tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct aaaaaatcaa
    61081 tgttccatgt tttttttaaa taaatttagt aaaatcgttt cccaatgaag ttgttcagtc
    61141 ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat atcacaacaa
    61201 ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt tctgcggcag
    61261 ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc tttttcctga
    61321 cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag ctccatccat
    61381 tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata aagctcaaaa
    61441 ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc atccccactt
    61501 tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc gatttcttgt
    61561 acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat atcagattta
    61621 tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt ttttacaaac
    61681 tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta tccaactgta
    61741 aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc acaataaatg
    61801 attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt ttgctgcgtt
    61861 ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc ttttggtggt
    61921 cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt cgccgttagt
    61981 gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg atattctgga
    62041 cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata taacgcaata
    62101 ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg actcaaaaac
    62161 aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg cgaaagtaaa
    62221 ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac ttgatcttcc
    62281 attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata ccccgtcaat
    62341 tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc ttctccatca
    62401 agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc aaaatatttt
    62461 tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga tttgatacaa
    62521 acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc gccactctcg
    62581 cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa tccactcgta
    62641 tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg ttcctctttt
    62701 taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca tctgcttggt
    62761 gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag cgtgccaaag
    62821 cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc tttgttgcag
    62881 ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc ctgttcgctc
    62941 atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct ccgaaaacag
    63001 ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt ggctttcact
    63061 aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa gatagtcttg
    63121 aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc gccaactagt
    63181 ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt gatgttcgga
    63241 caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt cgatccaacc
    63301 ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt ctggaaaact
    63361 gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag tcgttcgttt
    63421 tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg aaaatagcga
    63481 taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat gtcactacta
    63541 tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga agcgccagat
    63601 attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga ggctcatcaa
    63661 cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa atgtccagtt
    63721 cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa acgtacacta
    63781 agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga aatcccgact
    63841 tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca cggaccctat
    63901 aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt gacgatcaca
    63961 gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc accatagctg
    64021 ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc cggtaaatac
    64081 tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc cgtgatcgat
    64141 aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact tgccatgaag
    64201 aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact tgccattacg
    64261 ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc tttgacctta
    64321 tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat agcgactgaa
    64381 aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc tacgagagca
    64441 ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc ttcaaaaaat
    64501 aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga cgatggttcg
    64561 tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa aatgatgact
    64621 aagccaatta gtattcgtct aaatattttc actttctccc accttttcta tctgagtgac
    64681 ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa tgcaaacttt
    64741 ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt ttgtatgttt
    64801 atttttattt agcacataaa cgatacacag cttctgcata aatttccatt gctttataca
    64861 tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta tcagggaaca
    64921 tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg cctgatacaa
    64981 acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt ttcactaact
    65041 cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa gtcaacccat
    65101 atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat gtaacaggga
    65161 ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt gcaaagttca
    65221 tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca ccagttgcat
    65281 tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact tctgaaagag
    65341 caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg tggatacttt
    65401 taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt tttttcaatg
    65461 cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga acaacattca
    65521 acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag taagcttgca
    65581 ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat ccttgcgtaa
    65641 ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct tcatctgtac
    65701 caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa gctttgacgg
    65761 catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca actaaccagc
    65821 cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc cctgctggaa
    65881 caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc tctgaataac
    65941 cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg atttccaata
    66001 ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct gaatctgctt
    66061 catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct tgttcatgat
    66121 gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa aagggtcatg
    66181 tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc ctgctccatc
    66241 agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa tggagctatt
    66301 gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta tgatagacct
    66361 tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca cacagatggc
    66421 tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa catcgaaacg
    66481 agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt ttactaaacc
    66541 agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa attcacccat
    66601 aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa tcaagaatga
    66661 catagggata tagaaaataa atgtaataat gatgaataca cttgaagata atgaacttaa
    66721 tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca taaccacttg
    66781 gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag caccagctaa
    66841 aaattcagag acaaattcag actctttcat gccataagtg attgctacta cgatggacat
    66901 tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa gcatgctttt
    66961 acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg attcaaaaat
    67021 cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga taccaaaagt
    67081 aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt gacgaccgat
    67141 tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta atgattttgt
    67201 tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa tagctaacaa
    67261 aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc caacagcttg
    67321 tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta cttgagaccc
    67381 tactaatacg attgctacag ctactactgt atcaaagcct acagccatca tgactgggat
    67441 cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc cgcctaatgc
    67501 aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt tcttcacaac
    67561 tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa atccaccaac
    67621 aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta gctcccccgc
    67681 tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc cttgaggatt
    67741 ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta cactatagtg
    67801 cccagctggt ataaaccaag taagtacagc gattactgca ataataataa ataagaccgt
    67861 atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct tcttcctttc
    67921 ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag agtatcagaa
    67981 caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt ttccctattc
    68041 cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt ttcctttttt
    68101 ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc aaagaattga
    68161 ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac gtttacatta
    68221 ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga tctttttgat
    68281 atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact taaccatgga
    68341 agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt gatcaagttg
    68401 aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat ctgtgttgcg
    68461 ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc gatactcata
    68521 aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct tcctttatgc
    68581 tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt tgataagaaa
    68641 ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag atgtaatact
    68701 gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat gctgatcaat
    68761 accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat cggaaaagca
    68821 aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat tttctgtaca
    68881 cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac tttttgacgt
    68941 ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt tatttgatca
    69001 agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta tcggaatttc
    69061 accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg tcgggaattt
    69121 caccctgccc ctgaagacga accttatata atttttgttt acatattcat tatacatatc
    69181 tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt gagtggttca
    69241 gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt aggccttgta
    69301 aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca actgcttgaa
    69361 gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg atactgtggt
    69421 ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt cgtccttcga
    69481 tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct tttacctttg
    69541 caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca aaatctccat
    69601 catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca ggatagatac
    69661 gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca tctgcaacag
    69721 cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct tgataaattg
    69781 ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat tcccttacaa
    69841 cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt cgacgagaag
    69901 tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt ctcctttatt
    69961 cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt ccatttcttg
    70021 tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt agccagttgt
    70081 tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa tcagtttatg
    70141 ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct cgatttcagc
    70201 tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg cttgagataa
    70261 tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt ttttagtcgt
    70321 taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt tttgagtgta
    70381 agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat ttaacagtcc
    70441 gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg cttcaagttc
    70501 acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt gattcatagt
    70561 tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa gccataaaag
    70621 tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta cagccatgcg
    70681 catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt gtgtcatcgg
    70741 gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt ttctgcatgg
    70801 cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca gcatccgcta
    70861 acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct gctttttctg
    70921 tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg accaattctt
    70981 ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg acaggaagca
    71041 atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct cctgcaacag
    71101 ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg atcgtagcga
    71161 tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc agatcgagag
    71221 gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact ctttcttctt
    71281 tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac ggatggagac
    71341 atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct caccgcgata
    71401 agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc agtaaatacc
    71461 aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt cataatcgtc
    71521 ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca gtttcttatg
    71581 gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg ttaacatctt
    71641 ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg tcattctttt
    71701 cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta acaattcctg
    71761 tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat gccgcagcgt
    71821 atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca gatttttcca
    71881 gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca tttcagatgc
    71941 gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt ggatcgcata
    72001 atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta ccagtccaag
    72061 agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca tcccagtagc
    72121 atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg tatcaggtgc
    72181 ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct gtgctttctt
    72241 cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc gtaaaaactg
    72301 atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt ttttttcttc
    72361 atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc atgaagcaat
    72421 tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc tttgtcgtgt
    72481 attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga tttgctcttt
    72541 cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc tgatagtctt
    72601 tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg gttt
//
LOCUS       DACDLI010000004        72434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDLI010000004 DACDLI010000000
VERSION     DACDLI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.004"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(160..444)
                     /locus_tag="IV353_000269"
     CDS             complement(160..444)
                     /locus_tag="IV353_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008550.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="IV353_000270"
     CDS             complement(462..1352)
                     /locus_tag="IV353_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9008551.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="IV353_000271"
     CDS             complement(1372..1590)
                     /locus_tag="IV353_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008552.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="IV353_000272"
     CDS             complement(1560..3260)
                     /locus_tag="IV353_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9008553.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="IV353_000273"
     CDS             3527..4300
                     /locus_tag="IV353_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008554.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IV353_000274"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IV353_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9008555.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="IV353_000275"
     CDS             complement(5124..6095)
                     /locus_tag="IV353_000275"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9008556.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IV353_000276"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IV353_000276"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9008557.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IV353_000277"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IV353_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9008558.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="IV353_000278"
     CDS             complement(9050..9874)
                     /locus_tag="IV353_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9008559.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="IV353_000279"
     CDS             complement(9847..10527)
                     /locus_tag="IV353_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9008560.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="IV353_000280"
     CDS             complement(10550..11488)
                     /locus_tag="IV353_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9008561.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12584)
                     /locus_tag="IV353_000281"
     CDS             complement(11733..12584)
                     /locus_tag="IV353_000281"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9008562.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IV353_000282"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IV353_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9008563.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="IV353_000283"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="IV353_000283"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9008564.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IV353_000284"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IV353_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9008565.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IV353_000285"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IV353_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9008566.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="IV353_000286"
     CDS             complement(19462..20703)
                     /locus_tag="IV353_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9008567.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IV353_000287"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IV353_000287"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9008568.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22614)
                     /locus_tag="IV353_000288"
     CDS             complement(21730..22614)
                     /locus_tag="IV353_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9008569.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="IV353_000289"
     CDS             complement(23392..24678)
                     /locus_tag="IV353_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9008570.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="IV353_000290"
     CDS             complement(24671..25939)
                     /locus_tag="IV353_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9008571.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="IV353_000291"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="IV353_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9008572.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="IV353_000292"
     CDS             complement(26711..27157)
                     /locus_tag="IV353_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9008573.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27250..27495)
                     /locus_tag="IV353_000293"
     CDS             complement(27250..27495)
                     /locus_tag="IV353_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008574.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27610..28188)
                     /locus_tag="IV353_000294"
     CDS             complement(27610..28188)
                     /locus_tag="IV353_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9008575.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28265..28795)
                     /locus_tag="IV353_000295"
     CDS             complement(28265..28795)
                     /locus_tag="IV353_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9008576.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="IV353_000296"
     CDS             complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="IV353_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9008577.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29652..30047)
                     /locus_tag="IV353_000297"
     CDS             complement(29652..30047)
                     /locus_tag="IV353_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9008578.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30044..31456)
                     /locus_tag="IV353_000298"
     CDS             complement(30044..31456)
                     /locus_tag="IV353_000298"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9008579.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="IV353_000299"
     CDS             complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="IV353_000299"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9008580.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32311..33771)
                     /locus_tag="IV353_000300"
     CDS             complement(32311..33771)
                     /locus_tag="IV353_000300"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9008581.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33941..35098)
                     /locus_tag="IV353_000301"
     CDS             complement(33941..35098)
                     /locus_tag="IV353_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9008582.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35132..36502)
                     /gene="radA"
                     /locus_tag="IV353_000302"
     CDS             complement(35132..36502)
                     /gene="radA"
                     /locus_tag="IV353_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9008583.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36565..37092)
                     /locus_tag="IV353_000303"
     CDS             complement(36565..37092)
                     /locus_tag="IV353_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9008584.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37322..38128
                     /gene="proB"
                     /locus_tag="IV353_000304"
     CDS             37322..38128
                     /gene="proB"
                     /locus_tag="IV353_000304"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9008585.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38125..39372
                     /locus_tag="IV353_000305"
     CDS             38125..39372
                     /locus_tag="IV353_000305"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9008586.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39448..39792
                     /locus_tag="IV353_000306"
     CDS             39448..39792
                     /locus_tag="IV353_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008587.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39909..40235)
                     /locus_tag="IV353_000307"
     CDS             complement(39909..40235)
                     /locus_tag="IV353_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008588.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40608..41809)
                     /locus_tag="IV353_000308"
                     /pseudo
     CDS             complement(40608..41809)
                     /locus_tag="IV353_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41926..42441
                     /locus_tag="IV353_000309"
     CDS             41926..42441
                     /locus_tag="IV353_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9008589.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42640..43086)
                     /locus_tag="IV353_000310"
     CDS             complement(42640..43086)
                     /locus_tag="IV353_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9008590.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43133..44611)
                     /gene="msr"
                     /locus_tag="IV353_000311"
     CDS             complement(43133..44611)
                     /gene="msr"
                     /locus_tag="IV353_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9008591.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44965..45594)
                     /locus_tag="IV353_000312"
     CDS             complement(44965..45594)
                     /locus_tag="IV353_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9008592.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45578..46042)
                     /locus_tag="IV353_000313"
     CDS             complement(45578..46042)
                     /locus_tag="IV353_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9008593.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46529..47797)
                     /locus_tag="IV353_000314"
     CDS             complement(46529..47797)
                     /locus_tag="IV353_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9008594.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48028..48765)
                     /locus_tag="IV353_000315"
     CDS             complement(48028..48765)
                     /locus_tag="IV353_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9008595.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48832..49401)
                     /locus_tag="IV353_000316"
     CDS             complement(48832..49401)
                     /locus_tag="IV353_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9008596.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49529..50401
                     /locus_tag="IV353_000317"
     CDS             49529..50401
                     /locus_tag="IV353_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9008597.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50743..51615
                     /locus_tag="IV353_000318"
     CDS             50743..51615
                     /locus_tag="IV353_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9008598.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51612..53570
                     /locus_tag="IV353_000319"
     CDS             51612..53570
                     /locus_tag="IV353_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9008599.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53821..54672)
                     /locus_tag="IV353_000320"
     CDS             complement(53821..54672)
                     /locus_tag="IV353_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9008600.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54795..55262
                     /locus_tag="IV353_000321"
     CDS             54795..55262
                     /locus_tag="IV353_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9008601.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55525..56007)
                     /locus_tag="IV353_000322"
     CDS             complement(55525..56007)
                     /locus_tag="IV353_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9008602.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56107..56970)
                     /locus_tag="IV353_000323"
     CDS             complement(56107..56970)
                     /locus_tag="IV353_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9008603.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57568..58416
                     /locus_tag="IV353_000324"
     CDS             57568..58416
                     /locus_tag="IV353_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008604.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58587..58928)
                     /locus_tag="IV353_000325"
     CDS             complement(58587..58928)
                     /locus_tag="IV353_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP9008605.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59014..59721
                     /locus_tag="IV353_000326"
     CDS             59014..59721
                     /locus_tag="IV353_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9008606.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59708..60031
                     /locus_tag="IV353_000327"
     CDS             59708..60031
                     /locus_tag="IV353_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9008607.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60120..61157)
                     /locus_tag="IV353_000328"
     CDS             complement(60120..61157)
                     /locus_tag="IV353_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9008608.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61604..62587)
                     /locus_tag="IV353_000329"
     CDS             complement(61604..62587)
                     /locus_tag="IV353_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9008609.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(63003..63773)
                     /locus_tag="IV353_000330"
     CDS             complement(63003..63773)
                     /locus_tag="IV353_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9008610.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63766..64701)
                     /locus_tag="IV353_000331"
     CDS             complement(63766..64701)
                     /locus_tag="IV353_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008611.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65295..66944)
                     /locus_tag="IV353_000332"
     CDS             complement(65295..66944)
                     /locus_tag="IV353_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9008612.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(67139..68035)
                     /locus_tag="IV353_000333"
     CDS             complement(67139..68035)
                     /locus_tag="IV353_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9008613.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(68028..68708)
                     /locus_tag="IV353_000334"
     CDS             complement(68028..68708)
                     /locus_tag="IV353_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9008614.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(68709..69491)
                     /locus_tag="IV353_000335"
     CDS             complement(68709..69491)
                     /locus_tag="IV353_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9008615.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(69484..70401)
                     /locus_tag="IV353_000336"
     CDS             complement(69484..70401)
                     /locus_tag="IV353_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9008616.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(70404..70688)
                     /locus_tag="IV353_000337"
     CDS             complement(70404..70688)
                     /locus_tag="IV353_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9008617.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(71389..72009)
                     /locus_tag="IV353_000338"
     CDS             complement(71389..72009)
                     /locus_tag="IV353_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008618.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(72090..>72434)
                     /locus_tag="IV353_000339"
     CDS             complement(72090..>72434)
                     /locus_tag="IV353_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008619.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDNNDQYLPKK"
BASE COUNT        20830 a        15442 c        12751 g        23411 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    23041 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    23101 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    23161 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    23221 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    23281 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    23341 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    27241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    27301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    27361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    27421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    27481 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    27541 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    27601 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    27661 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    27721 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    27781 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    27841 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    27901 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    27961 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    28021 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    28081 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    28141 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    28201 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    28261 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    28321 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    28381 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    28441 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    28501 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    28561 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    28621 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    28681 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    28741 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    28801 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    28861 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    28921 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    28981 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    29041 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    29101 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    29161 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    29221 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    29281 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    29341 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    29401 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    29461 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    29521 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    29581 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    29641 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    29701 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    29761 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    29821 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    29881 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    29941 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    30001 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    30061 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    30121 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    30181 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    30241 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    30301 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    30361 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    30421 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    30481 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    30541 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    30601 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    30661 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    30721 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    30781 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    30841 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    30901 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    30961 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    31021 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    31081 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    31141 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    31201 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    31261 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    31321 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    31381 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    31441 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    31501 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    31561 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    31621 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    31681 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    31741 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    31801 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    31861 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    31921 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    31981 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    32041 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    32101 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    32161 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    32221 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    32281 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    32341 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    32401 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    32461 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    32521 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    32581 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    32641 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    32701 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    32761 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    32821 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    32881 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    32941 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    33001 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    33061 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    33121 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    33181 attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga tattatcgga
    33241 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    33301 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    33361 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    33421 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    33481 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    33541 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    33601 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    33661 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    33721 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    33781 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    33841 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    33901 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    33961 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    34021 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    34081 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    34141 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    34201 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    34261 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    34321 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    34381 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    34441 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    34501 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    34561 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    34621 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    34681 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    34741 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    34801 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    34861 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    34921 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    34981 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    35041 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    35101 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    35161 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    35221 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    35281 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    35341 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    35401 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    35461 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    35521 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    35581 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    35641 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    35701 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    35761 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    35821 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    35881 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    35941 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    36001 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    36061 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    36121 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    36181 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    36241 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    36301 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    36361 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    36421 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    36481 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    36541 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    36601 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    36661 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    36721 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    36781 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    36841 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    36901 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    36961 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    37021 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    37081 tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc
    37141 tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta
    37201 attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt
    37261 actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca
    37321 catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt
    37381 gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc
    37441 tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg
    37501 tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc
    37561 ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca
    37621 acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa
    37681 tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa
    37741 cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct
    37801 gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga
    37861 cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga
    37921 taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg
    37981 cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat
    38041 tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg
    38101 tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa
    38161 gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa
    38221 atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta
    38281 gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct
    38341 gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt
    38401 ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg
    38461 ccactaggcg tgaccggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct
    38521 agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc
    38581 tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct
    38641 tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg
    38701 acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa
    38761 gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat
    38821 aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca
    38881 tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg
    38941 ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg
    39001 ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta
    39061 gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat
    39121 cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt
    39181 ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac
    39241 ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt
    39301 ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa
    39361 atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag
    39421 gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac
    39481 cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa
    39541 ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc
    39601 gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta
    39661 gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg
    39721 attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg
    39781 actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact
    39841 gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg
    39901 gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg
    39961 tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt
    40021 tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca
    40081 gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct
    40141 ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt
    40201 gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt
    40261 aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg
    40321 gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa
    40381 ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc
    40441 cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaaaagct
    40501 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct
    40561 gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta ttcttcttca
    40621 tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag aacaatggca
    40681 cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc tgcaataaat
    40741 ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc aacgtttcgt
    40801 tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat tccttggaaa
    40861 aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc agctcctaat
    40921 aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata agtcgtaaac
    40981 ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac accccagcca
    41041 aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg cgcatacgca
    41101 agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga acgaagttta
    41161 tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg tttctcagtt
    41221 tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa
    41281 aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc aacagccatt
    41341 ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc aactaaaata
    41401 ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc ccagccggca
    41461 cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc gaatacaacg
    41521 atgataaaca atcctgtcat caatgttttt tcaaaccgat ccgactagat aagaaacctg
    41581 taaatagcat caccacacca gtgactaaca tgtaactagt aaataacagg gtcgtttcag
    41641 ctggcgtagc gtttaactgt gcagcaatcg tttttaagat tgggtctact agacccactc
    41701 ccataaaagc gatcatacag gtagctgcta caactagcgc tgaacttttc ccctttgttt
    41761 cttcatttgt ttgttcttgt atatctattt gattctccaa tatttccatc cctttctctc
    41821 tattagcatc atacatgatt tattttacat gtaaatatgc acatgtaaaa cgtggaaact
    41881 ttactgtgat ataataaaga aaataagacg agaggtagga tttttatgaa tactttagcc
    41941 tatatattat tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt gaagcagctg
    42001 atcacaattt tttgggaagc ccatcacagt cagatctata caacgcttgc caagttagac
    42061 cagcagggtt atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa gatctaccac
    42121 ttgactgatg aaggacgtgc tcttgtagag gaatggatca aagaggaaac tcccgctcct
    42181 acgcaaaaag atgaatttct agcaaaaatc tatgcttttt cgacgctcga taaaacaaca
    42241 gctactggac tgctattcac tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa
    42301 aaaaaattga atgacctctc gctagacaag aaacaagaat ttggacgtta tgttgtgatc
    42361 cagcgcagaa ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa
    42421 atgcagcagt tttttgagta agggataaac agaacctttc tagaaatatc tattgaagag
    42481 caaattatct tcaataaaag agcaatacag ttggaaaaga aaaagcgatc cgaacgacag
    42541 gccaaaacct atcgttcgga tcactatttt tctagaactg cattcaagag actatgacaa
    42601 aaagcatgcc aaggcctttt ttcaaataac agtgttccat cattcattac tttgaattgc
    42661 ttttagcaca cttcttgaaa gcgggccaac gatgatcaac tgcaacggta aagcggcaat
    42721 cacattcatt ttccaagcat tcatataagc agagaataat tgatcaggta ttccttgttc
    42781 catgatcaca ccgaataaag acatacatgt caccattcct aaaaccatca agcatgaaat
    42841 agttaagatc atttggacct tgctttctgg atttactggt agtttaaagg caattttttt
    42901 cgccagcaca ccgatgatga agacatccaa aatgaaagcc accacgaatc caggcactag
    42961 accaatcaac aaacttgata aatgaagatc ttgatgcagc cataaattat acgcgctcat
    43021 ccccaagacc atcaaaaaac acatcattgt tgtaaaaatt attccttctt ttttatttgt
    43081 aggcaatctg taccactcct ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat
    43141 ttctcgtaag tacttttttt attttctgcc cttttatttc ataaacttca tcagccgtac
    43201 gctcgacaaa gtaagaatca tgagaagtaa acaaaatcgt tcccggatat atttgcatta
    43261 gcttctctaa agcttcgatc gttgccatat caataaaatt agtcggttca tctaacagca
    43321 acacattact tggcttagta aaaagcaaag caatcgtcag acgggtcgct tctcccccac
    43381 ttaatgtaca taacggtttc gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac
    43441 tacggacaag cgattctgaa aactctgttt gtctcatgac aaaacgaatg atggattctt
    43501 cagatgtcat ttgataaccc ttttgctgat atacttgaaa gcttactttc ggagagagaa
    43561 ggattccttc tccttgtttg cggatatgtt ctaataaggt tgtcttacct gaaccatttt
    43621 caccgacaat cgcgattttt ttacctaaag aaaactgaaa atcaccattt accagtaatg
    43681 ttctcccact tctttccaat tggacatttt gtcccatgat gggatagcgg ctgtggagtt
    43741 caagagattt tggtactgga aaacggattt gtttcatctg ctgcggcttg gttgtttctc
    43801 ctattttttg gagtcttctc tctaatgttt tcgcattctt ttggatggct ttttgtacac
    43861 tgtctttttg tttagaggaa gacaaccgat ccggtctgat ttgttgcttt ttctttttac
    43921 tggacacttt ttccaacttt tccgcttgtt ttttcttttc ttggatcgat ttttccaatc
    43981 gttttttctc tttcatgatc gactctgctt ctcgtagctg agtctttttc tccaattcct
    44041 tttgagtgag ataggcacta taattccccg aaaactctcg gatttctccg tcttttactt
    44101 cccagatttt cgatgctagc tcatctagaa aatagcgatc atgactaaca aaaaccagcg
    44161 taccataata ataacgaagc tcttcaatca gtcgatcaat actttttcta tctagatggg
    44221 ttgttggctc gtctagaagc atcccttgtt cataagaaga aagaagacgt atcaactcga
    44281 tctttcgttc ttcccctccg ctccatttcc gctctgaagt ttcttccagt cttaactgga
    44341 aatgactggc taattccaaa tttaggtcct ttgcttcagc gagataggtt aattgaggca
    44401 ggtagttgaa ggtgatctct gtttggattt tcccatgatc gggaaaaagt tcaccggcta
    44461 ttagtttgag caaagtcgat ttaccgacac cattttctcc tatgatccct atacgatctt
    44521 gctgatagac acgaagagaa tcaatagata gttctagctg gttgccaaag ctgacttgta
    44581 gatttgttat atttactgct aaattttcca tatgttttac actcctattt catgtaaaac
    44641 aacaattatc gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat
    44701 cttttcctac tctgaacgaa ataactatga ctaaatcacc aaatctgtca tagctttcac
    44761 ctattctact gtcgttcgaa aaaatggaca tactccatcc atgaaattgt tgtttataca
    44821 tgtcttttga atttatttag ttgttattca aaagttcgaa acgtaatttc atcgatgcag
    44881 tcatttttcc gacctccttt tttattaggt taccacacac gattcaaaaa aagcaatagc
    44941 tactgaatag taactgaaaa ccttttattc gaacgtagtg atcttttctg gtgctaaacg
    45001 aacagactcg taaccttctt ctaccgcttt tcctattgat aaaataataa ctggaacata
    45061 acggtcttta tccaaaccaa atgcctctgc caactgatct gcttcgaatc caccaattgg
    45121 atttgtttca tagccatgat cacgcgcaac taacatgaat tgcatagccg ctaagctaga
    45181 atcgactttg actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat
    45241 cgcagctaat tgttgatctc tgacttcttt tggcatcttc cctgattcgt aagcttggtt
    45301 atagatttct tcgccatatt cgtaacattg catatcgcca aaaatcatga ccattgcaga
    45361 agaagtatca ttttgtctcg tattgaaacg gatcaaagga cgcaagattt cttttgcttc
    45421 ctcgctttca gcgaccacaa agcgccatgg ttgcatattg acagaagaag gtgcagtcgt
    45481 cgcttctttg atcatttcca gcatttcttc gtgtgggatt ttgtatgttt catcatatac
    45541 gcgaacggat cttctaccag tcataattgt tgaaaaatca tttttttgtt ccattattat
    45601 taacctcttt cgtttctgtt gttatattgc aaagttgggt attcaattta tctaaaagtg
    45661 ttagaaattg tttcatttct acatcgctga aagccgggga tagcacggaa cctgtatctt
    45721 tatggtccat ttcacaatgc tcgattttct tctgggcttt ttctgttagc gctacataca
    45781 cttcccggtt gttctgagga tttctctgac gcgaaacata gccatttgct tccaatacct
    45841 tcagatgacg agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta
    45901 gcaatggatt ttcttctttc agtctcatca tgatctcgta tctagtaaga cttataccga
    45961 tattttcttc aaagatctgg acgatttttt gatcggctaa tttgatttga tataaaaggt
    46021 gactaatctc ttctaaagac ataatttcct cctttctaat aattgactcg tcaacgattg
    46081 acttgtcaat aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa
    46141 aacgctctca atagaaaagt atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat
    46201 gataaagaaa gcacaacgct aaaaccaaga ttattgattc ttatcaaact atagaagata
    46261 gctcttctgc agtagaaaat tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga
    46321 atcccgtcga atattagaaa ttggatcagt atgcttaaac agtaataaaa aagttatgac
    46381 aagatgttgt cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat
    46441 aagttcaacc aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct tactacttga
    46501 tgcagggcag ctttttcact acctcttttt aataaccata aaatcctatt ccttcatatc
    46561 tccagcccat cggtgttagc caatctcttt ccttagcact caaggtatag tgatgggtac
    46621 cagttttttc atttggatta aatagtcgat aaattggctt ggaaccacca gaataaaaac
    46681 ttagaccttc ataacgccaa ccgatttttg ttaaagagtt gatctcagat tgtgccattg
    46741 tgtaatgatg gtctccactg taaggattgt ataaacggta gacaggttgt ccgctgtttg
    46801 gtgcttgcca agcaatccct tcatattccc agcctacagt tggcagataa tttttttctc
    46861 cggcattcaa tgtatataaa tgttctcctg tatgacggtt atacagacga tacatcggag
    46921 aagttgaagt tggtgctgta tcatactgtg tcagattata ggtttcgatt acattgttca
    46981 atgtagaacc ataattaggc gcagtggcat aacgacctgt tagccaagcc gtagcatctt
    47041 tgtaagagtt cgtattgctt ttccatgcac cggaatagta gtaaacgcct ggctgaaaag
    47101 aagtcgtctt caataccgtt acgttatctt gcaatgattc tttgtacgaa ggatattttc
    47161 taaaagcggc attcaccgta atccattgcc cattcacata ttccgaagta ggcatcgtaa
    47221 cagattggcc attatagctt cctttaatcc caaatagatt gtaattaggg gcagaagcca
    47281 aagtactgtt cccccagcca ctttcaacga tggcttgagc aatcatgact gatgcatata
    47341 agtcattggc acttgccaaa ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt
    47401 gagaattagc agatgagcga gcataaaaag catagccagt atagttttcg aattcagaca
    47461 aatctgcttg atgggtatgt gtcaatgttt gcccatctgt caattggttc gtgatttctt
    47521 ctgcattctc tggattgttc tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt
    47581 ctgttgtttc agttgttggt tcttcggttg aaattgtatt ttctgagaca gtagctgact
    47641 gcaaagtact ctctgtatcg ttttcaattc ctgtttctga agaactcgat gtctgagttg
    47701 cgacagtttc tgtcgattct tcagctagca ctgttgtgtt ggcaactgct agatttccta
    47761 aaagaataac gctaaataat aaattctttg ctttcatctt ttttctcctt tgaattatga
    47821 aacttttcct aaataacagt gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa
    47881 ataacatttg tttctttttg ataaatttgc tttttctaca taaaaagacg taaatgaaga
    47941 atattcttct ccattcacgc cttaaattga tggtcgtttg aataactatt taacaagtaa
    48001 ttctctttgg atattatccg ttatggatta tacttcctgt gtcgtcggac ggataatcat
    48061 gttactgaca gacatccggt ctggcgtttc tatcgcaaat gctacagcag aagctatatc
    48121 ttcggaggtc aatccccatt ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc
    48181 gctgatggtt tgatataatt cagtttgaac agctcctgga gagatgatcg tagatttgat
    48241 gttattttgc cgttgctctt gacgtaatcc ttccatgatt gcacggacag caaattttgt
    48301 tccgcaataa acggcggaat caggataaac aacatgtccc gccacagaat cagttgaaat
    48361 gatatgccca gatttttgtt tttccatgat tggaagtacg gcggaaattc catttaacac
    48421 acccatgatg ttgatatcaa gcatattttg ccattcatca cggtgacctt caactaatgg
    48481 agcggttggc atgattcctg cattattgta caatacatcg attcgaccga acttttccat
    48541 cgctaaatcg ataactgctt ggacttgtgc aaaatctctt acatctgctg tttgaatata
    48601 tagctcagct tctggtaaag attcttttat tgcttttagg cgatcttctc ggcgtgcagc
    48661 tataactagt ttagcccctt tttctgctaa tagcttcgtt gttgcttcac cgattccgct
    48721 tgaagcgccc atgatgacaa tcactttttc tgataaatca ttcattctat tctcctcctg
    48781 ttttgaaaaa tctcttgctt atactcacta tctcttattt catcttactg ttcaaataat
    48841 ccgttcgaca ggtttgacga ttttggcagg gattcctgca acgatggtat tgtctgggac
    48901 atctttcgta acagttgcat tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg
    48961 cagaatagtt gctcctgctc cgatccaagc atttttcttg atgtggattg gctttactga
    49021 caagcctctt cgttcttttg gatcggttag atggttcacc gtgatgaggt tgacacgagg
    49081 accaatcaat actgaatctt ctaaacagat gccacctaag tcaacgaaca tcacttgacg
    49141 attgataaag acatttttcc ctatactaat atgtttgccg aaatctgtat aaaaaggcag
    49201 cagaacagtc accgtatcat ctactttgtc actaatgata ttttctagcc gtttgcggat
    49261 atctgctggt tcatggtacc cagtattcag cgctgctaaa ggctcttcgt tttcagcttg
    49321 gatctgatgg atcacttcaa agagcggatc cgttactcga atctctttat tgacgatttg
    49381 tttttcgatg aggcttgtca ttttgctccc tccttttatc cttactttat aacggacggc
    49441 ttatcatgtc taatacttat aaaacatact taactattcc ttttaactat acttagtggt
    49501 atgctttttt aaaaacagga gggaaaaaat ggatattcgt atactgacgt attttttagc
    49561 agtagctaga gaaaaaaata tcagccgagc agcaaaatct ctgcatatca cgcaacctac
    49621 tctctcaaaa caactgaaag atttagaaga agaacttggt gtgacacttt tcactcgggg
    49681 ctcccgagag atacagttga cggattctgg tcgatatctt tattcaaaag gaaaagaaat
    49741 cttgtcgttg gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca tctctggaga
    49801 aatctacatt ggcggaggcg aaactaaagc aatctctttc atttctgata gccttcattc
    49861 gttgatcaag gcgcatcctg atatccagtt tcatttatat agcgggaatg cagatgatat
    49921 catagacaaa ctggatagcg gattacttga ttttggactc gtaatcgaac cgacaaacaa
    49981 acaaaaatac cagtcatttc gactgccagc ccaagatcgt tggggacttt tgatacatcg
    50041 aaatcatcct ttagcaaagc aaaccgtcat ccatccgcag gaactgaaaa ctgttccttt
    50101 gatcatttcc cggcaaacgt cagtggacag tcaactagct gaatggctag gtttcagcat
    50161 agatcagtta tcgatcgtcg gaacatataa cttattgtac aatgcttctt tgatggtcga
    50221 taacgggact tttggtgcat tagctctaga tgggatcatc aatacttatg ggacggaaaa
    50281 aatattcatc ccactcgatc cgccattaca agcttcactg aatctcattt ggaaaaaaga
    50341 ccagcctctt tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga aatacagtta
    50401 agtagagttt ttttccttcg ctttcagctt tttaacaacg aataagataa gcaaaagatc
    50461 caacgattaa ataacataag tttcttaatt tatttcttcc ttttatttct gataattcca
    50521 gtaaattact ataattataa aaaaagaatt gacaaaacat aactgtctat ataaaattga
    50581 ttacgttaac aataattcgt ttcatgtgac tagatcatac taggcatgaa aaatctctga
    50641 ggaacatggt ttccctagga ggtttttcgt gtctattttt gtttggccgc aatcaactgg
    50701 tctagcaaac gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc
    50761 tttaatcaaa atgcagtatt ggtagatgac ggtggacagg aaaaagtagc tattggaaaa
    50821 gggattggat ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt cgaacggata
    50881 tttgtcatgg aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa
    50941 cgctttttat ttgcagcaga aacgatcatt gatcatgcgg aaactgtttt gatggaaaag
    51001 ttgaatgaac atgtattgat cgctttgacc gaccacattg ctttttctgc ggagaacatt
    51061 aataacggta tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct ttacagtgat
    51121 gaattcagta ttgctcaatg ggcagtagag tatctgacaa aagagcttgg gattccatat
    51181 acttatgacg aagcaggata tatcgcaatc catattcaca gcggacgaag cggtcggaac
    51241 aataaccatc gcagtatccg tgaagtgacg attgtatctg acatcatcca tttagtggaa
    51301 tcagaattgg aaacagatat ccattcagaa ccattttcac tgaattattc aagactggtg
    51361 aatcatttac gactgcttct acaacgaacc catgcgcaac aatatgctgt tttagacacg
    51421 gaaatcgtag aaatggtcaa acgaaaatac ccagaaagct ataagatttc taaaaaaatc
    51481 cgtgtgttgc tgaccaaaga ataccagctc gcgatcacga aagaagaact gggttacttg
    51541 gccatccata tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa caatcatgag
    51601 gaggaaaaaa attgaaagca tatctacaac gaatgggacg ttcccttcaa cttccagttg
    51661 cggtcttacc tgctgctgct ttactagtag ggatcggaaa ctggtgggca tcttatagta
    51721 acgacattgt cgctcatttt ttacaagctg gcggtaacgc agttttaggt caattacctt
    51781 tgctgtttgc cgttgggtta gcactaggga tgtcagtgga caaatcaggt gctgctgcct
    51841 tggcaggttt ggtcgcattt gaagttccag taaatgtttt aaaaactgat tcagtagcta
    51901 ctttattgaa tatcaaacca gaatcagttg atccatcttt tgctcagatc agtaacgtat
    51961 ttattggtat tattgccgga ttgattgcgg cagctcttta taatcgtttc cacgaagcaa
    52021 aattaccgat ggctttatct ttctttagcg gcaagcgtct agtgccaatc ttggctgcct
    52081 ttgtcatgtt gctgatcagt gcagtgctac tgctcgtatg gcctccggtt tactctgctt
    52141 tagtttcttt tgctaaattc attttgagtt taggctgggg tggtgcaggt ctttatggct
    52201 tcttcaaccg tttactgatc cctacaggat tgcatcaagc aatgaactcc gtgttctggt
    52261 ttgacgtagc aggaatcaat gatattggta aatttttggc ttctgaagga acaaaaggcg
    52321 taacaggaat gtatcaagct ggatttttcc caatcatgat gttcgggctt ccagcaggag
    52381 catatgctat ttacagaaat gcacgtccag aaaacaaagc aaaaacagct tccttgatgc
    52441 tagcagcagc ttttgcttca ttctttactg gtgtgacaga accacttgaa ttttcattta
    52501 tgttcgttgc ttggccatta tatgtcttgc atgcaatttt cactgggatt tctttagcag
    52561 tgagtgcatt cttccattgg acagcaggat ttgcctttag tgccggattt gtcgattact
    52621 tcctatcttt gaaaaatccg attgcgaatc agccattgat gttgatcgtc caaggattag
    52681 taacagcagt gatttactac tttggcttta actttgctat caaaaaattc catctgatga
    52741 ctcctggccg cgaggaagca gatctttcag atgacgatac tgcaacgact aattcagaca
    52801 ataaatatgc ggcacaagca agcaaaatct acgctgcatt aggcggagcc gacaatgtaa
    52861 cttcgatcga taactgtact acacgccttc gtttgcaagt caaagataca ggcctgatcg
    52921 atcaaaataa aatcaaagcc actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg
    52981 cgcaagtcat cgtaggtaca gaagtacaat tcgttgcaga tgagatggca aaactacatg
    53041 gtggtgctgc agcacgacct gcacaaacaa acaccgtggt taaaacagaa acatttgccg
    53101 gtgaaacgat cgaacaagat atttacgcta tcgcaaatgg gaaactgatt ccaatcactg
    53161 aagtttcaga tgatgtattc gcggaaaaaa tgatgggtga cggctatgct gtattaccag
    53221 aaaatggaga aatcttctct ccaatcgctg gtacgatcac aaacattttc ccaacgaaac
    53281 atgctgtcgg aatccaaaca gatgctggta tcgaagtctt gcttcatatg ggcatcaaca
    53341 ctgttgactt aaaaggcgaa ccattcactt tatatgtaga agaaggtcaa aaagtcgcaa
    53401 gaggtcaatt gattgcttta gttgatcttg ccgctattca atctgcaggc aaaaacacgg
    53461 atatggtcgt tgtattcaca aatggcgaca aagtccaaag cttagaaatc gaacccgcac
    53521 gtgatgtaaa agcaaatgat aaaatcggct ctgtttccaa caaagcctga gccatcgtct
    53581 tccccagact tttttatcga taaacaaaca ggagctgaga caaaaatctt gtctcagctc
    53641 ctttttccga ataaaaacgg cgttctcctg ccaactccgc ggtaataagt ccagctaaac
    53701 gaaacatatg aagagctatt ttttatttga ctcttcttat tacttgatgc agaacagctt
    53761 tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca
    53821 ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa
    53881 tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat
    53941 cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa
    54001 cgctgcatca cttgcttcaa taatttctaa ctctttacct gtcgcacgct ctaatgcctt
    54061 ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact
    54121 ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt
    54181 tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa
    54241 ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat
    54301 cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc
    54361 aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt
    54421 atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt
    54481 caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc
    54541 ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg
    54601 aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac
    54661 taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca
    54721 tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga
    54781 aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct
    54841 atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg
    54901 aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct
    54961 ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac
    55021 tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa
    55081 atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc
    55141 tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa
    55201 tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat
    55261 aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt
    55321 ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg
    55381 ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg
    55441 aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc
    55501 gctactttca cgacctctat attatcatgg tttattgact accggtatcc agatttctcc
    55561 catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc
    55621 gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa
    55681 ttcttcaggc gattctgcta aagcgcttac aacaagatac gttccttttg ggaatgggat
    55741 gattcgcgta gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt
    55801 cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc
    55861 atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc
    55921 gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg
    55981 tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc
    56041 ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt
    56101 ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc
    56161 ctctccccat tttgtaaaag agatctcggg agcttcgact aacccataat ctgaagaagg
    56221 aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt
    56281 attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg
    56341 attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg
    56401 gtctccatca aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt
    56461 atgcatctct tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt
    56521 gactccttca aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt
    56581 cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg
    56641 tggaaaagat ttttgtatgc cgtatctact gatttctgaa ggcagatatc ctgaccaatc
    56701 acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt
    56761 cactcttgat ccatctagaa gatcgcgatt agccaaagcc aatctacggt ttttgatata
    56821 gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat
    56881 tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta
    56941 atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgatt gcctttttat
    57001 catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga
    57061 aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat
    57121 tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac agattctaag
    57181 taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta
    57241 caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata
    57301 catcatacct cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt
    57361 tgtcagataa ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt
    57421 ttctcacatt ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg
    57481 attatcaaat atgtaaaatc agctactttt ttcttaaacg tttctttata ctaaatataa
    57541 ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac
    57601 gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca
    57661 agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg
    57721 gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa
    57781 cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca
    57841 ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta
    57901 acagagattc ttatagaaca tccgcacttt atcgaacgct tcaaagttaa gatgccattt
    57961 catgcaataa attattcaga taaattaaca actatcatcc ataaatacga tcatattatc
    58021 aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa
    58081 gaagcagttg aatcgcttat tcagcaaaat gcctatcttc ccgcattcga attgatcaat
    58141 gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt
    58201 ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa
    58261 aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca
    58321 aaacgctact ctataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa
    58381 aagaaaaaaa tagataaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat
    58441 agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa
    58501 aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca
    58561 tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctgga
    58621 tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt
    58681 tccgctgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac
    58741 cctaacccta gtgcagcaat cattgttgcg cctaatgcag gtccttcctc catacttaaa
    58801 caatagaccg ttgcatcgaa aatatcagct tggatttgta gccatgcaag atttttcgcg
    58861 cctcctccca ccgaaacaat acgtgtaaat ctgcgacccg ctagttcttt acgtttcaat
    58921 cccaccattg acaaatgctc attaaaaatt tagaatattc acaagtttaa cgacaattat
    58981 gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact
    59041 gcgatcaaag acacgttacc taccgttttc ggttatatcg gtattggact tgcatttggt
    59101 atcgttggga aagctgccgg atttcatcca ctagtcgtca cgttgatgtc cctgctggtc
    59161 tatgctggtt ctgcccaatt tatcacagtc agcatgcttg ctagtcacag cccattgctt
    59221 tccatcgttt tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt
    59281 gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca
    59341 gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt
    59401 cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt
    59461 ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc
    59521 gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa
    59581 cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt
    59641 atcccaagca acttagtgat tgtagttgta acactgattg ggtgcggatt gggggtgtgg
    59701 atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac
    59761 ttggctttct agggtcctac catttgtctt actgaaaaaa ttcgatttac cgcaaccgtt
    59821 gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct
    59881 atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt
    59941 tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggcaggaat
    60001 tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta
    60061 ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct
    60121 tatccgttga acggaccact aacaacgact tttcctaaca tatgtcctgt ttctacgtct
    60181 ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta
    60241 ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt
    60301 tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat
    60361 tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt
    60421 ccaaatggtc ggacaaggtg cttgatttga tcaaagtagc gggtaatatc gaataataca
    60481 gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga
    60541 taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca
    60601 gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc
    60661 ccggatccgt tgatcactaa gatttttttc ccgcgattgg cattttcttc tggagttaga
    60721 ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcagct
    60781 tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac tagttggtat
    60841 tcttgattgc ttcctgctct tgttgttgta cctgcgtaaa aaacgcgatc gccgacagaa
    60901 aatttttgca cttcgtcacc tacagcaaca actttcccga ctccatcaaa acctaagata
    60961 cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat
    61021 actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca
    61081 tcaataaagc tttttggatc atcgatcggc aacccttcat aaaaaccgac taccttcatt
    61141 aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag
    61201 tgttcatttc aatatatata gtatgctatt tgttccacct tttttattca tcataattat
    61261 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
    61321 ttttgtcaca acctcttata aacggtgttc tcctaccaac tcttcggtca caagtcaaaa
    61381 tattcatcaa acatgagaag ctgttttttg aaattgctcc ttgtgactca gagttaaacg
    61441 gcaggctcac aacctctgat ataaacggtg ttcaatcaac tgaccctcgg tattagctca
    61501 aaaaacgaca aaatatgaag agctattttt gtttggctgt tcttattact tgatacagga
    61561 cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt tcactgaaaa
    61621 gcgttggatg ttctttttca aaggtctcta agatctctcg tacttcatca gtagatttcg
    61681 tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata tagattttaa
    61741 agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga tcttgcagat
    61801 ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc gggtcttttt
    61861 caaatgcata aggattcttg aaaatccctc ggccaatcat gatgccatca acgccatatt
    61921 tctcggccaa ttcttctcct gtctggcgat cggggatatc tccattgatc gtgattagtg
    61981 tttgtggagc gatttggtca cgtaaagcaa tgattttagg aatgacttcc caatgtgcat
    62041 ctactttact catctcttta cgcgtacgca aatggacaga aagattggct atatcttgat
    62101 gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg atacgcgtct
    62161 tgacgctgac aggcaaaccg ccagctttag ctgcttcaat caattgtgcc gctacttctg
    62221 gacgaaggat caagccactg ccttttccac gttctgctac attagggact gggcagccca
    62281 tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt tctctaaaaa
    62341 attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct gtaaatgtta
    62401 atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag tttgtaaact
    62461 cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat acaacatctg
    62521 ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc caaaaattag
    62581 tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc tttctcaaaa
    62641 acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta aaagatgttt
    62701 ctgattgagc aaaaaaaaga ctgtgacaaa aatcttatca cagtccattt tccggaataa
    62761 atggtgttca aaagcttgca tttgcggtaa taagtccaac caaacgaaaa ataggaggag
    62821 ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac aacctctcca
    62881 ctaaacggtg ttcaatcagc tgaccctcgg tattcgctca aaaaacaaca aaatatgagg
    62941 aactattttc ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    63001 ttctaacttc tcaatcctct tcctttattg atcaaatacc aagtaacggc gtacaaaaca
    63061 acgatgaata acacaagtac gatcatcgat gttgctactg gcacatcgat cgttccaagg
    63121 aatccgtaac ggaaaccgga gatcatataa acgatcggat tgacttttga aacagcttgc
    63181 caaaagggcg gtaacataga gatggcatag aaaacgccac ctagataagt taacggctgc
    63241 aagacaaatg taggcacgat cgatacatca tcataagact gagcaaaaat cccgttgagc
    63301 agtcctgcta atgaaaacaa gattgccgtc atcaataatg taatgatgac catgaaccat
    63361 gaatacacat gaagtggtac aaagaacaag gaaatgatcg ttacgagaga gccgacgaga
    63421 atactgcgac ccacaccacc aatcacaaag ccccaaataa tcacatgcgt aggaacaggt
    63481 gctaccaaga tttcttcgat atttttctga aatttttgag agaagaagga cgaagaaaca
    63541 ttggcatacg aactagtgat agcagacatc ataatcaaac caggaacgat gaattccata
    63601 tatgaaaaac cacccatgtc accgatccgc ccaccgatca tttttccgaa aataacaaaa
    63661 tacaaggatg tcgtgatcac tggcggaacc agtgtttgga cccatatacg caaatagcgg
    63721 ttcgtttcct tgatagctaa actttttaat gcagtaaaat aaagattaaa catttttctc
    63781 ctccgtatga ttattttctt ctgtgatttt caagaacagt tcttctaaac gattcgactt
    63841 attcctcata gaaagaactt tgattccttg tcttgtaagc tgatcgaaga ttccgttcac
    63901 cccttgattt cgttcaactt cgacagcgat tgtctgctca tcttcaaatg tacaagcata
    63961 tccttcaatc acaggtttgg tatcatatgg cgctaaatca aagataaatg tttcaaattg
    64021 cagttttgac agaagctctt tcatacttgt attctcgatc agttcacccg attggatgat
    64081 tccgatgttg cgacaaagca tttcagcttc ttctagataa tgcgtcgtca aaatgatcgt
    64141 tgtaccattt tcgttcaatt ctcgtagaaa ctcccacatc tcgcgtctca actcgatgtc
    64201 aactcctgct gttggttcat ccaagatcag caaacgtggt tcatgcatca atgccctagc
    64261 aatcatcagt cttcgcttca ttcccccgga aagcatacgt gcacggacat ttctcttttc
    64321 ccaaagattg gactgcttca aatatttttc gctgcgtttc atcgcttctt tgcgagagac
    64381 accgtagtag cctgcttgat tcacgacgat ttgctggacc gtttcaaaag ggttaaaatt
    64441 gaactcttgt ggaaccaatc caatctgttg ctttgctcga actagatcct tgtccagatc
    64501 ataaccgaag acattcactt ttccagatgt tttattgact aaagaagtaa taatcccgat
    64561 cgttgtagac ttcccagctc catttggccc taatagcgca tagaaatctc cttctttcac
    64621 cgaaagatcg attccccgca atgcttcgac cccacttcca tagatctttt tcaaattatt
    64681 tatttcaagc gcatttgcca tttatttcct atctccttat agacatgatt cttaattgta
    64741 tcatggaata cagcagatga aaaaacaact tggcttccac acaaaaacac acctgctaac
    64801 aaagaggaga tgtgttttct agttcatgat catcttcgat agaataacta tctaaacaac
    64861 tctggaactt ttaaaatcat aaaagtaccg acagcaacta ctacgatgac atatagaaga
    64921 tccttataac tgaggttctt gtctttttta ttcatgttaa gctttcctcc gtccttttat
    64981 cgaaagcttt tttcttttat tttaaccgaa ataaataaaa tggaaatagt tattttcaat
    65041 ttctgttttc gctgtttgtt ttacggagaa ctttgccatg gaacgtgcgt ctcgtcgctg
    65101 atgctaagtt ttttcgataa aagctgctag aaggaagaca gcgtggttta ttttgtatcg
    65161 tcttttgcta aatcttccaa gatcatctgg acagcagact cgttcccatt gaattctgcc
    65221 acttttttgg catcaatgta cttcttttga taatcgtctt tttggaccca cgcatcatgc
    65281 ttcgtttgat aaagttaatc aaacggatac cactgacgct gttcatcgaa caacaaactt
    65341 tctttttggg cctgttcaac tatggattta ggataattca aaactttcag caaggcaatc
    65401 gcatttcggg tccttgaagg tccttctttt aatttgaaat caaaactgat tccttttcct
    65461 tctgttactt gttcttcaaa gtggacattc tcacaataat tctttaaaat ctctgtcaat
    65521 tcgatatcat gtgtggcgac aaatgctaaa gatggatact ccgcaagcca ccgaacgact
    65581 gaagcggaag aagcaatccg ttcgatcgta tttgttcctt ttaaaatctc atctacgaag
    65641 cagtagcagc gttctttgga agccacttgg tccagcaaac gtttgatcga tttgatttct
    65701 gaaacaaaat aactgtcgcc ttcaaataaa tcatcttcga ttgccatgga agtcaacaca
    65761 tgtccaggtt gcagggtaaa ctgttctgca atcgctgttt ggatcgtctg tgctagaata
    65821 cagctgatgg ctatactttt gacatacgta gattttcctg atgcattgga acccgtgact
    65881 aatgtattct tctgccagtt gactggatta actaccgctt cttttaacaa tggatgatag
    65941 atatctttcg ctgtcacacc gccctcagta aactctggtc ggcaagtgat cggcatatag
    66001 gtgcgataat tcaaaacggc tgctgcaact tccaattctc ccagcaattc ccacagcttg
    66061 atggcttcag ttgaatgctt ttctaattta gtgagaacga aatgatagga gataaatggc
    66121 agcatgaaca tgatgttgac atattcaaaa atcaattctg cttcactccc gctcttcatt
    66181 cgaaatgaaa aacctaattt agggatgtct cttaatggtt tagtttgctg tttcagctct
    66241 ttttgaagtg gtgtcgtcat ttttgctaac tgattggcgg ctgaaatcgt tgaaaccaaa
    66301 taactcatgc tattcagctc tgtttctagt gtttgtttct tcactagata atagaccgta
    66361 ttgaatacta agccaccgat tgctagatag attcctagta cttgccctaa aagaagcaaa
    66421 cctacaccca ccagcggaag caagccaaga aaaataaata aacctacgcg tccaatcttt
    66481 ttactttttc catccgctag atattgtttt gcaaaattat gatctttttt tcctaaacga
    66541 gcgaattgat attggattct ttctctcagt tgaggatttt cctgataaaa agcaattagt
    66601 ttttctaact gataatcttc tccaaaatca aagctacgta aacgctgata caacgcttct
    66661 gaacctacac tagaataggt actgttgatc ccttcaaata cttcgaacat atccaagtca
    66721 taccaagtaa gatcgtcgat ttcactatcc catgaacgga attttttctc cgtgagccac
    66781 gcatccttca aactttcttc tttatcaaat ctcggttgat aaggaatttt tccccaatta
    66841 ctacgaactg tttcctttac tttcaaacgt gaaaaaatgt ctaaaacaac taataaaata
    66901 acaactgcaa taataattaa aacgataaat tctctttggc ccatatccct ctccgttcct
    66961 tgatttgtta gttttagtat agcactgttg cgctatttga aaagggggta caaaaataac
    67021 tgtcttcgtt acctaattcg tatcaatcac accaaaataa aaacaccttc caaaaaagaa
    67081 gatgtcttta aaacgagata caattttttg tcattcactt caggtaaatt agagccaatt
    67141 aaaccctccg caacttgacg gagatggaga agatgccccc gtctaatcta gcggtcatct
    67201 ctcctcccat tccttccacc aatttttttg acaggtaaag cccgattccg ctagattccg
    67261 tatttgtgcg gctggtattt tcggtataaa aacgctggaa gactttttca acagaggttt
    67321 gcgctcctgg tttgatagga ttggccactg ttaatcgaat gaatgaatcc tcttttgaaa
    67381 atgtcacctt tgcttgtcca tctgcataac gcaataaatt ctgtatgata ttttgcagca
    67441 ctcgcagcaa catgagcgga tctgctaata cagttgtatt tatcgagtgt tcagcgaacg
    67501 ctacctcgat atttttctca gaaaattgtt cataaaaaga gaaaaatact tcttccacct
    67561 ctttagaaaa agaaagcgta tggagatcca tttctttttg cttcgtctct attcgagcaa
    67621 gatcataaaa ctgatccgtc atactgagta atcttgcaac agattgtttg atgatcgtta
    67681 aatacttctc ccgtttctcc gtatcctcta cctgttccag taattgtaca taaccgttaa
    67741 tagcagtcaa aggagttctg agatcatgag aaaggccgac gatcgatagt ctgatgtttt
    67801 cttccatttc ttgaaatgct tgtccttttt gttcattttc atccacccat gtattcagtg
    67861 catctactag gtccattaga ttcttttctc gaaattcgac tgaaagacga ccaccatatt
    67921 cttgtcgttc agaaagagtg gcgattttct tagttaatct cttgatttcc ctttgcagga
    67981 agaaaataaa acttcccata aagataacaa cgagaagcaa cagccactta atcaaatcta
    68041 aaccccactc cccacaccgt atgaatatac acagcatgtg cctttgctag tttccctcga
    68101 agattggaaa tatgcatatt cactgttttc tcactatcaa aatatggttc ttgccaaact
    68161 gtttcataaa gatttgctcg cgaaaatact tttttcggat gttctaaaaa aagcttcagc
    68221 agatcgaatt ctcttcctgt caaatggaca gccacaccat tcacatttac atccctcgac
    68281 tctttgtcca gtaagatttc cttgtaatag atgcgttgcg acagatcttt tacttggaca
    68341 gaagtctgat gacgaagttg gatgccgatt cgtaacaaca gttcttcgat atcaaatggt
    68401 ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt
    68461 tttgctgaaa gaacgatgat cggaacattc gatgtctgtc ggatttctgc taacaatgct
    68521 tcacctgaaa gccctggaag catgagatct agaaggatca aatcgacagg atatgtgctt
    68581 aacaataatt ttccttccgt acctgaataa gcatcaagaa gtttgtatct ctgttcaaga
    68641 acctcctcca gtaaagaatg aacagcttca tcgtcttcga taatcaaaat agtttccagc
    68701 aggaacacct acttaatgtc agatttttgg aaaataaaca ttcctagagc tgatgatacc
    68761 acgattgtta ctagactgac cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc
    68821 gcaaagtccg tatacatgag ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct
    68881 ttgatttttg cgaaaacacc gggaacgaag gccgttccca tgatataaag aaaagaaaga
    68941 agcaaactga cactggtact tctagtgatg aatgtcaaag ccactggaac agataatgtc
    69001 gctaatatca gcagaagcaa tacaggaaat acttttagaa cataatttcc atagtttttg
    69061 attccctcaa cttcaccaaa tccgaaagca agtgtgtaag caatcatggc tagccccgta
    69121 atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga cacataataa
    69181 gtcattcgat tccgtcctaa agataacgta ttgcgaatcg taccgttttg aaattcttcc
    69241 ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc cattttttcc
    69301 accattaaaa tccctgttgc accgttgatg actgtatccg ttgcttggtt taattcattg
    69361 ccgtattttg tatgcatata ccattcgttc caagaataaa aaagcggaag tacgaccatc
    69421 agtcctagtg agacccacat tttacggctc ttccatactt ttattttttc agcagttaat
    69481 agattaaaca tgtggattgc ctccagttaa ttgtaagaaa taatcttcca gtttttgccc
    69541 aactaggtgg ataccatcca caggaacttt tgcctctacc aatgcttgat tgatggctgc
    69601 tacttggtca attccttcaa aaagctggat aacacggcta gattggacaa aataatccct
    69661 ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt caatttttat
    69721 aaattgacga gattcttgtg ccaattcttc tttcgataat tctttgatca agtgtccttc
    69781 atgcaaaata ccgtagtgag tggcgatctg tgacaattca tctagcaagt ggctagaaag
    69841 caagacggtg atgccccgtt cttttaccaa acgttggatg atttctcgca tttctgccat
    69901 ccctgctgga tccatcccat tcgtcggttc atccaaaatc aaaaattctg gattcgtgat
    69961 cagtgcattt gcaatagcta aacgctggcg catacctaaa gagaaattct ttgctttctt
    70021 tttccctgta ttttccaatc gcatcatttt cagcaggtct tcgatttctc tatccgacac
    70081 accgccgttc gctgcttgga tacgtaggtt atctcttgca gttaattctg gataaagagc
    70141 aggggtttca atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa
    70201 taattcgatc gaaccttcat caatcgagat caaacccata atagttctca taaaggtaga
    70261 cttacccgct ccattctccc caatcaaacc atagatcatt ccacgtttga tttcgatcga
    70321 tactttatct aatgctttgg cagcaccgta ttttttggtt ataccagttg ctttcaagac
    70381 cgtttcattt ccaaaatcca tacttatcct tctttcttat tgataagtga agtatagttg
    70441 acgggtataa agaaagcagc cagaaatgta taaagaaagt gtaaattttc caattgaatt
    70501 tcaagaaaaa tcagactgtc tcgcagacgt ggttgagttg tctgccaatc aaagagcgca
    70561 ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat cgttgacatc
    70621 agacgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata aaaagcaaaa
    70681 gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt tgggatttac
    70741 aattagctac caaataagag gttatgacaa aagacttttc acaatccctt cttccgaata
    70801 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa acatgagaag
    70861 caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcagaa
    70921 gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt atagatcgaa
    70981 tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa aaaggcactt
    71041 tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc agcattgctt
    71101 cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta acgttttgag
    71161 aactgtgaag ctttacgggc tttcttaaga cctggttaaa caaaactcta ttttattgca
    71221 ttttacagga atgtatttca tgcgaaatta ataagtcttt gtttatagag ttttactagg
    71281 gtataaaaag ttgtatgtta tcattacctc aaaacattac ctcagcttgg cgagttggtt
    71341 gattgaagaa taaatcattt atctagatat ctcaataaaa aatgatttct aagaaacttg
    71401 tattttcgtg ttattatttt tttgccctat caaaatttct aattcttcta gcgtaatatg
    71461 attatgacac ataatcgatt caattacttc aatacctatt tttttcgaga aagatccctt
    71521 ttgttcactc acaaaaatac tttcagaaga catatataag gaacgaagtt ttggaattag
    71581 tatatcgtac aaatagacca ccataaaatc gtccttgcaa ccattctcaa aaagaattaa
    71641 atttgctgat tgtgttacat atatcactga attacattca tggtcaattc caaaaacaat
    71701 gtctacttca ttttcttttc taattcgcgc agttacattt ttcaaacttt caccattact
    71761 tataaatgct ttagttactg taagctctga tgtttgttca gatattcctt ttataatatt
    71821 ttgttcatca gccacattcc ccataaatcc gccgattcct tttaagtaat aattctcacc
    71881 taagagtcgc ttgcattcac tgaattgata aaaaaaccag ttaaaaaaat cttctggaaa
    71941 aataaattct gatcgaactt tatttatatt tttagctcct attaatttta taactcgatt
    72001 aatgttcact tgctgcgatg aacatataac tgaataaatt gagttgttta attcaaaaaa
    72061 acaactttaa catatgcatc attaattcgt tattttttag gtagatattg atcattgtta
    72121 tctctctttt tttgcttccc atactccaac ttagaaattg ccgttataaa atttatttta
    72181 aaggtgactt tttcccctga agtatcggta gtaggaatac ttaatacctc agcttctgag
    72241 gaggtatccc aattatagtt atactttgca tcatcaccat aaagaacaat tccatttttt
    72301 ttatcttgtg ccgttagctt tactggttcg cttacttttc ttgttaatat cttggacttt
    72361 acatcatcga tattgcttat tttatcaagc ttaaataatt tacaaattaa tttcgtacta
    72421 gtttttatat ttga
//
LOCUS       DACDLI010000005        68274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDLI010000005 DACDLI010000000
VERSION     DACDLI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.005"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            157..675
                     /locus_tag="IV353_000340"
     CDS             157..675
                     /locus_tag="IV353_000340"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9008620.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            803..1486
                     /locus_tag="IV353_000341"
     CDS             803..1486
                     /locus_tag="IV353_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9008621.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1795..1868
                     /locus_tag="IV353_000342"
     tRNA            1795..1868
                     /locus_tag="IV353_000342"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1829..1831,aa:Arg,seq:tct)
     gene            2027..2386
                     /locus_tag="IV353_000343"
     CDS             2027..2386
                     /locus_tag="IV353_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9008622.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2509..4140
                     /locus_tag="IV353_000344"
     CDS             2509..4140
                     /locus_tag="IV353_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9008623.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDGFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4202..4843)
                     /locus_tag="IV353_000345"
     CDS             complement(4202..4843)
                     /locus_tag="IV353_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9008624.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5125..5433
                     /gene="rplU"
                     /locus_tag="IV353_000346"
     CDS             5125..5433
                     /gene="rplU"
                     /locus_tag="IV353_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9008625.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5460..5804
                     /locus_tag="IV353_000347"
     CDS             5460..5804
                     /locus_tag="IV353_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9008626.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5817..6110
                     /gene="rpmA"
                     /locus_tag="IV353_000348"
     CDS             5817..6110
                     /gene="rpmA"
                     /locus_tag="IV353_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9008627.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6517..9351
                     /locus_tag="IV353_000349"
     CDS             6517..9351
                     /locus_tag="IV353_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9008628.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9542..10033
                     /locus_tag="IV353_000350"
     CDS             9542..10033
                     /locus_tag="IV353_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9008629.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10136..11125
                     /gene="manX"
                     /locus_tag="IV353_000351"
     CDS             10136..11125
                     /gene="manX"
                     /locus_tag="IV353_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9008630.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11158..11961
                     /locus_tag="IV353_000352"
     CDS             11158..11961
                     /locus_tag="IV353_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9008631.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11982..12896
                     /locus_tag="IV353_000353"
     CDS             11982..12896
                     /locus_tag="IV353_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9008632.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12979..13353
                     /locus_tag="IV353_000354"
     CDS             12979..13353
                     /locus_tag="IV353_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9008633.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13492..13737
                     /locus_tag="IV353_000355"
     CDS             13492..13737
                     /locus_tag="IV353_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9008634.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13909..14973
                     /locus_tag="IV353_000356"
     CDS             13909..14973
                     /locus_tag="IV353_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9008635.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15219..15644
                     /locus_tag="IV353_000357"
     CDS             15219..15644
                     /locus_tag="IV353_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9008636.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15637..16086
                     /gene="nusB"
                     /locus_tag="IV353_000358"
     CDS             15637..16086
                     /gene="nusB"
                     /locus_tag="IV353_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9008637.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16183..17037
                     /locus_tag="IV353_000359"
     CDS             16183..17037
                     /locus_tag="IV353_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9008638.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17038..18375
                     /locus_tag="IV353_000360"
     CDS             17038..18375
                     /locus_tag="IV353_000360"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9008639.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18380..18604
                     /locus_tag="IV353_000361"
     CDS             18380..18604
                     /locus_tag="IV353_000361"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9008640.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18606..19496
                     /locus_tag="IV353_000362"
     CDS             18606..19496
                     /locus_tag="IV353_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9008641.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19505..20323
                     /locus_tag="IV353_000363"
     CDS             19505..20323
                     /locus_tag="IV353_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9008642.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20430..20879
                     /locus_tag="IV353_000364"
     CDS             20430..20879
                     /locus_tag="IV353_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9008643.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20897..22579
                     /gene="recN"
                     /locus_tag="IV353_000365"
     CDS             20897..22579
                     /gene="recN"
                     /locus_tag="IV353_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9008644.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22634..22750)
                     /locus_tag="IV353_000366"
     CDS             complement(22634..22750)
                     /locus_tag="IV353_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9008645.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22823..23779)
                     /locus_tag="IV353_000367"
     CDS             complement(22823..23779)
                     /locus_tag="IV353_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9008646.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23950..24318
                     /locus_tag="IV353_000368"
     CDS             23950..24318
                     /locus_tag="IV353_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9008647.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24488..24919
                     /gene="mraZ"
                     /locus_tag="IV353_000369"
     CDS             24488..24919
                     /gene="mraZ"
                     /locus_tag="IV353_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9008648.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24940..25899
                     /gene="rsmH"
                     /locus_tag="IV353_000370"
     CDS             24940..25899
                     /gene="rsmH"
                     /locus_tag="IV353_000370"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9008649.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25904..26305
                     /gene="ftsL"
                     /locus_tag="IV353_000371"
     CDS             25904..26305
                     /gene="ftsL"
                     /locus_tag="IV353_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9008650.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26306..28498
                     /locus_tag="IV353_000372"
     CDS             26306..28498
                     /locus_tag="IV353_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9008651.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28524..29486
                     /locus_tag="IV353_000373"
     CDS             28524..29486
                     /locus_tag="IV353_000373"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9008652.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29500..30861
                     /locus_tag="IV353_000374"
     CDS             29500..30861
                     /locus_tag="IV353_000374"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9008653.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30887..31975
                     /gene="murG"
                     /locus_tag="IV353_000375"
     CDS             30887..31975
                     /gene="murG"
                     /locus_tag="IV353_000375"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9008654.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31990..33210
                     /locus_tag="IV353_000376"
     CDS             31990..33210
                     /locus_tag="IV353_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9008655.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33366..34691
                     /gene="ftsA"
                     /locus_tag="IV353_000377"
     CDS             33366..34691
                     /gene="ftsA"
                     /locus_tag="IV353_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9008656.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34714..35955
                     /gene="ftsZ"
                     /locus_tag="IV353_000378"
     CDS             34714..35955
                     /gene="ftsZ"
                     /locus_tag="IV353_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9008657.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35972..36649
                     /locus_tag="IV353_000379"
     CDS             35972..36649
                     /locus_tag="IV353_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9008658.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36663..37262
                     /locus_tag="IV353_000380"
     CDS             36663..37262
                     /locus_tag="IV353_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9008659.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37277..37564
                     /locus_tag="IV353_000381"
     CDS             37277..37564
                     /locus_tag="IV353_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9008660.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37613..38395
                     /locus_tag="IV353_000382"
     CDS             37613..38395
                     /locus_tag="IV353_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9008661.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38416..39123
                     /locus_tag="IV353_000383"
     CDS             38416..39123
                     /locus_tag="IV353_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9008662.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39385..42183
                     /gene="ileS"
                     /locus_tag="IV353_000384"
     CDS             39385..42183
                     /gene="ileS"
                     /locus_tag="IV353_000384"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9008663.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42232..43758)
                     /locus_tag="IV353_000385"
     CDS             complement(42232..43758)
                     /locus_tag="IV353_000385"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9008664.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43773..44420)
                     /locus_tag="IV353_000386"
     CDS             complement(43773..44420)
                     /locus_tag="IV353_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9008665.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44604..44933)
                     /locus_tag="IV353_000387"
     CDS             complement(44604..44933)
                     /locus_tag="IV353_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9008666.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45110..45838
                     /gene="sfsA"
                     /locus_tag="IV353_000388"
     CDS             45110..45838
                     /gene="sfsA"
                     /locus_tag="IV353_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9008667.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45854..46867
                     /locus_tag="IV353_000389"
     CDS             45854..46867
                     /locus_tag="IV353_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9008668.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46867..48144
                     /locus_tag="IV353_000390"
     CDS             46867..48144
                     /locus_tag="IV353_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9008669.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48207..50909
                     /locus_tag="IV353_000391"
     CDS             48207..50909
                     /locus_tag="IV353_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9008670.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51061..51378
                     /locus_tag="IV353_000392"
     CDS             51061..51378
                     /locus_tag="IV353_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9008671.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51408..51728
                     /locus_tag="IV353_000393"
     CDS             51408..51728
                     /locus_tag="IV353_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9008672.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51836..53296
                     /locus_tag="IV353_000394"
     CDS             51836..53296
                     /locus_tag="IV353_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9008673.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53364..53585
                     /locus_tag="IV353_000395"
     CDS             53364..53585
                     /locus_tag="IV353_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008674.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53616..53798
                     /locus_tag="IV353_000396"
     CDS             53616..53798
                     /locus_tag="IV353_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9008675.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53798..54211
                     /locus_tag="IV353_000397"
     CDS             53798..54211
                     /locus_tag="IV353_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9008676.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54334..55515
                     /locus_tag="IV353_000398"
     CDS             54334..55515
                     /locus_tag="IV353_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9008677.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55904..55977
                     /locus_tag="IV353_000399"
     tRNA            55904..55977
                     /locus_tag="IV353_000399"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55938..55940,aa:Arg,seq:cct)
     gene            complement(56046..57185)
                     /locus_tag="IV353_000400"
     CDS             complement(56046..57185)
                     /locus_tag="IV353_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9008678.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEVID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(57301..57936)
                     /locus_tag="IV353_000401"
     CDS             complement(57301..57936)
                     /locus_tag="IV353_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9008679.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(58019..58441)
                     /locus_tag="IV353_000402"
     CDS             complement(58019..58441)
                     /locus_tag="IV353_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9008680.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(58459..58779)
                     /locus_tag="IV353_000403"
     CDS             complement(58459..58779)
                     /locus_tag="IV353_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9008681.1"
                     /translation="MNPYEKIKELTKQNGISVRELEKRLGYSNGYFSKWKSVSPNSEG
                     LAKVSDYFGVSIDYLLGRDKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            59080..59856
                     /locus_tag="IV353_000404"
     CDS             59080..59856
                     /locus_tag="IV353_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9008682.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            59871..60074
                     /locus_tag="IV353_000405"
     CDS             59871..60074
                     /locus_tag="IV353_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9008683.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            60090..60428
                     /locus_tag="IV353_000406"
     CDS             60090..60428
                     /locus_tag="IV353_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9008684.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(60683..61348)
                     /locus_tag="IV353_000407"
     CDS             complement(60683..61348)
                     /locus_tag="IV353_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008685.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDPLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            61403..62101
                     /locus_tag="IV353_000408"
     CDS             61403..62101
                     /locus_tag="IV353_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9008686.1"
                     /translation="MQELVILKNKEAVTTSIQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNTKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            62279..62621
                     /locus_tag="IV353_000409"
                     /pseudo
     CDS             62279..62621
                     /locus_tag="IV353_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            62614..63285
                     /locus_tag="IV353_000410"
     CDS             62614..63285
                     /locus_tag="IV353_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9008687.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            63291..63977
                     /locus_tag="IV353_000411"
     CDS             63291..63977
                     /locus_tag="IV353_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008688.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            63984..64835
                     /locus_tag="IV353_000412"
     CDS             63984..64835
                     /locus_tag="IV353_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9008689.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            64847..65116
                     /locus_tag="IV353_000413"
     CDS             64847..65116
                     /locus_tag="IV353_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008690.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            65278..65598
                     /locus_tag="IV353_000414"
     CDS             65278..65598
                     /locus_tag="IV353_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008691.1"
                     /translation="MDKKAAWRKLMLLIQDENWQEDEAVVAEVQRLEKIANGRIRKKP
                     DKRKLRKGKIIVVLYEGNILMQGTARELSAETEYTSGTIRTYAWRNHVDKKGHEYKYL
                     EGSK"
     gene            65595..65984
                     /locus_tag="IV353_000415"
     CDS             65595..65984
                     /locus_tag="IV353_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008692.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEEQR"
     gene            65981..66574
                     /locus_tag="IV353_000416"
     CDS             65981..66574
                     /locus_tag="IV353_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9008693.1"
                     /translation="MNKQETIKKLESIKAIGNDAIAACYNESMNAGITLMKKIDEPQK
                     HVMPKFFDKWAKQVLEKRDKFYAISLIARAGWGYGVDFELSENGLSSENKELLYWLVD
                     KCSDNYPNKKKAIEALLYGYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAA
                     TERYGAKWKNTFTESEIKAIDERYWPFAVPVEEEAEG"
     gene            66571..66948
                     /locus_tag="IV353_000417"
     CDS             66571..66948
                     /locus_tag="IV353_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008694.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQQIVFEWLKLTAPTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKEQFEVLAAFAQWGLEQEEAE"
     gene            66945..67148
                     /locus_tag="IV353_000418"
     CDS             66945..67148
                     /locus_tag="IV353_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP9008695.1"
                     /translation="MNNRHRRITKLRKQELNVLKAKFEKEYGVSAEEAYKVASQCVAD
                     ASNAIRKFGISILSDDRKWEDIE"
     gene            67145..67354
                     /locus_tag="IV353_000419"
     CDS             67145..67354
                     /locus_tag="IV353_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008696.1"
                     /translation="MKVYVVRFGNKFYRSDERSIGNNVLFLVDFLQNARWFDNLEEAN
                     QVSRRLGGLTQVYELVTIDHEGVER"
     gene            67351..67644
                     /locus_tag="IV353_000420"
     CDS             67351..67644
                     /locus_tag="IV353_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008697.1"
                     /translation="MKLKDGFYASSHGIGGLMLGMPTKNPKTRKKPKFKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            67674..68114
                     /locus_tag="IV353_000421"
     CDS             67674..68114
                     /locus_tag="IV353_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9008698.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            68189..>68274
                     /locus_tag="IV353_000422"
     CDS             68189..>68274
                     /locus_tag="IV353_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP9008699.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAV"
BASE COUNT        22919 a        11366 c        14442 g        19547 t
ORIGIN      
        1 gtcaattatt tataataaaa aatgagataa agatgaggaa atagaattag aaacatataa
       61 ttattaaagg gaaacatttt tacagctata atttgaaaaa aatttatcgt tattatatga
      121 ttaagaagga ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact
      181 tttataagtt ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt
      241 ccccgaattt ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat
      301 gcacaaggaa atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat
      361 attattacat attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt
      421 gaaaacaacc acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga
      481 gtagtaaaaa agagaaatat tatcggtagt cccatctttc agattccata tattggacta
      541 gtcctttcaa aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc
      601 ctaacaggaa tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca
      661 cagaaatttt tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc
      721 tgaacatgaa acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata
      781 atgtgtgaag aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga
      841 agaactattg gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact
      901 ggaagaagaa gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt
      961 gaaattttta cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg
     1021 tttttcatca agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga
     1081 tcgtttcgat gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga
     1141 taaagtgact ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta
     1201 tgactttatt tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt
     1261 acgtttgtta agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat
     1321 cttgctccca gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga
     1381 atttcttcgt gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga
     1441 tggtgtgatc ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc
     1501 agcctgtaga taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat
     1561 atttgtaagt caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa
     1621 tgtagctcct gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa
     1681 aagattgatt tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc
     1741 gtattgacag aagcaattta attatctata attattgatg ttccgctgat tcttgtcata
     1801 gtagctcagc tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc
     1861 ctgtgacgct aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt
     1921 gccaatattg tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat
     1981 tataaggagc aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc
     2041 tagaatttta gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt
     2101 gagaactgat tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag
     2161 aaaatctgtt caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg
     2221 actactttat ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat
     2281 atctattttt tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa
     2341 acaaggtggg ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata
     2401 agaatttatc taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat
     2461 tctaatgaat ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat
     2521 tatgtttcct cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat
     2581 cttttggctg ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta
     2641 tcaagcggag ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt
     2701 ggcggtttat caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc
     2761 ttgatgtcta ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg
     2821 ggtctatttt tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca
     2881 gcacccgtaa ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc
     2941 gataatttat ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat
     3001 caaacgatag gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta
     3061 gggatgttta tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc
     3121 attttggcaa cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt
     3181 ccacaaacat tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg
     3241 caacaagtgg cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta
     3301 tgtggtgctt cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg
     3361 gcgcaaggaa ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc
     3421 atcgcccgta caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata
     3481 catgcagttg tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca
     3541 atgccagcac ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg
     3601 atcaaggagc tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg
     3661 atttgtacag ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt
     3721 gtattttttg ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa
     3781 agaatggggc tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt
     3841 ccactgtttt ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac
     3901 gatggtgtca ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt
     3961 tttgatggat ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg
     4021 cagccagaag tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat
     4081 caaacagttg ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa
     4141 aacaatgaaa aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt
     4201 tttaatattg ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa
     4261 gggagcgatt gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt
     4321 ctgaccattc gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt
     4381 gaccatgatt tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc
     4441 caagtgtcgc aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat
     4501 gcgcaaatac ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg
     4561 tccgtttatt gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat
     4621 acacctcttc tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt
     4681 gaccgctaaa aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca
     4741 tcagtttagg atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca
     4801 ttcttaaaga agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt
     4861 cttttattat aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg
     4921 atagaaaagc agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg
     4981 actaacggtt gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa
     5041 ccgctcagaa caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta
     5101 tatcagaagg aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta
     5161 aaagttgaag tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa
     5221 gttgtttttg acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact
     5281 gttgcaggtg caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt
     5341 actttcaaat acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat
     5401 acaaaagtgg ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata
     5461 tgatcaaagg aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg
     5521 ggcatgctga tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag
     5581 cgatcagcac tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga
     5641 atgaagaaga aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa
     5701 acaatattgc tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg
     5761 aaaactcaga atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt
     5821 tgttatctat gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg
     5881 gccgtgactc agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag
     5941 gtggatcaat tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta
     6001 tcggtggaga cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag
     6061 gccgtaacaa aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta
     6121 tgattaaaga ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag
     6181 ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt
     6241 tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta
     6301 tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt
     6361 atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa
     6421 acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc
     6481 ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt
     6541 tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta
     6601 acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg
     6661 aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata
     6721 tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa
     6781 acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa
     6841 atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg
     6901 tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat
     6961 tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa
     7021 ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta
     7081 tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat
     7141 caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa
     7201 gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa
     7261 tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta
     7321 ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt
     7381 cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg
     7441 cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat
     7501 ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat
     7561 catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc
     7621 ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag
     7681 ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag
     7741 ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt
     7801 ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct
     7861 ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat
     7921 ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat
     7981 gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa
     8041 atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt
     8101 ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg
     8161 gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat
     8221 caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt
     8281 atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct
     8341 ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat
     8401 gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa
     8461 attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt
     8521 ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca
     8581 ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa
     8641 cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa
     8701 agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt
     8761 ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag
     8821 atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa
     8881 cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg
     8941 gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt
     9001 atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt
     9061 tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac
     9121 ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg
     9181 gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca
     9241 gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg
     9301 ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa
     9361 aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa
     9421 ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg
     9481 gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca
     9541 gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt
     9601 ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga
     9661 taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat
     9721 aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat
     9781 gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa
     9841 gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt
     9901 ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt
     9961 agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat
    10021 caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta
    10081 gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt
    10141 agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat
    10201 gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga
    10261 tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt
    10321 attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga
    10381 gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta
    10441 cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc
    10501 aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc
    10561 agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa
    10621 aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc
    10681 ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga
    10741 tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat
    10801 tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt
    10861 gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa
    10921 agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt
    10981 gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt
    11041 cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc
    11101 aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg
    11161 tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt
    11221 cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc
    11281 ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg
    11341 gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt
    11401 attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt
    11461 cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc
    11521 gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac
    11581 atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt
    11641 atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt
    11701 atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg
    11761 gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa
    11821 ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt
    11881 tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc
    11941 gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca
    12001 aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat
    12061 ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa
    12121 aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta
    12181 atacacaccc atacattgct tctccaattt taggggttac attggctctg gaagaagaac
    12241 gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg
    12301 gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg
    12361 cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag
    12421 gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg
    12481 gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt
    12541 caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct
    12601 taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc
    12661 cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt
    12721 ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg
    12781 cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta
    12841 tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca
    12901 taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg ttcataaaca
    12961 aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt ggtaattgat
    13021 gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtga taatggtttt
    13081 gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga agaagtggat
    13141 tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc cattcaaacg
    13201 aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc
    13261 agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga tgtagtgaaa
    13321 cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc taagaggagg
    13381 ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca attatttgtc
    13441 ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat tatgagttgg
    13501 ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat caccagtaaa
    13561 ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc aagtattgga
    13621 caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat tccatctatt
    13681 ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg atcataagtt
    13741 tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt ttggctttat
    13801 ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa
    13861 agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt
    13921 gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt
    13981 ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact
    14041 ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct
    14101 caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta
    14161 cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat
    14221 tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa
    14281 ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg
    14341 gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc
    14401 gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg
    14461 atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc
    14521 gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat
    14581 atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata
    14641 gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa
    14701 ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat
    14761 tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca
    14821 gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga
    14881 atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg
    14941 actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa
    15001 aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa
    15061 gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg
    15121 cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg
    15181 gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga
    15241 ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa
    15301 ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca
    15361 gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg
    15421 aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta
    15481 aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg
    15541 atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa
    15601 caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac
    15661 gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca
    15721 aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat
    15781 gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa
    15841 gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc
    15901 tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat
    15961 gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac
    16021 cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agaagctggg
    16081 gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc gggaaaattc
    16141 tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga attgattaac
    16201 ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca aaaattgaaa
    16261 gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc agccagccaa
    16321 atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc attggtagag
    16381 cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa
    16441 gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat agatgaagaa
    16501 aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc gatgaattta
    16561 ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg catcatgaaa
    16621 atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat cggacgaagc
    16681 aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac tgttactgtt
    16741 gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat tttagtcgta
    16801 gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata
    16861 gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa
    16921 gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc aatgactatc
    16981 acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg tgaatagatg
    17041 gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa atttgatgcc
    17101 gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg tttacgtgcc
    17161 aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc tattatgttc
    17221 aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt
    17281 gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga gcacatggag
    17341 cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag
    17401 gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc
    17461 cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa aagaagatat
    17521 ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc agcggacaat
    17581 gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat gatcatcggt
    17641 cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg
    17701 atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga taccacgatc
    17761 gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga attagctgtt
    17821 cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt gcagcaggcg
    17881 tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg ttcctatgta
    17941 tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca gttgaaacag
    18001 cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac gatcgcatta
    18061 agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg ccaagaaact
    18121 gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca acagcagcag
    18181 cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat catgggaaga
    18241 ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa
    18301 ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt acaagagata
    18361 gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc aagaattaga
    18421 aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc tagattcgtt
    18481 taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag cagaagagac
    18541 cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa acgaggaaag
    18601 ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa aaagaaatct
    18661 tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg agttattcag
    18721 tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt gcttcctttg
    18781 atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg gtgcatacgt
    18841 attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt agaggaaaac
    18901 ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat ggtttgctta
    18961 caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag cttctgctgc
    19021 ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa gctgcagata
    19081 tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat gaaagaaaga
    19141 cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag caacctgaag
    19201 aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt cagataagag
    19261 atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct ggtaaagatg
    19321 aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt
    19381 tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag gacgtttttt
    19441 cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag aaatgaggga
    19501 aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat ttgaaacaag
    19561 agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa acgagcggtt
    19621 tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag gtaaaaaact
    19681 tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat ttgatctatc
    19741 tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta ctgatgcagc
    19801 tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc agctagcttg
    19861 gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa
    19921 acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt cttttatttc
    19981 actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag aagtgattgc
    20041 tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg gaattgttcg
    20101 tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg cacaaggtgg
    20161 atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa atatcgagtt
    20221 cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta ttaatatggc
    20281 agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat
    20341 caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta tcattgatta
    20401 agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta
    20461 ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa atcctgaaaa
    20521 gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa ctgaaactga
    20581 tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa acaaacgaag
    20641 atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt gaccaaatgg
    20701 aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca aatttgattg
    20761 ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat gttttgatga
    20821 ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat tacctttaag
    20881 ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga ttattccgga
    20941 attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa
    21001 atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa gcgattatat
    21061 acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa aacaagaagg
    21121 attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc tgattgttcg
    21181 acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt
    21241 agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa acgaacatca
    21301 ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag atgtagcttt
    21361 ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc tggaaaaaag
    21421 ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata tgctccattt
    21481 ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga aattaaaaga
    21541 agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg caggttacgg
    21601 agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg tttcagaact
    21661 gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata tccaaagtgc
    21721 ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt tattggagtt
    21781 agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac agttgaaacg
    21841 taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca ccgaagagct
    21901 agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa
    21961 agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa
    22021 agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa acactgagtt
    22081 tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat ttgaccaaat
    22141 cgaattttac attacgacca atccaggaga gccactgaag cctttggtga aagtcgcttc
    22201 tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt ctgagcaagg
    22261 tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag ttgcacaggc
    22321 catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca tcactcatct
    22381 gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag tgattgatgg
    22441 acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag aaattgcacg
    22501 tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag aactattgaa
    22561 actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag cagctgatag
    22621 ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat ccaatcgtta
    22681 aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt gtttgttttt
    22741 tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac aatctttttt
    22801 ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa taggtagtta
    22861 cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc ctttcagcaa
    22921 taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata gttaaaataa
    22981 tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg attgctgaaa
    23041 aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc attgcctgct
    23101 ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct tggaaaagtt
    23161 tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct ctgaaataaa
    23221 cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg gatacttgaa
    23281 gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg ttgtaagaga
    23341 gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata tcactttttg
    23401 gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta attgtgataa
    23461 gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga aaataaccgc
    23521 taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt gtcaactttg
    23581 attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat
    23641 ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta ggacgttcta
    23701 cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc tcattatcta
    23761 atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt ataacggata
    23821 gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca taaatttaaa
    23881 taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat aaaaaaggag
    23941 tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta cccggtaatc
    24001 gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata
    24061 aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt gtctagaaat
    24121 acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat gggaatttgt
    24181 attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta tttcaagatg
    24241 acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac
    24301 atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt
    24361 gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg atttcccaga
    24421 aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga ggaatggggg
    24481 gctttcaatg ttgatgggtg aattccaaca taatattgat gcaaaaggac gtttaatcgt
    24541 gccctcaaaa cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg gattggatgg
    24601 ctgtcttttc ggctatccga tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat
    24661 gccgttggct aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg ctgccactga
    24721 atgcgaactt gataaacaag gacgaatcaa tatacccagc acgttaagaa attatgcagc
    24781 tttgacaaaa ggttgcgtag tcatcggtgt atctaaccga attgaaattt gggatgagac
    24841 acgatggcaa gatttttctg cagcagcaga agagaacttt gacgaaattg ctgaaagtat
    24901 gattgatttc ggactataag aataggaaga ggctgactaa tgactgaaac atttcaacac
    24961 tatacagtca tgttgaaaga aacggtagat ggattgaaca tcaaaccgga tggcgtttat
    25021 gtagactgta cactcggcgg tgccggacat agtgaatatt tattaagcca actaagttct
    25081 aaagggcatc tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt
    25141 ttagcttctt atgtcgaaca aggtatggtc acatttatca aagcgaactt cagagaatta
    25201 gaagaacgct tgagtgagca tgtagataag gtagacggca ttttgtatga tctaggggtt
    25261 tcctctcctc agctagatga agcagaacgt ggatttagtt atcatcaaga tgcaccgttg
    25321 gatatgcgca tggatcagtc agctccttta tccgcttatc acgtaattaa tgaatattcc
    25381 taccatgaat tagtaaagat tttcttccgc tatggcgagg agaaattttc caaacaaatt
    25441 gctcgtgaaa tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc
    25501 gaaatcatta aacaagtcat cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa
    25561 agaatttttc aagctgtgcg gattgcagta aatgacgaac tcggtgccga agaggcctct
    25621 ttagaacagg caatccggtt attgaaagtc aatggaagaa tcagcgtaat cacattccat
    25681 tcattggaag acagaattgt caaaagtatg tttaaagaat atagtacggt acaagatcta
    25741 ccaccgggac ttcctgtcat acctgacgaa ttccaaccag aactgaaagt cattactcgt
    25801 aaaccaatcc ttccttcaga ggaagagcta acagaaaaca atcgatcacg cagtgcaaag
    25861 ttacgtattg cagaaaaaat aagaatgaaa gaggagtaga atgatggcag aattaaagaa
    25921 aatgcaagat tacgaatatg atttacctga attggtggat cagccggaac aacaaatgcc
    25981 tgattcttct tcgaatcaag aaatcattct tccacaatca ccggcaaaac gattaaagca
    26041 gatttctgct ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg ttgctttatg
    26101 tgttttaaca gttatgctgc gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat
    26161 acaaacagaa atcaatgata aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga
    26221 actttctcgt actgaacgga ttaaacaaat cgcagaagaa aaaggattgt ctattaatga
    26281 cgacaattta aggaaagtga agtaaatgag tcttaaaaat aaaattcggc ggtttatgga
    26341 aaagaaaaat ctaaatccga tgaacaatcg caaaaaagta ggtatcattt tatttgctac
    26401 gagtattgga ttgttctttt tatttgccgt ccgtttctcg tatatcgtta ttggaggtca
    26461 tgttgctggt acctcattag ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt
    26521 aaaagcaaaa agaggaacga tttatgatcg taacggtgtg gcgctcgcag aagatgcctc
    26581 ctcttattca attaaagcga tactctcaaa aacatatacc tctggggata aaaaactgta
    26641 tgttgaagaa aagaattttg ataaaatagc agaaatcctg cataagaatc tttctataga
    26701 caaaaaagat gcactgaata ttttagagga cggcgcaaaa aaagagctgt atcaagttga
    26761 atttggtagc tatgggaaaa atatctccca agaaaccaag caaaacatcg aagcggacat
    26821 gaaaaaagaa ggtgtcgctg gtctttattt tgtcgaccat caggctcgga tgtatccaaa
    26881 tggcgtcttt tcttctcatt ttattggata cgcagttcca gacaaagatg agaatggctt
    26941 agttggtaaa ctaggtttgg agtctgctta caatgatatc ttgagcggta aagatggtaa
    27001 gattatttat caaaaagata attttcaaaa tcctctgcca ggtacagtgg cagaagaaga
    27061 aaaagctgtc gacggacaag atatctacac gactttagac agcagactac aaagttattt
    27121 agaaacactg atggatcaag taaatgaaga atatcagcca gaagaattga cagcagtcct
    27181 gatgaaagca aaaaccggag aaatcttggc aatgggacaa cgacctactt tcaatcctga
    27241 aacaatggaa ggattgactg gaaaagatgc tatctggcgt aatttcttgg tacaagatag
    27301 ttatgaacca ggatcaacca tgaaagtttt cactactgca gctgctatcg aagaaggaga
    27361 attcaacgag aatgaaacct tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa
    27421 tgaccatgac tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt catggtcaag
    27481 taacgtcggg atggtcatac tggaacagcg attaggcgga cgatggtata actatttaca
    27541 aaaactggga tttggacaaa gtactcattc tggattagat gatgaagtaa acggtgcttt
    27601 acctacatta aatattgtcg accgagcaat gagtgcttat ggacaagcgg taggtgtgac
    27661 gaatttccaa atgatgaaag ctttcacttc tattgcgaat aacggtacaa tgattcaacc
    27721 tcgatatatc agcaaagtgg tggatcccca aactggagaa gagcgaacaa ctcaaacaga
    27781 agttctaggg caaccttttt caaaagaaac aacagaaaaa gtccgtgaat atatgagaga
    27841 tgttgtcgaa agcgaaaact acggtattgc ttatggtgta tatagtgtac ctggatacaa
    27901 tgtatcggca aaaaccggaa cagcccaaat cgcttctgat acagggggat atcaaacagg
    27961 agatacagcc tacctgtatt cgattgtgga gatggttcct tctgaagatc cagactatgt
    28021 cttgtatcta acaatgaaac accctaagac atatgatcga atggcattgg ctaaaatcgc
    28081 aaatccattg atgaaacggg caatggattt taaagaaact gaagaagatt cagatacaga
    28141 aacaaaaaca gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc
    28201 agctgatgct caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca
    28261 aaaacaatct actgccaacg gtgatcagtt gatctctgga gaaaaactga tcctatatac
    28321 tggtggtgat aagctgatgc ctgacgtaac aggatggtca aaagcagata tcatgaagct
    28381 ggggaaaatc ctcggtattg aagttagttt tgatggagat ggttattgtg tcaaacaaga
    28441 acttgctcca tatgaaaaaa tcactgaaga caaacttagt tttactttag aagaataaga
    28501 agagaaatag gagagaaaag agcatggaat ggacacaagc attgattccc attgtcagca
    28561 gttgtgcaat gacaattgcc gcaatgccgc tatttattgg ttattttcaa atgaaaaagc
    28621 aaggtcaggc gattcgtgag gagggaccaa agtggcacaa tagcaaagcc ggaactccta
    28681 caatgggcgg cttagtgttt ctgatcggct ctattttgac aggaatttgg gttggagctt
    28741 ggcaaaaaca gctaactcct actcttttca tcttgctttt tgtattagcg ttatacggag
    28801 taatcggctt tcttgatgat tttataaaaa tattcaagaa aagaaacatg ggactgaact
    28861 cgaagcagaa attgattgga cagattctag ggggaatcat cttttatctt gtttaccgtt
    28921 ctgaaggtta tccaggtgta ttgaatttct tcggactaga tctaccttta ggaatcattt
    28981 atggtatttt cgctctcttc tggcttgttg gattttcaaa tgcagtaaat ctgacagatg
    29041 gcatcgatgg attagttgct ggcttaggaa gtatctcttt tgcagcttac ggtctaattg
    29101 cttggcatca gcatcagtat gatgtattaa tcatttgctt aagtgtttta ggaggtttac
    29161 tgggtttctt cgtctacaac agaaaacctg ccaagatttt tatgggagat gtcggttcat
    29221 tagcgttggg cggactttta gcggcaattt ctataatgct aaaccaagaa tggacattgc
    29281 ttttgattgg tttgatctat gttatggaaa cagcaagtgt gatgcttcaa gttacttcct
    29341 ttaagcttac aggaaaacga atttttaaaa tgtctccaat tcatcatcac tttgaaatgt
    29401 gcggatggtc ggagtggaag attgatacag ttttttggtt gatcagcatt gtgacttcgt
    29461 tgatcacatt atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat
    29521 gaaaataaga aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta
    29581 ttacatgaat tgggcgcact tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg
    29641 gaagcacaag agctgctttc tttagggata aaagtaataa caggtagtca tccaatcgaa
    29701 ttgttggatg aagaattttc attgatggtc aaaaatccag ggattcccta cagtcatcct
    29761 ttagtagcaa aagcccaaga aatgggtatt ccggtaatta cggaagttga gctagcttac
    29821 gaagtagcgg aatgcccaat tattgggatt acaggaacga acggaaaaac aacgacaaca
    29881 acgatgacag gactactact gaatgcagga gcagatcaag gaattgcacg tttagcgggt
    29941 aatattgggt atccagcaag tggtgtagcg caagaagcaa aatcagaaga taaaattgtg
    30001 atggaattgt ctagcttcca actcatggga atcacagatt tccgccctca tatagcggtg
    30061 attaccaata tctatgaagc acatattgat tatcatggta caagaaaaga atatgtcaaa
    30121 gctaaatgga accttcaaaa aaacatgaca gaaaaagatt atctgatcct taactggaat
    30181 cagtcagaac tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca
    30241 aaagaagtgt tggaagacgg tgtgtatgct gacgattact caatatacta taaaaaagaa
    30301 aaaataatgg aaatatctga acttggcgta ccaggtaagc ataatgtaga aaatgcacta
    30361 gctgctattt ctgtagcaaa attgtatggt atatcaaatg aagctatacg agaaacgttg
    30421 cattttttcc atggcgtacc acatcgtacg caatatgtag gagaaatcca aggcagaaaa
    30481 ttttataatg attcaaaagc aaccaatatt ttagcgacta aaatggctct aagtggattt
    30541 gaaacgtcta aggtcgtgtt attggctggc ggattagaca gaggaaatac atttgatgaa
    30601 ttaattcctt cattaaaagg aatcaaagca atggtcgtat ttggccaaac aaaagaaaag
    30661 ttgatggatg ctggaaaaaa agcaggcatc gagacaatag taacagccga ttctgtagaa
    30721 caagcagttc ctttagcatt agaaaacagt acagatggtg atgtagtttt attatcacca
    30781 gcgaatgcca gctgggatca atacccaaat tttgaaacta gaggaaacag attcatggaa
    30841 gctgtgaacc gattaaaata gaaaaagtag aagcgagcga taaaaaatga aaattttggt
    30901 tacaggcgga ggaacagggg gacatattta tccagctctt gcctttgtca attatgtaaa
    30961 gactcaagaa ccgaatgctg aattcatgta tgtgggagca aaacgaggtc tagaaaacaa
    31021 aatcgtccct gatacaggaa tgcctttcca taccttggag attcaaggat ttaaacgaaa
    31081 aatttccatg cataacatca agacggtccg attgtttctt aagagtatcc gtcaagcaaa
    31141 aaaaatcctc agtgattttc aaccagatat cgttatcggt actggaggat atgtttccgg
    31201 agcggtagtg tacgctgcag ccaaaatggg tattccaaca gttatccatg aacaaaacag
    31261 tgtaccaggt gttaccaata aatttttaag ccgatatgtg gatcggatcg ctctttcatt
    31321 taaagatgcg gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc
    31381 acaagaagta gcagatacta aaaaatcaga tattttgaaa acatttgatt tggatccaga
    31441 aaagaaaaca gtcttgattt ttggcggtag tcaaggagca cttaaaatca atcaagcagt
    31501 atcagttttt ttaagtgaat atattccttc ttcttaccaa gttttgtatg cttcaggaga
    31561 tagatattat gaagagatcc taaataaaat aaatcctgaa atacaaaata ttagtgtcca
    31621 gccttatatc aaaaatatgg cagaggtcat ggcttccagt gatctgctgg tggggcgtgc
    31681 tggtgcaact tcgatcgcag aattcacagc tctggggtta ccggcagtgt tggttcccag
    31741 cccatatgtt acgaatgatc atcagactaa aaatgcaatg agtcttgttc acgcaggagc
    31801 agcaaaaatg attgcagata atgaactaac aggtgaatca ctaagtcaga cagtcaacga
    31861 aatcatgggt gatgaagaat tgcaaaagca aatgtgccga gcttctaaag aacaaggaat
    31921 ccctgatgct tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa aataaaggcg
    31981 gtggtcttag tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa
    32041 aacaatttga ctccttggca aaaggctaac cgaaaatatc tggcagaaca agagatgaaa
    32101 aaccaagagc caaaaaaaga agacataaca ttagatacac ccctttcaga ttctgaagaa
    32161 gaaaaaacgg ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt
    32221 tttgaagaga tcaagcaaaa gaaaaacggt ggaccatata acggttcttt tttgaaccgg
    32281 ctgcccaatt taaaaagtca acggcacaaa gtactatttc gtcggttagc tttgatcatc
    32341 actgtattgg gtataccgct gatttttttg atctattatg tttctcctta tagcaaatta
    32401 caagcagttg cagtatcagg caataagaca gtcaacagcc aagaaatcat ctctgataca
    32461 aaattgtcat taggagaaaa tgtttgggga cagtattttc atcgatctac ttatatcgat
    32521 cgccttaaaa aagcacagcc aagaatcgaa acagcaaata tacatttcaa agggatgaat
    32581 gaattcgatt tggatgtaac agaatataaa gagattgctt tgatcgcaaa aaacaatcaa
    32641 tactacccag tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa
    32701 aatctcccta tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa
    32761 tacaatcagt tatcatcaga attacaaaaa gcaatttctg aaatcaagta cacgccaaga
    32821 gcaagcaata aaaatctgat ccaattgaat atgaatgacg gcaatcaagt tattgtaaat
    32881 atcaataatc ttgcgaacca aatgaaatat tattcgcaag tagccaaaga tatggatgaa
    32941 aaaggtgtga tcgacatgga agttggtatc ttctcgcatc cttacggtag cactcaagaa
    33001 acggaagaat cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca
    33061 aacgaatcaa gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc gtctagcaca
    33121 aacggatcat ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga taatccaagt
    33181 acaactccgt caagtttaaa tgaaaattga taaaacaact ttaaaaagta agaatgtact
    33241 ttctaaaaac cagtaattta tggtagaatt ggaagcagtg aatatatcta ttaataagta
    33301 tattttaaaa cggacgctag ttgttcgctt gtatatggaa tagtaagtag gagggaatcc
    33361 cattcatggc aaaaacagga atgtatgtag gccttgatat tggaacgaca tctgttaaag
    33421 ttgtcgtggc tgaatatatc gacagccaaa tgaatattat tggagtagga aacgcaaaat
    33481 cagaagggat taaccgaggc attatcgttg atatcgataa aacggtccaa gcgatacaac
    33541 gagcagtacg acaagcagaa gaaaaagcag gtatccagat caaaggcgta agtgtcggtt
    33601 tgccagcaaa catgcttgaa gtagaaaatt gccagggaat gattgcagtt aacggagatt
    33661 caaaggaaat caccgatgaa gatgtcagaa atgtggcttc agctgccctt gttcgttcaa
    33721 tacctcctga acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc gatgggttcg
    33781 agggaatcaa agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt
    33841 ttacaggacc taaaacgatc atccataaca tacgtaaatg cgtggaaaat gccggattaa
    33901 tcgtcaacga aatggtgatt actcctttag cgctgactga atcaattctt tctgacggag
    33961 aaaaagattt tggtacgatc gtcattgata tgggtggcgg ccaaacaaca acggctgtta
    34021 tgcatgataa gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta
    34081 aagatatctc tattgtatta aatacgtcat ttaacaatgc agaagcgttg aaaataaact
    34141 acggggatgc atacccagaa cggacatcac cagatgaaga atttccagta gatgtgattg
    34201 gtcagtcaga accagtgaaa gtcgatgaac gctacttgtc agaaattatt tcagctcgaa
    34261 tggaacagat tttcaacaaa gcaaaagatg cattggacca aatcgaagca ttagagttgc
    34321 caggtggcgt cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg gacttggcac
    34381 aagaaatctt cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc
    34441 cggtatttac aaatgtaata agtatcgtgg attattcagc aaacttgagt gaagtgtatc
    34501 aattggcaaa aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac
    34561 aagaaacaac aaacacatat gagagctatg aagcacctga agaaacaatc tacgatgagc
    34621 cggaaccaaa agagtccgga gaaaatgtaa aaaataaaat caagggtttc tttacaaata
    34681 tttttgatta attcatcctg ggaggaataa gatatggaat tctcaattga taataatatc
    34741 aatgacggcg cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac
    34801 cgaatgattg aagaaaacgt taaaggtgtt gaattcatta cagccaacac agacgtgcaa
    34861 gcattaaaaa attcaaaagc tgaaacagtt atccaattag gccccaaata tactcgtggt
    34921 ttaggtgctg gttctcagcc agaagttgga caaaaagcgg cagaagaaag tgaacaatcc
    34981 ttacgcgaag ctttagacgg tgcagatatg attttcatca ctgcaggaat gggtggagga
    35041 accggtacag gtgctgcacc aatcgttgca ggtatcgcga aagaattggg cgcattgact
    35101 gttggtgtcg tgacacgtcc attcactttt gaaggaccga aacgtggccg ttttgctgca
    35161 gaaggtatcg ctcgtttgaa agaaaatgtg gatacattat taattatctc aaataaccgt
    35221 ttattagaag ttgtagataa aaagacaccg atgcttgaag ctttccgtga agcagataat
    35281 gttttacgac aaggtgtaca aggtatctct gatttgatca cagcgccagg ttacgttaac
    35341 cttgactttg ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt gatggggatc
    35401 ggtgtggcta gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca
    35461 ttgcttgaaa catctattga tggagctgaa caagtacttc ttaacatcac aggcggtttg
    35521 gatatgacat tgttcgaagc acaagatgct tcagatatcg tagcaaatgc agcaactggc
    35581 gatgtaaata tcattctagg tacatctatc aatgaagaaa tgggcgatga aatccgtgtg
    35641 acggtcatcg caacaggtat cgatgaatca aaaaaggaac gcaagtctag ccgaccagct
    35701 agacaagcac aaatgcaatc acctgctcaa aagactgtat tagatatgga tcaagctaaa
    35761 ccaacttctt cagaagaaga aaacagtttt ggcgattggg atatcagacg tgaacaaaat
    35821 gttcgtccta gagttgatga ttccaatttt gacaatatcg aaaaaaaaga atttgataca
    35881 ttcaaccgcg aagaaacaaa atctcatggc gatgatgaat taaatacgcc accgtttttc
    35941 cgtcgtaaaa aataaaggag aggaagagca catgattgct gacaacttgc agaaaatcaa
    36001 gcaagaaatt caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt
    36061 agctgtaaca aaatctgttg aatccgctca agctgaagaa ttggttagag ctggcgttat
    36121 gaattgtgca gaaaaccgag tagataaatt attagaaaag aaacaagatt tatttgattt
    36181 tcctgaaata aagtggcatt taattggtaa tttacagcgc agaaaggtaa aattgattgt
    36241 aaatgaaatt gattactttc atgcgttgga cagtttaaga ttagcagaag aaatccagaa
    36301 gcggttagag aaagaattat cctgttttat tgaagtaaat gtttctggtg aggaatcaaa
    36361 acatggaatt tcaccagatg agcttttacc gtttgtggaa tctttagctg cctgcacaaa
    36421 aatcaaggtt gttgggttga tgactatggc gcctaaagaa gcttctgatg agcaaatcca
    36481 taaagtgttt ggtacgctga aagaattgcg tgatcttgtt caagcaaaag gttatgccca
    36541 tgcaccatgt agtgaattaa gtatggggat gagtcaagac tttcaaattg cgattgaaga
    36601 aggtgcaaca tttatccgtg taggaacagc gctattcaaa gacgaataaa ggaggaacaa
    36661 aaatgccgct tttaaacaga ggagcaattg ctaatttttt cggattagct gatgaggaag
    36721 agtacgaata ccaagattat cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa
    36781 atacaacaca gaatcaatca actcattctg tatctgctgc gaatactcat gaaaaacaaa
    36841 aaacggcgac ttcacgtcct gtaacgcatg aaagtattca gccggctgaa aagaaaaaag
    36901 taattgaact acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa
    36961 cctctacaca acctaaagca aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt
    37021 attctgaagc tatggcgatt gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt
    37081 ttcatttagt agaagaaaaa caagcgagaa gaatcgttga ttttctaaca ggaacagttt
    37141 atgcactaga tggagatatc aaacgtgttg gagatgagat ttttctttgt acaccgactg
    37201 ggatggagat tgacagttca accgctcaat cttttgcaga tgcgaatttg tttgatctgt
    37261 aagaggagga actacgattt atactattgc actattactt catttacttc aacgggcagc
    37321 ctatttctat agcattatct tgatggtata tgcattgctc tcttggtttc caggcgggta
    37381 tcaatcaggg ctaggacgat tattagctaa gatctgcgaa ccgtatatca gtttgttcga
    37441 tcgtctgcct ttgaggattg gacccttgga tttcacaatt ttaggggcaa tcctgatctt
    37501 gaacttagcc gtacaaggtt tagcacagat cgttatatgg ataactgtcc atttagttta
    37561 ttgattgaag caacaaaatc tttattctca gaatagatag gaggggaaaa ttttggatgc
    37621 aaatgtatac cagcactttc gggcagatga acgtacattt atcgattcgg taggcgattg
    37681 gattgaacag gttcaaggac aatatgcacc ctatctgacc gaatttcttg acccaagaca
    37741 atcctatatt ttagaaacac tgattcgaca aagcagtgat ttgcgtttca tgttttatgg
    37801 cggatatgaa caggcagaaa gaaaaagatg tttgattttc cccgattatt acgaacctgt
    37861 agaggaagat tttgaaacag cattgtatga aatccattat cctagcaaat ttgccagcct
    37921 aagtcatggg aaaatccttg gaactttagt gggaacgggg atcaaacgct cttactttgg
    37981 cgatatcatc tcggacggtg atcgctggca agtatttatt gcaaatgagg tggatcattt
    38041 tgttgctgca caagtaacaa agattggaaa ggttgctgta cgcttggaac caatcaaata
    38101 cacagaaatg attatgccga aagatagctg gacacaagaa agaggaacag ttagttccat
    38161 gcgactagac agtgtgatct ctgagattta taatatttcc agacagcggt ccaagcagct
    38221 gatcgaagct ggtaaagtaa aagtgaactg gacagaaaat acaagacctg attttgtact
    38281 tgaactatta gatattattt cagtcagagg ttatggcaga gtccagatcc aagaacttga
    38341 ggggaagacg aaaaaagaaa agtacagggt actgttcggt gtattacgta aataaaataa
    38401 aaaggtggtt tactcatggc tttaactcca ttagatatcc aaaataagac attcccgaca
    38461 aaaatgcggg gatataatca agacgaagta gatgatttct tagatttagt tgttcgtgac
    38521 tatgaggaat tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa
    38581 aaacttgagt acttcaatga attaaaagat gctttgaacc aatcaatcat cgtagctcaa
    38641 gatacggctg ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat cgttacttct
    38701 gcacaaaaca aagcagatga gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca
    38761 actgatgcag aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa agcaagacag
    38821 cttgcgacag aaactgaaga tcttaagaaa aaaacacgag tattccatca acgtatcagc
    38881 ttgatgcttg agtctcaatt agaacaagtg aaaagtccgg aatgggatga aattttacaa
    38941 ccattctcca gctacgtgac tgacagccat gaagtgatca aagaagtttt agcaaaacag
    39001 cttgacaatg aaaatgatac tgatgtaaac tcagacacag gagtcataga agcacctatg
    39061 acggcatttg atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag
    39121 tagaacagaa aagcactttg ttattcttaa agcgagccga tgagagtgga agttcggtaa
    39181 gacactgcat ggtgaaagat cctctacttg agacctgctt ttccgaaatt agagtaagaa
    39241 gcagcggctg acctcgttat tggtcacaga gagtttgcac tagatagatg caactaaatt
    39301 tgggtggtac cacggcatgt tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa
    39361 acaaataaag gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca
    39421 aaattcccta tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa
    39481 gaaaatcgaa tctaccaaaa aagacaagag ttgaacgaag gaaaacctag ctttgttctt
    39541 catgatggac ctccatatgc caatggtaat atccatttag gacattcatt gaataaaatc
    39601 agtaaagaca ttatcattcg ttcaaaatca atgtctggct atcgtgcacc atatgttcca
    39661 ggatgggata cacatggatt gccgattgaa caagtattgg ctaataaagg cgtgaaacgt
    39721 aaagaaatgt ccatggcaga atacttggaa aaatgtcgtg aatatgcact ttcccaagta
    39781 gataagcaac gagcagattt caaacgccta ggagttgcag gagattggga acatccttat
    39841 gttacattag atccttctta tgaagctgca caagtacgcg tatttggtaa aatggctgaa
    39901 aaaagctata tttacaaagg attgaaacca atttactggt ctccgtcaag tgagtcttct
    39961 ttagcggaag ctgaaattga atataaagat gtaaaatctc catctatttt tgtagctttc
    40021 aatgtcaaag acggcaaaga tattcttgat gaagatacag cttttgtcat ttggacgaca
    40081 acaccatgga cattgccagc aaaccaaggt attgctgtaa atccaaaatt tacgtatgta
    40141 ttggtggaag ccgatggccg taagtttgtc gtagcgaaag atttgctaga aacagtccaa
    40201 tcagaaatcg gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa
    40261 tatatgactg caagtcatcc attctatgaa cgtgaatcac ttgtgatttt aggcgatcat
    40321 gttactttag acgctggtac tggattagtc catactgctc caggacatgg ggaagatgac
    40381 tatatcgctg gtagcaaata taaactatct gtagtgtcac ctgtagactc aaaaggattg
    40441 tttacagaag aagcacctgg atttgaaggt atcttttatg acaaagcaaa tcctatgatc
    40501 actgatctat taaaagaaaa aggagctttg ctgaagctag atttctttac tcatagttat
    40561 cctcacgatt ggcggacgaa aaagcctgtg atctatcggg caactccaca atggtttgca
    40621 tctatcgata aattccgtca gaatattttg gatgaagtag aaaaagtaga gtgggttatc
    40681 ccatggggta aaacacgttt gtataatatg atccgtgacc gcggcgattg ggtaatttcc
    40741 cgtcagcgtg catggggagt accacttccg attttctatg ctgaaaatgg agaagcaatc
    40801 atcacaccag aaacaatcga acatgtggca aaattatttg ctgaacatgg atcaattatc
    40861 tggtttgaaa aagaagcgaa agaacttctt cctgaaggat ttacccaccc aggttctcct
    40921 aatggcgaat ttacaaaaga aaaagatatc atggatgtct ggtttgattc tggttcttct
    40981 catgaagctg ttttacgtca gcgaccagaa ttatcattcc cagcagatat gtatttagaa
    41041 ggatcagacc aatatcgtgg ctggttcaac tcaagtatca cgactagtgt ggctatcaat
    41101 ggtgtagcac catacaaatc agttatttct caaggatttg ctttagatgg tgaaggccgt
    41161 aagatgagta aatctttagg aaacatcatt gcaccagaaa aagtgatcaa acaatttggt
    41221 gcagatatcc ttcgcttatg ggtatctagt gtagattatg aagcagatgt tcgtgtttct
    41281 atggatatct taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc
    41341 ttattagcga atacagaaga tttcgatcct aaaaaagata ctgtttcgta caatgattta
    41401 cgttctgtag acaaatatat gaccgttcga ttgaaccaag taatcaaaga aatccgagaa
    41461 gaagggtacg acaaatataa cttcatgcat atttaccgta cagttatgaa cttcttgacg
    41521 gttgatctat cgtcattcta cctggatttt gctaaagatg tagtttatat tgaagcagaa
    41581 gatgattatc aacgtcgttg catgcaaact gttttctatc aaacagctgt agctttgaca
    41641 aaattattga ctcctatcat tccgcataca gcagaagaaa tctggtcatt cctgaaagaa
    41701 gaagaggagt acgttcagtt aagcgagttt cctggttatg aagagtttgc aaaccaagaa
    41761 gaattgatgg atacctggac tgctttcatg gacttccgtg ataatgtctt aaaagcattg
    41821 gaagaagcaa gaaacagcaa attgattggt aaatctttgg aagcaaaact aactgtttat
    41881 ccaaaacaac aagttcgtga aatgttaaaa gctttggatg cagatattgc acaattattg
    41941 atcgtttcac cagactactt tgaagttatg gcagcagatg aacaagtgcc agataacgct
    42001 atggtatttg atgatgttgc tattacagtg gaaaaagcag acggggaaac ttgcgatcgc
    42061 tgtcgccaag ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt
    42121 tgtgcaaaaa tcgttgaaga cttctatcct gaagctgtag ctgaaggatt cgaagaaaag
    42181 taaaagggaa aagggggtgt gacatgttct ttgtcacacc cccttttgtt atcaatcgtt
    42241 taattttcca cgttcacgat accagttatc tggttgccag atccattcga atccgtcttt
    42301 ttcaagtagt tcgaaagctg ctttaggtcc cattgatcca gctttgtagt tgggaaaatc
    42361 cggttgtgtt tggtcccaag cttttcgaat gacatccacg atatgccaag attgagcgac
    42421 ttcttcccaa tgagagaagt tcgttccatc accattcaac gcatctaata gtaatttttc
    42481 gtaagcttct gggctatttt caaccatttc tgcactattt ctaaattcca atttgattgg
    42541 ttccgtttca aatccttgcc ctacttcttt cccattcaag gataaagaaa atccttcagt
    42601 tggctgaata tagatcgtta gaacattagg tggaagtgtc gtatcgtcac atggttcatc
    42661 gacagatgtt ttgaatacat taacaggaac ttgtttgaat acgatgttga tccgagtccc
    42721 tttttcagtc aaacgtttgc ctgtacgtac atagaaagga acacctgacc aacggaagtt
    42781 atcgatagtg aatttgcctg cgacgaatgt ttcagtctct gattgagcat ccactttgtc
    42841 ttcttcgcga tagcccttga attgtacacc atccaattca ccttgagcat attgcccgcg
    42901 aacgaagttt tctaacactt cttcttcgtt ataaatgcgg atagctttca atgctttgat
    42961 cttttctgta cggatttctt tttcggaaaa tgctactggc ggttccattg caagtaatgc
    43021 tacaacctgt aaaatatgat tttgcaccat gtctttaagt gcgccactat gatcgtagta
    43081 gcccccgcga tcttctacac cgaggttttc gccaaaagta atttggatat tgtcgatata
    43141 gcgattgttc cattgagcct caaaaatatt attggcaaaa cggatagctg agatgttttg
    43201 gatcatttct tttcctaaat aatgatcaat cctgaaaata tcttgttctg ggaatacttc
    43261 tctgatttgc tggttcaatt caaaagcaga ctggtaatca gaaccgaacg gtttttctat
    43321 gatcaaacga ttgaagccat tttttgtcat aatattttgt gatttcaaat gagaaacgat
    43381 cgttccaaaa aattgaggtg ccatagccaa ataatagaca cggttaccgt ttaattgata
    43441 gtgtccatct aatcggtcag acaattcttt taatgtctga taatgcgttg aatcatttac
    43501 atcatgggat tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt
    43561 caatgcacgg attgtttctt tgacaacttc acggtagtgc tcatcgctcc agggacggcg
    43621 agcggttccg attacagcaa aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa
    43681 agacgggtac aatttacgct gggctaggtc acctgtaccg ccgaatatcg taaacaatac
    43741 atttttttca ttagacatgt ggatacactc ctttatattt tatcgacaaa aatcgtgctg
    43801 gccgccttaa agctgacggg gattttcttt ctatcatttt ggatagtgat cggtccttca
    43861 taagctgcga tgtcaacgat ctcatattct tcgttgatat tcaggtcaat agtgactaag
    43921 taatccaaca attctttttc atcaagcact cgtgcgatac gcacttttgt accgacagga
    43981 taggactcca atgtttgtcg gcgttcttca tggacaactt cctctttttt aggaatcgat
    44041 cctccatgag gacaatgttc aggatgattc aaatagtttt ctaaatgatc tgcaagatgt
    44101 tccgaagtca catgttccaa tacttcagca tcatcatgga cttcattcca gctatagtct
    44161 aaatgttcaa ctaaaaaaac ttcccataag cgatgtttgc gaatcagact gctagctttt
    44221 tgcagtccgc ttgcagtcaa ttgtacacct tgataagggg aatgttctac aagctcttct
    44281 ttcacaagtt tagaaatcat ttcactgaca gatgcagctg agacatctag actagatacg
    44341 atttgtttat tgttgacttt cgttgtatct ccacctagtt ctaaaatgat tttcaaataa
    44401 tcttctcgat ttggtgtcat attcgatcat cctttcatgg taaaaaacca tcattagtaa
    44461 gtgtaacaaa aaaaacgtta attgactatt tttctaaata aaaaacaaag gatttttaga
    44521 cgtttttaca acaaaaaacg aaagatgata gagaagactc attctttcgt ttataaagga
    44581 tatatatata cattgggagg gatctatttt tcagataaca acttatatga agtgtctgca
    44641 gattcgtcat aaagagtttg ttcgggataa gaaaagataa gataaaaacc atactgatca
    44701 cgtacgacta aacgatattc atctatttct accacagatg tgccaagaga ctgaccgttg
    44761 atcaaaaacg tgtaatcaga tgtaatctgt aattgttgtt tttgctggct atcactaaag
    44821 aaccatgtac cttgatactt tgagagtcct tctgactttt ccgcttgttt tttctttttc
    44881 aagtaaataa aacttgagag aacggtaatg agtgaaccaa gaaaatagat catttttttt
    44941 atcaccaaat cactccttta tgagaaaata atcgcataga catattcttt cttctttatt
    45001 tcattgtacc gaataaaagc taaaatttct ctaagacttt cgactggttt ttttactgtt
    45061 tttctacaaa ttatgatagg atacatagta ggaagaaagg tgacataatt tgaagtatca
    45121 gcaagtggaa atagcttttt ttattgaccg cccgaatcgg tttatagcac attgtatgaa
    45181 aaaagacgga gaaataatta ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct
    45241 gataccaggc gctgaagtgg ctattgtatt tgctcctgga cctaaacgga aaaccgccta
    45301 tgatctgatt gctgtaaaaa agaagggtga ctggtttaat atcgatagtc aattgcctaa
    45361 tcggctggca attgatggta ttttagacgg cacgatcctt ttaccaggat taactggtcc
    45421 atttacggta tacaagcgtg aagtaactta tcttcactcc aaatttgata tttatttaga
    45481 gacggtggtc ggacaaaaag catttgttga agtcaaaggt atgacacttg aaaataaagc
    45541 gattggtgct tttcctgatg cgcccacact cagaggattg aaacatatag gagaattaac
    45601 gtatgccgca caagatggtt atgccgctta tgtcttgttt attgctcagt tcgaacatct
    45661 tcatctggca acgatccatg aagaaatgca accagcacta gctgatatgg ttcgacatgc
    45721 gcagcaaagc ggagttcaaa tcctcgccta taattgtcaa gtatcggcag atgaggtaac
    45781 tgtcaagcag gccattccat ttgatgtaga tatccctttt gaagatccta tacaatagaa
    45841 agaaggtaaa ataatgatca acttaggtat tatcggaaca aattggatta ctcatcaatt
    45901 tgtccaagca gctttaagca caaaaaaata tgaattgaca gcggtatatt ctagacattt
    45961 ggaaaaagca caggaatttg gttctcatta tgaaggggat atcgaattcg caacagattt
    46021 gaagactttt ttcaatatta ctcatatgga caccatttat attgcttctc cgaatagtct
    46081 tcactttagt caagctaagc aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc
    46141 agctttttcg acgccaaaag aaatggatga aatcatccat ttggcaaatg aacacaatgt
    46201 gtattttttt gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta
    46261 cttaccatta aaagacgaga taatcggtgc aaatttcagc tatatgaaat attcttcgcg
    46321 ctatgaccaa gtattagatg gagaagaacc gaatatcttc tcgactcatt tctcaggtgg
    46381 tgcattgatg gatttaggtg tttaccttgt atatgcagct gttgcttggt ttggaatgcc
    46441 aaaagaagtc cattatttcc caagaaagat ttctactggt gtagatggtc ttggatgggg
    46501 gatcttacgt tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg gtgattctta
    46561 tttaccgtca gagatttatt ttgactcagg aacgctagtg atgaatggag tcaatgcaat
    46621 tgaacaagca caatttcatg atcgcgcgca taaagaaatc attgatttgg cggtaacagc
    46681 tgcagaaaat ccaatggcag atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa
    46741 agatccagct tggggattgt tgtatgaaga gtggatcgag ctagctagaa atgtgaacca
    46801 agtgatttat gatttaagaa aaaatggcgg catcaaattc gatgcagata atgaggaaga
    46861 tttttaatga acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa atatgaggat
    46921 aaagggttta tctcaccttc tcttattcaa cgagcagttt ttcagccact aagtaataaa
    46981 caaagtatag tagcggtttc tcctacaggt tcaggtaaaa cactggctta tctttggcct
    47041 ctactactaa acgtggaacc aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa
    47101 gaactagcta ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa agagttgaaa
    47161 gtccaatctt tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa
    47221 ccagaaatct tgattggtac accaggaaga atattggaat tgatgaaagc aaaaaaaata
    47281 aaagcacatc aagtgaaaac aatggtattt gatgaagcag accagctatt tgacgctggg
    47341 aacagccaaa tcattgatca aattcttcat caagctccaa cagagtatca gctagctttt
    47401 ttttcagcga cagcagatcg ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt
    47461 gagacaattg acgtgacagc agaggacgat tctcgaaaag gactgagaca ttacttttta
    47521 cgtgtaccga tacgaaagaa agatgactac ttgagacgtc tcacgcatat agaagatttt
    47581 catggtctga ttttctttaa tcaactgact gaattaggcg taatggaaga aaaattgctg
    47641 tatagaggtg taccggtagg aagtctggcc tctgatcaga acaagctact ccgaaaagca
    47701 gcgctagaac aattcaaaaa gaaaatggtc agagcacttt tgaccacaga tgtggcggct
    47761 agaggtttag atattacagg tcttccttat gtagtgaatg cagaagttcc gttaagtgaa
    47821 gaagcttatt tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt
    47881 acactcgtac aagataataa tctaagagat ttaaaacggc ttttacgtcc atcaaatata
    47941 acacttgaag agattttttt atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa
    48001 gatgcagctc ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa ggagcagaac
    48061 ccagaatata acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga
    48121 aataaaggag caaggcgcaa ataagcgcct ttttatgcta tgattatgaa aacgggaaca
    48181 aaattggttg ccagaggagg ggttatgtga aagaacgtca agaagagatc ttgcagctat
    48241 tgacagaaaa ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta actatcgatc
    48301 gaagcaatgc aagccgatat ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg
    48361 ctggccgacc agtcgtttac tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg
    48421 tcgactcttc tacgcaagtg acttttgaaa cactcgttgg agaaaatgat tctttaaaag
    48481 tcagcataca gcaggcaaaa gctgcgattc tttatccacc gcgggggttg catacgatta
    48541 tttttggaga aactggtaca gggaagtcga tgtttgctga atgtatgtat cattttgcaa
    48601 tcgattcaga gatgctttct gcagatgcgc catttgtttc tttcaattgt gcggattatg
    48661 ctcaaaatcc gcaactgctc tttggacaca tctttgggat caaaaaaggg gcttacacag
    48721 gagcagctca agatagtccc ggtctaatag ccaaagcaga cggcggtata ttgtttttag
    48781 atgagatcca ccgtttaccg ccggaagggc aggaaatgct gtttactttc attgataaag
    48841 gtatttaccg tccgcttgga gaaagtgcac aagtacatga agcatctgtt cagatcattg
    48901 gggcgacgac tgaatcttca gaaagtttct tatctacgtt caatcgacgg attcctatgg
    48961 caatcacctt gccgaatctt gcttcacgtt cactggatga gcgttatgaa atcatctctc
    49021 tttttatcaa acaagaagcg aatcgtttga atcaacgtat cgatatcgaa aaagaagcga
    49081 ttcttgcctt catgctttat gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga
    49141 aactagtctg tgcaaaatct tttttacatt accgtactca tcatgagaaa aagctaatca
    49201 ttcgaaaaga agaactttcc cttcaagtac aaaaaggatt actgaaaatc aaggaaatac
    49261 cggaacgtct cgaccgattc atggacagca aaagtcagta tttgacattt gaaccaggat
    49321 ttgctgatgt tgtctggtca caagatccag aacgaaacat gcaggtctat aacgatattg
    49381 aggaaaaagt cacttctctt tccgaaaccg gtgtggagaa cattgatcta gagaccctca
    49441 tttcaaaaga tgtggatgct tatttccaga catatgtcaa agaattgact aaaagttcga
    49501 ttcccaaaga tcttttgcca gatgaaatct ggcggttaac caatcaattg tatgatgtgg
    49561 ctgaaaaacg gttagaccgt atttataatg aaaaagctcg ttttgctttt gctttgcatc
    49621 tgcaaagtac attggatcgt gtgagagaag gtcatatgat cgtccaccca gatttgaata
    49681 acgtacggaa aaattttaag agggaattcc aggtagcaat cgatctttca acgatcattg
    49741 aagaagaaga acaagttgag attccttttg atgaaatcgg ctttattagt atgtttcttt
    49801 ctatcaatct aggagaaagt gagcctgtta aagaaaacaa agtagatgtt ctcatattga
    49861 tgcacggcag acagactgct tcaagcatgt tggagatggc acaagaacta ttaggaacaa
    49921 aagtagggaa agcttttaat atgccactgg ttatggaagt tcaaacaatg tatgaagaag
    49981 tgttacaata tgtcaaaaac tatcaagagc atttgacgaa tggcctactc ttgttaacag
    50041 atatgggatc attaaacacc ttcagtgatt tgatttatga agagactggc attcggacga
    50101 aagctatcac gatgacaagt accatgattg tgatagaagc gatacggatg gcaagtgttg
    50161 gaagaagctt agaagatatt tatcaaaata tccagctttc ctttgaatcg atcgtacgtg
    50221 atcagttcaa agcggaaaag tctgaaactt tacagcgtaa aaaagccgta atcgtgacat
    50281 gctttactgg tgaaggagta gctgccaaat tatatcagcg tattatgcca gtgattaatc
    50341 aaaataaggt agaactgatc cagatgcagt ttttagaacg tgaatcattc aaaaaacaca
    50401 tcgatgcctt attggaagaa tatgagatca aagcaattgc gggaacagtt gatgttgatt
    50461 atcaaaatat cccatttttt tcagcctatg acgtttttga tgatgaaaaa ctaaatattt
    50521 taaagcgtat agctgatgat gagatcccga cagctaaaat tgtcgattca cttgctggat
    50581 cattaagcga tgtcttttct attgatgaac tgatccgttc attgcaaaag acagttcagc
    50641 agatccagac cgatttgtcg attatcgtgg aacctggagt agatactgga atcgttatcc
    50701 atttagcctt catgttagac ggattgatca aacaagaaag ccatcggcaa tttgagaaac
    50761 tgtctatttt ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca aacttgatgg
    50821 cactagaacg aaaatatcag gtaaaaattc cagaagatga tatcgcattt cttacacaaa
    50881 tgtttcttga aaataaaata aaaaaatgaa cacactaatc atactgttta cacactaaac
    50941 atctgtgtgc gcactataaa aaagagctaa aaagcatgaa aacgctattt cagctctttt
    51001 ttttattggc atgcttcttg cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa
    51061 atggcaaaaa aaacaatcat gttagtttgt tcagcaggga tgagtacgag tttattggtc
    51121 acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag ccgatatttt tgcggtctct
    51181 gcctcagatg cagataataa tttagaatct aagaatgtcg atgtattatt attaggtcct
    51241 caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg tattccatta
    51301 gatgtcatca atatgcaaga ctatggcatg atgaacggag aaaaagttct tgcacaagca
    51361 gaaaatctaa tgaaataaat caagtatgca gttgggggta taacaacgtg gaagatcaac
    51421 aaaatctaga agccatcatg gggttaatca tgtttggcgg taatgcaaag agcgatgcaa
    51481 tggaagcgat tgcggctgct aaaatcggtg attttgagtt agcggatagg aaaatcgctg
    51541 atgcagaaga atcacttata caagcgcatc atgcacaaac agacatgttg acacaagaag
    51601 cacaaggaaa tcatatgcaa gtgacattat tgactgttca cagtcaagat cacttaatga
    51661 catctattgc gtttactgat ttagcaaaag aaatcatcga actttatcgt cgcgtataca
    51721 gcgaataaat acaacttctt ttttgaacgg aagaagaaaa atttttgaac ggatttttta
    51781 ttttgttata aggagaactc tctttatata aaaaacttat aggaggaagt aaaaaatgaa
    51841 cggactatca agttggatgg agaaatacat cctgccggtt gccgcaaaaa tcggagcgca
    51901 aaaacattta gttgctttgc gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg
    51961 atcagtcgct gtaatgatca atgcaattat tcgtgattta ccgcaacagt ttaacagtac
    52021 ctatgctgac agtttagcag cagacaaagg aatctttgcc gtattgaatg tcatcatcgg
    52081 aatcaatggt tttgtctgga acggtacact agcaatcgcc ggattgatct ttgccttttc
    52141 ttgggggtat aatcttgccc gagcttatgg agtaaatgat ctagctgggg ggatcgttgg
    52201 tttagctacc ttgattcaag gtatttcctt tggtccagca ttatcagcag cattagatgt
    52261 gaatgttcct tcaaatgtcg cagatgcaat caatagttct gagattggtg caacgattgc
    52321 agatggcaca ctgtcagttg gattatgggg ttggctaaaa ctagatcatt tgaatggaaa
    52381 cgcttatttt acagttatga tcatgggagc tatttcagtc attatttttt gtaaattgat
    52441 gcttgccaac attacgatca aaatgcctga ttcagtacca ccagcagtat ctaaagcatt
    52501 tgcagccatt ttaccagcga cgatcgcttt atacgtcatt gctattatca attatatcgt
    52561 aggtcgtgta gctgatgggc aactgatcat tgatttggtg caaaaatata ttgcagaacc
    52621 attcctagga ttatctcaag ggttgggagc agtattgatc gtcacagtat ttgttcaagt
    52681 attctggttc tttggtattc atggaccaaa tgtattggca ccagtgctag agggaatctg
    52741 gggacaagca caactaatca atattgatat tttccaaaaa ggttataacg gaaaaactgg
    52801 tacaccagcc gtgttagatg caatcgaaga tggaaaagct tatatgtggg tccgcggatc
    52861 gttcgatgct tttgcatggt ttggcggatc aggcggaacg atcgtcttac tgatagctat
    52921 cttgattttc tctaaacgtg cagattatct aacagtagct aaattatcca ttgggcctgg
    52981 tatcttcaat atcaacgaac cagtgatgtt tggtctgcca atcgtattga atgcaatcat
    53041 gtttatccca ttcttgattg ctccagtagt agcaacagca attggatgga cagctactta
    53101 tcttggccta gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc cgttcttatt
    53161 gtcattctta gcaacaggag cggattggcg ggcaccgatc gtaacattag tatgtatggt
    53221 cgtgactttc ttgatttggg caccatttgt catctctgca aacaaaatgg accctgccgc
    53281 aggcgaacaa caataagatg aacgtgagga gcgggacgct ttaagtttcg ttcctcataa
    53341 tgaattttcg aggaggaact gaaatgatta caattgccag tgcaatgcaa caagatgaaa
    53401 tcaaagaatt gttagaagga tacaatgaag aaggaatgac ttttacattt aaagaaaaac
    53461 aaggaatcaa gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag
    53521 caaaggaact aatcaaagca cagccttggg gaacagtcct ttattttcaa tcaacagctg
    53581 tttagaaaat ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt ttatttttat
    53641 gtagtattgg ttctttgatc gtgttgtata gtttgaatcc acattcgcaa aggtatgatt
    53701 tgtatagtat ggtcacaggt ctttttctaa caggattagg aatctatttc ttcatgaagg
    53761 ggaaaaaaag agaagaagca aaggagaaaa ataactaatg gaaatcacga aaaaattaaa
    53821 tattccagca tcgttttttt acgataaagt tatggactct gtccttttcg atgtacgtaa
    53881 agcgaccggc aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt
    53941 ctcgcaaaat aaccgggctc gcataaaaat cgaagaagtc attgaaaacc agtcttataa
    54001 gtttcgtaca tcgacaacga agaatgaatt cgtcgttcat tatcaaatca aaccattgga
    54061 cgattcgacc tgtgaggttc attacacaga aaaaatggag tcttatggca tgcttcaaaa
    54121 gttgaatgat atgcttttag gaacaatctt gatgtatttt aaacggagac gtttcaaaaa
    54181 aatgctagat atgatcgaag caacgtatta aatcaatgaa acatggtact atttagttat
    54241 tgaaaaatga agtaagctag aaggaatttt tcataggaag atagatcttt cctgcgaatc
    54301 ctcactagct tttttgtagg aaggggcagc aaaatgacaa acaaagtaag agagctaggt
    54361 gtatcttcga ttgaggaaat gtttgattta gccacttatg catttaatgg ggcagataca
    54421 cctgaaaggc gagaacgttt tcgtacactt gcggagaatt cttggaatta cggatttttt
    54481 gatgaggagg atcggctaac aagtcaagtc atggcgaccc cttttccagt gaatttttac
    54541 ggacaagagt acctaatggc cggcattggc tatgttgctt cttatccaga agcaagggga
    54601 caaggcggga tcaatcagat catggaaaag atccttgaag attgccgtga tcgtaaagta
    54661 agcttatctt atctggcgcc attctcctat cccttctatc ggcgctatgg ctatgaacaa
    54721 tcatttgata aaatcagtta tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa
    54781 tctggaaaaa taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag aagaatttat
    54841 gaacagatgc cggaaaacca acgagctggt ctgaagcgcg aagactggtg gtatacgtat
    54901 aaattcaagc aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa tgaggaagca
    54961 acgggatata tcgtttatca gcttgcaacg tcttccttta ttattgcgga gtggggattt
    55021 ttggatcatg atgctttttg cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt
    55081 gaaacatttg aatattcatg cggctacggc ggtaatgatc aaaattatct attagaaaat
    55141 ccatttgctt ctgtaatgat cacaccttat atgatggcga ggatcgtaga tatttctttg
    55201 tttttggaaa attatccatt taaaaagaga caagctgcat ttgccctaca aatagaagaa
    55261 gatcattatg cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat
    55321 catctctcaa aagtagaaca aacaaaactc ccaatcgttc gaggaagtat ccaacatatc
    55381 acgcagctat tattagggta tcgtaaagtg gaagagttgg tattccaaga aagaatacag
    55441 gtgacaaaag aatatgaaga aacgttaagc tcaatcttgc cacagcaaca gccaattctt
    55501 aatgactatt tttgataaaa aacgggtgat gacaatattt ttgtcaccac ccttttgttt
    55561 ttcatttggc atttgaagga aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt
    55621 ttttgttttc ttttgtttta gggttttctt ttttgtggtc ttggattgct tctttcgtaa
    55681 tcttttcccg atttttttta gatgaatcta agcgttctgc gtatactttt tcttttggct
    55741 tatcattcat tggaaaggac ctccctacat tatttcattc tacctctagt ttatcatctg
    55801 ttaaggacta gtggaaacgt tttgcctaac ttataagctg atttttttag cagataagct
    55861 tgcaagtaaa aaacaaacaa gctacaattg atttattgaa attgttcctg tagctcagta
    55921 ggatagagca atcgcctcct aagcgatagg tcgctggttc aattccagtc aggaacgtac
    55981 aaataacccc tgtatccttt gtgatacagg ggtttgtttt gtgtttgtgc caaaaatgtg
    56041 ccaaactagt tatcattttt atataaacta tcgaacacag cattgatttt ttctttatcc
    56101 tgatcttcaa gctctcgaac aatatgaaga taagtagaca ttgtagtttc taaactgcta
    56161 tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa aatagatgca
    56221 tgtgtatgcc ttagtccatg caaagtaaat tgcttttcaa tccctaattc ttttaatttt
    56281 ttgcgcaaat atttgctgac ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa
    56341 acaaaattat gagggttttt aacttcaaaa ttctcgaata attctttttg attgatttta
    56401 aactttttta agagatcaat cgtatgttta tcaattgaaa ttttccgatt tgaagtctcg
    56461 ttttttgttt ttccccattc atttaattta taattccatg ttttattaat agtgatgatt
    56521 tgatcttcga aatcgataac ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca
    56581 gtagcaccag caactaacac gagcatagga gaagagtact gtgcagaaag gttttcttct
    56641 acgagtttca ttaaagattt aaattgatca taatctaaat atttatcttc ttttggcttc
    56701 aatgaatctt ttcctttgat tactgctttt ctagttggat caaacggaat taatccttct
    56761 tctacggcat ctttcaacga tgctctaata tggttattaa acttaataac ggtggatttt
    56821 acatggtctt tcgcatactt atttaaaaag cgttgatatc caactctatc taaatcagaa
    56881 atcaataccg ctggcatata tttctttatg ttcattaacg tatcttcgta ttttctataa
    56941 gtgataggag atactgttcc ttctttataa agttgcatcc aatcctcaaa gtaatctgat
    57001 aataatagat ttttgcgatc ccctttaagg cctttgttca agttatattc taattcgttg
    57061 gcggcatctt ttgcttcgcc ttttgtctta aatcctgatt ttcttatctt cgtgtatttt
    57121 ccgtcatctt ttttgtatga aatttcgtat tgccaactat ttccacgttt gactaatctt
    57181 gccataattg aatcagctct ctttctctga tacaatagga actataaaga agcctactgt
    57241 ataggtttgt tttttcatag aacacgcttg ctttggacgg tgggcgtgtt tttatatcaa
    57301 ttagttatga agagcaattg aaactgtctc tgttccaaag taacctgttt gtgcttgcaa
    57361 tacattattt tgtgcatttg cttgggcttc agagatatca aaaactattt taccagtttg
    57421 ttgcatatca ggatttaagc tttccataaa gaaagagtta gtgatattac cattttcatc
    57481 ttgatttgcc gacatagatg cagccgaatc agcttcgaaa gtttttccat ctgcttttaa
    57541 tttgaagaat gagctatcaa cagtcacagc cttatcacct gcatttttta cagataagtc
    57601 aactactaag aaagtgtctt ttgcgttagt aggaagtaca gatgggccaa cttgttttac
    57661 tacttcaacg gagtttactt tgtattccat tttccctaca gcaacgtcat caccgatttt
    57721 ataagtcttt tcttcttttg ctgtagtttc ttttgtagtt tctttattag tacttgttga
    57781 tgttgcagta gctgaatctt tgttttctcc gccacttaga gcaccaccaa taccaaaaaa
    57841 caaaagtaat attaataccc aaaaccacac ttttttgtaa aaaggtttac taactttata
    57901 cattttccca tcttgaccca taactttttt tgccatttaa atattcctca tttcttgtta
    57961 taatatattt gtgatctcag aaatgaggta tgagtccgtg ttgcagcacg ggcttttttt
    58021 actgtgcata agagtatttt ttcttgaaat aagactggca aacattaaaa cattctgttc
    58081 ttaacttatt attgatagaa tagaactcca tgaaattttc taatttgaac tgagattcat
    58141 cagtcagttc gttctcaata aagatattta gtaaaatcat aattgcgatt ctatcagctt
    58201 cagtttcgaa ttttgaatga aaagttgtag agttatcgta cagtactgaa tattcaaaat
    58261 gtgaagcaat gaaatgaccg agctcgtggg ctaaatgaaa agcttcagaa ctgtcttcgt
    58321 gtagtttttc attcagaaat actattcttg gttttggata ataaaaacct ggttcttcca
    58381 tttccatata gattaacttt aaattatact cactcagcat ttctttcaac tttaaataca
    58441 tacaacccat cactccaact attcattttc ctctaaagct ttagcaattg caatcgcttt
    58501 acgcattgtc tccttagata tttcttttcc atcaaaagaa aaaacagtat cgtcttctga
    58561 taaatccaca tgtttagggg tttctttttt atctcttcct agaagatagt ctatagaaac
    58621 accaaaataa tctgatactt ttgccaaacc ttcggagtta ggtgatactg atttccattt
    58681 actgaaatat ccatttgaat atcccaatct tttttctaat tcccttacgg atattccgtt
    58741 ttgctttgtt aattctttta ttttttcgta tggattcatt gatatgtcaa cctttctaga
    58801 ctgacaagaa aaaatataga ataaaactct atttatctgt tgacaaatta gagttaaacc
    58861 tctatacttg ttcttgtaaa caaatttaac aactaaaaag acaacaaaaa acacttttga
    58921 ttattaaacg ccaaccgcca aaaaagcttt taaaatcaat gtttatatgt cttatttaac
    58981 tatgtacaaa gtatagaata aaactctatg ctttgtcaat tgaatttaga aaaaagttgt
    59041 taaatttgtt tacgaatata aaagaaagga atgagaaata tgaacacacc gcaaattttt
    59101 aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg atgaaccata ttttgtaggc
    59161 aaagacgttg cgagtgtttt gggttattca aacactaaag atgctttgtc gcggcatgta
    59221 gatttggaag ataagatggg gtcgcgaatc acgacctcag gtcaatcaag agagatgaca
    59281 atcatcaacg aatctggttt gtacagttta atcttaaaat caaaacttcc ctctgccaaa
    59341 aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa ttagaaaaca tggaggttat
    59401 ctaactccag aaaaagtaga agaagctttg cttaatccag atacaatcat tcaattagca
    59461 actcaactaa aagaagaaag aactggaaga ttaatcgcag aacaaaagat tgcagagtac
    59521 gaacctaaaa tctcctattt agatagcata ttatcttcta cagattcagt aacaattagt
    59581 cagattgcag cagattatgg gatgtctcca caacagatga ataaattact tcataaacta
    59641 ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca aaaaacacat gaaccaagga
    59701 tacacaaaat ctcatacaac tgagatcccg aaagccgatg gtggcactaa aattgtaatg
    59761 aataccaaat ggacacagaa agggcgtcta tttatctacg aattactaaa aaaagaagga
    59821 tattaccctc aaatggattt agaggaaatt ggttagaaag gagttttagt atgactgaca
    59881 ttgcagaaat cactcaacga gatagagaaa aaatcaaaga atatgtcgaa agttcaaagt
    59941 tcttaactta caccatgctc gctgaaagat tcggcattag caaaagctac ttatctttaa
    60001 ttttaaacgg taaaaagact tctgcagaag caaatagaat catagattcg attattacta
    60061 tgtacgaatt gtaaagggag gaacagctaa tgcaatatct agaagcgaaa atcccagttc
    60121 cagaaggcta tgtaattatc tcccaagtgg attatgagga gttaaaaaaa gctgatgata
    60181 ctggtagatg gatgacgttg ccagaagtgc tagaacggat taacagaaaa tatgattggt
    60241 ttacttctag agttttaaag aacccaagat atagaaatat tatcgatatc gaaaaaaaca
    60301 aaaatggatt tgtctattat ccagttgaag gaagagacac atatctattt ttaagaagta
    60361 aaacacttga atttttagaa acaaattttt cggaaatctt aaggaggcaa gcggatggga
    60421 aaatttaata gagcgctagt attcagcgca ccgctaatca tctacgcttt aggactttgg
    60481 ggaagcagac aagcgttgat aggaacgatt gtttacatgg tttggatttt tatggggctg
    60541 gatgaagctg agtacaaaac aaaaaagcca gtcgggaggg actgactaat gaaaaaaagt
    60601 ttaatgacta taaacgaaaa acaattgaaa gaaaaattta atgatctcat caaagaattt
    60661 gtaaaagaaa ctggagaatt tcctaatcaa attcatctag ttgcggaggg gcatagccgg
    60721 tatcaagctg tgaaatttga gatgaagaaa caaatctatt gaatttagca gtacctctac
    60781 ttgtaagaac atttattgta atgttttttt cgactaaaga ttcatcgtat ctaaaaacca
    60841 acgtagtaat ttttgactca tacggtggaa tggtaatagg taatgacaac ggaggaaatt
    60901 ttgatagatc agcacctttt tttaaagatt taccagaata cgcacgtata ccatgtggaa
    60961 gttcgtcata cattgatttt accgttacag aataattatc accaatcatt gtaaaagcat
    61021 ttaaaggatc aggaattcca gaaatcgaaa actcaataat tgtaacaggt aaagaactgt
    61081 tatttgttaa caatacagaa tctataatcc tgaatttatt ttgatggtaa acatctggtt
    61141 tggaatcact ttctctatct atcaaccatt cttcgcattc agcgaagaaa tccaatttta
    61201 aattaaattt tttatttttt ctatctgtat aggagatata aaaagcaaat gttgaaaata
    61261 ctagagacgt aataggcaaa ataaaatctt taatggtttt aacaatgtta ttccagtcaa
    61321 attgtttaat aaagtcaata agactcaatt tataccacca ccatttttta tctacattat
    61381 atcaaagagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt
    61441 gactacgagc atacaagtgg cagaaagctt tgagaaaaaa catcaacatg ttttaagaga
    61501 tattgatgca ctaaaaaaag atgtgtccaa ttttggacag atgttttctg aaggtaatga
    61561 gccagattcc tacggaagaa atcgacgagt tttcttcatt agcagagatg gatttttctt
    61621 gttagctatg ggatttacag gaaagaaagc tatttacttc aaacaaaaat acattgaagc
    61681 attcaacgaa atggaagatg ttattcgcaa gaatactgtt cctcaaacaa ttgaagacat
    61741 gatgatctac caactagaag aaatgaaaga tgttaaaaaa gatgtctcca tgcttaaaga
    61801 tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa atacgaaagt
    61861 tatggaagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca aaaaagtatt
    61921 ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg gcgagttacc
    61981 tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttaccag aaactgcgat
    62041 tcgcatggaa atcgatcaac tgaacagaca acaaagactt tttggtgatg aaaatgaata
    62101 gagctgaagc gctaagaatc gggacggtaa ttgctaatcg ctggtggaga cacaataaac
    62161 caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa ataaaaaagt
    62221 gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat tttagcatat
    62281 ggaaaaagaa ctttccactc tagatcaata tttgactgat cctagttggg gcaaatcgaa
    62341 tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacggatg aagaactagc
    62401 atgtgatcaa gacgatttgg gaaattttgt gagtatttgg gatcatgtct atcttatcca
    62461 tttatcgagg aagtccaaaa aaacctgaat atatctatgt catcgaagat ggcttgattg
    62521 atgcgctaga agagtatgac agagataact tgattgatat ctcttattac ggatcaggta
    62581 agaaatacat tgctgaaatg gaggcagaat ttgatgagtg aaaacaaagg gacaacgact
    62641 tttgaaaaac tttttagtcg caagttaaat aaaatactca agaaaaaagg aaattttgat
    62701 tatttatctt gggctcacgc atgggagatt atgaaaaaga atgatccaca ggcaacggta
    62761 actattaatg agtataaaca ctacagagtt gtttctggaa ctcatcaaga ctttcttgtt
    62821 gaggaatata aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg
    62881 gttaaaggac acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt
    62941 gttaaaccaa atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg
    63001 gctctacacg gactgggatt atatgtattt caaggggaag atattccaac accacctaga
    63061 atcgatacga agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg
    63121 ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg
    63181 ctcttagctg ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg
    63241 gagcgagcaa tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac
    63301 cattaatcga ttcatattca gcggttctga aaaagttcaa aggaaaagac ataggtgcaa
    63361 ccatcaatga ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg
    63421 atcgagttat tgaaattcgt tttattgatc ctagacgttt cacagtacag caacgaaact
    63481 tcatctatgc actcataggc gatattttca tcgatacagg catgccaacg gacttctgga
    63541 aggaattctt ttacttccgc tttgaaggtg tcacagggcg caaaataagc ctcaaagatg
    63601 aatcgaatac gactgtgagt gatgctaacg tcttagcaaa tatcatctta gatttcatct
    63661 ttgaacatca tattcctttc aaagaaggct atgagatttt accagcgaat caagaatatt
    63721 acttctacaa atgcatcaca aaaagagtct gttgcatctg tggcaaaaca ggagctgata
    63781 tcgatcactt tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat cattcagagt
    63841 acacatttgc agcactctgc agaatccatc acacggagaa acacaagata ggtgtgatca
    63901 atttcaaaaa taaatatcaa atcaaaggga tcaagttaaa ccaggaaaca attaagaaac
    63961 ttagaatagg aggataaaaa atattggctg acaacaaacg atactactat ttaaaactaa
    64021 aagagaactt tttcgatagc gacgagatgg ttcttttaga aagtatgcca gatggataca
    64081 tttattctaa tattcttctc aaactttatt taagaagtct gaaacatgaa ggcaaactaa
    64141 tgtttaatga caggattcca ttcaattcta caatgcttgc gactattaca agacactctg
    64201 tgggagttgt agaaaaagca gtacaaatat ttcgtgattt acaacttatt gaggtattag
    64261 ataacggagc aatttatatg tctgatatac aaagttttat tgggaaatct tcaactgaag
    64321 ctgataggaa gagaaaatac agaaaagaaa ttgaggaagc aaaacagaat ttaataacaa
    64381 gtggacaaat gtcggacaaa tgtccggaca aaactacacc agagttagag atagagttag
    64441 agaaagatat agagttagag aaagatatag agaatgtaac gccttcgaaa aaatcgaagg
    64501 ctaagcccgt ccgtcataaa tacggagagt ataaaaatgt tcttttgtca gatgagcaga
    64561 tggagaaact caaaatagaa ttccctaatg actaccaaga gcgaatagaa cggctatctg
    64621 agtattgtga atcatctggt aagacttata aaaactattt ggcaactatt cgaagctggg
    64681 caagaaaaga aaaaagtgaa tctaagaacg caagcagtgg atacaagcgc acaggaagac
    64741 gagagaagct tcctgagtgg gcaatcgacc aagaagccta tctcaagaaa aaagcgctag
    64801 aacgagctaa tagacaatca aaagcaccat tctaagaggt gggaaattga aaatcgatta
    64861 tctagaacta attaatgaaa tagcaaagta taaaactggt gaggaaatag aaatcctgag
    64921 agacgtatat gatcaactcg aagaagctgg aatcgaagga attaagaatg atcgttcgag
    64981 ttggagtaag ctcagatact atttcgcact ttatatcgat acaacacaat taagaaattt
    65041 agcatatacc aaattactat ttgttgattg tgttaaaggc ttgcaaaaac atcttaatga
    65101 acttgagcag gtgtaatcag atggacctaa agacatttac agcacagatt gaactaatgc
    65161 atcaagaagc tttaagacaa agtgtatcgt acgaagacaa gtggctcaac acgttccacg
    65221 gtggacgtga gagcgcactt gatcaagtac tcaaattatt gaaaggggaa cgtcgggatg
    65281 gataagaaag cggcttggcg aaaattaatg ttgctgattc aagatgagaa ctggcaagaa
    65341 gatgaagcag tggttgctga agttcagcgt ctagaaaaga ttgctaacgg acgtatacga
    65401 aaaaagcctg ataagcggaa acttcgcaaa ggaaaaatca tcgttgtttt gtatgaaggt
    65461 aacattttga tgcaaggaac ggcaagagag ttgtctgcag aaactgaata tacgagcgga
    65521 actattcgga cgtacgcgtg gcggaatcac gttgataaaa aaggacacga atataagtat
    65581 ttggagggca gcaaatgata ccgaagttta gagcgtggta cacaccattt aaaggtaaaa
    65641 caattggaca agaaatgaaa tatgggcaag caggaaggtt gatcactcat gctgaaatgg
    65701 ctccagataa atatgtgctt atgcaatcca caggactgaa agacaagaac ggtgtgggga
    65761 tatttgaagg ggatgtagtt cttgtcaatg taagtaatgg ttttgatcat ttagttaatg
    65821 aaaaaacggt tgtacaagaa tccgagtttc attctggatt agtttgtaaa tcattaatta
    65881 gtggaatgga atatagagta ttcaaacaca aaaagatgga ttatgaatat gaagtcatcg
    65941 gaaatattta cgagaattca gagttattgg aggaacagcg atgaataaac aggaaacaat
    66001 caaaaagtta gaaagtatta aagcgatagg aaatgatgca atagctgctt gctataacga
    66061 gagtatgaat gcaggtatta cgttaatgaa aaaaatagac gaaccgcaga aacacgttat
    66121 gccgaagttt tttgacaaat gggcaaaaca agtcctggaa aaacgtgaca agttttatgc
    66181 catttctctt attgcacgtg caggttgggg atatggtgtt gattttgaac ttagcgagaa
    66241 cggattatca tcagaaaaca aagagctgtt gtactggctt gttgataaat gcagcgacaa
    66301 ttatcctaat aaaaagaaag caatagaagc tttgttatac ggctacgagg tcgagaaaga
    66361 accgttgtat cacgtgaagc taaaaatacc aggcgtaaca tactacctta tccaaacttt
    66421 tttaatgcca ggagatactc atatgtgttt ctcagcggct acagaacgtt atggggctaa
    66481 atggaaaaat acttttacgg aatcagaaat aaaagcaatt gatgaaagat actggccatt
    66541 tgctgtgcca gtggaagagg aggcagaagg atgaaaaaat tgcaccaact catatctgag
    66601 aaagaaagcg aactccaaaa tttagaagat agtttgggat tggggtttcc aattgttgag
    66661 caagctaaaa tgactcaaat cagtcatcta cgacttgaat tggaagattt aagacaaata
    66721 gaaaaatcaa ttcaactcaa cgacaaccag caaatcgtgt ttgagtggtt gaaactaact
    66781 gcgccaacag gaaagccaat gcaagttgta ttttggatga tgaataatgc agcatggggg
    66841 catttagacg aattaaggga tcctttgatg gaattaacag acaaagagca attcgaagtc
    66901 ctagcagcat tcgctcaatg gggattagaa caggaggaag cggaatgaac aacagacatc
    66961 gcagaataac gaaactaaga aaacaggaac tgaatgtact aaaggcaaag ttcgaaaaag
    67021 aatacggagt ttctgcagaa gaagcatata aagtggcaag tcagtgtgtt gctgatgcga
    67081 gtaatgctat tcgtaagttt ggaatttcga tattaagtga tgatcgtaaa tgggaggata
    67141 tagaatgaag gtttatgtag taaggttcgg gaataaattt tatagaagtg atgaacgctc
    67201 tataggcaat aatgtgttat tccttgtaga ttttcttcaa aacgcaagat ggtttgataa
    67261 tctcgaagaa gctaaccaag tttcacgacg acttggtgga ttaacgcaag tatatgaact
    67321 ggtcactatc gatcatgagg gggtagaaag atgaaactaa aagacggatt ttacgctagt
    67381 agtcatggta tcggcggttt aatgctaggt atgccgacaa agaaccctaa aacacgtaag
    67441 aaaccaaaat tcaaagtcgg tgacatggtt cgctgcgaag cagaggagtt catctatccg
    67501 tttcgtggat atgtagagca tgtttataat cactcagcga tcattcgcat tgaaaacacg
    67561 atggaatgtg acaagcggtt agcgaaaagc aaagagaatt tagcagtggt gagattggtg
    67621 gatatggaag ttataaaatc ttaattttgc ttttttacaa ctatcctaca acaatgagcg
    67681 ttattccaca actggaagga gaagaggcaa ttgtgaacat tttagagaat atagatatta
    67741 agcaaacaag gaaaaatgct agacgattac taaaaagata cagaaattta gaacgtttgg
    67801 tcggaccagt gaaaatagac ttttccgtga tgactgttac caaaaatttg aaattcacaa
    67861 ttgacagtca aaacgaagaa atcattgaag cgataagtac tcgagattcg gttatcgaag
    67921 cattaacacg gctaagtaga atccatttcc aggtacttta ttatagttat tgttttccta
    67981 ataagatgtc aatgtatcaa ataggagaaa aattaggata ttccgataga actatcgaga
    68041 gaatgaaggc agtagctttg gttgaatttg ctgaggcgta taaatcagga gaactcattt
    68101 cacgtacaaa ataaaaaaag tcggattcct ccgactatga ataatatttc cgacataagt
    68161 attatatcat aattggggga atcagaggat ggtacttttc gatgtaaaga aatatgagac
    68221 accaagcgcg aaagatgttg atatggaacg cacaaaacat aatgtcgctg tgtt
//
LOCUS       DACDLI010000006        66108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDLI010000006 DACDLI010000000
VERSION     DACDLI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.006"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(119..418)
                     /locus_tag="IV353_000423"
     CDS             complement(119..418)
                     /locus_tag="IV353_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9008700.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            769..1401
                     /locus_tag="IV353_000424"
     CDS             769..1401
                     /locus_tag="IV353_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9008701.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1524..2162
                     /locus_tag="IV353_000425"
     CDS             1524..2162
                     /locus_tag="IV353_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9008702.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2281..2353)
                     /locus_tag="IV353_000426"
     tRNA            complement(2281..2353)
                     /locus_tag="IV353_000426"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2318..2320),aa:Thr,seq:cgt)
     gene            2474..2716
                     /locus_tag="IV353_000427"
     CDS             2474..2716
                     /locus_tag="IV353_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008703.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2855..3484)
                     /gene="upp"
                     /locus_tag="IV353_000428"
     CDS             complement(2855..3484)
                     /gene="upp"
                     /locus_tag="IV353_000428"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9008704.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3618..4565)
                     /locus_tag="IV353_000429"
     CDS             complement(3618..4565)
                     /locus_tag="IV353_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9008705.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4718..5962)
                     /locus_tag="IV353_000430"
     CDS             complement(4718..5962)
                     /locus_tag="IV353_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9008706.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(6137..7162)
                     /locus_tag="IV353_000431"
     CDS             complement(6137..7162)
                     /locus_tag="IV353_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9008707.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(7184..7363)
                     /locus_tag="IV353_000432"
     CDS             complement(7184..7363)
                     /locus_tag="IV353_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008708.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(7356..8195)
                     /gene="prmC"
                     /locus_tag="IV353_000433"
     CDS             complement(7356..8195)
                     /gene="prmC"
                     /locus_tag="IV353_000433"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9008709.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(8188..9261)
                     /gene="prfA"
                     /locus_tag="IV353_000434"
     CDS             complement(8188..9261)
                     /gene="prfA"
                     /locus_tag="IV353_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9008710.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(9264..9848)
                     /locus_tag="IV353_000435"
     CDS             complement(9264..9848)
                     /locus_tag="IV353_000435"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9008711.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9954..10423)
                     /locus_tag="IV353_000436"
                     /pseudo
     CDS             complement(9954..10423)
                     /locus_tag="IV353_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            10529..11131
                     /locus_tag="IV353_000437"
     CDS             10529..11131
                     /locus_tag="IV353_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9008712.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(11235..11804)
                     /gene="lepB"
                     /locus_tag="IV353_000438"
     CDS             complement(11235..11804)
                     /gene="lepB"
                     /locus_tag="IV353_000438"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9008713.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(12034..12801)
                     /locus_tag="IV353_000439"
     CDS             complement(12034..12801)
                     /locus_tag="IV353_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008714.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12798..13277)
                     /locus_tag="IV353_000440"
     CDS             complement(12798..13277)
                     /locus_tag="IV353_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9008715.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(13402..14139)
                     /locus_tag="IV353_000441"
     CDS             complement(13402..14139)
                     /locus_tag="IV353_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9008716.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            14300..15700
                     /locus_tag="IV353_000442"
     CDS             14300..15700
                     /locus_tag="IV353_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9008717.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15923..17275
                     /locus_tag="IV353_000443"
     CDS             15923..17275
                     /locus_tag="IV353_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9008718.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            17268..17966
                     /locus_tag="IV353_000444"
     CDS             17268..17966
                     /locus_tag="IV353_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9008719.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(18028..19179)
                     /locus_tag="IV353_000445"
     CDS             complement(18028..19179)
                     /locus_tag="IV353_000445"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9008720.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(19192..19629)
                     /locus_tag="IV353_000446"
     CDS             complement(19192..19629)
                     /locus_tag="IV353_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9008721.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19644..21410)
                     /locus_tag="IV353_000447"
     CDS             complement(19644..21410)
                     /locus_tag="IV353_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9008722.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21674..22351
                     /locus_tag="IV353_000448"
     CDS             21674..22351
                     /locus_tag="IV353_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008723.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            22354..23481
                     /locus_tag="IV353_000449"
     CDS             22354..23481
                     /locus_tag="IV353_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9008724.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23681..24121)
                     /locus_tag="IV353_000450"
     CDS             complement(23681..24121)
                     /locus_tag="IV353_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9008725.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(24132..26165)
                     /locus_tag="IV353_000451"
     CDS             complement(24132..26165)
                     /locus_tag="IV353_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9008726.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(26167..27870)
                     /locus_tag="IV353_000452"
     CDS             complement(26167..27870)
                     /locus_tag="IV353_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9008727.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27964..28914)
                     /gene="manA"
                     /locus_tag="IV353_000453"
     CDS             complement(27964..28914)
                     /gene="manA"
                     /locus_tag="IV353_000453"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9008728.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28953..29978)
                     /locus_tag="IV353_000454"
     CDS             complement(28953..29978)
                     /locus_tag="IV353_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9008729.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(30187..31056)
                     /locus_tag="IV353_000455"
     CDS             complement(30187..31056)
                     /locus_tag="IV353_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9008730.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31760..33652)
                     /locus_tag="IV353_000456"
     CDS             complement(31760..33652)
                     /locus_tag="IV353_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008731.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33649..35376)
                     /locus_tag="IV353_000457"
     CDS             complement(33649..35376)
                     /locus_tag="IV353_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008732.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(35392..36042)
                     /locus_tag="IV353_000458"
     CDS             complement(35392..36042)
                     /locus_tag="IV353_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9008733.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            36246..36542
                     /locus_tag="IV353_000459"
     CDS             36246..36542
                     /locus_tag="IV353_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9008734.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36734..37354
                     /locus_tag="IV353_000460"
     CDS             36734..37354
                     /locus_tag="IV353_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9008735.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(37446..38612)
                     /locus_tag="IV353_000461"
     CDS             complement(37446..38612)
                     /locus_tag="IV353_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9008736.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38624..38977)
                     /locus_tag="IV353_000462"
     CDS             complement(38624..38977)
                     /locus_tag="IV353_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9008737.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            39105..40127
                     /locus_tag="IV353_000463"
     CDS             39105..40127
                     /locus_tag="IV353_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9008738.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(40566..41000)
                     /locus_tag="IV353_000464"
     CDS             complement(40566..41000)
                     /locus_tag="IV353_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008739.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(41110..42969)
                     /locus_tag="IV353_000465"
     CDS             complement(41110..42969)
                     /locus_tag="IV353_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9008740.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(43087..44246)
                     /locus_tag="IV353_000466"
                     /pseudo
     CDS             complement(43087..44246)
                     /locus_tag="IV353_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(44553..45977)
                     /gene="araA"
                     /locus_tag="IV353_000467"
     CDS             complement(44553..45977)
                     /gene="araA"
                     /locus_tag="IV353_000467"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9008741.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45993..46691)
                     /locus_tag="IV353_000468"
     CDS             complement(45993..46691)
                     /locus_tag="IV353_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9008742.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46648..48309)
                     /locus_tag="IV353_000469"
     CDS             complement(46648..48309)
                     /locus_tag="IV353_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9008743.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            48527..49927
                     /locus_tag="IV353_000470"
     CDS             48527..49927
                     /locus_tag="IV353_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9008744.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(50179..51258)
                     /locus_tag="IV353_000471"
     CDS             complement(50179..51258)
                     /locus_tag="IV353_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9008745.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(51344..52303)
                     /locus_tag="IV353_000472"
     CDS             complement(51344..52303)
                     /locus_tag="IV353_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9008746.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(52315..53838)
                     /locus_tag="IV353_000473"
     CDS             complement(52315..53838)
                     /locus_tag="IV353_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9008747.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53848..54411)
                     /locus_tag="IV353_000474"
     CDS             complement(53848..54411)
                     /locus_tag="IV353_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9008748.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(54417..55280)
                     /locus_tag="IV353_000475"
     CDS             complement(54417..55280)
                     /locus_tag="IV353_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9008749.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(55294..56190)
                     /locus_tag="IV353_000476"
     CDS             complement(55294..56190)
                     /locus_tag="IV353_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9008750.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(56365..57636)
                     /locus_tag="IV353_000477"
     CDS             complement(56365..57636)
                     /locus_tag="IV353_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9008751.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57626..59143)
                     /locus_tag="IV353_000478"
     CDS             complement(57626..59143)
                     /locus_tag="IV353_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9008752.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            59341..60231
                     /locus_tag="IV353_000479"
     CDS             59341..60231
                     /locus_tag="IV353_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9008753.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(60304..61128)
                     /gene="nadE"
                     /locus_tag="IV353_000480"
     CDS             complement(60304..61128)
                     /gene="nadE"
                     /locus_tag="IV353_000480"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9008754.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(61140..62612)
                     /locus_tag="IV353_000481"
     CDS             complement(61140..62612)
                     /locus_tag="IV353_000481"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9008755.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(63249..63611)
                     /locus_tag="IV353_000482"
     CDS             complement(63249..63611)
                     /locus_tag="IV353_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9008756.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63834..64586
                     /locus_tag="IV353_000483"
     CDS             63834..64586
                     /locus_tag="IV353_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9008757.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64726..65649)
                     /locus_tag="IV353_000484"
     CDS             complement(64726..65649)
                     /locus_tag="IV353_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9008758.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65664..65936)
                     /locus_tag="IV353_000485"
     CDS             complement(65664..65936)
                     /locus_tag="IV353_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008759.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        19128 a        13539 c        11479 g        21962 t
ORIGIN      
        1 gaaacatctg tttctttatt tagaataaaa aaatacagac tctgttcaaa taaatgaacc
       61 tagcctgcat tatgagattg actatacttt ttaattcatt gagatgaata actcgttttt
      121 ataaatgccc aaaagtaatc ggtgccgaag ccattcctcg acctaattct ttttcaaatt
      181 ttcctattgt tgatatctcc atataaaacg gtgatactgg tttagacaat tctcccctga
      241 ttgctaacgg acgtaaacta cgttccaaat tatttatgac tttaggataa tactgacctt
      301 tttctaagcc tgtctttgcg tctatcaaaa gtatacgttc cttttctttg atctctggat
      361 ttaaaacgag atcatataat tttagtacca tgttttctat ttcttcactt ttagacatca
      421 cgatttcctc ccaaaaatat atttcttccc actacattct ataaacaaag tatacgctac
      481 atctagattt ttttaaatca ttactggatt gttaaaacct ttcaatacac tgttatggca
      541 acgattttta ttttaaaaac attctaaatt aaatcagtaa aaatttttat tctaagattt
      601 ttattagaat tacgcctaat ttgtgtctct cagggcaatt ctaagaacgt ttcaagagat
      661 agcgacacta tcgttatcga aaaaggcgaa aacctatctc attttctgct aatgtaagtt
      721 ggtttagctt ttgttaatat gactttatga aaatggaggt aattaaatat gatttttcct
      781 aaagttaatg aagcaagctt gaataaagga tccttttttc atatctatga aaacgaagat
      841 tttgaactgt ataatatcct ttcacaaaaa gaggtcatcg aaaaccaaat aatctgccat
      901 ccaattattt acaaatctat tttcaagtat gcattttttg cttccaatga ttttcaaaga
      961 atggagatta ccgactgtat attcgaaaac tgtgattttt caggctgtat attgacaaaa
     1021 agcatgttgc atcgcgtgaa atttgtcaat tgtaaattaa caggtactaa attgatggaa
     1081 agttatattg ggaacacgtt attcgagaat tgtaaaatgg actatgtaaa tttatctggt
     1141 tcaaacataa aagaaagtac ctttgagcac tctgtcttat caagcgcaga ttttgtagac
     1201 tgttccctca ctaaaataat gttttctact aatgatttag aaaatgctag cttttttgat
     1261 actgatttaa aaaatatcga tttaagtagc aactcctttg aaatgattga agtacgctcg
     1321 gaacatctaa aaaattgtac gcttaatcag ttgcaagcac ttggttttgc tagaaaattt
     1381 ttacaaataa aagtagtata atcaaaatga atatataaga taaattttta gcaatacaaa
     1441 tatccataaa aaaacattgc ctattttaac tcttcatgta tactgactct atcttcaaaa
     1501 actgaatagt aggagttatt atcatgaata acattttagg aaaaaaaata gaacttaaaa
     1561 aagggatatt gggcactatt gtcgttgtac tcggtttgtt agggttgcta tttgcgccat
     1621 ccattattag cattttttct ttaactatca ctggatttat agcatttaca cttgtttttg
     1681 gtaaaaaaga tacgaaaaaa atgttctcga aacctgtcgc tcctgtcaaa aatatagcca
     1741 aatatttttt tatcaatgta gtaatcagtg ccgcagtctc ggtatttttg caacaaattt
     1801 tgaaatgggg tttaacaggc aatccaataa atgaggcttt cagcccgtta ttatttatta
     1861 ttttgcctat catgatactt ggagaagaat tgttctcagt gtattttctc gctatttttt
     1921 ctagtaagtt cagcattcct gtcgctagtt tcttgtcagc aatcatcttt ggatttatcc
     1981 attacagtac ttatgataat ggaaatattt tacacactgt agcacacatc cttttgatac
     2041 aaggggtggc aagattgtta ttcaaccaag ctgccattaa aagcaactcg atcattacaa
     2101 gttgggcagt tcatgtgata tttgatttca cagctatatt gctagtcgtt ttattctcct
     2161 aaatttttat agaaataata catgctctat tgctaagaaa gcatacttta tttcctttaa
     2221 acaaaaagac agatgtatca atggattaaa aatccttgat atatctgtct tctcttttaa
     2281 tgccagctat agggatcgaa cctacgacct acgcgttacg agtgcgttgc tctaccaact
     2341 gagctaagct ggccttatcc aaaagaaatt atatactcta tttggctgat tgtaaagagt
     2401 ccgatggaaa attagtttaa tttcatgata tttttcataa aacgtggtat aattgatgtg
     2461 aaaggcaggt tttatgaaaa tggcaacttt tggaaaattt gatacaacta tcgtaccatc
     2521 ggaaattggt gaaaaattca agatcaatac tcacgtaatg ttcacaattc acggcaagtc
     2581 gcaaactgga acgatttcaa agcaattgaa aaattccgct gttgttgaga ttgatgaaac
     2641 tgcagaaaat gcgaacttga tgacgcaaag taagggtgta gtaatcgtta actacaaaga
     2701 actaaaaaag atttgaattt gaccatctct gcatttttgg gatggttttt ttatatgcaa
     2761 acgaaagctg tgaaaaagct gttctgcatc aaacgttaaa aatgaccaaa aaaggatgtg
     2821 acaaaagttg tgtcacaccc tttttcgtat ttacttattt tgtaccaaac aaacggtcac
     2881 ccgcatcccc tagtccagga acgatataac catgttcatt caatttttca tctagagaag
     2941 ctgtatagat gtcaatatca ggatgcgctt cttgcaaagc ttttactccc tcaggtgctg
     3001 ctaccaaaca aacaaattta atgttcgacg cgccacgttc tttcaatgca tcgattgcca
     3061 tgattgctga tccgcctgtt gccaacattg gatctactac aaacaactga cgtgcatcaa
     3121 tgtcttctgg aagtttgacg aaatattcat gtggttccaa tgtttcttca tctcgataaa
     3181 gaccgatatg tcccacttta gcagctggaa tcaattccaa gataccgtca accattccaa
     3241 tacctgcgcg caagattgga ataatcgcta cttttttacc agacaacgtt ttttgtgttg
     3301 tttcagtgat cggtgtttcg atcacgatgt cttccaatgg catatcccgt gatacttcat
     3361 atgccatcaa catagcgatt tcatcgacta cttcacgaaa aacctttgtt ccgcagtttt
     3421 tgtcccgaat aatagttagt ttgtgttgaa tcaatggatg atcgatcact tgaaatttgc
     3481 ccatttttta agctctcctt tgttctgtcg tttcttttta attgtaaaac aaaatcaagt
     3541 gattgactag tcaaactcgc catttttcct aagaaaatcc gtttttgaaa agaagttttc
     3601 ctgcggacaa gcaaagctta ttcttttttc ttgatccaag aagtagataa tgccattgct
     3661 gcacgcgttg cctttgtctg atccatagca tttaccatga caaaatcatg gatgattcct
     3721 tggaagcgta cttgtgtaac aggaacccct gcatcacgaa gtttgcgcgc ataagcctca
     3781 ccttcatctc ttaatacatc cgcttctccc gttaaaatca ttgctgaagg aagatcattt
     3841 agttcattta tatttgctct caaaggcgaa gctgtgattt cagcacgctc ttcttcattc
     3901 gtcgtatatt ggtcccaaaa ccacatcatt ccttctcttg tcaaaaaata gttttctccg
     3961 aattcatgat aggaatctgt atcaaaatca gcgtttgtga ctggataata cagcaattgc
     4021 ttggatattg ggaggccctt tcgttctttt gttaagatcg tcatgactgt agccatgtta
     4081 ccacctactg aatctcctgc aacgattaga ttctcgagat ccagattttt tgacttggct
     4141 aatttattca gctgttgcaa cacagcataa ttttgttcaa ttgctgttgg atattttgct
     4201 tctggtgatc gactatattc tggaaagaca acgacggaat tcgtacgaac cgcaagttct
     4261 ctgatcaatt tatcatgggt ttgagcactg ccaaaaaccc agcctgctcc atgaatataa
     4321 aaaataactg gtaatttatc catattccct tctggtcgta cgagacgaat attgagtttt
     4381 ccccattcgc cagtatccac catcaagtct tcaatatcga cggaatattt gttcacaggt
     4441 gattcttgta cttcttctag taaaactctt ccttcttccg gtgctagttc atagatacgc
     4501 ggatgaggcg catttgcatc actgaatgct actgcttcct tttctaacga gatacgatca
     4561 gtcatgactt ttcctccctg agatttagtg tgaagactaa aattacacct catatactag
     4621 agagcatcac cgatattgtc aaagaatccg ttttcccaaa aaataaacag aaaagcccaa
     4681 cattgaactt ttctgtttac taatggaatt taaattttta ttttttatgc aacggaaatt
     4741 tttcagttaa ttcgcgtaca cctgttttga cttcgtctag ttgagcgtta tcatcttttg
     4801 cttgtaatgc tttgacaact agttcagcca cttttacagc atcttcttct ttgaacccgc
     4861 gagtagtgat tgctggcgtg ccgattcgaa taccgcttgt tttgaatgga cttagtgttt
     4921 caaaaggaat cgagttttta tttacagtga tattcacact gtccaaaatg ctctctgctt
     4981 cttttccatt tatacctaac tcgcgaacat cgatcaacat cagatgatta tctgtcgccc
     5041 ctgaaatgac tctggttcca attgcttgat tgaaaacttt gaccattgct tttgcattgg
     5101 cgatgatttg ttcactatat tctttaaatg caggatctaa tgcttctttg aatgctactg
     5161 ctttacccgc aatcacatgc tctagagggc cgccttgaat accaggaaat accgcactat
     5221 ttattttttt tgctaatgct tcatcgtttg tcaaaatcat gccgcctcga gggccacgca
     5281 atgttttatg agtggtagtc gttgtgatat ctgcataagg aactggatta ggatgaagtc
     5341 ctgctgctac taatcctgcg atatgtgcca tatctaccat cagttttgcg cctacttcat
     5401 ctgcgatttc tctgaatttc gcaaagtcga tcgttcgtcc gtaagcactg gcacctgcta
     5461 cgatcaattt tggttgatgt tttctagcta aaatacgtac cacattataa tcaataactt
     5521 ctgttgtcgg atcaacaccg taagcaacaa agtgataggt tttgccactg aagttgactg
     5581 gtgatccatg tgttagatgt ccgcctgcgg aaagatccat tcctaagatc gtgtcccctg
     5641 gttctaccaa agcgagataa gcagccgtgt tggcttgaga gccggagtga ggctggacgt
     5701 tcgcaaattt cgctccgaat aattcttttg cacggtcaat cgctaaattt tcaacgatat
     5761 cgacaaattc acacccacca taatagcgat gccctggata accttctgcg tatttatttg
     5821 tcaagatact tccttgtgca gccatcactg cttcggagac aaagttttca gaggcaatca
     5881 gctccaaatt atgttcttgt ctttcttctt ctttggcgat cgcagcccaa agatcagggt
     5941 caaacgtttt gtaatctacc acaaacaaca gctccttttt attttcctac aattttattt
     6001 ttcccatcaa aaacaaattg ccaaatattc gtatctcttc tattacgatt agttacgctt
     6061 agtttttcgt tcactgatag aagatttact tgatagcatt gtatcatact aaaagaatgc
     6121 gacaataacc aattgatcat ttttcaaaat aattttgtcc tgctgctttt ttcaagcgat
     6181 tcatgtaagc attccccaat ccagtttctg gatacacttg tacaaaaatc aaatctaatc
     6241 caagtgttgg ttcatctaat cctcttagac ctgcaaacaa tcctttagcc gccgcttcta
     6301 ctgagttgtc gttatacata taaacagctg cagtatctgt ccgcacttga tccgcaattt
     6361 cggggcttgc tatgacacct gcacggatct tcttattttt tgcccattga actgcagttg
     6421 accaatcgcc atctctgacc atcagaacac gtgtatcggg tgagtagtgt ttgtatttca
     6481 tcccaggggc ttttggtgtt tcattttctt ttacaagatg ctgatcaata gcaacaggag
     6541 attcgatgac tgcttcgatt tgttctttcg ttactgcacc tggtcgtaaa atcataggca
     6601 tggctgttgg atcacttaaa tcaagaacag tggattctac accaattcgc gtagctccat
     6661 catcaataat cccggtaatt tttccttgta gatcatgata tacatgatct gcagtcgttg
     6721 gacttggttt tcccgacgta tttgcacttg gaccgacaag cggaacaccg gaaagttcga
     6781 ttacttctag tgtttttttg ttatccggta tacgaaatgc agctgtggat aatcctcccg
     6841 tcacaactga gggcaaagtg tcttttttga tcttaaatat caaagttaat ggtccaggcc
     6901 agaagttctt cacaatcttc tctgctgccg gatgaaagtt gtccacatac tcttttactt
     6961 gttcaaaaga ggcgacatgg acgattaatg gattatcttg cggtctccct tttacagaaa
     7021 acactttctt gacagcgttt ggtaaaagag cgttggcgcc caatccgtat actgtttcgg
     7081 taggaaaagc aacaagttct ccttctttca gtgcttcggc tgcctcttgc aattctgttt
     7141 catcatatcg tttcgtttcc acagtttatc cacaacccct ttttcataca ttcttatttt
     7201 gtctgagttt atcctcaagt tttccactgt ttgtggataa tctatttgtc aatagtggat
     7261 aaacttgttg ataaaccaaa aagtcgttta catccttgtc aaataagtgt ttttttataa
     7321 taaaaaaaca aataaaaaat ttttcatcca caacttcaac cataaacttg gatcattcgt
     7381 tcattgccaa aaagatcctt ttttatcgcc acttttttat ctggaaatgc ttgctgaaat
     7441 atttttttta ctgcttcacc ttggcgaaat ccgatttcta aaaaaatctt tccatttgat
     7501 tttagtacgc ttggtgcttc aactgctatt ttttcataaa tagctaatcc atcgttctca
     7561 gcaaaaaggg ccattttagg ttcgaatgac cggacacttt catccatcag ttcccattca
     7621 ttccgactga tgtacggcgg attagaaata atcacatcaa attgctgatc cattactggt
     7681 cctaatgtat ctccatgata aaaactgatt ttcgttcctt ctttctgagc attttctttc
     7741 gctacttcta atgcttcttc tgaaagatcc acagcagata tatgccaatt ttttcttgct
     7801 gctttcaaac taatagcaat tgctccagtc cctgtcccaa tatctatcac ttctaacggt
     7861 acaccaggcg tctcgtctaa acaccattcg actaactcct ctgtttctgg tcgagggatc
     7921 agtgttgctt cagttacctt taaccgatga tcataaaaat ctgcatatcc tagcaaatac
     7981 tgaggtggat gatctgcaag cagaagtcgc agatcggttt cgatcaaccg ctgttcttct
     8041 tctgaaatct cttcgttcat atgaaggagc caatcaagtt ttttccattg ttttcgttcc
     8101 aaaaataaaa attggatact atatgcttct ttcccatgat cctctaaaaa agaagaagcc
     8161 cgtgacagga cttctcgata ggtcatttta cccattttgc atagcttcca gttttgatgt
     8221 ttgatcatac atgatcaatg catcaacgat ctcatctaat ttccctgcta aaatctgatc
     8281 taatttttgg atagttaatc cgatacgatg atcagtcaca cgattttgcg ggaaattata
     8341 cgtccggatc cgttctgaac gatccccagt tccgactgct gattttcgat tggcatcata
     8401 ctcgctttgt gcttcttgct ggatcttatc gtacacacga gcacgcaaga ttttcattgc
     8461 tttttcacgg ttcttgattt gagaacgttc atcttgcata gcaacaacga tccctgttgg
     8521 taagtgagtc aaacgtacag cagatgctgt tttattgacg tgctgtccgc cagcacctga
     8581 agcatgatag atatctgtac ggatatcttt gtcttcaata tcgatctcta cttcttcagc
     8641 ttctggcatc acgacaactg ttgcggtgga agtatggata cgcccttgcg attcagttga
     8701 aggaacacgc tggacgcggt gcgcaccact ttcatatttt aatttcgaat atacgttgtc
     8761 tccggtgatc atcatgatca cttctttata tccaccgatt ccggtaacgt tagcttccat
     8821 cacttctgtt ttccagcctt gtgcttcagc atacttttgg tacatattga aaagatcgcc
     8881 agcgaataat gctgcttcgt ctccgcctgc tgctccacgg atttccatga tgatattttt
     8941 atcgtcattt ggatcttttg gtaaaagcaa gatcttgatt ttttcttcta agacttcttt
     9001 ttcttttttt aattcagata attcttcttt tgctagttca gccatttcat catccagttt
     9061 ttcaccaagc agttcttctg tatcggtgat tccttgtact acttctttat aacgacgata
     9121 aacttcgact gtttcgcgcg tgtttgcttc ttcttttgaa agctgcataa aacgctttgt
     9181 atcagaaatc acttctgggt cacttaaaag ttctccaagc tcttcataac ggtcttcaat
     9241 tgcttgtaat tgatcgtaca tgcttattct ccttctgtaa tcattgatgg gtgaaagtaa
     9301 tgttttcgac aaacggggta ataagcttca tttccgccaa tttgcacttg atcgccttca
     9361 taaacagggt ttccatcaat ataatgtaaa ttcatgattg cttttttgtg gcagaaccaa
     9421 cagattgttt tcatttcttc gattttgtct gcataaagca ataaatattt tgatccttca
     9481 aataactcgt ttcgaaaatc atttttcaaa ccaaatgcca taaccggaat atccaattcg
     9541 tccacaattc tcgtaaaagc aatgacatgt ttctttttta agaattgcgc ttcatcgatc
     9601 agcacacaat agggtttgct ttccattttc tgaattacgt caaatacgtc tgtttcttca
     9661 aatatcggca tcgcattacg tttcagtccg attcggcttg aaacaacgcc taaaccatct
     9721 cttgtatcaa taccgcttgt cattaagaca actggcttat tttgttcttc ataattatgt
     9781 gcgactttta ggatctcgat cgtcttgccg ctattcattg cgccatactt aaaaaatagt
     9841 tgtgccatgc tctttttcac cttccagttt gttcctatgg tattatactt gattttcagc
     9901 attttttaca gtgagaattt tcatcaagca cagattagta ttaaatctat tgctcacttt
     9961 tgttccaatt ttttgacaag aactaaacat gggttccatt catcccataa atttggaaaa
    10021 acttccaacc ttgaaaatcc taatgattcg tagaaacaaa tagtttgatc atatatagag
    10081 tagtgcccct cgtctacagt cttcacttgc aaatatttgt aatgttttgc tgcgtaacct
    10141 tctgcttctt taaaaagttt tgttcctaat ccttggtgat gatagtcttt ttttattccc
    10201 atacaataga atagatttcg gcagtatctt cacttgtctg tgaaagagtg ataaagccta
    10261 aatattcttc ttttcttttt tcacaccata aaggtagttt acttgattcc tcgatatatt
    10321 tttccgttga ttcaggaagc ccaaaccact caggcaagtc tagtaatatt tctcggacac
    10381 actttccttt ttcctctcta tcttttacaa acgatatcgg catattcttt ctagattctc
    10441 cctttttgaa ttgagtatag catgctttct ttcttttcac gtgctataat aagttaaaat
    10501 ttcaaagttt tacgtaagga gctatttgat gacaaaaaat atttttgatt ccatcgctaa
    10561 ccattatgat tcccctgacc gtcaagcatt agccaagatt attcgcgaag aactaactac
    10621 ttatctaccg cgcgacagcc accaaaaagt cctgctggat tacggaggcg gaactgggct
    10681 tgttagttta ccgcttgcgg aaagattcaa agaactgatc attgccgatg cttcagaaac
    10741 tatgctgaaa atggcagaag aaaagatcca agcagcagat ctgaaaaatg tccgaacgat
    10801 ccacgctgat gcttctgtcg aatttcctgc tgtccaagct aatctcatcc ttctatcact
    10861 tgtactttta cacattcctg atacggagaa tattcttaca aaactttatg aaattttagc
    10921 tcctggcggt caattgatca tcgtagactt tgacaagaat gaacaaatta gtcatccgaa
    10981 agtccataat ggctttagcc aagaagaatt gaataatcga ttggaaaaaa cgggctttgt
    11041 ctctgctact agtcatacct tccatcgtgg ggaaaagcta tttatgaaca aaaacgcttc
    11101 gcttttctta tccatcagcc aaaaagctta aaatgagatt gcaaaaaaag tgagcaactc
    11161 caagaaataa gaaggaattt tcggaaaaag aagctgtgac aaaaaacatg tcacagcttc
    11221 tttttatttt ttcatcacat gattttaatt tctgaaagag gccaataacg gaaaacaact
    11281 tttccttgga tcatatcttt gtcgactaac ccaaacattc ggctgtcttt tgaaatcaag
    11341 cggttatcgc ctagaacaag atactttcct tttggcacag tatattcaaa gtcactcgta
    11401 aaggtggatg aactttctgc ttgtgcttgg aatcctgagc tataagcata ttctccttgt
    11461 aacttatctt ctgaaaattc ttttttgaac tcatccagat aaggttcatc atactttttg
    11521 ccattaatgg tcaactggtc attttccatc ttgactgtat ctccaggcat accgatgatc
    11581 cgtttaacaa tcatccgttc ttcatccccc ggttcctttg tagtaataat atccattcgt
    11641 tcatagtttg agatcttaga tgaaatcaat cgttgtccat cacttaatgt agggtccatc
    11701 gaatgcccgt ttacagtaac tggtgtaaac acgtacactc tagctaataa aatagccgct
    11761 aatacaagga tcaaaatccc ccaatttttc aaaaattctt tcacagctta cctcctactt
    11821 gtattttcct cgttatttat tacgtttttt cacaatactt cgttgtactt tctttattct
    11881 acaatgttta taaatgaaag gaaagaataa gtgatgtgtt cgtcttatct ttctcgttat
    11941 ataaaaaagg aacgtgacaa aagacgtgtc acatccccct tattccgaat aaacgttttt
    12001 atcgcaacca ctgatacacg gcgtttcatc acattatgaa tgtcttcttc tttggctacg
    12061 actataaacc actggttctt cctgcgactg tgcctttttt gcaatcaatt tcagacgata
    12121 aggctcatga tagcgtaacc cgtctttgac aaaatcaccg ccgacgatga acaatccgac
    12181 aggttccaat gtgtcttctg ttgagtcaag tcgaatggtc cctacttttt ccatgtccga
    12241 gtagcttcgt ccaatattta gaacatactc tttctctcct gttttcttga catagtaata
    12301 aggatacaat gtatttctat cgaccagaaa tccatccatg cgaccgagtt catttgtttt
    12361 gatctcacca tttccaatca aataccgcat attctcggct aatttcaatt gataatcttc
    12421 gtcataagaa accatgacct cttgtgtatt cactttatcg atcgaatttc cattatctat
    12481 cgattcttct gtctggatgt tgaacgcttc atcaggtaaa tacttcacaa aaagctgagt
    12541 gacatcggct ttcgtgtttt tatctaaaaa gtaataaaat acatttaaaa cagtaaaata
    12601 aacaagcact agaaatgcaa gtccatataa gaaaaattga agatagtttt ggttttgcca
    12661 aagtccataa acaattctca ataaataaag cgtgggaatc acacttaaaa tcgtatatcc
    12721 acggttaaga tatttaggac tgatgtctaa atagccaagg aatttatgca ccccgttgat
    12781 aaggtctagt aaaaatgtca ttgagaatca cctgcttgct ggttatacgt ttgggtattt
    12841 cctgaatctt ggttttgctg tgctgtacgt gctgcatcat cattttgcgt gttatcatct
    12901 acagtcgtac tgcttggtat cgtttcggag ctagcacttg tatccggact tgtacttgga
    12961 ttggttgaat ccgatggagt gccatctggt accgtattac ttgtttcgct tgagctgtca
    13021 gttgtatatg tctcatcata cgacgaacta gttgttgtat cgtcccatgt acttgtcgta
    13081 gtggtttcat tcaaactttc cgttgttgtt ggttcagatg tcgagactga agtagttggt
    13141 gttgtttctt ctacagtact agaacttgaa cttggtgcac ggtagctgga actattcaca
    13201 atacctgtag tagtcgcgac aatcgtcgag attgttaata cagcgattgg cttcaaaata
    13261 ggtgctactt ttcgcatctt atctcttcct tcattgaaat ttattcttgt ttccttactt
    13321 tactacggac aaattataca gtctacctta tttctaccat aaattcttta tttttttctt
    13381 acgtttttcc gaaagatttc tttaattttc ctcaaaaact acggttgatg tttttaagta
    13441 agcttttgct tcccgattga tttctttcag atcgcatcct tgctgcttgg cagcgaacac
    13501 gcaaccacaa taacattggc gataaatatc atactctttg cacatttgca aagagcgttc
    13561 atagcctttg tttttcttga aatcacttgg caagtattgt gtcccgtaaa tcttttggat
    13621 atccattccg atctgattga tcaaagctgc atttttcttt ggcgagatgg tcagggcact
    13681 tccaaagtaa tcgtatccta gttcttgcgc tttttgcgca acaagatcta atcgcatatt
    13741 gaagcatgca ctgcagcgag caccaccttc tttttcgttt tcaagatggt ttgttaaaac
    13801 cattttggtg aattcatttg gacggtattc atcaacgatt agcgcaacat gatggtcagt
    13861 tttctcatta aattggtcta caaatcgttt ttgttcatgt aatcttcgca aatactcact
    13921 tttaggatga atattcgaat ttgaaaaaaa taacgtgatc tctgcatatt gagacatgaa
    13981 ttccaatgtg tgcgtactgc aaggagcaca gcatgtatgt attaatatct taggctttac
    14041 ttcttctttc tcccattgca tgatcatttt tttcagaact gtatcataat ttattttttg
    14101 atttttcatt ttttctacga tttgttgagc attaatcaat ttgttattcc tcctccttct
    14161 tttttctagg attttcctta ttataagcca tttctatttt aatcgaaagc tgtattgtta
    14221 tctgtttgat aatttctttg agtattccta attccccttg tatactggta gaaacgacta
    14281 ttgtcaggag gatagtactg tgcatgttga tttattcttt cgaaggctgc agcggcgagg
    14341 acagaagttt gctgccaatc atttctcatc gatccaaatc atcgtttttt attatatatt
    14401 gatgacggtc ttatcactcg ttctatttta tatgccgatt tttcgcgaac cagatagcca
    14461 tgtttctttt gtggacatgc tgttcatggc aatcagtaca gtgagtgtga ctggactatc
    14521 gacttttgat atcaattctg tgttcaatga taacggtatt atactgctgg aaatattatt
    14581 tcaagttggt ggtttgggga tcatgatgat ttctactgct tttgtcattt tttctaaacg
    14641 acggatcaca ctgaaacagc gacagctgat catgacggat atgaatcaac cgcgtttgtc
    14701 tggtatcgtc cgactgattc ggatcacttt tgctatttta atctggtttc aattactttt
    14761 tgggacattc ttttctattt atttttatta cagaggctat tttgatcgtt ggcgtgatgc
    14821 cgttttttac ggtttttatc aggcgatatc ggctgtcacg aactctgggt ttgacgtcac
    14881 tggggattcc atcaagccct ttgcacatga ctattttttc ttgatcctga tcatgttttt
    14941 gatttttgtc ggaggaattg gttttcctgt attgatggag tgccgggaat ggctattata
    15001 caaacgttcc aatgcgaagc ttccgtttcg tttttccttg tttaccaaat tagctgtgtt
    15061 agcttttgtt atcttgtttg tttcgggaac tgtcttgatt tatctattgg aaaaggacca
    15121 tttattccaa gattccaata tgagtgtcaa atggatcaat tcgatgtttt attcgattac
    15181 gactcgtaat gctggtctgc agattcacga tttgggtgat tttcaaatca caacactgat
    15241 cattttttct ttactgatgt ttatcggatg cagtcctagt tctgtaggcg gcgggattcg
    15301 aacaactact gtagcaatca tcggtttgta tctatattcc ttcctcaaaa gtgaagataa
    15361 catcaatatt tttggacgga gaatcgatca agatgatgtg cgaaaatcag tcgttgtctt
    15421 tatgctttct cttggtatgt gtttcttttg tatcgttttt ttatctgcta cagaagaaca
    15481 aacgctgatc tcgatcatca tcgaagtaac ttctgcgttt ggaacaactg gtttatcttt
    15541 agggattacc ggagacttgt ctgttgtggg caaaatcacg attgctacac tgatgtttat
    15601 tgggcggatc ggaatgctat atacattgat gttattcgtg ccaaaagaaa cacgagatct
    15661 aggctatgaa tatccaagcg agaaaattat tatcggttaa aatgggaata taggtaagag
    15721 ggaataataa aatcacagca gcttgaaaca atcattttgt ttcaagctgt ttttttgtta
    15781 aatcatgcga ttttgtccga tttcatgcac tttcagccgt aaccttttct taagaataag
    15841 aaaagcaatt taaccttttt ttcaacatat ggtatgatta gttgaattta ttcgtcagca
    15901 aatcgttttt ggaggttaca taatgggaat acgcagtcat tttgcgattg cagttggaaa
    15961 aacgtcgcaa tgggcattaa aaactttttt taaaggcgga tcaagtttac caggtaaact
    16021 agcgctgaaa gtcgatccac atatactaga tagtttggca aaagactacc aagtcgtcgt
    16081 tgtcaccgga acaaacggta aaacattgac taccgcttta acagtcaata ttttgcgcca
    16141 gcaattcgat gaagttctaa ccaaccctac tggtgcgaat atggtacagg gaattgtatc
    16201 gactttcttg caggcaaaac caaaaaaagg acagaaaaag tttgctgtcc tagaaataga
    16261 tgaagcaagt ttgagcaaag tcacagaata tatgaaacca gaactgttct tatttacaaa
    16321 tattttccgt gatcaaatgg atcggtatgg agaaatctat acgacctata aaatgattgt
    16381 agacggtgcc gcaaagtcac caaatgcaac gattatcagt aacggtgatt caccaatctt
    16441 caattctatc gaaacagtca atcctagaaa atattatggc tttgaccatg aagaagatcg
    16501 tgaacaaatg gctcattaca atacagacgg tgtcttatgt ccaaattgtc atcacatctt
    16561 gcattataaa atgatcactt atgcaaactt agggaaatat tactgtccaa attgtgactt
    16621 caaacgtcct gaattggatt atcgattaac tgatatggta cggatggaca atgtgtcagc
    16681 agatttcgtg atcgatggtc atgaatacgg aatcgaagtt ggcggtatgt ataatgtcta
    16741 caatgcttta gcagctactg cagtggcaga gtacttcaat gtcgatcctg ataaaatcaa
    16801 acaaggttta ggatatgacg agaaagtatt cggccgacaa gaagtcatca atatcaatgg
    16861 gaaaaaatgt acattagtac ttgtcaaaaa tcctgtaggt atcaatcaag taatcgatat
    16921 gattggtcta gctccttatc cattttcttt agtggcttta ttaaatgcca attacgcaga
    16981 cggaatcgat gtcagctgga tttgggatgg taattttgaa aactttgccg atatggatat
    17041 cccagctgtt atttctggag gagaccgtca tacagatatg actttacgtc taaaagttgc
    17101 cgggattcca gaaaataaat tgacagaaac aaaagaatta ccagaagtaa tcgaaaaaat
    17161 caaagaattg ccaactgagc atgtctatat tttagctact tacactgctg tattgcagct
    17221 tcgtaaagaa ctagctgctc aaggatttat caaaggagga atgaaccgtg gctaattatg
    17281 aactcaatat cgcccacctt tatggaaatt tgatgaacac atatggtgat aacggaaacc
    17341 ttttgatgtt gaaatatata gcagaaaaaa tgggggtttc ttgccatacg gagatcgtta
    17401 gtatacatga accttttgat gctgataaat atgacttggt tttctttggc ggtggtcaag
    17461 actttgagca attaatcatt tcaaaagaca tccagaaaaa gaaagattct ttgatcaact
    17521 atatcgaaaa tgatggcgtc atgttagcca tttgtggtgg ctaccaattg ctaggtcact
    17581 actacatggg agctaatgga gaaaagatcc aaggaatcag tgcactggat cactacacgt
    17641 taagtcagga aaataaccgg tttatcggtg atatcgttat ccataacgaa gaattcaatg
    17701 aaacttatta tggatttgaa aaccacaatg gtcggacttt cttaggtgaa ggtgaacgac
    17761 ctttggggaa agtggtacaa ggtcaaggaa ataatggtga agaccaatct gaaggtgtca
    17821 tttaccgaaa tgtcttcggt tcttatttcc atggacctat ccttgcaaga aatgagaacc
    17881 tggcagctcg tttgatccgc ctagctttag aacaacgtta cggtaaagaa ttggatctgc
    17941 cctatcttga aggacaaaca gcctagttat tcgaaaaagg ggaatgtgac aaaaatttcg
    18001 tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac ggttcacgtt
    18061 ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt gaatcaattg
    18121 ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat cttcaggatc
    18181 atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca agttatgttc
    18241 ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac gtaatggtgt
    18301 ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat gaatgatttt
    18361 ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca gcaaacttcc
    18421 tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca ttgcttcatc
    18481 aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac cagtattgac
    18541 actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa ccccttttaa
    18601 ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga aaggttccac
    18661 ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga ctgctgcatt
    18721 tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat acacttcttt
    18781 ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta aagatgttgt
    18841 gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa taaaaggtaa
    18901 gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt ggattacttt
    18961 ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt tttcttttga
    19021 ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa tgatcgtgtg
    19081 attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc caataaatcc
    19141 tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc cttaagcaat
    19201 tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga caacattgtc
    19261 catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat ctccgactcc
    19321 tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa aatctacacc
    19381 atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg cttcatctgt
    19441 tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt ctctatctac
    19501 catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct gaccgcaata
    19561 gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt cgaatggcaa
    19621 actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc aatcgattcg
    19681 tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg tgcagccatc
    19741 tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc atcttttaaa
    19801 tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa tatatctttg
    19861 agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag tagaatctct
    19921 ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc aatttcagaa
    19981 actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg aggtgaattc
    20041 aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc tctttcttgt
    20101 agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct gattgcttga
    20161 ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat cgaagcgccc
    20221 atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac gccagagaat
    20281 gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc ctttgtctct
    20341 tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa aattactgca
    20401 gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg aaggtaagaa
    20461 ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg cgctcttagt
    20521 ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat aactgataag
    20581 aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc gcctaaaaag
    20641 tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa caaagtaaaa
    20701 gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag aatagatttt
    20761 ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt gattgcatta
    20821 ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat caaatgggca
    20881 gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac aactggacca
    20941 atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga aaaattattg
    21001 actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa tttcttgatg
    21061 gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat atctgttccg
    21121 acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc agcaattact
    21181 tttccgccag tataaccgat caataaagga atcagataag ttagcattgg gcttaccatg
    21241 gtagcgaaag cctcatttgg taaataacca tctgggataa atagagccgt taatacaccc
    21301 caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact gcctaatttt
    21361 tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat tttgtctctc
    21421 cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat agtcgtactt
    21481 tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag atttcggtat
    21541 cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata tgcaaacaat
    21601 aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta aattttatat
    21661 tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg gtaaaaaacg
    21721 tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt tgattgcccc
    21781 gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc caacaactta
    21841 tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc gccggtcttt
    21901 cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg attatttgac
    21961 tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc aacgattgaa
    22021 gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga aacaacatgt
    22081 cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg aaccgttgaa
    22141 cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat ggcagaaaga
    22201 ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga tttgttctaa
    22261 agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg aagagatttt
    22321 agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt tgaacgcaat
    22381 ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt gctctttgca
    22441 ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact gaatcataac
    22501 tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga gaaagaattt
    22561 gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc aaaaaacaca
    22621 gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat actggatcga
    22681 ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg tgtcgagttg
    22741 ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt gccagcgcat
    22801 ctctctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag ttttttgatt
    22861 ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag aaagcaggta
    22921 atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat ccgttttacc
    22981 atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt ctctatacta
    23041 gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat cattggtcag
    23101 gagatacgaa tcctgccgat gtatgagtat cttttccaga gctttctgtt cctgttgttg
    23161 tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga acattctcta
    23221 gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt gttttcttcg
    23281 attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga ttttcaaaat
    23341 gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac tttcatgaat
    23401 ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat gggcactatc
    23461 tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa aaccttgtca
    23521 ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa gcttgcatcc
    23581 gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg gttgaagtta
    23641 ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt ccatgttcaa
    23701 aaatcatttg gacttcttca atggtttccg catcactgag cgccattact tggtcgatgt
    23761 ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt tttaaagcca
    23821 ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc acaccatcag
    23881 gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca gtcccatgtg
    23941 gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg atcattgatt
    24001 ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc tcggtcaacg
    24061 cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt agctgtatca
    24121 tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa ctgataaacg
    24181 atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat gattgctcca
    24241 ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc cagcataact
    24301 aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa cggatgtttc
    24361 aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg gaataacccc
    24421 atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag ccggcgatga
    24481 accacctctt catccgttac tagtccttca ggaaaataag gaaataagct gcgaatcgtt
    24541 tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag taattgattg
    24601 atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca ctcgttattg
    24661 ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc ttcttttagc
    24721 tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc accgtatcct
    24781 ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat tttttttaaa
    24841 tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt caatcgtagt
    24901 tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact gatattgctt
    24961 tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc gatctcttct
    25021 tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc attcgcaact
    25081 tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc ttcttgtaaa
    25141 acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa agtttcatct
    25201 tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt tactaaataa
    25261 gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt cgtgaagtca
    25321 tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt tgtctcagaa
    25381 gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc cagactatta
    25441 tatgtctcat ttgtcagtaa atgatttttc gtcacacgct ccatcgtcaa tgtcagcatc
    25501 aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat caattcttcc
    25561 ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa aaaataattc
    25621 agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc taattctaat
    25681 acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta tccctggttc
    25741 cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat atctgcataa
    25801 aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa cggctctgtc
    25861 gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa gtcattactt
    25921 ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc aatgacaaat
    25981 gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac ttcaagatta
    26041 tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat tttttggata
    26101 gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct gggcgataaa
    26161 tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta cccaatgttc
    26221 aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta gttctctttg
    26281 tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt ttgagataac
    26341 aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca tagtttgtga
    26401 gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgacgttt tttgttctgt
    26461 gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt cttttattcg
    26521 tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac tttgaggtac
    26581 tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat ttacttggtt
    26641 attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg ctgctaattt
    26701 ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc aagcaaaaat
    26761 gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg ttttgttctc
    26821 tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca caaatgtact
    26881 gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg caaaaatcac
    26941 gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt gaaatatcaa
    27001 cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca gcaagacaaa
    27061 tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc cactcgcatt
    27121 tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta aaccaggtcc
    27181 aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga attcgattcc
    27241 taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt ttagaggttc
    27301 taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca caacggaggc
    27361 gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa ttatacaaat
    27421 aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc cagccttgaa
    27481 tttcacatgg aatctagaaa caaattgttc atatacgatg ccggcaagcg gcccgataat
    27541 catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag aagcaattgc
    27601 accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac tgatcaagat
    27661 gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt gcaaatcgcc
    27721 agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac ttagatttgg
    27781 catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag acacgcgact
    27841 gctgttgttt gctttccatt tgtattccat ctttttcacc agacttctat ttattgatgg
    27901 acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc agctatctgg
    27961 gaactatttg cctggttcag acgcaatgat tgctagttca ccttggaccg cccactcaga
    28021 aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt ttccgtcaac
    28081 gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt gttttttgaa
    28141 agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt ctaaataagt
    28201 gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg ttttcgcagg
    28261 gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc cttgcgcatc
    28321 ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg tttctaaaat
    28381 catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga aatcaccttt
    28441 tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca tctcagctag
    28501 ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt cagcatcgat
    28561 gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat aggcatcatc
    28621 tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca ataatggaaa
    28681 cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt catctaatct
    28741 tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg cactgatcgc
    28801 ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac gtaaacgact
    28861 tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct gcataattca
    28921 aactcctctc atttttcata gctattatag cattatttcg gtaataccgc tttctctttc
    28981 aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg atcgacagga
    29041 tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat caagaaaaac
    29101 gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc cttttttaag
    29161 tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag taaagaatca
    29221 atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa atacatttgg
    29281 acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc atgttcagcc
    29341 aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga aggcagttgt
    29401 tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat cattcctaat
    29461 ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat ttcctctagc
    29521 atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc tagttgctca
    29581 cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt atctctgttt
    29641 ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt gattgtctga
    29701 ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt tccatcttct
    29761 aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc tgcatcaaac
    29821 aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc ggctgtaaat
    29881 gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa tccttcttcc
    29941 atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg cttttctatc
    30001 agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt tttacacaaa
    30061 aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt tctatctcta
    30121 gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc gttaagaaag
    30181 aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct cactgacgaa
    30241 ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt tgtctaagaa
    30301 gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt cagtatcgat
    30361 catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa ttgcgcctcc
    30421 gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga tgccctcttc
    30481 ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt ttgttaagtt
    30541 agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac tgaatttttc
    30601 ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc tttggtcgtt
    30661 cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga gcaaaatccc
    30721 catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac ggatacgaca
    30781 aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt tcagcttttt
    30841 gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca tttcacttcc
    30901 agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat caaagccaac
    30961 aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg ctacactttt
    31021 taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc tctacgtcaa
    31081 gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt tgttaaaaaa
    31141 aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa aactcttata
    31201 aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag
    31261 ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    31321 ttaaggaaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa
    31381 atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
    31441 cgtttaccaa gaggttgtga gcctgccgtt taactccgag tcacaaggag caatttcaaa
    31501 aaacagcttc tcaggtttgt tgactattgt gacttgtgac cgaggagttg gcaggagaac
    31561 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gatacaaaac
    31621 ggaaaaatag cttctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg
    31681 aacaccgttt attcggaaaa agggactgtg acacgacttt tgtcacagcc cctaaaaaaa
    31741 ataataaatt tgtactattc tatgaagctt cttcttcaga aaactgactg ttataaagat
    31801 ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt tcctgtttct acgatcgaac
    31861 catgattcat cacaataatg ttgtctgcat cgcgtattgt agacaagcgg tgagcaacaa
    31921 cgaaacttgt ccggttttcc aataggcggt tcatcgcttt ttgaatcagt atttctgttc
    31981 gtgtatctac actagatgtt gcttcatcaa ggatcaggac atctggattt gctaagaatg
    32041 cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat attgcttgca tcctcgttta
    32101 atacagtgtt atatccttct ggcaatttac gaacaaaatc gtctacgtga gcagctttag
    32161 cagcctcaat gatttgttcg tctgttgcat cgtcgttccc atatttgata ttgtcataga
    32221 tacttcctgt aaacagccaa gtatcttgca gtaccattga aaactgtgcg cgcaattcat
    32281 cacgtgaaag atcacgggta tctacaccat cgtaacgaat actacctcca gaaacatcgt
    32341 agaaccgttc aagaaggttg atcagtgtcg tcttccctgc accagtcgga cctacaatag
    32401 cgaccatctc accaggtttg acatccaagt taaaatcagt cattagcaag ttatctggtg
    32461 tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg tgtatctttt tcttctggaa
    32521 ggccagattt ttcattggac atctcttctt catccaacac ttcaaatacc cgttcggctg
    32581 aagcaactgt tgattggata gtgttcatca aacttgcaag ttgagtgatt ggttgagaga
    32641 actgtgttgt atattgcaag aacgcttgaa catcccctaa tgtcatgttc ccatttgcaa
    32701 cttttactcc gcctagtaca gcgacgaaca catagcctag atttttaacg aaattcatca
    32761 acggcatgat gattgccgaa ataaattgag ctttccaacc agcggcatac agattctgat
    32821 tttctttttc aaatacttcc tggtcttttt cagtatgatt gaatgtttta acaatggtat
    32881 gaccaccata tgtttcttct acttggttat tcaataaacc taaacttttt tgctgatctg
    32941 cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat gatcaagctt aaagggatca
    33001 tcaataacgc gactaacgtc aattgccagc tgattgaaag catcatcacg atgaccccaa
    33061 tcaatgtgac tgcactagtt acaaattgag tcaaattttg ctgcagcgtg cttgcgatgt
    33121 tatccatatc attgattgct cgagacatga tatccccatt tgtatgtgta tcataatagg
    33181 aaatcggtac acgattcatt ttggcttcca aatcatgacg taattcataa actgttcgtt
    33241 gggaaacacg tgtcataata aattgctgta aaaagttgaa tacagcagaa atcaaataca
    33301 tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa gtctatcgga aattttgtta
    33361 cttgttggcc ttgttgcatc tgttgagcgc cagccattac acctttgaat atttctgtgg
    33421 ttgctttccc caagaccttt ggtgtttgga tttggaagat cacggctgct attgctagta
    33481 caaatacagc gatgatcgca accatacgtt tagacatata gccaaacaat cggcgtaccg
    33541 ttccccagaa atttttaggc ttttcacctt ttacaccgag atttctgcca ggtccaccat
    33601 gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt tgttttactc atgctaagtc
    33661 ctcctctctt agttgtgaat gaacgatttc ttgataagtt tcgtttgttg ctaatagttc
    33721 ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc acaaggatca tatctgcatc
    33781 cattactgta cttactcgtt gagcaactaa aacggtgata ctttctttca tattcgtttt
    33841 caatgcttgg cgaagattgg catccgtttt gaaatccaac gctgagaagg aatcatcaaa
    33901 cacatagata tctgctgttt tgactaatgc ccgtgcaatt gccagacgct gtctttgtcc
    33961 accagagaag tttcctcctc cttgttccac atggctatct aagccatctt ccatctctga
    34021 aacaaaatct ttagcttgtg cgatttctaa tgctttccaa atcatttcat ctgttgcatc
    34081 cggcttacca tactgcatat tgtctcggat cgttccagag aacagaactg ctttttgagg
    34141 aacaaagccc attaattctc gcaaattata ttgagtcatg tcactaatgt ccgttccatt
    34201 tatttggatc gaaccagatt cgatgtcata taatcgaggg atcaaattga ctaaggtcgt
    34261 tttacctgaa ccagtccccc ctatgattgc gacgatctcg cccgacttgg cttggaaatc
    34321 aatatcttcc aatgcaagtt tttctgctcc atgatatcga taattgacat gattgaaggc
    34381 caatgttgcg ttttttccag caaaagaaac tgttttagga ttttcagggt catggatacc
    34441 gattttttca tccaacactt cattaatacg gtcagctgaa gcttgtgcac gaggcaccat
    34501 gataaaaata gcggaaagca tcataaagct gatcaagatc tgcatcgcat aagtcataaa
    34561 tgcaatcaga ttaccgactt ccaaagtcat atctgctatg tagtgtccac caaaccaagt
    34621 gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt ggcatcatca atgcgacgat
    34681 cgtattaact ttgattgccg tatttgtata atctttattc gctaaatcaa aacgattttc
    34741 ttcaaagcgt gtcttatcga atgctcgaat cactctgacc cctgtcaatc cttctcgaaa
    34801 taccaggttt aaacggtcag ttttcttttg catacttttg aataatggaa cagcaaagta
    34861 catgattccg ccgatcaaaa taatcattac tgggatgaca actaagaaga tttttgtcaa
    34921 ctgatgatct ttttgataag caagtatgct cgcgccaatc aacgtgattg gtgcattgat
    34981 catcatacgt aaaaacattt gtgtaaccat ttggatttga gtcacatcat tggtcgttct
    35041 tgtgatcaaa gaggccgttc catatttatc aaacgcatct tttgtcagac tctctgattt
    35101 tttatagaca tccgtacgca attgcttacc cagtttctgt gattcacgag tagcaaaaaa
    35161 tgtatttgcg atagcagcta agatactgat caaagaaaag ccgatcatca caaaacctgt
    35221 atgccaaata taatcgacat ctccttgcgc taccccttta tcaataatat tggaggtaag
    35281 tgtcggcaaa tataaatctg ccagtacttg gatcaccatg aaaactgcag ctgcaattgc
    35341 tgaagtcaag gagatccgtt ttacgatttt tagcatctag ttcccttctt tctatttata
    35401 tactccgttt ttcagccaag aaagtttcaa attgaattca ctgattgctt gctctactga
    35461 gtaatcttca gatgttgaaa ataagcgata cgaatgcgca attgttgtaa atacagtatt
    35521 catcactaat tggatcagta attccaattc atgatcattt tttactttca gcttcgtccg
    35581 atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt tctttatgtg gatcgtgagg
    35641 cgttttttgg cgactcgtat gaggagcgac ttttcttgag gcacggtaat ccatgttcat
    35701 aaacaagtta cgataaaaag aagcattctc gccttccaaa atctcataaa tcattttaga
    35761 aaaataggat tcgaatcctt tgaatagatc accatcggat tctttcatta gttgtaacat
    35821 atctttagag ctgtcacgtc tcattgtctc aaagtaataa aaataaagat cctctttatc
    35881 ttcaaaatac tgatagaaac tgcctctagg gatatccgct aatttgacga tattagcaat
    35941 cgaagcttct tttaagggta ctctagaaaa ctcgattctc gcagcctcga ttagtctttt
    36001 ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc atatggattc tcctttcttt
    36061 caagatgaca cattgtcata tgacgctatg tcattataac acctcctacc tgttttgcaa
    36121 gacatttgca tagttttttt cattatcatc cgatacatta aaaaagtgat aagaggaatc
    36181 cttccatttg aattaatgag agttgcccat taaaatagcc acatactaaa caaaggatga
    36241 gataaatggc ccaatcaatt agtgtccaag acttcaataa attgcctctc gatgctgaca
    36301 gcatcgtttt agatacaaga aataccttag attacacaaa aaaccactta agccactcgc
    36361 tttctatccc cgctgctatc ttgccaagac aattaaaaaa tctagatcca aagaaacttt
    36421 attatattct aagctataca ggcaggcgat cagaagcatt agccgatcaa cttactgcta
    36481 atggttttcg tgctgtttat atcattggtg ggatggaagc tcttcagtcg agtgttgctt
    36541 gaatattgct tgttagattc aaaagaggct gtgacaaaaa atcgtgtcac agcctctttt
    36601 ataccctgtc tttttttagt tatttattct tcaaccttat caatatgact tgatagcctt
    36661 ttctttttcg agtaaactga ttttgttagt catagttata acaatcatag caataaaagg
    36721 agaaggtaag catatggcat ctgttttagt agtcaaaggt catcccctaa ctgcagaaga
    36781 atcgcgcaca gttaaggcat tgacgtcttt tttaacaagt tataaagaaa atcatccaga
    36841 tgatgaagtg actgttttgg aactttatcg cgatgatatt ccggaaatcg atgaagaatt
    36901 attatcaggt tgggaagcat tacgtgctgg tgcagaattc acttccttat cagaaagtca
    36961 acaacaaaaa attgctcgtt tcaatgaatt gactgatcag ttccttgctg ctgataaaat
    37021 cgttatcgca aacgccttat ggaatctaaa tatcccaaca aaattaaaag cttggttcga
    37081 taccgtcaat gtagctggaa aaacgttccg ctatacagaa aacgggccag aaggattagt
    37141 gacaggtaaa aaagcactgc acatccaatc taatggcggt gtatacaacg gacaagactt
    37201 tgcctctcaa tatgtaaaag gtattttaaa ctttgtaggc atcgatcaag tagatcagct
    37261 attcatcgaa ggaatcgatt atgaccctga ccgtgcagat gagttgatgc aaaatgcatt
    37321 agataaagct gctgctttag gtaaatcatt ttaaaaattg agataaatgc atacaaaaga
    37381 gacagaggaa aaaatcttca aagcagcatt tgtgccttgg atattttccc atctgtctct
    37441 ttttgttatt ctttctgctt tttcaacgga ttttcttcat aaagagaaga gattgttgtg
    37501 taccattcaa aaacatgagt aatcactact ttagctgctg cataaacagg aatccccaag
    37561 atcacaccga tgattccgaa caatttccca gaagtcagca gaactaataa aatcgttact
    37621 ggatggattg ctaactgatt ccctagtact aatggagaga tcaagcgtcc ttctattgtc
    37681 tgctctactg caaaaacgat cagtactttg atgatcatga atggacctgc tacaatggct
    37741 aaaaagatag ccggaatcat cgctaaaaat gatcctaagt aagggatcag gtttaaaaaa
    37801 cccgcaataa tccccaacgt gacagcatac tctaaaccaa tgaccgcaaa accaatcata
    37861 aacatgattg ccacagcaaa agccacagtt agttgtcctc ttatataaga agaaacttga
    37921 ctattcattt cactcagcac attcaacgtc ggtttttgca tgcgtgtcgg taaaaatttg
    37981 acaaaataag gtgctaattg tttaccgtct ttcaatagat aaaataagat aaacggcatc
    38041 gtcagcagag ccactaaaat agtcgctacc gcgccgacaa aacttccaag actctgaact
    38101 gtactcttag atatatcttg gatcatatcg cctgccgaat tcatgaactt atcacctgct
    38161 gtttccaact gttcgcgaaa ttggttgaac agcgggtccc gcaaaatctc ggttaattta
    38221 ttatcaatcg tatctacata ttgagggaag ttactgatga agctcactgt ttgttcctga
    38281 atcttaggga tgatcactac gctcccccat gcgattagtg caacaaccaa aacaaacagc
    38341 ccaatgatac tgtaaagcct tggtactttt cgcttttcaa agtaatcaac taccggattc
    38401 atcaagtaat acaagattcc tgctaaaatg ataggcagac ctacgattgc taaaaattgc
    38461 cagattggtg caaacaaata tgaaactttt gtaaataaaa agacgatcag caatattaac
    38521 aatacaacaa gtaaggctgt gactacttga ttatttaaaa accagcgcca aaaccacgat
    38581 accggtctgt tcttcttatt ttttccttcc ataattcctc ctgttagtta taaaaaattt
    38641 ggcttcccaa accaatagca ggcagtctat gcggatgaag atagattcca tttgcaattt
    38701 gtccttcttc tggacaagca tcaaaaatca atgatacgtg agctactgtc tttaaattcc
    38761 cattggccat cggaccgaca taatcgatcg tatatacctg atcatcaaaa gcaattgttc
    38821 cgcctgcttt caaagaaaaa ggttcgtctt ttgatacttc ttgaatcacc gaatattttc
    38881 gtaaggcagc tgttgctgtt ttatcgaaca aaatcagcat gggttcttcc ttgtttattg
    38941 cttctgtccc aatttctgta atctttgcct gaatcattgc actctctcct ttaattgctt
    39001 tcaaaaacag tataacacat tctcattctt tagccgtgtg gaataattat gtgatagaat
    39061 aaaaacgaaa actatttcat tacaattagg aggaatcagc tcatatgatc gaaaagataa
    39121 ttttaggaac ctatacgcgt cgcgaaagta aaggaatcta tacgatcgac cttgatacag
    39181 aaaaagaaga attagccaac ctttcattag ctgttgaaga aaatagccct acctatgtag
    39241 caaaaagtaa agctggcaat ctttacacag ttactagtgt ggatggatta ggtggtgccg
    39301 gtgcgtacga taaggatttt cattttttga atgcggtaac agagtctggc gctcctttgt
    39361 gttatgtagc ggttgatgaa gcacgtcaat tactatacgg ggcgaactat cataaaggtg
    39421 aagtaaatgt ctaccatata ttaggagatg gcgaactgaa agcagcagat tcaatattcc
    39481 atcaagaagc gacaggtcct cataaaaacc aagatcatgc gcatgtccac tatacggatc
    39541 tgactcctga ccaacgatta gtcgtatgtg atcttggtac agatcgtgtg tatacttatg
    39601 atgtgtccga aaacggcaaa ttgaatcttg taactacctt cactgcagag ccaggaacag
    39661 gccctcgcca tcttgtcttt catccaaatg gacaattcgc ttatttattt ggtgaattag
    39721 acagcacagt ccgcgtgctt tcttataata aagaaacagg tgcttttgaa gaattacaga
    39781 aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg aggggcagct atccgcattt
    39841 caaatgatgg caagttcctt tatgcttcta accgaggaca taactcgatt gctgtattcg
    39901 cagtttttga agatggttcc cacattcaaa acaagcaaat catctcaaca gaaggggatt
    39961 ttccccgcga tttcgcacta gatccaagca ataattttgt cgtgtgtgcc aatcagaaca
    40021 ccgataatct gacattatat cgccgtcatc ctgaaacagg attactggaa ttactgaaaa
    40081 aagatgtcta tgtaccagaa tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga
    40141 atgaaatgaa tcagcaagac gtattagaca gactacggga agaactaaat attcctttct
    40201 ttgatgggaa aatcgaagat aaagagtata gcgaagagga atatcagaaa ctcaaagccg
    40261 atttacagaa ctactttgaa caatatgtac agaatgtaga aaactaatca gctttccata
    40321 ctatctgaaa agaacatcag gacaagactt ttccgtctat cctgatgttc ttttctacaa
    40381 ataaaaaacc gccgagatgg ttagactcga cggaaaagga gtatttactc gattttgagt
    40441 aagtacaagg ttgttgttgg ttataggtat gtcattatca taaaggattt tgatcactct
    40501 atcataaagg taatattaca tttatattag ataactctag tttttattgt tatcaaagat
    40561 ttcttttatt ttttcaacaa caaattagtc attttttgga tggcagctgt caattggtct
    40621 gtcgcttggt cttgttttgc ttcagatgta ttgaatagat cttcatctac ccctgattca
    40681 agatcgatac tttggatcat ttcttcacag cctgctacat atgaaagata agctttttca
    40741 aaatttttat gaatgcccat tgcttttgct ggtggacgca aagtactgac tttcataagc
    40801 attgcccgat atttttttgt tccttcttca aataacagga tgatctcttc aagagtcgat
    40861 ttgtccagtt cacttgtttt tccttcatca attgcgtttc tgatcacttc atatttcgga
    40921 tgcattcctt ctccgattgc ttctgtttcc ttgacgattt gatcgatgga ctgcacataa
    40981 tagccaatat tcggtaacat gttcgttttc ctactttctc cattgttgtt attatttatt
    41041 ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa tttaatggtt tctatacagc
    41101 tcgccattct tattcgtgct caaagaaacc atctgctaaa ctatattgtt tccctgcgac
    41161 aatgtcatat tggatcaatt gatattcgtc gtaaatctct tgttgcgagc gattgaattg
    41221 cgcttcttta taccactgtc cgttttgata gtacagttct cgttcaaacg ccggaagttc
    41281 ctgttctaat gataaaagca gctgataaaa gccatttgtt gtctgattcg tctgttccat
    41341 tagattcgct gcaaaataaa acggactggt gattgcatcg tgagtcgttt gcttttctaa
    41401 ttctccttta ctgtcgaaca taagaaattg tgtttcatgc aatgtttgtt tgtcattttt
    41461 tgcttggatc gtatctgaat agatccctgg aagatgatcc ccccaaaaaa caactaaagt
    41521 ccttctagaa agattcttca attcttccgt gaactgcttc agtgccgtgc tgctatagga
    41581 aatatcttgc agataattct ctaagtcaag tgtcccactt ccatctgaag tttttgcagg
    41641 atagtctaga ttatcatact ttccatcgta gggcatatgt gtctgcatcg tgactaaatg
    41701 aatgaactga ggtgtttttt cttccttgag aagtgtcatt acttctttgt aagctgactc
    41761 atcagaaata tatggattat tctctatcgt atccgtatag gtcatcgtac gctcgctgat
    41821 gaactggtca aatcctaatg tctgataaac atcctctctt ttatacatcg aagtattgta
    41881 tggatggatc gctgtcgtct ggtatgattg tgcgtttaac gctgaaacga tagaaggaag
    41941 ctgatccatt tttggtacaa gcatcgtgta aggggtggtg agttgcgcat taaataacgc
    42001 catagaaaaa ccagtcaatg cttcaaattc gatatttgct gttccaccgc catagttttg
    42061 agaaagcata ttgccactat atgtttgatc agctacttca taataatctg ccaatggctc
    42121 accagatact tctacgccgt ttaatcgact aggatctgag aaactttcac tcatcacgaa
    42181 cacgatgttc ggcgactctt cctctacagt tttatttttt tcatcggcta gcttctggta
    42241 tttttcagtg atttctttga tcttttcttt cgaatatcct tcaggttcat ccattggttc
    42301 taccttcaga ttgtacaaaa acccaccgat aaaaccagtg ttatagtagt tcattttctg
    42361 gctgtaaggt atccatagtg ccgttttatt atatgctttt cgcaacaaat tatcgggatt
    42421 gttgaaatta ctgatatacc ctagcagtcc aattgccaca agcagtgtcg ttaatcggat
    42481 caattggaat cgccttcctt ttttcaggct tcgaaacatg taccagcaaa acaatcctat
    42541 cacggttcct gcggcagcta agatcactgt aaacagcata gtgccggtca tatctttcag
    42601 caatccgatt tcagtgatca tcttcaaatc gtctggataa atcggttctt gccgataact
    42661 cattttcata taatttgcaa aacctaatac accgatggct acgacatacg taagggaacc
    42721 taaagggaca gatcctgcta gtgcaatcag aaaaataaga ataatcagca agacgagaca
    42781 ggctaagaag aatttttccg tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga
    42841 tagatcattt tgacaccact gcaaaaataa attactaaaa acaacaagaa aaaagatact
    42901 gatgattgct gctgttcctt ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata
    42961 tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag aatgttgctt ctcttatttt
    43021 agttgtacga aattcgaaag taaagaacgg aaagaagaat taatgaaatc caaatattct
    43081 ctttctttat ggcctttctg gtttctttaa taagcggacc tttcgtactc cagaaatttt
    43141 ttctagctgt tgtttcactt gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa
    43201 tatgtagaca tgctcattct tatggttacg cgatatatct gcgatcgcta gctcgttctc
    43261 attcagcaga gaaaatatct ctgctaatat attggttttc ttttgataaa atatagtgaa
    43321 tcgaaacggc gcttggaaaa acattcgtgc ggatggataa ttgaccgctt ctctaaccgt
    43381 accgaacaaa agataatctt ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc
    43441 ttcctgagtc gatcccctag atgaggcaac ataagtatac ggtcattttc taagaactct
    43501 tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc ttgcataaca
    43561 gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc ttttttcatt
    43621 ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg cagcatgact
    43681 acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac ataatccaac
    43741 tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca acttagcgct
    43801 acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg caattctcgt
    43861 cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc tgttagtgtt
    43921 tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca gcacaaaaca
    43981 agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt cccgttttca
    44041 gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat tgctagcaaa
    44101 tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc ttcggctggc
    44161 aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg ttctggaata
    44221 gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt atctcgaaat
    44281 ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga gcagttagag
    44341 cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa gccttatttt
    44401 cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta gctgacattg
    44461 ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc ttcttatttc
    44521 ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt ttccaactca
    44581 actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac aacggtatga
    44641 tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg tttagttttc
    44701 cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa ggcttctact
    44761 tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac taccccttct
    44821 cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat tgataaagga
    44881 gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat catatgggat
    44941 tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat gaatcctgta
    45001 tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt ccagtcgcct
    45061 tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc aggtagttgt
    45121 ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc ttcttctaag
    45181 aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc ccatgtcttt
    45241 tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa taattgatcg
    45301 acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc aaaataatca
    45361 acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac acccacgttt
    45421 ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata ggctactgca
    45481 acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta gccaacgacg
    45541 attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc accatgagcg
    45601 gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct ttcgttatat
    45661 tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat ccacattttt
    45721 gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg ataattgacc
    45781 tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag gcggattgga
    45841 taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg tttctttact
    45901 tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca aaattctttt
    45961 tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta atacgcattt
    46021 tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc ttctgaaaat
    46081 gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac gactgcgttc
    46141 attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg taaaagtacc
    46201 cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat aacttttcct
    46261 gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc acaaggtacc
    46321 gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa tcctgcttgt
    46381 gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg aggaaatgaa
    46441 cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc ttcaatgata
    46501 tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata actgactccg
    46561 ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt tccccatgta
    46621 tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc ttctttcagt
    46681 tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg ttttttcaat
    46741 gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt cttctgctga
    46801 aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga agttttctaa
    46861 agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc acgctccacc
    46921 ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta gaatccgctg
    46981 tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa tttggacatt
    47041 ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg ctaaatggat
    47101 ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata atggccttcc
    47161 ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta atcccccgca
    47221 atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat ctctgcttat
    47281 ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat tgatatccga
    47341 agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag tgaccatgtc
    47401 gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga aggcggaagt
    47461 accagccgaa atgttccctg tccctttggc tacactgttc gtagcgacca taccggttcc
    47521 tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg tagggtcgat
    47581 cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgctacta gaactttagg
    47641 caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac caaaaatatt
    47701 tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc ctgatgcatc
    47761 tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg caagcgttgt
    47821 caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat acaaatgagc
    47881 gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc gttctgcttc
    47941 ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct tgctatcaaa
    48001 tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg taagattctc
    48061 gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca gtcctttcca
    48121 aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc actcataact
    48181 accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc tagttgatcc
    48241 aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt gcgcatttac
    48301 ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc gtctttttag
    48361 tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcagt tatttacaag
    48421 aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa ttgcttacaa
    48481 aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga aaaaagaaaa
    48541 aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc tatttggata
    48601 tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg gtttggcagg
    48661 taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga tccttggcgg
    48721 ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc ttctttctgc
    48781 acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg gttcttattt
    48841 tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg ccctagttcc
    48901 tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag ggatcaatca
    48961 agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc taaaagggtt
    49021 gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg cgctgatttt
    49081 atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat ctggccgctt
    49141 ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg aacaggaatt
    49201 cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt ggcatacttt
    49261 gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag tattccagca
    49321 atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa aagcgactgg
    49381 gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt tagttgctgg
    49441 ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat tgaaaatagg
    49501 cggtagcgtg atgggacttt ctttcatcct accagctgtt ttaggaactg tactggatgc
    49561 tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat tttattcttg
    49621 cacatgggct ccactgacat gggtaatcgt aggcgaaata ttcccactag ctgtccgcgg
    49681 gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag ttggcttgct
    49741 ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt ttgggatcat
    49801 ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg ggaaatcttt
    49861 agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt ctatccatca
    49921 ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga accgcttagt
    49981 ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa tattgtgact
    50041 tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa acgttcagcc
    50101 ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga gcaataagag
    50161 gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt caactagtac
    50221 aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga cgatccaccg
    50281 tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt ttttattccc
    50341 taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg gaatatgata
    50401 gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat agcaaatgat
    50461 gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga ttgtttctcg
    50521 tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat actgttcaca
    50581 cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt ttgtgatgaa
    50641 caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac cgactttcac
    50701 atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga tcatcaccat
    50761 cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat actgattact
    50821 tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct tcaaacactc
    50881 tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc cagattctcg
    50941 taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga gatatgtggt
    51001 gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat agctgtcatc
    51061 tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca acacagcgat
    51121 tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga attctgaggg
    51181 aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct cttctgcaat
    51241 atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata tatcataacg
    51301 agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag gcatacggaa
    51361 caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc gcgttcgcgt
    51421 gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga gcatgataga
    51481 tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca ggaccaaatt
    51541 gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat gattctttta
    51601 ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt tcatctgttg
    51661 cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct ggaccttcat
    51721 ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg atctgcttcc
    51781 cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc ccagtaattt
    51841 caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa gttgttccgt
    51901 ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa tctgtattca
    51961 ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat tcatcacgta
    52021 tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata taatggactt
    52081 caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat acaatcaggc
    52141 ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc acttggtagc
    52201 ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc caaggatctg
    52261 cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat ttcctcacat
    52321 tttctttatt ctaattacat tccaagagta tttttctatc cctacattta attcgtcatg
    52381 tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt cacgtgtatt
    52441 tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta gcaaattgaa
    52501 atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga aaatagtcag
    52561 ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta tcttttcaca
    52621 atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat atttcgataa
    52681 gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat ctgtcagtat
    52741 taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat ctgcgtgtcg
    52801 taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa agtaacacca
    52861 atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg catgtacatt
    52921 ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt ttacataatc
    52981 acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg caaggtatgt
    53041 cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat catacttatc
    53101 aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta atgcgatacg
    53161 atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga ttgtttcaat
    53221 tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac catattcata
    53281 agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac accataattt
    53341 aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga aggttccttt
    53401 tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg ttcctaaatt
    53461 caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt cgtggagtcc
    53521 aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc gttgttcttt
    53581 cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag gatagcgaat
    53641 gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc cgtcaccatc
    53701 agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca tatgctcaac
    53761 aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc tgatttttac
    53821 tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca agataataga
    53881 tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa gaaatatgag
    53941 cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat caaaaaatac
    54001 tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt cagaaatcac
    54061 atatatccag gataaaagaa acatggaata aataagtaaa actacatatc cactaattga
    54121 aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa tgatcgttaa
    54181 cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga aaaaataaat
    54241 gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag cacatgtagc
    54301 aggcacgata gtaataacag gcaaacacac gattacccag agaataccta ataagatgta
    54361 atcagaaacc acaatcaact gtccataaag tttagtctca actattttca tcttcctcac
    54421 tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa aatataaacc
    54481 actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata aaaagaaatg
    54541 ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga actacttgtc
    54601 aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat ccctaatgat
    54661 aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa ctcacttaat
    54721 ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa ggaacgcatc
    54781 atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata agtatcaagc
    54841 atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa tgggatcatc
    54901 attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat acgagcaaat
    54961 gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc agcaaaaatc
    55021 actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat atagttatct
    55081 agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt ttcgtgttta
    55141 aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat aactagcata
    55201 ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac agtaactgta
    55261 atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt ctttccgtga
    55321 catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa ccgaagacag
    55381 tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa tatattgaac
    55441 agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt ggtcaaacac
    55501 ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa cgatctgagg
    55561 tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag cagattcata
    55621 caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa caccaaaatt
    55681 cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc gaaaaaattg
    55741 agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat tcggatcaag
    55801 caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa tgtaaaatgt
    55861 cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg ctaataaacc
    55921 aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa tcaacgaatt
    55981 aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa aatttcttga
    56041 tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa gcggtatgac
    56101 agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag cacgatcagg
    56161 acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca ttttgataaa
    56221 atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc tgtgattttt
    56281 gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat cttgccaaca
    56341 gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg catcatctgc
    56401 atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca gtggattgat
    56461 gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct ctgcatagtc
    56521 catttgtttc gataattcag atacaatagg atcatttttt acttcttcat cttttgctac
    56581 tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa tttcagttgt
    56641 attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg tacttgccgg
    56701 aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac tcccttgtgg
    56761 taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta accatggccc
    56821 attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg cgccagaaat
    56881 cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta acgttttaag
    56941 gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt taccgccatt
    57001 tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa aacctgatac
    57061 atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc aagtagtagg
    57121 tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat acatatacat
    57181 gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta attcaatagc
    57241 cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt ctaatggttg
    57301 taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa taaagtcagg
    57361 tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc aagtcatgat
    57421 atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa cgatttcttg
    57481 caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca cttctttatc
    57541 agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg ttgcagctac
    57601 caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct tcatctgata
    57661 aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt ttcccatctt
    57721 cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga agattagttg
    57781 cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat gataattcag
    57841 acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat atgacaagtt
    57901 ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa tcttccgtcg
    57961 catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac acagcagctt
    58021 gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct gtcctgattg
    58081 gtgcaataac atttaccaat tgagcaagac aagcaatctt tactctatct gcatgtttca
    58141 ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag ttatcttcta
    58201 gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct gaatgatacc
    58261 aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc ttcgccttga
    58321 ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag gaacgtgcaa
    58381 gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac aaataatcta
    58441 tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta ggcatttgcc
    58501 gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc atcgccttag
    58561 ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc tgccaaggac
    58621 cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct tgaccattcg
    58681 aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg actaaattac
    58741 gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg gcccccacat
    58801 gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt gtccgccaag
    58861 ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc caacgaaatc
    58921 ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct tttaccaagt
    58981 gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc tgataaatcc
    59041 cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc ggatcaactt
    59101 tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac ctcctttaat
    59161 ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg ttaaacttac
    59221 ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca aataatttaa
    59281 tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga ggtgatatat
    59341 atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc tagtaaaaca
    59401 aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga tttggcaaaa
    59461 gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga ggaagcaaac
    59521 atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt cgataaaatc
    59581 gatatcagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac atcgatacct
    59641 attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac gatccatgat
    59701 tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat ggatgcccgc
    59761 attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt aaatacagag
    59821 gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc tttttcaaat
    59881 gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg aacatggacg
    59941 agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta tccagataat
    60001 cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac gaatatgaat
    60061 ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga cacttggcat
    60121 ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt atttggtaaa
    60181 ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg atatttttct
    60241 tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt ttttgcttta
    60301 ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat gttgtgtctt
    60361 gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc cttctagata
    60421 atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca ttggtttccc
    60481 atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat tcaatgcttg
    60541 aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt cggctccacc
    60601 atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg tccctaatac
    60661 ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac gtgctttgat
    60721 atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt gtacttgtgc
    60781 atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa agtcaagagc
    60841 agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag cgataaattg
    60901 atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg ccaaacggcc
    60961 tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa gaaacggata
    61021 ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt ttggatcaat
    61081 tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt attctcctcc
    61141 tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat agccatttta
    61201 tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa gtctcgttta
    61261 tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc gtttagtgca
    61321 ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg gcgagctgta
    61381 aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa gatagcttct
    61441 tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt cccatcaaaa
    61501 tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc tgcattgctt
    61561 gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac tagcttgaat
    61621 actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac accccataca
    61681 tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa gtcattcgaa
    61741 gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac acgcttagat
    61801 atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt gtcacctaat
    61861 tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata agtgtcgact
    61921 aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata atcatttcca
    61981 taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa tatcttccca
    62041 gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt tccccagact
    62101 gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc ttctccgatg
    62161 acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac catattcaac
    62221 agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat taatggttca
    62281 tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa ttcaaaattt
    62341 tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta agcaatatcg
    62401 gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc tgcaaaaacc
    62461 gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc atgtaaatca
    62521 gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc cgtatgcagc
    62581 gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg gcaagttttc
    62641 atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca ttttttctga
    62701 aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc taaaactatc
    62761 ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca atgtatccga
    62821 gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta gattttccta
    62881 ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat gtctctacaa
    62941 cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt gcttcttatc
    63001 cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc gaattatgac
    63061 aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt taaaaagata
    63121 caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca tcaatcagtc
    63181 tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa gatgattatt
    63241 agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat atctttattt
    63301 ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac atggactttg
    63361 acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg
    63421 atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc taccaatagc
    63481 agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt cccaaaaaag
    63541 aattccacta ctttaggata aaatgtctta atatttaaat tactattcac aggtgtttcg
    63601 attagcttca tctcattcct cttttctata tagtcgattt agatccaatc ttaagttatg
    63661 ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag acaaaaggat
    63721 ttacctttta tcacttaata gtataacata atttcgtgag aaaaaaatac aattttttct
    63781 tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa ataatggagt
    63841 acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg cgcttttatg
    63901 atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg atttatggca
    63961 aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca ttcaagttgg
    64021 atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct ttaatcaagc
    64081 atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca actgtccaac
    64141 agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa tttgaagggt
    64201 ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt cgacaaaaat
    64261 acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat aaagaaacgt
    64321 atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt tacttaaaag
    64381 aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag tggttctctt
    64441 acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg tacgaagccg
    64501 atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca aaagctttaa
    64561 atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa gtacacgtaa
    64621 aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa cttttggtgt
    64681 ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc gttgaataaa
    64741 cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa atgccatttc
    64801 tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc tgatcagcaa
    64861 attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca agatgcctgc
    64921 tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa aaaagaagcc
    64981 tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt catcaaaaga
    65041 tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta gcctgccact
    65101 tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag taagaccttc
    65161 tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct tgatttcttc
    65221 acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg tattggcacg
    65281 agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat accttcgctc
    65341 tttttcttta tgatagcccg tcggtaaaag tgtcactttg taatcataat caaacgctgc
    65401 ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag gcagaatcac
    65461 tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag ggatataatc
    65521 gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat atggatgtcc
    65581 attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga tatctcgaat
    65641 gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata gtctaccaca
    65701 gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact gatccgctgc
    65761 ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat ttcttttact
    65821 atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt gaactcgaca
    65881 aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt attcattgca
    65941 agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc aaggagtttg
    66001 ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt attttaaatt
    66061 gaatacatta taatgatact gtaaatatat ctttattatt agtgaatg
//
LOCUS       DACDLI010000007        62935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDLI010000007 DACDLI010000000
VERSION     DACDLI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.007"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..1497
                     /gene="nhaC"
                     /locus_tag="IV353_000486"
     CDS             79..1497
                     /gene="nhaC"
                     /locus_tag="IV353_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9008760.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(2000..2443)
                     /locus_tag="IV353_000487"
     CDS             complement(2000..2443)
                     /locus_tag="IV353_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9008761.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2661..4001)
                     /locus_tag="IV353_000488"
     CDS             complement(2661..4001)
                     /locus_tag="IV353_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9008762.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            4270..5166
                     /locus_tag="IV353_000489"
     CDS             4270..5166
                     /locus_tag="IV353_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9008763.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(5316..6257)
                     /locus_tag="IV353_000490"
     CDS             complement(5316..6257)
                     /locus_tag="IV353_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9008764.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            6383..7504
                     /locus_tag="IV353_000491"
     CDS             6383..7504
                     /locus_tag="IV353_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9008765.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            7762..9264
                     /locus_tag="IV353_000492"
     CDS             7762..9264
                     /locus_tag="IV353_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9008766.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            9331..9858
                     /locus_tag="IV353_000493"
     CDS             9331..9858
                     /locus_tag="IV353_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9008767.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            10069..11130
                     /locus_tag="IV353_000494"
     CDS             10069..11130
                     /locus_tag="IV353_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9008768.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            11133..12029
                     /gene="murQ"
                     /locus_tag="IV353_000495"
     CDS             11133..12029
                     /gene="murQ"
                     /locus_tag="IV353_000495"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9008769.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            12043..14025
                     /locus_tag="IV353_000496"
     CDS             12043..14025
                     /locus_tag="IV353_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9008770.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(14090..14536)
                     /gene="nrdI"
                     /locus_tag="IV353_000497"
     CDS             complement(14090..14536)
                     /gene="nrdI"
                     /locus_tag="IV353_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9008771.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            14747..16171
                     /locus_tag="IV353_000498"
     CDS             14747..16171
                     /locus_tag="IV353_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9008772.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(16352..17125)
                     /locus_tag="IV353_000499"
     CDS             complement(16352..17125)
                     /locus_tag="IV353_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9008773.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(17122..18027)
                     /locus_tag="IV353_000500"
     CDS             complement(17122..18027)
                     /locus_tag="IV353_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9008774.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(18181..18501)
                     /locus_tag="IV353_000501"
     CDS             complement(18181..18501)
                     /locus_tag="IV353_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9008775.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18975..19661
                     /locus_tag="IV353_000502"
     CDS             18975..19661
                     /locus_tag="IV353_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9008776.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            19663..20607
                     /locus_tag="IV353_000503"
     CDS             19663..20607
                     /locus_tag="IV353_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9008777.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            20806..21450
                     /gene="pcp"
                     /locus_tag="IV353_000504"
     CDS             20806..21450
                     /gene="pcp"
                     /locus_tag="IV353_000504"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9008778.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            21529..22806
                     /locus_tag="IV353_000505"
     CDS             21529..22806
                     /locus_tag="IV353_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008779.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(22990..23739)
                     /locus_tag="IV353_000506"
     CDS             complement(22990..23739)
                     /locus_tag="IV353_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9008780.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            24069..24449
                     /locus_tag="IV353_000507"
     CDS             24069..24449
                     /locus_tag="IV353_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9008781.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            24545..25054
                     /locus_tag="IV353_000508"
     CDS             24545..25054
                     /locus_tag="IV353_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008782.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            25131..25310
                     /locus_tag="IV353_000509"
     CDS             25131..25310
                     /locus_tag="IV353_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008783.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            25365..25928
                     /locus_tag="IV353_000510"
     CDS             25365..25928
                     /locus_tag="IV353_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9008784.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(25975..26490)
                     /locus_tag="IV353_000511"
     CDS             complement(25975..26490)
                     /locus_tag="IV353_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008785.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(26516..27385)
                     /locus_tag="IV353_000512"
     CDS             complement(26516..27385)
                     /locus_tag="IV353_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9008786.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            27511..28191
                     /locus_tag="IV353_000513"
     CDS             27511..28191
                     /locus_tag="IV353_000513"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9008787.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            28344..29324
                     /gene="pta"
                     /locus_tag="IV353_000514"
     CDS             28344..29324
                     /gene="pta"
                     /locus_tag="IV353_000514"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9008788.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            29447..29920
                     /gene="tsaE"
                     /locus_tag="IV353_000515"
     CDS             29447..29920
                     /gene="tsaE"
                     /locus_tag="IV353_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9008789.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            29913..30437
                     /locus_tag="IV353_000516"
     CDS             29913..30437
                     /locus_tag="IV353_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9008790.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(30927..31460)
                     /locus_tag="IV353_000517"
     CDS             complement(30927..31460)
                     /locus_tag="IV353_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9008791.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(32446..33198)
                     /gene="xth"
                     /locus_tag="IV353_000518"
     CDS             complement(32446..33198)
                     /gene="xth"
                     /locus_tag="IV353_000518"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9008792.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            33311..34231
                     /locus_tag="IV353_000519"
     CDS             33311..34231
                     /locus_tag="IV353_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9008793.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            34304..35239
                     /gene="murB"
                     /locus_tag="IV353_000520"
     CDS             34304..35239
                     /gene="murB"
                     /locus_tag="IV353_000520"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9008794.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            35537..37015
                     /locus_tag="IV353_000521"
     CDS             35537..37015
                     /locus_tag="IV353_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9008795.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            37292..39352
                     /locus_tag="IV353_000522"
     CDS             37292..39352
                     /locus_tag="IV353_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008796.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            39435..40004
                     /locus_tag="IV353_000523"
     CDS             39435..40004
                     /locus_tag="IV353_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9008797.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(40179..40985)
                     /locus_tag="IV353_000524"
     CDS             complement(40179..40985)
                     /locus_tag="IV353_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008798.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            41123..42637
                     /locus_tag="IV353_000525"
     CDS             41123..42637
                     /locus_tag="IV353_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9008799.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(43040..44023)
                     /locus_tag="IV353_000526"
     CDS             complement(43040..44023)
                     /locus_tag="IV353_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9008800.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMKANKNIGKIILKITE"
     gene            complement(44028..44675)
                     /locus_tag="IV353_000527"
     CDS             complement(44028..44675)
                     /locus_tag="IV353_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9008801.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(44760..45392)
                     /locus_tag="IV353_000528"
     CDS             complement(44760..45392)
                     /locus_tag="IV353_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9008802.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(45408..45959)
                     /gene="coaC"
                     /locus_tag="IV353_000529"
     CDS             complement(45408..45959)
                     /gene="coaC"
                     /locus_tag="IV353_000529"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9008803.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(45956..46693)
                     /locus_tag="IV353_000530"
     CDS             complement(45956..46693)
                     /locus_tag="IV353_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9008804.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEETQSKSEIAALITEKIRLEESK"
     gene            complement(46690..47679)
                     /locus_tag="IV353_000531"
     CDS             complement(46690..47679)
                     /locus_tag="IV353_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9008805.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            47937..48479
                     /locus_tag="IV353_000532"
     CDS             47937..48479
                     /locus_tag="IV353_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9008806.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            48496..49581
                     /locus_tag="IV353_000533"
     CDS             48496..49581
                     /locus_tag="IV353_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008807.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            49593..50399
                     /locus_tag="IV353_000534"
     CDS             49593..50399
                     /locus_tag="IV353_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9008808.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            50400..51227
                     /locus_tag="IV353_000535"
     CDS             50400..51227
                     /locus_tag="IV353_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9008809.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            51224..52297
                     /locus_tag="IV353_000536"
     CDS             51224..52297
                     /locus_tag="IV353_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9008810.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            52725..55421
                     /locus_tag="IV353_000537"
     CDS             52725..55421
                     /locus_tag="IV353_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9008811.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            55498..55986
                     /locus_tag="IV353_000538"
     CDS             55498..55986
                     /locus_tag="IV353_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9008812.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            55998..56492
                     /locus_tag="IV353_000539"
     CDS             55998..56492
                     /locus_tag="IV353_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9008813.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            56513..57322
                     /locus_tag="IV353_000540"
     CDS             56513..57322
                     /locus_tag="IV353_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9008814.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            57319..58140
                     /locus_tag="IV353_000541"
     CDS             57319..58140
                     /locus_tag="IV353_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9008815.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            58191..60290
                     /locus_tag="IV353_000542"
     CDS             58191..60290
                     /locus_tag="IV353_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9008816.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            60287..61405
                     /locus_tag="IV353_000543"
     CDS             60287..61405
                     /locus_tag="IV353_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9008817.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            61444..62322
                     /locus_tag="IV353_000544"
     CDS             61444..62322
                     /locus_tag="IV353_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9008818.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            62513..62665
                     /locus_tag="IV353_000545"
     CDS             62513..62665
                     /locus_tag="IV353_000545"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008819.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        20287 a        11064 c        13020 g        18564 t
ORIGIN      
        1 gcggatgaat tgcggcggag taatccgata tcttaacttt tttagaacat aagaacaact
       61 aaaaaaggag agattatcat gggaaaaaaa ggaacaccaa gttttttaga agcaacactt
      121 gtattaatgg ttattgtttt aggcattgcg attggcgttg tcggattaaa ggtctcacct
      181 aatatcacga tcttagcagc gatcggaatg gtcatgttgt atgcagtgat aaaaagatac
      241 ccgacagatt ggctgcacga aggaatcatc aatggcatca aaccaggaat catcccaatc
      301 tttattttta ttttagtcgg ttcattgatt gctgtatgga tacaagcagg gatcattcca
      361 actctaatgg tcattgggtt caaattgatc agtgtccagt ggtttgtccc ttctgtattt
      421 gttgtgtgtg caatcgtagg tagcgctgta gggagtgcct ttactgttat gtcaacgatt
      481 gggattgcat ttttcggtat tgggacaaca ctaggcttaa atccagcgct ggttgttgga
      541 gctattgttt caggtgccgt ttttggagat aagatgtctc cattatccga atcaacaaat
      601 ctagcagcag ctattgttga tgccgatttg ttcaaacaca tcaagcatat gatgtggtca
      661 actgtcccgg catttgttgt ttctttcttt ttattcatga ttttaggaca cacaagtaaa
      721 gccgcaagtc tttcatctat tcgtgaagtg actgatgttc tagaagcaaa ctttactatt
      781 tcaatctggt cactgattcc tttattttta atgttactgt gtgcatggaa aaaggtgccg
      841 gcaattttaa cgattttgtt gaatatcgct gtagcagtag gaatgatttt tattcaaaac
      901 ccacaaactt ccttaacgaa gttggctggg gtcatcgaat cagggtttgt ttcaaatact
      961 ggaaaccaac aaatcgattc actgcttagc cgcggaggaa tcgaaagtat gatgcctact
     1021 gtgtcattga ttattctaac tttgtcatta ggtggattgt tgatcgaatt tggcttgatc
     1081 tcaaccgtga tggaacgagt atcaagaaag atgacgaaca caccaaattt gatttttaca
     1141 acattgatga caagtatcgg agtaaatatt tttattggtg aacaattcct atctgttatt
     1201 ctgcctggta atgcatttaa agaaacttat gaaaaagcag gatttgatcc aactgtatta
     1261 ggtagaacgc tagaagatgg tggaacagta atcaactact tagtgccttg gggaattgcc
     1321 ggaagtttcg tagcaagtac atttggtatt cctacactca cttacttgcc attcgtattt
     1381 ttcagtttgc tttctcctgt gttttcgatg gtcagtgcct tcacaggttg gggaatctca
     1441 aaaaaatttg tgaaaccgca agaagcagta agcaagacag cgcaattaga acaataaaaa
     1501 cataaaaagg gattgtgaca agacttttgt cacaatccct tttacgaata aacggtattc
     1561 aaaagccagt ttttcggtat taagccaaat ttatacaaaa atgtggaaag ccatttcaca
     1621 aaatttttct taatacgcga aactaagcga ctttttcaca acctctcgat aaacggtgtt
     1681 caatcagctg accctcggta ttagctcaaa aaatggcaaa atatgaggaa ctatttttcc
     1741 atttgtctct ctaatactca ggtcagaacg ctgattttac aacctcttaa taaacggtgt
     1801 tcaaaagctt gcatctgcgg taataagcct aaccaaacga aaaataggag aagctatttt
     1861 tgtttggctg ttcttattac ttgatgcagg cagctttttc acaacccctt attcacggcg
     1921 ttccagaagt agcttcttct tatttcttga agctgataac ttctggaaca tcctcttttt
     1981 ttatttggac ggataatggt cattccattt ctttgatcat ttttttcatt ttgtcgatct
     2041 cttcttcttg tgcctgaatg atttcttccg ctaatttttt tacttcagga tcttttaaat
     2101 cagctcgttt acttgccagg atagcgatag aatgatgagg aatcatggcc ttcatccaag
     2161 ctgtgtcgtt gacagtagtt tggcttcgta ccataaacaa ggaaccggca aaaacaagaa
     2221 gactagcggc taagatcata acatttatct ttttgttttc atacattttc cacatgaaca
     2281 acagcatgat gactgccatg actgcaccca tcatcaacgc catgaatact cgtgtacgac
     2341 tgaagaagat atgatcgagc gcatagacat tcaagtacat cataccaaac ataactaccg
     2401 ttgaaacacc gatcatcaac aaaaacttcc aatacttttt catttaaaac aaactccttt
     2461 ttttcattcc tttcttctat catgtcatga gcatggagaa caatcaatta ttttgcacag
     2521 agggtttttg aacaccgttt accaagaggt catgagactg tttattcgga aaaaggagtt
     2581 gcaacaagac ttttgtcaca actcctttct tcaatagctc catctattcc acataaataa
     2641 aataaaattt tctatcgatt tcattcattc gtcaatacga acgcatcatt ctttttttga
     2701 taactgaaat aaatgaacag gtaaagcagg ccagtcaata cagttacccc aatggagttc
     2761 gtgaacagga taccaaggaa aatcaatcct aagacagcta atgcaaaaaa gctgattcct
     2821 ttatcccata atgttccttt tcttagtgcc agtacaaagg atgccgcact gataatgatc
     2881 cagatcaata atactgtata cgaaagggaa cctgccagat agccaaataa tttgtcaccg
     2941 acaaaataag aaagagctac accaagataa agaacactgg tacaaaataa aacagcacgt
     3001 tgcggtactt gatgcttgtt taaaacagct agttttctag aggaaccttt tcgatccttc
     3061 aaacggaaga acaaaattct tgaagatgca tacacacccg aattgataga tgagaacaaa
     3121 gcaaggacaa tcacaaaatt gacgatatcc gctgcaaatg ggatattcat tttttcaaat
     3181 accatcacaa aaggactgac attcgtgcct gctaaagtat tccaaggaaa aagaatcaaa
     3241 agcaaaaaca aaggaatgat gtaaaaggaa atgattcttc ccatgacacc tttgattgct
     3301 ttaggaatcg cacgtttagg atcatctgct tcgcttaccg tgatagcaat cagttcagaa
     3361 ccgccgtaag aatagataac tacaagcaaa gaactgatga ttcccttcat accatgagga
     3421 gtaaacccac catggtcagt caattgctga agggtaggga aaacgcctgt gcccaacatt
     3481 tgctgaccga caagataagt agcaaaaatg atcagtaaga taatgacact gattttagca
     3541 aaagccagcc aatattctgt ttctgcgaat aaccgtaccg aataaagatt gatgatcgta
     3601 gttaaaacag ccaacataaa aacaaatacc caggctggaa tgtgcgggaa ccacaactgc
     3661 aaaaaggaag cagctgcaac agcttcagcg atgatattga taatccacat agaccagtag
     3721 acccaatcag taaaatcagc agaatgagca cctagataag ggcgtatcaa accagataat
     3781 ccgtgatgcg tgtcgccacc gcttaaaacg atttttccca gacctttcat cacgatatat
     3841 aaaatcagcc caccaatcaa aaaagcaatc aatacagaag ggccggcgat cccaatcgct
     3901 tcgccacttc ctttaaatag acctgcaccg attgctccac ctaaagctaa catggtgata
     3961 tgtctggagc taagttgttt tttcaatttt ttatcttcca taaatgacct ctttctttta
     4021 aattcacaat caaaatttat attaagaata aatagcaata tttaacaatg actaatgata
     4081 acaaattttc agacaattac aactattcag aaaactttta ggcgaatttt aatagaagaa
     4141 tgatttattt catcatattt tcatacaaat gttaaatttt catacaaata taggaaacgt
     4201 tttactggct ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag
     4261 gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc actttatttt
     4321 ttgccttcca tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc
     4381 attaaatcag ctagacaaca gttggcaaat tatcagctga tcgttttttt agattatgga
     4441 tttgatatgg agatggctcg acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt
     4501 ttttggaatc acttcaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg
     4561 gcaatcgatg agatttatca ttttgatttt ttggaagcaa aagaacttgg gctgaaacat
     4621 aatagttcat tttattcgaa acatatgaat ttttcttcta gcgaaccaaa gacggatcta
     4681 ttttttggtg caacgaataa tgggagaaaa gaacgagcag aaagctataa gcaggaattt
     4741 ataaaaagag atgttacgac caactatttc attcttccac atcgagggaa tgaacaacct
     4801 ggatatctta cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa
     4861 ctgttgcggg agggtcagca aggagtgact ttgcggacat ttgaatccat gtattttcaa
     4921 aagaaactag tgacggataa tcaagcgatt gctcattatc atttttatcg tcctgaaaat
     4981 atttttttgc ttcgagaacg tgatttagat gaattaccgg aatttctgca tacaccattt
     5041 aagccaatcc tatcagagct attggaattt tatgatgcag aaaattgggc aaaacgattt
     5101 atcactaacg attccactat ttatgagcaa tacgaatatg acctctcctc tgttgaaaac
     5161 aaataaaaaa cctacatttt gaagagatta tgtactgaaa caaacaagca caattcttat
     5221 ggcatcatga gagttgtgtt ttctttttga tcttttattg ggcttaaaaa aagagaacca
     5281 gctgctgatt cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct
     5341 tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt
     5401 ccttttcgcc aaatggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc
     5461 aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca
     5521 atatgttggt acacttcttc ttgatctagt atgattcctt cttttgctgc gatagaagca
     5581 aattcagata cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc tagttcagca
     5641 atattacaat ccaaaatcgt gcataaacca ttcaatgttc catttacaca agcttttcgc
     5701 caaatggaat aacgaacgtt atggctatag atcggattta aatttgcttc ttgaaagact
     5761 tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct
     5821 ccatcaccta acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc
     5881 ccaaataaga tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca
     5941 tttagtaaac acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata
     6001 tcatctaatt gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg
     6061 atgatttctt ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca
     6121 gcaatcaacc catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg
     6181 acctcatttc cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca
     6241 gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata
     6301 gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt
     6361 aagaagaaag tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag
     6421 tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag
     6481 aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc
     6541 gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat
     6601 cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc
     6661 attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta
     6721 ttccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac
     6781 tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc
     6841 tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg
     6901 aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata
     6961 tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac
     7021 gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga
     7081 ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt
     7141 tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga
     7201 aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac
     7261 gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat
     7321 aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat
     7381 tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac
     7441 attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa
     7501 gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg
     7561 gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg
     7621 gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc
     7681 tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct
     7741 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
     7801 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
     7861 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
     7921 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
     7981 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
     8041 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
     8101 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
     8161 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
     8221 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
     8281 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
     8341 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
     8401 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
     8461 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
     8521 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
     8581 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
     8641 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
     8701 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
     8761 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
     8821 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag
     8881 ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg
     8941 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
     9001 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
     9061 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
     9121 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
     9181 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
     9241 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
     9301 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     9361 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
     9421 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     9481 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
     9541 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
     9601 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
     9661 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
     9721 gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat
     9781 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
     9841 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
     9901 cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac
     9961 ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat
    10021 atactctagt catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt
    10081 tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca
    10141 tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca
    10201 tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg
    10261 gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct
    10321 ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt
    10381 gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat
    10441 atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat
    10501 tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc
    10561 aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca
    10621 ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct
    10681 ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa
    10741 aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa
    10801 cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat
    10861 gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca
    10921 agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc
    10981 tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta
    11041 gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac
    11101 tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa
    11161 caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg
    11221 ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa
    11281 gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt
    11341 atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga
    11401 cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga
    11461 ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc
    11521 tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg
    11581 tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca
    11641 ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag
    11701 aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata
    11761 tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg
    11821 tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag
    11881 aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg
    11941 ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag
    12001 caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg
    12061 actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa
    12121 agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat
    12181 gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa
    12241 gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc
    12301 gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct
    12361 ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa
    12421 aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt
    12481 cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc
    12541 acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg
    12601 aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt
    12661 ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat
    12721 ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg
    12781 atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa
    12841 gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcattgat tgccatcggt
    12901 ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt
    12961 gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta
    13021 ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg
    13081 attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa
    13141 gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg
    13201 atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta
    13261 acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca
    13321 gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg
    13381 atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat
    13441 gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa
    13501 gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact
    13561 tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat
    13621 aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca
    13681 gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa
    13741 cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac
    13801 acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca
    13861 cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca
    13921 tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca
    13981 ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat
    14041 agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt
    14101 tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat
    14161 ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat
    14221 tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat
    14281 gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat
    14341 ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat
    14401 cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca
    14461 ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata
    14521 tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc atctttttta
    14581 agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa
    14641 agccggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt
    14701 tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca
    14761 acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg
    14821 aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt
    14881 cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa
    14941 taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg
    15001 tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc
    15061 ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca
    15121 aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa
    15181 tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc
    15241 attattttta tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa
    15301 cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt
    15361 attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt
    15421 aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg
    15481 cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca
    15541 gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa
    15601 atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc
    15661 atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa
    15721 agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt
    15781 atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata actttgctta
    15841 cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt accaaatgaa
    15901 actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag caagtgcctt
    15961 tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc ttgttctttt
    16021 cttgccaggc atttattttt acctcaaagc acgtaaggag caaaacgagc ctgtacaaca
    16081 tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga ttgcaatggt
    16141 tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa agagaggttg
    16201 tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa atgttgtcac
    16261 agctcctttt tcagaataaa cggtgtttaa gagacagcca atcggcaata aggctgaacg
    16321 tctctttcac agcctctttt ttgatgagtc atcatacatt ttttcgctta aatacgagat
    16381 atcctaatgc taagaacaaa acgatgtaag ctaaattacc gaatagcatt tgattagtag
    16441 aaagatgagt caattgcttc atgatctctt cgtttccaat ttggttttgc agattcaaca
    16501 tactgatcgg attccatttg atccactccc atttttggat gagtgcaata aggataccag
    16561 atatgattga cgaagcaaaa taaaagacga ttcctgctga gatggatgca ccggaactat
    16621 tgataaagca agcaatcatc aacactaaac ttaaaatcag ccataaaccg atataactac
    16681 ccaaagtata agtgaacaag ctttggatca gtgtttgacc agtagaaacc ttctccgtga
    16741 aggaaatcga agggaaaaga atcagtttca tcaacacagt gacaataata gctaaaaggt
    16801 ataaataaac agaataaata actaatgtga tccatttgct tactaatatt tctcctcgag
    16861 aatactttcg ataaaggaga tttttcaatg taccatattg tgcttccatc gaaatgatcg
    16921 tgcttgcagc tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa agctgagcag
    16981 tcatttcaac tggatcaatg atcgtcgtgt attttttggc tagaagtgct gttcctatga
    17041 gcaaaacaac taatagaaca gataaaacag cagttgactt tttcttgatc aatttaaata
    17101 attcttgttt tacaagcaat gtcatttttc ttcttccccc tttactgaat ccagaatttc
    17161 caatagagaa gattccaaat cattcgtcag atgctggacg tctgtcacat ggatattttg
    17221 tgcagacaat agctggatca cggttcctaa taattcttcg ttttctttta aaagcttgat
    17281 tttatcagga tcttccaaag tatatccagc ttctgtcaag atcgttcgag cagctagatc
    17341 atttgttgta gagataacga tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca
    17401 ttgtttgatg atctttcctt gattgatcag gatcagctca tcgaccattt tttcaagctc
    17461 gctgagtaag tgactggaga tcaagaaaga ggtgccctga gtagccaagt cggtaatgat
    17521 ttttcgcaca tctcttgttg cttgtggatc caaaccgttc atcggctcat ctaaaataac
    17581 gatagacgga tggttcagta aggcaagagc aattcccaat ttttgtttca ttcccaacga
    17641 gtaggatttc gcttttttgt gaatgtactt ttccatcttg agctgattca cgatagtgtc
    17701 gatagcagat acatcgttgg tttcagagaa taacttcaga tgatcatagc ctgttaagaa
    17761 tggatagata cccgggtatt cgatcaatgc tcctacttgt tccaaccctt tatgactaga
    17821 gggagaaatc tcattttcac caatacagat tgttccatct gagtaatgga tcagtcctaa
    17881 aatagctttc atgatcgttg tttttccagc gccattagga cctactaatc caacgatttt
    17941 gccaggggat aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa agttcttgga
    18001 taattcttgg accgaaagac ttttcaaaaa tattcactcc ttttctatag atagtacaag
    18061 catacacctg atgagtggag aagtcgctag gtcattggac ttatttctta ttttttcata
    18121 aaaaaattga atattaaata aaaaggctta ttttttctga tcagtactta ttccaagtgt
    18181 ctagtggccg ctagtaatct tgatgccaat gatactgatg agtagcatcc ccacaaagaa
    18241 ccaagtggaa agattcagct tatcatgaaa caagaccaca ccaattatga ccgaaccaac
    18301 tgcgccaatc cctgtccaga taggataaga gagactcatc ggcaaacttt tgattgcctt
    18361 tgacaaaaag taaaaactag caatcatgcc aatcactgtg taaaaagaat aattgatttt
    18421 agaaaaacca tcactccatt tcatcatcgt tgaccagaat acttccaaaa ttcctgctat
    18481 gattaattcc atccatgcca tacttacatt cctccttaac tataaaaaaa tacacaaaaa
    18541 gaggaaaatc atcccttcaa agacctaatg gaatgatcat cctctttatc ctacccggtg
    18601 gcagattgta tatctttttc tgtagtatag cacacctatc cagatatcgg caatctcata
    18661 ctttttagta aagtgtacca agcactgacg gatatatcgt tagcggaaaa gaagatgaat
    18721 gctttgcaat ttcatagaaa gaagttgtta tttattaatc atttaaaaaa aacagtaact
    18781 agaccttatt tctatatatt atcgggcaat aaattaaaaa aagctttacc ttacaataag
    18841 taagataatt tttgtgataa aaaaataacg aaaaatattt aaaaatagtg tctatttcgt
    18901 gagaaaacaa ctatattttt tatgtgtttt gctttataat taaaaaaata ttgtcatttt
    18961 atggaggaaa atatatggaa aattatctta gccttttagg cgtcttgatt gttattctcg
    19021 ggtttgcatt taaattagac tcgatattga tcgtaatggt tgctttagtt gttacagctt
    19081 tgactggcgg tcttggcatt caaggaatgt tagaactttt aggtacaagt tttgtaaaca
    19141 atcgcgggat ggcgattttt atcatcatta tgttggcaac aggaacgctt gaaagaaatg
    19201 gtctgaaaga atcagcggct tccttgatca agcgattcaa gaaagtatct gctggtatga
    19261 ttgtagatat ttatgggatc tttcgaatga tctttgcggc ttttaacgtg agttttggtg
    19321 gagttgctgg atttgttcgt ccaatcattt taccgatggc attggggacc atcgaatcaa
    19381 aagggttaga agtccatcct aagcatgaag aagaactaaa aggtatggct tcagctatgg
    19441 aaaatgtctg ctggttcttt ggccaagtac tattcatcgg aggagcaggg gcgttgctag
    19501 tccaatcaac actgaaagat ctaggatatg acgtaacatt gggacagctt gctttagtgg
    19561 aagttccagt ggctatcacg gctttagttg tggcaagtgt ctacttctat cttaaagaaa
    19621 agaaattagc gaagaaatat tatccaggaa aggggcaata agatggactt ttttacaaat
    19681 cctgatattt tattaggcga taaattactg gagatccttt atatcgtgat gggactcgta
    19741 ttgatttaca ctggagtaag aaatttaaca gataaaacca atcctcatcg ttatgggtca
    19801 gcctttttct ggacggtctt aggggtagtt attgcaggag gaagatggct tccggcaatc
    19861 gtgaacggtg gcttgatctt tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa
    19921 ggagaaagcc ggcttccatc aaaagaatat atggaaaaaa tgtcagacaa actaggaatg
    19981 aaaattttta ttccagcttt gagtatcggt gtctttgcga tcttgtttgc tctatttacc
    20041 aatttaggtg ctttagtcgg cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg
    20101 ttcttttctc gagataatcg accaagtaca tttttagatg atgcagcaga tatgctgggc
    20161 acagtagggc cgttaagcat gttgccaatg cttttagctt ctctaggtgc ggtatttaca
    20221 agcgctggag ttgggacagt gatttccaat gcagtaggta cgatcgttcc agaaggaaat
    20281 gtgaacatcg gcatcatcat ttacgctctt ggcatggtga tatttacggt aatcatgggg
    20341 aatgcatttg cggccatcac cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt
    20401 catggagcga atccagcgtt aataggaatg gttgcattga cttgcggttt ttgtgggacc
    20461 ctgctgacac cgatggctgc taacttcaat atcgtgcctg tagcgatgct ggagatgaaa
    20521 gacaaatacg gagtgatcaa aaaccagttg tttattgcgt tatttatgct agttttccaa
    20581 attgcttata tgattctatt taaataatag aagagagggg ttgtgacaga ataattgtcg
    20641 caacctcttc taacattgta gtggaacact atttttttat aagaatatat taagtttgat
    20701 ctgttatgtt atttaaacta ttgtttcctt tatttacgct gttcaaggaa aatggtatga
    20761 taaaagataa ccatatgtta aatcaatcgg acggaggaac aacagatgaa agtactagta
    20821 acaggttttg atccatttgg tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa
    20881 atgccagatg aaatcagcgg tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa
    20941 aaaagcagtc aagtagtcaa agaagccatc caacagcacc aaccagatgt cgtgatctgt
    21001 gtcggacaag caggtgggcg gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca
    21061 gaagcaagaa tccctgataa tgaaggaaat cagccattcg atacggcttt agaagaaaac
    21121 ggtccagccg cttactttac ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat
    21181 catggtctac cagcgtacat ctcttataca gcaggaacat ttgtctgcaa tgatatcatg
    21241 tatcgtttgc ttcatttgat cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac
    21301 gtgccattct cacccgatca agtgatcgat cgtccagtgg gtacgccttc tatgtcttta
    21361 gaagacattg cttcttcttt gacttatgca gttgaagcag cagtagaaaa taaagaagat
    21421 atttccggaa atgccggaac aactcactaa tttagcaaga aacactggct ggttgctaac
    21481 gttatgttag tagccagctt tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg
    21541 caatccgtct ttgaagaatg gtttccaaaa gaaatcagca aaagctatcc catcaaaatt
    21601 tctaaacaat acacaagcag tcaacgttgg gagatctatg caaaattaac gaaaaaacag
    21661 cgggaactag tcgataagca ccgccgatat ctaatcagct ctcgttttat ggaagaacat
    21721 tatttagctg caacagattg ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca
    21781 aaacgaagcc agcaaaaatt gtattgtgag tgtggtagag aattaaaagt gcaatacatc
    21841 gttaaatcgc caaaaaccgg aaagatccta aaactaggca tcaaccactt tgccgatcac
    21901 ctgcatgtct ctccgacagt ggcagcttcg atccatcagg gaatgacaaa agttgatctg
    21961 gccttagacg aattattatg gcttaagcag aagaatatcg attttcctga aggactatgg
    22021 caaaaatact gttttgtctt gtaccaaaat cgtcggatga aacagccata tttaccggat
    22081 ataaaattag ctcagcggct cgcagaattt cgccaagtag aaatgccgat ttacatagcg
    22141 gattatcaag cattggaaaa tgaaatcaag aaaataagtg aacacatcaa tgggcagtca
    22201 aaaaagcgac agataaaaaa agaactattt gacgactttg cagaagaatt agtaaaggat
    22261 gtagaagaat tcttgatcaa ctatcgcgca tttttacgta aagattggca atccatcgta
    22321 tatgaagaag tgccagtaca tcctaatgct tattttgaaa catttatttc cgtgttgaga
    22381 aagacaaaac gtcaacgaac accagaagtc actgctcaga tggaatattt tgcgaaaaac
    22441 caacgattca tccaaccaaa aatctatcta tttatttgga agcaatattg tcgctatggt
    22501 tttacagaag ggttcttcga cagcattcct cggatagtca gaaatggttt tttaaaagta
    22561 ttgcgtaaag aacgagaagc tatacaatct gctgataaga aagaccgtac agtctcaaaa
    22621 gaaaaatggc aactagtagt gaaagatatc caatctggaa atgtgcaaga gacaatcgac
    22681 aaatggaaag gaaaacatta tcgttttact gaagctcaga aacaagcgtt ggaatattat
    22741 cagaagttag aagaatcact gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta
    22801 ctataaaaaa tagaggctgg gacagaagcg tttaactcca agaaataaga agaaattcac
    22861 gaaaattgct tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgat
    22921 tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc
    22981 tcagtttttt tatgcgtgtt gttgtgcgtt gtagtattct tcttccattt tttgcatacg
    23041 ttcacgttct ttttggttat accaaggtga aacggtaaat gcaaggacat cattcagcaa
    23101 gtaaacagag ctgttcacga acatcgccaa acttgcagat ccatgcgtaa atgaaatata
    23161 ccaaaggacc atttgagcaa gccctgaagc taaccaccag taatattggt tgttgtaacg
    23221 caagaaactg atgataccag cagtcaagct aatagaaaaa gcaatggcat ctacccacgg
    23281 ccgaggatca tctgtataac gaccaatcag gaatcctgaa acaagataga caaggaaagt
    23341 actgatgatt gcgataaacc agctttttcc agtgaatttt cggatttttg aagccatatt
    23401 atgattccat tctttactta acaatacggg gatatccaat gtgatcatat aagcaatctg
    23461 ttcaccgata cttaaatagt tctttgctga aaacccaacg ataataaaac aaatcgcaga
    23521 caaaatacct aaaataccgt tgactgattt tgccgcattg atggataaaa tacacaatac
    23581 accaagcgtg gtaccgataa atgtaataac ggacagccaa gtgatgggac tattgacaag
    23641 agtcatgacc tggcagccaa gactgaaaaa gaaaagataa taattttgtt gcggccagcc
    23701 ttttagctga tgagctaaaa actgaaaata tgacttcatg tatgaggact ccttaagaca
    23761 ctaaatgtag tgtgttctgt atttactaac acaatctatt gtatgctttt cttgaaaaaa
    23821 aactagtctt tcaattataa ataatttgtg ttatgataat tttctgtttg ttttcttgaa
    23881 aatataattt cttactttca tttattatat tgggaacagg aagagtgtct tggaggacct
    23941 actttctgtt ttttttgcct tttttcttat aaaaaggaaa aacagctatt tttttcaatc
    24001 tgacacattt acaatggaac tggagattaa tatagatccg agcttaaacg aagaatagga
    24061 gtgctcatat gaaccttaca acgatccatc atatagccat cattgtttca gattatcaga
    24121 aatcacgtga tttttatgtt aataaattag gatttgaagt aatcagagaa aattaccgca
    24181 aagaacgaga tgactataaa ttagatctga aactaggaag tgctgaattg gagatttttg
    24241 gaattgctaa tgcgccacaa cgaccaaact atccagaagc ttgtggcttg cgtcatcttg
    24301 ctttcaaagt agacgatatt gaagaaacaa ttgcagaatt gcagtcctta gggatcgaat
    24361 cagaaccgat tagagaagat acctttacag gaaaaaaaat gacgtttttc tttgatccag
    24421 acggattgcc attagaactg cacgaataaa gataacaaaa ttgtcacttt tttttcgttt
    24481 cattcgattt ttcattcgtt catttcttga tattctttaa aaaaaagaaa caggagaatg
    24541 aagaatgaaa aaaatgatgt ttggtgttgc tgccctcatc gtaataggga ttaccggtat
    24601 gacgggactg ctcaatgccg cttttcacat ggatgatatt agtgaccagg aatatcggat
    24661 ctcattgaaa aaagcagcag caatcgtaaa aaacgaaaca aaagaacata acatatctgc
    24721 tattcatttt gcagttgaaa aagatttgga gaatcctacg aatgcaacat atgaatacca
    24781 gtttttttgt gaaggacaag tcatagcagt caatccatcc actgggaaaa caaccgtacg
    24841 aaatgacacg gaaaaggccg cagaacatct ttttgatata gaaaaaacgg aagagttcaa
    24901 acaaccgcaa gcagcgatga aggaagcagt cagctactac gggaaaaaag caaaagtgaa
    24961 agagtggaca cttctagtca aagatgaacg tccttattat aaagtccgat taatagatgg
    25021 agaacaggac cgtcaggtat ggatatctgc atagaagaag gatattgctt ctctttttta
    25081 caatataaga gtaagctaag agagtaaaga tgaaaggaag ttgagataac atggaaaaaa
    25141 tgaaatgccc taattgcgga aaaaaatttg catacgaaga agtcaataat gtagtcgaac
    25201 ataatgataa agaaatgcca gtcgtctgcc cttattgccg aactgaagcg gcaagaatcg
    25261 ttactcatgg ttatttcatt actgaaaaga ttgaagattt tttaaaataa cgattttgcg
    25321 atttcttttt ctgcatgtta gaatggagaa aaggagagga aactatgatt cgttttgcga
    25381 gaaaagaaga tgctccagaa atcgccccat tgatcttagt gattctaaaa gatatggaac
    25441 tgccattttt gaagcagaca ggggaacaaa agactttaga gattttagaa gaagcaatca
    25501 ctgatccaaa ttatcgttac agttattcac gaggtatcgt agaagaaata gacggtaaag
    25561 ttgcaggagt tgcttacggt tatacagatg aagaagaatc aatcatcgat cagccactgg
    25621 tacctatctt agagaaatat cagctagatg cgtctacacg tttgttcatt gataaagaaa
    25681 cgtttcccaa cgaatggtat ttggactcta tcagtgtatc agaagatttt agaggacaag
    25741 gcatcggttc gcgattatta gaagcattac ctaaattggc gaagaaagct aatcgctccg
    25801 tcatcggtct aagtgtggat gaaaaaaatc caaaggctaa aaaattatac gaacgccatg
    25861 acttcaaagt tgtcggtcag cggatgatca gtggtcatct ttatgatcat atgcaaaaga
    25921 atatctaaaa agatgtacct gaaatgcgta tttccgcatt tcaggtacat ctttttaact
    25981 taagttaagt cggtaagaaa caggcttatc gcgacttttg tctttacggt agagccagta
    26041 atctaaaacc gcagcaatac aaatacaaac tggagcatct tcatcatttg agacatccaa
    26101 tacatagtaa tctcctgaaa aaagtgttgc tttatccatt ttcatgattt gctgattgaa
    26161 atgatggatt tgatattgat gattatagat atcaccttgg actgtccagt gaagtctacg
    26221 aatataataa aagtcaccgg gccaattgaa tattttctgc atggttccca ccttttcgaa
    26281 ctctttatac agctcaaaac gcgtaccgaa tgtcaggctt atctgcttga tgtgagcaac
    26341 gagatcgcca ttcatggcat aaagagacaa tgcatcgccc tttgttcccc agcgaccaac
    26401 catcaaaaac agggaatgtc ctttttcgtt tttgacaatt gtccgagttg cacgtccaag
    26461 ctgcttttcc tgaatgaaaa attctgacat ctatatccct cttttctaga taaaactaca
    26521 agtcttctgc aatcgcgcgc aatatggctt ttcctacgcc agattcaagg tttgtgtccg
    26581 ttgtatattt ggcataatct tttacttcga tttctgcatt tcccattgcg aaactgactc
    26641 ctgctacttg cagcatgctg acatcattga agttatcgcc aatcgtcatg acttcttcta
    26701 aagaaatccc acgctcgtga gcgacatggg caacagcaat tcctttttga gcgtttttat
    26761 ggttcacttc gatattgttt tgaccagagg aagtaacggc tagatcgcct aaaagagaaa
    26821 tctgctttcc aactggacct aatacagctg gcccttctgt atgaaaacaa ataattttta
    26881 aaacttccag attcttttct tccaaaatag cacgaatact gtccacgtat gtgatgtgca
    26941 ataactccag cttagctgag gccatggcaa tcgccatttt atacgtaagg tgaggtaaat
    27001 gggtagccac catggaagca aaactttcaa ttcgtttcgc ttgattttca gaataaagac
    27061 catcgttggt tgctacttca tagtaaatac cgttagcatc tagaatatct aaaacagaga
    27121 ttgcttgatt atttggaatc gacacggtaa acaatgaatt tccttcttta tcaaatacct
    27181 gtgccccgtt caaagtgatc atcgcacaat cgattcctac ctcatctaaa gctgcgcgag
    27241 cttcttgata gtttcgacca gtagcgacca tgaactcaac accggcatca tttgcaaaac
    27301 gtatcgcctc ggcattttca ggagaaacac tcatatgggc atctaataat gtgccgtcca
    27361 tatctgatgc aattaatttg atcataatga ttatcgttcc tttctgcttc ttgttcctag
    27421 tttaccatat taatcaagtg ctgagggtga agattgtccc ttgtgattga agaaaatgtg
    27481 agttattctt tttattagga ggtggagttt ttgaaagaaa tcattcataa cagttggcaa
    27541 gaagttttgt cctcagaatt ttcaaaagat tattatctgc atctaagaga atttttaaaa
    27601 aaagaatatg cgtcccaaaa gatccatcca gatatgtatc atatctatga agcattggaa
    27661 ttgactcctt atgaagaagt taaagtggtg attttaggac aagatcctta tcatggagaa
    27721 aatcaagctc atggcttatc tttttctgtg caacctggcg taaagatccc gccatcgtta
    27781 agaaatatct ataaagagct ttatgatgat cttggaatcg cacctgttca acatggaaat
    27841 ctagtgtcat gggctaaaca gggagtcctt ttactcaaca ccgttcttac cgttcgggaa
    27901 gggcaagcgt attctcatcg cgggaaaggt tgggaaagat taacagacac gatcattgaa
    27961 aaattaaatg aacgggaaaa accaatcgtg tttatcttgt ggggaaaacc ggcacaagaa
    28021 aaaatcaaaa tgatcgataa gagtcgccat atcatcataa catctccaca tcccagtccg
    28081 ttatctgcaa gtcgtggctt ttttggttcc aaacccttct cgaaaacgaa tgatgcgctc
    28141 cttgcgttag gagaagaacc gattgattgg caactgcctg aaacagtgta ataaaacagc
    28201 gctttcctgt tatagtgcag ttcgctttgt atgtgaaaaa cttaattttt ccagcatttt
    28261 tgactgatac agataaaata aactgttctt tttttctgga atagtgtatg ataatacata
    28321 taaaaacgtc tggaggtcat cttgtggaat tgtttgatag tttaagattt aaaattgttc
    28381 gccgtgggat caaaatcgtt ttcccggaag ctactgatgc tcgtatttta ggcgcagctg
    28441 cccggttgaa agcagaagaa cttgtggaac ccattttagt agggaacgaa gaagaaataa
    28501 gaaatgcagc gcatgccaga ggaatcaaaa catctagctt tacgatcatc agccctgata
    28561 attacgataa gtgggatgaa atggtggata cttttgttga acgtagaaat ggaaaggcat
    28621 ccaaagaaca agcagaaaag attttaaaag atgtgaatta ctttggtacg atgcttacgt
    28681 atatggggat tgcagacgga atggtctctg gagcgatcca ttcgactggc gacactgttc
    28741 gcccagcact tcagatcatc aaaacaaagc ctggcatcag tcggacaagc ggagcgatga
    28801 tcatggtacg tggaagagat caagaaaaat atgtcttttc ggattgtgcg atcaacgtga
    28861 atccaactgc gcaagaattg gctgaaatcg ccgtagattc tgcaaaaaca gcagaattgt
    28921 tcgatatcga accaaaagta gcaatgatga gcttctcgac aaaaggctca gcgaaatcgc
    28981 cagaagtaga caaagtagtt gaagcaacta aaatcgctaa aaaactagcg ccacaataca
    29041 tgatcgacgg cgaattgcaa tttgatgctt cttatgttcc ttcagttgca caactgaaag
    29101 cgccagattc agaagtagct ggtcaagcaa cagtctttgt atttccagaa ttgcagtcag
    29161 gaaatatcgg ctataaaatt gctcagcgtt tcggtaactt tgaagcaatc ggaccaattc
    29221 tacaaggctt gaacaaaccg gtttctgatt tgtctcgtgg agcaaatgaa gaagatgtat
    29281 ataaattatc gattatcaca gcagcacaaa cattgattga ttaacaaata cacgcgaact
    29341 agccaaaaac agcagctgct gttcttgact agttcatttt ttctttaaaa aaacttcatt
    29401 acgcgatata ctggcaaaaa gaaagaagag cgggagaaga tgaaaaatga tcgaactatc
    29461 aggattaaca atgacagaac aattcgcaga ggcaattggt cattccgcaa tgccaggtga
    29521 caatctgata ttgactggtg atctaggtgc gggtaaaaca acgttgacga aaggaattgc
    29581 ccaaggatta gggataacgc aaatgatcaa aagtccgaca tacacgatta ttagagaata
    29641 tagtcaaggt cggattccgt tgtatcatat ggatatttat cgggtagcag ctagcggagc
    29701 agatttaggg ttagatgaat attttgaagg tgatgggctt tccgttgttg aatggggaaa
    29761 tctattagaa gaagcactgc cggaggatta tttggaactg attttagaaa aaagtgatac
    29821 agatttagaa aagcggtatg tgaaactaat ctcatacggg gagcaaagtg aaaccttcaa
    29881 acagcgaatt ctagaaaaat gggctgaaaa tcatgagtga agaagttggg atcatcttgc
    29941 gggaagcagt gccaggagat gcaaaagaca ttcttttgat gatgggacaa gttaataagg
    30001 agacagaatt tttagttttg gatgaagcag aactcctgtt accaccagaa accttagaag
    30061 aagagttgga ttatatatac gaaagcaata ataatttatt gctgcttgca atctatgaag
    30121 ggacgattat tggaacagca tctgtcaagg cagacagtca atttcgtttg tctcacgtgg
    30181 gagaagtcgg tatcagtatt ctgcaggaat attggggaat gggactaggc actcttatgc
    30241 tagaagaaat catcagttgg gcgaaggaaa tgggtgtact ttttcgattg gagcttgacg
    30301 ttcaagttcg taatgaaaga gccgtacacc tttaccgaaa aatgggcttc caaatcgaag
    30361 cggtcatgcc tagaggggca agaacagatc taggtgaatt tttagatgta tataaaatga
    30421 gttatttgat tgaatagaag aaataggaaa agagtgagaa actgtcatcc ttttgagaag
    30481 gcagaaaaac acacgaaggg actgtgacaa aagtcgtgtc gcagtccatt ttttttgaat
    30541 aaacggtgtt caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggaa
    30601 ctattttgcc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttgg
    30661 taaacggtgt ccaaaagctt gcatctgcgg taataagtcc aaccaaatga aaaatatgag
    30721 aagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaacctct
    30781 ccaataaacg gtgttctcct gccagctcct cggtcaccag tcacaatatt taataaacat
    30841 gagaagctat tttttgaaat tgctccttgt gactcggagt gaaacggcag gctcacgacc
    30901 tctttgtgtg tttcataagt attcttttac tttatagtca caagtttttt caatggatct
    30961 gtcccgaatt ttttttcttg ataaagcagg atttccgcac atgcacgact atcttccaat
    31021 gcatcatggt gatgatctaa ttggatgttc aaattttcgc acacagtgtt caatcgatga
    31081 tttggaaatt caggaaacag gcgacggcta cttttcactg tacataaaga aagataatta
    31141 ggctgttcaa gatgataata atctaagcat cccgctaaga cctttgtatc aaaagcagca
    31201 ttatgtgcaa cgactaaacg atttttttga aaatatggtt ggatagtttg ccagacttct
    31261 gggaatttcg gcgcattgcg tacgtcttct tgatggattc cgtgaatttg gacatttcgc
    31321 cagaaaaaag gagtctcagg ctgtatcaat gtatagaatt catcaacgat ctggctattt
    31381 tttaccatga ctagcgcaag agaacaagca ctataaggct gatgattggc agtttcaaaa
    31441 tccatggcaa caaaattcat ttctgtaaaa cctccttggg aaattactgt aacatacttt
    31501 tgaaaaaagc aaataaattt aacaaaaaac atagaatttt ttatgtaata tttgttgaga
    31561 aaagctagtt tttgaactca ttccttgtga tttggagtta gatggggatc atgacttctc
    31621 ggtattaagt caaatttatg caaagttgtg aggaaccatt ttcaaaaaaa tttcttacga
    31681 ctcgaaagta ggcgactttt tcacaacttc tccactaaac ggtgttcaat cagctgaccc
    31741 tcggtattag ctcaaaaaat agcaaaatat gaggaactat tttgccattt gtttctctaa
    31801 tactcaggtc aggacgctga tttcacaacc tctccactaa acagtgttca atcagctgac
    31861 cctcggtatt agctcaaaaa atagcaaaat atgaggaact attttgccat ttgtttctct
    31921 aatactcagg tcaggacgct gatttcacaa cttctcaata aacggtgttc aaaagcttgc
    31981 atctgcggta ataagtccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
    32041 cttattactt gatgcagtgc agctttttca caacctctta ataaacggtg ttcaaaagct
    32101 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga ggaactattt ttgtttggct
    32161 gttcttatta cttgatgcag tgcagctttt tcacaacctc ttaataaacg gtgttcaaaa
    32221 gcttgtatct gcggtaataa gcccaaccaa acgaaaaata tgaggaacta tttttgtttg
    32281 gctgttctta ttacttgatg caggacagct ttttcacaat ctctcgataa acagtgttca
    32341 aaagccagtt tctcggtatt atgccaaatt tatgcaaaat gtggaaagcc attttcacaa
    32401 aatttttctt aatactcgaa actaaacgac tttttcacaa cctctttatt tcaaatctaa
    32461 ttctactgga caatgatcac tgcccataat gtccgtatgg atctttgcat cagctagtcc
    32521 tggctctaaa tctttgctcg tcaaaaagta atcgatccgc caaccagcat tgtttttcct
    32581 tgaattgaat cggtagttcc accaagagta gacaccttcc tgggttggat aaaaataacg
    32641 gaatgtatcg ataaacccgt tatccaacag tcgagaaaga gccgctcttt cttctggagt
    32701 gaatccagca tttttttgat tcgttttcca gtttttcaaa tcaatctttt gatgagcgac
    32761 atttagatcc ccacacacga tgactggttt ttgttttttt agattttcca aataattata
    32821 aaaggcttct tcccattcta atcggtaatc taaacgtttt aattcgcttt gagaatttgg
    32881 cgtgtaacag gttacaagaa agaaatcgga atattccaaa gtaatcagac ggccttctgt
    32941 atcatggata tcgataccca ttccgtaagt ggcattcaat gctggttttt tagcaaaaat
    33001 cgctgtgccg gaatagcctt ttcgttccgc gtaattccaa tattgataat aacctggaag
    33061 atctaaatca atttgtcctg cttggagttt cgtttcctgc agacagaaaa aatcggcgtc
    33121 taactcatga aaaacttcta aaaaattttt atttacgatg gcacgaagcc cgtttacgtt
    33181 ccaagagata aatttcaata aaagcacact ccttcgaatt aatttcctta cctttgcgat
    33241 actattgtac ctcattttag taaaaaatgg tgtaagatga aagtaactaa atgatccagg
    33301 aggaaagtag atgaagattg gtgtaatcgg tgtagggaat atcgcagaaa aagcatattt
    33361 accaacttat gcgaaaaaac aaggaacact tgatttttat tttgcaacaa gaaataaaga
    33421 aacaaaagaa agaattcaaa aagagtacgg gttccaacat ctatatgaaa cgatcgatga
    33481 gcttttagaa gaaaaaatcg aagcatgtat gatccacgca gcaacaaaag tacattatga
    33541 attagccaaa aaatgtctag aaaatggtat ccatgtttat attgacaaac cactgagtgt
    33601 ccatctaaat gaagttcgtg aattgcaaga attagctgat aagaaccaaa taattttgat
    33661 gatcggtttc aaccggcgct ttgctcctat ggtagaagaa ttgaaacgta tcccagataa
    33721 gcggttgatc cacctgcaaa aaaatcgaat agcggcaaaa gaaacgacag agttcgtcct
    33781 ttatgatttg tttttacatc ttattgatac agctgtgtat ttattagatg aacccatttt
    33841 gagaacaaaa taccagataa gagaaacaaa ggaatttttg gaatatgcca ttttacaatt
    33901 agaaacacaa aatcaaacgg ctattttaac gatggattta gcaagtggcg ctaatataga
    33961 aaaatatcaa gtaacaagta aaaatggtac ttatgaagta aacgatctga cccagcttgt
    34021 cattcaagat gctgatggaa aaaaagtgaa agaatttggc gactggacaa acacgctagt
    34081 caaacgcggg tttgagccaa tggtagaggc cttcttttct cagatccgtt taggtaaatc
    34141 ggaaacagaa ttgcttaaac aaaaagaagt tgtaagaagt catgaattat gtgaggaagt
    34201 gcttaaagat cactaccgtc atcaactttg aacaagactg gcaagattgt attaagcgtg
    34261 atagaataat gaggacgttt gagaaaagaa aggaaaatcc tcagtgaata aagaagaaat
    34321 agttgaaagc ttagccacga ttactatatt gtttgatgaa ccattgatga actatacgtt
    34381 tacgaaaaca ggtggaccag ctgatgtcct tgcattcccg aaaaaacaag aagaagtaaa
    34441 gcaaatcatc gattattgcc gaatccatga tataccttgg atggtattag gaaatgcaag
    34501 caatctgatc gttcaagatg gcgggattcg cggtgtagtc atcatgctga ctgaaatgaa
    34561 acagattcat gtcaaaggaa cgatggtcat tgcggaagca ggtgcctcac tgattgatac
    34621 gacttatgca gctttggcag aatcattgac tggatttgaa tttgcttgcg gcatcccggg
    34681 aagtgtcggt ggagcggttt atatgaatgc aggtgcgtat ggcggagaga tcaaagatgt
    34741 ttttgctgaa gtggatcttt tgcttgaaga tggaacattg aaaactttga caaaagacga
    34801 aatggtattt tcttatcgtc acagtaaagt ccaagaatta cgtgctatcg tattggaagc
    34861 tcgattcagt ttgcaaacag gggattatga agcaatcaaa gcaagaatgg atgagttgac
    34921 cgaattgcgt caatcaaaac agccattaga atacccttca tgcggaagtg tctttaagcg
    34981 cccagtagga cactatacag gtcagctgat ccaacaagca ggattgcaag gcctgaaatg
    35041 gggcggtgca caagtctctg aaaaacatgc tggattcatc gtcaatatcg atcatgcgac
    35101 agcgacagat tatattgaat tgattgcgca catccaagaa gtaatcaaag aaaaatttga
    35161 tgtgtcttta gaaacagaag tgcggatcat tggagaaaaa caagcaatat cagaaacaag
    35221 cagaagttta gccaattaat aagtaatgga acttaaaata agtaaaagag caggacggtg
    35281 gccgtcctgc tcttttttgt ggttgtttac gcaaatggcg caaatcttgt acattttaac
    35341 acaataaaac aaaaaatata gaatatttcc tcataaattt ttccattttt tgcaatattc
    35401 tcaaaaaaaa gataaaaaat gagaataact gatgaaaaaa caagcataat ccatcataaa
    35461 atcagtatag gtgaaaagat gaactttcat ctgcatatca agtaattggt atgaggcatt
    35521 ttttgaggtg taggatatga agttggatta ccttatagaa gaggaagata gagaaaaact
    35581 gctgctagct agatatttag aatttgagaa aaagaattat gtagtgctgc ctaagttata
    35641 tgaaacatta ggtttgtcta aatttaaaac aagaaattat atcatagaat taaataaaga
    35701 atttactgaa ttcaaaacaa agccagagat ccaaatttca gaagaagcag agatccatgt
    35761 cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga atctactttc aacgatcaaa
    35821 aattgctcga ctgttaacgg agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga
    35881 gaatcatttt ttaagcatat caagagccta tgtaaaaaga agagaattag tctcttttct
    35941 tgaacagcat caaataaagt taaagaaaaa catattagtt ggggaagaaa taaacatacg
    36001 gaacgtatta tttggggtat atttcggggt ctttaatggt ttttctatgc catttagaga
    36061 aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt gaatatctgt tcaatttaaa
    36121 actgtctgca acaaaacgaa tcaaattcaa tttgctaacc actattagtt tgttgagggc
    36181 ctatgggaat caaccaatcg gaaaacgttt cttttcagat gaatacttta agagctcaga
    36241 aggaatgaag atcatcaagg atttatcaca tatttctttt cttcatatcc ctaaagagca
    36301 gatcattgat gagatttctt atatcatgtt gtttttgatc acggaagaag aatttgaatt
    36361 agatggcgca tttggacttg cggaagctca ttttcatgat atccacatga ttagtcaaaa
    36421 aatgtcagtg gaattgattg atcaattacc attttcgatt gaaatctctg aagaagaaag
    36481 actatatata cgaaaagaac ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat
    36541 attcgacttt gagatgacta gctttacgtc taaaaagcag ctacagtttt tcttcgagac
    36601 gtatccaata ttcagtacag caatccaaaa ggtcatacaa gagaataaac gctatttctt
    36661 gggaaaagat gaacgtttca tgattcgctt attttatgat tatctctttt tattggtcgg
    36721 tatttgtccg gtatcatatt ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg
    36781 atctgcttat accgattaca ttatttctca agtgaaagga tttaaaaatt acaatattgt
    36841 gattgagagt cgaatcactt ctcgtacgaa tatttattta tctgattgtt tacttgaaaa
    36901 atttaataaa gaacagataa tttggaaaaa tccaccgact ccagatgact gggaatattt
    36961 tggaaatacc atcatcagag aaaaaaatag acatttaact gaaaaaatca gctagtattt
    37021 atggatggca gctttgtctc acatatttat tagctgattt atgttttttt caaagaaaag
    37081 gtaactagat ataactagat ataactagat atattactta acaaaattaa aaagtacata
    37141 agttattatt ccgattttct tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt
    37201 ccatcttctt ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg
    37261 tttagacaag agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact
    37321 aaataaacac gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa
    37381 gtttatgatg cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt
    37441 tcaggcaacg acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc
    37501 ccctatcact attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt
    37561 gacttcctat aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa
    37621 caaaacatta tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat
    37681 gactgatcct acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt
    37741 gacagatata aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc
    37801 acagtttgaa agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct
    37861 ggtagaaagg ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa
    37921 caatgggaat atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt
    37981 tgatccaatt gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca
    38041 aacagctatc aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga
    38101 tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat
    38161 gaacgggaca gtagatacta caactccggg agaatatccc gttgtattaa cttatataga
    38221 ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc
    38281 ttcatcgact gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga
    38341 aacacatatg agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga
    38401 tcgagtgcca aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc
    38461 tgattggatg caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt
    38521 acttcactca gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta
    38581 tgtttatata ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga
    38641 aaaaataaac aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc
    38701 gttaaaggat gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa
    38761 acaaacaggt gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact
    38821 ccatgctcag tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca
    38881 aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc
    38941 aacagggaaa gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc
    39001 agaatctaat aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga
    39061 aaatgaaatt ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca
    39121 gtcggaagat cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac
    39181 tattgattcc accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc
    39241 cgcacctggt ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg
    39301 aacatgggta tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc
    39361 tctatctgtt atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga
    39421 ggagggatag aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac
    39481 cacttcaaca aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc
    39541 aaaaagaaga tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga
    39601 cagatgaagc acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa
    39661 gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc
    39721 gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaaccagatt
    39781 accaaggaaa agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc
    39841 tccatacgga cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc
    39901 tttataaaaa aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag
    39961 attatgaaaa agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa
    40021 gtcctcagta ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt
    40081 aatactatcg gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa
    40141 gtattgtcac tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga
    40201 ataaaattct tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat
    40261 acactgaaaa caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt
    40321 cccacgtcta agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga
    40381 ctttcaatga ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt
    40441 aactccacac tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat
    40501 ttatctgctg aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag
    40561 gaaacaactg ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa
    40621 taaacaaggg aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta
    40681 tcccacattg aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa
    40741 ataaaagata acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc
    40801 agaaaataag ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag
    40861 tgttggttcc agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag
    40921 attccgtttg tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga
    40981 ttcataaaat aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa
    41041 agctatatga cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta
    41101 taaggagaaa agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc
    41161 acttcttgct atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag
    41221 tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa
    41281 agaaatgaaa gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc
    41341 agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc
    41401 cggaggaaac acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa
    41461 agaagaagga atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg
    41521 aggaacgaat gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga
    41581 ttggctagat caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga
    41641 aaagaggaca catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt
    41701 attgaataac gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat
    41761 taagaaaaat ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa
    41821 agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt
    41881 tattgaaaaa tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac
    41941 tggagatggt atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat
    42001 acaaattcat ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg
    42061 agaaggagca atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag
    42121 ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga
    42181 ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac
    42241 aactggaaag acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc
    42301 aaaaacagtt gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg
    42361 tgccacagcc atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc
    42421 aggaatccat tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa
    42481 agaaaaccag ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg
    42541 aaacaaccgg atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg
    42601 catacaagca gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg
    42661 ttgtgaaatc agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga
    42721 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt
    42781 cttatgtttt taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
    42841 ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    42901 ataaacggcg gaagcagaag aaattccttc ggaaataagc tgaaattcac aaaaatttga
    42961 aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc
    43021 ttttttgatc agcatgattt tattcagtta tcttcaaaat gatttttccg atgtttttgt
    43081 ttgctttcat atattcatgc gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga
    43141 ttggccgaat cttgccattt tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga
    43201 gctctgcttt atactgatca cttctaggtg tcaataatgt accaagcagt tggacacgtt
    43261 tcccaatcaa tacaccaagg tctatctcag gcacgatggt cccacctagc ataccaatca
    43321 agacccaacg tccatcaacg gctatagaag ctaaattctt ttcccagtaa gaagcgccga
    43381 tgaaatccaa aataacatcg actccccgac cttcagtgat ttttcgtacc tcttcttgaa
    43441 agtcttgttt tttatagttg atcaattcat ctgcaccaag ttctttacaa aaggcgagtt
    43501 tttcttctga tcctgcagta acgatgattt tcgcgtgaga aagctgtcta gctaattgga
    43561 tcgctgctgt tcccacacca cttgctcctg catggatcaa gacagtttcg ccttcttgta
    43621 attttccaag ccagtaaagt gtttgataag cagtcaaaaa aacttctgag attccagcag
    43681 cagaaacata gtctagcgta tcagggagaa ggattgcacg atcggcaggc atcacagcat
    43741 attcggcata accacctaga ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg
    43801 agatatcact tttattctca actacctctc cagctacttc gactcccaaa atagggtagg
    43861 gaggtttcaa agagactgat tctcttttca taatatctgt tcgattgaca gctgctgtat
    43921 ggactttgat cagtaattcg ccttgttttg gtttaggaac aggcacttca atttgttcta
    43981 atacatctac agaaccagga tgcttgattg aaatggcacg catggatcta ttcctccttt
    44041 gcccattctt ggaatgctgt attcaactct ttgctacctt cagaaagtac tagagtcgta
    44101 cgagaaatat ccgtttcttc cactagagcg gcaagtacag cagcaactgt agagcgagtg
    44161 atcgtttttg atgccttctc attaggctta gttaattgaa tggaagtaac ttcttcatcg
    44221 ttagtcaatg tgactggttg aagaatgaca aaatctaagt tagttcgttt cagccattca
    44281 tctgcataat gtttagtaat ataataatct gtcatggact caggccattt tgctggttca
    44341 tctgcataaa cagcactgac catcagatag cgagagacat gcgcattttc agctgcgatc
    44401 actgttttga ttgcgccatc caagtctact tgcagaagat tctttccttg agagccggca
    44461 gcaaaaatca atacatcgat gcctttgaat gcttcggtca ttttttccac ggaccaagtt
    44521 aaatcaaaag agacatacga aacattctct ttttttacta atgtttggga agcaacatct
    44581 ctcacccctg caaaaatttc atgggaactt ggagctaaat cttttatcag gtgtcggcca
    44641 atttgtccgt ttgcacctac aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat
    44701 ctttccattc aatagtaaca ccaaaagaga aaaaaatcag agatcggcta cgatgactat
    44761 cagttagtaa gagaagggtg cttgaagtat cttaacaaga cattgcatac tgctgcagtc
    44821 gcaagacctg cgacgattcc ttcaggtata ccacttgtaa aaataaccgc tagaatagct
    44881 gaataaacag catttaaatt tgttccgatg actttggcat aatcttgctg gaataaaaag
    44941 tagatcatat tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc agctaagaat
    45001 aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca gacctttata aacgaaataa
    45061 ggaaccaccc caatcaagat gcgaggaata aacgcgatca gcaaggcttt ccagcttcca
    45121 tgttctgttc cgatgactgg gataaacgga gaaaagacaa aagacaaagg tgtttggata
    45181 actgtgctac ggatcatgct gatcagtcca aatgtaccgc caagaaaagc acctagtctt
    45241 ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca tggttgttgc attgattgga
    45301 ccgataggga taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc
    45361 gcagttaatg taaagttttt tgtatttttc ataagtactc cttttttcta agcatttctt
    45421 tcattaagag tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt
    45481 ccaaaatctc cacaagctaa aagagattct cttggttcaa tttcctgata gccgacagat
    45541 ctcaatgttt ccagattacg ctgagtgatc gggttttgat acatattcgt gttcatggca
    45601 ggggcaatca attttggtgt tcccactgga agcgccattg cgactgttga aagaagatcg
    45661 tctgcaatgc cgtttgctag cttgccgata atgtttgctg tagcaggcgc gatgagaaac
    45721 aagtcagctt gttttgctag ttcgatatga ttgattacct gcggatttga ttcttgcatg
    45781 acgtctgtat ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt gataaaatgt
    45841 gtgctgcttt tagacataat cacatctacc tgggcaccta atttgacgaa ttgacttgta
    45901 agatcagcgc ttttgtaagc agaaatactt ccagtaacgc ctagtaagat ctttttcatt
    45961 ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc aatctcagat ttactttgag
    46021 tttcttccac tgttccatct ttagataaga gatagccgtg gtgtgtctcg ccatggattt
    46081 ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt tatcagacta ttttttgcga
    46141 ctgccagaag agattccttt ggtacatcta ctaatagttt gaaaccaatg atttttgctt
    46201 ttggctgcca ttcacgtaga taagaaatga tttttggtgt tttcttcaaa acgagcacaa
    46261 gatgctcggt gttagaagag atctttgaaa cagtggtatc tgtctgttca ttttccgcaa
    46321 accaattgtc cagttcttct tgcgttgaag aagtgggtag gttttttgcc agctgttctt
    46381 ctgaaaaact aaaagcagga gtaaaatcac tgactgccat actgtggata atcgcatcgt
    46441 agtgttcttt tttcgttagt tcttctaatt gtaaaaataa gtccagtgca gattcgatcg
    46501 tataaatcgt tatattggag tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat
    46561 cgattgtggt atccggattg gcagctaaat aatcagctaa tgcttgccct aatcgtcctg
    46621 tcgaatgatt tgtaatagca cgtacttgat cgattttttc agtcgttcct ccagctgtga
    46681 ttagaatctt catttatggt cacctacatt tactagtgtt cttccttggt gtgttccagc
    46741 aagtagttta gatgctgttt caggcaattg atccagagta atctcttgga cgaccagttc
    46801 atctgcaatc tgcaaatccg ccatctgttg ccataaagaa agacgttgat ctattggtac
    46861 attgacagaa tcaattccaa tcaactggat gtttcgcaga ataaaaggca agacagtcgc
    46921 gtttagtttg attcccccag cattgccaca taaagctact gcaccgttat aagagatcaa
    46981 tggaagaaga ctgcttagct gctcgccacc tactgtgtcg atgacatagt cgatttgctg
    47041 ttttcctaaa gcttttgttg tctctggcag aaattcatca ggtcgtacga cttgcaaagc
    47101 acctagactt tttagccatg tgacagactc ttcttttctg gaaaaggcaa tgattgactg
    47161 gaatcctagt ttatgtaaca gggctatcgc tgtactgcct acaccgccag aagcaccagt
    47221 aacgacaact tttttatcgg aggtcatacc ttgacggatt aaagcgttca cacacaacat
    47281 agcagtaaac cctgctgtac caaggatcat cgcttgacgc agcgtcatat tttttggtag
    47341 aggaactaac cagtctcctg gtactttttg atattggcta taaccgcctg gatgggtgac
    47401 gccaagccca aaaccagtag caagtacaag atcttctttt gaaaaacgtg gattgttact
    47461 ttggacgaca gttccggata aatcaatccc tggagtcatc ggatatgttc gtatcacctg
    47521 accagactcg ctcattgcca atgcatcttt ataattgaca tcagaatagg caatttttac
    47581 caagacttca ttttcagcca actcagtaaa tggcagatca ctgattgctg tatggaaaac
    47641 aggttccttt attacctgaa aagatcgaaa agcgttcatt cgtgagacac tccttttaaa
    47701 attttctttt atcataccat aaagcctact cttttattct tgttcttccg taaatttaga
    47761 caaaaattag gtgaaaaata aagaatcaaa caactagaat ttattccaaa agataaagaa
    47821 atattcagca aaaaaaattt tacaaagggt tgcctttttc aataaatttc gtatactaat
    47881 acgagtggta gtaaacagga agtttactat tagaaaactt cgattcggag gaaattatgg
    47941 aaattggtga gaagttaaga aatttgcgca tccaaaagaa tttgacacaa gaagaactag
    48001 gcgaacgcac agatttgtca aaagggtata tttcccagtt ggaacgagat ttaagttcac
    48061 catcaatgga aacattcttt tctattcttg aagtcttagg tgtgactcca gaagaatttt
    48121 ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa agaagatcat acggtttatt
    48181 tcgatgaaga aaacgggtat gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa
    48241 tggagcctgt cttgctgact tttgaaggtg caggagaata taagacgttt gaaccttcat
    48301 tatctgaaac gtttattttt gtattagaag gggaattaga gttaagctta ggagagacgg
    48361 tctatgtggc aaaagcagga cagtcgatct attatcaagc gacaaaacaa catcaattaa
    48421 ggaatcatct caaaaaacga acaaaagtat tgattgttgc aacgcaatca tatttgtaat
    48481 atagggggta aaaacgtggg gaaaccaatc atttcatttg atcaagtagt gaaacgctat
    48541 gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg aacaaggaaa attttataca
    48601 ctgcttggtc cttccggctg tggtaaaaca acgatcctac gtattatagc tggattcacc
    48661 gaagctacag aaggggacat ctattttgaa gggaaacggt tgaatgattt gccagctaat
    48721 aagcgacaag taaatacagt cttccaagat tatgcacttt ttcctcatat gaatgttttt
    48781 gataacgttg cttttggatt gaagatcaag aaaatgtcca aatcagatat cgaaaagaag
    48841 gtaaaagatg ctttgcgtat ggtccagctt cctggatacg aaaaacggga gattagtgaa
    48901 atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa
    48961 gtattgctat tagatgaacc gttatcggcg cttgatttga aactacgtac agatatgcag
    49021 tatgaactgc gggatctgca acaacgatta ggaatcacgt ttatttttgt tactcatgac
    49081 caagaagaag cgttggcaat gagtgatgag atttttgtca tgaataaagg gaaaatcgtc
    49141 caaagtggta cacccgtaga tatttatgat gagccaatca atcatttcgt tgctgatttt
    49201 gttggggaga gcaatatcgt ggacggtgta atgatcgaag ataatctagt tgagtttgtt
    49261 ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt
    49321 ttacgcccag aggatttgac gatcacgaca ccagataaag gaaaactagt cgtaacggtc
    49381 gatactcagc tgtttcgtgg tgtccattat gaaatcatct gttatgatga acagcaaaat
    49441 gaatggatgg tccattcaac gaaaaaagct aaagaaggaa gcaaagtagg attggccttt
    49501 gagccagaag atatccatgt catgcgtttc aacgagtctg aagaagaatt cgatgcacgt
    49561 cttgacagct acgaagaata ggggggacaa gaatgaaaac aatgcgcagg atctattccg
    49621 tcccttatat tttgtggctc ggtttattcg tgatcgcgcc tgtattaatg atcatttatc
    49681 aatctttttt tgatatgaat gggcaattca cgctgaataa ttatctatct tattttacgt
    49741 cggggacatc gctttctatg acgatcaatt ctgtatggta tgcatttttg atcacgtttt
    49801 tcacgctgat catcagttac ccgactgctt attttttaac aaaactgaag cacaagcaat
    49861 tatggctgat gctgattatt ttgcctacat gggtgaatct gctgcttaaa gcttacgcat
    49921 ttattgggat tttcagtgtg cacggcagcg tgaatcattt ctttgatttt atcgggatcg
    49981 ggccgaaaca gatattattc acagacttca gttttctgtt cgtggcaacg tatatcgaga
    50041 ttccatttat gattatgcca attttcaatg cattggaaga gatgaatcct tctttgatca
    50101 gtgctagtag agatttaggc gcgaatagct ttgagacctt ccgacgagtg atttttccgt
    50161 tatcattaaa tggtgtgaaa agtggtgtcc aagcagtatt tattccttct ctttctttat
    50221 tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac tttaggaacg gctatcgaac
    50281 agcatttctt ggttacacaa aactggggga tgggttctac aatcggtgtg attttaatca
    50341 ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa
    50401 tgaaaaaatt taaatggtcg tatctttatt tagggatcgt ctttttattc ttatatgcac
    50461 cgatcttcta tctgattttt tattccttca atgaaggagg aacgatgaac agctttacag
    50521 gatttacttg ggaaaattat gaagctgttt ttgaagatac acgtttgatc attatcgtat
    50581 tgaatacgtt catgctggct ttcttatctg cattgatcgc cacgatcatc gggacatttg
    50641 gggctatggg aatctattac acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga
    50701 ataacatctt attagtctct cctgatgtta ttatcggtgc tagtttcttg attttcttta
    50761 cactactagg attcggatta ggttttcaga gtgtattgct ttcccatatt gctttcagca
    50821 ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga aatgaacgat tcaatggtcg
    50881 atgcggctag agaccttggt gctaataatt tccaagtagt caaaaatatc attttgcctt
    50941 atctttctcc aggaatcata gctggatatt ttatggcatt tacctattct cttgatgatt
    51001 ttgcggttac atttttcgtc acaggaaatg gctttacgac gctctcagta gagatctatt
    51061 ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt
    51121 tctccatgat attggtcatt ggatactact ttattagtaa agaaaaccgt ccaaaacggc
    51181 tgaagaaaaa cagaagagta cgcgaggggg tggcacggct gcgatgaaaa aattacaatc
    51241 tctgttttta ggtattctca tcattattgc ggtgctgctg cttggtgttc gtcaacttga
    51301 aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc tataactggg gagattatat
    51361 cgatcctagt ttgatcacga aatttgaaaa agaatatgga tacaaagtga attatgaaac
    51421 atttgattct aatgaagcga tgttcacaaa aatccagcaa ggtgggacaa actacgacat
    51481 cgctatacca agtgaatata tgatccaaaa aatgatcaaa gaaaaactag tacttccatt
    51541 agatcattcg aagatcaaag gattaaataa tattgatcca cgatttttag acttggattt
    51601 tgatccagat aatcagtatt cgattccata tttttgggga actttaggga tcgtatataa
    51661 cgataaattc tttgatgcaa atcaaattaa gcattgggat gatttgtgga agccggaatt
    51721 aaaagacagt ttgatgctga ttgacggtgc acgagaagtt atggggcttt ctttgaatag
    51781 tttaggctat tctttgaata gtaagaatat ggcacagctg acagaagcag caaataaatt
    51841 gaatcattta acaccaaatg tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat
    51901 caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa gctgccgata tgatgtggga
    51961 aaatgaacat ttacattatg tgatcccacc agaaggatcc aatctttggt ttgataatat
    52021 cgttatgcca aaaaccgtga aaaataaaga aggtgcctat gatttcatca actttatgct
    52081 agaaccggaa aatgcggcac aaaatgcaga gtatattggt tattctacac caaatgaaga
    52141 agcaaaagcg ttattgccaa aagacatttc gtcggacgaa caattctatc catctgatga
    52201 cacgatctcc catcttgaag tctacaaaga tctaggatcg aaatacctag gcatctataa
    52261 tgacctattc ttagaattca agatgtatag gagataatgg caaaactcag taagaatggg
    52321 gacttatcaa cgttctaaag agctaaaaat cgtccttttt ctatctgagt tttccaatct
    52381 tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat tttatttttt ctaccacgtt
    52441 tttctaccag agaaaagtgg acaaaaccat tgtaaaatag ggcccgagac gggctagtaa
    52501 atctaagatt gaaaaagtta cagaaggata aagtaattgg aaactatgca aatatactta
    52561 acccttctgt aatctattaa taaaactaat atatgaatcc agttcaatat ttgtagctat
    52621 gaaaaaacat aaaaatcata ttagataaaa actaaattgg tagataaatt attttgtcag
    52681 atatctgagg caaatattcg actatttgtg tttcaaagta aaaattggaa tacaaatata
    52741 gaatagacat attgcagccg atagtttatt atgcttttag acacattaaa ctggaggaaa
    52801 aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct gatacgacta acagggaata
    52861 tgtcttctgt cactgccgag aaaatttcga agaatctagg tatctctcgt cagaatgcca
    52921 gccattattt gacgagatta gtggaagata aaaaggtcga gaaattaccg ggaaagcctg
    52981 ttttgtggaa gcctttagat gaatatgcgg ttgtagataa tacggagcaa ataaacgaag
    53041 cctttcattc tgtagttggt catgacggaa gtctgagaga ggtcattcaa aaatgtatag
    53101 ccgctgtcaa atatccgcct aacggactca gtgtattaat aaatggagcc acgggtgttg
    53161 gaaaaagttt tttagccaca aaaatttttg aatacgctat ccatgagcaa attattgaaa
    53221 aagatgcacc atttgctatc ttaaactgtg ccgattatgc ggacaatccc gaactgcttt
    53281 cggcaacact gtttggatac aagaaggggg cctttacggg ggctgaaaag gataccgatg
    53341 gtttattggc aacggccaac aacggctatc tttttctcga tgaggtccat cgcctatcga
    53401 aagaaaatca agaaaagctc tttctattta ttgatactgg aaactatcgt ccagtcggtg
    53461 aaaatgtaaa ttggcattct gctaaggttc gttttatttt cgccacgacg gaaaaaggtg
    53521 aaaattattt attagacacc tttgatcggc gtattcaaat tagtgttatg ttgccaactt
    53581 ttgaagatcg ccccattcga gaacgattag agctgattca attatttttt caaaatgaag
    53641 cggatgtttt acaaaaagat attattgttt caagagaagc cttgtcttta atgttatctc
    53701 atccctttag tgggaatatt ggtaagctga agaatatcat taaaatcagt tgtgcggatg
    53761 tgtatagtcg aacacaagaa gaaccattaa cgattgggaa aaatgagatc ttgattcagt
    53821 taaatatgct ggaatccgaa gtagaaagct tgccgattgc tagtttattg ataaagaaaa
    53881 atcaaaagat aaaaacggat cgaatcaaac cgtttgatta tgattcgatt ttggaaaaaa
    53941 ttactcagct gataaatgaa ggtaatttca atgaaaattt ttcttcgata aagaatgaag
    54001 tacagcattt ggtgcatcac attgataagg attctttttt atcaatgagt agaaaattga
    54061 tgattcagat gtttagaaag gtctgggtat ccgttttagg aaaaacgtac ggattgacga
    54121 cgagtggacc tgttgcagaa attattagca gatcctatgt catttcccca agctataaga
    54181 cagacttaac cgggataaat cgtacattag ctgcaaatct acctcggacg acttacttat
    54241 gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa ggacgagaac tttctaagac
    54301 tgcttattgt cgtgacttta tctgattatg tagatgaatc catcgagctg aagggcctgt
    54361 tgcttgctca cggagatagt acggctagca gcattcaagc agtagtgaat caattatgcg
    54421 gcaattatgt ttttgaagcg ttggatatgc cagtagagag taatgtgtca gagattgtcg
    54481 aatcgacaag gaatttcgtg aaacggcaag tgaatacaga tagtctagtg ctgatcgtag
    54541 atatgggctc tttaaatcaa atttacacac agatcaagaa cgatttaaaa ggggagttac
    54601 ttcttctgaa taatttgacg acttccattg cacttgatat tggcttgaag atgacgatga
    54661 atgcgccatt taaacagatt gcggaggatg cagcagacaa gtattcaatc aatgttcaat
    54721 actttgaagg cttttctcaa agtagcaaca ttatcatttc ctgtatgtcg ggtttgggga
    54781 tttcggacaa attgaaagat gtttttcgtc atcatttatc ggatgaatcc atcgaagttt
    54841 tcacgaagga ctacagagaa ctacgtgaat taatcgacca aaatgatgaa acttattttg
    54901 acaagacaaa attggtcatc acgacttcgg atcttccacg ttccttttca attcctaata
    54961 ttaatgttta tgacatctta gacggtgatg gagcagccaa tttggagcat attttgagtc
    55021 ctgaaatgaa gccggcagaa tttaaccgct tgattcagga gctagtaaaa ttcttttcgt
    55081 tggaaggaat tgcggaccgt ttgaactttc tgaacccaac gatcgtgatg agtgaagtgg
    55141 aaacagtcat ttctaaatat gaaaattact atcatttcac tttaaatggc cgtgtaaaac
    55201 taaatctcta tatgcatacg gcattgatga tggaacgttt attcttggct cggacagaaa
    55261 aatctgaaga accgaattat gatttgaaac cagatgaaga tgaattttat tatgtttcta
    55321 gaagtatttt tcaatcaatg gagatgaaat ataattttaa agtcaatgct tatgagcttt
    55381 cgctgctcta tgaattatta ggaccgtata ttcaaaaata aagtgtctaa aaaagtgtct
    55441 aatctgtttt ttttaggtta gacacttttt ttaatgtgaa ttaacgtgat taaaccaatg
    55501 tttttaaaag ttggcacagg aattgcttat ataaaagtga acagaaagaa aggaattaac
    55561 catatgaaga aaattctaat agcgactcac ggtcacttag cttcaggctt tcttagcagt
    55621 attcaattac tgacggggaa ggttcaagag attacggtga ttaatgctta tgtggatgag
    55681 tgtgattttg aacaggagtt gaatgtattt gtaaaccagg tgaacgagca ggatcagatt
    55741 tttgttttta ccgatttatt tggcggaagt gtcaatcaga agattaccaa aacctttttg
    55801 gagaaagaga ttgctgtaac tattttgtct ggctttaatc tgccaattct gctcgaaata
    55861 gtattggcaa ccaaggaatt aacaaaggag gatattcagg aactggtaaa taaatgccaa
    55921 caggaattaa agatgaccat tcttgatgag gtacatgtag taaatgaaga ggaagatttt
    55981 ttttaggagg aagaaaaatg attaaagcag tgcgaattga tgaacgatta attcatggac
    56041 aagtagcgat gacttggaca aagagtctaa atttaaccgg gttagtagta gcaagcgatg
    56101 aggcggctac caacgatatt caaaagatga cattgaagat ggctgcacca tcgaatgtca
    56161 aagtcattat taaaacagtt gatgacgcga ttcatttgtt aaaagatccg cgggcgaagg
    56221 acatgcgctt gatggtttta gtccctaatg tcaaagatgc ggtaaaagtt gcgaaagctt
    56281 tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg gaaaatgaca ccgccatcgg
    56341 aagataagaa aaccttatca aaggaagtga tgttaacgac gtcagaaatt caagacttaa
    56401 aagaattagt tgaactttat ccagataccg tttttcagcc tacaccggcg atggaaaaac
    56461 gttcagctat ctctattttg agtgattttt agaaaaagga aggagaaaaa ttatgtatga
    56521 tgcatttgta gtcgctttgg ccgtttttat tggagttgct ggacatgaaa tgttcgggat
    56581 gtccatgctg agtcgtccga ttgttgttgc tccgttagca ggagcgttga tgggggattt
    56641 gcagacgggg ttgattgtcg gggcgtcact agaagccatc tttatgggtg tcgtcaatat
    56701 cggaatctct agtacggcgg agccttcttt ggctgctggg ctggctacag cgttcgcaat
    56761 caaaatggga ggaaacgtgg atgcgattat tcccattgtc tttccactag cagtattagg
    56821 attacagtta atgaatatga ttttttcttt tgtatgtgga ccgatggctg ctaaattttt
    56881 caaatttgca aaatctggtg atcaaaaagg aatcatcaat ctgcacttct ttatgtggtt
    56941 tgtccactat gcgctttatg ctcttattcc attctttgcc gttttatttg gagcagatgt
    57001 tgtcaaaagc gtcttggata atattccaga ggtcatcatg aacggattaa cagtcgctgg
    57061 aaatctgctg ccggcagttg ggatggcgat gctgttacgg atgctttgga gtaaaaatat
    57121 tgctgtgtgg tttttctttg gggccgttgt tatggcgtat ctcgagttac cattgattgc
    57181 aattgccgca gttggaacga ttattgcaat tgttatagcc caaagagatc tacaactaaa
    57241 aaatatatct gttcatgcag ttcaaagcga tgctgctgta aatgcaacgc ttgaccaaga
    57301 agaggaggat ttctttcaat gaaattagct gatggaattt ctaaagatga gaaaaaattg
    57361 gttcggaaga tgttttggcg ctcgatgact atgtatgctt cggtgaatcc ggtgacgatg
    57421 ggcggcggtg gtttcgccta ttctatgatg cccttcatta atcatttcta caaaagtgaa
    57481 gaagatcgag ctgcggcgct ggaacgtcat acacagtatt tttcgacgac cattccttgc
    57541 gcgtcctttg tcatggggat tgcggcatcg atggagaagg aaaattctga aaaagctggt
    57601 tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga tgggaccatt ggctggaatt
    57661 ggcgattcat tgttctgggg agtttggcgt gtcgttaccg cgggactagc gatcaactta
    57721 gccaatgccg gaaatatttt ggcaccaatt atctttttag ttatgttcaa tatacctaat
    57781 tacttgatgc gatactatgg cggcgtttta ggctattcat taggctctaa atatattgaa
    57841 aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag ctgctagtat cttgggtctg
    57901 atcatggtag gtgcaatgac gatcagtatg gtcaaattca ataccgtttt gagcttttca
    57961 atgaagggcg aagaactaat gaatgtccaa gaaatcctcg atagtatttt taaaggaatt
    58021 gtaccgttat tggtaacatt gagctgtttc aaattactag ataaaaaagt cggcgtcatc
    58081 aaaattattt ttggaatggt cgtcttggga attttattgt cattacttgg aatcgcatag
    58141 taggaagctg cagctactag gctgcagttt ttttagcaag gagggaaaag atgactatta
    58201 atgaagtaat ttattcaccc aaacacccat tttttaatgc tgagttaaac gacggaaatg
    58261 tgactacttc aaacgaatac agctactatc ggaatattcc tgatgtaaag ggattgacct
    58321 tcgatgtaag taatcaaaag acattagcaa accttgatgt ttgcgggacg attcaaacgt
    58381 tgacctttta tcgagaaaat ttactaactg aggaaaaacc aggtgtttgg gtcaataaac
    58441 agctctctca aaccaattcg ttatccttaa aggttgaagt aaacgggatt gtctaccatt
    58501 tgtcagaatc ggatcatcgt attgaagtcg atgtgattca agactgtttg cctagatata
    58561 ttcatcatta ttcgacgttt aaggtcattg tagtatcctt tgctcccata ctggctaata
    58621 agcgtttaag tatgcttgtt caacaggttt acattgtgaa tgaaacacac gaaagccttc
    58681 aagtttcagt gaaagatgtg ccttcgtatc aagagaagta ttcggatcag caaaatgtgt
    58741 tgatttctca aaaaggaaat aaggacacga tcgcacaaca ggaagtggca agcttcgcca
    58801 ttgcttttgt ggacccaaat gcttttgaag aagtaatgac ttttcaggaa tctgatatcg
    58861 aaaaatggct gaaggaaaca ttgaactatt ttgctcagct atttgggcag ctgtcaatgc
    58921 caaacgatag agttgtccat ctctatcgtc gtgcacttta tcaaagcttt tctagtttcg
    58981 gtatgaatcg acagggacag gtcgtcggca gcaattgggg cagctatcct gctactaatc
    59041 gaatttggaa taaggatatg tattacgcat cattgccatt caccatgttt gaaccggagt
    59101 tatgtcaaaa aacggttcta tggtttgatc aatatggcgt taagtttcct ggttcaaaat
    59161 ttacaggagg tatcaatcat tctctgagta attcgttatc cagtgcgtta ctggctagtc
    59221 tttattttga acatactggg gatacaaaat tctttgagcg gtgtccgcag gttctactga
    59281 atgcttcaag aattattgag gacatattag ctcaacgtcg gtcaggggaa ccaatgcttt
    59341 ttccatctgt ttggttatca gacgcctttg cacttggaaa gtatcatacc ggcagcaatc
    59401 tttgtctttg gaaggcctgt attggacttg cagaaatttt tgaagcccta gaacaactga
    59461 gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa ggaatcaatt tgtaaacaaa
    59521 tgactatcga tggtcctttt ggtgagcaat ttttggaggg aatcggtgat gaggagaaaa
    59581 caacctattc agtgaagcac tatcaaaaac cgattcttga gcaagggtta atctttttaa
    59641 gtgatgtgat tgtggatgga aaaattaatt tattgatgca tgatggagaa gaatcagaca
    59701 ctacgttgat gcctttttat caatttttag ataataaaga tcatttatat cagaacacca
    59761 tggccttttc tgctagttct ttcaatccaa cctatagcga ggaaatcaaa ggcatcactt
    59821 ggggattaga atccggcgcc acttttcctg gctttatcac tgttttaatg agcgagctgc
    59881 acaaccctca agctttcgta acccgattag aagaactaat ttgtttggca gatttagacg
    59941 gttcgtggtg gtggtggccg tatcgcttag aggctcaaca aggagaagtc gtgcgagact
    60001 ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt cagcctaatg atcagtcaat
    60061 atttcggatt gaaattcaaa aaaggagtat tgcaaattga tcctgttgat aatttaacgt
    60121 tttcttggaa gaatttgaag ttcggtcaag ccttcatttc gattgaatgt acgcaatccg
    60181 agttgagtat ttcaaatttg tcagaaaagc cattgaaaat aagtgatacg aaaaaaattc
    60241 ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag aaaaagatga gtatactaaa
    60301 aaatattacc caatttaaaa aaatccagag tcgagcaatc actcccgaaa atccaacagg
    60361 cgagaaaggt aaagctgcga tgttgccaag cgagctagga ccaagccgca aaggtcgtgg
    60421 cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg gatattactg gaacgggaat
    60481 aattcgtcat atgtggatga cgattcgtga tcgaacagaa tttggcagtt ttgttttacg
    60541 tgatttaatc ttaagaattt attgggatgg gtcggatagc cctgctgttg aaagtccttt
    60601 aggggatttc ttttgtaatg ggtttggaga gcgttgcgag gtgaactcgt tgccgatagt
    60661 ggttaatcct acagggggct ttaattccta tttcgaaatg cctttccaaa catccgctaa
    60721 aattacgatc gagaatttac atccgaagga tatgtcctca tttttttata cgataaatta
    60781 cgctttggtg gacgaattgg aagaaaatag cctatatttt cacgcaaatt ggcatcggga
    60841 acgcattacc aaattacagg aggattataa gttaattgat cgattaaagg gtcaaggcta
    60901 ttacgtgggt acttaccttg cgcttacctg cctagaacgt tattggtggg gcgaaggcga
    60961 gtttaaattc tacattgatg gggatagtga gtatccgacc gtaacctcaa caggttcaga
    61021 agattatttt ggcggggcgt gggcctttca tgagtacgat gagttcggac gtccacatgc
    61081 gaaaaactat tcaacagcct ttttagggta tccttaccaa tcaaatagag atgccacaag
    61141 agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc tttggtgacg acgcaatgcc
    61201 acgacatggt ctttatcgtt ggcatcttca agaccccatc tgttttaccg aagatttatc
    61261 ggtcacttta caacaaatcg gcaacgatga tgttcgctta tttgagcggc aagacgatgt
    61321 tgctagtgtc gcttactggt attcaacaga agcttcagga aatactaaac gtcatttcac
    61381 tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac atattcgaag ggaggaacct
    61441 atgatgatat atggggcaat tgaagctgga gggactaaat ttgtctgtgc agtcggtact
    61501 gaggaattaa cgattttgga aagagtcagt tttcccacaa caactccagt tgaaacaatg
    61561 aagcaggtca ttgatttttt taaacctttt aaggaacaat tggcaggcat cggggtcggc
    61621 tgctttggtc caattgatat tcaatgcaaa tcaacgagtt tcggtcatat cacatcaaca
    61681 ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc taaagcaata tttcaatatt
    61741 ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg gtgaatatgt tgctggttct
    61801 ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa caggtatcgg aggtggtgcc
    61861 ttgttcaatg ggactttcgt ggaagggttc agtcatccgg aaatggggca tatgttgatc
    61921 aagcgacatc caaaggacaa tttttcagga aactgtccct tccaccaaga ttgtttagag
    61981 gggatggcag caggaccagc gattgaaaaa cggttgggtg taaaaggcca gaatttattg
    62041 gctgacgatt cgttttggca gattgaagcc ttttaccttg cccaatgtgc atacaataca
    62101 acactgatgt tttcgccaga tagaattatt tttggcggtg gtgtaatgaa acaagaacac
    62161 atgaagaaaa aagttcagga taaatttgtt gagcttataa atggatatgt tgaaatacca
    62221 ccaattgata gctacattat tacgcccgaa ttaggtgata atgccggaat aatcggcgga
    62281 ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat aattaatgaa tgtttcattg
    62341 atactcatgt caaaagtatt taaccgagta atcaaatgat caaatgaagt tggataaagc
    62401 gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg ttgttttcat taaatcactg
    62461 aaaggaaatg cttctgcgtt tccttttttc tttttgttgt gcgctgtata ccttgaggat
    62521 attcactggt ggcgtacact taaatagtaa tatattgtgt tactttatgt ctgtggtaat
    62581 agttatagtt gcttaccaat gtatgttcat ttttcgaaat gttgacttca taatccttgg
    62641 cttaatgttt gaattttttt ggtgatagat tttttcagcc gtataaaata aatcgtaatt
    62701 ctattataga gagttgtgat tataaaaaga tagcaagaaa aaaaagaaag tttctataca
    62761 ttttaaatat tttattccta tttctattgc taaaaaagta gaaattctat atttaccttt
    62821 aactctcgct tctcttgcaa atgtagttag cattttaatc gataataaac tcaggtagtt
    62881 atattacctt gaaatgattg ataattttgt cacttctatt ttttttgcaa taaat
//
LOCUS       DACDLI010000008        59658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDLI010000008 DACDLI010000000
VERSION     DACDLI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.008"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IV353_000546"
     CDS             complement(451..1761)
                     /locus_tag="IV353_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9008820.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IV353_000547"
     CDS             complement(1773..3191)
                     /locus_tag="IV353_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9008821.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IV353_000548"
     CDS             complement(3431..4999)
                     /locus_tag="IV353_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9008822.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IV353_000549"
     CDS             complement(5003..5413)
                     /locus_tag="IV353_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9008823.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IV353_000550"
     CDS             complement(5410..6333)
                     /locus_tag="IV353_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9008824.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IV353_000551"
     CDS             complement(6330..7208)
                     /locus_tag="IV353_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9008825.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IV353_000552"
     CDS             complement(7195..8532)
                     /locus_tag="IV353_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9008826.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IV353_000553"
     CDS             complement(8535..9638)
                     /locus_tag="IV353_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9008827.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IV353_000554"
     CDS             complement(9712..11220)
                     /locus_tag="IV353_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9008828.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IV353_000555"
     CDS             complement(11186..11878)
                     /locus_tag="IV353_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9008829.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IV353_000556"
     CDS             complement(11900..12874)
                     /locus_tag="IV353_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9008830.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IV353_000557"
     CDS             13174..14046
                     /locus_tag="IV353_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9008831.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IV353_000558"
     CDS             complement(14504..16702)
                     /locus_tag="IV353_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9008832.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IV353_000559"
     CDS             16934..17926
                     /locus_tag="IV353_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9008833.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IV353_000560"
     CDS             17986..19407
                     /locus_tag="IV353_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9008834.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IV353_000561"
     CDS             19400..20899
                     /locus_tag="IV353_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9008835.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IV353_000562"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IV353_000562"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9008836.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IV353_000563"
     CDS             22491..23174
                     /locus_tag="IV353_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9008837.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="IV353_000564"
     CDS             23176..24012
                     /locus_tag="IV353_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9008838.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="IV353_000565"
     CDS             24023..25777
                     /locus_tag="IV353_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008839.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="IV353_000566"
     CDS             complement(26053..26394)
                     /locus_tag="IV353_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9008840.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="IV353_000567"
     CDS             complement(26462..28633)
                     /locus_tag="IV353_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9008841.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="IV353_000568"
     CDS             28844..29701
                     /locus_tag="IV353_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9008842.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="IV353_000569"
     CDS             complement(29772..30629)
                     /locus_tag="IV353_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9008843.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="IV353_000570"
     CDS             complement(30614..33316)
                     /locus_tag="IV353_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9008844.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="IV353_000571"
     CDS             complement(33329..34618)
                     /locus_tag="IV353_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9008845.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="IV353_000572"
     CDS             34768..35814
                     /locus_tag="IV353_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9008846.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="IV353_000573"
     CDS             35816..37969
                     /locus_tag="IV353_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9008847.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="IV353_000574"
     CDS             complement(38442..39893)
                     /locus_tag="IV353_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9008848.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="IV353_000575"
     CDS             complement(39890..41614)
                     /locus_tag="IV353_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9008849.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="IV353_000576"
     CDS             complement(41607..42221)
                     /locus_tag="IV353_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9008850.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="IV353_000577"
     CDS             complement(42566..44023)
                     /locus_tag="IV353_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9008851.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="IV353_000578"
     CDS             complement(44064..44984)
                     /locus_tag="IV353_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9008852.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="IV353_000579"
     CDS             complement(44996..45943)
                     /locus_tag="IV353_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9008853.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="IV353_000580"
     CDS             complement(46422..47141)
                     /locus_tag="IV353_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9008854.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="IV353_000581"
     CDS             complement(47190..48836)
                     /locus_tag="IV353_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9008855.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="IV353_000582"
     CDS             49015..49605
                     /locus_tag="IV353_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9008856.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="IV353_000583"
     CDS             49697..51202
                     /locus_tag="IV353_000583"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9008857.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="IV353_000584"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="IV353_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9008858.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="IV353_000585"
     CDS             complement(52638..53156)
                     /locus_tag="IV353_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008859.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="IV353_000586"
     CDS             complement(53172..53498)
                     /locus_tag="IV353_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9008860.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="IV353_000587"
     CDS             complement(53511..55490)
                     /locus_tag="IV353_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9008861.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="IV353_000588"
     CDS             complement(55511..55825)
                     /locus_tag="IV353_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9008862.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="IV353_000589"
     CDS             55993..56286
                     /locus_tag="IV353_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9008863.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="IV353_000590"
     CDS             complement(56364..57476)
                     /locus_tag="IV353_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9008864.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57863..58042
                     /locus_tag="IV353_000591"
     CDS             57863..58042
                     /locus_tag="IV353_000591"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008865.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58236..58406
                     /locus_tag="IV353_000592"
     CDS             58236..58406
                     /locus_tag="IV353_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9008866.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58830..59570)
                     /locus_tag="IV353_000593"
     CDS             complement(58830..59570)
                     /locus_tag="IV353_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9008867.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17827 a        12195 c        10432 g        19204 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    59281 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    59341 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    59401 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt
    59461 ccattggcat cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg
    59521 atctttgtca acacgacacc gcctggtgta agtcggtttg ctttcgtcaa ttctaccacc
    59581 gctgtctgac ttctttcgat ctcaaactcg atcggtgtcg catctaattc ataccccgtt
    59641 ggcgcttgag tttctacc
//
LOCUS       DACDLI010000009        58824 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDLI010000009 DACDLI010000000
VERSION     DACDLI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.009"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IV353_000594"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IV353_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9008868.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IV353_000595"
     CDS             complement(740..1462)
                     /locus_tag="IV353_000595"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9008869.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IV353_000596"
     CDS             complement(1598..2479)
                     /locus_tag="IV353_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9008870.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IV353_000597"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IV353_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9008871.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IV353_000598"
     CDS             3719..4198
                     /locus_tag="IV353_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9008872.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IV353_000599"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IV353_000599"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9008873.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..6284
                     /locus_tag="IV353_000600"
     CDS             5499..6284
                     /locus_tag="IV353_000600"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9008874.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6630..8321)
                     /locus_tag="IV353_000601"
     CDS             complement(6630..8321)
                     /locus_tag="IV353_000601"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9008875.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8743..9690)
                     /gene="arcC"
                     /locus_tag="IV353_000602"
     CDS             complement(8743..9690)
                     /gene="arcC"
                     /locus_tag="IV353_000602"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9008876.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9805..10824)
                     /gene="argF"
                     /locus_tag="IV353_000603"
     CDS             complement(9805..10824)
                     /gene="argF"
                     /locus_tag="IV353_000603"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9008877.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10915..12144)
                     /gene="arcA"
                     /locus_tag="IV353_000604"
     CDS             complement(10915..12144)
                     /gene="arcA"
                     /locus_tag="IV353_000604"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9008878.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12605..13306
                     /locus_tag="IV353_000605"
     CDS             12605..13306
                     /locus_tag="IV353_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9008879.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13878..14894)
                     /gene="tsaD"
                     /locus_tag="IV353_000606"
     CDS             complement(13878..14894)
                     /gene="tsaD"
                     /locus_tag="IV353_000606"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9008880.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14891..15355)
                     /gene="rimI"
                     /locus_tag="IV353_000607"
     CDS             complement(14891..15355)
                     /gene="rimI"
                     /locus_tag="IV353_000607"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9008881.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15362..15904)
                     /gene="rimI"
                     /locus_tag="IV353_000608"
     CDS             complement(15362..15904)
                     /gene="rimI"
                     /locus_tag="IV353_000608"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9008882.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15888..16712)
                     /gene="tsaB"
                     /locus_tag="IV353_000609"
     CDS             complement(15888..16712)
                     /gene="tsaB"
                     /locus_tag="IV353_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9008883.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(16801..17781)
                     /locus_tag="IV353_000610"
     CDS             complement(16801..17781)
                     /locus_tag="IV353_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9008884.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17805..19289)
                     /gene="galT"
                     /locus_tag="IV353_000611"
     CDS             complement(17805..19289)
                     /gene="galT"
                     /locus_tag="IV353_000611"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9008885.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19301..20290)
                     /gene="galE"
                     /locus_tag="IV353_000612"
     CDS             complement(19301..20290)
                     /gene="galE"
                     /locus_tag="IV353_000612"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9008886.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20539..20706
                     /locus_tag="IV353_000613"
     CDS             20539..20706
                     /locus_tag="IV353_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9008887.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20768..22579)
                     /locus_tag="IV353_000614"
     CDS             complement(20768..22579)
                     /locus_tag="IV353_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9008888.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22576..22941)
                     /locus_tag="IV353_000615"
     CDS             complement(22576..22941)
                     /locus_tag="IV353_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9008889.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(23104..23499)
                     /locus_tag="IV353_000616"
     CDS             complement(23104..23499)
                     /locus_tag="IV353_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9008890.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23517..24479)
                     /locus_tag="IV353_000617"
     CDS             complement(23517..24479)
                     /locus_tag="IV353_000617"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9008891.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24479..24691)
                     /locus_tag="IV353_000618"
     CDS             complement(24479..24691)
                     /locus_tag="IV353_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9008892.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24712..25410)
                     /locus_tag="IV353_000619"
     CDS             complement(24712..25410)
                     /locus_tag="IV353_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9008893.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25430..25972)
                     /locus_tag="IV353_000620"
     CDS             complement(25430..25972)
                     /locus_tag="IV353_000620"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9008894.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(26104..27108)
                     /locus_tag="IV353_000621"
     CDS             complement(26104..27108)
                     /locus_tag="IV353_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9008895.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(27105..28094)
                     /locus_tag="IV353_000622"
     CDS             complement(27105..28094)
                     /locus_tag="IV353_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9008896.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(28091..28897)
                     /locus_tag="IV353_000623"
     CDS             complement(28091..28897)
                     /locus_tag="IV353_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9008897.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            29063..30019
                     /locus_tag="IV353_000624"
     CDS             29063..30019
                     /locus_tag="IV353_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9008898.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(30096..30614)
                     /locus_tag="IV353_000625"
     CDS             complement(30096..30614)
                     /locus_tag="IV353_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9008899.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(30702..30851)
                     /gene="rpmG"
                     /locus_tag="IV353_000626"
     CDS             complement(30702..30851)
                     /gene="rpmG"
                     /locus_tag="IV353_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9008900.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            31079..31525
                     /locus_tag="IV353_000627"
     CDS             31079..31525
                     /locus_tag="IV353_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9008901.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(31720..33615)
                     /gene="asnB"
                     /locus_tag="IV353_000628"
     CDS             complement(31720..33615)
                     /gene="asnB"
                     /locus_tag="IV353_000628"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9008902.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(33940..34914)
                     /locus_tag="IV353_000629"
     CDS             complement(33940..34914)
                     /locus_tag="IV353_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9008903.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(35250..35337)
                     /locus_tag="IV353_000630"
     tRNA            complement(35250..35337)
                     /locus_tag="IV353_000630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35303..35305),aa:Ser,seq:cga)
     gene            complement(35482..36048)
                     /locus_tag="IV353_000631"
     CDS             complement(35482..36048)
                     /locus_tag="IV353_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9008904.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            36303..37634
                     /locus_tag="IV353_000632"
     CDS             36303..37634
                     /locus_tag="IV353_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9008905.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            37600..37950
                     /locus_tag="IV353_000633"
     CDS             37600..37950
                     /locus_tag="IV353_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008906.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(38042..38461)
                     /locus_tag="IV353_000634"
     CDS             complement(38042..38461)
                     /locus_tag="IV353_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9008907.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(38462..38806)
                     /locus_tag="IV353_000635"
     CDS             complement(38462..38806)
                     /locus_tag="IV353_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9008908.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            39156..39752
                     /locus_tag="IV353_000636"
     CDS             39156..39752
                     /locus_tag="IV353_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9008909.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            39876..41675
                     /locus_tag="IV353_000637"
     CDS             39876..41675
                     /locus_tag="IV353_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9008910.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(41953..42165)
                     /locus_tag="IV353_000638"
     CDS             complement(41953..42165)
                     /locus_tag="IV353_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008911.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            43044..43430
                     /locus_tag="IV353_000639"
     CDS             43044..43430
                     /locus_tag="IV353_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9008912.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(43738..45084)
                     /locus_tag="IV353_000640"
     CDS             complement(43738..45084)
                     /locus_tag="IV353_000640"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9008913.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(45196..46545)
                     /gene="gdhA"
                     /locus_tag="IV353_000641"
     CDS             complement(45196..46545)
                     /gene="gdhA"
                     /locus_tag="IV353_000641"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9008914.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            46662..47915
                     /locus_tag="IV353_000642"
     CDS             46662..47915
                     /locus_tag="IV353_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9008915.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            complement(47986..48471)
                     /gene="ybaK"
                     /locus_tag="IV353_000643"
     CDS             complement(47986..48471)
                     /gene="ybaK"
                     /locus_tag="IV353_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9008916.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(48494..49252)
                     /gene="aroD"
                     /locus_tag="IV353_000644"
     CDS             complement(48494..49252)
                     /gene="aroD"
                     /locus_tag="IV353_000644"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9008917.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            complement(49268..50446)
                     /locus_tag="IV353_000645"
     CDS             complement(49268..50446)
                     /locus_tag="IV353_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9008918.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            50676..52796
                     /locus_tag="IV353_000646"
     CDS             50676..52796
                     /locus_tag="IV353_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9008919.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            53019..53744
                     /locus_tag="IV353_000647"
     CDS             53019..53744
                     /locus_tag="IV353_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008920.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            53734..54243
                     /locus_tag="IV353_000648"
     CDS             53734..54243
                     /locus_tag="IV353_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008921.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(54313..55761)
                     /locus_tag="IV353_000649"
     CDS             complement(54313..55761)
                     /locus_tag="IV353_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9008922.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(55761..56477)
                     /locus_tag="IV353_000650"
     CDS             complement(55761..56477)
                     /locus_tag="IV353_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9008923.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(56458..56808)
                     /locus_tag="IV353_000651"
     CDS             complement(56458..56808)
                     /locus_tag="IV353_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9008924.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            56952..57725
                     /locus_tag="IV353_000652"
     CDS             56952..57725
                     /locus_tag="IV353_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9008925.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            57896..58262
                     /gene="ssrA"
                     /locus_tag="IV353_000653"
     tmRNA           57896..58262
                     /gene="ssrA"
                     /locus_tag="IV353_000653"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(58567..>58824)
                     /locus_tag="IV353_000654"
     CDS             complement(58567..>58824)
                     /locus_tag="IV353_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9008926.1"
                     /translation="SISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMDFHQ
                     VNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT        17129 a        11951 c        10298 g        19446 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttaatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgctcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccacccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgca tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattaa
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaattttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa ctcctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcggc tcctttgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatgg
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccctctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaaaagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgattcgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactaga
     5041 aaagcgaaaa aatgtgagtc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtactac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa cggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgcgaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gactggattt actgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaagggta ttcaccagac ttcatggcaa
     5941 cttctgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaaagat cgtttaaaac
     6181 agtctgttgc tcttagattc caacaagctg gcgctgatat ttgtgtaaac aatttaaaag
     6241 agttaacaga atacattttg tacgctgaca agaatccaga atgactttta tacaaaatca
     6301 gaggtcgtga aaaagctgta ctgcatcaag taataagaac agccaaacaa aaatagctcc
     6361 tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat
     6421 tcgaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tacaaacaaa
     6481 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6541 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6601 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6661 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6721 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6781 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6841 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6901 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6961 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     7021 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     7081 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7141 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7201 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggcg catatcgtca
     7261 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7321 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7381 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7441 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7501 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7561 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7621 tcacggaacc attgccataa ttgaatagcc tcttcatcgc cttcttccag acgtttgaac
     7681 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggaca
     7741 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7801 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7861 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7921 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7981 gttccttgat tcccgatatt gctatcccca taatgttctt tttctttcac tactgtttgc
     8041 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     8101 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8161 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8221 gctgattttg gattttctag taattgttcg acttgttcca gtgttaaatc atctttgaca
     8281 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8341 ctcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8401 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8461 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8521 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8581 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8641 gctagtcgtt tctgataaaa aaacaatcca gatggcctaa aggagaagaa ataccacctg
     8701 gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag ctacgataat
     8761 cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga tgattgcctt
     8821 agcgtttggt tttgcttcaa caaatgcaat agctgcctct actttcggaa gcatgcttcc
     8881 tggagcaaat tgcttttcat caatgtattg tttcatttct gaaacagtaa cagtttctaa
     8941 tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg tcaatacgat
     9001 cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt tatcgattac
     9061 ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac cgccaccaac
     9121 agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt ctttaatcga
     9181 gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt ctttgaatgt
     9241 cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga atggaccgac
     9301 tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt gagtgactaa
     9361 agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg cattttgtaa
     9421 ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca ttgccggtgt
     9481 tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg gaccatttcc
     9541 gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact ttgctgtttc
     9601 cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagattg cattaccccc
     9661 taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag ttctaccgaa
     9721 ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct gactcgtttt
     9781 ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac ctaatgttgc
     9841 tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt gacgagcata
     9901 tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc gttctttcat
     9961 ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca agaagatcaa
    10021 acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag gtttcaacaa
    10081 tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat cagaataaag
    10141 gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt gtgcaccgga
    10201 tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta attcttttgg
    10261 tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag agttagccat
    10321 attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc caaagttttc
    10381 ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag tcaagccatt
    10441 ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac tgaatccacg
    10501 gaattcgatt ccatcaaaca tacgacctag tactttagct gtatcttctg tggattcttt
    10561 tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta gatcgatcgc
    10621 tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa gcgcaatatt
    10681 ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca agtgttctga
    10741 aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta ataagcttct
    10801 tccttggaat actgattctt tcatattaaa cattctcctt tagattgttt ttatttaaga
    10861 ttaaagagga aatcccgacg gcagctgaca gtaagaccgc cgtcaggaaa caaattactt
    10921 caagtcttca cggacaagtg gttggctcat gcagcgtgga ccaccacggc ctcgtgacaa
    10981 ttcacttgaa ttgatttcta acactttgat gccatagcta cgaagcaatt cgttcgatac
    11041 ataatttcgg ttataagtga ctacaacacc tggagcgatt gccaatgtgt ttgatccatc
    11101 attccattgt tctcgaggtg caacgatctc atctccatta ccagttggga tcagtacaag
    11161 atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag ttatctgaat gagtaatctt
    11221 gatatcatca ccatctggtt cgattgtaaa gacatccatc ttaccgtctt tactttgaat
    11281 tgctggatgg atagtgaatt tatcgtgatc taccattgta aatacggtat ccaaatgcat
    11341 cattgcacgg ttgtttggaa ttttgattgc taacactttt tcaaatcctg aattttttgc
    11401 gaataggtgg cgtgctaatt tttctaaagc tttcgcatta gttcgttgag aaatcccaac
    11461 agctactact ttatcgctta agatcaattc gtctccgcct tcgatatgat ctggattttc
    11521 acgatccaac catacttcga ttcctttatt tgcaaatctt ggatgatgtt tcaaaatata
    11581 ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc atcttattga ctgtcatccc
    11641 atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag tttggcattg ggtccatgta
    11701 aaatggatag tcatcgtctg ctgacagatc gtatagactg ctagaacgta cttcaatatc
    11761 ttttgtacga accccagcca tgattttatc taccatttct tgcgtttcca ttgatagaag
    11821 gaactcatga agaccctctc ttacagcatt cgatgcaata tgagattcat ccaacatttt
    11881 attcaagaat tgttctttga cattgcctgc gtcaatcgct tctgccgcta atttttccaa
    11941 gtaaagagtc tcgacgcctt cattttgcaa tgtctttgca aagttgtcat gttcttcttg
    12001 agcaatcgga agatatggaa tatcatcgaa caacagacga tccataatat ctggtgtcaa
    12061 attttccact tcttgtccag gccttttcag taaaactgtc tttaatttcc ctatttcaga
    12121 gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt ctttttgatt acatgtatag
    12181 gatatcctcc ttttgtaatc actttcataa ttttgtctca ttcaaaatga gacattcctc
    12241 acattaatat tcgataatta aataattaaa cgtttttcta tttttaagcg taatttattc
    12301 attttttatt ctgtatacac tttcaaaaaa atatttattc attgaaaaaa attttttgaa
    12361 taaattaaaa aaataaatta tattagcgtt gttagcgttt actatatgta ttattgttgt
    12421 tttttataca caaatcattc gcttcaaaac aaattattca ataccattgt atatttttta
    12481 cataataaca aattatattt gtaaagtatt aaaacttgtt tctaatgttg gtatgttaaa
    12541 actttacaaa ttctttaaaa aaagtgacaa taaatatagt gaacaataag gagaggactt
    12601 tttaatgcgt gttaataaaa tcgattttca cttttcaaaa ctcagatatc aacctgattt
    12661 tgaacatttc tcggatgaag agttccaaca gatcaaggaa catactgttc ttcgttctta
    12721 taaaaaagga cagatccttt tcgacgatgg tgatcccaga caacggttgt atatcttgac
    12781 caaaggactc gttcgtttag aacgttatga tgaaagtgca acttattact actatgacta
    12841 tgtgaataca aataaccttt ttcctatgtc aggaatgttt ggagaagaga cctacactta
    12901 tacagctcaa gcgatgactg atattgaaac attctacctt cctgtttcct actatgaaaa
    12961 attagcacga aaaaatcccg aacagttgat ctatatgata caaaaaattt ctaaagtagt
    13021 tgaaatacat gaattgcgta tccaaatcgg attgacctct agcgcctttg accgggtaaa
    13081 acaaaattta ttcattttga aagaagaatt gggcgtacca acaaaagatg gtcatatcac
    13141 cattccttat ccgatcacct taaaagaatt agcagtgaat agcggcacga cacgtgaaac
    13201 tgctggacaa gtaatcaaac aactacgtga ttccggactg ttagattatg acaaaaagcg
    13261 ttttactttc tgtaagacag ctactactcc agctatgaga gaataacgcg aacaaaaata
    13321 agcagtgacc ctgatattta tcagggtcac tgcttatttt cctccctaaa acttctgaaa
    13381 gatccggcag ttttagatca ctgggcaaga ctcaaagatt ttctatgtgt gtttcagtta
    13441 tgtaatattc actgtttgca aaaaatactg atagattaac tagaggaagc gtcttgtcct
    13501 catccgacct agattattcg atattcctcg acaataaacg attaatcagt gagggcaatc
    13561 agaggaacgg ataatgatga ataagctgta aaaaataggg ctttgacaaa agcgtgtcac
    13621 agcccttatt tcgattaaac ggtgtgtacc tgtcaactct tcggtcacaa gtcataatgt
    13681 tcataaacat gaggagctgt tttttgaaat tgttccttgt gaatcagagt taaacgacag
    13741 gcttacgaac tcttgattaa acggcgttca aaagcttgca tacgcggtaa taagaacagc
    13801 caggtcaaaa acatgagaag ccgtttttcc tgggctgttc ttattacttg atgcagggca
    13861 gatttttcac aacctcttta ttcagataaa accaagcttg gatcagcatt caactgatag
    13921 ctagcaaaat ttcttttttg catttccaca tgtgctgctg ctccaatcat tgcagcatta
    13981 tctccacaaa gtcgaagagg gggaatcact agttctacct cagggagttt agttgccaaa
    14041 gcatcctgca atccttcacg caacccttga ttggccgcta cacctccagc tacaatcagt
    14101 tgcttgactg gataattttg acaagctcga agtgttttag taatcaatac atcgatcaca
    14161 cttgcttgaa agctagctgc taaatcattt ttgtctagat cttctccacg ctgttgtgca
    14221 ttatgcacca aattgataaa tgcacttttt aatccgctaa aactgaaatc ataattatct
    14281 tcatggatca tcgctcgtgg aaaatggtaa ttatcttttc cctgatgagc tagctgatcg
    14341 atttcttttc cgcttgggta agaaagacct aatactcttc caactttgtc ataagcttct
    14401 cctgctgcgt catctcttgt ctccccaatg atttcatatg atccatcttc ttgcatatag
    14461 actaattctg tatgtcctcc gctaacaaga agtgccatca atggaaactg gaatggttta
    14521 accagacggg ctgcatagat atgccctgcc atatggttta caggaataag cggcaattgg
    14581 tgtgcccaag caaaagcctt agctgcagaa ataccaatca gcaacgagcc gacaagtcct
    14641 ggtccataag tgacagcgac tgccgataaa tcttctgccg acacaccagc ttctaccagt
    14701 gcatcttcca agcataaggt gatttgttct acgtgatggc ggctagctac ttcaggaact
    14761 acgccaccaa aacgtttatg actgtttatt tgagaagcaa cgatgttaga taagatctct
    14821 gttccatttc taacgaccgc tacactcgtt tcatcacaac ttgattcaat cgccagaatc
    14881 aattcttcat tcatttcatc gtctccgttt ttaactttct gctcataatg atcgcatctt
    14941 ccgtcggatt gcgataatat gatttccgtt tacctaatat acgaaacttt tctgattcat
    15001 aaagttttct tgctgcttca ttagatagac gtacttctaa aaaaacatgc agcgaatctt
    15061 ttatttgttc cgtaatcagt aattgtaaca aatgccgtgc atgccctttt ccttgttctg
    15121 aaactgcaac agcaatattt gtgatctcga cttcatctat tactttcgag tatccaataa
    15181 agccacagat ctggttatct tcaactaaaa gcagataatc agagtgaggc tgttggatgt
    15241 ctgccaaaaa ctgcattttc gtccaaggtg aaccatgtgg ataagcattt tcgctaatca
    15301 tccatacgca ttctgccaag tacgcttcat caaaatcttt ttttctgcaa atcaactaat
    15361 ctcactcatt ttctaaaagt tttatcatat ccaacgcatc ttcttttgtt tcagtgtaat
    15421 atcttttttt gatcgttgtt gcttgaaaac ctaacttccg ataaagtctt tgtgcatctt
    15481 gattactcat acgcacttct aatgacatcg tctcacagcg attcattata gcaaagttct
    15541 cgatttcgtc aataagcagc gaaccgattc gtttacgctg atatgccggt cgaacggcaa
    15601 tattcgtgat atggcaatct aaaccgaacg ctcttgaacc gataaaacca atcattcttc
    15661 cattatcaaa gatacctaga tatagatgag gaagcggtga cttcaattct gatataaagg
    15721 cactttttgt ccatggcaat tcgccattat agacttctcg ttcgacatcc agcagctctt
    15781 tgatatcttc tgtagtcaat tctcttagta cgtaacgacg atcgatttgt tcgattgttt
    15841 tttctgtata aatcatttta tcttgaaaaa agcttctggc atacgctcta aattttttca
    15901 acataacttt cgtctccagg tgtgtgcgtc tccaaccatt tttcttctgc ttctacccgt
    15961 tttaaatagc gaggaagaaa agcttgtttc tctgcaactg gttcagctaa agcacctaat
    16021 tgagccacag ttacaccact tggaatatcc catgctggaa ctgtattaat catcgcacta
    16081 ggcaatattt cctttatttc atagaagaat ttgtgacaat cactcccgac gaaataaata
    16141 gcttcattca gcttttgtag ttgtgtgaac aaatcagaag cagaaatatg tacatcttca
    16201 agtacaattt ctaatctatt ttctttccag cggtatgccc cagcgtatac attttttctt
    16261 cgagcatcaa acatgggcac aataatttta tccacaccga tgcaattagc cgcgatggtt
    16321 ttcagactag atactcctac tagctcttta tctaatgtat aagcaatcgt ttttgctgtc
    16381 gttacaccta tacgaagccc tgtataagaa ccgggaccat cagaaaccgc gattcggtca
    16441 atgtcttttg gacttatttg aagatctgca aacaattggt cgattgcagg catcagtgtt
    16501 acactgtggt tgcgtttgat atttgtttgt atttgtccaa gaatttgttc gtcttctacc
    16561 acaccgatcg ccaaagtttg gtttgcagta tctattccta atgtaatcat atatttgatc
    16621 ctttctgcta tgacattctc gccttccatt gtagcatact tttttattct gaggatgaaa
    16681 ataccaaagg caaaactagt acaatgatac atgattatct gctggcgggc aaattttgcc
    16741 ccttcctgaa acacaaaaaa gctgcggtat cacttcattt ccccacagct tttccatctt
    16801 ttatttcgtt gattctcgaa taatcaactc tgtttccaca ataatctttc ttgaaaccgg
    16861 tgctttggaa ttacttaatt gaagaatcgt ctccactgct agctctccca tccattcggt
    16921 ataaactttt acagtagaaa gagagggcgt tacatatttc gctacactga catcattgaa
    16981 acccatcaca gaaatatctt ctggtaccac taaacctttt tcttgaatcg ctttcagtgc
    17041 gccaatcgct aatgcatcat tagctgcaaa aagcgcatca ggaacatgcg gattcgtttt
    17101 taaatactgc ttcactgctt gatatcctga ctcgacagaa aaatctgctt cgataattgg
    17161 accttcgtcg tagccttttt ctgccaaaac ttgttgaaat gccataattc tgggatcttt
    17221 tagccgctga tggtttttct tcgtatattc tgttccagca agcatagcga tatgttgcgc
    17281 tttatgggag acaaaatgtt caatgacttt ttgaacactt gacttaaagt cgacaactag
    17341 agagtcgatt ccttgagaag tgccgtcaga atctaccaaa agcaaggcat tttgggatcc
    17401 tttcatttct aataactgag cttcgtcgaa ttttcctagt gcgatcgttc catctgtcgg
    17461 aagatcacta atggaatccc acgtttcttt caataaagta acgtttgctt cttctgcctt
    17521 tttttcgatt cctagacgaa tagataaata ataaagatct tcaagctctt ccgtatcgtc
    17581 gtaccactgg atcaggcgaa gcgtttgctt agtatttgct gcatttttac gatgtttcgt
    17641 ataatttaag cgttctgctg cttcaaaaat tttttgcttt gtggctaagc caacggaaag
    17701 atcaggatca tagttgagaa ctcttgaaac agtagcagga gaaactcctg ccaactgcgc
    17761 aatgtcttta attgttgcca tttgtctctc ctcctttttt acgtttataa aattgcagta
    17821 aatcgttcaa acgctgcctg tcctttttca tctcgcttga atactccagc gtcttccagt
    17881 acacgtgcaa aaacctgacc tacagatttt tgtacgacat cggtgaccgt atctttcgtc
    17941 aaatcaggat gttctttttt taattgttct gcccacggac gatgatattc agccatctgg
    18001 ttttcttctc ctaataagaa atgctctact tctactagtt ctggtttcaa acgaggtggt
    18061 aaaacagcaa gccccattac ttcgatcaac ccaatgttct cctttttgat atgttggaca
    18121 tctttatgtg gatgaaaaat cccatcagga tgttcttctg aaacattgtt gtctcgaagg
    18181 acaaggtcca actcaaagta attgtccctt cttcgtgcaa ttggcgtaat cgtatggtgt
    18241 ggtgtaccat ctgctgaaaa agctctgata gatactgttt catccgaata tgctttccac
    18301 ttttccaata ctgagttagc tgcttcaatc aacaattttt tctctgttga ttgcaaacgg
    18361 atcacagaca tcggccattt cacgactcct gcgatgactt gcggataatc tgctagtttg
    18421 ataagttttt ctatcggtgc ttttgccata ggaaattcat gacgtcctgc ctgataatga
    18481 tcatgagaaa gaatcgatcc tccaacaatc ggcaagtcag cattcgaacc aacaaagtaa
    18541 tgaggcaaga cttcagtaat ccgaagcagt ctagcaaatg tttcctttgt gattttcatc
    18601 gggcggtgtt gttctgataa aatgatcgaa tgttcattgt aataagcata aggagaatat
    18661 tggaatcccc agctttcacc gtctagattc attcgaataa tccggtgatt ggtgcgggct
    18721 ggatagttca agcgacctct atatccttcg ttttccatgc aaagcatgca ggtaggatag
    18781 tctactttta ccgcctcgcg ttctgctgcg atttgttttg gatctttttc tggcttagat
    18841 aaattgattg tgatttccag ttcaccatac tccgactctg ccggaaattt gatgtttttt
    18901 gcgatggctc ttgttttgat gtagtcattt ttcttactta aattaaagaa atactcagtt
    18961 gcttcctctg ggcttttcga atagtgctgt gcaaagaatg cattgacgac tgaaggtggc
    19021 ggtgtgagaa aatccattaa ttgcgcttca aaaatctctt gttctgctgg caattctgaa
    19081 agaacaccat tttctcttgc cttagcgaca agctgatcca ataagtctac agattcttcc
    19141 ttaactggtc ttacttcaat gctttctaga gcatcttctc ctaccatccc taaaagacga
    19201 ttctgtaaat ataagcggtc catttccatc catccgccag cttcgatcgc aagtgtggca
    19261 aaatcacaaa tcgtttgact gattgacata tctcattccc ctattcttca taaccatttg
    19321 gatggctgac atgccaatcc catgctgttt tgataattgc tttgatatct gtgtattcag
    19381 gtttccaacc cagaacctct ctcgcttttt cacttgaagc gactaatcgg ctaggatctc
    19441 cagctcttct tggtgctatt tctgctggaa tttcttttcc tgtcacttct ctagctgctt
    19501 ctagcatttc cttgactgag tatcctttgc tgctgcctaa attgaagaag ttgctttcat
    19561 ttccttcttt caagtattcc aaagctaaaa tatgtgcagc aatcaaatct tctacttgaa
    19621 cataatctct gatacatgtt ccatctggtg tatcataatc gtctccataa acagccaatg
    19681 cttttctttg accaagagct acttgtaaaa tgattgggac aagatgcgtt tctggtgtat
    19741 gatcttctcc gatagatgca tctgcttttg ctccggctac attgaagtag cgcaaagcga
    19801 catagcgcat gccatatgct tggtcacacc acttcatcat tttttccatc atcaatttac
    19861 tttcaccata cggattttta ggattagctg gtgtatcttc tgtgattgga gactcttttg
    19921 gttctccgta agttgctgca gtagaagaga acacgatttt attcacattg aactcgtgca
    19981 tgacttcaag taagatttgc ataccgtata cgttgttgtt gaaatacatc aatggttttt
    20041 cgacagattc tcctactaaa gagcttgcag caaaatggat tactccttca atcggttcct
    20101 tttcaaatac ggaacgtaga aattctttat cccggatgtc tccttcataa aaatgagcat
    20161 ctggatgtac tgcttgtttg tgacctgtga gcaaattatc taccacagct acttgatacc
    20221 ccttttgaac tagctggtct acagcgtgcg aaccgatata accggctccg cctagtacta
    20281 aaattgtcat aaaaaacact cctttgatta gtaaaattgt gtttgttgat tatatataat
    20341 ttaaaaaaac gaagtttact tttatggtga aaacaaacaa agtaaaattt cgtaaattca
    20401 tatggtaaat ttataataaa acatttagta aacattttca aggatttttt gcaaaggttt
    20461 acaaacaaac gtaaaactag cctttttttc tcttttgttc cataatagat ataacaaata
    20521 ataaatgagg tggattcaat gaaaaaattt gtatctggaa tattagtagg aagtctggca
    20581 acagccgctg ccgttgctgg tttggtcgct tctgtgaaaa aaacagtgat cgatccaatt
    20641 gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag caatgcgcaa acgcgtttct
    20701 cgttaataat tatttaaaaa cacgcatgaa gacttgtgaa agaacagata agtcatcatg
    20761 cgtattttta tttgtcagtc aatataagtt gtgctatttt atctgcctgt ctcagattcc
    20821 atggatctgt ataaggtcta gctactgctg cagttagcca atccacacct tcaacaggta
    20881 ttccatagca ataaaagtca gactggattt cccctgaagc atcaatcacg cgattcgtac
    20941 ctcgctcaac atctatagct tctgaatgat aacttcctgt attatctgaa aatgaaaaag
    21001 ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg attacttgaa cgagaaaagc
    21061 ttgtttgtgg caatcgagct tcaatcaaat agcgactgct gatttcttta tcttgtagag
    21121 aaataacata gcggttgtgc ttttgctgaa tatcaaatgg cggttcgaac aaatgaaaaa
    21181 tacccgcatc tatcagtgct gctaattctt tcgtccgaat gactggcgga ccgatcgtta
    21241 aaaagctatt tagatgagta aaccatcccc atagtatttc tttgtattcc tttgcagaaa
    21301 aaacgtgcca ttcaatagcc tgccggattg gatcacgcca gtcttttaat gcatcaaatg
    21361 ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc ttttatctgc ccttcaataa
    21421 atgctcggct agcttctgca aaggactcat cctgataggt aagaggaatt tctaaatacg
    21481 accaatccca tctataaggg attagtgctg gatgctttcc gagacactcc tctgaagaga
    21541 gggagagaaa atcatgctca aaatttttct ttggaatcgg caaatgatat tcttcgatga
    21601 ttttcttgta ataaaacaac tcaacgtctt ttttcagata gtcaaaaaat gtttcgccag
    21661 tcaaaaatcc cgactgatgc cattgctcca ggttttcttt tgttagtaac cgtggccatg
    21721 ccattgatcc gccttgcttt tggtttcttc ctcgagggta ataagggagc ccccttcttg
    21781 atccactgta tacaatcggt tccttgcctg atggatgata gacaagtttt cctttttcgt
    21841 tctcgaagcg cccttctctt gatactgtaa acaatccgac ataatcaaaa aaacttaagc
    21901 ctagacctct taaaaagaca ttttctttag gcggtatctt cgaaagatcg acatctgcag
    21961 gattagaagg aagctgatac agtaaatcat ttttctttgc gtagtctacc cacactcttt
    22021 cttcttctgt ctcatcattt tcccagtgac cgcttgctaa aacaagctga tcgatagaat
    22081 agctttgctg atctgtctgt aaaagataac catcttttct tttgctgact ttttttacta
    22141 aagaataaac aaactgaata gaagctggaa attcttcttg caactttgag aaaaaccagc
    22201 gttgatagag tccgtataaa catcgagtag attgctgatc tttttccaga cgattgattt
    22261 cttctataat tagttgctta tttttggtta ccgtctgttc gatataatct gtagcaggtc
    22321 ctttactcca ttgaaacaag ttgggtccct gtgttaccaa tcctccactg gacaatgttt
    22381 catctgtaaa aagcgtcact tgttgagaaa cagaattcat taataactcc ggtggctgat
    22441 tcaaccgcca aactcgacct ccaggaccat tgggatcgaa aagaaaaagt tcaagagaat
    22501 gtctgtcctg attattatgg agaatccttt ctgctatact taatcctcta ggtccggcac
    22561 caatgatccc tattttcatt tgcatgcctc catttcgata aattttttca ataaaacagc
    22621 atggttctga tgttcatttt ttgcaccata aacaaaagta atgactggat gttctttcat
    22681 aagttgactg agtttaaaaa tagcttgctt cttttcttct tcgaaaatca attcttgctg
    22741 gtatttcttt gcaaatgaag caaatcgttc tggtacatgg tcaaaagttt tgcgtgcttc
    22801 attactaggt gcaatttctt tcaaccacag atctaactgc gctttttctt ttgaaattcc
    22861 tcgtggccag aacctatcca cgagtattct atagccatcc tctttttcag catcaaggta
    22921 aacacgtttt aatttgatca tagttgtttc cttctttctg ccttttcgct tagcttatat
    22981 atgtagataa tagcacaaaa taaaaaaaat gaacggcaca actatccatt gcgccgttca
    23041 tgtgatcttg gtcaaaatct aatgattgat tcggttttta aagctgtcat acaatcatct
    23101 gttttatact tttttacttt tgatttttcc agaccatccg tcatatccac ccttaagtaa
    23161 ataaatctct tgataacctg ctttacgtaa tttatttgct gtacggatgc tcaagctttt
    23221 cttttgatcg taaatataga caggctggtc ttttcgaata gatcctaatg aatctttcaa
    23281 aacagtgtaa gggatgtttc ttgcacctaa aatatgccca gcatcaaaca catctttttc
    23341 gcgaacatcg attacttgtg cttttctcat tccttctcga aattcttctt ctgtcaaagt
    23401 cgttgctgaa cgcttggcca ttactcgtag atagacttcc caaaagatga ttgcaagcac
    23461 aatcaatagc aaaatgatat taattaccca taaaaccatg taatgcctcc gtttttttaa
    23521 gcaaatttca aggctaatga ggcagcacca atgacaccag cttcattacc aagctctgct
    23581 aatttgattt ttgttgattc tcttacttgc gggaaagtaa attctttaaa atatttctct
    23641 acacggctac gtaaaaattc tcctgctgcc gatactcctc cacctaacac aatacttgac
    23701 ggattcaatg tattccccaa gttggcgcaa gctaaaccta agtagaagca tacgcgatca
    23761 acaaccatca atgcaaacgg atcgtcttct tgagccattt caaagatatc tttacttgtt
    23821 acttcttcac cgttgtctaa cattgctttc agttttgaat ctcctgcgta ttcttctgcc
    23881 aagtaacgac ctagacgtac cacaccagta gcgctagaca cagtttctaa acatccgcgt
    23941 ttaccgcaag tacattcgaa cccttcagga tccacagtga tatggccgat ctctccgcct
    24001 gctccgctca cgccgtgaat caaatttccg ccagcaacga taccaccgcc aacacctgtt
    24061 cctagagtaa cgaatacaac gtctggatcg ttttctccag cacctttcca tcgttcacct
    24121 aaagcagcaa cattcgcatc attatcgata gcgaatttga ttcctgttcc tgcctcgatc
    24181 agttttttca caaattgaac ttctgtccag tttaagttat acgcaccaat aaccgttcca
    24241 gcttcgctgt ccacgcttcc tggggtaccc atgccgattc cgataaagtc ttcggctttc
    24301 atattatata aattcaaacg atggttgatt gattcaatga tttcaggaac gatatgctta
    24361 ccatcatcca aaatattggt atcgatgctc catttttgtt gaatctctcc ttctggtgtt
    24421 aaaattgcaa attttgctgt tgttccacct aaatcaatcc caataatctt cttgtccatt
    24481 acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc tctttttaat atgcgccacg
    24541 ctgtcatgta tgtatcatga tcaatcaaac ggctatcata cagattacgc aattcgatag
    24601 tcattaattc aatgtcatag atgcgtgcac ccacatagat aaagatgcca aattttttga
    24661 acagctgctg tacatcatat aagctctcca tctttaagca cttccttcat attatacaag
    24721 taaaccatat ttttttaaac ccagtaaaat acatattaca aaaagaaata caaaaactag
    24781 tgtagaaaga atcctttggt gtatcgaata gcttgtctgt tgtttcggta gacccaaaac
    24841 attccctaac actaatccgc caattagccc accgacatgt ccccaaacat ctacagaggt
    24901 atccagaaga ccaaaaacaa atgtcaacac aataaataaa agaaaatgtc tgactagctg
    24961 ctggatcgct gggttatgtt taaaatgaaa ccctaagata aacaacgcac cgaacaaacc
    25021 gaatatcgaa gtactcgcac caccagacag aacattcgct tcgttaaaag caaagcttgc
    25081 tgtatttccc attatcccgc tgaacatata aatcaagaaa aaacgccaat ggccatagat
    25141 tgcctcgatt tgctgcccca taaaatacag tatcagtgag ttcattacga aatgcatcag
    25201 tccaaaatgg atgaagattg gtgtcactaa acgccaccat tcattgaagt gaacaagata
    25261 aggaccatac attccggcat aataagggat ctcaagtcgc ggaaataatt ccatcatgag
    25321 gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat aacggtcgtc ttaaccatgc
    25381 acgcaatttt atttgctgtt gataattcat gaaaacctcc catttcattt tagtcaataa
    25441 acaattgttg gatcttctga tcaaagctct ccggttgcca ttgttcattc aattgttcac
    25501 gaaaaacaag gctacatgtt tttttatgaa aattatttaa ataccggtcg taataaccac
    25561 cgccaaaacc gattcgataa ccggctctat tgtagataat acctggtaca atgaggagat
    25621 cgatcatctc cggcaaatag agcttctctg attgtggctc aagaacacca aaactcgaac
    25681 gtacaagaac ggactgttca tccatttcat aaaacgccat ctgacgtttc ggcaaagttt
    25741 taggtacaac tattttcttt tgttcttttt ctgctcgttg gataaccggc tgtgtatcca
    25801 gttcaaattc attcgataaa gtcgtcccta tcacttttgc tgacttccag tactcgctgg
    25861 cgaatagctg tttatagatt cgctgttctt tttccttttt cttgccactt ttggccagat
    25921 ttttcagctt ttcgattcct ttccggcgta atcttacttt caattggtcc aaaaactcac
    25981 ccttccttct ctcataaaat ggtataggaa aaaaagaaaa atagcaatcg aactttcaaa
    26041 taaatgcata aatagaataa aaagaagacc tagaaatgat tggccttcct tttttaataa
    26101 atttcaactg ttcagcgcgt gtttttgtct gataaaaagt gcatacagaa aatatggcgc
    26161 accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca cttggtagaa tcactctagc
    26221 aaccatatct gcactaagca gaaaaatgga accgaacaaa ccgctcagaa caaaggtcca
    26281 gttattttct ttacgtacga ctagcttcgc agcatgagga gctagtagtc ctacaaaaga
    26341 taaactacct gtcaccgcta cactaccgct tgccaaagca acagctaaca acaaaaggat
    26401 tttttgactt ttagccacgt taaggcctaa tccttgcgcg cgttcttgac caaatgatag
    26461 caatccaatc ctctgtgcat aaagtcctcc tagcggaatt aagataaccg cccatggaag
    26521 caatgcccaa acataaggcc agctcgcacc ccagattgat cccgctaacc atgcattcac
    26581 aaatccataa ttttccttag ataattggat cgtgcatagc aatgttaatg cagaaaggcc
    26641 ggcattaacg gcaataccgg acaataagat tcgattcata cccaaaaagc catttcgatc
    26701 ataggccgca agatagacac agcaagcagc taaaacacca cctagaaagg ctacaactag
    26761 caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa taaagcatca ctaaaaatcc
    26821 tgctccggca ttaatgccca atatactaga atctgccaat tcatttcttg ttactccttg
    26881 caagatataa ccagaaacgc caagacaagc tcctgctaat aaactgatca gtaatcgagg
    26941 aactcgaaat tctctaacaa tcagcgccaa agaagatgat tctttcccta tgaacgtggc
    27001 aaaaagctga tccaggtaaa taaaaggcgt accaatcatt aaactaccta tagacaggat
    27061 gacaagtaca aaaagcaaga aatagaacgt ccaatatatt tttttcaccg ttgttccctc
    27121 cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa tgactaatcc aaaaggtgtt
    27181 tcaaaagggg gattgatcat cctcgctgta aaatccgcca acataaccaa tccaccgcca
    27241 actaaaaaag agacaggtag aacataacga tagtccgttc caaccattcc gcgaacaact
    27301 ggcggaacca acagcccaat aaatgaaaca ggtccgacta acgaaacggc aatggctgct
    27361 aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt taggatgact tccaagagct
    27421 aatgcatgtg tttcactcaa atgaatcaaa gatattgctt ttcctaatga aaaagaaacc
    27481 aaaacagcaa gtatatataa aggaaacaat actgctaatt gtttccatgt gatattggca
    27541 gctccgccta caaaccaaaa agcaagatcc tgctgcagat taaagaattg ggtaagtgct
    27601 tggctgatag cagtaaaaaa agaactaata gctacgccag ctaaaatcag ctgtaaagga
    27661 ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga gcaaagttac agcaaaagaa
    27721 cctgccaatg acattagaaa cacagaaaaa tcagaagctt taggccaaaa agcaaaacta
    27781 agggccattg ccaacgaagc acccgcgttg attcctagta agcctgaatc tgctaaagga
    27841 tttcttgtca ctccttgcat caatgcaccg ctcaatgcaa aacaactccc tacaagaaat
    27901 gctcccgcca cacgaggcaa acgaatattt cgtatgattt ggtgactttg ttttgatcta
    27961 tcaaaatcaa acaacgcctg ccaaaaatct gcttttccca cagtcgctgc gccatttgaa
    28021 agagcaacaa aaaaaataat caaaagaaca aaacagacca aaagcaggag tctatttttc
    28081 ttcatagtcc tcataattga tcctcctgct cttttggcaa caaatcatag gttagaaaaa
    28141 ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc aatctcaaat atttttttca
    28201 tacactcttt tgtgatcact tcttcaggtg taccttctgt gatcaactta ccgtttttca
    28261 ttgctaataa ataatctgaa aaacgtgaag catgattaat gtcatgaatc gacatcaaga
    28321 tcgttgtttg tttcatttga ttgatttcag tcaataactg taggatctct aattggtgtg
    28381 ccggatctaa aaatgttgtc ggttcatcca agatcaagat atctgtttcc tgagcaagcg
    28441 ccatagcgat ccagacacgc tgggtttgtc caccagacag cgttgcgata tcccgcatac
    28501 gaaatccagc tagacctgta gcatccaacg cccactcgat tgcttcatga tcttgcgtgt
    28561 ccatgccact gaacagtttt tgataagggt atcgtccgta agatactact tcttctactg
    28621 tcatacctgt agggtgttca ctagtttgtg ctaataatgt aatttttttt gcaagttctt
    28681 ttgaactgta tgtagagata tcagagttat tgacaatgat ctttcctgat ttcacaggta
    28741 gtatacgggt aaaacttttc aataaagtag attttccact tccatttggc ccaatcaaac
    28801 tagtgatttt cccttcagga atcgtaacag acatgttttc aatgattttt ttgttttcgt
    28861 agccggctgc aatgtcagct gctcttaatc tcgtcatttc acccatcctt tcgctgaaaa
    28921 tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc gctactttca tatctatagc
    28981 caaatgaagc ggttgacagc gttcgaaata tcaggaataa taaccaatga gaaagattct
    29041 caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa gtattcacat tatctagcat
    29101 attgctgctg gctggagtac tgagcgcttg ctccccctct tcagataaag aaacagctac
    29161 aactgccaat tcgacgactt catccaatag tactcataca gtcacagata cacttggaca
    29221 taaagtagag attccaaacg cgcccaaacg gatcatcggc agttacttag aagactatct
    29281 gattgcacta ggggaaaaac ctatcgccca gtggactgtt ggcagtggtt ccattcaaga
    29341 ttatctgcaa gatgatttaa aggatgtccc aacgatttct tatgaccttc cttatgagaa
    29401 agtgttaagc tttgagcctg atttgctgtt gatttcttct tccgctactg tcgaaggcgg
    29461 gaaatacgaa caatatagca aaatcgcgcc aacttatgtt gtaaaaaacg gaacagatgt
    29521 cacatgggaa gaacaactaa aagatgtcgg caaagctttg gcaaaagaaa aagaggcaga
    29581 aaaaatcatt tccgattatc aaaaatccat caagaaaact cgagaagaac tagcagataa
    29641 aatcgagaac aagacagctg cagtgttgtg ggtgaccaac aactcagcct ttatggtcag
    29701 caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg gaatttggtg ttcctgatct
    29761 agtggaagag gtctccaaaa aagccacttc tgactggtct gctgtttcct ctgaaaagct
    29821 agctgaatta gatgctgact acatcatcct tgtgaacagt gacaaagatg ctgccatgtt
    29881 caatgaacca agctggaaga atcttaaagc agtgacagac aatcatgtac tgcaattcgg
    29941 acctgaatct agttggctat ataacggacc tattgccagt caggaaatgg tcaatgatat
    30001 caaagaaaaa ctaaattgat tttaactaaa aaaagcatct tcacgattag tcgtgagatg
    30061 ctttttttct tattaacaaa ctagacgcat agattttatt cttcttttga agcaacccta
    30121 ttttttactc tttcccattt ttgaaaacga tgggcatata gattttcttg atccgttatc
    30181 ccctcatact cctctataag ggcgaagtct gaaaaatcaa tattcccaat atacgtatcg
    30241 ccttcaaacg tgctatcgat caatgttcgc cagatccgac cagtgtaagg aagaagttct
    30301 tgaaaaatcc gactaccacc cgaaacatag atatcttctg agatcgtttg actatacgac
    30361 aaaagttcat cgatcgaatg cattacttcc gcattttctt tcgtgcgaaa atttggttgc
    30421 gttgtcaaca cgatgatatg gcgataagga agtgaaagat cccccattcc ttcataggtc
    30481 tttcttccca tcactaaaat tcgatccatt gtatgttcgc gaaagaaacg catatcattt
    30541 ggcaatcgcc atggtaacaa accatttttg ccgatcagac catttttatc ttgtgcccac
    30601 atggaaataa acatattatc cgctccttat gattttagat gttttgagat aaaaaaagtc
    30661 tcaattcttt gaccagcaag gaattgagaa ctttaaaaac attattttgt ttcacggtgc
    30721 aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt ccaaacgatc aggattgtta
    30781 cgtttgtttt tgcttgttag atagttacgt tctttacaag aagtacactc taaagtgatg
    30841 tttacgcgca tgtttttccc tcccattttt acaagaatcc tctaagatga ctctcagact
    30901 tgattatctt accatgattt tttctagatt gctagtgttt ttcacgattt tgttaagtaa
    30961 aactacttga cgataaatac aacggtttta cttctcaaac aaaatctttt tctttttgta
    31021 taaataccct tacacttgtt tatatagatt atgtttttta ataaaggggg atactaatat
    31081 gccatggaac atgaaggact ttcctgcatc aatgaaaaac ttagataaat tgacaagaaa
    31141 aaaagcaatc gacatcgcaa atgcattatt agatgaaggg taccctgata accgtgcgat
    31201 cccaattgcc attgatcaag caaaaaaatg gtatgaagat gctagtgaat cagaaaaacg
    31261 aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa cacgatacga atcctcgtgc
    31321 tggaaaactg cttgattcag atgtgatcgt agaatatgag gaagaacaat ggatcgtgaa
    31381 atctaaaggg gcaaaaaagg caagcaacca ttttgataca aaaaaagaag caatcgaaaa
    31441 aggaaagcaa gtagcacaaa acaaagaatc aactttgttc atttataaaa aagatgggac
    31501 aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg ttgtgacgaa aatcgtgtca
    31561 caaccccctt atgttcgaaa caatggtgtt cctctgccaa ctccgaacca taagtcataa
    31621 tatctataaa acgggaagag ctgttttttg aaattgtttc ttatcactca gtgttaaacg
    31681 gtaggagaaa acctcttttt tattgatatt tgattttcat caaacagtca catagtcgat
    31741 tgcttctgct tgcggaatcg tttcatctac aaagtagact ttataccatg tcaagaaact
    31801 gaagattgtc cagatttttc tttgctcttc tttacgtcct tcgtgatggt catctagcaa
    31861 cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa tctgcttcga ataatgcttt
    31921 gacttgttcg tagccgtgtt cttctttcaa ccaagctttg attggcacag ggaatcctaa
    31981 tttcacacga ttcgaccatt cttccggaag atgtttgttc gcagcttgtc tgaaaatatc
    32041 ttttgtgttg ttttgattca ataaaaactt acttggtatt tgttgagcca atttcatgac
    32101 ttcgatatcc aataaaggta ctcggacttc caaagaactt gccatagaaa gtttatctgc
    32161 ctttaacaag atatcttttg gcatccattg atgaagatcg acatattgca tcttattgac
    32221 ttcatctttg attccttctg ttttctcgta atgaacagtc atgatttcat ccacagatgg
    32281 tgctgtttga tattttggtg ttaacacttc tgatgcttct gattcttcga acactcgagc
    32341 atgaccgata aaatattctt ttgcaggagc aagtgattca tataaatgga tccgtccatg
    32401 gaaatttttc atttttccga ttttacgtcc taaattatag cgtgttcctt ttggtaattt
    32461 tttcaatcct tgagcaatca cacggatgaa ttttgaattc gtatggaatc catacgtttg
    32521 gtatcccgca aacagttcat ccgccccttc tccagattgg actacccgca cactttgtga
    32581 agcaagattt gctaaaaagt acaaaggtac gcaagaagga tttgaatcag gttcatctaa
    32641 gtgatactgg atcaaaggga atgctttgaa cgacatgtct ccgtcgatca ctgctgctgt
    32701 attatctaag tccaatagtt cggttagttt tctggcttca atcgcttcat tataggtttt
    32761 atcatcaaac ccgattgaga aagaatgatc tggtttcaat acagaggtta cataactgga
    32821 atcaaccccg ctggataaga aagaaccaac ttctacatca ctgaccgtat gtgcttcaat
    32881 agaagcttga acggtttcgt cgattttttc gatccattct tgtcgggaat gcgtttcttt
    32941 tgtttcaaaa tctgcatccc aatattgctg aatgtctagt tttccatctt tatacgtata
    33001 ataatggcct tcaggcaaac gatagacatc tttgaagaat gtttcgccgt ttaatggaga
    33061 atattggaaa gtcatataag gtttcaacgc ttctttgttc aattctttgt taaaatcagg
    33121 atgaggcaga aaacttttga tttctgaacc aaacataaat gttccgttca tttctgcata
    33181 ataatatggt ttgatcccaa agtggtctct tgcaccgaat aactcattct tttcattatc
    33241 ccaaatagca aaagcaaaca ttccgcggat cttttgcaat aattctgttc cccattcttc
    33301 gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga aaaacgtggc cagctgcaat
    33361 caattcttct ctcaaaggct ggtaattata gatttcgcca ttaaaaataa tgatctttgt
    33421 ccgatcttca ttgtaaatag gctgagtacc gccctctaag tcaataatac ttaatcttct
    33481 gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa ctattaggtc cacggtgaac
    33541 gattcgatcc atcattgtat taataattgt ttttttgttg tccttgtcgt taacaaaacc
    33601 tacaataccg cacatttaac tttcacatcc taatataaaa ttactgttta tttcattcat
    33661 atgcatctta tcatagaata aatagcggac gaatattttc tctcttacaa tttcgtcacg
    33721 tgaaaacaaa agatgaaagt ttctgttttt acagaaaata aagtcccaat atctaattta
    33781 ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt taaaaaaata ttagtggatg
    33841 ctatttccaa aaaaaacagc aaaaaaagac acgacctaaa gatcgtgtct aattatgact
    33901 gtatcagaaa atatgatacc aaacggatct attcattaat taattgacat agtttatctg
    33961 ttgctctttt atgactggat aactgaccaa ttcatgacga tccaagtctt ctttatttat
    34021 atttacagca gtaatttgac tgtcttcata cgtacgataa taataaatcc ctttatcaac
    34081 gttacagcaa gaagaataaa tcgtatattc gtattttcct tctccagtat cgcatactcc
    34141 tttttgttgc tcgactgagc ctagtatatg aaaaaattga ctgatacttt cagattctga
    34201 atcacctgat gcagcattca ttttagtaaa tgtggctttt acaaatcgag aaacagaaga
    34261 gagatctcca ggtaagccca atcctcccat cccacgacta taaacattca agctgatctg
    34321 gtttgaaaaa tgattttctg gtgttccatt tgatagcgag cgataattgt tcagattaaa
    34381 taattgataa tcaaatggcg gattatttgt caacacaccg acaggattat catataaatg
    34441 aagtccatct ttcatacttt caattactat cgatttttct ttatcagcta ataaccagtg
    34501 taaaggtgac aagggtagtt tttcactaaa atcgatattg actagattga tagtagccaa
    34561 taattcttta gcttcgtcta tggttgagca ttgccctagt atccatggaa taaactcaaa
    34621 tggtgcaacg ttatcttttc cttcttgtaa ttctttataa tcagcattgc caggaaaatt
    34681 cagtcctgcc atactcaacc ctttttcatt tgtagcctca taatagagtg gatagttgga
    34741 taccccagct gctattccta tcattgcata atgtttatct aaatttttta cctttctaaa
    34801 atcgaaacga taatttcttg gagtaatagt gactacttca ttataagaca tctcataatc
    34861 aaaattcctt ccaaaataat ggtctttcgt tgcataagtg attgcggtac acatcgtttt
    34921 ccctcctaaa acaaatcgtt tttacacttt tatcttacat ctagtatttt tttttatcaa
    34981 aaaaaaggcc aaagaagatt gatttactag catttataaa aaatagtact cagggggaat
    35041 caaaaaatat tgtaggttaa aagtacaaca ataaggggct gtgacaaaag tcttgtcaca
    35101 gccccttatt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    35161 aacgaaaaaa taggaaaagc tatttttgtt tggccgttct tattacttga tgcaggacag
    35221 cttcttcaca acctctaatc gttgatataa ggaaggtaca ggatttgaac ctgcgcgccg
    35281 gtattagccg gttcgccgga tttcgagtcc ggtgcattac cactctgcca accttccgta
    35341 agtactattt ttaaactttt ttcacgaaat gtcaagccta tttctaatag ataaataaca
    35401 gaaatcgctt tttttagcaa aaaagtccat tgacagtttg cagccaacgg acttaccgat
    35461 caaattcata ctgatagaac actatctgat tatttcagga ttatctaatg ctaaaatcga
    35521 gtcaattgtc cgtttgatca gtgcaatgat ttgttcttct gttggatgca tgatgtttga
    35581 tgacatcaat gacgctaagc cagtcactgc tacccatgta ccagtatgca gagcatcgac
    35641 atattcatcc gataattgat tgtatctttc atcttcttta actatttcac ggaaataatt
    35701 gtatgaaaaa tcatgcattt tctttccgtc cccatgttca tcaatgtaca atgccatgta
    35761 aagactatga tgcttttttg caaagtagat atagttcaaa ccgagatcaa tgattttatc
    35821 accagtacgt tcaataggga aaacatcttc ttttagttgt tcaaagatag aatccagcaa
    35881 cgtatttttt aaatcttcca tgtttttgaa ttccagataa atcggctgtg tcgaaatccc
    35941 catcttttta gcaatatttc tcgcagtaaa tcctttaaac ccttcctttg caacaacatc
    36001 ataagcagct tttagaattt gatccttggt atagactttt cttctcatta aaactccccc
    36061 acttctttac tgcagtatat gttgttttat tgtaccctat ttttagcatt ttttgcaaac
    36121 attttactca aaacgttata ttttaaattt ttttcagtct tctttaactt ttttttgcaa
    36181 aattaaaaaa atttggttaa attcaatggg ttcccttaca tttttagttt tgacctaaat
    36241 gatagttgtc caaatctttc atttcaagtt atagttaaag aaaatgaaaa agaaggtgtg
    36301 taatggaaaa cgaaactgac gttatttata ttcatcctca aaaacgaatc gtttcacaga
    36361 aaagaaagta tttttatctt ggttttacag gtgttttttt tctttttatt ggcttgcttt
    36421 ctaatactcc tactgataat tggagtggat tgctaactat tcttacctcg ccaagcaatc
    36481 ttttgacaga ttattttgct ttaggtgttt tcggaagcgc attcataaat gtagggattt
    36541 tgaccttact ttctgtgctt ttagcttatc ggcacaaggt gattttaaac ggtcccttat
    36601 ttgcttcgat tctaacagtt accggtttct ctttttttgg caaaaacctt tataattcta
    36661 tctcaatcat tttaggtgtg tatctatacg ctgtatttgt caataaacct ttttctcagt
    36721 acatcatgat cggactgttt ggtagcgtat tatcaccggt cgttagttat atcacttttg
    36781 gtatgcgttt tcctttactg ataggtattt tattggggaa tttagctggc atagcaattg
    36841 gtatacttct tccgccctta gctgctcaaa ctttagtttt ccatcgtgga ttcacacttt
    36901 ataatattgg attcacctct gggttgattg cgatgacttt tactgctgtt ttgaggttat
    36961 tcagctattc aatcgtcgaa aatacattgg ttttcaatga atatcacttt cctctcattt
    37021 ggattatttt tggctttttt tcgctgacag tcggaattgg tttctattat aattcctttc
    37081 ggctttcggg gattcgagaa attttcgatt catctggaaa attgacaact gatttcattg
    37141 ctaattctgg tattggtgct actttgatca acatgggctt agttggtctg atgctgtctt
    37201 cttatgtttt gctggttggc ggtcagttaa atggacctgt catcggtgct attttatctg
    37261 cggtgggttt cagtgctttt ggttgtcatt taaaaaacag ttttccgata ctagtcggaa
    37321 tatttattgc ttctttgttt ggaacttttc atgagatcac cagtactggc atgcttgttg
    37381 cagctgtgtt tgggacaggt cttgcgccca taagcggttt ttacggcagt ttttacggtg
    37441 taatagctgg tgtgctacat attgctcttg tccataacgt cagcactttg cacggaggac
    37501 taaatctata taacagtggt ttttcaactg gtttcgttgc aggtatcctt gtaccgatac
    37561 tagataactt taccgctgtc agaaaggaaa aaaagacact tggaaaaaga attatcaaaa
    37621 agaatcatcg atgagcttct tacttattta tattcccatg aaataactga aatacgtatt
    37681 ggagtagaat ttgctgagga agggcttttc atagagatac agggtacggc agaaactcag
    37741 ccagaagatt tagatgtact tgctgatttg ctgaacaatc ctcgggaccc tagtatggaa
    37801 atctactatc aagaactatt aggtaatccc cgacatgatc gggaagatta tcatctgtta
    37861 ggtagtttga ttgatgacgt ggaaatcatg tatgcccagc cgattatcaa tattaagatc
    37921 tttcgacgtg cttctcattc ctattattaa ccctattttc tatttataaa agactgaatc
    37981 aaaaaagcag ctcctgaaag ttcgattaaa atctactttc aggagctgtt caagcttgac
    38041 attattgatt ttccaatcct tcatacactt catctgttac aggttctaaa aactctgaat
    38101 taccagcagt gatcgccaaa tgttcaaacc aactgtcttt tgtcgcacca tgccaatgtt
    38161 taatcccttt gtgcgttacg acgacatctc cagccattaa tcgtcttgct ggctttcctt
    38221 cttcctgata ccagccttct cctccggtca caagtaagat ttgatagccg tctgtatgga
    38281 catgccagtg attgcgacaa cctggttcaa aagttacatt acctacacca acagaaactt
    38341 cgggatcatt cactaacata ttcagatagc tttgcccggt aaatacttga gcgtaagctt
    38401 cgtttttttc acctgctgga aaaatcacgc cattgtgtat ttcttcgtgt tttgtcatca
    38461 tttacgcctc tttcttatct tcgctatttt cttctaaata gatttctttt gctaaactaa
    38521 aagctgacca agcttttggc cattctacat aaaaagctaa atgggtaatg atttctgcta
    38581 tttcttcact agtaatgcca ttttgcttgc caatcttcag atgagctggc aattgttcaa
    38641 aatttcctcc tgaaattaat gcagatatgg tgatgatact acgctgatga gcagataatt
    38701 cttctctgga ccatacttgt ccaaataaaa catcatcatt taattctgca aattttggtg
    38761 caaaatctcc taattgatct cgtcctgcgg tttgtttttc tgccatttct cttattctcc
    38821 cttcacgact gttaggtgat tcaactatgg ttctaaggca actttatttt ctattgtttc
    38881 ttcgcatttc attttaaatg tagtctttca gcctgtccaa tgcttagttt attcagtcga
    38941 tatgcttttt aagcatagtt gctaacgctt tttttatttt aaaaaaaaca agttattaaa
    39001 taagtcttta gttggatgtt tttttgagat caatctttta tacttatgat gaacttacaa
    39061 aattgtttgt tttctgtcat atcaaaaaat ggcgcagtta gttgcttttc tagtaaagta
    39121 tagttaacaa agaacctcta aggagttgat gaataatgaa tattgctgcc tctcgaaaac
    39181 tgataaattt catatccatt atcggattag gcctatcaat cgcgctgacg atttatttta
    39241 ttaatttagg tgttttcaaa gatttgaatg cgttaagagg attagtaggt gattccatca
    39301 ttttaggacc gattattttt gtcttcatcc aaatcctgca agtagttatt ccaatcattc
    39361 ctggagggat cagtactgca gctggtgtcc tgatatttgg cccttatgct ggatttatct
    39421 ataactatat tggaatctgc attggttcga ttatcatttt tctgctaggg cgtcgttatg
    39481 gaaagccatt catactttcc atgattagcg ataagacata taacaaatat attggctggc
    39541 tggataatca aaaccgtttt gagaagctgt ttgctctagc tatttttctt ccagttgctc
    39601 ctgacgatgc gttatgtttg atggctggat tgacaaacat gtcagtgaaa cgatatacac
    39661 taatcatttt gattgctaaa ccactgtcga ttttcttata tagtatggcc ttgatttatg
    39721 gaggtcaata tctaagtggt cttcttggat aatatttatt ttgtaaaatt agtcattgat
    39781 tgacaatgtt aaatctggga aagggtctgc aaaacacact gcaccctttc ctttttcatt
    39841 agatttgcgc tacaatgtga agagcaggtg ataggatgaa ttatttgaaa cgtagcttag
    39901 gtaatgcttg ggacttttta aaaggaacac aagcttattt ccgtgatgtc ttgctgatgc
    39961 atggctttat tttatttatt tgtttaccct tattgagcag tacgacccgt tttattttac
    40021 accgaggggc aattgactat ttgtcttatg acaatattcc ttcaattttt tctcaacatc
    40081 ctggggttct actctcgtta atcgtagttt tgatgacgat tttactgtta gtttatttcg
    40141 aatttacttt tcttttgatg agtgtttttt ttattaaaaa gaaagaacca atctctttga
    40201 agcaactctt acacttgacg atacttcagc tgaagaaagt gagacccatt acttttttgt
    40261 ttttccttgc ttatttttta ttgattctac cgatcagcgg attaagtttt aattctgatc
    40321 tactgtcaaa aataaaaatc cccgctttca ttatggattt tatttttgct aatcgttgga
    40381 tcatcgtcag ttccttttta ctcgtttata ttttccttgg ctatattggg attcgactga
    40441 tttttgcttt accggaaatg attctacgag atcgttcttt tcgagcagcc attcgggaaa
    40501 gctggtcatt gaccaaatcg cgtcttttgg ccatcacggg ccaatttatt gtgattggcg
    40561 gtacgatttt actcctttct tctttaggat atatcgttgt tattttggca caaagtatgg
    40621 ttgaacaatt tttcccagac tatgcattga tcagcgctgt gtttgctatg acgcttttac
    40681 aagggatttt attattcaat atcgtgatgt ctaccgtagg gattttttat atcattgttg
    40741 atttcatgga tgacgaaggc ttcttgcctg agatccctaa gtggtttatc ccacaagccc
    40801 ccaacttgcg tttctctgcg ctgaaaaaca ctggtttgac tctttttgca gtttttttcg
    40861 gtattggcgt ctgtctttac aatatggatt atctgacttc agctgttcag acaaaaccag
    40921 tgactgtctc tcacagaggt gtgagtaccc aaaatggggc gcaaaatact ttagaagcac
    40981 ttgaaaaaac aagcagagac tatcatcctg attatgtaga gatggatgtt caggaaacaa
    41041 aagatggtca tttcgtagtg atgcatgatg caaatcttcg tcacttaaca ggggtaaatg
    41101 gaacaccaca ggatcttaca ttgaaagaat tgacaaatct tacagttact gaaaacggag
    41161 tgcaagcccc tgtttgttct tttgattctt atttggaaaa agcacataag ctaaaccaaa
    41221 aattacttat tgagatcaag acctctaaaa aagattcgga tgaccttgtc gaacgatttg
    41281 tccataaata ccgcaaagtt atcttagaga accatcatat tttgcaaagt ttgacgtacc
    41341 aaaccgtttc agatttaaaa gaagcagaac caaactttta tgtgggctac atcttacctt
    41401 tcaatattgt tgggccccct gtgacgcctg ctgattttct gacaatggaa tatagtacga
    41461 tcaaccgtaa cttcattaat tctgcccacc aagacggaaa agaagtatat gtatggacaa
    41521 tcaacgattc agatgccatg agtcgcatga tgttttatgg tgtggatggt atcataaccg
    41581 atgagatggg actactaaat gaaagtgtga aacaaacaga tgaagaaatc acttattcag
    41641 ataaactgct aaattttgtt ttgggagtcg gctaatcaaa aatcgttaat gaaaaaagtc
    41701 tttatacaac aaaaaacgca taacctgcca ccgcaggtat atgcgttttt tgtttgacgc
    41761 tcataatgtg tgcttaactt tctcttgatt ccttcatcgc acgaacaata gctaaaatag
    41821 caggaattgc agagatcact gcaagaataa ttcccatttt ccctgaactt tttctttttg
    41881 gcatatcttc tcgctccctt ctgtctttat ttatattata agggagttct tgcgaattct
    41941 caaaaatact gtttaagaaa ccaattcagt aggtgcgcta atatcacaga attttacgag
    42001 catgcgatac ttgctttcat tgataagttg atagtctcgt tggtcataac tttcaacata
    42061 cacgacagcc gtgttgatat atgtacgttc aatacagccg attagttcat gagatactcc
    42121 gatcggtttg catatataag atcttcctac ttctgctttt accatttcat tccctctttt
    42181 ctttattttt attttgtttt ttctttattt tataataaca aattaccttg tatttgaaaa
    42241 cgtttttatt cattttttta ttttttattt tgagtatctt tctgcttttt agataactat
    42301 tttttaacac gatcttagac gttgtgacaa aaacgtttgt ttatattcct atgttgttcg
    42361 tgcgtttttt tctttatatt gctaagttat ttataaagag gtgagcatat gatttatatt
    42421 caagcaatag tatttgtaac cttgatgatt gggctactta tcggattaaa agaaaatagc
    42481 taaaccattt aagaaaaaat aaataaggaa aatgataaat gctttttaat tgcatcaaaa
    42541 aacgtacgat gctaaacttg gtataagttc gaaagtttaa aggaaacttt caagaactaa
    42601 attacattac aaaggagatg aattttatga cgtttacaaa aacaaatatg caataccgcg
    42661 tagcttttga tacaacttct aaccgtttta tggcgattga tgcacaaaac gaaaataacg
    42721 ttgcgtttgg tattacaatc gaacaagcag tccgagcatt aaacgaatct agataaggag
    42781 tgaaccattc caaatggcga ctgatgagat ctcggaaaat caataaaaga cgtttgtaac
    42841 ggtaatccgc gacaaacgtc tttttgtatt cattacatat atttggaaat tgtctctctt
    42901 tatctctaga cctaaacaaa aacacaagtt ctaatatcaa ccatctcgtt ttttatgaca
    42961 attttgttag ctgtttcgat gataatattc cgttattatc tgtataatat ttaatataat
    43021 aaaaataaaa aaggagatct ttcatgaaaa aaattttagg gttgatcgtt atcatattga
    43081 ttgcttttat aggatggaaa ggttgggact actatcagtc tacttatgta ggtaaagatt
    43141 attatgcggt agtcaaagct ccaatgcctg cagaaactga tatcaaagca gataatgggg
    43201 aagtaattgg aagaggtttt aaatacaacg tagatgctta tgctgaaaac ggtgaaaaaa
    43261 gacagttaga tttcgatgtc attacttctg gtgactctgc caatggttct gcttacaccg
    43321 aaggaacgat cttgcagttg aaagcaagcg aaaagcggat catagagaaa aaagtaatta
    43381 ccagcgataa ggtaccctca tctgtaaaag aaaaattagg cctatcttaa gctaagaata
    43441 tatcgcaaaa aagcactcga agtgagtgct tttttgctta ctgattctag aaaacaaaaa
    43501 aacagaatta accttcgaag tcagcctcac tgttcttaaa gtcctaaaac aaactaaaga
    43561 gcaaggcaat caatcgattt ctatacaaac aagggactgt gacaaacgtc ttgtcacagc
    43621 cccttattct gaataaacga tgcagtatgt ttgagtacct aaatgggtac cagttttcat
    43681 tttaaagcga acatgtttct atcactgatt atttaaatct agcgaaaaac ccttatctta
    43741 caggcgttcg ttcaattctt tagcaagatc ttcaaagccc ggtttgccta gaagtgcgaa
    43801 catgtttttc ttgtaagctt ctacacctgg ttggtcgaat gggttgattc cattcaagta
    43861 acctgaaata cctacagcaa tttcaaagaa gtacattgta taacccaatg tataagcatc
    43921 catttctgga attttgacta gcaagttagg cacgtcaccg tctgtatgag caagcaatgt
    43981 tccttcaaat gcttttgtat ttacgaagtc cacttctttt ccttgcaagt agcctagacc
    44041 gtctaaatct tctgcttgtt caggaattgt gattgattta cgaggttttt caaccttcac
    44101 aactgtttca aaaatattgc gacgtccttc ttggatgaat tgtcctaaag agtgcaaatc
    44161 agttgagaag tttgcgcttg aaggatagat gcctttttgg tcttttcctt cagattcacc
    44221 atacaattgt ttccaccatt ctgagaagta ttgcattcct ggttcatagt tgatcaacaa
    44281 ctcagttact ttgcctttac ggtaaagaat gttgcgcatt gctgcgtatt ggtaagcttc
    44341 gttttcttct agtttgtcgc ttgagtatgc tttgcttgca tcagcggcac cttgcatcaa
    44401 tgcatcgata tctgcaccac taacagcgat tggtaacaaa ccaacagcag ttaatacaga
    44461 gaaacgtccg ccgatgtcat caggaatcac gaaagtttcc caaccttctg catctgcttc
    44521 tactttaact gcaccttttg ctttatctgt tgtagcgtag atacgtttgt ttgcttcttc
    44581 ttgtccatat ttcttaacaa gaagctcttt gaatacacgg aacgcaatcg ctggttcagt
    44641 agttgttcca gattttgaaa taacgtttac tgaaaagtcg cgatcaccga ttacttcgat
    44701 caagtcagca atatatgttg agctgattga atttccggca aagaagattt gtggtgcttt
    44761 tcttgtgttg ctatctaata aattgaagaa tgtgtgattc aagaagtcga tcgctgcacg
    44821 agcgcctaaa taagaaccac cgataccgat aacaactaat acttcagaat cagattggat
    44881 ttttttagct gcttctttga tacgtgcaaa ttcttctttg tcatagttcg ttggtaaatc
    44941 gatccagccg cggaagtcgc ttcctgcgcc agtaccttcc cgtaattcgt tgtgggctgc
    45001 tgttacttgg ctttgcatgt accctaactc ttgttcgttg acaaacggtg ctacttttga
    45061 atagtcaaaa tgaatgtgtg ccatttgttc tcctccttat aggtttactc tacaggtaat
    45121 actttacgtt tttttttatt atttttcaag caaataaaca aaatgctgac aatatttcca
    45181 agaaagtttc atttttcaaa ctaaaccttc tgctgccatt gctttggcaa ctttttcaaa
    45241 tccagcaaca tttgctccaa gaagcagatt tttttcgtcg ccgtactcgg ctgctgtttg
    45301 gcgacacgta tggtaaatcg tttccatgat ttgatccagc tgctcatcca cttgctctct
    45361 ttcccaagtc aatcgctggg cgttctgact catttctaaa gcagatactg ctactcctcc
    45421 agcattagca gcttttcctg gacaatacca cacgcctgct tcatgaagaa cagaaacagc
    45481 ttttactgta catggcatat tagcgccttc tgctacgatt ttgatcccat tttctactaa
    45541 gatttcggcc aagctttcgt ctatttcatt ttgagtagca catggcaaag cgatatccgc
    45601 tttttctttc aatgtccaaa cagattctcc agcatgataa atagcagaag gacgttggtt
    45661 tgcgtattcc gtcaaacgtt gtctattgat ttctttgact tctttcaata gtttcacatc
    45721 tattccttct ggatcataga tgtacccgct agaatctgaa catgtaacaa ctttcccgcc
    45781 tagctcttgc actttttcga ttgcataaat ggctacattc ccgcttccgg aaacaaaaac
    45841 tgttttttga ttaaatgaat ctttttcttc gttcaataga tgtttgacat aatacactaa
    45901 tccataacca gtagcttccg tacgtatctt acttccccaa aaatccaaag gtttacctgt
    45961 caacacacca gtatcatatt ttttcaatcg tttatattcg ccgaacaaat agccgatttc
    46021 tctagctcct acaccgatat ctcccgcagg aacatcaatc gatgggccga tatgttttga
    46081 caattccagc atgaaacttt gacaaaaacg catgatctca ttatcagatt ttcctttagg
    46141 atcgaagtca cttccgcctt ttcctccacc gattggcaga ccggttaaac tattcttgaa
    46201 aatctgttca aaagccaaaa atttcaagat acttagattg acactaggat ggaagcgcaa
    46261 tccaccttta taagggccga tagctgagtt gtattggata cggtaccctc tatttactcg
    46321 ccagtttcct tcatcatctt gccaaggcac tcgaaattga ataactcttt ctggttccgt
    46381 taaaagttct aagatattct tttcgatata ttcgggatga gtgtccaaaa aagacataac
    46441 cgtgggcatg aattcatcga ttgcttgtaa aaattcttct tgcccttgat ccttttgatg
    46501 tagcttttct tgaattgctt gaacatattc tgttgcgttt gtcatcgttc cacgctcctt
    46561 tgcttatctt ccctcatttt agctcatttt ctcttttggc tcaaaacatt tttttctgtt
    46621 ttctttcttc agatatgcta cactttcctt gaggtgaggg aatggaaaaa ctgtatgatg
    46681 tgatcgtagt cggcgctgga acaagcggga tgatggcagc gatcagtgca gctgaacaag
    46741 gcgcacgcgt actcttgatc gaaaaaaaca aaaaagcagg aaaaaaacta ctgatgacag
    46801 gtggtggtcg gtgcaatgtt acgaacaatc gtcctgttga tgatttgatt gcacatattc
    46861 ctggaaatgg aaagttttta tatagtacgt ttgcgcaatg gaataatttc gatatcatga
    46921 acttctttga atcacaaggc gtacatttaa aagaagagga tcatggacgg atgtttcctg
    46981 taacaaataa atcaaaaacg attatcgaag cattgctcaa tcgtctgaaa gaactagatg
    47041 tgacgcttct attctctact cgagtagaga aattgatcca caaagaacat aaaatttacg
    47101 gtatacgcac ggaattcgaa gagttccatg ctcctgccat tatcttaaca actggtggac
    47161 ggacttatcc atctactggt tctactggag atgggtacaa gatcgtgaag cgagtgggtc
    47221 acacggtgac gcctctttat gcgacagagt cccccttgat atcggatgaa ccgtatattc
    47281 aggagaaaac acttcaaggg ctttctttac aagacatcac tttacgcgtt ttaaataaaa
    47341 aaggtcgtgt attgactgaa catacgatgg atttactttt cacccatttc ggtatttctg
    47401 gtccggctgc actgcgttgt tcaagttttg tcaataaaga attagagaaa acgggagaac
    47461 cagtgacact ttctctggat tgtttcccta cacaaaccaa acaagagctt attcatatat
    47521 tgacagaaaa aagtaaagca accaaaaaga acttggtcaa tgcttggcat ggtatattgc
    47581 ctgagcgatt gttagttttc tttcttgagc ggcttgaaat ggatcatctg acaggacaac
    47641 aagcctctca aaaacaaatt caagattttg ttcaactatg caaagagttt aagctatcaa
    47701 tcaacaaaac ttttcctatt gagaaatcat ttgtaactgg cggaggggtt tctctaaagg
    47761 aaatttatcc taaaacattg gagagtaaaa tcattgatgg tctgtatttt gcgggagaat
    47821 tactggatgt caatggttat actggcggct ttaatatcac agcggctttc gctacagggc
    47881 atgtagcagg aatgaatgcc gggcaaagag cataatgtat aaaaaagaaa gaaggagact
    47941 ggacaaacaa catcccggcc tccttctttc ttttatacgt atgatttatt gttttatatc
    48001 tgcaaattgt ccgctcgtta atgcacagat atcttgaggt gctaactcca tctgtagccc
    48061 tcttctgcca gccgagacaa tgatttgttc atagctttct gcaatttgat ctaaataagt
    48121 aggaaatagt tttttcatac ctataggcga gcagccacca cgtatgtatc cggtcgttgc
    48181 ttccaaatct tttaaatgga gcatttctac ttttttattc cctgaaactt ttgctaattt
    48241 ttttagattc aattcggctt ctcccggtat cacagcaaca agaggccccg tcacattgcc
    48301 cacagcaacc agtgttttaa atatatgaga ttggctttct ggaagctgtt cagcgaccga
    48361 tgacgcactt aggtggcttt cgtcccactc atattcgtgc tctgtatagg gaatcttttt
    48421 ttgttcgacc atacggattg cattcgtttt tactattttt ttctttgcca aaaaatatca
    48481 cctcaaactt gttctacaac ggttcattta ataattgaat aatttgcttc aatgctttga
    48541 ccggtaattg tcctggagca gaagtagaat cgctgcttaa ggaaccaaat gtcataacgg
    48601 aacccgtcgt tccgccagcc agacgagaaa ttttaccaag atttcccatg gacatcgtaa
    48661 taataggcat ggagacaaaa gcatttgccc gtctagtcaa ctccatcaac cgcaaaacat
    48721 ctcgttcatt attgggagtt actgccagtt ttccgatatc ggctccaaaa tgttccataa
    48781 cattcaatcg gtaaagcaag acagggtctg ctggtgtttc tttaaaatca tgactactga
    48841 taattaaagg aatgtttaaa gacttgattt cctctatcag tctttttcct agactttcta
    48901 ctttgaacaa ctcgatatcg accatatcta ctaatcctgt gtgtatcaca tgttcataaa
    48961 ggtcgcgata ctgacggatc gatatgtctt gttctccgcc ttcagctaag gtacgaaacg
    49021 taaataataa cggcagtcca tcaagcatct catggataag aaccaatgtt tctgctactt
    49081 catcaaaaga aaaaacttca cgataatgat cgactcgcca ttcgactaaa tcggcccctg
    49141 cttcacgtgc aagaactgct tcacgtaata cttcctctcg gacggccccg ataagaggcg
    49201 cacaaatctt tggtctaccg cttcctatat tcaatccctt tacccaaacc attctgcatc
    49261 gctccttcta atggttattt ttaatttgat aaacaaacac tttcagataa ttgccttcta
    49321 aaaaggtatc cgtcgtctgg aaatcttttg gcagacgata taaagcaagt tgttcaaaag
    49381 aaacattttt ggcatggaat tcctcttcaa tcattgtttg gaattgttct atcgagacat
    49441 tcgcagcatt tgtagacgca ataagtatcc cgtcttctgc taaaatatcc agactatcct
    49501 tgaccagttc cccataatct tttgctacgc gaaagacttt tttcttattt ctggcaaaac
    49561 ttggtggatc caatacaatc atgtcatata aaaattgttt tcttttggcg tatttgaaat
    49621 actcgaatac gtccataaca atgatttttt gctgatcgac atccaaacca ttcacttcaa
    49681 actgttcctg tgtttttttt aaactgcgtt ttgctagatc gacacttgtt gtttcgattg
    49741 cccctcccat tgcggcagca acagaaaagg cgccggtata actaaacatg ttcaaaactg
    49801 ttttcccaga agcgaaacct tctgctaatg atccccgtac ttctttttgg tctaaaaaga
    49861 tcccagtcat taatccttca tttaaataaa cggcaaattt tactccgttt tcttttacga
    49921 tcaagggttc cggtgcctct tttccgtaaa tgaactggct ctcaggcaga tttactgatt
    49981 caaatcgaat tttctcatag cctcctacga tttccgggta gatttcacga aaagcttgaa
    50041 tcaataattc acgatgtgca tataacgttt cgttatacca agaaaaaaca gcataacctg
    50101 cataataatc aacaatcaga ccgccgatcc cgtctccttc tccattgaat atacgaaaag
    50161 cggtagtaga atcatctgaa aaaaagaaag ttcttttttc ttttgacttc acaaaaaatt
    50221 cttcaaaaaa agattgatca aatgggatgt tctgctggct caatacccaa ccgattccct
    50281 tattctgctt tcctagatat ccttttgcga taaattggtt ttgcgaatca actaattcca
    50341 cccattgcgt ggtttctttt tggggatgag caaggtcttc tttttggatc agtggatatc
    50401 cttttttcaa tcgattgctt gctctttttt ttacttttac tttcattcct tcacctgctt
    50461 tagctttctc ttcccaagta taacaaaaaa cataggactt gtcttactgc tcattttttc
    50521 atgaagctta aagaaaacct taatgaaaca gcataaactt ctggtttcaa ttgacactat
    50581 attgtcatac tagtaaaata aatgagaata agtgagctgt gtaacatggt ttcatgacac
    50641 ttctgtgatt aagaagtgca gaaaggaaga ttatcttgaa atgtataaaa acacctgcct
    50701 ttttgaataa gaggctcgga tttttttctt tactagttgt tttactttgg ttgaaaacga
    50761 tttttgccta tttagttgat ttccatctcg gaatcgaaag tggcattcaa tactttatct
    50821 tgtttatcaa ccctattgca actacattgt tattacttgg aattgctctt tatatcagac
    50881 gccctagagt tgcttatatc acgatgatgg tcatttattt tcttttgaca ttgcttgttt
    50941 tttcgaatgt cacttattat cgagaattca cggacttcat tacgatcaac acgattttag
    51001 gtgctgggaa agtagcgagt ggtctgggag aaagtgccat tcgactgttt cgcccatatg
    51061 atgtgatcta ttggatcgat tttattatct tgactgttct gctgattgca aaaaaaatca
    51121 agttggaccc tcgcccgatc cgagcaagaa tggcttttgc gatctctaca ttagcagtta
    51181 tgattttctc aggaaatctt tttatggcgg aagctgatcg tccagaattg ctgactcgaa
    51241 cattctctcg tgattatttg gtcaaatatc ttggcttgaa tgctttcatg gtttatgacg
    51301 gtgttcaaac ttaccagaca aaccaagtgc gggcagaagc aagtcccaat gatatgaaag
    51361 aagtagaaaa ttatgtaaaa gaacattatg cagcaccaaa tgatgatttg ttcgggctcg
    51421 ctaaaggaaa aaatgtgatt tatatccatt tagaaagctt ccagcaattc ctgatcgatt
    51481 ataaattgac agatgagaat ggtctccagc ttgaagttac acctttcatc aatagtcttt
    51541 accacagcaa cagcacgttc agttttgaca actttttcca tcaagtaaaa gcaggaaaaa
    51601 caagcgatgc ggaaacgctg atggaaaatt cattgtttgg cttgaatcaa ggctccttgt
    51661 tcacacaatt aggcggaaaa aatactttcc aagctgcacc tgatatttta agtcaaacac
    51721 aaggttacac tagtgcggta ttccatggga acgctggtac attctggaat cgaaacgaga
    51781 catacaagaa tttaggatat gattatttct ttgattcttc ttattatgat gttaatgatg
    51841 acaattcgtt ccaatacggg ttgcatgata aaccattttt ccaacaatcc gtacaatact
    51901 tggaacgtct gcaacaacca ttttattcaa aattcattgc tgtatctaac cattatcctt
    51961 attcaaaatt caccaatgac gaagcaggat tcccgttagc caatacaaat gatgaaacga
    52021 tcaacggtta ctttgcgaca gccaattatt tagataccgc cgtacaagaa ttcttcaatt
    52081 atttgaaaga aagcggcttg tatgataatt cagtcatcgt cctttacggt gaccattacg
    52141 gtgtctcgaa ttcaagaaat aaaaatctag ctgaattagt tggaaaaaca agttctactt
    52201 ggacaaacta cgacaatgca caaatgcagc gtgtccctta tatgatccat atccctggac
    52261 aaacacaggg cggaatcaat cacacttacg gcggacaagt tgatgccttg cctacattac
    52321 ttcacttact aggtgtagat acaaaaaatt atattcagtt aggacaagat ctattctcga
    52381 aagatcatga acagcttgtt tctttccgtg atggtgattt tgttacacca aaatatactt
    52441 actacggtgg tacgatctat agtaacgaaa ctggtgaagc tattactgaa cctactgagg
    52501 aagtacagaa tgaaattgat tcactgaaag aaaaagttga taaacagtta gctgtttctg
    52561 accaaatcaa taatggtgat cttcttcggt tctatacgaa tagtggatta gacccaattg
    52621 ataccagtga ttatgactac aagaatggct tagagaaatt aaaagaagct gaaaaatctc
    52681 ttggtgacaa gtcaactagt gtctttagtg aacacaaggg acaatcaact gttgatcttt
    52741 ataaaacgca aacgtaccaa gaacttcacg gaacgaccac aagttcttcc gaatgataaa
    52801 ataaatttaa aaggagcagc gacaaaatta ttgtcgctgc tcctcttttc gaataaacag
    52861 tgttcaaaag cttgcatcta cggtaataga atagggcagc ttcttcacaa cctcttgctt
    52921 tttacaaaat attttctggt tttaattcgt gatttcacta aaggaatttc atactttatt
    52981 catattcatt ttctatactt tttatagagg tgaaaagtat gaaaataatc cgctatgcta
    53041 ctgctcgatt aatttattac aaaaagcaga caatactcta ttgtttattt tcaagctttt
    53101 ctgctttttt attgatagca tgcctgactc tttaccattc gcaatcggct ttagccaatc
    53161 aagtccaaga tcggctttca tttattggcg cttctgcatc tacagagctt cttccggctt
    53221 tagaattagc tagccgattt tatataaaag gcatattcag tttatttttt ctatttgcct
    53281 tcctttttat ctttttcttt tactattcag tcaggcaaaa caagcaggaa ttgatcaatt
    53341 ggcgcctgac tggtctttct aaaaccaaat tattcctttt tattttctgg cagctgttct
    53401 ttcctctctt tctttgttgt atcttggttt tattgtggat cgtcagcttc cagtcagttt
    53461 atgaagcgct tcttcaaagg atcaattttc ttgtcttaaa gactactgaa ctgccagata
    53521 tccgtgatat cttgacttcg gtaagcggac ttacaattcc gatggatcaa cagacttttt
    53581 ttaccatcga tttccaaaat gatctttttt tcacagatat acttaaggga tttgcccaaa
    53641 ctcttattgg gctcagtgga tgctgtctca ttcttagtct attccctttc acttttttta
    53701 tccatcattt gcaaagtagg aggcctggac gtaatgaaac ctgaatattt gattcatgct
    53761 gaacacttga ccgttatttt gtgtcctctc gaaaaacagc ataaaatatt ggagcacttg
    53821 aaaaaagagg ttcttgttgc aaaactggat aattttgcga tcatccaaaa aaattggccg
    53881 atgtttcctt atctgaatct taaagaccat gtcctgttgg atgtaccgga aaaagaaatc
    53941 aaacaagatc gtttggccta tcaagaaaaa cttgatatct ctccttcctt gcttaattgt
    54001 gcggcagctg agttgacgtc ttttgaaaaa gtaaagctgc aattacttca tgccttgctt
    54061 tccaagcgaa acaatattgt tattgaagat acatttgatg aacttagtgt attggagatc
    54121 caagagctac tccacttgct ttcttattta gctcatgaag aaaatcaagg tatcttactc
    54181 tttactcatg atgctactat tgctcaaagt ccttatattg atcgtttaga gcccgcgggc
    54241 taaatcaatc attttatagt aaaaacaagc ggtatagaag atcgattcat gatcttttat
    54301 accgcttgtt ttttactctt ttttgatttc tacgtcaggc aaacgaacag cgaattttgt
    54361 tccttttcct agttcgcttt ctacttctaa ttttccttta tgcaagcgta caagttgctg
    54421 cacgattggt aaacctaatc ctgattcacc atattttgta ttttttcttg aaggatctac
    54481 tttataataa cgatcccaga tacttttcat ttgttgttca gacattccaa ttcccgtatc
    54541 ttcaatcgtg atgatcgttt caagatagcc tttttctaaa gtaatcgtga tttggccgtt
    54601 ttcagtaaac tgtatcgcgt tttgaataat attgaccatg atttggacaa atcggtcata
    54661 atcagcgtaa acatcaatat catcaacggc tttcaatgtc aatgtatctc ctgctgcttc
    54721 tgctttagct tctaactgtg caacaatatt tttcaatgtt tctgttccat tgaatttttt
    54781 aatgaccata ctgatttgat tcgtacggat tttttcataa tccaagtttt cattcaccaa
    54841 acggatcaga cgctctgttt catttttcat caatttaatc gcgttttctc gctgattttc
    54901 agggattgca ttgtattcta acccttccag aagtccgttg attgtcgtaa gaggtgttct
    54961 catctcgtga gaagcatctg ccataaattg tcttcgacga ttttcttgtt cttcaatttc
    55021 tgcttgagat tctttcaatg aattcgtcat tttattgaaa tcatctgcta actcatcaaa
    55081 ttcatctttg tcatgtacgg ggagctgtac atcaaaatta ccattcgtca cttctttcgt
    55141 cgcattcctt aaccgattga tacgcttaac ttggaatgct gcaaaagcat agctggcaat
    55201 aactgcaacg atactggaaa aaataaatcc tttgaacagg ttcagtgtaa ttggacgaac
    55261 gctgttatcg atatttcgcg caggttgact aactacaagt gctccataga attcatgatt
    55321 caaattaaat ggcacaagtg cgtaagaggt cgcctgtgct tgtcctaaaa tatttttatt
    55381 cgatgtgaat ttttcttgac gtccattcct cagatttttc cattgatcag ctgttaaggt
    55441 aaaattcagt aaagctgtat tagtcggata aatgacacgt tcgtttttat cgataaatat
    55501 aaagttcaca ttttgttctg tcagtacttg ctctgtcagc agcaatgcat tttgaaacgc
    55561 ctgatcttga ctaaactgcg ggagcgtatc ggcataggtt tgcgttgtct tttcaactga
    55621 ttcagcatat ccaaacagtt gctggtaatt gttttcttca atcgtttgtt ttgtcagttg
    55681 tgtaaaagaa actccaacga tcaataagat caacgcgacc attccgcaaa aagctaagag
    55741 ttgctgatac aaataacgca ttaggcaaca ccagaatcat cgaatttgta gccgactccc
    55801 caaacagttt ggatgacttg tggtccgacc ttttcgattt tttgtcgtaa tttcttgata
    55861 tgggcgtcca ctgtccgttc atcaccaaag tactgataat cccagactaa ttcaagcaac
    55921 tgctcccgag agaatacttg acgtggtttt tttgctaaag taaacaatag atcgaactct
    55981 ttaggcgtca gtccttcaat tggccggttg tccagatacg cttctcgtgt tttcgtattc
    56041 atcttgaaat gactagtctc gatatcaaaa tgatcatctg aaactgctgc ttcttctctt
    56101 gcttctgcca actcactacg gcgatgcaaa gctttcattc gagcaataag cgtcagagga
    56161 ctgaaaggtt ttgtgacata atcatctgcc cccatctcta agccgatcac ctgatcgctt
    56221 tcagaatctc tagctgtcag catgataatc ggcacagtac ttgaaacttt gcggatctct
    56281 cttcctacta gcataccgtc cagtgtgggc aaattcaggt ctagtgtaat catatcccaa
    56341 tgttgtgggt tttctaaaaa ggcatcgagc ccttcttttc catcatactt gaaagttgct
    56401 tcccaacctt catttaagaa aaacatctgc atcatttcag aaacagattg attatcttca
    56461 atcattaaaa tattcattat cgttccctcc agtttccagg tcatatttct gtaatttctt
    56521 ttctgtcata ataaaaatag gcataattgg aaatactagc agaagcattt caatcagaaa
    56581 ataattcgta tgcccagatg tctttaaata atagccgatc aatgccatca gtaagcccat
    56641 aagaaaaaac cagtttccgc ttgtcttttg agccagccgc caatgagcgt ctgtttttgc
    56701 ggcaagaggc gaatgatacc cataaaaagg cagatttcct tttgagggca gaatccgaaa
    56761 aagactgcca attattaaga ataagatacc aatactgaaa aaaatcattc tttccctcct
    56821 aaattctctt cttatagtat actctaaatt gttgactgaa ccaatatgtt caggaattct
    56881 tcacgaaagg tttgtctttt gttcattctt tgttaagata aacttattcc aataaaaaag
    56941 gagttttatt tgtgtcaaat tatcgtgcaa tcactttctt tgatcttgat ggaacattac
    57001 tagatgcgaa atcaaaaatc acacccgaag tggcaaaagc tatgaacgca ttaaaagaaa
    57061 acaatgttct ccccgtcatc gctacaggac gtacagaagc agaaatccac tctatcatgg
    57121 aagatgcggg cattacttct gcggtcacca tgaatggttc tttcattcga gtagacggcg
    57181 aagaaatcta ttctcaaaca ttttcttcag aagaatgtca aaaaatgtat gatcatgtac
    57241 agttgaacgg tcatgaactt tcttactata acgatcagaa aatctggtgt accggtcata
    57301 gccaaacaat gatcaatgct tacgacttca ttcattcaga tgttccggaa attgatcctt
    57361 taggctttaa agaaaacaca gtaaatatgt tgctgatctt atctcaatca ggtgacgaat
    57421 actatcacga acattttcct gagctaactt tttatcggaa tggtccttac tcgatcgata
    57481 cagtgaagaa aggaatctca aaaggggcag gtgtcaaacg tctgcaaaaa gaacttcagc
    57541 tggaaaaagt tcctactttt ggatttggcg atggtccgaa tgattttgcc ttgttggaag
    57601 cttgcgacaa caaaatcgct atggacaacg cctatgacgg attaaaagaa ctttctacct
    57661 ttatcacaaa gaaaaataca gaaggcggaa ttgtccacgc attgaagtat tttgatctga
    57721 tttaagatct ttttttgaag ctacctcttt tcttaagaaa atcgcttatt ttttcttaag
    57781 aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg aatttatccc
    57841 aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga
    57901 cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt
    57961 aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc
    58021 ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa
    58081 ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag
    58141 cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat
    58201 gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat
    58261 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
    58321 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
    58381 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
    58441 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
    58501 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttta attattattt
    58561 gtcaaattaa gcgagacaaa actttccatt tacgtaactc aaaaaaactt ctaatggtgt
    58621 ttggtaattt agtgattttc tagggatatg gtttcttttg gacgcaacgg atgagatgaa
    58681 tccttgattg acttggtgga agtccatttc ttttggtagt ccatcttttc ggaggagtcc
    58741 gtttgaatgt tcatttaatc cccgttggga aggcgttcct gggtctgcga aataaatatc
    58801 aatatcattc gtattactaa tact
//
LOCUS       DACDLI010000010        51279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDLI010000010 DACDLI010000000
VERSION     DACDLI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.010"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            199..723
                     /locus_tag="IV353_000655"
     CDS             199..723
                     /locus_tag="IV353_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008927.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            739..2238
                     /locus_tag="IV353_000656"
     CDS             739..2238
                     /locus_tag="IV353_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9008928.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(2326..2928)
                     /locus_tag="IV353_000657"
     CDS             complement(2326..2928)
                     /locus_tag="IV353_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9008929.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            3032..3988
                     /locus_tag="IV353_000658"
     CDS             3032..3988
                     /locus_tag="IV353_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9008930.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(4293..5249)
                     /locus_tag="IV353_000659"
     CDS             complement(4293..5249)
                     /locus_tag="IV353_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9008931.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            5423..5983
                     /locus_tag="IV353_000660"
     CDS             5423..5983
                     /locus_tag="IV353_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008932.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            5976..6800
                     /locus_tag="IV353_000661"
     CDS             5976..6800
                     /locus_tag="IV353_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9008933.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            6810..7427
                     /locus_tag="IV353_000662"
     CDS             6810..7427
                     /locus_tag="IV353_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9008934.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            7445..8128
                     /locus_tag="IV353_000663"
     CDS             7445..8128
                     /locus_tag="IV353_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9008935.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            8811..10094
                     /locus_tag="IV353_000664"
     CDS             8811..10094
                     /locus_tag="IV353_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9008936.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            10110..10997
                     /locus_tag="IV353_000665"
     CDS             10110..10997
                     /locus_tag="IV353_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9008937.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            11014..11850
                     /locus_tag="IV353_000666"
     CDS             11014..11850
                     /locus_tag="IV353_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9008938.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            11853..12476
                     /locus_tag="IV353_000667"
     CDS             11853..12476
                     /locus_tag="IV353_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008939.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            12473..13765
                     /locus_tag="IV353_000668"
     CDS             12473..13765
                     /locus_tag="IV353_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9008940.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      14124..14221
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            14337..14918
                     /locus_tag="IV353_000669"
     CDS             14337..14918
                     /locus_tag="IV353_000669"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9008941.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            14921..16228
                     /locus_tag="IV353_000670"
     CDS             14921..16228
                     /locus_tag="IV353_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9008942.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            16644..17132
                     /gene="purE"
                     /locus_tag="IV353_000671"
     CDS             16644..17132
                     /gene="purE"
                     /locus_tag="IV353_000671"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9008943.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            17116..18249
                     /gene="purK"
                     /locus_tag="IV353_000672"
     CDS             17116..18249
                     /gene="purK"
                     /locus_tag="IV353_000672"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9008944.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            18262..19557
                     /gene="purB"
                     /locus_tag="IV353_000673"
     CDS             18262..19557
                     /gene="purB"
                     /locus_tag="IV353_000673"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9008945.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(19737..20114)
                     /locus_tag="IV353_000674"
     CDS             complement(19737..20114)
                     /locus_tag="IV353_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008946.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            20370..21098
                     /locus_tag="IV353_000675"
     CDS             20370..21098
                     /locus_tag="IV353_000675"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9008947.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            21098..21352
                     /gene="purS"
                     /locus_tag="IV353_000676"
     CDS             21098..21352
                     /gene="purS"
                     /locus_tag="IV353_000676"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9008948.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            21354..22025
                     /gene="purQ"
                     /locus_tag="IV353_000677"
     CDS             21354..22025
                     /gene="purQ"
                     /locus_tag="IV353_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9008949.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            22026..24248
                     /gene="purL"
                     /locus_tag="IV353_000678"
     CDS             22026..24248
                     /gene="purL"
                     /locus_tag="IV353_000678"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9008950.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            24233..25672
                     /locus_tag="IV353_000679"
     CDS             24233..25672
                     /locus_tag="IV353_000679"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9008951.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            25704..26747
                     /locus_tag="IV353_000680"
     CDS             25704..26747
                     /locus_tag="IV353_000680"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9008952.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            26744..27322
                     /gene="purN"
                     /locus_tag="IV353_000681"
     CDS             26744..27322
                     /gene="purN"
                     /locus_tag="IV353_000681"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9008953.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            27319..28854
                     /gene="purH"
                     /locus_tag="IV353_000682"
     CDS             27319..28854
                     /gene="purH"
                     /locus_tag="IV353_000682"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9008954.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            28870..30144
                     /gene="purD"
                     /locus_tag="IV353_000683"
     CDS             28870..30144
                     /gene="purD"
                     /locus_tag="IV353_000683"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9008955.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            30266..31180
                     /locus_tag="IV353_000684"
     CDS             30266..31180
                     /locus_tag="IV353_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9008956.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(31313..32878)
                     /locus_tag="IV353_000685"
     CDS             complement(31313..32878)
                     /locus_tag="IV353_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9008957.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(32871..34322)
                     /locus_tag="IV353_000686"
     CDS             complement(32871..34322)
                     /locus_tag="IV353_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9008958.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(34336..35352)
                     /locus_tag="IV353_000687"
     CDS             complement(34336..35352)
                     /locus_tag="IV353_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9008959.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            35572..36660
                     /locus_tag="IV353_000688"
     CDS             35572..36660
                     /locus_tag="IV353_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9008960.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            36714..38240
                     /locus_tag="IV353_000689"
     CDS             36714..38240
                     /locus_tag="IV353_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9008961.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            38242..39447
                     /locus_tag="IV353_000690"
     CDS             38242..39447
                     /locus_tag="IV353_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9008962.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            39704..40681
                     /gene="guaC"
                     /locus_tag="IV353_000691"
     CDS             39704..40681
                     /gene="guaC"
                     /locus_tag="IV353_000691"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9008963.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            40945..42369
                     /locus_tag="IV353_000692"
     CDS             40945..42369
                     /locus_tag="IV353_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9008964.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            42830..43939
                     /gene="pdhA"
                     /locus_tag="IV353_000693"
     CDS             42830..43939
                     /gene="pdhA"
                     /locus_tag="IV353_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9008965.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            43942..44919
                     /locus_tag="IV353_000694"
     CDS             43942..44919
                     /locus_tag="IV353_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9008966.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            44944..46587
                     /locus_tag="IV353_000695"
     CDS             44944..46587
                     /locus_tag="IV353_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9008967.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            46595..48001
                     /gene="lpdA"
                     /locus_tag="IV353_000696"
     CDS             46595..48001
                     /gene="lpdA"
                     /locus_tag="IV353_000696"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9008968.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            48163..48438
                     /locus_tag="IV353_000697"
     CDS             48163..48438
                     /locus_tag="IV353_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9008969.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            48444..49211
                     /locus_tag="IV353_000698"
     CDS             48444..49211
                     /locus_tag="IV353_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9008970.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            49379..51208
                     /gene="typA"
                     /locus_tag="IV353_000699"
     CDS             49379..51208
                     /gene="typA"
                     /locus_tag="IV353_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9008971.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT        16603 a         8838 c        11435 g        14403 t
ORIGIN      
        1 gttttttctt aaaaaaagga aacataggct acctcgaaag aatttattgt gaaaatttta
       61 acatgaaaac aaaatttaac aaattgtcaa cgataaacac aaccttccta taaaaaacac
      121 cactgagtcg gtaaaagaag ctatacagaa ttttcaaaca ttggtatgat agacgtagaa
      181 atggaaggag gcgtgtatat gataggggga atcgcacttg agacgattta ttggtataca
      241 ttgctgattt gtgcaggatt ggcagtccta ttgattattt ttggagatgt attcgatttt
      301 gatggcccgc ttgatccgat gcttattatc ccttggttaa ctttcacaag cttatttggt
      361 tttctaggtg agcgtttaac agatcaatca tctttatttt tatttattgc tagtggaggc
      421 atatcgatag tgttggtatt tttgcttaat ttctatatat tgatgccgat gaaacatgcc
      481 gaatcaacga tgtcgtcttc tgaaaaaact ctggaaggac aagtagctgt tgtgatcaca
      541 ccgatccctg tacgaggtat gggagaaatt cagttgaaaa gtgttaccgg ttccattagt
      601 cgtccagctt gtttttacag cccccaagaa cagacagctg agcggggaac gaaagtattg
      661 atcattgaga tcaaggaaag aatctgttat gtcatgcctt atgaaagcat gataaaagct
      721 taaaggagcg tgtgttaaat ggaaatacca gttgttttta tcgcgatcgc aatcgcagct
      781 ttgattttac tgatgttgct gatcgtcttt gtagcgaagt accagacagc aaaaccggat
      841 gaagcgttga tcatcagtgg aagttattta ggaagcaaaa atgtgcacat tgatgaaagc
      901 aataacaaaa tcaaaattgt gcgtggaggt ggcgcattcg tcttgccagt ttttcaacgt
      961 tcgaatcgga tcagtctatt atcgagtaaa ttggacgtct ccacccctga agtgtatacg
     1021 gaacaaggcg taccggtgat gtgtgatggt acttcgatca tcaaaatcgg ttcatctgta
     1081 gaagagattg ctacagcagc agaacagttt ttaggcaaga caagagaaga actggaaaat
     1141 gaagcaagag aagtattaga aggacacttg cgctcgattc ttggttctat gactgtagaa
     1201 gaaatctatc aaaatcgcga taagttcagt caaagcgtac aagaagtcgc ttctgtagac
     1261 cttgcaaaaa tggggttgat catcgtttct ttcactatca aagaggtcag agacaaaaac
     1321 ggttatcttg actctttagg gaaaccaaga atcgcccaag tcaaaagaga tgcagatata
     1381 gcagaagcgg aagctctaaa agaaacgcgt atcaaaaaag ctcaagcaga aaaagaatca
     1441 caagcagctg aattacagcg acaaacagag atcgcagaat cattaaaaga aaaagaactg
     1501 aaattagcaa cctacaaaca agaacaagat gttgctaaag caaaagcaga ccaagcctac
     1561 aatttagaaa gtgcccgagc acaacaacaa gtaatcgaac aggaaatgca gattaaaatc
     1621 gtagaacgtc aaaaacaaat cgaactggaa gaaaaagaaa tcacacgtcg tgaaaagcaa
     1681 tatgattcgg aagtgaagaa aaaagcagat gccgatcggt acgctaaaga acaagaagca
     1741 caagctcaaa aagtcaaaga agtgactgaa gccgaagcag aacgattccg tgtggaagca
     1801 ctggcagaag cggaagccaa taagacacgt ttagctggac aagcagaagc cgaagcagca
     1861 ctagcaaaag gaaaagcaga agccgaagca aaacaaaaga tcgccaatgc cttcaaggaa
     1921 tatggcgaag cagctgtctt gagtatggtt atcgatatgc taccgcaatt gatgcgcgaa
     1981 gcagcacagc cgctaggaaa tatcgaaaaa atctctgttg tcgatacagg aagcagttca
     2041 ggagaaacca gtggagcgaa ccgagtgaca aattatgcca caaatttgct ttcttctact
     2101 caagaaacac taaaagcgac aacaggactt gacttgaagg aaatcgtaga gaatttgtcg
     2161 acacgtggga ctactagcca agtaaatatg tatgaagcgc caaaagaggc agaacaaaac
     2221 tcgatgaagg aagaataaag tagtacactt caccgttcaa tcaaaaatgg aggaagactt
     2281 tttaaagttc ttctctccat ttttttattt tattgcaaaa gaaaattagt cctcgctatt
     2341 tttcttttgt ttttggatct tagcttctat ctcttgtaaa tttgttctta gcaatcgttc
     2401 tttcttttgg agttcgacca aatcttctga tagttcttcc ttcttttctg tttcaggagg
     2461 acgaagaaag aaattgatac cgattgcaat gaatgtccct ataaaagtat ctagtacacg
     2521 ttggatagca tacaaagcgg attcgccttg agggatgctc aacgcaatca gcaacaacgt
     2581 agctattgcg gaaatgatac cggaattatt gttaatacca tctgacacga cgatcacaat
     2641 aataactaga ataggaagca agatcaattc taccaaaaaa tcattgtgaa aatagctttt
     2701 gacaaaaaaa taaatgatag ctaatgtacc acctaatgta ttgccgatga tcctagactt
     2761 tccaaaagaa acggttgtag tcaagtcctg acgcaaagaa aaaacagcag ataatgcagc
     2821 aatcatcggg attcctcgtt gagtcaaatg aaaaaacaag atacataaaa aaacagctaa
     2881 ggcacttttg atcgtacgca tacctagtcg aaaacggcca aattgcatag agattctcct
     2941 ttttggtctt tttactatca tacctaaact tttgtctgat agctagtttt tcattattca
     3001 agtataatta aaataaaaaa gggggaagta aatgcgtata ggaatgcgaa cagtcaaaac
     3061 agtgatcagt gtcttctttt cgatggtcac agcgggtagc ttatcgctgt tgtattggcc
     3121 agctgcaggg atcattgcat tattaagtgt aggaaatacc aagcgctcta cattgcgtac
     3181 gggcatgaat cgaatcgcag cctttatatt agctacggca acagcactgt tcagtttttt
     3241 tgctgttggg tatactattt ttggcttcag tcttttttta ttcctgttca tcccgcttgc
     3301 ctctcgcttc aaattaaccg aagggatagt agtaaattcg gttttggtca ctcattattt
     3361 agtggaggaa aacatgtctt ggcagctgat cggcaatgaa gcgggattaa tggcgattgg
     3421 attagtattt gctttgcttg caaatgttta catgccagat cgtacgaagc agctgaagga
     3481 aaaccaaata cagatcgaaa aagaatttcg aaatattttg agacaaatgg cagaatttct
     3541 tttatcagaa aataaagaag atgtgcagat caaatgcgaa catttactga cattcattcg
     3601 cgaaagccaa gaagatgcca gagaacatca agaaaactac tggctcagac agccgctata
     3661 ctacgaaact tacttctcta tgagaagagc acaggccaat gtgattaaag acatgctgga
     3721 aaatctagag agaatccagc aaccagctta ttatggaaaa catatctatg gtttattgat
     3781 ttatactgca gaaacatttt cagagtcaaa tgatggcaga caaattttaa cacgtattga
     3841 agaagtttat gtattgtatc ggcaaatgcc tctgccgact agtcgccctg aattcgagga
     3901 tcgagcagaa ttgttccagt ttttgcagtc ttttaaaagt tttattgaaa tcaaggcaga
     3961 attctcccaa caaaaaatcc aaaaatagag gttgtgaatt cagctaatat tcaggtcaaa
     4021 aagctgattt cacaacctct tctttaaacg gtgttctcct gtcaactcct cggtcacaag
     4081 tcacaatatt caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcggagc
     4141 tgaacggtag gctcacaacc tctttcttaa acggtgttca aaagctttcc tctgcggtaa
     4201 taagtccagc caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg
     4261 atgcagaaca gctttttcac aaccttttaa aattacatat ttttccatag aacttgcatt
     4321 tgatccaaga tttcgatgac atcttttgtc aagaagagag agcgattggg caagtcacct
     4381 tcaatcatgt gtaccatatt ctttatttcg taattcattg cttcagatgt attcccgctt
     4441 tcgatgaatt cctttgtacc atctgtaaat aagatctccg cacgatcagc acgcgggtaa
     4501 tcagcaatcg tcacataacc gttctcaaag gcaacgatac cgacttttgg catttttgcc
     4561 tgaaaagtca agctgacagc agcaagttca ttttctttgt tacgcaagat agtgacggat
     4621 tgttcgtcca cgcctgtttc aaaaggaacc attgtagagg caactacttc aggttgagag
     4681 ctcaaaaaga aacgagcgaa agatacagca tacgtaccga tatccaacag agctcctcca
     4741 gctaattctg gattgaagaa ccggttttta gggtctggtt ctttgtaact gccgaaaggt
     4801 gcttggatca tttttaaagc ccccagtttt cctgtatcca ttattgagcg taactgctga
     4861 taaagtggca tgttgaagat tgtcatcgct tctgctaaga tcaaattctt ttcttctgct
     4921 aagcgcatag cgtcagccag ttctttttta ttcatcgtga tggctttttc acatagaacg
     4981 tgtttgcctg cttccaatgc tgccaagata tggtcgatat gctgtctatt tggtacagca
     5041 atgtacacga tgtcgatttc agcatcgtct aacatttctt gatagcttcc gtaagctttt
     5101 ggaatattgt aacggtgagc gaagttttcc gctttttctg aagttctgga agcaactgct
     5161 gttagttcgc tcgtctcttg attgaagctt tcggcaaact catgtgcgat ggtaccaagc
     5221 ccaacgattc cccattgata ttttttcatc tgaaaacgct ccttcatagt tggattgagt
     5281 ttcttgtctg tctattatta taacaaaaat cacgaagaat aaagaggaga ggacttgaaa
     5341 cattaaaaaa aatgaaagca gcgatttaag aggtgctttt tgatcaaaac ttaagtagaa
     5401 tagttaaaag gaggggataa acgtgcatca agtatatatt gatcttatta tgagtgtatt
     5461 attagaacag tttgaaactg aaaaaaattt tttagatgaa tacttgctga tcagtccaga
     5521 acaatggcaa aaatggaaag atggcgagaa aaatctgcca tctgaagcga tgcagaaagt
     5581 aaaaaatctt ttcactgatt atgaatggat gctaacgcaa aagattttgc gtcaaacgat
     5641 tatttttccc gaaaaacgaa acgttgccgt tgcagagtac aagcaaatca aaacacgcat
     5701 cgcgcaaaaa tggcttagca gcaaagcagc aaaagtcgag ctgatccctt tgaaaaataa
     5761 tcagcaggct ggtagatacc ttgacttgaa agtaagtatt caatatgatg aatggggcta
     5821 tgatgatatt ttgaattttc gtctgccagc agatgttcaa aaacagatcg aaggagaaaa
     5881 aatcgaactt ctcgattggg tgaatgaaaa tctggaagaa acttacgttc atgaggcgaa
     5941 tggggaacgt gaagaacaac gaaaggattt ttcaagtgaa taaattttta ggatttggag
     6001 cagttgtgat tatgatcgca gcaatgggct atgttatttt tagcgatgat gattcgcatg
     6061 ttcaagccgg caatcaagca gtcgaacgaa caagcaccaa aacaactgct agtaaagaaa
     6121 agaacagtca aacaaccagc agcaaggtaa aggacaaaga aacaaaagat cgtacgtcca
     6181 aacaattgcc agatgttaaa acagacgatt ggaagctagt tttggttggt ccagatcaca
     6241 aattagaaaa agaaattgat gagtctactc aattggcatc attaagtaac ggatacttga
     6301 tcgacaaacg cattgctcca gaatatgaag catttgccaa ggctgctgaa gcagctggat
     6361 ttccattagt gatggtctca gcttaccggt ctgtatcatc ccaacaacaa gttttcgaac
     6421 aaaatgtcca agatgtgatg agtcgtcaag gtgtatcaga agaggaggcg acaaagatta
     6481 ctaaagaaac gattacggag ccaggataca gcgaacacca cacaggtctg gcagtcgatg
     6541 tcgtcgatca aaactggtac aacagttatc cttctactgt attggatgca agttacggag
     6601 atcagcctgg agctaaatgg atcgcagata atgctgcgca gtatggattt atcgttcgtt
     6661 atccaaaagg acacgaagat ataactaaga tcacatatga accttggcac ttacgttatg
     6721 tcggtaaaga aaacgctgag tatatcacca aacaccaatt gacgctagaa gaatatctga
     6781 atgaattaaa tgaaaagtag gaattgatca tggcgaaaaa aaagtggaaa aagcaaaagc
     6841 gcaagaatat ccgttggcca gcagtggttg ccggtgtagc agtgatactc ttagcctttg
     6901 ttttttcctt gaagaatctg tcttctcctt ttactgataa tcagcaagaa aatgaagcgc
     6961 aatcccatca gcaatttatt gaacggcttg ttccgcatgc ccaagaacta caagatggtt
     7021 atggagtgct tcctagtatc attttaggac aagctatatt agaatcaaat tggggaaaaa
     7081 gtaccttggc tagcaaatac aataatcttt tcgggatcaa ggcttatggg gatcagaaga
     7141 aagtaagcct ggaaacaaag gaatttgtta acgaagaatg gataactatc caaggagact
     7201 tcaaagtgta tgattcatgg gaacagtcca tggatgacca tacacaactg ttcgtccaag
     7261 gagtagattg gaatcctgcg ctgtatgaaa aagtgattac ggccaccaac tatcaggaag
     7321 ccgcacaagc attgcaagat gcaggttatg cgacagaccc cggttatgcg caaaaaatca
     7381 tccaggtcat cgagacgtat caattgaatc agtatgaccg gagatagcaa aggagaagtg
     7441 gctgatggca gaacaattta cgccagaaat catgacggaa ttgagaaaag atatcgtaag
     7501 agtaccggaa atcattttag aagcaagcgg gatcaagata tttggaaaat tgatccgttc
     7561 gatcatcttt actacggata ttgcgatcat acgtaataca aatgcgaatg cagtaattgc
     7621 tgtgtatcct tttacacctc atccagcgat tactaaaagc attattgagg cagctgacat
     7681 tccagttttc tccggtgtag gtggtggttt gacacaaggt gctcgttctt cctacatgag
     7741 catgttcgca gaagcgcaag gttcgttagg ggtcgttttg aacggtccta cgcctttaga
     7801 gacagtaaaa gaagtttgtc gagtagttga tattccagtt atcagtacgg taacgtcgaa
     7861 atatacagaa atagatgaaa agttagcagc tggtatttcg attatcaata tcagtgcagg
     7921 taaagaaact gcagaagcag tacgccattt tcgtaaaaaa tatcctgaac ttcctatcat
     7981 tgcgacaggt ggtccaacgg aagaaagtat tgctgagacc attcaagcag gagcaaatgc
     8041 catcacatat acgccaccaa gtagcggaga gctttttagt cgtaaaatgg aaaaatatcg
     8101 ggaaactgag aaaaagaatc atgaataaca ggtgagaatc aaaagacgag gaagagcaga
     8161 tagtggctct tcctcgtctt ttgtactcca gtactatttg ttcagaattc aaaaaaaaga
     8221 gaaatttttt tgttttttta cctgtaatgt acataagaag agttaaaacc ttttgttatt
     8281 attagcattc taacaaaaaa atgtcttgat ttattgaagc gctttctata gaatgaaaaa
     8341 cgaataaaaa ctaaaaaagg cacccgacaa tttagtagca gctaaattgt cggggcttga
     8401 aaagtcagcg ggaactgacc aatcaagttg ccatagtcaa tgcttgtgca tcgacgtgta
     8461 tattgtacct aatttcatac agaattacta gttatttttc tgttttttag ggaagagcgc
     8521 acacgaagaa gcatgcttat ttagctatgt caaggatatt gggcagttgt ctgatatcct
     8581 tttttctttg cgtaattatc ctcccatcat tgaagcaaga agtgttttta gcgatgaaag
     8641 aagaatcagc actatctaat ttttcttgaa agtattgagt tgttcatcct aggcagaacg
     8701 ataaggcaag tcttggtttt tattcccctt gaaaaagatc gtttaataaa tatgttctgt
     8761 ataaaaatac agtatattga aaaataacaa tggaagtaga ggtgcagaga atgaagaaaa
     8821 gaatgagaat catcttagct ttaagtgtta tgttattctt cttgacggct tgtgggcaag
     8881 aagatgacaa aaaaatcacg ctgaacatgg cttggtgggg ttcgcaagtc cgacacgatg
     8941 caacagtaaa agtaatcgag ctttatgaag aaaaaaatcc ccatgtgacg atcgattatg
     9001 aattttatga ttatgaagga tattacacaa aattaggtac actagcagca tcagatgata
     9061 tctgggacat tttccaactg aacaatcaat ttcctcaata catcaatcag attgaaccat
     9121 tagatgaata tgtggagcaa gggataattg atatttctaa tgcagatgaa tcctatttag
     9181 caacgacgac ttacgacggg caattagttg gactgtctaa cggggtgaat tcgtttgcga
     9241 ttgcttacaa taaaaatata tttgaacaat taaatatccc tgaaccagct tcaaattgga
     9301 catgggatga atttgaacaa atcagctatc aaatcactga agaattagga atctatgcta
     9361 tttcaagatt cgaagatttt atagctggct gtattattca aataccacaa gttgagaaag
     9421 gattgaattt ttataatcgg gaagatctat ctaacagctt gggatttgaa aatccagact
     9481 atctaacaga ttttttccag ctgcgtaaaa atctggtaga caaaggggcc catccagaac
     9541 caggtggtgg agctgcaagt acgacggatc tgtcttttca ggcggttagg gatagagaag
     9601 cggcaatggt gtttctatca agtaaccagt tgacagaaat attgtcaggg gctcctgaag
     9661 atatggagat tcagttgatt aatccacctc gcagaaaagc agatggagaa tctggtatcc
     9721 ctttacgttc ttcgcaaatg ctgagtatgg caaaaagctc agcccataaa gaagaagctg
     9781 ctaagtttat tgatttcttt caaaacagtg aagaagccaa tgaaatttta aaaggagaac
     9841 gtggtgtacc aatcatgtcg aatattcgag aacagctcgc tcgtgcagat gatgcaactt
     9901 tgcaagcgac atttgattat ttagaaatga tttccgatat ggacaacggc gaagtgaatg
     9961 tctttgatag tgctttgaat ccggaaattc aagaccaata caatctttta gttgaaaaag
    10021 tcatttatga agaagtaaca gcagaagaag cagcagagag tctttatgag tttgcgtcaa
    10081 acattctagg atagcagcag gaggaaggca tgaaaaaaga aaaactaaaa cggttcttgg
    10141 atcaagacaa tgtggtcggt tacgtatttg cttctccatt catcctagga tttctattga
    10201 taacagtgat accgatgatc ttgtcacttt attattcgtt caccgattat cgtttgggaa
    10261 atccagtctc atggatcggc ttggataatt atataaagat gctgagtgat gcccgttttt
    10321 tgaactccat ccgagtcact tttctctatg tatttgcatc tgtacctgtg aaactgatct
    10381 ttggtctatt gattgcttat cttttaacga ggaatatcaa aggaacgact ttctttcgtt
    10441 ctttgtatta tgtgcctagt ttgatcggtg gcagtatttc tatcgctcta gtttggcggg
    10501 agttgttttc taaaaacggg ttagtgaata ttttcctgga acgattaggc gcacctgcgc
    10561 tttcctggtt tagcgatcaa aaactggtca tgatccctct tgttttgatg tctgcttggc
    10621 aattcggttc ggcaatgatc atctttgctg caggtatcaa agagatccca ggctcgtatt
    10681 atgaagcggc agagatcgat ggagcgaaca aatggcagac gttttacaag atcacgttac
    10741 ctcttctttc gccgatcatt ttgtataact tggttatgca gacaatcgcg gcgtttatga
    10801 cttttacgca ggcatttatc attacccaag gtggtccgaa tgatgccacg aacttttatg
    10861 ctctttatgt gtataaccaa gctttttctt ttaattctat ggggtatgct agtgctattt
    10921 cgtggatcat gctgttgatt atgggagctg tcacctttct gattttcaaa tcatcaaaaa
    10981 aatgggtcgt tgtgtgaatg agaggaggaa gaaatgaata atacagtaac gaaatggttt
    11041 caatacggtt ttattttgct attaggtcta gtcatgattt atccggtctt atggatgatc
    11101 gcaggttctt ttaaaacgga tcaagagatt atgagtggtt cgctgaattt gattccagag
    11161 acttttcaat gggaaaatta cgcaaaagga tgggcggggt ttgctggaat ttcatttttt
    11221 actttcttta ggaattcgtt tgtcatctct gcagtttcga cgatagggac agttctgagc
    11281 tcgacttgta ttgcttacgc attatcgaga atcgatttca aaggaaaaag attctggttc
    11341 gtcgtcatgt tagtaacgat gatgattcct gcacaagtga ttttgattcc gcagtttatt
    11401 atttacaatc ggctgaattt ggttgggacg tatgtgccgc tgattttacc acattttttt
    11461 ggacaagcat ttttcattta ccagatcatg caattcatgg tcagcatccc caaagagtta
    11521 gatgaatcag cgattattga cggatgcagc aagtatagtg tctttaccaa aattattttt
    11581 cctttgctga aaccttcgat catcacgacg atcatcatcc agttttattg gaaatgggac
    11641 gacttcatgg gaccattgat ttatttaaac aagccgagat cttacacggt atcgattgcc
    11701 atcaaattat ttgcagacgc tggatctagt acgacttacg cgagtatgtt tgcgatgtct
    11761 acgttgtcgc tgctaccggt ttttctgatt ttcttattct tcaataaata tcttgtccag
    11821 gggatcagta caagcggtct gaagggctga atatgaaaaa gccaatagaa gagatgtttc
    11881 tttacaagct caactactat gtctgtcgga tttttttaac cagtcttatc agtaatgctc
    11941 ttttcgcttt agttctagga tgtttgttca ctttaggatt agtgctgtct gctcgtgttg
    12001 tctatctgat ttattttttg atgatgtatc cttcttatta tctatctgtc cattacttgg
    12061 cagaaaaaaa ggtatgttgg aggaaaggaa aaatcaaacg gatgtgtttc atcattacgt
    12121 tgtttcagct gtttgtcatt atttttgcag ttgattggta tttattagtg ggaaagattg
    12181 gagcttggta tctgattcct tttctattgt gtctgttgct ttttgtatcc catagttttt
    12241 ttagtacgct aattgatatc tgcaaagaag ataaagcaga tagtcttgtt tatttgatca
    12301 aaaggaatat ctggctgttg tcaaaaaacg tcaaggggtt tctgtggtat ttatgccttt
    12361 tgtatggact gttctggtgt atacagcatg attttttact cactgcatta ctgtatccag
    12421 ggctgccatt tgtcacagta ttttgcttaa aagaaaagga gaatgacttt aaatgaagaa
    12481 aaaatatgta caagtcggac ttggtgggag agcgcgtttt ttttatgagg caattgcctc
    12541 tacgtatcat caaacttgtg aactagtcgg tttttgtgat gtatctcaaa caaggatgga
    12601 ttatgcaaat cggatacttg tgaacaagta tggacagaaa aaagtaaaga catgtatgcc
    12661 atcacaattt gagcaactac tggatgacgt acggccggct gaagtcatta ttacgtcagt
    12721 agatcgaacg catcatgatt atatcattcg tgcaatgaaa aaaggatatg atgttatttg
    12781 cgaaaaacct ctgactatcg atgaggaaaa agctcaagaa ataataacgg tccaaaaaca
    12841 gacgggaaga agcctgcgag tcacttttaa ttatcgttat gcaccgtatc acacgaagat
    12901 aaaggaactg atagaaagtg atgtgattgg tgatgtgttt tctatccact tcgaatggct
    12961 gttaaatacg caacacgggg cagattattt cagaagatgg catcgaaata agcacaatag
    13021 cggcgggctt cttgttcata aatctaccca tcattttgat ctagtgaatt tttggttgaa
    13081 cagccgtccc caaaaggtat ttgcattcgg tggattaaac ttttatggga tgcataatgc
    13141 ggagaaacgc ggagtagaaa tattttacag tcgatgctat ggaagcgaac tagctaaagc
    13201 tgatccattt gctatagacc tgtcagccaa tgaggaattg cgagaaatgt atttagaagc
    13261 agaaaaagac agtggatata ttcgtgaccg cagtgtattt tccgatgata tctctattga
    13321 agatacacta ggcgtaacgg tcaaatatga aactggtgcg attttgacgt attcgctaaa
    13381 cgcgtatatg ccgtgggaag gattcaatgt ttctttcaat ggcagcaaag gacggatcga
    13441 aatgaaagtg atcgaaaagt cttacatcaa tgcaggtgga gataaaaagg atgaaggagc
    13501 agccgttctt cagtccatcc atgtctatcc gatgttcggt gagccatatg aggtaatcgt
    13561 cgaacaagaa gcgggcggac atggcggagg agatccgcgt ttattagatg atctatttgg
    13621 tgaagaaaaa gaagaagatc cgtggaatcg ggcagcaact cacgtagatg gtattttgtc
    13681 gattttgaca ggaatcgcag caaatcattc tattgcttca ggaaaagcag tagtgattga
    13741 cgagcttgtc agcttaaaag aataagaata gatttacgga aattacttcc cgtagtttat
    13801 aaaatcttgc cttattgccg ataggctgtc ttttgaacac tatttattcg gaaaaagggg
    13861 ctatgacatg ctttttgtca tgccttcttt ttatgtgggc tgctttttta gataggagaa
    13921 aataaaaata actatttaaa cccgaacaaa aaggaataat gcattaaaaa tcattagatt
    13981 ttacaaaaaa ataagtaaac cacgttgtca gtttcagaaa gtagtgttag actgttttca
    14041 atatttgaaa caacattttt tattcaaata tggcaaactg ccttcacgtg caaaacagtt
    14101 gcaagaaaat gaatttacac tattattagt tttacatacc tacttatata tcgtcataat
    14161 atggatgaca gtttctagcc agtaccgtaa atgctggact ataagtaaaa gattggctat
    14221 ttcacccttc ttttcttatg ataaagtgga gggtgttttt ctttaacaga aacatagtag
    14281 gtttttgcac aaaagcggtt atttcggtat taataaatga taaaggagca aataaagtga
    14341 aagaattagt tgaacgtatt caaaaagacg gacgtatatt aggcgagggc gtattaaaag
    14401 tcgacagctt cgtcactcac caagtagacc ctgtattgat ggaacaaatg ggaaaacggt
    14461 ttgcagaagt atttaaagaa caaggaatca cgaaagttgt gaccattgaa gcttctggga
    14521 tcgcaccagc tttatttgct gcaagagaat tagatgttcc aatgatcttc gctagaaaag
    14581 caaaaagttt gacgatggat gaagaattat tgacagcttc tgtttattct tttacgaaac
    14641 aagtgacaag tacgatttct atttctcgta aatttttatc ttcagaagac aaagtattga
    14701 ttatcgacga tttcttggca aacggacaag cggcaaaagg attgatcgaa ttatgccagc
    14761 aagctggagc gcaagtagaa ggaatcggga tcgtcatcga aaagtctttc caagatggac
    14821 gtcagttact tgaagaaatg ggattgagag tcgtttctct tgcgcgtatt gcttccttga
    14881 cgaatggaca agttgagttt cttgaggagg acgcataatc gtggaaaaag aaacacaaaa
    14941 tggaaaagct gcggttttag ggctgcagca tctgctagcg atgtatgcag gagcagtagc
    15001 ggtgcctctt ttgatcggta caggactagg ctttaatcaa gaacagatga cttatttgat
    15061 ttcaattgat atctttatgt gtggattagc gacattattg caattgacag ttaaccgatt
    15121 tttcggaatt ggcttgcctg tcgttttagg atgtgcgatc caagcagtag ctccactgat
    15181 cttgatcgga accaatgaag gagtaggagc tatctacgga tctattatcg cttctgggat
    15241 ctttgttgtg ttagtttctg gtgtcttttc taaaattaaa aaactattcc ctcctatagt
    15301 aacaggaacg gttatcaccg ttattggttt gactttgatt cctgtagcaa tcgagaaaat
    15361 gggtggaggt aatgcaacag cagctgattt tggtgacaaa acaaatctgc tacttgcatt
    15421 tgtgacgatt ctcctaattg taggcgtgca aatgctggca aaagggttca ttcgttcaat
    15481 cgcggtattg attggtttag tcggaggaag catcttagct gctttcatgg gaatgattga
    15541 tctgagcgcg attggagatg cgccgttgtt ccatgttcca caaccctttt attttggtaa
    15601 accaactttc gacgtatggt cgattttact aatgatcatc atttccatgg tcagcatggt
    15661 agaatcaaca ggtgtatatt ttgcacttgg tgacattact gggaaaaatg ttggagaaga
    15721 agaattgaaa aagggatatc gtgcagaagg acttgccgtt atcttaggcg gtattttcaa
    15781 tacattccct tatactggct tttctcaaaa tgtcggattg gttcaacttt cgggaatcaa
    15841 aacaagaaaa cccatctatt tctctgcatt cttcttgatt gttttaggat tattccctaa
    15901 aatcggtgcg ttagcacaaa tcatccctga gccggtactt ggtggaggaa tgttagtcat
    15961 gttcgggatg gtggcagtcc aaggaatccg tatgctgttg gaagttgatt tcagcaatga
    16021 taaaaatcta ttgattgttg ctgtttctgt tggatttggg ctgggtttca atgttacgcc
    16081 gacattattc agtcaaatgc cgcaaacatt gcaaatgttc actggaaatg gcatcgtaat
    16141 gagtagcatt acggctatca tattgaattt gatctttaat ggattgaaaa aagatgaaga
    16201 aatattggaa gaagccttca agaagtaata gagcaaaaga aaggttgtga aatcagcgtt
    16261 ttgacctgat tgttagagaa acaaacggaa aaatagttcc tcgtattttg ccgtttgttt
    16321 gagctaatac cgagggtcag ctgattgaac accgttgaag gaagaggtcg tgagcctgcc
    16381 gttcatctcc gagtcacaag gaacaatttc aaaaaaaagc ttctcatgtt tgttgaatat
    16441 tgttccttgt gaccgaggag ttggcaggag aacaccgttt atccataaaa aggaatgtga
    16501 cctaactttt gtcacattcc ttttttttat ctttaataaa cacgaacaat aaaatgtttt
    16561 tctttgttaa cattcgaaat ttctttgaca tcaaaaaaag tgattgttaa aatatgaatg
    16621 tagaaaaact gggaggaaaa acaatgacag caaaaatctc tgtgattatg gggagtactt
    16681 cagattggga aacaatgaaa gaagcatgtc aaatattgga tgaattcggt gtgtcttatg
    16741 aaaaaaaggt cgtttctgct catcgaaccc cagatttgat gtttgaatat gcacaaaacg
    16801 ctcgaaaaaa aggaatcaaa atcattattg caggagcagg gggagccgcg catctgccgg
    16861 gtatggtcgc atcaaaaaca actttgccgg ttatcggtgt accggtccaa tcacgaacat
    16921 taaatggttt ggattcgctt ctttcgattg tccaaatgcc tggaggagta cctgtagcta
    16981 ctacagcaat cggaaaagcg ggagcaatta atgctggact acttgcgata cagatgcttt
    17041 ccatgtatga tttagaattg gaggctaaat tggctgaaag acgttcaatg ttggctaaaa
    17101 ctgttataga aagtagtgat caacttggtt aaacctttgt tgcctggttc aacgatcggg
    17161 atcgtcggtg gcggtcaatt aggtcgtatg atggcgatta gtgcaaaaga aatgggcttt
    17221 aaagtaggtg tgctggatcc tgtgagcgat tgtccagccg cacaagttgc agattggcac
    17281 attgaaagtg tctatgatga cacctttgcg ctgaaagagc ttgccagaag aacagatgtc
    17341 attacctatg aattcgaaaa cgtcagtgta gaggcgttaa atgcgatttt acccatgtca
    17401 tttattcctc aagggacaga cttgcttgcg attacgcaag accgcctgtt agaaaaatct
    17461 tttttggaaa cgaataacat cgtgattgct ccttatgcaa cgatcgtcag tccgacggat
    17521 atccaagatg cgattgacgg gatcggctat ccttgtgttt tgaaaaccac tcgaggcggc
    17581 tatgatggca aggggcagta tgttttgaaa agtcgggcgg atctagctcc tgccatggac
    17641 cttttaagag aaggaacctg tgttttagaa gcatggatcc cgtttgaaaa agagatttcg
    17701 atcatggtgg caggaaatgg tcaaggagat ttcacgactt ttccggtcgt ggaaaacatc
    17761 caccacaaca atattcttca tgaaacgatt gctccagccg cgattgatca ggatgtgatc
    17821 gaggaggctg agcgtattgc ccgtgtgatt gcagaagcag tgagcctttc aggtgtttta
    17881 ggggtagaaa tgtttttaac aaaaacaggc gggctatatg tgaatgaatt agctcctcgt
    17941 cctcacaatt ctggacatta ttctattgaa gcttgttcta tgagtcagtt cgatgcccat
    18001 attcgaggta tttgtggttg gccattagga gaaaaagtac gtttgataag caatgcagta
    18061 atggtcaatg ttttgggaga acaactgctg gaaagctatc gcttgattga gaataagcca
    18121 gactggcaat tccatttcta tggaaaaaaa caagcaaaaa aaggtcgcaa gatgggacat
    18181 atcactatct tgacagaaga catccagcaa acgctggaag aaatcaaaca aacgaatatt
    18241 tgggattaga ggagaaaaaa catgttagaa cgttatacac gccctgaaat ggggaatatc
    18301 tggacggaag agaacaaata ccgtgcgtgg ctcgaagtcg agattttagc agatgaagct
    18361 tgggcagaac ttggtgaaat cccgcaagaa gatgtgaaaa aaattcgtga gcatgcatca
    18421 tttgatatcg atcggatctt ggaaatcgaa aaattaacac gccatgatgt cgttgcgttt
    18481 acacgagcgg tttctgaaac attgggagaa gaaagaaaat gggtccatta tggtctaaca
    18541 agtacggatg tcgtagatac tgcttatggc tatctcatca agcaagcaaa tgatatttta
    18601 cgggaagact tgaaacgttt tacggaaatc attggcgaaa aagcaaaaga acacaaatat
    18661 acagtgatga tgggtcggac acacggtgtc catgctgaac caactacttt tggattgaaa
    18721 cttgccacat ggtattcaga aatgaaacgg aacatcgaac gatttgaaca tgcggcaaaa
    18781 ggagtagaag caggaaaaat ctcaggagca gtgggaacgt tcgcaaatat cccgccattt
    18841 attgaagagt atgtatgtaa acaattgggg atccgtccac aagaaatctc tactcaagtt
    18901 ctccctcggg atctacatgc tgaatatttc tcagcgatgg cgcttattgc tacaagtatt
    18961 gaacgttttg caacagagat acgcggattg caaaaatccg aaacacgtga agtagaagaa
    19021 ttttttgcga aaggtcaaaa aggttcttct gctatgccgc acaaaaggaa tccgatcggt
    19081 tcagaaaata tggctggtct agccagagtg gtgcgcggac acatggtcac tgcctatgaa
    19141 aatgtcacat tatggcatga aagagatatt tctcattctt cagcagaacg aatcatcttg
    19201 cctgatacca cgattttatt gaattacatg ttaaatcgtt ttggcaatat cgttaaaaat
    19261 ctaacggtct ttcctgaaaa catgaagcgg aatatgtctg caacatttgg tttgatttat
    19321 agccagcgcg ttatgctgaa attgatcgat aagggaatga caagagaaga agcttatgat
    19381 cttgttcagc caaaaactgc gtacgcttgg gacaaccaag tgcagttccg cccgctgtta
    19441 gaagaagatc caaccatcac atcggtatta tctaaagaag aaatagacga tgcctttgat
    19501 tatcatcatc atttgaaaaa cgttgacgtg atattcaaac gagtaggttt ggaataaaga
    19561 agagtgagtt gcaaaattca attaaaatag aatagcaaaa ataaccaaaa aagaagcaga
    19621 tccgttagcg gttctgcttc ttttagttta ttatattttc ttatcaataa taggaacctg
    19681 attttaaaag cggctacctc tttaccgtgc ctctttacat acaaaaaata gaggaattaa
    19741 gcgtaaatgg cttggctatt ataactaaaa tgctggttat ggagatgttg cagcaagttt
    19801 tcgagaaaag cgtattccat ctcttgtcca ttgaataaga tctggatacg ataatgattt
    19861 ttgaaacgct tctgttcatg aatctgactg attgaaagtt cgcctttgtg ttctgtctga
    19921 taaagaaaag tccttttacg gcattcagca cagcgcttgg ttgctcggcg gatttttcgg
    19981 aatgtataaa aactagtatc tttattgcat aaataaacgt ctttgatcga cagcaaatca
    20041 ttatatagac aggaacgttc ttcagctgtt aattctgata aaaaaagctg accttgtttt
    20101 attgcatctt ccatcggcaa gtgcctcctc gaacccatta tccaaatttt tagttaattc
    20161 taatatacca tcattaagta taaatgtaaa cgcttgttga tggtataaga ttttcgtttc
    20221 tgataaaaca cgaacattaa aagaatctaa tgccttaaat gtacatattt ttgttgattt
    20281 ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct
    20341 tttaatgtaa ataaaaagga gagacgtaca tggagaagaa agaacttttg tatgaaggaa
    20401 aagccaaaaa aatatatctt acttctgatg agaacttgat ttggatagaa tatttagatc
    20461 aagcaactgc gttgaatggc gcgaggaaag accagataag tggaaaagga gaattgaata
    20521 atcaaatcac aagtgcaatt ttccgttatc tgactcaaag aggaataaaa aatcatttta
    20581 ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt acaaattatc cctctggaag
    20641 tggttatccg aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta
    20701 aagaattcat caaaccaatc attgaattct actataagga tgaccagttg gatgatccat
    20761 ttatcaatga agagcatatc tatttcttac aacttgcaaa gccggaagaa atcagtgagc
    20821 tgaaagccca agcgttggct attaatgaaa ttttgaccga attatttgct aaggtcggtt
    20881 tggttttagt agatttcaag ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg
    20941 atgaagtaac accggatact tgccgattat gggatgaacg tacgaatgaa catatggaca
    21001 aagacgttta ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa gtatataacc
    21061 gtctatcgga actaactgaa gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt
    21121 aacctataaa gattcagtgt tagatcctca aggggaggca gtaaaaaaag cagtccatcg
    21181 tatgggatat gaaacaatcg aagatatccg aataggaaaa tactttgaga tacaacttgc
    21241 tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa
    21301 tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg gaggaagtat aacatgaaat
    21361 ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg
    21421 agatcatggg cgctgatgcc gaatttgtac ggcatgatga gtcctcttta gaaggatttg
    21481 atggggtttt actgccggga ggattctcct atggtgacta tttgagatgc ggtgcgattg
    21541 ctcggttttc tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat
    21601 tcggtacatg caatggattc cagatattga cagaagcagg acttttgcca ggtgtactga
    21661 gacgaaatga gtctttgcat tttatttgca aaactgtacc attgaaagtc aaccccaaga
    21721 cggcctttac ttcagagtat gaagccgacg aaataatcta tcttccagtt gctcatgggg
    21781 aaggaaatta tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat aatcagattg
    21841 cctttactta taatgacaac atcaacggca gcgtggaaaa cattgcaggg attacgaaca
    21901 aagcagggaa tgtgttagga atgatgcctc atccagaacg tgcagtagaa gacttgctag
    21961 gatcagaaga tgggaaacgg ttctttgctt ccattttaaa aaacttcgga aaggtgaccg
    22021 tatagatgaa aacattgaaa gaaccgacag ctgaagagat caagaaaaaa agaatctatg
    22081 ccgaatgggg attgactgat gaagaatatc gaatgatcga agaagatatc ttaggaagaa
    22141 tgcctaatta tacggaaact ggactgtttt ctgttatgtg gagcgaacat tgttcctata
    22201 aaaattctaa accggttcta cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc
    22261 ctggagaagg cgcaggcatc gtcgacatcg gtgatggact agcagttgtc ttcaaagcag
    22321 aaagtcacaa tcatccttca gccgtggagc cttatgaagg agctgcgaca ggagttggcg
    22381 gaatcatcag agatattttc agcatggggg ctcgtccaat tgcgttgcta gattcattga
    22441 ggttcggcga attagataat ccaagaacga aatatttact agaagaagta gttgcaggta
    22501 tcggcggata cggcaattgt atcggcatac cgactgtagg cggcgaaatt gcatttgatc
    22561 cttgttatga aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac
    22621 atatccagaa agggcaagcc gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa
    22681 caggtcgcga cggtatccac ggagcaactt ttgcctcaga agaatttaat caagaagaag
    22741 aacagcaacg ttctgctgta caagtaggcg atccttttat ggaaaagctg ctgcttgaag
    22801 cgtgtttgga attaatcttg catcattcgg attctctaat aggtatacag gatatgggag
    22861 cagcaggact tgtttcatct agtgcggaaa tggcttcaaa agctggctca ggacttatat
    22921 tgacattaga tgaagtccca caaagagaaa ctgggatgac accttatgaa atgatgttat
    22981 cggaatcaca agaacggatg ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag
    23041 aactatttca gaagtatgat ctagatgcag tcactatcgg taaagtaaca gacgacgggc
    23101 aatatcggct atatcatcaa ggagaagaag tagcaaatct gccagtcgat gcattagctg
    23161 aagatgcacc agtttatcat aaagaaatga aagaaccagc tcgaatcgta gaatttcagc
    23221 agttagctcc ttatcagcca gttatcgaag aacctggaca agtattgctt gatctgctgc
    23281 agcaaccgac gattgcttca aaacgttcaa tctacgaaac ttatgattca caagtacaaa
    23341 ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga gggacgaata
    23401 aagcgttagc tatgaccaca gactgcaacg cgcgctatct gtatttggat ccaaaaatcg
    23461 gaggacaaat agcagtggca gaagctgcta gaaatattat tgctagcggt ggtaagccct
    23521 tggcgatcac cgactgcctg aactatggtt ctcctgataa accagaagtt ttttgggaat
    23581 tatcgacttc agctgacgga attgctgctg cgtgtgaaac gttaggaact cctgttattt
    23641 caggaaatgt ctctttatat aatgaaacgg atggtcaagc aatctatcca acaccaatga
    23701 ttggtatggt cggattgatt gaggatcata aacatatcac tactcaggaa ttcaaaaaat
    23761 caggggatct aatctatata ttaggtaaga cgtttgcaga ttttgatggc agtgaattac
    23821 aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa
    23881 agagaaacca agagttagta ttgacagcca tccaaaacgg attgattgaa agtgctcatg
    23941 actgttcaga aggtggcttg gcaattgcac tggcagaatc agctttcaaa catcagctag
    24001 ggatatctgt acagtttgaa ttgtcctctg ctcaattatt tgcagaaact caatcaagat
    24061 ttgtgttgac agtcgcaccg gaaaataaga cacgattcga agaaatgatg ggagatgctg
    24121 ctgttttagc aggtaaggtg acggatgaag cgataatcga aatttcagca actgacggtc
    24181 agatcaaaat agaaactgca gtagcaagaa agtgctggga ggatgcaatc gtatgtctta
    24241 tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt gtttggggtc atccagacgc
    24301 cgctagtgtg gcatacttcg ggctccatag tcttcagcat cgaggacaag aaggtgcagg
    24361 tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga gatttggggc tgctttctga
    24421 ggtattcaaa gatcaaagaa aattgcagca gttaacagga gaagctgcaa tcggacatgt
    24481 acgttatgca acagcaggca atggaagtgt ggacaatatt cagccttttc tattcaaatt
    24541 tcatgatcag tcgatcggct tagcgcataa tggtaacttg atcaatgctc gttcccttag
    24601 aaaagagtta gaggaaaatg gggcgatctt ccattctaac tccgatacgg agatattgat
    24661 gcatttgatc cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa
    24721 tgaagtcaaa ggtggctttg cctatttatt aatgacagaa acggcaatga ttgccgcttt
    24781 ggacccaaat ggttttcgtc cattagcaat cggacaaatg aaaaacggag catacgtgat
    24841 tgcctcggaa acttgtgcat tagaagttat tggggcacgt tttataagag atgtccagcc
    24901 tggtgagata gtgatcatca atgacgaagg aatccagatt gattgcttta cgaaggacac
    24961 gcagctttct atttgttcga tggaatatat ctattttgcc agaccagatt caaacatcgc
    25021 agggatcaat gtgcatactg cacggaaaaa tatgggacgg aatttagcga aagaatcgcc
    25081 agtaaaagca gatatggtcg ttggcgtacc taattcttct ttgtcagccg caagcggtta
    25141 tgcggaagct agtggaatcc cttatgaaat cggattagta aaaaatcaat acattgcccg
    25201 aacatttatc cagccaacgc ctgagctaag agagcaaggt gttcgaatga aattatctgc
    25261 tgtacgagga gtagtggaag gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg
    25321 tacgacaagc agacggatcg tccatctttt gaaagaagca ggggcaaaag aagtccatgt
    25381 gaggattgct tctcctcctt tgaaatatcc atgtttttac gggattgata ttcaaacgcg
    25441 taaagaactc attgcggcaa atcacacgat tgatgaaatc aaagaatgta tcggtgcaga
    25501 ttctttaggt tttttaagtg aagagggtct gatcgaatcg atcggtctgg aaaaagatgc
    25561 tccgtattca ggattgtgta tggcttattt caacggagat tatccgacgc cgttatatga
    25621 ctatgaagaa aaatatcagg aatcactaaa agaaaaagta tcgttctttt aaaagcagac
    25681 caagaaggga gtatatgaat tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg
    25741 aagctggata tgaagtagta gaacggatca agaagcatgt caaaaaaacg gaacgtttag
    25801 gtatgatggg cgctatagga ggattcggcg gttgttttga tttatcggtc tttcaattaa
    25861 aagagccagt ccttgtctcg gggaccgatg gcgtggggac gaaattagca gtggcaatcg
    25921 aagcagacaa acacgataca atcgggatcg actgcgtagc gatgtgtgtc aatgatatct
    25981 tagcacaagg cgcagagccg ttatatttct tagactactt agcaacgggg aaaaatgatc
    26041 cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg
    26101 cgctgattgg cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat gattatgatc
    26161 tagccggatt tgctgtaggg atagctgaaa aatctcaatt attgacagga gaaacgatca
    26221 acccaggaga tgtgttgctt ggaatagcat ctagcgggat tcattcaaat gggtactctc
    26281 ttgttcgcaa aattttcttt gagcagcagc agttttcttt atcatcaaaa ctgccagaat
    26341 tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa aatctatgtt tcttccttgc
    26401 ttccgctgat caaagaaaag aaaataaaag gagctgcaca tatcactggt ggagggtttg
    26461 tagaaaatat cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa cttggctcat
    26521 ggccgattct tccaatattc ggctgtttac aaaaatatgg tgaaattcct gagatggaga
    26581 tgtatgaaat cttcaatatg ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa
    26641 aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga
    26701 tcgccaaaga aaaacatcgt gtggtgttta taggagaaga acgatgagaa tcgctgtttt
    26761 tgcttctgga aacggaagca actttcaagc gctagccgat tatctatcga aaaaaggatt
    26821 ggaatcttcg attgactggt tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc
    26881 aactgcgttg agtgtaccgg cagattgttt ttcacctaaa gaatttgatt caaaaaaaga
    26941 gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag atcgatttga tcgtattagc
    27001 tggatatatg cgaatcattg gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa
    27061 tatccatccg tctcttttac ctgccttccc tggtttgcat ggcatacgtg atgcctttga
    27121 agctggagta aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg tagatactgg
    27181 accaatcatc cggcaggaaa aagtgagaat tgaacaggag gacacgttcg attctttaga
    27241 agaaaaaatc catagagtag agcatcgaat ctatccagaa gtgattagtg aaattattga
    27301 aaatggaggc tatctgaaat gacaagagca ttgatcagtg tatcagataa aactgggatt
    27361 gtagcattcg cccaaggttt gattgaacaa ggagtagaaa tcatttcaac aggaggaaca
    27421 aagcatgcgt tggataaagc tgggatcaaa acgctttcga tcgaagaagt gacagggttt
    27481 cctgaaatga tggatggccg tgtcaaaaca cttcatccta aaatccatgg tggtctttta
    27541 ggaagacgcg atctccctag ccatatggag gcaatggaaa cggaaaatat ccaaccaatc
    27601 gactttgttt gtgtcaattt atatcctttc aaagaaacga ttatgaaacc agatgtcgaa
    27661 atagcagaag ctattgaaaa cattgatatc ggtggtccta gtatgctccg tagtgcagca
    27721 aaaaaccatg aatttgtcac ctctgttgtt gatccatctg actatgaaga agtactttca
    27781 gaattacgtc agtatgggca tacttcactt tctttcagac aaaaactagc ggcaaaagtc
    27841 tttcgtcata ctgcggcata tgatgcactg atcgctcaat acttaacgga ttgggtggaa
    27901 gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa aacaaacgct tcgttacggt
    27961 gaaaatagcc atcaagccgc tgccttttat cagtccgctc ttctggaaaa atattcgatc
    28021 gcagcagcaa aacaactcca tgggaaagaa ctttcttata ataatatccg agatgcagat
    28081 gctgcgctac ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt gaaacacatg
    28141 aatccttgtg gtattgggac agcatctacg attttagaag cgtgggaagc agcgtatgaa
    28201 gcagatcctg tttcaatctt tggtgggatc atcgttttga atcgagaagt agatctgcca
    28261 acagcagaac agatgcataa gctgtttcta gaaatcatca ttgccccttc ttatacacaa
    28321 gaagctttgg aattactgca aaaaaagaaa aacatccgat tactgacagt agactttgcg
    28381 caggaagaaa atcaaaatcg agaagaaacg gtctcagtct taggtggact tcttgtacaa
    28441 gatcaagatc tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg
    28501 actccagcag aagcaaaagc attagcgttt gcttggagag ctgtcaaaca tgtgaaatct
    28561 aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa tcggagccgg gcagatgaat
    28621 cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt
    28681 ttagctagcg atgcttattt tccaatggat gattcggttg aatatgcagc caaacatggt
    28741 ataaaagcaa tcattcagcc aggaggaagc atcaaagacc aagattctat tgatatggcg
    28801 aataaatacg atctagcaat ggtctttact gatgttcggc attttagaca ttagagagga
    28861 gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa
    28921 aaacttttag ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa tccggggatg
    28981 acaaaagatg gtatcaattg tgtcgctatc agtgagcgag atcattcagc gttaatccat
    29041 tttgttaaga aagaggctat tgattggagt atcgttggtc ctgaagttcc tttgttaaat
    29101 gggatcgtcg atgattttat ggcggaagga ttgaaaatct tcggtccgac aaaagcagca
    29161 gctctgatcg aaggttcgaa aagttttgct aaacaattaa tggatactta tcaaatcccg
    29221 acagctaaat ccagaagttt tgaatgttac gaaaaagcag cagcgtatat caaagaaaat
    29281 ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt agtcgtggct
    29341 accacagtga aggaagcgct ggatgcggca gaacaaatgt tgaaccagca tcgatttggg
    29401 gatagtagca aaaaaatcgt cgtagaagag tttttggcag gagaagaatt ctcccttttg
    29461 gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt cgcaagatca taagcgggca
    29521 tttgataacg ataaagggcc aaatacaggc ggaatgggtg cttattcacc agtaccgcaa
    29581 atttcaaaaa agatgattga aaaagctgtg gaagaagttt taaaaccagc ggctttggga
    29641 atgaaagaac taggacgctc tttcacgggt gttttatatg cgggtttgat tgctacgaag
    29701 gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt
    29761 ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca
    29821 gtagagttaa agtggcagca agaaggatac aatgtaggcg ttgtggttgc agctgccggt
    29881 taccccgaag aatatgagac aggaatggtt ctaccagatt ttctggaaga agagctttct
    29941 gtttactatg ctggagtttc ctcaaataac ggcaaactag ttggagcagg aggacgtctt
    30001 tatttagtag aagcatacgg tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg
    30061 ttaatggata aaacgacaga tggaacattt tatcgtacag atattggaga aaaagctgcc
    30121 aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat acaacaaaca
    30181 taatcggaag cgccgatttt agtgaagcct tttcataaag agtgtggtac acttttgttg
    30241 tgtatataaa taaggaggag ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa
    30301 agcagctgca gtgatcttag gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt
    30361 gattgagaca gcttatgaaa acggcattga tttttttgac catgcagata tttatggaaa
    30421 cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa acatcaatca aacgagaaga
    30481 tctttttatt caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa
    30541 acatatcatt gaatctgtag aaaaaagttt gacccgtctg aaaatggatt atgtggatgc
    30601 tttacttctt catcgtccag atacattggt agaaccagag gaagtagctg aagcgtttga
    30661 tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca
    30721 aatcgaattg ctgaaaaaat atgttcgtca accattagta gctaatcagc tgcaatttgg
    30781 cttgaaacat actggaatga tcgatcaagg gattcatgtc aatatgacag atgaagcaag
    30841 tatcgatcat gacggtagtg tacttgatta ttcccgattg catgatatga cgatccaagc
    30901 atggtcacca taccagtatg gattttttga aggtgtattt atcgggaacg aaaaattccc
    30961 tgaattaaac caaacattag aagaactagc agctaaatat caaacgacac caactggctt
    31021 ggcttctgct tggattttac gccatccagc caatatgcaa gtgatcgccg gtacgatgaa
    31081 cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc gggaagattg
    31141 gtacgaattg taccgtgctg ctggaaacat tttaccttaa agattgtcat aagcaggtca
    31201 aaagggctat aaattaaaaa gacagaataa cttgaaagct gttttcagct tcgagatatt
    31261 ctgtcttttt ttattgcaaa tggaaagagt atcattgtac ggatttcgtt tattagttat
    31321 ttttgttttt aaggaaaagc tgtgtccgat attcttttgg agaaatccct tgttttttct
    31381 taaataaata actgaaatag ttagggtcat tataaccaat ttcaaaagca atatctgaaa
    31441 tacgatagtc tgattgctgc aataattttt ttgctaattc gattctttgt tctgtcagat
    31501 aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt actgaaatga gagggactta
    31561 atgcaacttg ttgagcgacc caagttaagg acatatctgg atcggtaaaa ttttctttga
    31621 taaaatccaa tgcatggttc aaaattggct gatatttgtt ccttgaaggc tgtgagggca
    31681 gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc
    31741 tgattgctaa gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg attttctttt
    31801 ctttcaggca gacatgctgg agtagcttag tcaagacaaa taaacgatat aatcgtgttc
    31861 gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta cttagatact tcgtttttcg
    31921 ataatgaaga caaatcaagt gagaggtcaa agtaaaagac agaatcaact ggaagtttcg
    31981 cttcttgaat caaatcctca tagctgataa tttgttcttt aagggaactg gcggtttcaa
    32041 gtatcatctg cgtgatctca aaagatgcag gtaattcgct gattctttct gcgacaggac
    32101 caatggcgac agaaaaatta tttttttgtc ccttttctaa ttcatggacc aacgtttggg
    32161 ctacctgata actggtctcg atcacatttt cttcaagaga atcaactatt aaaaatttga
    32221 tgaatcgaga ggaaacagca gagaagatca cttgttgatt ctccctaaat aaaaacttca
    32281 aataatcact gagatgagcg taatcttcaa agtctgtact gacttggttt gctgctaata
    32341 atacggtcat ttttttccct gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt
    32401 cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa atgatttttt ttcaattcga
    32461 aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa atctaaagca gttactgctt
    32521 tttctattgc ttgtttcaat tcgctacttt taatcggttt aagcagataa tcatctgcct
    32581 gtacctggat tgctcctttc atataatcga aatcgtcgaa tccactaata aatatgagac
    32641 gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca
    32701 tttggatatc agtcagaatg atgtcgggct gtaagtcgat aattgaagac agtgccatct
    32761 caccatctcc agcttcgccg acataagtga ttggataata agaaccaagg acagttattt
    32821 gttcccgcaa gttattgcga atgaggtatt cgtcttcgat gataaaaact ttatacatgt
    32881 ttgtttcctc caattgtttt gggagataga gggatcatca gtgtgacagt tgttccttct
    32941 cggtatttgc tgtcaatcgt gataggtttt tgatcatttc cgtaataaag aaggagacgc
    33001 ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc tttggctaac
    33061 tctttacgta tttctgccag tttttctgca gggatgccaa tcccgttgtc tataactttg
    33121 aattggatca tctcatcaat tttgcatgtg gtaatacgaa taaatccttt gtgacggacg
    33181 tacttcgttc catggtatac agcattttca atcaatggct gcaagatcat ttttaaaata
    33241 gaaacaggaa aaagtgtttc tggtatatcg atcttatagt caagcatatg gccgtatctt
    33301 atttttaaga tgactagata atcattgata tggctaattt ctttttcaag aggaatccag
    33361 tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga
    33421 gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg catccaaact gttataaata
    33481 aaatggggag taatctgagc ttgcaataca cgaacttcac tttgtgctaa atggtattgt
    33541 ttcaaagcgt tctcccgtaa agactggttt agatcttccg ccattttatt catgctagcg
    33601 gtaagtgttt gtaattccag tgtttccggc agggttaaac gaaagccaag gttccctgaa
    33661 gaaagttgtt ctgacatctc gatcagctga ttgatcggtt cttggacctt tctctctaaa
    33721 aagcgacgat tctttttcgc atagaaaaaa atggtactaa gaactccaag ttccactatt
    33781 gttaatagaa tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc
    33841 acacgaacaa aatcttgtaa gatatctgac agcagttgag tgacagaatc tacctgattg
    33901 atgatctctt cattggtatc cataaaatct tttgccaaga tattattgat cagctcgttt
    33961 tgataatctt ccagtgtatc gatcgtacgt agcgatacat ctaacgtact aatttcttta
    34021 agtgtatttg tattattttt gatctctcga atatcattcc taagttcttc taggatattt
    34081 tcctcattga actcatctac ggttacttgg ccagaaatca agtcccacat actttccaaa
    34141 actttttgat caaccttcaa agcaattgca tctgcttttt ggatatttgc gatcgtctgt
    34201 tgataaatca aaagctgttg cgaatataac agtatgctca gtagcaaagg gataacagca
    34261 aatagaagca tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa attttttgtc
    34321 atgatacttc tccagtcaat aatttctggt tgggatgtta gctatattct gctgggaaaa
    34381 gacagtctcg gaaataaaaa tatctttagg taatgttttt cctgtttgaa tatttccaaa
    34441 ataacgagta acggtattgg ctacatacca gccgacatca ggtttacact cgactacaca
    34501 attgacgatt cctgagcgta gcttctcgat catatcttct tgtccatcaa tagttacaat
    34561 gatcaaatct tctccaggtt tgattgatgt ttcttttatc gcagctaaag caccgtgagt
    34621 catttcatca ctatggctgt ataatacatc gatttcggat aaatcatttg tttctaaata
    34681 tcgtttcatc tggtcttttc ctttcatccg aataaaatca ccgacaattc ggtgaacaat
    34741 agtcatcgga ggtttatggt cgatggttcc atcttttttt agggttggtt tacgattgat
    34801 tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg gagttctctg aaccagcaag
    34861 ttctaggatt ctgatttcct gtttctttga atttgtgaag tgattgctga caaataaacc
    34921 cgcccgattt ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc
    34981 attcgtttga atatgtctgt cgacaagtat gacagggatc cctgctcgct ttgcttcttt
    35041 taatacagag tcccacccgg attcttgcaa aggagtaaaa atgatcatat ccactttata
    35101 ggcaatgaac gtccgtatat cttggatttg ccgttcctga ttcatgtagc catttcggta
    35161 gagaacttgg taatcttctt tttcaagcgc agtgataatt gattcagtat gctttttccg
    35221 ccagctactc tccgttcctg attgcgaaaa accaacgact agtgggtcag gtgcttcctt
    35281 tgtattaggt gtacacccca tcatttggta agcgattaca acaagggcta aaaaaaataa
    35341 aacacgtttc actttaaatc actccatctg gatttgttag tactatctta tcagataaaa
    35401 gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca
    35461 agacaaaaaa agtacaccta aaaaatttaa agtaaatccc ataaaaaaat caatgttaac
    35521 gctttcttta ttagctatac tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac
    35581 aaatttagaa agattgttgg gactatcgct attttgaccg caagcattct attaagtgcg
    35641 tgtggcaatt caggatctgc agatgatagt acggggtatg taggaatcgc tatgccgacc
    35701 aaatcagcag aaagatggat cgctgatggg aacaacatgg tctcggaatt agaaaaactg
    35761 ggttataaaa cagatcttca atatggagaa gataaagtag aaaatcaagt agcacaaata
    35821 gaaaatatga ttacaaaagg ggtagataca cttgtgattg cttctatcga tggttcggct
    35881 ttaactgatg tgttagctaa agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt
    35941 ttactgatga attcagaaaa tgtcgattat tacgcgactt ttgataattt tggagtaggt
    36001 gtatcgcaag cagcttatat tgaagagcac ttaggattga aagaaggaaa aggaccattc
    36061 acgattgaat tatttggtgg ctcgcctgac gacaacaacg cactaatcaa ttacaacgga
    36121 gtgatgtctg ttttacaacc atatatggac aacggtcagt tggtcgtgcc atctggacag
    36181 acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca agcaaggatg
    36241 gataatctgt taagcgcaaa ttatactgac caaacattgg atgctgtttt gtcgccatat
    36301 gatccaataa gtttaggaat catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt
    36361 aaacctttac ctgtgattac tggacaagat gcgacagttg ctggcgttaa atcgatcatt
    36421 gccggggaac aaacacaaac gatctttaaa gacacacgta tcttagcaaa aaatacgatt
    36481 gaaatgatca aagcaatcag tgatggagaa gaggttccgg tcaatgatac tgaaacttat
    36541 gataatgggg tgaaaactgt ccctacgtat ttagcaaata cagtatctgt cgataaagat
    36601 aattatcaag cagagctgat cgacaccgat tattataaag aatccgatct gaaaaattaa
    36661 gaggaaccaa acaaggaact aaagagatct aagtcagaga gaggataaga attatggcgg
    36721 attgtatttt aaaaatggca gatatcacca aaaaattttc tggaatccga gcgttagatt
    36781 gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg
    36841 gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc atatggctcc tatgacgggg
    36901 aaatctttta tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag
    36961 gcatcgtcat cattcatcaa gaattagcac taagtcctta tctttcaata aaagagaata
    37021 tttttctcgg aaatgaacaa gcagaaagag gcgtgattga ttgggaactg acagaaaaga
    37081 aaacatatga actgctgaat aaagtcggtt tgtctatcga cccgaatctt ctagtatccc
    37141 agattggcgt agggcaacag caactagttg aaatagctaa agcattatca aaatccgtcc
    37201 gtctgttgat tttggatgag cctaccgctg cattgaatga agaagaaagc gcgaatttgc
    37261 tgagattgat ccgagagttg aaagagcaag gtgtaacctc catcattatt tcacataagc
    37321 tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt acgggatgga cagacgattg
    37381 agacactaga aaatgaaatg atcagcgaag agcatattat tcgcggaatg gtgggcagag
    37441 atttagttga tcgttatcct gaaagaaaac cggatattgg agaagtttat tttaaagtag
    37501 aagactggac ggtctatcac cctcttgatc acgagcgaat cataaacgac cacatcaact
    37561 tgcatttgaa aaaaggagag attgtaggaa tagctggact gatgggagcg ggcagaactg
    37621 aattcgctat gagtctattt ggacattctt atggaagcaa gatatccgga cgtatattaa
    37681 aggaaggggc agagatatca gttaagaccg ttccttcagc cattgcaaac ggactcgctt
    37741 atgtctcgga ggaccgaaaa tctttaggac tgaatctgct gatggatatt cgggaaaacg
    37801 tgacgctgag cagtttggat aaaataagtc aaaaaggagt attagataaa gaaaaagaag
    37861 taactgaggc agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa
    37921 ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt tttatccaag tggctgatga
    37981 cacaacccga tattcttttt ttggatgagc caacccgtgg gatagatgtt ggggcaaaat
    38041 atgagatcta cacgattatc gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct
    38101 cttctgaact tcctgaaata ttagggatgt gtgatcgaat ctatacgatg aacgaaggac
    38161 gtattacagg agaagtctcg cgagaagatg cagatcagga agtcttgatg aaattaatga
    38221 cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc ttacggataa aaaagagaaa
    38281 aaactaaatt tgaaaagtag agcagctgat ctttttggtc gctacagcat ggtgatcatt
    38341 ttagcgggac tacttattgc gtttcaattg atgacggatg gtattttctg gcgtccattg
    38401 aatataacta accttgttct gcaaaatagc catatattag tactagctgc agggatgtta
    38461 ttagttgtgc tcttaggtca tgtggatttg tcggtagggt cagtgatggc gtttgttggt
    38521 gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac agttatcgca
    38581 tgtcttgcag taggcgggct gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg
    38641 ataccggcgt ttatcgtgac tttagcagga ttgctgatgt ttcgtggact gacacaagtt
    38701 gttctaggag gccaatctct agcacctttt ccagctgttt tccaaaaaat gtctacgggt
    38761 tatttaccag acttaagcga aggaagcagc cttcacttct tgacattgat tttaggaatg
    38821 atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat
    38881 ttatttgaag tggaatctcc aacagctttc ttggtaaagg cggtatttac agcagtcgtt
    38941 gtactaggaa taacttatat ctttgcctct tatcaagggt atccgattat tttgatcatt
    39001 cttggtttga tcgttgcagc atatgggttt ttgacaaata aaacagtagc aggtcgacag
    39061 atttatgcga cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa aacaaagcga
    39121 atcacgttct gggtattcgt caacatggga gtcatggcag ctttagctgg attagtttta
    39181 gctgctcgat tgaatgcggc aacacctcaa gcaggtacat cgatggaact ggatgcaatg
    39241 gcggcagtat attttggtgg tgcttcgact tccggcggca ttggaacaat catgggtgcg
    39301 attgtcggtg gcttggtcat gggtgtattg aacaatggca tgtcgatttt aggcgtcggg
    39361 gtagactggc agcaggcaat caaaggattg atcttgcttt tagccgttgt gctagacttg
    39421 tataataaga aaaaacgaac aacgtaggag aacgccggtt attcagaaaa aagactgtga
    39481 caagacattt gtcacagtct ttttttcgct aaaagcgaac attcattgtt tcgtttgtat
    39541 agactaaaaa taatatatgt ttttttacca ttcgtataaa ttctcttttt gctcttgatt
    39601 tttttgctta attgagttaa attacatagg gcgctaaaaa aagccttgat taaattaata
    39661 atgttcgtgt tttgctggat gtgaacatca ggaggataat gtaatgaaag tatttgatta
    39721 cgaagatatt caattaattc ctaataaatg tatcgtaaat agccgttctg aatgtgatac
    39781 aacggtcaca ttaggtaaac atacattcaa aatgccagta gtgcctgcca acatgcagac
    39841 gattattgat gaaacaatcg cagaattctt agcggaaaat ggttatttct atatcatgca
    39901 tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat
    39961 tacctcgatc agtgttggcg tcaagaaaga ggaatattcc tttatagaga agttggcaga
    40021 agaatcgcta aatcctgatt atatcactat cgacatcgcg catggacatg ctaactcagt
    40081 tattgatatg attcagcata ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa
    40141 tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt
    40201 cgggattggt ccaggaaaag tatgtattac aaaaatcaag acaggctttg ggactggagg
    40261 ctggcaactg gcagctctta gatggtgtgc gaaagcagct cgcaagccga tcatcgctga
    40321 cggtgggatc agaacacatg gcgatattgc aaaatctgtc cgttttggtg cgacaatggt
    40381 catgattggt tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa
    40441 tggaatcgtg tataaagaat actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg
    40501 aaatgttgaa ggaaagaaaa tctggatcca acacaaagga tctttgaaag atacactagt
    40561 ggaaatgcaa caagaccttc aatcatctat ttcttatgct ggaggaagag atctggaagc
    40621 cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt tttaatggag acacaatcta
    40681 aaaaataaat aaaatattag gatgttgtga aaaagttgtc ctgcattaag taataagaac
    40741 agccaaacaa aaatagttct ttatatgttt tgtttggctg gaattattat cgcagataca
    40801 agcttttgaa catcattatt cagaataagg gactgtgata agatttttgt cacagtccct
    40861 tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa
    40921 atagacaata gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt
    40981 tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa acggaaggat caatcctgga
    41041 agtcaacttg aatcagaacg aaaactaggg caactttttg gtgtgaatcg ttccaccgtt
    41101 gtccatgcat tggaagagct tagggagctt ggggtactaa cgagcaaacg tggcagcgga
    41161 tggtttgtta atcagactga atgggggaaa ttcgctgttc caagaatcga ttggcggcaa
    41221 atgatctctc ctcgttatga gcagacggac tggtatgagc aaaaaataag agaagcaaag
    41281 cagatccaaa aaacatcgtt tcttgattta tacgctagcg agatgccaga tgagcttttg
    41341 ccgaattttg agtttccttc ttattctctt gaagaaatct tagcggaaga aaaagaaatg
    41401 gatcttttag gctatactcc gttacgtaac aaaatcaaac ggtatttgga aagtaaattt
    41461 gaattggcat tttcccccaa ccagcttctt gtgactggtg gcggtcagca agcaatcttt
    41521 ttgattttac aaacgattct ttcagctgga gaggggatag ctgtagagtc accttctttt
    41581 ttttaccgat tacctctttt caaagcttca ggaacgcgtt tatttgggat accgatggat
    41641 gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa attgaaagca
    41701 gtgcttatca acccgaattt tcaaaaccct acaggaaaag tgatgagtca aaaacgacga
    41761 gaaaagctag tagaattgtg tcgacagtat cggcttccaa ttattgaaga cgatgtattt
    41821 acggatcttt catttctaaa ccaagaagaa gtccttccta tacgaacgat tgatccggat
    41881 aatgtgttat acgtcggttc gttatctagg gttttaggaa aaacaacgaa aatcggttgg
    41941 gtgataggac ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat gatggagttt
    42001 tcgatgaata tccttacaca aatcacagtg gctaatgtat ttaatgaagc aattgatttg
    42061 aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag cgcttgggga
    42121 gattcacaat cgtttttcaa gcttcattca attttaggtg gttactatgc ctggattact
    42181 tgggatggga aaaaactaga ccccgaacta gcagaaaaag tagtagatat gggtcttgga
    42241 attgcaccaa gtctactttt tgggcaagat ataaatggaa tcagaatcaa ttacagtagg
    42301 ctggatgaaa accaaatacc gttgttttat tcaaagatga aaattctaga aaagtggtta
    42361 acagaataat ttcacaaagt aaatagatta aagaatataa cagcttggat ttaaatttca
    42421 agctgtttct tttttttcgg aaaaagaata cgatataaag aaactaagaa aaaaccatta
    42481 tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca
    42541 ccaacgtttt taaatagaaa cagatggttg taagtttgat tagaaaatag aaaaaatgca
    42601 gatgaaatat aaaacgcttt ccttattgtg attttattca ctaatgctgt taatatgcgg
    42661 attggcataa tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat
    42721 ccatgataat ttattaatga agacggttaa atagtgatac agttttattc actgtttcaa
    42781 tcgcagaatg aagagatttt cttatagatg agagaggaat gtgtgacaaa tggcaaacaa
    42841 aaaagcaccg atcgacttcc aagcactgct agatgaagtc aatgcagatt tcccagtata
    42901 tcaagcactt gatcaagatg gaaacgtagt taacaaagat ttagtacctg atttatccga
    42961 tgatgaactc gttgaattaa tgactcatat ggtatggtct cgtgtattag accaacgttc
    43021 aacagcgctg aaccgccaag gacgcctagg cttttttgca ccaactgctg gacaagaggc
    43081 aagtcagctg gctagtgctt acgcatttga taaagaagat gttcttcttc caggttatcg
    43141 ggatgtacct caactagtaa aacatggttt acctttatct caagcttttt tatggtctcg
    43201 aggacatgct gcaggtaatt cttatccgga agaattgaaa gctctaccac cacaaattat
    43261 catcggtgca caatatgtac aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa
    43321 gaaaaatgtc gtcttcactt atactggtga tggcggttca tcacaaggtg atttttacga
    43381 agcaatcaat ttcgctggcg cttaccatgc aaatgccgta ttttatatcc aaaacaacgg
    43441 gtatgcgatc tctacacctc gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc
    43501 tgtcgcagct gggattcctg gtattcaagt cgatggaatg gatccgcttg cagtatatac
    43561 tgtatctaaa atggcacggg aatgggcgat cagtggaaat ggaccagtcc taatcgaaac
    43621 actgacttat cgttatggac cacatacact atctggtgac gatccaaccc gctaccgtac
    43681 aaaagacaca gatgacgatt ggcagaaaaa agatccattg atccgcttcc gtaaatattt
    43741 gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga
    43801 agaaatcaaa gcagctattg ctgaagccga caaagtacca aaacaaaagg tttcagattt
    43861 cttgaaaaac atgttcgaag tatctccaca atcaatcaaa gaacaaattg caatttatga
    43921 agcgaaggag tcgaaataat catggcacaa aaaacaatga tccaagcaat cacagatgcc
    43981 ttagcacttg aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa
    44041 aacggaggag ttttccgggc aactgaagga ttgcaggaaa agttcggaga agaccgagta
    44101 ttcgatactc ctttagctga atctggaatc gcaggattga gtttcggtct tgcgttagaa
    44161 ggatttcgtc cagttcctga aatccaattc tttggtttta ttttcgaagc aatggacgaa
    44221 gtagtagctc aaatggctcg tacaagatat cgaatgagcg gcactagaaa tctacccatc
    44281 acaattcgtt cgccatttgg tggcggtgtt catacaccag aattgcattc ggataattta
    44341 gaaggtttga tcgctcagtc tcctggtatt cgagtagtga ttccttccaa cccttatgat
    44401 gcaaaaggac tattgatttc ttctattaga agtaacgatc cggttgtttt cctagaacac
    44461 atgaaactat atcgctcatt ccgagaagaa gtgccggatg aagcttacga agttcctttg
    44521 gataaagcag cagttactcg tgagggaaca gatgtatcta ttatcacata cggagcaatg
    44581 gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag aaaatatatc tgttgaaatc
    44641 attgatcttc gtacagttgc gccattagat gtagaaacga tcattcaatc cgtggaaaaa
    44701 actggtcgcg tagttgttgt ccaagaagca caacgacaag cgggcgttgc cgcgcaagta
    44761 gtttctgaga tctcagaacg cgctatcctt tcattagaag ccccaatcgg acgagtttct
    44821 gcacctgata cagtcttccc atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa
    44881 gatatcgaag acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa
    44941 gtcatggctt atcaatttaa attgccggat atcggtgaag gaatcgcaga aggtgaaatc
    45001 gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag atgatacatt actagaagtt
    45061 caaaatgaca aatcagtaga agaaatcccg tcacctgtta caggaacggt taaaaatgtg
    45121 attgttccag aaggtaccgt agcaaatgtt ggggatgtat tagtagaaat cgatgcacca
    45181 ggacacgaag acaatgaagg ggacagtggt gtagccgcag aatcccaaac tccagctaaa
    45241 ccggctgctg aacccactgt tgatacagaa tcagctggat cgtcaagcga aggagtcttc
    45301 caatttaagt taccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc
    45361 gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa
    45421 tcagtagaag aaattccgtc acctgtcaca gggacagtta aaaacgtgat tgttccagaa
    45481 ggcactgtag caaatgttgg ggatgtatta gtagaaatcg atgctcctgg acacaatagt
    45541 gcaccgtcaa ctagtgcgcc atcagctgaa gcaccaaaag aaaaagtgga aacatcaggt
    45601 tcagcaagtg tcgttgaagc tgccgatcca aataaacgtg tattggctat gccatctgtt
    45661 cgtcaatttg cccgtgaaaa agatgtagat atctcacaag taactgcaac tggaaaaggt
    45721 ggcagagtaa caaaagagga catcgaaaac ttcttggcag gtggtccaag cagtgcacca
    45781 gcgaaatcag aagcgccaga agcggcagca ccgaaagaag cagctcctgc agcagaaagc
    45841 aaaccagctg caccagcaaa accattcaag tccaacttag gagacttgga agaacgtgta
    45901 gcaatgacgc caacacgtaa agctatcgct aaagcaatgg tcaacagtaa gcatacagct
    45961 ccacacgtga cattgcatga tgaagtagaa gtgtctaaac tatgggataa tcggaaacga
    46021 tttaaagaag ttgctgctgc aaatggtacg aaacttactt tcttgccata tgtcgtgaaa
    46081 gctttaacag cgactgttaa gaaataccca gtattgaatg catctatcga cgatgcaaac
    46141 caagaaatcg tttataaaca ttactacaat atcggtattg caactgatac ggatcacgga
    46201 ctatatgtac caaatgtgaa agatgcagac cgcaaaggaa tgtttgctat tgcagacgaa
    46261 atcaatgaga aagcaaaatt agctcatgac ggcaaactat ctgcagaaga tatgcgcaat
    46321 ggaacaatta cgatcagcaa tatcggatct gtaggtggcg gttggtttac accagtcatc
    46381 aactatccag aagtagctat tttaggtgta ggaacgattg cacaacaacc aatcgtaaat
    46441 gctgaaggcg aaatcgttgt cggtcgtgtg atgaaactgt cattaagttt cgatcaccgt
    46501 atcgtagatg gagcaactgc gcaacaagca atgaataata tcaaacgtct tttagctgat
    46561 ccagagctat taatgatgga aggatgataa acaaatggta gttggagatt ttgctgtaga
    46621 attagataca gttgtaattg gtgcgggacc tggtggctat gtagctgcca tccgcgctgc
    46681 tgaaatggga caaaaagtag caatcattga aagagagtat attggcggtg tttgtttgaa
    46741 tgtcggctgt attccttcaa aagcactgat agctgcagga caccattatc aagagtcact
    46801 agattctgaa atgtttggag taactagtga aaacgtcaaa ctagattttg caaaaacaca
    46861 agaatggaaa gaaaacaaag ttgtccatac gttgacttct ggtgttggtt tcctattaaa
    46921 aaaacataag gttgaaacaa tcgaaggaga agcgttcttc gtagatgatc acacactacg
    46981 cgtcattcat ccagattcag ctcaaactta ttcgttcaat aacgcaatca tcgcaacagg
    47041 ttcccgtcca atcgagattc caggatttaa attcggagga cgtgtgttag attctactgg
    47101 cggattagca ttgaaagaag tacctaagaa atttgtgatc atcggtggcg gtgtgattgg
    47161 tgccgaattg ggcggagcgt acgcaaactt aggagcagaa gtaacgattc ttgaaggctc
    47221 accacaaatt ttaccaactt acgaaaaaga tctagtaaaa ttagtagaag atgatttcaa
    47281 gaaaaaaggc gtaacagtcg taacgaatgc aatggcgaaa gaagcagtcg ataacggtga
    47341 cagcgtgaca gtgaaatatg cagtggacgg gaaagaagaa tccgttactg ctgattatgt
    47401 gatggtcact gtaggtcgtc gtccgaatac agatgatatg ggcttagaac aagcgggtgt
    47461 cgaagtagga gaacgaggat tgatcacggt ggataaacaa ggacgtacga acgtacctaa
    47521 catctatgcc atcggtgata tcgttccagg ggcggcatta gcacacaaag ccagttatga
    47581 agcaaaaatc gctgctgaag caatctctgg caaaaaagta gctgtagatt ataaagcaat
    47641 gccggcagta gccttcactg atcctgaatt ggcttctgta ggtatgacga tcaaagaagc
    47701 aaaagatgct ggtatcgaag caaaagcata taaattccca ttttcaggga acggtcgggc
    47761 attatcgctt ggtaaaacag aaggattcat ccgtctagtt acgacgatcg aagataacgt
    47821 gcttattggc gcgcaaatcg gtggagtcgg cgcaagtgat atggtctctg aattagcttt
    47881 agcaatcgaa tcaggaatga atgctgaaga tatcgccttg acgattcatc ctcatccatc
    47941 tcttggcgaa atcgtcatgg atgcttcgga attagcatta ggtttaccaa tccatatttg
    48001 atttgaataa tacaggcgaa acgcaactga aaaatcagtt gcgttttgtt ttttagaatt
    48061 atcaataata ttattatgta aacttaaaaa tatgacaact tcttggttta ttaccacttc
    48121 ctatgctaca ctgaagagga atatgaaata ggagagttga agatgcaaga atatcagtat
    48181 ccacttgatt tagattggac aacagacgaa atggtcattg taatgaagat gtgggaagat
    48241 ctggaacgtg cgaatgaaaa agggataaac aatgaagctt ttttgaagac ttatcagcag
    48301 tttaaaactg ttgtcaaatc cattggtgag gaaaaacgat tgggacggga atttgaaaaa
    48361 gcttccggtt attctttata tcgtacagtg cagcaagcaa aaaaaaatcc aaacaaaact
    48421 ttgagaatga acgggtgaga taaatggaac agatgattga tcatatcaag tcctggcttg
    48481 aagaagctgg tgagaaaatc aaatcttctc aacaaacatt aacaatctcg caaaaatcaa
    48541 atcgaacgga tctagtaaca aatgtcgatc gaaacatcca gaattttcta atcgaaaaaa
    48601 tccattcctt tgatccagag gcgaaaatat tggcagaaga agaagggtgt aatcaattat
    48661 cggatctcga tggaagagtc tttatcatcg atcctattga tggcaccttg aattttgtga
    48721 ttgaagggga aaatttttgt attatgctcg ctgtttatga atccggcgtt ggaaagcttg
    48781 gtttcattta tgacgtgatg agaaatgaac ttttttgggg tggaaaagga ataggcgtgt
    48841 ataaaaatga tgagccattg cctagaccag caaataaatc cttatcggaa ggcttacttg
    48901 gtgtgaatag ttatcttttc ggtcataata aatatggaat ccgagaaatt gcggaaagca
    48961 gcatggggac acgtatgtac ggatgcgctg gtcttgaact agtggcaatc ttgaaaggaa
    49021 accacattgc ttatatttca aaccttagcc cttgggatta tgcagcggga aatgtcttat
    49081 tggaagagtt tggtatggta acaagcggtc tttttggtac tcctttgaca tttgaaaaaa
    49141 gggaatacta tttagcaggg acttcttgtg catatgcaga aatcgcagac cttttgaaaa
    49201 aagaaggctg aaaaaaaggt agaaatcatt cacaaaaatg ataaaaacga gcaaaagatt
    49261 aattttctcg aaaaaatgaa aacattttac tatatttcat gaaaaaagtc tgctataatt
    49321 agcagtcgag taaaatttgc cctgtcataa agaaccaata aaaaaggagt tagttacatt
    49381 gaattataga aacgatatcc gtaacgtcgc aattattgcc cacgttgacc acggaaaaac
    49441 aacattagtt gatgaactat taaaacaatc agatacatta gacgcacata cgcaattgca
    49501 agaacgtgcg atggactcaa atgcgctaga aaaagaacgt ggtatcacga ttttagccaa
    49561 aaacacagct gtagactata aaggtattcg cgtaaacatc atggatactc caggacacgc
    49621 ggacttcggt ggcgaagtag aacggatcat gaaaatggtt gacggcgttg tgttggtcgt
    49681 agatgcgtat gaaggaacaa tgcctcaaac acgttttgta ttgaaaaaag cactggaaca
    49741 acatatcaca ccaatcgttg tcgtaaacaa gattgacaaa ccttctgctc gtcctgagca
    49801 tgtcgtagat gaagttcttg aactattcat cgaattagga gcagatgatg atcagttaga
    49861 ctttcctgtg atttatgcat ctgcactaaa tggtacgtca agcttgtcag atgatccagc
    49921 agatcaagaa ccaaccatgg cacctatttt tgatacaatt attgaaaaaa tccctgctcc
    49981 tgtcgataac agcgacgaac cattacaatt ccaagtttca ttacttgact acaacgatta
    50041 tgtaggtcgt atcggaatcg gacgtgtatt tagaggaaca atcaaagtag gagaccaagt
    50101 agcattaatt aaattagacg gaacagttaa gaaattccgt gtaacgaaat tatttggttt
    50161 ctttggacta aaacgtctag agattcaaga agcaaaagct ggagacttga ttgctgtctc
    50221 aggtatggaa gatatctttg tcggtgaaac agttactcct gtggatcatc aagatgcgtt
    50281 acctatcctt cacattgacg aaccaacatt gcaaatgact ttcttagtta ataattcacc
    50341 atttgccggt cgagaaggta aattcgtaac cgctcgtaag attgaagaac gtttgatggc
    50401 agaacttcaa acagatgtat ccttacgagt tgaaccaaca aactctccag atgcatggac
    50461 agtttctggt cgtggagaat tgcatttgtc tatcttgatt gaaaacatgc gccgtgaggg
    50521 atacgaattg caagtatctc gtccagaagt tattgaaaaa gaaatcgacg gtgtaaaatg
    50581 cgaaccgttt gaacgcgtgc aaatcgatac accagaagaa tatatgggta gtgttatcga
    50641 gtccttgagc ttacgtaaag gtgaaatgca agatatggtc catacaggaa acggacaaat
    50701 ccgtttgaca ttcttaacac ctgcccgcgg tttgattggt tattcaactg aattcctttc
    50761 aatgacacgt ggttacggaa tcatgaacca tacttttgac caatatctgc caatgcttcc
    50821 tggtcaaatt ggcggacgtc accaaggggc gcttgtttcg atcgatacag gaaaagcaac
    50881 gacttattct atcatgagta tcgaagaacg cggtacagtc ttcgtagaac caggtacaga
    50941 agtatatgaa ggaatgatta tcggggaaaa cagccgtgat aatgatttga ctgtcaacat
    51001 cacgaaagca aaacaaatga caaacgtacg gtctgctact aaagaccaaa catctgtcat
    51061 caaaaaacca aaacaattga cgttggaaga atctttggaa ttcttgaatg atgatgaata
    51121 ttgcgaagta acaccagaaa gcattcgctt gagaaaacaa attttagaga aaaatgctcg
    51181 tgaaaaagca tctaaaaaga aaaaataatc tgtaaaagga tgaagagaga aaccagcttg
    51241 ttataaaaaa tagctggttt ctttttttgt ccaactctt
//
LOCUS       DACDLI010000011        51138 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDLI010000011 DACDLI010000000
VERSION     DACDLI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.011"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(620..3769)
                     /locus_tag="IV353_000700"
     CDS             complement(620..3769)
                     /locus_tag="IV353_000700"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9008972.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3782..4999)
                     /locus_tag="IV353_000701"
     CDS             complement(3782..4999)
                     /locus_tag="IV353_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9008973.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(4989..6584)
                     /locus_tag="IV353_000702"
     CDS             complement(4989..6584)
                     /locus_tag="IV353_000702"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9008974.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(7093..7875)
                     /locus_tag="IV353_000703"
     CDS             complement(7093..7875)
                     /locus_tag="IV353_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008975.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(7940..8776)
                     /locus_tag="IV353_000704"
     CDS             complement(7940..8776)
                     /locus_tag="IV353_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9008976.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8794..10203)
                     /locus_tag="IV353_000705"
     CDS             complement(8794..10203)
                     /locus_tag="IV353_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9008977.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIKNNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10217..12040)
                     /locus_tag="IV353_000706"
     CDS             complement(10217..12040)
                     /locus_tag="IV353_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9008978.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12883..13209)
                     /locus_tag="IV353_000707"
     CDS             complement(12883..13209)
                     /locus_tag="IV353_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008979.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13255..13617)
                     /locus_tag="IV353_000708"
     CDS             complement(13255..13617)
                     /locus_tag="IV353_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9008980.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13654..13869)
                     /locus_tag="IV353_000709"
     CDS             complement(13654..13869)
                     /locus_tag="IV353_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9008981.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            13996..14331
                     /locus_tag="IV353_000710"
     CDS             13996..14331
                     /locus_tag="IV353_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9008982.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14407..16404)
                     /locus_tag="IV353_000711"
     CDS             complement(14407..16404)
                     /locus_tag="IV353_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9008983.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVACILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16502..16777)
                     /locus_tag="IV353_000712"
     CDS             complement(16502..16777)
                     /locus_tag="IV353_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9008984.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17009..17743
                     /locus_tag="IV353_000713"
     CDS             17009..17743
                     /locus_tag="IV353_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9008985.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17782..18924
                     /locus_tag="IV353_000714"
     CDS             17782..18924
                     /locus_tag="IV353_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9008986.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(19019..20410)
                     /gene="sufB"
                     /locus_tag="IV353_000715"
     CDS             complement(19019..20410)
                     /gene="sufB"
                     /locus_tag="IV353_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9008987.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20415..20885)
                     /locus_tag="IV353_000716"
     CDS             complement(20415..20885)
                     /locus_tag="IV353_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9008988.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(20872..22107)
                     /locus_tag="IV353_000717"
     CDS             complement(20872..22107)
                     /locus_tag="IV353_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9008989.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(22104..23390)
                     /gene="sufD"
                     /locus_tag="IV353_000718"
     CDS             complement(22104..23390)
                     /gene="sufD"
                     /locus_tag="IV353_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9008990.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23403..24176)
                     /gene="sufC"
                     /locus_tag="IV353_000719"
     CDS             complement(23403..24176)
                     /gene="sufC"
                     /locus_tag="IV353_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9008991.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(24373..25221)
                     /locus_tag="IV353_000720"
     CDS             complement(24373..25221)
                     /locus_tag="IV353_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9008992.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(25245..25931)
                     /locus_tag="IV353_000721"
     CDS             complement(25245..25931)
                     /locus_tag="IV353_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9008993.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(25924..26958)
                     /locus_tag="IV353_000722"
     CDS             complement(25924..26958)
                     /locus_tag="IV353_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9008994.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(27353..27691)
                     /locus_tag="IV353_000723"
     CDS             complement(27353..27691)
                     /locus_tag="IV353_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9008995.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27693..28049)
                     /locus_tag="IV353_000724"
     CDS             complement(27693..28049)
                     /locus_tag="IV353_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9008996.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(28055..29242)
                     /locus_tag="IV353_000725"
     CDS             complement(28055..29242)
                     /locus_tag="IV353_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9008997.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29433..30278)
                     /locus_tag="IV353_000726"
     CDS             complement(29433..30278)
                     /locus_tag="IV353_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9008998.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30512..31477
                     /locus_tag="IV353_000727"
     CDS             30512..31477
                     /locus_tag="IV353_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9008999.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            31854..32897
                     /locus_tag="IV353_000728"
     CDS             31854..32897
                     /locus_tag="IV353_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9009000.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(32959..33423)
                     /locus_tag="IV353_000729"
     CDS             complement(32959..33423)
                     /locus_tag="IV353_000729"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9009001.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            33669..34616
                     /locus_tag="IV353_000730"
     CDS             33669..34616
                     /locus_tag="IV353_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9009002.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(34827..36899)
                     /locus_tag="IV353_000731"
     CDS             complement(34827..36899)
                     /locus_tag="IV353_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9009003.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(36832..37209)
                     /locus_tag="IV353_000732"
     CDS             complement(36832..37209)
                     /locus_tag="IV353_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9009004.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(37338..37898)
                     /locus_tag="IV353_000733"
     CDS             complement(37338..37898)
                     /locus_tag="IV353_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9009005.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            38048..39340
                     /locus_tag="IV353_000734"
     CDS             38048..39340
                     /locus_tag="IV353_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9009006.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(39400..40518)
                     /locus_tag="IV353_000735"
     CDS             complement(39400..40518)
                     /locus_tag="IV353_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9009007.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(40532..41953)
                     /gene="arcD"
                     /locus_tag="IV353_000736"
     CDS             complement(40532..41953)
                     /gene="arcD"
                     /locus_tag="IV353_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9009008.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(42199..43320)
                     /gene="mnmA"
                     /locus_tag="IV353_000737"
     CDS             complement(42199..43320)
                     /gene="mnmA"
                     /locus_tag="IV353_000737"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9009009.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            43724..44323
                     /locus_tag="IV353_000738"
     CDS             43724..44323
                     /locus_tag="IV353_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9009010.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(44461..45747)
                     /gene="efmA"
                     /locus_tag="IV353_000739"
     CDS             complement(44461..45747)
                     /gene="efmA"
                     /locus_tag="IV353_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9009011.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(46018..46362)
                     /locus_tag="IV353_000740"
     CDS             complement(46018..46362)
                     /locus_tag="IV353_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9009012.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(46499..47635)
                     /locus_tag="IV353_000741"
     CDS             complement(46499..47635)
                     /locus_tag="IV353_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9009013.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(47824..48798)
                     /locus_tag="IV353_000742"
     CDS             complement(47824..48798)
                     /locus_tag="IV353_000742"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9009014.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(48878..49753)
                     /locus_tag="IV353_000743"
     CDS             complement(48878..49753)
                     /locus_tag="IV353_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9009015.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            49883..>51138
                     /locus_tag="IV353_000744"
     CDS             49883..>51138
                     /locus_tag="IV353_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9009016.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTL"
BASE COUNT        14723 a        10716 c         8886 g        16813 t
ORIGIN      
        1 atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga cctgttaaat
       61 ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc aaaacaagcc
      121 cgctgagagt aaaaaatata cagtcccata ggtaaagtgc cacaggactg tatatttttt
      181 tatttgagat tacctgaaat gattccattt aatttttatc tcattttttc ctgctagtta
      241 gtatcaaata ctctcctatt ttctcaataa actattttgg gttacatggt atctgaaaca
      301 gctaaaacct tgtcattata gcaaaaaatc actttatctc cgtttcattt cagatacatt
      361 tcacttaagc ggattccaaa aaaacaaaag aaaaaggaac ccctagttag cctatgcttt
      421 ttattggttc ctctcaaaac acggtcatat aacaacgggg tggggtaaat gaatgaaaaa
      481 ggtggaggaa acattattca atagctgtct taaatacttc gttataaaat gtgaacaaat
      541 aaaagacatc tatccctatt ttctacactt ccacttgact accatctttg aatcgtacaa
      601 ttttcttaac tcttctcgtt tacctcaatt cattctcaag tggaatcaac tcatcatcta
      661 acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggcttc gcttctggat
      721 gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata ttaggaattg
      781 gatcttcacc tggtagatat tgcagattag aagcatgtaa ttctttctca gaaacaaacc
      841 actcattgga aaagacatga atcaaatcat cactcgcttg tgtaaaatag cgacgtttaa
      901 tgacaaagat atcttcatta ctatctaaag tccccacatc aaatgcattt aaaatttgtc
      961 ggtaaacttc tttgactcct tctgttttat tcaatttagc caaagctttt tcaatttctt
     1021 ctcgtgcatc tgaactattc gcagcataag ttcctagtaa ttgatcaata taagttgaat
     1081 caatatcata caacttagtg gtattttcac tataaaattc gatcgttgaa aaatcttctc
     1141 cttctttttc tggttcttcc tctattaatg aacccttcac ggtctcgtac acgccaattt
     1201 gttcttccaa aagttgaatt tgattgttca aagttggaga tttcaccatg tcatcattgt
     1261 aatcgtcata agtcactaac gattcataag cacgattcaa ttcttgaaat gctttgacgt
     1321 actcgatacg gatttcaagt ggcgtatgtt catcaatgga ttccatggat gaaacctctt
     1381 ttaacttacc atatgcttgt ttgaatcttt ttttggattc ctgataactt ggataaatca
     1441 aacctgattc ttcttttgct tttgaataca gttttgtcgc atcctcgaca ttcttctcca
     1501 ttgtcgctgg cttacgaaac gtcacaatca atccttttgt cttatcagga tacgtacgat
     1561 tcgtacgtga aaaagcttgg atcaatcctg catactctag attacgatcc acaaataggg
     1621 tttgaatcgt aggggcgtca aaacctgtta atagacgatc cactacaatc gctaaatcga
     1681 tttgtttccc aaattgtttg aattccgctc tttttcgtgc caaacgatta ttgatgtctc
     1741 cgttataacg ctcaatgtct gctaatgacc aggaagtatc ataatactga ttatattcct
     1801 caataatttt ttgcatttct tcttgttggg cacttgaatc tcggctattt tcttctaatg
     1861 aataggtaat agcgattcgt ggaaaatcag gatcctccat cgttcgtcct tcacgaatga
     1921 gctgattggg aaactctttt gttaaccaat caggctcttt cgtcatctct ttgatggccc
     1981 gataatactt tttggccatg tcaatggaac tcgtggttaa gattgcagat ttcgttggcc
     2041 tgccatttcg aaaatcaaat tttgtatagg cattatctgg acggaacatt ttatgaatga
     2101 ctttttggat atgttcatct gcttcaaaaa cagctgggtc caaataactt tcttttttga
     2161 caggttccat ttgatcaatc atacgattga tctgttctga tgaatactct gcatatgttt
     2221 ctacttcttt taatttccga tagattttat tctctagtga agtcggctcc acggtgtttt
     2281 catgctctac ttgaaatcct aagacagagc catcttctag cgcattctta atggtatagg
     2341 tatgcaagac ctctccatac tgatcatggg ttgtacgtgc caattgccct ttggcttgtt
     2401 tccgattctc aggaaaaatc ggtgtacctg taaagccaaa ccaagtggat ttcggaaaca
     2461 tttttttgat ttcttccata ttttcagcac ttaacgctcg atgacactca tctactacaa
     2521 acacgatatg ttggcccatt aatttttgga agcgattggt tccttttttc tcttcttgtt
     2581 tctttgcata tttcaacgcc gcatctagtt tttgacgggt tgtaatgatt acaacatttg
     2641 cattggcatc tgccaacagg gtttcactta attctttggc acttcccgtt ccaacgatca
     2701 agctattcgc tttggcgttc ccggaagaaa tcccagtatt aaattccgaa gcaaattttg
     2761 taaactctgt ggtggtttga ttatctaaat ctttgcggtc cactaacatg atcgttcgat
     2821 cgatccccga ttttcgtgct aaaagcttag tagatacaaa actagtcaac gtctttcctg
     2881 aaccagtcgc atgccacaca taccctgatt gatgtttatt tgcggcaata aacaaggctt
     2941 gaatcgcatg tacttgataa ggatgtaaca ccatgagggt tttattgtct tgatcttcac
     3001 tgacgattgt ataatccgca atcaaacgat gagcatccgg gatatttaac acttgtttac
     3061 aaaactcata taagttttct actttttcat tgtctttcgt tcgccaacta aataaaaact
     3121 tagggtgcaa gtctttcggt aaggcatttg caaaataccg agtggtttgt tcattggaaa
     3181 ccacaaacaa ctgtaaggtc gaaaagatat tgttccgaaa cataccttct tctgcatatt
     3241 ttttgatttg gttaaatgcc tgataaacac catcttttgc agttacttgt ttcagctcga
     3301 tttggacaat cggtaatcca ttgattaata aggtgacgtc aaaacgtcga tcccgtgctt
     3361 ctatgttact tccacgtttt gcaatttgat ggaccacttc ataagtagag attcctccac
     3421 caatgtcttg attggaataa aggataagtg agacagaacc caattgcgaa tcttcccgtt
     3481 caatcgtgat ccgagccatc ccattttcac cttttaacca tttcgcagca tcaaatgggg
     3541 ttttcgttcg cagcagtaat tccgttttga tcgtttcaaa ttctttatcg gtcaaaggaa
     3601 aatcattcag ttctgcttga ttatttgaga taattttttg gcgtaaattg acccaaaggt
     3661 cttcttctga ttttagatcc ggacgatacg tccactgatt atgcccttct ccaagtacac
     3721 gaatcaactg ttcttcgact tctgcttcct cacggtgact aattttcatt cttcttccct
     3781 cctagacaaa catcttttgt aagaagcttt ttttcgtttc ttttagtaaa tctaacttac
     3841 gttgatgaag agcgatcgta tcatctagct gtttgaagaa agtaccgatt ttttgttgtt
     3901 catcctttac aggaacctta acactgatat tctcaatatc agaactgtta taagaaggca
     3961 atgcccctac ttgggagtat cttggcaaat ttatagtttc aaacaatatt ttaccaaaat
     4021 agatatccca agtattatta aataaataag ccatggtatt gttatctatt aagaaagggc
     4081 ttgtgaccaa acgttttcta ttaagcatta ttgctgcgcc aactttagca aatataatcg
     4141 ctggcacatc ttttattggc ttccattttt tacgctcaat ttgctcatta gatacataat
     4201 gatttgcatt tctcatttca tactcattac caatgttatt catgtcggaa actttaaaaa
     4261 atggagtacc ttcagaacca ccttgctcgg catcaggaaa tccaattccc gattgagttt
     4321 tacctaattc tcccaactta cgctcttccc aatcttccgt aaaaccagga aaccggattt
     4381 ctggcacttt tgctccgttt tcagggaaca ttttttgtaa gaatcctttt ttcgtttcct
     4441 tcagcaaatc taacttacgc tgatgaagag cgatcgtgtc gtcaagttgt ttgaagaagg
     4501 tgccgatttt ttgttgttct tcctcactag gaaacattgc tactatattt ttaaactttt
     4561 ttagagacag aacatgttga cctgttccct ctgcttgaat agataattgt cgttggattc
     4621 cattgttaat acgtcttaag aagaagtcgg aatcagcaga ttttaaagta aatacaggat
     4681 aaaaatagct tataatccct ctatcaataa tgtcattacg gttaaacaca aacacatcat
     4741 tatcacttcg actacggaac gtgaaatatc ctctaggtag tacaaagtaa ccaatattat
     4801 tttctgtagt tacttgccga tttgcaaaat agtcttcttg caaaactatc cctttttgtt
     4861 gagatgaagt tagcatagga aattgatttt gtacacttgt cttttcaaca taatctacaa
     4921 cgaagtcccc caacttcctc tgttcccaat catctgtaaa tcctgggaaa cggatttctg
     4981 gttgtgtatc attgctcatg tttgaacacc tccagcgcac cttgtaacca cgcttcattt
     5041 tcaggtgttg tttgcaggga agagatagct tctaaaagtt ttttttctag cacttgctgt
     5101 tcttgtcgta tcttcttgat gtcatttccg acatgaacca tatcaattgg ttcttcttct
     5161 tcgaaagtat ctacatagcg tgggatattt aagttatagt cattttcttt gatttcatca
     5221 aaggttgcca aatgagcgta tttctcgaca tcttttcttt cagcgtaggt ttcgaggatt
     5281 ttttgaatgt tttcttccga tagtttattt tgatttttcc ctttaacaaa ttcacgacta
     5341 gcatcaatga ataatacatc tcgagtttga cgattcttct tcaacacaat caccgtcgtt
     5401 ggaatcgatg tcccaaagaa caagttcgca ggcataccga tcacagcata aatactgcca
     5461 tcttctagta atttttgacg gatgacccct tctgcagcgc cacggaataa aactccgtgt
     5521 ggtaagacga tggccatggt acctgtttcc ttcaaatgat agaacccatg aagaagaaac
     5581 gcaaaatctg ctttggattt tggcgctaat ttcccataac gattaaaccg agaatcatct
     5641 aagaaagtcg tatctgctga ccaattagcc gaataaggtg ggttcattac tactgcatca
     5701 aatgtgtaag gttcatctgt tggccagtcc ttattcaacg tatccccatt gcggagattc
     5761 atttcttcgg cgtcgacacc atgtaaaatc aagttcattt ttgctagatt gtatgtcgtt
     5821 gtatttagtt cttgcccatg atatttaacg ttatctgggt gtgttaagta gttacgtacg
     5881 tttaacatca atgaacctga acccatcgtt ggatcaaaaa cactaaagaa tcgtcgttcc
     5941 ttttgatcga gcgttacgat ttgcgccatc atatcagaaa ccatatgagg tgtgtaaaat
     6001 tctccggctt tcttccctgc ttctgaagca aattgactga tcagaaactc atacgcatcc
     6061 ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat tcaatttctt gatgacttcg
     6121 gtaatcgtca catttcgttg ttgctcatct gttcctagtt tttttgattg gagatccaca
     6181 tcatcaaata acccattaaa ttgattgtag gtagatgcca attgcacaaa ggctttgttt
     6241 agatcgttca attggaacgt tgcttgtttt gcttgatccg ctaagacgtt gaaaagatac
     6301 tctggtgcaa ttgcatatcc caaaatatca acgattgtgg caatcagatc ttcttttgat
     6361 tcttcatctg aaagtaattc acgatacaac attgtttgtt tgcttactgt gtcatattcc
     6421 tctaaggatt catctgctaa aagaaccact tgttccaaca atcgatcgga taaatattta
     6481 tagaaaatca gtcccaataa ataattttta tattctgacg catccatttt actacggaga
     6541 ttatctgccg cactaaataa tttttgattt aattctgcac ccaaaaagat acactcctat
     6601 ctcttattta aagttgctat ataccctctt attttagcat accccctttt ataaaaagca
     6661 aaaaaaaaag aaagaaactt attcttgcta ataaactacg cgaaaggagt ctaacgttgt
     6721 ttgatataac ttagactgat cgttaactta ttttttatat ttctacatag ttatctttag
     6781 aaccatttca gatacatatg aaacaattac atatgatttt ttcataaaaa aacagtactg
     6841 tctagtagta taaaatatac aaatttctta aaaatttttt cactaaaatc actacctgaa
     6901 acagtctaaa acacttatat atcaacgttt aatgaaacta tctccgtttc atttcagata
     6961 caaataaata tagtcgggct ccaaatccga aaaaaaatac ccccagtagc ttatcctaag
     7021 ctactggaga tgtataagta cggtcatata acaacggggt agggtaaata aatgaaaaag
     7081 atggaagaat cattattcaa taactttttt aaatacttcg ttgtaatcag ttacctgtcc
     7141 gttcttaaat accacagaat aatctttttt cccattttca agtaaccgaa gtggaatgtc
     7201 tttaccaact tgctctgtca ctgcattact gatacctgtt agattagcta ataaatctga
     7261 taaagattga gcatctgtag aaatctctgt atcagtattt atcgtgattc ctttatcttc
     7321 atttacaagc ttccaagtag gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct
     7381 ttcatctatc gtcttcccag tatctacttc tgtctcatta ctcttagatg aatcgattct
     7441 ttcatccatc gtctgctccg taactgcttc tgtctcatta ttctcaccgg aaactctctt
     7501 cacacccaaa cttatatatt gtccatcttt agtgtgcata gttaaaattc ccacgctttt
     7561 actaataaat tcactataac cagttctttc aatagagact tcaatgtatt tcacactatc
     7621 atactcaaaa gaaaaataga catgtaaaag cattttgttg tttacatcta cataataatc
     7681 ctttgactct acttgagacc ttcctatacg gaatacagct ttactgtctg atatctctat
     7741 accagcatcc ttattattaa tctgccaatc tccaagtagc tcttctggca agctagtagt
     7801 ttcttgtgaa cactttacca cttcactggt agaggtcgaa acattcacct ccgcactgtt
     7861 agaagaattt tccaaggaat caggagcctt agcacaacca acaagtcttt atatattgtg
     7921 cagccgatat tttcttcatt cagtacaccc ttgaaatatg cagtgttaga taaagcagtt
     7981 cgtcctgatt tgctgaggca cccatctctt cttgaacgta ctccttgatt ttcaaggccg
     8041 cccgatactc tttcggaaaa cgctgcttca ctgaagccag caaaaaatca tccttctccg
     8101 gcaactcttc cttcttaatc attcgggcaa taaaaaactg taaatgcgtc acaaatctta
     8161 agtaaagcat cgaatcttta tcaacctttt tttcgggaaa cagatatcct agcatagcca
     8221 agatatcctt tagtgctctt accgagagca tggtttcttc catatttgct tgttcacttt
     8281 gggcatttac aatgtgaaac gcaatgtttc cagcctcctc ttctggcaac tgaacattca
     8341 tttttttgtt gatcaattgg atagcatagc agccgatctc aaattctttt gggtaatact
     8401 tttgaatttc aatcagcagg cgattttgaa aaacaatgcc tttttgactg cgttcaatgg
     8461 catgcttcaa gtgatcaacc aaggtaaaaa atagactttt attcagctta aagtcaaact
     8521 tggcgttggc ataatcgata attcgcttga cgcaaataat tagatcatcc ggagcagcct
     8581 caaacagttt aatgtaatcc ttttttagag catcagaaag tgaatctaat ttaaatactt
     8641 tttggatttt gtcagccgga accaaatcgc ctttctttag ttgaaatcca atccctttcc
     8701 ccatcaaaat tacttctttg ccttgcttat ctaagcaaag aattaaactg ttgtttaacg
     8761 ttttaattac tctcattaga ttcaccgttc tctctaagct aagccatttc ttgcgattac
     8821 tttttgatac caaaagaagc tttctttttt tattcgtttt aattctttaa gattctcatc
     8881 ggttcggtca acatatataa aaccataacg cttattgaag ccttgatggg tactaacaac
     8941 atcaatcgct gaccaaggta aataaccaaa aacatctaca ccatctgtta aagctgcttg
     9001 aatagccaga atatgtttgg ctagataatc aattcgatcc tggtcattaa ttcgtctatc
     9061 ttcctcaact atgtcgggtg ccccatagcc attttcagta attaaaatag gtaaatcata
     9121 gcgatcgtac aatttcctca atgttaatcg tagtccagta gggtcaacta cccagccata
     9181 tggcgtttta tctacataag gattttcagc tgcccggtag acaccttctt ctcccaacat
     9241 gatctgttga tcccccgctc tagcggaaac atctgatccg tcatttttgc tggcagcgat
     9301 ggtggcagtt gaataataat taatcgcaat aaaatcgggt ttaccggagg ataaaattgc
     9361 ctgatcctct gcctcaattt taggatataa tccacgatca ttcaaatagt tttcaaacaa
     9421 ataattgtat tttccccgaa ctgctacatc taagaaagac cagcctctca atgtttccca
     9481 attatgggct gcaattgcat cttctggact ggaggtagct tgatacatag ccgttaaatt
     9541 gattgctgga ccgatttttc ctttaagatt caactgatga tacaagtgaa ttactctagc
     9601 ttgagctaat aaaacatggt gattaatttg aaagagttct ttttttgaag gcagctctcc
     9661 acttggtggc aaaccaatcg ctccaggatg aaggatcatc gtgttttgct cattaattgt
     9721 cagccaatag ttcaccttgt caccatactc agtaagcaat acctgtacat agtccacaaa
     9781 ataatcgata gttgctcgat ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc
     9841 gaaatggtac atggtgacaa tcggttcgat tccctgattc aggcattcat caattacttg
     9901 atgataaaaa gaaattccct tttgattggt tttattattt ttaatatcca taattcgcgt
     9961 ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct ttaaacagct gtatatcttc
    10021 tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg
    10081 atacttgtca ataatagacg gttgtttccc atcctcctga taggcaccct ctgcctggta
    10141 ggcactaatg gaggcacccc ataaaaactt ctctggaaat gctgaaagct gtttgtattg
    10201 cacaaaaatc ctctccttaa ctaatttgca tcaatggttc gtgttccttt aagcttccgc
    10261 ttgcttcact aattcctgcg taattttcac tgttggtcac taccacaatc actgtatcat
    10321 cgtagccagc gtttctaatg gcagcgaaat cggcgttgat taataattgt cctgctgaca
    10381 cctgatctcc tgcttttaca aatacttcaa agggttcccc ttttaaattc acggtgtcga
    10441 tccccacatg gatcaataat tcaatacctt ctttagaacg tagaccaatc gcatgtttag
    10501 attctaaaat agtaatgact tctccctcaa caggtgaata aatgctgttt tcttctggat
    10561 gaatggctac gccctttccc aatagtccct tagaaaagac agcatcgttc acttcagaaa
    10621 taggacacac ggttcctttt accggatttt caacaatgac tgcttcggct attgccgttt
    10681 gctctgattc atcagaagta tcgtcaaatc caattagata agcaatgata aaggttgcaa
    10741 caaaggcgat tacacacgta ataattgcgt gaacaatatt cattggattt tctccaataa
    10801 aggctggtaa ggctgctaag ccaggggaaa caaaggcata tctgactaaa ccgctcaagc
    10861 cagcatacaa accagcaatt gcaccaccaa tcatggaagc aactaatggt tttttcagct
    10921 tcaaggttac tccatagagt gaaggctcag taattcccat taaagcagtg atcccagtgg
    10981 acacagctag ctgcttcaac tctttgtttt tagttttcgc ggctacagca aaggtagcac
    11041 ctgattgagc aatattagaa gccagcattc ctggcccatt gatcatttca gaacccgtac
    11101 tgctcaattg catagtagcg attggcgtca ttgcccaagc tgtacccgta accaccaaaa
    11161 atggctgcaa accacccatc aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt
    11221 cagcccccat tgctataaga tcgttcagca acgttccaaa cggtccaatg acaaccaacg
    11281 ctaaaggccc cataattaaa aataccagca tcggcttagt aaagaattta ataaacgaag
    11341 gcgaaattcg atccgcaaag ggctctacat aggacatgat ccaaacagta aagataatcg
    11401 gtaaaactga tcccgcataa tctgcagcta ataccggaat tcctagaaaa gaaacggcat
    11461 cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa aatgccagca acagtaatcg
    11521 ccaaaattgg gttgacatca aactttatgg ctgccccata ggctaatagt actggcataa
    11581 aataaaaggc tgcatcagca atagtattca aaatgatgta agtttgacta ctgtctgata
    11641 gtacaccagt caaagtcaag acggccaaaa tggcttttac catcccggca cctgttatag
    11701 ctggaatcat cggtgtaaag gtggtagaaa tgacactaat caatcttgaa ataatacttt
    11761 cttgattgtc tgaagagggg ccctttttaa ttgaagttcc cacttccttt tgaatttcct
    11821 tgaaggctgt ttggacttca ttgccaatca ccacttggta ttgtccaccg ctatccacca
    11881 cactaattac accaagtgtt ttttctaagg ctttcgtatc ggctttcctt ttatctttga
    11941 gttcaaaacg cagtctggtg gcacaatggg ttaagctgac cacattttct tttcccccaa
    12001 cctgttgcaa tacatttctt cctacttctc tgtaatccat tattctctct ccttaaagca
    12061 gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg aagtgttagt ttgtcttggt
    12121 cttgcctgca taatcagtaa catgccaata acgttatttt gctgtaatcc aataatagca
    12181 cattatgata gcgcaatcaa cagggaattt aattttttat cttttcgttt gaccgtatag
    12241 ggtaatataa cagttagatc acatcaacta gggtatatct gcgtatactt actgctaaaa
    12301 taagtatatc taatattaac tataccagta aaaccattcc tacataacca gcgggaaatt
    12361 cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc acacaacttc aacaattctg
    12421 ctcttgggaa aaattgatga atttggtgag taaaggatgg aataaacagc attccaacaa
    12481 tataggcaac aatagtgata cttgtttttt tggtaaaaat cagtaagcaa tagccaattt
    12541 caatcattcc gagatataaa ggtatcgata gaatgatttg ggctagtatt cttaaaaatt
    12601 aactagcaaa tgtttaaact gatttatttc taatctacat gtagtaagta tttcaattcc
    12661 accaattatc aataaaaata ccaaacatat ccataataat tttttctttt tcatttcttt
    12721 tatattgtta taacagtgaa taacataaaa attcccgaca atcattgtaa tatttttttg
    12781 tcacctgtat tagtatttaa tttataggaa actataaaaa ctccctagtg aatttacacc
    12841 tttgggagtt cctgtttgga cggtatgcat aatacattta aatcaattta acattaacac
    12901 ctcagcatat ggtatctcca catatatacg gtctttcttt ggatagtact caatatttat
    12961 gttgatagca cttccattgt ctaatatagc tttagcccgg tatgtgttat ctccattttc
    13021 atgagtaatc gtcacatcat cacatgatcg atttaagttg tactgttctt gtaaaatctg
    13081 tgttaccaat ggttttgatg acttctctaa tccagatgta cctgtcattt ttgaaaaaat
    13141 aatacctata atagccaaga caacaatacc tataatgaca aacttcttgt tcatatttac
    13201 tttattcaac tttttatctc ctatctccaa aataatttgc tcttatccct cttatcagta
    13261 cattgacatc cctaaaagta caccaaagat tgctccagct acccctgcta tcatcaaaat
    13321 cgtacccatt ttctcatctt tttctcgata taagtagccc ataataacag caacagcacc
    13381 aaatacaaca ggaataaatg ctaacgatac taccatagaa atccaaccaa taatcaaata
    13441 cagcttccct gtctcttgtc catgtacgat ttgttccttc atttcattaa ttgtctgctc
    13501 agtacttttc tttactgtgt ctgttccggt tgtctcatct gtttcttgta gtgctccaca
    13561 aaactggcaa tacttacttt catctgttat ttctttgcca cattttctac aatacatgtc
    13621 ttctcctcct aaaatcattc aattgcttat tagttaactg tttactttat taaatacatc
    13681 agaaaacagc tcattcattg gtacacttaa tatctcagaa agcatcttca tttgataggg
    13741 tttaggaatc gttctccctg tttgccacga acttacacat tgctgtgtaa cattcatttt
    13801 ttttgctaac tccgtttgag aaagtccttt ttcttttctg tatcggctga gattacttac
    13861 ttttttcatg attctcccct cctgttttat tatatacaac aatatgatgt atttcaactc
    13921 ataaaatata aaaaacttca aataccatta caaatacaac aacttgttgt actatactga
    13981 gatgggggtg tattaatgaa acagaaacaa tatgcaaagg tcattagaga aaagcgaaaa
    14041 gctctcgggt ggacacaaaa agaattagct gaaaaaatct tctctacaca acaggcagta
    14101 gcaagatggg aaaattctgt aacagagcct aacctcgata gcttaacagc tctttctaga
    14161 gccctaggaa cccctgtaag ccattttcta gacaatgttg tcgtagatta tgaggaagag
    14221 tttttggctc tctatcgttc tcttagcact gaagatgcag ttcgaacaat tgactatatg
    14281 aagctattaa aaaggcaaga aaatgaacgc aatcagctat tgaaagattg aaaaataaat
    14341 aacccaccga actttaacgg tgggtgtatt gagatgaaat aagattaaga gctatggata
    14401 acctgtctag tttaatacaa ttgggtcttt tctcttcctt actctccgtc catcatctct
    14461 tgttacactt gtaaattgat acagaggaac tatcccagct cctgataata tcaattcttt
    14521 tattgtttca tctatccctg cccctttcgc taaatatatt aaaaaactac tacttgcttt
    14581 cacattgtat agtttcttaa tatccttaga agctcgttca agagtgaatc cttgtttaac
    14641 aaagtagcct ctgtttttta ttaaagcatt tttcacgctg ttatccccaa cattacgtag
    14701 gcgttcacaa acagtgttaa taagttcttt ctctgtcatt agatatgtcc cttgccacga
    14761 taagccctgt aaaacttcat gtgcttcatc taaaatcaat tgtttttcct tgctgagttc
    14821 tgatcgggaa ttttgaggaa atactctttt agcctctttt tcgttaaaac atatcttaac
    14881 ccattcataa gaaaaatatt gatatgtaaa atgaattttt tccagaagtg gaaggatatc
    14941 ttcattcaat ttttgaatca cagaagttgc attaaaaacg tctaatcgat agacattgct
    15001 tctcttatga ttaaagtttc ctaacgcttt ctcttccttt aacctattta ggtattcgag
    15061 catcttcaaa ggaacatctt catcatctaa tttttttatc aatcctataa aagtcataag
    15121 tgatagtaac ttattacatt tatctatgtc ataagtttta ccatagtaag aactcatttt
    15181 tttagctaat tgttcccccg acaaaataca agccacactt tccttactat catcaaaaaa
    15241 attaacatca gaaagcaaga catcatagac acgtcgcatt tcctctagat aattccttat
    15301 tgtcctgaaa agtttgtctt tcattgattc aagagtgtca aaaagtactt ttgattcctc
    15361 tttcaatttt cgaaaatgtg taggatacaa gtctaggttc tctaatagcc aattaaatgt
    15421 ttctacccga ctgtctttct ttaaaattag catcacaact ccaataaaat tttcactgat
    15481 atcacaatta ctggaatgac aataaaaaat ggaatgccca gttttactgg taaaaatact
    15541 agcagaggga ttatgatcgt tgtgaaacag acatcttaag tttttacttg acttaagtaa
    15601 tttattcata tcaatggtta acaatctttc ataaatctca ttaaaagaca tgtcggagga
    15661 atcaaacagg cagtctttaa accactgttt catttcttct ttgtcgtcat tttttatcaa
    15721 cttaacaaaa tcggtttcat tgactagttc atactctctc cctgaatcac tttgacaatt
    15781 tttagaacat gtcatggtaa aatcaatcgg aagctcagta atatctaaca tgttgtcttc
    15841 attaattatt atcactgcat ctgacttata tcctccaaaa aacattctat tcgaagcatt
    15901 tacattgtca tcacacccgg ggatgttctt tttcaagtat tcgtaaacag caaccatttt
    15961 accatagtca gtaattgctt cttttaatag aaagacaatc ttgtattttt ctagattttc
    16021 agagtaccga atactttttt gtatcgcaaa cgcagaatta tgaactaaat tgatagataa
    16081 aaccttttct acgcttaagt atctactatc atctttactc aaatctacta ccttcccctt
    16141 ctcatcttta acagtgttat cgacatccag aataattact ctggtttcca caaaactctc
    16201 cttgtcattg cctttattct ttgtcttgct tggaataaat gattgtgatg aatttagtat
    16261 ctctacaaac tcatctcttg taacttctct atagttatct aaacacctat ctcttacatc
    16321 agttttttct ttttttgtaa ctggcttctc taataatttt tcttgatcta cataaattgg
    16381 aatattaatc attgatgttt ccatttgtgt ttcctcctta taaaattctg ttaaagacta
    16441 aaacatacag ataataaaaa caaactcttt attactatat gttttagtct tatttgttta
    16501 ctcaaccttt ataccgttcc atgaaactat caatatcaga ttttttaaat ctaatgaccg
    16561 tatcaatttt tataacaggt aacccgtatt gagaaacata ttttctaatc gttcctactg
    16621 atacacctag atattcactt gcctgctgat agtccatata ctctagagtg actccagaaa
    16681 taatgtcttc tagcataggc tttagcactg taattatctc tttcacttgt tgttcaactt
    16741 gtatagaaac aatgctatca atgatttgta gagacatatt tccccctcct ttcgtgattg
    16801 cttgaacaaa ttttctaaga attatcttcg tattttttgc tcaaaataaa agtattttgt
    16861 tattaaccga ttaatcaaac aaaaaatgat taccagttca tcgacataca cataatacct
    16921 cgataaaaaa ggggtgtcaa cagctttgta aaatttattt acaacacccc ttttttgagg
    16981 tatactaatt ttaggaggtg tggtcaatat gataaaacgt ccaccaataa attatttaga
    17041 acgaaaaaag atactcggaa caaaaataaa agctataaga aagagtaaaa aattaacaca
    17101 accagcattt ggtcttatga ttaacaacgg tcaattaatt gacaaaaaaa ctatctacga
    17161 atgggagaag ggaacctatt taccaatacc agagcgatta tcccgaattg ctgatttggg
    17221 aaatatgagt atagaggagt tagtttgtgg caatgtagaa gaatatattt tgggaattat
    17281 tctttataga gatagcattg tactagatgg tataacattt ccagataaaa acctgttcca
    17341 gcatcttcgt caacagttcc caccagtaca ctctaattta gatacatggc tggatagata
    17401 ttccaaatta gaaccagaga tgcaagaatt tattgctaac aagacttgta ataaagttaa
    17461 aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa gaattattca tcaatgctat
    17521 tgtagaagaa tttgacaata acattctttt cctcactagc agtatagaag aactactaga
    17581 aagaatggta gatgaatggc tacctattca attgaaagac atgtcttatc ccgaagaagc
    17641 tgtgagagaa attactgata atataaacaa gctagaacaa acgatttcat caattgggaa
    17701 aaaatacact aagaaaaaaa tgaagggagg tgataccatc tagtggtaag tactccttgt
    17761 aaactaagag gagatgattt aatggcaaaa atatcaaagt acaaaaagaa aaacggtcaa
    17821 gtggcgtgga tgtataaagg acacattgcc accgatccac gaactggtga aaaaataaat
    17881 actacgagaa gaggttttag tagcaaacaa gaagctcaaa aagattatga cgagtacaga
    17941 catcaaatat tatacggtgt taaaaagaaa gcacctgata tgacatttga agacctgtat
    18001 aatgaatgga taactcatca gcgtaccagt gttaaagctt caacaatcgc aatctcagta
    18061 agatacgcaa ataatcaaat acttccagca tttggtaagc ttaaactatc gaatatctct
    18121 gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag ttacgattat
    18181 atgagaaaac agactgcaca aattttacgc tacggggttg caatgcaata tattgacaat
    18241 aatcctatgg aaaaaacact tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt
    18301 tactcaaaag aggagcttaa taatttactg gatgctttta aagattttgg taacatgaaa
    18361 caatacgcct tcttcagatt attggcatac acaggtatga gaaagagtga agtactcgct
    18421 ttacagtgga aagacataga cacgtttaat aaagaactac atgtcaataa aactcttgct
    18481 gtagatgaat ttggtaaagt tataattcaa tctccgaaaa caagagcttc tagacgagtt
    18541 atctctttag atactgagac tctttctatc ctcaacaact ggaaattaca gcaaaaagaa
    18601 gagtatttaa aacttggata caatactagc tctaaagagc aacatgtttt tacaacagtt
    18661 aaaaacactc tatacattcc aaacactgtt aatgattggt taagatatat tttgaaaaaa
    18721 tataacttgc ctcgtattac acctcatggg tttagacata cacatgctag tttattatta
    18781 gaagcaggag aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg
    18841 acattagaca tctatgcaca tattacaaat aatgcaccta agaaaacagg tcaagacttt
    18901 gctgatatga tggctcatca gtaaaagagc tggtcaaaat ctggtcattt ctagtataca
    18961 ggcaaaacaa aaaagctaga acccttatat atcaagggtt ctagctccta taacaggttt
    19021 acccaacact gccttccatc tcataactga tcaatcgatt gagttcgact gcatattcca
    19081 ttggaagttc tttggtgaat ggttcaacaa agcccatgac gatcatttca gttgcttcag
    19141 attcggtcag gcctcggctc atcagatagt agagttgttc ttctgagatt tttgagactt
    19201 tggcttcgtg ttctaaagag acttggctgt tgtggatctc attgaatggg atcgtatcgg
    19261 atttcgacca ttcgtccatg atgatcgtgt cacattcgat atgagagatc gaacccgcac
    19321 tgtttttggc aaaggttact tgtcctcgat agtttacttc tccgccgtct tttgaaattg
    19381 atttagagac gatagagcta gatgtatttg gtgcattgtg gatcattttt gcacccgtat
    19441 cttggatttg gtttgctcct gcaaaggcga tggagagcat ggtgcctcgt gcaccttttc
    19501 catctaagta aacacttggg tatttcatcg ttgtttttgc acctaggttt ccgtcgatcc
    19561 attcaacggt tgcgccttcg tatgcttttg ctcgtttggt tactaagttg tacacattgt
    19621 ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc ttttcttgta aagatttcta
    19681 cgatggcggc atgcaggctg ttacttgaat aagtaggtgc cgtacaacct tcgacatagt
    19741 ggatgcttgc tccttcgtca acaatgatca gcgtacgttc gaattgcccc gtgttttccg
    19801 cattgatccg gaagtacgtt tgtaatggaa catcgacttt gacgcctttt ggcacgtaaa
    19861 taaaggtccc acctgaccat acggctgaat tcagcgctgc taatttgttg tcggttggcg
    19921 gtacaagttt tgaaaagtac tctttgaata attcagggta ttctttcaaa gcagagtcag
    19981 tgtcagtaaa gacgattcct aacttttcga attcatcttt catattatgg tacacgactt
    20041 ctgattcata ttgtgcggaa gcaccggcta gataagctcg ttcagcttct ggtataccaa
    20101 tcttttcaaa tgtatctttg attttttctg gtacgtcttc ccagtcacga gcgggtttgt
    20161 cacttggttt ttggtaatat ttgatggcat cgaaatcgat gtctgaaagg tcggggcccc
    20221 atgtttgcat tggcattttg ttaaatgttt ctagagattt cagacggaat tcaagcatcc
    20281 attctggttc gcctttgatt cttgacatct cacggatcac ttcttctgtc aagccttctc
    20341 ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa tttatattct tctaattctg
    20401 gtacgcccat gttctcactc ctcttccttg tggtaatgat ggaaagctgc ggtaccatcg
    20461 ttttccattg cttttcctag tgctttccag gcaagtgtcg cacatttgat ccgagcggga
    20521 aatttagcta caccaccaag catagcagca tcgcctaatt ggtcgggatc tttgacttct
    20581 tttccttgga caagctgtag aaagtctaca gttaaatgtt ctgcttcttc tattgatttt
    20641 ccgatgacgg cgtctgtcat catcgaggca cttgccgtac tgatggagca gccactgccg
    20701 tcaaaggcga tatttttgat gattccgtct tttacatcga cttgcaattc gatcacatct
    20761 ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt cagtcaagct tccacgatga
    20821 tgcggatggg aagaatgatc caagatcact tgacggtata aattgtctaa tttagatagt
    20881 cccatgttgg aaaaactcct ttgttgccat gattgcttca actaaacggt cggcgtcttg
    20941 cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct gatacttcaa ggtacttcaa
    21001 tagcggttgc gcacaatgat gacctgctct gacggctacc ccttccatgt ctaaagctgt
    21061 cgcgacatca tggggatgta acccatccaa attgaatgca acgactcctg tccgaaaatt
    21121 cgggtcttgt ggaccataaa cagtcaatcc atcaattgct tgtaattttg gcaacacata
    21181 agcggctagt tcttcttcat atcgatggat attgtccata ccgagttttt gcaaataatc
    21241 gattgcatgg cctaatgcga tagcgccggc gatatttggt gtccctgctt cgaatttcca
    21301 aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa tcgatcattt cgccaccaaa
    21361 ttcaacaggt tccatttctt ctaaccattt tcgtttacca tataaaacac caatacctgt
    21421 tggtccgcac attttgtgtc cgctgaaagc aaagaaatca cagtctagtg cttggacatc
    21481 aatggccatg tgcggggcag attgagcacc atctactacg atgaccccgc cgttttcatg
    21541 gacaagttca gctaattctt gaacaggatt gaagacacct aagacattgg atacatgagc
    21601 aatggagacg attttggttt tgtccgtaat ctttttcgct gcatcctcca tatccaggaa
    21661 gccttcttct gttagttcga tatatttcaa gacagcacct gttcgttttg ccaactgctg
    21721 ccacgggata acattagaat gatgttccat gtaagagagc actatctcgt ctcctgcttt
    21781 gatatagcgg tcgccgaagg ttctggctac ccaattcaag cttgtcgtcg taccacgagt
    21841 aaacagcact tctgctgttt cttttgcatg gatgaacgcc cgtacttttt ctcttgcttg
    21901 ttcataatca tgggtcgcgc gttctgctaa cgtatggaca ccgcgatgaa cattggcgtt
    21961 atcgtgttcg taatagtacg ctaatgcctg gagtacttgt tttggctttt gggtcgtcgc
    22021 tgcattatcc agataaacta aaggttcatc attgacgtct tgaaataaga taggaaaatc
    22081 attgcgtagc tttttagcat cgatcatcca ttcaacttcc cttcgattac ttcgactaat
    22141 tctttccgaa cttgttctac agggattgct gttaagacag aaccgaggaa tcctcgaatc
    22201 actaagcgct ctgcttcttc tttatgcaat cctctactca tcagatagta catttcttct
    22261 ggatcaactc gtcctacact ggcagcatgt ccagcagtta cttcgttttc atcgatcaaa
    22321 agaataggat tcgcatcacc acgtgcttta tcagaaagca tcaagacacg actttcttgt
    22381 tgggcatctg caccttttgc ccctttcaag atgtggccga taccattaaa ggtcaatgtt
    22441 cctttttctc ggatcactcc gtgttgcaaa atatgcccga tcgtatgagg ggctttattg
    22501 gttactcgtg tatcgattcc ttggatttgt ctgcccgagc tgattgccac gattttgact
    22561 tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt cagcgatcac atgaccgtca
    22621 ttcatgacac caattgccca gtcgacagaa gcatctcgta aaatgtgccc acggcgattc
    22681 atataagaag taatattttc tcctaattga tctacggctg aatatttgat ttttgaacca
    22741 gctttagcga tgacttccac gatgatatta cctgatgatt ttgctacttg ttctttggtt
    22801 gtctggaaac gttccagata agaaaattca ctatgttcat ctgccacgat caacacatgc
    22861 ttgaagaagt gctcatcgga tgcgccatct tgaatgaata aggactcgag tggttcatcg
    22921 atcacgacat ttttaggcac atacaggaaa ataccgctgt tcataaaagc tacatggagt
    22981 gcagtcaatt ggtcttcgtt catgtttact gcttttttca tataatattc ttggactagt
    23041 tctggatatt cgatcattgc agtgaacaag tcggtaaaaa tgactccttt ttctgctaat
    23101 tccacaggca gttgttcaaa aatcgtccat gaaccttgtt ggacaagcac aggattgtct
    23161 ttcattgcat caaatgccgg aatcgtgcct gtttcactga tggtttgtgt ttctttcaca
    23221 tcaaataacg accagcgatg gaatttcacc cgatcgatat gcggcaatgg cagttcatct
    23281 gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat caggttcgcc tttacttaaa
    23341 gaaaaatcat ttaccgcgtc aagataatcg agccagtttt tttctttcat tcttgctccc
    23401 ccttacgctt cttcttcttt atagtcgatt cctaattctt tactgatacc agcgtagcct
    23461 tctgcttcca gacgtttagc taggtcagca ttccctgtca tgactactcg gccttccatc
    23521 atgatgtgaa cgacatcagg tgtgatatag ttcaataatc gttgatagtg ggtgatgatc
    23581 aatgcaccga aattgtctcc gcgcatttca tttactcctt tagcaacgac tttcaaagca
    23641 tcgatatcca gacctgaatc gatttcgtct aaaattgcaa atgtaggttc taacatcaac
    23701 aattgcagga tctcattccg tttcttttct ccgccagaaa aaccttcgtt cagataacgt
    23761 tcagccattt cttctggcat atttagtaaa gccatttttt catctaactt tttcaaaaat
    23821 gccataacag aaattttgtc gttttcatca cgtttcgcat tgatcgctgc acgcatgaat
    23881 tccgcattcg tgatacctgg tatctcactt ggatattgca tagctaaaaa taagccaagt
    23941 cgcgcacgtt catctacttc caagtctaaa acatttttcc cgtcaaacaa aatttcgcct
    24001 tctgttactt catagtttgg attgcccatg atagcggcag ataaggtaga ttttcctgtg
    24061 ccgttaggtc ccatgatggc atggatttct ccggttttca ttgttaggtt tacgccttta
    24121 aggatttctt tttcctcgat cgacacatgt agatttttta tttctaagac agacatgcat
    24181 tataccccca aattctcttt cgttctcact cattttagac taattgagaa tgaagcgcaa
    24241 tgaccttcct tactaaaatc aaatatcttt tatttattat taaacaattt tgatttctaa
    24301 attagaatag gtataaaaaa gatgagaaat aagccattac agacttattt ctcatctttt
    24361 ttgttccata ccttattttg catcagcctt tactggttta acagagccgc cccatttgtc
    24421 tgtgatccat tcttggactt ctttgcggtg aagcacttca atcagttttt tgattttgtc
    24481 actgtcttca tcacctttac gagtcgcaat aatattcacg taaggagagt tgtccttttc
    24541 aagcaatacg gcatcttcta atggattcaa ccctgcgccg taagcgaagt ttgcgttgat
    24601 tgcaactagg tcaccttcat cattttggta cgtagaagct aaaagtgatg ggtcgatcgt
    24661 atggttgaac ttcaagttct tagggttttc ttcaatatca tcgaatgttg ctgtttccag
    24721 atctactcca tctttgactt tgactaagcc agcatcttgt agaattgtga tgatgcgtcc
    24781 ccagtctgat tcagaatttg aagtaatgat tgttgcacca tcttttaatt cggaaacatc
    24841 tttgactttt ttagagtaca agcccattgg ttcgatgtgg acagcaccgg cattgacaaa
    24901 gtcatatcct ttttctttga tttctttgtt caaataagga atatgctgga agtaattggc
    24961 atcaatgtcg ccttcttcca aagattggtt cggtaataca tagtcatcga atttgacgat
    25021 ttcaagatcg attccttcat ctttcaataa tggtttcaca tgttccaaaa tctctgcgtg
    25081 cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct gctttatttg ctgaatcttt
    25141 gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc agcaaaagcg ctgctgcaaa
    25201 tccaaaaatt ttctttttca tatcctacat ccccctatat cctattatct tttgtctgtt
    25261 ttcttagtta agaaatcacc gattccttga acgataaata cgattagtaa aataattaaa
    25321 gtcgcaacga atgtcactgt tagattacca cgctggtagc cttcctgcca agcaagacct
    25381 ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa atccgatcat cgagatcgtt
    25441 gtaactgtta atccagaaat caatgcggga atactttcag gtatcaagac cttccagatg
    25501 atttcccagt aacttgctcc cattgcatct gctgcttcca gtacaccagg gctgacttca
    25561 cgaaaagcaa tctctactag acgggcataa aatggtgctg cggataaaac aagagctgga
    25621 atggctgctt gcgcacctag catagtgcct acgattgctt tagtaaatgg aatgatcaaa
    25681 accatcagga tcataaatgg cgttgaccgg aaaatattac tgatgatcga tacgattcca
    25741 taaatgattc gagcatacgg tttgtttttg cgtccaatag agtaaaggat caatcccaaa
    25801 actaatccga tgatcgtcac taagaccatt gagattaatg tcatatatag tgtatcaagg
    25861 atagcctgat tcatcgtttc cgggttgatt tgtttaaaat ctagatatgt ttctgaaaag
    25921 cttttatcca cgatggatca cctcaattcc tactccttgt tctttgatga acacttctgc
    25981 ttgatctaat tctttggttt caccagtcat caagacagtc aatgaaccaa aggaaccttc
    26041 tttcacttgt ttgatctttc catacacaac gttgacatcg atgttatagc gtcgaatcgc
    26101 ttgggaaatg actggctcgt tggcatttct ttcgctaaag gtcaaagttg ccaaacggcc
    26161 gcttggattt tctttgatga gttcagctaa tagttctgtc gaatcagctt ctggttcgat
    26221 gtcttgttgt acaaagcgtt tggttacttc ttttttcggt tgtctgaaga cagtcaatac
    26281 gtctccttct tctacgactt tccccaactc catcacagcg actttgttgc agattttgcg
    26341 gatcacattc atttcatgtg tgatcaagac gatggtcaaa ttcaatttct tattgatcgc
    26401 taacaataag tctaatactt cttctgttgt ttgcgggtcc aatgcgctag tagcctcatc
    26461 acaaagcagg atctcaggat catttgccaa tgctcgtgcg ataccgacgc gttgtttctg
    26521 tccgccggaa agttgtgaag gatatgcttg tccgcgtcct tctaacccga ctaaacgcaa
    26581 cagttcttct gcccgttctt tacgtttgct ctttggtacg ccagctaatt ccagcggcag
    26641 ctggatattt tcaagtacag ttcttgacca taacaggttg aaatgctgga agatcatacc
    26701 gattttcttt cggaatgtac gtaattcttt gttttttaat tttgtaatat cttgatcgtt
    26761 tacgatcact tcgccgctcg tgggaagctc aagtccattc aggagacgga ctaatgtact
    26821 ttttcctgcg ccagaatagc cgacgattcc ataaatatcc cctttttcaa cgtttagtga
    26881 aacgtcattg acggcttgga tgtttccgtt tttcccattg aatgtctttt tgacattcgt
    26941 tagttggatt aatgacatgc cattcacatc tttctcttat gatttttcaa acaaaaaagc
    27001 cttcgccctc ttgccttaag caaaaggacg aaagcttgaa cttccgtgtt accaccttga
    27061 tttaccgtta cttcgcaata acagcctctt ccagtacttg gacttagctt ctttccgtat
    27121 actgtcgcgc tgtaacgggc actcccgtag taagtttgct tacgctcgct tactctgctc
    27181 taagaccatc ttccatctca ttttcgttac ccattttcag caaacagggc tctcttcaaa
    27241 cgtccgtgac atgtactctt cttttcaaag ctttctcgtt tcatatagga ttgcttatga
    27301 ttatagcaac tggaagaatc agtgtcaact tttttcaagt gattttctga ttttataatt
    27361 cgtcaaattc ttttggatca acatctttga aacttaagat ccacgcattc atttcatctg
    27421 aatcattcaa gatattgata tcttcaccga ttttatcatt aacagaagaa actgtacccg
    27481 ttagcgggct ggcaaattca ttgaccgctt tttccgcttc aacttcgatc agaggttcat
    27541 ctttttttag cgattgtcct gcttttggca gattggcaaa cgtgatgtct ccaagttctt
    27601 cttgcgcttc atttgtcaaa ccaacacagt attcttttcc attaaataag atccaaaggt
    27661 tgtccttttt cttcaaatgc ttgcctgcca ttttatatca gttctccttc ataaattttt
    27721 tcattgaatc caattgcaac aagctggtcc tctcgtaaga tcagtggtcg cttgatcagc
    27781 ataccatctg tcgataagac ttggctcgct tttgaagccg taaactcgcc tacttgatct
    27841 ttcagtccaa gttcccgata tttcatcccg cttgtgttga acaggcgacg gattggtact
    27901 tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg gtacttcttt gatcatatca
    27961 acggtttgat aactaacgcc atgctggtct aaccacgctt tggctttttt gcatgttgaa
    28021 catttcgggt accagtaaaa ggtgtacaat tttcttacct ccttgaagat cttgcaggtg
    28081 cttcattttg gtatctcatc gataatatca acccgatacc gatcatgttc cctaaaatcg
    28141 aggatcctcc ctgactaatg aaaggcaatg ggatccctgt taaaggaagt aatccgatat
    28201 ttgctccaat attctcaaag acatggaaca gcagcatcat gatcaaaccg cttgcgatat
    28261 aggcgtaaaa ttcattattc gtatcgaaac acacccgaat catacgatag attaaaataa
    28321 aatacaagaa aatgacgaat gcaccaccga taaagccgaa gttttcaccg ataacggtaa
    28381 aaatcatatc ggactcacga acaggcacat agatattgct tacgttgaat cctttaccaa
    28441 acaagccacc ggtaccaatt gccaaaagtc cttgggccgg ctgataactc attcctgacg
    28501 tatcatggaa agggtccagc catgaatcaa tacgtgcaaa ctgataagat ttgaatccaa
    28561 ctttataaag caagtctctt ccgccttcag tcgtaaccaa gaagatcgtt cctgctccaa
    28621 cgactactgc caagattact acaggtgcga tgatgcgcca agagattcca gacatcagga
    28681 aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc aaagtcatct tgggccatga
    28741 tcaatgctaa aaccggtatt gtcacgatca agagcttccc aatcagccaa aagtccgatt
    28801 tcatcgtccg ttcttttact tgcgtattat gtttagttac gatcagtgcc atcatcaaga
    28861 tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa cgcaccgaat cgaaaccagt
    28921 ttttagaccc tgttgaagca gctaaattct gatcatagaa tttgagcaag agtcccatta
    28981 ccactaaccc caaaccataa aggtaaggag tcagtttcca gatccatttg gaattcaaat
    29041 gcatgatgac aacgattgct gccgctccaa ctaaatacca gacaacttgc atccctacgc
    29101 ctttcaagac tgctggattg ttcggatcat ggctaagtgc tacatataat gagagcagac
    29161 caatcaaaca taacagaaag actggtaaaa tcacgccata atcaatacga ttatcgttcg
    29221 aagcttttaa ttttttttcc atgtttcatc ccttttctta aaaaccatac tgctgattat
    29281 tatagcggtc cagttagata atggaaagtc tttccataga catttgggaa aaattaactt
    29341 tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc ctactgctta tctttagtgt
    29401 atcatagaaa tttttttgat acatgcaatt atctaccagc tgagattctc ctcgctgtga
    29461 gaagtttctg tgctttcatt gtatcgaagg atctcttcgg caatctctag cagctgatca
    29521 gaaaccagat tggttcgttg gatgaaataa aaatatcgat ggaaattcaa cggagtcatt
    29581 ggcacatttt ctggaacaga gcgcttagac aaaatcgagc gaccgattcc ttccttcagc
    29641 aacgcaacaa ttacatcgtt acttttcacg atcatcttct tatcttggat attcatctct
    29701 tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg cttctcttac aagccacaat
    29761 ttgctttttg ggtccccagc gatgactagc tggtcttcca tcaaagagta tctttgtacg
    29821 ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg attcatgttt cgctacttgt
    29881 tctaatgatt catgagaatt cgtgatttcg atctcaaaat gcactttagg aaaccttttg
    29941 atcagatgtt tcatcaactg aggcaaaaga tagacagcaa aggtgtggga agcaacgatc
    30001 cgacatactt cttcttcatt ttgttcattg agcaattttt gctgagtttc ttcccattct
    30061 tctaacaaaa ccagtgtccg ttcatataat aagtcggctt gcttagttgg aagcatctct
    30121 tttcttccgt tacgttgaaa aagatcgata ttcaattccc gctccaactg tttgatttga
    30181 ttcgagactg ctggttgaga taaaaacagt acttctgctg ctctggaaaa atttcttgtt
    30241 tcatatactg ctcgaaaagt cgttaataac ttaaacatgg caccacttcc ataacttttt
    30301 atgataatta ctataaaaac tatttgattt aaatatacaa aaaaccatct ataatagaaa
    30361 tgtacaacaa aacagttgct gatccaacta gtaacttaga tgaagacaat cagctgtctg
    30421 cttgctcgta atcatacatt cctattcatt ttacaatgta ttcgttcatc ttggtactaa
    30481 aatctattaa ataaaaagga gaatattata tatgaagaac tttttgaaaa ttattccgat
    30541 cccgatctgc gggttgatcc ttgggttagc ttcccttgga aatctgttta aagactatca
    30601 ccatgttgct ttaggcaatc taactggcgg tatctctatg attcttatga tattcatttt
    30661 tgttaaattg tttatcttat tcgaacacac gaaacaaacg ttgcatgatc caatcatcgc
    30721 ctccgtttca ccaactttta cgatgtcact catggttatt tgtacttatt ttgtttcata
    30781 tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc gctgttatct tccaagtgat
    30841 ccttgtcttt tatttcaatt atcaccatgt tatcaaagct gatctttcaa ttgaagcaat
    30901 ttatccaagc tggtttatca tttacgttgg atttggggtc atcactgtaa ctgccgggaa
    30961 cttcttccca cttattggaa aaattttctt ttggatttca ttgatctgct atgcgttctt
    31021 attgccgatc atcatcaaac gtgtcttttt cgtaagaaat atggctcatc cgacacgtcc
    31081 gctgattacg atcgttgcag cacctggttc tctttgccta acaggttatc ttaaaaactt
    31141 tgctgaacct aatttttatc ttgtcgtagc attgttcgct ttatctcaag tattgtattt
    31201 cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt ttttatccaa gctatgcagc
    31261 ctttacattc ccgcttgtta tctctgcaac agctatattt actactgttc attacttcaa
    31321 cagtttaggt atctatagta gcgtattgga tttcttaaaa gttgcggaat gtctgtttgc
    31381 aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt ttcttaatta aagaacatag
    31441 caaacatgga aaatttgttt ctaaaaaaat cgcttaataa tcgattcaat aggttgtgaa
    31501 aaagctgttc tgcatcaagt aataagaacg gccaaacaaa aacagctgtt catgtttttg
    31561 tactggttgg gcttattacc gcagatgcaa gcttttgaac accgtttacc aagaggtcgt
    31621 aaaagaagtg gtcagcttca agaaataaga aggaatttac gaaaattgct tctcaaattt
    31681 ttgtgaaatt tcggcttatt tccgtagaag ctacttctgg aacaccgttt attcgaaaca
    31741 agaggtcgta caagactttt gtcacgacct cttgttcttt tgtcaaaata ctggtttagt
    31801 gctactatgt tcgagtatgc ttttagtatt tattgacgaa aggaaccgat caaatgaaat
    31861 cattgtcaca tccaataaaa tctgtaaagg attcagcggt ccgcgaaatc ttacccggtt
    31921 tactgctttc ttttgccatc gctgccttca gcaggcttct tgctatttat ctgccacagt
    31981 taggtggagc gactattgct attttattgg gtattttatt aggaaatact ttttttaaac
    32041 agcctgttct agcaatcggc acaaaatttg ctgaaagcaa actattagaa ttctctgttt
    32101 ttttactagg tgcaactgtc acagctcaaa cgattgctca aattggtgta aaaggaattg
    32161 tttttgtgtt actgcaaatg tcagtcacta tcatcggtgc ttacttgatt ggtaaaaaac
    32221 tgcaattcag cgatacttct tctttgatga tggctggagg aaacgctgtt tgtggttctt
    32281 ctgctattgc ttcaatcgcc ccttccatcc atgcgaaaga ggaagaaaaa ggacagatca
    32341 tcacactagt caatctactg ggtactgtga tgatgttgac tctccctttt cttggattac
    32401 agttgtttgg tggaaatctc attgaaaaaa gtgcactttt gggtggtaca ctccaatctg
    32461 ttggtcaagt agtcgctggg gcaagcttac ttgacaattc tgttgtcgaa ttcgccatgc
    32521 tatttaagat tctacgtatt atgatgctcg ttgttgttgt ctatctgttt gaaaaattca
    32581 tgcacaagaa acaaccagct ctgtttgaaa cgccaactga tacgcagaaa aaacgaaaat
    32641 taccgcttcc ttggtatgta ttagggtttg tgatcttttg tttggtcaac agcttgtttt
    32701 ctttacctgt agttattgac caatcggcac attttatcag cagctggttt gagatcactg
    32761 ctttagcggc aattggtctg cgtttagact ttgcaaaatt catgaaagaa ggtacacgct
    32821 tcttatctta tggattagct gtcgggcttc tccaaacagt gatggcttta ctatttattt
    32881 ggctgatcaa agtttagaat gaacatgaaa aaacagttgt gattctcgca tggaacaatg
    32941 tccgaatcac aactgttttt aattttgcag atatccttct aacaacaaca attggcgttt
    33001 gatttcaacc ccatctattg cgtttcccat atatcctgtc agttttccat tttgcccgat
    33061 cactcgatga caaggaataa cgattggcaa tggattggtt cgatttgcct gcccaatcgc
    33121 tcgcacagct ttcggactat caacagcaac ggctacttct ttatacgttc ggatttctcc
    33181 ataaggaatg gtcgacaaag ctttccagac attttgctga aaaggcgtac ctctctttat
    33241 agataaagga acatcaaatt cttttctttt tcccgcaaaa tattcttcaa actgttcttg
    33301 tgtgcgcgtt aaaattcgct gattcgctga ttcgctttct gaaattggct gaaaagatac
    33361 agcagtcaat ccggattcat ctgcatctaa ccataaaaag ccgataggtg tgtgtatttt
    33421 cattttgttt gctcctagtc aatagactgg aacttcctcc tttttctcta tccaaaacta
    33481 cttgccaact aataaaaatt gttcttaaaa gtttgataaa ccgaggaaac atggtaaact
    33541 aaatacgtta gtttacaaaa agaatacctg ttcttttacg taaaatcaaa ttttaaaggt
    33601 gttatttaag gtttattaaa ttttgaatgt aaaccatata taaacatacg attgaaagga
    33661 tagaaacgat gaaagtatca attgttgtcc catgttttaa tgaagaaaaa actgttcctc
    33721 ttttttttga tgaggttgaa aaattcaaag gtgcccatga gttcgaatat ttgtttattg
    33781 acgacggatc aaaagacggt acgttagcag caatcaagaa gctagcctca gcttatcctt
    33841 atgtccgctt tgtctcattt tccagaaatt ttgggaaaga agcggctctg tacgctggat
    33901 tgcaagcttc tacaggagat ttagtgaccg tgatggatgt tgatctgcaa gatccgcccg
    33961 aactgcttcc tgagatgatc aaccggatca ctacttctga tatcgattgt gtcggcaccc
    34021 ggcgttcgac tcgtgaaggc gagcctgcga tacgcagttt ctttgcgaaa aaattctacc
    34081 agttgatcaa taaaatcagc gatacagaaa tggtcgatgg cgctcgtgat ttccgcttga
    34141 tgactcgtca aatggtggat gccgtcctag aattaacaga atacaaccga ttttcaaaag
    34201 gattatttag ctgggtcgga tttaaaacag aatatatttc ttatgaaaac cgcgaacgtg
    34261 tggctggcga aacatcttgg tccttctgga aattattcaa ctactcgatt gatggtatcg
    34321 tgaacttctc ggatgcaccg ttaacgattg cttcatttat cggcgctttg tcttgtgtcg
    34381 gttctggtat tgcaattttg ttcattattt tgcgggcatt gttgtttggt gatcctacat
    34441 caggatggcc ttccatggtc tcgatcttcc tgttcattgg tggaatccaa ttgttatgtc
    34501 ttggaatcat cgggaaatat attgggaaaa tcttcttaga aacgaaacag cgtcctgttt
    34561 atatcataag agaaacagaa aaagtgaagc caaatgaatt gaagcctcac caataatata
    34621 cgtcaaatga agtaaacatt tttttaaatg aaaaaggact gtgacaaaag tcttgtcaca
    34681 gtcctttttt ccgaataaat ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    34741 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
    34801 tctatcacaa cctctttcat atcttattac ttcttcattg ctgcttttag tgctgctgct
    34861 aatgggcttt cttctggttc ttctgcttgt gcatattttt tcagcaagcg tcgttcttca
    34921 tgtttggtca ttttcttctt ctgctctttt ttatcaagca atttttctgt cgttccatcg
    34981 tatttacaac ggaaataagc accgttttta ccttcgatga tttccatttt ccggtgacat
    35041 tgcgggcatc gatgattaga gacttttgga tcttttcttc tgcggtagtt gcaatctgaa
    35101 ttggtgcaga catagatttt gccgtcacgt gtgtttttct cgcgcagatt ggccccacat
    35161 tctgggcatt tcttttgggt aatcgagaaa tcttgatatt cttgtttgct tgatttgatc
    35221 tcattcacca agcgttttgt ttctttttcg attccattca agaaggttcc tgcttgtttc
    35281 tttcctgcag caatagcttc aaggtctttt tcccagcttt ctgtcaaatc tggtgttact
    35341 aaagagggat ttaccaatac taataattgc ttacctttag gagatacttg cagtccttga
    35401 ggggtacgtt ccatcagttc agatttgatt agtttctcga taatctctgc acgcgtagct
    35461 ggggttccca gactgtattt ttccatttga ccaagcaagc ttgcttctga caatggttta
    35521 ggcggtgcag atagttcttt tttgatggcg aaatcgggat tgattctcat tcctttttcc
    35581 catttgactg tttccgtctc agtttcagtt tcgcctttcc atcctgcttg gacgacacga
    35641 ttttgccgaa aaatgaatcg ttctttacca aattcaaccg taacttttgt ttggctgact
    35701 ttatggggtt gagcaaacag tcctaaaaaa cgagagacaa tcatttgata gatttttcgc
    35761 tcttcattac ttaatttttc ataacgtgct ctgttttcag ttggcaaaag tgcatggtga
    35821 tcggtcactt tactgtcttg gaatactttt gtctgttgga cgatggctcc gtttttcaaa
    35881 tactgcttga cctcaggagc aaagtcgctg actgcttgca atcgttcttt catcgtactt
    35941 ttcatgtcat ttgtcagata tttgctgtct gtacgcggat aagtaacgat cttatgggtc
    36001 tcatacaaac tttgtacaag acttaatgtt ttctttgcag aatagccata gcgttgattg
    36061 gcttctcttt ggatctctgt taaatcataa ggcaatggcg cagattccgt ttttaatttt
    36121 tcttggactt ctgtgacagt tcctttgctt gtactgatca attgaacaag tttttgtgct
    36181 tcttcttgtg tttttaaagc aaattgattt ttttgaagca ttttggcttg ttcttttcca
    36241 gcttgcaacg tgatcgtgaa atacgtttgt ggacggaact gctcgatttt cttttcttgg
    36301 tctctcacta atgctagtgt cggtgtctgc acacggcctg ctgataaatt atccgcgtac
    36361 ttcacagtta aggctctggt cacattcaat cctaccagcc agtccgcttt tgcccgggca
    36421 agagcagatt catacaatgc atcatattct ttcgcaggtc ttagttgctt gaatccagtt
    36481 ttgattgctt tatctgtttg agaagagatc cataaccgct tcactggttt attgaaatgc
    36541 acatactcta agatccagcg agcaacaagt tccccttctc ttccggcatc tgtcgcaatg
    36601 actgcttctg agacatcttt tcgctgagcc agctgcttga ttgcttttaa ttgatgtcct
    36661 gttttaggaa gtggtttgat tcctagtttt ttagggatca ttggcaacgt gtccatttgc
    36721 cagctctgcc attccttatt cagatcttcc ggcattttca atcctaataa atgaccaagg
    36781 gcccaagtaa cgatcacctt gggtccttca taataatttt tgtgcttttg attagcacca
    36841 agcaccttgc ttaggtcttt cgcaacgctt ggtttttccg ctagaattag ttgtttcata
    36901 aaattccttt ctgctttcta ctgtgatggg ctgatgattt ttcaacaacg caagtccctt
    36961 gtattgctgt aaatcatcgt cacactcttc acaccagcgc tcatctccgt cattccaaaa
    37021 ggcgctgaga aagttacttt ttgcattgat atattcataa ttttgatgga tttcgttcca
    37081 ttgtttttcg tacgcttgtc gtgcttcttc gaaagactcg aatgtttctt cttcttcaat
    37141 atcttcttcc cagtcttcaa aaaaccacca aggctcattg tccccgaaca tggtaattac
    37201 ttgatacata ctcgtgctcc ttctttcagc ttttctagac tcatcttagt cacttttcaa
    37261 gaaagttgca agaattttct cttataaaag ggtcatttaa tgaacatctt aaaattgtta
    37321 caaatgaatc tgattgttta aggcaaagta gcgataaagt tattgatcat ccaaaaatag
    37381 ctgtcttctg gatcgattgt tggttctttt gtaaagtatc ctaaaaaacg taaggaaaga
    37441 taaccgtgga tcatactacg aagttccctg cttcgatgaa ctttttcttc tttcgttaat
    37501 tgaaaagcct caaatatctg caaaatgatc tgattcgttt catggattcc atctaataaa
    37561 acagcttcat tggtatgcgg tacactgatc agtaactcat acactgcttg atttgaaaaa
    37621 gcaaaggatc gataagtttc tgcatagatc tgcagtgctt cctgaccact ttttcccaca
    37681 agagcccgtc gcagctggtc attaagttcc tgcagaagtt tcgctgttag cgctgttttg
    37741 acatcacgaa tatttttgaa atggttatac agcgagggag ccttgatatc caagcgtttg
    37801 gctaaatgct gaaaggttat ttgctgaaca tccatctctt ctgctagttc tcgaaagcag
    37861 tcgatgattt tctcttgaga cagatttctt tttttcattt ccatgtttcc tctcataaaa
    37921 taaatccttc actctttcag tttaccacga aaaagaaaga gaaaaaagag actttcattg
    37981 ataaactaca tattgtagtt tataataaag agctgtagat gaaaattttc agaaagaagg
    38041 agtctaattg gctgcaagaa taaaggaaaa attatttagg aaaagagaaa cttgggaaca
    38101 aaacttgttt gtcttatggt ttggaacatt tatggctggt atcggattta gtttagtcat
    38161 gccttttatg tcactttaca tagatacgtt aggtaattat agtacgtcac aattgaactt
    38221 ttggagcgga ttgacttttt catctacttt cttggtgact acgcttatct ctccttggtg
    38281 gggaaaatta gccgaccaaa aaggaagaaa attgatgctg ttgcgtgctt cattaggaat
    38341 ggcaatcgtc atttctttga tgggttgcgt gactagtgtg tatcaattag tcgcattgcg
    38401 tctgcttcaa gggatttttt ctggttatat cagtaatgcc actgcccttg tcgcaactgg
    38461 gacgccaaaa gaaaaaagtg gtcaagttct tggaacctta gcaactggtt ctgttacagg
    38521 aacattactt ggaccgcttc ttggcgggat ctctgcttca gcgtttggct atcgccctac
    38581 ctttttcatt acagggatca tcttattttt cgtcttcttg ttgagtctgt ttttcgtcca
    38641 tgaaaaattt gtcccagtga aaaaagaaaa catggtttct gccaaacaga ttttcaaaga
    38701 cttgaaatat ccacacgtcg tcatcggtat gttcgtcaca acgatgatca tccaagcatc
    38761 caacaattcc attagtccaa tcatcagtct gtacattcgc caattgctgc atggacatgg
    38821 gaatgttacg ttgatcagcg gaatcattgc ttcgatacct gggattgcca ctttgatagc
    38881 cgctccaaga tttggtcgct taggtgacaa gattgggagt gaaagaatac ttgcgattgg
    38941 tttgggtttt gcgattctgg tttatatccc aatggcattc gtcacgaatg tttggcaatt
    39001 agctgcctta cgttttttga ttggtatctc tgatgcttgt ctgctccctg ctgtccaagc
    39061 attgatcaca aaatattcgc ctcatcaagc tgctggacgg atcttcagtt ataaccaatc
    39121 tttccaagca actggtaacg tagtagggcc tatgatcggc tccagtgttt ccagtgcatt
    39181 tggttatcgt ggcgtctttc tttcgacttc tcttcttgtt ttggtcaact tcttgcttgt
    39241 acgacacaat acacaagaga tccatcagca agaaactgca gaagaaaaag cttcaggagt
    39301 ctccatagct tcagggcact ctccttctca tagacaataa gcgtaaatga aaaacactgc
    39361 ttgaacagtt gtcacagact gtccttgcag tgtttttgtt taggcctgtt ttttcaagaa
    39421 ctcagataag aggaataatc ccttttccaa ggtttctttt ttacaacagt aacctagtcg
    39481 ggcatgcttc ggtaaatcaa aggcacttcc tggtactaat agtactcctg tttcttctaa
    39541 taaattgata caaaatgtct ggtcatcttc ttcgatggct aatttaatga aagaagtaga
    39601 gacgtaattg ggagcgacaa gttcaacttt cggttcattg gctacccatt gttttaaaat
    39661 cacaaggttc tctgtaatga tctttttgtt tcgcgccaat attttttctt tattcttcaa
    39721 tgcatgtaca gcaagatcat ctgacaggac tccgccgcaa atcatcgtgt agtctcgata
    39781 ctttcgaaaa acttctgcca actcaggtcc agtagctgtc cagccaatac gaatacctgg
    39841 aatggagtag gttttggata gggaattggt tgctatacct ttttcataga catctacgat
    39901 cgacaagaat tcacctttat ccgttaaagg cgcatatact tcatctatca atacatatgc
    39961 atctactgta cgagcaattt ctgcgatttt ttctaaagtt ttccgatcta gcaatgtgcc
    40021 tgtaggattg tttgcactat tcaaacagat gatttttgtt tcaggtttga ctaaggcttc
    40081 tagctgttct aaatcaaact gccaatcttc ttcttccttc aaatgaacaa agtcaacagc
    40141 ggcacctaaa gatttgggga tgtcatataa ttgctgataa gtaggaagca tcgaaacaac
    40201 atggtctcct gcttccacta acgcgtatag agctaaaaga ttagcaccag tcgctccatt
    40261 cgtttgtaag atgttttccg gatcgacggt ttgataaaga gaagcgactt cttgtttgaa
    40321 aacatctgat ccttcgatcc agccatagtc caatggttcg tttttgcgtt tttcgaaaaa
    40381 tttacttacg ctagtcccat ctaagccaac gagttcgtct aatgtcaatg cttcgatcga
    40441 gctttgtgca atatcatata ccgctttctt ttcccattta tttagccatt cttctacacc
    40501 gaaatttgcg attttcatgt ttctttcccc cttatatctt gatccagcct acagcaagca
    40561 acccgattga aagcagtgcg gccacacaaa tcagcccaat cattactttt tcttttcccg
    40621 taatttgatg tttggtttca cgacaagcga tccagtagaa gcaaaaacca ggaagatagg
    40681 caatactaca taaaaggaca taagaaaatc ctgctaaaat gatgcccatc aattcaaaaa
    40741 ctacagcaat cccaccgatc actacttgtt ttttgattcc ttcttttaaa gaaaactgga
    40801 cttgatagat cccgacaagt agataacaaa tcaaaattgc cgctgtacat aaagaatagg
    40861 caaattgata ggcataatca gtaactaaga aagtcaataa gaatagatta gtcaagattg
    40921 cagtaacaac taacgaataa gttggggcat tttttttatt cgtttttccc caacctttcg
    40981 gcaacagacc gtctcgagcc atcaacatcg ttgtttctgc tgggagcatc gtccaagaca
    41041 accaagaacc aatgatcgaa ataatcaagc cgatattgat cagcgctgct ccccaatttc
    41101 ccacgattcc ttttaaaata tttgccatag caggttgtga aatagttgct aactcttctt
    41161 gagataagac gccgtatggc aagatcgaag caaggatata gataaacaat aaacaaagca
    41221 gaccaatgat cgatgcttgc tgggcttctg aacgtttctt cgcgcggttg gctaaaacac
    41281 ttgcgccttc gatccctaca aagacccaca tcataaccat caaacaacct ttaatctgca
    41341 accagatcgt tccttgctgc cctgttccat tcactagatt atcagatacg ttaccccaaa
    41401 aatcagcagt gaatatattc actttaaatg cgataaatac aaatattaaa aataggaata
    41461 atggcactaa tttacagatc gtcacgattg tattgatgac gctcgctcct tcaactccat
    41521 tgtttactaa taaagtcaga ccccaaataa aaatacttgc aaataaaata gaaggaacat
    41581 tttgtccacc tttgaatatc gggaaaaatt ctcccatggc gctcatcagc atcgttgcaa
    41641 aagcaacatt tcccagccac gcagacaacc agtaacccca accactgatg aagccaccta
    41701 atttaccaaa tgaagcttcc gcgtaaccga aaataccacc atctagatct ggacgctttt
    41761 ctcctaaatg attcagcgat aataccaaag ctaaaatacc aatcccaacg attatccatg
    41821 aaaggatcgc tggccctgga gctgcagaac tagccagatc acttgtgatc ccaaacacac
    41881 cgcttccgat tgacgaactg acaaccagag caaccagtcc ccatttgcta atacccgtcg
    41941 atttcttttc catttccatc accctcactt ataaaattga acaaatcact tgcaaaagat
    42001 tcccttctat tttgcaactg ctcttgctta acaaaaagca actttattca tattattgga
    42061 tattatgaac tttttacaca agtatttcaa gtataaaatg aataaaaatt aattttattg
    42121 caaaaaaaca gctatgctat ccaaaagaaa aggtgtttct tttgttagcc atagctgttt
    42181 tgattcattt aatatcgctt aaacgtattg taaaactttt gtttcttgat atgcgtggtc
    42241 gatcaaacca ccacccagac attccattcc atcataaaat acaactgctt gtccaggagt
    42301 gattgcacgt acaggttcgt cgaatacgac ttctgcacga ttatcatcca ataagcgaac
    42361 tgtcacagga acgtcttgct gacgataacg gaatttcgct gtacacttga attcttttgg
    42421 cattggttca ttcgtcgtaa agtggatttc acttgcatcc aagcttgtgg catacaatgc
    42481 aggatgatgg aacccttgcc ccacatataa tgtgtttgtc gctagatctt ttcctacaac
    42541 aaaccagggt tcttgcgaat ctcctccgcc accgatccct agtccttgac gttgaccaat
    42601 cgtataatac atcaagccag catgttgtcc tttgacttca ccatcaagag tgaccatatt
    42661 tccttttttt gctggcagat agttgctcaa aaattgtttg aagttctttt caccaataaa
    42721 gcagattccc gttgaatctt ttttcttagc agtcgcaagg cctgcgcgtt cagcaattgc
    42781 ccgtacttct gatttttcca tgccgcctag agggaacatc gtttttgaaa gctgttcttg
    42841 tgataattgg ctaaggaagt aagtttgatc tttattattg tctactccac gaagcatgtg
    42901 gaccacgccg ttttcatcgc gagtgacttg cgcataatgt ccagtagcta cataatcggc
    42961 gccaagatcc attgcataat ccaagaatgc tttgaatttg atttctttgt tgcacatcac
    43021 gtctgggtta ggtgtacgtc ctgcgcggta ttcggctagg aaatattcaa atacacgatc
    43081 ccaatattct ttttcaaaat tcacagagta ataaggaatc ccgatttgag cagctacttt
    43141 cgctacatcc ttataatctt ccgtggccgt gcatacaccg ttttcgtccg tgtcatccca
    43201 atttttcatg aatattccga tcacgtcgta tccttgttcc tttaacaaaa gtgctgttac
    43261 agatgagtcg acaccgccgc tcatccctac gacaacgcgt gttttgctgt tgtctgtcat
    43321 gatatcacca tcatttcaat tagattctga aacatctacg agttgaaggt cgcaactttt
    43381 cagtatttta cattatacgt catatttctg aaaatttcat cccttaatta tcaagtcgta
    43441 aaaatactgt tctactggac ctattcagta cacaaacatg atagtcaacc aaaaaatcac
    43501 tcatcttttg agcattcgat ataaaaaatt ggcatactca taaaaagggc cagcctttcg
    43561 gcaataagga ataattttat aaattgtgaa aaactatttt tcaaaattgc ttcttatcgc
    43621 ttaaagctgc acgtctcatt tacaaccttt tctttttaga tgttatgagt tgctcagata
    43681 agtgattttg tttactctaa gaaaaaaggg aggcaaaaga tacatgaatt actttatcag
    43741 tgatatgcac tttttccatg aacgcttatt aggaaaaaac gactttgctc cgcggctctt
    43801 tgcaaatgtg gatgaaatga accaacagtt gatcactagc tggaatgcag taataaaaga
    43861 aaccgaccat gtttatcatc taggcgacct tgctatgcat ccgcaatatg aaaaagcaaa
    43921 tgcagaaatt ttggaactgg tcactcaact gcaaggaaca atccacttga tcaaagggaa
    43981 ccatgacagt cgcgcttttt tccgttattt agaaaaaaat gatcctaggc ttactgacgg
    44041 aagtcccaaa ttttattttt atgatgtagg tgccatcgtt aaattcgacc atcaacaata
    44101 ttacttgact cattatccga tgttgctcgg gcctaacgac actatccgca atcttcatgg
    44161 acatatccat cattacagcg tacctatcga gaacgatgtc aatgtaggcg tagacgcacc
    44221 ggaaagagaa ctcttaaagg aaaaactacc atttggcact ccgttatctg agcaacaaat
    44281 caaagaaatc tatgaagcaa agaggaagta cgataggtcg tgaaaaaact gccctatatc
    44341 aagtattaaa caaataaaga taattactca tatttttcat ttgagtaggc ttattgccac
    44401 aggaacatta ggatcagaaa tggagttgta acaagacttc tgtcacaact ccattccata
    44461 ctattcgtct ttttgctcat ggtgttcttt taccagtttt ttctgtaatt ctttatcata
    44521 atttcgggca gctgacgtta aaaataaaac gattccgcat aataaaatcc cgattccgga
    44581 aaacaagaac attttttcta cccctaaacg atcagctaat ggtcctgcaa ataataatcc
    44641 aattggacct gtcaggttca tcaaagagtt gaagttccct aagacacgac ctaaaacatt
    44701 cgattcataa ctctgctgga tcattgccat cagcagtgta ttgaaatatg gcgttgcaca
    44761 acctgctcct gcattcaaga taagaaagta caagaaccct tgactatcgt ttggaaccaa
    44821 tccgcttgca ccgatcgtca ggcccataat gatgtaggct ataataatag gtttcattcg
    44881 gtctttccat gttcctataa aactgatcag tgcaccgcct aagagcattc cgactgcgta
    44941 gactacttcc actgctcccg cctgtccaac ggaaccacca aagtaatcta gcgtcatcaa
    45001 tggatacaga ctgattgctg gcatgaacaa cagcatcaca aatgcaccat tgatcgttat
    45061 gtaccataac cctttattat ccatcaattg ctgcagccca aattttgcat ctttaaaaat
    45121 agtaagcgga acttccaata tttcaggaga aacttttggg atcttcacaa aaatcaataa
    45181 gccgacacca aaaactgccc ccaatacatc taataaaata aggtaattga caggaacgac
    45241 tgaaaacaaa gccgcaccta gagctggcgc aatgatgaaa ttcgctgatt ggaccattcc
    45301 taactgtcca ttcgtccttg ttatgtgaga agatggaacg attgtcggca taatcgactg
    45361 gatcgttggc atttggaatg tttgtgcgac cgaacgcata aacaaagaca caaaaactag
    45421 ccaaatcggg aaggattctg aaatcgttcc gactactgct aaaataagcg cgaaaatcgc
    45481 tacaataata tccgtgacaa tcaaaagagc ttttttatcc cagcgatcca ctagcggtcc
    45541 tacaaatggg cttagtaaaa ccatgggaat cattcctagt aaagtagcaa aactcaaaat
    45601 tgtcgccgaa ccggtttctc ttgtcaaata ccagatgatc gcatattgga cgaccatact
    45661 agtgattcct gacaaaaact gtcccgataa aaagaacaaa taatttctct tccaattcgt
    45721 taaaacgacc gactgttcat tttccataaa catccctctt ttctttttca ttttgtatag
    45781 cgtttgcaca acaatcatta tacgggattc ttttttcaaa aaataccttt ttccctaaat
    45841 aacaatcgtt tcaatttaaa tttctttact tagaaacaat aaatcagcaa aatattaaaa
    45901 ataacaacat gtgttaaatt taaaaataaa aaagacaact aatttgcgtc gtccttacaa
    45961 agttagtcat ctaacaatag gccgtttcaa aactagttgt ctttttatat ccacagatca
    46021 ttcgactttt tcgaataacc cacggctaat catcccatcc atcagaaaat aaaatttatc
    46081 ttggctcatt ttctgttttg gatctttgaa aatattgact gctttcaatc catttgctgt
    46141 agtgatcgac attttcaatg tttgaatatc ttttgataca gtgattttta atttgaatcc
    46201 ttctttgctt tgcggcgtgg catctaatgg tcggatcaat tgataattgc ctccgtttgt
    46261 ttctgtaaat ccattatcgc gcaatgtata tctttttgca tcaggatgca aacgatagct
    46321 aattggtgcg cccagtaccg ttgctgttgt ttcaaatgcc atgattttca cctcgataaa
    46381 attagtttct ctttattata aaagtttatc ctgattttta ctaggttttt cagttaagtt
    46441 ccctaaaaaa tgaaggcgtt ctctcagaca tctgttcata taagaatgac ttcactagtt
    46501 attttttgac agcacgaact aattgatcaa ggaaatactg gatgtcgtct tcggtatttc
    46561 ccaaaccaaa gctgatacgg atggattctt ttagtatcgg tgtatcttct ccatacattg
    46621 cttccaatac gtgtgaaggc tcgatatttc ctgctgtaca agctgaacca gtagaaatag
    46681 cgaccccatt aagatcaagc ttcatcagta gcaaatcatt tgcttcgcca attaagcgaa
    46741 gattcaacac atgagggagt ttattcgtta aatcaccatt tacttgataa gggatttcgt
    46801 ttgcttctaa tgtatcaaga atcaattgct ggaatccttt atatttgttg ttacgtgctt
    46861 cacgttcttc aggtgacagc agttcaactg cttttgccat accccagatc cctgctaaat
    46921 tttctgtacc tgcacgccgc ttttcttcct gctccccgcc taaaagcaat gcagggatat
    46981 ttactccgtc tcgtttatat aagaaaccga cgccttttgg cccattgatt ttatgagcag
    47041 agatacttaa taaatcgatt cctaattctt ctggatggat cgtttggttt ccgtacgctt
    47101 gtaccgcatc tgtatggaaa taagctggat gatccgctaa aagttcaccg atttctttga
    47161 ttggcatcaa atttccgatt tcattgttcc cgtacatcac agagaccaag atcgtatctt
    47221 ctcgtaacgc ttttttgacc tcttcgatcg acagattccc tttttcatct acaggcagat
    47281 atgtaacgtc aaaccccttt gtctctaaat atttcatggt attaagaaca gcaggatgtt
    47341 cgatagctgt agtgattaga tgtctgcctt ctttttgacg cgagaacgct gtttctaaga
    47401 tagctgtatt atccccttct gttcccccac tgttaaagat gatctcgtga ggttttgctt
    47461 gcagactgtc tgcaatgatt tgtcttgctg tttccaattt ttcatgcgcg tggcgaccaa
    47521 atccatgtat gcttgaagga tttccaaacg tgtcttgcat gattgccgtc atcgcttcaa
    47581 ttacttgtgg atgcatgggg gtagttgccg catgatccag ataaatagtt ttcacttcat
    47641 tcatcccttc caaattattg ctaggcacat tctagcataa aatagcgaac aaaccaaaga
    47701 agagctcatt tttgtctaga ggacgtttca tgggcataga aaaaaggagc tgtgacaaaa
    47761 gtcttgtcgc agctccttga agctgctcac ttctgtcaca acttctttcc ctcttttttt
    47821 tgtttattcg aattcttttt cttccagacg gaataacgga ctcatcggtg tattttcatg
    47881 gatgcgtttg accgcttcac ccattaattc tgaacatgta acgattgtta agttttctgg
    47941 atgacgattt tcttctgtga aaacagaatc agtgatacaa atatctttga ttggtgcatt
    48001 gtctaaaata cttttcgcat gatcggacaa taatccatgt gacgcacagg catatacttc
    48061 ttttgcgccg ttttctttta atacatttgc tgctcgtgca agcgttgctc ctgtgttcaa
    48121 gatatcatct accatgatac atgtctttcc ttgaacatca ccgatcacat aaccaccctc
    48181 agttgaagca tcatctgcat gatctacgat ggctaatgtg ctgttcaaat attctgaaag
    48241 actacgtgca cgctgaacac cgctgttttt tggcgaaaca actacgatgt catctcccgt
    48301 cagacctaat tgacggtaat aatgagcaaa caacggcatc gtaaataaat tatctacagg
    48361 aatatcaaag aatccttgta cttgaaccgt gtgcagatct aaagtcagta ctcgagttgc
    48421 ccctgcttct tcgatcaagt tagcgatcaa tttcgcagtg atcggttcat gaggtttcgc
    48481 cgtgcggtct tgcctagcat acccatagta aggcaatacg acattgatcg ttttcgcact
    48541 tgctcgcttc attgcatcga tcatgatcag tagttccata tagtagtcat tgacaggtgc
    48601 attcgttgat tgaacgatat acacatgatc ccctcgaata ctttcttcga tattgatgga
    48661 aatctcgcca tcactaaatt gcttcacgac gcttttacct aattctgttc cgaacacttt
    48721 cgcgattttt tcagctaatg gacggttgcc attcaaacta aaaatcttca acgtgtcatc
    48781 ctgatactta ttactcatgt agtcctcccc aaaacttctc tattcttcga tagtttaaca
    48841 tatttttcca agattttctg ttaatttcag caaattctta aggaagtgaa actgttccgt
    48901 tactccaact ggcaggaatg actttatgat aatcgcccca gttgtcgtgt actttgtaat
    48961 aacgttctcc tgtttcagtt tcaaaataag cgtacgctgt cacccaatga ttgccatatg
    49021 tactgccaag tactttcaaa ataccaatca tcacaggttt gccttcacgt atacgttttg
    49081 tagctcgctg ccatgaacct agaagagttg ctcgtgcacg ctcatgattg ccattcttct
    49141 ttaaaaaact agaaatccct gtactaacct gaaaaggaat cgtaggaagt cctagtggtt
    49201 ggatctggct acgcaatgcc ttcgtcaaaa ccaacgaatc agccgtattt ttcttgcgaa
    49261 tctcatttgg aagcatccac cccttacgat agtcttgata ataggcaagg agaactgctg
    49321 ccgcgtaagt accacaaaga tagcctgagg gggtagccca tttcttatac ctcaaaaaac
    49381 gttcttctgg caatcccacc cagcaatttg cgagttcctt tcctgacctt ttttcttcga
    49441 tcaaaacggg aatatcttct tttctgttga tccatctaga taactcgatt ggctcttcat
    49501 tgatcagctt ccatttttta caagaaagac cctcttgata agtatatttt gctggagaat
    49561 attctaccgg atgtcctgat tgatcgataa tcacataact ttgatcttca aaaaccagag
    49621 ctaaaaaaag caatttactt tttcttgact gcagctgctg agtgctggct aattttttaa
    49681 tttttttatc tactttgaaa tataaaggtt tatccactac ttgattcagt tgggatcgcc
    49741 tccttttttt cattataagt gaaggattga aaaaaacaaa tcagtttgat tgttcgaatt
    49801 ttcaattttt tatgttctat ttgttaattc cttcattttg taagataatg ggcacacgaa
    49861 acaaaaaagg agcgatcgat gaatgaacaa agaacgattg aaacaagatt tctatgaagc
    49921 agtcaatgaa gaatggcaaa aaacagcaaa aatccctgca gataaaccag ctacaggcgg
    49981 tttccaagat ttagttgacg ggatcgacca acttttgatg ggagaaacag acgatctgct
    50041 tgcccatcca gaaaaagtgt cttcagaaaa aatgaaacat ttccttgcat attatcaact
    50101 ggcaaacgat tatgaccgaa gaaatgagtt gcatgctcaa ccgcttcaac ctataatcga
    50161 aaaagttgaa tcattgcagt ctttcgacga attgaataag caatttccag aatggctgtt
    50221 ggatacatta ccattgccat ttaatctgga tgtagatgca gatatgaaga atgcgcaaac
    50281 gaacgccttc tttgcttatc cgccttcttt attccttcca gataagacgt attatgcatc
    50341 agaccatccc aacggtgcac aactaatagg cgttttcttt gatatggctg ttcagttatt
    50401 agagcttgcc ggcaaagaaa aaagtcaggc agaagcaatc acggaacaag cagtgcaatt
    50461 tgataaattg attgctcctc atgtaaaaag tgcagaagaa aacgcagatt acagcaaaat
    50521 gtataatccg caatcttttg atacatttat taaatacaca gataaactgg acttgaaaaa
    50581 acttgtcacg ggtttgattg ggacaactcc agataaagtg atcgtaactg atccagtgta
    50641 cttcgatcat ttgaatgaca tcctgacaga agctcatttt ccgttattca aaagctggat
    50701 gatcattaaa acagtacgtt ctcttagtgg ttatctatca gaagaattcc gccaagtcag
    50761 cggaatctat tctcgcgtat tgtctggaac agacgaagca atgcctcaga aaaaagcagc
    50821 gtattacctt gcctctggac aattcgatca agtagttggc gattactacg gaaaaaaata
    50881 ttttggcgaa gctgctaaaa aagacgtaga acaaatggtg aaaaaaatga tcgccgttta
    50941 taaaaaacgt ctagaagaaa atgactggct tggggatgca acaaaagaaa aagccgtcat
    51001 aaaattaaat aacttaggta tccaagtagg ctaccccgat aagatcccag cattattcga
    51061 ccagttcaaa acagtacctg cagatcaggg tggtactttg ctttcaaata ctcttgtttt
    51121 cagcaaactc acattaaa
//
LOCUS       DACDLI010000012        49310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDLI010000012 DACDLI010000000
VERSION     DACDLI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.012"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(163..336)
                     /locus_tag="IV353_000745"
     CDS             complement(163..336)
                     /locus_tag="IV353_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAP9009017.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(679..1368)
                     /locus_tag="IV353_000746"
     CDS             complement(679..1368)
                     /locus_tag="IV353_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9009018.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1413..2177)
                     /locus_tag="IV353_000747"
     CDS             complement(1413..2177)
                     /locus_tag="IV353_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9009019.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2205..2903)
                     /locus_tag="IV353_000748"
     CDS             complement(2205..2903)
                     /locus_tag="IV353_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9009020.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2916..3695)
                     /locus_tag="IV353_000749"
     CDS             complement(2916..3695)
                     /locus_tag="IV353_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9009021.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(3711..4655)
                     /locus_tag="IV353_000750"
     CDS             complement(3711..4655)
                     /locus_tag="IV353_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9009022.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4696..5475)
                     /locus_tag="IV353_000751"
     CDS             complement(4696..5475)
                     /locus_tag="IV353_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009023.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            complement(5859..7934)
                     /locus_tag="IV353_000752"
     CDS             complement(5859..7934)
                     /locus_tag="IV353_000752"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9009024.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(7936..8853)
                     /gene="glyQ"
                     /locus_tag="IV353_000753"
     CDS             complement(7936..8853)
                     /gene="glyQ"
                     /locus_tag="IV353_000753"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9009025.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(9237..10049)
                     /gene="recO"
                     /locus_tag="IV353_000754"
     CDS             complement(9237..10049)
                     /gene="recO"
                     /locus_tag="IV353_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9009026.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(10192..11091)
                     /locus_tag="IV353_000755"
     CDS             complement(10192..11091)
                     /locus_tag="IV353_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9009027.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(11106..11507)
                     /locus_tag="IV353_000756"
     CDS             complement(11106..11507)
                     /locus_tag="IV353_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9009028.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(11485..11961)
                     /gene="ybeY"
                     /locus_tag="IV353_000757"
     CDS             complement(11485..11961)
                     /gene="ybeY"
                     /locus_tag="IV353_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9009029.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(11978..14170)
                     /locus_tag="IV353_000758"
     CDS             complement(11978..14170)
                     /locus_tag="IV353_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9009030.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(14194..15165)
                     /locus_tag="IV353_000759"
     CDS             complement(14194..15165)
                     /locus_tag="IV353_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9009031.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            15871..17250
                     /gene="brnQ"
                     /locus_tag="IV353_000760"
     CDS             15871..17250
                     /gene="brnQ"
                     /locus_tag="IV353_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9009032.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(17404..17850)
                     /locus_tag="IV353_000761"
     CDS             complement(17404..17850)
                     /locus_tag="IV353_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9009033.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(17877..18053)
                     /locus_tag="IV353_000762"
     CDS             complement(17877..18053)
                     /locus_tag="IV353_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9009034.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(18215..18643)
                     /locus_tag="IV353_000763"
     CDS             complement(18215..18643)
                     /locus_tag="IV353_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9009035.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            18735..19595
                     /locus_tag="IV353_000764"
     CDS             18735..19595
                     /locus_tag="IV353_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9009036.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(19687..19980)
                     /locus_tag="IV353_000765"
     CDS             complement(19687..19980)
                     /locus_tag="IV353_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9009037.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(20052..21779)
                     /locus_tag="IV353_000766"
     CDS             complement(20052..21779)
                     /locus_tag="IV353_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9009038.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EENHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(21993..22919)
                     /gene="trxB"
                     /locus_tag="IV353_000767"
     CDS             complement(21993..22919)
                     /gene="trxB"
                     /locus_tag="IV353_000767"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9009039.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(23064..24497)
                     /gene="ascB"
                     /locus_tag="IV353_000768"
     CDS             complement(23064..24497)
                     /gene="ascB"
                     /locus_tag="IV353_000768"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9009040.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(24497..25900)
                     /locus_tag="IV353_000769"
     CDS             complement(24497..25900)
                     /locus_tag="IV353_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9009041.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            26131..27033
                     /locus_tag="IV353_000770"
     CDS             26131..27033
                     /locus_tag="IV353_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9009042.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            complement(27073..28959)
                     /locus_tag="IV353_000771"
                     /pseudo
     CDS             complement(27073..28959)
                     /locus_tag="IV353_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(29052..29903)
                     /locus_tag="IV353_000772"
     CDS             complement(29052..29903)
                     /locus_tag="IV353_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9009043.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(30229..32013)
                     /locus_tag="IV353_000773"
     CDS             complement(30229..32013)
                     /locus_tag="IV353_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009044.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(32013..33764)
                     /locus_tag="IV353_000774"
     CDS             complement(32013..33764)
                     /locus_tag="IV353_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9009045.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            complement(33906..34130)
                     /locus_tag="IV353_000775"
     CDS             complement(33906..34130)
                     /locus_tag="IV353_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9009046.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            34381..35967
                     /locus_tag="IV353_000776"
     CDS             34381..35967
                     /locus_tag="IV353_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9009047.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(36033..37808)
                     /locus_tag="IV353_000777"
     CDS             complement(36033..37808)
                     /locus_tag="IV353_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9009048.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(38021..38923)
                     /locus_tag="IV353_000778"
     CDS             complement(38021..38923)
                     /locus_tag="IV353_000778"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9009049.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39202..41547
                     /locus_tag="IV353_000779"
     CDS             39202..41547
                     /locus_tag="IV353_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9009050.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(41884..42885)
                     /gene="ccpA"
                     /locus_tag="IV353_000780"
     CDS             complement(41884..42885)
                     /gene="ccpA"
                     /locus_tag="IV353_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9009051.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            43095..44201
                     /locus_tag="IV353_000781"
     CDS             43095..44201
                     /locus_tag="IV353_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9009052.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            complement(44260..44862)
                     /locus_tag="IV353_000782"
     CDS             complement(44260..44862)
                     /locus_tag="IV353_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9009053.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(44865..45305)
                     /locus_tag="IV353_000783"
     CDS             complement(44865..45305)
                     /locus_tag="IV353_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9009054.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(45430..46368)
                     /gene="galU"
                     /locus_tag="IV353_000784"
     CDS             complement(45430..46368)
                     /gene="galU"
                     /locus_tag="IV353_000784"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9009055.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(46383..47408)
                     /locus_tag="IV353_000785"
     CDS             complement(46383..47408)
                     /locus_tag="IV353_000785"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9009056.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(47452..48282)
                     /locus_tag="IV353_000786"
     CDS             complement(47452..48282)
                     /locus_tag="IV353_000786"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9009057.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(48297..49235)
                     /locus_tag="IV353_000787"
     CDS             complement(48297..49235)
                     /locus_tag="IV353_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9009058.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT        13888 a        10420 c         8768 g        16234 t
ORIGIN      
        1 atgaactata atcttcattt ctgcacagtg gataataaat tcttatagat aaaaaatagc
       61 ctcttctaaa gtcacaaata ccttactccc acattttaag taaacactct gcagcattct
      121 ctttgttata aaatgataga tattcctcaa ttttatctct tattatgtta ttatcagttg
      181 ataccattcc attaatgttt cagaataatt acaatatttt gggaccggat aactcccttt
      241 tgctcgtaac cactcatatc cgttaggatt cactaaaagt ttaccgttta tttttcgatt
      301 ctttttttga accgtcccat gaacataccg attcaacaag ctaaattata aaaattggtg
      361 tgctgtcatt attttttgga aaatttatta atggctatca atatactttt gtatcatact
      421 aaatagctcg tcagttccta tatcggatat accaatatta aagcatttag atcattatat
      481 ttaagagtgg tatcatccca aaatttcgaa tattttcttt tatataagaa taatgatact
      541 gaattgcccc attcaccttt ctttttttag attttctaca agtgattcaa accctccata
      601 attacagtta ttacttttga aacgataata tgaacatgtg taatcttttt tattaacaat
      661 ccatcattct atccctaact aatatgctcc attgggcttg acaataacat aaaacgtttt
      721 taaaatcaac ctcaagtcgt acattaaact tcgatgacta atatactcta aatccaactc
      781 aaccatttta tggaaactaa cattatttct atcactaact tgccacaaac ctgaacaacc
      841 aggttttaca tacaagcgtt gaagatcata tctcgtgtac tcagctactt ccctctccaa
      901 aggtggccgt ggaccaatta gactcatatc gccttttaaa acattccata gctgtggcaa
      961 ttcatcaatg cttttggcac ggataatttt acctatttta gtgacacggg gatcttcttt
     1021 catcttgaac attgcacctt ctatttcatt ttgatcaagt aattcttcca atttttcttc
     1081 agcatctaaa cacatcgaac ggaatttata cattttgaat gtttttccat ttttaccaac
     1141 acgtgtatgg caaaaaaata tcggacttcc tggctcttct tttctgattc gataagctac
     1201 atagaacatc aaaggagaag ctaaaatcaa accaataatg cttcctacaa tatcaatcat
     1261 tcttttaaaa aataaataga tatatcgttt ttcaactttt tctttatcca accaaacttg
     1321 attaggatgt acttcactaa tacgagataa taaatcttca ctttccatac tttccctctc
     1381 tcctcatttc ctttttatct acgaataaag aactaaaata aaccaaactt cttctttttt
     1441 acctcttcat agactggata actgatttca tccccgttga tcagatcctt tgcgacttgt
     1501 ttcatctgta ctactttttc cttaccaaag tccttagcaa tctgctcata gcactctttt
     1561 aagtaaaagg tacgcttaga gaccccatgg gcatcagaag cagccatctg cacaagatta
     1621 tgtttgacca tcattttggc tgttttctgg atgtcttttc caaatacacc gacataactt
     1681 ggtgcggtga gttgggccaa gcatcccata tctaggaatg gcagcatatg attcgggtct
     1741 ttacgaaaat gcgcgttgcg ttctggatgg acgatcactg gtgtctttcc tagttgtaac
     1801 aactcaaaaa agagctgttc tgcatataac ggcacttcca gtgtcggaaa ttcgattaga
     1861 agatatgtat catctaaatc tgtaaataaa atttcatctc ggtcgatgtc ttcgatcagt
     1921 gttccggtaa tacgtacttc ttgtccttcc aataacgtca aaggcaattg tcttttctct
     1981 aattctgctt gtaacgaagc gactagagaa atgacttgtg atttttcatt gctgtatttt
     2041 ccattattat gatggggtgt gcataaaata tgtgtgatcc cttgttgaat cgctttttca
     2101 gccatggcga tactggcatc tatgttttct gctccgtcat cgattcctgg caggatatgg
     2161 caatgtagat cgatcatttc tcttgtctcc tctcttttta tttcttaatt cccgtagtaa
     2221 tagccagcat ctttgctgtg ttctgcccca ttgtatacaa caccaaggac tcgcgcttgt
     2281 accatatcca gcaattcttt tgctttagtc aatgattctt tccgtgacat attttcacgt
     2341 acgaccaata aggtgccatc cgttttggaa gacatgatct gtgcatctgt tacggcaact
     2401 actggcggca tgtcgaagat cactacatca aatagttgtc ttgcttctgc taaaatctga
     2461 tccattcttg gcgaacttag tagttctgat ggatttggtg gtttaggtcc acttggcaga
     2521 atcgacaaat tctcgactgg tgttcgttgg atctcatctg ctacacttcc tgatgaacta
     2581 agaacggtac ttaatccgct tgcgttgttc aacttgaacg ttttatagat gacgggctta
     2641 cgtaagtctg catcgactag cagcacccgt tgaccagatt tggcaaaaac aacggcaatg
     2701 tttgctgcag tggtcgattt cccttctcca ggtcctgatg aagtcacaac aatcgtttta
     2761 atttgctgac cagctgcgga ggcaaactgg atattcgtcc gaatcgtacg atattgttca
     2821 gcgattggtg aagaggggtt tgtgagtgtg accaaactga ctgcatgagt ttgggctgct
     2881 ttttgtttct gtgttcgtgc catttttctt tcctcctaga ctcttgagcg gcttctgcgt
     2941 gatggcgctg ttttatcgtt tgtagctttt ggttgtgtcg gagctggtcc ttttttacgg
     3001 ataccagcat tcaattcttt cgctgacatt tctggtacag tccctaagat cgggaagccc
     3061 aatgtatccg tcacatattt ttcatctttt actgtccggt cgagtaattc aaataagaaa
     3121 gctagaccta cgcctaccat cattcctaac actaagccaa tcactagatt caatttatta
     3181 tttggcgaaa caggtgtcgt attggcaact gcacctgaga tgatcgagat tttatcgaca
     3241 ttcatcacgt ctttggcgtt ctcttggaat acttgtgacg ttgtattagc gatttgttga
     3301 gccgtcactg catttgtgct tgttgcttgg atcgagaaca tttgtgaatt ttgtgactga
     3361 ttgacggaga tggcatcttt gatttgtcca gcagtcattt ttacattgtc ttctgtttct
     3421 aaacgatctt tgacttcatt tagtactaga tcactgacga tcatatcctt atacgtattg
     3481 atcatttgca ggttcgtatt gacatcattg acatttgcag tatctgattg cggcagtgtc
     3541 acgatcaatt gggcttgtga gctgtatttt ggcgtcaaga tgaaaaacgt cacgatgcct
     3601 gagatggcca agccagcaaa cattgagatg aggattgtta tcacgtgttt gcgtaatgtt
     3661 tcaaaaattt ctttcaagct gattgtctct tccatttttt ctccactttc ctatttattc
     3721 tctagattca tttgatcttt taattctttt ttcacacgat caagttcttg atcgctgact
     3781 ttttggtatg agatattgtc ttgcatgaag ccttctcctt gcagctgatc ttgcttgatc
     3841 gtaccaaatg catcccgata atctaaagca atctttttca tttcatcaaa gctcatgtct
     3901 gttttcatat tcgactctag agcattcaaa acggattgat agttgcttac accgttcaga
     3961 ctcaacactt ttttcgctac accttctacg atctttcttt gacgttcttg tctgccgtag
     4021 tcaccatttg gatcttcata acgcatacga gaataagaca atgcctgatc cccgttcaat
     4081 gtgattttac cgatcgtaaa atcatagcca tcttgtgaga aggtgaggtc attattgact
     4141 tcgataccac caactgcatc aacgagttct tttagacctt tcatattgat tgaaacatag
     4201 tgatcgattg ggatgtctac atatttttgt acggtatcca tcgccatggc agctccgccg
     4261 aatgcgtaag cgtggttgat cttgtctttt gtgtcatgtc caacgatgtc gacatacgta
     4321 tcacgaggaa tgctgacaat cgttgtctgt ttgtcttcag gactgactgt ggcaaccatc
     4381 atcgtatctg atcttccttg atcgacccga cctaaatctc ccgtatcaat ccccatcaat
     4441 agaacagaga aagattcttg tttgtccaga tcaactggtt ttgttcgttc tccttgggtt
     4501 cgttccactg attcgtaagt tttttcaata gaatgtgaga gatctagata taattttgct
     4561 cctactccta cgattgctaa tactaatacg acaagaattg ctaaaataat tagaaatatc
     4621 tttttccctc ttgatcttct tcttcgtttt gccatagttt cctccgtttg ctagccttgc
     4681 ttgaattctc gcttattaat agctcgtatt gctatagccc atttgctgag cgactgtttc
     4741 tgcttttgac gcgtatgcgg ctcttatttt ttcattccgg atctgttcga tgagactgag
     4801 tgcggtcaaa tattgatcgt aagtagcagt agctgttact tgaccgtctt tcagcatcgt
     4861 atcgaatagc tgatctagtt gcgtgacttg tgcaatttgg gtatcagcga atcctagata
     4921 ttgcataacg accgttttcc aagcactatc cttaaaaaag ctcatatttt cacgtacatg
     4981 ggcgacatct gcacttgcca acttttcttt gatgatgaca tcatccacag cttgctgcca
     5041 gtttgatacg tttttagtaa ataatttatt gacagccgtt tggatcttca gttttgattc
     5101 tgcatcctct aactgtgtta agactgcttt cttttcttct tggatcgcag cagcttcttt
     5161 aggtaattct gattccttga tgtcataatc atctgctgaa agtttgatag aagatagttt
     5221 atgtgacaaa tttgaaactg tttcttctgt tatttcagga tttaggaaaa ttttttcttt
     5281 tgtactgtac agcttatcta cttcttttga taaagaggtc acggtgtctt tttgattggt
     5341 gatggcagat tctgcgtaat tgatccgttg atgatgttgc cgctgattat tgagatggta
     5401 tccaaacaag caaatcacaa tagcgcctgt gcctaccgcc acagtagcca ataacttgac
     5461 gaacggcttt tccatagatt cactcctaaa tattgatgat agataaataa agatgtgatt
     5521 cattcacatc agaatgagcg ctttttattt taaaagcata attaaactaa tatattcata
     5581 gaaatattta taaacaggtt ttccctatat aatcatacca caaacagttt tcttgactag
     5641 ggaaattcct aatttaaaag taaataaaac agtaacaaga ttattattaa tctttttcat
     5701 ttttatttcc tcacttttca tgagcgtttt gaaaattcta gcaaagaata aaataacaaa
     5761 aattattaaa atagaattaa taacagcttt tatatttata acaaaaaaac agagacacag
     5821 cacgtttcgt gcatgctctc tgtttttcaa acaaaagact attttgtgat gatttgatcc
     5881 aagcttgcta atgacaaagc catggcagtt atttgactaa gttgtgttag tcggttctta
     5941 cgtactgctg gttcatcagc catgaccatc gtttgatcaa aataattttc gatcagtgga
     6001 cgaagagcga caagctggtc gtagttttcg tggatcgttt ggtcagcaaa acttgctttc
     6061 attgcttcta ctgctttgtg taattctttc tctgcgtcgt tttcgaacag ctcagggtct
     6121 acttctgatt gtttttcaag ctgcgctttt ttcgctaagt tcacgacacg tgtcaatgct
     6181 tccattgaag gtttgaagtc ttcatctgca agatgttgtt tgaacaattg tgctgctgca
     6241 aatacttttg tcagatcttt ttgttctgca ttcaaaacag cttcgatgac atcatgacgg
     6301 atattcttcg ttgtcaataa ttggcgcaga cggcctttga taaagtcgat cacctgttgt
     6361 tgtcccgctg acaagccaat gccaaaacga tccgtatctt tactgataac ttcactaatc
     6421 gtttcttgca gaacagacaa aggaaaggcc cattttttcg attcgatgat tcgaacgaca
     6481 ccatatgctt gacggcgcaa agcatatgga tcgtttgagc cacttgggat catcccaact
     6541 gcaaagaaag aaaagatact atctaattta tcagcaatcg ctaaaacaga acctactgta
     6601 gtctcaggta aatctccttc actggatata ggcatgtaat gttcacggat cgctgttgct
     6661 acggctggtt tctcgccttt aagcagagca tatttttctc ccatgatccc ttgtagttca
     6721 gggaattctc ctaccatgtt ggttactaga tcaaatttat agatttcagc agcgcgttgc
     6781 agatccatca attcttcctc agatagtcct acttgttcgc ctatgatgcg agcgatgact
     6841 tgtacccgtt gcattttctc atagatgcta ccgatttttt catggaaagt aacagttttt
     6901 aatttttcaa cgtattggtc gattgaagct ttcttgtctt cattataaaa gaattccgca
     6961 tcttctaaac gagcagtcaa tactttttga ttgcctcgta ccacattttc caattttacc
     7021 cgatcgccat tacgagcaga aataaaatga ggaagcaaca taccttgatc gttccgtact
     7081 tcgaaataac gttgatgttc tttcatagaa gtgactagta cttcttcagg aacagataga
     7141 tatttttctt cgaagtcgcc agaaaagaca gttggatatt cgactagatt gttgacttct
     7201 tctagtaaat cttcatctag atcaatgatc cagtgatttt tttctgctaa ttcgtttgct
     7261 tgtgtgatga tcatttcttt tcttttttgc ggtgaagcaa taacaaattg ttcattcaat
     7321 ttttcttcgt attcttttgc atgcgtaaat gtcacatctt cgcctaagaa acgatgacct
     7381 cttgaaactc tgtctgtttt gacatccaag atctcaaacg ggattatttc gtcatctaac
     7441 aatgccacga tccagtgaat agggcgaata tattcaaaat cgtattctgc ccagtgcatc
     7501 gttactggga aagtgaggct tttaatgaca tctgctaaac ctgtcaatac ttcttttgct
     7561 tctttgccgt gagtgtattt cgttacatag acatattcca cgccttttaa ttctctaaaa
     7621 gtgatagcat ctgtcgtcag accttgtccg cggacaaatc cttcagctgc tttcgtccag
     7681 tttccatctg cgtccaaagc aatttttttg gctggtcctt tgacttcttc ttcgctgtct
     7741 gcttgacgtt ctggaatgtt tgttactttg attgctaagc ggcgcggtgt agaaaacgtt
     7801 tcgatcgtat cataagtcaa ttggttttca tctaaaaatt ttgctgtttt ttcttctaat
     7861 tgtttcatgc ttggtgtgac tacatgagca ggcatttctt ccagcccgat ttcaagtaat
     7921 aagtcttttg ccatcttatt cagcctcctt cacgttttct ggatttgctg tgtttttatt
     7981 caataaaggg aatcctaatt tttcgcgttc tgccacaaag attttggcta cagcacgagc
     8041 catgttgcgg atacgggaaa gataacctgc acgctctgtt acggatactg caccgcgagc
     8101 atctaataaa ttgaatgtat ggctacattt caaaatataa tcatacgctg gatgaacaag
     8161 attttcatcg atacagcgtt tagcttcttt ttcaaaacga tcaaaattgc ttagtagcag
     8221 gtcttggtcg cttacttcaa aagaatattt agaatgttca tattctggtt gtttgaagat
     8281 ctcaccatat ttcacgccat cagcccattc cagatcataa acactttcaa cttcttggat
     8341 ataagaagca agacgttcta gtccataagt gatctcagaa gtaactggtt tacaaggtag
     8401 tcctccgact tgctggaagt acgtgaattg tgtgatctcc atcccatcaa gccatacttc
     8461 ccagcccaca ccggcacaac ccatcgatgg gttctcccag ttgtcttcaa caaagcgaat
     8521 atcgtgttct aatggatcaa ttcctaaaag acgcaagctt tctaaataaa gctcttggat
     8581 attttcaggt gaaggtttca ttacgacttg aaattgatga tgctgataca aacggtttgg
     8641 gttttctcca taacgcccgt cagctggtcg gcgggatggc tctacataag cagcattcca
     8701 tggttccggt ccgattgccc gtaagaatgt atatgggctc atcgtacctg ctcctttttc
     8761 tgtatcataa gcttgcatca gcatgcagcc gtttgatgac caaaattttt gtaatgttaa
     8821 aatcatatct tggacggtca attttttact catctgtttt gcccctttca atttttaagc
     8881 gttgctgtca ataagaatac aggaaagaaa acgacaaaaa gtccctatgt ctgattacag
     8941 acatagggac gaataatttc gcggttccac cctacttccg aataaagatt cggcagcttc
     9001 atttatcgta ctcgcaagtg cctcttcaaa aatcgtttgg gtccggcttc cactctcccg
     9061 aactcgctaa accacgtgtt ttttactgat cttggtcaat gtacgtattc attttgcagt
     9121 tttctggttt gtataataga tcctctcagt gaacgatcta tttaatctac ggttaataat
     9181 agacggaagt ccctgtattt gtcaaatagt tttgattttt ttgttgtatc tttcatttat
     9241 tcttcttttt caggtatctc tttttcaggc atatttttct ctggcgtctt taatacgtgt
     9301 gcccaagact tcatttgatc aatgaacttt ttgcttttta gatgaatacc gacatattca
     9361 tcatacaact ggtcaatgag gtcacggatt gcctgcttcg tttcttcttt caagtggatt
     9421 ccactgatct tatcataaga aatagccgaa aacattcgaa cgaaataaac agcgcgagga
     9481 tctgcatgat aacggtgttc gtccattggc cagtgtttct cgcatatcac accgccatat
     9541 ttggaagaat agtcaaattt tccttttgtt tccccgcata cgacacagtg cgtccaaatt
     9601 ggcgcgattc cgaagcgttg cagtatttgc acttcaaaga tattcgtgat gatttctgca
     9661 tcatctcctt ggtccatccg ctgcagagct tgttgcgtaa attggaataa atgcgggtca
     9721 tattgatgat cttcgattgc tgcatctact agatttagca aatacgtacc ataaccgctc
     9781 aaaaagatat cttcttggat gttccgaaac gcatggacgt ctttgacact gcttaaaaac
     9841 gacaaacctt cacttcgaaa attaccgata tagactgctt cggtaaaggg gagcaaagcc
     9901 ggagctaatg gctgattttg ccgatggatt cctttgatat aaaacatcat tttgccagaa
     9961 gattccgtaa agattttgac taatttatct ttttctttgt aattacggct agataaaatc
    10021 aagcctttcg attctacaag ctggctcata ttctccctcc taaaaaataa atctggaaaa
    10081 gcagattctg ccgatccaga ttatttatgt ttcgatcttc cgctgttttt ctcaacgtac
    10141 ggatttttat ccagtttatt tctataccta acctataaga agtccagaat atcagtactc
    10201 gtcttttcgg tagccgaagt cttgcagata gacttttttg tctcgccagt ctttttggac
    10261 tttgacccaa agttctagaa atactttgtc tcctaaaaga ttttcgatat cctttcttgc
    10321 tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgattc ctttttggct
    10381 atctctttcg acgatgatcg tggcctggat atggatttta tcattctcgt ttcgcttcat
    10441 cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa acaagacttt
    10501 ctcacggatc aattcagaaa cgatgaagta ttcaggatga tcagtcactt gatcatctgg
    10561 aaaatattgt gggccctctg gcatttcact gactaataca tccattaagc gttcgacatt
    10621 gtttccttct gtagcagaga tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc
    10681 ttcgatgatt cccagcaact cgtctggatg gatcttatcg attttattga taatcagata
    10741 aacaggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc ggccacgttt
    10801 ttgatcagca ctgatcataa ataagactgc atccacttct ttcaacgcac tgtaagctgc
    10861 ttctaccata aaatctccta gacgatgctt tggtttatgg attcctggtg tatcgataaa
    10921 aatcagctga gcttctggtg tagtatatac tccttggatt ttatttctag ttgtctgtgc
    10981 cttgtcactc ataatggcaa ttttttgacc cacgattcgg ttcaataatg tggattttcc
    11041 tacattcggg cggccgacga ttgcgacaaa ccctgattta tgttctggca tatacgttct
    11101 cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa cccaatgatg
    11161 acagcaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat ttttttccca
    11221 atcggatgaa aatgaaaatc cgtaaacatg tccactacat tttcaaaaac agtattgatg
    11281 atctctacta tccaaacgag aaaaacagct aaaagcagcc ataaccactc tatccgatca
    11341 agctggaaaa tcacgcccat aaataaagca aggataccga acgcaacatg tttcctcata
    11401 ttgcgttctt cctcaaaaac ggttttgact ccttggacag caaattccac agacatcagg
    11461 aaatgtttgt ttttctctac gtttttatct tttgagtcca taggcatcca agatctcttt
    11521 ctgtaaacca aacatttctt tctcatcttc tggtgtcata tggtcatacc cgttgatatg
    11581 caaaaatcca tgtaccgcta aaaagccaag ttcccgatca tatgaatggc cgtaagcttc
    11641 tgcttgttca gcagcacgtt caatagagat catgatgtct cctagtacac gaggtaatgg
    11701 attctctcca tcttcaaaaa tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa
    11761 actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta cttgaattgc
    11821 cgcattgtcc atgaaagtaa cagacatttc tgtttcctct ggcagcttta aataagatgc
    11881 tgcaaattgc agcaagtcat cgatttcttt cattttctct tctgagactt ggtttgtttc
    11941 atccataaag gtaatatcca ttatacatcg cttctttcta ttcttcttgt acttccttta
    12001 tcttctcaac ttctgttttc attttaggat atttgatccg agaatggaag gtgctactta
    12061 aaccgctgat taatgatttt tccacttttc ggatttcttt caaggtcagt ccactatcgt
    12121 ctaattgacc atcggaaata cgctcttcga taagattatg gacaaaactt tcgatttttt
    12181 cgctcgtcgg atgatccatt gcccgtactg cagcctcaca actgtcagca atattgacga
    12241 tccctgcttc acgactttgc ggttttggtc caggataacg gaattgctct tctgttactt
    12301 ccggattgcg ttctttcgct ttgatgtaga aataacgcat tagcgtggtt ccatgatgct
    12361 gtttgcaaat atcgatcacc atttgcggca tattataatc ttccaatatt ttggctccat
    12421 ctgtcacatg tccaaagata atatgtttac tgtcttctgg caataagaaa ttgtgcgggt
    12481 tttccgctcc tgaaggcaag ttttcaacga aaaagctagc atgtttgatc ttgccgatat
    12541 catgataata gcaagctact cgagttagca aagaacgtcc gccgatttct gctacagcgt
    12601 tagcacttag attagctacc atcatactat gatgataagt acctggcgct tcttccagca
    12661 actgtttcaa taaaggatga tttggattgc tgagttcatt taatacaatt acactgtcat
    12721 cattgaccat caattcaata tatgggtgta gaccgatcgt cagcaagaat gagaagagtg
    12781 caccagtgaa cccgcagacg agcattaacc aagtcgttgc atcaccaaag ctcattcctt
    12841 gatagacgat caaaatgaca tccatcatca caggaaaaat aatgacccac atggcagcag
    12901 ataatccttg ttctgagatc cgtttgcgtc tgacaatcgt tgccatgagc ccagagaaaa
    12961 cataagacat caagataatc gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa
    13021 gtgcggatac tgcttggaaa ataccagcca tgatccctgc tcttcgattc aagaaaaagc
    13081 ttaaaacaag tggtgcaaaa gcagcgggat aaaacagcgg gatataagta gcttgttccg
    13141 tttggaataa ttggaaaaat ttcatcagca ctatgctgac tgacatagct gtcacataga
    13201 aaagcacgta ttttgttctt tctcctgcat tttggaattg aagtgaatta taaatcagta
    13261 cgccgatttg aagcaaaaca gctaaaacca tggcaattag cggaaaaatc gaagttgttt
    13321 gattagtcag tcctagtaat tccaattttt tcatagctga agcatcgatt tgattacctt
    13381 cacgaacaat gatttctcct tggaagatca tcactggttg tacagcttct cgtgcggatt
    13441 gtttcaattc ttctgtcttt ttctcattca atacgtcatt cactacgata ccttgatcca
    13501 ctaagtagcg aatcatttgt tgttgtgctg gtgtgacatt caaatattgg atttgctctt
    13561 ctgcttcttg tttaaaagct tccaattcac tttcacgaac ttgtttgctc atctgttcgt
    13621 caatgagctt cagactctca tccctcacag tatgaatctc agtttcagtc atttcaaata
    13681 tcgtcgaata aaaattgttc ggtagttttt gataaaacac aacattttct gcattcacat
    13741 tttcaaaatt tttcttcagc gcggcgatcc gctcttctgt agtaggcttt gggatatttt
    13801 catcttcctt tgccttatca atcttttcgt cataagattt gttgatggca tcattcgtct
    13861 tatcgatcaa ttcaaatagt tgcttgatcc gattgttctg gtcatctgca agatcttttt
    13921 ggtaagtgta ttctggagtg actgcttctg ctgccagttt tcttttttgt tcggtttctt
    13981 ccgtgttctc gatcgttttg ttcgcacgga tactttcttc tgctacttgg ccttctctga
    14041 aatcattggc cttttgtttg acactactaa atgtaacgcc gcagataatg ataaaaaaca
    14101 agaacagcag gaaaggcaat gctgcctttc caagtttttc ttgtagtttc tgcagtgttt
    14161 tattcaacat gtgcttatcc tcctaagcaa agcttattct tgatggagat ctgccttttc
    14221 ataagctttg atgatttcgg caactacagg gtgccgaaca acgtcacttg cttcaaaatt
    14281 aacaaaatcg attttcttga tttgctttaa tgttcgttct gcatgaacaa gaccacttgt
    14341 cgtccctcga ggcaagtcta tctgacttgt atccccattg acaatcattt ttgattgata
    14401 accgagacgg gtcaagaaca ttttcatctg agcgactgtc gtgttttgtg cttcgtccaa
    14461 gataacgaat gcatcatcca atgttcgccc ccgcatgtaa gctaaaggtg cgatttcgat
    14521 tacgcctcgt tccatcaatc ggttcgtatg atccaatcca aaaatttggt ataaagcatc
    14581 ataaacaggc cgcagataag gatccacttt ttcttttaga tccccgggaa gaaagcctag
    14641 attttcacct gcttccacag caggccgcgt caaaatgatt ttttgcactt cacctttttt
    14701 caaggcagcg attgctaata ctactgcaag aaaagttttt cctgtcccgg ctggtcctac
    14761 gccaaatacg atatcatgtt tggctaccga ttcgacatat ttcttttggc cgctgttttt
    14821 tacccgtata ggttttccat tacggtcttt gacgatctct tcctcataca tctcaacaaa
    14881 ataatctaat gtaccttttt gccccatttt caatgccgtc acaacatcgg gcgtacttac
    14941 gtggattcct cgtttgatca attcctgtaa tgtccgaatg actgaggcag ccaaggaaat
    15001 ctgggattgc tcaccaatga tctgaatgat ttctccacgt gtatggatgg ttgtttccgt
    15061 tgtttcctca atcagtttta tatgcttgtc gtgtgcacct aaaagcatgc tgacatcatc
    15121 agcagccgtc aatctaatct ctaaagaact agactcttcc gtcaaaaata gacatcccct
    15181 ttttttcgat taattctttc ctattttacc atactcagaa gaaactatat agcaaataag
    15241 aaaaggttct aaaagagttg cttaaccata agtaccaaga aacgttaaaa aactagccgc
    15301 ttccattctc taaaggtctc gtttgaataa aaaaagctga tcatgtaagc tatgcataag
    15361 atagaggaca agtattccgt ctaacttagc aagtattctc tgggtatttt tcttgtttca
    15421 aaaaaagaaa acaaaatatt tgaaatattc ttgcatttca aaaaaaacag gtgtattata
    15481 acgtcaacaa ataaatatac agcaaagcgt cgaggaaaac agtaggctat tcagtgattt
    15541 attcccagtg agtcatgtaa gtgagaaatg acaataaatc gaatagacga acatcatttc
    15601 cgagtcaatg tcgaaaacgt agatcattgt ggctgcctcc attatcaggc tagcggataa
    15661 ctttatcgaa aagcgtccgt gaatgaagaa gcttttttaa gcgaaaaaag gtggtaccgt
    15721 tctagaatct agagcccttt tcccaagtgg aaaagggctt tttttatatt ttaaggtggt
    15781 ggttttcatg gaatctgata ttagtggtat ttctagtttc tgcaacgttt attttgttac
    15841 tcaaaatgat aattattgga ggaaaaattt atggaagaaa caaaaactta ttcatggaag
    15901 caattattga tggtcagttc acttatcttc gggatgttct tcgggtcagg taatctgatc
    15961 tttcctgtac acttaggtca aatggctgga tcaaactgga tcagcgctgg tgtaggattt
    16021 gccatctcag gcgctctttt cccattatta gctatcttag ctgtagtagt gacaaaaagt
    16081 gatggtctgt atgacttagc taaacctgtc ggaaaatggt atgctgcctt gttcttgatc
    16141 cttgttcatt taacgatcgg accattcttt ggtacaccgc ggacagcagc aactgctttt
    16201 gaaatggctg ctaaaccctt tttgcctgaa aaatatgatt cattaggtat gtttttattc
    16261 tctgctcttt tctttttact tgcttatttc cttactgtac atccaacacg tttggtcaaa
    16321 tatgtcggaa agtatttaaa ctccttgttt cttgcattat tagctatcat ttttgtcgtt
    16381 tctttattcc aacccatggg gaatctaaac caatctgctg cagattctta tcagacgaac
    16441 gctttgacaa atggcgtttt agaaggatac aacacggttg atgcagtagc tttacttgct
    16501 ttaagtgtca cttttgtcca tgcagttcgt gatcttggtt ttaaagataa aaaagtcaca
    16561 gaattaacag caaaagctgg tagcctgagc gttgcgttag aagtcatgat ttattttggt
    16621 ctggtccttc tcggtgcgat gagtttgaat aagatgtctt tatcagaaaa tggcggaact
    16681 gcactttctc agatcactaa tttttattta ggtaacttcg gtgctagctt cttaggggta
    16741 atggtcacat taggtgtctt cactactgct atgggactcg ttgtttcttt tgcacaagat
    16801 ttccataaac tgttccctaa agtcagttat atgacatggc tgcggttaac gacttttgtc
    16861 tcttttgtcg ttgcgaatgc cggattggat aatatcatcc aatggtcttt accagtattg
    16921 atgcttttgt atccattgtc acttgcgtta atcttacttt ccttaacagc aaaattcttc
    16981 caaaagagtc catttgtcta tcaagtaacg atgttctttt ctgccgttcc agccgtattg
    17041 gatatgttgg caaattcgcc tgcattagtg agtcagcaaa ctgtcgtagc aagcttacta
    17101 ggattttatc atcattatgt tccttttgct gcattaggtc ttggctgggt cgtcccaaca
    17161 ttgcttggtt acgctggcag cttgctcttt tattatgtat atcgcgtatc aggatataaa
    17221 caagaagcaa gtgagttacc agaagaataa ccaaatcaaa cacaatttaa cataagagat
    17281 attaaacagg aaaaaaacag ctagaagaaa gaataagatt ctttcttcta gctgtttttt
    17341 agttcttttt ttacgaacac aaaaaactgc ggataatcac tgtccgcagt ttaaatgata
    17401 aaattaagat tgtaatagtt ctttcacaat cgcattcact tggtttccat ctgcttttcc
    17461 tttgacttta ggcataattg cacccataac tttgccaaat tctttcattg atgaagcacc
    17521 agtttgagtg attgcttctt gaacaagttg tcgaatttct tcgtcggaaa gttgttccgg
    17581 catatatttt tcgactatta caatttcact ttttactttt tcagctaaat cgtcacgtcc
    17641 tgcttcttca aactcatgca aagaatcacg tcgctgtttc atttcacgag acaacaccgt
    17701 tagttcttct tcttctgtca agtcgtgacc ttctttaatc tgctcgtttt gtatagatga
    17761 cttgatcatt cggatgactt gtaaagattc cttatctttc gctttcatcg ctgctttcat
    17821 gtcatcgttt aatctactta gtagtgacac actaccaact ccaattcatc acagaactag
    17881 aattttttgc gttttctagc tgcttcagat tttttcttgc gtcttacact tggtttttcg
    17941 tagaattcac gtttacgaga ttcttgtaaa gtacccgctt ttgacacgga acgtttgaag
    18001 cgacgaagag catcgtcaag tgattcgttt ttgcgaacga ctgtttttga catgttaatt
    18061 ccctccctcc gagcttaaaa agtaaccgta attgttattg aaatatatct caaaaaacaa
    18121 gactgtcctg taaatattat atctaatgct gttttgaccg tcaaggaaat cttgcttttt
    18181 tttaaaatta tcaaatgtta ttttatttaa atccttattg acaattttca catcttccga
    18241 aaatctcgaa tctatgccct tcgatcgtgc atccacctag ctgttcttcg aaataattca
    18301 tcggacacat atggatttct ttcgttgttc cgcacactgt acagataaaa tgatggtgat
    18361 ggccgatttt cccctgacag caatgaaaac gaaacttcat ttctccattt aaatcggttt
    18421 cttccagtaa atcgatctct gcaaaatcat ggagattccg gtaaatcgta tcataactta
    18481 cccctttgaa ttcctgattc atatagtcaa aaacttcttt agcagatacg tagcggttac
    18541 gcttcactaa ataagttaac aatgcttctc tttttttagt atatttaaaa ccgttatttt
    18601 tcaatgtttc taatgattgt tcgatcaatg tctgtttttt catgacttat ccctccaaat
    18661 caaaatgatt acgaagtacg cattctagag tatggtataa taaacataaa aaaaatgcaa
    18721 gtgaggtttt atccatgact tccgattcta cagaaatcgt tacttttttt gtcatctccg
    18781 actctgctgg agaaactgcg acaaaactag cgcaagctac tatggctcag tatcctaccg
    18841 ttgagttcaa cctattccgc agaacgttcg tgacagatga aaaaacatta aggcaggcat
    18901 tgcaagatgc attagaagaa aaagcacttg tcctgcatac attaatcaat caagaactga
    18961 tcgagataac acgttctttt tgtgaagaac atcaattatt tagttttgat gtgatgacgc
    19021 caccgatatc agaaattgaa cgtttaactg gtatcaagcc aattcggcaa cctggtgccc
    19081 tccatttatt gaatgagaac tatttcaaac ggatcaaagc aatggaattt gctgtaaaat
    19141 acgatgatgg gaaagatcct cgtggttttt tagaagcaga tgtcgttttg ctaggcgtat
    19201 cccgaacttc caaaacgcct ttgagcttgt ttttagctaa caaaaattta aaagttgcca
    19261 atttaccttt gatcccacaa gcacatattc ccaaacagct ttgggagatc gatcctaaaa
    19321 aaatcgtggg actaacaaat aacccagata ttttaaataa tatccgtaaa gaacggatgc
    19381 gctcttacgg attaaaccct gatactgctt attccgatat cgaaaaaatt agagctgaac
    19441 tcgattttgc gaatgatctt tatgaaaaac taggatgtgt agtgatcaat gtagcatctt
    19501 tatctattga agaaacggct tcgcttattt tgaatgcttt agacctagaa gatcatagtt
    19561 attatggaac tgaaacaaca gaaaaaagcg attgatcatt tttgtctttt tcttaaaaaa
    19621 cactggagtg tgacaacgaa atttcttcgt tgtcacactc cagtgtttat ctatttgcac
    19681 ctatcgttat tgatgttcca catgtttgag tgtctggttc aaaatatcca gtacatgaga
    19741 atcatcaaga ctatacaaaa tcgcttttcc ctcgcgatga gacttgacga catgattttc
    19801 tcttaataaa cgtagttgat gcgatacagc agactgctcc atttccactg cttgggtgat
    19861 tgctgtcaca ttttgttccc cgtctttcag atatagcaga attttaagac gagtgggatc
    19921 actcaacatt ttgaataatt gactgacttt ctctactttt gatttttgcg tgtctggcaa
    19981 tccattcacc tccaacttga aaaaagactg acttctaaag tttaccactt tttccatcag
    20041 tctcaagttt tttattcttc tgtcatcttt ttgataatcg cttcataaga ggctactttt
    20101 tcggctgcgt cttctgtaga acttcctttt acaccaatat aaaacttgat tttcggctca
    20161 gttcctgaag ggcggatagc aatccagcca ttttcttcca atgtatattt caatacgtcg
    20221 gatggcggtg ttgtgagttt ttctacgttt ccttcacggt ctgttctcgt caattcttta
    20281 aagtcttctg tttgaataac ttttgctccg gcaaattctg tcggtgcttc tttacggaat
    20341 ttttccatca aagcagagat tttcgcagaa ccttcttgtc cgctcattgt cacagaaatc
    20401 gttttttctg cgaaatagcc ataggtttca aaaatcgttt gcaagccgtc atacaacgtt
    20461 ttgccttgtt ttttatagta agctgctact tctgctaata aaagcaatgc ttggatcgcg
    20521 tctttatcgc gaacaaaagg tttaactaaa taaccataac tttcttcgaa tccaaacata
    20581 aaagtatgtg aatggttttc ttcgaattgt tggatttttt cagcaatgaa tttaaaccct
    20641 gtcaacacat tgaacatggt agcaccgtag tgttccgcaa ttgctgtagg caattcgctg
    20701 gaaacgattg atttcaatac cgctgcgttt tctggtaaag tgcctcttga ttgatgtgct
    20761 tccaaaatat attgagcaaa caatgctccg atttggttcc cagtcaatac ttcataagaa
    20821 ccatctggca aacgaaccgc tgcgcctaaa cggtctgcat caggatctgt tgcaatcagc
    20881 aagtcagctc cttctttttc tccaagtcgt atagcatatt caaaagctga atgttcttct
    20941 ggatttggcg atttgaccgt agtgaaatct ggatctgcca cagcttgttc gggaactagt
    21001 acgaattgtt cgaatccagc ttgtttcaac gcacgttcgc ccaacatttt ccctgttcca
    21061 tgaagtggcg tgtacacaag tttcaaatct ttccccattg tttcaaccaa ttcacgatca
    21121 atcgtcactg ctttgatttc ttctagataa gcagcgtcca cttcttctcc gatgatcgtg
    21181 atcaacccgc tatgtttagc ttcttcgtca gataatactt cgacttttag cggattgctg
    21241 acttcacgga catatttcgt caatgcatct gcgtctgcag gaggcatttg tccgccatct
    21301 tctccgtaga ctttatatcc attgtaagcc gctgggttgt gagatgcggt taccatgatt
    21361 cccgcaaacg tcttcaaatg acgaactgca aaagaaagct ctggtgttgg acgcagactt
    21421 tcgaatacat aagacgggat attgtgatgt gccagtgttt tggctgcttc cattgcaaat
    21481 tctggtgact gatggcgtga gtcataagcg atagcgacac ctcggcgttt tgtttcttca
    21541 ccttgtgtat ccatgaaacg tgctagtcct tctgttgctt gtcttaccgt atagatgttc
    21601 attcgattga tcccaggtcc taagattccc cgcatgcctg cagtaccgaa ttccaacggt
    21661 gcataaaaag catcttcaag tttttcagga tcttcaccca gatcatttaa ttgttttttt
    21721 aagttttctt ctaaattttc ttcatgtacc cattgttcat acacttgttc ccaagacatg
    21781 aaaataacac ctctttctga aatatggttc ttaataagta tagcattcac ttattagata
    21841 tgaaagtatg aggaccaaaa aacgcttaaa tcaagaaagg attcttttat caagcatgat
    21901 tgcaagtatt cacataaatc ggattcagtg gatttcacgc gaaaaaaaag agtaaaagaa
    21961 caattgaaac cgttctttca ctctttttaa acttatgctg ttgtttcttc taaagactcc
    22021 aagtatttat acacttgttg tccagcaata ccgccgtcac caacagctgt agtgatttgt
    22081 cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc ctggcatatt cgttttcatt
    22141 tcgttgtctg ttggaatcca gccagcttcg ttcgtcaaac ctgcttctct aaaaggtgca
    22201 gttaatggat ccagtccaac atagatgaat actccgtctg ctgctaaatc aaattcattg
    22261 ccggttttga cattacgtgc tttcacacct gtcactacca tttcgtttcc aacgatttcg
    22321 tcaacgactg tatcccacat aaaggaaatt ttttcattcg caaatgcacg gtcttggatg
    22381 atcttttgtg cacgtaattc atctctacgg tggacgatga ctacttcaga agcaaattgt
    22441 gtcaaataga tggcttcttc tactgctgag tctcctcctc cgatcactac tagacgtttg
    22501 ttacggaaaa acgcaccatc acaaactgca cagtaagaaa cgccacgtcc agcaaattct
    22561 tcctcgccag gtacgcctaa tttacggtgg atacagcctg ttgcgataat gactgttttt
    22621 ccttcgtaag ttttatcttc acaaataact tctttgtggc tgccgtgatc cttgatatct
    22681 tgcacgattc cataagcatt ttctgtccca aatcttgaaa caccttcata catcttataa
    22741 gctagatcag gacccatgat tgaatcgaaa ccaggatagt tttcgatttc tgccgtatta
    22801 ttcatttggc cgccaggggc gccttgttcg atcatcaata cggataagtt tgaacgtgaa
    22861 gcatataaag cagcagtcat cccagcagga cctgcgccaa taacgattac atcatacata
    22921 tgtagattct cctttactta atcaagtaag catactttac agccaatgcg aaaaaaagtc
    22981 tattgttctg cttgcgtttc tcgaatagct ttattatact cgaaaaatcc gctgaaaaac
    23041 aatagacttt tataaatcat tgattaatca ttcgaaagat tttctccatt tgaatcaatt
    23101 acttttttat accagttgaa tgatttcttc ttcgaacggg ctaatgtgcc atttccttcg
    23161 ttgtcacgat cgacataaat gaatccgtac cttttcttca tctcacctgt accagctgat
    23221 actaaatcaa tacatcccca agtcgtatag cctaatagat cgactccatc taattctact
    23281 gcatctttca ttgcctggat atgtgctgcc agatagtcga ttcgatagtc atcttccaca
    23341 taaccattct catcgggcgt atcgactgca cctaagccgt tttctacaat aaatagtggt
    23401 ttttgataac gatcataaat atcattcatc gttattctca agcctaatgg atcaatttgc
    23461 caaccccatt cacttgcttc cagatatgga tttttgacag aagcaaagat atttcctgca
    23521 gtctgttcca agagttcgtt gtcagtagtc gaaactcgag aagaatagta agaaaaggag
    23581 ataaaatcaa cagtatgttc tttcagcaat gcggcatccc caggttccat ttcgatctga
    23641 atgccatttc gttccatttc tttcaatgca taagcgggat attctcctcg tgactggaca
    23701 tcaatgaaga aataattttc tcgatctgat tttctagaag cccaaacatc ttcaggattg
    23761 caagtgtatg gataatatgc tcctgctgcc agcatacagc ctactttgtt ctccggatct
    23821 acttcatgag caatcttcgt tgcgatggca cttgcaacca actcatgatg tgccgcttga
    23881 tatttgattt gttcttcatt ttctccttct tcaaaataca gccctgctcc catgaatggt
    23941 gcatgaagga tcatattgat ctcattgaat gtcagccaat atttgacaag tcctttatag
    24001 cgattgaaga tgactcgaca cagattttca taaaaaccaa cgagttctct ggaacgccac
    24061 gcaccatatt ctttgatcaa atgcattgga caatcaaaat gcgtaatcgt cacaagtggc
    24121 tcaattccgt gcttttggca ttctttaaag gcatcctcat aaaatttcaa gccagcttca
    24181 ttcggctctt tctcatcccc cattggaaaa atacgactcc aggcgatgga cattcgataa
    24241 gtcttgaacc ccatttctgc aaacaaagca atatcttctt tataacgatg atacatatcg
    24301 attgcaattt ttgctggata aaaatgatcc tcatcaaatt caaacatttt tttctttccg
    24361 gtgataacag gaaaacggtc ttttccgatt ggcacaacat ctacgtttgc tagtccgcgt
    24421 ccaccttcat cataaccgcc ctcacattga ttggctgctg tagcaccgcc ccataaaaaa
    24481 tcttttctga atcccattat acttcctcca ttatatgtgt attacctgat aattctcttg
    24541 ccagttcatc ttgaacttcg attccttctt cttttaatcg gctgattgtc tccttgaatt
    24601 gcggtaaata ctgctggtgt gcaataaaca actcatccat gatccgtttt gctgtctttc
    24661 ctgatcgtat aagtggattg atcgtaaacg cctgtaaagc tgtcccataa tcaccagtca
    24721 cagctgcttc gatcgtcagc aattccatat tcttcattac ttgcaaccag cctctttcag
    24781 cagcaggaag agaaccaaac gctacatttc tcgctccttg tgcgccaaca taggcagtga
    24841 cttctactac acaatctgcc ggcaggtcag gaacagctcc atcattcttt gtagatacca
    24901 cgatctgtgt ttctttattt ccatagattg aagcaatcgt ttcacaggca gcgtctgaat
    24961 aataagcgcc acctcgtttt tgcaattgtt caggtttatg gtctaattct ggatctttgt
    25021 ataattcaaa tagctctgct tctgtttctt tcacttgctg cgctcttgta ccaatcgttt
    25081 gatattcttc taaagcatgt tccagcattt cttcttcacg atagtaatag cggtggtagc
    25141 cacaagggat catgttcatt tgttttaatt gttctttgaa aaacgggata tcaaagatat
    25201 ttttcggtaa tccattctct tcatcgtata aacgatcaat caattctgcc gtgcggtcat
    25261 ttcctttatt atctgctact ttgtgccaat ggaaatgatt tagaccggca aacttgtaga
    25321 tcagctcttc ttttttcttg ccaagcattg ctggttctgt catcatcgcc atcactggca
    25381 cattgcataa tccaatcact ttatcccatt tgccatatct gataactgct tctgtcacca
    25441 tcccacttgg gttcgcgaaa ttgatcagcc aagcatttgg acataaacgc ttcatgtcct
    25501 caacgattgc caaaacgact ggaacggtac gaaaagcttt gaatatcccg ccagcaccat
    25561 ttgtttcttg cccaatcatc ccataataac tagggatccg ctcgtctttg attcgtgcat
    25621 ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc atctttcaat gcttcttccc
    25681 gatccagagt tgcatagatc ttgacttcat acggagaagc atcccacata cgctgagaca
    25741 tctgactaac gatctcaagc ttttcttttc cttcttctac atctacgagc cagatttctc
    25801 gtatcggtaa ttcctcatat cttttgataa atccttccat caactcgggg gtgtaactgc
    25861 tgcctccacc aatcgtagcg attttgattc cttttgccat gttctcactc ctttactttt
    25921 ttacactttc attatctttt ttgtaaacaa aacaaacaat agaaatctgt ttctttccag
    25981 taacatttac aaaatttact gttttctagt ggatttttgt aaatatcttg tcaaaaaatg
    26041 tctattttaa gttatatagt ataaatccgt ttacaaatca ttttcaaaaa agttataatt
    26101 tacgtaaaaa aataagaaga aggtatctac atgttattac gtgaaaaaat caaagaaatc
    26161 tccttttctc cttcagaaaa tgaagtgatc cagtttctct taagcgattc tccagcactt
    26221 ggcgaaatga ccatccaagc gattgctgag cgatgttatg tccacccctc tacactcatc
    26281 cgtgtagcaa aaaaaatggg ttttgatggc tgggtcagtt ttcgtaatgc ttttttagct
    26341 gaaacagcct atcttgaagg gaattttcaa gacgtagatg cgaatcttcc tttttccacc
    26401 acagacggta ttatgacgat cgcaaataaa atggcttctt tagaacagat gacgattcat
    26461 gatacacttt ctctcattca tcatgaccaa ctgcaaaaag ccaaacaact aatgcttcaa
    26521 gcaaaaaaaa tcgtgatttt cgccagtaac tcgaatactt tgatttctca agatttcatg
    26581 ctgaaaatga agcgcatcaa aatggatgtc acgattgtca ccacttttgg tgaatcattc
    26641 tatgaagcgt acaattgcac gagtgatatg tgcgggattc tgatttctta tacgggtgaa
    26701 aacaatgtga tccttcgtac cgcagaaata ttgaaacaaa atcatgttcc aattttagct
    26761 ttgaccagta tcggtgattc aacgctatct gcaatgagta cagccgcttt gcgacttacg
    26821 acacgcgagc gactgtattc aaaaattggt aattttacga tcaatacatc aatttgttat
    26881 cttctagacg tgttatatag ttgtgttttt gcagaaaatt atcaacagaa tttagtccat
    26941 ctaatttctg tcggtcaaaa attcgataaa cgccctatca gctcagccat catgaaagag
    27001 cctttcaatt ttgaaaataa agaagtggaa taaaaagaat agttaagcct agtccattct
    27061 tcacctaacc gttcaggtca gtgcggtgat aagtgcatcg tctggcccga ccgtctcccc
    27121 ttggttttcc aaaatatcca aataatccgt gtaattggtg acgatcacag gtgtttctaa
    27181 actgtatcct tccttttcga ttttcttttt gtcgaagcgt agcaacagat cgccttgttt
    27241 cactctttat ccttgctgca ctaagctttc aaaatatttc ccttccaact gcacagtatc
    27301 caatccgaca tgaatcagca attccagccc tgtttcagaa accattccaa ttgcctgttt
    27361 cgtcgggaac agggtcatga ctgttccgtc aaaaggggca cgtacttctc ctttttcagg
    27421 gtagatcaag attcctcgac ctagtgttcc ttgtgcaaaa gctgcatcct ttgctgcatt
    27481 tagtggagcg atttgtcctt gtatcggtgt ttttacaatt tctttacctg attttttctg
    27541 tatatcatct gtttcacctg cttcatcctt ccaaaagaaa aaagtcagcg taaaggatac
    27601 caccatcgca atggcgatcc caatcaatac attaatcaca tctgttaaat caccatctgg
    27661 tctaatatag ttgggaattg taaagatgcc tagtccacct tgattccaag aagttaaatt
    27721 cattaaccca ctatacgcgc cccctaacgc accaccgaca caggtaaaaa taaatatttt
    27781 tttacgtggt aatgtaaacc cataaatagc tggctctgta atccccacaa taccagaaac
    27841 aatggctggt attgcaattg ctttgtcttt tggatttcta agcttaaagt agagtgcggt
    27901 aagtgctgca gtttgtccaa aagacactac tgcaacaggt gtcataatat tttgccaacc
    27961 attcacagaa aattgcatca atcccattgg tacgattgcc caatggagac caaacatcac
    28021 cagaatttgc cagaagaagc ctacgattgc tccataaata actggcgaaa gtgctaaaag
    28081 tgtttcaaat ccagccatta aaatatcagt tgcgaatgtg aatactggtc caacgagtaa
    28141 aaaacctaaa ggcattgtaa taagaacggt gaagaatgga acgaagaaat tcgcgatcat
    28201 ctcaggtact aactttttaa ttggtttctg aatttggcta gctaacaaca caataaagat
    28261 aatgggaatg acagaagaag cgtagttacg cgcaatccag ggaatgccga atactgtttg
    28321 ataaataggt gcttcaaaaa tagtgccaga aaatatggtc gttagtggtg cttctgctgc
    28381 accactcaaa gcttccagcg acaagctagg attcattaat gctgcgccaa gcaacatccc
    28441 tacgaattca tttactttaa atttacgggc agctgttaaa ccgataataa caggcataaa
    28501 ttggaataca gaatcaccga tattatcaaa cataacaaat gtgccagaac cgcggtcaaa
    28561 cgcgcctaag aagacaagga atgctgtcag tccacgaatc atcccgccag cagctaggat
    28621 agcaaggaat ggctggaaac agcccgatat tccatcaata agcatatcaa acggtctcat
    28681 tttttctgtt gaaacagcct catcaaaagt aaggtttcct gcttttataa cctcttcaaa
    28741 cacgactggc acatgattcc caatgacaac ttgatactgc cctcccgctc gcattaccgt
    28801 tacaataccg tccatatttt tcaagatctc atcatttgct ttttcttcgt tttttaattt
    28861 gaaacgaagc cttgtgatac aatgtgtcaa accattgata ttttccttcc cgccgacatt
    28921 ttctacgatt tttctcgcta attcttggta ttttcccatt tctattccct ccaatttatt
    28981 agtggaaggt ttagccaact gaatgtcact acccttattt catacgttct tgttaattta
    29041 tcctcctttt tttatttaca acgattctca tgcactaaac gcgcagtatg gatcgttaaa
    29101 tacatttttt cgtccgtgga catctggtag ttgaacgtct cggctaaaaa gtctgctatt
    29161 ttctcaacgc aaccaaacgt atccttatat tttttttgaa taacctgaaa caattcctca
    29221 gaaacttctc ccacttctga tttttctcca tctaggacgc gtgtcagaaa aaaacgtaag
    29281 tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa atgtaatctt atagtaatag
    29341 cggacgatat ttagaatatc ctgtattgtt atagtaaaca ggtaggtatt gtcttgctgg
    29401 ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa atccggcttc atcttctgat
    29461 aacttgatgt ggtatttatc cgagattttc ttcactgttt ttttcccgat ggcatactct
    29521 ttaggaaata gttgtttgat atcatacagc aatacatttg gtacaaatat tcccattttg
    29581 tgacgttcga tagccgtata caaatgatcc gtcaatgcaa tatacacaga atcattcaac
    29641 tggacttgta gagcctcttt tgtatattca atgatttcac aagaaatttc taagtactca
    29701 agaggaatcg cttgtgccaa ttcttggaac tgttgtgaaa gcccgtcatt tgtcaactga
    29761 aattttttgt cgatgcatga gtcaaaaatc gggtcaccca ctttcttttt gaatgccaaa
    29821 ccttttccca tcacgatcat ttcctgattt ctttcatccc tggttattac gacgttgtta
    29881 ttcagtactt tctcaatcag catgttttgc tcctttccat gatgtatagg gctgtatcca
    29941 aaacaaaaag aaaaacccaa aaagccgtcc atgaaaccat gggagactca tcaggtttag
    30001 cttgcatcag gcaatcacta tccaagttgt atcgttttct ttatattatc gaaagcaaat
    30061 gcttttgtca acgctttctt agtggctatt aggcacttct gactagctgt cgaatctttt
    30121 tttaaatctg gtgactacga cactaactct cacagatgaa aaatatgaga agctatgttt
    30181 tattttcact ctagtgctta ggtcaaaatt ctactgtcac aacatctttt atataccact
    30241 gctttgcagt ttgtacatat ctgcatacaa accattttgc gctagaagtg tttcatgatt
    30301 cccgcgttct actatatgac ctttgtctaa aactaaaatc aattctgcat ctcgaatcgt
    30361 tgacagccga tgggcaatcg caatcgttgt ccgtccttga cgcatttttg ccaatccttc
    30421 ttggatcaat ccttctgttt cagtatcgat atttgctgtt gcttcatcca atactagaat
    30481 tttaggatca gtcacgattg ttcgcgcaaa agtgatcagc tgtcgttgtc cactagaata
    30541 gctggcacca tgttcgatca cttttgagtg atatcctttt ggtaattcat cgatgaattt
    30601 gtttgcttga acaaactcag ccgcatggcg tatctgttcg tctgtgatag ctggattcat
    30661 catccgaata tttcctgcga tatccccata aaacaagaaa gcatcttgca agaccagccc
    30721 cattttctta cgtaactctt ccattggata ttctcgaatg tcatgatcat caatcaatat
    30781 ctgaccttca taaaattcgt aaaaccgcat caatacgttg atgatcgagc tttttccgct
    30841 tccggtgtgt cccactaatg caacggtctc tcccggatta gcaacaaaag agatattgtt
    30901 taatacattg tgctctccat cataagaaaa gcttacattg cgaaactcaa tcttacctga
    30961 agtaatcatt tgatctgctc cagggttttg ttgtggtgca tattcttccg tatccataat
    31021 cttcaagata cgactaccag caaccatccc atctgtgaag atgctcaaaa agtccatcat
    31081 ttgagacatt ggattaaaaa atccctggac ataggtcgta aatgcataga tcagtccaac
    31141 ttctacggga gaatgcaacg catcgatccc aaacaaagtc aacaccaacg caatcgccaa
    31201 tgcatataga aaattgatga tcggacttaa cagaatcgaa ttcgttttga tcatcgcata
    31261 acgcgtgtct aaatactcat tgttcacttg ttcaaattct ttagctaaac gagcttcttg
    31321 gcgaaactgc tggataatac gcatgccgga aatcgattca ttcaatttcg tgttcagctg
    31381 acttaatttt tcccgcatat ttcggtaaat acgagaactg aatttctggt aataccagat
    31441 gacgattcct aaaatcggca aaaacaccaa agtccacaag gcaatttctc cattgatttg
    31501 aaacatggca aggaatgaag aaacgactgc aaagatccca gtcaatacca tcaaaaagac
    31561 gtaccaaaat tcaaatagcg tttctgtgtc attcgtcact cgagaaacgg ttgatcctgc
    31621 cggtgtctga tcaaaataac gcatacctag tgtatgcagc ttttcaaata attttaccct
    31681 aatatactga taggtcttca atgaggccat ggaatacaaa taccattgga aaaaccaaat
    31741 gatacttttt actatgactc caaataagta cataccagca aaaaacaaaa taacttgtgt
    31801 cgtagccgac tgtttagcca gatgatcatc cataaaagtt tggatgatcc ttggcaatag
    31861 gacattgatc acagacaaag caaacgcaaa taagatagca acgatgaacg ttcttctaaa
    31921 aggtttggcg aacttcatca gtcgtttgat gatctttttc tgttctttaa gtggaatcga
    31981 ttttgtccat tccgattgat aattttcatc catcaggcct cgcctccttc tatcttcgct
    32041 tctaattgct gtttctccca catttgttca taccagccgc ctagtgcaag cagctcatga
    32101 tgggttcctc gttcaatgat tttcccttga tcgacaacaa tgatttcttt tgcatgcatc
    32161 acgctactta aacggtgagc tgcaatgata gttgttttct cttttcttat ttcttttaaa
    32221 ttatgcaaga tagcttcttc tgttttggca tctactgcag agagtgcatc atctaatatt
    32281 aataattcag gatttttgat caatgctcga gcaatcgaaa tcctttgttt ctgtccgcca
    32341 gataaagaga ctccacgttc tccaactaag gtatcataac cttttggtaa ttccttgatt
    32401 tcattgttga taaatgctaa agaagccgca tgctctactt cttcttctga aagttttgga
    32461 ttagaaaaac ggatattatc tcgaatggtc atcgaaaaca agaagtgatc ctgaggcaca
    32521 tagccgattg atcctaataa agcattcaat gaatattctt tgatcgaacg tccgccaaat
    32581 tggattttcc cttgataatc atcgtattca cgcatcagta atttgatgat cgtcgttttt
    32641 cccgcaccag ttttcccaac aatacctaat gtttctcctt cttggatcgc aaacttgatt
    32701 tgttctaatg ctggtgattc ttctttcgga taattaaaag aggaaatagc tacattcagc
    32761 tcgccttttg ccggggtctg cactgcaccc agttgttcca cgatatgcgt cttttcatgc
    32821 aaaagttcat tgacgcgatc ataagaagca tttcctcttt ccaatacgtt gaacaatcga
    32881 ccaatcgcaa acatcggcca aacaagcata ccgatataac taataaatga gaccatctgc
    32941 ccgatcgaga tcgtgccttc caagacatac tttccaccta aaataattgt tgcaacataa
    33001 gacagaccga taatcaaggt aatgaatgga tcaaataagg agtctaaaaa attgactcgt
    33061 ttgtttttaa taattgcatc ttcaatcttt tcttcaaaat ccgctagatc ttctttttct
    33121 tgtccaaatg ttttaatgac tttgatccca gtgatacttt cttgtgtctt atcattgata
    33181 ctagaaaaag cttcttgcga atcacgaaaa gcatcatgga gttttgaccc cagcactcgt
    33241 gaagtgacgg ccagcaaagg cagaggaatc agcgcaatca gtgttaagcg ccaatcaata
    33301 aacaaaatca tcgcaatgat cgttgttccg cccgtaatca ctgaatctgc aaaggtcaag
    33361 ataccagctc ctgctacatt ctggatcgca ttcaaatcat tcgttgcgtg agccatcaaa
    33421 tccccagtcc gatatttttg gtaaaagaga ttgtccattt ctgtaaaatg tttgaataac
    33481 tgctgccgaa gttctttctc caatcgagct gcacttcccc aaatattcgt ccgccagata
    33541 taacgaaaaa gatattgtaa aaaagcagcg ataatcaata ggaagatcca cccgaaaatg
    33601 atctttattg ataaattttt atcggcgatc tcatcaatga tgatcccaat cacttttgga
    33661 ggaactaact gtaccaaggc cactgcgaat agcgagaaaa ccccaattaa ataatgtttc
    33721 ttttcctgac gaaaaaacca gcctaatttt ctaaaaatcg acattttact cctcctttgt
    33781 ctttatcaag agcctaggat tgtctgtgct tgtctttttt gctgaataat ctgaatagtc
    33841 taacaaatta cgaaataaaa aacggactga aaaaatcagc ccgtaaaaag tcgttgggtc
    33901 agaacctatt ttttcccttg gttcttcatt tgctgcatca tctggcgaat tttcttctca
    33961 gatggttttt gccccattga catcatcatt gatcgcaaca tgtcctcatt aacgggagga
    34021 tttttttcta aatagtcttt catatattta cgcgctagga aaaagccacc tactgcgccg
    34081 attagcaaag caataatagc aataagtacg acaatacctg ttgataacat ataaatctct
    34141 cctttctatt tccgaattgc tctcaatgac ttattttact aaagtttatt gaaaaaagaa
    34201 aggcgtattc tttagtttca tgcaaaaaat agtggttttt ttagaggaga acaaaatttc
    34261 tttcgtcatc tagcaagacg cgccatgaaa aaaaattagg cgtaccgaat ctttttcttt
    34321 aattttttct ttttgaggtg tagaatgtaa tcgtagataa ataggaaagg tggttttttc
    34381 ttgaataatc aatccaataa acaatttcat aagcatcgac ttattgaaca tacaaatggt
    34441 ttatctttag ctgaagtaaa tggaacagtt gatgtaccaa aggggaaaag tttttttaaa
    34501 acacttcttg cttactctgg acctggtgcg ttagtagcag ttgggtatat ggacccgggt
    34561 aactggtcta cttcgatcac cggaggacaa aatttccaat atttattgat gtcagttatt
    34621 ttgatgtcta gtctgattgc tatgctttta cagtatatgg cagcaaaatt aggaatcgtg
    34681 agccaaatgg atttggcaca ggcaatccga gcacgcacaa gtaagacatt aggtgtcatt
    34741 ttatggatct tgactgaatt agccattatg gcaacagata ttgcagaagt cattggtgca
    34801 gcaatcgccc tctacttact ttttgatatt ccattggtga ttgccgtctt tattactgta
    34861 ttagatgttt tcgttctctt actactgaca aaagtcgggt ttcgtaagat cgaagcactg
    34921 gttgtctgct taattttcgt cattttattc gtttttgtct atcaagttgg cttgtctaac
    34981 ccagattggc acagtttatt tgctggcttt atccctaaca gtcatacatt ctcagactca
    35041 ccggtcatcg gcggcgaaac tccgctgact ggagctttag ggattattgg tgcaacagtc
    35101 atgccgcata acttatatct tcattcagca gtttcacaaa ctcggaagat cgatcaccaa
    35161 aacgaagaag atgtagcaca agctgtacgt ttttctactt gggattctaa tatccagttg
    35221 tcaatggctt ttctagtcaa tgcactgcta ttgatcatgg gtgtcgctgt cttcaaaacc
    35281 ggttctgtcc aagatccttc cttttttggt ctgtatgaag ctttatctga tccatcagct
    35341 atgagcaatg gtattttgat tgctgtagca aaatctggta tcttgtccac tttatttgct
    35401 gtagcacttc ttgcttctgg ccagaactct actattactg gtacgttgac cggtcaagtc
    35461 atcatggaag gattcatcca tatgcgtatc ccgatttggc taagaagact ggtcacacga
    35521 ttgctttctg tgattcctgt attgatttgt gttttgttta caagtacaaa aggaacgatc
    35581 gaagaacatg ccgctttgaa taacttaatg aatgaatccc aggtgttctt ggcttttgcg
    35641 ttgccgtttt cgatgattcc tttactgatg atgaccaaca gccaagcgga gatgggtagc
    35701 cgatttaaaa attcattcat cgtaaaactt cttggctggt tctctgtgat tagtctaact
    35761 tacttgaact tacgaggatt accaggacag atcgaaagtt tcttcggcga gcaagccact
    35821 tccgctcaac tagcattggc tgatcacatc gcttatgtaa tcattgttct tgtccttctc
    35881 ctgttggcat ggacgattgg tgatttatat aaaggaaaca aaaaatatga acagcttttg
    35941 caggaaacgg tagaagaaat cgaataaaaa ctgatttttc cacacaaaga cccttgatag
    36001 ttaggttaaa aatctagcta ccaagggtct ttttatttag aagataaagc ttttttcaga
    36061 tttaatttac tgaaaactag catgcttgtt actacgatga attgcgttaa tggcacgcct
    36121 gcaccagtgt agacacttaa aaaatagtcc tttttttctg gcgcataaga acaaaaccat
    36181 gagacaccga aaagaaaaac caattgtgtc agtattaatc cggtgaccac aatcaaagag
    36241 gaactataat aggcacgatc agcttttgca aatacggttc ctagtatcat aagtaatatc
    36301 cctgctattg aagcaatcag cacgggaata gccagaactt ggtaaatttt tatgatgaca
    36361 ttcgcgactt ttgctgattt tgttaattgg aaattaggtg ttctcggttc attactatcg
    36421 atccaattct ccattagcgg aaggtttaat aattcttgag cctgtttgac ttgttcttgc
    36481 gttccgaaac tataatttcc tccttgatgg aaatctttat atcgccatgc ataagcgtat
    36541 cctgattgaa ggaaatcgaa tacgattggc agatcactca tcgtctttcc atctcctgat
    36601 cccatcagat agaactcttt tttctttttg attgttccct tgtgataggc ttcttctaat
    36661 tcttggttga cttttttcca aaaatcattg gtttcttttg cattgttttg gtaataacca
    36721 gattcgctta ccacatctcg caatgcccaa aagatgatat ctccgggtaa ttcttttact
    36781 tttccacctt gagtccacgc gtgagtcgta tataattcgt caatacgggt agaaacagta
    36841 tggaatgtag gagatgcggc taatgcttta tttagtgcag catgtgagac ccatatctca
    36901 ctgttttctt tattcaagtt cgtttcatca tctattcgga tcagttgttt catcaaacgt
    36961 ccaaattctc cactggaacg gtcattgatg gttgcaatac cgtatgttgt ctcattttta
    37021 tattcgatcc attgatagac gatggaaaag ccaactatag gaagtaaaaa caatgaaagt
    37081 ttcagccatt cccgtttcga gagaagaaag agtcgttttt ttaacttctt tacttctttg
    37141 aaagaaaagt cttgccctag caagatctgg acgaaactga tacccaaagc tactacagta
    37201 aaaggaagaa gccagataga atcttcgcga atgaaccaaa agaaaggaaa agcgacagaa
    37261 agccctaaaa accatggcag caattttctg ctagaaccgt atttgcggta ataaatagct
    37321 aataaacaag cgataatgat ctcaacagcc ggaatcacga gggcattacg atatgttcga
    37381 gtggaatatt catgtgtaaa actaatcgga gaaaacaaaa aataaaaata gatacttaaa
    37441 agaagatact tgttcttcac aatcggttta aacgcgagtg caaccaatat actggcaaaa
    37501 atactgaaca tgccgaacaa aactgcataa gaaagatgca gtttatgtgc aagtactaaa
    37561 aataagctgt aggagattcc tttggaaagt gttgtagtcg tatactcccc tagccattta
    37621 ccgtccaaaa tattttgagc atagcgaatc agcagctggt catcataggt tgccgacatg
    37681 tctgttccat agattgcttt actatataaa tacaaacgaa taattgtaaa taatagggct
    37741 atgccaagaa taaagtatgt atgtttcttt tttagttggt tgatcaaggg ttttaaagtg
    37801 ttcagcatct tttcgtcctt ccaatcacgt tttgccactt gcttgtgaaa cttttcgttt
    37861 ttttactttt ctaccagtat accttgtttg tctaaattta aaaagagggg ggagaagatg
    37921 catgatgaaa aagactgcaa aaagtactcc tttgtttctt aaacaaaaaa ggacaaggca
    37981 atgtgcctgg tccttttttg ctgctgtcag atagaaaatc ttagagtatc acttcttctg
    38041 ctaataactc aggaaacact tctggatcga atgtttgcgc tttgaacatg gcgatttctt
    38101 ttccatatgg tgcgtaagct ttctttttat ctttgcctac atagggagtt tccaagattt
    38161 tcgaaatatt gtcaaacatt ggatcatgga cgatcttatt cagcgtgtcg aatccgattg
    38221 ttccaaaacc aatattggcg tgccggtctt tatgcgaacc ttgcggattt ttagaatcat
    38281 tgatatggag tactttaagc cgatctaacc cgatgatctt atcaaactct tccattacac
    38341 ctgaaaagtc ttctttaata ttataaccgg catcattcgt atgacaggtg tcaaaagtaa
    38401 cggataattt ttcattcaat tttactccat cgatgatttg cgctaattcc tcaaatgttc
    38461 gaccgatttc cgtaccttta cctgccattg tctccaaagc gatttgtgcc gtctgttctt
    38521 ttgtcaaaac ttcattcaag ccttcaataa ttctagcaat tccggcttct gttcctgccc
    38581 ctacatgagc acctgggtga agtgtaattt gagtagcgcc taaagcttcc gcacgaacaa
    38641 tctcttctct cagaaattgg acggcaaaag gaaacatttc tggtttgatc gtattcccca
    38701 aattaatgat ataaggggca tgaacgacga tatttgttag acctttttct gccatgtacg
    38761 cttttccggc atcaatattc atctcttcga tggcttttct tctagtgttt tgaggggcgc
    38821 ctgtatagat cataaaagtc gaagatccat aactggctgc ttcttctgct gctcctaaca
    38881 gcattttctt tccgctcatg ctgacgtgtg agcctaataa catggatgga ttcctccttg
    38941 aaaggcaatt gttactaaca attcctatat attggtaaga tgatatacaa agaaaatgat
    39001 tgatttttct ttgccctgtt ctccatagta actgaaattt tctctaaaga aaagcttaag
    39061 aactctttta taataaagat cattttctca cgttttaact cttttaaaaa agaaattcct
    39121 gccaaaaaca gccttgtctg tttttttgtt ctattatttg ctataattag cgaaataaaa
    39181 ccattagttg aggtgaccct tttgccacgc aaaaatactc gaaaaaaaca gaatagaaga
    39241 aaaaaagaaa aatggtttgt tcctaagatt atttttagag tttttcaatc attgactgtt
    39301 tttattactg ttcttctttt gatgttcgca gctctaggta ttggaatagg cgctggctat
    39361 tttgcctatc tcgtcgaaga cacacaattg ccgactaaaa aagcactgca gacagaacta
    39421 ggaaacatta cagaaacgtc aaagattgtt tatgcagata atacagagat atcaaaaatc
    39481 cagacagatc tgatgcgcac gactatttca tctgataaga tttcaccttt attgaaaact
    39541 gcaattatca gcacagagga tgaatacttc gataaacacc aaggttacgt tcctaaagct
    39601 gttttacgag cattagtatc tgaagctact ggtattggct cttctggtgg atcaactttg
    39661 acacagcaat tagtcaaaca gcagatcctt acagatgaaa caacattcaa acgtaaagca
    39721 aacgagatca ttctttctgc acaagtagaa aaatactttt caaaagatga gatcattgct
    39781 acgtatttga atgtctcacc atttggtcgt aataataagg gacaaaacat tgctggagtc
    39841 caggaagctg caaaaggaat ctttggtgta gatgcaaaag atgtgacgct gccgcaagct
    39901 gcttatatcg ctggattacc ccaaagcccg atcacgtata gtccatacac gaacacaggc
    39961 gcgctaaaag aagatctttc tgccggcctt gccagaaaag attttgtttt attcagtatg
    40021 tatcgtgaag gccaaattac gaaagaacaa tacgaagaag caaaagccta tgaccttaca
    40081 aaagactttc taccacaaca aatcgcagaa cagactgaca gagaattctt gtattatact
    40141 gtgatgaatg aagcaacaag aatcattgca caacaattgg ctgaaaaaga caacgctgat
    40201 atgtctgatt catcggtcag tgacgcttat tatcaaaagg cacagcaaac gatccaaaat
    40261 aaaggatata caatccattc gacaatcgat aaagatgtct atgctgcatt gcaaaatggc
    40321 gttgaaaatt atggatacat gttagatgat ggtgctggca gtcaagtaga aaccggaaat
    40381 gtattgatgg ataacagaac tggccgcatc tacggattcg ttggcggacg gaattacagt
    40441 cagaatcaaa ataaccatgc cttcgataca gaacgtcaag ctggctcatc aatcaaacca
    40501 gttcttgttt atggacctgc aatcgacatg gggcttatcg gatcagaatc ccgtgtatca
    40561 gattatgcaa caacttggca agaaggagaa aatgctggcg agaaaatcgt caatgctact
    40621 aacgaaggat cgaacacctt ccaaaccgtc cgtgagtcac tagaatggtc caacaatatc
    40681 ccggcttatc acttatatca agatgtgttg aattccggtg gatcgaaaca atatgcttac
    40741 gagcactact tggcaaagat gaactacccg gcaaatgaca attggggcgt tgaatctgct
    40801 cctttaggta cagtagatgt cacgacgctt caacagacca acggatttca ggctttagca
    40861 aacggcggcg tctatgaaga aggctatatc atcgattcca tcacagataa cgcaggaaat
    40921 gtaatttata aacacgaaag caactctgtc cggatctatt cggaagcaac ggcatcgatc
    40981 atgaatgaca tgatgcgtag tgtgatcaat gcaaaaatca ctacaccatt caaagatgcc
    41041 atctcaagct taaacggaaa tctaggaaaa gcagattggg tcggaaaaac tggttctacc
    41101 aatgaatacc gagattcatg gctaattgtc tctactccgt ctattaccat cagtagctgg
    41161 gcagggcacg atgacaatac tggaatggac agcaaagcaa ggatacgttc ggctaattat
    41221 cttgccaatc tgatcaatca agcttaccaa gcaaaacctg acatttttgg cactgatgag
    41281 aaattcgaat tatcatctga tgtgatgaag aaaaaagtat ctggatttac tggtcaaaca
    41341 cctggaaaag ttactgtcaa taaaaaatcc atccaaacac caggaaaaac agttgaatcg
    41401 ctatgggcga aaaatggacc gaagaaaagc gcctttaaat ttggcgttgg cggtaccgat
    41461 gaaaactata cagattactg gaacaccgct tcagcttatg caagagcgaa tcaagcaact
    41521 aaagaatcag atgagaaaga tgattgattt aatagaatac acagtaataa gggattgtga
    41581 caatagtatt gtcacagtcc cttatttttt tgatggtggt gaacaaaatg cagcatctat
    41641 acacggattg ttttatatac gataaatgta atttcaacca ctttctctct tttttaagac
    41701 acaaaaaaag gattataaca aaattttgtc ataacccctt ttcccaaata aacggtgttc
    41761 tcctgccaac tccgaatcac gagtcacaat attcatcaaa catgagatgc tgttttttga
    41821 aattgctcct tgtaacgcag agttaaacgg caggcacaca acctctgaat ggttgtcctt
    41881 ttattatttt gtagatcctt tttcttcgat tccataaggc aagataatcg ttttttcttc
    41941 gacttcttct ttattcatca ttttcgtcaa taggcgcata gataccgccc ctaaatcgta
    42001 taatggttgt gtgatacttg ttaaacgagg acgagctact tctgtcagta gtgaattgtt
    42061 acttgtgatg atttcgaatt cttccggtac tttcacgcca cggtctaata aaccatccaa
    42121 aataccgatg gctaattcat catctgaaac atagacagct gttgcaccgc tattatggat
    42181 ccgatcgact agagatagac catctttgaa gttgtattgt gcttcaaaga tcaatccttc
    42241 gctatatggt aggttatttt cgcttaatgc ttctttatag cctttcagac gattttgtcc
    42301 attgatggga tcgatcaacg caccactgac aaaagcgatt tttttattgc cgctttttgc
    42361 caatttgttt actgcatctt ttgttgcgga tacataatca atgtttacgc tacctacttg
    42421 ttcatctgga tcaatagagc cagctaatac aacaggagtt tttgaacgtg aaaattctcc
    42481 acggatttcg tctgtgatat ggtgacccat gaagatcacg ccgtccactt gcttagccag
    42541 taagttattc aatacattga cttctttttg gtcgttgcca tctgaatttg ctaagataat
    42601 attgtatttg tacattgttg ccacatcatc gataccacgt gctaaagaag caaagaacat
    42661 attgctgaca tcagggatga ttacaccaac tgttgttgtt ttcttacttg ccaagccacg
    42721 cgcaactgcg tttggacgat aatccaagcg atcgataact tccaagactt tttttcttgt
    42781 tgctggttta acgtttggat tgccatttac gacacgtgaa acagtagcca tcgaaacatt
    42841 tgcctctctc gctacatcat agatagtgat tgtttgcttt tccatttgtg ttctcctatt
    42901 catattttat atgttttctg aaaatacgtc ttacatttct aaaatagaat atcaaataat
    42961 ctcaagaatt gcaaccgttt tatctctgtt ttcatgaaaa tttttacttt tttcagtttt
    43021 tagaacgttg gtcttttaat aaatcagtgg tacaattatt gaatacaaaa ttaatttaga
    43081 aagtaggttg ccaaatgaat aacgtaaaaa tcaatgaact acgtcaatgg atggcacaac
    43141 accaagtcga attggcttat atcagcgacc ctggccacat cgcttacttt tccggatacg
    43201 aaagtgatcc tcatgagcgt gtactagctc tttttatccc tcttgaacaa gatcctttcc
    43261 ttttcactcc tgctttggaa gttgaagatg ctcaaaacag tagttgggcg tatgatgtcc
    43321 gtggctattt agacagcgaa aacccttggg agatcatcgg aaaagaactt cgtgctcgct
    43381 atggtcagcc tggcaatgta gggattgaaa agaacgcctt gactgtcgag cgcttcgatg
    43441 ctatctctga tatcttgacg ggaacggatt cttacactga tctaacttcc atcatccaac
    43501 gtctacaact gatcaaaact ctagaggaaa aagacaaact gatcgaagct ggtaaatggg
    43561 ccgatgttgc atttgagatt ggctttaaag cagtgaaaga aggcgtgact gaacaagcga
    43621 tcattgcaga aattgaatac caattgaaaa aacaaggtgt ttcacaaatg tctttcgaca
    43681 cattagtcct tgcaggagca aatgctgcaa gccctcatgg tacaccagga aatacgaaga
    43741 tttcaccgaa tgaacttgtg ttattcgatc ttggcgttgt ttggaatggc tattgcagtg
    43801 atgctactcg tacaattgct tatcaaaaac caaccgactt ccaagaaaaa atctacaaca
    43861 tcactcttga agcacagctt gccgcacaag aagccgtccg tccaggtgtg acagcaggtg
    43921 aacttgatca aatcgcacga aacgtgatca acagctatgg ctacggcgaa tatttcaatc
    43981 accgtttagg tcacgggatc ggaacaaccg tccacgaatt cccttccctt gtagaaggaa
    44041 atgacttagt aatcgaagaa gggatgtgct tctctcttga accaggaatc tatattcctg
    44101 aaaaagtagg ggtacgtatc gaagactgtg tatatgtgac aagcgatggc tgtgtgccat
    44161 tcacgactac accaaaagaa ttacttgttt tagaaaaata agagtaaaaa aggtgagagc
    44221 tagtccgctc ccaccttttt attctataaa tttgtgattt tagtgatttg tagatgaatc
    44281 tgtcattgta tcttttattt cttttttagc ttctttcgca tcttcttttg ccattttcat
    44341 catttctttt gagtcatctg caactggttt tgcttctgac attccttcat tgacagtatc
    44401 tcctgcttta ccagcagcgt cttttacatc aatcatgata tcttctgaag tatctttagc
    44461 aatacttcct aattcatctg caggtttttt catatcttct gccgtttttt tgaaacgatt
    44521 agataagtca gctgattgtt ctttaagatt taacaagata tcgtcagatt cacttcctgc
    44581 ttcatctgct gattcactta ctttttgttt gacactacct gctagatcac ctgcttgttg
    44641 agaaagatct tgcgcttttt cttttgcaat acctgttaac tcagtcgatt tttctttcgc
    44701 catatctgtg taatctgaag ccatatccat caaatcatcc gcttgggcag ctaattcatc
    44761 ccgaagttct ttaccagatt ttggtgctaa caataacgcg gcgattgctg cggctgtgcc
    44821 tccgactact gcacctaata aaaatccacc ttttttagcc atttttattt tcctcctgtc
    44881 gtttcagtat gttttttgtt tttaaaaaat ttcatcgctg tcccaccgac ttttccggca
    44941 accgtcgctt tagctgtcgt tttaccgatt ccgccgactt tcgtcgcgat gtttttacta
    45001 gaatgattaa gttctgatac actttcactt aaatcagcaa ctgctgtgaa caatggatcg
    45061 atcgttgcga cttttccatt gatatccaag agcagttcat tacttttgac cagtaagcct
    45121 tctacttgtc tagacaaaag atctacgtct tttgtgacga tttcgatcgt cgtatttgct
    45181 tctgctacag tctgatctac tttttctaaa gttttagaaa cttgcaacaa cacacgaacg
    45241 ataaaaacga caagtatcgc aaaggcgatt gctgcgatca atcctgcaat ttctcctcct
    45301 gacatgatca aactcccttc ttgtctctct tttctataag gatagcattt tcgtgttttt
    45361 taaacaaata aaaacgagct tcaactactt tttattcaat tagcagctga agctcgttag
    45421 aaaaaagggt tattttttct tttcttcttt gatttctgtt attccttttg gattatgatc
    45481 gatcttgctt ccttctgctt ctaatgtttt acctaaagac aggatgtact cacgtaaagg
    45541 tgcacctact tctggatgtg ttagaccata ttcaatactt gttttcataa aaccaaattt
    45601 gtcaccgaca tcgtaacgtt tacctgtgaa tttacgggca aatacgcgtt gtgttttatt
    45661 caatgtatcg attgcgtcag ttagttggat ttcatttccg gctcctggtt gttgctcgga
    45721 caatacatca aaaatttctg gagtcaataa gtaacgtcca ataatagcta aatcacttgg
    45781 tgctttgctt ggatctggtt tttcaacaaa gtttttgaca ttgtataacc catcttccaa
    45841 tacttctcct ggattgataa ttccgtattt tgaagtttca tcatgaggaa cttgcataac
    45901 ggcgatcgta gaagcatgag tacgttcgta atcatcgatt aattgctgtg tcaatggtac
    45961 ttcgtcttcc attatatcat cgcctagcat aacgacaaat ggttcatttc caataaaggc
    46021 tcttgcttga agaacggcat gtcctaaacc ctttggatga gattgacgga tgaaatgcaa
    46081 gttgacatct gtcgtttctt ctactaattt caataattcg gtctttcctt tttcacttag
    46141 gtttgtttct aattcaatat ttgaatcaaa gtgatcttcg attggtcgtt tagcttttcc
    46201 tgtaacgatc aagatgtctt cgatacctga tttcaacgct tcttcaacga tgaactggat
    46261 tgttggttta tcaacaattg gcaacatttc ctttgccata gcttttgttg ctggtaaaaa
    46321 tcttgttcct aatcctgctg ctggaataac tgctttttta actttcatat acattcgctc
    46381 cttcaaaaaa ttgttaatga gaattcattt tctttttttc catcacgtaa catgatttct
    46441 tttgcggctg ttttgatttc tttttgttca tacagcactt catagattgt ttgagtgatg
    46501 ggcatttcga tttccatcat ttgggctagt tccatcgctg cttgggtagt attcacacct
    46561 tcaacgacca ttcccatatt ggctagcacc tcttctaggg gctgcccttg tcctaacaag
    46621 ttgcctgcgc gccagttcct agaatgaaca cttgtgcaag taacgactag atcgcctatt
    46681 ccgcttaatc cgataaaggt cagcgggttg gcacccattg ctacacctaa acggctgatc
    46741 tcagctaatc cgcgcgtcat gattgctgcc tttgcattat cgccaaaccc taagccgtga
    46801 atagcgccag caccaatagc aataatattt ttcaaagctg cccccatctc tacaccgatc
    46861 acatcaggat tggtatagat ccgaaaataa ttattcatga acagctcttg tacatatttt
    46921 gctgcttttg catctgtact tgcagctgtg atcgtagtga tgtcatgttt tgctacttct
    46981 tctgcatggc ttggtccaga aagaacaaca acagcttttc ttttttctgc tgggatttcc
    47041 tctaacaaga tgtcagagat ccttttatgt gttccctgtt ctaatccttt acttgcatgg
    47101 atgatcactg gctttgtatt caatttaggc actagttctt ttgcaacaga acgtattgct
    47161 ttcgttggaa tgacgaatag caccgcatct gcgtctttta ctgcatcttc catattggtg
    47221 aatgcttgga tggaatcggg tagataatga tctggtaaat aacgttgatt cgtgtgttgg
    47281 gtgttgattt cttctacttg tgaagcacga tgtccccaaa tccgaacgtc atgtccgttt
    47341 tcggcgagga cttgtgccaa agcagtaccc caagagcctg gtcctaaaac cgctactcta
    47401 taattcattt taccactcct gaaatttttt tcatttttaa cgctctaaaa atcagataat
    47461 ttgttgattt tttcctaatg agcggtcata atccggtaaa tttccttttt ttctgcgcca
    47521 aataaataga atcaatgtac cgacaaataa cacagcagat aataattgag aaatacggat
    47581 cacacctaaa aagtaaagac tgtctgtacg taatccttca ataaagaaac gcccgaaact
    47641 ataccacatt agataacata aaaggacttc gccttttttc aaaaggtgtg gtttctttcg
    47701 taaaaacaaa agcaaaataa atccaatcaa actccaaagt gattcataaa gaaatgttgg
    47761 ttgacgatag atcccttcga tatacatatt atcaatgatg aattgcggta aatgcagact
    47821 ttctaagaag cttcgcgtcg tgtctggtcc atatgcttcg tggttcatga aatttcccca
    47881 acgaccgatt gcctgtgcta ataatacact tggtgcagcg atatctaaaa aagtccacaa
    47941 agaaataaaa tgatatttag taaaaaaata cagcgtgatc gcgcctgcaa tcagtccgcc
    48001 ataaatagct aagcctcctg aacggatatt gaatatttgg attggatcag ctatatactg
    48061 gggaagatca aataaaacat aatataagcg agcgccaata atggagatag gcaatcccca
    48121 caacataaaa tctgtcacat catctgcttt taatcctacc cgaacagcct ctctgctgct
    48181 aagccacata gcgataatga tacctgatac gatgatcaat gcataccagt agataggaat
    48241 ccctacaagc tcaaaagcaa ctcgattgat ttgtgctaac atattgtcac tccttgctag
    48301 tcgttgcctg agttttcttc aatcaagcga cttaatcttt cttcaaatgt tttcgttgcg
    48361 tcaaagccca ttgtacgtgc acggaaattc atcgcggcta cttcaatgat gattgcaacg
    48421 tttcgtcctg ttttgacagg gatttttatt tgtggaatgc ccacttccgc gatttcaacg
    48481 gtcgtgtctt cagagcccag acggtcgtat ttcttatctt ttgcccatgc ttccagataa
    48541 acagccaact ggacttgcat agagccacgt accgcactcg caccaaaaag attcattaca
    48601 tcaatgatcc cgattcctcg gatttcaatc agatgttcca ggattttagg cggttctccg
    48661 atcaccgtta attcatcttg ctgataaaca tctactcgat cgtctgcgat caatcgatgg
    48721 ccgcgtttga tcaactccaa cgctgtttca ctttttccta tcccactgtc accttgaatc
    48781 agtacaccta atccgtaaac atccactaaa acaccgtgga cacttgttcg aggggcaagg
    48841 cgaccatcta aataactgga taattctcct aataaacgag aagttgagat gggagaacgc
    48901 agtacagcta tcccattttc atttgctgcc tggatcattt cttctggagc tggtaatcct
    48961 cttgaaataa taaatgcggg catatcttca ctacataatc ttctcatgat gatcaaacgc
    49021 tcttctggga tcatccgttc cgcaaaactg atttctttac tgccaaatag ctgtaaacga
    49081 ttatgggaat aataattaaa ataaccagtc aactctagtc ctgggcgtga gatgtcccca
    49141 gttacgatga cacggtctaa gcttttctcg tcgcctgtca cgatttccaa agataatttt
    49201 ttcactaatt ggctaacttt tacaacttct gacattcatg ctacctcatt tctattagag
    49261 tgttctctat cattttatca ttgtctagcc aaaagtacta ccaattaaaa
//
LOCUS       DACDLI010000013        48023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDLI010000013 DACDLI010000000
VERSION     DACDLI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.013"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..439
                     /locus_tag="IV353_000788"
     CDS             <1..439
                     /locus_tag="IV353_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9009059.1"
                     /translation="TDCKICFKYGLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEP
                     YLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFF
                     TNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            537..725
                     /locus_tag="IV353_000789"
     CDS             537..725
                     /locus_tag="IV353_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9009060.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(783..1136)
                     /locus_tag="IV353_000790"
     CDS             complement(783..1136)
                     /locus_tag="IV353_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9009061.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1667..2089
                     /locus_tag="IV353_000791"
     CDS             1667..2089
                     /locus_tag="IV353_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9009062.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2086..2316
                     /locus_tag="IV353_000792"
     CDS             2086..2316
                     /locus_tag="IV353_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9009063.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2814..3014
                     /locus_tag="IV353_000793"
     CDS             2814..3014
                     /locus_tag="IV353_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9009064.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3041..4234
                     /locus_tag="IV353_000794"
     CDS             3041..4234
                     /locus_tag="IV353_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9009065.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4302..5867)
                     /gene="guaA"
                     /locus_tag="IV353_000795"
     CDS             complement(4302..5867)
                     /gene="guaA"
                     /locus_tag="IV353_000795"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9009066.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5926..6849)
                     /locus_tag="IV353_000796"
     CDS             complement(5926..6849)
                     /locus_tag="IV353_000796"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9009067.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7045..7650
                     /locus_tag="IV353_000797"
     CDS             7045..7650
                     /locus_tag="IV353_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9009068.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7958..8974
                     /locus_tag="IV353_000798"
     CDS             7958..8974
                     /locus_tag="IV353_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9009069.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9115..10416
                     /locus_tag="IV353_000799"
     CDS             9115..10416
                     /locus_tag="IV353_000799"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9009070.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10462..11124
                     /gene="deoC"
                     /locus_tag="IV353_000800"
     CDS             10462..11124
                     /gene="deoC"
                     /locus_tag="IV353_000800"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9009071.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11145..11543
                     /locus_tag="IV353_000801"
     CDS             11145..11543
                     /locus_tag="IV353_000801"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9009072.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11609..12691
                     /locus_tag="IV353_000802"
     CDS             11609..12691
                     /locus_tag="IV353_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9009073.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12903..14465
                     /locus_tag="IV353_000803"
     CDS             12903..14465
                     /locus_tag="IV353_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009074.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14458..15591
                     /locus_tag="IV353_000804"
     CDS             14458..15591
                     /locus_tag="IV353_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009075.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15591..16547
                     /locus_tag="IV353_000805"
     CDS             15591..16547
                     /locus_tag="IV353_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009076.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16646..17812
                     /gene="deoB"
                     /locus_tag="IV353_000806"
     CDS             16646..17812
                     /gene="deoB"
                     /locus_tag="IV353_000806"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9009077.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17836..18654
                     /locus_tag="IV353_000807"
     CDS             17836..18654
                     /locus_tag="IV353_000807"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9009078.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18671..19378
                     /gene="deoD"
                     /locus_tag="IV353_000808"
     CDS             18671..19378
                     /gene="deoD"
                     /locus_tag="IV353_000808"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9009079.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19676..20362)
                     /gene="gpmA"
                     /locus_tag="IV353_000809"
     CDS             complement(19676..20362)
                     /gene="gpmA"
                     /locus_tag="IV353_000809"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9009080.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20534..21115)
                     /locus_tag="IV353_000810"
     CDS             complement(20534..21115)
                     /locus_tag="IV353_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9009081.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21248..21931)
                     /gene="rpiA"
                     /locus_tag="IV353_000811"
     CDS             complement(21248..21931)
                     /gene="rpiA"
                     /locus_tag="IV353_000811"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9009082.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22250..22663
                     /gene="rpsL"
                     /locus_tag="IV353_000812"
     CDS             22250..22663
                     /gene="rpsL"
                     /locus_tag="IV353_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9009083.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22747..23217
                     /gene="rpsG"
                     /locus_tag="IV353_000813"
     CDS             22747..23217
                     /gene="rpsG"
                     /locus_tag="IV353_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9009084.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23311..25398
                     /gene="fusA"
                     /locus_tag="IV353_000814"
     CDS             23311..25398
                     /gene="fusA"
                     /locus_tag="IV353_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9009085.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25570..26757
                     /gene="tuf"
                     /locus_tag="IV353_000815"
     CDS             25570..26757
                     /gene="tuf"
                     /locus_tag="IV353_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9009086.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27164..27472
                     /gene="rpsJ"
                     /locus_tag="IV353_000816"
     CDS             27164..27472
                     /gene="rpsJ"
                     /locus_tag="IV353_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9009087.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27503..28132
                     /gene="rplC"
                     /locus_tag="IV353_000817"
     CDS             27503..28132
                     /gene="rplC"
                     /locus_tag="IV353_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9009088.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28160..28783
                     /gene="rplD"
                     /locus_tag="IV353_000818"
     CDS             28160..28783
                     /gene="rplD"
                     /locus_tag="IV353_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9009089.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28783..29073
                     /gene="rplW"
                     /locus_tag="IV353_000819"
     CDS             28783..29073
                     /gene="rplW"
                     /locus_tag="IV353_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9009090.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29112..29945
                     /gene="rplB"
                     /locus_tag="IV353_000820"
     CDS             29112..29945
                     /gene="rplB"
                     /locus_tag="IV353_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9009091.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29988..30266
                     /gene="rpsS"
                     /locus_tag="IV353_000821"
     CDS             29988..30266
                     /gene="rpsS"
                     /locus_tag="IV353_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9009092.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30289..30636
                     /gene="rplV"
                     /locus_tag="IV353_000822"
     CDS             30289..30636
                     /gene="rplV"
                     /locus_tag="IV353_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9009093.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30650..31306
                     /gene="rpsC"
                     /locus_tag="IV353_000823"
     CDS             30650..31306
                     /gene="rpsC"
                     /locus_tag="IV353_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9009094.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31309..31743
                     /gene="rplP"
                     /locus_tag="IV353_000824"
     CDS             31309..31743
                     /gene="rplP"
                     /locus_tag="IV353_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9009095.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31733..31921
                     /gene="rpmC"
                     /locus_tag="IV353_000825"
     CDS             31733..31921
                     /gene="rpmC"
                     /locus_tag="IV353_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9009096.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31946..32212
                     /gene="rpsQ"
                     /locus_tag="IV353_000826"
     CDS             31946..32212
                     /gene="rpsQ"
                     /locus_tag="IV353_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9009097.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32270..32638
                     /gene="rplN"
                     /locus_tag="IV353_000827"
     CDS             32270..32638
                     /gene="rplN"
                     /locus_tag="IV353_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9009098.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32675..32983
                     /gene="rplX"
                     /locus_tag="IV353_000828"
     CDS             32675..32983
                     /gene="rplX"
                     /locus_tag="IV353_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9009099.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33011..33550
                     /gene="rplE"
                     /locus_tag="IV353_000829"
     CDS             33011..33550
                     /gene="rplE"
                     /locus_tag="IV353_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9009100.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33569..33754
                     /locus_tag="IV353_000830"
     CDS             33569..33754
                     /locus_tag="IV353_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9009101.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33790..34188
                     /gene="rpsH"
                     /locus_tag="IV353_000831"
     CDS             33790..34188
                     /gene="rpsH"
                     /locus_tag="IV353_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9009102.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34220..34756
                     /gene="rplF"
                     /locus_tag="IV353_000832"
     CDS             34220..34756
                     /gene="rplF"
                     /locus_tag="IV353_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9009103.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34917..35273
                     /gene="rplR"
                     /locus_tag="IV353_000833"
     CDS             34917..35273
                     /gene="rplR"
                     /locus_tag="IV353_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9009104.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35294..35794
                     /gene="rpsE"
                     /locus_tag="IV353_000834"
     CDS             35294..35794
                     /gene="rpsE"
                     /locus_tag="IV353_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9009105.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35809..35988
                     /gene="rpmD"
                     /locus_tag="IV353_000835"
     CDS             35809..35988
                     /gene="rpmD"
                     /locus_tag="IV353_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9009106.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36032..36472
                     /gene="rplO"
                     /locus_tag="IV353_000836"
     CDS             36032..36472
                     /gene="rplO"
                     /locus_tag="IV353_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9009107.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36472..37767
                     /gene="secY"
                     /locus_tag="IV353_000837"
     CDS             36472..37767
                     /gene="secY"
                     /locus_tag="IV353_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9009108.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37829..38476
                     /locus_tag="IV353_000838"
     CDS             37829..38476
                     /locus_tag="IV353_000838"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9009109.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38667..38885
                     /gene="infA"
                     /locus_tag="IV353_000839"
     CDS             38667..38885
                     /gene="infA"
                     /locus_tag="IV353_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9009110.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38918..39034
                     /gene="rpmJ"
                     /locus_tag="IV353_000840"
     CDS             38918..39034
                     /gene="rpmJ"
                     /locus_tag="IV353_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9009111.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39052..39417
                     /gene="rpsM"
                     /locus_tag="IV353_000841"
     CDS             39052..39417
                     /gene="rpsM"
                     /locus_tag="IV353_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9009112.1"
                     /translation="MARIAGVDIPRDKHVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39445..39834
                     /gene="rpsK"
                     /locus_tag="IV353_000842"
     CDS             39445..39834
                     /gene="rpsK"
                     /locus_tag="IV353_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9009113.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39914..40852
                     /locus_tag="IV353_000843"
     CDS             39914..40852
                     /locus_tag="IV353_000843"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9009114.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40880..41260
                     /gene="rplQ"
                     /locus_tag="IV353_000844"
     CDS             40880..41260
                     /gene="rplQ"
                     /locus_tag="IV353_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9009115.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41532..42371
                     /locus_tag="IV353_000845"
     CDS             41532..42371
                     /locus_tag="IV353_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9009116.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42347..43216
                     /locus_tag="IV353_000846"
     CDS             42347..43216
                     /locus_tag="IV353_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9009117.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43206..44003
                     /locus_tag="IV353_000847"
     CDS             43206..44003
                     /locus_tag="IV353_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9009118.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44206..44952
                     /gene="truA"
                     /locus_tag="IV353_000848"
     CDS             44206..44952
                     /gene="truA"
                     /locus_tag="IV353_000848"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9009119.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45285..47954)
                     /locus_tag="IV353_000849"
     CDS             complement(45285..47954)
                     /locus_tag="IV353_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9009120.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        15484 a         8821 c        10255 g        13463 t
ORIGIN      
        1 gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta gtactccagc
       61 agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag ctggaacaga
      121 attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc tttcacgggc
      181 atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga aaaataatga
      241 ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca atgatttaac
      301 tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc aggttaccac
      361 tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag taagatatat
      421 gttcaataaa ataacttagt gcgttttatg ttgttatata aatatggttt cttattaaat
      481 aagatgaaat attctttaat atagatttga attaaagtgg aaaggaggag attgttatta
      541 taaactacaa gtggatattg tgtcctattt gtggaaataa aacaagacta cgaatacgag
      601 tggatactat acttaaaaat ttccctttat actgccccaa atgtaagaac gaaactttaa
      661 ttaatgttca aaaaatgaat ataataacaa tcaaagagcc agacgccaag acgcagagcc
      721 gataatttga gaaatgaaac tctcatctta tcggctcttt ttgtttatct gaattttact
      781 gactagcctt caatatttct tgcttcattg ataccttttg ctagtgcttc cattaaggat
      841 agttctttgt ctgtaaagct atccatgtat ttttctacct gtaaccttcg agtgctttta
      901 atcaaattat ctgtaggtaa gaaaaattca tcaatagata catggagtaa tgatacaaga
      961 tcatacagca cttgtgtact tgggtgttgt cctttgtttt ctatatttgt taaataacgt
     1021 gggtcaattt caatcattgt tcccacttgt tcacgagtga gaccccgttt cattcgagcc
     1081 tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat cttctttttt acgcatacta
     1141 aatcacctct atacatttta ctgttcctat ataaccataa acaggtatag aaaaacatgt
     1201 tatatagttt atgagttcct atagaacact gtggaattta ttaatgcgat gaatccaatt
     1261 tcaaatagaa aggtcgctga ttttatttac ctatacaact ttataaaatt tagtcatact
     1321 cctagaaagt agataaaatt ggcgacttaa tggagggaaa agtcgccatg agaaagataa
     1381 tctgtcatct agttctctta attaagttct tatcaaaaaa acttatccac ctgtactgga
     1441 tagatatgac catcaatgtg gttttgcaga ttacatagat agaaaaatca aagcatatga
     1501 acagagaaaa caatctgttt atgtgctttt ttggtcactt aataaatatc ttttaggatt
     1561 tctcttcatt tatgcaacaa atcccccgtt cgtgtcgggg tttagtatga agggagataa
     1621 agaagcacat gccactccct ttcacgacag aaagggggtg aaaagcatga aaccatcttc
     1681 ttttcaagaa accatagaaa atcagtttga ctatatctgc aaacaagtta ttgaagatga
     1741 acgaaaagat tacttcaaac atctagacag actatcagca aaagaagtct cgttttctga
     1801 tatagataac taccttgtca actctttttc tactgttgat acgtacgtca ctgatttcca
     1861 actatttaaa ttatttggat taagaatcaa tgttgaaagt gatttattaa gtgaagccct
     1921 gcgtcatctt tcagagaaaa aacggaacat tattttactt cattacttta tggatatgag
     1981 tgatgtggag attgcggagt tactaaattt gaatcgttcg actgtttatc gtcatcgaat
     2041 aagtggacta gccatgatta aagaattcat gaaggagtgt aaagaatgaa aacagcttat
     2101 ccaagggttc ccttccccct cattgtaaag gcgacagatg gcgacgtgga agcaattaat
     2161 cagattgtta aacattatcg agggtatacc tcaaaacgtt cattacgtcg catgacagac
     2221 gaatatggaa attctcatat ggttattgat gaaaccctac gtggcagaat ggaaacgaaa
     2281 ctcatctcta agattttatc gttcgaaatt aaatagtccc attttttcgt ggaagcgtgt
     2341 cgaatcgtat ccacgcttcc cgatttggga ggtttgttat tccatcaaag catattgtgt
     2401 gttcagtagg ttttgatagg ctaacaagcc aaacgttctt tgacaaccaa ataaaggcaa
     2461 tcgaatacgt ttcggtaaga aaagagctga tgaactaaca cgccatgacc tacaaaatta
     2521 gtgagcgatt catgttagta attcgaaagt aatctttagc aggattgctg ccatgacttt
     2581 cttatcgtga taatgatact cccgtatagc catagtccga gcgttagaag cgtcacaggc
     2641 aatgggtacg gctacatgag aaccatgcag aagtgaaatt cctgtgagct ttgctagagc
     2701 tgttcgattg ctaagatatt cttctcttgt cagtgtatta ttataaaact atttgatata
     2761 attataggtg aatattggag ttttcctagc ctataattgg gaaaggagta ataatgataa
     2821 cggtcacaaa aaaggattta gttgagctag gatatggaac ttctttttca gcagacatca
     2881 taaaaaaagc aaaagaattg atgattacaa aagggcatat ctactatcaa tctcgaaagc
     2941 tagatagagt ccctcgtgaa gctgttgaag aaattctagg aattcggttt tcaaatgaaa
     3001 acaggtctga gtagtttttg cacagtaagg agtaaaaact atggttaaag acccaataaa
     3061 aaaagcaaag aatggaacct attattttcg tgcaaatcta ggttatgatt ctaaagggaa
     3121 aaaaattcaa aaatatcgta gtggattcac tactaaaaaa gaagcaagag aagaatattc
     3181 gaaactgcta ttgatgaaac cagaagaatt aagtgagaat aaggataaaa tgacgtttaa
     3241 acattatatc tttgaaattt tccttccatg gtataagaca caggtaaagc tcagaacata
     3301 tgaaaatcgc ttaccaacta ttaagaagca tttctcttat tttgataaaa tggctgtttc
     3361 cgatattgag cctattgatg tgcaaaattg gcaactaaaa ttatcaaaga agtgtaagtc
     3421 ttcttatgta agagctgttc aaggactatt ttcagttgct atggatcgag caattgtcct
     3481 tgggataacc accacaaatc catctaagat tattggcaat gtgaaaaagc aaaagtctaa
     3541 aattgaattt tggacaaaag aagaatttga aaaagtaatc tctctaattt acaaagaaga
     3601 ttactatcaa cactttttat ttatctctct gtggttttta tttatgactg gaatgcgaat
     3661 tggtgaagct actgccattc aatgggaaga tattgatttt gactcaggag tactaaccat
     3721 caacaaaaca ctctattaca agaatcaatc gaattataga tttgttgaac caaaaacaaa
     3781 ggcgagcgtt cgtcatattg tattagataa atgcacatta acttatttat ccgaatggaa
     3841 ggaagtacaa caaaatctaa ttcagacaga ctttgtaatg agctataacg gtattcctac
     3901 ccaaaaacat acactatcat atgctattga acgctatgct aagctggctg gtatccatcg
     3961 aatcagaatc catgggttac ggcattctca tgcttccttg ttaatcagta tgggagaaaa
     4021 tccacttatc attaaagatc gtctaggaca cgaagatatt gagacgactt taggaacgta
     4081 tggacatctt tatcctaaca gtaatttcga ggtagctaac aaattaaatg gagccatttc
     4141 ctttaaagaa gcaaaacaaa attatgatac ttcaccaaag aatcaattta ctgtagacta
     4201 cttgcgaaac aaagaaatga aaaaagtgca ataaaagtgc agtaaaaaga taagaagagc
     4261 cagaaaccct tggtataaga ggatttctag ctctcctaca actattccca ctccactgtt
     4321 gctggtggtt tagaagtgat gtcatacacg atacggttga cgtgggctac ttcgtttacg
     4381 atacgtacag agattttttg caagacttcc catgggatac gggcaaagtc agcggtcatt
     4441 ccgtcgattg aagttacagc acggataccg actgtgtagt cgtaagtacg tccgtcaccc
     4501 ataacaccga cagagcggat ccctggaaga acagtgaaat attgccagat atcacggtca
     4561 agtccagcag ctgcgatttc ttcacgcaag atagcatcag attcgcgtac gatttctagc
     4621 ttgtcttcag tgatttcgcc aagaacacga ataccaagac caggaccagg gaatggctga
     4681 cgccaaacga ttgaatcagg catgcctaac tgagtcccta attcacggac ttcgtctttg
     4741 aataatgtgt ttaatggttc gatcaattca aattgcatat cttctggcag tccacctaca
     4801 ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag attcgataac gtcagtatac
     4861 aacgttcctt gtgctaggaa agagacgcct tcttcaccag caagttttgt tgcttcgtca
     4921 tcaaaaacat atacgaattc attcccaatg attttacgtt tttgttctgg gtcagataca
     4981 cctttcaact tgtctaagaa gcgatctctt gcatcgactt tgatgatgtt caagccgaat
     5041 tttccgccta gactttccat tacttgttcc gcttcgcctt tgcgtagcag accgtggtca
     5101 acaaagatag atgtcaattg gtcaccgatc gctttttgta aaagaacccc aacgacacta
     5161 gaatcaacgc cacctgaaag accaagcaag acttttttgt caccaacttg ttcacggatt
     5221 ttttctactt gcatatcgat aaagttatcc attgtccagt taccttcaca gtgacaaaca
     5281 tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg aatgacgtac ttctgcatgg
     5341 aattggatac cataaaaatt acgtgttgtg tcttggatag aagcaatcgg acaatctttg
     5401 cttgttccga ctgtttcaaa gccttcagga actttagtta ccaagtcacc atggctcatc
     5461 catactgttt gttgttttgg tgtttcttta aacaagacag cttgatcgct tgttacttct
     5521 aacaaggctt tcccatattc gcggttttta gcaggctcta cggaaccgcc aagtttgtac
     5581 gtgatcaatt gcataccata gcaaatccct aatacaggaa tacctaaatt gaagatctca
     5641 ggatcgatcc caaatgcatt ctcatcatat acactgtttg gtccgccaga aagaatgatt
     5701 ccctttggat tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa taattcggaa
     5761 aagactccaa gatcacgaat acgtcttgtg atcaattggt tatactgact accgtagtct
     5821 aatacgataa tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt tattacccct
     5881 atccctttaa attttatctc aaaatagtca aactatttta aaatgctaat attttcgcat
     5941 gaaaatcgaa tcgatgatat gattttcagt tttatggaga attatatcgg ctcttcctcg
     6001 tgtaggcaag atatattcct gtaaattttt caaattcaca tttttccata catcttttgc
     6061 cattttaaag gcttcttcac gatctccgat agcaaaacga taataataat tctctggatt
     6121 ttggaaagcc gtatccagta acgcaccaaa acgatccaaa taccattttt cgatcagcac
     6181 gggatcggca tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt
     6241 cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag atatcaggct gctggatcaa
     6301 ttcgtacttg tctggaatga tatcatacac atcatgtgag taaacaggta cttttatctc
     6361 ttcctgacca gattttacac ggttcaagaa atcgatcagc atctccatat cataactttc
     6421 aggaaatcct ttacggtcca tgattccacg ctcttttaat atacgatttg gataaagaaa
     6481 tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg
     6541 cagcagtctg gctgttgtac tttttccaac ggctacactt ccggcgatcc cgataataaa
     6601 aggcggtact tctacgtatt tatgaagaaa taaaccttta ctcaagaaca aagattcaaa
     6661 ttctttcata taaagataaa tcagatgagc aagaggtaca tagatatctc gaacatcctg
     6721 catggaaatg cggtcattca aacttttgat attatctagt tcatcttgtg tcaaaggtgc
     6781 ttgcccatta cgataaaatt ctcgccattc ttctcttgcc acacggtagt agttcattgg
     6841 atcattcata ttagcctcct gcgttctccc atcaatggaa ctattttaac acaaaataaa
     6901 cgcaaacccc accacttaag agagattgcc gatctttttg tataatcttt ataatagaaa
     6961 gaaacggagt cttattcttg acttgcgcac ggatacataa aaggatagac tgttgtcatg
     7021 aaagaaatga gaaggatgga agacatgaca aatcattact attctgaacg cccagatacg
     7081 gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca gaacctttca atttgtaaca
     7141 gattctggtg tattttcacg tgaaacagtg gattatggtt cacgtgtcct gattgatgct
     7201 ttcgattggg aagagttacc tgatgaagga aagatcttag atgtgggatg cgggtatgga
     7261 ccgattgggt tagctttggc atttgcgaca cagcggtttg ttgaaatggt cgacattaat
     7321 gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta accaaatcga acatgttgat
     7381 atccatcaat cgaatatcta tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc
     7441 aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct
     7501 cggttgaaga agggcggtac actgacaatt gtgatccaga agaaacaagg agcgccgagt
     7561 gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa agataaagga
     7621 tattatatca tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag cgacgaaaga
     7681 gtggaactgg gcgtgcatgt acgggtacgt atcagttctt tttttgttga gaagttttta
     7741 aaaacaaaga actatttttt cttcaaaaag aaatagtagg aacagagtcg ttgtttttta
     7801 cgataaaata aataagatac agaacttgta tagaaggctg ccaagtcggt agataaagga
     7861 ggtttttatc aaaaaacata ctttcctttt ttctctattc ttttcttggg ggttttggta
     7921 cactgatagg gaatttagat agaagggagc caataggatg aagctgacca tcagagatat
     7981 cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag attttgaata acaaaggaag
     8041 cagatttagt gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa
     8101 gcccgattat ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg gaatgatcgt
     8161 ccctgatgtc actgatttct tcttctccaa actgatcgaa ggtgtagaga gctatttgaa
     8221 tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa
     8281 gtatttacaa gagctttccc accgctcagt ggacggaatc ttgctggcca caccaaatat
     8341 tttacctgag aaatatgcat tagacagcgg tttttatcaa aaaatgccaa tcattttaat
     8401 cgatcgcgga ttgaatcgtc gggataacgg acggcttatc gtaaaggaat atgagggcgc
     8461 ctatcaagct gtttcgctgt ttattgaaaa tggccatacg aagatcggca tgctgaagga
     8521 aaccactgga tactatcaat tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga
     8581 tcatggcctg ccttttaatg ggaaatttgt tgaacatgga gagttgactg tccaaggtgg
     8641 atatactgca tcaaaaaaat tactcaaaca taaagatatc acggctattt tttgcgggaa
     8701 tgatgccatg gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga
     8761 agatatctca gtcataggat tcgatggatt aaagctgtca gaatacatga ttccgcaatt
     8821 gacgacagtg cagcagccaa gttttgatat tggattttat gctgcacgtt tcctgatcga
     8881 tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat
     8941 tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc
     9001 atttacaaag gctagttagt gcttttttta tttttagtaa accagtttac taaagtggtt
     9061 tacctttcct aaaaataatg tataataccg aatgaagaca aggaggatca ggcaatgcga
     9121 atggtggatc tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag
     9181 tacatcgtca cgaactatac gaatggaaag attccagatt atcaagtcag tgcattattg
     9241 atggcaattt tttatcaaga catgacaaat gaagaaatca cgaacttaac attggcgatt
     9301 gctaattctg gtgatgtcat cgatttgagt tctcttgaag gaataaaggt agataagcat
     9361 tcgacaggtg gcgttggtga cacgaccaca ttgattttag cccccctagt agctagtgtc
     9421 ggagtgacag tggctaaaat gtctggacgc ggtctaggtt acacaggtgg tacactagac
     9481 aagctagaag caattcccgg atttcagatc gagctttcgg atgaagaatt tgtacgcatt
     9541 gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa acttggcacc ggcggacaaa
     9601 aaattgtatg ccttgcgtga cgtcacagcg accgttgatt ctcttccgct gattgccagt
     9661 tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga tgtgaccact
     9721 ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg
     9781 agcattggga aactggctaa ccgagaaaca gtggcggtta tctcagatat gtcagaacct
     9841 cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac attacaagga
     9901 aatggtccag aagatctagt agaaatgtgc tacgccttag gaagccaaat ggttgttctt
     9961 gctggcaaag ctaagacaac cgatgaagca agaacgttgc ttcaagaagc tctggaatca
    10021 ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc aagggggaga cccagcgatc
    10081 gtggaacatc cagaacgaat tttgactgca cgctacacaa tggagcttcc tgctaaacaa
    10141 agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt
    10201 gctgggcgta aaaccaaaga agaagatatt gatcacgctg ttggattgaa actgcataaa
    10261 aaaattggtg acactgtgac aaaaggtgag tcactattga cgatctatag taacgacgaa
    10321 gaaatcactt ctgtcattga attattatat aaaaatattg aaattggtga atcggcaatg
    10381 aagccaacgt tgattcatga catcatcaca gaataaagga aaaaagcgga ctttaccgtt
    10441 ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga tcgaccatac tattttgaaa
    10501 gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt
    10561 tattctgtct gcatcaatcc agcatgggtt gcgctagcga aagaacaatt acaaggtgag
    10621 ccagtagccg tatgtacagt aatcgggttt cctctaggag caaacacacc cgagacaaaa
    10681 gcatttgaaa caacagatgc catcaataac ggtgcagatg aagtcgatat ggtcatcaat
    10741 atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga agcagttgtt
    10801 gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt attagataga
    10861 gaagaaatca tccgagcttg cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca
    10921 tcaactggtt tctcaacagg cggagcaaag gtagaggatg tccgtttgat gcgtgaaact
    10981 gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga agaagcaatg
    11041 gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg caggagtagc aatcgtatct
    11101 ggttcaactg gtgaaggcta ttaatttttt caaaggagaa gctcatggca gaactcaaac
    11161 aagaatggat caacacagct attgaagcgt tagataaagc ctatgttcct tattcccatt
    11221 ttccggtagg cgcttgtctc gtaacggaat caggcaagat ttatcaaggc atcaacattg
    11281 agaatgcatc atttggatta acgaattgtg ccgagcgcac agcttttttc aaagcagtat
    11341 ctgaaggaga aaaaacattt acgcatctag tcgtagcggg gcacaccccc gatcctatct
    11401 ctccttgtgg tgcatgtcga caagtaatgg cagaattctg ttcccctgat atgccggtta
    11461 cgctagtagg agacaatggt gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg
    11521 catttactga aaaagatcta taactcatgt tcattcagtc agctgactgt tgaaaaataa
    11581 aacaattatt ttaggaggct ttacagaaat gaaaaaagca aaattatttg gtttaggtgc
    11641 tgtcgcacta gcagcagggt tatttttagg tgcatgcggc aacaacggct ctactgattc
    11701 atccggtggc aaaagctcat cagacacaac aactgcagca ttgatcacag acacaggtgg
    11761 tgtagacgac cgttcattca accagtcagc ttgggaagga ctagaaaaat gggggaaaga
    11821 tcaaggactt tcacgaggaa atgatggctt ccaatatttc caatcttcaa atgaatcaga
    11881 ttacatccca aatattgacc aagcattgaa tgctggattc aaaacgattt ttggtattgg
    11941 ttataaacta aaaccagcga tccaagaaca agcaacaaac aatacaggta cgaactttgt
    12001 tattatcgat gatgtcatcg atggattaga taacgttgta tctgcaactt tcaaagacaa
    12061 cgaagcttct tatcttgcag gtgttgcagc agcttatacg actgaaacaa acgtagtcgg
    12121 ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg
    12181 tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc aatatgctgg
    12241 tgacttctct gctccagata aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc
    12301 agatatcatc ttccatgctt caggcggtac aggtaacggt gtattccaag aagcaaaatc
    12361 attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg accaatcaga
    12421 cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc acattgacat ctacactgaa
    12481 agcagttgga acagtagtag aagatttggc acaaaaatca gcggacggta aattccctgg
    12541 cggcgaacac actgtttatg gattgaaaga agacggtgtt ggtctaacag aaggacaact
    12601 ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt caggtgacgt
    12661 aaaagttcca gaaactcctg aagaaaacta aaaccatttc tatagccttg ttagacacat
    12721 gtttgacaag gctatggttt gattagaaca agaattgttc atttatccaa gctgccttgt
    12781 gacagtttgg ataagggaac aactccctga agtaatgttt gtttcaaaag aagaacgctc
    12841 actaagcgtg aatcttagtc aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa
    12901 aggtggttca agaaaatatt gtcatagaaa tgcagaatat taccaaagaa tttggaacat
    12961 tcaaggcaaa tgacaacatc aatttacaag taaaggcagg agaaattcat gcgttgcttg
    13021 gagaaaacgg tgctggcaaa tctacattga tgaacgtgct ttccggatta ttagagccga
    13081 catcagggaa aattttgatg cgtgggaaag aagtaaagat cacaagtccg accaaagcca
    13141 accaattagg gattgggatg gtccatcagc actttatgct tgttgatgcc tttactgtaa
    13201 cagaaaacat cgtgttggga agcgagccta gtcgcgcagg gatgcttgac cataaaaaag
    13261 cacgaaaaga gatccaaaaa gtttctgaac agtatggatt gtcagtcaac ccagatgctt
    13321 acgttcgtga tatttcagtt ggaatggaac aacgggtaga aattttaaaa acactttacc
    13381 gaggagcaga tgtactgatt tttgatgagc cgacagctgt attgacccct caagaaattg
    13441 atgaattaat cgtgatcatg aaggaattag tcaaagaagg caagtcgatc attttgatta
    13501 cgcataagtt agatgaaatc aaagcagtag ctgaccgctg tacagttatc cgccgtggaa
    13561 aaggaatcgg tacagtcaac gttaaagacg taacctcaca acaattagcc gatatgatgg
    13621 ttggaagaac ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt
    13681 tgtctattga aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga
    13741 acttagaggt tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga aacgggcagt
    13801 cggagttgat ccaagctttg actggtttgc gaaaggcaga aagcggacat atcaagctaa
    13861 aaggggaaga catcactaat aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg
    13921 tgccagaaga ccgtcataaa tacgggttag tcctagatat gacattatct gaaaatatcg
    13981 cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat tattcagtaa
    14041 tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg
    14101 ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg
    14161 accgagatcc tgatctgttg attgttgcaa atccaactcg tggactggat gtaggtgcga
    14221 tcgaatttat tcataaacgt ctgatcgaac aaagggacaa atacaaagca gtgttactga
    14281 tcagtttcga attagaagaa attttaaatg tttcggatcg tattgctgtt atccatgaag
    14341 gagaaattgt cggtatcgtt gatccgaaag aaacatctga aaatgaatta ggtctattga
    14401 tggcgggcta taccttagaa gaagcacgaa aagagttaga aaagggggct ggcgcacatg
    14461 aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt tgttttagga
    14521 ttgatcttag gcgcgatatt gatggctgct tttggttttc agcccatcca aggttattct
    14581 tctatgctag atgcagccct tggttcgcaa agagggatag gtgaaacgtt aaggcaagca
    14641 acgccgttga tttttacagc attaggtttt tctgtagcta actcagctgg gtttttcaat
    14701 attgggcttt ctggacaagc actttgcggg tggatcgtca gtgtctgggt ggcacttgcg
    14761 tttccagatc tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg tgccttagct
    14821 ggtgcgatgg cagcggctgt tccaggttta cttcgagcat tttttggaac aagtgaagtg
    14881 atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa gcacatttgc tttacatgaa
    14941 gtgatgcctg aaagctatcg ctcaagtttg gactcttcca atatgatcag tcaaaatgcc
    15001 tcactgcggt taccatggtt aacacaagct tttggcggct ctcgcgtaaa ctcaggatta
    15061 tttttagcaa tcatcgcgct tattttcgtt tggctgctga tgaagaaaac gactttagga
    15121 tttgaaatcc gttctgttgg attgaatcct tttgcttctg agtatgcagg aatgagtagt
    15181 aaacgtacga tcgttttgtc gatggtcatt tctggcgctt tggcagggct tggcggtgtg
    15241 gttgaaggat taggaacgta tcaaaacttc tttgttcaaa cgacttcgct atctattgga
    15301 tttgacggaa tggctgtttc cttgcttggt tctggatcag caatcggtat tcttttatca
    15361 gcgatcttgt tcagtatttt gaagatcggt ggacttggca tgcagacagg agcaggtgtt
    15421 ccgtttgaga tcgtcaatgt atcgattgct ttgattatct tctttgtcgg gatcaacttc
    15481 ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa aacaagaaga gatcgttgcg
    15541 acaatagaat cgaaaccaac gaatcaaaac catgaagggg gcgaactata atgtcgacta
    15601 tcgaattaat tgccttgatc cttacttcta cactggttta ttcaacaccg ctaattctaa
    15661 cgtcactagg cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa
    15721 tcatggtcat gggtgctttt agttccatcg ttttcaactt gacgatgagt ccatcacttg
    15781 gttctatgac accatggatc gctattttag tgggtggcgt cgtaggtatc ctattttctc
    15841 tgattcacgc agtagcaaca gtcagtttgc gtgctgacca tattatcagc gggacagtaa
    15901 ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc
    15961 aaactgataa catcacgcag aatctggggt atttttcttt cccaggatta gcagatattc
    16021 cggtcattgg accaattttc tttaaaaata cattcttacc tgcttattgt gctattttga
    16081 ttgctgtcct agcttgggtg atcatcttca agacgaaatt cggcttgcgc ctccgttcag
    16141 ttggtgaaaa tcctcaagct gccgacacat taggtatcaa tgtatatgct atgcgttatt
    16201 caggcgtact gatttctggc ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga
    16261 tttccggtaa cttttcagct ggtacgatcg ttggacaagg gttcatctcc atggctgcta
    16321 tgatctttgg taagtggaat cctttagggg caatgggtgc ctcattgttc tttggttttg
    16381 ctcaaagctt gagtatcatt ggcgcacaat taccagttat ttcttcgatt ccgccagtat
    16441 tgctgcaaat tgctccatat ttgctgacaa tcattgtgct ggttgttttc ttagggaaag
    16501 cttctggtcc aaaagcaaac gggaagaact atatcaaatc aaaataatct tttagcgaat
    16561 aaaaagggtg ggttacggga agtgtttttc gtctcgccct ttttgtttta aaggcaataa
    16621 aaataaaaag aaagaaggaa taatcatgtt taaacgcgta catctgatcg tcatggattc
    16681 agtaggaatt ggagaagcac cagatgccga taagttcggt gatgttggtt ctgatacatt
    16741 aggacacatt gcgaaagaag caggattgaa cgttccacat ttagagcagc ttggtttagg
    16801 aacgatccgt cctcttgaag gtgtggaatc agtgaaagat catcaaggct atgcgacgaa
    16861 attagaagaa gtttctgtag ggaaagatac gatgacaggc cactgggaaa tcatgggatt
    16921 gaatatcaaa actccatttc gtgtgtttcc taatggattc cctgatgatc tgatcaaaca
    16981 aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt
    17041 tattgacgat tacggtgaac accaaatgaa aacaggcgat ttaattgtat atacttctgc
    17101 cgatcctgtc cttcagatcg cggcacatga agaaatcatt cctttagaag aattatatcg
    17161 gatttgccaa ttcgttcgtg atataacaaa agatgatccg tatatgattg gccgaatcat
    17221 tgcacgtccg tacgtgggtg aaccaggaga cttcacacga acaagcaatc gccatgatta
    17281 tgcgttgaat ccattcggac acacagtcct tgattctttg aaagaagcag gaaaggacgt
    17341 gattgctgtt ggaaagatca acgatatttt caacggacaa ggaatcactg aatctgtccg
    17401 cacgaaaagc aatatggatg gcgttgacca gctgctgaaa gttatgaaaa acgatttttc
    17461 aggaatcagc tttaccaatt tagtagattt tgatgcatta tacggtcatc gtcgggatgt
    17521 agtcggctat gcgcatgcga ttgaagaatt tgacttacga attccagaat tgcttgaagc
    17581 gatggaagaa gacgacttgc taatgattac agccgatcac ggaaacgatc cgacgtttac
    17641 tggtaccgat catacgcgag aatatgtacc gttattggtt tacagtaaaa aaatgaaaga
    17701 acaaggggca ttgcctcaag gatattattc agatatttca gcaacgattg ctgagaactt
    17761 tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat aatcttttta
    17821 aataggagaa agcaaatgac caagttaagc gagatgttac aagaaacgac ccagtttatc
    17881 cttgataaag gagtaaaaga aattgatttt ggtttgattc taggttcagg attaggtgaa
    17941 ttagcagatg aaatcgaaga tgctgtaatg atttcttatg atgagatccc gttctttcct
    18001 gtttcgacag tagttgggca tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa
    18061 gtgttagcca tgcaagggag attccactac tatgaaggtc attccatgca aacagtgacc
    18121 tatccagtac gtgtgatggc cggactaggg gcccattcag tcattgtgac taatgcatgt
    18181 ggtggtgtca acgaatcatt tgtaccagga gatttgatgc tgattaccga tcatatcaat
    18241 tttactggtg aaaatccttt gatcggtccg aatgaagatg caatgggccc tagatttcca
    18301 gatatgagtc aagcttatac gcctgactat caagcaacag caaaagcagc agcagaaaaa
    18361 ctaggtttgg atcttaaaga aggcgtatat atgggctatt ctggaccaac ttatgaaacg
    18421 cctgcggaga tccgaatgtc tcggacaata ggagccgatg cagtagggat gtcgactgtt
    18481 ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc ttgtattacc
    18541 aacttagcag caggtatgca agcgaatctg agccatgaag aagttgtaga aacaactcag
    18601 agagtcaaac aatcttttaa agcattggtt aaagaaacat tgacattatt gtaataggag
    18661 gaacaataaa atgagtgtgc atattgaagc gaaagaaggc gagatcgccg ataagatttt
    18721 acttccggga gatcctttgc gagctaaata tattgcggag acttttttgg aagatccagt
    18781 ttgttacaat caggtacgcg gtatgttagg ctttaccggc aaatataaag gagaacgtat
    18841 ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc atctatgccc atgaattgat
    18901 ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga
    18961 cgtccatgtt cgtgatttag tactagcaca aggtgcagcg accagttctt cgatgatcga
    19021 aaaaaatttc caagcctttc attttcctcc aatcagtgat ttcaatctat tactgaaagc
    19081 atatgagata gctaaagaaa agggctatac ggttcatgta ggaaatgttc tttcggaaga
    19141 ttctttctac aaagatgatt tgacagagac gttccaatta gctgaacttg gcgttttagg
    19201 agtggaaatg gaagcagcgg ctctttatta cttaggcgcg aaataccatg tgcagacatt
    19261 aagtttgatg accgttagcg accatttgat caccggtgaa gaaacaacag ctgcagaacg
    19321 tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa acagcgatag cagaataaga
    19381 aggaaatgaa aaagctgccc tgcattaagt aataagaaca tccaagcaaa aatagctcct
    19441 catgtttttg acttggctgg acttattatt gcaaatgtaa gcttttgaac accgtttacc
    19501 aagaggttgt gaatctgccg tttaactctg agtcacaagg aacaatttca aaaaacagct
    19561 tttcatgttt gatgaatatt gtgacttgtg accgagaagt tggcaggaga aaaccgttta
    19621 tacgaaaaaa gggactgtga caaatcgtgt cacagtccct tttttatagt gttttttata
    19681 ggtagtattt tttagcaact gttaagtcat catttaattc ataaacaagt ggttgacctg
    19741 ttggaatttc aagatccatg atgtcttcgt cagagatacc ttcgatatgt ttagctaggg
    19801 cgcgtaatga gttaccatgt gcagctacta aaacagtttt gttgtctagt aatgctggag
    19861 caatttcatc ttgccagaat ggcaaagcgc gttctaatgt cactttcaag ttttctccac
    19921 caggaatatc gcgtttgtct aacattgcgt aacgacggtc atttgctgca gaaccttcgt
    19981 cagtttcttc cattaatgga ggaagagtat cgtaagaacg acgccagatg tgcacttgtt
    20041 cgtcgccgta tttttcagct gtttcttttt tgttcaaacc ttgtaattta ccatagtgac
    20101 gttcgtttaa gcgccatgat ttgatttgtg gtacccaaag ttgatctgaa tattctaaga
    20161 tcaagttaca tgttttgatt gcacgagtta aaacagaagt gtaagcaaca tcaaattcga
    20221 ttccagcttc tttgattttg cgtccgcctt cttttgcttc ttcgattcct tctggtgcta
    20281 gatttacatc agcccaacca gtaaattggt tcaatgcatt ccattcacta agaccgtgac
    20341 gagaaaaaac taattttggc atgtttagct ctccttttaa gataggttta tatttctgaa
    20401 attttcagtt ctgttttatt gtacaatttt ttggaagaga tttccatcga aaaattagat
    20461 aagattctat gagaaaacaa gtagagaata taaaaaaacg aacagtgccc acgcaactgt
    20521 tcgtttttag gtattagatg cataattgct cgatttcttt ttcaggtaaa ttgatatatt
    20581 tataacatgt tccaactgaa acaccgcatt ttgaagaaat aaattggatc gtttcttttt
    20641 tttcataata gagacgtcgg atttttttga tcgtttttgc atcgattttt ggtcttccgc
    20701 cgatttttcc ttttttacga gcttcatcta atccaactaa tgtacgttct ttgatcaatg
    20761 cgcactccat gtttgctagg ttttccatta attggaaata gatttttccc atttcgcgcg
    20821 tatcgattcc ttcatcaaga ctaatcaagt gcagcccgcg atctttgaat aattgcgtga
    20881 gttctgttaa ttgccgcgta gatttgccta atcgatgaag ttggcaaatg actagtgcat
    20941 cgccttgttg catgtcagcg atcactgact ccaaatgaac agcagtagaa tcattttgtt
    21001 ccacttcata tgtctcatgg aaaatttctt gacaaccaaa gtcagataat tgatcaacct
    21061 gagttgttaa atcaacgtca tttatggttg tgcgtgcata accaataatt ttcatggtga
    21121 ttcctttcta gcagtgattt tctaactgga tataacagta aaaatgtagc ataaagtacg
    21181 tcaaaaagat agttttttcg caaattttcc gctgtctttt tttattggat gatcaagcag
    21241 tttttcttta tttacgacgg attgcgtcaa gaatctcaac gcctttttct gagccgacaa
    21301 ctactttaga aaccatgttt aggaaaagcc catgttctac taccccaaca agttcatcta
    21361 aataggtagc taagctttct ggctgttcga tgacttctag atgcagatca atgatgtaat
    21421 gaccaccatc tgtggttaag acttctccgt tttcgtctgt acgcaagacg ggttggaatc
    21481 ttttttcttc aaaaatatga agtagctgtt gggagccata agggatcact tcaactggga
    21541 gtgggaactt cccaagttta tcgactaatt tagaagaatc aacgatccaa atcgtttctt
    21601 ttgagtacgt agcgactatt ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt
    21661 gaaaatcagc actgatttca tcagcaccgt ctattgtcaa atcgactacg gggacttcat
    21721 caatactctt taacggtatt cctaattttt ccgcttgttc ttttgtcgca tttgaagtag
    21781 taactcctgt gatacgtaaa ccttcttcac gcatacggcg accaatctct tctaccatat
    21841 aataggcggt tgaaccggtc ccgagaccta cgatcatccc gtcctttacg aatttcgcgg
    21901 cttcaatgcc agctaattgt tttaaattca tctttgcgtc cacctttttt tagtttgagc
    21961 atcgtgcttt cctcactatt ttagcgtgat ttacggtaaa aagataccat tcttttttat
    22021 tccaataaag ctttttcaaa aaaaattgca tctcttgttg acatgttttt tcattcgtga
    22081 tattctaaca aaggtgcttt atctacaggt ttctgtgaag aaacgtgctg actgtttatc
    22141 aaagcatatt tgataaggat gcagacattg catttatttt ttatcacaaa aaaagaacca
    22201 cctggatgtg tggaactaga cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat
    22261 taaccaatta gtacgtaaac ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt
    22321 gaacaaagga tataacagct ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg
    22381 tggggtagca actcgtgtgg gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa
    22441 atatgcacgt gttcgtttgt caaacttaat tgaagttact gcttatatcc cagggattgg
    22501 tcataactta caagaacaca gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc
    22561 aggggtacgt taccatatcg ttcgcggtgc tttagatact gctggtgtta acgaccgtaa
    22621 acaaagccgt tctaaatacg gaactaaaaa acctaaagct taattttaat tattaaacaa
    22681 atgaaacttg cttctactat atagaagtaa ttactatgaa caatatttcg gaaggaggag
    22741 ttacggatgc cacgtaaagg tcctgttgca aaacgtgatg ttttaccaga tcctatttat
    22801 aactcaaaat tagtaactcg cttgatcaac cgtgtaatgg ttgacggaaa acgcgggatt
    22861 gcagctaaca ttatctataa tgcctttgac ttgatcaaag aatctacagg taacgatcca
    22921 ttagaagtgt ttgaacaagc aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt
    22981 cgtgttgggg gttcaaacta ccaagtacca gttgaagttc gccctgaacg ccgcgttact
    23041 ctaggtcttc gttgggtagt aaactacgct cgcttgcgcg gtgaacacac aatggaagaa
    23101 cgcttagcaa aagaaatcat ggatgctgca aacaacacag gtgcttctgt taaaaaacgt
    23161 gaagacactc ataaaatggc tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat
    23221 tctctctgtt tggtcggctt ttataagtcg acctcagata gtgtccttat ttttagcaac
    23281 tagatagtaa agagaggaga aacaaggaga atgaccagag aattttcttt acaaaacact
    23341 cgtaatatcg gtatcatggc ccacatcgat gctggtaaaa cgactgctac tgaacgtatc
    23401 ttgtactata ctggtaaaat ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg
    23461 gactggatgg aacaagaaca agaacgtggt attacgatca cttctgctgc tactacagca
    23521 caatggaagg gtcaccgaat caacatcatc gacacaccag gacacgtaga cttcacagtt
    23581 gaagttgaac gttcattgcg tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca
    23641 ggtgttgaac ctcaaactga aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt
    23701 atcgtatttg caaacaaaat ggacaagatc ggtgctgact tcttatattc agtatcaact
    23761 ttacatgacc gtttgcaagc aaacgctcac ccaatccaat taccaattgg tgcggaagac
    23821 gacttcacag gaattatcga ccttgtaaca atgaaggctg aaatgtatac aaacgatctt
    23881 ggaacagaga tcgaagaaac agaaattcct gaagaatacc gcgaattagc tgaagaatgg
    23941 cgcgaaaaat tggttgaagc tgtagctgaa actgatgaag aacttacttt gaaatactta
    24001 gaaggtgaag aaatcactga agctgagtta aaagaaggta tccgccgcgc aacagttaac
    24061 gtagaattct acccagtact atgtggttca gcatttaaga acaaaggggt tcaattacta
    24121 ttggacgctg ttcttgatta cttgccatca ccgttagata tcccagctat caaagggatt
    24181 gaccctaaaa ctgatgaaga agtagaacgt cctgcagacg acagcgctcc tttctcagct
    24241 ttagcattta aagttatgac tgacccattc gtaggtcgtt taactttctt ccgtgtgtac
    24301 tctggtgttt tacaatcagg ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt
    24361 gttggtcgta tcctacagat gcatgccaac tctcgttcag aaatctctga agtttacgct
    24421 ggagatatcg ctgctgctgt tggtttgaaa gacacaacta ctggggacac actatgtgat
    24481 gaaaagagtc tagttattct tgaatccatg gaattcccag aaccagttat ccaagtcgct
    24541 attgaaccta aatcaaaagc tgaccaagat aaaatgggtg tggctttaca aaaactatct
    24601 gaagaagatc ctactttccg tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct
    24661 ggtatgggtg aacttcactt agatatcatc gttgaccgta tgcgtcgtga attcaaagtt
    24721 gaagctaacg taggtgcgcc tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa
    24781 gccgaaggta agtttgtacg tcagtctggt ggtaaaggtc aatacggtca cgtatggatt
    24841 gaatttacac caaatgaaga aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg
    24901 gttgttcctc gtgaatacat tccagctgtt gaaactggtt tgaaagatgc tatggaaaat
    24961 ggtgtcttag ctggctatcc attagtagat atcaaagcta aactttacga tggttcatac
    25021 catgatgtcg attcaaatga aactgccttc cgtgttgctg cttctatggc attacgtgca
    25081 gcagctaaga aagctaatcc agtaatcctt gaaccaatca tggctgtaga agttgttatc
    25141 cctgaagatt atttaggtga tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa
    25201 ggtatggaag ctcgcgctgg cggccaacaa gttgttcgtg cgatggttcc acttgctgaa
    25261 atgtttggtt atgctacaac tcttcgttct gctactcaag gtcgtggtac atttacaatg
    25321 acatttgacc actacgaaga tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac
    25381 ggcggaaaag ctgaataatt tcttaaaacc ggatagtcct gcaaagagat tatccaaaga
    25441 gactatattt ttaatatagt ttcgtgtaaa ataattatcg atgatatggg catgtggtgt
    25501 accttgtgta catcatatac ctatcagaca aataaaaaat atattgtatt tttaggagga
    25561 cattttaaaa tggcaaaaga aaaatttgac cgttctaaac cacacgttaa cattggtact
    25621 atcggacacg ttgaccatgg taaaactaca ttaacagctg caatcacaac tgtactatct
    25681 aagaaaaacg gcggccaagc tatggcttac gatcaaatcg atggtgctcc agaagaacgc
    25741 gaacgcggaa tcacaatctc aacagctcac gttgagtatg aaacagatac acgccactac
    25801 gctcacgttg actgcccagg acacgcggat tacgttaaaa acatgatcac tggtgctgct
    25861 caaatggacg gagctatctt ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt
    25921 gaacacatcc tattgtctcg tcaagttggt gttccttaca tcgttgtatt cttgaacaaa
    25981 gtagacatgg ttgatgacga agaattacta gaattagttg aaatggaagt tcgtgaccta
    26041 ttaacagaat acgaattccc tggtgacgat gttcctgtag ttgctggatc agctttgaaa
    26101 gctctagaag gcgacgcttc atacgaagaa aaaattcttg aattaatggc tgcagttgac
    26161 gaatacatcc caactccaga acgtgacaac gacaaaccat tcatgatgcc agttgaagac
    26221 gtgttctcaa ttactggacg tggtactgtt gctacaggtc gtgttgaacg tggacaagtt
    26281 cgcgttggtg acgaagttga agttgttggt attgctgaag aaacttcaaa aacaacagtt
    26341 actggtgttg aaatgttccg taaattgtta gactacgctg aagctggaga caacattggt
    26401 gctttactac gtggtgttgc acgtgaagac atccaacgtg gacaagtttt agctaaacca
    26461 ggtacaatca cacctcatac aaaattctct gcagaagtat acgtgttgac aaaagaagaa
    26521 ggtggacgtc atactccatt cttcactaac taccgtccac aattctactt ccgtacaact
    26581 gacgtaacag gtgttgttga attaccagaa ggaactgaaa tggttatgcc tggcgacaac
    26641 gtaacaatgg aagttgaatt aatccaccca atcgctatcg aaaacggtac taaattctca
    26701 atccgtgaag gcggacgtac agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc
    26761 acttaagttt taaagtaaag caacatttat tcggacgtaa tcgctcggaa cgaaggtctt
    26821 aggatctttg ttccgagctt ttttatttat tgcaggatca ggaaaaaaga caggagtatt
    26881 ataaatagaa gaaacacgaa ataaaagagt cagtattttc taaatgaaac tttatgaaaa
    26941 caaaatgttt tttacaagaa aaagccttgc tttgtgagtt tatatatatt ataatgacaa
    27001 aggtgcgttt actttagtga attgcacaga cgtaatcaga atgatttcgt cggctaagaa
    27061 acgagaggtt gcgacacgcc cggacgcttt gccacgaacg agcgtgacgg aaattttcgt
    27121 ggagctatgt ctacttttca aaataggcga aggagggaac aagatggcaa aacaaaaaat
    27181 tcgtatccgt ttaaaagcgt atgaacaccg tattttagat caatcagcgg ataaaatcgt
    27241 ggaaactgca aaaagaactg gagctagcgt gtctggtcca attccattac caactgaacg
    27301 cagtctttac acaatcatcc gtgcgactca taaatacaaa gattcacgcg aacaattcga
    27361 aatgcgtaca cacaaacgtc ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc
    27421 tttgatgaag ctagacttac catctggtgt aaacatcgaa atcaaattat aaaactaaaa
    27481 caaaaataat ggaggtgtac tcatgaccaa aggaatctta gggaaaaaag tgggaatgac
    27541 acaaatcttc actgaatctg gtgaattaat tccagtaact gttgtagaag ctactccaaa
    27601 cgtagtacta caagtgaaaa caatggaaac ggatggctac gaagctattc aagttggtta
    27661 ccaagatatg cgtgaagttt tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa
    27721 cacggctcct aagcgcttca ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt
    27781 aggaaaagaa attacagtag atgttttcca agcaggagac attattgatg taacaggtac
    27841 tacgaaaggt aaaggattcc aaggggttat caaacgccac ggacaaagcc gcggaccaat
    27901 ggctcacggt tctcgttacc atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg
    27961 tgtatttaaa aacaaacgtc ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa
    28021 cttggaagtt gtacgtgtcg atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc
    28081 tggagctaaa aaatctttaa ttactatcaa atcagctgtg aaagctaaat aattgtagga
    28141 agaaaggagg aactaaggaa tgccgaatgt agcattattc aaacaagatg gaagccaaaa
    28201 cggtgaaatc acacttaacg aagaaatctt cggaattgaa cctaatgaaa gtgttgttta
    28261 cgatgcaatc gtaatgcaac gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg
    28321 tagcgctgtt cgcggcggcg gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg
    28381 tcaaggatct atccgctcac cacaatggcg tggaggtggc gtagtctttg gaccaacacc
    28441 acgttcttac agctacaaac ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt
    28501 gtcagaaaaa gttgctgaaa acaacttggt agcaatcgaa ggcttgaact tcgacgcacc
    28561 aaaaacaaaa gaattcaaac aagttcttgc taacttatct atcgactcta aagtattggt
    28621 tgttctagaa aaaggtaacg actttgctgc tttatcagca cgtaacttac caaacgtttc
    28681 tgtagtaact tctgataatg tgagtgtgct agacgttgta tcaaatacaa aagtgttagc
    28741 aacacaaact gctcttactc aaattgagga ggtgcttgca taatgaactt actagacgta
    28801 atcaaacgcc cagtgatcac tgaaaaatca atgcttgcca tggatgacaa gaaatacact
    28861 tttgaagtgg acactcgcgc aaacaaaact ttagtaaaac aagcggtaga agctgctttt
    28921 gacgttaaag tcaaaaacgt gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc
    28981 aaatatgcag gatatacaaa aaaacgtcgc aaagcgatcg ttacattgac tgaagattca
    29041 aaagaaatcc aattattcga agctgctgaa taagcactaa tagataactc attaaattag
    29101 gagggaaaaa cgtggcgatt aaaaagtaca aacctacctc aaatggtcgt cgtaacatga
    29161 caggttctga ttttgctgaa atcacaacat caacacctga aaaatcattg ttacaaccat
    29221 tgaaaaacca tgctggacgt aacaacaacg gccgcatcac tgtacgtcat caaggtggcg
    29281 gacacaaacg ccaatatcgc gtgatcgact tcaaacgtaa caaagataac gttgttgcaa
    29341 ctgttcaaac gatcgaatat gatccaaacc gttcagctaa catcgcgtta gtacactatg
    29401 aagatggagt gaaagcttac atcttagcac ctaaaggact agaagtgggt atgaaacttg
    29461 tttcaggtcc agaagcagat atcaaagtcg gaaatgctct tccattagaa aacattccag
    29521 ttggtactgt tatccacaac attgaaatga aacctggtaa aggtggacaa ttgatccgtt
    29581 ctgctggtac aagtgctcaa gtacttggta aagaaggcaa atacgtattg atccgcttga
    29641 actctggtga agttcgtatg atcttagctg catgccgtgc tacaatcggt tctgtaggta
    29701 acgaacaaca cgaattaatc aacatcggta aagctggacg ctctcgttgg atgcgtaaac
    29761 gcccaactgt acgtggtagc gtaatgaacc ctaatgatca cccacacggt ggtggtgaag
    29821 gtaaagctcc tatcggacgt aaagcaccag tttctccttg gggtcaacca gctcttggtt
    29881 acaaaactcg taacaaaaaa gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata
    29941 aataattact ttgaccatgt gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt
    30001 taaagaaagg accttttgtc gatgagcatt taatgaaaaa agttgaagct caagcagggg
    30061 ttgaaaagaa aaaagttatc aaaacttggt cacgccgttc aacagttttc ccaagttttg
    30121 ttggatatac catcgcagtt tatgatggac gtaaacatgt accagtttac atccaagaag
    30181 acatggtagg acacaaatta ggtgaatttg cacctacaag aacttatcgt ggacatgctg
    30241 ctgacgataa gaaaacgaaa cgctaatttg aggggaggat aaatccaaat ggcagaacaa
    30301 atcacatcag ctaaagcaac tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta
    30361 gtaattgacc ttatcagagg taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca
    30421 ccaaacaaat ctgctggaat cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa
    30481 aataactttg acttagatgt tgaaaacttg gtagtaagcg aagcttttgt taacgaagga
    30541 cctacaatga aacgtttccg tcctcgtgca aaaggatcag cttctccaat caacaaacgt
    30601 acaagtcata tcacggtagt cgtatcagaa aaataaggag ggataatcag tgggtcaaaa
    30661 agtacatcca attggaatgc gtgtaggcat catccgcgac tgggatgcta aatggtatgc
    30721 tgaaaaagag tatgctgagt tcttacacga agatttaaga atccgtaaat ttatttcaac
    30781 taaacttgct gatgctgctg tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa
    30841 catttcaatt cacacagcta aaccaggtat ggttattggt aaaggcggtt ctgaagtcga
    30901 aagcctaaga aaagaattaa acaaattaac tggtaaaaga atccatatca atatcgtgga
    30961 aatcaaaaaa ccagatctag atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga
    31021 aaaccgtgtt gctttccgtc gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg
    31081 cgctcaaggg atcaaaactc aagtatctgg tcgtctaaac ggagcagata tcgctcgttc
    31141 agaaggatac tcagaaggaa ctgttcctct tcatacttta cgtgcggaca ttgattacgc
    31201 atgggaagaa gcagatacaa catacggaaa actaggagtt aaagtgtgga tctatcgtgg
    31261 agaaattctt ccaactaaaa aaaacactga gaaaggaggg aaataatcat gttagtacct
    31321 aaacgtgtaa aacaccgtcg tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga
    31381 aaagaagtag cattcggtga atacggtttg caagctgttg attcacattg gatcacaaac
    31441 cgccaaatcg aagctgctcg tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta
    31501 tggattaaaa ttttccctca caaatcttat actgccaaag caattggggt acgtatgggt
    31561 tctggtaaag gggcacctga aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt
    31621 gaaatcgcag gcgttcctga agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa
    31681 ttaccaatga aaactaagat cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt
    31741 taaagaaatc agagaattaa ccactgccga aatgcttgat caagaaaaac aattaaaaga
    31801 agaattgttt aatcttagat tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat
    31861 taaagaagta cgtaaatcga ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta
    31921 attatggaag gaggccatta agcgtatgac tgaaggaaga aatcaacgca aagtttatca
    31981 aggtcgcgtg gtttcagaca aaatggataa aacaattacc gttgctgtcg aaacaaagaa
    32041 aaaccaccct atctatggca aacgcatgaa ctattctaag aaatacaaag tgcatgatga
    32101 aaacaacaca gcaaaagttg gcgacatcgt gagaattatg gaaactcgtc cattatcagc
    32161 tacaaaacgt ttccgtttgt tggaagtagt cgaagaagca gttattatct aataacacga
    32221 gattttccta tatagattga atgcaaaatc tgaaaggagg atacacaacg tgatccaagc
    32281 agaaagtcgt ttaaaagtcg ctgataactc aggcgcacgt gaaattttga cgatcaaagt
    32341 ccttggtgga tctggtcgta aaactgctaa catcggtgat gtgatcgttg ctacggttaa
    32401 acaagcaacg ccaggtgggg ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg
    32461 aacaaaatca ggagctcgtc gtacagacgg ttcatatatt aaatttgatg aaaatgctgc
    32521 agtgattatt cgtgatgata aaagccctcg tggaacacgt atctttgggc cagttgcacg
    32581 tgaactacgt gaaaacaact tcatgaagat cgtttcacta gcaccagaag ttttataatc
    32641 ttgagacact tatcaaagga ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt
    32701 taaagttatc actggaaaag acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa
    32761 aaaagataaa gttattgtcg aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca
    32821 agctgctcct caaggtggaa ttgttgagat ggaagcgcca attcatgttt ctaacgtgat
    32881 ggtggttgac tctactggtg tagctggccg agttggctac aaagaagtag acggcaaaaa
    32941 agtccgtgtt tctaaaaaaa ccggtgaagt tttagataaa taaaagttta aggaaggagg
    33001 gtcttactga atgaaccgcc taaaagaaaa atatcttaaa gaagtaactc catcattgat
    33061 ggaaaaattt aactatagct ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa
    33121 catgggtgtt ggtgatgcgg tatcaaacgc taaaaactta gataaagcag ttgaagaatt
    33181 aactttgatc actggtcaaa aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt
    33241 ccgtttacgt gaaggaatgc caattggtgc aaaagttaca ttgcgcggag aaagaatgta
    33301 cgaattctta gacaaattgg tttctgtttc actaccacgt gtacgtgact tccatggtgt
    33361 aagtaaaaaa gcctttgacg gtcgtggaaa ctatacttta ggtattaaag aacaattgat
    33421 cttcccagaa gtagattatg atttagtaga taaagtacgt ggtatggaca tcgttattgt
    33481 aacaacagca aactctgatg aagaatctcg tgagttgttg acacaattag gcatgccatt
    33541 ccaaaaataa ttaaaggagg cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa
    33601 acgtcctgca aaattttcaa cacaagctta tactcgttgc gaacgttgcg gacgtccaca
    33661 ttcagtttat cgtaagttcc atctttgccg tatttgcttc cgcgaacttg cctataaagg
    33721 tcaaattccc ggcgtgaaga aagctagctg gtaaaaagta aattcgcata agaaggaggt
    33781 aaaagatcaa tggtcatgac agatccaatt gcagattttc taactcgtat ccgtaacgca
    33841 aacatggtta aacatgagtc tttagaagta cctgcatcaa aaattaaacg tgacatcgct
    33901 gaaatcttga aacgtgaagg tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa
    33961 ggcgtgatcc gtgttttcct aaaatttggt aaaaacggcg aacgtgttat cacaaactta
    34021 aaacgtatct ctaaaccagg tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta
    34081 ttgaacggtt taggtatcgc aatcatttcg acttctgaag gtgttgtcac tgataaagaa
    34141 gctcgtgaaa aaaatatcgg cggcgaagtt atcgcttacg tatggtaatc aagtagaatc
    34201 tacaaggagg tgtctttaag tgagccgtat cggaaataaa gtcgttgtta tccctgaagg
    34261 agtaacagtt actcaagacg gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac
    34321 tcgtactttt tcagcagata tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg
    34381 tccaaacgac agtaaagaaa tgaaaacaat tcatggaaca actcgtgcga acttcaacaa
    34441 catggttgta ggtgtcagcg aaggtttcca aaaagcgtta gaactaatcg gggttgggta
    34501 ccgtgcacaa ttacaaggat caaaacttgt attgaacgtt ggttactcac atccagtaga
    34561 aatcacacca ccagctggag taactgttga agtaccagct aacacacaag taattgttaa
    34621 aggtgcgaac aaagaagaag ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc
    34681 agaaccttat aaaggtaaag gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg
    34741 taaaactggt aaataatcgt ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca
    34801 tttggctgaa gtttaccttc aaaaaaatac atttttactt gtgccgcagc ttctattttt
    34861 cgaaacgtat ttactgcttg aaatagtagt acaataaaaa taaagaggtg acaattgtga
    34921 ttacaaaacc agataagaac aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata
    34981 tctctggtac tgctgagcgc ccacgcttga acgtttttcg ttctaacaaa aacatctacg
    35041 ctcaaattat tgatgacgta gcgggtgtaa cgctagcaag tgcctctacc ttggataaag
    35101 aaatttcagg tggaacaaaa acagaagcag cacaagctgt cggtaaacta gttgctgaac
    35161 gtgcagctga aaaaggcatt aaagtagtag tctttgaccg tggtggatac ctttaccatg
    35221 gccgtgtgca agctttagct gaagctgctc gcgaaaatgg actagaattt tagaagaagg
    35281 aggaatacca ttcatggttt atatcgatcc aaaacatttg gaattagaag accgcgttgt
    35341 tgcaattaac cgtgtaacaa aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt
    35401 agttgttgtc ggtgacaaaa acggacacgt agggtttggt acaggtaaag ctcaagaagt
    35461 tcctgaagca atccgtaaag caattgaaga tgcgaagaaa aacttagttg aagtacctat
    35521 ggttggttca acaatcccac acgaagtaat cggtgctttt agtggtggta gaatcctaat
    35581 gaaacctgct gtagaaggtt ctggggttgc tgctggtgga ccagttcgtg ccgtattaga
    35641 attagctggg gtagcagata tcacttctaa atctttaggc tcaaatacac caatcaacgt
    35701 tgttcgcgca acagttgaag gtttgaaaca attgaaacgt gcagaagaag ttgcagaact
    35761 tcgcggcaaa tcagttgaag aattaatcgg ataaggagga caacaataat ggctgaatta
    35821 aaagttactt taaaacgcag tattatcgga cgtcctcaaa accaacgcga tacagttaaa
    35881 gcgttaggtc ttacaaagat taacagttca gtggttaagc cttctaatga agcaattaaa
    35941 ggcatgatca atactgtttc tcacctagtg gacgttgaag aaatttaatt tagaaaaatc
    36001 ataactagaa cattaaggag gtgccaaacc tatgaaactt catgaattaa aacctgctga
    36061 aggttcacgc caagtacgta accgtgttgg tcgtggtact tcatctggta acggaaaaac
    36121 tgccggacgc ggacaaaaag gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt
    36181 tgaaggggga caaacaccat tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa
    36241 ccgtaaagac tatgcagtca ttaatcttga tgtattgaac cgctttgaag acggagctga
    36301 agtaactcct gttgctttag ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa
    36361 agtattagct aatggggaac taaacaagaa attaactgtg aaagcagcaa aattctctaa
    36421 agcagctcaa gaagcaattg aagcggctgg tggatcaatc gaggtgatct aatgtttaga
    36481 ctattaaaag atgcttttaa agtaaaagac attagatcga aaatcctatt tactgtattt
    36541 gtgttgtttg tatttcgcct aggtgcacat attactgtac caggcgtaaa tgctgcttca
    36601 ttgcaaaacc tttcttcttt gccatttttc aacatgttga acttggtaag tggtagtgcg
    36661 atgcaaaact tttccatttt ctcaatgggg gtttcaccat acattactgc ttctatcgtc
    36721 attcagcttc ttcaaatgga catcgtgcct cgatttgttg aatggtcgaa acaaggggaa
    36781 gttggtcgta aaaaactgaa tcaagcgact cgatatctga cattagtatt ggcatttatc
    36841 caatctgtcg gtatcactgc tggattcaat caatacgctc aattaggttt cgtgaataat
    36901 cctaacgtag caacttacgt aacgatcggt ttgattctaa cagccggtac gatgtttgtt
    36961 acttggctag gagaacagat cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt
    37021 tttgcaggta tcatttctag attgcctgaa agtatcagag aaatttatga agattacttt
    37081 ataaatattg atccttcaga catatggaaa tctgctattt ttgtggccat tttactagta
    37141 gcggtcttgt tgatcgtgac attcgtaaca tttttccaac aagctgaacg caagatcccg
    37201 atccaatata cgaaacgcgt agctggcgcg cctacaagca gctatctgcc gttgaaagta
    37261 aatgcagctg gagttattcc agttatcttt gctagttcgt ttattgctac gccaaatgct
    37321 gttttacaag cattgagcgg tagattcggt tctgaaaact ggtacaaagt gatgatggag
    37381 atctttaact ataatacagc acctggtgct acgatttata cgattctgat cgttgccttc
    37441 actttcttct acgcctttgt tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag
    37501 caaggaagtt atataccaag cgtacgccct ggtaagggga ctgaagagta tgtctcaaga
    37561 ttactgatgc gtttaagtac agttggtgca gtcttccttg gtttagtggc attgcttcca
    37621 atcattgctc aaatggtttg ggatcttcca caatcaatcg gattaggcgg tacaagctta
    37681 ctgatcgtta tcggtgtagc tttagaaact gcgaaacagt tagaaggctt aatgctgaaa
    37741 cgtaaatatg ttggattcat tgagtaagaa agcgtgttga ggtttcccct caacacacgt
    37801 tctcacacat ttattaggag ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc
    37861 aggtaaagga acacaagctg aaaaaatcat tgatgcttat ggtattccgc acatctcaac
    37921 aggagatatg ttccgcgcag cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc
    37981 ttatatggat aaaggtgcat tagttcctga tgaagtaaca aatggaatcg tgaaagagcg
    38041 actagctgaa ccagatacag agaaaggctt cttattagat ggctttccac gtacgctaga
    38101 tcaagcaaaa gctctggacg caatgctaaa agatttgaat aaaaaaattg atgctgtcat
    38161 cgatatccat gttggtgaag aaatcttggt cgaacgtcta gccggccgat ttatctgcag
    38221 caattgtgga gcaacttacc ataaaatttt caaccctaca aaagttgaag acacatgtga
    38281 tcgttgcggt gggcatgaat tctatcaaag agaagatgat aaacctgaga cggttaaaaa
    38341 tcgtctggct atcaacatca aaaatagtga accaatcttg gcttattata aagaacaagg
    38401 tttgctaaac actattgatg gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa
    38461 aatcattgaa aaatagcacg attgcccgta gccgacttgc attatgcagt tactcttgtc
    38521 aagttcaata aattatgata gaatatgtca gtccgacttc tagagaatcc tctagatgat
    38581 ccattttgag aattttgagg tgggaactgc agtttccgcc attttatcgt gtgaaaatgc
    38641 gaaacaagta caaggaggta ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta
    38701 cagtcgtcga aactttgccg aatgcaatgt ttaaagttga actagaaaac ggacaccaag
    38761 ttcttgctac tgtttcaggt aaaattcgta tgcattacat ccgtatctta cctggtgaca
    38821 aagtaacggt tgaattgtct ccatacgatc ttaaccgtgg ccgtattact tatcgcttta
    38881 aataattgta ctccgtaaaa ctacaggagg tattatcatg aaagtaagac catcagtaaa
    38941 accaatgtgc gaacattgta aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc
    39001 agcaaatcca aaacataaac aacgtcaagg ataattggag gtgctataaa tatggctcgt
    39061 attgcaggag tagatattcc tcgtgataaa catgtagtag tttcacttac ttatatctat
    39121 ggtattggta acactactgc taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt
    39181 cgcgttcgtg aattaacgaa tgaacaaaca gatgctatcc gtgcagaagt agataaatta
    39241 aaagttgaag gggatcttcg tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc
    39301 ggttcttacc gtggaatccg tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa
    39361 aacaatgcac gtactcgtaa aggcccagct cgcgctatcg ctggcaagaa aaaataattc
    39421 ttaagtgaag gaggttaaaa cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg
    39481 aagaagaata tagaagcagg tattgcacat atccattcta cattcaataa cactatcatc
    39541 atgatcactg atactcacgg aaatgcatta gcgtggtcat cagctggttc attaggtttc
    39601 aaaggaagca aaaaatcaac tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa
    39661 gcagcaatgg aacacggatt aaagactgtt gaagtaacag ttaaaggacc tggttctgga
    39721 cgtgaagcag caattcgttc attacaagct acaggtttag aagtgactgc aattcgtgac
    39781 gtgactccag ttcctcataa tggtgcccgc cctccaaaac gccgtcgtgt ttaatgagtg
    39841 ccgctcattt tgagttttat cagtaacgtc attgaacaag acacttttcg ttttgaaagg
    39901 ggtaaagata agaatgattg agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa
    39961 agattatggc aagttcatcg ttgaacctct tgaaagaggg tatgggacta ctttagggaa
    40021 ttccctacgt cgtattttat tatcttcttt gccaggtgct gccatcacta atattcaaat
    40081 cgatggtgta ctgcatgaat tctctaccat caaaggtgtt agagaagacg tcactcaaat
    40141 catcttgaac attaaaggct tagcgctgaa aatgtatggc caagaagaaa aaacccttga
    40201 aatcgatatt accggacctg ctacagtaac tgctggcgat attatcgttg atagtgatgt
    40261 agaaattctt aacaaagata tgtacatctg tagtgtctct gaaggagcta cgttccatgc
    40321 tcgcttaaca gtgaaacctg gacgtggtta tgttcaagca gatgaaaata aaaaagaaga
    40381 tatgccaatt ggcgttcttc cagtcgattc aatctacaca cctgttcgtc gtgtgaacta
    40441 tcaagttgag aacacacgtg ttggacaccg tgaagatttt gataaattga cgatggagat
    40501 ttggacagat ggttcaattg aaccgttgga agcaatgagt ttgtctgcga aaatcatgac
    40561 tgaacatttg gatatttttg tgaacttaac tgatgaagca aaaaatgctg aaatcatggt
    40621 cgaaaaagaa gaaacacaaa aagaaaaaat gttggaaatg acgatcgaag agttagactt
    40681 gtctgttcgc tcttacaatt gtttgaaacg tgcaggaatc aatactgtac aagaacttac
    40741 aaataaatct gagcctgaaa tgatcaaagt ccgtaactta ggacgtaaat cattagaaga
    40801 agttaaagca aaattgcacg acttaggttt aggtttacgt aaagaagatt aaaacctttt
    40861 acgaaggagg gaaaccaacg tgagttatcg taaactagga cgcacatcta gccaacgtaa
    40921 agcgatgttg cgtgatttaa caactgattt attaatcaac gaacgtattg tgactactga
    40981 agctcgtgcg aaagaagttc gctcaactgc agaaaaaatg atcactttag gtaaacgcgg
    41041 agatttgcat gcacgtcgtc aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg
    41101 tgaagaaaat gaggacatcg tcattgaatc agctttacaa aaactgttca acgatattgc
    41161 acctcgttac gcagaacgtc aaggcggata cacacgcatt ttgaagacag aaccaagacg
    41221 cggtgatggt gcaccaatgg ttgtcttaga acttgtctaa aatcacacta ataacatcac
    41281 tttatagatg attcttttag agtgttatga tgttggaaga tatttttccg agtctagctc
    41341 agcgctgctc ctcaagcgaa tatcgcttgg ggagtaagca ctcatcataa agtgtttttt
    41401 tataccttca attacctgtc atgacagata tgtttgaagg cataaaaaaa cattttaagc
    41461 gagaaataat tgaaaatttt gtcttttttt gatagtgtta gtaggaactt taggatgaag
    41521 aaggagtaat catggagcca attattgaat tagaaaagat aaattataaa taccagccag
    41581 atgatcttcg ccccgcattg aaagatgtct cctttacaat tgataaaggt gagtggatcg
    41641 ctattattgg acacaatggt tcaggaaaat cgacacttgc caaaacgatc aatggattgc
    41701 ttctgccaga atcagggatc gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt
    41761 ggacgatccg acagatggtt ggaatgattt ttcaaaatcc agacaatcag tttgttggtt
    41821 cgacagtaga agatgacgtt gcttttggtc ttgaaaatca agggattccc agagaagaaa
    41881 tgctagtgcg tgtgaaagat gcacttgaga aagttagaat ggctgagttt gcttcacgcg
    41941 agcctgctcg tttatcagga gggcaaaaac aacgtgttgc cattgccggc gtggttgcct
    42001 tacgacctga tatcattatt ttagatgaag caaccagtat gctagatcca gaaggtcgtg
    42061 aagaagtgat ctctacgatc aagaagatca aagaagaaag tcaattgacc gttatatcta
    42121 tcactcatga tattgatgaa gcagctaatg ccaatcggat actagtgatg agacaaggcg
    42181 aacttgtccg tgaaggtaca ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact
    42241 taggtctaga tctaccattt cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg
    42301 atgtgcccag tgaatatatg acagaagaaa ggatggtgga ttggttatgg acatccgttt
    42361 tgaacaagta gacttcactt atcagccgaa tacaccattt gaacaaagag ccttatttga
    42421 catcaatatg acgatcaaag aaaacagtta tactgcatta gtagggcata ccggaagcgg
    42481 gaaatcaacc ttacttcagc atttgaatgc gcttgttaag ccaacaagcg gtacagtcca
    42541 tattggagaa cgagatattc agccggatac ggataataag aatttaaagc ccatccgaaa
    42601 aaaagttggc atcgtctttc agtttccaga agcacagcta ttcgaagaaa cggtagcaaa
    42661 agacattgct ttcggtccta aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc
    42721 aaaagaaaca ttagaacaag ttgggctgga tgaaagctat ttggaacgtt cgccatttga
    42781 actttcagga gggcaaatgc gtagagtagc aatcgctggt gtgcttgcca tgaggccgga
    42841 agtacttgta ttggatgaac caacggcagg acttgatccc cagggcagaa aagaaatgat
    42901 ggagatgttt tggcgtcttc ataaggaaca gcagatcact attgtattag ttacgcattt
    42961 aatggatgat gtagccaatt ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt
    43021 taatagcggg gagccgcaag aggtctttca aaacattgaa tggctaaaag aaaaacaact
    43081 aggcgtacct actgccaccg agtttgcaga agaattaatg gctaaaggga tgaactttgc
    43141 taccttgcct ttgacagctg aagaattagc agacgctatc gtccattatg ctggaggaaa
    43201 tgcccatgat gaataaattg atatttggtc gttatatgcc tggagattcc ttgatccatc
    43261 gattggatcc ccgagctaaa ttattagcca gtttttattt tatcggaatc atttttttag
    43321 caaataattg ggaaacgtac cttttgttgg catttttcac attactttct gtcttcttgt
    43381 caaaagtaga tttgaaattt tttatccgag gcattcgccc attgatctgg ctaatcctct
    43441 tcactgtagc tttgcaggtc ttgtttacaa gtggcggaga aacttattgg agttggggtg
    43501 ttttccatat cactgaattt ggtgtgatga acggcctctt tattttctgt cgattcgtgt
    43561 tgatcatttt tatgtcgact ctactcacgc ttactacacc gcctttggaa ttatcggatg
    43621 cgattgagta cttgcttcgt ccattgaaag ccgtacggtt cccagtccac gaaatttcat
    43681 tgatgctgtc gattgctctt cgttttgtgc ctacattgat ggacgaaaca gaaaagatca
    43741 tgaacgctca gcgcgctcgc ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga
    43801 aagcagtcat tcctttgctg attcctttat ttgtcagcag cttcaaccgt gcggaagatc
    43861 ttgcgactgc tatggaagca agaggctaca aaggtgggga aggaagaacg aaatatcgtg
    43921 tattgcattg gcataataga gacacgatgg ttatcattgc ttttgtatta ttaactgtgc
    43981 tactgatctt tttaagagga taaacgttca gtgggccgct atctattctt ttcttgctga
    44041 tctgtttgca agaaaggatg caagcagtgt atgacaagaa atattagggt ctgtggcaga
    44101 ctaaggtcac aggcctcttt cttttatctg aaaagaaaga acgaaaaaat tttatagaaa
    44161 aatcattatt ccagataacc ttttaggaac aaaggagaga aaaacatggt acgttacaaa
    44221 gcaatcattg cgtacgacgg aacaaatttt aatggattcc aaaaacagcc aaatgggcgt
    44281 actgttcaag aagaagtaga aaaaacatta caaaaaatgg caaatggaaa agaaatcacg
    44341 gtctttggtt cgggaaggac agatgccgga gtacatgcga tcgggcaagt aatccatttt
    44401 gattatccag aggagcgtcc gttagaacga atgcgtttcg cgctagatac ccagtcacca
    44461 gaggatattg ctgtaagaca agtagaaatt gtttcagaag acttccacgc aagatatttg
    44521 gtcaaagaaa agacgtatca atttcgagtg gatataggga aaccacgcag tccttttcga
    44581 cgccattatg caagttattt tccttaccca cttgatttgg aaaaaattca gcgggcgctt
    44641 cctgatttat tagggactca cgatttcacc tctttttgtg catcaggaag ctcgatagag
    44701 gataaaatac gtacgatcta cgaggctaag atggaggtaa atgaaacaaa agacgaattg
    44761 ttgtttacat ttaggggaaa tggcttcttg tataagatga tacggatctt agtaggaacg
    44821 cttttgaaaa ttggcaatgg ccgattgcct gaagatagta tcccagatat tattgcgaaa
    44881 aaagatcgta atgcagcagg acctacagca caccctgaag gactttattt gtatgaagtg
    44941 aaatatgaat aagaggttat gatagaagtg gtcagcttca agaaataaga aggtatttac
    45001 gaaaattact tctcaaaatt ttgcaaaatt tcggcttatt ttcgaagaag ctacttctgg
    45061 aacaccgttt aagaaagagg tcgtgagcct gtcatttaac tctgagtcac aaagagcaat
    45121 ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact tgtggacgag gcgttgataa
    45181 gtgaatcatc tttttgttac ctattttttc tgtaaccaaa aaacgatgga acgttgtttt
    45241 gattgccctt ggtgcggaca gtctgaacag gttccatcgt ttctttattt tctacgtctt
    45301 cttctgtgtg tgtttcgtgg ttgttcttct attgctggat caatcgtgcc ataaaagggg
    45361 cgattgcggt caaggtacca gacataaaga ataaacaatg caatacatag gacaaaaagg
    45421 attgcaccta tcgtaaatcc ttcaggcaag taactgaaag tgacagtatg tttacctgct
    45481 ggaactttta tcatcaagaa tgcgtctttg aaattgctga ccttgactgg tttcccatca
    45541 attttagctt tccatccttt gtcataagga atagttgtca ccaacatgga atcttcagcg
    45601 gtatttacag tcccagaagc ttttctgcca gtggcttcca tttcgacacc tttttctttg
    45661 atctgattca ttgcggcttg ataggctaca acatctagac caacgacttt aggctccatg
    45721 aaacttattt ctttcgtccc ataaaaactt gcagtaaatg tcactgttgt ctcttgagga
    45781 taatagccaa gatcatagta ttgaccagaa atgttgattt gcgtttttcg cgatgtacca
    45841 ttgacagtga tcgtcgcact agaactttct aattgcgcaa aattggttgg gaacaaactt
    45901 aagtaagctt gtgtatttgc aggcacatgg acttcccaag tgagctcttt tggctggtta
    45961 tctgctgttt ctgagtaagt gacgccagtc gttgtctttg tgatcgtcgt attcaactga
    46021 ttcttcaaag taggtgtata gaatgtaaaa tactgttcat ttagaccaga caatgtattg
    46081 atcaagtttg tctgactggt caaattatct gtcactggct gctcaacatc attaacggac
    46141 aacggtgcag taaatgctag tggcaatgca ttgacattct gatacagtgt atagtcgccg
    46201 ctttgaccag cttggcggaa gccatattta ctgaacgtgc tttcgttttt agaaatgtta
    46261 tatttgattc ctgtaaaccc atccatcagc aaagtattat tgttataacg gatattcaaa
    46321 tttgtccctc ttgaacggaa acctaattta tccaggtaag aggaagaatt acgatttctt
    46381 atagaagaaa acaaactgat accactgtaa ccatagttga tcgaatcgtt tgcagatact
    46441 ggatccagat tttccaagcg gtaaaacgag tcggaatctt tttttgtttc atttacaaga
    46501 gctttcaagt caggataagg atcagtatat aaactgcggg aagcataatt ccaatcatca
    46561 agaatgccat ggatcatact gttcgtattg accagtgctt cagcagacat gatcaataat
    46621 aacaatatac tcagatgatt gaatgaaagt tttttatgct gataaaatcc cacagcaaaa
    46681 gcataaatcc ctaagaaaac cactgtcaat acaaaggaag tcatcgtcac ataggtgtag
    46741 cttccttcag gtttgatgcc ccatgccacc gcaaagagag cagctacgac caaaatgatt
    46801 cctaacaaca gcccaccatc gtcttgttct atcttttccc atccttttcc tgcaagcagg
    46861 agcactaaga aagagaacag ataggcataa cggaataaaa acatattcgg tgcatgcata
    46921 ccatgccaga acaaattcaa tggaacaaga taaaaacttg cgataagtat ggcgaaaaga
    46981 ctgccgtaca aaaatttatt tttcaacggt actttccgac tggcaaaata aaacagacag
    47041 aggactaatg gtagcagacc tgcgtaaacg aacggaatcg atccgtattt tgtcgtgtca
    47101 taaacaccaa tcatattttt catgattaga tccagataag aagtcgcttc tgttttcaac
    47161 gtagtgatct gactcagtgc ctcgccattt gaccgaagat ccagcactgc tggcaataca
    47221 atgatcattg aagcgccgcc tgccagtaaa gaagtgatac cataaggaac aatcgttttt
    47281 ttatattctg accagtttgt caatagacgg gcgataaaat ataagaaaga aaatacgcca
    47341 atcataaaac ccatataaaa actggataaa aacaagagta aataactgat aaataaaagt
    47401 ttcggccgtt taagatccat aacacgatga atccccaaaa tcactaatgg aagataaaca
    47461 aatgcatcca gccacatgat gatctccgaa tgtgcagtga taaatgagat caatgcgtaa
    47521 ctgacgctaa gtgcaacatg cccccagcga ggaatcttga acgtacgtgc aagaaaccaa
    47581 aaagccaatc cggcacagcc gaccttaagc aaagtcagaa catacagtgc atctggcatc
    47641 atctggtttg gaaaaaagaa caccagtggc gtgaagatcc cgcctagata gtaagaaatt
    47701 aaagataaat aattcaatcc tagagaagca ttccatgtat aaaaaatact ttgctttcca
    47761 agaagaacat ttcggaagct ggcatgaaaa ttagaaaact gtgaaaaggc atcacttgcc
    47821 aaaatacttc tgctgcttcc aggatagatt ccgatactta gataaacaag tgctaaaatc
    47881 aaaaacggaa tcaggaaact agctgctgcg tagcgtccgt tttctctaaa gaatgacttt
    47941 aaattttttt tcatatttat tttccaccaa atttctatga agttgtattc agtttatcat
    48001 atccttgttt ttctctgctt aaa
//
LOCUS       DACDLI010000014        46707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDLI010000014 DACDLI010000000
VERSION     DACDLI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.014"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IV353_000850"
     CDS             591..1280
                     /locus_tag="IV353_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9009121.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IV353_000851"
     CDS             1267..2475
                     /locus_tag="IV353_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9009122.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IV353_000852"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IV353_000852"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9009123.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV353_000853"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV353_000853"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9009124.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IV353_000854"
     CDS             complement(6578..7273)
                     /locus_tag="IV353_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9009125.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV353_000855"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV353_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9009126.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IV353_000856"
     CDS             complement(8472..8663)
                     /locus_tag="IV353_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9009127.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IV353_000857"
     CDS             complement(8684..9574)
                     /locus_tag="IV353_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9009128.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IV353_000858"
     CDS             complement(9561..10328)
                     /locus_tag="IV353_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9009129.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV353_000859"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV353_000859"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9009130.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV353_000860"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV353_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9009131.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV353_000861"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV353_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9009132.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IV353_000862"
     CDS             14944..15282
                     /locus_tag="IV353_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009133.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IV353_000863"
     CDS             complement(15450..16208)
                     /locus_tag="IV353_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9009134.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IV353_000864"
     CDS             complement(16226..17044)
                     /locus_tag="IV353_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9009135.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV353_000865"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV353_000865"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9009136.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV353_000866"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV353_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9009137.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IV353_000867"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IV353_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9009138.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IV353_000868"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IV353_000868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9009139.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IV353_000869"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IV353_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9009140.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IV353_000870"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IV353_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9009141.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IV353_000871"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IV353_000871"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9009142.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IV353_000872"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IV353_000872"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9009143.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IV353_000873"
     CDS             27086..27385
                     /locus_tag="IV353_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9009144.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IV353_000874"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IV353_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9009145.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IV353_000875"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IV353_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9009146.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IV353_000876"
     CDS             28352..30325
                     /locus_tag="IV353_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9009147.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IV353_000877"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IV353_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9009148.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IV353_000878"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IV353_000878"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9009149.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IV353_000879"
     CDS             33204..34496
                     /locus_tag="IV353_000879"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9009150.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="IV353_000880"
     CDS             35232..36095
                     /locus_tag="IV353_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9009151.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="IV353_000881"
     CDS             complement(36224..37846)
                     /locus_tag="IV353_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9009152.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="IV353_000882"
     CDS             38095..38616
                     /locus_tag="IV353_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009153.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IV353_000883"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IV353_000883"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9009154.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="IV353_000884"
     CDS             40697..41515
                     /locus_tag="IV353_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9009155.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="IV353_000885"
     CDS             complement(41618..41815)
                     /locus_tag="IV353_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009156.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="IV353_000886"
     CDS             41984..42562
                     /locus_tag="IV353_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9009157.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43607..44110)
                     /locus_tag="IV353_000887"
     CDS             complement(43607..44110)
                     /locus_tag="IV353_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9009158.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="IV353_000888"
     CDS             44469..45494
                     /locus_tag="IV353_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9009159.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45841..>46707
                     /locus_tag="IV353_000889"
     CDS             45841..>46707
                     /locus_tag="IV353_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009160.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGI"
BASE COUNT        14284 a         8817 c         9181 g        14425 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttatat
    45661 ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct gagatggtca
    45721 atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa gttttatagt
    45781 tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt catttaaaca
    45841 atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa tgaaatccaa
    45901 attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat tgtacaagca
    45961 aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga tcctaataaa
    46021 gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt tgcagcatta
    46081 gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc attgaagatt
    46141 ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca tgaacatttc
    46201 caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta cactagtggc
    46261 ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa agggtacacg
    46321 gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga tgcagattta
    46381 tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga actttatttt
    46441 cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc caatcttact
    46501 aaatggttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg attattatca
    46561 gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga tgcccaaata
    46621 catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt gcttgcaggc
    46681 gagcacattg gctctggctt aggtatt
//
LOCUS       DACDLI010000015        39907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDLI010000015 DACDLI010000000
VERSION     DACDLI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.015"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            78..1442
                     /locus_tag="IV353_000890"
     CDS             78..1442
                     /locus_tag="IV353_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9009161.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            1613..2683
                     /locus_tag="IV353_000891"
     CDS             1613..2683
                     /locus_tag="IV353_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009162.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            2698..3366
                     /locus_tag="IV353_000892"
     CDS             2698..3366
                     /locus_tag="IV353_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009163.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            3386..3964
                     /locus_tag="IV353_000893"
     CDS             3386..3964
                     /locus_tag="IV353_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9009164.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            4367..5278
                     /locus_tag="IV353_000894"
     CDS             4367..5278
                     /locus_tag="IV353_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9009165.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(5534..6451)
                     /locus_tag="IV353_000895"
     CDS             complement(5534..6451)
                     /locus_tag="IV353_000895"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9009166.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            6653..7090
                     /gene="zapA"
                     /locus_tag="IV353_000896"
     CDS             6653..7090
                     /gene="zapA"
                     /locus_tag="IV353_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9009167.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            7148..7696
                     /locus_tag="IV353_000897"
     CDS             7148..7696
                     /locus_tag="IV353_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9009168.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            7811..10171
                     /locus_tag="IV353_000898"
     CDS             7811..10171
                     /locus_tag="IV353_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9009169.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            10258..10572
                     /gene="trxA"
                     /locus_tag="IV353_000899"
     CDS             10258..10572
                     /gene="trxA"
                     /locus_tag="IV353_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9009170.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            10754..12532
                     /gene="uvrC"
                     /locus_tag="IV353_000900"
     CDS             10754..12532
                     /gene="uvrC"
                     /locus_tag="IV353_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9009171.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            12677..13576
                     /locus_tag="IV353_000901"
     CDS             12677..13576
                     /locus_tag="IV353_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009172.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            13569..14831
                     /locus_tag="IV353_000902"
     CDS             13569..14831
                     /locus_tag="IV353_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009173.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            14994..16892
                     /locus_tag="IV353_000903"
     CDS             14994..16892
                     /locus_tag="IV353_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9009174.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(17191..17910)
                     /locus_tag="IV353_000904"
     CDS             complement(17191..17910)
                     /locus_tag="IV353_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9009175.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            18247..18579
                     /locus_tag="IV353_000905"
     CDS             18247..18579
                     /locus_tag="IV353_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009176.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            18566..20569
                     /locus_tag="IV353_000906"
     CDS             18566..20569
                     /locus_tag="IV353_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9009177.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            20588..21058
                     /locus_tag="IV353_000907"
     CDS             20588..21058
                     /locus_tag="IV353_000907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9009178.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            21079..21663
                     /locus_tag="IV353_000908"
     CDS             21079..21663
                     /locus_tag="IV353_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9009179.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            21678..22664
                     /locus_tag="IV353_000909"
     CDS             21678..22664
                     /locus_tag="IV353_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9009180.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            22654..22965
                     /locus_tag="IV353_000910"
     CDS             22654..22965
                     /locus_tag="IV353_000910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9009181.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            22987..24768
                     /locus_tag="IV353_000911"
     CDS             22987..24768
                     /locus_tag="IV353_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9009182.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            24761..26137
                     /locus_tag="IV353_000912"
     CDS             24761..26137
                     /locus_tag="IV353_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9009183.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            26144..26779
                     /locus_tag="IV353_000913"
     CDS             26144..26779
                     /locus_tag="IV353_000913"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9009184.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            26809..28164
                     /locus_tag="IV353_000914"
     CDS             26809..28164
                     /locus_tag="IV353_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9009185.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(28356..29750)
                     /locus_tag="IV353_000915"
     CDS             complement(28356..29750)
                     /locus_tag="IV353_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9009186.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            29842..30813
                     /locus_tag="IV353_000916"
     CDS             29842..30813
                     /locus_tag="IV353_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9009187.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            30810..31592
                     /locus_tag="IV353_000917"
     CDS             30810..31592
                     /locus_tag="IV353_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9009188.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(31761..32132)
                     /locus_tag="IV353_000918"
     CDS             complement(31761..32132)
                     /locus_tag="IV353_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9009189.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            32266..32709
                     /locus_tag="IV353_000919"
     CDS             32266..32709
                     /locus_tag="IV353_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9009190.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(32752..33561)
                     /gene="yidA"
                     /locus_tag="IV353_000920"
     CDS             complement(32752..33561)
                     /gene="yidA"
                     /locus_tag="IV353_000920"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9009191.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(33580..34947)
                     /locus_tag="IV353_000921"
     CDS             complement(33580..34947)
                     /locus_tag="IV353_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9009192.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(34931..35761)
                     /locus_tag="IV353_000922"
     CDS             complement(34931..35761)
                     /locus_tag="IV353_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9009193.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            35924..36361
                     /locus_tag="IV353_000923"
     CDS             35924..36361
                     /locus_tag="IV353_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9009194.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            36437..37048
                     /locus_tag="IV353_000924"
     CDS             36437..37048
                     /locus_tag="IV353_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9009195.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEEEEFSDSEKE"
     gene            37237..37743
                     /locus_tag="IV353_000925"
     CDS             37237..37743
                     /locus_tag="IV353_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9009196.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            37760..38659
                     /locus_tag="IV353_000926"
     CDS             37760..38659
                     /locus_tag="IV353_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9009197.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            38746..39630
                     /locus_tag="IV353_000927"
     CDS             38746..39630
                     /locus_tag="IV353_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9009198.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVHPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            39779..>39907
                     /locus_tag="IV353_000928"
     CDS             39779..>39907
                     /locus_tag="IV353_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9009199.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTD"
BASE COUNT        13195 a         6831 c         8484 g        11397 t
ORIGIN      
        1 aattatgatt tcccctattt gaaccaattt ttaaaagaat gttatttaga aaaattgaat
       61 ttaaggatga ataaaacatg aaaatcgtga tcattggtgc ttcccatgca ggcatcacag
      121 ctgccctaaa tctaagaaaa cttcaaccga aagcagaagt tttactgatc gatgaagatc
      181 ataaagatgg actaggctat gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac
      241 gtagtttagc tgaagttgcc cataacatgc ggactcttca agaaagtggg gcaaaactga
      301 tcaccgaatg gagagtaact gaacttgatc cagacaaaca tcagctcatc ctcgcatcca
      361 aagaaggaaa aaacgagacc ataacctatg ataaattaat cgtagcaaca ggttcctctc
      421 ctgtcacctt gtataaacaa attgaagctg aaaatgtcta cacctataaa aatctagtgc
      481 aatctaagca agtacttgcc gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg
      541 gttatattgg attggaatta gcagatgctt tgagaaataa aggatacatt attcatctag
      601 ttgattatat gccgaatgtt ttatcaagat atttcgataa ggatatgatc aacagttttc
      661 aaaatcagct gcaaactaag caaatcaatt tttatccaaa tgaattcttg atcgattgga
      721 aaaaatcaga agagaaagta gtatccgtac aacttctatc tcaagcaata aaggcagata
      781 tggtcatttt ttctgcccaa actcgtccta atacgacatt gttaaaagaa aaagtggctt
      841 tgtatgagga tgagactgtg atagtcaatg aatatctgca gacaagtgat ccggacatct
      901 atgcgattgg agatatcgtc ccagtcagtt ttgacaaaaa taaacggcat ctctttttac
      961 ctttagttac tcgagctgta catatggcaa gagcagtagc gctaactttg agtggtcagc
     1021 caaccgctta cgacctcaga caaaagataa cagcagcagt cattatagac tactttttag
     1081 gaactgtagg gctgacagaa gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt
     1141 caggagaatt tgatctattt ccacaatacg atgaagaaaa caaaacagta aatgcaaagc
     1201 tgatctacca tccggataca ttagaaataa ttggcggaca gctgataagc caagaattct
     1261 tattatcaga tttgaacctg cttgcggata tcgtgaaaca caaaactacg attccacagc
     1321 tagcagttga ggattttgga tttctgtctg aacacacctc tcgttttcac tacctcaatg
     1381 agttagcttt taaagtacta gctaagagag caaatagcaa tagagtagat aaaagactgt
     1441 gagaaatatc acggtctttt attgtgtggg gatataataa atattaatca atcgagaata
     1501 aaaaattcat ataatgaaat agcggaagaa ttttttgagc ataatattcg ccaaaaattt
     1561 atcaagtgat aaaattgttg tataaaatga ttatttcagg aggaggagtt aaatgttttt
     1621 agcattaaat gaaattatgc attcaaagtt acgttatgcg ttagttgcag gggtgatgtt
     1681 tttgatcgcc tacctggtat tttttttgac tgggttagct tatggcttag ctcaagataa
     1741 tcgaacagca gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc
     1801 aaatctaggg atgtccatga ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt
     1861 ggcgtatctt gcccagacac ctggagtcgt gactagtaaa gacagtacag aagaaggaaa
     1921 aatcaatgtt agcttcttcg gtatcgataa aaatcaattc atcatgccca atcttgttga
     1981 aggaaaagct tttgataacg atgatgaggc agtaggcgat attagtttga aagaagaata
     2041 tggtctagcc gtaggagata cagttaagtt atctggtagt gacaagacgt ttaaactaac
     2101 tggctttacg gatcatgcca aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc
     2161 ttaccaagag atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat
     2221 agttgtcaga ggtaaaatca gtaatttacc tgaagattta gaacagatca aaatttctaa
     2281 attcatcaat gaattacctg gatataatgc gcaagtattg acttttggat ttatgatcgg
     2341 atttttgatc gttattgcgg ctattgtgat cgggattttc atttatgtac tgacgatgca
     2401 aaagattaat atctttggtg taatgaaagc acaagggatc acaggaggat ttattgcgcg
     2461 ttctgtagtg gcacaaacat tcattttatc atttgttggt attcttcttg gtctattagg
     2521 aacagtggga acatcccttg ttctacctga tgcggttcca ttccaaagca attggctgtt
     2581 ttttggcgtc attagcttgt tgatgctggt agtagctgtg ttaggcgcac tattttctgt
     2641 tcggacaatc gtaaaaatcg atccgttgaa ggcaattgga taagaaggag gaagagcatg
     2701 aaagcaatag aatttaaaga agtagaaaaa agtttccaag atggcgacca agtagttaaa
     2761 gcattgaaag aaacaaccat gagtatcgat aaaggggaat ttgttgcaat catcggtcct
     2821 tctggttcag gtaaaagtac ttttttgact ttggcaggcg gattgcagac gcctagcagt
     2881 ggctctgtat ttatcaatga tcaagaattc agtaatcaaa aagaaagtga acgcgttaaa
     2941 ctacgtttta aagaaattgg ctttattttg caagcttcca atctggttcc ttttctgaca
     3001 gtaaaaaagc agttattatt agtagataag atcaaacatg aacatcgtca aaaagaagca
     3061 aaagaattat ttgagcagct tggcgtggat aagttgatca acaagtatcc agaagaacta
     3121 tctggtggag aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt
     3181 attctagcag atgaaccaac tgctagtttg gattcagaaa aggcacgaga agtagtcgat
     3241 atcttggcaa aagaatcaaa agacaaaaat aaagcaacga tcatggttac tcacgataca
     3301 cggctgattg acaaatgcga tagaatattt atgatggaag atggtgtatt gaaagaaaaa
     3361 gaataaccaa aaagcaggtg gaaaaatggt ccatcgatac aacaaagata cacagcaaaa
     3421 aattttgaaa gaagcgactg acttgtttat gtccaaggga tatctcggca cttcaacaag
     3481 agagatcgca caaaaagttg ggatcaccca acccaatctc tatcattatt tcggtgataa
     3541 agaaaaacta tatacggcag ttttagaaaa ccacttaaaa gatgttggaa aagctttgag
     3601 agaaatcgtt caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt
     3661 tttgattgag acacatctgg tcgatttgtt tctgatgctt cacgatcttg aaagcaatct
     3721 ttcaaaggaa acaagagatc atcttttctt tctatggaag aaaaattatc gtgaaccttt
     3781 tgaagaaatc ttttcagcaa accaatctgt gctaagaaat gggatttcaa aagaaatcgc
     3841 agcaaggcat tttttcttag tgttagcacc ttacatcact cggtcgaaaa gacctgtgga
     3901 agaaccgcta aatgtggcgc aattgatcga tttatatttg aatggtgtat taggagaagc
     3961 gtaaagctgt tatataggga gaaaaatagt taggtgaaga agcaagatat gacgaagatc
     4021 gttatatctt gtttttttta tataggtagt ttttcataaa tacaaatttg ttattctctc
     4081 atgattatca gggtatattt ttatttatta tgtcggtgtt ccaggaagtg ttttaattta
     4141 ataacgaaga aattaattgt ttgtagaatg aaggaaacta tttattgggt agatgaatca
     4201 ccacgaatag tcaaaaggag atttcaggat atgccaaaaa tgaaaaaaat cattttagca
     4261 gtaatgttat tttctacggt tatatctttt acagcccctt ttttgcgtac ttaagctgat
     4321 tttaagtttt gacccttagt ctggatctac gaggtgagag tcaagaatga aaaaaataat
     4381 tggagctttt gtatttgcaa tcattatcat tattatctgt tttcaaatag atgtttttgg
     4441 tcaaaaaaag atagttggac aaacggtcag cagtacgaac agtacggtta caaagacaaa
     4501 ggaatcattt gatgatacag aacaagagac attaatcaaa atcggaaaaa atgaccgtac
     4561 actatataca gatgactcgc taactacccc acttgcaact ataaacggcg gagagctggc
     4621 ggagtttctt tctgaaacaa aaaatgctta tcagatcaag acaaatgatg gctatactgg
     4681 atatttagcc ctcgaagatg gaaccaaagt cacaaagaat atacaaacaa aaccggaaga
     4741 gctatctgat gcagtgattg tattagatcc tgggcatggc ggaaatgata ctggggcatt
     4801 aagcaatgat gaacaaacag aagaaaaaga tataacatta agtacagcca taaaagtaaa
     4861 aaaggcgcta gaggatgctg gagcaacggt ttatttgacc catactacag atgaattagt
     4921 acagcttgga gatatatgcg actacagtga agaaaaaaag gcagatgtct ttatcagttt
     4981 acacgcagac tctacggaat atgcgaatga agcaactgga atcacgacat attattacta
     5041 tggacaagag gaaacattag cacagacaat tgctgactcc ttcactgatc tgcctctgga
     5101 ttcaagagga atctccactg ggaactatca agttcttcga gaaaatcttc aaccttctat
     5161 tttaatcgag atgggttata tgaataatga tagtgattta aaggaattag taacagacag
     5221 ttatcagcag cagattgctg caagtttgac acaagcattg accacttatt ttcagtaata
     5281 aataagccca gccaaacaaa aatagctcct catatttttc gtttggccgg gcttattatc
     5341 gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactcc
     5401 gcgtcacaag gagcaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt
     5461 gaccgaggag ttggcatgag aacaccgttt attcggaaca agggactgtg acatgacttt
     5521 tgtcacagtt ccattatttt tttagcagct tcaaagcttt ttctgtattg gcaaaatgaa
     5581 gtttggcata tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt
     5641 ggtcagattt cgttccagct cctgatggaa gggtgattcc agcttcagct gatgctttgt
     5701 gtaactcttc caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat
     5761 attgttctcc tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg
     5821 catatttttt aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt
     5881 ctggctcgat tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc
     5941 gaacggcatt gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt
     6001 tataggggat ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact
     6061 cttttgaatc tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag
     6121 cagcacatac tgttagcgga ccaaaatagc tgccatttcc gacctcatca ctacctaaaa
     6181 cggataattg actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt
     6241 tatcctgtgc ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca
     6301 cttttccgga tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat
     6361 aaggaatctg tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt
     6421 cgtttgttgc tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac
     6481 tataccgcaa ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa
     6541 agtttatcat ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca
     6601 acttcatggt acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca
     6661 cgaaaaaaca cgctacaaag cagtgattgc caaccaaaca tatactatta tcggacggga
     6721 aaccaagcat catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa
     6781 acaattatca ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct
     6841 ttcggatcaa ttgaagaaac aagaaagaat cttggagctg gaagaagaaa cagcagaatt
     6901 aaagaaaaaa atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat
     6961 cgagaacgaa gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa
     7021 tcatgtggaa gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc
     7081 ttctaattaa gatcctttta tcaaaataag aaaaatttaa aaaagttaga agagaaagga
     7141 acgtagcatg ctgagtttgc tgattttatt tttattgcta attgcgtttt tttctggagc
     7201 acgtcgcggg tttgctttgc aaatcatatt cgcgattgga tatgtattgt catttattgc
     7261 tgcccagcat ttttacaaac cattagcaag tcatttggaa ttatacattc catacccagc
     7321 tgtcacccca acatcaaagt tagcattttt tgatcaggta tttgcttttc acctagatga
     7381 agctttttat gcaggaactg cttttctgat tattttgcta attggctggc tgcttacccg
     7441 atttgtgggg gtttttgtcc atggattgac gtatgtgccg atcttgagac aggtagactg
     7501 gattgcagga ggcatcctgt cactaatcat ggcttacgtt actatatttt tgattttgca
     7561 gttacttgcc tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc atagtttcgc
     7621 tcgttttatc gttgagagaa cgccatttct taccaacaag atgtatgagc tttgggtaac
     7681 gaatattttg aattaaggaa aatagtgctg gaaaatattt cgttttttgt acagagtagt
     7741 ttatttaaga aggctgcgta gcgtatgtcc ggcacagctc ttttttcata gtgaaagata
     7801 aggtgataaa atgaataacc gaatccttga aacattggaa tttgaaaaag tcaaacagat
     7861 ggtcagacaa tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt
     7921 gagtgagaaa caaacgatca caaactggct tcaagaaact gaggatggat tgaaagtgca
     7981 acgtctgcga ggcgggatcc ctatacctaa acttgaaaat atccgtcccc atatgaaacg
     8041 gatcgagatc ggagcagact tgaatggagt ggagcttgca caagtttcca gagtattgtc
     8101 tacgacaagc gaattgaatc gtttcattga tgatctttct gacagtgaaa tcgaatttgc
     8161 acgtttgtat atgtgggcag atcagttagt cacgatccct gttttgagcc gccgtttaaa
     8221 ggaagcaatc gatgaagacg gacgcgtgac tgatgacgct tctcctgagt taaagagcat
     8281 aagacagaac attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg
     8341 tgggaaaaat gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg accgctacgt
     8401 tatcccagtc aaacaagaat accgcggtgt atttggcggt gtagtccacg atcaaagtgc
     8461 ttcaggacag actttattta ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg
     8521 tcaataccaa atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac tttctgctga
     8581 acttgttccc catcgtcaag aaatcatcca caatgcttat gtgattggta aaatggatct
     8641 gatgaatgca aaagcacgat ttggtaaaga agtaaaagcc atcgtgccgg gaatcagtga
     8701 agacaatcat gtcgtcttga aacaagcaag acatccactg atcgatcagg aaaaagtagt
     8761 ttctaatgac atcacaatcg gaaaagacta tcaagccatc gtcattacag gacctaacac
     8821 aggtgggaaa acgatcacgt tgaagacttt aggtctgtta caactgatgg ggcaagcagg
     8881 attaccgatt cctgcaggtg aagaaagtca aatcggtatt tttgaggaag tatttgcgga
     8941 tataggagac gaacagtcga ttgagcaaag tctatcgacc ttttcttctc atatgacgaa
     9001 cactgtagat attttatcaa aagtcaatga aaaaagtctg gtattatttg acgagctcgg
     9061 tgcaggaaca gatccgcaag aaggtgctgc tctagcaatc gcaatcttag atgatcttgg
     9121 taaaaagtct gcttatgtga tggcaacaac gcattatcct gaattgaaag tttatggata
     9181 caatcgggca aatacgatta atgccagcat ggagttcgat gtggatacac taagtccaac
     9241 gtatcgtttg ctgattggcg ttcctggacg aagcaatgcg tttgaaatct ctagccgatt
     9301 aggtcttgat acagaagtta ttgatgaagc gaagcagttg atgaatgatg agagccaaga
     9361 tttaaacgaa atgatcacag atttagagaa tcgccggaaa atggcagaga ccgaatatct
     9421 ggaaatgcgt cattttgttt ctgaggcaca agaacttcac gatgatttga aagaagctta
     9481 cagctatttc tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaacga
     9541 agtcgtgtca gaggcggaag aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca
     9601 acagcagatt gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa agacccaatt
     9661 agcaaatcta catcaagaag aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga
     9721 acaaaaaacg ttgaaaccgg gagacgaagt gttggttacg acttacggcc aaagagggac
     9781 gcttttacgt aaaaacggga atcagtggca agtagaaatc ggtatattaa aaatgaatgt
     9841 gtccgaagac gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac gagttatcca
     9901 tgctgtacga tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg
     9961 atacgaagaa gcgctaagtg aggtagatca atatttggat tctgccattc tagctggtta
    10021 tcctcaagtg actattgtcc acggaaaagg aacaggcgca cttcgcaagg gaattactga
    10081 ttatcttaaa aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg
    10141 aaatggtgcg acaattgtta aatttaagta aagcttacta aaggtaagca gatggttcgc
    10201 tatccaagta tagaaatgct atactaatca tagattttaa aaatggaggt ctttgatatg
    10261 tcacaagtaa ttacagataa gacatttaat gaagaaacag ataaaggact tgtattgatt
    10321 gatttttggg caacttggtg tggtccatgt cgtatgcaag caccgatttt ggatcagttg
    10381 gaacaagaat atgatgaaga agaattcaga attgctaaaa tggatgtgga tgaaaatcca
    10441 gaaactcctc aacaattcgg tatcatgagc attccaacat taatgttgaa aaaagatggt
    10501 caagtagtag aaaaagctgt tggtgttcat tcaaaagaac aactacgtca aatgatcgat
    10561 caatatttat aaaaacttac agcaaaaata agctgagggt gggactgaat ccagttccgt
    10621 cctcggtttt tttgcggttg agttaaggtt attcaaatta atattttttg agtataatta
    10681 cgttgtcagg ctggattgtt tttttgacag acatgctata ataactttaa ttgaatatga
    10741 aggggagtac cagatgaacg aacgaatcaa aaacaaatta gctttacttc ctgatcagcc
    10801 cggctgttac ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca aagccaaaat
    10861 attaaaaaat cgtgtccgct catattttac tggcagccat aatacgaaaa cggaacggct
    10921 tgttagtgaa atcgaagact ttgaatacat cgttacagaa tcgaatatcg aagcattgct
    10981 cttagagatc aatctgataa agaaaaatga tcctaaatac aacattatgt taaaggatga
    11041 taaaacctat cctttcctta agataacgaa tgaaaaatat ccccgtttgg tgattactcg
    11101 taaagtacta aaagacaaag cactgtattt tggaccatat cccgatgtcg gtgcggcgaa
    11161 tgagacaaag aagatcctcg atcgattatt tcctctgaga aaatgcaaac ctagtcagac
    11221 gaaggagcct tgtctttact atcatttggg acaatgtctg tgtccttatt atttcgacgt
    11281 agatccgaaa gtctatacga aaatcgttga tgaagtgaaa aagtttttaa acggaggaca
    11341 cgaaaaaatc gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa acatggagtt
    11401 tgaaaaagct gcagaatacc gagaccagat ccgtgccatt gagacaatca tgactcgcca
    11461 aaaaatgaca aatactgatt tactggatcg agatgtattt ggttatgcga ttgacaaagg
    11521 atggatgtgt gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac gagatgtttc
    11581 aatgtttcct ttctataacg aagcagaaga agatttcttg acttacctcg gtcagtttta
    11641 tcaggaaaat gagcatttca ttcctaaaga agtcttgatt cctgatacga tcgacaagcc
    11701 cagtgtagaa gctttgttag ctacgaaagt gctgcaacca aaacgaggag aaaagaaaaa
    11761 attagttgaa ttggcgaata agaatgcctc agttgctttg aatgaacggt ttgatctgat
    11821 tgccagaaaa caggaacgga cgatcggagc agttgaacgt ttaggaaacg cgatgaatat
    11881 cccgacccca atacgtatcg agtcttttga taactctaat atcatgggga cgaatcctgt
    11941 ggctgcgatg gtcgtattca ttgatggaaa accggcaaga aaagaatatc ggaaatataa
    12001 aataaaaaca gtcaacggac cggatgatta tgcatcaatg agagaagtca tctatcgcag
    12061 atattcacga gtgattcgcg aagggttgcc tttacctgat ttgatcttga tcgatggagg
    12121 aaaaggacaa gtagatgtgg caaaagatgt cttagaaaat caattaggaa tcgatattcc
    12181 tattgctggc atggcaaaaa acgataaaca taaaacaaat gagttgctat tcggtataga
    12241 tttggctgta gtaccgcttg agcgaaattc accagagttt ttcttactcc agcgaatcca
    12301 agatgaagtc catcgattcg caatcacttt ccatcggcag ctacgcagta aaaacagttt
    12361 tgcttcccga ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa tgctgcttaa
    12421 agagttcaaa tcgttgaaaa atatcacagc agccacgaca gaagaattac aagaaatcgg
    12481 actgccgaaa aatgtcgcac aaaacgtatt tgataaatta catcaggaat gaggaaaggg
    12541 cactttacaa agaaagtaaa gtgtcctatc attttatgta caacaaaaag agcgtttacg
    12601 ttttgttggt aaataataaa tcgcttacgt ctggtgtcta aggatatatg tgatttcttg
    12661 aagaaggagt gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg ggaactacac
    12721 agctgtcgat catgtctctt tccaaatccc cgatggaaaa atcatggggc taattggcca
    12781 aaacggggct gggaagacaa cgacttttcg tttgatcttg gattttttga cacaagacca
    12841 aggagaagtg ttatggaatg gccatcatct tggggagaaa gattatgatg tcatcggtta
    12901 tcttccggag gaacggggac tttatccgaa agtaacgatt caagaccaac tgctttattt
    12961 tgcagaactt agagggaaat caagaaaaga gattgaacca aaaatcgatt tttggatgga
    13021 aaaatttcag gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta aaggaaatca
    13081 gcaaaaagtc cagctgatcg ccacgttaat ccatgaacct aaattgatca ttttggatga
    13141 gccattcagc gggttagatc cggtaaatgc tgaattgcta aaagatggaa tattggaatt
    13201 gaaaaaacat ggttcatgtg tcattttttc tagccacaat atggataatg tagaaaaaat
    13261 ctgcgatcat ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa aagtacatga
    13321 gattcgtgaa tcatttggcc gtacgaagtt attcttggaa tctggtttaa gccaacagga
    13381 agtagaagaa atcgaaggcg tgaaaaaagt gatccttcat gaagacaata cgttagagat
    13441 taccttagaa gatcctgaag ctgggaaaga aatctttact cgtgcgactc agttcggcta
    13501 cattcctatg tttaaccagc aaccgccaac tttggaagag attttcaaat tgaaagcagg
    13561 tgaaatcgat gcgtaagttt tggattatta ccaaagatgt ctatttaaaa aatgttaagt
    13621 cgatttcttt cttgatcatg atattagtcc cttttatcat gctgggtatc atttatgtag
    13681 caggaaactt tgctcaacaa aatagtgatg tcaataaaat cggcgtattg gctgataatg
    13741 agcaaatcag tcagcaaatg agccaagtca aaacagatga ttttgagttc aaagcagttg
    13801 attctgaaga aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg tatttagtag
    13861 tctcaactga gaatgataag ataaatggga tgctttatag cgaaaattca ttaggacaaa
    13921 gtacacaact actgatacaa caacaactga ctggcttgca atcgatgctt cgagcaagtc
    13981 aattaggcat ttcaccagaa gaagtagcta gcttgagcca acaagcaagt ttgactcgac
    14041 aaaaagtcaa ttttgattca aaaggaaaaa tgacagttgg agaagataac tcgacagtcc
    14101 aatatgtcgt aagttatgta gcaacgattg tcttgttcat tattattttg acgtatgcgc
    14161 agatcattgc acaagagatc gcttctgaaa aaggcacgcg gatcatggaa gtgatcctgt
    14221 ccagtacgac tgcacaaaag catttttacg gaaaactaac aggtgtttta cttgttgcat
    14281 taacgcaaat ggcattatac ggtattattt ttggtatagg atacagtcaa ttcaaagata
    14341 tggaaatggt caaaaatata ttagacggta tctcattgga taacttgatt ggaccattct
    14401 tgtggtattc tcttctattc atgatctttg gcattttgat tttttctgtt ttagcagctt
    14461 tatgtggctc tttggtcaac aaagcagaag atacagcaaa agcaattttg ccagtgactt
    14521 atctatctct aggcggatat atgcttggat tgatactagg agcttcagat ccaaacaata
    14581 tcgttattcg gatcacttcg tatattccat tcttatcttc atatattatg ccggttcgct
    14641 tagcaaatga gacagtcggt atgggtggag cattgatttc cttgttggta cttgtagccg
    14701 taacattctt attgatgatc ttgtctgcaa acatgtacaa atcgaatgta ctcgtgtata
    14761 gcgaaggcgg attatggagt tcattgaaac aatcgatttc aattatgaga aatgaacgaa
    14821 aaaaagcata aaatatagaa aaatatagaa ggaagctgga catttattgt tcccagcttc
    14881 ttttttgtat agacctattt ctattttttt attatattgg atatatatat tcattgaaaa
    14941 tagagcataa caactttata atgttgagta taccaatttt tggaggcgtt ttaatggacc
    15001 gctatttaga tttactttca gagaagttcc ctacaacgga agatgtcatc acggaaatca
    15061 tcaatctcga agcaattttg cagcttccaa aaggaacaga gttatttttg agcgatatac
    15121 atggcgaatt tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa
    15181 agatcaaaga ccttttctct gaacaaatga cggaaacgga gatcagccaa ttcactattt
    15241 ttacggctta tccagaatat gtgttgacga ctgattggta caaaaaacaa gataaatccc
    15301 agctgatcca tcgtttgatc gatcttttac ggtttacgac agtcaaatac actcgatcaa
    15361 aagttcgaaa aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg
    15421 atgatcgtat caacgggaaa aaagactatg taaaaaaaat cattgaacaa cttgtcttga
    15481 tgaaggaaga agaaaaattt ttaacaaaac ttgcgcagac gatccaaaaa tctgtcatcg
    15541 atcatttgca tatcgtgggt gatatttttg atcgaggaac tcaagcagcc aaagtaatgg
    15601 atcgaatcat ggatttttcc tctgtagata tccagtgggg gaatcatgat attttgtgga
    15661 ttggtgcgta ttgcggctct gaggcatgtt tattgacgct tttacggatt gccgcacgct
    15721 acaactattt atttgaatta gagaaagaat atggattaaa tttacgtcca ttattttctt
    15781 ttgcccatca aacctatcag aagaatccag tatttacccc taaaaacagg aaagatctat
    15841 ctgaaagaga gcaagaagaa ttagaaaaga tccatcaagc actttctatc ctccaattca
    15901 aatcagaaca tcagttgttg aatcgacgac ctgaatttaa aatggacgat cgtaaattgt
    15961 tggaaaaaat caactatgag gaatcaacga tcgttttaga aggtcagctc tatgagttga
    16021 aagggacttg ttttcaaacg atccagccag atgatcctaa aaaattcttg gaagaggaac
    16081 aaaaagtgat cgatagtttg atgtattcat tccagcactc gttaagaatg caaaaacaca
    16141 tgacgttttt gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtactgttcc
    16201 atggctgtat acctgtagat gaaaaggggc agctcttagc atttgaatta gaccaatctt
    16261 atcgcggaaa agaactgctg ggattttttg aaaaacagat aactgaaagc gcaaaagatt
    16321 tgactgcaaa tgaagatttg gcgaccgatt tgatttggta tgcgtggagc gggccatttt
    16381 cgcctttatt cggcaagagt aaaatggcaa cttttgagcg ctattttctc acagagcctc
    16441 atacgcacgt cgaaaaaagc aatccctatt atcacttgcg agatgaagag tggttcagtg
    16501 aaaaaatttt ggcagaattc gatgcaaaag aagaaggttc agcaatcatc aatggacaca
    16561 cgccagtaaa agtaaaaaaa ggagagtcac cgatcaaagc aaacggaaaa gcatttgtta
    16621 ttgacggagg tctatccaaa gcttatcaaa aaacaaccgg gattgctggc tactcattac
    16681 tatgcaattc ttacggtttc caaatcgtga cacattatcc gttcttgcct attgaacaac
    16741 aatttgcaaa aaaatctgat caaacgaatg taaaaaaagt gatcgagcag cagctcccac
    16801 gcaaacttaa tcggaatacg acaaaaggaa tggaattaca gcaacagatt actcaactcc
    16861 aaaggctgtt ggattatcga aaggacgatt gagaaaaatg ctagccaaca ttgctcaata
    16921 gactaaaaag cgactgcaac aaaaggcgtg tcgcagtctc cttcggaatt agctcaaaaa
    16981 acggtaaaat attagaagct atttttctgt ttatttctct aatactcagg tcagggcgct
    17041 gatttcacga cctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    17101 atatccaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc agagttgaac
    17161 ggcaggctca cgacctaatc agaaaaacga ttaaaattca ttcacaaaga aatcgaataa
    17221 acgctgttca gatgaatcta cacgatgaga aagttccgga tgccattgga caccaaggat
    17281 tttgtgcata ttatctttac tttggactgc ttcaatgagt ccatcgttgg aaaaggcgat
    17341 ggcttttaac aaaggagaaa gatctttgac tgcctgatga tgataggaat tcacaaagta
    17401 tgaatcaggg agaaggtcac ttaataaact gtccgaaacg attttgactt cgtgagttgc
    17461 aaactgcggg gcagtgggct gttgttcgtg ttttacggac cattttggat ataatgaaag
    17521 atcttgataa agtgttccct ccaacacgac attcaatagt tgaagtcctc ggcagactgc
    17581 aaaaatcggt tttctttgtt tcaatgcttc caagatcaat gcctgttcga attggtctcg
    17641 ttgaatattt gtttccgtca gttttggatg agggtcttcc atgtaaagtt gcggactaac
    17701 atcctgaccg cctgcaagaa tgagtttgtc gattttctca attagttgag gcgctgaatc
    17761 tggagacatg actggcagaa taagaggtaa tccttgagca tctaaaactg catccacaaa
    17821 tccctgcggt gtatagctta cttgatttcc ttggaaagta tctgtcgctc gaatcagttg
    17881 attcccggca atgccgatga ttggtttcat ttttattccc cctaaagtag actaattgta
    17941 acaaggattt aaaaaaattt aaactatgta agacttgccc ctatacttat ctgttcttgc
    18001 tgtgttacta tctgtaaaga gaattttcgt gtgaaattca tgacgatagt tgaaaatatt
    18061 gcttaatttg ttataataaa aagtaagcag aaataaaaag gttgggcgta agcttcaagg
    18121 atctagttct tcaaggggtg agaaggacat tttattgaag gggcctgcct atactaagtt
    18181 atctttagag gtacatactt tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga
    18241 aaggaagtga agcaagtgtt agaagctttg gaacaaatcc gaaaagcaga agaagaaaat
    18301 gagaagcgtg ctcgaaccct acggcagcag ttgaagacct atgcagaaga aaaagagcaa
    18361 gaattggaga taatccaaac gaacagtcgg caacagctga aacagttggt agaagaaaaa
    18421 gaacaattcg aaaagcaaga attagaaaaa gaaaaaatga aattgaaagc agaagcagac
    18481 aaggtgcaag caactttgaa agaccaatat caaaagcatc agagtcaagc aatcggtgca
    18541 atcattgaaa gggtgaaaga aacttatggc cgtcactaag atggaaaaag tgaccctcat
    18601 ttcagataaa agaaatcagg agaggatttt acaagcagta caaggaatcc aaaatgttga
    18661 aatccgggat ctgtatcaaa atagaacaaa caaccagtgg gtagaaaaat attttccaga
    18721 taccacgatt attgataaag aagcaaaatt aagcgcttta ggcaatcggc tgaaagacat
    18781 ccaagaagct atccaattca tacaacacca cggcgatagc cgtcaaaaga aactccattt
    18841 gaaacgtcaa gaactttcct tacaagactt ggaagcaaat tattccgaag aaaatttcat
    18901 gaataaatta gaagaagtcc ttgatttaaa agaaaagtgg gaacagctaa ctgaaaaacg
    18961 tgatcgttta gaagaagagg aagaatggct tcttcattgg caacagctgg atattgctcc
    19021 aagtatgtat gcaagtaaga cgaccatttt tgagatgggg actgtgagtg cagcaaattt
    19081 tgaaagtttc aaggaagaac taagtaagct tgatgacgtt tatatggaag aaatagatta
    19141 tacgccaaaa caagtttatc tttcgtatgt tgctttaaaa aaatcagttg ctgctataga
    19201 agaaattgct gggaaatacg ggttcaataa agaaacgtat ccttatgaac aaaatcctaa
    19261 agaaaaattg caagaaacca aacagcaatt atccattgtt cacgagcagc aaaaaaagat
    19321 aacgacagcg ttaggtcagt gcagcggcta tatccaagaa tttgaatggg tagaagaagt
    19381 cacacttgcc gttgcagaac gggaaaaagt aaaagaacgg tttatccatg ccacttatct
    19441 gatcgttttg caaggttggg tagatgcaga agaaaaacag aatttgatgc atgtgttaaa
    19501 cgaaactgta tcagaagaag atatatacgt tagtttcgaa acaccaaact cagaagagat
    19561 tcagcaggaa gtccctacta aattgaaaaa ccatccgctt atcgaacctt ttgaattgct
    19621 gactgaaatg tacagtctgc caaaatatga agaagtagat ccaacgcctt ggatgacacc
    19681 attttatctt gtcttcttcg ggatgatggt cgcagatgtt ggttacggat tgctgatgct
    19741 cattgggaca atactagctc aaaaactatt tgttttaccc cgcgggatga aacgtttcgt
    19801 aaaattcttt gagattctct cggtaccatc gatcatctgg ggattgatct acagttcatt
    19861 tttcggacag gcattaccaa aagaactatt tggtatccgc ttgcctttcc caatcctatc
    19921 tacgacggat gacgtcaata cgattttgat tttatccgtg attttcggtc tgatacagat
    19981 cttggttgga ttattcgtgg cagcaaagga aaatatcagg aggaaagatt acttgagtgc
    20041 tgtcagtgat ggttttgcct ggcaagggat cctgattggg atcgttattg cattgattgg
    20101 tgtcatgttg ataaaaaata cagcatttgt ttatcttggc gcggggctat cgatcattgc
    20161 tgctttatgt atcttaatcg tgccgattat ccaatcgccg tctaaagtca agggtgctgc
    20221 caaaggagcg tataatcttt acggattgac tggttatatc ggtgatttag tcagttatac
    20281 acgtttgatg gcactaggta tttcaggcgg aagtattggc gcggcattca atatgctagt
    20341 tgctttcatg ccgccagccg ctcgattcag tgtagggatc cttttgatca tcgctttaca
    20401 cgcactgaat atgtttttga cactgttaag tgcctatgtc catggtgcac gattgcagta
    20461 tgtcgaattt tttggaaaat tttatacagg aggcggacgt gcctttgacc cgctgaaaac
    20521 ggcggaaaaa tacgtcaaca tcaatcataa gaaaaaaaac aataaataat tggaggattt
    20581 ttatacaatg atggattact taattagtca aaatggtgga atggtatttg cagtattagc
    20641 gatggcaaca gcgacgattt tttcagggat tggttcagct aaaggtgttg gaatgacagg
    20701 tgaggcagca gcagcgttaa caacaagtca acccgaaaaa ttcggacagg ctttgattct
    20761 gcaattgctt ccaggtacgc aagggttgta tggattcgtt atcgcctttt tgatttttat
    20821 caacttagga aatgatatgt cagttgtaca aggattgaac tttctaggcg cttctctgcc
    20881 gattgctttt acaggtttgt tttcaggtat cgcccaaggg aaagttgcag cagcaggtat
    20941 ccaaatttta gcgaaaaaac ctgaacatgc tacaaaagga atcatttttg ctgcgatggt
    21001 tgaaacatat gcgattttag gtttcgttat ttccttccta ttagtattga atgcctaagc
    21061 ataaaggagg aaaagcagat gaacgccatt gaaaaaatca tctctcagat gaatgaagca
    21121 gcagagcaag aaagagcggc gttagagcaa gaagagcgaa tgaagatcga tcaaaacttt
    21181 gaacaaaaac gtacgcaaat cgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa
    21241 ttacttgaaa aaaaataccg tcagcttagg aatcgccaac aagtagaagt acgacaagaa
    21301 aatttgaatg cgaagcagga atttttacga cgattgtttg cagatgcaat agctgaaatg
    21361 gagaattggg atgaatctga acaaatccag tttatcaaaa attcattgta ttcattacct
    21421 ttgacaggca aagtaacatt cattgctgga gaaaaatcag cagcttatct ttctcagaca
    21481 ttgctggatg aatggaacaa tgaacttccc tttatgatgg ttttaagtga tgaaactgtt
    21541 gctgatcaag cgggttttct gataaacgat caaggagttc agtataattt tcttttcagc
    21601 agccttgtac aagatatcca aggaacgatg agttttgaga ttgccaatca attattcgaa
    21661 taaggaggga tcagtgtatg gaataccacg aactaaatcc cttgattcgc ggaagagagc
    21721 ttgaactttt gtccaatgag acatttgaac ggatgattca aacaaattct gtcgaagcac
    21781 ttggtgaaat attgaaatcc acgatttaca gtccatatat ttatgacgga tatgaatatg
    21841 acttcgaaca gaatctttta aaggagcaga gccgtctatt tcgctggtta aaggaatcag
    21901 caccggagcc tgaaatcgtt tggatctata cgatgcgcta tactttccat aatctgaagg
    21961 ttttgacaaa agccgagatg acaaatcaaa atctggacca tttgtatatc gacgatggat
    22021 tttattcatt agaaaccttg aaggatgcca ttcatacgca agtttcaaat gaactccctg
    22081 aaagcattat ggacagtata cgagaagtac gtgattattt tgaagagtct accattcttc
    22141 agggaattga cgtgatttat gatcgacagt ttttgacaga gcagcgacgg cttgccgata
    22201 agctaggata tcaagaattg ctgacagaag tcattgcttt tattgacttg accaatatca
    22261 caacgatggc tcgaggaata ttgcagaaaa gaacatatgg gttcatgacg acggtacttt
    22321 ccagctcggg aagtatcgaa aaagaagtat ttttgaactt tgtagataaa gatatggaag
    22381 attacacgaa ttttctatta gagacagatt atgctgattt tctgcgccct gtcatccgtg
    22441 agggaaatct tgatctcgta aagcttgaac agttgaaaga tgattatcta agttcattgt
    22501 atcaagaagc acaaacccag gctttcggac cactgccact actagccttt ttgaacgcaa
    22561 aagaggtaga aagcaaaaac cttcgtttac tggttatcgg caaaagaagc catttcgaac
    22621 cagaaacaat cagaaagcgg gtgagacaag tctatgacgc ataagatcgg tgtagttggc
    22681 gacaaagatt ctgttttgcc attcaaattg tttggctttg atgttcgtta tggtacaacc
    22741 aagcaaaccg ttcgtcaggc aattgaagaa atggcaaaga atgagtatgg cgtgatttat
    22801 gtcacagagc aatgtgcagc attgagccca gaaacgatcg aacgttacaa ggaacaaatg
    22861 atacctgcta tcgtattgat tcccaatcat caaggatcat tagggatagg gctggaagaa
    22921 attcaaaaaa acgtagaaaa agcagtaggt caaaatatct tatagtatga aaaggagata
    22981 gaaattttgc aaattggaaa aatcataaaa gtctccggac ctcttgtaat ggctgagaac
    23041 atgtctgatg ccagtatcca agacatgtgt ctagtgggag atctaggagt tatcggagaa
    23101 attatcgaga tgcgtggcga tgtggcgtct attcaagtat atgaagagac ttctggaatt
    23161 ggtccaggcg aaccggttcg ctctactggt gaagctttat ctgttgaatt aggaccaggg
    23221 atcatttcac aaatgtttga cggaatccaa cggccattag atacctttat ggaaatcaca
    23281 caaagtaatt tcttagggcg cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg
    23341 tggttcgaac caacagtaga agccggagaa acggtgagtg ctggagatgt tatcgggatc
    23401 gtagaagaaa caaaagttat aaaacataaa atcatggtgc caaatgggat aaaaggaacg
    23461 atcaaaacaa tcgaaaaagg ttcattcact attgatgaaa cagtatgtgt cattgaaacc
    23521 gaaaatggtg acaaaaattt gacgatgctt caaaaatggc cagtaagacg tagtcggcca
    23581 atcaaagaaa aattaaaccc ggatgcaccc atgatcactg gacagcgagt gattgataca
    23641 ttcttcccag tgacaaaagg aggagcagca gctgtacctg gtccttttgg tgcagggaaa
    23701 acagtcgttc agcatcaaat tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga
    23761 tgtggcgagc gtggaaatga gatgacggat gttttaaatg aattccctga attgattgac
    23821 cctaatacag gagaatcgtt gatggaacgt acggtattga ttgctaatac atcaaacatg
    23881 ccagtagcag ctcgagaagc ttctatctat acgggaatca caattgcgga atatttccgt
    23941 gacatgggtt atgacgttgc tatcatggca gactccactt cacgatgggc agaagcactt
    24001 cgtgagatga gcggacgttt ggaagaaatg cctggtgatg aaggttatcc agcttatctt
    24061 ggttcacggc ttgcggaata ctatgaacga tctggaagag tcattgcatt aggatcagaa
    24121 ggtcgcgaag gaagtataac cgcaatcagt gctgtatccc cgtctggagg ggatatttcg
    24181 gaaccagtca cacaaaatac attgcgggtc gtaaaagtat tctggggtct tgattccagt
    24241 ttagcccaaa aaagacattt tccttcgatc gattggatac aaagctattc tctttattca
    24301 acagaagtag ggaagtatat ggatcatatc cttcaagctg actgggcatc catggtcacc
    24361 gagggcatgc gtattttgca ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg
    24421 attgattccc tttcagataa tgaccgtctg acattagaag tggcaaaatc cattcgtgaa
    24481 gattatttgc agcaaaatgc ttttgatgat gtcgatacat tcacttcaag agagaaacaa
    24541 ttcaaaatgt tgaagatgat cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta
    24601 ggtgcttatt taagtgagat catggacggt accgtatcga ttcgtgaaag gatcggacga
    24661 agcaaatata tcccggaaac agaattagaa aaactagatc aaattattgg tgaaatcaag
    24721 caaacgatgc aaacgatcgt ttcagaagga ggcatgagca atgattaaag aatatcgtac
    24781 gatcggtgaa gtcgtcggtc cattgatggc ggttgaaaaa gttgctggtg tcaaatatga
    24841 agaattaatc gaagtccgta tgcaaaatgg cgagattcgt cgtggacaag tattggaagt
    24901 ccaagaagat aaagcaatgg tccaaatatt cgaaggaaca agtggtatca atttacgtga
    24961 ctcttctgta cgatttttag gccatccttt agaactaggt gtatcggaag acatgatcgg
    25021 tcgagtattc gatggtttgg gacgaccgaa agataacgga ccagagatcc tgcctgaaaa
    25081 gcatttggat attaacggag aagtaatcaa tccagtagct cgggattatc cagatgagtt
    25141 tatccaaaca ggtatctctg caatcgatca tttgaataca ttagttcgtg ggcaaaaatt
    25201 accagtattt tctggctctg gtcttcctca taaagaatta gctgctcaga tcgcgcgaca
    25261 agcaacggta ctcgattcat cagatgattt cgccgtagtc tttgctgcta ttggaatcac
    25321 ttttgaagaa gctgaatttt tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc
    25381 tgtcatgttt atgaatttag caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat
    25441 ggctttgact gctgcagaat atcttgctta tgaaaaggga atgcatgttt tggttatcat
    25501 gacagacatg accaactatg cagaggcatt gcgagagatt tctgcagcac ggcgagaagt
    25561 tccaggaaga agaggatatc ctgggtatct ttacacgaac ttagctactt tattcgaacg
    25621 agcaggaaga atccgcggac taaaaggatc ggttactcaa attccgattc taacaatgcc
    25681 agaagacgat aaaactcatc caatccctga cttgactggt tatattacag aaggacagat
    25741 catcttgact agagagcttt acaagagtgg gattcagcca ccaattgatg tactcccgtc
    25801 tctttcgcgg ttaaaagata agggaactgg acccggaaag acaagagaag accatgcagc
    25861 tacgatgaat cagctttttg cagcctatgc acaaggaaaa caggcaaaag aattagccgt
    25921 agttttaggt gaatctgctt tatctgatgt agacaagatc tatgcgaagt ttgctgagcg
    25981 gtttgaaaaa gaatatgtca atcaaggatt ctacacgaac cgttcgatta ctgaaacact
    26041 tgatctaggg tgggaactgc tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga
    26101 cgacatgctg gacaaatatc tgccagaagg gaagtgagtt ctgatggcac gattgaatgt
    26161 gaatccgacc cgcatggaat tgactcgatt gaaaaaacag ttgacaactg ccacaagagg
    26221 gcataaatta ttaaaagata agcaagatga actgatgcgt caattcatcc tgttgattcg
    26281 aaagaacaac cagctgcgtc aagaaatgga agagtctatg acagaagcaa tgtctgattt
    26341 tgtattggca aatgcttcga tcacggaacc tttcatcgaa gaattatttg ttttgccggc
    26401 ggaaaacgtt gatttgtcta tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat
    26461 gaattttgaa tatgatgaac agctgacaga cacacctttg gaatacgggt atttgaattc
    26521 cagtgccgaa ctggatcggt caatcgatcg gtttactacc ctgcttccaa aacttttgga
    26581 actgacagaa atcgaaaaaa cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg
    26641 gcgagtcaat gcattagaat atatgacgat ccctcaatta gaagaaacga tctattatat
    26701 tcgtatgaag ctggaagaaa acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa
    26761 tatgggtacc actgactaag tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa
    26821 agcaaacaaa taaagaaacg tttgccggct gttcagcttc tcgcattagg attttttctc
    26881 ctgattttag ttggcggcgg cctacttacc ctaccatttt ttagtaatag tgggcatggg
    26941 acaaatttta tcgatgcgct tttcactgca acttctgctg tctgtgtaac gggattgaca
    27001 acattgaata cagcagagca ttggaacagt gctggtcaat ttttgatcat gcttttgatt
    27061 gagatcggcg gtcttggttt tatgatggtt ccgattctat gctttgcatt gataaagaag
    27121 aaagtcagtc tcagcacacg tattatatta aaagaagcgc tgaatctaga agaagtatca
    27181 ggtgtgatga agctcatgct ttacatccta aaattggcct ttgttatcca attgattggt
    27241 acggttgctt tatcttttgt atttgtgcct gaatttggct ggttgaaagg tatctggtac
    27301 agtttgttcc atgcaatctc tagtttttgt aatgcagggt ttgatttgtt aggagacagt
    27361 ttagccaatc accagcaaaa tgtttatttg atcatggtgg tatctgcttt gatcatttct
    27421 ggtgggttag gcttcatcgt atggcgtgat ttactggaat atcatcagaa aaagaagatg
    27481 acgattcatt caaaaatcgc attaaccgtt acaggtaccc ttttattagg cggattcgtt
    27541 gtgttctttt tcacagagcg caatggggca acgctagtgg aagggaatat gtttcaacga
    27601 ttggcaaaca ccttttttat gagtgtgaca cctagaacgg ctggatatta ttcagttgat
    27661 tacctccata tgagccatgc aggtctgatt ttaacgatgt ttttgatgta catcggcgga
    27721 acatctgggt cgacggccgg cggattgaaa acaactacgt taggtgtgtt gctgatccag
    27781 atgcgttcga tgttgaaagg acgtacgaga gcggaagcat ttggccgtac catccgacaa
    27841 ggcgcagtgt tgcgggcact tactttattt tttgttacgt tgacgttgtg tgtagtcgcc
    27901 atcatgattt tatcggtgac tgaaacgata cctaaaactt ctggcatcga atatattgct
    27961 tttgaggtct tctctgcgtt tggtacagta ggtcttacga tgggattgac gccagacttg
    28021 actgcaatag ggaaattgat cattatagcg cttatgtaca ttggacgagt tggtgtattg
    28081 actgtggtct tctctctgat gacaaaagca caaagaacac aatcaaaata caaatatcca
    28141 gaagaaagtg tcatgattgg ataatttcat cttctgacag aaacctagtg atttagtaaa
    28201 ataagaaaat aaaataattt ttcttgcttc ttggatactt taagtgcaaa atgttcgttt
    28261 attttttgag gcactacccg ttgttgaacc tcgggtagtg cctctttttt attgaaagga
    28321 atcaataaga tttgattcct ttatttatca ataggttata attcatattt ttttattttt
    28381 tcttttacga tacttagtcc agaagctaaa atttcaaggt tattagagga tagggaaatt
    28441 cgaacgaatg catctggttg cgcacgtgta gtaaagcgtc tggaatgata aagtcggata
    28501 ccgttagcaa gaaagtcatt ctcaattatc ttagggtcaa tctcaggatc aactggaaga
    28561 attcgatagt aagggacacg attcgttggt ttttctactt tgaaaagaga atcaaaaagc
    28621 tgattggctc tttttgtaag acttaattta gtttctaaga tcctatttgc ttcatttgaa
    28681 tggaggattt gagtgatgac ttccacatct aatccagaag ttttcacatt tatattaaaa
    28741 attgcttgtt ctatcttgct ttggacatgt actggaacta caagataagc aactcgcaat
    28801 ccagaacaaa taaattttgt cattccagct aagtagattg tttggttagg taataaattt
    28861 tggaaaggag gaatgatcgt atgttgatag taagttgtta aaaatgaatg gatatcatct
    28921 tcaataaccc atagaccttc tttttcaatc acttcggcta attgctctcg tcttttgtga
    28981 gagatttgaa accctatagg attattacaa gaaggcataa gaaagacacc atgaatcttc
    29041 ttttttctac attcctgaat caatatatca ggtcgcattc cttgctcgtc aaaatcaatt
    29101 ggaacgatct ctaaatgata taactgagcc aattcaatga aattagcgta tgtatatcgg
    29161 tctgttgcta ttctatcacc tggagagaaa agtgcagaaa gtgtgatagc caatccattt
    29221 tgaacaccgg aaacaataga tagagattct aaggacggaa tgactccttg agtttttaac
    29281 caatttgctg cagtagtcaa ttgaaaattt gtgcctttgg gatcttggta attaagcaaa
    29341 ccaacaagat ttgggttatt actagttgat tgaataaacg ggacaacgaa tttattacat
    29401 tcttcaaaag aactaaccag accaaaatca atgatatgtt cggttacttc attggtagag
    29461 atggtgatgg actcaaagac attgggagaa ataaatgtgc cacttccaat attcgtatac
    29521 agcaaacctt tctcaattcc atatttataa gccttgccaa cggtagtaaa atttaaatct
    29581 aaataatcag ctagctctct ttgggagggt aactgagtgt ttttctgtaa tttattcttt
    29641 aaaatatctt gttctaattg ttgaatcaac gatcgataat aaggacgctt caatttttgt
    29701 ttgtctggtc gccaagttaa cgggtaattg tcataagaat taataggcat tttctccact
    29761 ccttaaaatt gtataccata caattttacc attgattgta ttgattttag aagttaatct
    29821 tttgtgagga gggaattgtc tatgaaaatt attaattttg aggaagtagt agcttctttt
    29881 agttttgaag aagcgattca agtaatgaaa aaatgttttt ttgattatga aagtggtaaa
    29941 atcagccaaa atcctcgatt ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt
    30001 gcgatgatgc ctgcttattt gggggagaac agatattttg gcacaaaagt cattaccgct
    30061 tttccggata atcatcagta tcatttacct tctcatttag gagagatttt cttgtttgat
    30121 gctcaaaacg gactacccaa agcattgata aacgcaaatg cagtcacctg gattcgaaca
    30181 gctgctgtat ctgcgctggc aacagattac ttagcaaatc cgcatgcaga aacacttgct
    30241 ttaattggag ctggtcaaca agcattgtcg catttgactg cgatttctac cattcgtccc
    30301 atcactacag tgtttgttta cgatatttta gaagaaagac gaaatacttt tatcgctaaa
    30361 gctaaaaaac agtatcctaa aataacgttt ataaatgcaa tatcagtaga agatgcagta
    30421 cgtccagcgg aaatcatttg tacgttgacc tctagtaaag aggcatttct tcaaaaagag
    30481 tgggtaaggg aagacgcaca tatcaatgct gtgggcacat ttactcctga tacaagagag
    30541 ataagtacat cgttgatgga aacttctcaa atctatgtag atgattactc agctgcctta
    30601 acagaaagcg gggacctgct tatagccatg gcagaaggag atttagagga atcttgtatt
    30661 gttggtacat taggcgaatt agtttctgag aaaaaaacat tttataaagg agaatcgagg
    30721 ccgacgattt tcgacgcagt tggtctagct attgaagatc tttgttgtgc agaatatatt
    30781 tataacaaat tagagaaagg ggctttaaaa tgaacttaga ttattacgta gttgatgcat
    30841 ttgcagatga ggtgttcaaa ggaaatccag cagcggttta tgtgctagaa gaatggctgc
    30901 cagaaggtac gatgcaaaaa attgccattg aaaacaattt atctgaaaca gcatttacag
    30961 tgaagaaaaa ccaagaattt gagcttagat ggtttacgcc agatcgagaa atcgatcttt
    31021 gtggtcacgc gacattagca acagcatttg ttttatttaa ctattacaaa atacctgatg
    31081 aaacgattaa attttctact caaagcggta atctatttgt gacaagaatt aaagattatt
    31141 attacatgga ttttcctagt attatgccga aaaaagtgcc gatattgtct gaatatgaag
    31201 aagcgattgg ggctaagatt aaagaagcct acttagcacg tgatttattt ttcgtattag
    31261 aagatgaaaa aacggtggct aaattgcagc cagattttac agctatcaaa aattttgaat
    31321 taggaatagg agtcatcgtg acagcagaaa gtgtgcaaaa agactttgtt tctagaacct
    31381 tctttccaaa attaacaatc aatgaagatc cagtatgtgg atcagcacat tctaatctta
    31441 ttccttattg ggcagaaaga atgaataaaa caaaattgtc agcttatcaa atgtcatcaa
    31501 ggggcgggga tctagaatgt gaattaaaag aagatcgtgt gataatagga ggaaaagcag
    31561 ttttgttttc taaaggtacc tcgtttattt gatttctttc aaatgaatta aaaataaatt
    31621 ggtggagagt ggcaacctta tagctacaat ggttgttaaa aaaagacgca aactttttca
    31681 atttaatgaa aagtttgcgt ctcattttaa ttattggtgt ttgtttttta ttcaatcaag
    31741 agtaaaatgc cccatactaa ctatcctcgg atgacttcga ttttataacc atcagggtca
    31801 atgacaaagt agtaagaagg cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga
    31861 ccggcagctt tatgttgctc gtgtaatttt tctagatcat cagctgctat agcgatatga
    31921 ccatagccgt ctcctagatc atacgcacca tgatcatagt tgtaagtcaa ctctaattca
    31981 tagtcatccc caggaagtgt caaataaaca agtgtaaatt tgttttctgg aaaatcacgg
    32041 cggcggcttt cttcaaaacc aaatgccttt tgataaaatt cgattgatgc ttcaagatct
    32101 ttcactcgaa cacatgtgtg agccattttc atagattcat ctctcctttt ccttttactc
    32161 aatcataaca aaaagaagta aggaaatagt tgatttgtgc ttgttcatga gaaatttctc
    32221 atgtaaaatg gatagaaaat aaaactgtta aggtggaaac tattcatgga taaaccaatt
    32281 tttggtgaaa aaaagcaatt tgctgattat caaacccgtt atgcagcata tattgtcata
    32341 gaaagagatc agcagattgc agtaatcgag gctccaaatg gtgctttttt tcttcctggc
    32401 ggggaaattg aaggagaaga gacgaaagaa gaagcgattg tccgtgagct gatagaagaa
    32461 atgggaattg tggcagaaat ttcttcttat ctgggagaag ctgatgagta tttttactcg
    32521 aattatcgtg caacttatta ttataatcca ggatatttct ttgttgctga ttcttggaag
    32581 cgtataggag aaccaactga aaagaccaat aaaatttggt gggtgagccc tcaagaagcg
    32641 cttgtgaaat tgaaacgtgg tagccaccga tgggcagtcg agagatggat cgaaaaaaat
    32701 aaaaactgaa agaaagcgag tagatcagct ttctctcagt ttttttattt attacagtac
    32761 atattttttg attgcttcag ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc
    32821 tgcttctttg acaagagaaa cggcatttcc cattgctact cctagaccag catattcaat
    32881 catggataag tcgttctcgt tatctccgat agccatgatt tcatcttgat caataccaag
    32941 gtgctgtgcg agattcgcaa ctgccgcacc tttacttgct tctttattta gtacttcgaa
    33001 gtaatagggc gcacttttga ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc
    33061 aattgctgca tctaaaattt ctggttcgtc gatcatcatc attttgacga tgttcatgtc
    33121 tggtgtcatt tcttcaggtg ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg
    33181 gatcgtataa gcgctgatat ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg
    33241 gagatgactg ccgacacggc gtgccatcac ttcgatctct aggaaatctt catgtgtcag
    33301 tccgtgacgg gcgatgattt tacctgattt cgtttgttgt acgagtgccc cgttataagt
    33361 aataacgtaa tcattatctt gaaaaaggtt caactcgttt aactgttcct ggacacctgg
    33421 taagggacgg ccagtgcata aaacgatcga aacacctttt gcaacagcag cggcaatcgc
    33481 ttcttttact tcttttgtta cttctctttt ctcatttaat aaagtaccat cgatgtcgat
    33541 agcgactagt ttgatagaca taatttcctc caatatattt taatttattt caatcaatgc
    33601 gccattacgg atatggctgg caaattcttg ataagtttcg tcaaataaat cataatgatc
    33661 acgcaaggac ggatcaacca tttcttttgg aaagaaaaat cgctcatctc cttgttcttg
    33721 tcccgctaat gcagcaacaa gctgactaac ctgtgataat tctatcaaag ttccgtcatt
    33781 tcgcaaaatc tcgatttgtg tgcgatgtct gccttgttct gggcgataaa aatcataagg
    33841 tagatcgtag ctagaattca cagcagtgta ataaacagga ttatatccga ccttttctac
    33901 taacaaagtg agttcttgga ctaattttga atcccggttg ccagaaaaag tagcagattt
    33961 cagtggttta cgattcaaaa aacgtttagc taaatcattt aaaataggat cagcagattc
    34021 agaccattga gtaaagtaag tgcccaagac cccatcatca agtttgaggt attcttctaa
    34081 agtaaagctt ccgtcaagaa atggcagaag taattgagaa tggagttcaa aactgctggc
    34141 atcttcttga tacaactcgt gtgcgcgatg aagcagatgc tccaaaatta cttccatacc
    34201 acgggaaaca ggatggaagt acacttgcac atacatttga tatcggctaa cgatataatc
    34261 ttccactgca tgcatgccac tcatggaaaa agcgatcccg ccttcataag gacgaatcac
    34321 acgtaatatt cgggtaagat caaatgttcc atattctgtt ccagtgaaat aagcatcccg
    34381 aagcaaatag tccattcgat ctgcatcgat ctgactagaa atcatttgga caacttgtgg
    34441 gttggggtaa gttttttgga tcacactagc taccttttca ggaaaacctt cttctactcg
    34501 gttaagaatc tgatacactt ctgtttctgg agaagtgatg atctctacag tgatggcttc
    34561 atgattcgta tggaagatat gttcaaatgt atgagagtaa ggaccatgac cgacatcatg
    34621 caacagtgcc gcacataaag tcaccagccg ttcctgatca tcccagcctc cgttgcctat
    34681 tttttctttt gaaaaatttc gagaaaagat atcgcagatt cgtcttgaaa tctcataaac
    34741 gcctaatgaa tgagtaaaac ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc
    34801 taattgttta atgcgtcgta gacgttggac ttctttcgaa ttgatcaaat ctaagattac
    34861 ttgatgctgg acatggatat agttatggac tggatcacga aaaacttttt ccataggcag
    34921 catttgatat ttataagggg ttgtcataat gtagctcctt tatgttatta cgttgttcca
    34981 ttctctggat ttggttgttc cattgctcca gctggatttc tttttttagc cagtcggtag
    35041 aatcctgtaa atcacttgca gaatacagct tttcaaaagc ttttgcaaat gcttctttga
    35101 cttcttttac aaaaaggggt tggtcaagca gttcttcaag tgttgccatt gtagcaggat
    35161 ccactgcagg atagccattt gtaccaaagt cttcttttaa agcagattga taaaatcgct
    35221 tcatgacttg cccgcgtttt tcctgattac cgttgacact taagtacatc ataacagaaa
    35281 tcccattttt gatccgacgc tgagcgatac cggcaaattt tttgccatgg atgcttaaat
    35341 cataagttcc aggacaataa gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa
    35401 aagcgtattt agtcaatagg agcatttgct cgtaagcgtc atctatggtt attttttttg
    35461 tgtcactttg ggggaaaatc aaagaaatat tcaaaattcc ttcatctgat acgatcccta
    35521 atccacctga atttctgatc actggagcgt aacctgcatc tttcagtgtt tggatcccgt
    35581 cacttagaaa aggagttcga acatccttca tacctaagat gactgtgcag tcatattgcc
    35641 agaaatgtaa aaagatttgc tggtgttctt tagaatattc cgttaatata tctgttaatg
    35701 caaaagggga aaagtcgttt ttttgataaa tgtgctgatc tagtcgaatc gcagggttca
    35761 ttcaaccggc tcctttgtat aaattcatat tttctacaat attatactct aaaatcaaag
    35821 agttgttttg gaaaaaaggt tttctgtaaa aatggacaag ttggttgaca aaacaaaaga
    35881 aactaggaac aatgtagcta tgagaagtaa aggagaatga ataatggaat tatcacaagg
    35941 aacaccgatt tcaatcaaat tgcgtacaaa agtaaaacag caaggggaag tgcaagattt
    36001 ttattttgat ttgaaagggc aaatggtgac aattggcgat acgctgtaca tacgttataa
    36061 agagattcag gaggagacgg gcgatgagat tcctgtaacg atcaaattag ttccagatgg
    36121 tcatgtacag ctgatccgtg caggagaaat gcggatgcgt ttgaaatttg gctataaaga
    36181 acggttagaa acatcctatc gaacaccata cggtatgatc cagattgcta cttttacgaa
    36241 agaactgcat gttagtttga aagaccgtcc cacaggaggg aaggttcgaa tcgattatga
    36301 tctgttcatg ggaccggaaa aaatcggcga atattatttg accttggatt ttacagcgta
    36361 aatataaaag aaaagtttga ttttttgtgt ctgattttgt atgataagga gtagactgtg
    36421 aaaggacgtg tcctgattgg aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc
    36481 aatgatcgaa gtagcacacg cgatcttaga acaacgtggc gatgtcatgg atttttctga
    36541 tttagctaac cagatccaaa actatcttgg gaaatcagac agtgaaattc gtgattcact
    36601 cgcacaattt tatactgact tgaatattga cggcagcttt atctcattag gggataatcg
    36661 ttggggcttg cgttcatggt atgctatcga ttcgatcgat gaagaagtaa accatggaat
    36721 ggacgaagag gatgaagata ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat
    36781 caatgatgat gaagacgcga tcgattacaa tgacgatgac ccagaagata cagatctgac
    36841 agaagaagat gacgatgatc tgtttgatga cgatgatgat gaagatgaag aaatcgctgc
    36901 ttataattct gacttgcaag aaatcggagc agatgacccg acagatgatg aagaagactt
    36961 gccaggaaat atcgaagaag atttaagtat catagacgaa gacgatgatg atgaagagga
    37021 agaattttct gactctgaaa aagaataaac attattttct tacgagaggt tgtgacagag
    37081 acgatcagcc tatcagaata aggaggtgtg acacgatttt tgtcacacct tcttttttgt
    37141 atataaaaat cccaagtaaa tacataaaaa ataaaaaaat tgaaactttt agcgcacctt
    37201 gtacgtcata ttaagtgagg cagaaaggag tggagaatga tttttactgg atataagaaa
    37261 aaacggcaga tcgaaaaata ccgtcacctg ctgaatggac tcatcgaaac tgattatcaa
    37321 aaaatgttcc gtatcacgat gaattacgta catgataaag aagaggcctt agatgtgatg
    37381 caagatagtt ttcataaagc cctgaactat tttgaaaaag aaaaagagat cgagcatttt
    37441 agtgcatggt tttatcggat attgatccgc acggcattag atagttggcg cagacaaaaa
    37501 aggacgcctg tggacttatt tgaaattcaa gatatccccc aacagttacc tttagaaact
    37561 tccgttgcag agctccatat gattttagat aaaatagaca gtccggaaaa agagattctg
    37621 atcctctact tttttgaagg atttcgtttg aaagaaattg cgactatctt ggatatgaat
    37681 gaaaacacag ttaaaacgaa aatgtaccgc tcattgaatg ctttacgtca aatcctagaa
    37741 tgaaggagag aagagtgcca tgtattccca aaaagaaaat ttaaaaaaac taaagaaaac
    37801 ttataaagaa caagcaattc ccaaagggct taaagaggag atccgccagc gttttattat
    37861 cgaagagcaa tcatttataa aagcaaagcg gagaaagaga cgtctccaag ggttcggagt
    37921 atctttggct atcgtaatga ttacaacaag tagccttctt tttaatagtc aagttcgaag
    37981 ctttgcagaa gatttaccca tcttaggatc agtcattgaa ttgatcttag gcgaacgctt
    38041 tactgatcga tctgaaaaaa tcgatattca ggtaccgaag atcaatacac aaaatgagaa
    38101 agaaaataaa acaattcatg gattaaatca aaaatacttt cgcgaaggac aagcagattt
    38161 tgaaaaagct gaaaaagaat atggaaattt tgaaacggat cattaccaag ttttgggaga
    38221 ttatcagaaa atcgtagacg ataaccggtt tctcgtgatt gaaaggcaga tcacccaaac
    38281 agcagcagat agccatgttg aaaaaagata cgacacaatc gacaagaaaa acagtgtcca
    38341 gctctcactt ccacttttat tcaaagatga cacctatcta tctgttttga caaaagaagt
    38401 caaacggcag atggccgaac aagtcaagga agatccatct aagtactatt ggactgagca
    38461 agatattcaa gaagggacga tagaaaaacc aacacttgtt acgcctacac gaagttttta
    38521 tttgaacaaa gagcatcaat tagttttgac tttttctcaa tacgaaattg ctcctggtta
    38581 tatgggtaca ccagaatttg tcattcctaa gtccgtgaca aaaacgattt tggcatcgga
    38641 agattatttg gatcattaga cgagaagtag ttgcgactag cagtttcaat tttcaagtaa
    38701 gtacaagtaa ataataagaa gagaaaaaga gaaagaagag atatagtgaa aagaaattat
    38761 ttagttatca gtattattgc tttatccgtg cttttgtttt tcgaaattac ttcggtggcc
    38821 tccttgatca ccgaagtaaa aggacaaaca cactcttcgg agcattcgtc acaatctttc
    38881 aaacaatcag aacaatcaac cattaaaagc caatcaactg aaacagctaa cgtcaaagaa
    38941 gccattcaag aatcagcgga aaaggtacaa agtcagtcat cacaatcaac acaagacagt
    39001 acaaaaacga gccgagatcc ggctgtcaaa agcgtcgcaa tcagctttga tgatggacca
    39061 ggagcgacca caacaccgca gcttctgcga atcttaaaag aaaaaaacgt gcatgccact
    39121 tttttcgtct taggagaaaa tacggcgcag catcctgaaa tcgtgaagca gacagctgaa
    39181 gcagggcacg agattggcaa tcacacatac gatcatcaga atctggccac tctttctgcc
    39241 cagtcgatga cggaggaagt gacgaaagca gacactgaga tcaaaaaagt cactggcaaa
    39301 acccccactt ttgttcatcc gccctatggt tctgtcacga atgtaggagc aacgatcatc
    39361 cagcgaccga tcatcgaatg gtctgttgat tcagaagatt ggaagacaag aaatcctgat
    39421 ttgattttgc agaagatcca agcaacagta tacgatgggg caattatttt attccatgat
    39481 atctatcctg aaacgattcg tgccgttcct caagtgatcg attatctgaa agagcaaggg
    39541 tatcgcatca cgacagttgg ggatttatta gggcatccaa cggctgtcga gaactattat
    39601 ggcagaaacg atcatcggcc agttcagtaa gtgagacaag tatctttaat aaataacaca
    39661 ttttaatacc cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt
    39721 aaatcaacaa aaaaacgcat atagtagtct caaaactccc aagaaatgag gactacatat
    39781 gcgttcatca aaatatactg aacattatac agatactttt ataacattca aggaaattat
    39841 gagaactgtt tctaacatat accacaattg tgtaccagat aagattaaaa accgaagaaa
    39901 tactgat
//
LOCUS       DACDLI010000016        39068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDLI010000016 DACDLI010000000
VERSION     DACDLI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.016"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..532)
                     /locus_tag="IV353_000929"
     CDS             complement(<1..532)
                     /locus_tag="IV353_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009200.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNH"
     gene            complement(643..1512)
                     /gene="mdcB"
                     /locus_tag="IV353_000930"
     CDS             complement(643..1512)
                     /gene="mdcB"
                     /locus_tag="IV353_000930"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9009201.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(1537..2613)
                     /locus_tag="IV353_000931"
     CDS             complement(1537..2613)
                     /locus_tag="IV353_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9009202.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(2637..3230)
                     /locus_tag="IV353_000932"
     CDS             complement(2637..3230)
                     /locus_tag="IV353_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9009203.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(3234..4910)
                     /gene="mdcD"
                     /locus_tag="IV353_000933"
     CDS             complement(3234..4910)
                     /gene="mdcD"
                     /locus_tag="IV353_000933"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9009204.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(4900..5199)
                     /locus_tag="IV353_000934"
     CDS             complement(4900..5199)
                     /locus_tag="IV353_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9009205.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(5301..6947)
                     /gene="mdcA"
                     /locus_tag="IV353_000935"
     CDS             complement(5301..6947)
                     /gene="mdcA"
                     /locus_tag="IV353_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9009206.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(6954..7865)
                     /locus_tag="IV353_000936"
     CDS             complement(6954..7865)
                     /locus_tag="IV353_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9009207.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            7985..8842
                     /locus_tag="IV353_000937"
     CDS             7985..8842
                     /locus_tag="IV353_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9009208.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(9029..9391)
                     /locus_tag="IV353_000938"
     CDS             complement(9029..9391)
                     /locus_tag="IV353_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009209.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            9737..11176
                     /locus_tag="IV353_000939"
     CDS             9737..11176
                     /locus_tag="IV353_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9009210.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTASILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(11183..13735)
                     /locus_tag="IV353_000940"
     CDS             complement(11183..13735)
                     /locus_tag="IV353_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9009211.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(13849..15180)
                     /locus_tag="IV353_000941"
     CDS             complement(13849..15180)
                     /locus_tag="IV353_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9009212.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(15146..16624)
                     /locus_tag="IV353_000942"
     CDS             complement(15146..16624)
                     /locus_tag="IV353_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9009213.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(16929..18764)
                     /gene="tdc"
                     /locus_tag="IV353_000943"
     CDS             complement(16929..18764)
                     /gene="tdc"
                     /locus_tag="IV353_000943"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9009214.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            19234..20661
                     /locus_tag="IV353_000944"
     CDS             19234..20661
                     /locus_tag="IV353_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9009215.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(20753..21118)
                     /locus_tag="IV353_000945"
     CDS             complement(20753..21118)
                     /locus_tag="IV353_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9009216.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(21115..21480)
                     /locus_tag="IV353_000946"
     CDS             complement(21115..21480)
                     /locus_tag="IV353_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9009217.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(21672..21920)
                     /locus_tag="IV353_000947"
     CDS             complement(21672..21920)
                     /locus_tag="IV353_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009218.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            21919..22047
                     /locus_tag="IV353_000948"
     CDS             21919..22047
                     /locus_tag="IV353_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9009219.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            22104..23798
                     /locus_tag="IV353_000949"
     CDS             22104..23798
                     /locus_tag="IV353_000949"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9009220.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            23898..24071
                     /locus_tag="IV353_000950"
     CDS             23898..24071
                     /locus_tag="IV353_000950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009221.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            24449..25582
                     /locus_tag="IV353_000951"
     CDS             24449..25582
                     /locus_tag="IV353_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9009222.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            25613..25933
                     /locus_tag="IV353_000952"
     CDS             25613..25933
                     /locus_tag="IV353_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009223.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(25962..26426)
                     /locus_tag="IV353_000953"
     CDS             complement(25962..26426)
                     /locus_tag="IV353_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9009224.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(26556..27809)
                     /locus_tag="IV353_000954"
     CDS             complement(26556..27809)
                     /locus_tag="IV353_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9009225.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(27838..28155)
                     /locus_tag="IV353_000955"
     CDS             complement(27838..28155)
                     /locus_tag="IV353_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9009226.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(28181..28501)
                     /locus_tag="IV353_000956"
     CDS             complement(28181..28501)
                     /locus_tag="IV353_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9009227.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(28966..30381)
                     /locus_tag="IV353_000957"
     CDS             complement(28966..30381)
                     /locus_tag="IV353_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9009228.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEERYMDEKGIVQDDYRIEF
                     IGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(30397..31713)
                     /locus_tag="IV353_000958"
     CDS             complement(30397..31713)
                     /locus_tag="IV353_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9009229.1"
                     /translation="MRGSGMSFMDKLAKVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISYLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(31863..32561)
                     /locus_tag="IV353_000959"
     CDS             complement(31863..32561)
                     /locus_tag="IV353_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9009230.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(32850..33843)
                     /locus_tag="IV353_000960"
                     /pseudo
     CDS             complement(32850..33843)
                     /locus_tag="IV353_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            complement(33924..34757)
                     /gene="msrA"
                     /locus_tag="IV353_000961"
     CDS             complement(33924..34757)
                     /gene="msrA"
                     /locus_tag="IV353_000961"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9009231.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(34991..35734)
                     /gene="truA"
                     /locus_tag="IV353_000962"
     CDS             complement(34991..35734)
                     /gene="truA"
                     /locus_tag="IV353_000962"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9009232.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(35925..36560)
                     /locus_tag="IV353_000963"
     CDS             complement(35925..36560)
                     /locus_tag="IV353_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9009233.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(36707..37948)
                     /gene="sstT"
                     /locus_tag="IV353_000964"
     CDS             complement(36707..37948)
                     /gene="sstT"
                     /locus_tag="IV353_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9009234.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(38269..38946)
                     /locus_tag="IV353_000965"
     CDS             complement(38269..38946)
                     /locus_tag="IV353_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9009235.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKNVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
BASE COUNT        11870 a         8172 c         6633 g        12393 t
ORIGIN      
        1 tatgatttct tacaccgtct gctttgtttt tttgcagaac tttctgatgt tcggcaaaca
       61 gcttgtctgt ataacagctt ctggcttttt caggagtttc ttcttcccaa gcggattgga
      121 tcttcatgaa tatcttttgg atttccagaa actgtgcttt cttttcttta gccgttcctg
      181 acatgcggga caatatctcc gctttcagcc ttttcttttg tcttttattc ttgacgatag
      241 aaaaagcaga aacccctcct actgacaaaa cagctaatgc aagaaatccc gtttgctcat
      301 aactagaagg acctgtacct ggaccataat acccataaga atctctgccg taagaacgac
      361 tgccaaaata actgccccca tatgaacgac caccaaatga acctctgccc gaacttcgac
      421 cgctagatgt acctccacca tgaccacccg cgacagcgaa tccttgggtc ggcagagcaa
      481 acaaacatag gcccactaac aaaatgattg ctaaataatt gatttttttc attgtcttcc
      541 tcgttatctt ttattacaaa aaactatttt tatgatagcc ttgctaaaag gaagagtcaa
      601 taaccagatg cataaactgt ttttttagca attttgtctt atttagtcat tgagtaacat
      661 cggacaaatg tatgtgtcgg cagtccataa ttcctctatc ttcagcatga acaagcttgc
      721 agacaagaga tctgcgcttc ctccaggaga gatttggttt tcctgacaaa agtgcaacag
      781 tgctgtaaat ggacgatttg gtaaagcact ttgactagca gcatgagcaa gtttttgcat
      841 ttccactaac gttttctgat cactgcgatg caatacgcat gtgtcatcta gcgaactcat
      901 caaaactaac aacatatgca tttgtttctg tacatatgaa tcattatcta gctccttagc
      961 tgcttggatt gcaagcctga tatgcggata ccctaatatc gcttcttcat atgcaccatt
     1021 tacgccatat ttttgcttca ccctatgacc atgagtggtt tttttatgac gatattttgt
     1081 atcaggaaaa gcagcaagct tgctgacatc atcaagtatt ttcgataaag aaaaaggctg
     1141 ttccatactg attttttgtg caatgacact gatcaatagc cccattgccc aaatcgctcc
     1201 tttatgggta ttgatgccat ttgtagcaat cagcattgcc ttttccgctt ttcgcccaat
     1261 ctcagcaatt ctttctctta actcctgatc aatagcatga tgccatgcta tttgagccat
     1321 ttcttcaaaa aaaggagtta gcgtcttagc agacttaaga aataaagcat agtccatatc
     1381 ggaatgtgca cccgcatcca gtgcatccac taatccaggt tttggactta atgttacttc
     1441 tgcgatcaat gcttcctcta ccaacgcagc gagttgctga gtcactgctt tttttcgttc
     1501 gtcatttatc atgacactca ctccttcttc ctactattac ttgctgaaac cttttttgat
     1561 agctacccaa atgatatgaa gtaaccagct taaagcaaat gcacaacttg ccgtcattag
     1621 ataagctaca agaatcatga ctcctgaagg taactgcagc caactatctt taaacaataa
     1681 taacagtact tggaaaacaa atacattggc taaatacgta cgataagcat aaacagacaa
     1741 ccatttgaac aatggcaatt ttggcgaatc gtttacaatc attttggaag cgcctatgaa
     1801 gacagcaaag atgatgacta aactgtataa actttggatc gtattcaagt aaggtgcatt
     1861 cgcaaagctc aagtctcctg gatgtgcgag caatgaacgg acagaaaaga acgcgataac
     1921 taaggcaaca ggtaagaaaa gataacgtac tctgtccaaa tattgataga ctttttcgtg
     1981 ataaacaaat gcggctaccc cataaatcat gaaactagca acaaaccgat ctaataagta
     2041 ccagctgtca tgataaggcc cagtcaatac ttgtgtttga tagaacagat accatgcgac
     2101 atataaaatc gttgaaccaa tcaatactgg ccatacatat tttttattag caaatacttt
     2161 gtaaccgagc caaacaaaaa acggcatgaa caattggatc tgcagcatca taacggtata
     2221 ccataaatga ggcgctccat cacctgtgac atatttcagt aaaaagcttg cagtatccgt
     2281 atacggagtt tgttgttgga ctgctggaaa tagccaaaga taagccgtcg tccatagaag
     2341 atacggaacg actagttcaa aaaacttttc tttcaaaaat tcccaataag acaaatgctg
     2401 acttgttttt accatattat aaacaattgc aaaaataaac attggtgctg tgtatttaac
     2461 gatgacgtaa aaggtaccaa gaaaagctgt atttgttgcg ctgtggctgt ttgccaaagc
     2521 aaagctcaat atcgtttgca tcataacagc cgttacagct gatactttct ggaagtcact
     2581 tacgttgatt ttgatttttt tgttttcaaa catctctttc tctctctttt tctttattac
     2641 caaagttctt tggttaattt aggtccttcg actgttttca atagaatttt tttctctggc
     2701 tctctcaaat actcttttag tgaaaaaccg ccttgccgag tcactacttg ggtatcgaat
     2761 tgctcaaaaa aagcaggatg cgattgaatt acctgaattg gcaattcaat cggtgaattt
     2821 gcgtacagca gcaaatcaaa atcactggtt tcttttactg ccggaatccc agtcgataat
     2881 tcaaatccta agctgccgcc gattccccat ttacatcctg ataatatgtt tctagcagca
     2941 tgaaattgct gataaacagg ataatccatg attttctctt gtctatacga ttcttgttcg
     3001 atgatgtccc acggatgaat gacttgtttg acggcatgtt ttgagatttc tgttgaccat
     3061 ctctgattgc gggcaggtcc acgtaaacca atcggtagta tttctgcgtg atccgtcgac
     3121 gcacgacgaa ctgttccgaa catcttttca gttagaacga cccattcagg caaggtgaac
     3181 ccttctattt catgtttatc aaaaaggaca atatcatgtg cttgaatcat tttttatgca
     3241 tcccattctt cgtccatttt ggcacggact ttatttgttt cgacacgtcc gaatgttttg
     3301 gctgtttcgt ttgtgtatcg atttgtcagc atcgtgtcat ttgtattgcg gacatcttga
     3361 atggcttcat tgattgcttc gatgactttg ctgacatttt gttctgaagc agcttggtcg
     3421 gtgatatctt ctaggaaatg atagatcgct cctagttttg tataattatt gatatcgtaa
     3481 gcgatagctg gaactttttc agcagctgct tcgatttcag caaccgaacg ttttgtcaca
     3541 cgtgcagcac tttctttaga catcgcttgg acggtaattt ctttattgtt taaagcaatc
     3601 aagcggcttg attgtaatcc atgtgctaaa aatgcacctg aaatagcatt tcctgcaatc
     3661 aatccaataa ctggatgtcc agcttgacgg agttttgcat aagcagcagc actattagcc
     3721 aaagccacat ggataccgat caattcttcg ttatagccgt atgcttggct tggtacatcg
     3781 atcacaacga tgattggccg tttcactttt ttgtctttat cttcttcgta aacgtgatcc
     3841 aaaatttgtc ccatgcggaa accttcaact aaaccaactt caccgtttcg tacacggtac
     3901 atcgggtttt taggatcagg tacgattgat gtcacgatgg cttcttctat gaatttactt
     3961 gatttaccat aatacacagt actaatagaa gaagttggat ttttcatgcc agttagtgcc
     4021 gcaaaccaat cgtaaccaac acttgctgca gctggttcat ttccttctgc tacttctggt
     4081 aagtcaattg attttgcttg atggttagca attgcttcat tgtaatcgtc aatggccatt
     4141 ggttttgaaa gatctaaaga atcaagtaat gataaataaa atgctgagct ttcactgcga
     4201 tgcgtgtctt ttcgttcgtc cattgctgcg atgacagcag ctttgattgc ttctacacta
     4261 tcagtcacta attcatcaat gatttttgat tctacccgtt gacgagatcc taatgtattc
     4321 caaatcaagt ctttgtcact agaatcaaat tctcgaacgc ctgcttcttg ttcaataact
     4381 tctggtccat tcaaaccgac tctcgctttt tttgttgcga ttaaataact cattaatgct
     4441 gaagtaatcg acataccgcc aaatgatcct acacgtcctg gaatcaaacc gatgactggt
     4501 acatattgtt ttagagcaat gattacattt ccgatttctg aaatagataa cagtccgtag
     4561 tttgcttctt gcaaacgaac accacctgta tctagtacaa tgactggaaa gatctcattt
     4621 ccttttttat tttcttctaa cacatgttca agtgcagcaa cgatttttgc accggaaact
     4681 tcaccgatac cgccaccttg gaattttcct tccatagcga ttactactgc ttgtttgcca
     4741 ttgatcgtgc cacggctgac aacgatcccg tcatcacttt cagggacgat tccttgtgct
     4801 ggtaaacttg gcgcgatgat ttgatcgaat ggatcaatca attcacgtcc attgtcaagt
     4861 agagcgttca ctcgttctct ggcgtttaat tctacaaaac tatttttcat ctgaaagttc
     4921 ctccattgct tgcgtcaatc gtaggtacac aactcctggt gtagcaccga agtcattgat
     4981 cacgatatct gctgaaattg gataacgatc aaaaaatctc gttaatacat tgttccatac
     5041 ttcgccgaag ccatcgcttc ctgtacaaat ttttacagta gattctgttt tttctgttgg
     5101 aaacatcaag atttccaaat ctcctgaacc gaccactcct acatggattt tcttattgat
     5161 cggctgtttc gttggatatt tataagctaa ttgttccatg gatctctccc ctttttgtaa
     5221 aacagagcat acacgttctg cttattcgtc tttcttcttc catctgggta aatggcgaca
     5281 aaaggttttt ctcgccaatt ttatgaccaa gttctgaatt tcattggtgg ttcatacagg
     5341 ccatctgacc attcgatcaa atcatcgatg gtttgtgcag ctaataatga acgtttcgct
     5401 tcactacgtt tgatgcctag atcttctggt aatttcacga tgccggctgc acgcaagcta
     5461 tctagtgatt ctttcgtgct agtcattcca agaggtgtaa caccgccaat tgctgcaatt
     5521 gctgcttgtc gttcttcttt tgtttttgct ttgtacaaat aagcaattcc ttcttctgtc
     5581 acgatatgtg tagtatcttc gctataaatc atcactggtg cattggctaa gcctgcttgt
     5641 ttttgaacag ctagtgcatc taattcttcg acaaagactg gttttttatt tgaaccatat
     5701 gtttcaacca tttgaacaac taatttttgg cctttcccta atggatcatc attgtcgcga
     5761 agtgattgcc atgctggtgt agaatgacga cgaccgccag gattatgtcc catgttaggc
     5821 gcaccgccaa aaccagataa acgtcctttt gttacggtag aagagtttcc ttcgtaatct
     5881 aattgcaagg ttgaaccgat aaacatatcg atggcatatt gtcctgcagc ttgtgataat
     5941 gttcggttac tgcgcatcgt gccgtcttta ccgacaaaaa agacatctgg gcgagcagca
     6001 atatattttt ccataccaac ttcgccacca aaactatgga tgctttcaac ccaacctgat
     6061 tcgatggctg gaatcatcgt tggatgtggg ttcaataccc agttcttcgc gattttccct
     6121 tttaacccta gtgattcacc ataagttggc agtaacaatt cgatagctgc tgtattgaat
     6181 ccaataccgt gattcaaaga ttgtacgccg tgttctgcgt agattccttt gattgccatc
     6241 attgccatta gaatttgcaa ttctgtgatg ttttgaggat ctcgtgtgaa taaagcttct
     6301 aattgataag gtttatctgc tttcacgaca acgtctaccc agtcaccagg gatatctaca
     6361 cgtggtaaag aatcgacaac ttcgttgact tgaacgatca cgataccatc tttgaaagct
     6421 gcagcttcga tgattgttgg tgtttcttcc gtattgaaac ctgtgtacaa gttgccgtct
     6481 ttatctgctt tatctgcagc tactaaaaca acatttggaa tcaaatcgat gaataagcgt
     6541 ccataaagtt ccaaataagt atggatatca cctaatttca ttgtgccgtc ttcaatcatt
     6601 tgggcgacac gcaagctttg tgcaccagca taagagaaat cgatcttgct tgcgattcct
     6661 ttttcaaaaa tattcaaatg ttctggtcgt gagatactag acatgatcaa atgcagatca
     6721 ttcacttttt ctgcatctac tttttcaaga ctttctgaaa gaaaacttgc ttgtttttga
     6781 ttgtcgcctt ctaatacaac tttgtcgccg ggagcaatca gtttttccat tacttctacg
     6841 attttttcag tttctacata cttgccattg agcaagttgc tgattttttc caatcgctgc
     6901 gctttttttt cgcgtttaga agcccagttg cggtttggtt tttccataat tagtcatctc
     6961 catttttgat atagaaacgt ggcgtcatca ataccatgtt ggtctagatt gatcgtacgg
     7021 atccctttat ctacaccaaa ttttgtatga atcagctttg tcaaagtatt gccgggggga
     7081 aattcaatcg tcagatccat tcctaattct tttgccaagt ccatcatttg gttccactgc
     7141 acagggaaag ctaggttttc caacaaatct ttacgaatct cttctgcgtc ctgagtggct
     7201 cttccatccg tattttttat atacaaacat ctagcatcag atagatggac agactgcata
     7261 tacggcttga atttcttgac cgtatcattc atcaagagac agtgagatgg cacagggact
     7321 ttcagcagct tcgctgtttg cgcttggtgc cgtttggctt ctgccaacgt aacggataaa
     7381 ccttcgatac tcccagataa cgtatgttgc atcggacaat tttcatttga cagataaact
     7441 ggatggtcag gatcaaatgt ttttgctaca atttcctcag cttcgcttct cgtcacacct
     7501 actactacgc ccattgcata accgcttgga taagcttctt tcattaagac tgcgcggtga
     7561 tagactagtt ccgctgcatt ctcaaatctc aaagatcgac aagcaacagc tgcacaaaaa
     7621 gcaccaatcg aatgtcctgc tgcaatatca ggctgaatac cgtttttttc catttcatct
     7681 aaataaaata ctgcttttgt cagtaatgct agctggataa aaaccgtatc ttgatagttt
     7741 tcttctttat caactaaccg ataccccgtc acattttcta aacgttccat gtacatttca
     7801 gggatatccg ctaacatccc taacttctga ctgccttgtc caggaaataa gaaacctgta
     7861 ctcatgagcc tcgcctcctc agcacttact ttttccactg ctatcttagt caatttctgc
     7921 tataataaaa aatgccaaaa aaagaaaaaa ccataacaaa aaggtatgga tgggatggat
     7981 aattatgaaa ttgcttcatc tgcagtattt taaaaaagta ttcgaaacaa aaaatgttac
     8041 ccaagcagcc aaagaattat ttatttctca acctgccttg agccgtgcga tcaaacattt
     8101 ggaaaacgaa ttaggtgtcc ctttgtttta ccatagcggc agaaacattg agacgaccgt
     8161 ttatgcagaa gaattttatc catatgcagt caagacatta gacacattag aagaaggaat
     8221 caaattattg tcttcggtca atgagaagat cgttacttcc gtcatattgt atttagaggt
     8281 agcctctgtt tctattccga acttggtccg aatttttatg gaaaagcatc ctgatatcca
     8341 attaacgatc atgcaacatc atttaccaga tgatattacc gatcctgtac tgtacatcac
     8401 ttcggagatg aagcctggca tgaccaatat tcctatcatg aaagaacctg tttatgtcgc
     8461 cattcctaag aaacatcctc ttgctaaaaa agaacatctg caattagaag atatccaacg
     8521 cacccccatc ttgatgctgt caaagagcaa cgccttccgt catacactgg atgcagcact
     8581 tgaagcgaaa agtatcgaat tgacgatcag ttcgacgaca gatgatcctg cgacattacg
     8641 ttctattgtc aagcaaggat tagctgttag ctttttccca aaagtttctt ggtcttacga
     8701 taaaaatgat cctttcgtct tgcgggaaat caaagatttg cccttgacac gaacaattta
     8761 tttatcttct agcttcacag aagatcatcc tttgatcaaa atgattgcaa aaacgatgcg
     8821 ggaattttat ttaggaaaat aaaaaaaaaa gagctgggac aaaattcttg tcgcagctct
     8881 tttttcgaac aaacggtgtt ccagaagtaa tttcttagaa aataagccga aatttcccaa
     8941 aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctaaa catttctgtc
     9001 acaacctctt ctctattgtc ctagacattc attgcgttga tttaaaactt ccatcaatca
     9061 aatctgctag ttcatctagc ttttctattt tacgtagatc aatagagatc caatgttctt
     9121 tattcatgtg ataggctgga aaatacccct cttttcctcg caaaattccg ccaagttcag
     9181 gatcgacctt caaatcaaga atattgatga tcctatggtc acttgcatca tcatagattt
     9241 tttcttctgg aacagacatc aggaccccaa accatttttt gcttttttga tgtctgaata
     9301 cgcaataatc aggaaatttt ttaaaaatat attctggaga caaattgtat gtttcattga
     9361 tgtgatcgat tagttctttt ttcgttagca tctgcttcca tctctttctt attgattcgc
     9421 tttattcaaa gataccacgt aagaatagaa aaatgaatac aagtagttgc taatcaaata
     9481 cttcgatatt tttctttttt atcaactctt aaatgttcaa tccgcctgtg ttggtcttgc
     9541 taccatctac tattaaaatg ttgattattg tttgcccacc tattttttga ttttagtggt
     9601 atcatttttc aagttcatga tgaaattctt atcatcagta aatgctccag aaaaataaac
     9661 aatgattgaa aaatcagttg aaacttgacc accgtttatt ttctccgtat tcttagaata
     9721 gaagggagtc tgattgatgt attcactaat gaaaaaaatc attaccgaaa aagatattcg
     9781 acgtcatgtt tctttagtag aacaattgct gaatcataca aagataaccg taaatgaatt
     9841 agctgaaatc atagggacaa ccgaacgaac gattttttct gaccttcaat ctattcgctc
     9901 tcagctgccc gaaggctggg acatcatcag cgatcaagca ggaatcagtt tgcaaaacca
     9961 gcaaaattta ttaacaaatg acctttggga gatttttttc aaacaatcag tcagtgtcga
    10021 actgctgaaa aatctgcttt ttacaaaaaa agttgctgtc cctgattttc tagccgacca
    10081 tgggctttct tatgggacat tgaaaagaca tgtgactaaa atcaatcagc gtttagcaag
    10141 ttacgacttg cagatcgatt tgacgaaata taccgcttct attcttggaa aagagcgtgc
    10201 gattcgtact ttttatcatc gattgctgat tcctttcact cataacaact attttttcga
    10261 ggattattcg atccatgaat cccattattt tcaattttta agaaacctca gtcaaactga
    10321 actagcggtt gaaacagaag agattttcgg aacatgttgg ttctttatca atacgatccg
    10381 tataaaagcg aattgccgat tagattcttc tatccatacc aacagcacac tctcctccct
    10441 gtatgactct gcattgaaaa aattgtattt aacggaaggt atttatctta aagacactga
    10501 gctttctttt gcttctttct gctttctgga aagttggaat tacaacaata actatggaca
    10561 agaaaccgct cgctgcctgc atcattctcc atttttagag gtcctcgaaa cgtttgtaga
    10621 ggaactagct tcagagctgt ctcttgatca actaaaaaaa acatctctga ctgataactt
    10681 ggctctcctc atattaaaat atcatgaatc gccgatattg attgaagcat tggaccgtca
    10741 ataccatcgt tttttagaaa catatgatca gcaatatgca cacttgtatc aacagaaaga
    10801 gaatttactg aacgatctaa aaaaagaaat agaaatcaat gaaccaaatt actttctcca
    10861 actcttgtct ctactgatcc aacaaacgat tttttctatc aaaccgaatt tgttcaatgt
    10921 ctattttatt ttccaaggag aaccttcatg gaaggcgttc ttgcagcagg aattaaaaga
    10981 ttacttagga aaacgtgtct gcttcttacc tatcgaacta accgatcttt ccactatttc
    11041 ttttgaaaaa acagatatct tgatttccaa tttcccgcta gattctctag atatccctat
    11101 tgtatacatt tcttctattc caacgaaaaa tgagctcaat cgtttgactg aactcacttt
    11161 caaaactttt ttatagtaac ggctagcgac tgtattctct agctgttttt tcttttttgc
    11221 gaaacagttt gttcatgatc cctttttcta tttctacaac taagaataga agaatcacat
    11281 ttgctccaat caataactgt tgtgtcatcg ttaaacttac tgtaccgatc agctgctggc
    11341 cgactggtag aaaaataacc atcccttgca ataaagcaag gatacctaat gaaagaaaaa
    11401 gcgctttgtt ttgcaaaaga ccttggttca atgacggctt ggacaactct ctgcaattga
    11461 tcatgtaaac agcttgagct aagacgatat tttgcagaag aatggtttgc tgtaagcttt
    11521 gcccttcaaa ctgcatagcc aataaatagg caggaaccat gatcagtagg gagacataga
    11581 tgatccgaaa caaatgataa ccagatagga ttccttgctt tggatctctt ggtgggcgct
    11641 tcatgatttc tggatctggc gactcgaacc ctagtgcata agataaagtg attgtcgtta
    11701 ccatattgac ccataaaatc tgtactgggg acaaaggtag cgggcgattc agcataagtg
    11761 cccaaatcaa gatcaatcct tgagctaatg cagttggcaa gaagaaattg atggtttttt
    11821 gcaaattatc caaaatccgc cggccctctt ttacggcttt tgcaatcgta tggaaattat
    11881 catctcctaa aaccatatca gctgcctgtt tgcttacttc actacctttg attcccatag
    11941 caattcctac atcggatctt ttcaaagcag gtgcatcatt tacgccgtct ccggtcattc
    12001 cgacgatttc tccatttttc tgcaaagctt taacaattcg cagtttatgt tctggtgtcg
    12061 ttcgagcaaa gacatcgact attggcactt gctgaatcaa ctcttcatca gacataagat
    12121 ccaaatcgat cccttctaaa actttggcag tatgttttaa gccaatttgt tccgcaatcg
    12181 cttttgctgt gtctttgtga tcccctgtaa tcattttcac tgagatccct gcttgttggc
    12241 attcctttac agctttgact gcactttctt ttggcggatc gatcaatccg gcgatccctg
    12301 caaaagttaa ttgggttaaa gtctcatgcg tgagttcctg tttcgaatct acttttttat
    12361 aagcaaatcc taaaacacgc tgaccttttc tcgcaaattc tgcagctgtc tgactccatg
    12421 cttcttgttc tgcagaagaa agagttgatt tagcaaataa tacttctggt gcacctttga
    12481 cgtaaatcac cgctcctttt tcctccgcat gcatcgttgc catatatttg tattcagaac
    12541 tgaacggaat ctttgcttct atttttttca aaggtagatt ggctgtttct gtgtgtcgaa
    12601 tcagcgcaac ttctgtcgga ttgccactta aattttctgt tttctgttct tcttttgttt
    12661 ttaattcctg acagtttttc ataatggaca acacgcaggc ttcttctttt gcagctacat
    12721 ccacaactgt catttcattt tttgtcaatg ttcctgtctt atctgaacag atcactgtca
    12781 tagaacctaa tgtttctacc gaaggaagtc ctttgatgat cgccttttct tttgccattt
    12841 catgtactcc aacggataga atcatggtca aaacagctgg caatccttcg ggtaccattg
    12901 acaccaccaa agcaatcgta gccgaaagaa ggatatgcca atccatacca tatcgaaatg
    12961 tcgtaaaaat aactaagaat aggctcaaac aaagaatgcc ttgaaaaatt tgtttgttta
    13021 atcggttgat ttttttaatc agaggcgtcg tttgttgttc gaccgattgc aatgcttgat
    13081 tgattttacc gatttctgtc gtgtttcctg tttctacaac gacaccgact gcagaaccag
    13141 attgtacaag cgttcctgaa aaagccatat ttttctggtc gccgatcgat tcttcttctg
    13201 tcaaaacggc atgacttttt tcagatgctt ctgattctcc cgtcaaaatc gattcttcca
    13261 ctaataaatc atgtgcttcg attaatcgga tatctgctgg aacgacatct ccggcacgta
    13321 agacaatgac atctcctttt accagttctt tcgcagggat tttttcttgc atcccgtttg
    13381 atagtactat cgcttcttgt cccatcagct gcttcaaacc attcaatgat tcttccgctt
    13441 ttctttcttg ccagtaacta acgagtccat tgacgatcac tacgaataaa ataatacttc
    13501 cttcaatata ttcactagaa gcaaatttca aaaaagaggc aatgatcaac acgatcatca
    13561 gcagatcagt aaaatgcttt gctatttttt tccatcgttt tgtctgctgc cttgctttta
    13621 tttcattata tccttgatca gtcaggcgtt tctgtcgttc ttcttgcgac aagccttctg
    13681 ttgatgtttg atagtttttc aatacatctt ctaccgtcaa tctgtaccag ttcatcctat
    13741 cccctcctgt ttaattgata ctgttattat acgcagggaa gttttttcag gcgtatcgat
    13801 tatttcaggg gggtctgaaa taattcgttt tgcaaaatgt atctgggcct aactgatatt
    13861 tctattgctt attcttttat atacaaaatt taagaataca aaccagataa ccaataaaaa
    13921 aggaataaag cgtgtcgtgc tttcgaataa caagctgaaa gcaattgaag taaaaaacac
    13981 caacaaagca agatctttat atctttgtgt agtgttccgt ttcttttctt taataaattg
    14041 aacatgtgca cacaagatcg aagaccacac aaatagaaaa caactcgttg tcatgcctgc
    14101 caaaagctga aaaaacaatc gactatcagc tacaaaaaaa tgtagacaaa gaccggcaaa
    14161 aaataacatt cctgtaatta aaataccgtg taaaggtacg cctttcttag aaagcgcaca
    14221 aaaccaatgg ggcaaacttt tttcttttgc tgtttgatat aaaatcctgc tagtactgta
    14281 gactgcacta tttgctgagg acaaagaagc aaccaaaatc attagattga caattgctgc
    14341 tccgccttgg caatggatca cttgcagcat agtcactaaa ggactttctt ttaaatctaa
    14401 acaatgccat ggaacaacaa ataaaatcga caaaatcgtt cctatataga aaaagagaat
    14461 acgccaaggc agacagttga ttgctttttt caaattcttt tgcggatcac ttgtctcgcc
    14521 aacggttaag ccgatcattt caattccagc aaaactgaat aaaaccatag gaaatgagac
    14581 taccattcca gcaaagccaa acggtagtaa ttcatcgatc tgtccaaaat tttctttgat
    14641 tccgatagac tgggtatgaa aatctgagac actcaatcct atacccaata agataaaaag
    14701 gaaaatcccc aagatcttga tgattgagaa aaaagattcc aattcaccaa accacttgac
    14761 tgcaacaagg ttgactgcca ttaagatcat tagtgcgcta cctccgctta tcaaagctgg
    14821 tacatgtaca aaccagaaat ggatatacaa tcctattgct gttaattccg caagtccagc
    14881 tccaagccag caaagccaat aagtccaatt gacaaaaaat gcccactttt tgcctaaata
    14941 atagtcaacg aaatcactga tagagttaaa tgtactattt gctaaaaata aatcacccat
    15001 ggcccgcatc aaaccataaa tagccaaacc gcatatcaaa taaacgagaa taacagctgg
    15061 ccctgctgat gcaattgcct ttgctgaacc taaaaataat cctgttccaa ttgcgccacc
    15121 taatgcgatt aactggatat ttcgattagt tagagtccgt ttcattgccg gtttttccat
    15181 caagttcacc tttttctttt tcaagcagca tggcgacttc ctgttcccaa gaaggtttct
    15241 ttattttttc aatacagagt ggtactacaa acaaaatgac actgatggct gtgactgcaa
    15301 ttagattgat ccatgttcca gctttcatcg tcagtccgac agaaaatccg attgccgcaa
    15361 ataaaacaat aacgacagtc caaactccaa gattgctctt tttctctttt tttccaattc
    15421 taaatggtcg attcaacttt gcatgtgtat aacgcaatct caaaactgcg atccccatga
    15481 tgacatagac gatacagtag ataacagtag ttgaatttac gagcatcaaa aatgcttgat
    15541 tgacgccagg aatcagcaaa taaaaaagtg caaagattga gataatgaca gactgagtca
    15601 aaataacgga tttggatacg tcaaattgat tcgtcttcca aaaattgaat tttggtggat
    15661 acgcaccttt tctagcactt tctgtcactg tctttgctgg tcctgaggcc caagcagata
    15721 attggactgc tacaccaatg aagacaagta aactaaacag attcacaata agatgtggca
    15781 aaccaagaat ttggcaataa atcgaaatag attgcgcgat attattcaat tccatttgtc
    15841 cttttggtac gacatttgct acaactaaag cattcaaaat attgacgaaa aatataaaaa
    15901 ttaacgcggc aaatactccg cgaatatacg ttttaactgg attttccaat cgagtgatat
    15961 acacagagga catttcgata ccagtaaaaa taaacatgat cggcgcaaaa taaacaaatg
    16021 aggatgcagt cgtagtatct ggtattaatt tgtcccatga aaaactgcct aatgttccgg
    16081 ttggtacgat cccaactttg atccacgcag caaaccctaa taagagcatc atcgtcaaag
    16141 gcacgtacaa tcctaaccaa acaccgactt ttccgccgat ttttgccata tcaaatttca
    16201 aattcaaaaa agtgatgatc caataaacga ctaaaatgac aactaatgta aatttgctgt
    16261 tcaaaccaag cggcacatta tcgataatat taccaaacaa cggagcgagt gctgaagcga
    16321 ccatcaccat cccaggaaac atttggaccc ataacagcca tgagacaacg aacccccatt
    16381 tttttcctag agcattcgat acccaaagtt ctggaccacc tttttgggga aacattccag
    16441 caaattcacc agagatcaat gagattggaa aagcgtacaa taatactgca accagcatgt
    16501 aaaaaatcat cgtccagctc gttgctgcta catctggaat atttcgtaca cttgctacaa
    16561 gggcgcaggt cattccgata aaggtcatgg tcgtgatttg ctttgatgaa ccagtatctt
    16621 tcatctttat tccttctttc aatagcgaca tatgtttttg ttttctttta tttttgttca
    16681 agaacagaaa tgacaaggtt tcatcgattc agtacaaaac ccagtatcac cgaaacatcc
    16741 attgaacgat cttagtgaac tgggtttgat acatgaaacg aacgatccaa cttatagaac
    16801 gttttataaa aaagaatatc ttggcgagga atatatgaaa aacaggggga aatacaattg
    16861 ttttgatgaa gcaaataatt gtctgttctc catatgtctc ttgttattct ttcacacaaa
    16921 aatcagttct attggatgat ttcgtttagt ttcttttcca ttgctttggc gatttttttt
    16981 gtataaaaat tgaaaattcg atcatcattc aaataaggag tcatgatagc cgcacgtagc
    17041 agagtgattt tctgttcttt tttccattct tctttcgtta gacccattct ttcaacaaaa
    17101 cgaatcggag aatcaccgta atcttcttgt gtaaatgtag tatgagaagt aataaaccgt
    17161 tcaccataaa catcgccatc catataagaa gaccgatcga acattttttc atttaattca
    17221 ttgatagcgt tcaaatctgt acagccttgt tctttgaata cccaattgac catattaaaa
    17281 tcaggatgat tcaatggata gacttcgatt gtttttcctt taacagtaaa agtcagatga
    17341 tcgagaaatt ctctgaatct ttgagctgcc tcgattgaag ccccaatcaa ttgaccatat
    17401 ccggtcacat tcaatggcaa tacacgatgt gctgtccaga ctgcggcggc ggttgctcca
    17461 gcttttgatc cttccaaaat ataagctcct aacatatctg gagcctttac tgatttttca
    17521 aacacatatg gcgcgaaata agaaatgata tttctcatat ttttatgttt gattacgatt
    17581 cctccagcag cataagggac atagcccatc ttatgaggat ctatcgtgac agaatccgct
    17641 tctgaaatag ctctgaaccc ttcatatacc tctttgtcaa tcgttacgca gtggtggaaa
    17701 acatgatgtt cttcaaagaa ttcagctaag gaagcatacg gcacaaattc tcctgcttcg
    17761 tttagaaaca gtgaacgcgc atagccgcca tatgcggcat caacatgtaa atagaaatag
    17821 atcccttcat cttttaacct ttctcttaat tggacgattt tatctacact gtctacttga
    17881 ccttcttctg tcgttcctac gacagcaacc acacctagaa ttgggatttt ttgtccagct
    17941 aattcacgaa ttgttttttc caaagcatta atatccattc gataatcctc ttgaactggg
    18001 atagcaacca tctgatctaa gccgactccg caaatatcta acgccttcat ccaagaatag
    18061 tgttttgttt gagggaccag ccatttccct aatctttgga tattttttcc gctacgggaa
    18121 gaagctgctt tgacctcatc catttcttcg tccgtaaagc tttcggtcat ttccaaaatt
    18181 tcttcaacag ataaattcaa aagttcccac tcagacattt ttttcacttt ttcaggcagc
    18241 acttccttga cagctaaagg aatcgatttg atgcaacggg cgtaccataa tccttcaaga
    18301 ttagcaatag aaccatctgc tgcaatgtgt ccccatccgt ctgtcatatt gaataatgaa
    18361 gcaaaatcct gtcctacttc tgcttccatt tgcgaagttg ccatagaaga ttccaatgca
    18421 acattatttg ggttccacag catcgcatag ttgtaagcta ataaagcagg catcaatgtt
    18481 tcagcattca tttgtcccca gtaacggcct gctgaatgcc atgggatcga tcctgcgcgt
    18541 aatcgctgac tgacttcatc caagacctct atcatatggt cgcgggtggc tagataatct
    18601 ggagtgagct tatctccttc gctaattgca ggcatatctg aaggaatata attttctctc
    18661 catccaagat gttcatctac catcttgttc aacaacattt tataaactgg tccattttca
    18721 gctttgtcgc ctataaagac ggccttgata tccatatctt tcatttttct tccctccata
    18781 ttttgttggt caatgtcttg cagatacatg tgtttatctg taacacctct tcactatagc
    18841 gccaaaaatg agaaattttt ttttataaca tcaaatccac gtagattttt acaaacaaaa
    18901 ataaataaag tcggattttt ctgataattc aacgcaaaaa agcgaacaat ttttcaataa
    18961 atcagtcttc taaaatagat aacctaaaaa aagcgaacaa acaaataaaa tgactgtcct
    19021 tcattcacta aacatatttt tattttttta tattgttaaa aattcacaat ttatttttta
    19081 atagaattat ctgttttcca aattttataa cataaaaaac cttttttaac ggcatttata
    19141 tgctttttca gaaattaact gatgatttca aaaagaaaac atgtcataat atttcccata
    19201 ttgtgcaaaa aaatacaagg cggaggctct actatgattg aagaatatat tgagaaaaat
    19261 attttaagac agctgttttt atgtgggcaa ttctatgtaa ataaagaagt caatctagaa
    19321 aagctctcca atctcctgca tgtttgcaaa accactttac tcaatgatat caataacata
    19381 aaaaaagaat ttgaagaaca gatcgcttat acacatcggg aaaaggaccg ttacacttta
    19441 tatttttcag aacatatacc tcgatgcaag atcatgcagc aattgtctca aaattcgttg
    19501 tttttaaaaa cttgtctgct ttatttggaa gaagatgaac ctgattatct tcaattaact
    19561 gaatgtgaat tcatctctgt cagcaaagct tattcattaa aaaaacaagt attagcttac
    19621 tttaatgact gcgggatcga aatcgaccgc tactcccctc gattcactga aatggaaaga
    19681 cgtctgctgc ttcttaatgt gtcgtatcgt ttaggcggtt ttaattcttg ggagctacct
    19741 gaatcgtttt ttgaacgtgc cgatcgtttc attgaatccg taacggaaaa tagtggcaga
    19801 ttttatgata aagaaaataa agaaatctta tcgattggtt ttgctatcag tttcctacgc
    19861 cagcaagtgt gtgctgtgac aatagattca aaatttatcg aagaaatcaa aaaacgtcct
    19921 atttacaatt atgtcgaatc agcatgggaa aacactgatt tccaaaccta ttataaaaaa
    19981 gaagaattcg cctttatttt gacactattc aatctttgta attatggttt ccattcttat
    20041 cagttgattg cggaagactt tcaacagcta caccaagtgt ttattgacaa tactcccgaa
    20101 ataaaagaac tagttgctac ctttgaatct cacttcaatc aagaattatt cggaaaccaa
    20161 ccgttcgaac gtgcgctgat tcatctgatg cgttcggctt gggataacta tcaattattc
    20221 atgccagaaa aattttatct tttaaatgag gaacagacaa atttatataa agaagtccag
    20281 acaatttttt ccagctggtc cagccagtta ccttatgatc tacggttgaa tcctaattgc
    20341 atgcgggctt ttgtgatcga attgagcgga attctccgtc tgacaaaaga acatctgact
    20401 atctatatcg tcacaaattc ggatgttcat tatctgattt atcgggaagc cttggaagct
    20461 gtaactactt tcgattttca agttgcacca acaatttatt caagtatatc tgatataaag
    20521 aagtatgcgc agcagtcctc aaaccgggta ttatgtgaac gtacattata tacaccagat
    20581 gcggtccaat atgaaaatat tattcctatt tcgatcaata cgatcgatcg agctatcatt
    20641 tcagctgtgc aaaacaaata aggggctgtg acaaaaatca tgtcacagcc tcttatttgt
    20701 tgaagatgcc catttctgta acaatctctt gatacacgtc aggctcacaa cctcacttat
    20761 tttggacatc taggggtccg atctctttat ttaaaaaata agaaatgatg gttcggataa
    20821 ctaccaacgc cgctagttgg aaaatatcct gcaaggtagg tttgataatc gattcgatga
    20881 tcccagctga aacaagaatt tccaaactta gaagtatata gcctcccaac atctttttaa
    20941 tagcattgtt ctcgcttact cggtcgcttt gcacttttgt atggacaaat aaatatttca
    21001 aaaaatcctt gattgataga caaacacccc aaaccaataa acagatagca aagatatcaa
    21061 gtcccagtac aagtttttcg aaatatggaa gaagaaactg catcaactct gtcgtcatcc
    21121 tacctctcct cttctttgtt tttctttgtc ttctatttcc tgattcaaga aataagaaat
    21181 gactgttcga atgataataa taagtgcaag cttcaaaata tctgtccaag tcggcttgat
    21241 aactgactcg atgatatcag caacgactag tacttccaag cttaacaaaa tataacttgc
    21301 tagcagtttt cttgtatctg cattacgagt attacgttct gcatagtttt tcttgacatg
    21361 agaccaatca aaaagtttga atagaacaag aacgatcccg acaattaaga taccgataga
    21421 acaaatattt aaaatagcaa tgatccattc tagaaattca attaaatttg agcgaaacat
    21481 atacgctacc tactttcttt taatttgttc ctattctttt attgtaaaag aaagttacct
    21541 tgttcgtcta tttaaaagtt gttctaatga taacggttcg cttaaatttt tctttttcac
    21601 tctcccattt ttctccctcc atccataaat cgtggaagta tccaaaacaa cgtagcaagt
    21661 taaaataagg cttagtccgt catcagcttt tcaaagaagc actgtctcgg tgcttttttt
    21721 gtacccaaat ttctaaaaat agaaccatac acctcctcaa atcttttctt cttacctacc
    21781 ctatttttat ttacaggaaa atcagacact tcttcttttt tgtcaaaatt tcctttttct
    21841 tctcgtccta tcatttggca ttctaataaa aacgaactat ccttggatat agaaagagaa
    21901 aacaaggagg cgtttttcat gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg
    21961 cttgcccttg gcgctgcata tctaggaaaa aagacaggac tattcgaaga tgacagtcat
    22021 ttatatgatg aattcgaatc aatctagcat cctatttagt tgaatcacta cggacatact
    22081 tggaagacat tggaggaatc acaatgagaa aaagtaaagc aataatgatt ggtgccggta
    22141 ttgccaatat ggcagcggct gtttatttga ttcaagaagg caaatggaaa ggggatcaga
    22201 tcacctttta ttcattggat gatcacggtt caaatgatgg tgcaccgacc gacactgtag
    22261 tggatgaata ttggaataaa aaccatccat tagaaaacca aaaaggctat attgctcgag
    22321 gcggtcgcat gcttaattac cgtacctatg tagatttgat ggatctgtta agtcgaatac
    22381 cttctgttac tgagccaggg atgactgctg aagaagatac gagagatttt gatgcaaaac
    22441 accgaacctt tgacaaagca cgtttgatgg aaggtggcaa aggaatcatt gatggcggaa
    22501 aactgggatt caataataaa gaccgtattc ttctgactaa actgattgct atgcctgatt
    22561 cagaagaaga aaaactagac aatatcacta tcgaagaata tttcaaagat gatccacact
    22621 tttttgaaac aaacttctgg tttatgtggg aaacaacatt tgctttcaga acacgtagtt
    22681 ctgctcaaga attgcgtcgc tatatgcatc aaatgattta tgaattcact caaatcgaac
    22741 atctagtggg cgtcaatcga acacgttaca atcaatacga aagtattatg cttcctttga
    22801 tcgagtactt gaaagaacaa ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg
    22861 aattcaaaga aacaccgatg caagatgaaa tcactgtgac tggtttgaaa attaccaata
    22921 cactgactca agaagaagaa tacgttgtgg tcgatgaaga tacagcagtt attttcacta
    22981 atgggtccat tacagattca gctactttag gagattacga aacaccagct gtagaaaata
    23041 tggattatgg tgcagcttct agtctctgga aaaaagccag tgaacgtttc tacaacttgg
    23101 gaaatcctga caaattcttt gctgatcgag atgcaagcga atgggtcagc tttaccctga
    23161 cgacgaaaaa ccatttgttg ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa
    23221 atgccttgaa ttcatttatt tcaaccacac cgatcacgcc gttaggccaa aaagatgtca
    23281 atatgtctat tgtcgtgcat catcaaccgc attttacgtc tcagaaacca aatgaaacag
    23341 ttatttgggg ttatttcctt tatccaagac gacgtggcga atttgtggat aaacagtata
    23401 ttaaaatgaa tggaaaagaa atgctagaag aactgattgg acaactttca aaagtagatc
    23461 ctggaccagt gaatattcgt gaaaaagaaa cagaaatttt cgacagcgta ataaataata
    23521 tccctgtcta tatgccttat gcttccgcgt tgttcaataa tcgtgccaaa agcgatcgac
    23581 caaaagtcat tcctaaacat tcgaccaatc tggcctttac aggagaattt gtcgagcagc
    23641 cttatcagat gatctttact gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc
    23701 attttgcggg tattcctatg tcccgtcttg tcaaaacacc gcgttacgac aaagatattc
    23761 ctactctagc tcgtgccgca aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg
    23821 ccctgcatca agtaataaga acaaccaggc taaaacagct tcttatgttt ttgatttggt
    23881 tggacttatt accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag
    23941 ctgccctgca tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt
    24001 ggttagactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa
    24061 aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc
    24121 ataaatttga cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg
    24181 tctgtttctt tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc
    24241 atctaacgga tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct
    24301 ctccgaacac aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt
    24361 atcaaaaagg taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa
    24421 ttttatgaat ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa
    24481 aatggaaatc agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg
    24541 tatcacagca acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt
    24601 tttggaaaaa gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc
    24661 tcaagttcaa acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg
    24721 tcattgtcat ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca
    24781 gttatatggc gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc
    24841 ggaatatcta agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct
    24901 tcctgatgaa aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga
    24961 aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct
    25021 attcgctcaa aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt
    25081 acagcaccgt ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga
    25141 cacggcacaa gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat
    25201 cgattgtgta ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag
    25261 cttacaaagc ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg
    25321 aacagtccaa gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt
    25381 ttttatgcgg gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa
    25441 aatttacgca atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct
    25501 gccactatca gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat
    25561 aaaagttgtt ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa
    25621 aatcaaacga aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg
    25681 acgtcgcttc cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt
    25741 attaaccggg attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg
    25801 atatggcacc tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag
    25861 aatcgtaagg aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa
    25921 aaaagaggca tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt
    25981 tgatacagac gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg
    26041 tatcgttcat aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga
    26101 tgcatttctt ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc
    26161 ggttcaacaa acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt
    26221 tcaatgacgc ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga
    26281 ggaatacttt ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga
    26341 actccccatt ttgatgaaaa ccacttcgca ctttgtttta ctaattctgg gtgttctctt
    26401 aatttgatcc agttatatgt aaccaatgtt taaacctctg actaaaataa aacgtttaca
    26461 aaaattatac aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaac
    26521 ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt
    26581 ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa
    26641 ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt
    26701 ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca
    26761 aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg
    26821 ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca
    26881 atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct
    26941 aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt
    27001 ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg
    27061 gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag
    27121 aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt
    27181 cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat
    27241 gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat
    27301 gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt
    27361 tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata
    27421 aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa
    27481 atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag
    27541 aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct
    27601 ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag
    27661 ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag
    27721 tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt
    27781 ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta
    27841 cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa aagctatgga
    27901 tgtcatcaga tgatcttgac tgtggactgt caaaagcgtg acttcaacgt gatttccttg
    27961 tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc
    28021 atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc
    28081 cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt
    28141 tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt
    28201 ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata ttgattatat
    28261 ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt
    28321 gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag
    28381 cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg
    28441 tgactaggag acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca
    28501 ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt
    28561 aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca
    28621 ctaaaaaaga aagtgcacac taaacacaca aattaatttt tattaccttt tttcttttaa
    28681 cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact ttttttcgaa taaacggtat
    28741 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt
    28801 tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg
    28861 tgttcaatca gctgaccctg gattttagct caaaacgctg gattcacaac ctcttttatt
    28921 tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc
    28981 gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt
    29041 caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa
    29101 tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc
    29161 gccttcttct gttgctttga tcaaataacg caaatgctgt ccgataaatt cgatacggta
    29221 atcatcttgc acgattcctt tctcatccat ataccgttct tcatgttctc ttcccatacc
    29281 gttttctgtt atcagccaag ggatattatg ataattttct tttagataca tggctatatc
    29341 atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa
    29401 atcaaagact tcataatatc gttctggatg gaaaggtgtc tcttcattcc attgataacg
    29461 aggagcgcga acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg
    29521 ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa
    29581 ctccaggaac tcttctgtgt agtacccttt taccatcgga tcaaaaaata ctcgattata
    29641 aaacaaatca tacatataag cagctttttg gtcttctgga gaattagatc gagcatagac
    29701 catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc
    29761 tactgcttta gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt
    29821 ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc
    29881 gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata
    29941 cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa
    30001 agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga
    30061 gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc
    30121 agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat
    30181 cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc
    30241 attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc
    30301 tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt
    30361 ttttggaata gtaattttca tgatttcctc ctgtttttag atcccatcat tttccacacg
    30421 atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac aaacaagttg
    30481 cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga tcggcggcat
    30541 tggccaaggg accattacaa ctgctttcgc cgcaaaccct aaagcggttg caaaataacc
    30601 aatcgttcct gtgataagag gcgttaagat aaacggaata gctaaaattg gattcagcat
    30661 gatcggcata ccaaaaataa ctggttcatt aatgttgaag atcccaggag caagggataa
    30721 cttggtgatt tgtttcatat cctcccgttt tccaactaac aatactgcaa tcagtaaacc
    30781 gatcgtgact ccagacccac ccatactcat atacatatcc caaaatggca tagtaataat
    30841 gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag
    30901 caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca cccaaaagac
    30961 ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgaacgacac cttctaatgg
    31021 gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt ctttgatgat
    31081 aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag cagaaaaact
    31141 agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct tctgtttcct
    31201 caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa ataacccttt
    31261 cgtgtctgta tattgtttag ccaatacgtt ttctactata acagtctgat tttcattcac
    31321 taattcataa acagttggat taacagataa ataagacatc actgcgatga taccagggaa
    31381 tacaccatcc actttattca gcttggctaa ttcgatccct attagaaaaa cagcatagat
    31441 ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta attcttctaa
    31501 aaaacgcaag taacttatct gtgctaagcc attttccgag tcaaagacca ttgctgaaaa
    31561 caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat
    31621 caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa ctttggctaa
    31681 tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt gacgtcatct
    31741 ctttttgtct attagtcagc gcttacattt tggtgaacag aaactattta ctcctctttt
    31801 ttaccaaata taaaacaaaa aatcatctga tgtatttatc ataaaataca tcagatgaac
    31861 tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt tattgccata
    31921 tggacagtca ttgcgtcaaa cgcagcaagt acgttccgcc tttcgatggc ctgaacgatc
    31981 tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt tttattaaac
    32041 aaagcaatcg attcattaat gattgggata atatgcagca aggctatgtt tccgctagct
    32101 tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt gccttctcga
    32161 aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt cttcgttgca
    32221 tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc aaataagtct
    32281 cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct
    32341 cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt cctggaagct
    32401 agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc ggacaattca
    32461 tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata tctaaggatc
    32521 ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca
    32581 tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat
    32641 aaaaaatcga ttaatccgtt catagacata caaacgaaga agatatttac cataaaaaag
    32701 actaggataa aattcactcc tagccttcga tcatttttat tttgatcgac aaaaaatatg
    32761 atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt ttttttaata
    32821 tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat tctcaattct
    32881 tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa ctgacttaac
    32941 cgacttgctc cgatcagtac acttgtaaca gatttttgtc gtaaattcca tgcaagggct
    33001 atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt gattttttcc
    33061 agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg tgctctagaa
    33121 tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag cggactgaac
    33181 gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc tagtagtgca
    33241 cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt tctttttaaa
    33301 atttcagtca tttttttgta tcttctccat tatagttgga tataccaata tataatgctt
    33361 ttccttgtct tacaagttgc atcaacgctt ctgctgtttc ttccaaaggt gtatcagggt
    33421 ctgggcgatg atgatagaag atgtcaacat aatctagccc cattctttgg aaactttggt
    33481 cacaacttga tatgatggat ttacgagagc cccattcccc atatggccct tcccacatat
    33541 gataacctgc tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa
    33601 aaatacgccc aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt
    33661 cgaaatgggt aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag
    33721 gatcataatc accaaaattt tgccataatc cgagaggaga gataaaatcg gtaatttcaa
    33781 acctgaatga ccggatttac gatattccat tttttcataa cgtgcactat ctgcaatata
    33841 caatttttct cctcctcatc aaatcttttt aaaacgcttt cttgttttct atttttagtt
    33901 tactacattt taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat
    33961 ttgtttttta ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttatag
    34021 cgtttcggtt gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg
    34081 gctggttcga tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt
    34141 tttgattgtt ccgctaattc tttttgtcgt tcgtcttgat aaaaaatcac tggacgatat
    34201 tgcttccctc tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag
    34261 attgtcagca actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc
    34321 acatgacctg tgtaaccacc acttacttga tcataattcg gtttttcaac atgtccgccc
    34381 gtgtatcctg acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac
    34441 cagaaacatc cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc
    34501 ttggaaaagt catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc
    34561 attacatcag gcgtcagatt cattcggata gctaatggac gtagtgttac ttctagtttt
    34621 gataactgtt ctttaacgga tatattttct cttgtgtcat tctttgtctg aatcaatact
    34681 tttcgttccc aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat
    34741 atttgttcct catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc
    34801 tgtaatttat cataaagtga ggaaataatc gattgaatcg tatttattca aagaagataa
    34861 gcagcttcag atgatataag atgatcttta tcttaaaaag catactctat ggtttggttt
    34921 aactaaaaaa cgacctctga aaaaagggat gttctccaat tctcagaagt cgagtaaaga
    34981 tgattctttt ttatgagcga taataaactt catccaaaaa cagaccttgt gcagggacgg
    35041 tttctcctgc atgttgccgc actttgcttt caaatatttc atcgattgtt tctatcggca
    35101 tggcacctgt gccgatttct agtaatgtcc ccatgataat ccgcaccatt ttatataaga
    35161 atccgtctcc gacaaaagta aaatgaagca tttctccctt tcgttcgata gataattcat
    35221 caatcgtacg cactgtggat ttttttgatt ttttcaatgc agaaaaacca atgaaatcat
    35281 gtgtacccac taatttttcg caagctgcat tcagcttgtc catatccaac tcttgaggga
    35341 aataaaagct gtggtttcgc tcaaatgctg aaggaatcac gttgttccaa acataatagc
    35401 tgtatttttt gcccaccgca ttatatcttg aatggaagcg ctcaggcatt tcttcgattc
    35461 ttttgatgac aatatcccgc ggaaggtaac gattcataaa gtcgatcatt tctgccagtc
    35521 caatttctgt gtttgtttta aaatttgcta cttgtcctcg cgcatgggtg ccagcatctg
    35581 ttcttcctga tccaataatt tctatctctt catttgtcat ctgatgcaat actttttcga
    35641 tttttccttg aatggtttta tcgtcatctc caagtctttg ccatcctgaa tatctttttc
    35701 cgtcatattc gattgtcaac ttgatgtttc tcatatttta gtggctcctc tgtcttccat
    35761 ttggtcttct ctctaccttt ttaagattgt agcacaaaat atagctcctt gcttctctaa
    35821 gacagaagct atacaagaaa actgtggaaa tatatatttc agaaaaacaa aaaacagcca
    35881 ccggatgaag tatccgctga ctgttcaatt tgacggctca agttttactg tttgagatgc
    35941 aagatactca accaggcaat gggcacataa ttaaagaaaa aactgattct ttggcaaaaa
    36001 cctattttat tcagtaatgg ccaagaattt atagaaggaa ttcttgccag tccataaatc
    36061 gccgcaaaaa gcaaactgat cacagacagt ccaaggtata agtggcttac gtccatttgc
    36121 ccattttttc gatagatcaa gaaaagaaga actggaaaca ataaaaaacc actgtaacct
    36181 aaacctgatc cgatattatg gacccatgtc gagacattcc acgaagattc atgggtattg
    36241 atactgaaca aacctgtaaa gatacaatca cctataccat agaacgcgat ggctgctgta
    36301 actagggtag ctaacacacg agatgtactt acaaatgcag catacaatgc aggcaatgac
    36361 aggatgaaaa agatccctga aaccacagac caaataagaa acgcttttct tacaggactg
    36421 gaaacctctc caaatacact gatcacgctc ttcatttgat ctaattcagg ataatacaat
    36481 cctaatacgt acggagtcaa aaaatcactc agcacgccta ataataaaaa gaaaaaacca
    36541 aatttacgta aaaaatccat gatacgcctc caataacaat tacctatgct tttaattgtt
    36601 attataaacg actttatctt gatttaacag aaacctctat ttacacgaac atctatataa
    36661 ctcattaaaa actcgaatca ttttaaaaat actaaatatc taacgttcaa cgttcactta
    36721 atccatctaa acaatctttc aatttgattt tttccccact tcttttacgc gcgcctagct
    36781 ctcctattgc agtaaacaac agatcacttg acgaatttaa ggcagtttcc attgaatcct
    36841 ggataacacc gataataaat ccaataccaa cgatttgcat cgcgacatca ttagagatac
    36901 caaacaaact acatgctaat ggaatcaata acagtgaacc gcccgcaaca cctgaagctc
    36961 cacaagcagc taaagcagac aaaatacata aaaggacttt caaaacaaat ggtgcttcaa
    37021 tccctaaaga atgtacagca gtcaacgtca tcaaggaaat ggtaatcgcg gcaccgccca
    37081 tattgatcgt agcacctaac gggatcgata tggaataaga ctcttttgtc aatttcaatc
    37141 tttcagccaa catcatgttt atcgggatat ttgcggcaga acttcgggta aagaaagcgg
    37201 gaagtgcgct ttctttcaaa caaaagaaaa tcaatggata gggattttct ttaataaaca
    37261 aaaacgtgag gaatgggtac acaactaatg cgacaaacag catcgtaccg atcaaaacaa
    37321 gtaaaagctg cagatactct gccaatccag cgattccagt cgtagcaatt gattgataaa
    37381 ccaatcctaa aatccctatc ggagcaaatg caataatcac ttgtacgact tgtgacaacg
    37441 ctatagatat ctgatccacc aactcttttg tcgattctga acgtgctcga aaagctagtc
    37501 ccaacaatac agcccagaat aaaacgccaa ggtaattcgc atcaataaga gattgaacag
    37561 gattttgtgt gacattcgtc aacatagtag ataaaacact ttccaaactt tgcggtgctt
    37621 tttcagatac tgtttcttgt aaagtaatag gaacagtaaa aattgaactt acaatgactg
    37681 ctacaagtgc agaaagcaat gttcccacta aatataagat caaaataggc ttcacgaatg
    37741 tcttattccc aattttatgc ttggcaattg acgccatcgt caagaaaaaa accagaatag
    37801 gtgcgattgc cttcaagcaa ctgacaaaca acgtccccaa tattgaaata aatgtccacg
    37861 tggggaaaaa gataccaaac aatattccga ctaaaatacc aacaacaata cgttgaacta
    37921 acgacatatc caaaaacttc tctaacaaat gaccaccctt tcacaatttt aacaatcatt
    37981 gtatcatttt atctaaagaa actaaaggtg caattttcta aaaattctaa taatacaatt
    38041 ggcaattcat gctcttgcaa cattcaattt tagtattgga gggtttattc aatctctaaa
    38101 gaagtcgaga gaaaactatt tatcgttatt attatagtta tccaagtaaa acgtaactaa
    38161 gatgaaggac gaaactagaa atttcatcct tcatcttaat tcttagacat agcaatcaat
    38221 taatattttc cactgagtct cttctttctc tcactctcac tggtttactt acccataaac
    38281 gatttgatta aagccatttt tcaattcatt attcgctgtc tcgttaaagt aaagataatt
    38341 atcgggattc ccctgatttt gttgtgtcac caatactttt ggctgatttt gctgaaaccc
    38401 gaatagatag acgttctttt tcagatatgg ttctgtctct gcatcggaat agacggctgt
    38461 gatttgatag tctgcttgac ctgtcccact ttctgaccat tctgcagaaa caggcactcc
    38521 accaatagcc atcttccact ccccgttcaa aataacttga gggactttaa gaccatataa
    38581 gtccacactc atttgattcg tatagctttt gtattgttgt cccattgttt ggccccatgt
    38641 agccataaat gcctctaatt gtgcggattt gtctgcattc cacagtgttt gttcgacttg
    38701 ttcgctgctt tctgaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt
    38761 ttcagtcgtt ttggaagact ctgacgaagt agaggtatct gctgtttccg tacttgctgc
    38821 agttggcttg ctctctgtgc tagttgttgt agtagttgca attgttgctt tcgctgtttc
    38881 ttttgttgat tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag
    38941 accaattgtt actaaaatcc tttttttaaa ttaaaaaact cctttcgatt actcccactt
    39001 ctttattcta tcaaataagc aatgtgattt tattgatttt ttgtattttc tcatccatct
    39061 tttaaacc
//
LOCUS       DACDLI010000017        38394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDLI010000017 DACDLI010000000
VERSION     DACDLI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.017"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(314..1537)
                     /locus_tag="IV353_000966"
     CDS             complement(314..1537)
                     /locus_tag="IV353_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9009236.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(1568..2407)
                     /locus_tag="IV353_000967"
     CDS             complement(1568..2407)
                     /locus_tag="IV353_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9009237.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(2424..3104)
                     /locus_tag="IV353_000968"
     CDS             complement(2424..3104)
                     /locus_tag="IV353_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009238.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(3106..4179)
                     /locus_tag="IV353_000969"
     CDS             complement(3106..4179)
                     /locus_tag="IV353_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9009239.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(4826..5497)
                     /locus_tag="IV353_000970"
     CDS             complement(4826..5497)
                     /locus_tag="IV353_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9009240.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(5584..8262)
                     /gene="mgtA"
                     /locus_tag="IV353_000971"
     CDS             complement(5584..8262)
                     /gene="mgtA"
                     /locus_tag="IV353_000971"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9009241.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            8921..9358
                     /gene="ndk"
                     /locus_tag="IV353_000972"
     CDS             8921..9358
                     /gene="ndk"
                     /locus_tag="IV353_000972"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9009242.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(9380..10246)
                     /locus_tag="IV353_000973"
     CDS             complement(9380..10246)
                     /locus_tag="IV353_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9009243.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(10406..12499)
                     /locus_tag="IV353_000974"
     CDS             complement(10406..12499)
                     /locus_tag="IV353_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9009244.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(13248..13871)
                     /locus_tag="IV353_000975"
     CDS             complement(13248..13871)
                     /locus_tag="IV353_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009245.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(14030..15427)
                     /locus_tag="IV353_000976"
     CDS             complement(14030..15427)
                     /locus_tag="IV353_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9009246.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(15459..16430)
                     /locus_tag="IV353_000977"
     CDS             complement(15459..16430)
                     /locus_tag="IV353_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9009247.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            16804..17229
                     /locus_tag="IV353_000978"
     CDS             16804..17229
                     /locus_tag="IV353_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009248.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(17717..18418)
                     /locus_tag="IV353_000979"
     CDS             complement(17717..18418)
                     /locus_tag="IV353_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9009249.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(18434..18874)
                     /locus_tag="IV353_000980"
     CDS             complement(18434..18874)
                     /locus_tag="IV353_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9009250.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(18874..19449)
                     /locus_tag="IV353_000981"
     CDS             complement(18874..19449)
                     /locus_tag="IV353_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9009251.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(20297..20776)
                     /locus_tag="IV353_000982"
                     /pseudo
     CDS             complement(20297..20776)
                     /locus_tag="IV353_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(20935..21636)
                     /locus_tag="IV353_000983"
     CDS             complement(20935..21636)
                     /locus_tag="IV353_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9009252.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(21744..23153)
                     /locus_tag="IV353_000984"
     CDS             complement(21744..23153)
                     /locus_tag="IV353_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9009253.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(23201..25066)
                     /locus_tag="IV353_000985"
     CDS             complement(23201..25066)
                     /locus_tag="IV353_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9009254.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(25187..26038)
                     /locus_tag="IV353_000986"
     CDS             complement(25187..26038)
                     /locus_tag="IV353_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9009255.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(26348..27061)
                     /locus_tag="IV353_000987"
     CDS             complement(26348..27061)
                     /locus_tag="IV353_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9009256.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(27129..27455)
                     /locus_tag="IV353_000988"
     CDS             complement(27129..27455)
                     /locus_tag="IV353_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9009257.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(27578..27805)
                     /locus_tag="IV353_000989"
     CDS             complement(27578..27805)
                     /locus_tag="IV353_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9009258.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(28127..29380)
                     /locus_tag="IV353_000990"
     CDS             complement(28127..29380)
                     /locus_tag="IV353_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9009259.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYGNAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            29441..30181
                     /locus_tag="IV353_000991"
     CDS             29441..30181
                     /locus_tag="IV353_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9009260.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(30267..31025)
                     /locus_tag="IV353_000992"
     CDS             complement(30267..31025)
                     /locus_tag="IV353_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9009261.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(31022..32050)
                     /locus_tag="IV353_000993"
                     /pseudo
     CDS             complement(31022..32050)
                     /locus_tag="IV353_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(32070..35237)
                     /locus_tag="IV353_000994"
     CDS             complement(32070..35237)
                     /locus_tag="IV353_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009262.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVDVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(35461..36345)
                     /locus_tag="IV353_000995"
     CDS             complement(35461..36345)
                     /locus_tag="IV353_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9009263.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(36440..36877)
                     /locus_tag="IV353_000996"
     CDS             complement(36440..36877)
                     /locus_tag="IV353_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009264.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(37094..38005)
                     /locus_tag="IV353_000997"
     CDS             complement(37094..38005)
                     /locus_tag="IV353_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9009265.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT        10923 a         8138 c         6392 g        12941 t
ORIGIN      
        1 tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt accaccttaa
       61 ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga ctttccatgt
      121 aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa caaaacacct
      181 aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag atcatttgtc
      241 aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact ttcacacaca
      301 agtttatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact atttcttgat
      361 gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga gctgtattgg
      421 cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta gcagcagctt
      481 tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta ggctggattg
      541 ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt ctttgttccg
      601 tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat aagtcatcaa
      661 aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac tcaaaagtcg
      721 tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat actttatcta
      781 atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct ggaatgacgt
      841 tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt ggcaattgca
      901 agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca tttactcctt
      961 tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg actggatcat
     1021 tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat aacgctacat
     1081 cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg ttctcgcccc
     1141 cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca atgaattgct
     1201 tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg catgcatgac
     1261 caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg ggtttattgg
     1321 aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct acccaaaacc
     1381 tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag ttacttactt
     1441 caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga tattggatta
     1501 gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc tatgctagtc
     1561 atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct tttgatgttt
     1621 tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt tgatacgttt
     1681 tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt gcatggtcat
     1741 ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct acagctacac
     1801 cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg gaagcatcta
     1861 attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg ggcgcttgtc
     1921 cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga gctctgccgc
     1981 catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca gctacatcct
     2041 tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc gatactaaat
     2101 cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa gaattcaaat
     2161 caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat ttcacgtatt
     2221 ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca tctgtatctt
     2281 ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct gcttttcctt
     2341 ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa cgaataattt
     2401 ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg attactgata
     2461 aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt tacatctgtt
     2521 tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc acctactgct
     2581 ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac ccgaatgata
     2641 ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact ggttcccatc
     2701 gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc gatttgacga
     2761 gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc accaatcgtc
     2821 gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat gaaagggatg
     2881 gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt agctagtaat
     2941 ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat cccgccaatg
     3001 aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa ttcatccgga
     3061 atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc ttcctccttc
     3121 gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt ttacaccaga
     3181 tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta aagtaacgat
     3241 caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa tattcgctgt
     3301 tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat ttccaatata
     3361 acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat gggcaatgat
     3421 cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg tcaattcttc
     3481 ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc catcttccat
     3541 aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga tcaggacgat
     3601 tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa gggtcgtttt
     3661 cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat tcgccaaagc
     3721 tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat aagcagatgc
     3781 tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac gttcctgttt
     3841 cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc ggctttccat
     3901 tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca attctttggg
     3961 agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg gtttctgcag
     4021 caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga cgattccata
     4081 aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct ctttttcttt
     4141 ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc cttatctaaa
     4201 aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt tgcgtgttac
     4261 cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc atactctagc
     4321 aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat tgctcgatgg
     4381 ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct gtcaaaggtt
     4441 cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc cttttactaa
     4501 cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc ggaagtttcc
     4561 ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg gtcgaacagt
     4621 tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc ttttcttact
     4681 tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt tccttttcaa
     4741 cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa agtcaagtac
     4801 gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac atttcaataa
     4861 cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct tcgacggtat
     4921 aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct cgtgaagcag
     4981 ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct aatcctgttc
     5041 gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact tcgtctccga
     5101 atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct gttgctcttt
     5161 tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag tctgccaata
     5221 tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga taaaaatatt
     5281 cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct ttcactaaaa
     5341 tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct tttgggatat
     5401 ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt tctgtgtcaa
     5461 ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc ctttccaaaa
     5521 caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa ccagccttct
     5581 cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg tcacaagcgc
     5641 cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa gtggcatcaa
     5701 gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa ttcctaatgt
     5761 agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt tcggtgtacg
     5821 cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc ctgcatggaa
     5881 taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc cgcccactac
     5941 agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata tcgaacttgt
     6001 tggaccaaag taaaccatga attccccaat actagatgca ttccattttt ttggctcgtc
     6061 caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat catagatcag
     6121 attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg tacttgcaat
     6181 cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat atttcatgat
     6241 attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca aatctttttc
     6301 taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta cagaaatacc
     6361 gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa atccgacagt
     6421 atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat tcaattttac
     6481 gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt ccgagatttt
     6541 ctcaccggta accaattcat tgatctctag acctacttga cggcacactg aacgtgtaac
     6601 taattcgttg tccccagtca gaacttttac agctacacca tgttctttca gagctttcaa
     6661 cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa tcaaaaccat
     6721 gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct tttgtgcgac
     6781 accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta cggcttcttt
     6841 tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag aaatgttcag
     6901 catttcttca atcgcgcctt ttgtgatcat ttgtgtttta ccagctggat cagcaaccac
     6961 aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt gataattgat
     7021 cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta acaaattttt
     7081 cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat catcttcatt
     7141 tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg tccccgtttt
     7201 atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat ttttgatcac
     7261 tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa cgatcattgg
     7321 aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag cttctagcca
     7381 atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg acataaattt
     7441 gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa ttggtttttc
     7501 agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa caatgattcc
     7561 ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt aacttgtttc
     7621 gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc tttcccctgt
     7681 caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat ccgctggtat
     7741 catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca taggaatctc
     7801 tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt tcagtttttc
     7861 agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga cgataaccat
     7921 agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta atacatattc
     7981 cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg tgatgtatgc
     8041 tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat tttcaccgta
     8101 attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg catggaatag
     8161 ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt gtgtatcttt
     8221 ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc gctgcttttg
     8281 gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag aacagataga
     8341 taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat ccatttgttc
     8401 tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg aatagttatt
     8461 tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg caacaaaaaa
     8521 acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa gcttctcaga
     8581 tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct tttagctacc
     8641 ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct cgacgttttt
     8701 gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg gttttccttc
     8761 gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt tcatactttt
     8821 tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca ttttccacct
     8881 cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa cacttgttat
     8941 tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac gttttgaagc
     9001 aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat tagcaaaaga
     9061 acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt acatgacttc
     9121 cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg tccgtaagat
     9181 ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag actacgcgct
     9241 gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg taaaagagat
     9301 cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg cgaagtgata
     9361 caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt tttttctcta
     9421 aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat tcttggtagt
     9481 ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa tctgccatca
     9541 atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc atatcttcat
     9601 tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc aggttcatta
     9661 aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc attctttttt
     9721 tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga aaatcagcta
     9781 gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct tcaatgattc
     9841 ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt tcgaccatgt
     9901 gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta cggttattgc
     9961 tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt cgtctttttt
    10021 tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct gcaagtgtca
    10081 tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc actgaatttg
    10141 tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg gactgtgtca
    10201 acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata gttctccttt
    10261 ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa atacacgaaa
    10321 aaaataaaaa aatgtttttt tcataaaaaa taccaacctt caagcattag aatgttgtct
    10381 aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca acgtctgttc
    10441 ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag acacttgtcg
    10501 aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt ttatagtcat
    10561 attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag agattttcct
    10621 gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct ttggagattt
    10681 cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta ttgtaagctt
    10741 ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa gcaactactt
    10801 gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta tcatcgattc
    10861 ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca tgattgactg
    10921 tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc tgtgcgttgt
    10981 caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct ttattatctg
    11041 tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg taaataccag
    11101 attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt gccgttcgaa
    11161 cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa ttcttgtcat
    11221 ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac ggctgttgta
    11281 gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc ccataaccta
    11341 acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa cctaagtttt
    11401 tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca gcgcttgtat
    11461 agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct gacccaagtt
    11521 ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata ttttcagcat
    11581 cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc gttgcttgat
    11641 tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc atattaatca
    11701 aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta gcgatcccaa
    11761 atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact tcatcaatcc
    11821 cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt ccgtcataaa
    11881 tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat gttcgctgca
    11941 gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata agccctgaaa
    12001 gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata ggtattttct
    12061 tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag tagatgacat
    12121 catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact ttcgaaaagc
    12181 ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata atattcaagt
    12241 acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct ggctttggct
    12301 ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg ttgatccaca
    12361 agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag taggcaatga
    12421 tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga ttttggcaca
    12481 attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc ttattttgct
    12541 cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta ttctttctaa
    12601 agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga ttaaactaat
    12661 gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag ccagtttctc
    12721 ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct tatttcttga
    12781 tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca actcctcggc
    12841 cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt ccttgtggct
    12901 cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccctcg
    12961 gtattagatt atgtcaggac gctaatttta caacctcttg gtaaacggtg ttcaaaagct
    13021 tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctattt tttgaaattg
    13081 ctccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc
    13141 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
    13201 tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca ttgtccatag
    13261 gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt ttcttcactt
    13321 cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga ctcgtttttt
    13381 tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc cgctaaacga
    13441 tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt tccatttttc
    13501 aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg cttgtcttta
    13561 aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat aaattgattt
    13621 tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt ggcagttatt
    13681 ttatactccg cccaccaata agttgagaat tcttttttga aattatctac tgattctttg
    13741 cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt tcctgaaaat
    13801 gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag aaacggtctt
    13861 tttttcctca tgctttcctc cactcctatt taattttgac tattctatta tatacaaaaa
    13921 atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt ctttttcaca
    13981 aaatggtaag aatggagaat ttcacgcatt tgctctcaac tattctttct taagcctcaa
    14041 atccgttttt atctgatacg tctttgaacc aatgacccga ttttttgatt gttctttttc
    14101 catcttgtgc taagtctact gaaataaagc catatcgatt cttatatgca ttggtccacg
    14161 accagttatc catacatgtc cacatatgat agcctaaaca attggcacct tcttgaatgg
    14221 cttgatggac atatttcaga tgagattgga caaattcgat ccgataatca tcgtggatca
    14281 tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt tcagaaataa
    14341 agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag attccttgtt
    14401 catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga
    14461 aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt gcttttaccc
    14521 ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg ataaccgtca
    14581 aatcctctgc atggatcaca ggcatgagat caagttcttt tactatctca atcaattcac
    14641 ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta
    14701 tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt ggtgtcagat
    14761 tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg atggctaacg
    14821 ctgaagccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct gactcttctg
    14881 gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca ttatgagtaa
    14941 accatttttt tactttcgaa ccaaacaatt caaaacaact tttggcatat ttttcatagg
    15001 cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga
    15061 aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca aaataacgat
    15121 gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc
    15181 attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt
    15241 gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg
    15301 gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcgcct gaaatccttc
    15361 cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga aattgatatt
    15421 ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc atgtatgata
    15481 ttttccatca caatcacttt ttcattttct tcctcttctt ttttgattcg ttttaacagc
    15541 tgttctgctg ctcgttcccc gattttatct gttggtggcg tgatcgtgat tttcggaagg
    15601 atactcagat cgtttttatc taaataatca tagtgactga acgcaatatc cttcccgatt
    15661 ctgacacctt cttgattgaa acattcgatg gcaccaatcg acatattgta attgctgaca
    15721 aaaatagcag ttgccccttt ttctaataat gcttttgccc cgtaatagcc gctttttcta
    15781 gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttggac cgcttctttg
    15841 gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat aaatccgatt
    15901 tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc tcgatcattc
    15961 actaatattg tatcaatacc tgctggtcct tctggacaat tcaatgcgat aactggaata
    16021 ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa gacaatcagc
    16081 ccatcgatca catgcttcat caggtattca attttttgtt caattttttc tggctgatcg
    16141 gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc gatccctgag
    16201 acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat agagaagctt
    16261 cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc tgaaatcgct
    16321 tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta acgggaaact
    16381 gttcccactg aaacaccagc catcttagcc acatcacgga tcgatgccat acattctcac
    16441 tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac cgcattcttt
    16501 gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga gcaacttcct
    16561 ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat atagcatttt
    16621 tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt ttttgccttt
    16681 ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt ttctcttcca
    16741 tccgtttgaa gttttacatt ccaatggtat aatttcttta tcctatcaaa tgggggaagt
    16801 attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac attgactggc
    16861 tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc ttctgtctta
    16921 aaagcggaaa aagcagatac caccaacagt gtatatacgc taaaagtacg taaagatatg
    16981 gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac acgcacgtat
    17041 cagacacaag gaactgattc ttctgaatca tctgaagaaa aaacagtgct gacagatatc
    17101 aagatcacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa aaaagaaaca
    17161 acagtcatat ttacaaaaat cggtaattac cgtttgaagg atgaggatgg caacatctac
    17221 agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc tgctatagta
    17281 aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg attattgtac
    17341 atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct tgagcatatg
    17401 cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt atcctgttca
    17461 aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact atggctgatt
    17521 tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa aacaaacagt
    17581 ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat atttgaatat
    17641 aaaataacca aacgatagaa aggcttattc tacatcacgt agtaattttt ctttcccgtc
    17701 gtttggtttt ttatttttag cctaattctt gaccttttgc atgctggtta tccgtaaaat
    17761 attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga cggatcacct
    17821 tttttgcatc ttctggattt tctacaaaat cgaatttatc cccatccact tgtattttag
    17881 gacatacatc aaaatcttca taccattgat tatagcgggt gttcaacgtt tcataatagg
    17941 tatataacgt ctcatcgctt tctagctgtt catagtcacg cccacgtttt ttgatacgtt
    18001 ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggccgt ttttttggag
    18061 caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc tgtacttcga
    18121 tatccgttac ccgacctaaa tcagcgttta gatggaacaa caggctatct tcatagatgg
    18181 atcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa aatcgcttgt
    18241 tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg atttgaataa aagagcggca
    18301 atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct tttgcaagca
    18361 tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc actgacatac
    18421 aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat gtttcaaaat
    18481 catattcacg tacagcttgg tctgatttta aatacgtatg gagactttgt gtaatcatca
    18541 agttcgtagg taccgtctct tcttgtacaa tgatcatcgg ttttttcaac atagttgcca
    18601 cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca aggatagcaa
    18661 caacaaattc tgcatttgtc agttcttcca tatctttttc gtatacttcc tgcgcccatc
    18721 cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag aaacttgtga
    18781 ctgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt tgctcctctg
    18841 aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca ataataactg
    18901 atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt tggaaaagat
    18961 tacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt ctaataattg
    19021 ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttgt cgggcgtcag
    19081 accgattgct atccaagaaa gaagttgtga gaattcctcc aatgtaccta gacctccagg
    19141 gaaaacgagg acagcatctc ccttttcaaa taataatacc tttctttcat cgatcgtttc
    19201 tgtaggaata aatgtagtaa catcttttcc aggcaattca gtttcaaaaa gcccattcgg
    19261 atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca ctcccataat
    19321 cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatgt attttcctaa
    19381 cgtttgcgca ataaatttgt aactttcttt attaccaaaa cgggaaccgc aaaaaacagt
    19441 aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac acgtgttttt
    19501 ttaaaataaa agactagatt ttgtagtttt ttagtggtat ataggataga cgccactata
    19561 tatagtattt agaaaaaaat catttttcag ccattttata gataaaacaa aaataaataa
    19621 cgtgttttca tcgatatgaa atgacttact tatagaagat actgggattt cgtcctatat
    19681 gccaataacg tgtattattt taaacaacaa atacttgttt cttttattaa cgaaaaaatc
    19741 attttgttct cgtgttatgt ttcactgata aatgatgttt ctctaaataa aaaagccgac
    19801 gaactaaacg atcaaaattt gatcattttt gttcatcagc ttctcctaaa aatctttttg
    19861 ttaacgataa atagctggat ttgtaaaaat atcacttgaa tcgtcactgc tggaagaaaa
    19921 ttcagtgata accgcacctg ttgcgcctgc ctggaactag tgtttgatat tgggctcgaa
    19981 cgtgtactga tctctagcat gaagaataat ttcttttttt actgtataat aactttcttt
    20041 cctttttgga atagtacagg aaagtttttt tgtttccttt ccttcgacat aaggtatacc
    20101 agaccctttc gacaacgaaa ggttctcatt tttccttcat cgtcatttca aggagggtgc
    20161 cgatgctctg gacagtttcg atcgctccta aaccaaaatc tgtatattcg attctttcta
    20221 actctgattc tttcatacca ttcctgcctc aaggtacatc tattgtactt ttttcttgta
    20281 atccattctg cctctcctaa ctaattgtca aatcatccca agatatgtct tcttcctctt
    20341 tttcttcttg gatgatttct gcattcggat ctactggttt tttcaataat aaaatggtta
    20401 ttcctgtgac aaaagaacca gctaacaatg caccaatgaa tgcccatggt ctggtcatcg
    20461 tagcgactgc aaacattccc gatgccggaa tacggctatc tgcgccccaa aacatgctta
    20521 atccgccacc tactgtccca ccgactcccg ttgctatgac attgcgtgcc aagtcgttca
    20581 atacgatagg gaaacatccc tctgtgatct gacagatccc cataggaaaa gcggtcttca
    20641 atgtatcgat ttcttgacga ttgagtatat ttttacgaat gattttccct aagaaataag
    20701 cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat caatacagtg attggttcat
    20761 agattccttc tgaaaatcgt gccacacccc cttatccaga ataaatggta atccagaaac
    20821 agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc tatttttgtt
    20881 tggctgttct tattacttga tgcagggcag ctttttcaca acctcttttt ttatttatgt
    20941 ttcataatca atatggtaag gatgcagctc cgtatggtag accgcctccg aatattcaat
    21001 catttcccca atttgcgtag acgatttccg tatccgtttg atgccgatct gtgcgtctgt
    21061 tttcagaatt ccttgttctt cttctgttaa aaatactgcc gagaaatcat cagaaaatga
    21121 gtcaatagta tatccttttt tcgtcagcaa acgatacaaa gattctttgc caaattcttc
    21181 tctgctgaat tgcatcattt cttgtggaag ataatgaaca aaataaatat aaggctgatt
    21241 gttcaaataa tataagcgct taaaacaaac agcttgctca ccgaaaaagg aaaacaaagg
    21301 atcttccttc tttatatgga ccaattgcaa atccagcgtt tctttccgga cttcatatcg
    21361 ggagttctcc aagatttgcg taaagcttgc cgctttggat aatcgattgt aaggacgatt
    21421 gcttagaacc gtcgtcccac gaccactttt ctttgcgaca tattcatctg atgccagcag
    21481 ttcgattgcc tttcggacag tgattttgct gacttgaaac agttcttcta attcactttc
    21541 tgtcggcaac atcgaaccga ctggatattt tcctgagaaa atatcttctt taattttatc
    21601 tgcgacttcc aagtatagta cacgtgtcct tttcataacg tcaccctttg ctttactatt
    21661 actaaaagaa tagcacataa aaaataaaaa aagaacacaa atcacatgtg agaagtgtgt
    21721 tctttttcat tcagctgctt taattacaag tttacgccat ttgaggcaat tacttcttta
    21781 taccaatcaa aagatttttt ctttgatcga ttcagtgtcc catttccttc atcgtctaga
    21841 tccacataga taaagccgta acgtttactc atttctcctg ttgatgcact gacaagatcg
    21901 atacatcccc aaggcgtata gcccatcaaa tctacaccgt ctgctactgc attagccatc
    21961 gcttcgatat gacgacgtaa ataatcgatc cgataatcat caataatcgt gccatcctct
    22021 tccatatgat caatggctcc aagaccgttt tcaacaatga ataatggttt ctggtaacga
    22081 ttgtataatt cattcaatgc gattctcagc ccttctggat cgatctgcca gccccattca
    22141 cttgcctcaa gaaacggatt ttttactcct cccagcaagt tcccttctga agttgctgct
    22201 tgtggatcag tctcattgat tgccgtagac atataataac taaatccgat gtagtccact
    22261 ggatattctt ttagcaactg gaagtctttt tccgtttgct ctaatttttc cggatcgact
    22321 ccatattttt tatgggtacg cgctgtgtat gcagggtatt ctccacgtac ttggacatcc
    22381 gtgcagaaaa aattgaattc ttggttttgg atcattgttg ctgcctgatt gactggattg
    22441 gcatcgatgc tgtaagttgt tgcatagata atcatgcagc cgatttggat atccgaacgc
    22501 aattcgtggc cgatttttac ggctttgcta cttgcaacga attgattatg ccatgcttgg
    22561 aaaatatttt ggtactctcc tgctccattg ctttttacta atccttgact taaagctgga
    22621 aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat atttgacttt actgctgtat
    22681 ctctctaata ctgtctgagc aaatcgttca tagaagtcga tcagttttcg atttttccag
    22741 ccaccgtatt cttttgccaa atgaagaggc atttcgtaat gagaaatcgt gatcacaggt
    22801 tcaatatcat attttaagca ctcatcaatc agctgatcat aaaattctaa tcccgcttcg
    22861 ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc aggcaatcga aaaacgataa
    22921 catttgaatc ccatttccgc aaacaaggca agatcttctt tgaaacgatc gtaatgatcg
    22981 attcctcgat gattcgggta aatatatttt tcttgatcaa tcgtccagtc aaattcttca
    23041 ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag catctgcaac ggatagtcct
    23101 tttccgtctc gatcataagc cccttctaat tgatttggca gcagttgctc caccccataa
    23161 aaatttttct gaaaaacgtg attgcatcct gatttcctcc ttattttaca attgccagaa
    23221 catcttcgtt tgcaataatc tctttttggt ctaattcaag tatatcggca aatgcatcag
    23281 tattcgtaat gacaactgga gtgattacgc tatagccagc tgctctaatt gcttcgatgt
    23341 caaaacgaat caataaatct cctgctttga cgagatcccc ttgtttgact aagatctcaa
    23401 agccattccc atttaattct actgtatcca tcccgatatg aatcaaaatc tccgctccgt
    23461 cagtcgttgt gatccctaca gcatgtccag ttggaaaaat agtcgtgatc tctccgtctg
    23521 caggtgcaac tagttctcct acttctggat caatagcgat accttttccc atcacgccag
    23581 aagaaaaaac ttggtctggt acttcactca atggcagtac tttacccgtt aaaggacttt
    23641 ttaaggtatg ccgtgctgaa gtaatcggtt ctcctgcatt cgcatgtttg ttttctaatt
    23701 gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt acctacgaat gcaagaacaa
    23761 aagcaattcc tgtagctatt actgctactg tcacatttga ctctacacca tccaccgtat
    23821 taatgaaagt cggaatcgtc aataagctga ccaaactgct ggagaaagct tttactccgg
    23881 aaaatccgat aatagctcca ccaatacctc cagaaataca agcggcaatg aatggtttct
    23941 tcaaaggtaa tgtcacacca tacacagttg gttcagtgat tccaaataac gaagtcactg
    24001 tagaagaaat ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct acagctaaag
    24061 cagccccacc ttgtgccaag atggctggaa gaagcattgg catcaataca tcaaacccat
    24121 attgttcgat attcaaaaac atgatcggta cgaatcccca atgcatgccg aacatcacga
    24181 atacttgcca taaacttccc atgattagac ctgctacgat tggactaaat ccataaatcg
    24241 agttgaagag accgcctaaa gcattaccgg caaccgtccc gattggtcct aaagcaagaa
    24301 atgttaatgg aaccatcact actaaaacga caagcggaac taaaatcatt tgcagaaatt
    24361 gcggaatcac tttttttacg aatggctcta atttactttg aacccatacg gctagaatga
    24421 ttggtatgac agaagaagta taaccagatg gtgaaagaat cactgggatc ccaaagaaac
    24481 tgatagcttc tcccgcacca gcaactgctg tgatagctgg atacacaagt gccatagcaa
    24541 ttgctactgc taaaaactgg ttggctttga actttttagc tgctgtaaaa gcaagcatga
    24601 caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa tatttgataa gtaccgctat
    24661 cagctgtcag ccagcccatc accgttgcca atgataagaa tcctttcaat accccggcgg
    24721 ctgccatcgc ccctagaaaa ggtgtaaata tactagagat aatatcgatc agacgattca
    24781 aaatattttt ggactcttct tttccatcct ccgaaactgc aagaccttcc tcatccacga
    24841 tcgattgata aacatctgct acattactgc caatcactac ttgatactga ccgccacttt
    24901 gtacgacttg gatcacgtcc gggtcttctt ttaacttttg cgtatctgct aaagattcat
    24961 cttttaatct gaatcgcaac cgcgtagcac aatgtactaa acttttgaca ttcttttgtc
    25021 ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt atccattgtt ttttccttct
    25081 ttctttttta tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa acattcgtga
    25141 accttttttt tcaatccagg ttttgcctgc catacagtaa caatccttat gattggatgc
    25201 tttttttctc acttactact ctttgaatat gaatcgttaa ataaatctgc tctgcgatcc
    25261 ccatttcctg tttttgcgtt tgtcgaagat actcattgat ccgttcgaca caatgaaacg
    25321 cttttggata ctttccttga actaatgcgt agagaaattc atcttgctcg tcagatcctt
    25381 cccctttgag ataacgctgg gcaaaaaact gcagatgagt aatgatacgc tgataattca
    25441 aagattcttc atcgaacacg atgccgaaga atttactgat gatactcagt atatctctta
    25501 ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg ctattcacaa
    25561 aatgaaaggc aataaaaccg gcttcatcct cagaaagttc cacacccgtc ttttgtttca
    25621 cgaacaaaag agcctctttc gctgcttgat attctttcgg ataaaatttt tttgtttcaa
    25681 ataataatgg attaggcagt tggatatttt cttttgctcg tgtgatcgca taatgaagat
    25741 gatcggtcaa tgtcagataa atattaggcg aaaattcaac ttttagttta tcttcagcca
    25801 gttcgactac ttgtttgacc cattcgatct cctctgccgg aatatcagcc atcagctgct
    25861 ggaattggtt tttagcaaaa gaatctctta aaacaaattc tttttggacc gcttctggcg
    25921 aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt tcccatacaa acgacttctt
    25981 ccccgaatcc atttttcgaa atgacgacat tgttattcaa aattttatca attttcattt
    26041 tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc gcctactgtt tcttagctga
    26101 attctggttt tgcctgccgt acagtaacaa tccatttctt attcaaaatc atcataccat
    26161 cgaattttta tttatgcaag ccttttcata taaaaaataa aaaaactgga acacaagtct
    26221 atctagaaaa caaccaaatc agagatgcac ctctttgcgt ttctcttttt ttcagcaaat
    26281 tggtttttcc ttatcgattc atatcctttg gctgttttta atctgtaacg acttatgtcc
    26341 cagtatccta aaatgttttt gtctctagat tgaaagcaga tctcatatca tctgtcgcat
    26401 cttttatcga tgtaacactt tccagttctc cttgtacaac tagtcgcgtg acggcgtcca
    26461 tgtcgccaca agtaattagt gtgacaattt cttttccctc gacttcattc agtaattggg
    26521 tatctgtagg agcaactttt tctttaaaaa atgttttgta tgtatacaca ttttctaaat
    26581 ctgtcaaata gatcttggca cccatctcta aattttctag cggtgtaaac agcaattcag
    26641 ggttcgttgc acgatggctt gctaaggcat aatttccttt tcccatttct tgatctggag
    26701 ataatgttcc tgctccgtaa agtagtgacg tattatccaa gcctttgaaa accggtaaat
    26761 tgattgaaac tgaagggact gcgatactag caatcactgg tagatctgtt cctctcattt
    26821 gcgacctcat gaccccttct gtcgtcattg gttctaccgc atcaaagtca aaactgacat
    26881 ctttatcgtt attctttttc agatcttctt ttgttacatt cgctatagca tatttatctc
    26941 ctgtctctct gacaaaataa tccttgatct gttcattgaa aatcaaagct agtcctacta
    27001 ataacaagag gaacaagagg atattgacta gccatctttt tgcttttcct ttttttttca
    27061 ttttctctcc ctccagactc attttactgt ttttcttttg ctctttccaa cagccgaatg
    27121 acacatttct acaactctgt gtattttttt cgtacacctg cgcttttttc tacaggatat
    27181 ttcaaattat ttttgatcaa tttttcttca atgatctcat ctagatccag gtgaaggttg
    27241 tctgccatca tgtatgaata gatcaataca tctgccagtt cttctttgat tcgttctaat
    27301 tgtgtttctt ttacttcttc cggcgttttc cattgaaaaa gttctaacaa ttcagatgct
    27361 tccaaagaaa tagagatagc caaatctttt tcgttatgaa attgtcgcca attacgttca
    27421 tctctaaact ggttgacttt ttccatacta ttcatttgct tgccttcttc caatcacttc
    27481 ttacttgatt aatcatacca tttttttaca taaaaaatag atgatgcttt taaaaaaact
    27541 cctgttttct tttatttaaa ataaatgtca tagaaaatca ataatttagg tagccaccat
    27601 gtgtatgcag tagcgtgtct tcttcccttg aggctttttg tttttcgata cgttcttctt
    27661 cccaaacaga caacgctact agaatctctc ttaatgattt tccttcttcg gtgagcgagt
    27721 actctacttt aggcgggact tggttatagg cttttcgatg gatactttca tcttgttcta
    27781 actctcttaa ctgctgggtc agcatttttt ttgtgatcgt agggatttgc ttgcttctcc
    27841 acgcctaaaa taagcgatat tgctcttccc tttatctggt gtaagcaaag cagttagcat
    27901 tttgacgatc gtgggttccg cttttcactt cttatctact tagtatcatg aagtatactt
    27961 tcgtactaat actaagtagc ggaagtgtca actactttgt ttaactacat agcaaaagaa
    28021 acagaagggt gacaacggtc atcacgcttc tgtttctttt gaatatgctg tgttgttgtc
    28081 tctatctccg tgtagacttt cctcaaactt atcctttgac agtaagtcag taggctgatt
    28141 tgctctttct agatgccaga tacatttcta caaaaaaata gtgattctgt tttgaaaatt
    28201 cgatttttag ttctgcattt ccgtaaacat ccaacatttt tttcgcactg tatagaccta
    28261 acccgccatt ttttttagag gtttttcctc ttttgaccat ctccgatagc gtcaaattcg
    28321 ttggatttgc cttgttcgct atactaaaac gtgatccctc tttttcttcc agtagattga
    28381 tcgtaatcgg actctgatcg gctacacgat gatcgaatgc gttagaaata aaaatagata
    28441 aacagcgaat aaaaacaacc agatcaatcg ggattgtttt cacttcgaca ggaataacta
    28501 cttggatcgg gattttctcc gattctgctt tttctagaaa ttttaaaaag acacccctga
    28561 cagctgggga tacaatattt gttagctgtt gtatttgcgg tttttcaaaa atagctgcag
    28621 aatgatcttc taatatttct aaaaattcca acgtttcttt tttattttcc tgaagagtaa
    28681 tttttaatga aaacagtaga cttttataat catgtttgaa ttcctctaga tgatggtaat
    28741 attcttcatt tatttgatac agttgaagcg attcctgatg ttgtctttct tttacttgga
    28801 aactgatcaa cataatggaa aaaaacaaag aaaaaaaact gatgactatc gacaaaagag
    28861 atagaagcag ataattagat gagcggaagt gaacacttcc caataccaag taaatcacgc
    28921 cccagcttaa taaaatacaa atacctccaa aatagtgttc agttggataa gtaaaaagca
    28981 agttgagaaa tctcgtctgt ttgtctatac tttttactaa tagatagaaa ataaatgcga
    29041 taagtagtgc aaaaaaactt gctgttccaa gatgtctctt tcctaaataa tccatcagat
    29101 caaaaaccag aaaaaagctg agtccagtga tagcaaaaaa aagattactg atcaaacagc
    29161 tgataaacag gtttttcgta gtaaaataaa aaagaaacat caataataag ccaactaggt
    29221 aaggaaagct cactttaggg ataggaagta aaagtgtcac caccatcaaa gctatacaac
    29281 caaagctgtt ttttactagc agttgtttcg aaaagaataa atagatacct aaatctaaaa
    29341 tcaaagtaaa ggtcaaataa agtgtcactg ttgcgaccat cttattcctc cattgttttg
    29401 tttcaaaatg tacacatatt tttttaatag gagcctgcat atgaaaaagt attttatcat
    29461 agaagataac cagtttcaca gcgaatggat catcagagaa ttggtcaatc atcatatcga
    29521 gaatatcgtg cagctcactt ctatctctca aatcaatcag ttgatccagt tgggcactat
    29581 tgataatcac gatgttttct ttatcgatat cgacttgaag caatcactca caggtattga
    29641 tgtcgccgaa caaatacgta aaaaaaatgc acatagtaat attgtttttt tcaccgctta
    29701 tacgaatttt gcagtagaga tcatcaatcg gaaaatcctt ccgattggat atatcagaaa
    29761 agacaggaat ttatctgaac aaattcgtca agtgctagaa cagatcaatt ggaaagaaca
    29821 gcagatcgta aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta
    29881 tcctcgggaa attttatata ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa
    29941 aacaactaca gaagataaat tagtgaatac ttctttaaaa caattgaaga cagagttaga
    30001 aaagtatgct tactttttac ttttaaaatc ttactgtatc aacttatcgc aattgcagaa
    30061 aattgattct gctcattatg tgttagaatt tttcaacggt gactccttac tcgttggtcg
    30121 aaagatcttt gaaaagacaa aggaacgttt ccatgacttc caaaaaactg cttcgagttg
    30181 atgaataaga gctgagacgt aagcatagca gatttcaggg agtgggcctt gaatcggctc
    30241 cactccctga aatcgttgat atgattttac gcttcctctt ctgtaggagc atctgttaac
    30301 gaccatgtga atgttgaaac gtagttgtct acagcataat tggatacagg aacaaataat
    30361 tccactgatt gtctcccttg ttccacattc tctcctaatg aataaatcca tgtgccaatc
    30421 ccttcatttg ctcctgcagt tactagatct actgcctgat ctgctgggat ttcgattcct
    30481 tcagcgccag ttccagaaac ggtagatggc attccactgc tattagttgc gctatttcga
    30541 atcacggcgt ttttgaattt cacctttgaa ccagttagtt tattcgtatc agatgttgta
    30601 ccaataaatt ctgttcgttt gactgataat ttccaaccgg cataatttcc gcttttatcc
    30661 gttacttgta cgaaattctg tacttgtgtt tgtgctccag tactgtccgt tccgctttgt
    30721 aatgcagcat atactgtttt tgctgtacct aacttgattg ttccaaaatc aagatctgga
    30781 gcttggtcaa tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga
    30841 gtatcaggat catttggatc aacaggagga actggaacaa cgtcttcagg atttgcagca
    30901 tctggtttgg tcacagaaat cgttccttct gtagtggctg agctcttatt ctcagcaaaa
    30961 acactttgtg tacataataa tagacttcca gctagaacgg tacttaatgc aattgttttt
    31021 ttcatttctt tttccttctt tcttttaaaa taaataatcc agcaaataag ccaataagaa
    31081 tgactccaac aagtatcagc catcgccagt caaatgtttc tggctgattt tttaaatcaa
    31141 tatccttttc attcaattgg tccgctgcat catcagttat ttcaaaatcg gtatggagat
    31201 tccagcgttt ttctttccaa gcacccttca caaccgcttg ataatgtccc ggagctaatt
    31261 tttttccttc taatgggaca agtaaatcaa aatttgtatt gggtgctaca tctatttttt
    31321 ctttttttat tgaaaggtaa ggctcttttg tttctttttt gtagatttca accgatgtag
    31381 tcaagccgaa catataagaa gcatttggat tttgaagatt cgccttaatc acatttcttc
    31441 catcgctttg atccgcataa gcttttttta atttcaaatc tggggaagac tctttttcgc
    31501 tatttcaaat caagattgct ttcacttgag aaaactgatt gacgacacca actgtacttt
    31561 tgttagaaac gttctctttt ttttcttgaa acgtgatccc tcctgcgacc acaccagcca
    31621 gctctttttc tggcgctttc agtgaaagtg tctcagtgac cgcactttga ggaggaacag
    31681 ttatttctcc cactgttttt gctaagtttt tcatatcata acttaatgtt tcatcaaatt
    31741 tactttttga ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca
    31801 atgtctcaac tgttactgat ttatctgttc ggttaaagac cgtgtagggg atttcctgtg
    31861 ttttcccagg ctccatcctt acatttatat atgttttgtt cttatcaatc tgattagccg
    31921 gtaattcaaa atctaccgca aaattgaatt cggaggcatg gacaggtgtc tgccaaagga
    31981 tgaatccaag gagaaggatc ataagtaaag agcagttgtt tcgatgactt ttattccata
    32041 ttcgattcat caatcatcct ccttgatctt tattctgtcg gcgcatctgc taatgtccat
    32101 gttatctctg ctgagtaatt accagctatc tgattttctt tggttaagac aagtttaaac
    32161 gcccgaccgt tatttaaata ttctgaataa tctgcttgca cattcgttga attttgtcct
    32221 ctgacaattt ccagttcttc ttcattcact aattgatact cctctccttt cttgaaatat
    32281 aggacatcct gcatagaaaa tcctgcatca tttgtgaaag gctgagataa ttttatcgtt
    32341 aagatccacg gatttttcgt ttctcgctga tcataaatcc ataatttttc ctgtatatcc
    32401 atttggccaa atgcttcttg tgtcaaacca gttgctttga tctgaccaaa atcgagtttg
    32461 gaaggggcaa gaaatgagag accaccttct actacgatct tcgtagcagg tgcaaaaggc
    32521 ggggcatcat gataaacaac tggcgtatca cttggattgt gattcccaat ttcatcgttg
    32581 gtacttggct tgttctgcac acccttagca gtagcttctc ctgcttggtc gtttaataca
    32641 atttctaata catctcctgc aaaaagttga ttttctggga tagaaagaac aaattcccct
    32701 gtatcatcaa tagttgtttc tgacaaaaca gcttgatttt ttttcaatga aaccaaggct
    32761 cctgcttcac ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa
    32821 ttagcaggtt caggaggaat cacatcgact gtcgttttgg cagagtccag ttctgtcact
    32881 tcttggccac ctcttacttt tttagcagca acaacttgca cttgatcctc cgtttttaat
    32941 ctctcatcat tgggtacttg aacttcgaac aagccgcctt gttcgccttc tccccatggg
    33001 ttatatcctg ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt aatttctaca
    33061 gatgatttcg tcgtatctgc gtaagtaata cgtaaagtca cttctacttc gcctgtccaa
    33121 gcatctcttg gctttgcctc gacaccttcg ggaacatcga catgcccata gattttttta
    33181 tctgcattcg ttggcactct taaggattca atcatcggga gctgattatt ggcactgatt
    33241 cgattataat ttttcattcc ggcacctgtc tgagcaaatt catttgttaa acctgcacta
    33301 gggcttgata cgaaattgcc taagtcggtc ccattgatcg ataagtctgt catatcagaa
    33361 aaataatccg ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg
    33421 atttctgaat gggcgatctt taaagaagaa tctccatttc cttggaatac ccagccgcct
    33481 atgccattgg tctcgcttcc aatccgtttg ttttggaaat caaaataggt aggagattct
    33541 aagtccacat tcaacacatc cccactgatg atgccggtct tatcttcccc tgttttcccg
    33601 accatgacta aataagtttt ttcacctgcc gtaaaattta aatcaatatc tccagttgta
    33661 tctacagttg ctccggaatc tgcgcgtata tcgatatttg atttttctcc agtgactgta
    33721 aaatcagctg aacctggact ttctagaaaa ccagaagcgt cataaaattg gatcgcttga
    33781 tttccttgat tgagtctgtt cacgtaagat ttgccatcac ctgtatttct taccctcaat
    33841 gagcctccat tggttactgt cagcttttgc gtaccattct caaaacgaac tgcgctggaa
    33901 cgcccactct gcttatcgat gtcgataact gatccggctc cttctacgat caaagaaagg
    33961 ccttttccta caaaacgcat cccattgttc ccgggttgat cgtcatctcc agccattttg
    34021 accgagattt gtccaccact tgatacatgg atttgactag aatctccttg caagcgaaat
    34081 ccgcccgtag ttgctgtatt gatggtgatt ttcccaccat tcgatacagt gactttcgat
    34141 cctgaacctc tcatttggat gattccgcga ttgttgtcat tggtaatatt gatctttgta
    34201 tttaccgaaa attcgcttcc cgaaccactg acttcaaaca tgacattacc aggtagcgta
    34261 tcgactgcct ctccttcatc gctaacgcca tctgcccgat cataatcaag agagaccttt
    34321 gaaccgctta agatatggat tcctggcgct gtttttccac taccagtctc atctcctgaa
    34381 tctagaaaac cgtagaattt ccctagatac ggtccataaa gttgactacc attgatcaca
    34441 ttcaattgat agcttctaca aaaataggct ttacccggag cggcattcag tccttccttc
    34501 actggttgga catccaacac accggaaaca ccatctaatg tgacagttgc acgaggagcg
    34561 taaatcagtc ctaatttgga cccatcatac aaatcaagtg tcccggtaaa ttttatttct
    34621 cccgtaaaat gatatctgct tccccaaaaa tcggtcatat aagcaatgat aatggcattt
    34681 ccatcgatat ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa attctggaac
    34741 tcgatcgact gaggtatctc attgatataa atatggtgtg tgccgaaatt gtgagcgaat
    34801 ccaccaaaat caataacgat atctcgtttg atctctaatt ccctgtctaa agtcaaaggc
    34861 tgaatcagtc ggatcttagt tactgattcg tctgcgagaa gctgcgccag ttcttctgtt
    34921 gtggcagctt cgctgtaccc atccgctgaa acagctcttg ccagtgccgc ttcactgcta
    34981 tcagacgttt gttcagctac ttctgaggtc tcacttgtct tattttcttc ttgcctttta
    35041 ttagtagaag cctcactttg agattctgta gtttcagaag taatcgattc ttctgaaaaa
    35101 gtagtcttgt caaaactttg attctctgat tgcgttgatt cgtttgtttt ctctacatcg
    35161 gcaaaaacgg atgtcaccat caagagaggg aagacaaaca aaatcgacgc aatcagcttt
    35221 tttacacatt gattcattct ccaccatcct ctctgacgtt ttgacaattt tattttatcg
    35281 ttttaatcta tttccaattg tactttcata tttaaaatat gtatttttat ggaaaaattt
    35341 ttctattagg tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca
    35401 cagtccctta tcccgaacaa gcggtgttct cccgccaact cctcggtcac aacctccatt
    35461 ttaacaataa acctgtaaat gtgagggcaa tactttgatt gtcacaggta atttatcccc
    35521 ttcatctcca tcaacattcg tcgtcaattc ttcctcatca gctaatgaca caactaattg
    35581 ctcacaagta aggtaggcta gatttttcgt agattcatct actcctttta ataggtctgg
    35641 caatgtttta agcgtatcga tcaaagaaga atcttttaaa taaaccaaat ggagcttacc
    35701 atcatccact tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc
    35761 tatcagcaag gtactgctct caatttcttc cttctttcca tcaacttcta cgtgaaacga
    35821 gtaggattgt gtggaagcta actgtttgat tcctgacatg aaataagcta gttttccaaa
    35881 tttcgtcttc ttctccgcat ctacatcatt gattgattca gggatagagc caattgcgac
    35941 gacattcata aaatagtcgt catttatttt tccaatatcc aatggcttga ctgattcaat
    36001 cgaaaaatga ttgatcgctt cctcaggttc tagcggtatc cctaatgctc gagccaagtc
    36061 attcaccgta cccaatggga aaaatccgaa atttggccga tgttcttgtt cagcgattcc
    36121 actgattcct tcgttgactg ttccatctcc gcccatgaca aacacactat gatacccttc
    36181 cgtagccgct tcgcgagtaa agttttttgc atcccctgct ttttttgtat gcaagacgac
    36241 cacttcatca aacaccgatt ccaattttgc tatcgctaat tgctcaaatt cctttgcttg
    36301 ttcaccgcct gaactggggt ttaccactaa taaaactttt ttcatcactt tctttccctt
    36361 ctgattgatt tctttaatat aacgatagca taaagaaaaa aattaggctg tgatgaagta
    36421 ctgttcatga aacattcatt tattttttga caagataaaa catccatact atgaaactga
    36481 ggaaaattaa aacaatgata taggaaataa aagaaaatga taggcattgt atacctaata
    36541 aagtaaaaat cttaggtggc agataaaaac acaaaaatag caagagaatg taatagcgtt
    36601 tcttccactt tttgttatca gaccttatag cgatcagagg aataccaaat gcataactga
    36661 aaaactcttg atgtactgtt aatatgtcac ggccttgaat aataaatgcg acaatccccg
    36721 ctataattag tgtgcctaac accaaaacgg aaaattgatg aatatatttt atatacaaat
    36781 gatccattgt acttcctgcc tttttatttg tgagaaaaac aaatccgtat acgtgaataa
    36841 cttctagaaa taaaagaaat ttgcaaaacc actccattta tctacttcta cgtgcatttt
    36901 ttgatattgt tttctttatt taagattata cagaaaaata tctagggtcg ctggattcta
    36961 agtaaaaaaa atttttaaaa taaaaagaac taaatcaagt agtaattaaa ctactgttga
    37021 tttagttcta ataaaaagat ttagatgaac agttttattg atttaaatag ttcatatttt
    37081 ttatttatac cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa
    37141 cattttcaaa gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta
    37201 ctttattctg gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt
    37261 ttttgtattt atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat
    37321 attccgtagt ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt
    37381 tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt
    37441 tcacttttcc gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag
    37501 agaaatcgtc tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta
    37561 cttggataat ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt
    37621 catcggtcaa tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac
    37681 tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac
    37741 ccaattgttt ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc
    37801 catatttatc ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt
    37861 gcattttttt caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa
    37921 cggctgtttt ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg
    37981 caagtgctaa aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt
    38041 gctaaaaaca aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga
    38101 tacgtcctga atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt
    38161 caagtagcac atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct
    38221 tgcgttccgt atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt
    38281 cttccgctgc tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt
    38341 tgttgttttt attatgatat atttttatac aactaaaaat gaatatatta aaac
//
LOCUS       DACDLI010000018        36568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDLI010000018 DACDLI010000000
VERSION     DACDLI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.018"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV353_000998"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV353_000998"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9009266.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV353_000999"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV353_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9009267.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV353_001000"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV353_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9009268.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV353_001001"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV353_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9009269.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV353_001002"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV353_001002"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9009270.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV353_001003"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV353_001003"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9009271.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IV353_001004"
     CDS             complement(8053..8523)
                     /locus_tag="IV353_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9009272.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IV353_001005"
     CDS             complement(8569..8994)
                     /locus_tag="IV353_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9009273.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IV353_001006"
     CDS             complement(9061..9330)
                     /locus_tag="IV353_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9009274.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IV353_001007"
     CDS             complement(9341..10936)
                     /locus_tag="IV353_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9009275.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV353_001008"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV353_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9009276.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IV353_001009"
     CDS             complement(14489..15049)
                     /locus_tag="IV353_001009"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9009277.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IV353_001010"
     CDS             15402..16376
                     /locus_tag="IV353_001010"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9009278.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IV353_001011"
     CDS             16546..17946
                     /locus_tag="IV353_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9009279.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IV353_001012"
     CDS             complement(18024..18746)
                     /locus_tag="IV353_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9009280.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IV353_001013"
     CDS             complement(18825..19331)
                     /locus_tag="IV353_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9009281.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IV353_001014"
     CDS             19454..21253
                     /locus_tag="IV353_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9009282.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IV353_001015"
     CDS             complement(21321..22895)
                     /locus_tag="IV353_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009283.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV353_001016"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV353_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9009284.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV353_001017"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV353_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9009285.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IV353_001018"
     CDS             complement(24672..25310)
                     /locus_tag="IV353_001018"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9009286.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IV353_001019"
     CDS             complement(25311..25961)
                     /locus_tag="IV353_001019"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9009287.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV353_001020"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV353_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9009288.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV353_001021"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV353_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9009289.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IV353_001022"
     CDS             complement(29104..29844)
                     /locus_tag="IV353_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9009290.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV353_001023"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV353_001023"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9009291.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IV353_001024"
     CDS             complement(31215..32153)
                     /locus_tag="IV353_001024"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9009292.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV353_001025"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV353_001025"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9009293.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV353_001026"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV353_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9009294.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IV353_001027"
     CDS             complement(35313..35618)
                     /locus_tag="IV353_001027"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9009295.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV353_001028"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV353_001028"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9009296.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6706 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DACDLI010000019        36123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDLI010000019 DACDLI010000000
VERSION     DACDLI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.019"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(386..1969)
                     /locus_tag="IV353_001029"
     CDS             complement(386..1969)
                     /locus_tag="IV353_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9009297.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2547..2690)
                     /locus_tag="IV353_001030"
     CDS             complement(2547..2690)
                     /locus_tag="IV353_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9009298.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2913..3392)
                     /locus_tag="IV353_001031"
     CDS             complement(2913..3392)
                     /locus_tag="IV353_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9009299.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3465..3749)
                     /locus_tag="IV353_001032"
     CDS             complement(3465..3749)
                     /locus_tag="IV353_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009300.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3827..4021)
                     /gene="ssrS"
                     /locus_tag="IV353_001033"
     ncRNA           complement(3827..4021)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV353_001033"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4093..5379)
                     /locus_tag="IV353_001034"
     CDS             complement(4093..5379)
                     /locus_tag="IV353_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9009301.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5517..6188
                     /locus_tag="IV353_001035"
     CDS             5517..6188
                     /locus_tag="IV353_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9009302.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6154..6642
                     /locus_tag="IV353_001036"
     CDS             6154..6642
                     /locus_tag="IV353_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9009303.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6655..7602
                     /gene="prmA"
                     /locus_tag="IV353_001037"
     CDS             6655..7602
                     /gene="prmA"
                     /locus_tag="IV353_001037"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9009304.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7603..8367
                     /locus_tag="IV353_001038"
     CDS             7603..8367
                     /locus_tag="IV353_001038"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9009305.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8600..10813
                     /locus_tag="IV353_001039"
     CDS             8600..10813
                     /locus_tag="IV353_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9009306.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10824..11270
                     /locus_tag="IV353_001040"
     CDS             10824..11270
                     /locus_tag="IV353_001040"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9009307.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11441..12700
                     /locus_tag="IV353_001041"
     CDS             11441..12700
                     /locus_tag="IV353_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9009308.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12789..13736)
                     /locus_tag="IV353_001042"
     CDS             complement(12789..13736)
                     /locus_tag="IV353_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9009309.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            14130..15422
                     /locus_tag="IV353_001043"
     CDS             14130..15422
                     /locus_tag="IV353_001043"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9009310.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15437..17206
                     /gene="aspS"
                     /locus_tag="IV353_001044"
     CDS             15437..17206
                     /gene="aspS"
                     /locus_tag="IV353_001044"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9009311.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17315..18190
                     /locus_tag="IV353_001045"
     CDS             17315..18190
                     /locus_tag="IV353_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9009312.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18456..20339
                     /locus_tag="IV353_001046"
     CDS             18456..20339
                     /locus_tag="IV353_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9009313.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20414..20698)
                     /locus_tag="IV353_001047"
     CDS             complement(20414..20698)
                     /locus_tag="IV353_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9009314.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20688..21422)
                     /locus_tag="IV353_001048"
     CDS             complement(20688..21422)
                     /locus_tag="IV353_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9009315.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21551..22174
                     /locus_tag="IV353_001049"
     CDS             21551..22174
                     /locus_tag="IV353_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9009316.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22366..23487
                     /locus_tag="IV353_001050"
     CDS             22366..23487
                     /locus_tag="IV353_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9009317.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23494..26622
                     /locus_tag="IV353_001051"
     CDS             23494..26622
                     /locus_tag="IV353_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9009318.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26641..29835
                     /locus_tag="IV353_001052"
     CDS             26641..29835
                     /locus_tag="IV353_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9009319.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29981..30688)
                     /locus_tag="IV353_001053"
     CDS             complement(29981..30688)
                     /locus_tag="IV353_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9009320.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            31453..31851
                     /gene="spxA"
                     /locus_tag="IV353_001054"
     CDS             31453..31851
                     /gene="spxA"
                     /locus_tag="IV353_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9009321.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            32183..32836
                     /locus_tag="IV353_001055"
     CDS             32183..32836
                     /locus_tag="IV353_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9009322.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            33062..34060
                     /locus_tag="IV353_001056"
     CDS             33062..34060
                     /locus_tag="IV353_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9009323.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            34236..36044
                     /gene="pepF"
                     /locus_tag="IV353_001057"
     CDS             34236..36044
                     /gene="pepF"
                     /locus_tag="IV353_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9009324.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        11883 a         6399 c         7611 g        10230 t
ORIGIN      
        1 tgcatgtgtt ttttttgcgg agaagaccgc aaaatggtaa tttaacttca atttcccttc
       61 ttttttacag gatcttctta gggaattttg cttggaaaag tggtcaaaag ggaaaagact
      121 ggcattttat ttattgattt tcaggaaaat cccaatattt tattttcatt catctttata
      181 agggacatta catatgtaaa aagaaaaaac atttttagat cagcctcgtg ggctgtcgac
      241 tttattcttc gctttatgtt tagctagaaa accgatccga aacattcatt gatcgaataa
      301 taaaaacttg attttccacc tgtactttgc ctgaatcgtg aaagcacagg tatgaaaatc
      361 aagttttttg ttgcgaaaaa acgtcctagt caatcagttg gccgagcggc tggcggctga
      421 tctgctcctg atcaataacg agtgcttctt ggaagtgttg ctgcaaaaag tagcggtcat
      481 ggctgactgc taggagcgtt ccttgatagt cttgcagcat ttcttcaatt tcttctcgag
      541 catagctgtc gaggtgattg gttggttcat ccaaaatcag gacgttgatt ttttctaaaa
      601 agagtttcag caacgccaaa cggatttttt caccaccgga taaatctttg atccgtttac
      661 ccacatcgct ttgataaaag ccacttttgg ctaattgccg tcgagcgact tcttcgtttc
      721 cagtcaatgt gatggcgtaa gccagcaaac ggctatcggg atcggcaaac tgcagctgct
      781 gcggcaaata accgatttgc aagctactac ctaatttgat ggtgccaccg taaggctttt
      841 cctcaccgag taataatttg agtaaggttg attttcctgc accattgtcg ccgatgatgg
      901 ctaaatgatc tccacgatag actgcaaaac tgctgtcttt gaatagtact tgatcagcgt
      961 actgctggtg caagttttga atgatgaaaa cttctttgcc agattttcca ccattagaca
     1021 agctttgcag ccgatttttc gggggaggtg gcggtttgac gagtgtcagt ttcgccaggc
     1081 ggcgttccag ttctttggct tttttgaaga aggactcatt gtctccttca tgtgcccact
     1141 gacgaaaacg acgaatcatg actttcaacc gctggacttc tttttgctgc agttcgtaat
     1201 tttttgtcag ctcagccaag cgagcttctt tcaagactcg aaaacggctg tagtttccgg
     1261 gatactcaat caatgcacct tcttccagtt cccatgtttt tgtggtgact tgatcgagaa
     1321 attgccgatc atgagaaacg accagaaccg atttttttgt attctgtaaa tagttttcca
     1381 accagcgaat actgttaaga tctaaatgat tggtcggttc atcgagtaaa agtaaatcgg
     1441 catcggctgc taaaattttg gctaaagcga ctttaactcg ttcgcctccg ctaagttctg
     1501 ttaaccgtgc ttcctgacgg tgtggaatcc ctaagccttg caagatcgct gcaatccggt
     1561 cttctaatac gtaaccgcct agttcttcaa agcgatgctg cagctcaccg taacgggcca
     1621 gacacttctc taagtttgta tctggttctg tcatcaatgc ttcgtagcga ctcatttgct
     1681 gtttgaggtc ctgtatctca gcggcagaat ggagcaagta gtggttgacg gtcgcttcgg
     1741 aaactgtaag ttcttgttcg accgtcccga tctttaagcc ttttttgcga ctgataacac
     1801 cgctgtcgac accttctctg ccggtcaaga tttgtaaaaa ggttgatttg ccagttccgt
     1861 tgataccgac taaggcaatt ttttcaccag cttcaatttg acaggataaa tcctcaaata
     1921 atggattccc gccgattgtt tttttaatag aaattgcttg tatgatcatg atctctctcc
     1981 ttgagaccac tcaaaaaggc catggttatc catggcctgt gttatttaaa ctcagattat
     2041 catgagaaaa ataccttgag atggccattg attttacgtg aattcaactg ttttggacaa
     2101 acttgtcccg tcaacagcaa ctgtaaaacc aattgcatgg ctaaatgcga tatggtatcg
     2161 ttggtaattt tcattacttg ttattttagt catgcgcatc atagtatttt ccctccttcg
     2221 tgtagttgtc ttttccagtg tacctgaaag tgtcagaaag ttcaactagc gctgacattt
     2281 acttaatatt tatcgatgtg taattctgtc agagagcttt ttttatctga ctgagggtaa
     2341 tgtctcgtgg accacatcat tgtattctga gctaaaccag cgttcaggcg aaatcatatg
     2401 gtgtttgata aaaaactttg tctacatgac ttccttttgc catttgctta ataccgttgc
     2461 attaaacagc tcttctcttt ggaaatctca ccaacatcgc gttcaggagt atcgttgtgg
     2521 ttgcaatggc aagcggtagc ctttcatcaa ttcttgaatg atttaccgaa tgtagcactt
     2581 gccttctcgc ggtacccgaa cccgaaataa aattttagtt tggcgataaa atcggtatcc
     2641 gccatcgata cttcatattt tttcgtatca gattcatcgg aaacttccac attgacgaac
     2701 caataagctt tggcatgctt aggtcgtttt aaaccacacc agggcgacta atcgccctgg
     2761 tgttcacttt tttttatttt acttgaactc attgactgtt ttccctttat tttaaacatt
     2821 ataaagaccg atgagtgctt gccgcgtctt ttgtttttat cacggttttt tgtgcaaaaa
     2881 aacctgccga tagttggcag gtcgtctacg tgttaagctt tggtgtggtc tttagctggc
     2941 aattggtttt ctaaatcagt tcggacaaca agcacatcgc aagctgcgtt tctaataaca
     3001 tattcagaaa cagaaccaat aaataaacgt tctacagcat tcaatcctgt ggctcctaac
     3061 ataatcaaat ctacttgatt atcttctggg atttctctgg caataatttg ttttggtgag
     3121 ccatattcta cgacagaggt tacattattc aagccagctt tcttcgcatt gctttcataa
     3181 tctgccaatg tttgctttgc catttcagta gcttgttctg caagcattcc atcaaaggat
     3241 gaaacggttt ggaaagaacg tgtgtcaatg acatgtgcta atagaagttc tccgttattt
     3301 cggatagcta cattgaccgc ctttttaaaa gctaattcag cttcatcaga accatctacg
     3361 gcaaccatga tttttttgta ttgttgtaac ataagcattc actcctcaag tcaatcgtat
     3421 tgtactcttt tattgtagaa cactttaggg aaaaagagaa ttatttactg ctttggttca
     3481 gcaatttttt ttgcaaatcg gatactgaag gtggcagaaa caataattac gatcagtaaa
     3541 aagaccaagg caataaagcg ttgattgaaa aaagctgtta aaatacccat tacgcctaaa
     3601 acagcatatg cagctccgta aaattgtagg aatccttgat ttttatcatt tttgttgatc
     3661 aatacaaaaa agatatcctg tttattaaat aaataatagc taacgaataa taatactaaa
     3721 gtggcgataa tcattaaaat cctaatcatg aacaaatcct ccaaaataag caacaaatag
     3781 aaaaagcaat ttgccaagag caaattgctt tattctttaa aagatggtaa tctccgttaa
     3841 tgccgttgtt tgtcctgtag tattgatccc gcgaattcaa gcaggtgggt cccatggtcg
     3901 tagcttcgaa ctaccaaatg ataaggcatg ttaccagcag tgacacaaga cgggtcccaa
     3961 gatatcaata aaaatgttcg gtcaacacac aaagaggaca attcgaacat cacagatttc
     4021 ccacctctat agtagcatat gaatttaaaa agagcaatga gagagctcgc agaaacagaa
     4081 ccgttttctt gtctagtttt ttggaggatg ctttttccac tcttttatac ggtaatactg
     4141 ttgacccaga gcctgttcat atttccctgt atcttttggc tgataatact gagcattctt
     4201 cagtttatct ggtaaatatt gctgattgac ccaagcctga tcaaaatgat gaggatactg
     4261 ataacccacg ccacggttga gtgatttcgc acctttgtaa tggctatctc tcaaatgatc
     4321 aggaacatct ccagctttgc cctctcgaat atcagcaagt gctgcatcta atgccatgta
     4381 ggcagaattg gattttggcg acaagcaaag atcgacgacg acgtctgcta gtgggatacg
     4441 tgcctcaggc aatcccaatt tttcagcagc taatacagcg ttcactgtac gagccgcagc
     4501 agcaggatta cctaaaccta tatcttcata accgataacc atcagacgcc ggcaaatact
     4561 tgccagatca cctgcctcaa ctaaacgggc gagataatga agtgcagcat ccacatcact
     4621 tccacgaatc gatttttgaa aagccgaaat aacgtcataa tgagcatctc catttttgtc
     4681 atgggtaaga gcttttcttt ggacacattc ttcaatgatc gacagtgtaa gatgaatcct
     4741 tccttcttta tctgaaggag tcgatagtgt agccagttcc agcccattca atgcactccg
     4801 caaatcgccg ttcgttgcac gcgaaaggtg aagaagagca tcttcgtcta actggattgc
     4861 ttgttgtcca aggccgcgtt ctttatccct taaagcatgt tcgactgcca actggatgtc
     4921 ttgttctgtt aatggtttga cttcaaagat ctgtgtacgg ctgcggatcg ctggattgat
     4981 ggtaatataa ggattctcag tagtcgctcc gatcaagatg attcggccac tttctaaatg
     5041 aggaagcaga aaatcctgtt ttgttttatc taatcggtgt acttcatcca ataagaggat
     5101 cactgtacca ctcatttttg cttcttccgc tacaacttgt agatcctttt ttgaatctgt
     5161 agcggcgttt agcatgcgaa atgcataatt agtagagccg gcgatcgcac ttgcgatact
     5221 cgtttttcca gtaccaggag gtccatatag aatcatggaa gagagcatgc gagcatcgac
     5281 catgcggcgt atgatttttc cttccccgac tagatgctgc tgaccaacga cttcatcgat
     5341 cgttcgaggt ctcatgcgat aagccaatgg ctgttgcata ggattttcct ccttcttgtt
     5401 tttacgtccc ttaattatat catatttagc aagatgccta gaaagaacag ccttctgttg
     5461 ttttgttttc atagcttatc caactaatat tgtgtatgat aaagtaggtg agaaaaatga
     5521 cggataaaca aaagatagat gaattttttt ggaataaaac gacgccagaa gtggcacggg
     5581 atttattggg gatgtatttg gagcatgaca caccagaagg aagattagct ggatatattg
     5641 tagacgcgga agcataccta ggtccagaag acgaggccgc ccatagctat ggtttaagaa
     5701 gaacaccgcg tgtgcgggca atgtatgaaa aaccaggtac gatctacctt tacacgatgc
     5761 atactcatcg gattttgaac atcattactc agccagaagg gattcctcaa ggagtaatga
     5821 tacgtgcaat cgaacccgcc gcaatgattg atcaaatgag taaaaatcgt ggcggaaaaa
     5881 ctggtccaga tatcagcaac ggtccgggaa aattagtgga agcattagca attcctcaag
     5941 aactttatgg tcagtctata gcagatagtt cgttacgctt agtttttgag aaaaaaaaga
     6001 caccaaaaaa gatcaatgct ttgcctagaa tcggtatccc aaacaaaggt gtatggacag
     6061 aaaagccgtt gcgttttgtt gtgtcgggaa atccttatat ctctttacaa agaaaaaacc
     6121 aaatcgagaa agattggggt tggaggaaag aaaatgaaaa agaaggatct attaacatat
     6181 ttagatgaaa taattgaaaa acaagtgaca gactatgatg tagctttaga ttgggataca
     6241 aaaaatcata cgatcgagat cgttgttcgc ttgtttgcag aaaataaagc acaatttgag
     6301 attgatgatg cagaagggat cgtttcgcaa gaaccaatta tcgaatttga agacggcgta
     6361 cttcttttta atcctcagaa atctgttttt gatgaacgca attatctagc agtaatccct
     6421 tatgaaggaa aaaaagggtt ggcaaaatca gtagctgatt ctttagtgga atatctaaat
     6481 gaagtattag ctcaagggca aagcgatttg cttgattttt tggatgaaga cgatgacgaa
     6541 gctgtattcg agcttcactg ggataatcag caactagcag aattagtaga agtaaaacag
     6601 caaaatgaag cagatactta tttaccgtac ccaagttatt aaggagtgac agatatgaaa
     6661 tggaatgaag taaaagtaga aactgaaagt gaagcagtcg aagcagttgc taatattttg
     6721 atggaggcag gggcgagtgg tgtcgcgatt gaagattctt tagatgttga acattttcag
     6781 tcggatcaat atggtgagat tttagataaa aatgatttta catcgctgaa agaaggcgca
     6841 ctagtcatgg cttattttcc ggaaacgact ttcttaccgg aaatcttgcc ttttatcaaa
     6901 gatcgtatca cacaattacc tgagtttggt ctagctattg gaaaaaataa agtaacagtt
     6961 agtgaagtag aggaaagcaa ctgggcaaca gcctggaaga aatactatca tccggtgcgt
     7021 attacgcgaa tgctgacggt tgcgccaagc tgggaaaact accagacaga agatcctcta
     7081 gaaaaaatta tcacactaga tcctggtatg gcatttggca caggcactca tcccacgact
     7141 tccttgacat tgacagcact agaaacagtc ttgcgtggtg gtgaaacatt gctagatgtg
     7201 ggaactgggt cgggcgtgct aagtattgca gcaaaatatc ttggtgcaaa agatgtctat
     7261 gcctttgatt tagacgaagt agccgttcgt tcggcgaaag aaaatatgga tatgaatgaa
     7321 atagccaaag atgtccatgt atcagctaac gatcttttga caggaatcga gattgaaagt
     7381 gatgtaatcg ttgcgaacat tttagcagat atcatcttat tgatgatccc agatgcttgg
     7441 cgtttactga agcaaacagg cacgctgatc gtttcaggaa tcattgaaga gaagaagcaa
     7501 atggttctgg atgccatgtt agaacaaggg tttatcgtgg atcaaatttt ccagcaaaaa
     7561 gactggtatg caattatttt gaaaaaacca gaggtggaat agatgcagcg gtatttttta
     7621 aaagaatcat atgaatcaaa agactatttt gttttagaag gggaaaacta tcaccacatt
     7681 gtacgggtta tgaggatgga acccgaggat gaagtttttt tggcattcaa tgatcaaata
     7741 gccattgttg cagaaatcac ggagattact gaagaggctg tttatctgaa agaaattaaa
     7801 aaagaagaaa atcaaaagga actgccaagt tcgattacca tagcgagtgg ctatcctaaa
     7861 ggggaaaagc tggattggat cgttcagaaa tccactgaat taggagcgca agcttttctt
     7921 ggtttcccgg catcagcctc tgtcgttaaa tgggatgcaa aaaaacgtat gaaacgacaa
     7981 caacggcttg aaaagattgc tcaagaggca gcggaacaat cccatcggca gatcgtgcca
     8041 tcagtagagt tattggaaac gaaacaagat ttatttgagc ggctagatga ttatgacgtg
     8101 gtcttggttg cttatgagga atcagcaaag caaggcgaaa aatcaaatgc agccaaaatt
     8161 cttagtgaaa caaaaccaga tagtcgaatc ttagctgtct ttggaccaga aggtggatta
     8221 tcccgcgatg aagtcgatta ttttgtagaa cacaaaggga tactttgtgg attaggacca
     8281 agaatccttc gtacagaaac agcacctctt tatttattaa gtgctgctag ctatcaatgg
     8341 gaattaatga atccttcttt cagctaacgt tcttcattaa taaaagagta tcgacagaaa
     8401 gaattgaaaa aatagtcaac aatttgtata atagaaacaa ttatttatac aatggatacg
     8461 aaaaatcgtt tgaaaaagct tttaaaaagc gaacatttcc gtaaaaaaac gtataactgc
     8521 acggattttg ggacaattat caaacagata tttgtcccga ttcccgtttt accgaaaaaa
     8581 gaaaggggga tcgattcctg tgacaaaaga agaaatttta acgggacccg gtgtcataaa
     8641 gctcgttagt caatacatgg gtcccgaaca tgtagcattt gttgagaaag catgcgaata
     8701 cgcgactgct gcacatgacg gacagtttag aaaatcaggc gaaccctaca ttattcatcc
     8761 tatccaagtc gcaggtatat tagcagattt aaaaatggat ccccatacag tggctacagg
     8821 cttcttacat gatgttgttg aagatacaga aatcacttta gaagatctga gagaagaatt
     8881 tggcgatgac gttgctatgt tagtggacgg cgtaaccaaa ttagggaaaa taaaatataa
     8941 atcccacgaa gagcagctag cagaaaacca ccgaaagatg ctgctagcaa tggctcaaga
     9001 tttacgagtt atcatggtca aattagctga tagactacat aacatgcgta cgttgaagca
     9061 cctgcgagaa gataagcaaa gaaggatcgc tcaggaaact ttagaaatct atgcgcctct
     9121 tgcacatcgt ctagggatca gccggatcaa atgggaatta gaagatacag cacttcgtta
     9181 tctaaatcca aaacagtatt accgtatcgt ccacttgatg cagacgaaga gagaagaacg
     9241 tgaaaaatac gtaagtggta ctgttgaaga tattcgaata gcgacagaag agctggggat
     9301 ttttgcagaa atctatggac ggccaaaaca catttattca atctatcgta aaatgaaaga
     9361 tcagaaaaag cagttcaacg aaatttatga cttgctagct attcgagtga tcgtagattc
     9421 aatcaaggat tgttatgctg tattaggagc aatccacaca aaatggaaac caatgcccgg
     9481 cagatttaag gattatattg ccatgccaaa agctaatatg taccaatctt tgcatactac
     9541 cgtgatcggt ccggcaggaa atccggtaga aattcaaatc cgaacacagg aaatgcatga
     9601 aatcgctgaa ttcggggttg ctgcacactg ggcctataaa gaaggaaaaa atgaaaaagt
     9661 agaaccagat ggtatgacga aacaattaag ctggttccat gagatactcg aacttcaaga
     9721 cgaaagctat gatgcttctg aatttatgga aggcgtaaaa ggagatatct ttagtgataa
     9781 agtctacgtc ttcacaccaa aaggagacgt tactgagtta ccaaaaggat ccggaccatt
     9841 ggactttgca tacagtatcc atacagatat cggtaacaaa accactggtg caaaagtaaa
     9901 tggcaaaatg gtgcagcttg attacaaatt gaaaaacgga gatatcattg agatcatgac
     9961 ttctccaaat tcatttggcc caagtcgcga ctggttgaaa ttagttgcta ctagcaaagc
    10021 aagaaataag atcaaacgtt tcttcaaagc ccaagatcga gaagaaaatg tgatcaaagg
    10081 ccacgaatcc gtggtcaaat gtattacaga tctaggattt acgcctaaag atattttgac
    10141 gaagaacaaa ctgcaagaag cactcgatcg ttttaattat caaacagaag atgatctcta
    10201 tgcagctgta gggtatggag aagttagccc cttgacgatg gccaatcgtc tgactgaaaa
    10261 agaacgtaaa gaacaaaaaa tcgagcagca aaagcaagaa gcagaagaaa tcatgaatca
    10321 gccgaaaaaa gaacctgaca aaatgaaagt acgtcatgaa ggtggcgttg tcattcaagg
    10381 ggtagaaaac ttactaattc gtatcagtcg ctgctgtaat ccgattcctg gtgatgatat
    10441 cgttggttat atcactaaag gcagagggat atccattcat cgtcgagatt gtccgaatgt
    10501 tcagcctgac aaaccaaatg tagcagaacg tttgattgaa gtcgaatggg aagatacatc
    10561 gaatacacga aaagagtatg atgcagattt ggaaatttac ggctataatc gttcaggctt
    10621 attgaatgat gtacttcaaa cagtcaatgc gctaacgaaa aatctcaaca gcgttgaagc
    10681 acggacgaat aaagataaaa tggcgacgat ccatttgacg gttggtatcc agaatttatc
    10741 ccacctaaag agtatcgtgg ataaaatcaa agcagtacct gatgtctaca gtgtacgccg
    10801 gacgaatgga taggaggaca gatatgagag cagttgttca acgggtaagt aaagctgctg
    10861 tgtcagtcga tcagcaagaa attggaaaaa ttggctacgg cctacttgtt ttattaggca
    10921 ttcatgaaac cgatacgcaa aaagatgtgg actatctggt aaaaaaaatt gcacaaatgc
    10981 gtatttttga agatgaacaa ggaaaaatga atattagtgt agaagatgtg ggaggacaaa
    11041 ttttaagtat ctctcaattc acactgtttg cggatacgaa aaaaggaaat cgaccaagct
    11101 ttgtggaagc cgcacgacca gaaacagcta ttcctttata cgaatcattc aatgaaggaa
    11161 tcagaagtcg cggcatcaca gtcgctaccg gacaattcgg agcagatatg tcggtttctc
    11221 tcgtcaatga tggacctgta acgatcatta ttgattctaa aaacaaataa agtaatctgg
    11281 ttttcactag taaaaactat gaaacacgtc gcttaagtta gactgataaa ctctaactta
    11341 agtggctttt ttgtctgcaa aaagcgcttc ttttgtcagc agtcggaaaa aaagttaagc
    11401 ttaaattata caagttattt tttataggag gttgctgaag atgaaagaag taccagtagt
    11461 agatatcaaa atcacagatt taaaaaaatt gtatcaaaaa ggatacaaca tgttggagga
    11521 gttacgccat gaagcggacg ctcccgtcgt caaagcaaag attttcaata aagaagctat
    11581 cacgatctac ggatcatcag cagcaaaagt attttatgat ccaaggaatt tcaaacgaaa
    11641 aggtgctatg ccaaaattag tattgaagac actttttggt caaggtggag tacagacttt
    11701 ggatggagct gctcatcacc atcggaaaaa tatttttatg gatctaatga caccggaaag
    11761 aatggaagat tatcatcgta tcttagataa gaatctgaca caagcacttg aagcacagca
    11821 tggtcaattt gaactatttg atttaagtaa gatggttttc tttacatcta tttgcgagtg
    11881 ggcaggtatc aatttatcag cgatttctaa agatgaggtc gaaaaattag cagaatatca
    11941 aatctctatg atcagcggaa cgtttacttc tccgattgat cacataaaag gtgtcgaaaa
    12001 tcgcaaaaaa tcagaaaaat gggcgcaagg cttgattgaa gaagctcgac aaaatcctgt
    12061 ggctggaaaa gaaaatgtgg cattatatgc ttttgccaat gccacagatt tagatggtca
    12121 gcttttacct ctggaagtgg cagctgttga attattgaac atcatccgtc caacggttgc
    12181 tctaactgtg tgggcggcat tgatgggaca tgcacttttc agtagaccag atttgtatca
    12241 gcaattaaaa aatgactttt cgacgttaca agatccgttt attcaagaaa tgagacgcta
    12301 ttatcctttc ttcccaatgc tacctgcgat ttcgctgaaa gaagtagaag tagatggtta
    12361 tcgtatacca gaaggcagtt gggttatttt agatttatat ggaacagacc atgatgaacg
    12421 aacagttgaa gcgccagatt cctttatgat caaacggtat gtagggaaag caaaagatat
    12481 ttcctataaa gaagaatatg agatgattgc acaaggcggg ggaaattttc gacaaatgca
    12541 tcgctgtgca ggagaatgga tcactcttca tagtctacgt gtgttttccg atcagctagt
    12601 aaataagttt gagttctctg tacctgaaca agattggacg attccattca atcagtttcc
    12661 gacttatccg aatagccgag cgctacttta caagaattag attagcataa acttatcgaa
    12721 aaaaaggaag atcaaacagt gctgatcttc cttttttcat attactataa attgataagg
    12781 tcaattgatc aaaatccacg aaatgcttca tcaatggttt gataatcttc attggataaa
    12841 tgcaaatcta aagcttggac attgtcagat acttgctctg gacgcttagc tccaggtata
    12901 actacagaca ctcgtggatt cttcatgtac caagctaaaa caagttgcgc aggagtggct
    12961 tggtatcttt tagcaatggg gcgaagtaca tcgacagcgg ttaaaatttc tctgaaacgt
    13021 tctccttgga aatctggatt tctttttcga ggatctcctt caccaaattg tttttcctca
    13081 ccaagttcat attttcctgt cagcaaaccg gaagcaagag gaaaataggg aacaaaactg
    13141 attttgtttt tttcaagata agggaataat tctttttcag cctgccgatg gatcaaactg
    13201 tatttatctt ctactacatc gacatatcca tctgcattcg cttctttcaa ttgttctaac
    13261 gtgaagtttg acacaccgac tgctcggatt tttcctgctt cttttaattc gtgcaatgta
    13321 gcaactgatt cattttttgg tgtgctttca tccggaaagt ggatataaaa aatgtcgatg
    13381 taatcagttt gcagacgttt tagtgcatct tccaccgatt gtttgagaaa ctcaggagaa
    13441 ttatcaaatg ttcgcccttt atttggtaca tgagcagctt ttgtagcgat gacgacacgt
    13501 gagcgatcat attctttttc cttcagtacc tcgccaatca gttcttcgga acggccgttt
    13561 ccgtaagcat aagcggtatc gatcaaattg atcccgctat tcaaagctgt acgaactact
    13621 tgttttcctg tctcatcttc caaaccgtca aaaagattat gtcctccaac ggcatttgct
    13681 cctaatccaa gctgttctgc gtagacttgt gaatgtccga ttcttatttc tgccatagct
    13741 gattcctcct ttttgtctag tctaaaacaa aataccaaaa agctgaacta aaacgttttt
    13801 gtgaggggaa atagtcagat atctcttgac tttgagactg gtttttagta ggttataagt
    13861 aatatcgaat gtcaatgaaa gagcgagtaa gtttttgaaa ctttacaaga gaggatcatc
    13921 gaggctgaaa atgatctaaa gtttcattag cctaaagaca ctctggagat ggacgagtga
    13981 aaagccgtcc cgcgtacgag cgttattcgt aagaggaaag agaaatcttt caaaattagg
    14041 tggtaccgtg tatgaatttc attcaccctt gttcatgatg aacaagggtg atttttttat
    14101 ttacatagct aagaaaggga gagaaaacta tgagtttcca aagaccaaaa ggaacaaatg
    14161 atattttgcc aggcgaatca gaaaaatggc agtttgtcga agaaacagca cgtttattat
    14221 tcagagatta tcagtataat gaaatgcgta caccgatttt tgaacactat gaagtgatat
    14281 cccgaagtgt aggggataca acggatatcg tttcaaaaga gatgtatgat ttttatgaca
    14341 aaggggaacg ccatgtgacg cttcgaccag aaggtacagc accaatcgtt cgttcttttg
    14401 ttgagcataa attgttcggc ccagagtatg cgaaaccgtt caaaactttt tatatgggac
    14461 caatgtttcg ctacgaacgc cctcaaaaag gacggttacg tcaattccat caaatcggag
    14521 tagaagcatt tggatcagaa aaccctgcaa ctgatgtgga gagcatggtg atggcacttg
    14581 atttcttcaa acagttgggg atcacgcaaa tccggctagt catcaattca ttaggtgata
    14641 aagaaacgcg tcaagcctat cgtcaagcat tgatcgacta tcttttacct tttgaatcag
    14701 agctgagtga agactctaaa cgccgccttc atgaaaatcc attacgcgta ttggacagta
    14761 aagacaaacg agatcaaaaa tttgtggcag aagctccatc tatcttagat tatctaagcg
    14821 agcctgctca aaaacatttt gataccgttg tttccatgct ggatgcattg gctgttcctt
    14881 atgaaatcga cagtaatatg gttcgcggac tagattatta cacacatacg atttttgaaa
    14941 tcatgagtga cgcaccaaaa atgggggcac aagcaacgat ttgcgcaggc ggacgttatg
    15001 acaatctagt ggaagaacta ggtggtcctt caacacctgg cttcggattt gcaatgggta
    15061 tcgagcgatt actcttgact atggaggcag aggaagtagt tatccctgca tttaatgagt
    15121 tggatgctta cgtggtcgca ctcggagacg aaacaaatat cgaagcattg aaagtggtcc
    15181 aagcgattcg aaactttggt ttttcagctg accgcgactt catgaaccgt aaagcaaaag
    15241 cgcaatttaa gacagcagat aaagcgaatg ccaaattagt attggtgatc ggtgaagatg
    15301 agcttgctag tcaaacgatt aatgtcaaag caatggcaag ccgaaaagaa aaaagttttc
    15361 cattagaaga gatatatgaa cattttgatg aagtctatga tgaaatgact ttattcgagt
    15421 aggaggaaaa aaataaatgg cacaaagaac gatctattgc ggattagttt ccgaacaaca
    15481 attagaacaa acagttacat taaaaggatg ggtacaaaaa agacgagacc ttgggggagt
    15541 aatctttatc gacctacgtg atcgtgaagg aatcgttcaa atcgtcttta atcctgaaat
    15601 caacaaagaa gcatgggaaa tcgcggataa atgtcgcagt gaatacgtga tcgaagtgac
    15661 tggggaagtt agaagacgcg gagaaaatgc agtaaatcct aaaatggcca ctggtgaatt
    15721 cgaagtgatt gcaagtgaaa tcgtgatttt gaatcaagca aaaaccccgc catttcaaat
    15781 tgaagatgat caagcaatca gtgacgaaat acgtttgaaa tatcgttatt tggatctgcg
    15841 tcgtccaaaa atgactaaaa acatcaaatt aagaaatgcg acaacaaaag cgattcgtca
    15901 ttatttagat gataatgact ttctcgatat cgaaacacct tacctaggga aatctacacc
    15961 agaaggcgca agagactacc tagttccttc acgtgtccat gctggacatt tctatgcgtt
    16021 gccgcaatct cctcaattgt ttaaacaatt gctgatgaat gctggattcg accgttatta
    16081 tcaaatcgtt cgttgtttcc gcgatgaaga tttacgtgga gaccgtcagc ctgaatttac
    16141 gcaagtagat attgaaacaa cgttcttaac agcagaagaa atccaaactt acacagaggg
    16201 attgattgcg aaggtaatga aagaagttcg cggtatcgaa gtcacattgc catttcctcg
    16261 tatgacctat gatgaagcaa tggctcgcta cggcagcgac aaaccggata cacgttttga
    16321 tatggaattg atcgatctaa gcgatacagt caaagaagta gaattcaaag tcttccaaat
    16381 ggctcttgaa aacggcggtg tcgtgaaagc attgaatgct aaaggcgctg cagatcgcta
    16441 ctcacgaaaa gacatggatc agttaggtca atatgtagga caattcggag ctaaggggct
    16501 tgcatggctg aaagtagaag aagacggatt gaaaggtcca attgctaaat tcatgggaga
    16561 agcaacagaa gcaatcatta aagcaacaga tgcaaaacca ggtgacttgc tgatgtttgg
    16621 cgcagataaa agtgaaatcg tcgctgctgc attaggtgct atccggacac gtttaggaaa
    16681 agaattagga ctaattgatg aatcgaaatt caatttctta tgggtaactg attggccaca
    16741 atttgaattt tcagaagaag aaggtcgata tgtctctgct caccatccat tcacgatgcc
    16801 aaaagaagaa gatatcccgt tattggctga agaccctgca aaagtctatg cagaagctta
    16861 tgatatcgtc ttgaacggtt atgaactagg cggaggttct atccgtattc atactcgtga
    16921 gcttcaagaa aaagtactag aaactctagg ctttacaaaa gaacaaatgg aagaacaatt
    16981 tggtttcttg ttaacagctt tagattacgg attccctcca catggcggta ttgcattagg
    17041 attggaccgt tttgcaatgt tattagccgg agaagataat attcgtgaag tcattgcctt
    17101 tcctaaaaac ggaaaagcag cagatgtgat gagtgatgca ccaagtatgg tttcgccttt
    17161 acaattgttc gaattgaata ttgatgtaac gaatatagaa gaatagccga taaaaaggta
    17221 aaaaggattt gtcgaatacg cttcttttga caaccttgat ttaattgaaa aacactttat
    17281 aatgaagcca taacatataa agcggggctt ttccatgaaa aaaacattcg aaaattgggc
    17341 ttatcatgat tacacgacca agctttcggt atctatcgtt tatgcgttga tcgcatcagt
    17401 cgcattgaat ttcttttatc agccaggaca tatttattca agtggtatta ctggtctagc
    17461 tcagattttg acgactttgt caaaatcact gataggattc gaagtaccgg tatcaacaac
    17521 tttgtattta ctgaatatac ctttgtttgt attggcgtgg ttaaaaatcg ggaagaagtt
    17581 tacaattttc acttttatca cagtcacttt gacctccctt tgtatccata ttttaccgga
    17641 aacagtccta tctaccgatc caatcatctg tgcgttgttt gggggggctg tgatgggact
    17701 aggtattggg ctagcgttga gaaacggcct atcatcgggc ggtctagata tcgtgagtat
    17761 cacgattcgt aaaaaaacag gaaagtcgat tggttctatt tcgatctact tcaatgcatt
    17821 gatcgttttt gttgccggaa ttctatttgg ctggcaatat atgttttaca gtacgttgtc
    17881 tattttcgtc agtggaaaag taactgatgt cgtttatact aaacaaaaga agatgcaggt
    17941 aatgatcgta acgaaacatc cagaaaaagt agtaaatgaa atccaaaact gtatgcgtag
    18001 agggatcacg atcatcaatg aagcagaagg tgcttataag catgataagc agacagtatt
    18061 gatgacgatc gttacacgat acgaactgcc tctacttaga gaagcaatga aagcttctga
    18121 tccacaagct tttgtaagta ttgcagaaaa cgtccagata cttggtcgtt tctatgagga
    18181 tggcatttag atcgagtttg acaaaaattc atgaaatact gctgtagaca gacgttcgtt
    18241 tgtctgcagt ttttttctac catagtagaa tcagcaaaga aaaactgctt ttttatttga
    18301 aattttctta aagttgaaaa aaagcatgtt tattttttta aatttatggg atgatagaaa
    18361 aaaatagtag agaaaggaca cgattctact tgctattttc acttaatacc cgtatattac
    18421 gaatgaaaaa agtttatgaa aagttggaat attgaatgga aaaacaaaaa aggttaaaaa
    18481 acagccgcta catcactggg tttgatgggc tccgttcatt agctgtggtg ggtgttatct
    18541 tctatcacct tctgccaact agtatgaaag gcggctattt gggcgttccg atttttttcg
    18601 tcgtatctgg ttatttgatc acggatctgc tgcggcagga atgggaacaa aatggaaaaa
    18661 tcaatatttg gcaattttat gtcagaagaa tgaagagact ctatccggca cttgttttta
    18721 tgcttgtgac agcttcggct tatatcactt tgtttcagcg agggctgtta aacaatcttc
    18781 gaggagttgt ggtcagcagc ttactgtatg tcaacaactg gtggcaaatc aataacggtt
    18841 tatcttattt cgatcgattt gctaatgagt caccgtttac gcatatctgg tctttagcag
    18901 tagaaggtca aaactatttg gtttggccga tcttgtttgt attgttgatg gtatttgttc
    18961 gtaaaagaaa atggatcttt tatactgtgt taggtacatc cttcctctcc gccatcttga
    19021 tgatgatctt gtatacacca ggaggcgatc caacccgtgt ttattacggt accgacactc
    19081 gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa
    19141 agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat cgctggagga atctcgcttt
    19201 tggcactgat tctttgcttc ttctttttag atgatcatta caatttcatc tattacggag
    19261 gaatgctcct agtgagtctg ttatgcgtag tgctagttgc agtgacagca caccccggag
    19321 caagtttgaa tcgatggctg acaaatccag tatttacttg gattggcaaa cgaagctatg
    19381 gcatttatct gtatcagttt ccagttatga ttttttatga agcaaaggtg actgatattg
    19441 ccgatcatgt tttgttgcat actttagtgg aaatcacatt gattctaatc atcagtgaac
    19501 tttcttatcg atatgtggag cgtccgctag cacgttttga ttatggtcgt acattccaag
    19561 tgatgaaagg atggttcagc aaaccgatca tcagtaagag caaaccatgg gttgttccag
    19621 gcacattact tgtagcaatt gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa
    19681 cagccgatca aaaacaactg caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa
    19741 ccagaaacaa ttcagaaaac accactagtt cggttgacca agcgatcctt gataagtacg
    19801 agttgtccaa ggcacaaggg aataaggcac aaaaattaga actaacggca tttggtgatt
    19861 cagttatgtt ggatgctgca acggatctac aagaacttta tccaaaagta gtggtagatg
    19921 gagatgtcgg acgacaatta tatgccagtg taccgtacat tgaaaaatta aaggaacaag
    19981 atttgttgaa agacactgtt ctgatcggac taggaacgaa tggctcattc tcggaagctc
    20041 agtttgatag tgtgatggat gcaatcggtg atcgaaaagt atactgggtc aatgtccgag
    20101 taccgacgaa gcgctggcaa aatgacgtga atgcgatgct tgaacagatg acgaaaaaat
    20161 acgacaatat gacgctgatt gattggtaca gctacagtaa tgaccatagt gactggtttt
    20221 atgatgatca agtccatcca aatcctgaag ggatgacaca ttacattcac ttagtcagcg
    20281 aagccttgct aggaggaagt tcaagtacga acagcagtga gacaaacgtg acagaatgat
    20341 agctacaaaa aacttcaggc aaaaacattt gtttttgcct gaagtttttt tatatttacc
    20401 gtgtttttaa cattcatata gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac
    20461 gttctttttg ttttcgggtg agttttttga atgcagcttc tgcttttgtt gcttcgcttc
    20521 ttgaagaaaa tacttctgca tggatcatct taacaggtct tcttttttga agacgagtat
    20581 atttagcacc agtaccattg ttatgttcct ctaatcgtct agaaagatcg gttgtatatc
    20641 caccataaaa tgtctgatct ttacagaata atacgtaaaa ataatgttca tttgccatag
    20701 agcatctcac gaatctctgc agtgtattct ccatccatat tgtaagtgat caatggtgga
    20761 gcgactttga atccgtctaa tttgccttct ttgattcctt caactaacaa aatatttgcc
    20821 tcttttccag gttttgggta aacaaattgg atccttttag gtgcaattcg atagtcttcc
    20881 atgacctgaa tgatttctaa gaaccgatca ggacgatgga ccatagtaaa gcgtccattt
    20941 gtttttagta gcttacttga aatatcgatc acttcttcta acgtcgtatg aatctcatga
    21001 cgggcgattg ccaggtgttt attagggttc gttttatttg ttggcagccc tttgaaataa
    21061 ggtggattac agacgactaa gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc
    21121 agatcgatgg tgtggacagt catttgttca tttaaatggt taagcgcgat actacgtctt
    21181 gccatgtctg ctagacgagg ttgaagttcg atagcgtcga tagccgcctg ggttcgttta
    21241 cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt caacaatctt ccctttttta
    21301 ggtacacggg aaaagtgagc aagcagcaca gcatctaaag aaaaagaaaa cactttggaa
    21361 ctttggataa tcttgatatc ttctgcgtat aattggtcga ttcgttcgtc ttcgtgaagc
    21421 atggattccc tccaaaatga aaaaaatagt cgtctgtatt atactatttt tattaaaaag
    21481 aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac tatgaccata agaagggaaa
    21541 ggaagagact atgttttatc gatttatgcg cggggtagtt cgagtactat tagccttagt
    21601 taatggaaat gcccgctatg agaataaaga agttttaccg caagatgaga attacatact
    21661 agttgctccc catcgtactt ggtgggaccc attatatctg gcggtagctg cagcacccaa
    21721 ggaattcagc tttatggcaa aagaagaact gttcaaaaat ccagttctgc gatttatatt
    21781 aactcgatcc aatgcttttc cagttaaaag agaaaatcca ggaccaagcg tcattaaaac
    21841 acctgtaaag attttgaaat cgacaaattt gagtttgatc atgtttccca gtgggacaag
    21901 acatgctaca gaacttaaag gcggaatggc attgatttcc agaatgtcta aagcgaaaat
    21961 cgtgccagct gtatatcaag gtccgttgac acttggggat ctgttcaagc gtcagagagt
    22021 gacagtgcgt ttcggcgaac caatcgactt atcagatatc aagaaaacgg ataaagaagg
    22081 tatggcggag atcgaacgac gaacacaagc agcttttgac aaattggaca aagaagtgaa
    22141 ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc
    22201 ttttgaatct actgtcctgt tcagttcaga ctttttcaat gaaaacgagg gagagacgtc
    22261 aagtctgccc tctttttgcg ttccaatatg taaaatagaa gacaggtgtc atgtaatgga
    22321 tcaaagtgtt ataataatta gaaggacatt ttttgaggtg attgaatgcg ttttttacat
    22381 acagccgatt ggcatatcgg aaaaaaacta caaggttatg acttgattga agaccagcgt
    22441 catgtactat cttctatact agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt
    22501 gccggagact tatatgatag gagtgtccca gctgttgatg ccgtagaatt atacaatgaa
    22561 ttgatggtcg attggaattt aaaggaaaag atcccgattt ttgctatatc aggaaatcac
    22621 gatagcagta cgagactttc agcgggtacg ccatggttca gttattcgaa ctattttttg
    22681 cgaacacagc tagcagaagc atttgaacca gtcgagttta aaaacgtaca atttttcttt
    22741 cttccttact ttgaaccaat tgcagcgaga ttatattttg aagatgacaa tatccgaacg
    22801 atcaagcaag cggtagccaa ggtgatagat aaaatgcagg agctatttga ttcagaaaaa
    22861 aaacaagtat tgattagcca tttttttgta atgggaagtg taaaatctga ttcggaaacg
    22921 aaaatcgaag taggcgggtt ggattcgatc cctggtgaac tacttacacc gtttgattat
    22981 gtagcactag gacatttgca taatcaagct gctttgagac aggataatgc tcgctatagc
    23041 ggttccccat tgaaattctc tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt
    23101 gacctgacaa caggcctgga gttgaatttt agggagatcg aacccctgca tgatattgaa
    23161 gaaataacag cgagtttcaa agaattgctg gatcctgcct tttatcagtc cgtcaatcgg
    23221 gagaattaca tctatatcta tctcactgat agaacgatca tcccgaatat gatgaaccag
    23281 cttcggcaaa tatatcctcg tattttgggg attgaacggg caaacggtcg agcagaacaa
    23341 acaaagcgca aacgaaaagt actagaaatg aaagatcctc aaaaattagc agaagcattt
    23401 tttagtgaaa tgacggaaga gcagctaact gcaaaccaag aaaaatggct cggagaaacg
    23461 ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa
    23521 aattttggtc cttttatcaa tgagaccgta gatttttcac gtttgacaga agcaccgctt
    23581 tttttgatca gcgggaagac aggagctggc aaaacgacga tttttgatgg gatcaccttc
    23641 gcactttttg gtgaaacttc cggaagactt cgctctggaa aagagatgcg ttctttgttt
    23701 gctacaccag acgaagaaac aagcgtgact ttttcttttg aacatcagca gatgagctat
    23761 gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa
    23821 caggcagcca aagtaacgtt gaccattttt gatgggaaag gaaaagaaat aaaacaaatc
    23881 acaaaacgta cagaagcaga tcagttgatc agagaactga tgaatctcga tgcgaaacag
    23941 ttttctcaaa tcgttttact accccaagga gaattccgta attttttggt ttcatcaagc
    24001 agtgataaag agactgtttt acgtcattta ttcggtactc aatttttcca acagttcaat
    24061 gagcgattaa aagaaaaagc aaaacaacag caacaaaaat tggatcattt agagcaagag
    24121 ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag aggaaccagc agcagggttt
    24181 gaagccgttt tagcccaatg gacaaaccat caggcaatac tcgaaaaaca aatcacggaa
    24241 gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc aactagaaaa ttcctactat
    24301 cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga tattaataaa caagcaaaag
    24361 gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac gatggattgg gtattatgaa
    24421 tttacgcaac gattagtcga accaatccag cgcctacaag aaataaaagt tgaaaaaata
    24481 gaactgtcac aaaaaagtag tgagcaaaca aagaaactag cgatgactac gcagaattat
    24541 gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac aaacaaaaat caaagaagct
    24601 gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg tcgaatcctt acgtagtaaa
    24661 aaacaagacg cagaacaatt aacaatttca ttggataaag aagaattgaa tgccgagaag
    24721 ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa ttcttcaaga aaatttactg
    24781 gatcaggaaa aactgcaaga agatcgtctt gcttttggac agttaaaata tctagatgaa
    24841 aagcagcaag aaatagaaaa acaaaaagca aaagacactg ggtcgctcgc acagataaga
    24901 gaagaactca aaaaagcaac tgaagaaata acaaaaaata gtcagctatt gaaagaacag
    24961 acagaacaag cagaacagct gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag
    25021 ctttttttgc gtccagggga gccatgtctt gtttgtggat caacggaaca ccccatacaa
    25081 caattaaaac acgaacagcc aaataaggaa gatatcttaa aagtagaaca acaactggca
    25141 gaagctgagt ctgctgtaga aagaactcag caaatcattg ccaaaagtga agcacatgaa
    25201 aataatcttc aacaacaatt atcagattta gaacaaaaaa tcatggcaac gaatatacaa
    25261 ttggctgaac aagaagaaca cttgcaacag gctttgaaaa aggcatttcc tgagataaca
    25321 gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat ctttagatag acgaaaaagc
    25381 aagatggcca atgcaagaat agagctggaa gaaataagac aacaagcaga aaagctcgaa
    25441 caacaattag ctgtctacaa acagcagcag acagaaaaaa ataatcagct ccagcaactg
    25501 caaggagaaa tcaaaagttt agaacaacaa ttgcaagata cggatagtaa ctgtttagaa
    25561 gaggagatac gacgacttga aaaacagtta gaagaagact cgaatcaact agctgttgag
    25621 cagaaagcag gagtgcaatt gttgcaagat cttcatacgc ttcggacaca gcttgatctt
    25681 caacatgaac aaatagaaaa aatagatgaa aaaatagaaa aggaacaaga aagaatagct
    25741 aaaaagttag aaagccagtc gtatttttca acaattgaag aaatctgtgc atttgcacaa
    25801 cagcaagcac aatataaaga aaaccaacgt acagttactt cttatcatga agagcaatta
    25861 gtgacagctg accggctgcg tcaattagaa aatatccagc taactgagac attcccagat
    25921 acagaagaga tcagccaaca gttagaagaa ataagacgta tgatcgaaat aaagcaagaa
    25981 aagatatacg gtttacaaga acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt
    26041 tcgatttatc acacaagcca gacgaaatta gatgagctta cgcaattaca gcagctttcg
    26101 caaacattca acggagaaaa tcctaagaag actagtttgg aacggtatgt gcttcaggtt
    26161 tatcttcaag aagtgttaca agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt
    26221 tatcaatttg agctagcgga taccattggc agttatcgag ggaaaaccgg attagaaatc
    26281 aatatctatg atgatcaggc aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag
    26341 agttttattg cagcactttc tttagcatta gctcttgccg atgtgatcca gacacaagct
    26401 ggtggtgtag caatcgatgc attgtttatt gatgaaggat ttgggtcatt agatgaagaa
    26461 gctttggaaa tggcaatgga agctttagaa acgatcgaaa gtgaaggaag aatgatcggc
    26521 atcattagtc atgtaagaga attgaaagaa cgagtattac aacaaataag aattgataca
    26581 gaaggtagcg gacaaagcaa aatacgctat caacttggat aaaaagggag attggtaact
    26641 atgaaatgga gtattccaga aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc
    26701 cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc
    26761 agtgaattgt atatggttga tttggatggt acagcaaaag agatagatta ctgccaatgt
    26821 ccctattggg aggaacataa atattgtaag catacagttg ctgtggaact ttatttacgg
    26881 tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca
    26941 acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct
    27001 caagcggtgc aacctttgat tattgaatac caagtagaaa cgattgagac taatcagtat
    27061 cacccagagc tttctatttt ggcactttac ttgcgtatcg gttcattggc atcaccgaaa
    27121 aaaacatata tcgtaaaaaa catctatgat tttttacaag cttacacaga aaaagaaact
    27181 tacactgtta ataaacagta ccagtttcgt ctagataaac gggcgtttca aaaagaagat
    27241 caaaaaatac tcacgcatct tgcaggaagt gctcagacgc atcaattatt aggtacgaac
    27301 ggcttacaag tcaaaggaaa actagataaa agatatctgc ttctcccagt agaacaagcc
    27361 cgcttcctgt tagagcagat gaatcagact acacgtttgc atctgcagat aaatgaacaa
    27421 aacgatcaag gaatacaatt ttccgatcaa aacaaacctt tatcattcaa agtggaaaaa
    27481 tttggagaag attatttgct gcaagcattg aatgattttg attttttctt tatgcattat
    27541 cagtggggag ttatccaagg gacattttat tctctgacga aatatcaaca aacggtttat
    27601 caaacttgga aacaattgat tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa
    27661 gaattaccgt ctttgtttaa agagattatc cctctgctgt cagagatcgg cgacgtagct
    27721 attgcccctg cagtagaaga ggaagtagca gatatccccg tcgaatttat tttctttttc
    27781 cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg tctatggcga agtcatctat
    27841 tcgactgacc cgaagcatga ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca
    27901 gtagaagaga aacgagtact tgatctattc agaatgtatc gctaccagaa aaatgaaact
    27961 ggttatgaac gtccattgcc aagcggggaa gaactatatg cctttttccg cacagaacta
    28021 tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa aattgcgaga gttgtattta
    28081 gatgcgcata aacatcaacc gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt
    28141 cgattcgatg tgacagggat agaggagcaa gaaatcgatc atgtcctgca aagcttgctg
    28201 agaaatgatg ctttttatac attagaaaat ggtcaggttc tttcttttga ttcagaagaa
    28261 tttcaacaaa caagccaagt gttgcaacaa ttacgggaat cgatacgtac agaagaagga
    28321 acgatccatg tacctaaaaa tcaaggattg atcattcaaa atcaattgga aaaaagcaat
    28381 gctactttta gtgaatcgtt ccaaacgatg gtacaagatc tcattcatcc agaaagatac
    28441 caggcacagc ttcccaaagg attgaatgca acgatgagag attatcaaaa gcaaggtttt
    28501 cgatggttga agatgttagg tcattatcag tttggcggta ttttagccga tgaaatggga
    28561 cttgggaaaa cactacagac aattgccttt ttactttcgg aaaaagaaga acggaaaagc
    28621 ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca actggcaggc agaggtgaga
    28681 aaattcgccc cttctttaag tatccaagta atcaatggga ataaaaaaga acgtgaagaa
    28741 ttactggcta aagacacaga tatccgagtg acttcttatg ccagcttaag acaggatcta
    28801 gctaattatc agtcgcaaaa aattgattat ttgattcttg atgaggcaca aatggtcaaa
    28861 aatagcagta cgaaaactgc acaagcattg agagaattag ctgtacctca gcggtttgca
    28921 ctgagtggga caccaatcga gaataattta gaagaattgt ggtcattgtt tgcaacgatt
    28981 atgccaggat ttttcccgac taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg
    29041 caaatgattc gaccgtttat tttacggcga gataaacaaa ctgtgttgaa agacttgccg
    29101 gaaaaaacag agatgaatct atattctgca cttacagaag aacaaaaaac tgtttattta
    29161 gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg attctgaagc attcaagaaa
    29221 aaccggatcg ggatacttgc tggtctgaca agactgcgac aaatttgctg cgatcctcgt
    29281 ctgtttattg aagattacca aggcggttcc ggcaagctcg aacaagtaaa agatctactg
    29341 gtagctgcca aagagaacaa acgaagagtt ttgctgtttt ctcaatttac agggatgctg
    29401 actatcttgc aagaagagtt agccgagcta gggatctcga cattttattt aagaggcagt
    29461 acgaaaccac aagatcggtt aagtatggta gacgcattta atgctggcga aaaagatgtt
    29521 tttctgattt ccttaaaggc tgggggaact ggattgaatc tcacgggagc agatacggtc
    29581 atcttgtatg acctttggtg gaatcctgcc attgaagaac aagctgctgg acgtgcacat
    29641 cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga tctctgaagg aacgattgag
    29701 gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt
    29761 aatgaagaac agttgacgaa actgactgaa gaagacatcc gcatgatttt aagtgtagga
    29821 gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa
    29881 agaagcgatt gtttatttga tttaaaaaat agcaaggagg ctgggattcc attttatccc
    29941 agcttccttg ctatcatttt tttatttcta tttttaaagg ttatacaaag attgtcttca
    30001 ttgtctctaa cagctgatca aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa
    30061 ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt agcacttttt gcacctagaa
    30121 catcattgtc gtaattatcg ccaacgtata aggtctcttc tggaagcata tgaaattctt
    30181 tctcagctag ctgaaaaatc tctttttctg gtttttggaa acctgttgct tgagaaatga
    30241 gcatattgcg ggaaggaatc caattgttca attgaagttg cttcagtttt tttgtctgat
    30301 ggtcagtcgg cccatttgtg ataattccta atgggacatt cttttccttc aagaaatcga
    30361 gggttttttt gacctcctca tgcaagcaga tattatcaag ttcttcctcg taaatttttt
    30421 gaaataagag gccatcttct tccgtaatat gagggtagtc taaatctttt agtgattggc
    30481 tgatccgatg tgctcgcata tattctagtg tccaatctcc tgccattact tttgggaagt
    30541 tttcatcact atgatggcgg aaccggatat atagtggatg catatctgca tctgaaacaa
    30601 gcggaacgac tcttttgacc gcgtttcgaa atggctgctg ctgatcatac atcgtatcat
    30661 caacatcaaa cacaactgat ttaatcaaaa ttttacacgc ctttcggttt tcattgattt
    30721 tttcacaaat ccttgactat tgtaacagaa aaaattgtct aaaaaagctg ataaataaag
    30781 gtttttctta ctatatttct atttccttga cagaataagg tttttatatt tcagtttttt
    30841 tcactaaaaa tcgtgaaaca cgtcaataag tttgatgaac aaattaatca ttatggtaga
    30901 agtcgattga tttattttaa tgcaatctct gtggaatttt cgaaagattt atttttttaa
    30961 tacagtctca atataactat tcaaacataa aataacgtta tttgatttat ttgtttatag
    31021 taaattttat ttagtaatca ttgctttaaa atactacgct tatataacag agaaaataag
    31081 cgttataata aagaacagta ttatttaaaa tgacagaaaa cattactatt aaagaaataa
    31141 aaaacataaa aaatcttaag aaatcggttt catttagctt ttgagataaa ataatagtac
    31201 agttttatac gctgtttcat atgtttagaa aatttgaatt agatttgaaa actctaattt
    31261 ttaaatgata aaaaaagcga gaatgctggt tttactgtat attaacctta gataactttc
    31321 atttttttaa aaaaattatg aaaaaaaggt agctatttaa aagttgatta tgtataatag
    31381 aaacagatgg gagattatta tgatcttctg ttacaaaaaa gtgattctta tcacgaaaag
    31441 gagtggtgca cgttgttaac attatatact tccccaagtt gtacttcctg cagaaaggct
    31501 cgtgcgtggt tgcaagaaca tcagattcca tttgttgaac gtaatatttt ttcagaacca
    31561 ttgaacagtt ctgaattaaa agcgatttta caaatgacag aagacggaac agaagaaatc
    31621 atctcgacac gttccaaagt attccaaaaa ctgaacatgg atttagatga tctgccgtta
    31681 caagaattat tggaacttgt gcaaaacaat cctggattgc ttcgccgtcc aatcatgatt
    31741 gatgataaac gtctgcaagt tggttttaat gaagatgaaa ttcgtcggtt cctaccgcgc
    31801 gatgtacgac aattagaatt acgtcaagca caattgatgg ctggattata gaaggataat
    31861 acgaaacttg gaaaagacta ttacaaagat agtttttcca agtttttttg tttttacaaa
    31921 aagtattctt gattagatgt ataaaaaggt agtagaagat gatgattgca cgaaaaatag
    31981 agagggaaaa ttagctgaaa agtcggctat atagtattcg tctgccaaaa atgttatagt
    32041 taaataagat agcttgaaag gttgaaaaat ccgctggttt tattaaattc gcttaacagc
    32101 gagatttttt ttctttacat ttaagctgat ttcgctacaa tgaatctata ttaaacattg
    32161 tggcaaagtg aggtgtagca aaatggaaat ggaacatatt aatgaaaata cgatccgtgt
    32221 actaattggt catgaagatt tagaggaacg gggcgtttca tttttggatt tacttggcaa
    32281 tcacaaagaa atcgaaaatt tcttttatag tattttggaa gaagttgata tcgaagagga
    32341 gttccgagga agcgaagctg tcactttcca agttttgcca aaaggagacg gattagaact
    32401 gtttatcagt aaaaatcttc cggatgaaga aatttctcaa tcagaagatt ctactgttga
    32461 ttcagaagat gtgagtgact ttcttcgtca gcagattatt ggcagcgatc cggaggaaga
    32521 attatctgga agcgccctag aagaacagga aagacaagca acttttgctt ttgaagattt
    32581 tgaacaagtg atccaacttg caacagatgt cgaactagag tctgcttgga ctgatttgta
    32641 tttattcgaa gagcgctatt acctagtcgt tcacttctgg atggaaaatc tcaaccaagc
    32701 ggatgtagag aatcagattg ctcgaatttt ggagtttgct gaaaagagcg atcgtacacc
    32761 agaaacattg aatgaatatg gacaatgtct gatggcgcgt aatgctatcg agcgaacgaa
    32821 attttatttt gtataaatca gcattatagt aagagactgt gacaaaaatt gtgtcgcagt
    32881 ctttttttcg tataaatgtg ttcaaaagct tgcatctgcg gtaacaagcc caaccaaacg
    32941 aaaaatattg ggaactattt ttgtttagct gttcttatta cttgatgcag aatagctttt
    33001 tcacgccctc ttttttcgtg taaataaatt cttagacgta aataagttca gagggagaaa
    33061 aatgctgctt gcaatgaatg aatataataa gtgctgtttt gctaaacatg cagaaaaagg
    33121 aaagaaatat tattgtcgtg gctgtcgaaa aagagtgatt ttgaaaaagg gaaagaaaaa
    33181 atgtgctcat tttgcccatc aaaaatcaga taactgttcg gtctttagtg aaagagaatc
    33241 aaaagagcac ttacagttaa aggaatgctt catggattgg ctgggacagt cagtagaacc
    33301 agtatttttg gaagcctatt tgccgaggtt gcggcaaagg ccggatattt tgttggcgaa
    33361 tctagcaata gaaatccaat gttctagact gtctcaccag agatttattg aaagaaccca
    33421 aaattatttg aataacagtt accaagtatg gtggatactt ggtaattctt ttcttggaca
    33481 atcgcaattc tcattgatag aaaaaagttg ctgctactat aatagaaaaa gaggagtgca
    33541 ttgctggaaa gctgatctca agaaacaaaa gctttatttg tatcatcata ttacagagac
    33601 tgtatcaggg catatttcct tttgcagtag ctgctggact ttttctagta gggaccttaa
    33661 agaaatattc actagtaacg aaattaaaat gaaccaaatg aaaaaaatag aaaaagtgag
    33721 tgaagatggg aagaaatggc tgacacgcca acttattcac aagcaaaaga atacagtatc
    33781 catacaagaa caatgctact tgcgtcataa acatcttttg catctatcat cgtggatcta
    33841 tcaaaaaagt cggtttttct tttatttaca agaacaggtt tttctttatc gaatgttgta
    33901 cgaagaagcc ttggaaaacc agaaagttcc tgatttcaac tcatggcttt gccaaataaa
    33961 agaacataaa agggaatggc tttttcctat gattgatgaa gagatggtct atcagcagtt
    34021 ttttaacgaa tgcatacact tgtcttcttt gaaaaaatag taagtataaa aataggctag
    34081 gaatcggttt tctatgtaaa acaagattta gttatgctaa aattgatgga agcagacaca
    34141 tgaatgagta gaaaagttag atgctgtgac atgtggatgt tagactatga tctattttga
    34201 tagatagagc taagggaaga aaaggagtgg atttcatgga agtaaagcag ttgccaaaac
    34261 gagaagaatt gcctgaaaat ttaacttggg acttgaccaa gatcttttca agcgaccaag
    34321 agtttgatga gaaatatttg gaattatcag aagagttaaa acaatctgaa aaacacaaag
    34381 gaacacttga tcaaggcgct tctcaatttt taaatgcgat tgaattcgta ttgagggttt
    34441 atcgccaaac tgaagtcatt tatgtatatg cgcaccttaa aaacgatcaa gacactggaa
    34501 atacagatta ccaagcgctt tatgcaagag caagcagtct gttttcgaaa gttagtgaag
    34561 ccgtttcctg gtttgaacca gaaatattgc aattgtcaga tgaccagatt tggcaatatt
    34621 tcaaagaaga accaaaattg gaagtctatc gccattatat ccagcaaata gtagataatc
    34681 gagcccatgt cttatctgct gagcaggaat ctcttcttgc tggagcaggt gaaatctttg
    34741 atgcttcaag tgatacattt gctgttttga ataatgcaga tctagttttt ccaacgattg
    34801 aaggagaaaa tggtgaaata gtccaattat ctcatggcgt gtatggtcag ttgctagaaa
    34861 gcacggatcg aagggtgcgc gaagcagcat ttaaggggtt gtacagtgtt tacgaacaat
    34921 ttagaaatac atttgcttct actttaggca cacatataaa aggacataat tttaaagcga
    34981 aagtccgtaa ttacagctct gccagagaag cgtctttgag caataatcat attcctgaaa
    35041 gtgtatacga tactttggta gacgtggtaa acaagcattt gcctttgtta catcgataca
    35101 tggaattacg gaaacgttta ttagaagtgg aaaaactgca catgtatgat ctttatacac
    35161 cggtcttagg ggaagctcca attaccttta cgtacgaaga agcaaaagaa aaagctttag
    35221 aagcactgaa accaatgggt gaagaataca tggccatcgt agaaaaagca ttctctgaac
    35281 gttggatcga tgttgtcgaa aataaaggga aacgaagcgg tgcttattct tcgggaagct
    35341 atgacacaaa tccatatatt ttattgaatt ggcatgatac gctggatcag ctatttacgc
    35401 ttgtccacga aatgggacat agtgttcata gttatttcac tcgttcgaac cagccttatg
    35461 tgtacggcga ctactccatc tttttagcag aaattgcttc gacaacgaat gaaaatatcc
    35521 taacggagta tttattggaa acagaaaaag accctcgtgt acgggcttat gtactcaacc
    35581 attacttgga tgggtttaaa ggaacagttt tccgccagac acaattcgct gaattcgagc
    35641 atttcatgca tacggaagat gaaaaaggtg tgccattgac tagtgaatac ctaagtgata
    35701 gttatggtaa attgaatgca aaatattatg gtccagcagt cgaagaagac ccagaaatta
    35761 aattcgaatg gtcacggata ccgcattttt attataatta ctatgttttc caatactcga
    35821 ctggcttttc tgctgcttca gcactggcga agaaaatact aaaccaggaa ccagaggcac
    35881 tagagaacta cttggcttac ctaaaagcag gtaacagcga ttatcctgtg gaagtaatga
    35941 aaaaagcagg agttgatatg acacaggctg catatattga agatgcaatg tcgatgtttg
    36001 aacaacgtct aaatgaatta gaagaattga tcgatcgttt ataaggcttt ctatttttga
    36061 ttttctcgaa tagaaacgaa aaaaatacgt cggctctctt gtaagggagt cgacgtattt
    36121 ttt
//
LOCUS       DACDLI010000020        35295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDLI010000020 DACDLI010000000
VERSION     DACDLI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.020"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..965
                     /locus_tag="IV353_001058"
     CDS             15..965
                     /locus_tag="IV353_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9009325.1"
                     /translation="MKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQNKQHS
                     KKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVHITNKG
                     FVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQEMTFE
                     QKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRRIQEQK
                     QFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAHLSLKR
                     QLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1129..1359)
                     /locus_tag="IV353_001059"
     CDS             complement(1129..1359)
                     /locus_tag="IV353_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9009326.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1445..2332)
                     /locus_tag="IV353_001060"
     CDS             complement(1445..2332)
                     /locus_tag="IV353_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9009327.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2421..3644)
                     /locus_tag="IV353_001061"
     CDS             complement(2421..3644)
                     /locus_tag="IV353_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9009328.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3661..4740)
                     /locus_tag="IV353_001062"
     CDS             complement(3661..4740)
                     /locus_tag="IV353_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9009329.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(4871..6598)
                     /gene="ptsP"
                     /locus_tag="IV353_001063"
     CDS             complement(4871..6598)
                     /gene="ptsP"
                     /locus_tag="IV353_001063"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9009330.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6598..6864)
                     /locus_tag="IV353_001064"
     CDS             complement(6598..6864)
                     /locus_tag="IV353_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9009331.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7343..9577
                     /locus_tag="IV353_001065"
     CDS             7343..9577
                     /locus_tag="IV353_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9009332.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9725..10045
                     /locus_tag="IV353_001066"
     CDS             9725..10045
                     /locus_tag="IV353_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9009333.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10250..10474)
                     /locus_tag="IV353_001067"
     CDS             complement(10250..10474)
                     /locus_tag="IV353_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP9009334.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10672..12252)
                     /locus_tag="IV353_001068"
     CDS             complement(10672..12252)
                     /locus_tag="IV353_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9009335.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12653..14029)
                     /locus_tag="IV353_001069"
     CDS             complement(12653..14029)
                     /locus_tag="IV353_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9009336.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14157..15275)
                     /locus_tag="IV353_001070"
     CDS             complement(14157..15275)
                     /locus_tag="IV353_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9009337.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15417..15851
                     /locus_tag="IV353_001071"
     CDS             15417..15851
                     /locus_tag="IV353_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9009338.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15929..16462)
                     /locus_tag="IV353_001072"
     CDS             complement(15929..16462)
                     /locus_tag="IV353_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9009339.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16556..17734)
                     /gene="mutY"
                     /locus_tag="IV353_001073"
     CDS             complement(16556..17734)
                     /gene="mutY"
                     /locus_tag="IV353_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9009340.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17727..18524)
                     /gene="recX"
                     /locus_tag="IV353_001074"
     CDS             complement(17727..18524)
                     /gene="recX"
                     /locus_tag="IV353_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9009341.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18631..20013
                     /gene="rlmD"
                     /locus_tag="IV353_001075"
     CDS             18631..20013
                     /gene="rlmD"
                     /locus_tag="IV353_001075"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9009342.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20187..20765)
                     /locus_tag="IV353_001076"
     CDS             complement(20187..20765)
                     /locus_tag="IV353_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9009343.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21087..21557
                     /locus_tag="IV353_001077"
     CDS             21087..21557
                     /locus_tag="IV353_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9009344.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21665..22891)
                     /locus_tag="IV353_001078"
     CDS             complement(21665..22891)
                     /locus_tag="IV353_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9009345.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23645..23845)
                     /locus_tag="IV353_001079"
     CDS             complement(23645..23845)
                     /locus_tag="IV353_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9009346.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23979..24587)
                     /locus_tag="IV353_001080"
     CDS             complement(23979..24587)
                     /locus_tag="IV353_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9009347.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24672..25142)
                     /locus_tag="IV353_001081"
     CDS             complement(24672..25142)
                     /locus_tag="IV353_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9009348.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25190..26104)
                     /locus_tag="IV353_001082"
     CDS             complement(25190..26104)
                     /locus_tag="IV353_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9009349.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26250..27053
                     /locus_tag="IV353_001083"
     CDS             26250..27053
                     /locus_tag="IV353_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9009350.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27231..28178
                     /locus_tag="IV353_001084"
     CDS             27231..28178
                     /locus_tag="IV353_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9009351.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28246..28539)
                     /locus_tag="IV353_001085"
     CDS             complement(28246..28539)
                     /locus_tag="IV353_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9009352.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(28567..29232)
                     /locus_tag="IV353_001086"
     CDS             complement(28567..29232)
                     /locus_tag="IV353_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9009353.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29372..30004)
                     /locus_tag="IV353_001087"
     CDS             complement(29372..30004)
                     /locus_tag="IV353_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9009354.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(30011..31078)
                     /locus_tag="IV353_001088"
     CDS             complement(30011..31078)
                     /locus_tag="IV353_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9009355.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31075..31806)
                     /locus_tag="IV353_001089"
     CDS             complement(31075..31806)
                     /locus_tag="IV353_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9009356.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(31975..32454)
                     /gene="greA"
                     /locus_tag="IV353_001090"
     CDS             complement(31975..32454)
                     /gene="greA"
                     /locus_tag="IV353_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9009357.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32590..33765)
                     /gene="mltG"
                     /locus_tag="IV353_001091"
     CDS             complement(32590..33765)
                     /gene="mltG"
                     /locus_tag="IV353_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9009358.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            33969..35138
                     /locus_tag="IV353_001092"
     CDS             33969..35138
                     /locus_tag="IV353_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9009359.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT        10623 a         7129 c         5853 g        11690 t
ORIGIN      
        1 tgttaaaaga aaaaatgaaa actctcccca tgctgccggg tgtctatctt atgaaagacc
       61 gagatggtaa ggtcatctat gttggtaaag caaaacggct caaaaataga gtaagcagct
      121 attttcatca gaataaacag cattcaaaaa aagtattacg tatgattcat catataacag
      181 attttgattt cgtcgtagta gacacggaat tagatgcttt attgctcgag tgtcaattga
      241 tccaacatta ccgtcctttt tataaccgac aaatgaatta tttcagtaat tataattatg
      301 tacatattac caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc
      361 cttttcgtct ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa
      421 tgccttggtt gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg
      481 aaatgacttt tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa
      541 agctactgac ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa
      601 aagccggtat gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg
      661 aacaaaagca gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg
      721 agagtcagaa aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct
      781 ctggtgacgt ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac
      841 gtcaattgtc aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat
      901 tagaaaaaga acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa
      961 attagggctc tttgtcaata aggactgatg ggttgtacaa aatcaaatct gagccagaat
     1021 gtggttcatt ctgactcaga tttgattttg cacaacccat cagtccttat tgacaaagag
     1081 ccgcttcttt taggcatctt caacttcaaa tgtcaattat cctaaaattt attggattaa
     1141 gtcatagatt tccatcgcta cgatatcgat attatcaaat tcgtattgct gatgagtatc
     1201 tgtttcagtt aattcaaatg tatcggtcgc tttatcataa gttactgtac acttctctac
     1261 gccttctttt tcaaaacggc gaacttgcag ttcattatct gctgactcga ccatagcctc
     1321 cagtcgtttt atgattgcga ctaattgtga atgtgtcatc ggaggtcctc cttttcttat
     1381 ttctgttata ttgtatcaaa agcatagcct acttgcattg attttctcta aaaaaactcg
     1441 ttttttatga ttttttttct ggtatttttc cttcaggcca ccaaagtttg atcaaagctt
     1501 gggttccgtc attctcaaaa cctttatctg ccaactcttc atatagtttc aatgcttggg
     1561 tagttgctgg caatacaaga tccatttttt ctgcttcttc taatgcaatc tttaaatctt
     1621 tgataaaatg tttgacaaaa aaaccaggtg tgtaatcttc ttttaaaata cgaggagaat
     1681 aattgcttaa agaccagtta gctgcacttc cacctgaaag cgtctcaatc actttcttta
     1741 aatcaagtcc gtttttttgt gcatagacaa gcatttccgt cattcctgtc atcgtacctg
     1801 caatcatgat ctgattagcc atttttgtat gctgaccttt cccagcagaa ccatgcaatg
     1861 taaatgtcgt accaaattct ttgaataaag gcagtacttg atcataaacg gcttcttctc
     1921 caccgaccat gatcgtcagt gtaccattct ttgctcctaa gtcaccaccg gataccggtg
     1981 catctaacgc atctgcgcct ttttcttttg ctgtttttgc aattttttct gccaggcttg
     2041 gtgtacttgt agtcaaatca actaaaatat gtcctgagat atctgcagaa aaaatccccg
     2101 actgtccata gtagatttct tctacatctt tggggtaacc taccattgta aagagaatat
     2161 tactttgttc tgctaccgct tttggtgtat ctgcccagat agctccttct gttactaaag
     2221 gatcagcttt tgcttttgtc cgattataga cagctacttc gtgtcctgct tctagtagat
     2281 ggcgtgcgac agcacttccc atgacgcctg ttccgataaa accaagtttc attttttcga
     2341 cctcctaaaa atcaaaaatc cttgcttcta tgctagtata cgctagaaac aaggattttt
     2401 tcataaaaaa tgtttgaatt ttatctttta aatgaattta attttatttt gattcgttca
     2461 atcgagtctt cttcttcttt tcttcgcatc tcttcttcat gataaacaac catgtcttga
     2521 taaaattcga tcatagcttc gccgaaatgt gtagatgaaa tatcataaag tttagcttct
     2581 aaaacttttg gatctttagg aacttcactg gaaatataat caataagtgc tggtgcaaag
     2641 tcttcgtcgg tttcaaaagt tttccctaat gaaggatgat cgaacaatcg gttcaaataa
     2701 gcatttcctt ccacgacaca aggtgttccg gctgccatcg cttctgtata agtaagccct
     2761 tgtgtttctg aagtagaagc actgacaaaa taatctgccg ctttataata aagtgctaca
     2821 tcattgttgt ccacttctcc tgtgaactgc acaaattctt ctaactgcag ttctgagatc
     2881 aattcctcta actcttctac ataaggtcct tttcctacga taactaatct ggcgttagga
     2941 taagcggaga tgacctgagg aaacccatta atcaaggctt ggatattttt ctcataagaa
     3001 atcctgctta aagaaagaat catcaaatgg ttttcctgaa gacctagtga ctcccttaat
     3061 ttgctgatat cctgctgagt gatatcagaa cgtttgaatt tttcaatgtc gataccagta
     3121 ggaatcacgc gaagcggtgc tattacacca tagctgcgta atgtatcaat aacacgttca
     3181 cttggacaaa ctacacctgt cgtatgattt gcaaataatc tagacaagta ttttacatgg
     3241 gattgcctga tcacttttcc tttaccgata taatgaaggt agtcttcata catcgtatgg
     3301 taagtatgga tcagaggtat tttcaacttc ttagctacca tcttcccaag tatccctgct
     3361 ccgaattctg tatgggtatg aataagatct agttctagtt ctttggcaac caaataagca
     3421 taccacatgc ctctaacaac aactcttcga tccttgaaag aaataaatgg tacacttggc
     3481 attcgaatca catctttttc caacttatcc gcattaggat cggtcgttgt aaaaatatag
     3541 acttcatgcc catgtctttc taattcttgt tttaatgttt tgatcgatgt agcaactccg
     3601 ctgacttgcg gaaagtaggt atcggtaaaa aagccgattt tcatgttttt cgcctccaaa
     3661 ctagtccttg acctgattta ctttttttaa tttgctagag actttttcat tcagtacact
     3721 ttcgtataca tttttaagtt cttcaccgat tttattgata cttcgctctt ttgctgtttg
     3781 ataaccggct tgtgtcgtac tcggtagttc ttgattggta atctgttgga tcagtcggat
     3841 aaattcttcg tttgtcttcc ccatatagca gttcttgcta tcttctaacc agccattata
     3901 cactggaata tctcgaacaa gtacgttttg tttgcttgct aaagcttcta aaacaacgat
     3961 cccttctgtc tcttcataag aagggaaaaa gaatagatct gcacctgaat acgcaccttc
     4021 tatgatctct ccacgaatgt aaccaggaaa caggacattt tcaggatgat cttcttttac
     4081 aattttccgg atgttccgag gaatcgagta catgggaaca tgaccaaacc aaataaatgt
     4141 gtattcgggc atcttcttag caatttctac gaaatcaatg attcctttac gttcgaaaaa
     4201 caaaccgaca caaatgatta ctttttgatc cggagacagt ttgaaatatt ctctgaactt
     4261 ttgttctttc attggatcag ggtgatattt ctctaagtcg atcccattgg aaatagcttg
     4321 aatcggcaca cggataccat agctttccaa caactgtttt gaataaggtg ttggcgtgat
     4381 cagatagtcc gctttttggt acaatcctac cagatatttt tttacgatag gagaaagctg
     4441 gttcgatccg ataaaggaat ttcggaaatc ttcctctgta gaatgcgcat gatagacaac
     4501 tttttttcct tctcttctgg ctttattgac catattatga ctattgattc cgtatgtatt
     4561 gatgtgcagg atgtcataat cttctttttc atctagtgta taagagatac cgacttccgt
     4621 cagagcacgt ttttgatgat caagcgcacg cccgattcca gattttgcaa gcatcttttc
     4681 actttcaaaa tataataata ccttcacctt acgtccccct tcacttatat agtataacat
     4741 agcctacaaa ctgtcactca aacttaagcc ctatttttgt ctattcttac tgacaaaaac
     4801 gttttcttaa aaaagaacaa aaaaactggc gtagctcttt tacaagccac accagctttt
     4861 gatacgttgt ttattttaca tattcatgaa caagttcaac gacttcttcc atcatatcgc
     4921 attctttcaa tgcacgatca gcaagttcag ccattttcgc tgtgtccaag cgtttcatta
     4981 aactacgcgt tttaagaata gatgtcgcac tcattgagaa ctcatctaga cccataccta
     5041 ctagcaatgg aacagctgtt tgatctccag ccatttctcc acacatacct gcccattttc
     5101 cttcagcgtg tgctgcatca attacatttt tgatcaaacg taaaattgat gggttatatg
     5161 gttggtacaa gtaagaaacg cgttcgttca tacggtctgc tgccattgtg tattgaatca
     5221 aatcgttcgt acctacagag aagaaatcta cttcttttgc aaacttgtct gctaagactg
     5281 ctgctgctgg aatctcgatc atgatgccga cttggatcgt atcagatact tccttgcctt
     5341 cacttatcaa tttttgtttt tcttcttcaa agatcgcttt tgctgcgcgg aattctttga
     5401 gtgtagctac catagggaac atgatacgta aattaccatg aacagatgca cgaagcaatg
     5461 cacgcatttg tgtacggaac atttcgtcgc cttgttcaga taaactgata cgcaatgcac
     5521 gatatcctaa gaatggattc atttcatgag gtaattgcag atatggtagt tctttatctc
     5581 caccgatgtc cattgtacga acaactacag gttttccttc cataccttct aatacagctt
     5641 tgtaagcttc atattggtca tcttctgttg ggaagtctgg agaatccata tacaggaatt
     5701 cagtacggta cagaccaact gcttctccac cattattatg gacaccaact agatcttttg
     5761 gtgttccgat gtttgcagct aattcaaaat gcttgccgtc agctgtgaca gtttctgcat
     5821 tttttaattt ttcccattct gctttttgtg ctgcgtaatc tgcacctgct ttttcaaatt
     5881 cagctttttg ttcatctgta ggatcgatga ttacatcgcc ttcgatcccg ttgacagcca
     5941 aaatatcgcc ttctttgact ttagcagtaa tttcttttgt cccaacgatt gctggaattt
     6001 ctaaagaacg tgccatgata gcagaatgtg aagtacgtcc accaatgtct gttacaaacg
     6061 cttttacaaa attgcgatct aattgtgctg tatcacttgg agtcaagtca tgtgcaacaa
     6121 cgaccacttc ttcgttgatc attgatggat ttggtaaagt aacgcctaat aagtgcgcta
     6181 aaatacgttt agcaacgtca cgaatatctg cagcacgctc ttgcatataa gcattatctt
     6241 ccattgcttc gaacataccg atatacatat ccgttacttc ttttagcgct gattcagcat
     6301 ttacgctgtt gtctttaata ttttgcttga tttgacctac catttctgga tctgaaagaa
     6361 ccatcaaatg ggcatcaaat acttgcgctt ctgcttcacc taagctttgc gctgcttttt
     6421 cacgaatttg ctgcaattct tcagtagatt tagctaatgc agcatccaaa cgagcttctt
     6481 cagccgcagt atcttctact gttgccttac tgaatgttaa atccggttga actagcaggt
     6541 aagcttttgc aacggctaca ccgtcactag cggcaatccc ttttagcatt tcaaccatta
     6601 ttcagataag ccttctttct tcattgtttc aacgattgct gccattgcat cagcttcgtc
     6661 agcaccttct gcagtaatag taacatcaga accttgacct acacctaaag acataacgcc
     6721 catgattgat ttcaagttta ctgatttacc tttatactct aagtttacat cagagttaaa
     6781 tttgcttgct gtttgtacta atagtgtagc tggacgagcg tggatcccag tttctgctac
     6841 tacgtgaaat tctttctttt ccatattaat cggtctcctt taagtgagtg ttgtttttat
     6901 gtgagggttt tcttttttag taattcagag aatcctaaca aatgccaccc tttcatctgt
     6961 aaagattaac attttttttc gttagataca acagttttca ttcaatttca gataaaagta
     7021 ttcaaattat tccggattct ttttcatcct tttcttcaca ttttataaca aacgtgttta
     7081 tttccttttt cttgtcgctt tctcttctcc tgacgtttat cattctttcc tttgaccctc
     7141 tttgttttca tatgaaacga attacttgct tcccttttta tttttagtat aataagagtc
     7201 aaaggtcagg aatggtcaat agagatttca ccttttctac ctcataaatc tcttaaaaaa
     7261 ctaggggtta ttcttttacc tcatgaaaaa cgagatgtaa aatatggatg tacccatttt
     7321 tgtgaaagga cgtgaatgat tcatgctttg tcaaaactgc ggcaagaatg aggcaacgat
     7381 tcacttgtat gccactgtca atggtcagcg gacacaactc gattactgtc agagctgtta
     7441 tcaaaaaatc aaaaatcaac aaaatggagg tctgactaat atggcccaaa atgatccatt
     7501 tggatttggc agtttggacg atcttttccg ttccatgtct cgccaaatgc aacagcaagg
     7561 aaattatgag caaacaccac caacacaatt tggcggaaac aacaacttca atggtggtca
     7621 gccacctcaa ggcaatacag aaggtttatt gggagaatac ggaatcaata tcacagaaca
     7681 agcacgacaa ggtgatatcg atcctgtgat cggacgcgat gacgaaatca aacgagtcat
     7741 tgaaatttta aatcgccgaa ccaagaacaa tcctgtcttg attggagaac caggtgttgg
     7801 taaaacagca gttgttgaag gtttagcaca aaaaatcgta gatggtgatg taccgcaaaa
     7861 attaatggac aaagaagtca ttcgtttaga tgtcgtatct ttagtccaag gtactggtat
     7921 acgaggacaa tttgaagaac gtatgcaaaa attgattgaa gagatccgtc aagcagaaaa
     7981 tgtgatccta ttcatagatg aagttcacga aatcgtcggt gctggttctg ctggtgatgg
     8041 aaatatggat gctggtaata ttttaaaacc agctttagct cgaggagaat tgcaaatggt
     8101 tggtgctact accttaaatg agtaccgcat catcgaaaaa gacgctgctt tagaacgtcg
     8161 tatgcaacct gtacgggtag atgaaccaac tgtcgaagaa acgatcagta ttttgaaagg
     8221 tttgcaaaaa cgttatgaag actatcatca tgtaaaatat actgatgagg ccatcgaagc
     8281 agccgcaact ttatccaatc gttacgtcca agatcgcttc ttgccggata aagccatcga
     8341 tttgctagat gaaacaggtt ctaagaaaaa cttgacgatc caaatcgttg atcctaaaac
     8401 aatcgagaaa aaactgcaag aagcagaaga acaaaaagtt ttagcttctc gagaagaaga
     8461 tttcgaaaaa gcagcttatt atcgcgatca gatcaataaa cttcaaaaaa tgaaagaacg
     8521 tcagctcact gaagaggaaa cacctgtgat taccgaaaaa gatatggaaa aaatcgttga
     8581 acaacggaca ggtattccag tgggtgaatt aaaagaaaaa gaacagacac aactgaaaaa
     8641 cttagcagat gacttgaaag ctcatgtcat cggacaagat aacgctgtag atcgagtagc
     8701 gaaagcgatt cgccgaaacc gtgttggatt aaacaaacag aatcgtccaa tcggctcctt
     8761 cttatttgtt ggaccaactg gtgtaggtaa aacagagctt gcgaaacaac ttgcctacga
     8821 actattcggt tcacaagatt caatgattcg tttcgatatg tctgaatata tggaaaaaca
     8881 tagtgtctct aaattgattg gttctcctcc aggttatgtc ggatacgaag aagctggtca
     8941 attaacagaa aaagtacgga gaaatcctta cagtcttgtc ttgttagatg aagtagaaaa
     9001 agctcatcca gatgttttgc atatgttctt gcaaatctta gatgatggtc gattgacaga
     9061 tgcacaagga agaacggtca gctttaaaga tacgatcatc attatgacaa gtaatgccgg
     9121 aactggtaaa gtagaagcaa acgttggttt tggtgcggca cgcgaaggtg ttactcgttc
     9181 tgtattgaac caattaaaca actactttac accagaattt ctgaaccgtt ttgacgggat
     9241 catcgaattc agtgcattat caaaagaaaa cttgatgaca atcgtcacac taatgctaga
     9301 cgatgtcaac caaatgctgg cagcacagca acttcacatc gaagttccta cgaatgtcaa
     9361 agaaaaatta gttgacttag gctatgatcc atctatgggt gctcgtcctc ttcgacgcac
     9421 gatccaagaa caaatcgaag atggtatcgc tgaattttat cttgatcacc cttctatcca
     9481 tgagttgaaa gcaaaactgg ataaagatgg aaaaatcatc gttacttcta aaccagaacg
     9541 tttggctaaa gaatcagccg aagaaacggc tgaataaatc cagttttata gcgtgagacg
     9601 gtatttcttt gagaaatgcc gtctcattct tttttcagaa aagtcaaaat actgttcatt
     9661 ttataaaaaa gaaattataa taagagaaac aagcaattaa aaatacataa aagaggtgag
     9721 cgccatgaaa ttggtaaatg taacaaacag ccattcccgt cttgtattaa accagcttga
     9781 aaacactgat gcccatctag tcaaggtcta cacagccgga aataccacta ttgtctacac
     9841 tgaagcccct gaacataatg aaattctgct gatcaatgac aaacgtaaaa tccagccgaa
     9901 agaaatcgaa gatgctaaaa attatttctt agataaaatc aaagacgccg tttaccacga
     9961 ggatgaaatc aaagtaatcg aattggcagg ccttgttgaa atctcgattc caaaacatga
    10021 attaggcgct acattagccg tgtaaacaaa ttttgaacat aataattaaa gagcgtggga
    10081 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg aaagcagaag
    10141 caactccttc ggaaataagc tgaaattcac gaaaattgct tttcaaattt ttgtgaattc
    10201 cagcttattt cttggagtta aacgcttctg tctcagcctc tttttctttc tagctttttg
    10261 atttttctct tttcttttgt acttttctct tttcattttc agttctacgc gtgtctttat
    10321 ttttaacttt ttcctcttta tggatgattg atcgtacttt ttgtgcaatt tgctcgtttt
    10381 tgtcccagtc ttttacatct aataatcgac ataatcgtaa catctcttct tttttctgtt
    10441 tttttctttc acgtctttgc cattctgttt ccatgtgtca cactctcctt tctttaatca
    10501 tatgtacgca aaataatttt tatatttttt tgattagaca tttctatgat tttaatatag
    10561 taaaaagagc cgtcacggaa aatccgtcac ggctctaaac aaaatatgag aggctgtttt
    10621 tgtttggcta ttcttattac ttgatgcagg gcagcttgtg tcaagacttc tttacatcaa
    10681 actttttaat tttacattgg gatatttatc cgcaaaccag cgttctgcaa attggttctc
    10741 gaataagaac aacggttggt cgaaacggtc acgagccaag atattgcgac ttgaactcat
    10801 cttctcatcc aaatcttctg gatcgatcca gcgagcgatt ttgtgtccca ttggagacat
    10861 gattacttcg gcgttatact catttaacat acgatattgg aatacttcga attgtagctg
    10921 accaacagct ccaatgatat attcttccgt tagatatgtt ttataaagct gaatcgctcc
    10981 ttcttgaact aattgataga ttcctttgtg gaaggatttt tgtttcatga cattttttgc
    11041 agtcactttc ataaacagct ctggagtaaa tgaagggagt tcttcataag caacttttag
    11101 ttttccttcg aacaatgtat ccccaatctg ataattgcct gtatcatata caccgatgat
    11161 atcgcctgct actgcttttt cgacgttttc tctcgaatct gccataaatt gtgtgacatt
    11221 gcttaatttc agttttttat ttgtacgttc caaccagaca tccattcctc gttcgaatgt
    11281 tccagaacag attcggacaa aagcgattcg gtcacgatgg gctggattca tatttgcttg
    11341 gatcttgaat acgaaacctg aaaattcttc ctcataagga cttaccatct gatcatcttg
    11401 tgttttgtgt ccataaggag ctggtgccaa ttcaacgaaa gtctctaaaa aagtctgtac
    11461 accgaagtta gtcaatgcag aaccaaaaaa tactggtgtc tgatcgccac gagcaatttt
    11521 ttcttgatta aatgcatctc cagcctcttt gattagttct acttcttcta agacttgttc
    11581 atataaggaa ttttcagtta gaggatgatt ttcgggaagc agtccatctt catccaattc
    11641 tactaatcgt tcatcctgat aattttccgg acgataaaac tctacacgat tatggtagat
    11701 atcataaaga ccttctagcc ctttacccat accgattggc caattcatcg gataagattc
    11761 gatatctaat agctcttcta attcttctaa caattccaat ggttcacgac catcacgatc
    11821 tagtttgttg ataaaagtaa atatcgggat gccacgtttt tttacaactt ggaataattt
    11881 ctttgtctgt gcttcgatcc cttttgcact atcgattacc atgaccgcac tatccacggc
    11941 catcaaagta cgataagtat cttcagaaaa atcttcatgt cctggtgtat ccaagatatt
    12001 tactcgtttc ccttgataat caaactgcat aacagaacta gtaacagaaa tcccacgttg
    12061 tttctcgatt tccatccagt ctgatttggc aaaattacct gtttttttgc cttttacggt
    12121 cccggcttga cggatagccc caccaaacaa tagtaattgt tctgtaatcg ttgttttccc
    12181 agcatccgga tgggaaatga tcgcaaaagt tctgcgattg tccacttgtt gctttaattt
    12241 tggattattc ataacctaac tccatttctt ccattcaatt atttatattt tctctaggca
    12301 cagattgtcc gatctcttaa agaaagacaa tttcatatag ggctgtgaca aaagccgtgt
    12361 agcagtctct ttttccgagt aaacggcgtt ctagagacag cctatcagca ataagcccac
    12421 ccaagccaaa aatacgaaaa gctgtttttg cttggctgct cttattactt aatgcaggat
    12481 agctttttca caacctttgt caactactac aactgaatca gtatagcatg gattcagcga
    12541 atgaaaaagc caccaaacca taagagtcaa atattttttt gtcagctgac taaaagaaaa
    12601 gacgtgttgc ttgtttattc aagcaacacg tctttcataa ccaagtttcc aattatcttc
    12661 ttggctcgtc atcttcgaat tcttttctga agtatcttcg ttcttcttca ggttccgctt
    12721 ctgtttcttc cggatcatga aaaatgacac ggattttaag gattcgagaa ccttccattt
    12781 cttcagaaac cagcgtaata ttatcgacat caaacgataa cttttcgcct tcatccggaa
    12841 tcatgcctaa tgctgtaatc aaataaccag ccatcgtatc tacatcactc atatgcagat
    12901 cactgtcaaa agcttcattg aactcatcaa tcagcatacg tccttggatg atatattcgt
    12961 gttcatcgat tttttcataa agattctcaa cttcatctga ttcatcatcg atttctccga
    13021 cgatttcttc taaaaggtct tctagtgttg ctaacccaac gacgccgcca tattcatcta
    13081 ataaaatagc catctgattc tgcgtctttt tcatttcata taacagatca tcaataaaga
    13141 ccgtttcagg aacgaataaa ggctcctgca tgattttctt gatatctaaa ttatcaaagc
    13201 cgaatttatg ggcagctttt agtaaattct ttgtatgaag gattccgact actttatctt
    13261 tgtcttcgtt ataaacaggg attcttgaat agttttcgga taaaacttca ttcacatttt
    13321 cttccacagt gtcgttgata tcaatcataa atgcatccgt tcgtgggacc atgacttctc
    13381 gggcaacttt cgtatcaaga gaaaaaaccc cttggagcat ttctaattct tcattattca
    13441 atacgccttc gttttcaagc atataacgca tttcatctct ggtcattttt gaatcttcat
    13501 catcaaaagt catcggcgtg atacgcgcca ataaacttgt ggaagcagaa agcagccaga
    13561 cgaagggctt agctactaca ccaatcaagc gtatcagccc tgaagtgaac tttgcgactt
    13621 cttctgattt atttaaagca atacgtttag ggtacaattc tccaaaaaca atcgatacat
    13681 aggtcaaaat agccagcaca atgatatttg caatattttt tgccgctgtg ccgccaccta
    13741 ataccggagc taaccgttcc gataaagtat tagctagtga agcaccagac aaaatgttga
    13801 ccaaagtgat tcctacttgg attgtcgata ggaaattatt tggattctgc aatattttta
    13861 aaagcttttg agatttcaca tctccttcat cagctttttg ctcgattcga ttcttattga
    13921 tcgaaacaac agcaatttcg gaagctgcta aaaaagcatt gatcaatgtc aaaacgatca
    13981 ataataaaat ctgcgctaac agcgactgac tctcagggtc agcattcata atcgcattct
    14041 ctccttataa aattaatttt tagtttacga tagaaaaaaa cgtggcacta tagccacgtt
    14101 aaaatggtat cacaaagcaa ttttctttgc aataaaaaca agtgtactaa aactacctac
    14161 gaggtcgtag caattttctt cttgttttca ttttgcttgt cttctttgac gattttgatt
    14221 atataggaaa tgatcgtctt gcagattgcg taaaaaggaa cacctaagaa gataccaagc
    14281 aatccggcaa tatttccagc tactagaaga acgataatga tcgttaatgg atggattttc
    14341 agtgatttcc caattacatt aggataaatg atattcccat ccaactgttg gacgattagc
    14401 acaaccacac aacaaagtaa tgcttttacc ggatcattga agactgttac tagaaatgca
    14461 ggtgctagtc ctagataagg tcctaaatat ggaattaggt ttgtcaatcc cgcaatgacc
    14521 ccaaataaga aagcataatc cacaccaatt accatatatc ctaaaaatgt gaaggtacca
    14581 acgaataaac attcgattgc ttgtccgcta atgtaattag ccaatgtttt attcagttgc
    14641 cccaataact ccacaatttg gtttcttctt ttttctggga aaaactgttg gatgtttggc
    14701 actagttttt ctccgtcttt caacatatag aacaagatga atggtgctgt aatgattact
    14761 acggttgccg aagcgatcgt tccaacgatc gaacctaaac tattggatac tccactaagg
    14821 aattgctgaa tgattgtccc gtaagaaatg tctaattgct caaaatattt attgatatct
    14881 acttccttga acaaaggcaa ttcggctacc tcttttaacc agctttctac ctgtttgatg
    14941 aaatcaggca tattagatgc caatgaggaa atttgctgta ctagacttgg gatgacacta
    15001 agtaaaatcc aaataattgc tgcaactaaa aggagcagca caattgcgac agcccatatt
    15061 cttttgattt ttgctttcat caacagcttg acaataggat tcaataaata gtaaagaaag
    15121 cctgcaatca gtacaggtgc aaataacgtg gaaaaaaatg tcccgatcgg ttcgaaaata
    15181 aagtttattt ctgatgaaat catgatcaat gcagctatga tcaataattc aagcgaccaa
    15241 aacattagtt tagactgtct aagtttttca aacaatagat ttcctccttt ctttcttact
    15301 agtatatatt atattcgtta aaaattcttt tattcttttt ttatttttga aaacttgcca
    15361 tactttttat ttgttttatt ttccagtata ctatacgaaa ggaaggtggc tagaccatga
    15421 aagttgtaca aacaaaagat acaatgagca atatttactt agatgctgta cggatcaggc
    15481 atcaagtttt tgtagtcgaa cagggtgtgc ctttgtctcg agaaatagac aaagatgaag
    15541 cccattgtat ccacttcgtc ctgtactcag ataaaaaaga accgcaagga actgtacgtc
    15601 ttttgccttt agaaaacgga aaaatgaaat tacagcggat ggcgattctt tctgaataca
    15661 gacaccaagg gttaggaaaa atattgattg aagaagcaga aaactttgct aagaatcaag
    15721 gctataatac gattttatta ggggcacagt ccacagctga aactttttac gaaaaactag
    15781 gatatacagc ttacggcgat ccttttgaag atgctggcat gcctcatatt tatatgaaaa
    15841 agactttata aaaatttatt tcgaaaaaag caaaaaacag caagcttttg agaaaaatct
    15901 ctcactggct tactgttctt ttttgtgttt acttacgcga caattgcttg taacggttat
    15961 accaaatatc aatatagcct tccgagaacg ggccatctcc attattgatc cagtcaacca
    16021 gaatcttcac attttctttt aggatgaagt caatatcgtt cgggtaattc atcattttgc
    16081 tgtgcatctc atattcatcg acatccaata gtcgtttctc gccgtcagga aataccttga
    16141 tatccaagtc atagtcgata tatttcaagg cctcatcgtc cagcaaaaaa ggtgaagcta
    16201 gattacaata ataggaaacc cccttttctc tgatcattgc tattacattg aaccagtatt
    16261 ttttatgaaa ataaacaatc gctggttcgc gcgttaccca tcggcgtcca tcagattcag
    16321 tgactaatgt gtgatcattc acgccgatca tagagtgttc gcttgttttt aataccatgg
    16381 tatctcgcca cgttcgatgc aatttgccgt cgtgcttgta actttggatc gtcacaaact
    16441 ctccttcttt tggaactccc atagcaaccc ttccttaagc agcattttgt gctttgtgtt
    16501 tgggtgttca ttacaccgac atcataatta ttatatcaat aaaaatgagc tttgcctaga
    16561 aaggattcct attgtttcga tttttctcta actgatctac cagtttcatt tggaccttag
    16621 gaaaaacgac cgaatcaaat gcatcaggct tcaaccactt cgaagttttg ttctcggtaa
    16681 attcttgttc tgctccctcg gttgcccgtc cgtaaaaaag gagaacatgc catttcagat
    16741 gactaaaaat atgagtaact tctcctaaat gtcgtgtctg ccaaacaaag agttcctgct
    16801 tttcaaatgg gagattttgc tcttcttctg ctactaggtc atcaaacaga tcgatttctg
    16861 gctttgtttc ccattctttt ttcaatcgct catactcctc ttgtgtcact tccatcattg
    16921 gaaatgtcca catattcgcc agcaattttt ggctatctct ttcttcaaaa taatacgctc
    16981 cagaatgatt ttgaagagct gcactgatat aataaacgtc ttttggcttt gctttttttg
    17041 tttttacagg aaaactcgtt tggattcctc gtttgttagc taaacaaaag gcttggatcg
    17101 gacaagtctc acactttgga gaagttggtg tacagatagc tgaacctaga tccatcattg
    17161 cttggttgaa ttcacctgga tgcttttcat caatgatttt ccgcatcgct tcatcaaaga
    17221 ttttcctgct agaagctttt gcgatgtctg cttcaatgca aaacaatctg ctgactactc
    17281 gcatcacgtt gccgtcaact gcaggttctg gaagtccaaa tgcgatactt gcaatcgcgc
    17341 ctgtcgtata tggtcctatt cccttcaatg agctaatttc ttcaggcgtt tgcggcattt
    17401 ccccatcaaa ttctgacatg atctgtttag cagcagcttg gatgttccgg gctcttgagt
    17461 aataaccaag gccctcccat gcttttaaaa gcttttcttc tggagcattt gctagctctt
    17521 caattgtagg aaaccattcc atgaaacgat aaaaataatc gatcactgtg tctactctgg
    17581 tttgctgaag catgatctca gagatccaga tacgataagg atcacgatta tagcgccaag
    17641 gcaggtttct tttttcttgt tcataccatt gaatgaactg atcttgaaat tcttttgttt
    17701 ctttatctgt ccatttttcc cattctttat tcactttcct cttccttttc cgtaatgaat
    17761 cgctggatca tatccaaatc aaatcctttt tgatataagc tttgctttac ttttagattt
    17821 ctttttttag gttcaaacct taaatttctt cgccatagtt tatctccttg tttgactaaa
    17881 taatcatatt ctatttcttg atctgcttct tgcggaagat cagctatcgc ttgttggatc
    17941 acttcttgag aaaagccttt cgtcatcaat gtttgttgga tttttctcaa taattctttt
    18001 tggctttttc cgtgattttt tccaaacgct ttttcggctg tctgatgtgc attttgaact
    18061 tggtcttcaa atgtatattg gctgagcgct tgttcaatga tttcaggttt caaacctttt
    18121 tgctgcatct gctgcgctaa tttctttggt cctttgtcac ttaagcgcat attcgtgcgc
    18181 acataacttt ccccatagac caaatcatcg acaaggtcca attcttttaa gcgggctacg
    18241 attttatttc gatcttcttg aaggatttcc ttttctttta agtatgttcg gatttctttt
    18301 tccgatcgca actgataact taagtaattc attgctaggt gaaaaccgaa atcagctccg
    18361 ctactcttct tgatctcttg gatagtttct tcgtctaatt cccgtccttt taataatcga
    18421 tagcgtacta atacatcttc agatacttgt aaagcttctc cttcagataa atctacgcga
    18481 tagaaagggc ctttgccttt actgattctc gtaacggtta acataaacat cctcctcgaa
    18541 catatattct ctatcatacc acagaaaatc cccgtaatca ggtattatat gttagaataa
    18601 accacagaaa aaagaaaaag ggagatactt atggcagaaa caattgtcaa aactggacaa
    18661 acggttcctt tgaaaatcaa gcgtttaggc ataaatggtg aaggaatcgg ttatttcaaa
    18721 cggttgatca tatttgttcc atatgcacta ccaaaagaag aagtactagt gaaaatcacg
    18781 aaagcaactc ctcgctatgc agaaggagaa ttaataaaag tcaaaaaaac aagcaaagat
    18841 cgcgtagtgg caccatgtcc tgtttactat gaatgcggtg gctgtcaatt acaacactta
    18901 gcttatcaag ctcaattgga ttttaaaaag gatctgcttt tgcaagcatt ggaaaaattc
    18961 aaaccagcag gctttcgatc ttatcaactt ttgccgacga tcgggatgga tgagccttgg
    19021 cattatagaa acaaagcaca atttcagtta cgaaaaaata agcagactca aaaaattgaa
    19081 gctggtttat acgaagcaaa ctctcatcag ttagtcccta taacagactg cttggttcaa
    19141 gaacctcgaa cacaaaaagt aatgaatact gttgttcaac tattgaataa atttgatgca
    19201 cccatctatg atgaacggac aaacagtgga atcttccgca caatcatggt tagaatcggt
    19261 gttaaaactg gggagttaca agttgttttt attactcgca gtaaaaaatt cccgcaaaaa
    19321 aacgcaatga tccgtgagat caatgaacga ctgcccgaag ttgtttcaat catgcaaaat
    19381 gtccaggcac aaaaaacgtc tctcgtgatg ggggatgaaa cgatccactt atggggaaaa
    19441 gaagccattg aagaacaaat caatgaagtg actttcgatc tttctcctcg tgccttcttt
    19501 caactcaatc ctcagcagac cgaattgctt tatggcgaag gaatcaaagc attagatgtc
    19561 cagcctaatg aaacgatcgt ggatgcttat tgcggagttg ggacgatcgg cttaagtgta
    19621 gcaaaacaag ccaaagaagt acggggaatg gataccgttc ctcaagcaat cgctgatgca
    19681 aaaaaaaatg cggaaagatt aggatatcac aacactcgat atgaagtcgg gacagcagag
    19741 caagttctgc ctaaatggtt gaatgaaggt tttcagcctg atggtatcat cgtagatcca
    19801 ccacgtacgg gtttggataa cgcattgatc caatcattat tgaaataccc tccaaaaaaa
    19861 cttgtttata tctcctgtaa tgtttctaca cttgccagag atctggttaa tttaagtaaa
    19921 aagttcaaga ttgaatattt acagtccgtc gacatgtttc ctcagactgc acgttgtgaa
    19981 gttgtcgtga agatgacgag actcgctgac taaatgaaag aggttgtgaa aaagtctgcc
    20041 ctgtatcaag taataagaac agccaaacaa aaatagctct tcatgtttta tgaatactgt
    20101 acttgtgacc gaagagttaa caggaggaca ctatttattt aaaaaaaggg ttgtgacata
    20161 gtttttgtca cagccctttt tgattattat tggatattca cgtctatcac ttgataaaat
    20221 gcatttgtcg tatcgctgat attccatact gctaaaatca cattatatcc ttttcgatcc
    20281 tgcggaagtg tgatcgtttg gttgactgtt tttggtggaa cagttgcttt gtcgtctatt
    20341 tttgtgatca attcaaaatc actaaatttc aaaggttgat taggattcca agttggtttg
    20401 gtaatgtagt aatcccaagt acttgtccga tgttgtgctg ttaaattcca agtaatattt
    20461 aatgttcctg gattaaccat gtttttatgc catctgttag atgtctgttc atctaaaggc
    20521 tcaaagccgc tgactcctgc actagcaatt tttccatcaa tgaatgtatt tttggtagct
    20581 tcgatacttt gcggttccca ttgtgctctt ccgacatttt gatttaagtt tcctgctgct
    20641 gatgttccta ggtatgcacg gcttcctgga gaagtaatat aaccatgcgc aaaacctgga
    20701 atgctaaaac cgaacaaccc taaactaact aacccaataa acaataacgt actaatcttt
    20761 ttcatgtaga aacctccttc atttgataac cacattgtaa acgctttatt gtattaacac
    20821 aaacattatg cttttataat tttatgttga agtaaaaaag gtgaaaaaag taagatgcag
    20881 attataacag aagtagacga gaaaagtaca aatagaaaac atgagaatca aatcatatga
    20941 tctttttcaa aaacatcatt catataaaca aatgttcaac tttttaataa caacaattat
    21001 caattttttt cttacttcac aaactaaata caggtcttgc tgtgagcctt catggtaaac
    21061 taatattata gaaaggagtt agtaaaatga agatgataaa agatattcgt ttttgggttt
    21121 gtgttatagg aattgtcata ttaggctttt tatcagggtt gcttagcgga aatcccggaa
    21181 aatattacta ttctttgcaa cttccaccat ttgcaccacc tagctggata tttgggccga
    21241 tgtggacctt actgtatata ttgatgggta tttctcttta tttgttactt aatcacaaca
    21301 acaaaaaaca gcgaaacaat cttgtcggtc tttttgtgat tcagttcatt tttaatttta
    21361 tatggtctgc tctatttttt aatttaagaa atattttcat tgcagcaatc gatattacac
    21421 ttttagttat tttcttgtca gtgcttatgt atcagttgtg gctgcatcat cgcctggcaa
    21481 tgtggctcat gattccttat tatttatggg tactattcgc aactctattg aattattcaa
    21541 tttatttttt aaactaagcg ctaaaaagag acgtcctgta atttaacaca tagaaaaagt
    21601 gccacctaag ttagattttt tgtctaattt aggtggcact ctttattaac caaactattt
    21661 ttttctagta cccacttgtt gttccatact cattattctg gtcataacca taaccatttt
    21721 gttcctgtcc agcgtaagga tcataacttt gagtatcttg acttggtgta tagtactgac
    21781 ttgtatcttc tgtttgctga taataggtat tcggatcttg gtactgatca tatgtgtttt
    21841 ctgaatagga agaataatcg gtgtaccctt cactattcga tccatcaccg tttgtcgaat
    21901 cgccatcttc cgaatcatca aagaacatca ttgaagcatt ctcatttttc aaattaggta
    21961 atgtatcgct tgtttttata tttaattgtt tcttcagttc attttggatc gaaagaagat
    22021 cattttttcc taaaatttga tagtagatat cattgatcat ttgtccttcg ccttgcaact
    22081 ggtcttgttt gattgtttca aacgccgggg tgtagttcgt cgctacatca agcatgtcat
    22141 cccaatctaa gtccgtcgtc acattgttct caactgcttt taaaattttt ctataattgc
    22201 ttacgccatc taaacttaaa actttattga cgattttcgt cactacttcc cgctgacgac
    22261 gttgtcgacc gatatcgcct tctggatctt cgtgtcgcat tcgagaatag gctaaacctt
    22321 tttctccatt caatgtttgt tcacctactg gtacatggac accatccaaa gtaaagtcaa
    22381 ttttattatt cactttgatg ccaccgaccg cattgatcaa atcttccatg ccatccaaat
    22441 tgatcgttac ataatgatca ataggaatat cgagcagttg ctcaattgaa tccatggcca
    22501 tttcgacacc accaaatgcg taggcatgat ttaatttatc taccgttcca taacctacga
    22561 tattcgtata aatatctcgg tccaaactga tgatcgttga ttttttttgt ttcggattaa
    22621 ctgttactac catcatcgta tcagaacgtc cttgttccgt tcttcctaat ccaccggtat
    22681 ctaaacctaa tagcaaaacc gaaaaaggtt ctttgtcatc aatgcttact ctctcagaac
    22741 gtttggagga tattcgatta gagtttttag aaattttgtt tactgtttgg ctcgcttctc
    22801 ccataaatct gattccgtat gctgataatc cggcaatcag caaaacgaat acccctaaca
    22861 aggttaatag aactttttgc catcgcttca taatacacct ctgtttcaca ttttctaaaa
    22921 tattgtctca tataactgtt caatcagcaa attcactttt taatcataag aaaactttaa
    22981 aaaacatttt tgtgtaacga ttattatttt ttaaaatttc tcaaaattct taactatcat
    23041 accataccgt gaaaaagatc gaaagaccga atacacgcag ttcgcaatcg ttttatcttt
    23101 ttttatgtat tttttccaga aaaagcgaac aacaaggtat tttccaaata atcgtgcagt
    23161 aaaatgatcg cacatacctt tcccatcttt ttaacagtct ttgcctaccg ttcagattat
    23221 cattcgattt cacgacaatt tcttcctatg actggtcctt ttgttaaacc catcttctat
    23281 tcgttttatt tccagctcac tgcatggctc ttaaaatgac tcgaactagt gattgcatta
    23341 ccatcatctt ttaatggata gatatgctcc tcttcaaagt aaaacgattt tcttttaaag
    23401 taatagaatt gacatggtaa tgagtatgta tactagtggt ttcacaaatg gtcttttatt
    23461 caacatccaa aaactgaaat ttctttaaaa tccattacta acgaagaaaa tgttccttgc
    23521 ttgataatta gttaggacat gtcaagtgaa caaatgcatt tacttagcca aaaaatctaa
    23581 aaacttcata aatagacaaa aagacacgac tctctaagaa gtcgtgtctt tattcaacaa
    23641 attattataa tttagtcact tcagcagctt gaggtccgcg tgcaccttca acaatcgtga
    23701 attctacttc ttgaccttct tctaggctct tgaagccgtc tccttgaatt gctgagaaat
    23761 gaacaaatac gtcatctccg ccgtctactg taataaaccc aaatcctttt tcattgttga
    23821 accatttaac cgttccgtgt tccataaatg tatatcctcc aaaaaagttc ttgcaagtaa
    23881 ccttgcaaga acattatacc caatttagaa aacgctagca agctttcgct cttttatttt
    23941 ccaaattatt tttaagactg atatgacgga ggaaatggtt attttgctgc ttcgaaacgt
    24001 ttgtttactt catcccaatt aataacattc cagaatgctg caatataatc tggtcggaca
    24061 tttttatatt tcaaataata agcatgttcc caaacgtcca aacctagaat tggtgtcttt
    24121 ccttccatca atggagaatc ttgatttgca gtagaggtaa tagctaattt tccattttcc
    24181 attacaagcc aagcccatcc agaaccaaat cgtccagcgg ctgctttttt gaattcttct
    24241 ttaaaagaag aaaaatcacc aaaagcttca ttaatcgctt cttttatttc tcctgtaggt
    24301 tcgccaccag catttggtgc catgatttcc cagaaaaatg aatggttagc atgtccgcca
    24361 ccattgttac gtacagctgt cttgatatct gttggaatag cgtccatatc agataataat
    24421 tcttctattg ttttttcgcc taattctggg tatttctcaa ttgctgaatt taaattcgtc
    24481 acataagtat tgtgatgctt atcatgatgc agatgcatcg tttcttcgtc aatataaggt
    24541 tccaatgcgt catatgcata aggtagatct ggtaatgtgt aagtcataaa aaaattcctc
    24601 ctcaatgttt tcttaataat gtctacatta caaagagtaa caaagagaag gaaattcttc
    24661 aattgttatg cctagtcgtc tttttcactt tgacctactt cttcttcaaa aacctcagtt
    24721 tctttaaaaa taaacaactt gctgaaaata tagttcgcaa ggataacaat aatttgagtg
    24781 atgattttcg ccacatactc attgctgttc ataacattga tcaacaatac cattgccccc
    24841 atgtccaatc cgtaagaaag gatacggtag aagaagaact taccgcattc aataagtgca
    24901 tgtgtaaaat tcggtgattt tgaatggaat acccaaagtt tgttcgttac aaaagcaaac
    24961 aatacagaac aaaaccacga aatagaattg gataaggcca tacttaaatc aaaatattgg
    25021 cgtgcgagag caaaactaac aaaatttaca atcgtagcca aaccaccaaa aaagagatag
    25081 actagtatct cccaatatcc ttttgcttct aaatatcctt ttaactgtcg atactgcttc
    25141 atgagccaac ctaccttatt aaaaattctt atttatcata ctctatattt caataacttt
    25201 catctttatt taaaaaaatt aatttttttt gatcctttgc ctttgtctct gcttggatcg
    25261 tcacatgtgt aataccatat tttaatttta ataaagaaat tatacgagga tatatctttt
    25321 caatctcgct tagtagctga tcttcaaaat caatatgtcc gtcgaaaata attatctttt
    25381 cgctatactg ccaaatatgg acatgatgaa catcctttac cccatcgatt gcttcaattt
    25441 cttgttccat tttttcatag tcgatagcag gtgcactttg tatcaaaata gcggcagctt
    25501 ttttgatcac cattatcgct tctatcaata tatatatcga aatgatcagt gtaataactg
    25561 gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc tccgataatc acacttaccg
    25621 aagacagtgt atcacttaac aaatgcaaat aactagattt tatattcaaa ctttcatctg
    25681 cttcttgtcg taataataat actgagaaca aattcgcgag caatccaata actgctactg
    25741 ttagcatcaa atgactattt atcttttctg gtgaagtgaa ccgcttgaat gcttcaacgg
    25801 ttaaaaacaa ggataatatg atcaatatga atgagttgat gaaagcactc aatatttcta
    25861 aacgctggta gccatacgta cgttttttat ttgcttcttt tcgggccatt ttttgtgccc
    25921 cgtaagaaaa aataattgcc aatgtatcac taaggttatg gaaagcatct gaaacaagag
    25981 acaaactccc tgacataatc cctccaataa attctgataa agtgatcacc gcattcaaaa
    26041 ccactactcc aaatagtttt tttgatgatc tctgtggctg tgtaggcact attccttcct
    26101 ccattttatt tatattggat agtctttaga caagaataac atttttttga tatttcacca
    26161 atcattcagc tgtttcctta aagcacttgt atttcacttg atacttcgct agaattgcaa
    26221 gtgatgacac tactagatga ggagtactta tgacaacaaa acaattaatt atcagttcct
    26281 ttatacgatt caaagaccta aaggaggcga gaaatgtccc attttggaaa gccattgtct
    26341 atcttttagt tttgagtata attatggctt tgccaatctc ttttcaagtt tttcaagtct
    26401 taaacaacat tcaattggat gggcaaaaga ttgctactcg cattcctgat tttacaatcc
    26461 aaaacgggca gcttcaaacc gaagaaaaag aaggattcat ttaccaaaca aactccatta
    26521 tcttcacatt tgatccagag ggaaaacgaa cagaacagga catctctact gatttgatgg
    26581 ggaattttgt tagtgtcggg atgttaaaaa acaaacttat catagctctt cctaatacag
    26641 gcacgacttc tgctttgttg aacaataacc aattagaact tccctacacc aatgaatctt
    26701 taaaaaactt aacaggtaag cagctccgtt cttttttaag tgaagcatct ataccacttt
    26761 ggataaaagt cctgacattt atattttcta tttatcctag ttttctgaat ctgctgatca
    26821 ctttgctatt taccaacatt gctgcttttc tctatgcacg ctttcgacta acaaaagcta
    26881 cttttcttga ttgtttaaaa acattgatct attcaatttc attgcctact ataattgcaa
    26941 caatcttgat ggtctttctt ccttcttttg acactagcgc cttcattgct tttgcaggaa
    27001 tctttatttt tgctcaagct gtcaaaggat gggcaaaaat atcgatcaat taagaaataa
    27061 agacaactcg ttatttctac ttcaatagac ccttttagag gttgtgacat ttttttgtca
    27121 cagccccttt tttatttgaa aaatatttct ttctcaaaaa acaagcaagg cgcttcaaag
    27181 ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg gaaattaact atgtcagaaa
    27241 gaatcattta tgcagatcgt ccatttcgtg atgaatttat cgaagaaatc catactatag
    27301 cgcctggtta tatctttaaa acagaattac aaccagagga tctacaaaat gtagagatca
    27361 gtttgggctg gaactcagac taccaagaaa aactattagc ctcttctacc ttgaaatggg
    27421 tccaatctat ctcagctggt gttgatcagc taccccttga gactttttca aagaaacata
    27481 ttttactttc aaatggtagt ggcatccaca gtgaatcgat tacggagcat gtcttaggaa
    27541 tcatccttgg gtattctcgt ggtctttttc aagcacaaaa ggcacagagt gacatgaaat
    27601 ggttaggttc atcggtccat taccaatcta tccaagggaa aaaaatacta atcattggaa
    27661 ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc attagacatg aactgctatg
    27721 gaatcaatac atccggtcat ccagcagaag gaatggaaaa gactttttct ttaaatcaac
    27781 taaaggaagt tcttcctgaa gtagatttcg tagtcaatat cttacctcta acggaggaaa
    27841 caactgggct ctttgatcag catatgtttg aacactttga tccaaaatct gtattcatca
    27901 atgttggccg tggtgcttct gttaagacac aagatctggt gcaggcttta aataaccagc
    27961 aactttcctt tgcagcatta gacgtttttg aggaagagcc tcttccacaa gatcatcctc
    28021 tttggaaaat ggacaatgta ctgattactt cccacattgc aggtttgaca cctgattttc
    28081 aaaagaaatt aatggctatt tttttaacca atctgaaaag ctactttgcc acccatgagc
    28141 ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata gctgaaaaca atattttcaa
    28201 aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt gttttttaaa
    28261 gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat atcctttaaa
    28321 gaaccattca tctgcttcct cgataaaaaa aagtgtccca tccatttcca ctttctttac
    28381 aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt
    28441 gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa tttctgagtc
    28501 aaaccacttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct actttctctt
    28561 ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct ctccaataac
    28621 cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga taaccgtttg
    28681 atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc
    28741 taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag aatatttatc
    28801 tgacaactct aggtaatcta aaatattctt tgacactaaa ttatgcgcaa tccttattcc
    28861 catgtctcgc tcgggatgga cgacgcgatc agctccgatc ttttcaagta ctctggcatg
    28921 atactcattt tgggcttttg ctaaaatatt tggtacgccc atttccttta ccatcagagt
    28981 aactaagata ctggcttgaa tatcttctcc aatagcgaca attacatgat cgaaatttct
    29041 tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt gccacaacag catgagtggc
    29101 tatattcata tattcattga cccgatcttc attactatca atagccagta cttcttgacc
    29161 agcttcgatc agtgttcggc agatacttcc gccaaatcgt ccaagaccta caattgcaaa
    29221 attttgtttc accgttttcc tcctttagta tcttcttttc tcagtataac aaagccttta
    29281 caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata gttattttct
    29341 cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt ttgaatgcat
    29401 agatagcggc ttgtgtacgg tcttccactt ctagcttagc caatatattg gaaacatgtg
    29461 tttttactgt tttcagtgta ataaacaatt cgtccgctat ttcctgatta ctttttcctt
    29521 cagaaatcag cattaaaatt tcattttccc gattcgttag ttcttcatgt aatacaggct
    29581 catttcgccg actcattttt tccatcattt ttgttgtcac ttctggttct aatactcgct
    29641 cgccacgttg cgttgccctt atagcatcag caatttcatg tgcggtagag gtcttcaaca
    29701 aataccctgc tgcacctgct tcaatggcag gataaacttt ttcgtcatca ataaaactgg
    29761 ttacgataat gattttagct tctggccaat cttttaagat tgcttttgtt gattcgatac
    29821 catccatttc ttccatgact aagtccatta aaatgacgtc cggtcgaagt gtcatcgcct
    29881 tctcatagcc ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct tcttgaatag
    29941 ataaataaga agagacgcct aagcgcacca tttcatggtc atctactaat aaaactttta
    30001 tcatccttat ctatgcctcc ttcatcaaag ggaccttgat ttctacactt gtaccttgac
    30061 ctttaaaact aatgattttt actgttccac caataccagc tactcgttct ttgatattgt
    30121 tcaatccata acttcccgtt ttcgtttcat tcatattaaa ccctgttccg tcgtcaataa
    30181 tacgtaacaa aagattattg tctattttgt gcaaatatac ctctaattca ttggctttag
    30241 catgtcttaa tgtatttgat aacaattctt gaacgattcg gaacaagtga tcctcaatgg
    30301 aactagatag ttttacatct tctacatccc atttcagcga aatctggatt ttattttgca
    30361 attccttcaa taactgctcg ataccttgct ttaaactttt ctcttctaaa ttgactggac
    30421 gtagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc tctgctacca
    30481 ttttcaactg cttgcgaaac atttctggtg tctcgctttt ttctgcttgc tccgtcaatg
    30541 ccgacatcat catcattgct gcaaacaatt gttgcgaaac tgaatcatgc agctcacggg
    30601 ctaatcgatg tctttctaat tctaaaattt cttctttggt ttgtccatct acatattggg
    30661 gacggctcgt tacaatttgt aattcactag atacttcaat cattttttct tttatcttag
    30721 tgatttcctt atcaatatct ttgatgtata aatcgtccga tgcacttggg atcggaagaa
    30781 gcaacaattt ggattcataa ttgccagaag aaagtgcacg cagcttttct tcgattttcc
    30841 cgtattttgt cttttgaata agtgataata atagaaagac aattaatcct acacctaagg
    30901 aaatggctaa caaatggaag ataagcggaa catataataa acgtgcacgc agtaattctc
    30961 cccaccaatg actttgattg ctggcataaa aataagtgaa aagtgaaaat agaataataa
    31021 ggaaagccgc aatccccgaa taaacagcta gcatcgctct ggatattttt cccatcatat
    31081 tcgtatcacc tcgacatctc caatcaaggt attagtaata atcttcaatc tacgagggtt
    31141 ttcatcataa tcactgctgt agattttgat ttgttcattt ttcaaagaaa attgctcttc
    31201 ttcaaacaag acatttccta ctaatgtcgc atgctctaat cggatggcta cgcccaaagg
    31261 tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat catcttttgg
    31321 caaaagcgta ttgcccaaat ctatgatagt atcaccagaa atgacggcga tgttgatatc
    31381 gtcccattca tagacgtcgt ttccaatacg ttggttaccg aataattgct gtcttttccg
    31441 ctcattatta tgtgttttta attgctctgt ttggatcatc atgatctgtt tctttcgcca
    31501 aaaagcattt ttggttaaat ctatccctga aatctctaca cctttcagac caataaataa
    31561 aacggcaaat acagccatca tccataaagc aggactgttg actaagctaa aaaaaatgac
    31621 caaacttcct aatatcaatt gaaaattttg aaatttagta cgaggatgtt tgcgcatagc
    31681 tagatatata ttgaaaatac cgaatatcac taaaagcaaa agtccagtat tgtttacgat
    31741 ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc acaaaaaaac gccaagaact
    31801 attcatcgct ctttctccct cctgacacat tctcatttca tcatacctat ttttttaact
    31861 gttggctata ggactttaga tgtaagatcg tttgttgaaa tctaaaatac acaaagagaa
    31921 aaagatctag agtgtatcat cagtaagatg aactctctag atctttcagt cagactacgc
    31981 ttgttctact ttgacgattt taactaacat atcgccacct ggagtagcaa tagttacttc
    32041 atcattttta cgtttgccaa tcaatgcttg cgcaatagga gaatcattgg aaattttgcc
    32101 agaaaacgga tctgcttcag cgcttccgac aatcgtgtac tcttcttctt ctccatctgg
    32161 tagttcgatg aatgtaactg ttttaccaat tgatacctca tctgcatcta caccgtcgtt
    32221 atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt gtgattcttc cttcaacaaa
    32281 tgcttgttca tctttcgctg attcatattc agagttttcg gaaagatctc cgaaaccgcg
    32341 ggcaattttg attcgttcaa tgatttctcc gcgtttgact gtttttagtt cttctaattc
    32401 tttttctaat ttttctttac cttctagagt cattggataa actttctcta ccatgtgtaa
    32461 cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata tagtccaaaa
    32521 aagattacca cgcttttctt caaatgtaaa tgtttttcta aaaatgatca atgtctagag
    32581 cctgtttttc taagaattat ttacatattt ttccactaat tccatatgtt cttcataagt
    32641 tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat agtcattttc
    32701 tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag gtccaggtcc
    32761 atagccggta ttggtataca aattatatgg tgaatctaca gctgtatctt catacgtcac
    32821 taattctttg tgttcaccta atgcgtataa aacagagata tctgactgaa gtggcatatt
    32881 tgctttgatt cggttaaaga agacttgtgc aatgttttta cgatcatttt cttttacgcc
    32941 ttctttttca actagagaag ctagagttaa aacttcttgt acagtcaaat ttttctgctt
    33001 gatagctgaa taatagtttt gcatgaccgt attcgtcttg ttcaccattt caatcactag
    33061 atccttaagc gaagtactcg tgtaataatc atatgtggct gggaataagt aaccttccaa
    33121 acggtatttt gtgttctcag ctttagatgc actttccaac agttctggga atttttggtg
    33181 caactcgtta aagaaagtct catcattcac taaatctaaa aattctttct tgtctttacc
    33241 agttaccttc gctacccgtt cagcaatctg atcgatatcg tacccttctg gaatggcaat
    33301 tttcccatct gcaacttttg ttggctcaga agtaccgcct tcttgcaact gtttgccaat
    33361 ctcgtcaaga gtcatattag gcgctaactg ataatagcca gcttgaaagc cagttaaatt
    33421 tttaaatttt gtataataat tgaaaacaat cccgctttta ataatattgt ccttctccaa
    33481 aatctcacca atctgcttgt tagaagagcc actcgggatc tcgacttgga ctaattggtc
    33541 atctgattta tctaaaggct tcaacccgct atctacataa cgatacacgg taaatccaag
    33601 aaatccgcca ataatcaata atgtaagagc aatcactaaa atgatcttcc tcacaatctt
    33661 atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag ctgccttact
    33721 ggcttctttt ttatctttac ttgggtgttg tttgtttcct gacaaaacaa ctcctcctta
    33781 ctatcaagtt cgtaacttta ttcattatac atgatatttg aattggcgtg aatgatttta
    33841 tttaaatatt ctatcaaaaa tgtaagtagt aaatccatta ctataagatt aaaataaatg
    33901 ttatcaagat acaagctatt ttagctttat tctttctgta aaaaagaaag gagactaaat
    33961 aaactattat gaaaaaagga aaacatgcta aaaaaagaaa atattcattt ttaatgatcg
    34021 ttgtcgtgtt gtttagcctt agtgcgttct ctacaggaac tattcttgga tggcaatttt
    34081 acaataatga acactctcag atagttgccc atgctcaatc agctcaaaaa aagttgatac
    34141 tgactaccaa tgaacaacag ctcgttgaaa aaatcgatca actattaaga aaaaatgatt
    34201 atattggctc aatttacgta aagaaaaata atcgcatcat catggaaaaa ggctatggct
    34261 atgcgaataa actcacgcaa caaccaaatg atccttctct ttattatcag attggttcgg
    34321 ttcaaaaagc attgactact cttttgattt taaaacaagt cgagcgtggg cacctttctt
    34381 tagatactaa gctggctcaa ttctatccac aaattgctgg cagccagcaa attactatcc
    34441 gtgacttgct gtatatgcgc tcaggcttgc accgaactgc ttctccaacc ataccgatga
    34501 ctgatgaaga agtcgttcaa tttgcgattc agcacttaaa atttacagat tatcagacct
    34561 ctcattatga ttcattgaac tttaccatat tgactggaat tttgatcaaa ttaacaaatc
    34621 aaccgtatga aaaattattg accgatgata tcatcgaacc tttaggatta cagcatacaa
    34681 aattttatga ccaagtcaaa aattcatcca atcatgcact atcctatcaa atgtctaaag
    34741 aggatgatta ctttaaacca ctgacagaat ctgaaacaga gatccataac gaattaggga
    34801 ctggtaatat cagtatgtcc gtctatgatt taaatacgct gtttacacaa gttttatctg
    34861 gaaaactagt ttccaaagac ttactcttct cattatggcg tgaaaatgcg aatggacatc
    34921 catatagtgg tggggtatat agcggagata attatatcct gtcgcaagga aatatcgact
    34981 cttttgatac atctgctata tttagcaaag attcacagga tgctatcatc atggaaagca
    35041 atataaaagc tgacaaaact atcaaactgc ctactgctga tctaagaaac caaatctatg
    35101 acctgatgga aggaactact aagttggcaa atagctgata tgatgaaaag atagcaaata
    35161 ctacttcttt gaatagcatt tgcttaaaaa aagtactcga ctaaacgtag gaaagagtgt
    35221 gggacaaatc taaaaatcaa tttttgctac agactttaat tccaaataaa cagaacagaa
    35281 agaggtgttc ttctt
//
LOCUS       DACDLI010000021        34718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDLI010000021 DACDLI010000000
VERSION     DACDLI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.021"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..1861
                     /locus_tag="IV353_001093"
     CDS             155..1861
                     /locus_tag="IV353_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9009360.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="IV353_001094"
     CDS             complement(2019..2921)
                     /locus_tag="IV353_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9009361.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3622)
                     /locus_tag="IV353_001095"
     CDS             complement(2918..3622)
                     /locus_tag="IV353_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9009362.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="IV353_001096"
     CDS             complement(3653..4294)
                     /locus_tag="IV353_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9009363.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4646)
                     /locus_tag="IV353_001097"
     CDS             complement(4332..4646)
                     /locus_tag="IV353_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009364.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4634..5263)
                     /locus_tag="IV353_001098"
     CDS             complement(4634..5263)
                     /locus_tag="IV353_001098"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9009365.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="IV353_001099"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="IV353_001099"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9009366.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="IV353_001100"
     CDS             complement(5983..6915)
                     /locus_tag="IV353_001100"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9009367.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="IV353_001101"
     CDS             complement(6912..7688)
                     /locus_tag="IV353_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9009368.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="IV353_001102"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="IV353_001102"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9009369.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /gene="carA"
                     /locus_tag="IV353_001103"
     CDS             complement(10932..12008)
                     /gene="carA"
                     /locus_tag="IV353_001103"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9009370.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="IV353_001104"
     CDS             complement(12013..13293)
                     /locus_tag="IV353_001104"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9009371.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="IV353_001105"
     CDS             complement(13312..14238)
                     /locus_tag="IV353_001105"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9009372.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="IV353_001106"
     CDS             complement(14251..15534)
                     /locus_tag="IV353_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9009373.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="IV353_001107"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="IV353_001107"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9009374.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="IV353_001108"
     CDS             complement(16322..17233)
                     /locus_tag="IV353_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9009375.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /locus_tag="IV353_001109"
     CDS             complement(17230..17694)
                     /locus_tag="IV353_001109"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9009376.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="IV353_001110"
     CDS             complement(17699..18397)
                     /locus_tag="IV353_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9009377.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="IV353_001111"
     CDS             complement(18384..18770)
                     /locus_tag="IV353_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009378.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="IV353_001112"
     CDS             complement(18917..20587)
                     /locus_tag="IV353_001112"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9009379.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="IV353_001113"
     CDS             20770..21003
                     /locus_tag="IV353_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9009380.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="IV353_001114"
     CDS             complement(21102..21533)
                     /locus_tag="IV353_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9009381.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21612..22025
                     /locus_tag="IV353_001115"
     CDS             21612..22025
                     /locus_tag="IV353_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9009382.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22063..23211)
                     /locus_tag="IV353_001116"
     CDS             complement(22063..23211)
                     /locus_tag="IV353_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9009383.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23333..23929)
                     /locus_tag="IV353_001117"
     CDS             complement(23333..23929)
                     /locus_tag="IV353_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9009384.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="IV353_001118"
     CDS             complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="IV353_001118"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9009385.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27159..27590
                     /locus_tag="IV353_001119"
     CDS             27159..27590
                     /locus_tag="IV353_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9009386.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27635..28984)
                     /locus_tag="IV353_001120"
     CDS             complement(27635..28984)
                     /locus_tag="IV353_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9009387.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29054..30217)
                     /locus_tag="IV353_001121"
     CDS             complement(29054..30217)
                     /locus_tag="IV353_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009388.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30346..30669
                     /locus_tag="IV353_001122"
     CDS             30346..30669
                     /locus_tag="IV353_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009389.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30852..32201)
                     /locus_tag="IV353_001123"
     CDS             complement(30852..32201)
                     /locus_tag="IV353_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9009390.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32270..33223)
                     /locus_tag="IV353_001124"
     CDS             complement(32270..33223)
                     /locus_tag="IV353_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9009391.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33325..34515)
                     /locus_tag="IV353_001125"
     CDS             complement(33325..34515)
                     /locus_tag="IV353_001125"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9009392.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9868 a         7599 c         6094 g        11157 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaacaga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacga tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaaaaggag
    21601 aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac cctggtccta
    21661 gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct atcccgcttt
    21721 ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta gaatttctaa
    21781 agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa acactagttt
    21841 ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt caaagaatcc
    21901 aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa aacaaaaata
    21961 aaggagcaga taatcttgca cgtcaggctc tccataaaca attaaaagaa aaaaagactc
    22021 tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata aaagataatt
    22081 gtgatcaaac gtaatttcca attggtaatc tccgattttt tcttctattt gactacgtaa
    22141 gattctttct agttctgtat caacaaactg gaatttctca gggaccacta catcaaattg
    22201 cagcgttttt ccttgatgaa ttactctaaa atcatgaata ttaagccctg tacctgtttc
    22261 aagaagaatt tgctttaact gccgcaagat attatagtat ttttcgttat caacaggcat
    22321 tggatccaag tgacagacaa gttccacatc caaagacttt ttaaagtctt tttctatcag
    22381 atcaataatc ttatgagcca cattcaaatt cattgattca tctacttcga tgtggactga
    22441 tgcaaagcgt ttattgggtc catagttatg caccaataaa tcatgatagc caagtatatc
    22501 atcgcaagca tttaaatgct cttccatttc ttcaatctct gctggcgttg ggcgacttcc
    22561 taatagttcg tatacaaaat ttcgaagcat cgtcaatcca ctataaagga tatataatgc
    22621 taaagcaaaa cctatatatc catcaatacg ccatccagtt acccattcga ttgctgctga
    22681 aagcaataca gccagtgtag taaagacatc attataacta tctgttgctg tagctctcaa
    22741 tgtttcagaa tcaatggatt gcgcaataac ctgatacatt ttgccttgtg caaatttcaa
    22801 aaagatggaa atgattagga caaaaaagac gatgggtgtt aaagagacat tttctggatg
    22861 gatgattttt tcagcagaag actctaaaaa ttgaaatcct acataagtaa tgatgatcga
    22921 aacaaataac ccactgatat attcgaagcg ttcgtgtcca taaggatgtt cctgatctgc
    22981 tggctttgca gcaattttaa atccgatcaa agtcaatatg gatgaagcag tatctgatag
    23041 actgttcact gcatcagcca taatggagac acttcctgaa aaaagcccga tcattagctt
    23101 agagacaaac aagatcagat tcgtaaccaa tcctaagata cccgctagta ctcctttttt
    23161 tgtccgctgt tgcggtattt gtttcttttt ttcttcttgt ttcttagcca agttttttac
    23221 cccttctagt ttgcttgttg ctcaggtaaa aagatggaga ggacgattgt tttcctctcc
    23281 tctcttcctt tttctatcta tcgtgcgtcc tattactatt aaaagatggt cattattctg
    23341 gtttcaacgg tgttgctgca aataacgctt ttgctagtaa tggtgttatg attcccgcac
    23401 ctattgcttc tggtatccca ttgattgctg caacccctgc taaggtttta aataaaagag
    23461 cagagtctac accataggca tctgccgttg cacctgtata aaataatccc atgaatccta
    23521 acactaagac tgtattgacc aatgaaccca gtacccctgc aaaggctgag ctgatcgtta
    23581 ttgatttatt tttttgataa atccagtgga aaaccaagcc tgctactaag ccaacaagta
    23641 ctctgggaac taccgagata atcggattcg tgaacactaa tgtatcaaat ggcgttgttg
    23701 gcatggcata ggcacgaaca attgtagcga ttccccaaaa caagccaatc accatgccat
    23761 cttttgggcc taatactact gcagcaataa ttactgtgat atggataatt gttaaactaa
    23821 tgaatcctaa tggaataaac cccagccaag gaaccatcgc ttgtacgatg atgattgcta
    23881 acaagatcgc acgtaagact agtcgaaagg tcttatttct actattcatt ttttcacccc
    23941 tttttattta tactaatcgt aaaaatttct gtcactttcg tcaactaaag accatccgaa
    24001 tccgaccatt tgattagatg cgccatccaa ttgaaaaaag tatgagggca agacttgttt
    24061 gtcttcgccc ccataatcat gtttcgttca atgtttgaac ttttgttttt ctgtcaaacg
    24121 taaacgccct attgttgcgc aacccattct ttcgcagctt taagtgcatc tggaatacca
    24181 gcaggatttt tcccgccagc ttgtgccatg tctggacgtc caccaccgcc accgcctact
    24241 ttcggtgcga ttgctttaat caagtcgcca gcttttagtc cagcttcatt tgattctttc
    24301 gtcattgccg ctagcagact gacttttcct tcttgttcgt ttgctaaaac aagaatatct
    24361 gaaatatttt tttgtttcca ttggtcagct aattgacgca gctgattcat atctttcact
    24421 tttacttgtg ctgcaatgat tgtttttcca ttgatcgttt ctggatcttt gaatacatca
    24481 ccagcttgtt gattcgctaa cttgctagct aattgttcat tttccttttg tagctctctt
    24541 acttgctgtt gcaattgttc cgcttttgaa acaacttctt tcaattgagg tgatttaaca
    24601 attgttgcta cttgttttag ttgtttttct tcttcttcaa gtagttcgta cgcttcttta
    24661 cttgttacag cctcgattcg tcgcacgcct gcaccaattc ctgattcaga aacgattttg
    24721 aagattccga tgttttctgt atttgctaca tgtgtgcctc cgcacagttc gattgaccag
    24781 ccgccaatat ttactactcg aacgtcatgt ccgtattttt caccaaacaa agccattgca
    24841 cccatgtttt tggctgtatc gatatctgtt tcaattgttt caactgggat agcttcccag
    24901 attttttcgt tgacgattct ttccatttca gctaattcat ctgaagtgat ttgaccaaag
    24961 tgggtaaagt cgaatcgtaa atgtccagga gcgactaaag aaccagcttg atttgcatgc
    25021 tcgccaagta cttctttcaa tgccttgtgc agtagatggg ttgctgtatg gtttttgatg
    25081 atccgattgc gcatcgattg atcgatcaat agatgatagg tttgtccttc actcattggt
    25141 gcttttactt ctacggtgtg cataaattga ccattaggcg ctttttgaac atctgtaaca
    25201 actgctacga tttcgcctac ttcattcatg atccatcctt gatccgctac ttgcccaccc
    25261 atttctgcat agaaaggtgt ttgatcaaag atcatttgag cttcaccttc tgcgacttca
    25321 ttcaaaagag actcaccttt aacgatgacc aataattgac tatctgattc ttgttctgtg
    25381 tatccaatga atttactttc tactttgata tctgtcaata aagcagattg tacccccatt
    25441 gatttttcag tactgcgggc agagcgagca cgttcgcgtt gtgcatccat ttctttttca
    25501 aaaccagcat gatcaacttt cagtccttca tcttctgcca cctcttccgt taattctacc
    25561 gggaagccat atgtgtcgta tagtttgaag atgtcttttc ctgctagagt cgtcccgttt
    25621 tcgtttttta cttcttggat cacttgattt aagatttcca aaccttcatt gattgtttca
    25681 ttgaaacgtt cttcttctgt acggataact ttttcaataa attctttttg ttgaagtact
    25741 tcaggataat aacttaccat gatttcccca acaacaggga ctaaacgata taagaatgct
    25801 tgttcaatac ctaatttttt cccatgcatg acagcacgac gaagcaaacg gcgtaagaca
    25861 taccctctcc cttcattcga tggcaatgca ccgtcgccaa tagcaaatga aagggcgcga
    25921 atatgatctg cgattacttt gtaagaaata tctgttactg ggttttcacc atattttact
    25981 tgtttgctta gcttttctac ggcgtggata ataggcataa acaagtctgt ctcaaagttc
    26041 gttgacgcat cttgaacgat tgagaccatg cgttccaatc ccatccccgt atcaatgttt
    26101 ttatgaggaa gtggttcaaa tgtgttatca ggcttatggt tgaattctga aaatactaag
    26161 ttccagattt ctaaataacg ttcattttct cctcctggat agttttcagg atcgtcttct
    26221 gctaaatcgt tataagcttc tccacggtca tagaaaattt ccgtatccgg accacatggg
    26281 cctgcaccga tatcccagaa gttgtcttct acatcaataa tgtgatctag tggcaggcca
    26341 acttcttcat gccagatgcg ttttgcttct gtatcttttg gataaactgt tacataaagt
    26401 ttttctggat cgaatgccat ccattttgga tcagtcaaaa attcccatgc ccagtggatg
    26461 gcttcatttt tgaaatagtc accaatcgaa aaattaccca gcatctcaaa catcgtatgg
    26521 tgccgtgctg ttttaccgac attttcgata tcatttgttc gaatagattt ttgggcattt
    26581 gtgattcttg gattttctgg cacaactgaa ccatcaaaat attttttcaa tgttgcaaca
    26641 ccagagttga tccacaataa ggttggatcg ttcacgggga tcaaagacgc actaggttca
    26701 atcgtatgtc ctttagattg gaagaaatca agaaacattt gtcgaacttg tgcactactt
    26761 aattgtttca tgtcattacc tgctttctat aaattaaata caaaaaaaca ccatcacagc
    26821 caaggacgaa aactcgcggt accaccttga ttgcaatgat gtgtaatcat tacctcttaa
    26881 actcgataac gctgagctgc gtcggtattt ctaccaaaat agaaaaaggg agcaatttga
    26941 ttagtgacta ttcttttttc agccaagaaa gaattttctg tgagtcttaa aaaacaaatc
    27001 atatcctttt cgtacttaat tagtatacgc attctattga tcactgtcaa tatctcaatt
    27061 tttattttca tttccttttg gtcatactcc acgatttttg gtacgataag aataaaggaa
    27121 tcaatacatc tctgctattc atgacaagga ggaatatgat gaaaaaaata gttggagcgg
    27181 atgaatggat acgcgcagct agctgctttt tgagatatca agtttttgta ttagaacaag
    27241 ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc tattatctat
    27301 ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca cctacttgct
    27361 tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta ggcggtcagt
    27421 tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt tatgtagtag
    27481 ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc acagaacctt
    27541 ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa aaacaagaga
    27601 ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt ttcttatttt
    27661 tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta tttttttctg
    27721 caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt ttcttcttca
    27781 caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg gcacgtctat
    27841 tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca aatgagacgc
    27901 ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac gtaatggccg
    27961 ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag tctagatctt
    28021 ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct ctagcagcta
    28081 aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc actcgttttc
    28141 tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg tttttcaagt
    28201 aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct aaataaggat
    28261 gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt gttgaaatca
    28321 gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca atgattggat
    28381 tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca gaaaaaacta
    28441 aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt aaaaaaccca
    28501 tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga atcttcagcg
    28561 cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc tgtggctgtt
    28621 ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa ttcgagacac
    28681 ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc tgtgcagcta
    28741 gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca ttgatttttt
    28801 caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat tgcccaatga
    28861 tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg ggctgtttga
    28921 agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata tcaaaattat
    28981 tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac acagacaaag
    29041 cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc gtgctaacag
    29101 tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa aatgatagca
    29161 agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg aatactgcac
    29221 tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac tgccccaacc
    29281 agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat gcttttaaag
    29341 aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg ccaagtgtcc
    29401 atccattggt tataaacggt acaatcataa agtatacccc tacttctaat aaaagccacc
    29461 agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc gccgctacca
    29521 aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt cgagtcaatg
    29581 agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta ggaagcataa
    29641 atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt gtcactaaat
    29701 cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca cttagctgcg
    29761 tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata aaagaaagcc
    29821 ggaaataata tcttaaataa atgccaaatg gtatgagtaa taaaacatta aaagctggtt
    29881 ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac aagaccaata
    29941 ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc tttggccctg
    30001 tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa tacgcacaca
    30061 ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat gttccatact
    30121 ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat ggaaaaacaa
    30181 tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt gattctttgt
    30241 atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct attatttctc
    30301 atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct gaatatgtac
    30361 tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc ttatgtaaga
    30421 aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg tttcatgcta
    30481 gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat gactccacat
    30541 gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat tttcatttct
    30601 ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca gttacgcaaa
    30661 tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt gctatttcat
    30721 cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca tttcttccac
    30781 gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct aataaaattc
    30841 gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc aagtgtcaat
    30901 actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac gataaaagtc
    30961 cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta tggtcgaaac
    31021 aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt ataaaaaatc
    31081 caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat catcatgcca
    31141 atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt atcatgaagt
    31201 ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg caataacata agggagacgc
    31261 attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat accaactgtg
    31321 accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc cgcattgctg
    31381 tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac taataaataa
    31441 acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt cgccggcttt
    31501 ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa catcgttgct
    31561 agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg gatcggaaac
    31621 agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac aaacaaagga
    31681 ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga acctaaaaaa
    31741 ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat tgactgggaa
    31801 agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc tacatttgct
    31861 ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt tccgtaaatc
    31921 cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag ttcagctcct
    31981 gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat atgcaaactg
    32041 gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc accgatcact
    32101 gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa ttcattttct
    32161 atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc cctttcatac
    32221 catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt tataccgtct
    32281 ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct aagtcgtatt
    32341 tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt ggccaagatt
    32401 ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct ggtatgattt
    32461 tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata ttgaaggcat
    32521 ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca tcgatcgcat
    32581 ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg tactttcctt
    32641 ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct cctggcaagg
    32701 ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca tatttcacat
    32761 aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg gttgtaggac
    32821 gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac gcgccgattg
    32881 aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca ttgaccagcc
    32941 cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct gacaaaagag
    33001 cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc attttttcgt
    33061 aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca ggcatgcgta
    33121 tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg atcaactctg
    33181 aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc ctccctgatc
    33241 ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc tctaatttta
    33301 aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta ttttttgata
    33361 aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca tatttctgat
    33421 cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa tcccatgttc
    33481 gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga ctggagtaat
    33541 aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt taaaataatt
    33601 agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag taatcgaagc
    33661 caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg gccgacttct
    33721 agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa caacacctat
    33781 agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa aatgcttgac
    33841 ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt aggtgatcag
    33901 tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc catccatgga
    33961 gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta tcgcttcctt
    34021 tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt ttgctccgga
    34081 taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga tagcatcttc
    34141 ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt gaaaggcgat
    34201 tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat ggattttcag
    34261 cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg ttgcttcaat
    34321 acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg atgctagatt
    34381 gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta gtatgtcgat
    34441 cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat acattgttag
    34501 ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac ataccaaaca
    34561 tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg cttacactga
    34621 ttatgagaat tatttaacca cattttttat ttacagtatc tttttacttt caaatgactt
    34681 atatagtatg aacaattacc ttttagataa tgatctat
//
LOCUS       DACDLI010000022        34640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDLI010000022 DACDLI010000000
VERSION     DACDLI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.022"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..549
                     /locus_tag="IV353_001126"
     CDS             <1..549
                     /locus_tag="IV353_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9009393.1"
                     /translation="SSKKTIKQISTETGINYTTLTNYYRGERNPRNTETWNILADYFH
                     VPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIASLDSDYFTELSNLYFSMTS
                     LLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKALNLNIDERKELESELFGN
                     LTQVQVNNLKREITARLISKLQ"
     gene            604..1770
                     /locus_tag="IV353_001127"
     CDS             604..1770
                     /locus_tag="IV353_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9009394.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(1859..2251)
                     /gene="rpsI"
                     /locus_tag="IV353_001128"
     CDS             complement(1859..2251)
                     /gene="rpsI"
                     /locus_tag="IV353_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9009395.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2267..2710)
                     /gene="rplM"
                     /locus_tag="IV353_001129"
     CDS             complement(2267..2710)
                     /gene="rplM"
                     /locus_tag="IV353_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9009396.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3229..3696)
                     /locus_tag="IV353_001130"
     CDS             complement(3229..3696)
                     /locus_tag="IV353_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9009397.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3816..5354)
                     /locus_tag="IV353_001131"
     CDS             complement(3816..5354)
                     /locus_tag="IV353_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9009398.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5434..6120
                     /locus_tag="IV353_001132"
     CDS             5434..6120
                     /locus_tag="IV353_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9009399.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6321..6848)
                     /gene="msrA"
                     /locus_tag="IV353_001133"
     CDS             complement(6321..6848)
                     /gene="msrA"
                     /locus_tag="IV353_001133"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9009400.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6932..7609)
                     /gene="pgmB"
                     /locus_tag="IV353_001134"
     CDS             complement(6932..7609)
                     /gene="pgmB"
                     /locus_tag="IV353_001134"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9009401.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7606..8736)
                     /locus_tag="IV353_001135"
     CDS             complement(7606..8736)
                     /locus_tag="IV353_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9009402.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8736..10919)
                     /locus_tag="IV353_001136"
     CDS             complement(8736..10919)
                     /locus_tag="IV353_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9009403.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10916..11941)
                     /locus_tag="IV353_001137"
     CDS             complement(10916..11941)
                     /locus_tag="IV353_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9009404.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11986..12813)
                     /locus_tag="IV353_001138"
     CDS             complement(11986..12813)
                     /locus_tag="IV353_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9009405.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12810..13877)
                     /locus_tag="IV353_001139"
     CDS             complement(12810..13877)
                     /locus_tag="IV353_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9009406.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13903..14739)
                     /locus_tag="IV353_001140"
     CDS             complement(13903..14739)
                     /locus_tag="IV353_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9009407.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14736..15605)
                     /locus_tag="IV353_001141"
     CDS             complement(14736..15605)
                     /locus_tag="IV353_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9009408.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15675..16958)
                     /locus_tag="IV353_001142"
     CDS             complement(15675..16958)
                     /locus_tag="IV353_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9009409.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16972..18657)
                     /locus_tag="IV353_001143"
     CDS             complement(16972..18657)
                     /locus_tag="IV353_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9009410.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18932..19957
                     /locus_tag="IV353_001144"
     CDS             18932..19957
                     /locus_tag="IV353_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9009411.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20552..24205)
                     /gene="rpoC"
                     /locus_tag="IV353_001145"
     CDS             complement(20552..24205)
                     /gene="rpoC"
                     /locus_tag="IV353_001145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9009412.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24284..27901)
                     /gene="rpoB"
                     /locus_tag="IV353_001146"
     CDS             complement(24284..27901)
                     /gene="rpoB"
                     /locus_tag="IV353_001146"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9009413.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28629..29597
                     /locus_tag="IV353_001147"
     CDS             28629..29597
                     /locus_tag="IV353_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9009414.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29615..30520
                     /locus_tag="IV353_001148"
     CDS             29615..30520
                     /locus_tag="IV353_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009415.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30517..31323
                     /locus_tag="IV353_001149"
     CDS             30517..31323
                     /locus_tag="IV353_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9009416.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31480..32448
                     /locus_tag="IV353_001150"
     CDS             31480..32448
                     /locus_tag="IV353_001150"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9009417.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32765..33289)
                     /locus_tag="IV353_001151"
     CDS             complement(32765..33289)
                     /locus_tag="IV353_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9009418.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33576..>34640
                     /gene="scm"
                     /locus_tag="IV353_001152"
     CDS             33576..>34640
                     /gene="scm"
                     /locus_tag="IV353_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9009419.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         9905 a         7375 c         6481 g        10879 t
ORIGIN      
        1 tccagtaaaa agaccattaa acaaattagt acagaaactg gtattaacta tacaactctt
       61 acgaactatt accgtggtga gcgaaatcct agaaatactg aaacatggaa tatccttgct
      121 gattatttcc atgttcctgt aagttatttg atgggattag acgaaacaaa ttccatagaa
      181 caacctaatc ttgatagaat aaaaactgct cgtttagaaa aaaaggacaa aatagtcaag
      241 aatattgcca gtcttgactc agattatttt actgaattat ctaatctata cttttccatg
      301 acttctttac ttgaagtcaa acttcaagaa gatgactccg actttctttt tttaaaaagt
      361 attctagagg actcctttga atacacgatt ctatttgcca aacaaaagaa gtttattaat
      421 aaagctttaa acttaaatat cgatgaaaga aaagagttag aatctgagtt atttggcaac
      481 ttaacccaag tacaggttaa caatctaaaa agagagataa cagctcgatt aatatcaaaa
      541 cttcaataaa actaattatt caatcagcca actcgccaag ttatcagatt ggagagatta
      601 gttatgtcaa tacaaaagta cacaacaaaa agcggtgcac gatatagtgt ccgcttgtat
      661 gttcgtaaag atgaaaatgg aaagcaagaa tactatgtaa aaaaagggtt taaaactgaa
      721 aaagaagcca agttacacga agcaagaaaa aaggtcgaga ttgaagatac cggctatcat
      781 aagccttcta atgacttgtt tataacagtg tatgaagaat ggttgccttc ttataggaat
      841 cgtgtacaag aaacgacata tcaacgtaca aaagaccttt tccgcttgca tatactacca
      901 aaattcggga ataagtccat ttctaagatt actcctgttc actgtcagaa tgcagtcaat
      961 gaatgggctt taaaattcaa gaatatcaaa cagcttaagt cttacagctc tcaaatattt
     1021 gagtatgcca tttttgcaga gctattacac cgaaatccta tggcaaatac aaatctacca
     1081 aagcgggaaa agaaagaaaa tgaaaattac ttttcattag aagaactaaa agctttttgg
     1141 gatattctca ataaagaaga gcctttaaaa catattttaa tcttccagct tttgattact
     1201 actggtatac gtaaaggaga gctagctgct cttcattggt ctgacattta ttttgaagaa
     1261 cagctaatgt atgttagaaa gagctatgca actattcgaa atgaagagaa gaatgcaaga
     1321 agaaaaactg tcaggattca aaaaaataca aaaaatatat cttccgaacg aatattacca
     1381 attgatgcac aaactattga aatgttaaaa atctggaaaa aagaacaagc tcgagagctt
     1441 ttgcagtttg gcataaatac aaatacaaaa aatcaactta tctttaccta tgtaaatgca
     1501 gacggtgaca ttaatcaacc tttgcatgcg gactattcaa ataatatatt gaaacgttta
     1561 gagaagaaat ataaatttaa gcacgttact attcatggat tccggcacac tcacgctact
     1621 cttctactag aaggtggagc aagtatcaaa gaaacacaag atcgattggg tcataaaaat
     1681 gctgaaacta cccttaacac ttattctcat gttactgaaa aagctcaacg aaatgcgtta
     1741 gaaaaattca ttgcttacac tggtttataa tattgtcatt acctttctca ttaccagaac
     1801 gaaaaaaaga caccttctcg caatgagaaa gtgtctgaaa tcgttggtat aacaacgatt
     1861 aacgttttga aaactgtgaa gctttacgtg ctttcttaag acctggtttt ttacgttcga
     1921 ccatacgtgc gtcacgagta agtaagccag cacgtttaag agctaaacgg aagtcaggat
     1981 caacttctag caatgcacga gcgataccat gacggatagc tcctgattgt ccagcgtatc
     2041 ctccgccgtt tacgttaaca aatacgtcat atgcgccttt tgtttcagta acagcgaaag
     2101 gttgattgat tacttcacgc aagtcagcat gtggaatgta ttcttcaaca tcttttttat
     2161 ttacagtaat tttgccagtt cctggtacta gacgtacgcg agcaactgcg tttttacgac
     2221 ggcctgtgcc gatatattga acttgtgcca atgaaattcc ctcctattag attaagtttg
     2281 tgatgtctaa aacttctggt tgttgtgctg catgtggatg ttctgctcca ccgtatacgt
     2341 tcaattttgt aaattgtttg cggcctaaag tgtttttagg aagcatacct tttacagaag
     2401 tttcgatcaa acgacgagaa tttttagcac gcaattcgcc agcagtgatt gatttcaatc
     2461 ctcctgggta gttgctgtga cggtaataaa ttttgtcagt cgctttttta cctgttaatt
     2521 tcactttgtc tgcattgatg acgatgacga aatcgccagt atccaaatga ggtgtgaaag
     2581 ttggtttatt tttaccacgt agtacagatg caacaactgt tgaaagacgt cccaatggaa
     2641 catcagttgc gtctactaca taccatttac gttctacttc gcctggtttg gccatatatg
     2701 ttgtacgcac gttattttcc tccaatttaa gttctgatgt ttgacataca cttcgagttt
     2761 ccggggctct ttgtggggca aacaatacca tttaacatat tacccgctaa tctagtcaat
     2821 gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac tttacttctt ttctttcttt
     2881 tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct
     2941 tgtcacaacc ccttattttg aataaacggt gttctcctgc caactcctcg gtcacaagtc
     3001 acaatatcca taaaacataa gaagctgttt tttaaaattg tttcttgtga ctcggagctg
     3061 aacggcaggc ttacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc
     3121 tcaaaaaacg gcaaaatatg aggaactatt ttgacgtttg tttctctaat actcaggtca
     3181 aaacgctgat ttcacaacct ctttttcggt caagttcaac aacagttttt acgcgtcgat
     3241 ctctggcgct ttgcccaaat gagcttggat catccagata cgtttttctg tggctgtttt
     3301 gaattcgata aagatatctt gtgttggatc gtcgccttct tctccagaaa cttcgattcc
     3361 tttttggtaa agatctgcta gataacgata gcctgcaaca agtgtttcca aacgttctgg
     3421 aatagtacga tcccagcggc caatttcatc agggattttt gtgttatctg caaattcacg
     3481 caatgtagaa taaggtgaac cgtctaatgt gatcaagcgt tcggatactt catctaactg
     3541 agcgttcaaa tcatccatga actcatccat cattgggtgc aatgtaagga aacctggtcc
     3601 tctcatgtac caatgtgttt ggtgaacaac aacagacatt tggcttagat ctgcaactaa
     3661 ttgatttaat acttctttgg tttgttggaa tttcatcttt cttgcctcct aacattttct
     3721 tacacttaaa ataatagtag actttcaatg aaaagtacaa caaaatcatt tacctgagaa
     3781 cgattttcag ttcgcttttt tatccgtaaa gccaatcatt tttcagaatc agctaattct
     3841 tctcgcagtt gagcaaattg ctgataagtc tgtgctaaat gcttttgtgt cgaaacaaat
     3901 aatggataga ttttctgata ttttttatga tcagtttctt gtactccaac ttgctctttc
     3961 aattctatca attcctgtgc ttcactgata ctttggatct ttccgatgct tctccagcct
     4021 aaaatcacag ctcctaatgc ggatgcttct gtgacagtag ggaaaacaag cgggtaaccc
     4081 agtacatcag ccatcatctg acgaaaaatc ggagattgag caaacccgcc tgtagcacgg
     4141 atctctgtga gctgattttc ctggcaaact ccttttaaaa tccgataaag gttgagacag
     4201 atcccttcta gaatcgcctt catcatttct ttttctgtat gttgccgctg taaaccgata
     4261 aaactgccaa acgcatctgc gtcccaaagc ggtgctcgct caccaagtaa atagggatga
     4321 aaaatcagac cattggcacc cgctggcaca aaagcgatag ccgaaagcag tgcatcatat
     4381 ggattttctc ctttttcttt agcagattga acgacttcct gtaaaaatgt atgccctgcc
     4441 caatcaaaga tgccagcacc attgctagtc gctcctccca ctacccaatg tttctgatcc
     4501 agaacataac aaaacgtctc catttctgga tggaactgag gctgagtagt aataaaacga
     4561 agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat caccaaaagc acctaaccct
     4621 agattagaaa gcggcccgtc tgcccctccc aaaacaaatg atatatcatt tggcaaacct
     4681 agttctgctg ctgtttttga tggtagacct gttacttgat aggtggaagg aaagacttgc
     4741 ggcaattgct ctattcgaat gttcaagtag tcaagcactt cttctgccca ttggaattca
     4801 tggatatcaa aatatccagt tcctgaagct gttccgtagt cgctgatcag ttcgccaaac
     4861 aatctataaa agatatagtc tttgataccg acaaaataag cagttttttt gaataattca
     4921 ggttggctgt tttgcagcca cttgattttg ttcaatggtg tcattggatg aatcggcgtc
     4981 ccagtctgcc gataaaattc tttgccaagc acggtatatt tcagtttttc tgcttcgtcc
     5041 gctgctcggt tatctgccca agtgatcatt cgagataacg gtgttttctc ttcatccaac
     5101 aatatcaggc catgcatcgc agaggagaag gagacaaatt gaatgattgt gtctgaaggc
     5161 aactgtttaa ttacttcttt tataacggtg cagacacctt gaaagatcaa ttcctgatct
     5221 tgttctgcca tcccttgtgt ttcttggatc aaagggtagc gaaaataagc ggatgctgct
     5281 tcttgggctt catctgtgaa agcaactgct ttggtttgtg tggttccgat atctaatccg
     5341 attgaaacgt tcattttttc acctcaaatt ttatgtatgt acaaaaattt agtttaagct
     5401 tgcgtatctt ggttttgaaa agggggagat ggcgtgttac tttattttat cattggttgc
     5461 tgtatcggat catttttatg tttaaccgcc cagcggattc cgttaggtca ctctattatt
     5521 tatcctagat ctcattgtgt caaatgctgt cgttcgcttt cttggtatga attgattccg
     5581 attctctcga tcatcgtcca acgatttcgc tgccgctatt gcaggtgtcg gttgcccttc
     5641 tactatttgt tggcagaagc cttatgtggt ggtctttttg cctggttctt tactttttct
     5701 tccgcaagaa atttttcgac gttcatatgg atgctttcgg ctttgctatt ttcgttgatg
     5761 gatttatttt acttcatggt ccattctcac acgctattct gttcttgggc aattttatgg
     5821 attttttggt tgcaggccgg cgtcttccaa tggcaaagtg tgctgttaac gtttatcgta
     5881 ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag ctggcgatac tctgttgatg
     5941 ctttcttgga gtggtggctt gtctctagaa gagttgcttc agatcttgtt tcttgctagt
     6001 ctgtttggaa tatccgtttt ttctgtttac tttatctgtc ataaaaaaaa gttggaaaag
     6061 ctgcctttca ttcctttttt gagcagtgcg ttgctgattg tccttcatct taggtattga
     6121 aaaaaggttg tgacaaacga cttgtcacaa cctctgatat atagtgttct cttaccaaat
     6181 ccttgtgact tggagctagg cgacaggttc acaacctctc cactaaatgg tgttcaatca
     6241 gctgaccctc agtattagct caaaatgctg gttttacaac ctctgatata cggtgttcca
     6301 taagtagctt tttcggcttc ttatttcttg acgccccaat gtcgacgaag gaaacctgca
     6361 cgtccagaac cttctcgata aagattgtaa tggagcttgt ttttcttgta ataatcctga
     6421 tggtaatctt ctgcaggata aaattcgctt cttggttcga tttttgtcac gattggctta
     6481 tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct ctgcaatatt tcgttcttct
     6541 tcatcattat agtagatcac tggacgataa gaatcaccgc gatcagcgaa ctgtcctaat
     6601 gcgtcagtcg gatcggtttg ctgccaatag atttccacca actgatcata agaaataact
     6661 tctggatcat attcgatctc tactgcttct gtatgccctg ttgtgcctgt tgttacttgt
     6721 tcataagttg gatttggtac atgcccgcca gtataaccgg atgtcacaga aatgatccct
     6781 ggctgctctt caaatggttt gaccatacac caaaagcatc cgcctgcgaa aactgctttt
     6841 tttgtcatat aaaatccccc ttttttctca tcataccttg atttccaaaa ggaggataac
     6901 gttttgcttg tgacagaaaa aacggaaact attatatacg tgatacggct tctgataaat
     6961 gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg aatcacatat tcagcagaag
     7021 gaagatttat gttttcacct attcccaata catgcatgtt accagccaaa gctgcttgac
     7081 aaccagcttc tgaatcttca atgaccaagc atgcatttgg cggtacattc aattgctgtg
     7141 ctcctttgag aaaaacttct ggatcaggtt ttgcttttga tacttgcgtg ccgtcaataa
     7201 ggacatcaaa ataacttgtc aaattcgttt tttctaaaat gaggcgtgca tttttgcttg
     7261 ctgatcccaa tccgatcttg atgtttgttt ttttcaagta ctcaagaaca gctattgcac
     7321 caggcaaaac ggatgtttca tccatttttt gaatcgcttg aagatataga ttgttttttt
     7381 gctcagcaaa ggctgatttc tctttttctg agaatttctg ttccattttt ccaaatgaca
     7441 gcaatcgctc tagtgactcg gttctggata ttcctttcag ttgctcgttt tctttctcag
     7501 tgaatggaat ggacagctgg ttagctaaaa ccagccacgc ttcgtaatga taacgagctg
     7561 tgtctacaag aacgccatct aaatcgaata gtgctgcttt gattttcatc gattacctcc
     7621 ttgaaaatga acgacttgaa gtaaagatgc gatgttttta gctgtgcgat gaagattatc
     7681 agtggcttgc tgtaaagctt cttgtaaagg cacagctgca ggaaggatcg gaaaaacggc
     7741 cgaaatgcca acatctgctg ctttttcgct tcctttacca acacttccgc aaatagctat
     7801 cacaggaacg ccctctggta ctcttctagc aatcccaact ggcgcttttc cttttagact
     7861 ttgtccatct agacgtcctt cgcctacgat tacgaggtct gcttgctgta cccgtttatc
     7921 gaactccagt aaatccaata cgtagtcgat gccagagact attttccctg aagcaaatgc
     7981 taacagtcct gcacccattc caccaccagc actactgccc gatgcctcga ttacttgagg
     8041 ggcaaatctc atataaaaat catgcatggc ttggtcagcc actgctaatt cattttcttt
     8101 caagcctttt tgtggtccga aaacaaaaga agcgccttct tttccgcata aaagattagt
     8161 aacatcagta atgacagtga ttttggcttt aatcagctca ataggcacgt tcgttttatc
     8221 aactgtagca attttaccta gattttctcc cagtgcttct actttttgat tatcttgatc
     8281 gtaaaaagca taacctagac cttctgccat gccgatgccg ccatcgttgg tactacttcc
     8341 gccaacgccg atgatgatgt gtcgaacacc ttgttgcaac aattgtcgta tcagttctcc
     8401 tactcctcgc gtggataacc taagtggctg ttgttggttt agtgggacat ctttcaatcc
     8461 gacgatttct gccatttcaa aaatcgcagt ttgaccgtct gaagcgtact ggatttcttt
     8521 gtttttgcca aatgcattag taacgactgc tgtttgccgc ttcaacctta aactgtctgc
     8581 taatgatgaa accgttcctt ctcctccatc gcctataggt agcagatcat attcagcctg
     8641 aacaaaaact tcttgaaaac ctgattgaat ggcttgtgct gcctctaagg ctgtcagact
     8701 ttctttgaaa gaatctggcg caatcagaat acgcattatt ttcccctccg ttccaagatc
     8761 agttcttttt ccagctggta ttggtctcca ttgacaaaaa taggtaatgt ttctttcgtc
     8821 tcttttttta ttactatttg tttttttgtc aaaacaaata gcaacctgtg atttttccaa
     8881 ataaaaggaa aagaaatttt ttcccatgct gaaggaagtc ttggagaaat agaaagagtt
     8941 ccattcgtta ctgtaatacc tgaaaaacca aatataactg ctagccatag tgctcctaat
     9001 gaagcagcat gaattccgtc atctgatgaa tgcggatctg gtcctaaatc aatcaggcac
     9061 gcttcttgga ataaatgata cgcccattcc atttcttgga tacggcaagc ctctatggcg
     9121 tgaatcgctt tactcaacga tgagtcgtgg attgttttgt tttcatagta atgcaaattc
     9181 ttagaaacga ttggttgaga aaaatactca ggtaataagt ataacagcat gacaagatct
     9241 gcttgcttgc tgacttggag ttcatttact tcgctacggg cataatcaag taaaatagac
     9301 tggctgccag gacgttcttt gtaagtggtc aaatcaataa ctggcttact gagatacgtg
     9361 tcatcttgcg gaatcagctg ttctttgtta ggcactggca aatataactt atctaaaaaa
     9421 ttttgacatt ctttcaaaaa aagcggatcg ccagcttgat atttaacagc taaggccaca
     9481 ttataatgcg ccagatagtt cgtatatgca ttattgtcga tatattctgt gtattcatct
     9541 ggaccaatga ctctgtgtat ctctaaatga tcatcgactt ttacagctct gctcagccaa
     9601 aaagctgctg tttcatgaat gagcgttttt ccctttgttt ctaaaaacga ttgatcctct
     9661 gttgcttgga catactcttg aacggcataa gcaaggtctg cactgatatg atgttctgcg
     9721 gcagctgaag caacttcttg tcttgttccg gttttgatat tcaatgcagc aaatttgggt
     9781 gtctcttcct gaccagtgtg tgcgctttcc catgggaaaa gtgcaccttg atagtgataa
     9841 tctcttgctt tttgccgtgc gccttctaat cgatcataac ggtacagcag caaattcttt
     9901 gctacttcag gaaactgata taaaaagaaa ggcaggataa aaatttccgt atcccagaaa
     9961 acatgccctt tataaccttc gccagttagt cccttcgccc ctacactaac acttggatca
    10021 ttgcggggag tcataatctg taaatggtaa gcagcgaagt ccatcgctaa ctgatcgaaa
    10081 gaagaatcgc cttggatcgt tatccgatgc tgatgccaaa aatctgtcca ataagacatc
    10141 gactgctcta aaatttcagt ataactaacg cttttctggg cttgggcaat acttttctta
    10201 aacgggtctg tttgctgaag agaggtagag agcacgatga tcttctccag cgtaaaggac
    10261 gtattttttc tgatcttttc tgaaaaggaa gcggtgatcc ggcgattttt tgcttggaat
    10321 acacctgctt tattgaattt agctgcaatc ccaatatcaa ttttactttc tgttgtctgg
    10381 taggatgcac tgatcaaatc gtcaccaaat actcgcagtt ctttttcaac caactgctgt
    10441 gtaccaaaat tggtttgctg tccatcgata cccgttttca catctacttg gcagtcttcg
    10501 gtcaacggct tgatgaccac tttcagggct gcctgatgga gattattttg atcagcaaca
    10561 cgacaagtaa tgacttccac tttttttcct tgagcagttt caatgagcgc atagcgttta
    10621 agctctccca tttctaaatc tagtgcccgc tcgtatgcaa gaactttcat cgtctcaaga
    10681 gaaaaatgtt caccgtcgat ttttatttcc catctgatga catctgggag attcactaac
    10741 tcggctgatt ctgctcctaa aggtcggtta tatacaccag ctgcaaacat tccccttact
    10801 tgattccgat agtcttcctc ctggcttcct ctgataccaa aaaatccatt tccaatggtc
    10861 atctgactcg catacttatt gatatactct aaatcaaact gtttttcttc tactttcata
    10921 aggtaataac ttctcctgtt tctgccgaac gatataaatt ttccaccaga ttctggatga
    10981 catatccttg ccagccatct gcaatcatct gagtctggtc accgagcgcc cgattcacaa
    11041 aatgagccat acttagttca tgtgggtcat taactggcgt ggtcttttgt aaggaaacca
    11101 gctttccatc tacatcttcg aatagttcgc ctggatacag attagcacct gcctgatcgc
    11161 cacaaaaagc cacattcagt ttgtcttctc tgatattcaa agcaaacgaa gtattcagtt
    11221 ggatgatctt gccgttttct aattccagtg tgccaaaaat tgcatcttct actgtgaatt
    11281 tgcttggatc ccatttcccg aatttccctg aatttttttt cgtaccaatt ttttggaagc
    11341 tgtacgcccg aacgcttttt actttaggaa atccagtaat atacatcgct gtatcaagca
    11401 tgtgaatgcc ataatcgatc aaaggcccgc ctccttgaag ttctttgtcg ataaacgttc
    11461 cccagcctgg aacaccagac cgacgaagcg cattcgcttc tacgtaataa atatccccga
    11521 gaacatcaag atgattcttg atcattgttg cttcgtcaga aaaacgatgc tggaatccat
    11581 aagccaaaat ctgcccgttt ttatcggctg tctctgccat ctttcttgct tgatcagctg
    11641 tcatagctgg tggtttctcg cacatcactg aagcattagc ttcgagggca gccaatacat
    11701 gttcgaagtg aaagcgattg ggtgtacaga cactcacgat atcgggagaa atctcattca
    11761 gcatttgtgt aactgatgta tacgcatgag aaaatcctgt acgtcggcaa aagtcttctg
    11821 ctcgctggct gactggatca accacagcgg ccaattctaa gacatctgat cttttttgat
    11881 aataagcagc atgtactttt tctgctactt gtccagcgcc tatgattgct actttcttca
    11941 tttatttctc tctcctttgc ttatttactt attgttattc tttctctatt ctttctctaa
    12001 atcatttaaa tgacgcgctg tttccagaca gtttttcaag tattgcaatg attcctgata
    12061 aattttcact tcattttctc ctcgcaaacg acattcatat accatataac cttcatactc
    12121 attttgaagt agcgtttgaa aatggcgagt aaaatcaatt gcgccagatc ctggttggta
    12181 gcgatgatta tctgctaaat ggatatgacc cacatactct ttttgcgtag tcaatgcttt
    12241 tgaaatatca tcttcttcaa tattcatatg ataaaaatca gcgatgatct gcacatgttt
    12301 caattgatta gagtcaatgt aatgacgcgc atccgctaac aaattgatca tatggtcttg
    12361 gtatcggttc aaaggctcca aataaataac cgtacctgtt tcttcggcta ctttatctaa
    12421 ataacgaagt gattcgctga ctgctttata gtctccttct ttgcttcggg gtgaaaccat
    12481 cggcggtaat cgataagtga acattcccca agctgctggg ataacgattc ctttgcctcc
    12541 aacctcgctt agtgcacgca aaatctcagc aatttgacgt acaccattaa gacgccgttc
    12601 ttcgataaag tcaccgatcc acccatcgta gccgttacaa gctgtcacta ctgggatccc
    12661 tgtttctttg attgcctgtt tgactgtgtc gatattttct attaacaact taccgtcgat
    12721 ttcgtattct tcaaatccac agtctttgat aaatctaaat ttttctaaaa tgtctcttgg
    12781 gaaaaatgct tgatcttgcg taccgaattt cattgttctc ctcctcggac ttgaacgccc
    12841 attttgatac tttcttctgg gtgctggtct acataacgca tgtaagcttc tgcgctttct
    12901 gcaaatggca caactggatc aatgatttct tcacaatcca agtaaccgtt catcaataat
    12961 tcccaacatg tgtcctcgat tcgtttacgg ttccatcttg ggtaatctgg gtttggttca
    13021 ctagatgctc gagcaaagac gatttttgca ttattgaaat gtgcttcgcg accaagatta
    13081 aacccagctg gaaaaggttt agcaaaggca acataagaaa tgatcccgcc atagccgatc
    13141 cctattaaag aagcttgtaa tgcggcagca ctgccactcg tttcaataac tgaatctact
    13201 ccacgtttat ctgtcaattc ctttagacga aaaccgacat ctgctttcat aggatctagc
    13261 gtgtaatcgg caccgtgttt ttctgctaat tctctacgct gcgctatggg atcgatccca
    13321 acgatggtag tagcacctgc ttttttagct aattgaatcg caatttgccc gatagcgcct
    13381 aaaccgaaga ttgctacagc atcacctgct cgaacatgtg cttcccggat accacctaaa
    13441 gcgaattgag caggatcgta acatactgca tttttccatt gatccaaccg atcaagttta
    13501 cgtagtttat aattatcaac accgtttgta atcagcgttt cacgaatggg tccataagtg
    13561 cagactaact ctcctttttg atactctgtc accaaactgc ccgtttcttc gatcgtaccg
    13621 acaatcatat ttcctaattg aaattcgcca aataccacac cgcgttggct tccttcttct
    13681 cttggcaaga ataggcgcca ttcttctgaa aattcttcat cgataaatgg acttaatcct
    13741 ctaaagtcga caatttctgt tcctggtttt ggagtagcga aaattactcg gatacgtact
    13801 tcgttctttt cgatctcacg gtcttcgtaa tctttgaatc cggctattct tggttctaat
    13861 gcaactaatt ttttcatctt ttctttcctc tttcttttct ttttatcctt ttacgccgcc
    13921 ttcgactaaa tcacctttaa taaatcgttc agataacgca tacataatga tgactggtaa
    13981 agcggtcacc aatgaagcgg ccatcattcg tccccaaatg tagtctggtg tactgaataa
    14041 tgtgttcaaa ccgattggta aagtaaattt ttcggaggtc gacaggaaaa tcgaagcaaa
    14101 taaataatca ttccacgaaa ccataaagca ataaacgaag acagaaatga ttccagaaat
    14161 agccaatggt ataatgatcc gaataatgat ctggatccgg ctaagaccat cgatcattgc
    14221 tgcttcttca atatcagtcg gaatcgtgtc aaaataactt tttagcatga atatcgcagt
    14281 aggaagtgtt tgaacgacca tcgtgataat cagtgctgta ttggtatcat acaaacctag
    14341 accggaaatg attttaaata atggaacaac gatcaaaata ccggaaaaca tataaacggt
    14401 gtaaaagcta gcattgattt tcgagcgagc tttgaagcgt agttttgaca aagcgtatgc
    14461 cccgctgatc ccaatcacta ctgcagttac ggaggctatc aaggagatca ctaaactatt
    14521 tttgaaatac atgatgaatg gaaaaatctc agcattgaaa atatcctgat aatgttcaaa
    14581 agtccattct ttcggaaaaa tagtttgtgt cgttgcaatt gcttcttgtg aatccttgaa
    14641 tgatgtcatc agcatgatca aaaatggaaa caaattgaca gctaatagca acaaaatcgt
    14701 cgcgtaaaat aagattttcc tgctcttttt atttttcatt tcaagtccac cttctttcgt
    14761 gagagcaaga cgaccgctgc aataatgatg aacaatagaa tcgatatagc tgctgctttt
    14821 ccaagatcat tgaacgcaaa ggctgtctga tacaaataaa cgcctaagat attgacttta
    14881 ttcgtcagta gatagacatc tgtaaacata tagaacatcc agatagatcg taaaacaatc
    14941 acagttgcta gtactggttt gattgccggc agtgtcacga tcttgaactt ctgccaagca
    15001 tttgcaccgt ccatttcagc tgcttcataa agcgttttgt cgactgtttg caaaatagcc
    15061 aaaaatgaaa taaacgcata cggatagtat ctccagatag caaacaaaac gaccataaag
    15121 aagctagcaa ctggctggtc gaaccacagc ggtgcttcgt tgaaaagctg caacctatct
    15181 actaacagat cattgacgat tccataacta ttattgaaca tatatttcca agcaaaaatc
    15241 aaagaaatag atggcgtaac ataacttaat aaaatcagtg aacgcaccgt tttcctaaat
    15301 ctgaacttgc ggttaagcaa aatagctacg gctaatccta aagctgtact gcctgctaca
    15361 actaagaatg tgtaagcaac tgttaaacct aaagaacgat aaaattcact atcacttaaa
    15421 atttcaagat aattgcttaa tccgataaaa acggcgtctg agttaggatt cagcggcata
    15481 tcgaaaaaac tgatctgaat attggaaagc attggaatca gcactaaaac agctaataaa
    15541 agcatgcttg gtgctaaaag tcccatggct aattttaagt cagaatgttt ccttggacga
    15601 ttcattgaaa cctccccctg ataatctgcc aatagctatc tacttttttc gaacaccatt
    15661 ggcagaactt cttgttattt cgtatttttt tgtgcgtcag ctaaagcttg atcgacatct
    15721 tcgtttccga cagttacttg gttcactgct tttccgatca cacctgagct tgtgatatct
    15781 cccattttcg tgaaattctt ttccccgact agtccaaaga cttgtacttc atcaaaagca
    15841 ctagcaatct cagatggcag ctcgccaaaa gacttgatga cttcattttc tttgtaacca
    15901 tccagttcga ctacttgttt attcactggt tgagcaccac caggagacat caagacccac
    15961 ttttccatgt ttttcggttg agataagaaa gctgtgaatg cttcagcagc ctctttttgt
    16021 ttctcgtcta gaccagaaga aatcgtcaat ccagatactg tgccatagac agcttgatct
    16081 ttttgtgttg ggatagcaaa accgatattt tctgagttcc ccgcttcata aacagaaggc
    16141 aaaatgtaag ttgaatagat tgccattggt gctgttccat tcatgaaagc atctttgatt
    16201 tctgtcacat cattagagcc tggcatcgtg tattgggata agttttgata aaacgacaat
    16261 gcttctctca tcttttccgt atcgatcgtt acctcgcctt catcatccaa aacatttgct
    16321 ccatttgaca aagcaaattg agagaacgct tgctcactca ttgttccttc agcagtaggc
    16381 atagcaattc catattgttt attctcttta tctgtgaaat gttgagcaat ttttaaaata
    16441 tcatcccaat tttctggttc tgaaaaacca gcttctgata atttttcttt gtcataccaa
    16501 ataccttgaa cccagccgct aattggtgca gcaatatagc tttttccatc ttctgaacga
    16561 acaaggtttt tggctccgtc ataataatta tcttctccag cttcctcgat aacgttttgg
    16621 actgccgtct gatcaatcag ttcatcttta tccatcactt tagcaaaatc ctgactgact
    16681 tccattactt ctggtaattt accggaacga gctaacgtaa cgatttttgt attgaacgct
    16741 gattcttcta ccggcacttg cttgattttg atgtctggat tttctttttc aaaatcagca
    16801 accagttcat tgatcacact taatcgttcc tgttcgacag aagagtgcat gaattgaatc
    16861 gttgtctgtt gtgattcgtt agagctttga ctttcaccac aagcagtaag aagaaatcca
    16921 gtagctagca gacaggtagt aacaataatt tttttcattt ttttccacct cttattttat
    16981 tattttcagc cacatatatt catacggctg gaatatcaac tttcgttctt gactgcaatg
    17041 gataatttgt ccagaaaaca ggtctaggta atctcctttt ggtaaaagat aacctgctga
    17101 ttgattggaa aaattatgga taactaagac tttctcttcc ttcgcttcgc gaataaaact
    17161 gataaaagaa tctgctgagt ccagtaagta aatcaaacta ttcggatgga aggctttttc
    17221 tttttttcga atagatagca tctgggtcaa ttcaccatat actgctgttc ttaatggatc
    17281 tgtctgcaac tctttctcaa tatctgctaa caaaaatttt tgccgattga tttttcgatt
    17341 ctctcctgtt ttttctacgc ctttgtaatc atttcgactt cccagcacac tttgaatata
    17401 aattgccggc acgcctggta acgttaccaa aaatgaatga gcagctaaaa agcgtttgct
    17461 tctccattcc gtcgtatctg ttggagaact taacgcatct agatacgtag cgtttatctc
    17521 atatggactg gtcgttccat ctgggttttt cttgtagttt actcgggcac cctctttctt
    17581 cagctgttcc actaattcaa ggatcgtttt ttccggcaga atacctcgta ccggattcaa
    17641 tccgataccg tcatgggagg ctaaaaaatt aaaaaaagtt gtttttttcg atgcgaaatc
    17701 gatggttttt aaccattctt tcaacttact tgtattaccc gaacgaatcg cttgaagggt
    17761 caaaggtggt agcgaaaact gatagaccat ctgtgcttca ttctcgccat tcccaaaata
    17821 actgatatta tctttgtgtg gcacatttgt ctcagtgatc aataccgttc ctggagctgc
    17881 ttcatcagcg attgcacgga ataattggat gattttatgc gtttctggca ggtgtataca
    17941 agaagttcct agttctttcc ataaaaatcc cacagcgtcc aagcgaataa atgaagcgcc
    18001 tttttctaag taaaacaaaa gaatatcgat cattttcaat aaaacttctg gatttttaaa
    18061 attcaagtct acttgatctt cgctaaaagt cgtccatacg tgtttttctt tcccgttttt
    18121 gtcaatgaac gttgtaagta acgggctggt ccttggtcta gtcacgctgc ttgtatctgt
    18181 atccagtggc aaatcaataa aaaaatcttt gtacgtatca ttcaagttta aatattcctg
    18241 aaaccaagta ctttcggcag acatatgatt gcagacaaag tcgaacatta accgtgtttc
    18301 gcttctcatt tcttctatat cttcccatgt gcccaaacgg tcgttgattg cctgataatt
    18361 tacaacggag aaaccatcat cagatgtata tgggaaaaat ggcaagaaat gtacaactct
    18421 aaatgtatct gccaaatact tttgataaaa tttcttaaat gtacgcaaag ttggttgctg
    18481 ctcttcaaaa aattgatccc cataagtaat caagaaaata tcttcttcgt cccaatgcag
    18541 tttgcctgcc tggcctttct tctgctgata aacactgact ttttgttcaa tcatagaggc
    18601 ggttttctca gacaaatctg agtcgcgata aatcgcttgt aaaagttttg tatacatcgg
    18661 attccccttt cttttttggg agcgttccca acgatttgat catattatgg aaaacgctta
    18721 ctgtcaatag cattttttta aataattttg atatacacaa aaatacaaat aagaaagctt
    18781 atgtatcaat aattaatagc ttcttaaaaa aatatggtct ctcatgatca tgcaagctat
    18841 tcctaaattt tcaaaatcat agacaaaaaa gaatgtctat tgtactatgg aattattcta
    18901 aaacgctccc aaaaaggagg tgcatataaa attgaaacct actatttatg acattgcgaa
    18961 aaaagctaat gtctcaaaat caactgtctc cagagtatta aataaccagc caaatatctc
    19021 tgataaagca aaagcaagtg tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa
    19081 aattgcaaga ggactgtcag gaaatggttt tgacgcgatt ctagcattat ctcatcgtac
    19141 ttctcatacg acctatggca atcctttttt ttcagatgtt attcaatcca tttctgccgt
    19201 ttctgaacaa aataacttcg acttgattct acaaacggca aaaacaccag aagaagagct
    19261 agaaaaatgc gcgaaaaaga tccaagaaaa aatgatccga ggaattatta ttttaagctc
    19321 tacgatggat gaaacacttc tttttgaatt ggatcaatac aatattccta ttgtggtaat
    19381 cggaaaagtc ggaaacgact acacgaacat ctactcagtg gacacggata atttccagga
    19441 tagttacgat ttggtggaaa cactgatcaa gaatggacac cgtcatatcg gctgtctcca
    19501 tgctccgaca gaagtttatg tatccaatga tcgtctcaat ggttaccgtc aatgcttatt
    19561 tgatcaccac ctagatatcc gtaatgaatg gattatagac ggcggctttc gcttagaaga
    19621 tagcatggca gcaatgaatg atttaatgaa gctacgagaa agaccaactg cgattttttc
    19681 taccgatgca ttaaaatcat tgagtattta caaagtggca gaacaatctg gaaagcagat
    19741 acctgatgat ctttcgatcg caggattcag tgatccctta ttgtcctccc tattttcgcc
    19801 acagttgact cggatagaca ttccgacaaa tcagttagga atcaaagcca cagaactttt
    19861 attccaacta atccaccacc aataccctga agaaaaaaga atcattcttt caaccgagat
    19921 tactctcaca gattcgatcc aacaacaaga aatgtagaac agaattataa aagaacagaa
    19981 acactgagga taagtcagtt actctgttct tttttcaatc actcatccct cgttaccccc
    20041 tcccggtatt ttagcgaatt tttcttattt tccatctgtt ttagatagct gtggataact
    20101 ataatgtttg tgttaaacta ttttttccac agggaagaaa aaactatttt taaccacgtt
    20161 gtgtataagt ttttatagtt cgttttttta aacacattta cccttatttc aatccatgtt
    20221 atttggggat acttatctct aaaacataaa tagaataaca gacatgtcta ctggttttgt
    20281 tttattcgag gaattattca ataatagcta gcaaaatatt aaaaataacc gctctatctt
    20341 cccatcaatt tattttgcat tcaaaaaatg atctatcttt acttttgttg attgacttct
    20401 cttggaagat gatcgttccg ataccttaat tggatacgaa ccgatgttgt taagatcctt
    20461 tcccactttt cttctagtta gacaaattat tttagacaaa ccaaaaaaga ggttgtgaca
    20521 ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc attgcgtctt
    20581 ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt gctacaccga
    20641 cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg atgatcttac
    20701 ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat
    20761 ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta tttgtttcta
    20821 atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg cctcctgaga
    20881 ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta cctggtaaga
    20941 tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga accattactt
    21001 cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt acttcacgaa
    21061 gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt ggatcgattg
    21121 atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact ttcatacgtg
    21181 ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt acttctttgg
    21241 tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct gtgatcactg
    21301 cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga ccttgagtga
    21361 tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga gtaccaggtt
    21421 caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg tcagagccag
    21481 ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta catgtgaaga
    21541 cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg atgtcttcgg
    21601 aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt acttctttac
    21661 gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt ccctcgcgga
    21721 tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg attacatctt
    21781 gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat
    21841 cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg gatagtcctt
    21901 cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg gccatcaaac
    21961 cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca gaatcagaca
    22021 tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg atgctgtctt
    22081 ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg atcagaccac
    22141 gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg atttcttgtt
    22201 tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt
    22261 tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact ttgaatcgtt
    22321 tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat actaattctt
    22381 gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg tccggtgttt
    22441 ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga ggcatgatct
    22501 cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc cattcagtaa
    22561 atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa tgaacatatc
    22621 cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct tcgccttcgc
    22681 gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttga gaaggagtaa
    22741 caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc aacatacgtg
    22801 cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct ccatcgaaat
    22861 cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa
    22921 ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc agcaatactg
    22981 ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga cgttcgattt
    23041 tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca cgcatcacga
    23101 atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac atcttcaaga
    23161 aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct agcaagtttt
    23221 gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat gggcggttac
    23281 ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct actgcttctt
    23341 gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct aacaaacgtt
    23401 tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca cttgttgcaa
    23461 aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca gggataacgt
    23521 ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa atatccaaac
    23581 ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct ttcaattcgg
    23641 ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct gcacccatag
    23701 cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt tctgtcaaca
    23761 attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa gcaaaataaa
    23821 tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt gaaggaatcc
    23881 ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc atgcgttcac
    23941 ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt cctttataac
    24001 ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca aaaatccgct
    24061 cacagaacaa cccttcgcgt tcaggtttta aggtacgata gttaatcgtc tcaggttttt
    24121 ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct atttgcatac
    24181 tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa tggcaaaccg
    24241 caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct aaattatctt
    24301 ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt tcagcagctt
    24361 tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga
    24421 tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct tcgatatcca
    24481 agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg aatgattccg
    24541 gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc
    24601 cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag
    24661 cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga gctttacctc
    24721 ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc aatttgtcat
    24781 cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt ccatcaaatg
    24841 gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata ccggcttcac
    24901 gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt gctacgtgga
    24961 tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg atgttcatac
    25021 gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca ggtaggaatg
    25081 gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga cgaccggcca
    25141 ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact aacatgttta
    25201 cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg tgaacgatac
    25261 cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa
    25321 agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt ggcgtgactt
    25381 tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata cccatttcgt
    25441 ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt tcttcaggtc
    25501 ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa gtgtatacgt
    25561 catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac ccttcccaag
    25621 tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc atagatggtc
    25681 cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt ggacgttggt
    25741 tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta tctaatgcac
    25801 cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca ccgtcatgtt
    25861 tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca gtaccaaccc
    25921 atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca cccatcaagg
    25981 cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg gcaactactt
    26041 gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag ttttcacttt
    26101 gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat gggctgtttg
    26161 cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa
    26221 cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg aatttattta
    26281 ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca ggagtttcga
    26341 tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa ccggcgcggt
    26401 cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa
    26461 gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat tctttgatac
    26521 ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca gtatcttgga
    26581 tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg aattggtttt
    26641 gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata tcatcgacat
    26701 tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg tcggctggac
    26761 gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact tcacgttcag
    26821 ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg accacaccgt
    26881 cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga gctagtgttt
    26941 ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct gtttctggat
    27001 caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct aattttttgt
    27061 ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag aaacgagcat
    27121 tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa
    27181 cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag tctttttcga
    27241 ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa
    27301 atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg atacgtacat
    27361 aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta gggataacag
    27421 tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag acacctggtg
    27481 aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat gttccttgtt
    27541 ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc
    27601 ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca tgcgcacgag
    27661 cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc aaagatagat
    27721 tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta
    27781 agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc aacacttcac
    27841 tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg tgtccagcca
    27901 agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg ttacaataat
    27961 atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag gcaaaaaaaa
    28021 accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt tgtttcctta
    28081 tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga tagtttttgc
    28141 aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt ttcttctgct
    28201 ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg acaattctta
    28261 attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg aggaacttct
    28321 atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta ctttagctac
    28381 tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt ttgtatctat
    28441 gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc taagtggctg
    28501 agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt attttccgac
    28561 ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa attaaggagg
    28621 aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta ctagctttag
    28681 gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa acaattggcg
    28741 tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc aaagaaggat
    28801 tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa aatgcccaaa
    28861 acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat cctgatttgc
    28921 tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc actatcccga
    28981 tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc aatgaaaaac
    29041 ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa atcggcttac
    29101 tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa
    29161 actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca
    29221 aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt ctagctaaag
    29281 atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca agtgttgtcg
    29341 gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat caagtcaaag
    29401 acggcggtct agcaacagta ggaatcgact acgaaagctt gggcaaacaa acagggatca
    29461 tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg
    29521 atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat ccagacagca
    29581 tcaaagaagc tgaataaaac acaggaggat tctcatgaca aatttgctct ctcttttctt
    29641 atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat acgtcacttt
    29701 ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag gtgcagggat
    29761 cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc taggcttttt
    29821 agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga aaattcccgc
    29881 ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc gtgtgatggg
    29941 tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg agtcattcgg
    30001 cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga tcgttgtatg
    30061 tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa ctggcgataa
    30121 tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca tcggttatat
    30181 gatttctaat ggatgcattg ctctttctgg ctccttactt gcgcaaaaca acggctttgc
    30241 tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta tcatcgcaga
    30301 agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt taggtgcggt
    30361 catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa
    30421 acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc agcaaaaatt
    30481 ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt aacattggaa
    30541 aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt
    30601 aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa
    30661 tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca aattttattg
    30721 aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta
    30781 ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt
    30841 gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca
    30901 tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt atcctcagaa
    30961 atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc cacattacag
    31021 cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc tacttccaaa
    31081 acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc ttttatggtc
    31141 actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct gcatcaagga
    31201 aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc ccaattatta
    31261 gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact tttatcttca
    31321 taatcagaaa gaggatggga caaaaaatga acgcccaccc tcttcttttt tctctttcct
    31381 tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg aaaactattt tgttctctcc
    31441 ttcttcctga tagaatggat gggaaaggag cttgatgcca tgacaacgaa agacaaggta
    31501 cttcaattat tgaaaaagac accagatttt ctttccggag aaaagttggc acagcaactg
    31561 gaagtttccc ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt
    31621 tttgagcacg ggcctacagg gtatcgttac ctgccctctg acgtactaga tgcaaaagaa
    31681 atccagcaag agctacggtc gtttattcct gatttgaaag tacaaacgat gttgacttct
    31741 gaatctacga tgaaagatgc caaactggca gcagctcaag gagattctgt ttcgaagtta
    31801 tttgttgctg atacacaaga acaagctcgt ggacgttttg gacggccttt tttcgctgaa
    31861 cctggacgag gaatctacat gagtctcttg ctccatccag acaaacggtt tgatgaattg
    31921 cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt
    31981 cgttctgctt cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa agtctgtggg
    32041 attttatctg aagcagtcag tgatatggaa agcggcagga tcagtcatat catcattggg
    32101 atcgggctta atttttctgt tcctcaaaat cattttccag ctgatctcca agaaaaagcc
    32161 atctctgtct ttcccgacgg acaagctagt gtcacaagga atcagctaat cagcaaaatc
    32221 tggcaagaat tgttcactta tctttctcac ccagaaaaag cgttggactt ctataaagaa
    32281 aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt
    32341 cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg
    32401 agtttgtctt ctggcgaaat cagtctcaca tctatccgta cgcaataatt atcagatttc
    32461 acaaaaggac acctgatctc atacagtgtc cttttttctg aattctcttc aaaagactct
    32521 ccttattcta tcagctgaca gtttctctat cctctagaaa aaggtattct aaaaatatct
    32581 ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt
    32641 tacacgatca aaagagatag caggccgctt cctttttcaa tcttatagat agaccatggg
    32701 taacgataaa aacaaaaaag aacttgcgac ttattttgcc gtaagttctt ttttagctag
    32761 tgccttaatc tcttaatccc aacaaacgtc tcgcatagat cacctgacca gagtgcatag
    32821 cagcatcatc aataatagaa acgagacgca ttcctcgtgt gactggtggt gtccagttct
    32881 cgtcaattac ttcatctagc tcggaaggtt ccagccttct taagtaagaa atagcaaaat
    32941 cagcagctcc tcgcaagtat cctagcagat cttctatttg attagcttgg atctgctttg
    33001 cttgttccag actatgcttc caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc
    33061 tttcccagtc ttgactatgc caaattggag aagttcctgc taaatcagca atctggaaat
    33121 ctaattctcg agcggtatgc caagccagcc aagtcattga tttgatctgc ggggctctat
    33181 cttccactgg aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc
    33241 gatcgtatgc ccgatccaca ctctcgcata gcatatctaa ataatccatt tattttcctc
    33301 ctttttattt atcctactta ctttttccta attctaggat acctacttat atgttactac
    33361 acacccttat tttttacact ttaacagtca ccatcatcaa tagtttggag attgaaagaa
    33421 aaagatagtg tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt tcattaatat
    33481 tctttaaata aacaatgatt tttcaaacga acagtttaag actaggagaa aaagagtata
    33541 ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca agtttactag
    33601 tcctagttgc tttgttgttc aatatctttt cgtatggtgt ggccgtttca gcggaaacat
    33661 acattcctaa agaccctggg aatatttata atgatctaac tggtaactat ggcttgttag
    33721 gaattgctag tcaattccac gtatttgcta aaaataagac aactgtgaat gcacatacag
    33781 acggaaatat agcaacacat gaacttgatg caaaaaataa ttttggtaca gagcttcatg
    33841 aaggtttatt gaaccaagaa ataaattata tacaaaaaac tgattctttc gctaattctt
    33901 caggcatacc tacaggtagc acaagaatga ataaatttgt cgttggcagt aacgtggatg
    33961 tagcacttca agatggtcat cctacagtca atggatccag gcttgatcac ttaactccag
    34021 atgattttta taaagatcgt gctgggcatg aatatattga tttcgagcag gaatttgcta
    34081 aattgaatgt agcagcaaat gatcttgcaa cgatcactcc agctaaaaca tatactgctg
    34141 cagattttcc agacatgaac aatcggacaa tcgatttaac tgatttatca gataccgggt
    34201 ttttattggt aaatatcgat gcagaagtgt taacgatgaa tacgccactt caaatcatta
    34261 atccaaatga tcaagtagtt gtatttaatg tcatcaacag ttcttctgca ttgaacgtac
    34321 agtcaccaat caaatacaat gatcgttcaa atcatgaaac agaagatttt tcggatgcga
    34381 atatctcttg gaattttgga aatgagatga ctgatttgac aatcagtgct ccttttcaag
    34441 gaacaatcct tgcgcctaat gctactatcc gcattactca aaaccaagac ggaacgatta
    34501 taggaacaaa tgtgatttta gatgcagcta ccaatcgttg ggatcctaac gaaatattta
    34561 tcagcgacaa tggtacagat acgacagaca caagcgacag tacagataca tctaccacaa
    34621 gtgatagctc agattcatca
//
LOCUS       DACDLI010000023        34450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDLI010000023 DACDLI010000000
VERSION     DACDLI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.023"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(259..996)
                     /locus_tag="IV353_001153"
     CDS             complement(259..996)
                     /locus_tag="IV353_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9009420.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(998..2500)
                     /locus_tag="IV353_001154"
     CDS             complement(998..2500)
                     /locus_tag="IV353_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9009421.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2759..4753
                     /gene="uvrB"
                     /locus_tag="IV353_001155"
     CDS             2759..4753
                     /gene="uvrB"
                     /locus_tag="IV353_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9009422.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            4763..7579
                     /gene="uvrA"
                     /locus_tag="IV353_001156"
     CDS             4763..7579
                     /gene="uvrA"
                     /locus_tag="IV353_001156"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9009423.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            7842..9107
                     /locus_tag="IV353_001157"
     CDS             7842..9107
                     /locus_tag="IV353_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9009424.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9111..9842
                     /locus_tag="IV353_001158"
     CDS             9111..9842
                     /locus_tag="IV353_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9009425.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            9977..10861
                     /gene="rapZ"
                     /locus_tag="IV353_001159"
     CDS             9977..10861
                     /gene="rapZ"
                     /locus_tag="IV353_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9009426.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            10858..11856
                     /locus_tag="IV353_001160"
     CDS             10858..11856
                     /locus_tag="IV353_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9009427.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            11890..12825
                     /gene="whiA"
                     /locus_tag="IV353_001161"
     CDS             11890..12825
                     /gene="whiA"
                     /locus_tag="IV353_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9009428.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            13393..14049
                     /locus_tag="IV353_001162"
     CDS             13393..14049
                     /locus_tag="IV353_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009429.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            14228..14793
                     /locus_tag="IV353_001163"
                     /pseudo
     CDS             14228..14793
                     /locus_tag="IV353_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15492..16013)
                     /locus_tag="IV353_001164"
     CDS             complement(15492..16013)
                     /locus_tag="IV353_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9009430.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            16211..19891
                     /gene="nifJ"
                     /locus_tag="IV353_001165"
     CDS             16211..19891
                     /gene="nifJ"
                     /locus_tag="IV353_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9009431.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALNSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            20080..20967
                     /locus_tag="IV353_001166"
     CDS             20080..20967
                     /locus_tag="IV353_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9009432.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            20964..22133
                     /locus_tag="IV353_001167"
     CDS             20964..22133
                     /locus_tag="IV353_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009433.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            22157..23512
                     /locus_tag="IV353_001168"
     CDS             22157..23512
                     /locus_tag="IV353_001168"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9009434.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="IV353_001169"
     CDS             complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="IV353_001169"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9009435.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            25799..26089
                     /locus_tag="IV353_001170"
     CDS             25799..26089
                     /locus_tag="IV353_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009436.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            26102..27127
                     /gene="sppA"
                     /locus_tag="IV353_001171"
     CDS             26102..27127
                     /gene="sppA"
                     /locus_tag="IV353_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9009437.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            27130..27789
                     /locus_tag="IV353_001172"
     CDS             27130..27789
                     /locus_tag="IV353_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9009438.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            27946..28878
                     /locus_tag="IV353_001173"
     CDS             27946..28878
                     /locus_tag="IV353_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009439.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            29032..30321
                     /locus_tag="IV353_001174"
     CDS             29032..30321
                     /locus_tag="IV353_001174"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9009440.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            30568..31818
                     /gene="clpX"
                     /locus_tag="IV353_001175"
     CDS             30568..31818
                     /gene="clpX"
                     /locus_tag="IV353_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9009441.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            31901..32488
                     /locus_tag="IV353_001176"
     CDS             31901..32488
                     /locus_tag="IV353_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9009442.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            32853..34181
                     /locus_tag="IV353_001177"
     CDS             32853..34181
                     /locus_tag="IV353_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009443.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            34192..34401
                     /locus_tag="IV353_001178"
     CDS             34192..34401
                     /locus_tag="IV353_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009444.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT        11256 a         5953 c         7444 g         9797 t
ORIGIN      
        1 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata agataatcat
       61 catagtttct gaaggtggta tcgactatgt acttgatatc gtcaatgaac cgatggtact
      121 ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggagaac aagcgatgcg
      181 gctggtacag ttaagtgaaa aaacaagaaa ctaaaaatgc ccggtcgtca tgaagcgacc
      241 gggcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc
      301 attttgcgga ttttcaaaga tttgttgagg cgttccgtct tctaaaaagt ttccgtcgtc
      361 aatgaacatg acacgattgg ccacttcttt agcgaagccc atttcgtgcg taacgataac
      421 catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc
      481 ggggtcgagt gcagatgtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc
      541 acgagcaata gccacacgct gcttttgtcc accggaaagc atttcaggat acatatcttt
      601 tttatcttct agaccaactg tctgcagcaa tttgattgct tttttctctg cttcttctct
      661 agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg
      721 gaataggtta aagtgctgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt
      781 gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa
      841 acgattcaag caacgcaaaa atgtactttt tcctgaacct gaagggccaa tgatacagac
      901 gacatctcct tcgttgatgg acacattgat gtcgtttaat actgtgttat cgccgtattt
      961 tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa
     1021 cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt
     1081 aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga
     1141 ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga
     1201 atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat
     1261 aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc
     1321 ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa
     1381 ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa
     1441 gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag
     1501 cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa
     1561 aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt
     1621 tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg
     1681 aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt
     1741 tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg
     1801 catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg
     1861 cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt
     1921 cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt
     1981 cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat
     2041 cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa
     2101 agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa
     2161 tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt
     2221 ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc
     2281 cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa
     2341 ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg
     2401 ccgttgtagt gttgtcagct tgtgcaacat gtccaaacgc aagtgtcaca agtcctgtca
     2461 ttaagaaaac aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc
     2521 aatattttat tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa
     2581 tgaaaaaaat gcgagtttct aacattttat gttataaaac gtaacatgca taataagata
     2641 aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt
     2701 tccttttctt tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat
     2761 gattgagaga gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga
     2821 tcagcctgaa gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat
     2881 cttattaggt gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt
     2941 gaataaacca acactaatta ttgcccataa caaaacattg gctggtcagc tgtacgggga
     3001 attcaaggaa tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta
     3061 tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa
     3121 tgatgagatc gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt
     3181 gatcgtcatt gcatccgttt catgtatttt tggattaggt tcgccttttg agtaccaaaa
     3241 acaagtagtt tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt
     3301 agtcagtatc caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg
     3361 cggtgatgtc gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt
     3421 ttttggagat gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg
     3481 agaaacggaa cacgtggcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat
     3541 ggagcatgcc gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa
     3601 tgaaaacaaa cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat
     3661 gatgttagaa atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg
     3721 taaagaaggt gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt
     3781 ggcggacgaa tcgcacgtaa ccatgcctca agtcagagga atgtataatg gtgaccgagc
     3841 gagaaaacag atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc
     3901 acttcggtta gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc
     3961 aggaccatat gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg
     4021 gctgcttgac ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg
     4081 cgaaataaat gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa
     4141 aatggcagaa gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca
     4201 tagtgatatc aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt
     4261 tgatgtgtta gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct
     4321 tgttgctatc ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca
     4381 aacgattgga cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt
     4441 aacagattct atgcgtctag cgatggatga aacatcaaga cgccgtacga ttcagcagaa
     4501 gtacaatgaa gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat
     4561 ttctattacg aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtatgaaga
     4621 aatgacgaaa gaagaaaaag atacgttatt gatgaaatta gaaaaagaaa tgaaaaatgc
     4681 ggcgaaagca ttggattttg aaacagccgc taatgtgcga gatatgattt tagaattaaa
     4741 agcttcaaag taggaggaca acatggctaa cgataaaatt acgattcacg gtgcacgtgc
     4801 ccataacttg aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg
     4861 tttgtccggt tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg
     4921 gagatacgta gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc
     4981 ggacgtagat agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag
     5041 taaaaatcct cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt
     5101 gtttgcacgt gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc
     5161 agtagatcaa atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt
     5221 ggcaccgatc gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa
     5281 agagggatac gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga
     5341 gctggagaaa aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga
     5401 agggattcgt tctcgtctat ttgattcttt agaggcggct ctgcgattgg cagacggcta
     5461 tgctctggtt gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc
     5521 ttattgcgga tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt
     5581 tggagcttgt ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt
     5641 gattccagat agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag
     5701 ctctcagtat tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga
     5761 tacaccattc gaagaattac cggaagagca tcaggatatc atcttgaatg ggacagatga
     5821 tcttttccat ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt
     5881 cgaaggggta ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg
     5941 tgagcaaatg cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt
     6001 gaatccccaa gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt
     6061 gtctattcaa aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt
     6121 aattgccaaa ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg
     6181 actagactat ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg
     6241 aatcagattg gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga
     6301 accatcgatc ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat
     6361 gcgtgatctt ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc
     6421 agattatctg attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc
     6481 aggcactcct gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg
     6541 gaaaaaatca atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat
     6601 cactggtgct tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt
     6661 tgtagcagtc acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa
     6721 gaaatcgtta gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat
     6781 ttccggctac gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac
     6841 gccgagaagt aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc
     6901 tcaaacgaac gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa
     6961 aggcggtcgt tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt
     7021 gcctgatgtc tatgttcctt gtgaagtatg tcatggcaaa cgatataact ctgaaacatt
     7081 agaagtgcat tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaagatgc
     7141 tgtagaattc ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt
     7201 tggcttaggt tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca
     7261 acggatgaaa cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact
     7321 agatgaacca acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca
     7381 aagattagta gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa
     7441 aacagcagat tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct
     7501 tgcgactgga acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt
     7561 gaaaaaaata atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa
     7621 tgaatttttg cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc
     7681 tacactgatt ggtatagaca aaaacttcct ataagttcaa tccgttcaaa aaagaattga
     7741 gcaatcggag gagtttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag
     7801 ttggaaaaga aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa
     7861 atgaagaatt tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt
     7921 cttttaatga agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta
     7981 cccgctacag taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtgt
     8041 ttatcgagac gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc
     8101 ttttgattgc ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt
     8161 cagaatattt tatctgtcca tatattgatt attctttatt tgaaagactg atcaataaag
     8221 taagctttta tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca
     8281 gagaagaaat gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg
     8341 ctttgaaacc tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag
     8401 cttttatttt agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg
     8461 gttataaaag tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg
     8521 tgctaaatgc gtatgtagat gaagatcatc aagttcggat gatgtgtctt ggtcatccac
     8581 tgttagaaga tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata
     8641 acgaaaaaat ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg
     8701 ccaatttcga tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt
     8761 tgagacaagg tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgtttcg
     8821 tcacagaaga tcgagtgttt aacaaaccct ttgttgaaac gacctatgga acgaatcaat
     8881 cagataaagc aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg
     8941 aaaatgaaga caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg
     9001 tgttttatga aaaagaccgg tcaatcaagc gctggttgtt gatgaaatat atgttccata
     9061 actatattcc aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt
     9121 ttttcagtat tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca
     9181 accaccgaac gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg
     9241 caacagttcc tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt
     9301 ttctgctgga tgatattgag aaacaaaatt tattgcagcc gaacttcatt gtgttgactt
     9361 gtaacaccgc acactacttt tttgaagaat tacaagcggc aacggatatt cctatccttc
     9421 atatgccaag agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga
     9481 ttctaggaac agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg
     9541 gattcgaaac agtgattcct gatactgttc ttcaagaaaa aatcaattat ctgatctatc
     9601 atgagatcaa ggaatcggat tatttgaatc aagagctata ttacgaaatc ttagaagaag
     9661 cggtagagcg cctaaactgt gaaaaagtta tcttaggttg cacagaattg tcattgatgc
     9721 atgaatttgc tgaagataac cattatccag tgatcgatgc ccaatctatt ttagcagatc
     9781 ggacgattga acgagcgcta gctgaaagaa gcaaggcatt ggatacagca tcggaaaagt
     9841 aaaaaaattg gatttggggc aaatatccta aaagatattt ggtccaaatc tttttgtaaa
     9901 agaaaaagaa aactggtaat gatacgcccg gaatgatata attttactat gcacaatgtg
     9961 agaggagttc gtaattatga cagataattt gcaattagtc atcataacag gtatgagcgg
    10021 tgcagggaaa acagtagcta tccaaagttt tgaagatatg ggctattttt gtatcgataa
    10081 tatgccgcct cgattgattc caaaattctg ggaattaatc aaagaatcag gcaaagtcac
    10141 aaaaatggct ttaggagtgg atttacgttc tcgttccttc tttgaagaaa tccaaaacat
    10201 gctgatcgaa atcgaaaata cgaacttcat cgacacttct atcttatttt tagatgcttc
    10261 agacgaagaa ctagtggctc gctacaaaga aacaagacga gcacatccaa tggcgatgga
    10321 cggtttgatc actgaaggga tacgtaaaga acgggcgata ctagacgact tgaaagccca
    10381 agcttccgtg atcattgata cgacgacatt gacaccacga caattacgtg aaaaaatcaa
    10441 tgagtcattc aaatcaaagg gagaccaagg atttcggatc gaatttgtat catttggttt
    10501 taaatacggg ttgccgatcg attcggatat tgtgatggat gtgcgttttt tgcccaaccc
    10561 tcattatatc ccggaactaa gaccattgac cggaaaagat cagccagtct atgattatgt
    10621 gatgtccttt gatgaaacgc aatcattcta ccataaattt ttggatcttc tggaaactgt
    10681 attgcctgga tacattaaag aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg
    10741 cggtcaacac cgttctgtgg cattgactga acgtttggca aatgctttag ggaaaaacta
    10801 taaagtcaat gtgacacatc gggacaagga taaaaggaaa gagacggtga accggtcgtg
    10861 aaaatgaaaa cctaccggat aagaaaacca aagatcgtag tggtcggcgg tggcactggt
    10921 ctgccagtta tcctgaaaag tttgcgaaat caaggagcag atatcacagc agtagtgact
    10981 gttgcagatg atgggggaag cagcggtgca atcagagaat cgatcgctat ggctcctcct
    11041 ggtgacttga gaaatgtctt agtggcgtta tcggatatgc cacaattcta tgaagatatt
    11101 ttccaatacc gtttcaaaaa ggaagatcag tttttagcca atcatacgat cggtaatctg
    11161 atcattgccg cagtttccga gatgcgggga agcacttatg aggcgattca attattagca
    11221 aaaatgatgc atgtagatgg acatgtttat ccttcttcag aaactccttt gaccttgcat
    11281 gctgtcttta aagacggaac gactgcagta ggagaatcta aaatcgcgat cgaccgtaaa
    11341 accattgacc gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa agcggctcga
    11401 aaagtcgttt cagcaattat ggacgccgac atggtcgttt tgggacccgg aagtttattc
    11461 accagtatcc ttccaaattt agtgatacca gaaatcggcg aagcaatgct caagacaact
    11521 gctgaaaccg tctatatctg taatatcatg acccaaaaag gggaaacgga acatttcaca
    11581 gatgccgatc atatacgtgt tttacacaag catttgaatc agccttttat cgatacagta
    11641 ttggtcaata cggaaaaagt gcctgaagat tatatggacc cagagatcta tgatgagtat
    11701 ctagtacaag tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg agtgatttcc
    11761 accgactttt tggaattgaa agatggtggt gtcttccacg atggagacaa agtagtagaa
    11821 gaattgttcc gtttagtttt cggttcaaaa tattaagcaa tttattgttg gagaaaggag
    11881 gagaatagga tgtcatttgc tgcagatgtg aagaaagaac taactggttt ggaagttcat
    11941 cgagaacatg cgaaagcgga attagctgct ttgctgcgaa tgaacggctc tttaggtatt
    12001 gtgaaccagc agtttatttt gaacgtccaa acagaaaatg ctgcaattgc cagacgtatc
    12061 tattctcttt tgaaagatca ttatcaggta cgaagtgagc tactcgttcg taaaaaaatg
    12121 aaattaaaaa agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa agatgtgttg
    12181 atggatcttg atatcatgga tggtatgatg ttcaatggac acgtttcgga cgagattatg
    12241 ggaaattcgc aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc aggttcagtc
    12301 aataatccgg aaacgagccg ttatcatttg gaaatatttt ctatttatga acagcataac
    12361 caagacattt gcgatatgtt aaattattac ggtctacatg ctcgaacgct tgaacggcgc
    12421 aatggctata tttcttattt aaaaggagca gaaaaaattg ctgatttctt aacattgatt
    12481 ggtgcaacca actcaatgct ccgatttgaa gatgttcgaa tcgtacggga tatgagaaat
    12541 tcggtcaatc gtctagtcaa ttgtgagaca gcaaatctga acaagacgat tgatgcggct
    12601 tctaaacaaa tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca tgcccttcct
    12661 gaaaaacttc aggaaatcgc agaacttcgg cttcaacatc cagaggtcag cttgaaagaa
    12721 ttaggggaaa tgattccctc aggtgcgata tcaaaatcgg gaattaatca ccgaattcgc
    12781 aaaataaatg aatttgcgga aaaactgcgt gaagaagcag tttaatccaa agaaaaaact
    12841 gatacatgaa gcaggagatg cgaatgtatc agtttttttt aaaagttata cttgcttttc
    12901 ttccgtcaaa ctataattcc atctttactg gagtaacata atttggaaag gcttctattg
    12961 tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttgtac
    13021 ataaattttt gcttaatata gaagtcctga tatagataag atcaagtatt tatctataac
    13081 tggcctgatt aattaaaaaa ctggaaggaa ctgctcgatc atccttgaga actgctcttc
    13141 ttacttctgg tcaagattct cgtggttggt ggataaggat ggttgataag ttcattggat
    13201 tagctagtcg aaaacaacca ggtgtaatta atgtagaagt tttaacaagg ggatggagtg
    13261 atttattcag tgataatcat aagttcataa atagtaattg agatgtatgg ttggaatacg
    13321 ttatacctga acttgaacta ccaagatttg gtttagaatt tgagaatcga tgtttataaa
    13381 gggagggcaa aaatgcgttt ttttacaact tttactatca ttatgattgc agtacttttt
    13441 atttttctag atatagcaaa gcgaaataca gcatttttat tatatcgggt tttacttcga
    13501 gcaggtctca taacatttat atcgatcgtt ggattttttc tattcacagt tattgtattt
    13561 atttggagaa ctccagcacc accattgcca gagattacat atggagaatt tccttttaga
    13621 cttgagtatg aattgaatga agaattacat gtgatagaag acacgttaat agttgaattt
    13681 gatggatttg gaatgaacga aggcataggg agatatcgta gatggacaag tcgtttagcc
    13741 agcggagaag atttagtgtt gctgttagaa gttagtgata ataagcaaat attttatttt
    13801 ccaggacctg ctaattatta catgggagat aggttgaatg gttataatca tacttttcct
    13861 agtgcttctt tcattgaaag ggaaagaggc attactagaa gggatatttt acatgacaaa
    13921 gaattattag aacaatttgg tccgcttgat caaaatacaa taaatgaaga agaattgtta
    13981 aatcaatata atataaggct agtgaattgg gaaatatctg aaccgattgt taacaatttt
    14041 ggtgattaga aaacaattac gataaataga aacaagccaa atcatcagca agtaaaagct
    14101 gatgatttgg cctttcttat acgagtagta ggaattcata gcgaatatta ttacgatcag
    14161 tagatatttt gatattaaag gagtattacg aactagtatg tgagaatgta gaggtgattt
    14221 gcgaataatg agaaatttat caaatagaaa caaaatatta atcattattg ttgttatagc
    14281 tgtgtttcat ctgggaacga atgcggtttt aagtagaata atattgggtc ctaagccacc
    14341 aagacctgcg attacccgag gggagtttga ttttagatta gaatatgaag tagatgggga
    14401 aaggattgtg atagaagata caattgttgc attatttgat ggattttctg cagatgcagg
    14461 tagcatggca tggtatcgca cgtggcgttt gcatttggcg agtgacaaga gggcacaaca
    14521 attattgtta catgagttag atgatggtcg aaaaatatat tatcattttg gaggagctgg
    14581 attttatatg gggaagttag tagcagtgta actgtaacaa ggaaagattt gccaggtctt
    14641 ggcattcgaa actccatatt ttatatcgag cgtgggagag atacatctat tgggaacagg
    14701 aaagggttaa ctcttgaaga gttatataat gactttggaa tccgtcttgt tagttgggaa
    14761 cacaatccac caatagaaaa tcgctttgaa taatcgaaat ctattaatcc atttcatcag
    14821 ccagtgaatg ttgtaatgga acaatttggt ttagtgttca aaaccattga aatactaggc
    14881 tgtgatcagc aaaaatgtat ctgaataaaa aagagagcaa ctattaaatg agaggagtta
    14941 ggagttgtta gctttatacg aatcaaagtg gcaaatacag atgcgtatgc atttgtgttt
    15001 gccatcttgc gtttggtaga cgagataggt ataagctaaa gaaacaactt gtttaaaata
    15061 aacgacctag ctcaacaaac gaccatctaa tcaactaaca gcatacataa aagcaactat
    15121 ttttgaagta gaattgaaat cgggataact taaaaagtga ttgggatact agtgtgaatc
    15181 accgtacata aatattactc gttaagggaa tttgagaaat tttgtatgaa aatttaagaa
    15241 ttagttagta tgcgcaagtc aaaaatatga ataaattatt tggtggagat aatttgctca
    15301 gcttgtcata agaaatgaga taatctggca gatgcaagct tttggacacc atttattcgg
    15361 aataaggagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt tgcttctgct
    15421 tccgccgttt ataggtgttt agagcgtgag acaaaagtgt tctttacttt tgtcctacgc
    15481 tccttatttt tttattttgt ttggatcaat tcttttaatt cctctattga aatagattca
    15541 tcaaagattt ctttacccaa gatgacagtc ggaacaaatc gaatatgtgc ttgctcagct
    15601 tcgttgatga cagcttggct cagctgttcg tctttttgtt ctgtcagctt tagtttatct
    15661 actgcaaaat ccgctacttc ttggagagaa agatgtttcc actcgtcttg agtatcgaat
    15721 atttgtttga tctctttgat cgctttttga ccatcgctga ttgtgagata ccggtgcatc
    15781 acgtttcctc gctgcaagct ttctttttct ttatcgaaca atttgatcac tcgctgcaat
    15841 tgcccgcttt ggactgcttc ttctaatagc tcataagatt cttcaaacca ttgtcggcaa
    15901 tacggacaag ataggttgat gaattcgacc atttttttag gagcattcga tgaaccataa
    15961 aggattcctt tttgtgtatt tgttttcgtt gcatcaatca ctgatatatc catgttaaaa
    16021 cacctctttc gcttatagtt tatacatgtc tgacaaaaaa aggcaacgat ttagactgtt
    16081 tgtctaaaaa ttagatgacc aattcaaata gacatatgtg aaatattttt ataaataaaa
    16141 gaaaaaatgg aatgttttac gttataatgg aacagataat aagagagaat atgaacaagg
    16201 atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat acggcagccg catatatttc
    16261 ttatgcattt accgagattg ctgcaatata tccaataaca cccagttcga ccatggctga
    16321 attagtagat caatggtcag cagcaggaaa agaaaatgta ttcggtcaac cagtaaaagt
    16381 agtagaaatg caatccgaag caggagcagc tggtgtggtc catggatcat tgaaaactgg
    16441 tgcactgaca actacatata ccgcttctca agggctgctt ttgatgatac cgaatatgta
    16501 caagattgct ggggaactac tgccaagtgt tttccatgtt gctagtcggg cagtgacgac
    16561 aaatgcgttg aatattttcg gagatcacgg agacgtgatg gctgcaagac aaacaggatt
    16621 tgctatgtta gctgaaagca gtgtacaaga agtgatggat ttagcacctg ttgcccatct
    16681 tgcttctatt gaaggagaag tacctttttt gaatttcttt gatggattcc gtacgagtca
    16741 cgagattcaa aaaatcgaag ttttggaata tgaagaatta gctcctctga tcaatcagga
    16801 gaaattagct tcatttcgta aacgagggat gaatcccaat catccgactg ttagtggtac
    16861 gaaccagaat ccagacatcc attttcaaca acgggaaacg atcaacccct attatgataa
    16921 attacctgaa atcgttcaaa aatatatgaa agagatcaac cgattacgag gaacttctta
    16981 tgacttggtg acttattacg gagcagacga tgcggaagaa gtcattgttt ctatgggatc
    17041 agttgcacag acgattgaac aaacgattga ttacttgaat gcaaacggac ggaaaacagg
    17101 ttttttgaat atccatcttt atcgtccttt tccagtggaa acattcgtgc aaaagttgcc
    17161 tcatacagta aaaacaattg ctgttcttga tcgaagtaaa gaaccagggg caggtggaga
    17221 acctctctta ttagacgttc aaagtgcgtt atatgattct gaacttcgcc cggctgttat
    17281 tggtggtcgc tacggattgg ggtctaaaga tgtgactccc gaccaaatct cagcagtttt
    17341 tgatgaattg aaaaaagacc caagcatacg caaaaaacgg tttaccatag ggatcgtgga
    17401 tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg gatttgacag aaccgcaaac
    17461 attccaggcc aagttttggg gatttggatc tgacggaaca gttggagcaa ataaatcagc
    17521 tatcaagatt attggagatc acaccgataa atatgcgcaa ggctattttt attatgattc
    17581 gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt ggagatacgc cgatacgttc
    17641 agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac acaccagctt acctgcatag
    17701 ctatgacttg gtcaaaggat tgaagccagg tggcattttt ttattgaata ctctttggag
    17761 cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa cgttacttgg ctgaaaataa
    17821 cattagattc tatacgatca atgccatgag attggcccaa gaagtcggtt tgggacgccg
    17881 gatcaatacg gcaatggaaa cggctttttt taaattagct gacatcattc cttttgatga
    17941 ggtgcttccg ctgctaaaag aagaagccct gaatagctat ggacataaat caatggctat
    18001 cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa ctgcttcatc aagtcgcgat
    18061 cccagaccat tggagaacgt tggagatgcc tgaaaaaatc aataaaagga acactggtta
    18121 tatacaagaa atacttgaac cagtgaatgc acaggaagga aatcaactct ctgttggtac
    18181 acttgtagca aatggaatga caactggcga aatgccttta ggaacggctt ctatggaaaa
    18241 gcggggcgtc gctttagaag tacctgagtg gattagtgat cgctgtacga tgtgtaatga
    18301 atgtgccttt gtttgtcctc atgctgccat ccgtccattt ttagcagatg aagaggaaat
    18361 ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga ggcgcagacg gcgtaaaata
    18421 ccgtatccaa gtttcagtgg aagactgtac aggttgtggt ctgtgtgtag aggcttgtcc
    18481 cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa cagaaagaac aagctgtgaa
    18541 ttgggctttt gcaatgacat taagacaaaa agcaaatcca gtcaaaggaa agaatactgt
    18601 cttgtctaca caattcaatc aaccgcttct tgaattctcg ggcgcttgct caggctgtgg
    18661 cgaaacacct tatgtaaaat tattgacaca aatgttcggt gatcgaatga tgatcgcgaa
    18721 tgcgacaggc tgttcttcta tttggggcgg tgcttctcct gcttctcctt atacgaccaa
    18781 tgaatgtggt caagggcctg catggtcaaa ttctttattg gaagataatg ctgaatttgg
    18841 ttatggtatg cttctagcgg cacagactcg tcgcgaggct ttggcaagtt caatcaaaga
    18901 agtattgcca aaagtatcag aagaactacg agaattgatg gaagactggt tggtacacat
    18961 gtttgagagc gaaggaacgc agcaaagagc agcaaaactg caagctgcga tcgaaagcga
    19021 acgatcaatt gtccctgaac tggaggatat atacaagaga aaggatctat tcgtaaaaaa
    19081 cagccaatgg atgattggtg gagacggatg ggcttacgac attggttttg gcgggatcga
    19141 tcatgtactt gccagcggag cagacgtgaa tattcttgtt ttggataacg aagtctattc
    19201 caatactggc ggtcaaacct caaaagcaac accagcttcg gcaatcgcta aattttcggc
    19261 tagtggaaag tatgcttcaa agaaagatct aggaatgatg gcgatgacat atggaaatgt
    19321 ttatgtggcg caaattgctt caggtgccaa tcaaatgcag acgataaagg cttttgaaga
    19381 agcagaaaag tatcctggac cttctttaat cattgcctat acgccttgta tcacacatgg
    19441 tttgcttggt ggaatgaaag aatcgcttaa agaagcaaaa gaagctgttt cttctggcta
    19501 ttggtcatta tatcgatata accctgcaat ggtcgaaaaa aacaagaacc caatgacttt
    19561 ggactacaaa aaacctgatt tctctgctat gcctgatttc atgaggaaac aagttcgctt
    19621 ttcctcgcta gagaacgcaa atccaaatct tgcaaaagct ttatttgaaa agactatcca
    19681 agacgctaaa acacgttttt acaactatgc tgcactgacg ggagaattag aaaaaatcaa
    19741 gaaacgactt gaaccagagg aatctatcga tgtacccgtt aagactagaa aagaaaaaac
    19801 gacggacctt gaagcagaag aaagaagagc tgccagagca gaacgacgta aaaaaatgga
    19861 acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat aattctctga
    19921 agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt tcctgaaaat
    19981 tatgcgtttt tctcaagaaa aatacgagca gatcagcctc taaaattaaa acatatgata
    20041 tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca aatcggggaa
    20101 ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc tctgaatttt
    20161 ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact gatccaactg
    20221 ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat tgttatccga
    20281 atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca agtcattacg
    20341 tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg actggaacat
    20401 cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga tgagaagatg
    20461 atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg tgcgctaatt
    20521 actatcgaac ggcatactgg attagatgag tatattgaga caggaattgc attagatgca
    20581 gatatcactg gagagctgct gatcaatatc tttattccta atacaccgct gcatgacggt
    20641 gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc tctatcagaa
    20701 agtatgttga ttccaaaaga attcgggact cgacaccggg cagctgtcgg tataagcgaa
    20761 gtcagcgacg cgattaccat cgttgtctct gaagaaactg gggatgtcag cattacatta
    20821 gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatttt gcgtagagaa
    20881 ctgattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt cctagacggt
    20941 atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc attctttaca
    21001 gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc cagtccgttc
    21061 caaatacgct ttctggaaat gaagcgtata atcaaacagt tactggggtg ccaatcaaga
    21121 tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg actgtcaaac
    21181 tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca cggatgttcc
    21241 gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaggttccg ctaaaaattc
    21301 aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca gtaacggttg
    21361 agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg ttcacaccat
    21421 ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc actacaggtg
    21481 acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg atggtcacag
    21541 atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg gaagtattaa
    21601 gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag ccaagcaagt
    21661 cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa agctatgatt
    21721 tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt gcatcgatcg
    21781 gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca actcgtaaaa
    21841 ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt gtggagatta
    21901 cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg acacaagaaa
    21961 atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca tcgacaacaa
    22021 ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca gaagaaacat
    22081 cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat taagttaaag
    22141 gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg gtgaagccaa
    22201 caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt atgtcctaag
    22261 ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata caagaatctc
    22321 aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta tcgaagtgtt
    22381 ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc aaaaagcaag
    22441 cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa tcaaattttt
    22501 cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag cattattaga
    22561 tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg tagaagaatt
    22621 tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg gtgacctttc
    22681 cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg ttaatcgttt
    22741 gtttgccgat cttgaaacag atttttatac catgggcaca aatccagacg ggttaaatat
    22801 caatgatggc gtaggctcta cccatccaga acgtttagct gagatggtcg ttgaaaaagg
    22861 agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg tggatgaact
    22921 tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact tggcagaaaa
    22981 aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag gattccacaa
    23041 agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc gttacgttgt
    23101 agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc acatgatttt
    23161 cttagatttt aacacaactg gagatgggat gctttcagga attcagttac tgaatatcat
    23221 gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct atccgcaaaa
    23281 attagtgaat atccgagtaa gtgataaaaa cggtgcgatg gatgttccgg caatcaaaaa
    23341 agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag ttcgtccttc
    23401 cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga aagttaatta
    23461 ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt aagagatatt
    23521 ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt ccttgttctt
    23581 ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt cgaatccccg
    23641 taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta gagtagttag
    23701 ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc cctctgagac
    23761 aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc gagtatgcgc
    23821 accagtcact tcttcactga tgattccaat cactggtgtt ccttcttcga tcaaagcaat
    23881 cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac tgatttcttt
    23941 caatttcaaa gcagcttcaa gagaaacgtt gtagtcagcg ccacgtccaa tgtaaaaggc
    24001 atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt tttgggcgat
    24061 gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga atacaagaga
    24121 agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta cagctatttg
    24181 tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca atagcagtgt
    24241 aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa gtgaagggta
    24301 gccgagacgg ttgattttta ccagaacttg gcggctatct gctgtttcgc cactttgact
    24361 caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa attcactggc
    24421 taaatgaacc tcaacaggga tctgtgtcaa tttttccaga gtctgtttac ctgctaaccc
    24481 cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag acaatgtatc
    24541 gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt gagcgatacg
    24601 acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat aggtaccttt
    24661 ctcgatatct gaagaatcta cttgtgcttc gaatgaagaa cgttctacga tgtcacctgc
    24721 aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat cttcaatctc
    24781 aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca cattgaatcc
    24841 atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa catcaggtgc
    24901 tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag cttttaaaaa
    24961 cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga taatctcagt
    25021 atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt cttcaaagtt
    25081 ttcgattacg ccattgtgaa cgagcacgaa gcgtccactt tgggaagtat gtggatgggc
    25141 gttttcttca gaaggttgcc cgtgagttgc ccagcgtgta tgaccaatac ctatatttcc
    25201 tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg ctttgaccag
    25261 ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac ggtattctaa
    25321 tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca ttccaacaat
    25381 tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct tccagctgca
    25441 ggaaaatcct tttgttttat ctttgcagat attttttgtg gattttctgt agagtgcagt
    25501 ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg tttacacaga
    25561 cctggcgcta tcctaaaagg tataactatc ctcctctttt gttttttatc tggaaacatt
    25621 taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa aataaattgg
    25681 tatatatcaa atgctttgtc aaatgctctt tttatgtcaa aaaataatgg gtttttatct
    25741 atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag gagtaaaaat
    25801 ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt ccaaattcga
    25861 agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct gggatagcat
    25921 ccaagaaatc gcacctgatg ccttggaagt tgctgaagct cgtgagttta cggtagaaga
    25981 tgaattgaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag agcggttcga
    26041 cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga aaggagaaag
    26101 aatgaataag agacgttggg cagcagttct gattgctgca ggattatttg tggtttcgtt
    26161 agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat tgaacagttg
    26221 gttatatggg gatgaagaac tagcttcaaa tgtgttggaa gagggagaca gtacgcaaaa
    26281 aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag gtttatttgc
    26341 ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg aagaagatcc
    26401 aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct acgaaagtgc
    26461 ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa tgtatgtcag
    26521 catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag ataaaatctt
    26581 tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat tgaattattc
    26641 aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag cgttgaaaga
    26701 tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc aagcatatat
    26761 cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata aatctgaaga
    26821 agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa aagaagtagg
    26881 tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatgcgaa aggaacaaca
    26941 gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg ctagtacatg
    27001 gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta gtcagatctt
    27061 atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt attacggagg
    27121 tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga taagcaacag
    27181 cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc ttttgaaaga
    27241 gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa gtatacggac
    27301 acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc aggttttttt
    27361 atccgaatgt ttgctttctt ggtcgatctt ttgtgtatcg gagctattac gcgtttaaca
    27421 cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct gagtatctac
    27481 ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa attcaatcat
    27541 ggtcaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga tgaagaagaa
    27601 cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct aaaatttcct
    27661 ttgctgatgt tagggtatct tccagcaatc tttaatcaga aaaagcaaca tctaggcgat
    27721 tttttcacta atacaagtgt tgttacgatc aatctgatca aagcattcaa ccaggaagtc
    27781 aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa agtcagatct
    27841 aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt ttttgttttg
    27901 ctactattga acatgagctg aacatgagca agaggaggac actgcgtgaa gccgattgat
    27961 tttccagaaa aatatgagaa tttaatgcga gtagcacaac aagcactagc aaaccagcaa
    28021 tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag ctttgaggct
    28081 aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc attgaagcaa
    28141 gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt ttattttgac
    28201 ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag tacagatttt
    28261 tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga attatccgga
    28321 caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact tccttcgttg
    28381 gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta tcatgagttt
    28441 atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc aagggcaaaa
    28501 ttactggaga cacttaggca attgaacgaa tgccatcctg ttttctattt gacgattgaa
    28561 gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca ggcaagttac
    28621 cagcaattat gtgtattttc agatcgttac ggtaatgaag atgcgctgct tagttccgtg
    28681 ttaaatgaag aattcacgtt gcaatctgca attgtttatc ctgtctatga tacatacatc
    28741 gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa aacgatgtat
    28801 gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat tttgcagcaa
    28861 atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg aaaaacaagg
    28921 attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt aaaggtatgc
    28981 atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta aatgactgca
    29041 acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc gcaagacgaa
    29101 atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa cgtacctgga
    29161 ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga agaagcttta
    29221 tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt caaagaagct
    29281 ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa aggtcaagac
    29341 tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca atataaagat
    29401 ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc acgtatcaaa
    29461 cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc tgaaaatggc
    29521 gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga tggcggaaaa
    29581 ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt tgaagatcaa
    29641 ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc agaagattat
    29701 caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca tgaagtaaaa
    29761 gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga cgaagtatca
    29821 acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa agaaaaagca
    29881 gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc agaaatcgtt
    29941 gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga attcttgaac
    30001 aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg ttctacagaa
    30061 gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac aaatcttgtg
    30121 atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat caatgcagaa
    30181 atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg cgtgttgaca
    30241 gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat cacaggtaca
    30301 gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa gctacttcta
    30361 gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt ccttattttt
    30421 tgttaagagt aaaaaatgca aaggtatagg tcagttgatc ctttgttaaa cactttttct
    30481 ttttactaaa ctatgttatg atagtaaaag actgtaaagt ctttgatagt ttcttgcata
    30541 ctttttgcaa gttaaggggt gaagaccatg tatgacaatc caagtagcaa tgagaccgta
    30601 cgctgttctt tttgcgggaa aacccaagaa gaagtgaaaa agatcgtagc tggtccaggg
    30661 gtctacatct gtaatgaatg tatcgaccta tgtaaagaaa tcattgatga agaattttat
    30721 gaagaagctg tacgcgaatt cactgaagtc cctaaaccac tagaaattct tgaagtattg
    30781 aataactacg tgattggaca agatcgcgca aaacgctcgt tagctgttgc cgtatacaat
    30841 cactataaac gtgtcaatca gcaagcacaa gaaaacacag atgatgttga actgcaaaaa
    30901 agcaatatct gcttgatcgg acctacaggg tctggtaaaa catttttagc acaaacatta
    30961 gcaaaaacat tgaatgtacc ttttgcaatc gcagatgcga caagtttgac agaagctggt
    31021 tatgtggggg aagatgtaga aaacattttg cttaaattgc ttcaatctgc tgattacaat
    31081 gtggaacgtg cagaaaaagg gatcatctat attgatgaga tcgataaaat tgcgcgtaaa
    31141 agcgaaaatg tatctattac tcgtgacgtt tccggcgaag gtgtccaaca agccttgctg
    31201 aaaattctag aaggaactgt tgcaagcgtg ccacctcagg gaggacgtaa acatccacat
    31261 caagaattta ttcagattga tacgacgaac atattgttca tcgtaggtgg tgcatttgat
    31321 ggcatcgaaa cgattgtgaa gaatcgtatg ggcgaaaaga cgattggatt tggtacacaa
    31381 aaccaaaaac tttctgaaga cgaaagtgtc atgcagcata tcattcctga agatttacta
    31441 aaattcggat tgatccctga gtttatcgga cgattacctg ttatggcagc tttagagaaa
    31501 ttgacaacag atgatctggt acggatcttg actgagccga aaaatgcgct agtcaaacaa
    31561 tatcaaaaac ttctttctct agataatacc gaacttgaat tcgaacctga agcgttgaga
    31621 gccattgcga aaaaagcaat cgaacgcaat accggtgctc gaggacttcg ttccatcatt
    31681 gaagaaatca tgatggatgt gatgtttgat atcccttcaa atgaaaacat cgaaaaagtg
    31741 attatcacaa aagaagcagc tgaattgtct ggtgagccaa cagtaatcta taaagattca
    31801 gagaaaaaag cgggttgatg aaaagaggct gccgtaaaag tcattttggc ttttgcgaca
    31861 gccgcttgct ttattcatca ccttgttaag gagagagacc atgaaagtcc atcaagctga
    31921 aattatcata agtgcagtgg caccaaaaca atatccagat acacaattgc cagaaatagc
    31981 tctggcagga cgatcaaatg tagggaaatc ttcttttatc aacacattga ttgatcgaaa
    32041 gaatttagcc cgtacttccg gtaagccagg aaagacccag acattgaatt tttatttgat
    32101 cgagaacgca ttacattttg tcgatgttcc gggatatggg tatgcaaaag tatccaaaac
    32161 tgagcgagca aaatggggaa agatgatcga gacatattta acgactagag accagctacg
    32221 tgcggtcgtt tctttagtag atttgcgtca taagccttct caagaagata tccagatgta
    32281 tcaatttttg aagtattatg atatccctgt aattgttgtt gccactaagg cagataagat
    32341 ccctcgtggt aaatggaaca agcatgagag cgaaatcaaa aaagcgttgg attttgatcg
    32401 caatgacacg tttattctct tttcttccgt tacaaaacag ggaaaagaag aagcgtgggc
    32461 agcaatcgaa caagcaattg aatcataaaa agaataaaaa agacttacca gtcaaactct
    32521 ttcataaaag tttggctggt aagtcttttt ttgagcttaa cttacttttg tttcttgtct
    32581 tttttctttt cattttgtgg ctgtggtttt agaaatcgat cgatctcttc ttttggtgca
    32641 actgtttttt ccggatcgac agcaaatgat tcgaaaagtt tatctaaatt atctgtacgt
    32701 tcaaatttca accccattgt aaacacctct ttgtttcttt ttacttagtc tatcatgtaa
    32761 tcgtccaaat tggtttctaa gacataaagt tttagttttt ctttatcgtt ggttataatt
    32821 aaattataaa aagattttaa agaggtgaga aaatgattgg cgaagataaa ttattgatga
    32881 ctatccagct cacagactta aatgaagaag aaaaaaaaca ggaaataagc gattgggcta
    32941 ttttgacacg tctgttgatc cagccaacat ttatccgctc tttcacacaa caagcggatc
    33001 cgtatgatct tcgaaagctt caagaaaaaa taatgctagc atcggtaaga gacgaaagtt
    33061 ggttagttgt aggaaatgac gagaacgaat gtagttttcg cttggaagat aatcaattat
    33121 tgataaaaaa tgtattgtct atctctgttt tcaaagaaaa gcaatttttg attcgagact
    33181 tcattcagaa aaaaatgatt gctcatgggg tgttcgccta tatgcgagct tatagcgagt
    33241 ttatctatca caatacaaaa cagatctctc aacggctatt gtttgagaaa aaggatgaaa
    33301 tcgaacattt acctaaaatg aaacaacaaa acggggaagt agtagtcgat tgcaaccaat
    33361 ttccaggata cgatcttttt taccaaggtt tatgtttcac ttcgtgttgg gaaatgtatt
    33421 atagtcgcta ttatcatcaa atcatcccta aaccgatttt tctcgacatc caacaagtag
    33481 aaaaagtgaa ggaattagaa aacgaagtca tatgtatcca gctttatcga gaccccttta
    33541 actggcaaaa gccaaacaat caaatgtttc aatcctattt tagggaccag cttgggtttg
    33601 atcatcttgc ttgggacaat ggtgtcggtt tattgaaacc gccattcgtt gaatacattt
    33661 acactgacca tacgatccaa tctgtccagt atcagaatgc acaaatgcaa cctgttccta
    33721 aaaagaatgc ttcatttttt gtaacaaaaa gttatgatat tcaacaagga gattataaag
    33781 aaaggcgtgt tcgcggaaca ctaaatgctc aggcgtattt tccttgggtt gacgagaatc
    33841 gctcgcgcat gatgtgctat aaagttatcg atccgactgt tgctttggat aatggaatcg
    33901 aagcttattg ttattatatt agggaatatc tagaagtaga ggtaacagat gaaaaatatc
    33961 aagagtatct tttatcctta agaatctatg ttccttcaga atccctgtca gaattaccgt
    34021 taaaggaaat caaacataga ctctctgatg ttaccttcaa acgaataaag aaaaagcggc
    34081 gaagtatcca attcgatgtc aagaaaggtg agcagcatct gcgtgtgcaa tttttagatt
    34141 accgagaatt agaacaattg attactttac aaaaaatatg aggtgaagaa aatggataaa
    34201 caactctatt atcgaataat ggttccggca tcattattat ccgtctgtct cttttttgtt
    34261 cctaattttc ctattcgatc agtcatcggt tttttggtca tgtttggcgg gatgggactt
    34321 tattattatc ttcttcataa ggaaagaaaa cctgcttctg tttatatgaa aaaaacaaga
    34381 agcgaaaata aaaaacgata aaacggcttg atgatagaca aaaaaataaa aaaccagtct
    34441 ttttgactgg
//
LOCUS       DACDLI010000024        34243 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDLI010000024 DACDLI010000000
VERSION     DACDLI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.024"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            144..542
                     /locus_tag="IV353_001179"
     CDS             144..542
                     /locus_tag="IV353_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9009445.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="IV353_001180"
     CDS             526..1221
                     /locus_tag="IV353_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9009446.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1815..2702)
                     /locus_tag="IV353_001181"
     CDS             complement(1815..2702)
                     /locus_tag="IV353_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9009447.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2887..3726
                     /locus_tag="IV353_001182"
     CDS             2887..3726
                     /locus_tag="IV353_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9009448.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3922..4896
                     /gene="sgrA"
                     /locus_tag="IV353_001183"
     CDS             3922..4896
                     /gene="sgrA"
                     /locus_tag="IV353_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9009449.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5194..5550
                     /locus_tag="IV353_001184"
     CDS             5194..5550
                     /locus_tag="IV353_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009450.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5553..6710
                     /locus_tag="IV353_001185"
     CDS             5553..6710
                     /locus_tag="IV353_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009451.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6736..7176)
                     /locus_tag="IV353_001186"
     CDS             complement(6736..7176)
                     /locus_tag="IV353_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9009452.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7336..7842
                     /locus_tag="IV353_001187"
     CDS             7336..7842
                     /locus_tag="IV353_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9009453.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8010..9923
                     /locus_tag="IV353_001188"
     CDS             8010..9923
                     /locus_tag="IV353_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9009454.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9928..10929
                     /locus_tag="IV353_001189"
     CDS             9928..10929
                     /locus_tag="IV353_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9009455.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11131..11337)
                     /locus_tag="IV353_001190"
     CDS             complement(11131..11337)
                     /locus_tag="IV353_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9009456.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11983..12831)
                     /locus_tag="IV353_001191"
     CDS             complement(11983..12831)
                     /locus_tag="IV353_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9009457.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12995..13897
                     /gene="gnd"
                     /locus_tag="IV353_001192"
     CDS             12995..13897
                     /gene="gnd"
                     /locus_tag="IV353_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9009458.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13899..14900
                     /locus_tag="IV353_001193"
     CDS             13899..14900
                     /locus_tag="IV353_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9009459.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14897..15181
                     /locus_tag="IV353_001194"
     CDS             14897..15181
                     /locus_tag="IV353_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9009460.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15214..16011
                     /locus_tag="IV353_001195"
     CDS             15214..16011
                     /locus_tag="IV353_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9009461.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16025..16858
                     /locus_tag="IV353_001196"
     CDS             16025..16858
                     /locus_tag="IV353_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9009462.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16876..17733
                     /locus_tag="IV353_001197"
     CDS             16876..17733
                     /locus_tag="IV353_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9009463.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17755..18264
                     /locus_tag="IV353_001198"
     CDS             17755..18264
                     /locus_tag="IV353_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9009464.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18301..18702
                     /locus_tag="IV353_001199"
     CDS             18301..18702
                     /locus_tag="IV353_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009465.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18814..19167
                     /locus_tag="IV353_001200"
     CDS             18814..19167
                     /locus_tag="IV353_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009466.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19201..19644
                     /locus_tag="IV353_001201"
     CDS             19201..19644
                     /locus_tag="IV353_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9009467.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19661..20746
                     /gene="uxuA"
                     /locus_tag="IV353_001202"
     CDS             19661..20746
                     /gene="uxuA"
                     /locus_tag="IV353_001202"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9009468.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20739..21452
                     /locus_tag="IV353_001203"
     CDS             20739..21452
                     /locus_tag="IV353_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9009469.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22204..22404
                     /locus_tag="IV353_001204"
     CDS             22204..22404
                     /locus_tag="IV353_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9009470.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            23100..23534
                     /locus_tag="IV353_001205"
     CDS             23100..23534
                     /locus_tag="IV353_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009471.1"
                     /translation="MIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGND
                     HSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAV
                     LLNHVRKCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(23595..23960)
                     /locus_tag="IV353_001206"
     CDS             complement(23595..23960)
                     /locus_tag="IV353_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009472.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(24036..24281)
                     /locus_tag="IV353_001207"
     CDS             complement(24036..24281)
                     /locus_tag="IV353_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9009473.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(24351..24605)
                     /locus_tag="IV353_001208"
     CDS             complement(24351..24605)
                     /locus_tag="IV353_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009474.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(24769..25092)
                     /locus_tag="IV353_001209"
     CDS             complement(24769..25092)
                     /locus_tag="IV353_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009475.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            25279..25352
                     /locus_tag="IV353_001210"
     tRNA            25279..25352
                     /locus_tag="IV353_001210"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25313..25315,aa:Ile,seq:cat)
     gene            complement(25737..26234)
                     /locus_tag="IV353_001211"
     CDS             complement(25737..26234)
                     /locus_tag="IV353_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9009476.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(26274..27083)
                     /locus_tag="IV353_001212"
     CDS             complement(26274..27083)
                     /locus_tag="IV353_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9009477.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            27417..28274
                     /locus_tag="IV353_001213"
     CDS             27417..28274
                     /locus_tag="IV353_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9009478.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            28267..29169
                     /locus_tag="IV353_001214"
     CDS             28267..29169
                     /locus_tag="IV353_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9009479.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            29202..29552
                     /locus_tag="IV353_001215"
     CDS             29202..29552
                     /locus_tag="IV353_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9009480.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            29586..30524
                     /locus_tag="IV353_001216"
     CDS             29586..30524
                     /locus_tag="IV353_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9009481.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            30602..31495
                     /locus_tag="IV353_001217"
     CDS             30602..31495
                     /locus_tag="IV353_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9009482.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            31532..32095
                     /gene="amaP"
                     /locus_tag="IV353_001218"
     CDS             31532..32095
                     /gene="amaP"
                     /locus_tag="IV353_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9009483.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            32109..32297
                     /locus_tag="IV353_001219"
     CDS             32109..32297
                     /locus_tag="IV353_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9009484.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            32310..33212
                     /locus_tag="IV353_001220"
     CDS             32310..33212
                     /locus_tag="IV353_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9009485.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKNGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            33244..33486
                     /locus_tag="IV353_001221"
     CDS             33244..33486
                     /locus_tag="IV353_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9009486.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(33742..34113)
                     /locus_tag="IV353_001222"
     CDS             complement(33742..34113)
                     /locus_tag="IV353_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009487.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT        11922 a         5435 c         6874 g        10012 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
     1861 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
     1921 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
     1981 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
     2041 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
     2101 atctctggat tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt
     2161 tttccataag cttgaacaag cttggagaaa ttttctcctt catttgcttc tttttttgct
     2221 ttatctgctt cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat
     2281 tctttccaag cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat
     2341 tggaaagcca gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct
     2401 tttaattgtt ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt
     2461 acttcctttg aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt
     2521 ttttttaata gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact
     2581 gtaagtgttc cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat
     2641 agtaaaattg cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc
     2701 atcctttcct tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg
     2761 taaggttgct gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat
     2821 ttacatagtt ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga
     2881 tgaaagatga tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt
     2941 gttttaggaa ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg
     3001 attggagcaa ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt
     3061 gctagtaagg tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac
     3121 tgggggtatt tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt
     3181 ccattatatt tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg
     3241 ataacgagtt ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc
     3301 ttttttcagc atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca
     3361 ttatttgctc ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc
     3421 tttttagcag gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct
     3481 attggtgctc atattacttg gaattggaca caaggagcta ttttgggtat accagtcagc
     3541 ggaacgcaag aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact
     3601 gggggaaact ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt
     3661 gttggtttgt tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat
     3721 caataaaaga tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga
     3781 ttaaacgaaa aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca
     3841 actgactgtc gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta
     3901 attatatgga gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg
     3961 ctgagtataa ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag
     4021 aatctttcat ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt
     4081 actattccag tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca
     4141 tatggttggg atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac
     4201 gtacaatgga ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat
     4261 gagatcaaga gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc
     4321 acggagaatt tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc
     4381 acaagtagtg aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc
     4441 agtgaaagta gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa
     4501 agtagcacag gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc
     4561 acaggaagca tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga
     4621 aaagtaaata atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat
     4681 gcagggagca acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat
     4741 catcaagaaa aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt
     4801 agaaccttga acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca
     4861 agctattttc gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa
     4921 ttgaataaaa cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc
     4981 gaagttgtag ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata
     5041 acaggaacac aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta
     5101 gtatctctga aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata
     5161 agtctaaata atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat
     5221 gaaaggcaag tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg
     5281 gtgaaggata tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa
     5341 caagcagaat cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac
     5401 aagatgataa aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt
     5461 ttagagggaa cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc
     5521 ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag
     5581 aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga
     5641 tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg
     5701 acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat
     5761 ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa
     5821 gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga
     5881 acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt
     5941 tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt
     6001 attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata
     6061 aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact
     6121 tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc
     6181 atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag
     6241 aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta
     6301 gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga
     6361 tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat
     6421 tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat
     6481 ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca
     6541 ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact
     6601 ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca
     6661 ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga
     6721 ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg
     6781 accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct
     6841 gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt
     6901 tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc
     6961 tattctgtga acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc
     7021 gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa
     7081 gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga
     7141 cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt
     7201 tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa
     7261 aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag
     7321 aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa
     7381 ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat
     7441 aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat
     7501 gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag
     7561 cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt
     7621 ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt
     7681 atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga
     7741 gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag
     7801 aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa
     7861 aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat
     7921 cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac
     7981 atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa
     8041 agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg
     8101 gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg
     8161 ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg
     8221 agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag
     8281 aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca
     8341 aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat
     8401 atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca
     8461 agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata
     8521 aagagattat caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa
     8581 acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca
     8641 caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca
     8701 aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc
     8761 atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt
     8821 ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg
     8881 attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag
     8941 ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga
     9001 tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg
     9061 gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac
     9121 taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat
     9181 ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg
     9241 ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag
     9301 atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag
     9361 atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct
     9421 acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt
     9481 atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag
     9541 cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg
     9601 ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca
     9661 aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg
     9721 gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag
     9781 tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga
     9841 aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga
     9901 tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta
     9961 ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag
    10021 ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc
    10081 cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat
    10141 ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa
    10201 ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt
    10261 cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat
    10321 gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa
    10381 gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat
    10441 tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa
    10501 aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg
    10561 tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt
    10621 tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc
    10681 aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat
    10741 ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg
    10801 ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat
    10861 cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc
    10921 aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca
    10981 aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag
    11041 gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg
    11101 tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa
    11161 agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa
    11221 gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat
    11281 agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag
    11341 cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag
    11401 tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat
    11461 aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca
    11521 ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc
    11581 caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa
    11641 taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt
    11701 aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag
    11761 aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg
    11821 aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta
    11881 ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg
    11941 ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga
    12001 aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa
    12061 aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga
    12121 tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga
    12181 agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc
    12241 aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc
    12301 cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat
    12361 aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc
    12421 gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg
    12481 ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc
    12541 catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc
    12601 attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt
    12661 tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg
    12721 actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg
    12781 gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc
    12841 tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag
    12901 agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt
    12961 gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg
    13021 aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact
    13081 cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga
    13141 gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc
    13201 tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat
    13261 tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca
    13321 gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca
    13381 tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt
    13441 caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt
    13501 cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt
    13561 ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa
    13621 tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca
    13681 tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt
    13741 tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag
    13801 gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg
    13861 gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa
    13921 tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag
    13981 ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt
    14041 tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt
    14101 ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg
    14161 atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac
    14221 gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac
    14281 aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga
    14341 cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg
    14401 aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt
    14461 tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag
    14521 atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag
    14581 aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg
    14641 atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg
    14701 ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga
    14761 ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg
    14821 tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga
    14881 gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc
    14941 tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata
    15001 cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa
    15061 attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg
    15121 tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta
    15181 gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga
    15241 atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct
    15301 gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct
    15361 gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc
    15421 gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt
    15481 ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag
    15541 attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag
    15601 ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat
    15661 aaatacgcaa gaaatgcggc agcacatgct atgcatgacg ctatcagtaa atatattccg
    15721 gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca
    15781 ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg
    15841 ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg
    15901 gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa
    15961 tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg
    16021 acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt
    16081 tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac
    16141 tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc
    16201 tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc
    16261 tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc
    16321 gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt
    16381 catcaatact tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc
    16441 tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct
    16501 tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga
    16561 gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat
    16621 cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta
    16681 ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg
    16741 gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac
    16801 aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt
    16861 taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc
    16921 acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac
    16981 tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa
    17041 ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac
    17101 actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa
    17161 agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac
    17221 acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc
    17281 gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc
    17341 gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca
    17401 agacaagctt tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag
    17461 acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac
    17521 tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag
    17581 gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac
    17641 ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata
    17701 ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt
    17761 gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta
    17821 tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa
    17881 tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt
    17941 acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt
    18001 ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa
    18061 gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa
    18121 tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc
    18181 gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta
    18241 ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa
    18301 atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag
    18361 cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt
    18421 atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt
    18481 tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt
    18541 catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac
    18601 tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat
    18661 aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta
    18721 gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa
    18781 acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat
    18841 gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac
    18901 agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca
    18961 gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa
    19021 gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg
    19081 cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc
    19141 aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta
    19201 atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg
    19261 gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac
    19321 atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc
    19381 atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc
    19441 aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca
    19501 atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa
    19561 caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa
    19621 aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg
    19681 gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa
    19741 tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat
    19801 tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt
    19861 agcgatccac gatgcaatca aggcagggac agatgaacga gattactata ttgataacta
    19921 tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa
    19981 accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc
    20041 tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat
    20101 acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt
    20161 cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac
    20221 ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca
    20281 tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga
    20341 cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg
    20401 atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg
    20461 catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac
    20521 ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca
    20581 agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc
    20641 tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata
    20701 tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca
    20761 tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa
    20821 aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc
    20881 aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata
    20941 tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag
    21001 tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata
    21061 caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc
    21121 tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac
    21181 cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat
    21241 ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca
    21301 tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg
    21361 taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac
    21421 gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt
    21481 atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag
    21541 tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct
    21601 accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt
    21661 atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat
    21721 tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat
    21781 attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa
    21841 aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt
    21901 ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac
    21961 aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca
    22021 atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa
    22081 tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt
    22141 gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta
    22201 tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact
    22261 ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa
    22321 actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa
    22381 gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa
    22441 gacggttttt ttgtacgaaa aaattcattg gtcgttgaat gggctgaata gtagtgtata
    22501 aaacaatttt gtggagcgtg aagcttttat ggtagaggaa aatacgaata aagtgatgtt
    22561 gataaaaaga ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa tgagtttgac
    22621 caatcattat cctatcgaaa tttacagttt cgttcgattg gtctgtcaat taatgaaaaa
    22681 gtaaaaaaga atatgttcta ggaagagggc ggcacatcta aatttttaag taacggacag
    22741 ttcaaatatc aagcattgaa agaggctatc ctactgtttg aaggaatacg aatatataaa
    22801 aacgacaaag aggaaaaagt agttcattaa atcccttcaa gtgatgaaag aatgagatct
    22861 aataaaacct gatctttcat ctgttttttt aataacgtaa taattttttt attttttgtg
    22921 ctttcgccgt tattgtagag tgatttagca ttcttatttt taaaaataac tgtgttatat
    22981 attaaaaaag gcataatagt tataagcttt tttaaagaat atttagaaat tattaatttt
    23041 cgtctgaaaa caaaaaaaga aagcgttatt atttagtcta ttttcagggg gggaacctta
    23101 tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact
    23161 ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat
    23221 gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca
    23281 gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa
    23341 tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg
    23401 cggtactttt aaaccacgtc aggaaatgtt catttatgtc tagggaagtt ggcgatgaac
    23461 caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg
    23521 acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg
    23581 cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct
    23641 gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa
    23701 tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg
    23761 ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa
    23821 ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt
    23881 aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat
    23941 ttttcatgcc ataatcgcat agaaatgtct cctttatata tagattgtta accttagacg
    24001 ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct
    24061 gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca
    24121 atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt
    24181 aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga
    24241 caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata
    24301 gtagaatagt tataatgact atagaaagct acacatgcaa tcaatagtac tcaatgtttt
    24361 ttatttaaac gtatcaaata aagaatgacg atcaataaag acaaaaccag tcctgataat
    24421 tcttctataa aaacactatc gggttcaaag tagttgtaat taaaaacttt ccacataaag
    24481 tagatagccg gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca attcataaga
    24541 taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct caccaacgta
    24601 atcatgtaac ggactcctca acgattcata taataaaaga taacatgtta ttgcatacat
    24661 cacaaatgaa atgctaggaa aataaaagaa gaatagatca atgaaaaatg aaaaagcaga
    24721 acagttttct gctgataagc aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa
    24781 aatcaatgga ttgatatcta agtaattcaa tacatataaa aagaggacga tcgcattcac
    24841 taaaaaggaa agaatgatga tcccatagcg aatggaaaca tatttaacaa atacttttgt
    24901 ttgaatacgg gcatatcttt ttcctttata gctgagcatc atgacaagaa gcaaaagggt
    24961 aatcacacct tgaaaaatca ttccaataaa cagaattcct gaggtttgat aatttaattg
    25021 gagaaaataa gctgctgccg caactaaatc gaataaaatc gcaataaatg gcaaaataat
    25081 cactcctaac attgagaata aataaaagaa taacattttt tctaaaataa gcaaataaaa
    25141 attaaagtaa caaaatagtt atgacacaag taaaagaaat aagaataaca atattatttt
    25201 acttatattt tgaaacttgt gagagtttgt cattcgtgct atagttgaaa agtaacaaaa
    25261 ataaatttta tttatgatgt acctttagct cagttggtta gagcagacgg ctcataaccg
    25321 tccggtcgta ggttcgagtc ctacaaggta cataagaggt tgtgaaaaag ctgccctgca
    25381 tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttacttg gctgttctta
    25441 ttactgcaga tgcaagcttt tgaataccgt ttaaggaaga ggttgtgaaa tcagcgtttt
    25501 gacctgagta ttagcttaac aaacggaaaa atagttcctc atattttgcc gtttgttaag
    25561 ctaataccga gggtcagctg attgaacacc atttaagaaa gaggtcgtga gcctgccgtt
    25621 tcactctgtg tcataaaaaa gccaatctac tttgatagaa aaagcacgta ggactaaaac
    25681 agttctgcgt gcttttttta ttgtgtgtta aatatcgtaa atgaagatag atatttttat
    25741 gattcgacga tagagacgtt gaaatctttc aacagctcta aagcggattc tccgtgtttt
    25801 ctactggaga ctaacattgg atcaacaaaa aagtctgctt caggaaaagc actctgcagg
    25861 cctatcagat tagagagtac acacaagttt gtttcaatcc cgactacttc gatctctttt
    25921 tgttcttctt tgctttcaaa taaagtttcc tggatttcag ctaaagtttc aggtggaatc
    25981 gtaaagtaat actttttaat cactcgttca ttttcttgtg gagaaagaag agggatcaat
    26041 tgcaaatctt ctcgttcttc ttctttgtcc ttcagttcaa ctggtatatc ccgtgtgaag
    26101 agaatatatt cattattttg gcgtgcttta gctaatcttt gagcaatacg ttctgccaat
    26161 tgatctgact ctggaatata aaattcgctg tttggatcaa gaatatgatt ttgcatatca
    26221 ataataacta acattttctt tcctcctaca ttcatatcgt cattttattc tttttaatag
    26281 aagtttttta aaatataagc aacaccagct tcttcattcg tcagtgttac ttggtttgca
    26341 atttctttta cttgtcgctg tgcattcccc atcgcaatac tcaacccgct gtaattcaac
    26401 atagcaatgt cgttttcttg atcgccaaaa gccatgatgt cactcgattc gattccaagg
    26461 taatccgcca gaaaaacaag tgaatgagct ttagtgatac ctgtaggcgt gatttctaag
    26521 aaatgcggat cagagaatac tgattggata ccatctgctt gcagatctct ttgtagcttc
    26581 tgtagttttt cttcactctt atctgttaat gctaatttaa gatagctaag agaaggatct
    26641 tccaacatat ttaaaatttg atcaaaagaa tggttctgta aattgaaatg ttcataataa
    26701 gggtcatctt tgactagatt tgattcatcg aatgtcagct gatcatcttg ggtttctcgg
    26761 ctagcaatga tggatatttt cgcttcttga gcatagaaga aggcttttct cagtgtatcg
    26821 ctatctattt ttgaacgtct gagaactttc gccatttttc catccttcat ttcagcaatc
    26881 agtgaaccat tggaagaaat caaatagcca ccgattccta attcggaaaa aatatcttta
    26941 gcagaaatgc tgttccttcc ggtagcgacg gttacgattc cgccattttc atcaaatttc
    27001 cgaagtgctt ggatattttc ttctggtatc tttccatttc gatcaagcaa ggtaccgtct
    27061 aaatcaatcg ctgctagttt cattttatct tcctactttc tttttacata tctattttcc
    27121 tgttacctta agattaaatg agaatttaaa aaaatgaaac taacttgctt tgtcattatg
    27181 aattttttca aaaaacattc ttaagaaaaa ggtggctata tatagtagtt tttggtcaat
    27241 acctttgaat aaaaagccat gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat
    27301 aaaaaaattt taaaatattc aatgaaaaca aagcatatat tttgctttaa aaccaaatga
    27361 ggaatattct agatgtagaa caggttagaa aaagattaga aaaaggagtg attttgatgg
    27421 agaagagata cggcgtagtt cctgtggtaa ctgcttcaga cgaaaattat gctccttact
    27481 tgagcgtgat gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt
    27541 atgtgattga cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata
    27601 aatattcaag taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact
    27661 tcctagtcag tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat
    27721 tagctaaaga ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg
    27781 atatcgtaaa actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc
    27841 caggccaagt gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt
    27901 caggagtgat ggtgattgac attgatcaat ggaataaaaa agaaattaca gaaaaaacta
    27961 tccattacct cagtgaaaat ggcgatcgaa tcatctatca cgaccaagat gctttgaatg
    28021 ctgtacttta tgaagattgg gaacaacttc atccaaaatg gaacatgcag acttcgctga
    28081 tttttgaacg tcatccagca ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg
    28141 aaaaaccttc gatcgttcac tttacaggac atgataaacc ttggaatacg ttgaaagatc
    28201 atccgtatac caatttatac ttaaaaaaat tagcacacag tactctaacg aaagtaggcg
    28261 agatcaatga ataaaaaaga aatagcagta gtcgcaagtt gtaacacaaa attcgtacca
    28321 catttagctg cattatttgt ctcagtatta gataattgca acccgtctaa atttgttcgg
    28381 ttctatgtga ttgacgatga tatcgatttt gaaagtaaac agcttttacg cttctcagtg
    28441 aaaaacgcac gaatgaactc agatgtggaa ttcttgaaaa tcaataaaga attctttaca
    28501 aatgtggtaa tcagcgatcg tattccagaa acagcttatt atcgtattgc gattccggaa
    28561 ttgttccgcg gcacagaagt tgaacgtatc ttatatatgg attgcgacat gatcgcttta
    28621 caagatatct ctaaattatg gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa
    28681 gacgctgggt tccatcaacg actggaaaaa atgaaaattt cagctaaatc aatgcgttat
    28741 ttcaactcag gtttgatgct gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa
    28801 aaagtattag atttcatcga acataatcca gaaaaattac gattccatga ccaagatgca
    28861 ttaaatgcta tcttacatga ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc
    28921 tatatcatgg caaaagcaaa aaaacatcca acagcagcag gtgaacgtga atacgaagaa
    28981 actcgcaaca atccttacat tatccatttt tcaggacatg tgaaaccgtg gagcaaggat
    29041 tttgaagggc caacgaagaa atattacgaa aaatatgcag ggatgacggc tttccgttgt
    29101 gtagctaaat tccctaaata tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt
    29161 gttaaataat agaagacaac aactgaagag ggggagtagg aatggaaatt acaatgtcta
    29221 aaaatgcagt agagacgtta attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc
    29281 ttatcaatga ttccgatcca tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt
    29341 ttcaaatcat tccttttgtt tcggaatttg gagaatatgc gactaaaatc gagcatccat
    29401 tattggacat ctatacatct aaattagagc aaaattattt cggtaaacga ttgaacatgg
    29461 acttcaacaa acagcttgac agcttttcat tagaaaatga aatcgcagta ctcgattata
    29521 atatcaaatt aaagaactgc ttcttcagct aatagtttcg agaaagtcca aaaaggaggt
    29581 atacattgag atactacttt gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa
    29641 aatataattg gctcgtactt gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag
    29701 aattcgagct tcctgatgat attttcgtag gaaccgaata tccggaagaa gtttctcgga
    29761 tggagcgcct ttatgagacg aaactagagc atccattttc tttggtagtg attaatctag
    29821 atcagtcaga taatcgtatc gaaaaaaaat taacgccaat atcttttgta ctttccgata
    29881 atctcttgat tacttgtatc gatagaaaaa ccgaatttgt ggatcggctg ctgacaaagc
    29941 atggaaatca tttaaactcg tttgaaaaga tcatcacgta tacattatta gatatttaca
    30001 cccattacgt gaaagaacta agagagatga aaaagcgaat tgacgctttg gataaagcag
    30061 ctcgtaaaac aacggaaaat gaagaactgt ataaacaggc agatcttgaa agagacatcg
    30121 tatatatcga tcatacctta agagatcaac gggaaacaat ggatcaatta tggaattctg
    30181 aaactttcat caaacgttta gacgatgaac agctactcta tgatgtccat ttgagacagc
    30241 gacaaacaga aaaaatgatc gagatctatc gagatttatt agagagtatt ggtgatctat
    30301 tcaacgggat gatggacaat aatctaaatc atatcatgaa atatctagat tcagctgcat
    30361 taatcatttc cgttccggcg ttgttcgcag ggatttgggg aatgaataca ggaggattac
    30421 cgggtaaaag ttctgctcta ggctttatac tcgtagtctt tggctctttg atagcagcag
    30481 tagttttagg ttaccattta taccggaaag attatacaaa ttaaagaatc tatttaaaac
    30541 aggtatcaaa caaagaaatt tgttttggca atacaattta aaaactagga ggaatacagc
    30601 aatgacagaa ccaaaattaa tcgacccgcg aaacttgtac catacggaga aatttcctaa
    30661 acaagatcag gatacaccag ctttacagca gaatatgatt cctgttcctg attgtggaga
    30721 agaatcctat gaaggcaata atcaactaga aaaccgccgt gtactaatca ctggtggaga
    30781 ttcagggatc ggtcgtgcag cagcgattgc atttgctaga gaaggggcag atatcgctct
    30841 tcaattcttc ccaggagaag aaaaagatgc agaggaagta gcaaaatacg tgaaagttgc
    30901 aggacgtaag attgtcttac taccttttga tcttcgtgac gaacaagcac ctaaagaaat
    30961 catagataaa gccgtgagtg aacttggcgg gctagatacg atggtactaa acgcaggaca
    31021 gcaaatttct tgtcagtcta ttgctgagtt accacttgag caagtcaaag acacatttat
    31081 gataaacatc atcgctatgt ttgctttagt gaaagaagct gttccgcata tcccagcagg
    31141 aggagcaatc gtaacaacaa cttctgttca agcattcaat ccaagcgaac atttattaga
    31201 ttacgcagca acaaaagctt caatcgctaa ctttacgatc ggtttagcaa aacagcttgc
    31261 acctaaagga atccgtgtaa atggtgtagc acctggtcca atctggacac cattacaact
    31321 agacgaagga caaccttctg attcgatccc tgaatttggt cagcaaagtt tactagaacg
    31381 tgcaggtcag ccaggagaat tagcaccagt ttatgttttc ttagcatcga ataaagcaag
    31441 ttatgttaca gcccaaattt acggagtaac tggtggagaa gcgatcaact tataagaagt
    31501 tgatcgtttc acccctatga ggaggtgcga aatgaataaa ggtgtaaaaa cgatcggagt
    31561 gatcggaaca ttacttttgc tatctgtgct tttgtttgcg atactattaa gtgcaaccta
    31621 tccattgcct tttcagctag agaagtttcg attcttttcc atcactaatt catacgtgca
    31681 acaatatgta ttttggcttg ccgttgcatt tgctattttg acaattatca cgttacttgt
    31741 tttcctgttt tatccaaaaa caatcggaaa ctttctactg aaacatggtg atggggagtt
    31801 gacgttggat aaaaaagcaa ttgaaggaat ggttcgttct cacttgcatg aagatgaatt
    31861 catccattca ccaaaagtaa atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa
    31921 aggggagttg aaacgaactt cagcactcat tggtaaaaca ggaacattga tgaaagaaat
    31981 cgaaaatgaa gtacaacaca tattgggaac aaaagagcca gtgaaagtat cggtgaaata
    32041 cacaggcttt gaagaaccag ctaaacgaag caatgaccgc aaacgagtag aatagggggg
    32101 gaccaaaaat gaaagaacta ttttcgacat acaagctccc tatcatcttt ggggtacttg
    32161 gattgatatt agccgtttta tggatcaccg tgggcttttt caagacctta ttgatcttga
    32221 tcatggcagc gatcggcgtc gctatcggtt tttatctgaa acaaacgaaa atcttagatc
    32281 actattttaa atcttaggag gaaacatcca tgaataacaa taacaaaaat aataacaata
    32341 aaatgaacaa ccaaaaacca gtgtctgcac atccagaccc aacgacacca aaccaatcaa
    32401 atggtgtgaa cgatgaaaag aacaaaacag caccagtatc tgcacatcca gatccagtag
    32461 tccctaacgt tcaacaagga gtagaagaga agaaacatga tgcagaaaag catgcacctc
    32521 atcatccagg aactccagaa tctaaagttc cagaacaaca tgatgcaatc gatccttcta
    32581 aacctgtgga agagcaaaaa catccttctg accatgcgaa agcacaagaa aaacttagtt
    32641 ctgaccacac aaacggacaa ggaaaacttg gttctgatca tacaaataac caaggaaagc
    32701 atgattctga acaggaagat tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc
    32761 aaaaaatcat cggaatcgca attgaacaag tagacggttt attatctgca aacggtggtt
    32821 tcttctctaa tgtggcagga aaacttgtga atacagataa tgtaacggct gggatcgaaa
    32881 cagaagtcgg taaaaaacaa gtagcagttg atatggatgt cattgtggaa tatggcaaag
    32941 atatcgaaaa aatctttgaa gaaatgcaag aagtaatcgg caaagaagta aaaaatatga
    33001 cacatttaga agtgatcgaa gtcaacgcta acgtagtgga catcaaaaca aaagaagaat
    33061 ttgaaaaaga tcaagaaacg gtgcaagaca aagtaactga cgcagcgaaa aaaaacggtg
    33121 aatttgcttc gaatcaaaca gataaagtaa aatcagcagc tggaagcggc gcacaaaaag
    33181 taaaagaaaa tacagaacca cgggttcagt aataaggaag taaaacatta ggaggaacga
    33241 aacatgggat ttatttggtc acttatcgtc ggaggaatta tcggagcaat tgcaggagca
    33301 atcacgaata aaggttcttc gatggggatt attgcaaatg tcgtagcagg tttagttgga
    33361 tcagcacttg gtcaagcatt attaggttct tggggaccaa gtattgcagg aatggcaatc
    33421 gtgccatcat tgattggtgc catcatttta gtactaatcg tatcattctt tgctcgaaaa
    33481 ctgtaaaaag acttttaaaa agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa
    33541 cagccaaaca aaaatagctt ctcatatttt tcatttggtt gggcatatta tcgaagatgc
    33601 aagcttttga acactgttta tacggaataa gaggctgtga caagactttt gtcacagcct
    33661 cttatttatg cgggcaaatt cccaattaga tttttaaaag tttttcctta aatgtgtctt
    33721 tattgataca gagaagcttc tttaataaaa caatgcctcc ctaaattttc ttgagatagg
    33781 aaaggtgaac atacaaaata atacgaccac tttgaaaacc cagatgatcc acaaatggat
    33841 cgtatgatac tgatgaagta aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac
    33901 aaggaagaaa taatatctct tttttactct tgtctgtttt agccaataat agctcgtaaa
    33961 gtaatctaga caaacaaata gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg
    34021 ttgctctgct agacgattga cgagcaggaa aaatgttggt aatggaatat agatcacgag
    34081 taatgaaaga aaaaagtaaa caataaatgg catactattc ctcctaattg tgtgagttcg
    34141 ccctcatttt aaaataacga tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta
    34201 tctaatgtat ttttaaataa ataatcatta ttatcaaaat aat
//
LOCUS       DACDLI010000025        33270 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDLI010000025 DACDLI010000000
VERSION     DACDLI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.025"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1483)
                     /locus_tag="IV353_001223"
     CDS             complement(<1..1483)
                     /locus_tag="IV353_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9009488.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYN"
     gene            complement(1539..4019)
                     /locus_tag="IV353_001224"
     CDS             complement(1539..4019)
                     /locus_tag="IV353_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009489.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(4003..4338)
                     /locus_tag="IV353_001225"
                     /pseudo
     CDS             complement(4003..4338)
                     /locus_tag="IV353_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4352..5386)
                     /locus_tag="IV353_001226"
     CDS             complement(4352..5386)
                     /locus_tag="IV353_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9009490.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(5476..6156)
                     /locus_tag="IV353_001227"
     CDS             complement(5476..6156)
                     /locus_tag="IV353_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9009491.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(6440..7543)
                     /locus_tag="IV353_001228"
     CDS             complement(6440..7543)
                     /locus_tag="IV353_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9009492.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRAPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(8172..8642)
                     /locus_tag="IV353_001229"
     CDS             complement(8172..8642)
                     /locus_tag="IV353_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9009493.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            8972..10612
                     /locus_tag="IV353_001230"
     CDS             8972..10612
                     /locus_tag="IV353_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9009494.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            10766..12088
                     /locus_tag="IV353_001231"
     CDS             10766..12088
                     /locus_tag="IV353_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9009495.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(12156..12590)
                     /locus_tag="IV353_001232"
     CDS             complement(12156..12590)
                     /locus_tag="IV353_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9009496.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(12655..13491)
                     /locus_tag="IV353_001233"
     CDS             complement(12655..13491)
                     /locus_tag="IV353_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9009497.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(14233..14859)
                     /locus_tag="IV353_001234"
     CDS             complement(14233..14859)
                     /locus_tag="IV353_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009498.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(15154..15489)
                     /locus_tag="IV353_001235"
     CDS             complement(15154..15489)
                     /locus_tag="IV353_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9009499.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(15476..15760)
                     /locus_tag="IV353_001236"
     CDS             complement(15476..15760)
                     /locus_tag="IV353_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9009500.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(15816..17339)
                     /locus_tag="IV353_001237"
     CDS             complement(15816..17339)
                     /locus_tag="IV353_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9009501.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(17332..18507)
                     /locus_tag="IV353_001238"
     CDS             complement(17332..18507)
                     /locus_tag="IV353_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9009502.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(18511..18696)
                     /locus_tag="IV353_001239"
     CDS             complement(18511..18696)
                     /locus_tag="IV353_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009503.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(18662..20356)
                     /locus_tag="IV353_001240"
     CDS             complement(18662..20356)
                     /locus_tag="IV353_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9009504.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(20353..20826)
                     /locus_tag="IV353_001241"
     CDS             complement(20353..20826)
                     /locus_tag="IV353_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9009505.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(20895..21050)
                     /locus_tag="IV353_001242"
     CDS             complement(20895..21050)
                     /locus_tag="IV353_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9009506.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(21184..21564)
                     /locus_tag="IV353_001243"
     CDS             complement(21184..21564)
                     /locus_tag="IV353_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9009507.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(21568..21783)
                     /locus_tag="IV353_001244"
     CDS             complement(21568..21783)
                     /locus_tag="IV353_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009508.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(21786..22193)
                     /locus_tag="IV353_001245"
     CDS             complement(21786..22193)
                     /locus_tag="IV353_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9009509.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(22459..23934)
                     /locus_tag="IV353_001246"
     CDS             complement(22459..23934)
                     /locus_tag="IV353_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9009510.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(23924..24772)
                     /locus_tag="IV353_001247"
     CDS             complement(23924..24772)
                     /locus_tag="IV353_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9009511.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(24809..25171)
                     /locus_tag="IV353_001248"
     CDS             complement(24809..25171)
                     /locus_tag="IV353_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009512.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(25471..26095)
                     /locus_tag="IV353_001249"
                     /pseudo
     CDS             complement(25471..26095)
                     /locus_tag="IV353_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(26092..26403)
                     /locus_tag="IV353_001250"
     CDS             complement(26092..26403)
                     /locus_tag="IV353_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009513.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(26447..26740)
                     /locus_tag="IV353_001251"
     CDS             complement(26447..26740)
                     /locus_tag="IV353_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009514.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26934..27581
                     /locus_tag="IV353_001252"
     CDS             26934..27581
                     /locus_tag="IV353_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9009515.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            27642..28787
                     /locus_tag="IV353_001253"
     CDS             27642..28787
                     /locus_tag="IV353_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9009516.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(29536..29709)
                     /locus_tag="IV353_001254"
     CDS             complement(29536..29709)
                     /locus_tag="IV353_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009517.1"
                     /translation="MDKKNQELVKLMKAVYYNEYEKKQENHDSKVYYKELESSNAVFS
                     KDPVHNVVLCLEG"
     gene            complement(29741..31300)
                     /locus_tag="IV353_001255"
     CDS             complement(29741..31300)
                     /locus_tag="IV353_001255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009518.1"
                     /translation="MEGLLDDLGNFIEQMEAVDSYINSIDEIMGSTFQLKVILNEEEQ
                     SILDSFNLLNLMYIEMKENEARFIDNLKNINEKSNGRLFNELGKPEEGAPDELLKKLL
                     KMISSEERGVLFELSEDDSISLSLKDSKMRSKIEIYLEESRKRTRQRHLLFETSLITI
                     SNAFESLHSKLISFIIVNSSSSKINDKQLSFEQIIELSSLEEAKEYLIEKSVEDVMRG
                     SQITWLKYIGKNTFKEFFKELVDEDEEKFKEFFLRRNLIIHNNAVINKRYLNSIKKIS
                     DEKKAFLVGKKISVSLEYLEENMNIIFLISIKSVFYVVKKFFPTQFNIFFKKFHNIAF
                     NYLKSEKYEIAYSVFDMLWKDASKIDASNKLLLTVNYLQSLKWTGNLKRMEQIRKSED
                     FSLAKNEHLMCLALIDDNFKVAIDILEKLIAETSQSGKDDVALVNEYLTWPIFKEFIE
                     TTELNEFLKSKGYIEIKNQKEIIDEKTTVCHNKKRAVKYKSNFEVRKSVSSKIRRYRT
                     EANKSYSYFFR"
     gene            complement(31434..31679)
                     /locus_tag="IV353_001256"
     CDS             complement(31434..31679)
                     /locus_tag="IV353_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9009519.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            31784..32557
                     /locus_tag="IV353_001257"
     CDS             31784..32557
                     /locus_tag="IV353_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009520.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKSYDHPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(32610..32924)
                     /locus_tag="IV353_001258"
     CDS             complement(32610..32924)
                     /locus_tag="IV353_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009521.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT         9852 a         6639 c         5449 g        11330 t
ORIGIN      
        1 aattatattc gttgctagtg aatacaaaaa ctttagcgga cttctgtgac gggtactctt
       61 tcaattggct agtcaaaatg atatcatact gcttttcttt ctcgtattcg actaaatcat
      121 atttgatcgt attcagcaat tgttcctgga tgatttcttt cacgatatcc gccattagcc
      181 tttcataagg aagatcataa gcaatttgca gacgtaattc taactgcttg atcagcgtcc
      241 gcaaaataga tgcatagcga gcaaataatt caaagcgggc attttcctcg tctacttcag
      301 taaaatcaac aatagcaaat gtttgtctga ccaatctttc acataaaacc aggaattgtt
      361 tatcttctac ttcagttaac cgctgtttta atccttcttg gccaaaaata ttcatccatc
      421 ctctaaaata ggcaaaatga gcgtgatttt gcatcactgt caacgcaatc ttttgcgaaa
      481 tctcttctgg aaaaacataa aaagagaaat attcttctat cgattgttta aacgttcgat
      541 tcaattgagc tactacgtcg tctttgtatt tttcgcttat caaaaactcc tggacatgcg
      601 agttatgtgt atctaatgta aagttactga tcatgaaaag ataaaaaacc aacgtttcat
      661 tttgattcca aggaaaggca tattgaaaga agtaacgggt caaaaatgta tggaggtcca
      721 ataaaaattg tgtcgtttga aacaatccaa caggaagatt gctataatct ggtttggatt
      781 ttttcgtaca gcgcattcgc ttcttcataa tggaaagcca tatttcaaat ttcaatcgtc
      841 cttcttctgt aatagtaaaa ctaaactctt tttgcagcag atctaaaaaa gtgtttcctg
      901 ataaatcact gtattctttt ttgttttcaa gataaggacg tccaaaccaa aataattgga
      961 aataaaaata acaaatttga ctttctggac cggtcagccg cccgcttttt attttcagat
     1021 gaaactcatt taaagaatca tttaattcct tcactctgcg agacaaagta gccgaactga
     1081 tattaagttc catgacaagt ttctgccgat caaatgagcc attttccaac agataaagaa
     1141 gtatctgata ttttgtagaa tagcgcaagt agcaatgata ataaacatcg actgctgcta
     1201 tatttccatg tctgattaat aaaatattcc cttcttggat aacaatttcc aattgtttga
     1261 tattttccaa gttagcttgt aaataaaaca gatcttcttt caaggtagcc cgatcaactg
     1321 ataagcgggt aagtacgtta ttaacggaaa tctctccttt ttcctctaat aaaagttgga
     1381 aaagtttgat ttgccgttgt tcattttttt caagcaaact gaaaagttcc aagtccactc
     1441 ctccaagcat ttctgatttt aacagcagaa gtttccttct catccttgaa aaagaggatc
     1501 tgtgacagtt atcacagatc catacaaatt ctaatcactt agctggttgg cccagctgtc
     1561 agttcccatg tgaccatgcc ttgatacgtt gcgccaggtt gagcattcgt actgctattt
     1621 acctctaaga gtaaaccatc ggtatcggga aagtcaacga gaacctctcc tttagctgtt
     1681 ccatcactct cataaatcaa cgtgttttga ccttgctgaa tcaattgttt tgatccagaa
     1741 tgattgaagt ataattttgt tccgcttaat tcttgacctt gatcattctt aaataaagaa
     1801 gtcaatgtcc ctgtcagttg ccaaggagac gtgcttggaa gccgagtatc ccgcacatca
     1861 aaagaaaaat cgccttttgc ttcataataa cttgtcttca acggtttggg gagatcgtta
     1921 ccaaatgtaa aatcatctgg tacattaact aatgttaatt cttgtttggg gacaagaatt
     1981 gttttagcag atgtttggac aactgggtta ggaagcttag caccatctgc taatgtaata
     2041 ttgctggaaa gtacagctgc ctttgtcagt atgctgacgg attgtacaga agtatctaca
     2101 gtatttaatt gatagttgta agtaagatga atggtatctc ctattttgct caatagatct
     2161 gctgggacag aaactccttg ttgaatgccg gtatctgcga tagttgtacc atttaatttt
     2221 aaggaaccat tgatataagt agtattttca ggaagtgaga tcgtgatttt tttgtcggtt
     2281 atcgcatccc cagattttgt taacgtgaat gtcacatttt cttgaatcgt atctcctgat
     2341 agagcagaca ggggatctcc tgtctcaggt tgtctgctaa ggttttttag ctgactatct
     2401 gcactcacag atggaaaatt agccactgtt atttgaggaa gagtggtgat tccagcttgt
     2461 tcagactgcc atttgtacgc aaaagacgga tacatgttag taagtacatc gtccaccaag
     2521 cgataatcaa aataatattg ccctgaagcc aatgcaggta gtgtagaagt agctgtattt
     2581 acacccccgt tatttgattg gatcgtttgg aaaagttgat ctgtccatcc tgtcgctaat
     2641 ttttgggttc ctttcgaata aagcctgtaa tggatcgaac ccttttttag ttcagttgct
     2701 gaccaattgg ccgtgacttg gtacgatgca gaagaaagga gctttcccca ctgactatcg
     2761 gctgttaagt ttgttggtaa aaccgttccg tctagcttgt actgctccaa tacctttaaa
     2821 gcaacaggag aaagcaatcc gccttgcgca tctttcccac tgtacacgat attggctgtc
     2881 tgattcaatc cacttgtgct tgctggagta tgagtcagta ttgctccttg tgtaacgttt
     2941 tttcctgtaa aatagttgac tacgcgatca aagcgactaa atggcaactg atcaaaacgt
     3001 ccttcagtca gccccatcgt caatgtccga ctattggtag agcctggaag gttctggtta
     3061 tttccatcca cgtattctgt ggtatcagca aatgtattat attttcctgt tgaatcactt
     3121 gttgagatgt aactattcca cgtatttatc tctgcacttg cagaaaatgc atatgctcct
     3181 ggattcaagt ttccgccagt taatttagga taaaatctac ctgcagctgg agctaaaatc
     3241 gaattgaaca tacgatcttg gataatatta ttcgaatcat aaaaatcaac agaaaaaacg
     3301 gcctttttag acatagaagg ttgtaattta cccttggtca aaattttgtt tggactcgta
     3361 tcatatttat ctccgttttc tgtcatcttc gcgacattag ctgtaacatc aaattgaagc
     3421 gggatatcgt ccacgttgaa ttcgcctcgc gtaataatag caataatatc tgccaataga
     3481 gtatttgtat cccacgaccc catcccaaaa cgcaaggtat ggcttcctgc tgataataaa
     3541 ctaccgctag taaccttgat gggggcaccg gcaatataca agtaggcgtt gttccagtca
     3601 attgctttta atacatcact gcttgataca tgttccggca aattgatgat cgccacaaat
     3661 gcaaatcctt gtgcagtgat attcaccaaa tcaagtacat ttgttagttt cacatggctt
     3721 tcccaatgaa aacggaccgt gccacctttc caaggatcat ttgaaccatc gttcaaataa
     3781 gtagcagtca atttaggcaa ttggttagcc ccagttagtt ggacaagctt cccgccattg
     3841 actgcactac gtccaccatt tggaggtacg atattttcat acgtgtatgg atcataagaa
     3901 atggcaggag gcgttgctcc aacagcttct gccgaaggtg aaaagataaa tatacttaaa
     3961 acaaaaagcg aagtaaaaaa gaaggagcga aaaaatgaaa atttattttg tagcaacatg
     4021 ttcatcctcc ttttcctttc ttctccataa caacaaccac gcaagaagcc ataattctat
     4081 accaatgatc aaataaagaa aacggatctt ttcttcattc gtctccggga atacgccatg
     4141 ttgattagat tctttataaa tcggtagctg tgtctgctat tttgatgcgc cattgactga
     4201 accacttgga tgcggaactg gacttagatc ataaaatgta atcgtacctg aacttttggt
     4261 atctgcttgg actggctctt gccagctgaa gaacagcaaa cttgcaataa tccctcccaa
     4321 taagatcgat gttttcaacg aaatcacctc atcatgttgt ttctcctttt ttctttttct
     4381 tgtggaccca caaaaacagc aaaatcaacc ataacactag aactgcagca accaatcctg
     4441 cataaagcca tgggaaagat tgttcttgta ctgcaaaaac ggaattttca tttacttttt
     4501 gtgctttttc ttttttgatc gtaaagtttt gtgtaaatcg ccattcatta ctgtcgcctg
     4561 attttgctat gatttctgcg cggtaatcgc cggcttttgc tttatcacca gctaaatcaa
     4621 gattcaagtg aaaattcgaa tttggtgcaa ctaaaaattc attagacgta ttttcaatat
     4681 aaagctgatt ttcataaaag atcttgctcg taaatatcaa attgctgata atcctaggct
     4741 gaggattttg gaaacgaagc tgaacagttg gctttgcgtt gacctcgtcc aaatctcctg
     4801 ataaaagttt cagatccggt tctatcgtct cttgactttc ttgtaaaacg actgcgagat
     4861 tataagcaat acgatttttg atttgtgctc cttctgtagc attttctgtt tcagcttctt
     4921 ctttaggtga aacatgaata cccgctaata cacgtccctt aaagcttttt gctgggattt
     4981 tcacttctat aggtatagtt gtttctgcat gtgctgatac attgatttcc tttttcgcaa
     5041 tgagcaaaac gtcacggaca tcaaacggca atgtctcatc taacgttggg tttttttgac
     5101 tataatcgat cgttccacca tcactcgtta ccgccggatt gacagagacc tcaaatgttc
     5161 gttcttcatc tgaagaattg ctgataatta cttctagtgt ctgattttgt tccggtgcca
     5221 ctaataaatc aaaataaccg attgctttat ctatttggtt ctctggaaat accggtttga
     5281 ttccaaagtc tcctgctgaa tcctttgctt ggctgattga cggaaaaatg aaaaatagac
     5341 ttaaaataga aaaaaacgaa acaaacagtc gtttactttg attcattgtc caccacatcc
     5401 tttcaacaac aactgcggta aaaaatcctt cacctatagt attttcaccg cagtttgttg
     5461 aatttatttc atactctatg ctactgggcc agccacgagg ttccaagtca aatcagccgt
     5521 gaatgttcca gctgctgctt tcggcacttt cagtgtgata tttgctggat cataaacaga
     5581 aagccatgtt cccagacctt gatcttttgc tgctgtaaat attggtgtcg ctgcacttgc
     5641 tttactcaac ccagttacag tttgactagt tggtccagtg gattcgttat ctgacgtact
     5701 tttaacagtg gatgttccaa aatctaattc agcgccttgc aaaacttgtg aaccgtttac
     5761 aaaatctgaa accgaaactg tcacgttcca cccttgtgct tggccatctg cacctacacc
     5821 tcgacgatct gatacttgga gataaggtgt atcgtttttt gaagcgtcta cttgatacgt
     5881 ttttgttccg ctttcaatct cgtgtgtacc aaatggcaat tcaggaacta catccaatgt
     5941 taaagaacct gtctggccag ttgctggatt ttccggatct gctggctcta ctggttttga
     6001 aggatcagta gggttaagag gctcaacggt atttgttggt tcagtaaagg taatatctcc
     6061 ttttgaagtc gcttgaccct cagcaaaagc tggtgttgcc aagccgccta gaagaagtgt
     6121 tgaaagtaat aatgtcccaa tcatgttctt tttcataata aatcactcct ttttcttagt
     6181 gacctaagta tagtagagac agtatgcaaa aaatcctcat gaattcatcc gcttacttga
     6241 aattatgacc ctttttgtat aaaagagaaa aaaacaataa aatcgtttac atgaatattc
     6301 ttcctttttt attaagtatg aaaaaatatt tcaattttat tttttcgttt caaattattc
     6361 tattagtgac atccttgaac tataaaaata ggagtacggc atcatctgcc gcactcctat
     6421 ttttttcgtc atttatttct taaataaaac gattctcacg caattttatt tttttgatat
     6481 cttctactgt ttgtgttcca gctaattgca tgaccatttc caattctgtt ttaaagaaat
     6541 caaacacttg atggactcct gtacttcctc ctaaagacaa tccataaata acgggtctgc
     6601 caatagcaac aagatcggca cctgatgcga ttgctttgaa aacatgttgt ccacgtcgta
     6661 caccgctgtc aaacacgatt ggtgcacgtt tatctactgc ttctgccaca tattgcaacg
     6721 aatcaaacgc agcaggcccc ccatctaatt gacggccacc atggttagat acccaaattc
     6781 caccagcacc agattctaat gatcgataga catcttcttc tgattgaaca cctttcacgt
     6841 agactggcaa atcagaatga gcagcgataa attctacatc ttttggacta agtttttgtt
     6901 tggaagattt atatacagca tccatcgttt ggcctactcc tgattgataa gcctgaacga
     6961 tcggcattgg cagcgggaag gtaaacccgt ttctgcggtc tgtttcacgg tttccgccaa
     7021 ctgttgcatc cgctgtcaat acgattgctt ttgctccatt acgcttggcc acttcaagaa
     7081 tatccaaatt gattccatta tctttgctca tataaaattg aaaccattgc ggtgctttct
     7141 ccccgcctgc ttctcttatc tcttctaaag tacaagaagc gtaagaactt gctgtataga
     7201 tcgtcccaaa atctgctaca ccttttgcag aagctttctc cgccttcaca tgagctaatc
     7261 catgagcagc aactggtgcc atgatgattg gtgcagtcag catttcttcg tcaaaatgtg
     7321 tagttgtatc gggtaattct acatctctta atacatgagg aataatcaaa cgatgattga
     7381 atgcccgttc attttcttga taagtaaata agtcgccagc accactgctg atgtaaccat
     7441 aaccaccagc tggaatcact tgtgaagccg cgatttctaa atcttccata ttgataaaat
     7501 ctactattcc ttcatttgta cctgcttgat atgttttttc catctcaatg ccttctctct
     7561 ataaaatttg aatgatgaac aatatgatag cgtttacact ttttatatta ctcttcgaca
     7621 ttgatacagg caaataatct gtttacattt cagccattcc agatgtcact actctggaag
     7681 aaatgattgt aataaaaata gataaaaaac cgatgggact ttgacaaaag tagtgtcaaa
     7741 gtcccttttt ttgaataaac ggtgttcaaa agccattttc tcggtattaa gtcaaattta
     7801 tgcaaaaatg tggaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact
     7861 ttttcacaac ctctcgataa aacggtgttc aatcagctga ccctcggtat tagctcaaaa
     7921 aatggcaaaa tatgaggaac tattttgcca tttgtctctc taatactcag gtcagaacgt
     7981 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
     8041 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
     8101 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
     8161 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
     8221 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
     8281 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
     8341 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
     8401 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
     8461 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
     8521 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
     8581 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
     8641 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
     8701 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
     8761 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
     8821 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
     8881 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
     8941 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     9001 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     9061 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     9121 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     9181 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     9241 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     9301 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     9361 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     9421 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     9481 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     9541 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     9601 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     9661 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     9721 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     9781 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     9841 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     9901 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     9961 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
    10021 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
    10081 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
    10141 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
    10201 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
    10261 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
    10321 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
    10381 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
    10441 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
    10501 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
    10561 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
    10621 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
    10681 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
    10741 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
    10801 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
    10861 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
    10921 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
    10981 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
    11041 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
    11101 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
    11161 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
    11221 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
    11281 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
    11341 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
    11401 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
    11461 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
    11521 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
    11581 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
    11641 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
    11701 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
    11761 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
    11821 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
    11881 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
    11941 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
    12001 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
    12061 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
    12121 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
    12181 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
    12241 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
    12301 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
    12361 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
    12421 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
    12481 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
    12541 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
    12601 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
    12661 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
    12721 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
    12781 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
    12841 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
    12901 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
    12961 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
    13021 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
    13081 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
    13141 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
    13201 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
    13261 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
    13321 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
    13381 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
    13441 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
    13501 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
    13561 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
    13621 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
    13681 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
    13741 agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca
    13801 gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt
    13861 ttaaggaaga gactgtgaag tcaacgtttt gacctgatta ttagagaaac aaatggcaaa
    13921 atagttcttc atatttcgcc gtttttcgag cgaatgccga ggggcagctg attgaacatc
    13981 gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac aatccctttt tttatatctt
    14041 attaattcat tcttttgttc tattcttctt cttccgccat gaatgagaaa cccatatcaa
    14101 agaactataa aggttatcaa aacagcgttt atgactaaat gaattagagt tatctatcac
    14161 tatttttcaa gcgatttaaa ccaaatggca tacaatatgg catacaaaaa cttcaataag
    14221 ccgtgggggt ttttaaattt cttcttgttc tttaaaatca atgagtggag ctacataatt
    14281 tgaatccact ttttcaaacg tcactttttg catcgacgaa tgttttcttt cccaagataa
    14341 cgtaaataga tttgactctg gacttataaa gtctacttca ccttctagta ttactttctt
    14401 atgcaaatat tcctctaata aatatagact tgctaaagca tacaacacat tttccaaatt
    14461 tgcttcctta taatttgaag aacgaccatg ttttactctg ttatatattt tccaccaatc
    14521 tggtgtatct tcaggccacc catctccaaa aggttttagt gaagtcaatg ttaaagaatt
    14581 cataacccta acaccattat ccaaacacat taattcgcta gatttaataa cagctttaaa
    14641 atcagttatg cttttattgc tactgctatc aaatccgcaa agttctttga aaataatctc
    14701 acactccgaa ccaatcgcta gtagtagttt ttgatattca atagagaatg ttttgaaatt
    14761 attctcatcg agcatcacaa aatctgtggt attaataaac tcattttcta aatgtagata
    14821 atacttccaa taaaaatttg taaactctgc tttgtccatt aaatcacctc ttttgaatta
    14881 taacaaaaaa aacacgtcca caacagacgt gttctttgat ttctacacaa tcagcttgcc
    14941 aaaaccatcc aatcagtcct agcatgcctg tattaaaatt gaacgttcat catctatcat
    15001 ctttcttagt ccagcatgtt tgcaaccaca cctacaattt tcagtccaac aatagatttc
    15061 gactagctaa gtcttgcaaa attcaggcag ctaacccttc atttactacc ttaattatac
    15121 taaataccca caacgtttac aagtgtaatc attctaagaa atctgtttag ctatgatcgt
    15181 tgtataatct ttaaacgccg ttccttcatt gattgaaata attttataac tcttgttttt
    15241 ccaagctact ttcatatcgt ttgttagttc ataatcttga tcgtatcgaa taataaacgt
    15301 caacgttcct tcaagaatgg tacctacact agcaagcacg tcttttagca tttgtgaacg
    15361 cacattagcc caacattcaa atacagtttc ttcactatcc accatctgcc catcttcatc
    15421 ttttacccgt tttgtctttt taaacttgac tctctcagtc aattcattta cattcttaat
    15481 gattggcata ataagcacct ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc
    15541 gttcgctgct ggcttcttga cggttcaaat accaatgttg agtaagtaat gacaccgcca
    15601 aatcaaattg ctgataatta tccatatctt tatccgttgc gctactatca atagcacttt
    15661 ttatatattc gcctgctgta tcaatcaatt gttgaataaa gtcatcatca atagtgtgat
    15721 cgatccgcat actttttttt atgctatcca aaattaccat tttatctctc ctaatgaaaa
    15781 aaaggcagtg ggataaaccg ccgcccaata tttttctata cttttggtgt tacaactggc
    15841 gtaaactcaa tatacactga agcattttga tcaattttct tataatcgtt gcgaacgatc
    15901 actgcaagtc cttgtgagta gtaatcgaat ttttcccatt gagtcgttac ttgatttcta
    15961 cgagcaacaa agatagattg tgcaatatcc cccataatca tagggaatgt tccagcttta
    16021 ggattggcaa acaatgtatc tgcaatcaat actactggca taccgaataa tgatttacca
    16081 ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat ctgaatcttt caatgtatct
    16141 aggtggtaat atccgctttg gtttagaatt accattttat ttaatgctgg gtctaatgcc
    16201 acattgtata gttgtttcaa atcgtccaac gtagcagccg ttttcttagt gaatgttttt
    16261 aataaatcca tgatatgctt attgtcagtg ttttctacca atttcgctaa ctgatctttg
    16321 acctcttgta caatattcac tgctgaatct tccacaacct catttgataa ggcaatctta
    16381 ccagcacgag tttctacttt ataatcaact gaagtaaaca tttcagcgtc aatatcacca
    16441 atttcagcaa gttcagcttt tgttgctaac actgcttgtt ggttagttgc tactggatat
    16501 ttaccttgtc catttgatac tgtttttact gttgcatatt gagctaagtt ataatttgaa
    16561 cgtttcaaat caaagacttc accgattact tcttcgggaa caactgccgc tgcatttaca
    16621 gtagtgactc catcacgcac ttcgccttgt gaacggatat attcttcata cccacgcaat
    16681 tctgtttttt cgttatcgat taaagttttt tccatgttat tactctcctt ttgtgcttta
    16741 gaagtggatt ctaagctatt gtttgtttgt tttgcttggt tgttactcat aaacgattcg
    16801 taagaacgct tatttacttg gacatttgat gaatcataag cgggaacggt cacgacacta
    16861 atttcattaa gtgctttgat tttatctatt gatcgttcaa ttgtgccatc ttcttttttg
    16921 tcgaatgaat ctaatccaag cacgaaccca aaggacatag aatcaacaac cccttttgag
    16981 atattctcat acacatcatt tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat
    17041 cctgtatcat cgacattcaa tttcaacgta cctgctttga cactagctag tggctttgaa
    17101 taatcgtgtc catataataa gaacacattt gataaatcca cctcttttaa tgcttcgggt
    17161 gtaatcactt ctacaaagcc gcctaaatct tttgacggtt gcccgaattt caacgcatag
    17221 cctgaaatag ttttgccgtc tgttggttgt ttttcatttt ctttctctac tttgggagaa
    17281 tcggctgtta gctcagcttc ttctgtcagc cgtttttcct tttcttcttc attattcaac
    17341 tggtgtaact ccttcctgtt cttttaattg atatttctcc aacgtatcta aatacgtaaa
    17401 gttcaaactt gctagtaaac gatcgccacc atccatgggg ggtagcccca ttttcgaccg
    17461 cccctcatta atcgtgagaa gtgacccttt gacctgatcc aatacatttt ttacttttgt
    17521 ttcaggatca gtctctagta actgatcgga attaaagcgg atattggttt caagtttcgt
    17581 atctaattca ctcacaaaca cattaaagta atgaatcagc gtactttgga tatactggag
    17641 attactttga acggtgctag aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc
    17701 aaacgccttg gcaatttgtt ttgtgttcca atcattggaa ttgataagct tcaaaacatc
    17761 ggtgtttatt tctaatgttt tgtagtccat tgtttcatca agaatgatcg ttctaagagc
    17821 attatctcca ctacttgaac cgttggcttc ttcaaacttt tcacgaattg cgttctttgc
    17881 tgatccgtct aaatctgact tatgaacctt caaaatacct gacccactga caccacgagt
    17941 aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc
    18001 ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc
    18061 gctagacttc acacgtcttt ttttatcacc aatctgataa gacaacgtac cattatctaa
    18121 ttgagtaacc gttacacttg agttaggtaa taagtgaatt tcttctacct tttcaccgtt
    18181 gcgtttaatc tctgcaaagc tattaccgtt tagcaacata ttgaccgcca aagcaaactt
    18241 taaatgccaa ccatccattt ctgaattagg tttctcgttt agtaacttca ccagttcatt
    18301 atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc
    18361 aataattcga acggctgcaa acacatcact atttcttaat gcacgaacgc ttgtaaagct
    18421 ggtggtgtaa tcatccgatt ggattgatac cacatgttgt aggaaaggat ctcccgttgt
    18481 attttgtctc gctttaaaaa atgccatctt ttaacctccc ttctgttctg tatttagcat
    18541 gaccgctacc ccaataagca ctatgcccag cgctaacaag ccatagtaga catttgttag
    18601 taaggtgata gcaaccaaca cacacaccaa tccaagaagt aaaagaacgg tatgaatgta
    18661 attagaaaga aaattcttca cttgtataaa attcattgtc tgctttcgct ccttccgttg
    18721 tggtgaagta atccattgca aacacatagg cattgattaa tgcagctatt ggatcaattt
    18781 tgttactgtt ctttgattta ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt
    18841 tgttcactgc atgggtgaga atcgtatttt tattgtgaat aacgttgcct tcataaactt
    18901 gttctctaaa agttcgggtc ggaacgttta gagtaatcgt tccttgtctt acttctaaca
    18961 ttgggtagtt ggctttttca gctttactga taagcgaatt agcgttatac gggtcataac
    19021 aaatccccat acattccaaa tcattttctt gaatcaaatc ttgaataaac tgaaatactt
    19081 ggtcatagtc cacgattcca ctttctaatt gagtaatgga acactcacca gctttttcta
    19141 gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg aagcccatat ttggtggcta
    19201 caaagctatg tgaatcacaa taaagttcgc cgttatctag cggtacgatc catgaaacgc
    19261 tagttaaatc atctgtttta gataaatcca ctccgatata aacgggcttg cctgtaatat
    19321 cttgtggttc tacttcggtt gcctgccagt cttcagcaat catatagctg tcctcactcg
    19381 cttgtctcca taaattgaag tttttcacca atacagaatt aaggttattt tgtttcaaag
    19441 caaggttcac atcatcttga atggttggaa tcattacttt tttgatttct tcactttcaa
    19501 aaatcggatt ggctttgatc catttctctt gatcgtgaat ttcttcggga tcgtccaatt
    19561 cccatatcgc tataaagtac ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca
    19621 ataagtattc ctcgtacata ggaacgttta aatcaagccc agcggtacta ataacacaca
    19681 ataagccgtt cttttgttgg gtcattcctg acttaatgac gttgtacgtt ttacgagttt
    19741 ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc aaacccatct agcgtgctgg
    19801 tttcactagc cagtgccata gcgaacgaat tagagggcaa gtgctgaatc ttagagttca
    19861 taattttcag ttgtggtctt aaaaacttac tagacttgac cacgtttcta agtgaattag
    19921 acagcatatc atagcccaat ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc
    19981 ttgcttctgc tggttcttgt tccataatca acgcattggc agccatgcca cttgcgatat
    20041 acgttttccc attcttacgg ctcatactgg taaacgctcg attgaatcgc ctgtaattcc
    20101 cttctttagt acgccagcca taaagactgc ctagaatgaa tttttgaaat cctaacatct
    20161 tgattgcttt accgtccgta gatggtagca actcaatgaa tttaatcgct ttggtggctt
    20221 gttcttcatc gaacacataa gggaagctat ctgacttaga acgctccaaa tcgcttatat
    20281 gacgctcaca cgcttggata attttcttgg gtgcaatcag cttacctgat aggactttct
    20341 caatgtaatc attcatgaat tcatcaactc tttgaacggg tcttcgggtt catcattttt
    20401 aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc atacctagtg aggtggcttg
    20461 ttttttcaaa tcactcatag cttgtgattg aacccgaacc gctggattgg ctttctttac
    20521 tccgttttcg catgtgataa acgtacctga ttttcttatc tcggcttcgg ctgttttcat
    20581 tcgagcaaag gctaaacaat aggctaccaa tgaaccataa tctgcttcac tcaatggata
    20641 atcttttttt aatgttggta cgatacgatt ccattcactt ctagcgctcg ctgccatcca
    20701 gtcagggggc gtgatattta ttaatggttg ctgcttgaac aattcttctc tagcgtccac
    20761 acggattgct ttttcttcac ttgatatatt gccttttgta tcgtgtaata ttttagggtg
    20821 tcccatgttg tcctcctttc agacaaaaga aaaaggaacg taagcaatta agctcacatt
    20881 ccttcgtttt tcgatcagaa tttttcttta tctaatttat ctatataaat gatttttccg
    20941 ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct
    21001 gttttatcag taataacaat tgtatttttc aataaatctc ctactttcat tatatacttt
    21061 ttcacttgtg attacaagtg taatcgttat ttgtaattac ccttatgcct tttcacaacg
    21121 gttatttgta ccaaaatgag taggcatcga ttgtcagtaa gtttcacact atgccccctt
    21181 aactcaaagg gtactctttt ctcgtctttg tctttcattg gctgtcttgg aattatggtg
    21241 tgcatggcat aaagaacgaa aattagtttc atctaaccgt ctattccaat catcttttag
    21301 ttcaattttg tgatctacaa catcagcttt ccttatcaag ccttcttcta ggcaatcctc
    21361 acatactgga tgatttaacc gatacaagta tgaagccttt cgccatgttc tactttgata
    21421 gaattgaaaa tatttacctt cgctttgctt tctttcttca cgcttaattg ccgtttgctt
    21481 tgctttatgc ttcccacaat acttctgcct ataatcaatc aatgttttac atccagcatg
    21541 attacattgt tttttaactg ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc
    21601 atcttgttcc cattttgatt tgtattcaat ataaggcgaa actatcttca agcacgttcc
    21661 gtctttaaaa ttaactttag tgattttatc ttcctcaaaa ctaataaagt ctacatcttc
    21721 ttgagtgttt gaatactcaa tgtgtttctc accatcgaag tatctaatac tttccatatg
    21781 cataattatt ctacctccaa taaattagtt atcaattcat catctgttag tagcaaagcc
    21841 aagaacttat catggatgtt taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt
    21901 ctcgctcgat catataggtt aagcagacaa ttaaattcat ccattagctc acctgtatca
    21961 tcatagttgc cactatctac caatgatccc actactagcg attgacacca cgctactgac
    22021 tcctgcagcc aatcaggttc ttcttccatt atctttaata cttctggatc aaatgtggaa
    22081 taatcttctg ttaccaatcc cacactaaat gccagcgtgt tcattagaaa ccgatccatt
    22141 tcatctgtat attgaaagtt cggtgctttg tttgttgaaa taatattgag catttacttt
    22201 cctccttgat tcgtaacacg tggtaacacc tacgtaacag gtacccgtta ccactcaatc
    22261 ccaacgggga caagggttct agcgttttgg taacaggtaa cgggtaaaat ccagtaacat
    22321 tattccccag catattagct aactatttca aaagttttaa ggaaataagg tgttaccccg
    22381 ttaccaaatt gctgtatccc ttggggcagt aagggtaaac tagtaacggg ctaggtgtta
    22441 ctgacccgtt actacccgtt actttttgta ctttccagct cgatttttat tttcataggc
    22501 atttgactta ttgtaaaatc ctcgtcgttt atttcttttt ggcagttgct tccgttccca
    22561 accaggaata ctgctaataa tcaaggcaat ctttttagtt tctgcatctg atcgaccatt
    22621 caataaatcc tttgcttgtt tatcaaaaac agcttccaaa atatcagcgg tgagtacgct
    22681 atcaactaaa taaaactctt gttgcatagt agctccatta tagaaatgct gtaaattttt
    22741 ctcattacat ttactgtcag gatagttctg ataataagaa cgtctcgccc acgctggagc
    22801 ttcttcccat ttatacggga tttccatttc caaatattta gtgattgctt ctttaattgg
    22861 attctccatc ttggcatcct cttgatagtc ttttgcaatt tctaagactt ctttatcttc
    22921 atcgggacta aaatagattc gttgtccttt atcaaaaagc acctttgctt cagctaacac
    22981 ttgaagaaaa tattcatcct ttgttttaaa cggattcatc tttggcttat tcttacaagg
    23041 caaaggataa aaacggcggt ttcctgtatc atctttcaaa tactgcccat cattcgacgt
    23101 tccaataaat acacattgcc ttgcccaagc tatgacattc cgaccataag gaggtctaat
    23161 tttatcgatc ttggcactga tgaagttttt tatctgttca attttagttt tgttcattga
    23221 agataattct ccaagttcaa taattacatt gccttgaagc tgcatataat catctttctt
    23281 ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac
    23341 ggtactctta ccaatccctt gcggtccatc caaaactgga acgatctcaa actttattcc
    23401 tggtttatag atacgtgcga ctgatccgac tatccaacgt tttgttactt ctcgtgtgta
    23461 aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt tccagtctag gctgttgatc
    23521 ccatttaact ttttcgatac gttcttttac tgggttgaaa acattctttc tagccacatt
    23581 tactactgct ttgtgtattt tgttgtcatc aaaaactaca ttgtagtttt cttcaatata
    23641 tgccgttaag gcatcctcaa aactgtcttc ccattctcca gcggtacgat tgatccaagg
    23701 agaatctttt aataggtgaa tgtatccgct aaattcatta taagcaattg ttccagctaa
    23761 attgctatca ttggttaaaa tcaattctgc attcacaaca ctgttacttt taatctgatc
    23821 gtctttattg catcttaatc gtgacatcca ggttttcaat tcaatgatat tgtcttcacg
    23881 ctcgctggct tcgggaaagt ctaaaatact atctaattta tgcttatgag ccatgagctt
    23941 tccccctcaa atctttttta atcatcgatc gaataatttg attcacttct ttatctttta
    24001 gcggtggttc attgtacgca tttgctgtat aaatcaattc tgctaaagtt tgattatcta
    24061 caccgtgcca tagtaaccaa ccgcataact tagttaaatg atcgtttcta ctaccttcgc
    24121 cagcgccttg aacaatcata tctaattttt ttgctgtccc tgtttttgct acgggtcggt
    24181 attgagtctt tatttcttgc ggttttttta gcagtaggtt cactagccaa tcgggagctg
    24241 gtgcaatatt atttagagat acgttttcaa taggttcata tgctctccca acacttcttg
    24301 aaataatcat agttggcgct attggaactc ctttagtgat aatatcaatc ccgcttaaac
    24361 ttttaccttt atcatcacgc ccgaagatat cagcaaatgc acctgtttct tgttggataa
    24421 ttacaccatc aggaatttta aagaagaaat ggattccttt acctggtgtg atttccgtat
    24481 aagtagaagg aaatggatca tacattgaag caagtttttt ataattctca acgccgtttc
    24541 gtgtttcaga atgttgatct aggtcaacta ccaccatttg atgatcttca agcattaacc
    24601 caatatttct agttgcatct tccgtccacc attcattaat ttgattagaa tcgttagttg
    24661 catcattcca accactagag ccttttattg gaattttagt tccaggagat aagggataaa
    24721 ttgaccatcc cgacttttgt aattccgttg ctgcttgata attatttgcc aataaacctc
    24781 tcctcctttt tcaaaatact ggtataaatc attcagacac ctccatgagc ttcaactgct
    24841 caagttcgta tttcaaccca tcccgaacaa attcagcacc ttttaattga ttctgatact
    24901 ttacccatgt attaaatgtt ttcacccttg cttcttgcat atcagtaatg tttaaccatg
    24961 cggttaaaat ctgatttttt agctcgttga tttctcgaca aattattttg tgttctgcat
    25021 gttttttgag tacatacaaa actgttagtt tttcattgaa tgaatttcca aagtaggttt
    25081 tcccgatatt attttctagt tcagcgatcc cttgattaat ttcctctaat tcaaggctat
    25141 cgagttggtt taatagtttt gttttgtaca tctacttatg ccctccagtt cttaattgat
    25201 agtcgtactc gtcataagca gtgaagagat ataacgctaa tcctgcaaag atagctattt
    25261 ttgcaattaa tggtaataag ccgaaaattc cgccgattaa aatgcttcct ataattaagt
    25321 ttcgtttcat aatggttccc tccgattttt taatatggta taatcggaac aaagaatatc
    25381 gggttttatc ccaattaagt tgcctttatc ctgtccgcca agactgttat ggataaggct
    25441 ttttctttgt tctcgatcat gttaatgctc ctaagttgtc tgcaattcag cagaacgatt
    25501 ataaattttc tcactcactg aaaactgcaa cccatatttt tctttaacag tcattaattc
    25561 tactgtctca tctaaaatag gttcacgatc aaccaacatt tcaggagtca tatctttctt
    25621 ttttaccatt ttggaatgac cataaatagt tgatacagct ttattggcta tcacattcgc
    25681 tttaatgtag tctactggtt ttggttcagt aatagcgtgg cttaatttag tcattgcttc
    25741 tttttggtgt tctttgtcta gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg
    25801 gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat gcctcggctt cttttttacg
    25861 gcttttgaat gccatgcggt aaatattttt tttcgttgat gatgtttaca tcttgtgttc
    25921 tgcccaatga atcgatgacc ttactagtag taaccccatc tttaggcaaa gagtgaatgg
    25981 ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc tttcgccact gcccaccatt
    26041 catcattaat atcgacaaat cttataatgt gtccgttcca aatttcagtt ttcattcaac
    26101 gtcctccagt tcaagcccta gcaagctcgc taacaactca acttgctgat acaaaatttc
    26161 ttgatactct tcaatagaaa gtcgttcttt aaactctgct tgtactaaat cgttcaagcc
    26221 acgcaatact ccgcttaccg ttggttcttg tggtagcaat ttttgatagt ccatttgttc
    26281 gtacaaacat tcaaacgttt cttctagcgc tttgccttcc aagtttgcta attctgccgc
    26341 tgtgtcttct gtaagaattg ttgataatac tttttcgatt gctttatttt gtgtcgattc
    26401 cattattatt tcctcctatt aatccctttg cccccacggc ttgcgtttat ttttggtatt
    26461 cttccataaa gcgatcacat tcatcttgtg aaattcttac gactccatca actataatca
    26521 actttaaacc gttaggaaca tatttctgca tcatagtatt gtatgaaacg ttcatatact
    26581 tagcagcttg tgccttgttc atatattttg gaaactggta ctttttttct aataatgaaa
    26641 gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg atcgtcaata attttaaaag
    26701 ctggatcatc aagcgtgagt ttgtcaattt ttaattccat atttatcccc cctcgttagc
    26761 atacatttct atacaaagat tttaaaaaaa tgcaagaggc tcttgaatac atttctatac
    26821 ctcttataaa ctcaatatac catcatacct tttcatttgc aagaatttta acatactttt
    26881 ttgaactttt atgtttcaca catatttaaa tcgtgatata gtagagaaaa tatatgaatg
    26941 gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt ggagacagga caaagaatta
    27001 gactggctag aattgaaaag ggactctcaa tggaacaact tggaggtaaa ttatccccac
    27061 cggccagtaa aggtgccgtt agtaattggg aaaatggcta taatcttccc aataacacca
    27121 ggcttaaaca actttcaaag gtattagatg tatccactac ctatctatta aacggatctt
    27181 atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat
    27241 tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct aatgctatca
    27301 ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca aacgcaataa
    27361 aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca attgcttatc
    27421 tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat tcagaaaaaa
    27481 atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta aaagagttca
    27541 cagctcttgt tactgagcta aataaacact attcaaaata ataataaact gcctcctcca
    27601 accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca ttcaagcaat
    27661 atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg aacaaaatga
    27721 ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg
    27781 ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg aacaacgaaa
    27841 cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc cgagaagcca
    27901 ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat ggaaatcttc
    27961 gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg gctgaaacta
    28021 ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac gctatcaatc
    28081 ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga accaccacta
    28141 agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg tttgattacc
    28201 taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta cgctttctct
    28261 tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac attgattttc
    28321 aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc tataaaatca
    28381 gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga acaatttcta
    28441 tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt gtaactgatc
    28501 cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct atttatgcaa
    28561 gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat gtagcaagac
    28621 acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt caaactagat
    28681 tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca aaagaaacga
    28741 aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg gcatacaata
    28801 tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt tctcctagct
    28861 ttatagtcta ttcttcttct tcagccatga agttaagttc attgacgata attgaagata
    28921 gttgatattt caatgatttt tgaatagaac tactcatttt aaagatgata attcatcttg
    28981 attggttttc taatcgtatt caaaaacgta ttcaaattgc gtggcgtgct tttggtagaa
    29041 aagactgaag aatctatcaa aataaaatat gttaccaacc gatccttcca atgactcttt
    29101 tgggaatcaa agtaaaggaa taggctttat ataaagattt catttaacaa attgctatgg
    29161 actgacagtg tatattatca ggtactatca ttgagaacgt agccaatatc atatccaaaa
    29221 cagcaggtag agaagttatt tgaggaataa actgtttctt ttaataataa tttttaaata
    29281 gtaatgcaat ggaagaaaaa acattgggag atggatccaa tcaataagaa gattgtttgt
    29341 agaagtattt ctctcctttt aacaatatat tcatcctcca atagtctcaa ttgaattttc
    29401 ttgttttcat ctatgaagaa ttagtcatat aaatcgcaag aatacaataa tgtcgattca
    29461 catttctatc ataatagcta taaaagctat cttgttttac ttgatatttt ttcaataatc
    29521 aaaaaggaag gctgattagc cttccaagca taaaactaca ttgtgaacgg gatcttttga
    29581 aaaaaccgca ttacttgatt ctaattcttt gtagtatact ttagaatcat gattttcttg
    29641 ttttttctca tattcattat agtatactgc tttcattagt tttactaatt cttgattttt
    29701 tttatccata atcatcccat ctttctactt cacacgtgag ttagcgaaaa aaatatgaat
    29761 aacttttatt tgcttctgtt ctataccttc taatttttga ggatacagat tttctcactt
    29821 caaaattgga tttatactta actgctctct ttttattatg acatactgta gtcttttcat
    29881 caataatttc cttctgattc tttatttcaa tgtatccttt cgattttaga aactcattta
    29941 gttctgttgt ctcaataaat tccttaaaga ttggccatgt taagtattca tttactaaag
    30001 caacatcatc ctttcctgat tgagatgttt cagctattaa tttttctaaa atatcaatcg
    30061 ccactttgaa attatcatca attaaagcta aacacattaa atgctcattt ttagcaagcg
    30121 aaaaatcttc tgattttcta atttgttcca ttctttttaa atttcccgtc catttgaggc
    30181 tctgcaaata gttgacagtc aataaaagct tatttgatgc atctatttta gatgcatcct
    30241 tccaaagcat atcaaaaact gagtaagcta tctcatactt ctcgctcttc aagtagttaa
    30301 aagcaatatt gtggaatttt ttaaaaaaaa tattaaactg tgtaggaaaa aacttcttta
    30361 caacataaaa aactgacttt attgaaataa gaaaaataat attcatgttc tcttccaaat
    30421 attccaaaga tacacttatt tttttcccta caagaaaggc ttttttttca tcacttattt
    30481 tttttatact gtttagatat cttttattaa taacagcatt attatgaatg attaagtttc
    30541 ttcttaaaaa gaattctttg aatttctcct cgtcttcatc aactaactct ttaaaaaatt
    30601 ctttaaaagt attttttcca atatatttta gccatgtaat ttgcgaacct ctcataacat
    30661 cctcaacgga tttttcaatt aggtattctt ttgcttcttc taaactactt aattcaataa
    30721 tttgttcaaa agataattgc ttatcgttta tttttgaaga agatgaattg actatgataa
    30781 aagaaattag ttttgagtgt agtgattcaa aagcgttact tatcgtaatt aatgatgttt
    30841 caaataataa atgcctttgt ctagtccttt ttctactctc ttccaaataa atttcaatct
    30901 tacttctcat ttttgaatct tttagagata aagaaataga gtcatcttca cttaattcaa
    30961 atagaacccc tctttcttca ctagaaatca tttttagcaa ttttttgagc aattcgtctg
    31021 gagccccttc ttctggcttc ccaagctcat tgaatagtct tccgttactt ttttcgttaa
    31081 tattttttaa attgtcaata aaacgtgctt cattttcctt catttctata tacattagat
    31141 ttaaaagatt aaagctatca agaatacttt gttcttcttc atttaaaatt actttaagtt
    31201 gaaaagtcga acccataatt tcatcaattg aattaatata actgtcaact gcttccatct
    31261 gttcaataaa attccctaaa tcatctaata gaccttccat aaatatctcc ttatctctat
    31321 acaatttata agtataccct atcgttacat atcccattca ttattatacc agtatttctt
    31381 cgtgatacat aagggtgtaa cataataatt tcaaacgaag tgactacaag tagtcattga
    31441 agcaaatctt cataagacac ctgtagaata tctttgatgg ctttcaattg atccaactta
    31501 atgtgttggc ggccaccttc aatcttaacc aacgtttccc tagttatagc aattttacga
    31561 agctgtaggt ctctaatcag ctcggtttga ccaattcctt tttctaatcg gactcgtcta
    31621 atattgctac cgattacatt ttctttatca ataatttttt cctctttcaa gaatagcaca
    31681 ccttttggac taaaattagt cctttttctt attaatctta aagattcggt atgctacaat
    31741 gggactagta ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat
    31801 cattctaact tgcgctttac tcatcggaac tataagcggg tgtaccactt ctgacacaag
    31861 ttctacaagt acggaaacta gtacaacaac ctcagaaaag aattacccaa tacatttttt
    31921 tccggttcca gatggatttg aagcttatta tcatgataaa aaactagatg ttcctttatc
    31981 agaaactgaa tctactgaaa agattttgcc aattgaaatt gaaagaaatc aagacgatat
    32041 aaaactaaaa gctgatcttc ccttactgaa cagcacacaa attgatagac atgaaaatat
    32101 taaagttaat attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt
    32161 ttttgaggct ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt
    32221 taatgagacc caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat
    32281 gacgttgaaa aaaataaagt cgtatgatca tccattaaat ctaacattaa ataaagggat
    32341 actctctttt gtattagcag gttacgttga ctatgaatat acagcagatt atttggagac
    32401 gccccttcaa cacgataata acgatattaa ttttagattt atctacaatt caaaaagtaa
    32461 aacatggtta gttgatcaat tgagcactat aaatacggga tttcatgaat tacctaagcc
    32521 tgacaatgaa actgctttgg tcattaaaac attctaaaaa agaaacgttg aaggaataat
    32581 tattctttca acgtttcttt tattgttttt cagtttacgg tcttacctga gcgaataatc
    32641 tgatttctaa atgattggaa aacttcttgt ggttgataat aatctgtatc tatttcaaat
    32701 gtttctgaga ataattcatt gaatttttct actgaaatga tgattttacc attctcctct
    32761 cgactgtttt ctattatttc tgcctctaat tcaaataaat ctagtgacga actaaaatca
    32821 taaatcctgt catattgaac aaaaaaatca agaaaaagtt gctcaaactc ttccttattt
    32881 atcactagtg tttcaagcgg aacaacttta ttttcatcag ccattcttcc aatttcctca
    32941 ctttcatttt caattgtcca ttgttgcgga cgtatcagat tttctctatc atttttcagt
    33001 gtatagatca cttgtaatac acaacaatgc tttgtaaact tattttttta gttagtcgaa
    33061 tctttttaaa tgtgcgtttc ataccatgcg tttgatatat tttctcctat ttcttaaatc
    33121 gtttatcaca acttttatcg ttttttcttc tgaaaaaaat cttgtttaca tcgttacata
    33181 gtttccaaaa agatacatta ctagtagaaa caacaagatt aattttactt cccactgaaa
    33241 tgggaagtat attttaaata ttttttctcc
//
LOCUS       DACDLI010000026        31520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDLI010000026 DACDLI010000000
VERSION     DACDLI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.026"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..293)
                     /locus_tag="IV353_001259"
     CDS             complement(18..293)
                     /locus_tag="IV353_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009522.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            587..1402
                     /gene="thiD"
                     /locus_tag="IV353_001260"
     CDS             587..1402
                     /gene="thiD"
                     /locus_tag="IV353_001260"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9009523.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1607..3058)
                     /gene="cls"
                     /locus_tag="IV353_001261"
     CDS             complement(1607..3058)
                     /gene="cls"
                     /locus_tag="IV353_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9009524.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3400..6066
                     /locus_tag="IV353_001262"
     CDS             3400..6066
                     /locus_tag="IV353_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9009525.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6430..7182
                     /locus_tag="IV353_001263"
     CDS             6430..7182
                     /locus_tag="IV353_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9009526.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7204..8358)
                     /locus_tag="IV353_001264"
     CDS             complement(7204..8358)
                     /locus_tag="IV353_001264"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9009527.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8543..10984
                     /locus_tag="IV353_001265"
     CDS             8543..10984
                     /locus_tag="IV353_001265"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9009528.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11314..12018)
                     /locus_tag="IV353_001266"
     CDS             complement(11314..12018)
                     /locus_tag="IV353_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009529.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12205..12450)
                     /locus_tag="IV353_001267"
     CDS             complement(12205..12450)
                     /locus_tag="IV353_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009530.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12467..13318)
                     /locus_tag="IV353_001268"
     CDS             complement(12467..13318)
                     /locus_tag="IV353_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9009531.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13484..14488
                     /locus_tag="IV353_001269"
     CDS             13484..14488
                     /locus_tag="IV353_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9009532.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14729..15085)
                     /locus_tag="IV353_001270"
     CDS             complement(14729..15085)
                     /locus_tag="IV353_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9009533.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15453..16640
                     /gene="tuf"
                     /locus_tag="IV353_001271"
     CDS             15453..16640
                     /gene="tuf"
                     /locus_tag="IV353_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9009534.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16828..19419)
                     /gene="mprF"
                     /locus_tag="IV353_001272"
     CDS             complement(16828..19419)
                     /gene="mprF"
                     /locus_tag="IV353_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9009535.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19445..20164)
                     /locus_tag="IV353_001273"
     CDS             complement(19445..20164)
                     /locus_tag="IV353_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9009536.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            20373..21005
                     /locus_tag="IV353_001274"
     CDS             20373..21005
                     /locus_tag="IV353_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9009537.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            21064..21924
                     /locus_tag="IV353_001275"
     CDS             21064..21924
                     /locus_tag="IV353_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9009538.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            22031..24424
                     /locus_tag="IV353_001276"
     CDS             22031..24424
                     /locus_tag="IV353_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9009539.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24606..26447
                     /locus_tag="IV353_001277"
     CDS             24606..26447
                     /locus_tag="IV353_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9009540.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26471..27580
                     /gene="rpoD"
                     /locus_tag="IV353_001278"
     CDS             26471..27580
                     /gene="rpoD"
                     /locus_tag="IV353_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9009541.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            27800..29899
                     /locus_tag="IV353_001279"
     CDS             27800..29899
                     /locus_tag="IV353_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9009542.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            30095..30958
                     /locus_tag="IV353_001280"
     CDS             30095..30958
                     /locus_tag="IV353_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9009543.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            31063..>31520
                     /locus_tag="IV353_001281"
     CDS             31063..>31520
                     /locus_tag="IV353_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9009544.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT        10490 a         5531 c         6542 g         8957 t
ORIGIN      
        1 taaagaacag ttagaagtta aaattgctga ttcacataga gtttatgact tcttggaaat
       61 tctttttcaa tcagtttttt catctgttta tgaaattctt ttttttcagt aaacataaag
      121 gtttgggtat cgtaaccttc ttcatgtagg aaatagatta ttcctttttc tgtgttttgc
      181 gtataagctt ttatactcct gtcttttttg aaagaacaga tcgtatagcc atctatttgg
      241 tctggtagtc gtttgatttt cttcaaaatg acttttttat cctcaagttt catttttttc
      301 acctgacttt atttttttca tatggaaatc atagcgcctt tggttatatg aaaacaagaa
      361 ggatactgaa atatatataa atcaaatagt ggttatttat tattagaata gcgaatacaa
      421 acaaatgaca actattttct gatttattac aaatgttttt tcaaataaat ttgattacca
      481 agaaaaggta gtggtatatt tactgaggga tactttgaaa aacatatgat agaaacagta
      541 acagcattat gaaaaatctt tttataaaaa tggaggaatc gatttgatgg aagatcagta
      601 tgtacaagca atgacgatcg caggatcaga tagcgatgga agcgcaggga tgcaagcaga
      661 tatgcataca ttctttacaa gaaatgttta cggtgtttct gtcatgaccg catgtgttgc
      721 aggtaattct tacggaatcg gagcaagtgt gacattgcca acagatttta tcgacaaaga
      781 atttgaactg attgcgaagg actttcaaat acgtgcagct aaaacaggta tgctagcaga
      841 ttcaaagttg attgagacag ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt
      901 cgtggatccc gtaatcgtaa cgaaacatgg aaatctttta ctagaagaat ctgctttgca
      961 aagcttgaaa gaaaagcttg tccctttggc cgaagtactg acacctaatt tttatgaagc
     1021 agaaaagctg actggacgga cattagcagc tgaagaagat tttgtgcaag cagcaaaaga
     1081 actgcaagat atgggtgcaa aaaacgttat gatcaaagga catcatgcta aagatgcgca
     1141 gcaagaaaca gtcaatgact acgtattgct cgaatcagga aaatcatttt ggttaaatgc
     1201 accattttat cctaccgacc ggatcaacgg aactggcgat tcgctatctg catgtattgc
     1261 agctgagtta gcgaaaggaa aaacagttga acaagcgatc cgattggcaa aagactatgt
     1321 aaaccgtgca attcgtgaag aaataaatgt agggcacaaa tatggaccca tcaatcattg
     1381 ggcagctggt gaattagagt aaaagaaaga ggtcgtgagc ctgccgtcca gctccgagtc
     1441 acaaggaaca agttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
     1501 aggagttggc aggagaacac cgtttgttcg gaagaaggga ctgtgacaag atttttgtca
     1561 cagtcccttt aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag
     1621 aaaataatcg agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt
     1681 cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta
     1741 aaaactcagg atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat
     1801 cttgatttgc tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga
     1861 ggaagccgtt ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat
     1921 attgtgttgc tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat
     1981 ctacaccaga ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt
     2041 aaggtgtctg tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct
     2101 tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa
     2161 gcttagattc atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt
     2221 tttcttcagg taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag
     2281 cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta
     2341 catattgatt ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga
     2401 tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa tgccttttgt gaggtaacaa
     2461 atgtatggac atgtccacca tttttgatta gacgattgaa atcttttgct tttgtaccat
     2521 gagagccaaa tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta
     2581 aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga
     2641 tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg
     2701 tgagaagagt gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt
     2761 cgacaaattg ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc
     2821 ctctaaaaga ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt
     2881 tcttatccgt taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg
     2941 caaacaatac taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag
     3001 ccaccaaagc aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa
     3061 aaatgttcct ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc
     3121 aaggtgaata tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt
     3181 gctttttgta ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta
     3241 accggcaacg tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt
     3301 gtatgaaatg gtgagaccta agacaaatgc gtatttgtct taggtctctt tttttttcag
     3361 acaggactag ggccaagatc aaaaaatggg ggatgaaatt tggaaaacaa ggaacaagca
     3421 caacaagcgg tctataaaga aacgatcgac tcgttgatgc agcaaacgca gagctcaacc
     3481 gatggattga gcaccacgca agcgaaggaa aaattagaaa gagatggctt gaaccagcta
     3541 aaagaaaagc cgaagaagcc aacttggaaa ttgttcttag aaactttcaa agatgcaatg
     3601 gttatcgtat tgctgattgt cgcactgatt cagatggtca tgggtgacta catcgaattc
     3661 ttagttattt ttgcagtatt gatgttgaat tctataatca gtgttgtcca aacaaaaaaa
     3721 gcagaaggtt ctttagaagc gttgaaaaac ctttcagcgc ccgatgctcg agtgattcgt
     3781 gatggtaagg aaaaaacgat tccggcaaac gaacttgtgg taggagatat cgtgttgtta
     3841 gacgcaggcg attatgtacc tgctgatgga cgtttactag atgcgggatc attaaaagta
     3901 gatgaaggta tgctgacagg agagtcgact tctgcagaaa aagaagtaac ggatatcaca
     3961 agtacggtac caatcggaga tcgtacaaat atggtattca gtggaacgat cgtaacttac
     4021 ggacgcggta aattcttagt gacttcaact gggaataata cggagatcgg acaagttgct
     4081 ggccttttgg aatcaacaac tgagcaagtc actccgttac aacgaggtct ggataaattc
     4141 agtaaaaaac taagtctggc tattttggca ttgtctttat tgattttagg tatccaactg
     4201 tttcgtattt atcttggaga aggtacagga gatatgactg cagatatcgt cagcgcagtg
     4261 atgtttgccg tagccgtagc cgtagctgct attccagaag cattacagtc tatcgtaaca
     4321 atcgtgttat ctttgggaac gaataaaatg gcaaaacgtc atgccattat tcggaagctt
     4381 ccagcagttg aaacacttgg ttcgacaagt atcatctgta cagataagac tggtacattg
     4441 acacaaaaca aaatgacagt tgttgactac tttttaggta atggcgatac tggggatttc
     4501 acaaatgacc cttcaaaatg gacagctgac gaacgtcgct tgatcgaaat cagtgtttta
     4561 gcgaatgatg catcgatcag tgaagacggt actaaattgg gcgaccctac ggaagtagcg
     4621 tttatcgact ttagtgaaaa attaaaccag ccttatcagg aaatccgaaa caactaccca
     4681 agacaagcag aacttccttt cgattctgat cgaaaattaa tgtctactgg acacacgatc
     4741 gataacgaaa attacttgtt tgtcaaaggc ggaccagata tcgtcttcca acgcagtaaa
     4801 aaagtattgc taaatggtga agtcgttccg atgacggatg aattgatgaa aaaattccaa
     4861 gagcaaaatg aagctttttc aacacgcgca ttacgtgtac tagcttttgc ttaccgtcca
     4921 cttgataatt ttgatttatc atttgaagat gaagacgatt tgatccttgt tggtttgctt
     4981 gctatgatcg atcctccacg tgctgaagtg actcaggcgg taacagaagc aagaagtgca
     5041 ggaatcaaaa caatcatgat tactggtgac cacaaaacaa cagctgttgc tatcgcaaaa
     5101 caaatcggga tttttgaaga aggaaacctg gctttgaccg ggacggaact agatgaattg
     5161 acagaagatc aactaaacga agttttagaa aaagtcactg tttatgcacg tgtttcacct
     5221 gagaataaaa ttcgtatcgt tcgtgcttgg caaaataaag gtcatatctc agctatgaca
     5281 ggtgatggtg taaatgacgc accagcattg aaacaagcag acatcggaat tgctatggga
     5341 acgggtacag atgtggcaaa agatgcagca gcgatggtat tgacggatga taactttgct
     5401 tctattgtca gcgcagtaga agtaggacgt aatgtgtttg ataatatcaa gaaagctatt
     5461 tcttatctat ttgcaggaaa tttaggtgcg atcattgcga tcatctttgc cttagttgct
     5521 gactggtcca atccatttac agcattacaa ctgttgttca tcaatttagt caatgattca
     5581 ctgccagcaa ttgcattagg tacagaaaaa gctgaacctg ctacgatgaa ccgcccgcca
     5641 agagatccaa attcaggaat ctttacggac aaaacattga tttctgtaac gtatcgtggt
     5701 gtgctgattg ggattgctgt tatctttgct caatggcttg gcatgcagca ttcacctgaa
     5761 ttcggtgtag ccatgtcatt ttcaacattg atttggtgtc ggacattgca aacattccca
     5821 gcacgttcaa acacacaaac agctatccaa gcagggttct tctctaacag aagtgtctta
     5881 ctagctgtag ttgtatgtag cgcgctttac tgcttaacaa tgatcccagg actgaaagaa
     5941 gtcttctcta tcccatcagc atttggattg acagaagtct ttacatgtat gggaatcgca
     6001 cttgtgacaa ttgttttgat ggaaatcact aaattgatca ttgtacgagt acaaggaaat
     6061 aaataaaacg aatcaacgag gttgtgagcc tgccgtttaa ttctgagtca caaggtgcaa
     6121 tttcaacaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga agagttggca
     6181 ggagaacacc gtttaaggaa gaagttgtaa aatcagcatt ttgagctaat actgagggtc
     6241 atctgattgg acactgttta ttcggaaaga gggactttga cacgactttg gtcacagtcc
     6301 ctttttttat ttatgataga cggaaagcca atcattttat tattcatcaa aagagataga
     6361 atagataaaa gaggagaata atttattccg aaaagtgaaa acatataaca aaaaattaga
     6421 ggaaatcaaa tgaatgaaaa agaatgggat gaatttgctg aagaatatta tgaaatccag
     6481 caagaatcgc aagtaactat tgctgaagat gtaaaaaatt atctgaaaac ggagaaagtc
     6541 ctgcctgctg aaacagcagc agacgttgct ggaggatctg gcagatttct ttccttagca
     6601 aaagaaatta atcgctatga tttgatcgat ttttcttcag gaatgctaga aaatgcaaaa
     6661 aaagaagcag aaaaaaatga gttgccaaat gtttccttta tcaaacagtc gttagaagaa
     6721 tttatccaat caaatgagac atatgaaatg gtttttacag cagcaaatcc agcattggta
     6781 catcttattc aattagaaaa attgatgaaa aagtcaagca aatggtgcgt gataatcagg
     6841 gtagtggaat caatcgatga tgttttttat cctatcgaaa agcaactagg aatgatagaa
     6901 gaagatccta atacatcgcc agttgtaatg gatcaatttg aaacatactt aattgatgaa
     6961 ggattcaaag tgaagaaaaa agattttact tattttacac aggaatcaat tactgaagaa
     7021 ttgatgaagc tatactatga cgcgtacgca aaagatcctg ttgtgaccag gtacattcgc
     7081 aaactattca gtgaaaaacg agaaatcctt agtacgacta agctgaccta tagactgctc
     7141 ctttggtccg cagataaaaa aagaaccttt cacgactcgt gaagggttct ttttatgatt
     7201 ttattatatt ttgtagtaac gaatatcatt ttcgatcttt gtaatcgaaa aaggtacatc
     7261 tgaagtatat tctgcacatt ctccgtgttc aggaagcgtc tcactgaaaa tcgtttcata
     7321 ttcgtcaaca gtgatccgtg ttcgagaatc tagcatctct tgatgagatt gtgcgaagag
     7381 atattcttga tagttttctt ccagataacc tgtaaaaaat tcacttactg caccagaacc
     7441 gtagctgaat agaccgatgc gatctcctgg ctgtagtgat ttcgaatttt ccagtagaga
     7501 agttagcccc aggtataatg aaccagtgta aagattaccg attcgtcggc tgtaacggat
     7561 gctttcttca tagcgagcca taagacgttc ctgattttct tcgtctgttt ggtctaatac
     7621 gctttgcaat gccttttttc ccatcttagt atacggaata tggaaagcaa tcgcctgata
     7681 atcttcgagt cctcgacccg acaattcttt atgtcgattc caaacttttt ggaatgattc
     7741 gatatacgta gaattagata aaggaccatc aacaacagga aattcgctat aatctggacg
     7801 ccagaaatca tagatatctt ctgtcagaaa tacgctgtcg tcttcaatcg ataaaatacg
     7861 cgggttttga gtgatcatca tcgcaacagc accgacacct tgcgtcactt caccaccgct
     7921 tgccaagccg taacgagcaa tatcacttgc tatgactagt acttttcgtt ctggatgatt
     7981 tttgacatat tctttcgcca tgtgcaaagc tgcggtgcca ccgtagcaag cctcttttac
     8041 ttcaaaagaa cgagcaaaag attggatttt cagtaaatgg tggataatta ccgcactcgc
     8101 ttttgaatga tcaatcccgg attctgttcc gacgatgacc atgtcgatta gctcacggtc
     8161 tttttctgtt acgattttgc tggcagcatt tgctcctagt gtaatgatgt cttcatttgc
     8221 acgattgact gccatttggt cttgaccaat ccctatatgg tattttgcag gatcatcccc
     8281 acggctttct gccaactctg tcatatctaa atataaatta ggaataaaaa aggaaagacg
     8341 atcaatccct attttcattt caacacctct ttacttatga taaaaaactt actttactaa
     8401 ggtatcataa aaagattaaa agaaatataa aaaagagaat atttaaggat tgttaaaatt
     8461 taatcttaga aaaaaacaac tggaacaaga gctatttgtt tttacagcaa agaatggtat
     8521 atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc
     8581 gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt
     8641 gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt
     8701 cggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag
     8761 cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa
     8821 agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg
     8881 cggaacagag agtatgtctc aagcacctat gctgaaaccg tatcagtcag aaacaaatga
     8941 atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc
     9001 acatatggga ttaaccgcag agaaggttgc aacacaattt tctgtgagca gagaagaaca
     9061 ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt
     9121 ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc
     9181 agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga
     9241 aggaactgta acagcaggaa atgcttcgcc attgaatgac ggtgcctctg tggtgatcct
     9301 tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt
     9361 agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa
     9421 attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc
     9481 atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa
     9541 tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt
     9601 gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg
     9661 tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa
     9721 ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat
     9781 agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga
     9841 agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatgggcat
     9901 cgcgcagaat tttcaaatca atggtaagaa aaaatggatt ccgatggcaa cagaggaacc
     9961 atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac
    10021 acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt
    10081 gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc
    10141 ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag
    10201 tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa
    10261 catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga
    10321 agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg
    10381 tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa
    10441 aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa
    10501 agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt
    10561 tagcgcttcg attcacgcct acgcggcaag aaatggatta taccaaggat taacagactg
    10621 gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt
    10681 aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga
    10741 ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc
    10801 tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc
    10861 attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg
    10921 tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa
    10981 ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca
    11041 aatcttgctt ttttctctat cagaaaatat gtttattatt agaatttttt atagttaagg
    11101 ttaggcatct taagaaatca ggcaactcca agatatcttc aggtagaaga gaagtcgcct
    11161 aacattgtag gatcataggt gatttgttgg acaggtcaag ctgatatttg agcctgatgt
    11221 tagaaggaat ttctttttac ttgctgtaga aattattata tgactaaaaa ggctgggatt
    11281 ttaaatccca gccttttatg acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc
    11341 aaattgttcg atcaaaggaa tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt
    11401 ttcgataacg aactcatttt cagtagtcat agaaatagtt gctaatttac gaggttcatc
    11461 agcatttttt tcattaataa ctacttcatg ttcattttgt tctgtgtaaa atttaggata
    11521 ctcagcaacg gcttttgaaa aatcagcaaa acttaaagcg gcattcgtat cagttgagcg
    11581 tgtattttca gttgtgtccg cttttggttt ttccgttttt tcttctggca cactgactgt
    11641 ttcttctgta actggtgtcg caacagcttg cgcagttggt tcatcgtttt tcaatcgttg
    11701 atcattccag taagacacga tttcaataag aagattgtca cggaatcgtt cgaaaatccc
    11761 gttttctcct gaacgaattc tctttaatac agtattgaag ttgtcatcga ttggcacgtt
    11821 gatccgtgta tcttcatttg ttttcccagc taatccgatc gttaagattt gacggttttt
    11881 gggatcaagc atgacttgta acggcaaacc atagcgtcgc aagctctctt cttctagaga
    11941 acggaataat tgtcctctta caccatccaa tgaaggacgg aattgattta ataatttttg
    12001 tttctgtttt ttcttcatat gttctctcct tcgctcatac cctttcgatt ataagaaaaa
    12061 aacagccaaa tgaaaaggag taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa
    12121 aaatttgcgt attttcccgt tttttgaaag aaaaataaaa aatggaacgg attatgtata
    12181 tatacataat ccgttccatt ctttctattt tttcggtatc aaggcaaaaa caggtagttc
    12241 aggtgaataa atgacttcat gttcgtgcca gggtttgacc attagcgaat gatcgaaccg
    12301 ttttttcagt tcttcgatga tttgatcatg tgtccagttt cttgcaggaa aatgttggat
    12361 tttctctgca tcgatcaaaa agacaaaatc ggaatgaaaa gtctgggtaa cttcttccga
    12421 gagagaattc aagcgttcga ttggatccat aaaatcacct ttcgacttaa aattgggtag
    12481 tatctgggtc ttttgctttg ccgactacac catctaactg atcgatttgt ttcatttctt
    12541 cttctgttaa ttcaaaatca aataattcag tattttcttt gattctgctt ggtgtcactg
    12601 acttaggaag aggaaggaat tcgtgttgca gcgaccaacg caaagcaact tgtgcaatcg
    12661 tcttgtcatg tgcttcagcg atttgtttca tttcaggcac atcgaagatt ttaccagtgc
    12721 ctaatggact atatgcttcg aggatcatat catgtttttt tgcatactca actacagttg
    12781 gctgtaattc gcctggtgca aggaaaactt gattgaccat cggcataatt tttgctgttt
    12841 ttgctagtgt atctagatgg tggggtaaga aattgctcac accgattgct ttgattttac
    12901 ctgcttcata cagttcttca aatgctcgcc atgtatctgc atttgcttgt tcccaattat
    12961 cacgaaaagc aacagggttt ggccagtgga tcaaaaacag atctaaataa tctgtttgga
    13021 gcttcttcaa tgattcttca aaagaagaca tcacaagttc atagctgtgg tttgcattcc
    13081 ataattttgt tgtcaagaat atctcttctc taggaattcc actttcctta atcgcttggc
    13141 cgacactttc ttcattcttg tatccttggg cagtatcgat atggcgataa cctgcagcca
    13201 gcgcttcttt aacagaggaa acagcgacat ccccatcagg tgtttgccat gtgccgaatc
    13261 ctactaccgg gatttcgtaa ccatttaata gacgataagt gcttgttaat gaattcatac
    13321 tttcagctcc ttttttattc gttcaactat aagtgtagcg catttttcga aaaaaagaat
    13381 ccattttgtt tctactcttg aaatttttgt ttaaaagaaa atgattttga ttctggtatt
    13441 tttgattttt tttccgtaaa ctaagacaga agggaatgag aaaatggata tctatttaaa
    13501 aaaagcaatt ctgcatatta ttgacagaga aactggttct ccagtctttt cacaaaaaga
    13561 gctggatctg acaaaagaat atattcgtga ttttcttcaa aagaagatcc aaaaaatctc
    13621 ttctgctcaa acaaaaactg gacaactagc agaagaaagc atgatcgttg aatcacttaa
    13681 aatcgctcaa gaaaattttg tggaagcaag tgaagcaatc gttcagcggt ggttcgatat
    13741 ctaccaagaa agtgaagaag cacctagtgc agatgtattt gttgttttgt atgaattaga
    13801 tacagtgatg tacttatctt tattgaaagt caattatcgt gaggcttaca ctcactttgt
    13861 tgaagcagac gaagcaggga tcgacaaccg cttgattttg aatcgagcaa ttctaggggc
    13921 gaaaacacaa aaagcagatg aagcattcgc tgtgaatttg actgattgga cctacgaatt
    13981 aattgaaaaa aaatatgagt tttctggcaa aaaagagctt tatttttctg gaagagtgat
    14041 cgaaagtgtt cctgcacctt ctttagaaga aaatgtaaaa gtgatccaaa aagtagccaa
    14101 gcatcttgga aaaaaatttg agacagaaga atttgatatc atggctgatt tgaaagaagc
    14161 tgtctatgat acgatagaag aaaaaggtcg gttggatcat gagatgattg cggaaaaagt
    14221 cttcaaggaa aacatcacgg caaaaatggc ttttaaagaa gaagtacaag agcaaggatt
    14281 tgtcccagaa gcacctcctg taaaagaagt acaagaaatt tctgagaaaa aatttggaaa
    14341 acaaaaattc aagctatcaa acgggatcga actgatcgta ccagtagatg tatatcgcaa
    14401 cccagatttg attgaatttg tgaataatcc ggatggtacg atatccgtga tgatcaaaaa
    14461 tgtggatgaa gtattgaatc gtttataaaa atgactagaa cattaggtgt tcaagtacca
    14521 gtgatcagtt aaatgaattg tgtcaagaga aggatcggaa aagacgaata taacaaaaaa
    14581 agagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
    14641 ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt
    14701 ttatctaata aaattttaag accatttact aattatcgat tttttctgcc gcatttttag
    14761 gcacttcaga agcctttatt tcttcccagc ttaatacacc tttacgagga ttgactttga
    14821 gtttaagata cgcattcatc tttaatggat gttctctttc ttcgcgaagc gtcacctgtt
    14881 ttttttcacc atctttatta taagccgctt gcttgtaggt ataagtcgtg aatttctctc
    14941 cgttatctgc ttgggtagct gacatttcgc cattcgtcgt gatttttgta taataagctt
    15001 cgccgccgta aaaataatga taagctgcaa aacttccgcc gagaaatatg cctaaaacga
    15061 caagtacgcc aattaatttt ttcatgtact tcacctcttt ccttattatt aacgaaatag
    15121 aagatttcgt ctattgaaag ttgttggcaa cagctgaatt ttaaactttt cctcattttg
    15181 ttcttattgc ttcaaaagaa agttaaggac tatcttgaag tttttctcaa aaaacacatt
    15241 tttttattta taaaaggtac ttttttcgcg tataagacaa gcgagtgctc ttagttgact
    15301 ttgaccaata ttgacgtata ataagcttag ttcgaatctt acaaaaagta agtcggacgt
    15361 attctttttg atttagtcaa aaaaagacta taataaaaag gtgagagctg aaatttttca
    15421 gtgatctata ttatttagga ggtttgtaaa ttatggcaaa agaacattat gacagaagta
    15481 aaccacacgt taacattggt acgattggac acgttgacca tgggaaaact actttaactg
    15541 ctgcgatcac tacagtatta ggcaaaaaag gcctagcaaa tcctcaagac tatgctagta
    15601 ttgatgcagc tccagaagaa cgtgaacgtg ggattacgat caataccgca cacgtagaat
    15661 atgaaacaga aaaacgccac tacgcgcata ttgatgcacc aggacacgcg gactatgtga
    15721 aaaacatgat tacaggggca gcacaaatgg atggtgcaat cttagttgtt tctgcaactg
    15781 acggtccgat gcctcaaaca cgtgaacaca ttttattgtc acgccaagtt ggtgtaaaat
    15841 acctgattgt tttcttgaac aaagttgatt tagtcgatga tgaagaattg atcgatttgg
    15901 tagaaatgga agttcgcgag ttattgagcg aatatggttt cccaggcgat gacactcctg
    15961 tgatcaaagg ttccgcatta aaagcattgc aaggcgatcc agatgctgaa gctgctatta
    16021 tggaattgat ggatacagta gatgaatata tcccaacacc agaacgtgat acagataaac
    16081 cattactatt gccagtggaa gacgtcttct caattacagg tcgaggaact gttgcctcag
    16141 gtcgtattga tcgtggtgct gttcgtgtcg gtgatgaggt agagatcgta gggatcaaac
    16201 ctgaaacaca aaaagcagtt gtaacaggtg tagaaatgtt ccgtaaaacg ttagattacg
    16261 gggaagctgg ggataacgta ggcgtgttgt tacgggggat ccaacgtgac gatatcgaac
    16321 gtggacaagt gcttgctaaa ccaggttcca ttactccaca tacaaaattc aaagcagaag
    16381 tgtacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcaac aactatcgtc
    16441 cacaattcta cttccgcaca actgatgtta caggaacaat cacattgcca gaagatacag
    16501 aaatggtcat gcctggtgac aacgtgacga ttgatgttga cttgatccat ccaatcgctg
    16561 ttgaaaacgg aacgacattc tcaattcgtg aaggcggacg gacagtgggt tcaggtatcg
    16621 ttacagagat cgaagcataa gaaacgcgac tcgaatattt tggtaatgaa aaatgtttaa
    16681 aataaaagag cggtccaaac aggatttctg tttggaccgc tctttgttct tcaattgtat
    16741 aagaagagat tgtagaagca tgaactttcg ttttacttca gtctctttta ttcatcatga
    16801 gagtgttcat ctatcggtat gcagttttta atactttctt cgtatccatt ttttcaaacc
    16861 acttattcta ccttgtgtct gctcaactgg cgcattatca ataatcagca aagcaatcat
    16921 aacatatgca atccaactat ctctagaata tagagtataa cgtgagaccc agtttgtagc
    16981 gtacttgctt ttatattctc ttaatccatg gaaagaataa aaatgagaac caaactcgta
    17041 aaccaagtat gcaatccttt cctgtaaaaa actctttctg gaagttccga catttgaaag
    17101 aggggccatc cctaaattga accattggat atgttcttct ttcatatagg caaacaaatg
    17161 caagaacaag aaatccatac tgccagaagg tgctttttta ggatcatatc gcatcagatc
    17221 aatcgttcct actttattgt tcgtgtaagt aggcatgatc gttgcaaaag cgacaatttc
    17281 ttcttgggta tttttgacaa ctgcaatagg cgctctttgt agataatctt cagagaaaaa
    17341 gcctaaagaa aacccttttt cctttcgtga acccaaccaa ttatcagaga tatttttgaa
    17401 agtactcatt tgctcagtag aaaatggtgg ttgtaagaca tcaaatgtaa atccttctcg
    17461 ttcgatccga ttcaagacag cacgagtgcc cttcattttt ttccctgaag ttgtaaaact
    17521 attcaggtct acataagctt cttcccccat cttaatgaaa tcgtagccga attcatgcaa
    17581 aatcataaca atctcttcgc ttgtttcgta gaaaactggt aaatagcata gtcgatctgt
    17641 ttcttcgata aaagcttcga ttgcttctgg aaaatcttct ttcttaccag aaggatcacc
    17701 cattacaata catttattat tgaagcaagc gaattggaga agaacagttg gttcaccgtc
    17761 tttttcatag gcaaacatcc gcttatcttt taagaagatc aattgactat cggagtttcc
    17821 tccataagtt gtaaggatct tcagggcctt ttcttcatta aaggcttcgc caatctgttt
    17881 cttttctcct tgaaggaaat gaacaaatag aacgatcatg aaactaacag cgatgatggc
    17941 caacaatccc gaaaaccaaa tcttttcaga aggaaataag aaaaaggaaa taaaatggtg
    18001 acgccgatga gggaaatccg gcagattgta taccccaata acgatgtaaa gcaaggacag
    18061 tgcaccaata atgattccat cgatcgtacg ccactcccat gaatagatca attgttcccg
    18121 aaataattcg tttttagaag caagaatgat cagaagcaga atagacaaga aaaccaccgg
    18181 tgtgaaacta aaatcactta ataaaacata aagtaaagct agaacaatca agaagattgt
    18241 aggcaaatat gctcgtttca cacgtgctgc gatccctctg cccataatga ttaaaagaaa
    18301 tcccaatagt atactaggaa attggataat aaaatgaaat ttcaatgggt tcaaactatg
    18361 aagccagcga aactctgtaa aagcttgcgg gatagtggca gaaagtacta gcatgattcc
    18421 agaaaagtat aataagacaa ccacgatttt atgcgcaatt tctgttgcta attgtttagg
    18481 gataccagaa taacgctgat caaaagtgga accgatattt ttgaagaaaa agacgattcc
    18541 aatcaaaaac ggaatgatgt agtagaacag gcgatagagc aagatccaca caacgactat
    18601 ttctcttggc acaccaatag cggacaaccc gataatcatc attacatcga aactcccaat
    18661 ttctcctgga atcatagaca cgattcctat cactgaagca gcaatgaata acggaattgt
    18721 ttgccacaga tcaatccgga tttccattaa ataaccaaca gaaaggaaac taaacaggac
    18781 accggtccat tccaacaatg aaactagcaa tagctgtcct tgagttttaa gtgaggtacc
    18841 accaaggaat cctttctttt tgaaagtagt aaataaaaag acgacaggaa aatatagacc
    18901 gccgccgatc aaccatatcc aatattgctt caaaaaagga gcaactggcg tgaagactac
    18961 aagtaaaaaa gaaagcagac tgtaaataga aagtccagcc atcaaaaaca agaaaacttt
    19021 cgataatgct tggatcacgt ttctgctctc tttcttttgt ccatatagtt cagagcgaag
    19081 ccccacacta acaaacccgc cgaaaccggc aatattgttg attgtattga tcagccagct
    19141 tgtctcaaac aagtaacgag gattctgctt ttgttcaagc atccgattca aaataatatc
    19201 ataaccaatc ataggtaaca cagagacaag accaatcaac agcataagtc cgcttttcca
    19261 tacaggtata gtggcaaacg tttctcctaa ttgctggata gatagagttt taccgatgga
    19321 catcaattca ccgacaataa tgacaatgac agaaatcaaa aagattgttt tgaacagtcc
    19381 taggtggttt ttcagccatt gtatggtatt ttttaacaaa taaatcacct tctattccta
    19441 atagttatgt ttcagaaaga aattgaagaa cttctcgatt gaagcgttca ggatcttttc
    19501 tagctaattc atgtccttgt tctttcacaa gcacaaagga agcttttggt atagttttag
    19561 ctatatacaa agaatgtttt agcttgatta catctttttt tcccacgatg atcaaagtgg
    19621 gggagttgat tttttttaaa tcattttctg taagcccgat gtcatgcagc aaaagtttga
    19681 taactaaaag atttttgcga agacttgggc gaaataaaga gagtaaccat acccatgcat
    19741 aatgaatatt actgatgact ctggcagaaa aacgaacgcc tttaacaagc gtgtttccag
    19801 aatttaaaat caatcgatga acttttttag ggaagctgct ggcaaatacc agtgctagat
    19861 ttgctccatc actaaatcca agaaaatctg cctgatcgat tttttccagc aacatgatcg
    19921 tattcagatc ctcggccatt aactgaaaat tcaacataga tgcttcgttt gtcgaacgac
    19981 catgtcctcg gctatctact agatacacgg tatagtagcg ttcgagtaca gggacctgtt
    20041 ctgaaaaaaa gcggccgctg ccgtcattgc catgcagtaa aaagagaggg aagccttgac
    20101 cgctcttttc atagtaaatt tcgctcccat ctggcatttt tgcatatttc ttttcttttt
    20161 tcacataatc acctactgtg taaagtataa cgattatatc agtcattggg caattgtttt
    20221 tactgaaaaa caacgatcat tttcgtaaga tgacaaaaaa tacaggaaaa ttcatttttt
    20281 cttaaggcgt acacagaaat caaggaagaa atagccaaga agataaagaa atagtaagat
    20341 aaaaaaagat gtcaaaataa ctaggtgatg aaatgaagcg aaaacgatta tctattatcc
    20401 gtattctttt ttacatgatc attctgacgg ttattggtgc ggtgttgttt ttaggaggaa
    20461 gaacttatcg ttcggtaaaa cgagtggaaa gctatcaaat agaaataaca acagctgcaa
    20521 aaaattatca tatggaaaac tacgagaatt tgatcaaggc gataatattg accgaatcaa
    20581 aaggaaaagg gacagatctc atgcagagct cggaaagtct gcatggagaa gctgagatca
    20641 ttgataatcc agctgacagt attgatcaag gtgtcaaata tttggcggaa atgatagtag
    20701 aagcaaagaa acaaggatgc gatttatgga cagctgtcca agcatataat tttgggaaag
    20761 attatattgc ctatgtaaaa aatcatggcg gagaaaacac agtagaatta gcagaagcgt
    20821 attctaaaga tgtgctgagc ccacttttag gaaatgagga agggaaacaa taccgatatt
    20881 ggcggatcca gtccttgctt tacaatggcg gctatctcta tcataatgga ggtaacatgt
    20941 tttatcctga tattgtcaaa atgaatgaaa aaataataga gacctatgaa aaagttttta
    21001 agtaaaatag caggaaatgt tcaatcatga ataaaattaa atgaaagaag gagtgcgcaa
    21061 atagtggaaa aaaggcaaaa aatggaaaat caaaaagtgg ggtacacaac caaagactgg
    21121 atgttacggt ttgtgaaggg aatgtttatc ggttcaggat ttatcttgcc aggagtcagc
    21181 ggtggtgcat tagcagctgt ctttggcatg tatgagcggc tgatttcttt tttagcccac
    21241 cttacaaaaa atttcaaaga aaatgttttg ttttttctgc ctgttggtct aggcggagtg
    21301 acaggcatct ttctactttc ttttttagtc agttttttat taggtgcgta tgaaacaacg
    21361 atattatggt tttttgtcgg atgtatcgct ggaactgttc ctgcgttatg gaaagaagca
    21421 gggaaaaaag gaagagatac ttctgatgtg atcatcatgg ttgttacttt tgttctggct
    21481 tatttgtttt tattatacgg cgctcaatta tttgacgggc aagtcgatca gaattttgga
    21541 acatggctga ttgccggcgg attgattggt ttgggtatga tcgtcccagg attgagccct
    21601 tctaattttt tggtgtatat gggattgtat aaagcgatgg ctgacggatt taaagaagta
    21661 cgacttgaag tgattgtgcc gatcggaatt ggtgggattc tttgtgttct tggattgtca
    21721 aaaatcatgg attacatttt ctcaaaggca tatagcaagc tattccattt cattttaggt
    21781 gtagtttttg cttcaacgat tatgatcatt cctaccgatt acgcgggatt gggagttgct
    21841 ggggtactag cttgtcctgt tctttttata gccggtacag cactaggttg gtggatgagc
    21901 cgtttggagg aacaatataa gtaaagatag attgataata aaaacagcac acgggtgatt
    21961 caaacaaaaa ggttggcatc ttttttgtaa aatcattcgt gtgtttttct ataaatttta
    22021 taaaaacaag ttgtttttaa tcttggaaca cgctagactt aaacacgtga ttatttttga
    22081 aagttggcgg aaaatggaga aaaagaaaat gaatggactt acacagcaag aggtagttca
    22141 gaaaatggct tctggccagc aaaatgacta ccaggaagat gcatcaaaaa gtacaaaaca
    22201 aatattcagc gataatatat tgacgttatt caattttctg aactttggta ttgggatttg
    22261 tctgttattt gtaggtgcgt attccaacat ggcgtattta gcaattattg tcgtcaatat
    22321 cgttattgga atttatcaag aaatacatgc aagaaatctt gtacggaaat tatcgattgt
    22381 ttcccaatca gaagtactag tgatccggga cggtaaagaa caatcgattt ctactaaaga
    22441 gctggtatta ggagatttgg taaaaatcgg agcaggagaa cagataccag ccgatttaca
    22501 ggttatatct ggacgggcag aagcaaatga agctcttttg acaggcgaat ctgatttgat
    22561 tgtcaaagaa actggagctg aattgctttc tggaagcttt ttgacaagcg gttctgtcat
    22621 ggctgaagtt atccgagtag gtgcagataa ttatgctgtc cgcttgacta gcgaggcaaa
    22681 gactcacaaa gctattcgtt ctgaacttgt agattcgatt cgcaaagtat ccaaattcac
    22741 tagtttcgtg attattcctt tggggatcat actatttttg gaagcactgt tcatccgcga
    22801 tgcttctatc aaaatttctg ttattgcatc tgcagcagct ttgttaggca tgcttccaaa
    22861 gggtttggta ttgctaataa gtattgcatt aacgacaggg gtaaccaaac tggcaaaaaa
    22921 acgaatcctt gttcaagaca tgtattcaat tgaaacgctg gcccatgtcg atacattatg
    22981 cttggataaa acagggacca ttactgaagg aaaaatgagt atccagcggg tagaagcact
    23041 acatccgctg tacgagaaag ccatcccaga aattatgggg acgtatctgg cagaaagcag
    23101 tgataacaac atcactatgc gggctatgag agattatttt gagctttcta atcggtatga
    23161 tgtgacaaac aaagtcgcat tttcttctga tcgcaaatgg ggcgcgatgg aatttccaga
    23221 actgggagtc gtgtacgtag gagcgcctga acgtttggtg gaagaagatc ggcttccgga
    23281 cagtctaaaa acagcccaag cgaatggcta tcgcgtactt atgttaggga tcgctccaaa
    23341 tcagcaattg aacgaagaaa aaccagcatt tgttgagccg gttgctctgt ttgaaatcga
    23401 tgatccaatc cgaaaaaacg ctcaagaaac actagcttat cttcatgagg aaggtgtgga
    23461 tctgaaagtg atttctggag acaatccttt tactgtctcc aatatcgcta gacgtgcagg
    23521 attaccgaat tatgatgcgt atgtcgatct ttctcaaatt actgaagaaa tggaagtgcg
    23581 agaagctgct catcgctata cggtttttgg acgagtgagt cctcagcaaa agaaattgct
    23641 ggtaaatgaa ttgaaagaaa gcggcagaac ggttgcaatg acaggcgacg gtgtgaatga
    23701 tgttttggcg ttaagagaag cagattgtag cattgctatg gcagaaggtg atggagcaac
    23761 acgtcagatt gcgaatctgg tattgttgga ctctgatttt actacattgc cagaagtcct
    23821 gtttgaagga cgccgagtgg tcaacaatgt gacgaaagtc tcaggcatct tttttatcaa
    23881 aacgatttat tcatttattt tatcgattat atgtgccgtt acagcaattg gatttccgtt
    23941 tatccctatc caaatcacgc tgattgattt agcgattgaa gggtatcctt ccttcttttt
    24001 atcttttgaa caagataaaa gaaaaatcac ttaccgttat ttgcctacag cacttattaa
    24061 tgctttacca aatgccttgc ttgtagtagt gaatatcatt gcggtgtatc tacttggaaa
    24121 aacattcgca tggggaaatg ctgaaactac tactttgatg tattacttgc tgattgggat
    24181 cagttgcatg gcagttgtaa aagcttgtta tcccttcaac ccattgcgca tcttcctagc
    24241 agtaacgacg atcgtaggga tatatgtagc tgcaatgctg ttccatggaa ttttggaagt
    24301 aaacttaggg ttgggagaga gcgtaggata cttctttgta ttcatgctcg taaatattgt
    24361 tcttcgagta ttgtactgga aggctgatat tcaaaatcag ttactgaaaa gagtagcaaa
    24421 ttgatgctta atgagataga aatgtgttat acttgaaaaa gcaatgaaat atgatacaat
    24481 caaacatgta taattgtgtc ctttaaaatc gcactgattt cagttagtgc gattttttac
    24541 cggttattct cctgaaatat cttctgttaa acccaaaaga tatcaataga gcagggggtg
    24601 gaaaaatggc tcaacggatt cctcaagagg ttatcgaaac agtacgcagt caaacgaata
    24661 tcgttgaggt gattggacaa tatgtccagt tgaaaaagtc tggtaaaaat tacttggggc
    24721 tgtgtccttt tcatgaagaa cgtacaccgt ctttttctgt tgcagaagac aagcagattt
    24781 ttcattgctt tggttgtggt aagggcggaa acgtgtttac ttttcttcaa gaactagaag
    24841 gtctaagttt tccagaagca gtggtaaaag tggcagactt cgaacagata ccgttagaag
    24901 atcaatggcg tcagcgtact atagctgtcc aaaatcctga atctgctacg gggaaactga
    24961 ttgctgctca tgaaaaagca gcggaaatct atcatcacat gctattgcat acaaaagctg
    25021 gacagccagc tttagaatat ttgcaaagtc gtgggttgtc tttagaatta attgaagaat
    25081 tcaacatagg ttttgcacct aatgagcgtt cttttcttca gcaagtattc aagaatgaat
    25141 caatttcaaa tgaattgtta gaacgctctg gattgtttgt cacgcatgat gatggccgta
    25201 tgtctgatcg tttttatcaa agagtgatgt ttccacttcg taattctcaa ggccagacga
    25261 tcggtttttc aggacgctgg cttgcacctg aaaaagggca ggaaaaagat cagcccaaat
    25321 acttgaatag tccagaaaca gaactgttca ataaaagaca agttttattt aatcttgata
    25381 aagcaagaag cgatatccgg aaaaacggag aaattttgtt gttcgaaggg tttatggatg
    25441 tgattgctgc atggcaggct ggtatcaaaa ctggattagc ttctatgggg acaagtctaa
    25501 cagctcaaca gatccaacaa atggaaaagc tgacgaaaga attggtcttt tgctatgacg
    25561 gagacaacgc aggttttgaa gcaaccaatc gagggattga attgcttcgg caaaacagtc
    25621 gcttagcact ttctgtcgta gtcgttccag aaaagcttga tcctgatgaa tatctccgta
    25681 agtatggaga agcatctttc caagaattag tggctcatgg gcgcgaaaca gtctttactt
    25741 ttaaatcacg ttatttaaaa caagggaaaa atttagataa tgaaaaagac aagatcgaat
    25801 acctcgaaga attactagtt gaacttagcc gagttgtttc accaatcgaa caagatatgt
    25861 atctatctca actgtcaaca gagtttcaga tcggcagaga gacgatacaa aaacaactgc
    25921 gcgatcttcg aaggacaaat cagcaaaatc gtcaagaacc tcaggaaagt caacacactc
    25981 aggttacgag acaagaaact gcacggaaaa agagaccttt gacgcaagta gaaaaggcag
    26041 agcagattct attatatcgt gcatttaaag aagtttctgt ccgtaatatt ttaaaagaac
    26101 gagaatttca gtttattcat gatgcatatg cagaagttta ttttttattc gatgcatatg
    26161 tctcacaaca ttatgaattc aaccttgcag agtttttaga ctttttgcaa gatgaaaatt
    26221 taaggcggtt ggtggtggaa atcgaatatt taccggtggc agaagaaagt tcgccacgag
    26281 aaatccaaga cttgctagat gtgatcagca aatctggctt agctgatgaa ataacattaa
    26341 aaaaacaaat gcagcaagaa gccagacgaa ctggaaacaa acagcttgag cttgaattga
    26401 cgattgaaat catcaattta acgaaacagt taaaacaagc caaatagcta ctcaaggggg
    26461 gccttctttt atggcaaatg gaacagataa aaaaacgtat gaagcagcag tagcagcttt
    26521 catcaaagaa aacaaaccca aacgacaagt tgtatatgat gatttatcca atcagcttgc
    26581 tacaccattc actttgaatg cagatgaaat ggacaaattg atccaaaaga ttgaagacgc
    26641 aggtatcagt gtggtggatg aaaatggtga tccatcaatt cacagcttga agagtgctga
    26701 gaaaaaagct gaacaagcaa aaacagaaga tctttctgcg ccaaccggtg taaaaatcaa
    26761 tgacccggtt cgtatgtatc tgaaagaaat tggccgtgtc tcccttttaa ctgcggaaga
    26821 agaagtagcg ttagcattga aaatcgaaga aggcgaccaa gaagcgaaac agcgtctggc
    26881 agaagcaaac cttcgtttag tagtaagtat cgctaaacgt tatgtaggac gaggcatgca
    26941 gttccttgac ttgatccaag aaggaaacat gggactgatg aaggcagttg aaaaatttga
    27001 ctaccgcaaa ggatttaagt tttcaaccta tgctacatgg tggattcgtc aagccatcac
    27061 tcgagcaatc gctgaccaag cacgtacgat ccgtattcca gttcatatgg ttgaaacaat
    27121 taataaattg atacgtatcc aacgtcaatt gctgcaagac cttggaagag aacctacgcc
    27181 agaagaaatc ggtgcggaaa tggatcttcc aacagaaaaa gtccgagaaa tcttgaaaat
    27241 cgcgcaagaa cctgtttccc ttgaaacgcc aatcggtgaa gaagatgact ctcacttagg
    27301 tgatttcata gaagaccagg aagcaacaag tccagcagaa cacgcagctt atgagttact
    27361 aaaagaacaa ttggaagatg tactagatac attgactgat cgggaagaga atgtccttcg
    27421 cttacgtttt ggtttagatg atggccggac gagaacattg gaagaagtag ggaaagtatt
    27481 tggtgttact cgggaacgta ttcgtcagat cgaggctaaa gcattaagaa aattgcgcca
    27541 tccttctcgt tcaaaacaat taaaagattt cttagaataa gcgtggacta ttgaccagaa
    27601 gagaaatcag taaaactgtt tggtttattt taaaagaaaa aaagcttgag actacgcagt
    27661 ctcaagcttt tttttctata ggaaggaaat tcattcatta tttttaagta aatcctaaaa
    27721 ataagctatt ggcgtgacgt atagaatatg atagattaaa tggtagcgta aacctagtaa
    27781 aggagccgat cgctaagtga tgaaaaaatg tccaaattgc ggatcgaaaa aaatggagca
    27841 gaatactatc tgtccagact gcggtcatca aataactgca gaagatcagc aaataaaaga
    27901 atcaattgaa gaaacaaata cagatgcagc tatgcaggat aaatcaactg aagatgcagt
    27961 ctttcctgat acagaattaa acgatccaat cgaatggtcg gaattgaaag atttacctct
    28021 agaatctgta atggaattat tcgaaaaagc agaaccagaa gacgaagaac cacctgctca
    28081 cgatcaagta actaatatag agaaaaatga acagaagaaa gcaactgttc atgagacttc
    28141 ggaagaatca ggaaagaatc aagaagaaac gcttgaatca aaacaaaaaa gaagagtagc
    28201 tgaactgaaa gaaatcgtcg ataatgagga agaaaattcg atactatccg cttatatcaa
    28261 agcacatcgt gaagatacaa aagaagagca cgcgaaagag ctgctcaaaa tgatcagtga
    28321 aaaaattgca caggaaaaca aagtagagcc agaaaaggaa caagaaactg aaaaagactc
    28381 tgatagagaa aacgccacag gtaaagaaac agtaaaaata aaagacgtaa aaacggatat
    28441 cacagaaaaa actgaagtat ttactgaaaa acaattagaa aaggaaaaaa cgaataaact
    28501 gccggaagca gagaaaaaag aaatactcga aaacacagac aaaaagtcaa cagagcccga
    28561 tgaaataaat agtgaatccg atgctgaaaa tcaatctgta agcaaagagg gtactgatct
    28621 tgaagcaaaa atagaaaaag aagagaacca gcctaagctg tttgaagcag aagcgaaatc
    28681 aataacaatc gtgccagata ctgaaaaaac acatcaagaa tcaaacttag aaaaaacaga
    28741 tccagataaa gcgcaaaaga aaaaatcaaa aaaagtacct tatctcgttt tagcagcagt
    28801 acttctactc ggtggaggaa gctgggctta ttatgaccat catcaaaaag tagaagccga
    28861 agtagcagca gaagcaaaaa gacagcaaca aatggaagaa cttcatgaga aacttgctgc
    28921 cttttataca gatgatcacc atcaatttat tcgtgcagat atgattcatc aagatttatc
    28981 aaaactaaaa aatgatttat ccagtcttaa gaatgaaaaa ggatattcag agttagaaac
    29041 aacttaccaa gatatccaat ctaagatccg agatatccaa aaagtgaatg agtggttcat
    29101 agcacctgcc atcgtcgatg atcatctagc agatgacatc aagttgaaag cagataaaga
    29161 gatccaagca atcgaaacgg ataacacaga ttttggaaaa ttgattcaac aagcacaaac
    29221 agaagctaaa aatcaatatg ctgaattgca aacagctaaa gaaaaaacag ctatagtctt
    29281 taaagacgga aaagtagtgg attcagcaac acgcgaccaa tacaagacgg caaaagaagc
    29341 agtagaaaaa gtgaaaaata cagatctatc tgctcagttg aatgaacaat tgaaacaagt
    29401 agataagatg ctcactgata aagagaaaaa agaagaagag gaaaagaaaa aagcagaaga
    29461 agcgaaaaaa gcagctgaag aacaagcaaa acaagccgct caagcagcag ctgcagctca
    29521 agccgctcaa caagctgcgg tcgcacaagc agcacagcaa gaagcggcat cctcgacccc
    29581 ttcctcttca aactcagcga atcagccaat caaatctgcc agagcaagtg atattgccga
    29641 tgcatctaat ccagcatgga cgtgggcacc aggtgtccaa agcaaagtga ttgctacatg
    29701 tatccaacgt ggatatatcg ttgaaggagg ttacagatta gaaaaagcca gaatcgaaaa
    29761 tggggaaggc tattataatc tatatgccac ttcaacaaaa tctgcattga tgaaaggcat
    29821 tggagaaagt gctttgccat tttacatcgt aaccatcaac tgtaaaactg gatggttcgg
    29881 aggaaatggg agcaattaat tttaggcaaa tgaaagaggc tgtgagcctg ccgtttaaca
    29941 ccgtttattc gaaaaagggg agtatgacac aactgttgtc atactccttt tttcaccttt
    30001 ccaaaccaag gctcagctat tcaagtagat aacattatgt tatgatagca aagaaatgta
    30061 ttaagaaaac ctttcactat agagggagaa aaaaatgaac gatttattag caactatatt
    30121 tacaggtatt attattgatg aaaatgaaaa aaactacttt gtccaaaaaa acggacaaac
    30181 ctttggctta agcaaatcag aaggtgaaca caaaatcggt gaagcagttg aaggattcgg
    30241 ttaccaaaat caaaaacatg aaaatcggat cactacacaa attcctaaaa gccgtatcgg
    30301 tcattacgct tttggaatcg ttactgctac acgaaaagat ttaggtgcgt ttgtcgatat
    30361 cggtcttcca gataaagact ttgtcgtatc tcttgatgaa ttgccgacaa tgacagagct
    30421 atggcctaaa aaaggggatc gcttgatgat ttcccttaga gttgatgcaa aagaccgtat
    30481 ttggggcagt ttagcagatg aaaaaatatt caaatcttta agcaaatatg gaacagaaga
    30541 aatgaaaaac aaagatgtga taggaacagc ataccgcttg aaattaacag gtacgtacgt
    30601 tttaacggaa gatttctatc ttgggtttat ccatccttct gaacgatata atgaacctcg
    30661 tctaggcgag cagataaaag gtcgagtgat tggtgtccga ccagacggtg tactgaatct
    30721 ttcattgaag ccaagagcat atgaagctat atcagatgat gcacaaatga ttttaaccat
    30781 tttagaacgt tctgcagatc agcaaatcaa ctttacagat aaatcggatc cagaagaaat
    30841 catgaaagct tttggtatca gcaaaggagc atttaagcgc gcattaggta atcttttgaa
    30901 acaaggattg attacacaag aaaacggtgt gacaaaacta gtcaaaaaat ctaattgaga
    30961 atcccttgca tgtttttctt aactagctta taataatttt aattaacata gtgttatatc
    31021 tttttttatt tgtaattatt ataaattgtt ggggttgagt aaatggtcat gaatgctatt
    31081 tcggcgatga aaaaaacaaa acagcagcta catgaagcgg ggtttaagct gacaccgcaa
    31141 agagaagcta ctctgcttgt gcttttggaa aatgagaaag agcatttatc tgcagaagaa
    31201 atcttctttt tagtgaaaaa aaagagtccg gaaattggac ttgctactgt ttatcgaacc
    31261 cttgaaatct tgacagaatt gaaaattgtc gataaagtaa gttttaatga tggattagcc
    31321 cgttatgatt tgagaaaaga aggcgccaaa catttccatc atcatttact atgcttgaac
    31381 tgcggcacga tcgaggaagt agaagaggat cttttacttg aagtggagca agtagtagaa
    31441 gaacgttatc attttttagt aaaagatcat cggctaacgt tccatggaat ctgtcaggaa
    31501 tgctatgaaa aagaaaaaag
//
LOCUS       DACDLI010000027        30849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDLI010000027 DACDLI010000000
VERSION     DACDLI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.027"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            516..659
                     /locus_tag="IV353_001282"
     CDS             516..659
                     /locus_tag="IV353_001282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9009545.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            726..1237
                     /locus_tag="IV353_001283"
                     /pseudo
     CDS             726..1237
                     /locus_tag="IV353_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1381..2670
                     /locus_tag="IV353_001284"
     CDS             1381..2670
                     /locus_tag="IV353_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9009546.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            2746..3627
                     /locus_tag="IV353_001285"
     CDS             2746..3627
                     /locus_tag="IV353_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9009547.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            3617..4432
                     /locus_tag="IV353_001286"
     CDS             3617..4432
                     /locus_tag="IV353_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9009548.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            4448..5572
                     /locus_tag="IV353_001287"
     CDS             4448..5572
                     /locus_tag="IV353_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009549.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            5550..6353
                     /locus_tag="IV353_001288"
     CDS             5550..6353
                     /locus_tag="IV353_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9009550.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            6804..7076
                     /locus_tag="IV353_001289"
     CDS             6804..7076
                     /locus_tag="IV353_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9009551.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            7060..7395
                     /locus_tag="IV353_001290"
     CDS             7060..7395
                     /locus_tag="IV353_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9009552.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(7574..8788)
                     /locus_tag="IV353_001291"
                     /pseudo
     CDS             complement(7574..8788)
                     /locus_tag="IV353_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            8989..10263
                     /locus_tag="IV353_001292"
     CDS             8989..10263
                     /locus_tag="IV353_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9009553.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(10646..11374)
                     /locus_tag="IV353_001293"
     CDS             complement(10646..11374)
                     /locus_tag="IV353_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9009554.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(11352..12926)
                     /locus_tag="IV353_001294"
     CDS             complement(11352..12926)
                     /locus_tag="IV353_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9009555.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            13155..14093
                     /locus_tag="IV353_001295"
     CDS             13155..14093
                     /locus_tag="IV353_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9009556.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            14105..15016
                     /locus_tag="IV353_001296"
     CDS             14105..15016
                     /locus_tag="IV353_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9009557.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            15040..16512
                     /locus_tag="IV353_001297"
     CDS             15040..16512
                     /locus_tag="IV353_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9009558.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            16563..17174
                     /locus_tag="IV353_001298"
     CDS             16563..17174
                     /locus_tag="IV353_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9009559.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            17204..19117
                     /locus_tag="IV353_001299"
     CDS             17204..19117
                     /locus_tag="IV353_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP9009560.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            19245..22226
                     /locus_tag="IV353_001300"
     CDS             19245..22226
                     /locus_tag="IV353_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP9009561.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            22223..23884
                     /locus_tag="IV353_001301"
     CDS             22223..23884
                     /locus_tag="IV353_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP9009562.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            23924..24856
                     /locus_tag="IV353_001302"
     CDS             23924..24856
                     /locus_tag="IV353_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9009563.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(25215..26780)
                     /gene="guaA"
                     /locus_tag="IV353_001303"
     CDS             complement(25215..26780)
                     /gene="guaA"
                     /locus_tag="IV353_001303"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9009564.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(26835..28109)
                     /locus_tag="IV353_001304"
     CDS             complement(26835..28109)
                     /locus_tag="IV353_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9009565.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            28397..29050
                     /locus_tag="IV353_001305"
     CDS             28397..29050
                     /locus_tag="IV353_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9009566.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            29047..29364
                     /locus_tag="IV353_001306"
                     /pseudo
     CDS             29047..29364
                     /locus_tag="IV353_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(29412..30584)
                     /locus_tag="IV353_001307"
     CDS             complement(29412..30584)
                     /locus_tag="IV353_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP9009567.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         9849 a         5058 c         6237 g         9705 t
ORIGIN      
        1 ctgtctgtga ggacagcttt ttttgtgggc taggcatagc aaaaaggggt agtgacagtc
       61 cactgggagg gtgcacatcg accaatcgct agagaaacgc aaggtggtat ttgtgagaag
      121 atgacggaag aaatcctgta tcaaagactt ggttcaacga gcagaaatct gatagtaagt
      181 ctcggtcatt caatgggaat aagccattga acaccttgat ggtatcctgt aagaaagaac
      241 ctcaaggact ttcagcaaga agaaggaata gctgaaaaca aaaaaaggtg tagtaggtta
      301 aaagcgggga atgtagaact acttaattgg tgaaactagc tagaagggca aaatcgacga
      361 ttcatgagtt cgtaggaagg agcatgcaag atttacaact agtttactga aaaaacgcag
      421 ggaatttaca agaaattaac aagaaattaa cgcttcatcg gtatagtgga cgtgtgggag
      481 aaaatcacgg aattaaagga ggggtcaaaa tgaccatgat cactgcacac tctggtagtg
      541 acaatacgga ggagaatagc tgggcatttc ttcgcttttg tttggatcgc cagctttgtt
      601 ttgaagtaga tgtgcgtcag caaacagatg gtacgctaat actagcccat gacaggtaag
      661 aagttgtccc agattcggta acccttgagc aggtgtttca agcagtggcc aatgagaagg
      721 caaccatgac cattaactgt gatttaaaag aagagggatt ggaaatggcc gttttttcgc
      781 tggcacaaac ctatcaatct ggccacaaat tgttcttagt ggctatgtac aattatcttg
      841 ggctgcgatt tggggaaagc atatatttta taatattgaa aatcagttcg aagggaaaaa
      901 acttggggat attaccgaag cagatttgat cgatacctta cagaaattag cctatcaaag
      961 cgttcaaatc gtcaatattc atgaaggatg gttaactaag ttggcccgtg aagtggggca
     1021 gcagctagga ttgacatttt ctgtatggac agtcaatgaa ctagaaacga ttgagtacct
     1081 tgcgcgctgt ggtgtttata atataacctc acgttgtgca tgggcctatt tatggaagaa
     1141 aggagcacag aataaactat tttataatca aaacagagga gttaagagat ttggagatgg
     1201 gatacatgac catatggggg ctttattctt agcttgatgg atatgtccag tgaccctgta
     1261 ccccgaaatg cacaaatttc gaattattta cagaaaatta actagaaatt tacattggta
     1321 gttcataata tttcttgatt agaaaaatgg ccataaagaa attaggagga agttaatgtt
     1381 atgttaaaga aaaatcttgt tataggggtt gtgtctgttt taagtgtcat ctctttggca
     1441 gcttgtggta gtgggaatac caagacaaca aaaaaatcgg agggtcgtgt aacgatagag
     1501 tattggcatg ttaatgcgga taccctaggt ggaaaaacag tgaatgaatt agtagataaa
     1561 tacaatcaat ctcaagacaa agtgaaggtt gtagcaaaat ataatcctga tatgtataaa
     1621 ggcttgatgc aaaatttaca agcttctgtt gcctccaaag aaataccgga cgtagtacaa
     1681 gtgggatggg cttttaaaga ttattttgca gaaaatttca aatatactaa tcctcaatct
     1741 ttagtagaag aagtggatcc ggataatgct gattatttta aagatcattt tttagacaat
     1801 atcatggcac tgtctgaaaa agatggtaaa caagtgggta ttccttactc cattagtaat
     1861 ccggttttat atttaaatca agatatatta aaagcagcgg gcttggatga aaatggtcca
     1921 aaaacttggg aagaagttca aactttttct aagataataa aagaaaaaac tggaaagtat
     1981 ggcgtgtata ttcaagaacc tgctgataat tgggcacaac aagcaatttt agaaagtaat
     2041 ggggcaagtg taataaaaga tggcaaagct acctttgctt ctaaagatgg tatcgctgct
     2101 tatgaattat atcaaaggat ggtaaaagac cagacggcat tacacactac gtgggaacaa
     2161 ggtgtgcaaa gctttattga tggtaacgtc gcgatggtct ataccactat tgctcaaagg
     2221 gacaacattc aaaaaaatag tacttttact gctaaagcag ttgagtcacc aagttgggaa
     2281 gggaaaaaag ttaaactacc tgctggtggc gcaatgttag ctgttacatc acaaacggat
     2341 aagaaaaagg aagcaacttg ggatttctta aaatatcttt atagtgttga atctttagct
     2401 aaatggacag aaggtactgg ttatttacca ccacgtaaag atgttgcaga cgcccaaaat
     2461 ggattaaaag ctttcttagc tgaaaatcag atgatgatac cagcaattga tcagatggat
     2521 aagatggtca gttggagttc atttcctgga gatgctggat tagaagctga acaaaaattg
     2581 ttggatatgc gggataaaat tttatcaggt aattccgtca atgaaacgat gaaagctact
     2641 caaaaggaaa ttaatgagtt actcaaataa gcaaagcaag cagacgacag cgcctgtaag
     2701 aaaggaaaaa caaggtcgtc tgcttttgac ataaggatga ggaggctgtc ggaaatgaag
     2761 cgctatgtag gttatttgta tctggcgcca gcaattattt tgttgggcgt gtttgttttt
     2821 tatccattga tttatacgat ctatttgagt ttttttgact ggaatatggt ggcccccact
     2881 aaagaatttg ttggctttgc caactatgta aaggtgctga ctgatccagt ctttcataaa
     2941 gtgttgaaga atactttgtc ctataccgtt ctatttgtct tgctgagctg tgtgttccca
     3001 tatattacat cctttttatt aggaatagtt atagaaaaag ggcagaattt gtataaacca
     3061 ttatttttta ttccagctgt gatttctttg gtagtggcgg ggatggtttt tacatggata
     3121 ttaaatccgg tatcgggacc aattgctgtc ttgttgggaa attttggttt aagactgcct
     3181 atgtggatga atttacaagg ttgggtaatt gctgtgattg caggtattac tgcttggaaa
     3241 gtttttggtt ataatttcat tgtcattttt gcagcaattg tgggaattga tcaagagatg
     3301 atcgaagcag ctaagttgga tcgtgtaccc atctggcgca ttttttttac aattgtgttg
     3361 cctatgagta gttcgacagg actatattta ttggtgatga ccattgttca aggtctgcag
     3421 tatgtgttta ctccgattaa gatcatcact cgtggtggac cgaataatgg tagtgcaaac
     3481 gtgatctatt atgcttatca aaaggctttt gaaatgtata aagtcggaga atcttcagca
     3541 atttcagttt taactttatt aatatttgct gttttcttag gggtaatatt taaatttgtg
     3601 gaaaaggggg tttactatga aaattaaacg ctggttgcca catctcttat ttatgttcat
     3661 cataatcatt attatctatc cattattgtt tattatggcc aattctttta agccattgaa
     3721 agatgcctat cagacgatta ctagtttgat accacagccg tttaccttgg aaaatttttg
     3781 ctatttgttc gaaaatcttc caattatcaa aattacgaga aatacttttt ttattgctac
     3841 aattataacg gttgttaagt taggcgtagc tttttttgcg gcatatagtt ttacttattg
     3901 ccgaatcaaa gggaagaaaa tctggtactt tatcatcatt ggtacgatgt tcattccttt
     3961 tactgttgcg atgattccta attatttgtt gattgcaaaa cttaatttaa ttgatactac
     4021 ttggggtgtg ataataccac aatttgctga tgcgatgggc atttttttat tgacgcaaac
     4081 aatgcgggaa attccagtga gccttctaga tgtggcggca ttggataaca tcaaacaaac
     4141 aaccgtcatg agtaaaattg tttttcctct tactagacat gcgattactt ctaccggtat
     4201 ttggtttttt attacctctt ggaatgagta tgtttggccg gtattaattt tacggacagt
     4261 agataattat actttgccgt tggcgatgca gacctatatt agttctgaag gtgggacaaa
     4321 ttttaccgta gcaatggcga ttagtttaat tacaatgttt gtaccgttag tcttgtatgc
     4381 aatttttcaa aaatatatta tcgggacttt tgtttctgca ggtattaaat aaattggagg
     4441 acaaatcatg ggagaaacgc ttgaattaag aaatatcgga aaaaaatatg gagaacatac
     4501 cattttaaaa gaaattgatt tagctgtggc cgctggagaa cgaattgtgt tactgggacc
     4561 atctggttca ggaaaatcta ctttgctgag aatgattgct ggtttggaaa aaattacgag
     4621 tggagagctt tttttaggta cacaactagc aaataagcta ccttgtggag aacgagagat
     4681 cgcaatggtt tttcaaaatt atgctttata tcctcacatg actgtaaagg agaatattat
     4741 ttttgcatta agggcgaata aaattaagac agctgacatt cagcaccgtt tggaagaagc
     4801 tttgaagatg ttaggtttaa cgccatacca agatcgactt cctaaagatt tgtcaggagg
     4861 gcaacggcag cgtgtagcat tggctcgtgc ggtggttaaa cggagtaatt attttttatt
     4921 agatgaaccg ttatcaaatt tggatgtgcg tttacgattg gatgcacgaa aagaattggt
     4981 aaaaattcat caacgttata aacaaacttt tgtctacgtc actcatgatc aaattgaagc
     5041 gatgacgtta gctgaccgga ttgttttatt gaatgaaggt agaattcaga tgattgatac
     5101 gccgaaaaat gtttatacga aacctaaaaa tacttttact gcaaatttta ttggttcgcc
     5161 aggcatgaat attatgccag ttacttatga tcaaggaaca ttaaaggtag ccagtcaatt
     5221 tttaacattg aatccagctt ggcaaaaaca acttcttcag gttagtggct cattgtattt
     5281 aggcataaga ccagaacacg taaccttttc aaaagaaaca agcgatttac acggtcaagt
     5341 gaagtatcgt gagctattag gtcaaagtta tgcgtacacc gttaaagtcg atggtataga
     5401 aatgattgtc cttaatgaaa aacctacttg gcagattggt gatgcagtca atctccgcat
     5461 agaacctgaa agaatgcatt tttttgatta tcagacgcaa gaaaatttag gatatcctga
     5521 ttattttatt gaagaaagag ggaatagcca tgcgcttttt gcatctctct gatatccatt
     5581 ttagagaaaa ctatcaaact aacttgttta ctgctgaact atttccaaca attaaaaatc
     5641 cacagttggc attggaaaaa ttgttacaag aactctcgtt aacagtgtac gattttattc
     5701 tattaacagg tgatttagta catgaagggg ggatttctga ttatcaagtc ttacggaaaa
     5761 tgttggatca atatttccca accacacctt actatttttg tcgtgggaat catgatcgtc
     5821 gcagtgtttt ctttgaagga atgagtgttg aaagcaatga aacgggagag tatatccatt
     5881 gtacgaatca aaatgggtta cgaattattt ccttggattc tgctcaagat acccagcacg
     5941 aaggaaaaat atcgatagga cagatgattc aattacaaga gtggttgggt acacctgctg
     6001 aaaagggaac gattttattg gttcatcatc cattgtcgtg ggaagatcca tctattgtaa
     6061 ctgaagttcc ggaaggtttt gctgaattga ttgccgaaag tgatatttta ggtatttttg
     6121 taggccatat ccatcaaggt agcagtgctg aatttgctgg taaaaaacag tttatggctg
     6181 aatctttatc atttggtgta gatgaatacc cagactattc tctttttacc aatagaacag
     6241 gattcactag tggcagttta attgacggaa aaatttttac ttatcagcat tatttaactc
     6301 cacatcaaat agaaataggg cgcatgaaaa aaccaattac tggtaatcat taaaaattat
     6361 tgattaaaaa taaagacttc attcactttt tagccgtggt gctcaatgtc tggttccact
     6421 tgaagaactt ggaaaatcgc tatcaataaa ccactatccc tccaattaca aaaagggtgg
     6481 agaaagtttt ccaagaacca tatcggtctt cgtggttgtg ccattcgttt tttaccagag
     6541 cataccacat ctttgtggag ttttcagaca cgtcctagtt aatttggttc ttttaggagt
     6601 cttttcctgt tctgtgtgtc gttgagtgtt cgcttcaatt atcgctgtgg cggattttga
     6661 aacttttcta tctatcaagg ccatttagca ctatatcctt tccttatatt tagataaata
     6721 cactgcattt caaggagttc aaaagtagtt gtattaattc catttgaact ctttccgtga
     6781 tataatgtgt ttgtcagaaa gaggtggaga aaatggctaa gttacaaatt tctatggacg
     6841 aaaaattgaa aaatgaagca gaaagcatta ttgatgaact aggcatgagc cccaaaacag
     6901 ccgtcaccgt tttttataag caaattgttg agcaaggaaa aatcccattt tctattgaat
     6961 tgtcggaaaa aagtaaacga atccataata ttcaaagatt agcggaaaaa ttgcctgtcc
     7021 aaaaactaga cactgacgaa aaaattgaag aatggttcaa tgaagatgaa tactaatgat
     7081 atagcaaccg tctacgttgc tttcacagct gcagagggcg gtaaacgccg gcctgtctat
     7141 attctaaaaa atggactgga cacaatttcg tttctgtcaa ttacttcaaa atatcatact
     7201 aaatccgata aaataaagct gaaatacgtt gaaataaggg attggaaaca ggcaggacta
     7261 aaaaaagaat cttggattga cgtaggaact ctaaacgaat tacccaaaaa caacaaaaag
     7321 gtcaaatgga aaaaaattgg ggaattaagt ccaagcgatt tacagcgact ggaagaacgc
     7381 atggaagaat tataataata ctaaaaagaa gggctctttg tcaataagga ctgatgggtt
     7441 gtacaaaatc aaatctgggt cagaatgaac ctcattctgg ctcagatttt tggctctttg
     7501 tcaataagga ctgatgagtt gtgcaaaatc aaatccgggt cagaatgaac cacattctgg
     7561 ttcagatttt ttgttatctt acattgatta attgattgat caaaaagatt ctaattctca
     7621 tgttctcgaa tgatttaaat ccgtaggata ctcgtttcat tgtctttatg tgggtattct
     7681 tcgcttcaat ttttccattg gaataaggat agatcattgc gttggtgata ccttcttcat
     7741 aggtcagaag gttttgaagc ttctcccgaa agccgtcatc taacgtttcg ggaagttctg
     7801 ccaataagga gaaaaataag tcagggtctt tgtctcgaaa ggcttcgact aattcatgaa
     7861 aaaagggata cgcctccttt agaggcgcag aaaactcaag caatcgatca atcatcattg
     7921 cttcagtgag aaatgggtat tttggtgctc ggaaactttt ccatgttttg tactcataat
     7981 ggttgatgct tgcacgattt tttagcagaa agcgccagtt ctttttcagt ttttctgcct
     8041 gacttttctg tcctgcttta cgaagctcat tcatttcacg gatacgcaac tcattgagtg
     8101 cctgattcat gtgtttgaca atatgaaacc gatcaatcac tactttcgca tttggcagaa
     8161 cacgtttggt aagctggaag taagcggcgt tcatgtctgt gactaagaat tctacttctt
     8221 ctggattggt gcagcctaag aaatagcttg ttaatcgagg taatttacgc gtaggcaaga
     8281 cagcaattaa gtttcctgtt tcaccatctg cgcaaataaa gctcatttta tcttctatgg
     8341 aagcgtgcga acgaaattca tcaaccatca atactctggg gagaatcttc ttagaaggct
     8401 ttggtaaata gcttttaaac tctttcaatg tacgagtaac ggtagtcaaa gatacctgac
     8461 aacttttcgc aataaaagac aaagatactt tttcagtcag taaagaagca attttatatc
     8521 gaacatgatc tgcgattgaa tggctgagtc ggacaaaata actttgagcc gtccaatggg
     8581 tacgacagtt tttacaggta tagcgttgct tttttaggcg cgtaaccaaa ggcatatgat
     8641 tgtattgttc aaaacggaca atcgtttcct tcttcccatt tttcactata attgctttcc
     8701 cgtttccatc taccacagta gaaccacaac ttctacaagc acgaggagta ggcgagagaa
     8761 cagcatcgac gaccaacgtc tttttcttgg ctctttgtca aataggactg atagagtgat
     8821 ataagaacat ctaaggtaga attgattttg cttctataat attttgtgtt ggtggaaaat
     8881 cgaaggcgat tttctactaa cactttttct tcgtttcaac tcaaaaagag actcactttt
     8941 ggtaaaattt agtcgaccaa aacgtcatcc taccaaagga gtctctcaat ggataatcat
     9001 actagaaaat tactcggatt aacagataaa catttatttt ttgatgaaga atggctgatt
     9061 gaaaaagaat ataaaggcgt tcaagcgcag tttattaaag gaaaattgga tgactctcct
     9121 tcctattgtg aacactgtcg atcggttcgt attatccgca acggttccta tacgacacgc
     9181 actcagatat tgaaagtcaa agaaaagtta actattttag aattaaaacg tacccgtttt
     9241 ctctgccatg attgtggaag gaccttttct gcgaaaacgg acttagttga tgaacatcat
     9301 caactaacaa aggaactgaa gcaggcaatt ctcatggaac tgtacgaaaa tcaatcacgt
     9361 aaactaatcg ctaagaagta cttcgtctcg gatgggacgg tgacacggat cttgcgtgaa
     9421 gcaacgaagc attaccagtc acgtatgaac ttccttccga cggtgttgtg tatggatgag
     9481 ttcaaatcaa tgaagtctgt ttctggttcc atgagtttta tttgtgttga tgggacaacc
     9541 catcagctat ttacgatcct tgaagatcgc agactttaca aactaactca atacttcctg
     9601 cactttccta gaaaagcccg tttaaaggtc aagtacctag tgatggacat gaatgccagc
     9661 tattgtcagt tgcttaaaac agtctttcca aatgcggaga tcgtaactga ccgctttcat
     9721 attgtccaac acatcaatcg cagcttcaat caattacgtg tccagatcat gaatcgtttt
     9781 cgaacctcac actctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc
     9841 ttactcaaag attccacgac atgggagcca ctcaaacggc attatcatcg actgtttaaa
     9901 cgaccaatta gtcaaacaga aatcgttgat gagctgttaa gctacaacga agaattgcga
     9961 actgcgtatc atttttgtca attattacgc tattatttcg ttaaacgagg tacggaaggc
    10021 tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa aaagaaattc
    10081 acgatcttcc attgctacca atcgggtatt tcaaatgcct ttaaggtgtc ccactcaaac
    10141 ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc gtttggttat
    10201 cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat ctttgaaaac
    10261 taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta ttcaaatttg
    10321 tcttcaccaa cacgatttga cgaagagcct atttaaataa ctacatccat ctattctatc
    10381 ttctaaaata gcctttttca tttcagttaa atagttcagc gccatttttg cactcgactt
    10441 gcatagtagt tttttccata ttcctcaacc tcagcggggg ttgaagacat aaggaaaatc
    10501 ctatgcgtta tgctcaactg gatctgatta aatattctaa tgtatttttc tctaccatca
    10561 ttaaatggtt aatagccaaa aatcaccact tttcaatggc tccttgttgt ataagtataa
    10621 ctagaagatg aacacgtctg agaaattata gaagcttttt acggtaatta ctggggctgt
    10681 gtccagtagt ttttttgaag agaacagaaa aatagctcac gtgatctatt cctacttttt
    10741 gactaatttc atcaatagat aagaatgtgt ctttcagtag agcacttgct cttttgattc
    10801 gaaagtctag caagtaatca tgaaaggaat aacctgtttc ttttttgaag gcccgtccta
    10861 aataagcggg atggtaatgg aattgtgtag ctacatcttt gagcgtaagc ttttcactat
    10921 aattgttttg aacgtaatca atcacttgtt gaattgactc attaaaagaa tcatcagtta
    10981 gagagttttg attagctgct gatttacgca ttttttcttc ttgtactaac ttaactaaat
    11041 aaggaaaatc aatgggtttc gttatgtaac ttttaataga taaatcaatc cccttttttg
    11101 catactgaaa attttggtgg gcactaatga caatactagt aaagtagcga ttggatgttt
    11161 gttgcaaaga ttcgattaat tcgagtccag aaattttccc catcgtaata tctgtgataa
    11221 gtaaatcaaa gcgctggttt tgcaggtttt taaatgcttc gtctccgtct gtgtagcagt
    11281 gatcgataat gatgtcgggg actttcttca aaagataatg ccttatgcct tctacaattg
    11341 tacaatcatc gtcaacaatt gcaattctca acatgcatat tccccccttt ttgtattttg
    11401 atagtaatcg ttgttccgac gtgtagttgg ctttctataa caaaacgtgt atctccagga
    11461 tagagatgct ttatacgtcg aaaagaattt gagatgccga tattctcact ataaggaata
    11521 tcatcattaa gtttttgttg aatatgctcc agcttgtctt ggtccattcc tttcccattt
    11581 tcaacgattt ttaacacata atggttggtt gtttcagttg catagacagt caaacgattt
    11641 tccttttttg atgaatcgaa gccgtgttta atataatttt caacaaatgg ctgtaaagaa
    11701 agttttggta acattaattg atcaatatga tcaatcagct gatagtcaaa gttatgtcga
    11761 taacgacgtt gatgtagttg caaggagagg tcgatattaa ttaattcctc tttcaaatca
    11821 attatgtcgg gctgatatgt attattgcga tacaacttgc tcaataaata tatactgtcg
    11881 ctgatttctt cgtcatcatt caatatggca gtcattcgaa taaattccaa attattaaat
    11941 agaaaatgag gctcgatttg atattgaagt gctgcatact ttgcttcttg cttcaaccaa
    12001 ttattttcat aaagtgtttt ttgatatgta gcattatcta aataaaactg attgatatgc
    12061 atagcgattt ctttcaaatc accgactttg ttttcgatat tgattgactt gtagtctcct
    12121 gtagatgaat gatttaagga agtaatgatt tcctgatact gctggatata gttacgaaaa
    12181 cttctattta ataattgatg aatagcaaga ataaacaaac tcattaataa gaaaatcatg
    12241 attgccagac ctaataaggt ttgtgacgaa taatttggat tttcatatac ttgtaatgtg
    12301 tggttaccga tagctatctg ttgcgttttt ttccatttag aaggcgagat gtcttcagat
    12361 aaattgacgg aatcaatacc attagacaaa gaataattgt aatgtgttgg aaagagactc
    12421 ttttttagcg cgttgatcga aacgtctaga atcaatgtag catcgggcat gtcctcacta
    12481 atgaaactta atgaacgaat aaaagaaatt ttttcgtttt ttgtaggtgc gtaggcactt
    12541 aagtattttt cgtaatctaa tgagatagat atatttttaa cttcatcatc ttgcacaagt
    12601 ttttttaagt tatctactaa ataggtaggg gagcgatcct ttgacaaaat atcttccatg
    12661 tattcagcag gtgatgagag gtagtagtca ttaaggtttt cttggaaatc ataattttga
    12721 tagatctctg tcacgatacc ggagagaata cgattatatg tttccatata gtgggtgatt
    12781 tgctgaatgt tttgcaggtt ttgatttttt tctttttcaa tcacattgat ataggaaaag
    12841 aacaatgtga aacaggcaag taaagtaata aaaaaaatca atatgtatgt aaaacgcata
    12901 ataacttttg ataaatcgtt tttcattcaa agtcctcatt ttgataatct ctaattttat
    12961 gaatcattat agcacatatt acaagtgatg taaacgcttt gcaaacaggg ctacttatga
    13021 ttgtgaatac agtttccgtt taaaaactat tctattgtag aggtttaata aactaaaaat
    13081 aatgtataaa aaattaatgt aagcgattaa ccaagtgggt ataatcaagg aaacgctgag
    13141 agaggagaga caaaatgaag ctaaagaaag ggttattagg aaatctaaaa aaaaaccgag
    13201 cattgtttat tatgctttta ccaggtgcgt tatggttatt tatattttgc tatataccaa
    13261 tgtttggagt tgtaatcgct tttaaagatt tcagaatttc atcaagggga ttctttacaa
    13321 gtttgttcaa cagtccttgg gttggctggg aaaatttcaa atatctcttt gcttcgcaag
    13381 atgcttggat cattactcgc aatacggttg tttataacgt tgtaggtatt ttgctaagta
    13441 ctttttgtac agtgtcatta gcaatcattc tgagccaaat ggcgaacaaa cggttattga
    13501 aaaagatgca gacatttatg atttttccta attttttatc atgggtagtc atcagttact
    13561 ttgtttacgc atttttgagt acggataaag gattaattaa tattttacta agcaattttg
    13621 gtgtggaacc cataaattgg tatagtgaac cgaagtactg gccttttatt attgttttta
    13681 cgagtttatg gaaaggcgta ggctatggat ccatcattta ttacgcgtca attatgggga
    13741 tcgatactag ttattatgaa gctgctttag ttgatggtgc cagtaaatgg caacagataa
    13801 ggaatgtagt cttgccacat ttaagatcga ttattgtcat tatgttgatt ttgtctgttg
    13861 gtagtatttt tcgagctgat tttggattgt tttatcaatt gccaagaaat tcgggggctc
    13921 tttatgatgt gacgaatgta ttagatactt atgtctaccg tggattaatg aatagcggag
    13981 atatcggaat gtctacggca gcgggattat atcagtcatc agttggcttt gtcttgattt
    14041 tgattactaa taagatagtc aacaagttag atcctagttc agcactattt taggaggaat
    14101 caaaatgata tcaaaattaa aacggcagat gtacttttcg ctgccagtcg aacggttttt
    14161 cacaatatta ctaatcttat ttactcttag ctgtattttt ccctttattt tcgtaatcgt
    14221 catttcatta acagatgaaa aagcattaca actcaacggt tacgaattat tgccggctca
    14281 atggagcact gatgcatatc aatacttaat acaagatggc ggacagttac ttcggtcatt
    14341 aggcgttaca attatgatta cagtgattgg aacgttgatt actgttttta tgacgggaac
    14401 ctatgcttat gttttatcaa gagcctcttt cccttaccgg aagttcttta ctttttatct
    14461 tttttttacc atgttatttg cgggagggat ggttccttct tacttagtga tgacgaagat
    14521 gcttggctta aaaaatacaa tttgggcttt gattctacct cttgctttta gcccatacaa
    14581 tgtcattatt ttaaggacat tctttaagaa gtcgattcca gaatcgatta tcgaatccgc
    14641 taaaatggat ggctgtagtg agtttcgagt ttttttccaa atcgttttgc ctttagcgat
    14701 cccaggggtt gcaacaatag gtttattttc aagcttaggt tattggaatg actggttcaa
    14761 cgcattgctg tatattgaca ccaacaaatt gattccattg caatatttgt taatgaagat
    14821 tcaaaattca atggaattct tagcaaataa taatgacatt acattagcgc agcaacaagc
    14881 aattcaaaat agccttcctc aagaatcaac acgaatggca atggtcgtag tagctacttt
    14941 accgattgca attgtttatc cgttcttcca aaaatacttt gttcaaggat tgacgatcgg
    15001 tggagtgaaa gaataaaaaa gaataggaga tgttaaaaga tgaaaaagaa catgcagaaa
    15061 tatttgattg gtggtgcttc tgtactaacc cttggcctat taggtgcttg tggtagcagt
    15121 aatggcgaag caaataatga aacaggaacc acaaaagtta cttggtatca ggttggagaa
    15181 gaacccaaag attttgatga ggtcatgaca aaagtcaacg aaaaattaaa ggaagaaatc
    15241 aatgtagagt tggatatgcg gctgattccg gacggggatt atcagcaaaa gcttggtgtg
    15301 atgatcaatt caggagaaga atatgatatt tgtttcgtca atggcacaga ttatgtgaat
    15361 tatgggaaca aaggtgcttt cattagtctc aacgatcatt tggataaaga gttgaaagat
    15421 tatgcagctg aattgaatga aggatttatt gaaggtgggg caattaatgg cgaaacttat
    15481 ggattgccag tcaataacga tattgccaca acggaatatt ggacctttaa taaagatttg
    15541 gtagatcaat accaattgga tgtagagaat gttgaaggtg tagaaacatt ggctccagta
    15601 ttagaaaagt ttgcagaaaa aaatactgat acgaatatta cgccaatcgg tatgacccgt
    15661 accagtgtct gtattcctta tgacaatatt atcggtggtg gtgtaccgct agcggttgca
    15721 tttgaaggcg atacacaaac agttgttaat acttatgaga ctccagaaat gatgacttat
    15781 ttgaaggcta tgcatgagtt ttataaaaaa ggatatattt tgaaagatgc agccactgtt
    15841 acgacagatc catttcgaat tgatagtaaa aactggtttt taggtcgtaa ctcaagtggt
    15901 tatcaaccat tagcacaaaa aaaattagaa gctctagcgg gacatggatt aattttaaaa
    15961 ccagctggaa aagcttttaa aacgactgga tttgcaagag tggcaatgca tgcggtatct
    16021 gcttcaagta aacatccgga agcagccttg aaattgttga attatgtgaa taccaatcaa
    16081 gaagtagcgg atttaatggt ttatggaatt gaaggaaaac actatgatcg caatgaagat
    16141 ggttcgatta ctttaacaaa ctctgatgat tattatcaag gcgcttgggc attggtaaat
    16201 acttatctag tttcaaaaac ttcaccgatt acagaagaaa gtaaaaaact agaagaagaa
    16261 agtaaaaagt ttcaaaatga agcaccgatg tctcctattt taggtttctc ttttgacgct
    16321 tctgattttc gaacagaaat tgcaagtgta caaaatatcg tgtcacaata tgaagtactt
    16381 ttaagctcgg gaacagttga ccctgaacaa tatgtaccag agttgttaga aaaactagaa
    16441 aaagctggat tggataaaat tcaggcatct gctcaagaac aatacgatga atggcgaaaa
    16501 tctaacaaat aattttttac taaatgaaaa gaatagacta agcagttagt ggggggattc
    16561 aaatgaacga aaacaaatgg accaaaggat ttaattgggt ttttcaattg ttgttactaa
    16621 actttactta ttttatacta atttgtttag gatttggaat tttaggtttt ttccctgcta
    16681 cgatagcttt gattgctggc gttgtgaatc gaaaaaaata tggcttgcaa gagtctgtga
    16741 agttcatgag taaaatctat aagaaaaatt ttgttagata taatctatat tgctggtcag
    16801 caactttgtt gttggccggc aatattacta cgatctggct ttgttggcag caaagcgcac
    16861 cattgtttta tttattaagt acattattat cggtaattac actgttccta gtctttgcaa
    16921 tgttgaattg ggtcgtgatc aaaagtcttg ggttgtggat gatgggagac cgactgattg
    16981 actatttgct tttcacagca acgatgttgc caacggtagt catgcaaggc ttggtgcttg
    17041 gaatttattt ccgaatattg atgattttgc ctgtgatttt tctgtttggc ggaacagctg
    17101 taacggtaat attgattagt aaattaactg aaagagcgtt agcaaagttg ccttgtcaga
    17161 tacaatcgca ctaatttttt cacaaaaagg agtatttctt tctatgaagt taactgataa
    17221 acaagtagct aatatagcct ttatccagca acttgagatt ccattgacac aaaaagatga
    17281 agaacaaata gacttgattt ttactgacac aaaggctaat gtgcgacaaa tcaagattac
    17341 taaaaatgag cagcaacgaa tttgcttgtc ttattcacat gaagacttgc tgtatcgagg
    17401 attgtttatc tgccttccac gttttttagc tcaaggagga gagatgccat acattgaaga
    17461 aacctcgatc attgagacca gttattcatt agatgcttct cgtaatggcg tgatgaataa
    17521 agagactcta aaaaaattca tttcttactt agcgttgatg gggtacaaca cattgtatat
    17581 ttacacagaa gatacctatg aagtagaaga ccttccatat tttggttacc ttcgcggagc
    17641 gtatacaaaa gaagaagtga atgaaattgt tgaatttgct tctggttttg gcatagaagt
    17701 agttccatcg atccagaccc ttgctcactt gacccaattt ttgaagtggt atgctcaaca
    17761 agaactgatg gatgatcaaa atactctact gatcggtgaa gaaaaggttt atgcggcaat
    17821 cgaaaagatg attattgcct gcaaagaaat gtatcagaca gagcgtattc atttgggaat
    17881 ggatgaagcc tatcaagcag gtctgggacg atatttaagc ctgtatgggt attgtgatcg
    17941 gacaacttta atgattcaac atatggaccg tgtgttatca attgttgata aatataagat
    18001 gaaaccaatg atttggagtg atttcattta taagctatta gatgaaaccg gtaaagatag
    18061 gtattacaat cctgatttta aactcaaagc attagcagct aaaggattac cagcaggatt
    18121 aacctatgtt cattgggact atggttcaga tgaagtttcc tcctatgaga aagtaattga
    18181 taaccatttg aaattttgtg acattgatca ttacgtttta gctagtggtg cccatatctg
    18241 ggggagacta gccccgaatc atgggaaaac agaagcaatt gtttctgcag ctgtaaaagc
    18301 ttctaaaaat aaaggtgtac gttctgtgat gttaactact tggggagatg aagggcagga
    18361 aacagagcat tggcattcct taattggggc attaaaatta gcagattatg tctattctga
    18421 agaagagtgg aatgattttg aggaaatcta cgatcgttta ttaggaacag gtagttatca
    18481 attaatgtcc gaattacgat atttcgatga agtggatacc attacaaata acaacttaca
    18541 aatggcttcc gtgagtaaac tgctactttg gcaagatccg ttagttggta tatatgactt
    18601 tcatgtagcc gctcataaca aaattgccac tagaacaatt ggtacttatt atcatgagtt
    18661 agctcagaaa ttagatatga ataaaaattg gaatccaaaa caaccaattt taacaactat
    18721 tcgtcaacga tatatacttt tagctgaaat tttagcacaa aaggcagaac taggcatcta
    18781 tttacaacac tgctgtctgg tagaagacct tgcaggatta atggaattag cgaatcggtg
    18841 tgaagcacta gccaatcgag taaatgaatt ggcatctatt catgaaataa tttggtatca
    18901 aacatacaaa acctatggtt gggaagtatt agaacaacga tatgctgcat tagctcatcg
    18961 tctgaaaacc gttgcgcgtc gtattaaaaa tcatcttcag gggcaagatg ccctagagga
    19021 gcttttggaa gaacgattac cgttttgtca atatcaagtg ccgcttgaaa tttctggctt
    19081 taattatcgg caaagctcgg tgtcaggcta caattaacga aggaatcttc ccgttgaacg
    19141 gaccacttgc tcatctaatg ggaaagtggt tcgttttcta ttcataagta gcttaccact
    19201 aactctttta ttaagaagag aaaaaaagta ggaagtgaga aaatatgtta tcggactatt
    19261 atcaaaatct gtcagttata agtgtcaata cagaaccatt acgtaattat tatattcctt
    19321 atgcaagtaa agaagaagca aaacaagagt tagaaaggga aaaaacgagc cagtttatcg
    19381 atctcaatgg tacatgggat tttcagtatt atccttctgt ccaacagtta ccggaaaatt
    19441 tttttcaaga ggcctttact gaaagctatg atagaatttc tgttcctgga gtttggcaaa
    19501 cagagggatt tgacttacct cagtatatca atgtatctta tccaattcct tttgatccgc
    19561 cttatgtacc taatgatacc ccttgtggtg tatatcggcg tcagtttcaa attgagcaaa
    19621 cagatgcacg cttttactta aatctagaag gagcagatag taattgtttt gtttggataa
    19681 atagaaactt tgtaggctat gaccaagtga gtcatagtac atctgaattt gatgtgacca
    19741 agtatttaca ggttggggtt aatgaaattc aaattctagt gatgaagtgg tgtgatggta
    19801 cctactttga agatcaggat aagtggcgaa tgagcggtct atttcgtcca gtgtatcttc
    19861 ttcaacgacc aactatgcat ctgacaagtt atcaactacg gacgttgact gatcctaaaa
    19921 aaggaagcgg aacattaatg gtttcttttc aacgatttac tcctattgaa gtatcgttac
    19981 ctatttctta cgaattattc catcctgatg gacgtctcat agcgaaaggg gagtgttact
    20041 cggaacaaca aataaatttg gatgttgaag attgcctttt ttggacagca gaaacgccta
    20101 aattatatac attactttta acatgtaatg gtgaatattt taaagaaaaa gttggctttc
    20161 gagcactgac gatcaagaat cgtaagttat tgctgaacgg aacacctatt aaattatatg
    20221 gagtcaacta tcatgacgtg aatcagcaag gcacgccagt gatgacccga gagcagttac
    20281 gtgcagattt gttattaatg aaaaaattaa atgtgaatac tgtacgaacg gcacattacc
    20341 ctaaatcacc agatttttat gaattggcgg atgagattgg tctgtatgta atcaatgaag
    20401 cagatttgga gtgtcatggt gtggttaatc tatatgatac tgaagcgaat tatcaactaa
    20461 ttgctaatga tccgacattc gaaaagccga ttatggaccg agtgatccgt tctattcttc
    20521 cttatcaaaa tcgaagctct atcattatgt ggtctatggg gaatgaatct ggttatggag
    20581 taaattttga aaaagctcaa gctttagcac ggaaattgga tgattcacgg ttgattcata
    20641 atgaacgctc gattgaacca gttattgacc aagtgaatga ttttagtaac ttggatgtta
    20701 ttagtcaaat gtatccccca ctggaagatg ttcatcgtca ttgtcaaaga agtgatactg
    20761 aaaaaccttt ttttatgtgt gaatatagcc acgctatggg caacggtcct ggagatctta
    20821 aaacgtactt cgatttgatt caacagtatg attcctttct tggcggttgt gtctgggagt
    20881 ggactgatca tgcaatcaat agaggaactt tagaaacgcc atcttattta tacggtggtg
    20941 attttggtga aaaatatcac gacggcaatt tttgcgtaga tggtttattg accccagatc
    21001 ggcaattaac agtaggtgca ttggagtacc gtaatattca ttcgcctatt caactgattg
    21061 ctgtagatga ggagctgcaa gaagtaacct tgagcaatat aactgctttt acagccggtt
    21121 cagcatttaa cctttctctt gaatggtata tagatggaaa aaaaacagtt tatgaagtaa
    21181 tgaatccttt catacttgag cctggcaaaa gttgttcatt gcccatcaca gtacctacaa
    21241 cgattcatgg tgctcatacg gttgctgttt cgctatctgt ttataaaaat gatgagcttt
    21301 atggacagca acagctcttt cttaaaaaag aaaaaatgag attgccaaga actgctcatc
    21361 ataatgatgc accacaaatt tcagtaaaag gcacagagct gataatcaaa ggagaatcct
    21421 ttatttatat atatcaatta gcaaaagctg cgttcacttc tgtaaagatc aatcaaaagg
    21481 aacttttgga acgtcctact caattttcaa tttggcgtgc accaacggat aatgatcgta
    21541 acttagtaaa caaatggaaa gcagctggat atgctgaaat gtctttagaa atcagaaaaa
    21601 aagactggaa attcgtagat gatcatctcc tgatcgaaac gtcttatcga ggagttattc
    21661 caggattaca gccaattttt acaatgcaca tacagttgaa aataaatggt gcaggcgtaa
    21721 tcaaccaaaa agtatcagtg gagaaaaatc cgatttttcc agatttccct cgctttggga
    21781 tggactggcc gttgaatgat actttttcga aggtttctta ttgtggctat gggccgtatg
    21841 aaagttatcc tgataagcac tatgctgcga tgtttggtat ttatgagatg gagattgagg
    21901 aatttgatca gccatatatc aaaccacaag aatatggtaa ccgctggcaa tgtgattggg
    21961 tatcaatcaa aaatcatcaa ggatctacca tgacggttta tggaaactct aatttttctg
    22021 ctctgcctta tagtcaagct caattaaccg aaaagtcaca ccattatgaa ttaataaaag
    22081 aaaatagaac ttggttcaat ttaagttata aacaaaatgg tatcggctca cacagttgcg
    22141 gtccttattt agaagatcgt ttccgtttta tggaagatac ttttgactgg aatatagtga
    22201 ttactttcaa aggagaaaat agatgaaaga aacaaagatc aatcaagtca aaaaaaaatt
    22261 atctcgttat caagaagttg tttatcgaaa ccattgctta aaatggtcaa aaaataagct
    22321 gaatgttcgt tttaatggat tattttatcg tttcgaacag ttagaagaag tctggaaacc
    22381 ggtaacgaat ttccaatcat gtcctgtaca gctcataaat gatccaaaag aaacatacga
    22441 tttatgtata acacaccttt ataaccagca tttgaaaaat gacggatttt atttaaaggt
    22501 tactaatgac aatcaactta cgattgaatc agcaaacctt cgagggtttc aatatgctat
    22561 ggaagcctta ttagagagtt ttttttcggt aggagatcaa ttattattac cggaattgat
    22621 tctgaaacat gaaccatcag tgaaaattcg aggaatcatt gaagggtttt atggtatccc
    22681 ctggacacat gaaatgcgcc tctctctttt ttcatatatg aaagataacc aattgaatac
    22741 ttttatgtat gcccctaaag atgatgaatt attacgtaaa aaatggcgtg aactctacga
    22801 tgcgcaagaa ttagacaaat ttaaggagct tttatctgct gctgaggcta gcaatatcga
    22861 tttttggtac ttaattagtc ctggaaatga tattgatatt acgtgtgaag aagacattca
    22921 agttcttctg aaaaagttag aacaactgat tgagcttggc gtatttcaat ttggtctatt
    22981 aatggatgat attgactatc aattaaaagg tgctgctaaa cgccgttttc gagagccagc
    23041 attcgctcat gcctatttag tgaatcgagt agaggaatat cttagcactc ggttgtccaa
    23101 tcatgaatta gtcatttgtc ctacagaata tgataatcgt catggttctc gctatttagc
    23161 aacgttaagt caagaaatac cagaacaatt accgttcttt tggactggcc caagcacgtt
    23221 agcggcgtca atttctacag aagagctgca ggaaatggct tctgtctatc aacggccgat
    23281 gctgatttgg gataatattc cggtaaatga ttatttagag gataaagaac tattgttcat
    23341 gagtccatat gagaatcgaa caccgaatct ttctaaggag agataccagg ttacaggagt
    23401 agtttcgaat ccaatggctc aattagaagc ttcaaagttt acgatcaata gtatggctaa
    23461 ttacttatgg aactgtgagc gttttgatcc tttggaaact tggacgtcag tcgttgaaaa
    23521 agtagttgga agtaaactac aaccagctta tcttacctta accaatgctt ttccaaatca
    23581 ttacacaagc agtctgcttt cagatgaaga attactgcta gccaaagacc cagaatgggt
    23641 cacaaaagaa atggctgctt tagtccaagc agtaaaatgg atccagcaag aacaacatac
    23701 atctagattg agaacagaat tggagccttg gttacaagca atcacagaaa gttggacttt
    23761 ctggcaagaa tggcaattaa aaaaagatcc aaaagaagca gaagaatggt tggctaagaa
    23821 aaaaacacac cgcataggac gtgatttagt aactgcccat ttagagcaaa ttatcaaaag
    23881 ttagatatct taaaaaataa aaagcaagaa aaaggagaac aaaatggtca agagattaat
    23941 tagtgcaaac tacagtgatg tcaagaaaat gacgtcaaaa gagttgaagc aatccatcaa
    24001 agcgagtgaa ggacgggtga ttgtttgtga aacggttgct aatactcttc cacaatctgg
    24061 agaaattacg aatgcagaag ttgcgtcagc atttggtgca gatttgattc ttttaaatgg
    24121 tttcgattgt tatcgtcctg cagtttttgg actaccaaat gtgacgattg agaaggtttt
    24181 tgatctacag caaacgattg aaaacccgat tcctaaatta aaagaactag tcggtcgtcc
    24241 tgtgggaatt aatttagaac ccattgcccc taaaacaaat atgatgagtg agaaagtaga
    24301 aatcagccaa ggaagagcaa gctctgttga aacgatacaa aaagcagaag aactaggagt
    24361 cgattttatt tgctttaccg gtaatccagg cactggagta accaatttag aaattactaa
    24421 atccgtaaaa atggcaaaaa agtatttttc gggattgatt attgctggga aaatgcatag
    24481 cgccggctca gaagagccag ttgtgaataa agaggcgatt atttcttatg cggaagcagg
    24541 cgcagatatt ttactagttc cttcagtagg gtctatccca ggctttttag agcaagacct
    24601 aattgaagcg gttcggttgg caaaaaaata tgatttgtta acgatgtctg caatcggtac
    24661 tagtcaggaa agtgcccgac cagaaacatt gcggcaatta gccattatga ataaaagttg
    24721 tggtgttgat ttacaacata ttggagatgc tggatacggg ggattggcac cagcagaaaa
    24781 tattttcgaa acctcggttg caattagagg aatgcgacat accatcaact gcgttagccg
    24841 ctcgatcaat cgctaacaaa attaaataaa aaatggttaa attgattatt taagtgggaa
    24901 catacttgag atatcagcta acttctgtca ctttaaaaaa gggactgtga cagaagtgaa
    24961 aatagacaaa aaatatccga acaagagact aggatagcag aaaaactatc gttttgcaac
    25021 gaggaccaac tggaccgtag gagcaattct cttattgttc ggatatttcg cttgttcaag
    25081 gacttatgtc tgtagtgccc ttttttggta ggaaaaatgg tagggaacca tatcattttt
    25141 aatgaaaatc aagcatttca aaagttgaaa aacgttgatt tatcggcatt ccccatccat
    25201 attatattta cgtcttattc ccactccact gttgcaggtg gtttgctggt aatatcatac
    25261 acgatacggt taacgtgtgc gacttcattt acgatacgga cactgatttt ttgcaagact
    25321 tcccaaggga tacgggcaaa gtcagcggtc atgccgtcaa tagaagtgac agcacggata
    25381 cctacagtgt agtcatacgt acggccatct cccataaccc caacagagcg aatgcctggt
    25441 aggacggtga agtattgcca gatgtcgcga tctaaaccgg cattggcgat ttcttcgcgc
    25501 aagatagcgt ctgattcacg gacgatttct aatttttctt ctgtcaattc accaaggaca
    25561 cgaataccta agcctggtcc agggaatggt tggcgccaaa cgatgctgtc aggcatgcct
    25621 aattgggtac ctaattcacg tacttcgtct ttgaacaatg tgttcaatgg ctcgatcagc
    25681 ttgaattgca tgtcttcagg taatccacct acattgtgat gtgatttgat ggtttgggca
    25741 gtttcagttc ctgattcgat cacatcggta taaagggttc cttgtgcaag gaaagaaacg
    25801 ccttcttcac cggcaagttt ggttgcttca tcatcgaaga catatacaaa ttcatttcca
    25861 atgattttac gtttttcttc tggatcagac acgccagcaa gttttgataa gaaacgttct
    25921 ttggcatcga cttttataat gttcaagcca aattttccgc caagactttc cataacttgt
    25981 tctgcttcac ctttacgcaa caatacgtga tcaacgaaga tacatgttaa ttgatcgccg
    26041 atcgcttttt gtaaaagaac accaacaaca gaggaatcta ctccgcctga aaggcctaat
    26101 aaaacttttt tgtcaccgac ttgttcacga atttttgcga tctccatgtc gatgaagtta
    26161 tccattgtcc agtcaccagt acattggcag acatctagtg taaaatggcg caacaagtca
    26221 tttccatatt cggaatggcg aacttctgca tggaattgga ttccgtacat tttgcgttct
    26281 gcattttgga tcgatgcgat ggggcaatct ttgctggtag caacgacatc gaaatcagtc
    26341 ggtgcttggg taactaggtc accatgactc atccaaacgg tttgtttttc aggagtgttt
    26401 tggaaaaggg ccgcttctgc agaagtgatt tccaattctg cttttccgta ttcacggttt
    26461 ttggctggtt taacttttcc gcctaattta taagtaatca actgcatacc gtaacagatt
    26521 cctaaaactg ggatccccaa atcaaaaatc ttttcatcaa taccaaaggc cttctcatca
    26581 taaacactgt ttggtccgcc agaaaggatg attcctttag ggttcattgc ctttacttct
    26641 tctgcggtaa tgcggtggct catcaactct gagaaaacac cgaactcacg aatacggcga
    26701 gtgatcagtt ggttatactg actaccgtaa tcaagtacga taatttttgg ctcttcgtca
    26761 aatcgtgttg gtgaagacaa atttgaataa aacttagtcc gatatgcggc gcgttctgtg
    26821 cgccgcatat ttttttagtt ttcaaagatc agaccttgtt gtaaatagat ccgtgcccga
    26881 aagttataaa agttgcgata accaaacgca attcgtttaa tcagcttgat cttgttattc
    26941 agcccctcag tgacgccgtt tgagtgggac accttaaagg catttgaaat acccgattgg
    27001 tagcgatgga agatcgtgaa tttcttttta aacgactgag gtaacgtaga tgaaattgtt
    27061 ttaagggttt cttgaaagcc ttccgtacct cgtttaacga aataatagcg taataattga
    27121 caaaaatgat acgcagttcg caattcttcg ttgtagctta acagctcatc aacgatttct
    27181 gtttgactaa ttggtcgttt aaacagtcga tgataatgcc gtttgagtgg ctccaatgtc
    27241 gtggaatctt tgagtaagag tttccaatat cgcttgagcc gacggtactt cttctgattt
    27301 tcagagtgtg aggttcgaaa acgattcatg atctggacac gtaattgatt gaagctgcga
    27361 ttgatgtgtt ggacaatatg aaagcggtca gttacgatct ccgcatttgg aaagactgtt
    27421 ttaagcaact gacaatagct ggcattcatg tccatcacta ggtacttgac ctttaaacgg
    27481 gcttttctag gaaagcgcag gaagtattga gttagtttgt aaagtctgcg atcttcaagg
    27541 atcgtaaata gctgatgggt tgtcccatca acacaaataa aactcatgga accagaaaca
    27601 gacttcattg atttgaactc atccatacac aacaccgtcg gaaggaagtt catacgtggc
    27661 tggtaatgct tcgttgcttc acgcaagatc cgtgtcaccg tcccatccga gacgaagtac
    27721 ttcttagcga ttagtttacg tgattgattt tcgtacagtt ctatgagaat cgcctgcttc
    27781 agttcctttg ttagttgatg atgttcatca actaagtccg ttttcgcaga aaaggtctgc
    27841 ccacaatcat agcatagaaa acgggtacgt tttaattcta aaatagtcaa cttttctttg
    27901 actttcaata tctgagtacg tgtcgtatag gaaccgttgc ggataatacg aatcgatcca
    27961 cagtgttcac aatgggaagg tgagtcatcc aatctccctt taataaactg cgcttgaacg
    28021 cctttatatt ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat
    28081 ccgagtaatt ttctagtatg attatccata gagcgactcc tttggtaggg tgatgttttg
    28141 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta
    28201 gtagaaaatc gccttcgatt ttccaacaac acaaaatatt atagaaccct ttattctaaa
    28261 ggatttcaag gtgtctgttt gcatttatta gaaaaaagaa aaagtattgg caacagaagg
    28321 tgtcaccaac actagatatt atagagccat aatatgctgt ataatatcta gtgtataggc
    28381 ttaagtggag gtatatatga aaatattaac tgttgaagat gataatatga taagagaggg
    28441 aataagtgaa tatctttcag aatttggata tactgttatt caagctaaag atggaagaga
    28501 agccttgtca aaatttaata gcgatataaa tttggttatc ttagacattc agataccttt
    28561 tataaatggt ttagaagtgt tgaaagaaat tagaaagaaa agcaatttac caattctaat
    28621 cttgactgca tttagtgatg aagaatataa aattgatgca tttactaatt tagtagacgg
    28681 atatgtagaa aagccatttt cattgccggt cttaaaagct agaatagatt ctttaattaa
    28741 gaagaattat ggacatttag agaagtttga atataaggat ctatcggtta attttaatag
    28801 ctatacagct aaaataaatg gtgaagaaat agatgtaaat gcaaaagaac tagaagtatt
    28861 aaaatgttta ttggataata atggacaagt gttaacaaga atgcaaatta ttgattatgt
    28921 atggaaagat agcgaagaaa ctccttatga tcgagtaata gatgtatata taaaagaact
    28981 tagaaaaaaa ttacaattag actgtataac tactataaga aatgtaggat ataaattgga
    29041 gagaaaatga aaaaattaaa gatatttcca aaaatgttca tacaaatatt ttctgttctt
    29101 ggaataataa ttatattagt tcattcatta atttttttta tctttcctaa aacatatttg
    29161 gagacgagaa aagaaaagat tcataatatg gcaaatgaaa tttctagtaa tatgaatgga
    29221 aaagaaataa aatatataga acaaactttg gagttttatt ccaagagtag tgaaataaaa
    29281 gtattcataa aagaaaaaaa taataagaat gaaatacaaa tcaaagataa tataaatgtt
    29341 aatttagaaa gtgatagtaa tagtcaagtc cagactcctg tgtaaaatgc tatacaatgt
    29401 ttttaccatt tctacttatc aaaattgatg tattttcttg aagaataaat ccattcatca
    29461 tgtaggtcca taagaacggc tccaattaag cgattggctg atgtttgatt ggggaagatg
    29521 cgaataatct tttctcttct gcgtacttct tgattcagtc gttcaattag attggtactc
    29581 tttagtcgat tgtgggaatt tccttgtacg gtatattgaa aggcgtcttc gaatccatca
    29641 tccaatgatg cgcaagcttt tgaatatttt ggttgatcga tataatcatg aatcaatcga
    29701 tttttagcct cacgcgctaa gttaatatct gtgaacttaa aaattccttt aacagcttct
    29761 ctgaaagatt ttgaattttt tttaggaatg gtggtaaaga tatttcttag gaagtgaact
    29821 tggcatcttt gccaacttac gttggtgaag gattttctaa tggcagagac taatcctttg
    29881 tgcgcatcag aaataacgag ttccgtacct tgtaaaccgc gttcttttag gtattcaaaa
    29941 aatgttgtcc aggtctcttc gctttcgcca ctttgaatca tgaagccgat aatttcacgg
    30001 tcgccatctt tggttattcc aatcgctata tgacagcttt ttgagagtac tcgattttct
    30061 tctcgtactt ttatatagag tacatcggtc attaagtaag gataattttt ttctgataat
    30121 aaacgattct gccactcgtt aaccataggt tctagctgtt ctgttaagct agaaacgaag
    30181 gacttagaga cggatttacc acaaagttct tccacaattt ttgatacttt acgagttgaa
    30241 acgcctgata catacatttc caacattgaa gccatgaggg ctttttcgtt tcgttgataa
    30301 cgttcaaaca ctgtgggtga aaaatggcca tcacgtgttc tgggtacttt taattctagc
    30361 gtgcctacac gtgtcgtaaa gctgcgctca taatagccat ttcgttgact ttgtcggttt
    30421 tctgttcgtt catattcttt tgcttgaata tattctgttc gttgattttc cattagttga
    30481 ttaaataccg ttgttaaaat atttttagaa acgtcatcct ttacagaata ttcaataatg
    30541 ctttgaatct cttcgctttt cagtgtaaaa tgtacttggg tcatgtaaaa gtcctcctgg
    30601 gtatgttttt gtcgttaaaa acattgtacc gtaaaaggac tgttatatgg cctttttact
    30661 tttacacaat tatacggact ttatctaaat aatgatagat acataggcat agccaaaact
    30721 aaggcttgca gtatagttac tccattatct atagacaaaa tactgattga tagtaagcgc
    30781 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    30841 tctggaggt
//
LOCUS       DACDLI010000028        30528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDLI010000028 DACDLI010000000
VERSION     DACDLI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.028"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            289..696
                     /locus_tag="IV353_001308"
     CDS             289..696
                     /locus_tag="IV353_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009568.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="IV353_001309"
     CDS             710..1111
                     /locus_tag="IV353_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009569.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="IV353_001310"
     CDS             1113..1484
                     /locus_tag="IV353_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009570.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1520..1822)
                     /locus_tag="IV353_001311"
     CDS             complement(1520..1822)
                     /locus_tag="IV353_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009571.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2071..2271)
                     /locus_tag="IV353_001312"
     CDS             complement(2071..2271)
                     /locus_tag="IV353_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9009572.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2576..3808)
                     /locus_tag="IV353_001313"
     CDS             complement(2576..3808)
                     /locus_tag="IV353_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9009573.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4062..4631
                     /locus_tag="IV353_001314"
     CDS             4062..4631
                     /locus_tag="IV353_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9009574.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4809..5249)
                     /locus_tag="IV353_001315"
     CDS             complement(4809..5249)
                     /locus_tag="IV353_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9009575.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5407..6171
                     /gene="map"
                     /locus_tag="IV353_001316"
     CDS             5407..6171
                     /gene="map"
                     /locus_tag="IV353_001316"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9009576.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6203..7126
                     /locus_tag="IV353_001317"
     CDS             6203..7126
                     /locus_tag="IV353_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9009577.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7202..8341
                     /locus_tag="IV353_001318"
     CDS             7202..8341
                     /locus_tag="IV353_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9009578.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8334..9134
                     /locus_tag="IV353_001319"
     CDS             8334..9134
                     /locus_tag="IV353_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9009579.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9134..9961
                     /locus_tag="IV353_001320"
     CDS             9134..9961
                     /locus_tag="IV353_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9009580.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9939..10673
                     /locus_tag="IV353_001321"
     CDS             9939..10673
                     /locus_tag="IV353_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9009581.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10773..11639
                     /gene="rfbA"
                     /locus_tag="IV353_001322"
     CDS             10773..11639
                     /gene="rfbA"
                     /locus_tag="IV353_001322"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9009582.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11653..12225
                     /gene="rfbC"
                     /locus_tag="IV353_001323"
     CDS             11653..12225
                     /gene="rfbC"
                     /locus_tag="IV353_001323"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9009583.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12247..13275
                     /gene="rfbB"
                     /locus_tag="IV353_001324"
     CDS             12247..13275
                     /gene="rfbB"
                     /locus_tag="IV353_001324"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9009584.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13373..14224
                     /gene="rfbD"
                     /locus_tag="IV353_001325"
     CDS             13373..14224
                     /gene="rfbD"
                     /locus_tag="IV353_001325"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9009585.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14259..16292
                     /locus_tag="IV353_001326"
     CDS             14259..16292
                     /locus_tag="IV353_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009586.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16336..17616
                     /locus_tag="IV353_001327"
     CDS             16336..17616
                     /locus_tag="IV353_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9009587.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGICVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17826..18632
                     /locus_tag="IV353_001328"
     CDS             17826..18632
                     /locus_tag="IV353_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009588.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18644..19864
                     /locus_tag="IV353_001329"
     CDS             18644..19864
                     /locus_tag="IV353_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009589.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19854..21440
                     /locus_tag="IV353_001330"
     CDS             19854..21440
                     /locus_tag="IV353_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9009590.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21479..23617
                     /locus_tag="IV353_001331"
     CDS             21479..23617
                     /locus_tag="IV353_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9009591.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(23985..24968)
                     /locus_tag="IV353_001332"
     CDS             complement(23985..24968)
                     /locus_tag="IV353_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9009592.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            25346..26737
                     /locus_tag="IV353_001333"
     CDS             25346..26737
                     /locus_tag="IV353_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9009593.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            26753..27793
                     /locus_tag="IV353_001334"
     CDS             26753..27793
                     /locus_tag="IV353_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9009594.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            27812..28897
                     /locus_tag="IV353_001335"
     CDS             27812..28897
                     /locus_tag="IV353_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9009595.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            28930..29892
                     /locus_tag="IV353_001336"
     CDS             28930..29892
                     /locus_tag="IV353_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9009596.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            29885..>30528
                     /locus_tag="IV353_001337"
     CDS             29885..>30528
                     /locus_tag="IV353_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HAP9009597.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNF"
BASE COUNT         9964 a         4933 c         6035 g         9596 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctttgt atctttctgg atcaacgaaa gtatacttta
     1561 tatagtcata acgccgatga tcgctacgtg cgtccggcac gtcagtcacg atatcaaaca
     1621 aaaagtatac acccttcttc attctagttt tcgcagcagg aattttgaaa tagttcttat
     1681 tagaatagta gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat
     1741 tccatacttt ataaaaatct ttgataaatc tattcgaagg gtcaaattta aaccataatt
     1801 gtgtttttcc ttccatcagc ataaaagttc acctcaaaaa gagtatacga acgaatgttc
     1861 tttttgtgaa ggcggaattt aatatactga ataatagaaa acaagtactt gtgccaaatt
     1921 atgtgccaaa aaaattcgaa ttcaacaaaa tgtaaacaaa tagaaaacct tctatattaa
     1981 gatttcctgc aataacaaac acaacagaag acgtgtaata gttagtcagg aacgtacaaa
     2041 taacccctgt atcctttgcg gtacaggggg ttattttgta taagtagaca ttgtagtttc
     2101 taaactgcta tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa
     2161 aatagatgca tgcgtatgtc ttagtccatc aataaacagt cgaagagaaa atagggaaaa
     2221 attgattact aaagaaaaac ttactaggtc gacggtgtag ttgattttca tacttcaata
     2281 gattcgaatt aaacgatttc gattggctta taccagtcct atccaacgaa agatatatcg
     2341 ttcttaactc tagcggtata acttgaagct ttacgtaaag ccaatgggaa tgaactggag
     2401 atgacttttt attattttta gtaatagctg atgggttaat acttacaact ttccttttca
     2461 aagtaccata taaaaaaacg gtggggttgt ttagcgaaat aaaggatcta agccaatagc
     2521 ttagatcctt tattctatgc tttatattat tttttgcatg tgtgcgaaag tatactcaag
     2581 cccaatcgcc atttcggaaa atcggtactg tagagccatc ttcacggatt ccgtcgatat
     2641 ccatcttgtc agatcctacc atgaaatcta cgtgggtttg acttctattc aatccagctt
     2701 ctgctaattc ttcatcggac atttctgtac cgcctttcac actgaaggca taagcagaac
     2761 ctagtgctaa gtgattcgat gcgttttcat caaataatgt attgaaaaag atgatgccag
     2821 attgagaaat aggtgaaggg tcaggtacaa gtgcgacttc tcctaaacga cgcgcacctt
     2881 catctgtatc aagcagtttt gctaagacat cttctccttg ttcggcagaa aaatcaacaa
     2941 cttttccatc tttaaaagta aatttcatac ccgagatgat ggtgccagca taactcagtg
     3001 gctttgtact tgaaatgtaa ccatctactc gatgactgtc aggtgctgtg aacacttctt
     3061 ccgtcggcat atttgccatg aatttttctc cacgagcatt atagcttcca gcgccttccc
     3121 aaagatggtt tttaggcaat ccgataatga tatctgtacc gggagcagta tagtgaaggg
     3181 cagaaaattg ttctcgattc aattcttctg ctttttttgc taatttttca tcatgcttct
     3241 tccatgctaa gacagggtct tcttcataga cacgagtcgt tttgaaaatc tgatcccata
     3301 atgcctcaac ttgtttttct tctggcagtt ctgggaagac ttttgctgcc cattgtttac
     3361 cagccgcagc cacgacagtc caactgactt tatttgcttg agttgccttg cgtaaattca
     3421 ttagtgcttt gccattcgct gattgataag acgctactcg atggctatct acaccagcaa
     3481 atgcatcggg atcggcagaa acaacgctga tcctgcttgc tcctttttca agccattcat
     3541 ccgcttgatc gattttagat tgtggtacat tctcaatacg atccgttgca gcatgtaaaa
     3601 gaaattctct ttggatctgg tcgtcagtcc attgaacgat aacctcagcg gctcctagtt
     3661 tataagcttc ttgtgtgatc aatcgagcaa ggggtgcctg ttccacgctg atctgtaaaa
     3721 caactgtgtg tcctttttct gtggctacgc cagtttctgc aattaagcgt gcatattttt
     3781 ttagaagttc attaaaatca gataacataa taaaaattcc tcctcaccat tttttctggt
     3841 aactattagc atgataccat ttaattatga ttcaaacaat gaggctttaa ataaaaaaag
     3901 gaggttattt ttttaaggtt aagtttcatg atatatgtta ctacttgcta tttcagcttg
     3961 tgaagaaaat caaaaactaa ttttgcatat caaaaaaaca tgttacaatg aaattaatca
     4021 atggtggaag cgttataaaa aaattaagga gattaaaaaa tatggcaaga aaaaagacga
     4081 ttactagaga acagattttg gcagctgctt atgaagtagc tgccaccgat ggatttacac
     4141 gatttactgc gagaaacatc gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg
     4201 agtttaaaaa tatggatgac ttgagacaag ctctttttga gcagatctat gatcatttac
     4261 aatatgaagt gttcactgtt gagcatacag ggaatacgat tatcgatctt gcttttaatt
     4321 atatccattt tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg
     4381 gtggaggaaa agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg
     4441 atccagctta tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga
     4501 tcatagctac tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac
     4561 agattgaaaa gatgatccaa gacagtatag atgctatctt gaaattcaaa gaaccaatcg
     4621 aaatcgattg aatatactca aaagttccac aaatcagtag gtaacaagag atgtgtcaaa
     4681 ataaggaaaa gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc
     4741 atgaatgcaa tgaataaaag agccatacag attgaataaa tctggatggc tcttttattg
     4801 caagtatatt aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct
     4861 tcttcttcag cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt
     4921 gcaaaagcag catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca
     4981 gatccgcaga caccgtaaat ttttcccaat agatccaatt cttgcaagtc ttcataaaag
     5041 tctacgatct catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata
     5101 tctgcctcct cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata
     5161 tccagatttt caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg
     5221 ctagcatata cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca
     5281 attatgaatt ataccaaaac atcattgaaa agaaaataaa acctaaagac acctggggaa
     5341 tgtcatttgg ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac
     5401 gaaaatatga ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct
     5461 ttattagcag atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg
     5521 gatattgagg tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt
     5581 ggctttgaag ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga
     5641 ttccctcgta aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat
     5701 ttgaaaggcg gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag
     5761 atcgaccgat tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa
     5821 gtagggaatc ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat
     5881 ctagcgatcg ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca
     5941 gcagttcctc attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc
     6001 acgatcgaac ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg
     6061 actgcgtata caagagacgg cggactttct tgccaatatg aacatacatt ggctattact
     6121 aaagatggtc caagaatttt gacatcacaa ggagaagaag gcacttattg atctatccag
     6181 tcaataggta cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct
     6241 gatgcgcttt attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc
     6301 tgtcgtcgtt gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa
     6361 tattttaccg tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat
     6421 tcctgagacc gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc
     6481 aggaggcttg ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa
     6541 tgctttgcaa attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat
     6601 cgtccgtttc ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac
     6661 tatcgttctt gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc
     6721 cgtgaacttt atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt
     6781 tgtcatcatg ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat
     6841 cattccgggc acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat
     6901 ctatgctagg tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt
     6961 actcatgctt tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc
     7021 agttgttcaa gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa
     7081 atggttcaat aaattgaaaa ctaaattttc tcataaaaaa aagtagaaac aagagttctt
     7141 attgtaaatt tatatcagta atttataata attatgactc ttaattaaag taggtgccca
     7201 tatgcttgtt tcttttagta tggttataag attttttctg acaattttat tatcactgat
     7261 attaacacct gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga
     7321 aagaagaata aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt
     7381 tgttatatcg actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat
     7441 cttgattgct tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac
     7501 acctaaacaa aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga
     7561 tatacgtatt gattccttca cgcttccttt tattggatat atccaactag gctggcttag
     7621 ttttcccgta acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg
     7681 tttggatggg ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg
     7741 atattttttc ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc
     7801 aagtattatc ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac
     7861 aggagctcta ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt
     7921 gacttttgtt tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt
     7981 ttttgctatc attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca
     8041 tcttcatcac cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta
     8101 tgcgatggct ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg
     8161 aacgatcata ttgattattg ccatgttgtt tgctgcaatt ttattatttg aattgattgg
     8221 tctgatcaat gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga
     8281 gtaccgtacg caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata
     8341 acaaattaaa ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc
     8401 ggacactaga taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt
     8461 ttgacaacaa ttcacttccc gagtacaaaa aaagactaac agagtatagg aaatatccct
     8521 acatcgatat ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc
     8581 tgatagctag cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag
     8641 agacagttac agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac
     8701 ctaagattct taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt
     8761 ttgattacgt tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac
     8821 tggctgtgtt tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct
     8881 caggtagttt tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc
     8941 gtttcttcat gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca
     9001 agttattgta cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa
     9061 atgcaaaact gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt
     9121 ggcgtttttt ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt
     9181 cagtcaatct ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct
     9241 tattatttac gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat
     9301 cgtttatcat catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc
     9361 tcaagcagat gaaggaacac gttgtctcgt cactctggat caggatacga ggctagtcga
     9421 cgattatttt gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc
     9481 gatgattttt tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg
     9541 tacagctcaa gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc
     9601 aggtgttgcc tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc
     9661 tttagatttt ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga
     9721 gatccttcct gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag
     9781 taatcgatac cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag
     9841 gcatttatcc agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt
     9901 caaaaaccgg catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg
     9961 aattcgtgcg ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt
    10021 tagatagtat tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt
    10081 cagtagatga tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc
    10141 ttttttcggg gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga
    10201 aaggagatct aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa
    10261 agatcactga ctattttgag cagaatccgg atcagctagt agtagtttct gatttaatca
    10321 ttgtggacag cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag
    10381 aaggatggct taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt
    10441 cttctttaaa acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga
    10501 ttggtttgct gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa
    10561 gacatgattt aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt
    10621 ggcgtgttca tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga
    10681 tgtttaaatc cttaatattt ccatgacaaa tataacaatt gatgagataa taacaaagtg
    10741 aaatttgaaa tcaaaaagga gagcagatat ccatgaaggg aatcattctt gctgggggca
    10801 gcggaacacg tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg
    10861 acaaaccaat gatttattat ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc
    10921 tgattatttc aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg
    10981 atttaggact gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt
    11041 ttatcattgg agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata
    11101 tttactatgg tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg
    11161 cgacagtatt tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg
    11221 agaatatgca agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg
    11281 taactggcct ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt
    11341 cagaacgtgg agaactagag attacggatg tcaataaagc atatttggat aaaggaaaac
    11401 tatccgtaga acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat
    11461 tgcttgaagc atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt
    11521 gtttggaaga aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg
    11581 cacagccatt gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag
    11641 gagtccttac agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg
    11701 gacgtctttg gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca
    11761 gaacatggat tgaaatatga ctttgttcaa gacaaccatt cactatcagc tgaagctggt
    11821 gtccttcggg ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta
    11881 gtcactggag cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt
    11941 caatgggacg gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga
    12001 tatgcccatg gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat
    12061 tactataatg cagaagcaga tgggggcatc gcatttgatg atccagattt agcgattgat
    12121 tggccaattg atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta
    12181 aaagaatttg aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat
    12241 tttcttatga aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt
    12301 cattacgtgg taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat
    12361 gcaggaaaca aagagaactt ggccggactt cctagcgatc gcgtagaatt ggtcgtagga
    12421 gatgttgcag atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat
    12481 tacgcggcag aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg
    12541 aatatcattg gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat
    12601 cacgtatcta ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat
    12661 ggggaaggcg aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat
    12721 tcttctacaa aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta
    12781 cgtgcaacca tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt
    12841 attcctcgcc aagtaacgag tatcttaagc ggtattcgtc caaaattata tggcgaagga
    12901 aagaatgttc gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg
    12961 acgaaaggca gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag
    13021 accgtgatgg aattgatctt agaaatgatg ggccaaccaa aagacgcata cgaccatgta
    13081 aacgaccgcg cagggcacga tttgcgttat gcaatcgatt cgacaaaatt gcgagaagag
    13141 ctaggttggc agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg
    13201 tacgaagaac atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc
    13261 aaaaatggac aataatacag aggaaatttc actgtattaa tggaaaaaca catgtgaggc
    13321 aggcgctcgc ctgtctcttt tttaaaataa aaatcaagga gcggataacc atatgttttt
    13381 attaactgga ggaaatggac aattaggtac agaattgcgc catttattag atgaaaaaaa
    13441 tctgccatat gtttcaacag atgcaaaaga cttggatatc acagatgctg agaaaacaat
    13501 ggcttatatt acagaattaa aacccgatgt tattttccat tgcgcagctt acacagcagt
    13561 agataaagca gaagaagaag caaaagaatt agatgagaaa atcaatgtcg atggcacaag
    13621 aaatgtagca cttgcagcaa aagctgtgaa tgcacaattc gtctatatca gtacagacta
    13681 tgttttcgat ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact
    13741 aaatgaatat ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga
    13801 ctattatatc atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac
    13861 gatgcaacgc ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg
    13921 accaacttgg acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc
    13981 atttggtatt tatcatttgt cgaacgaaaa tagctgttct tggtatgagt tcgcaaaaga
    14041 aatattgaaa gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa
    14101 agcaacacgt ccgcagtatt cagtcatgag cctgaaaaag acagaagcgt taggttttgt
    14161 cataccaact tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa
    14221 gtaatttcct ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg
    14281 cacgacgaaa agaaaagcca aaagcgaatc aggcagattc gaatttggtg cttgacctgt
    14341 ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt
    14401 taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa
    14461 aaactgatgt gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga
    14521 caccttttta cctctcacaa gctgaagagg acttttcgtt ggtgaacgag gattattcat
    14581 taaacggaca aaacatgatc attgcgtata attctcctgt aaaggatatt acggttatcg
    14641 gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt
    14701 gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga
    14761 agaggaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc
    14821 agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgattg
    14881 ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt
    14941 tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc
    15001 tggcgtactt gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt
    15061 catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca
    15121 tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc
    15181 caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta
    15241 caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt
    15301 tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag
    15361 atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt
    15421 ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc
    15481 cattaagagc catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg
    15541 cttatatttg gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa
    15601 atgcatggtc ttatttctat gcgattacgc attcgaaaat ggataattat ttttcggatt
    15661 ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcttttgca ttatttggct
    15721 ggagacgctt gttttacctc gcttttgtcg ccttagttat cgtatctggc tttttcgtca
    15781 atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa
    15841 aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca
    15901 ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat
    15961 ggaagaaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa
    16021 gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg
    16081 tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac
    16141 cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata
    16201 aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac
    16261 aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata
    16321 ttagtagaaa ggagagtgaa gataattcaa gcaatcgtac aaaaatttgt caacaaaatg
    16381 agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcaggggta
    16441 ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg
    16501 gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt
    16561 gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta
    16621 tttggagact tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag
    16681 tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg
    16741 ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
    16801 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
    16861 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgttcg aatcatcggt
    16921 gggatagcag tcgttgcagc ctttttaggt atttgtgtct gccaatctag aggtgcatta
    16981 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
    17041 cgtgcgccat ttattgccta cacggttacg attcttttag ctttgccgat ttcttatctg
    17101 gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc
    17161 tatcagacgc taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc
    17221 cagcgaggaa accaattttt aggaaaccac aatagttata attctatttt gaatatctat
    17281 ggattgatag gatttgggat tgctgctttg ctgcttatcc tttttattgg tcgcttaacg
    17341 ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg
    17401 atgcagtcat ttatggagga tacattgact tcatacccat gggttccgat tatttatatc
    17461 ctgttggcta tggccgttca tcgatatgat gaaccagaag ttacggagga attctatgtt
    17521 atggaagagg catatgacga tatgacttat gatgatacat cttatgagga acaaccagat
    17581 agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaatac gagagaggct
    17641 gtgacaaccg tcgcagcctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata
    17701 gaaacgttag agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg
    17761 gtaccaaaaa tatggtaaaa tggcacttga ctgtaattca ctgaaaatcg cttggaggaa
    17821 cacaaatgtt taaagatttg tatttattta tgaaggacat ttatcagaac agaaaattac
    17881 tgctacagtt ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct
    17941 tatgggcgtt tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg
    18001 gactgaaagc acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga
    18061 tgattccctg gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat
    18121 atacttattt agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc
    18181 tatccaatct gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa
    18241 atggtatata tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg
    18301 ctttcttgac aggattgact tggctgacgg catcgatcca accattttta cctgatatca
    18361 tgcaattaat tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc
    18421 ctaatcagtt tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg
    18481 ttcaaggtta tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata
    18541 ctttgtattt ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta
    18601 gattgaaacc acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat
    18661 tagactaaaa gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg
    18721 tgaagccctc aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt
    18781 gaatgtagaa ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa
    18841 atctacaatg ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat
    18901 cgaaggaaaa atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg
    18961 ctatgaaaat atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga
    19021 aatggtcgat gatatcattg aatttgctga catcggcgac catatttatc aacctgtaaa
    19081 aacctattca agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc
    19141 tgatattttg attgtggatg aagcattggc agtaggagat cttgaatttc aactaaaatg
    19201 tatggaaaaa tttacagaaa tcaagaactc gggaaagacg attttattcg tttctcatga
    19261 tattagttcg attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gaaaagttgt
    19321 cgaatatggt gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc
    19381 agttaaaaca gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt
    19441 agacgtgacg aaagccgaat tgttggataa agataaaaat ccagtggata tgctggaaca
    19501 aggagaaaaa ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc
    19561 agttttagga attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct
    19621 actagatggt atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga
    19681 aaagttaggt ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc
    19741 aacggttcct ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat
    19801 cggagaaggc atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat
    19861 atgattttga aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca
    19921 aaccaggaag taaagtttta gagtttggtc ctggaaacgg ccgattaacg aaacatttga
    19981 tcggagcaaa acaatgtgaa gtgagcattg tcgagctaga taaggaattg ttcgattttg
    20041 tcagcgagtt tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact
    20101 attatgctgg acaaacattt gattacgttt tgtttgccga tgttttagaa catttggtag
    20161 atcctggaaa aacgttgaag aaagttagag aatttttgaa tgaagaagga gaaattctaa
    20221 ttacattccc taaccttgcg cataattctg tcatgattga tttgttcaat aatcaattgc
    20281 catgggcttc ttatggtttg ctagatgaaa cccataattc tttttatact catgatggct
    20341 tccaaaaagt ttttgaaaaa gcaggattat acatcaatat tgaagattat ttgtacttag
    20401 ctgtaggtga tacagaatta aaatcaactt atgaagaatt gccggaagct gtacgttatg
    20461 atttcaaaat gcgaccattt ggcgaagtct accaatactt cttctcattg atgaaacatc
    20521 cagtagcaca aagttccatt gcgcagccgc aaaattctaa ttatgtgaaa gtattagaag
    20581 taatccagca aacgaaacaa gatgaaataa ttcaacaaat accattcaat aatttcacag
    20641 gggaaaatga gacactaagt ttccatgtat cagaattagc agaacgaatg gtgtttcgct
    20701 tcgcgcagca acctagcttt atcgaattca gcgcagaagc tgctggagaa aaactcacat
    20761 ttatcgattc aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatggtaaag
    20821 atttaccaga gttcttttta acggatattg cagggaaaga agtgactatc cattgtcact
    20881 atcgttttat cggagaattg acaccaacaa tgaaagaatt gctggaaaca atccgtccaa
    20941 tggctgaagt tacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcatt
    21001 atcttattga gaataataca agattagatc agacgttgaa aacaaccata aatcgctatt
    21061 gcacgttgtt ggaaagtgat gaatggacaa tcaaacatag attagggcga cgtaagaaag
    21121 aaacatcgaa aaaaatccaa gaaaaagagc tttctctatg tattgacgaa aaaatctggg
    21181 atgcagaaac aaagatattg aaaatcatcg ggtggggaat cgctaattcg gatcgccaac
    21241 cattgtccta taagttatca gccgaccaat caccattttt tgaagcaatt cctgttttac
    21301 gagaagaagt caatcaagca aaacagctag ctgaaggagc aaaagcagga tttgaactgc
    21361 ggatcctttg cgagaaggaa cgttcattct tagttgaagc agtggctcaa aatggtcaat
    21421 catggatcat tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt
    21481 gcaaaaaaaa gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga
    21541 tttgcttatt gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt
    21601 agaaaaagaa aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt
    21661 atataatctt gatgttaaga cccaaagcgg ttttaaggta cgtttatcag ggaaaatgag
    21721 aggaaaagca atcttagact tccaaacagc aagacatcaa aatggcatag ctgtgaaatt
    21781 aaacggtaaa tatccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt
    21841 actgaaaaaa gggataaatt atgcaaggac tcatggcgta aaaaaagcaa tccgccgggt
    21901 caaactggaa ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcatgagaa
    21961 acctgatctg aaagagcaaa ggaaagaagt acaaggattt gcctaccgcc cattgatttc
    22021 catacttatg cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt
    22081 acttgatcaa acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc
    22141 agctatccga aagtgtctag aatcctatca agcaaaagat gatagaatta aagtcgtttt
    22201 ccgacaagaa aatggacaca tcagtctggc aactaattct gcattggaaa tggcagaagg
    22261 agagtttata gcgctcttag ataatgatga cgaattacct ccatttgcct tatatgaagt
    22321 ggcaaaagtg ctaaacgtac atccagaact ggatttgatc tacagtgacg aagataaaat
    22381 cgatgcagat gggaatcgct ttgacccgca cttcaaagca gactggtctc ccgatacatt
    22441 gatgggaaat aattatatct ctcacttagg cgtatatcgg acaagcattg ttaaggaact
    22501 tggcggtttc cgtaaaggat atgaaggctc tcaagattat gatttggttc ttcgtgtgac
    22561 agaacaaatt cctgcagaac acatttacca cattgaccgt gtcttgtatc attggcggac
    22621 gatcccggga tcaactgcaa gcaatggaga agctaaaagc tacatctatg attctggagt
    22681 gaaagcattg acagatgctc tatctagaag aaatatcaag ggaagcgtac atcctggcag
    22741 aatttctggt ttttatgaaa tcacttatga tgttttgcaa gaagacttgg tcagtgtgat
    22801 cattccaact aagaatggct atgaagatct aaaaacttgt gtggattcga tcatcgaaaa
    22861 aacgtcttat ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat
    22921 gcaagaatta tttgcagaat acaagcatca gctgaaagac cgatttatcg tcgaattaat
    22981 tgacattcca ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa
    23041 atacttttta ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat
    23101 ggtttcttat gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga
    23161 tgatacaacg cagcatgcag gagtattatt aggaatcggt ggtgttgcag ggcatgcatt
    23221 gaataactat gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta
    23281 cttagcagtc actgcagctt gtatgatggt caaggcagct gacttcaatg ctgttaacgg
    23341 gtttgatgaa acattagaag tagcattcaa cgatgttgac ttgtgcttga aagtttatga
    23401 actgggtcgt tacaatgtct atgcacatca agcagaattg taccatttcg aatctaaatc
    23461 aagaggttat gaagatactc cagaaaaaca aagacgtttt gctggtgaaa tcaaaaaaat
    23521 gcaagataaa tggccagcat atattgcgca tgatccattt tacaatgata atttaacaaa
    23581 agaaggaatc ggcgactttt cgttgagacc agattaaact ataataaaaa gctgttatgt
    23641 tgtgattcgc tcgcacataa cggcttttta tagttttttg tcaaaaaaag gcagaaggtg
    23701 ccgactcaaa aaaataagtc gaggttggga cagaagtgtt taactccgag aaataagaag
    23761 aaattcacga aaattgcttt tcaaattttt gtgaatttcg gcttatttaa gaagaagttg
    23821 cttctgctcc caccgtttat tcgtttttaa agaatgagac aaaagtgttt cttacttttg
    23881 tcccacgctc ttattcatgc tcagagcgcc aatagataca aaatcatagc caaagtttca
    23941 cgaaccctag ctgtcaaaga atcgatattt tattttaata taggctattt taaaataaat
    24001 ggtttaaggt attcctctac aaaagccact ctatcaatta tcatgggttt aaaataagaa
    24061 gtgatggaga taaccagatt gttcttagcg tcaatataaa gaacatttcc actattacca
    24121 attgctgaat atatttcgtt ctcagaatct aatacccacc ataaatagcc atatctcata
    24181 ttttgtaagt cttctaaagc actggttcta ggtttgaaca tctcgtttat ccacatgggt
    24241 ggtacgattt gttttccttt atacatccct ttatttaggc ataatacacc aatttttgcc
    24301 agatcacttg ccgataaaca caaaccatat cctgccgtcc cgatatttag tggatcacaa
    24361 aaccagacat tggttttagg agacttatcc attgtaaact gcttatgttc ttcagctgtt
    24421 tcagcataat aattgacatg gtttggtatc cctataggat caaatagata cttattggca
    24481 aaatcaacag ttgttagctt acttgtctga tacaacaagc cagttaatat gtgtaaacag
    24541 acagtttgat agttgaattc gccggttata ccttttcttc ctcccaataa atctagtgat
    24601 gtttttgtcc aatcttcact tgaacagact tttgtccatg gatcgccttt acatttgtag
    24661 ggtgctttca ttgttagcaa gtgcttgatt gtaatagcgt aaatagtttt ctccccgcgt
    24721 ttcactttat actctggaaa ataatctaat actttatcat cgatactttt gattaatcct
    24781 tgctctaaag caatgccaat cagcaaagat acaatacttt tcgttgctga catgatatga
    24841 caagtatcgt cttttttata accattccat tcatctgtat aaacttctac cccatcctta
    24901 tatgcgacaa tttgacatat atttggttgt cgaaaaccaa taaaatcatg caactcttgt
    24961 ttattcataa gatattacct cactttcttc tataagcata atgtcattaa aaatcgattt
    25021 caattttttc gattcttcac gtaatacagg tattcttatg gactagacag cgctttggga
    25081 agatggattc tctgaaaaat ataaaggttc actttatgag tggataaaaa aagcagcaaa
    25141 tacttcgact tctttaattt accaacatta gtgaaaatcg attcatctgg agatttgtaa
    25201 tttagagtcc ttgtcttgat tagaggtgta taaagctttt tattagacat ttttaaattc
    25261 cattacaatt tgcttataaa ctaggttttt aaaggtgact atggtaagat aatagagata
    25321 ttattttgtg attaggagtg tatatatgaa taaaaatgga gaatggagtg ctgcacgacg
    25381 gatattcatt atattaatgg atgttattgt atacaatgca gcaatatata gtagtttctt
    25441 actgaagttt cgtggagaga tcccattacg aaatttagaa actttccaac actcagcaat
    25501 tattatttca gtgattttta ttgcgttgaa cattttgttg ggtgcatatg ttttttataa
    25561 tcgaatgatc agtgacatcg tgtttgtcac agtcatcggt caggtattga tgacactagg
    25621 gatcatggta gtgacttttg cgggccgctg gtttgctttt ccacgtcttg ttatcttact
    25681 ttcatttgtt ctcggtacgc ttttcttaag tatctatcgt attctcgtat ataaacttta
    25741 tttacgcgta agtcgagata aaaaggtcgt cattattggg ttagaaaagg atgtcgcacc
    25801 agcaatccaa aattttggtt caaagaaaaa caacaaacat aaagtcgaag cagtcattct
    25861 tgataactat tatgcaaaca tcaaaaatat gattaaagac atcgatattg tctatcttgc
    25921 ttctcatatc gaagaaactg aaaaaatcaa aatctatcaa ttagttacta aaaatgaaaa
    25981 aaaattgttt ttgaatacaa catttgaaaa cttgacattg atcaatccaa atattatgaa
    26041 tttcgaagat gaaagtatta ttgaagcatc tggatttaga atccaacctg aatatgaact
    26101 attcaaacgt ttgtttgata tcatcgtttc acttgtttta ttagttattg cttcaccatt
    26161 tatgttattc acagcaattt tagtcaaagt gacatcaccg ggaccaatca tctataaaca
    26221 agtgcggatc acaaaaaatc aaaaggaatt ttcaatctat aaattccgct cgatgaccgc
    26281 cacagccgaa gcaaaatctg gaccagtttt agctaagagc aatgatgcac gagtaacgcc
    26341 tgtaggtaaa tttattcgag ctgtccgctt tgatgaatta ccacaaatct tcaatgtgct
    26401 gaaaggcgat atgtcgatcg taggaccacg tcctgaacgt cctttcttcg tcgatcagtt
    26461 caatgcagaa aacccttatt actacttacg gcataatgtt cgtgctggta tcactgggta
    26521 tgcacaagtc tatggaaaat atgtctctga ttacaacagt aaattacgtt ttgatttact
    26581 ttatatcaaa aaatattcat tgatgcttga tttaaagatc ttgctgcaaa ccatcaagat
    26641 cctatttgat aaaatgtcct ctcaaggact tgaagaagac gaaaatgtga tctcattggc
    26701 agaaattgct tttccgaatg aaaaaatctt tcgctagttg ggaggcgtaa aaatgaacaa
    26761 tccgaaattc agcataatcg taccagtata caatgcagaa gcaactattg gtcgaacgat
    26821 tgatattcta aaagaattgg attatgaaga gtacgaagtc gttttagtaa atgatggatc
    26881 aacagatcaa acacaggaaa tcattcaaga actgattgaa aatgatgaac gttttgaatt
    26941 gattactacg accaatcaag ggcccggatt agcaagaaac gaaggaataa gacagtcaaa
    27001 aggagaatat ctcttattct tcgatgcaga tgacacgcca gtaaaaacga tcttgtcaga
    27061 ttatgatcat ttgctagaaa agagtccaaa agcggatctt gttgttagtt cgtttgtttt
    27121 tcgtacaatg gacgagggaa gaatcgtttc cgaaaaagaa aacttagtta cagaatgtca
    27181 ctatctaact cacgaagagt ttataaaaga catgtatcag ttaatgaatg aacagttgat
    27241 gtatgttgta tggaacaaat gctatcgtag cgatattctg aaaaataacc aaattctatt
    27301 taaaacttat aatagttgtg aggatcgaat atttaactta cattattatg aatactgtca
    27361 tcaagtgatc atgaacccaa aagtagaata tatttatgaa tttgaaggtg ggaaggggat
    27421 cactaatcaa taccgaccta acaagttcga aacatttaaa gaattctatt cattagcaaa
    27481 tactgtaaca gacgatatta ataaagacgg tatggcggcg cttcttttaa aagggacaac
    27541 ttcagttatt ttttctattt atagtacctc tgcaagaact tcaaaagaga agaaagacga
    27601 agcaaagcag atattgagtg atccagtgat ccgagaagcc aagcaaattg ctaagacgga
    27661 ttcttcagca aaaaaaataa caaagttgct ttacaatatg ccagctccac tatttttatc
    27721 tgctgtaaaa gttggaagct tcgttgaagt gaaaatgcca gggttaatgg ctgctttgaa
    27781 gcgagtatat tagttaatgg aggaaaatat aatgaaaaga attttgcatt ttcagggacg
    27841 aatgggttta ggtggagcag agtctttcat gatgaattta tatcgtaaaa ttgatcgtac
    27901 aaactatcaa tttgattttt tgatttatga agattatgca gatgttcaag actatcattc
    27961 agagattgaa agattaggag gccgtatttt tgttgtacct aatcctaaga aaaatatttt
    28021 taaatattta atagaagtga ataagttact aaaaaaagaa tctttttcta tagttcacaa
    28081 tcaggtttat tttggcgggg gaattaattt atggttagcc aaaaagaatg gaatcagaca
    28141 gagaattgct catagccatg caactgaaga tggaaaatct caaaatattg taatgaatgt
    28201 attgcgtaaa tttttaacca aattgttgtt acaaaatgca acagactacc ttgccgttag
    28261 tcaagaagct ggtgaaagtc tgtttcaaaa ccatcccttt gagatagttc ataatggtat
    28321 tgatttagag ctttatagta aaaattcaga agctaaggtt aataagagaa aagaattgga
    28381 tatctcaatg agtacttttg ttgtgggaaa cattggacga ttagagaaac aaaaaaatca
    28441 atcttatttg ttagagattt ttttagaaat actaaaaatg aattctgatt cttacttgat
    28501 tttagtaggt gaagggtcac ttagaaataa actgaaagcc aaagcagtag aattggatat
    28561 agagaaaaga gtaaaatttt taggagaaag aaacgatatt cccgagttat tatctgtgat
    28621 ggatgttttt gtaatgcctt ctctttatga aggtctgcca atggttggat tagaagcaca
    28681 agcagcaaaa ttaaaattag tcttagcaga tactatttcc aaagatacaa agttaacaaa
    28741 taatgtggat tttgtatcat tagatgaaac accacaagaa tgggcaaaaa aagtaataaa
    28801 agagccgtat gctaataaaa taacacaaga actaaagagt tatgatgttt cgtacactac
    28861 tgaacaaatg gagagtatat atagtagaga tcattaaaaa tgttcaagat attttaggag
    28921 tgattatttt tgaaaatatg ttatttgata ttggcataca atgatattgg caacttaaag
    28981 agattggtca atcgattgaa cgaaaatgca gtttttatta ttcatataga taaaaaaact
    29041 gatatagaac catttattga agcatttaaa tcatatgata atgttgaatt tttatgtaat
    29101 aaaaaccgtg ttaaaattta ttggggtggt ttttccattg tacaagcaga aatcaattta
    29161 gtaaagagag cattacaaaa tgagaaatat ttaaaatatg tattattatc tggtgctgat
    29221 tatccaatca aagataatga gtatatttat aattatttta aaaagaatag ttcagtggag
    29281 tttattcgtg gaatagatct agatcaaatt aaacataaag aattttatta taaacatata
    29341 gatgtatatc aaaaacatga ttatcctaga attaatagga ataacacaac tgcatttaaa
    29401 atttttagag ctataattaa tcgttgtctg aggatgatta aacttccccc aaaaattaga
    29461 caccataaat ttgatttgta tcatggttct caatggtggg ctttgtcgaa agaatgccta
    29521 acagaattaa tacaaatgta tgaacaaaat caacaagact acctgaattt taaaataggt
    29581 atgtttgccc cagatgaaaa attttttcat acactttttt ttaattcctc gttcaaaaat
    29641 aaaaatgtta ttggaggacc agatttacca cttgaattga aaaatattga agaaactact
    29701 ttacaaactt cgaaattagc aaatattcat attattgatc cttccatgaa caaatggttt
    29761 gatgaaaggg atatagatca aataaaagaa agtgaaaaat tatttgtaag aaaagttaaa
    29821 tcggattact ctgataaact attaaataga attgataaag aaattttgaa agttggaaat
    29881 atagatgaat agaatagtac ctgttttaga gaagagttta ttaagtcgag atgcgtttcg
    29941 agtaataaat ttaatcattt gtctgcttta ttttatgcct agtactttta atgtaactaa
    30001 tatcccgatg aaaatagctt ttgcttgggg tggaattata gttttgtatg aggtttttat
    30061 aaagaaaaat ttttttaaaa tgcgtttttc ttggctactg attggagcaa tgattagttt
    30121 tatcttaact ataatcatta acagagaaaa ttttttaatt ccatctattt ataattttgg
    30181 atatttgctg attactttga tagtaatttt tcctgcagac ttttcacaaa gtgaagagga
    30241 aaaaaagaaa aaaatggtgt attttaataa tgttttcatt atcataattt ttttagctgc
    30301 gctaatatct atctatcaat ttattttttt gatttcttat catgtcccaa caggaacgcc
    30361 aggattactt gcaagacaag gttttattga acatcggtta tttggtgtct atacaagtcc
    30421 aaatgttgga gctatgtttg gatatacttc tattatttta agtttaatta gtgtattaat
    30481 ttcaaaaata acaaaaacga ttcgtgtttt ttatatgatg aattttat
//
LOCUS       DACDLI010000029        29110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDLI010000029 DACDLI010000000
VERSION     DACDLI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.029"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IV353_001338"
     CDS             complement(<1..1030)
                     /locus_tag="IV353_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9009598.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IV353_001339"
     CDS             complement(1112..1801)
                     /locus_tag="IV353_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9009599.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IV353_001340"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IV353_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9009600.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IV353_001341"
     CDS             complement(2328..2831)
                     /locus_tag="IV353_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9009601.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IV353_001342"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IV353_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9009602.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IV353_001343"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IV353_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9009603.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IV353_001344"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IV353_001344"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9009604.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IV353_001345"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IV353_001345"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9009605.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IV353_001346"
     CDS             complement(6443..7462)
                     /locus_tag="IV353_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9009606.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IV353_001347"
     CDS             complement(7635..8522)
                     /locus_tag="IV353_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9009607.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IV353_001348"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IV353_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9009608.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IV353_001349"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IV353_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9009609.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IV353_001350"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IV353_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9009610.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IV353_001351"
     CDS             9753..10397
                     /locus_tag="IV353_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9009611.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IV353_001352"
     CDS             complement(10453..10956)
                     /locus_tag="IV353_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9009612.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IV353_001353"
     CDS             11553..12518
                     /locus_tag="IV353_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9009613.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IV353_001354"
     CDS             complement(12617..13789)
                     /locus_tag="IV353_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009614.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IV353_001355"
     CDS             complement(13776..14921)
                     /locus_tag="IV353_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009615.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IV353_001356"
     CDS             complement(14935..15867)
                     /locus_tag="IV353_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009616.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IV353_001357"
     CDS             15984..17063
                     /locus_tag="IV353_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9009617.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IV353_001358"
     CDS             17060..17689
                     /locus_tag="IV353_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9009618.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IV353_001359"
     CDS             complement(17799..18026)
                     /locus_tag="IV353_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9009619.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IV353_001360"
     CDS             complement(18129..20717)
                     /locus_tag="IV353_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9009620.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IV353_001361"
     CDS             complement(20737..21396)
                     /locus_tag="IV353_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9009621.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IV353_001362"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IV353_001362"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9009622.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IV353_001363"
     CDS             complement(22169..23035)
                     /locus_tag="IV353_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9009623.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IV353_001364"
     CDS             23144..23785
                     /locus_tag="IV353_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9009624.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IV353_001365"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IV353_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9009625.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IV353_001366"
     CDS             complement(25226..26125)
                     /locus_tag="IV353_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9009626.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IV353_001367"
     CDS             complement(26127..26924)
                     /locus_tag="IV353_001367"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9009627.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IV353_001368"
     CDS             complement(26902..27606)
                     /locus_tag="IV353_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9009628.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IV353_001369"
     CDS             27737..28312
                     /locus_tag="IV353_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9009629.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="IV353_001370"
     CDS             28430..29107
                     /locus_tag="IV353_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9009630.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa
//
LOCUS       DACDLI010000030        27913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDLI010000030 DACDLI010000000
VERSION     DACDLI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.030"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..710)
                     /locus_tag="IV353_001371"
     CDS             complement(18..710)
                     /locus_tag="IV353_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9009631.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="IV353_001372"
     CDS             705..971
                     /locus_tag="IV353_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9009632.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="IV353_001373"
     CDS             complement(1147..1716)
                     /locus_tag="IV353_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9009633.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="IV353_001374"
     CDS             complement(1790..3079)
                     /locus_tag="IV353_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009634.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3433..4281
                     /locus_tag="IV353_001375"
     CDS             3433..4281
                     /locus_tag="IV353_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9009635.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="IV353_001376"
     CDS             4426..5367
                     /locus_tag="IV353_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9009636.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="IV353_001377"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="IV353_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="IV353_001378"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="IV353_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9009637.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="IV353_001379"
     CDS             complement(9831..12017)
                     /locus_tag="IV353_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9009638.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="IV353_001380"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="IV353_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9009639.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="IV353_001381"
     CDS             complement(12239..12679)
                     /locus_tag="IV353_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9009640.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13292)
                     /locus_tag="IV353_001382"
     CDS             complement(12753..13292)
                     /locus_tag="IV353_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP9009641.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13907..15373)
                     /locus_tag="IV353_001383"
     CDS             complement(13907..15373)
                     /locus_tag="IV353_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9009642.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="IV353_001384"
     CDS             complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="IV353_001384"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9009643.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17907..18086)
                     /locus_tag="IV353_001385"
                     /pseudo
     CDS             complement(17907..18086)
                     /locus_tag="IV353_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(18289..18648)
                     /locus_tag="IV353_001386"
     CDS             complement(18289..18648)
                     /locus_tag="IV353_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9009644.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18678..19019)
                     /locus_tag="IV353_001387"
     CDS             complement(18678..19019)
                     /locus_tag="IV353_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009645.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(19016..19690)
                     /locus_tag="IV353_001388"
     CDS             complement(19016..19690)
                     /locus_tag="IV353_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9009646.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="IV353_001389"
     CDS             complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="IV353_001389"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9009647.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22081..23271)
                     /locus_tag="IV353_001390"
     CDS             complement(22081..23271)
                     /locus_tag="IV353_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9009648.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23292..23819)
                     /locus_tag="IV353_001391"
     CDS             complement(23292..23819)
                     /locus_tag="IV353_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9009649.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23866..26655)
                     /locus_tag="IV353_001392"
     CDS             complement(23866..26655)
                     /locus_tag="IV353_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9009650.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26802..27002)
                     /locus_tag="IV353_001393"
     CDS             complement(26802..27002)
                     /locus_tag="IV353_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9009651.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27454..27774)
                     /locus_tag="IV353_001394"
     CDS             complement(27454..27774)
                     /locus_tag="IV353_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009652.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7845 a         5919 c         4904 g         9245 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttc
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
    12901 aaagataaaa attctaagta ttccctgttt agtttctctg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
    13081 gtagaagtat aatttccttc aacaatccat gacggattag ataccataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
    13201 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
    13261 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
    13321 ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt
    13381 caaagtccct tgtttcgaat aaatggtgtt caatcagctg accttcagca ttaactcaaa
    13441 aaacggcaaa atatgaggag ctattttttc gtttgttcct ctaatactca ggtcaaatcg
    13501 ctgattttac aacctcttcc ttaaacggtg ttctcctgcc aacacctcgg tcacaaggca
    13561 caatagtcaa caaacatgag aagttgtttt ttgaaattgc tccttgtgac tcggagttaa
    13621 acggcaggct cactacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    13681 gcccagcaaa acgaaaaata tgaggggcta tttttgtttg gctgttctta ttacttgatg
    13741 caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt ctgcgaaaaa
    13801 caagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    13861 aagctgacca cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
    13921 ctgtttctgg ttcagtgatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
    13981 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
    14041 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
    14101 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
    14161 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
    14221 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
    14281 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
    14341 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
    14401 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
    14461 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
    14521 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggaa
    14581 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
    14641 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
    14701 tgacataagg ggatattttt tccgctcctc caactgcaaa tactagcatc gaaacggttg
    14761 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
    14821 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
    14881 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
    14941 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
    15001 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccgcca gcaatcagga
    15061 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
    15121 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
    15181 tatccttgaa ggctgaccct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
    15241 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
    15301 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
    15361 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
    15421 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
    15481 acacatcgtt ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt
    15541 taccttattg aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt
    15601 tataatccta tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga
    15661 cgttcattcg tcctcttcat tgatcaacgt ttatttttta catgcaagct tcgcatggca
    15721 ttcaaaacag ctaatactgt caccccaaca tctgcaaata ctgcatctcc cattgatgca
    15781 aaaccaaaag cacctaaggc aagtaccaaa atttttatcc caatagcaaa gacaatgttc
    15841 tgtttgacga ttttcaatgt tttctttgct aaatggattg cttctgcaat cttcgatggt
    15901 tgatcattca tgatgactac atctgctgct tcaattgcag catcgcttcc caatccaccc
    15961 attgcgattc ctacatctgc tcttgctagc accggtgcat cgttgattcc atctccgaca
    16021 aatgcggtct ttttattgtt tctttgtaag atttcctcta accgctgcac tttatcttct
    16081 ggaagcaatt cgctatatat cttgtccacg cctacttgct tcccgatatg atcagcaatt
    16141 tttttcgaat cgcccgtcaa catgactgtg ttcttcacac cagccttttt taattgaacc
    16201 aatgcgtcta cggcatcttc tttcaaggta tctgcaataa caagataacc actgtaactt
    16261 tgatcgatcg ctagataaag caatgtaccg atttcttgac ttgaaggaaa agagatttca
    16321 aatctttcca tcaatttgtg attacctgct aatacttgtt tgccatcaac ttcagctaag
    16381 atcccttcac ctgcaaattc ttggatatta gctgttggta aaagcggacc tgcgtagcct
    16441 tctaaaacag attgagcaat cggatgtgta gaaaactgtt ctgcactcgc aacatattgc
    16501 atgaatatct ttggatcgat ttttgtatcg atcgtctgga tcgaaaagtc acctttcgtt
    16561 aatgtgccgg ttttatcgaa taccactgtc tcagtttgtg caagcaactc caagtaattt
    16621 gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa gaaactaagg
    16681 ggtacggaga tcactaatgc acatggacat gagatcacta aaaatgtcaa tgctcgatag
    16741 atccattcag aaaacgccgc tccagaaaca acaagtggag gtaaaacagc taataaaacg
    16801 gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa attttcagct
    16861 ggtgcttttt tactgctggc attttctact aattctaata atttgctgat agtagaattt
    16921 tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa tccacttaat
    16981 actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc tgacgtatcg
    17041 actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc aggcttcaca
    17101 agtacaaact gaccgatctg cacgtcttca gggttcacgg tcatcaatcc ttccgtcgtc
    17161 tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt tcttgaatga
    17221 ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac tgctacggct
    17281 tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag aaaattctca
    17341 tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc atatcctatc
    17401 agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc aaataatagc
    17461 aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct ctctttttca
    17521 tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc tggttctaat
    17581 tccttcacta ctttttttat ttcttgttca atcgtagcca aatgatcttc ctctgcgtca
    17641 atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt gatcttctga
    17701 actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa tctgtaactt
    17761 ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact aattataaat
    17821 gaacggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc tgcatcggaa
    17881 aatttcttcc atacagatgt tttggcttat tctttctttt tcttttcgat cattgccact
    17941 gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact aagtttgaag
    18001 cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata tcttttttca
    18061 atccctgcaa aatgtccttc ccagatattc gaagctccac tacgctttca aaaatataga
    18121 aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca tataaataaa
    18181 agacgtaaaa gtcacatgga agaacttata caggagaacg catagagtca ggtgaaatac
    18241 atgtatccca cctgactcta tgctttatct aacctttatt attctctttt atacaatttc
    18301 tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg caaaaggtat
    18361 ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag caattggtag
    18421 gcctgaaacg atcagccaga tgatattaac aatcaatgac atgccagact ggtgataaat
    18481 tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt gcataccgat
    18541 tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc taagaccacc
    18601 gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg tatttctcct
    18661 tctattactg tttttgatta aaactgggca aaaaatgatt ttattcttct ttttttagct
    18721 tccagtatca aatattctgc aaattttttt tggatttctt ccgtattttc ttttgttact
    18781 aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc tgactgttgc
    18841 acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac gagcaatagt
    18901 ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc tggatgatca
    18961 gtaatcagct gattgatttg aggtagatct tcttttactg cccaccgtat ttccatcatt
    19021 tctcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg gcaattgcat
    19081 ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc ccatcttctc
    19141 ttgtatattt cgaaaaacca taaacaatcc ctaaagcatt gaatatacca tctaaaggaa
    19201 ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta gggtctaagt
    19261 ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc atcaagatga
    19321 tgttgcagat tcccactgtt tgaagttcat ttagtaattt tcgataaacg gattcaaatt
    19381 gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc caggtatcat
    19441 tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct tcttgccagc
    19501 gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat tcaaattcca
    19561 tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct ttacctagat
    19621 cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct atcaccgata aataatatac
    19681 gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt ccttatatta
    19741 tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga caccagccta
    19801 gtcccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg ctattttttt
    19861 cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga tcgatcacac
    19921 ctaagcttcc gtcaatcaga ccactgatga cgccatcaga acatatattt ataggctcat
    19981 ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt gggatagatt
    20041 ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggattat
    20101 tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg
    20161 tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat
    20221 taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat
    20281 agcaatttct tttgctttct tctcttgtct gctggaaact tcctttatcc cgtattttaa
    20341 aaaaagaact gcgagagttc gcagttcttt tttagaataa acggtgctct ccggccaact
    20401 cctcggtcac aagtcacaat agtcatcaaa catgaaaaac tgttttttga aattactcct
    20461 tgtgactcgg agctgaacgg caggcgcaca acctcttcct taaacggtgt tcaaaagcca
    20521 gtttctcggt attaagccaa atttatgcaa aaatgtggaa agccatttcc acaaaatttt
    20581 tcttaatact cgaaactaaa cagctttttc acaacctctc cactaaacgg tattcaaaag
    20641 cttacatctg cggtaataag tcccgccaat tcaaaaacat gaacagctgt ttttgcttgg
    20701 ctgttctaat tacttgatgc agaacagctt tttcatcacc tcttatggat aaattcggtt
    20761 caacaaacgt gggaatggaa tagcttcacg tacatggtcg ataccagcaa tccaagtgac
    20821 tgttcgttca agacctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc
    20881 aagataccat gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa
    20941 atcatagtct gttgcccgtt cgcttccgcc aataatttca ccgtaacctt ctggtgcgat
    21001 catatctgca cagatcacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt
    21061 gatggctttt ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt
    21121 ttcatgaggt gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag
    21181 taattcgatc gcatcatcat aagtaatacg agggaatggc aattttgtat aagtttttaa
    21241 gacttctttg tctcgtccta gtacatctaa tgcatattcg caatgatcta aaacattttg
    21301 tactaagaat gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc
    21361 catttctggt tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc
    21421 acggaaagtc ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta
    21481 taattggcca ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc
    21541 cgtagtgcct tctggtgcag aaccagtcaa gatcggcgga tcaattttga caaaatgatg
    21601 ttgattaaag aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg
    21661 ttgtcttgaa gaacgcagcc ataaatgacg atgatccatc aagaagtctg ttccgtgttc
    21721 ttttggtgtg atcggataat cgtggctctc gcttaccact tccacatttg taattccgat
    21781 ttcataacca aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt
    21841 ttcttggtgt agatttttag ctgtttggaa aacttcttcc gacacttcgc tttttacaac
    21901 tacaccttgg aaaaaggctg ttccgtctct taattgtaaa aatgcgattt ttccgcttga
    21961 tcgcttattt gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc
    22021 gatgatttga atttgttcca cgtatgactc tccttttata ttccttgaat ttctttagga
    22081 ttaattttga cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt
    22141 agcgcgatca gtagcataac tcatgcggac attttctggt gccccaaagc cttcgccagt
    22201 cacaagcgct acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata
    22261 tttacacatg cgtagcgttt cttttacatt aggaaaaaga taaaaagctc cttgtggctt
    22321 ttcaagagaa acacccggca gttctgccac aagcgaatat aacgtattca ggcgttcttc
    22381 aaaggcgttc cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt caacagctgc
    22441 atattgactg actgctgtcg gattactggt tgactgggaa gcaacagcga tcattccatt
    22501 gatgatcaca ggatttccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt
    22561 agacacgccg ttgatgatga tagtctgatt tttgattgct tctgaaatcg ttgcaattgg
    22621 agtaaactca ttgccattat aaactaaacg accataaata tcatcagcaa ctatcaaaat
    22681 atcatttttt actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc
    22741 agtcggattc gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgcttgttc
    22801 tagctgttca acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag
    22861 tccttctgcc agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac
    22921 ttcatcttgc gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc
    22981 tgttacgact gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac
    23041 gattgcctgt cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccgtt
    23101 gcggattgct tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc
    23161 cacagtcaaa ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc
    23221 tgccaaagtg acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat
    23281 tcctttacct tttaaattcc cttgattgtc ttgacttcat ctccagattc aaagccaatc
    23341 aggtaataat tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca
    23401 tcatacatcc ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc
    23461 tgttttgctt cgttttcagt cagtcccttt gtttgatcca atacttttac tttttcacca
    23521 gatttaggaa tgataacggc gatttctttt cctttatcat ttgtacctgt cagtgaaaag
    23581 tatgtcttct ttcgagtaaa ccaataaaaa tgatcggctt cctcaagatt tgcatatttc
    23641 tttgcgattt ctactgcttc tttcttcgcc tgcaccgccg gacgactgga acgcaagtaa
    23701 aaaatcgagc tgatcacgat gaccgctaat aaaaatacga tcaagccaag taacacgcga
    23761 ttaatcgttt tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt
    23821 ccttttcatt atagcagatt ttaattatga aaaaagacag ctcctctaac ttccttcttc
    23881 atttttcttt aagaattcat ggatgtcctg atagatttcc tccatagcta gctctttgat
    23941 cggtactttt ttcggtaatg catggatgat ccgtgcacca tattttgcag tgactagtct
    24001 tcgatccaaa ataagcaata cgccacgatc tgtctccgtc cttatcaagc gacctaatgc
    24061 ttggcgtaac ttcaaagctg ctttaggtac ggattcctgg taaaaaggat tgatcccctc
    24121 tgattctaaa taagcgttcc tagctttgac catcgggcga agcggattat cgaagggtaa
    24181 acgtgtgact acaacgatct gcaaagcatc cccaggcaaa tctacacctt cccaaaagct
    24241 gtcagcgcca aacaatacgc ttttttctga caaaatgaag cgtttcaaaa gcttctcccg
    24301 gcttccgcca aacccttgag ctagtatttc tcgtcctttt tctagcatag acagatgcat
    24361 tttctgatag accttctgca aaacatcatg agaagtgaat aacacaagta ttggccgatc
    24421 ttcttgttcg atcagacctg taagtgctgt ctctaaataa tcgatatatg cttgattgct
    24481 gacttgatgg acggcaggtg cctcttttgg cacatagatc cttgcctgtt tctcataatc
    24541 ataaggagag gcaatcactt taagaggtgc tgcttcaatg ccaagccgct tagagagata
    24601 gtgtcgatta gggccgatct tcagcgttcc tccaatataa agaatacgat catatcgagg
    24661 ataccaaaca gtcgacggca acatagccgc ttgaaaatca tggagatgaa aaatcatctg
    24721 ctgcgctgtg ttcccaggaa cgaaccaatg gatcaaatga tctcgccagt cttccagcca
    24781 ttgatccatc agcaagtact gcttgtctaa ttgatcaaat aagctatata aacttgctaa
    24841 atcgatccgc atctggtttg tccagctttc ttggataatc tcaaaagtca gacgcatttg
    24901 tgactgtagc tgctggatct cctgataaaa aagctgcagg tgatcaatgg ctgtcccaga
    24961 ttccagtggc agctgatcca acatctcttt tgtcatcatg acttcttctg gatactgtcc
    25021 gttaggttgc ttgccgctca ctaaactcat taatccttct gccatacaaa tctgtgcttc
    25081 gcatagcgca gaaagttctt ctcgataaat cgataaccag cgtaaggctt catgttcttt
    25141 cggtaaatag ctttgtattc ggtcaaacag ctgatttggt tcttctaatt gctggatttg
    25201 tttatggaaa tagttaatac ttaatcgttt gtcatttact ttttcagcga tttccggcaa
    25261 atgatgtact tcatcgatca acaaaaatgg actagctgga agcaatggtt cttttcgctg
    25321 tgtttcttgt attaaaaacg catgattgac aatcaaaaaa ttactttgcc tgattttctt
    25381 ttgcaagaaa cgaacaaagt cttgttcgta aaatgactga tttcttgcta aaaatccaat
    25441 acctctgtgc gcgatatctg caaagaatga atggttcaaa tttgttaaat gtaattcatc
    25501 caaatcaccg gtcttcgttt gcgtcagcca aactaagacc gccatttgat acaaagcata
    25561 ttgtttttgt tctggcggtt ccacaagtgt gcctttaaaa cgctgtaaat caatatagtg
    25621 gcgatagctt ttgataatta caggttgtat gggctgatct agcaattgat tcagcaatgg
    25681 aatgtctttt tctatgatct gctgctgcaa aactagcgaa acggtactga tgacagcggg
    25741 tttttcaggt gtagccaaat aactcaatgg cagcagataa ccgattgttt tacccattcc
    25801 tgtagctgct tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagaccgc
    25861 attcatcagt ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt
    25921 tgcttgtttt tttcttggat atgtccgctc gccataatgg acggatgtga atagttccac
    25981 ttcttttttc tgcaaagcta gcccatcgac gatttctaat gagggatcaa gtggctctgg
    26041 tttttctttc atttcttcaa tcacatgata aatgaaacga ctcgtatcca tacctgtgtg
    26101 tctacttaat cgcgcaatct gctctaacgt gatgattggc aattttctca tccgttcttc
    26161 gatcagcaaa agaagttgac ccgtcacttc ggcatcacta tctgcctgat gaggattctc
    26221 atgaataaat ccaaggcttt ctgacaaatc agctaagcga aagcttggtt ccgtcggcaa
    26281 gaaaatttgt gcaagttcta ctgtatcgat gcctggtatc ttcaaactag gaagtccgca
    26341 tcgcatgagt tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat
    26401 ggtgtctgcc aaaagattgt aaatcgtttg ggcgacatct tcgaaataag gcgctttttg
    26461 gacgcgttga ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg
    26521 atgaatatcg atcgaaaaac gtgaagtgat ccgacctgat tcaatcatga cgcagccaaa
    26581 ttggatgatc cgatcttctt ttggattcgt cccagtcgtt tcgatatcaa cgactgcata
    26641 agttcttggt ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata
    26701 ctacaaagtg cagaaaaata ctctcatcgc tttttaaaga aaatagacaa aaacgcccag
    26761 acacgcaaat gtctgggcgt ttatttttta gtataggtta attaagcttt agtaacgttt
    26821 gttgcttgag gtccgcgttg gccttcttca acatcgaaag ttactgcttg gccttcttct
    26881 aaagttttga atccgtcgcc ttggatagct gagaaatgaa cgaatacatc gtttccgttt
    26941 tctgcagtga tgaatccaaa tcctttttct gagttaaacc attttactgt acctgtttcc
    27001 ataataaaaa tcctccttgt gtatcaacac atattttgca attacttgaa tggtgtatat
    27061 ctggaagatg tttatttgaa acaagctacc acagattacc gaacaaaaac tactcctata
    27121 gtatatcata ccgtttacaa aaaagctaga gtttttcgac tttttttatt aaaaaaagtt
    27181 ttctcagcga ttacataaag tgaaaaaccg aacttttttt aaaataaacg acatattgtt
    27241 cagttggtgt ttttttgctc tgatgttctt ctttaaacta aaaaaattat tctttctgac
    27301 tacttatcgg ttctgtttca acggtcgagg ctggggaaga ttctttagta ggtctgatgt
    27361 tctcatggta tttctgatag cctatgacag ttaccagaag aacaaggcaa accaatacga
    27421 tcaacgagat tagtttttct tttttgctca tatctatacc tcataaataa caggatgatg
    27481 tccatcatat ttctgagtat gagtaaaagc aaaagctgca gctgtagaat gtaagaaatg
    27541 ccagcaactt tcataatcac aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa
    27601 agaccgttct tctgtttcag gaatcgcaga atcaagatct gttgtctgta cataaacatt
    27661 tttgtacaga cgtgtgcctg taataggaga ccgctgtgtt tcttcagcaa aaactggctg
    27721 acttgttaaa cttagggata gaaacagcaa acaccataac agtctttttt tcataaagac
    27781 ctccttatcg ataagttttt cccaattaaa atcatatctc aagaacgccc gttattcaca
    27841 ttttgttcat aaatgatgtt ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc
    27901 acaacctctt ttt
//
LOCUS       DACDLI010000031        27901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDLI010000031 DACDLI010000000
VERSION     DACDLI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.031"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(547..909)
                     /locus_tag="IV353_001395"
     CDS             complement(547..909)
                     /locus_tag="IV353_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009653.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(983..1363)
                     /locus_tag="IV353_001396"
     CDS             complement(983..1363)
                     /locus_tag="IV353_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9009654.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1445..2620
                     /locus_tag="IV353_001397"
     CDS             1445..2620
                     /locus_tag="IV353_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9009655.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2697..3281)
                     /locus_tag="IV353_001398"
     CDS             complement(2697..3281)
                     /locus_tag="IV353_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9009656.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3482..4480
                     /locus_tag="IV353_001399"
     CDS             3482..4480
                     /locus_tag="IV353_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9009657.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4592..5101
                     /locus_tag="IV353_001400"
     CDS             4592..5101
                     /locus_tag="IV353_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9009658.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5131..5790)
                     /locus_tag="IV353_001401"
     CDS             complement(5131..5790)
                     /locus_tag="IV353_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9009659.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5792..6556)
                     /locus_tag="IV353_001402"
     CDS             complement(5792..6556)
                     /locus_tag="IV353_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9009660.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6564..7241)
                     /locus_tag="IV353_001403"
     CDS             complement(6564..7241)
                     /locus_tag="IV353_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9009661.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7278..8663)
                     /locus_tag="IV353_001404"
     CDS             complement(7278..8663)
                     /locus_tag="IV353_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9009662.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8873..8957)
                     /gene="ffs"
                     /locus_tag="IV353_001405"
     ncRNA           complement(8873..8957)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV353_001405"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8980..9495)
                     /gene="tadA"
                     /locus_tag="IV353_001406"
     CDS             complement(8980..9495)
                     /gene="tadA"
                     /locus_tag="IV353_001406"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9009663.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9602..10459
                     /locus_tag="IV353_001407"
     CDS             9602..10459
                     /locus_tag="IV353_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9009664.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10540..10875)
                     /locus_tag="IV353_001408"
     CDS             complement(10540..10875)
                     /locus_tag="IV353_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009665.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10942..12312)
                     /locus_tag="IV353_001409"
     CDS             complement(10942..12312)
                     /locus_tag="IV353_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9009666.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12390..12926)
                     /locus_tag="IV353_001410"
     CDS             complement(12390..12926)
                     /locus_tag="IV353_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9009667.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13235..14716
                     /locus_tag="IV353_001411"
     CDS             13235..14716
                     /locus_tag="IV353_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9009668.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14819..15526)
                     /locus_tag="IV353_001412"
     CDS             complement(14819..15526)
                     /locus_tag="IV353_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9009669.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15605..15943)
                     /locus_tag="IV353_001413"
     CDS             complement(15605..15943)
                     /locus_tag="IV353_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9009670.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(16054..17016)
                     /locus_tag="IV353_001414"
     CDS             complement(16054..17016)
                     /locus_tag="IV353_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9009671.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(17031..18362)
                     /locus_tag="IV353_001415"
     CDS             complement(17031..18362)
                     /locus_tag="IV353_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9009672.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18370..19050)
                     /locus_tag="IV353_001416"
     CDS             complement(18370..19050)
                     /locus_tag="IV353_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9009673.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19269..19748)
                     /locus_tag="IV353_001417"
     CDS             complement(19269..19748)
                     /locus_tag="IV353_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9009674.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19875..21554)
                     /locus_tag="IV353_001418"
     CDS             complement(19875..21554)
                     /locus_tag="IV353_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9009675.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            22102..23604
                     /locus_tag="IV353_001419"
     CDS             22102..23604
                     /locus_tag="IV353_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009676.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23617..24279
                     /locus_tag="IV353_001420"
     CDS             23617..24279
                     /locus_tag="IV353_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009677.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24380..24583)
                     /locus_tag="IV353_001421"
     CDS             complement(24380..24583)
                     /locus_tag="IV353_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9009678.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24694..25878)
                     /gene="iadA"
                     /locus_tag="IV353_001422"
     CDS             complement(24694..25878)
                     /gene="iadA"
                     /locus_tag="IV353_001422"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9009679.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(26035..27774)
                     /gene="yfcC"
                     /locus_tag="IV353_001423"
     CDS             complement(26035..27774)
                     /gene="yfcC"
                     /locus_tag="IV353_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9009680.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8296 a         5812 c         4714 g         9079 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      301 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      361 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      421 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      481 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      541 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      601 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      661 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      721 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      781 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      841 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      901 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      961 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
     1021 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
     1081 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
     1141 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1201 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1261 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1321 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1381 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1441 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1501 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1561 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1621 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1681 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1741 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1801 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1861 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1921 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1981 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     2041 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     2101 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     2161 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2221 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2281 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2341 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2401 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2461 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2521 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2581 acttaatcga atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa
     2641 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2701 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2761 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2821 tgtcggaaat ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg
     2881 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2941 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     3001 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     3061 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     3121 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     3181 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3241 ggtccttttt cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt
     3301 tgcatacttc ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt
     3361 tttcttttat ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca
     3421 aacatttttg agaagcattc tcaattaaat tctgctatag tgtgaaaagg gaggaatttt
     3481 tatggaaatc tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt
     3541 ttacgcagga atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg
     3601 tcaattagca actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat
     3661 aaaagcaagt gaattaatcg atcaattaga aaaacaatta actactttta accatacatt
     3721 ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac
     3781 aaataaaggc atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca
     3841 gccaagaaaa ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt
     3901 cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc
     3961 tgctatcgat tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg
     4021 tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa
     4081 cttgcttact ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat
     4141 ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt
     4201 caattatgga ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag
     4261 aaatgccatt actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg
     4321 tgacatctgt acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc
     4381 tactgcagtg aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca
     4441 cagcacaagc ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat
     4501 ttcaagttaa acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata
     4561 gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga
     4621 aaaggcaaga gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt
     4681 catgttttta caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt
     4741 caacagcgtg ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga
     4801 tatattagca gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga
     4861 aggcctttac ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat
     4921 cggctttacg aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa
     4981 cagccacgat ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc
     5041 tgctgctgcc agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata
     5101 attcaacaaa aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtttgttt
     5161 caagctattt tttcctaaga aaagaaaaac agcaaaaaat aattctaata atacaaaaaa
     5221 taagataaag ctatgcatga aaaattcttc tggaagtaca ttgatgattg gatcagtaac
     5281 gggatcgaat aaccagtcat cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg
     5341 atcaaatcct atcatcatga aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa
     5401 cggacgaatc aaccgccaaa gccgatcatt cttttgcaaa tatttgataa acaaaatact
     5461 cgggatcaaa gtgatgaaaa acacagcata atccactaaa aacaaaattt tcacttcata
     5521 aaagtgatgg gctccgctag cagaaactgg gaaatctggc aagctgagga tggttttgaa
     5581 gggattattg agataattca ttaggtgatc aaaatttttc agtaacgtct cttgatctaa
     5641 agaagtataa tccaaaattt gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt
     5701 gatcgtcaca ccaactgcca gactgatcag tgtcagaaat aggctgaaca atccagcata
     5761 ttccatccac tgccatctcg aatttttcat attagaagct ccattcatcc agtgattgtt
     5821 tcacataggt agctgggata ggcaacccag gtacatcttc ttcttttgta aaacctgata
     5881 aaacgagcaa agtatctata ttattgcgta tcccagcttg gatatctgtt tcatagttat
     5941 ctccaaccat gattacttct tctttttgca gccctaatac ttcaacggct ttatccataa
     6001 tgatggcttc tggttttccg atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag
     6061 agatgactga ccctgctcca ggaagaaggc cgcgttcagt aggtatattc ttatctgggt
     6121 tcgttccaat gaaagtagca cctttttgaa tagcaagtgt tgctttaact actttttcat
     6181 aggtcagata attatctaag cctaccacta catagtcagg agcctcttct tcccatgtaa
     6241 atccggcagc taaaatcaag tcaatcaatc ctgcttctcc gataacatag acttttttcc
     6301 cttttccatc tgctttcata aaatcgattg ttgccaaagt agctgtgtag actgtctctg
     6361 gagctacatg aatgtcaaac tcatctgcta gacgattagc tactgtttct ggtgacttag
     6421 tcgtgttatt ggtaacaaac aagaaaggta attttctttt ttgcaattct tccacaaatc
     6481 gtttaccggc cggtatgggt tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat
     6541 atcctttata gtccattcat ccactactct tccctttttc tgatcgtaaa tgctgttttt
     6601 gttttttcta cttgtttagg ttgttcttgt ttttttactt tttcagaggt ttttgctgac
     6661 tctgtttttc gatttttaat aaccggtttt ttgcgattag atgcgactgg tccttttttc
     6721 tcatcgatat acgcacgatt atggtttttt ttcttttttc ggcgctgagt tgtcttttct
     6781 tttttaccgc cgattctctc gatgacaaaa tacgcacaac caaaattgca atattcgtaa
     6841 agatagtctt ccaaggaatc gattcgctga tctggatgcg cccgtcgatt atccgcttgg
     6901 aaaaatcctt ttaggcgtag ttgttcataa ccccaatctc ctacgatata atcatatcgg
     6961 gctagtacat cactgtaacg ttctccaagc ttttctgcat taaatccttc acgataatcg
     7021 gacacaagcc gataatgtct ttcacctatc aaaaaattac tgtcatctaa cagtgtcact
     7081 agctcgcctt tgactttttc tgctttaggc tcctcaacga tttcttccaa agcagctgtc
     7141 agttcttcgg tgactgttgt ttcctgttca gctgatgatt ttttcttagc aatttctgtt
     7201 ttatgctcaa cgttctgctg ttcattttgt tttgcagaca tgcgttcacc acctatcttt
     7261 tttattatac cttgttttta tttgattggg tagtgagctt tcaagtaatc tcctaccaca
     7321 cttcggataa attcagggaa caaaaattca ttttttccag caatctcaat cgttggaaaa
     7381 aatggaacaa acatcaaatg atctacggta gtaaaagtat aatttttatt cagatcgatt
     7441 ggcttgttct tccaaaagac ttgatggtta atagaatcat atgtgattcc attatagacc
     7501 atctgcccaa aaatcttacc gcgaaatccc attcccttca taggatagtt acgtaaaaag
     7561 ttacgatttt tttctatttc taaaactaag cggatcagat catttccacc caatgtgaca
     7621 tttaacaagt gcatcggatg cggcaacgca gtgtgaagct gatcctgatt gacccgccct
     7681 tttggaattt cagttaaaaa cagaccactg tttaatattg ctgcttccgt ctttcctcgt
     7741 tctttcaccg cctcaaaggc tacttggata aacgaatgct cattgaagat atctagactc
     7801 agagaaaatg gcaaatctgc tacttctttt tcttttaata attcatgccc acgttctaga
     7861 taactattta tttcttgttc atcttctgta aaagctgtca ttgtttcggt agggatggct
     7921 ttggctgaat gcttaaggat ttttttctct tcatcgatgg ttaaatggac ttcaccgaca
     7981 tactgcccgt actttccagc ggctgctatt tggacatcat tgattacgtg accttctctg
     8041 aataaatgat gggtgtgcga tcctaaaata acatcaattg ctggtatttc ttttgcaatt
     8101 tggaaatcat cctgaatccc gagatgactc atcaaaacta aaacatccac ttgtgaacgt
     8161 aaatcttcga ctaattctgg caaaatatca taaggattcc gtatgtccca gccattcgga
     8221 ttataagtaa gcggaaatgg agcagtaaat gcaattagtc cgattttcgt ctggtgtttc
     8281 gttgtaatga ttttatatgg tttcgtccat gccggtggct ggagcgtatt tttatcgaat
     8341 aagttatcca ggatgatatc aaactcagca tgatcgtata aatgattcaa ctcttctttg
     8401 gaatttccta cgccttcatt gtttcctatc gtcgctgcat cataaccaac ttcattcatc
     8461 agcaagatat tttcctgtcc attcgttgct tctgacaatg gatgccaacg atccacaaaa
     8521 tcacctagat ctactgcaat cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt
     8581 tgatccaaaa accttctgat ccttggccaa ttttctaaat gagaatgcag atcattcgta
     8641 tgtaaaatca ctattttttc catgtaccct ctcgcctctc tttatcttta tcataccata
     8701 ggcagctttt tcttccattg aaacaagctc tatttcagag tttttcctgc cttgaagcaa
     8761 attgtataag tacaatattc gataaatcag tctggctaaa aaatcaggaa cattcatttt
     8821 tacagtacta taaaaataaa aaaaacagac ccgaaagtct gttgtcgttt cattataatc
     8881 agacacatgg cggaacctgc ttagtgctgc ttccttccag acctgacacg cttcacaagc
     8941 ccaccattgt cctagccttt cggcaaagaa tagtatacct tatttttcat tcgttgacca
     9001 gtcttttttt aatttctttt ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat
     9061 cccacattcg tcttctaaaa tgccaccttc tacatatgct tggtggttaa accgctcgtc
     9121 ctccaaaaga ttcatcaagg taccggctgt tccgcctttt ggatcatacg cgccgaaata
     9181 aacttctgct acacgagaca aaatcatcgc gccgctgcac ataggacaag gctctaaggt
     9241 aacaaaaagt gcagcatctt ccaagcgcca gcttcccact ttttcacagg cttccttgat
     9301 tgcaagcatt tctgcatgtg tagtagcatc ttgcgtggtt tctctaaggt tatatcctct
     9361 tccaatcact tttccatcca atacaaccac tgctccaatc ggaacttcat gaagcatttc
     9421 tgctttcttt gcttctgcca atgcaaggcg catccatttt tctttttcta ctgcatcaag
     9481 atttgtattt accaattacc cttccctgcc tttgtttttt cttgttttac tttagcatat
     9541 gataaaaagg ctgtaactaa cttttttttc tatggtaaaa taaatacaag aggtgaacaa
     9601 gatgaaaatg gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc
     9661 atcagaagcg gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga
     9721 ttcattatct caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca
     9781 aaaaaaacac ccatggtatc actatctttt taaagatgct tcttgtgttc aagaaaaaag
     9841 ctttcgtttg atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca
     9901 agatactatt ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga
     9961 tgcgctctta gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat
    10021 ttttctatct ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca
    10081 ttcacctggc accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga
    10141 gcaaaacaat ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta
    10201 ctatacaaaa gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa
    10261 acaaaatgaa atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc
    10321 tgctgcaaaa acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca
    10381 agaacagtct ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt
    10441 gcttcatgaa cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc
    10501 ccttatttcg aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa
    10561 atgcattagc cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga
    10621 gattttcttt tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact
    10681 atctgaaaga ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac
    10741 gaattttttt actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga
    10801 tacaacgact gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt
    10861 tgttgatgtt ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa
    10921 aaaataaacg aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac
    10981 tgttcctttt acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca
    11041 tccttgattc ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg
    11101 agtgatcggc aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct
    11161 aggttcaaat cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa
    11221 accatcgaga gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag
    11281 tcttccagta gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc
    11341 ttttgtcatt tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg
    11401 tccatgaaaa gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat
    11461 acgtattgtt ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga
    11521 ttgagtcaaa ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat
    11581 ctcttcattt tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt
    11641 tagcattttt attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga
    11701 ttcttctgtc cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca
    11761 aggagcatca accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg
    11821 tgcatgattt gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat
    11881 cttcgcacgt ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc
    11941 agctaattgg gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg
    12001 ttttgctgca gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc
    12061 agcttgatgg aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg
    12121 agcgggttgt aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag
    12181 tggattccac cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga
    12241 tgcttcttcg cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc
    12301 ttcctccttc attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag
    12361 gtggctgatc tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc
    12421 aagtattcat ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct
    12481 tgagaagaat catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac
    12541 ttttcttctt tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc
    12601 gaatccatgt ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa
    12661 aaatctttca ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag
    12721 ttttcaaaat aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg
    12781 aaatgtgtat aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta
    12841 attgacttca ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta
    12901 ctataatttg ttactctccc catcattctt acctccgtct cttttcatct acccataaat
    12961 aaataacata aataattcta aaaatattat tgataattat caaaacatat ttagttatat
    13021 ccattaaaaa tcaatttttc cgccttaaaa acaagaatat cttgacaaaa taccctagga
    13081 aataaacaat agaataaaca aggtaacaaa aaaattaaat aacgttatat aaaaaagaag
    13141 gcgacactct gtgagaataa cacagactga aatgactttc cactcacagt ttttgtctaa
    13201 ctatatacaa aaacagaata aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga
    13261 gttacttgtt ctacaaatgg tcattcaaaa aaataattgt tctttacaag aattatccaa
    13321 tgacttacat attccaaaac ggacaatcaa agaactgatc cgaaaaataa atgtatcact
    13381 caagcaattt ttattgattg atgaatttat ttattctaat cataaaggag agatccaaac
    13441 caaagaaacc gatcaaacta aaaagttagc tgttttttct aagttaaaat tatgctacct
    13501 aaaagaatcc aatcgtttta actatttgat gttactaatc aattatccac aaactgctgt
    13561 accaaaaaaa tatttacttg aacaacttta tatatcgccc tcctatctcg aaaaactgac
    13621 aaaacagctc aatcattcct taaaaaaatt ccatttacgg atcctatctg taaaaaattg
    13681 ctgtactctt gaaggaaatg agttatctat ccgtctctat ctttactttt ttctttcaga
    13741 tacatttgga ggtaacgatt ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa
    13801 cagtacagat gagcaaatag aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt
    13861 aactttattc atattacgaa acaaacaaca ggcatatttt ccaaaaactt cttctgaaat
    13921 cactgaattg atggacttat tgcagcatac acatgattat tcagaatact ttacttcttt
    13981 ttcagtgagt cgcccttgtg aagagacaga aacagagaga gcctacttta attatttttt
    14041 ctattgtttt tttccaaatc aaatctcttt tcgacaaaaa caggagattg gttttttatt
    14101 ttccacaagc aaaaacctta tttgtcgaca agtcggtcaa ttgatgactt acttaactca
    14161 acagtttcct ctcaaccatt tagaaaataa aataccaatt tatttttatt ttcttgtgtt
    14221 ggcagttgtt tttttcgaac tgttgggtga atccagcagt agtttttcta cactttgctt
    14281 tccagaagcc acgtccaatc attctagttc ttttatacag gaaaagaaat taagagaaaa
    14341 aatcgataca attttttcaa aaaatgggga aagaaaccaa atcagtcaat accttagtca
    14401 attgatccaa cacttactac aaacagaaac gaaagagcag ttactcatcc atttgcagat
    14461 aaccaaacag tttacaggaa attatcatct tcaaagccat ttgcagcagt tatttcgcaa
    14521 agatgctgtt aaaattaccg catattcaga acatgctgat ttgatcatta cagatcagca
    14581 tgagaaaatt tcagatgaaa cggagacttt ctatctggat acaacgcaca atacagtgtt
    14641 atttggcaag ttaataaaag cggttcatga actgtatatg aaaaaaggtt atgagaacaa
    14701 aacaaagagg atgtgacaaa attcttgtca catcctctta ttccaaataa atggtattca
    14761 atcagctaac tctcggtatt agctcagatc aaagcgctga tttcacaact tctttatttt
    14821 aatctcttcg gttgccgccg aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa
    14881 gcgctgctgc aacataagtc agtgctgctg caaataatac gtgtcttgcc atcggtactt
    14941 cttcttccgt cagtagtccg ccttcactca aaatttggat tgctcgccga gacgcgttaa
    15001 attcgactgg taatgtcacc aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga
    15061 tattgattaa cgtagagttc cagctaagca atacacctac cataatcaag gggaacgaaa
    15121 tcatcgaacc aaagttagct gctggaacaa gtgctgcacg aatcttcaat gggacataac
    15181 ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc tacacctatc gcagcaactg
    15241 aagtagactg tgcagttgct tgagacaaac tcaacatctt attacctgaa ttgtagttat
    15301 ctgtgagatc tccggcaatt tgttgaacac cgacattatt tatctgttga ctttgtaata
    15361 tatattgtgc tgcttgtgta ccagtcacat tgtttttgct tctcacttga tcgtattttc
    15421 ggaaagtact attgacgtac gctgacgcaa ttccggaaat aactaatcca agaatgacca
    15481 aaagaaatgt cggatctaaa aaaccataca ttccataacc atacattttc gtttcactcc
    15541 ttttcgttta ctttatttta tcattagtag taggaaaaga atgtgaaaag accttgaata
    15601 ttctttaatt ctttttcaca aattccgatt tcagtttcat cggtccaaat ccttctacct
    15661 tgcaatcgat attatgatcg ccttctacta aacggatgtt cttgactttt gttccttgtt
    15721 taatggcacc tgacgcacct tttactttca agtctttaat cactgtcacg ctatcgccct
    15781 ctaacagacg gtttccattt gcatctaata ccactaatcc gtcttctatg acagtttctt
    15841 gttcttcttt gttccattcg ttcccgcatt ctgggcaaat caacaagcct cgatcttcat
    15901 atgtgtactc tgagccacat tctgggcaat ttggtaatgt catcttcttt ctcctttaaa
    15961 atctttagtg agcactattg taacggaaaa agaagatctt gaaaagatac atttgtgatt
    16021 tttactacaa aaggcacgtt ttcgaacgaa gtatcattct gtttttctgc tcttatacga
    16081 ttggcatata tcttggatct ctttatagat ttcctctact tctgctaaat cttttgcatt
    16141 cgcaccactt gctaatggat ggcctcctcc atgatggcgt ttagccaatt cattgatgat
    16201 cggcccctta gaacgtaaac gtacgcggta ataccctgaa ggctgctgaa caaaaatccc
    16261 ccaagccagc acttcgtcaa tcactccagg aagtgaaacg atcgaagctg tctctgaatc
    16321 atcgattcca tatttatcaa gaatttcttg cggaaggatt attcttcctg caccattttc
    16381 atccacctgg atattttgat aaagataacc ggaaagttta gcgactttca tcggcatttg
    16441 ttcaatctct ctgttcaaag cacttgcatc aaatggataa tttctcaata tcgaagcaat
    16501 ctctaatgta cgggacgtcg tggagggata caaaaagcgc cctgtatctc cgacgattcc
    16561 tgcataaagt aaccgtgcag ccttaacgtt catctttaaa gattcaggaa acatcaaagc
    16621 aaattctgca atcatctcac tacagctact tgcttttgta tttacccaca caagatctcc
    16681 ataagggtca tcattgggat gatggtcgat tttgatcagt ttttctgcca actgatatct
    16741 ttgatcactg attctgggtg aattagctgt atcagtcacg atgactagcg ctccttggta
    16801 gaagacatct tcaacttctt gcatttctgc taaaaagcga agcccttcaa tcgtttcacc
    16861 tacttgacgt atttcttttt ctgggaaact ttcccgtaat agttcggcta atcctacttg
    16921 tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg ataatgattt tatcgtattt
    16981 ttttattgta tctactattt cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt
    17041 tccattactt gacaaacaac gattgctttg gaaacgatgg tgttttcaat aaacacttcg
    17101 atatccagtt tagcggaacg tctgccgatt tccaatacac gtggacgaat gtctaattcg
    17161 ctttccaatt ggatcagccg caaataatgt aggttcactt gttcaatcaa gatatttcgt
    17221 ttttggttca tcaccattgt tttttgtgcg acatcagaaa tgatttcgct aagtacgcca
    17281 aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta ctacgaattt gaacttaggg
    17341 ttttccaaac gatttccttc acggtcagta tcaatcgtat ggatatttcc agatatctga
    17401 tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa gctgcatcgc tttcattaca
    17461 tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg aaacgaccgg catgacttct
    17521 agcccatccc agatcatttg atggctaaca gaggcaatac tcattgtttt ctttacactg
    17581 attggttctt tcgtcatcac ccgatcgatg atctggtttg gcgatttccc taaaacatct
    17641 tttgccgtaa caatccccat caaacgatga tggcggttga cgactggaaa acgcgaatgc
    17701 tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat ctgtctgctg caaataatga
    17761 gtcttttcag ctggcatata gatatcactg acaagcatga tatctttttt gatcaattgg
    17821 tcgcttaacg ctcggttgat catggttgcg actgtaaatg tatcataaga ggtgcgtaag
    17881 atcggcattt caagatcatc agctagcttt gcaatctcag cagtcgtatt gaatcctcct
    17941 gtgatcaata ctgcggcgcc atcttccaat gctaattttt ggacttccgc ccggttgccg
    18001 acgatcatta gtgatcctgg cgtaatatag cgttccatgg catgtttagt catcgcgccg
    18061 atgacgaatt tattcaagac cttgtctaat cctgccgccc cgcctaaaac gtccccttca
    18121 atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg
    18181 atacggatgg tccctactcg ttgaattgtt gaaactagtc cgacattttc tgcatctttg
    18241 attgcacggt acgctgttcc ttcactgaca ttcaattctt ttgcgatgct tcttacagaa
    18301 attcgattgc caacaggtaa actttcaata tattctaaaa tcaaatcatg ttttgtcgcc
    18361 attttctgcc tacacttcca gcgtttccga gacgctcatt atttttcctt cactatcttc
    18421 cagtaactct acaaaagatt cgggatcttg ttcaatgaca gggaaagtat tgtaatggat
    18481 tggaactact ttcttggcac gaattgtttg cgcagccttt gctgcatctt ctggccccat
    18541 cgtatagtta tcgccaatcg gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa
    18601 tcctagatca ctgaaataag cagtatcccc tgcatgataa atcgtttttc cttctgcttg
    18661 aataataatt ccgctaggct cgcccatata ctgaacgata ccgtctactt catagcttga
    18721 actatgctgt gcatgtacga attttaccac gccgaatgga aacgcatgtt ttccaccgat
    18781 attcataccg aatgaatcta ctcctctgct gttcgcataa tcagcaatct caacaatact
    18841 gataattgtg gcttgatttt cttttgcaat aggaatcata tctcctatgt ggtcactatg
    18901 accatgagta atcagcaacc aatctgtctt tacttcttct gcagttaagt ctgtcaatgg
    18961 atttcctgta ataaacgggt caatcatcac gcgttggcca ttttccattt caattgttaa
    19021 acaggaatgt ccatgatacg taattttcat attttacacc tcgcgttttc tctgtatctt
    19081 ttttattaaa aacgatttca tcacttgcac atcaatgaaa aaatatagct gctaaatata
    19141 ttttcattgg attgatagta cagtatgtac aaagcaattg taacagataa tacaggatgc
    19201 accaagtacc aagcagatat aatctcaagt ttgaggtctc tgctctgctt agcttctctt
    19261 ctcgtccctt aactaattaa atcagcgtaa ataggatgga cgatcttttc taaatcggct
    19321 acttttatcc gaaggcttct cccgatttca ccgcttgaca caattacttc ttctttttct
    19381 tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg cgagttttat tccaataggg
    19441 gtgtttgctc cgtggggata tcctgtatgc attaatacaa aatccattcc aggaaaacca
    19501 actttacgat ctttcgtcac ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga
    19561 atcaatgcga tgatcacccc agttttgttt cctttcacta cgagagtttt acatatcaaa
    19621 gctggatcaa tccctctttt ttgtagttct tctgtgaaag attcttctac cacatgacta
    19681 aaatcaagtg gttgatagct gatcttttgt tcttctaaat aacgttcgac ttttgtttga
    19741 ctcatcattg attcactccc tttattctat cgtttattat aaagcgaggg atgctgtatt
    19801 caaaaattat ttgtttttcg caaaaaaaga gcaagacgag cactctatag agaggttcat
    19861 cttactcttt ttgtttattt tttatttcga cgttgagtag cttccataat gactggcaag
    19921 atcactgggc ggcgttttgt ttgttcaaat aagtaacggc ctaattgatc tcggatgtct
    19981 tgctttaatt tgctccactc aaaatcattg ctttctaaat gtttctcaac gattgtttca
    20041 atcatattgc tgctttcttt catcagatca cggctagctt ttacgtaaac gaatccccgt
    20101 gaagtaattt gaggttttga aatgattttc ttttctcgcc gactgatagt gacaaccgct
    20161 acgaaaatcc cgtcttctga caagattttt cgatcacgta aaacaatgtt tccgatatca
    20221 cctatgccta atccatcaat cattacattt tctgcacttg tagttcctgc aacactcata
    20281 cgcttgttct tatattccag cacgtctccg cgtccagtaa tatagatatt tttgtatggc
    20341 atccctactg catgagccaa atctgcatga gcagctaatt cacgatactc tccttgaact
    20401 ggcacaaaat atgttggttt catcaaattg atcattaatt gcaagtcatt aggattggca
    20461 tggccagaaa cgcgcatatt ctcagagatc aacttcactg ttccaccagc tcgataaatg
    20521 atatcttccg ttttcgccac gatggtttcc atcgcgatgc ttggcgtagt agtgatataa
    20581 accaaatctc cttcttcaat cttgacatta cggtgtgtac cgtttgccat tttttgcaga
    20641 gacttgatgg gttcacccat tcggccagtt tctaaaatga tcagttcttc tggatcatag
    20701 tttttcatgt ctttcaacgt aacaagcaga tcttcacttg gtaattgaag tttttctaaa
    20761 cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt
    20821 gctttttctg ctgcatccaa tacttgttgc actcgttgca aattacttgc tacacaagcg
    20881 acgatgatac gcccttccca atatttgatc gtatcaaata cttcatctgc tatttgggct
    20941 tcagaagcta caggctgagg attttctgca tttgccgatt cacttagtaa ggctaagact
    21001 ccttctttgc cgatttccgc caatcgtgca taatctgttt gatacattgg gatagccgtt
    21061 tggtcaaatt taaaatctcc cgtataaacg atactacctt cttttgtttt cagattgatc
    21121 cccactgaat ctggtatcgt atgagtggta cggaaaaagc tcactactgt ttcaccaaag
    21181 tctatttctg tatgctcatc taccacatgg aaatccttaa atcctttggc tgcttcatta
    21241 cgactgacat tcaattttgc taactcgatc gtcaactctg tcccgaatac tggtacagaa
    21301 atttttgaca acaagtaagg aagtgccccg atcgcatctg catgtccatg tgttaaaaag
    21361 acaccagcta cacgatcaat gttttcttct aaatacgtaa aatcagggat tacgacgtcg
    21421 atccccaaca actcattttc aggatatttt agcccacaat ctaaaataaa aatatcctcg
    21481 cccacttctg cgacatacat gttttttcca ttttcacgaa cgccgcctaa tgggataatt
    21541 ttaatggtac tcacaaactt cacctcttct tatgacatcc aagtttaaag atgtcgattt
    21601 tcttctttat ctaaatatat gagccattcc aatgaagcat atgcaaaatc actatttctc
    21661 aacttatgga gaatcagcag gaaaattacc agcacatccg cgttctgaca gttggatggg
    21721 cattttattt tggatatact tcattaacaa aatggatctt ttgcgaaacg ctatagccag
    21781 aatacttata taagtattca gctgttcccc attattatac aacaaataaa ctaaatcgct
    21841 agtatcagca attgttagaa caactatgca cgtaaaaatc cctctgtagc atctaggatt
    21901 tcttttgcta ttcctctttc atttcttctt cgtcggtttg aaacatactt ttttcatttt
    21961 accacatttt ttgcaataac tatagtgact ttttatatat acagaacctc atgttgatgg
    22021 tgtgcaaaca gcataagatt taagctagct ttaagatagg ttgtctatta tcaaatgcat
    22081 ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg catggaaaag tacaaaagta
    22141 aaatggggac gttcattgtt attgtttgcc gtgttcacag ccattcttat ttttgtgcta
    22201 gctggcctca cgattcgaag cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt
    22261 ggagcaacag tgactttaag tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact
    22321 tcttcaagcg gttctaccac tgaagaacgt ccagatccag gcagtttttc tcttacacca
    22381 gtttccttaa cggacgcgga aaaaatagct gaattagata atgtaaagag ttattccttt
    22441 gaaagttccg cctcagctat ggcaaaagat ggaataactg ccatcactag ttctgattca
    22501 agtgaagaca gttcagaaac tactgatgca ggcggtgaac ctgctggagg aatgggcggg
    22561 aatccgcaaa tgactcaagc agactttcaa atcagtggtg tgtcagcaac tgcacaaaat
    22621 agtaattttt ctgatggaac agcaaaaatc acggatggtg aaggaataga tgacagcgac
    22681 aaaggcacaa acaatgtagt cattgattct actctagcag aaacaaatga cctggcagtc
    22741 ggagatactt tcacaatcac gagtactgaa gatgaagata ccacttatga aatgacgatc
    22801 aaaggaattt atgaatccag cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg
    22861 aatccgtcga atacacttta cacttcttac acttttgcaa atgaactaaa tgggacatct
    22921 gacgataaca cgatagattc tgctatttac actctatctg atccagataa aatggatgag
    22981 tttctcaaag aagctgagaa gctgatcgac acagatacat ttagcttaca atcaaatgat
    23041 tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg caagtttttc taaaaatgtt
    23101 gttttactag tggctgtagc cggtattatc atacttactc tgatcattat gattaccatt
    23161 cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag gagaatccag aagtaaaatc
    23221 attctccaat tgtttaccga agtggctatt tgtatgattc ttgcattagg agttgcttcg
    23281 cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc tacttgacca acagacagaa
    23341 accacgacac aaaaccagca agctcctggc ggtggacaaa tgccaggtaa cagacaagaa
    23401 caaggcggtc aacgcgatgg tcaacaagga accacgaata atccatttgc tgtttctgat
    23461 caagtcagcg aattggaaat caatgtcaaa cctgcacaat tgggactatt agccggagta
    23521 gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta tagggatatt aagattaaac
    23581 ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt tacggacaga acaattggga
    23641 ttttggtatc aagatccgca agatgcgctt tataaagatg tagaactgaa tttcgaagct
    23701 ggaaaaatgt acgctatttt aggagcaagt ggttctggaa aaacgacttt tctttcgctt
    23761 atttctggac tagatgcacc aaaagaagga aaggtatact atcaagatcg ttcactgaac
    23821 aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga ttattttcca agcttacaac
    23881 ttgcttcctt atttatcggc tctagacaac gtcatagcag caatggaaat cactaagacc
    23941 aagcaagaga attatcgaca agttgcttta gatcgactgg ctcgtgtagg aattgatgaa
    24001 atattagcaa aaaaacaggt gaccaaactt tcgggaggtc agcagcagcg agtcgctatc
    24061 gttcgtgcta tctgctgtga acatcaacta atcgtagcgg atgagcctac tggaaatcta
    24121 gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat
    24181 cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt
    24241 gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat
    24301 aaaaaatccg aaccatatct gtctcgcaga tatggttcgg attttttatt tgcttgattc
    24361 aattagtaat aagtggtttt tatttttttt cgtcttttcg agaaaacttg tctttgactt
    24421 cgtcgcctaa atttcgagct ttttctttga tgtcagcacc tagtgattgt gctttccctt
    24481 ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc ttcttttcct tcaccgacta
    24541 ctttatcttt catatcttta gaacgtcctt ttaaatctgc cattgtgctt cctcctctta
    24601 gtttgtttat tgatctacct tcagggtaac atcaacaaca gatacagaca aacagaacgc
    24661 tcgttttcta aaaaagatag ttagagctgt tgattaaaaa cttttttcga aagtccccca
    24721 tactttgact tctttttctt ggaccattat ttctccttta gcgataactg tatcaatatc
    24781 aagcgtttct ttatccaaga tacacagatc tgcatcagca ccaatttcga tttttccttt
    24841 tttatccaat ttcaatatac gtgctggatt actggttata gctcgtaagg cgatttccaa
    24901 agggatactt tctttttgca cagcttcttt gatccctact agcaatgcct tggcacttcc
    24961 tactcctaac cctaggaaat gattgttttc atcaaaataa ggcaagcttc cttgtccatc
    25021 tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt
    25081 gctgaagcga acttcaccat ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa
    25141 gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg
    25201 atttgcatga gtaggcaaaa attgtgtgat cggaatttct gtttcttcta cgattcgtgt
    25261 caatagctct agctttcttt ttcctccgcc taagtggaca ttgatgatcc cagcttttcc
    25321 ggataacatg cctcctacac gagcatctgc tactgcacga gcgaactctt caaaacttgg
    25381 ctgagaagaa cggtgatcgg atactgcgat ttctccgatt ccgataattt tatcaattgt
    25441 caaaaagtct ttgatgatag aatctgttac tgtttttacc ggcaatcggt acgaaccttc
    25501 gtatacataa gtggagattc cttctgcttc cagacctttc gctttgctga tcaacgcagt
    25561 catatctcta ccttctccgt ctgttcccaa acaaccgaca actgtcgtta caccagctgt
    25621 agtcaattgg ctcaatgtga cttctggcgt tcgattttga taaccgcctt ctcctcctcc
    25681 acctaaaata tggaaatgac agtcaatgaa acccggggtt agtacttttc cctcacctga
    25741 aagttcttct acttccactc cttcgtagcc gccttctagc tgttcttcta cagccaaaat
    25801 cttcccaccg gcaatcagta catccatttt tcctaagtaa tctggtgcaa acacttcaat
    25861 ttgtcgaatg attttcatac atttttcctc tttctttaag tataactgat gataggatct
    25921 taaaaaataa tccaaacgat aggatcttta ctcctttgat tttaccgtaa aatgacggtt
    25981 cttggagtcc ttatctactg tttggattac ttttttattt acaggtctcc agtactactg
    26041 cagacctatt gcaatggcaa tcatgatgaa aatagttcct aaaacaaaca ggaatccttg
    26101 catcttgatt tggaatcttg cccattttgc ccattcgatc cgtgcaacac ctagcacacc
    26161 aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc
    26221 aagaactgct acttgtctgg atacacctac taaatctgcc agtggagcca tgattggcat
    26281 agtcaaagct gcttgtcctg aatttgatgt aacaaccaag ttgaacaaac tttggaagat
    26341 gtacatcgcc caagcaccaa tgacagctgg aacaccggtc aacgccgtcc cgatcccatg
    26401 caaaatcgta ttcaaagctg aaggaacaga tgcatcagat cctcccaaga caagaagaat
    26461 ccctttggcc atacctacta cgattgctgt accagctaaa tcagctgcac cagattggaa
    26521 agaagaagcg atgtcattga ctttcattcc atctaatttg aaaagaacag caatgattcc
    26581 agcaactaat cccatcacaa agaattgaga agcgatttct ggaatgtaat agcctttttg
    26641 agtcacgccc caaacgatcc agacaaggac aactaacatc tctaataaga tcaatttatg
    26701 cccaagcatg aattttttat tttcatcaga tgtctttttg atatgagaac ggaaataatc
    26761 atctgatttg taagtcaacg aactttctgg ttttttacgg attttttctg catatatcat
    26821 catataacca gcggctaatg cagtaacaac tacccacata atcaatcgga atgtcgcacc
    26881 agacaacacg gggattccag caatcccttg agccaccgct acgctaaacg ggctcatcca
    26941 agaggtcgca tttccgactt gagatgcgac ataagtcacg gttactgcta cgatcgagtc
    27001 atatcctaac gcaatgacaa atggaaccat caccattgag aatggaatta cctcttctgc
    27061 catcccgaat gtagcgccac cgaatgaaaa agcaaagaat aatagcggta aagctaaacg
    27121 ctccagtccc ttagttttac tgataaacgc atagatacct gcgtcgatcg ctccggtacg
    27181 cataataata ccaaaagcac ctccaacaac aaggatcaat gcagcgatcc ctacagcaga
    27241 tccatactta tctccagata ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc
    27301 aaaatcatct gttccccata ttttggctgt tttatgcaat tttttagaag tatcatagat
    27361 attttcgccg tactgatcgt aaagcacatc atctgtcaag gagatttcat ctagtttttc
    27421 ctgcgtaaga tttttttctc cgtcagcaat gactttttcc aatccttttt ccggaacatc
    27481 cagtttttca cgttctgctg gatgatcttg cagttcttct aattgatcaa acacatacga
    27541 cttatccagc tcgtaagcat aacggaagga atcttgacgt aaaacagtac gagtacttgt
    27601 ttcgccattt gcatctttgt attcaatatc ttgcgtgctg aactttcctg ctggaacgac
    27661 aaaagtcagt atccatgcaa agatgactac accaaaaata atgacatacg tatggggtgt
    27721 cgataattta gtgaagtcgc gtttcggttt tcctgaagaa tttgttttct tcaaatttat
    27781 ttcttccttt cttttttcag taattttgat gaataaaaaa tggataatca aatgaattat
    27841 acagtaaaaa tgacatttcc acatgaataa tttattagaa aatcactttt ttctattttg
    27901 t
//
LOCUS       DACDLI010000032        27596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDLI010000032 DACDLI010000000
VERSION     DACDLI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.032"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IV353_001424"
     CDS             162..392
                     /locus_tag="IV353_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9009681.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IV353_001425"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IV353_001425"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9009682.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IV353_001426"
     CDS             1803..1979
                     /locus_tag="IV353_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9009683.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IV353_001427"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IV353_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9009684.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2594..3061
                     /locus_tag="IV353_001428"
     CDS             2594..3061
                     /locus_tag="IV353_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9009685.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="IV353_001429"
     CDS             3146..4030
                     /locus_tag="IV353_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9009686.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4147..4380
                     /locus_tag="IV353_001430"
     CDS             4147..4380
                     /locus_tag="IV353_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9009687.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4468..4548
                     /locus_tag="IV353_001431"
     tRNA            4468..4548
                     /locus_tag="IV353_001431"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4502..4504,aa:Tyr,seq:gta)
     gene            4555..4626
                     /locus_tag="IV353_001432"
     tRNA            4555..4626
                     /locus_tag="IV353_001432"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Gln,seq:ttg)
     gene            5141..5848
                     /locus_tag="IV353_001433"
     CDS             5141..5848
                     /locus_tag="IV353_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9009688.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5908..6552)
                     /locus_tag="IV353_001434"
     CDS             complement(5908..6552)
                     /locus_tag="IV353_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9009689.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6679..6966
                     /locus_tag="IV353_001435"
     CDS             6679..6966
                     /locus_tag="IV353_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009690.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7074..7394
                     /locus_tag="IV353_001436"
     CDS             7074..7394
                     /locus_tag="IV353_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9009691.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7475..8788
                     /locus_tag="IV353_001437"
     CDS             7475..8788
                     /locus_tag="IV353_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9009692.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8785..9504
                     /locus_tag="IV353_001438"
     CDS             8785..9504
                     /locus_tag="IV353_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9009693.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9632..10183
                     /gene="raiA"
                     /locus_tag="IV353_001439"
     CDS             9632..10183
                     /gene="raiA"
                     /locus_tag="IV353_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9009694.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10405..12939
                     /gene="secA"
                     /locus_tag="IV353_001440"
     CDS             10405..12939
                     /gene="secA"
                     /locus_tag="IV353_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9009695.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13065..14166
                     /gene="prfB"
                     /locus_tag="IV353_001441"
     CDS             join(13065..13127,13129..14166)
                     /gene="prfB"
                     /locus_tag="IV353_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9009696.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14271..14957
                     /gene="ftsE"
                     /locus_tag="IV353_001442"
     CDS             14271..14957
                     /gene="ftsE"
                     /locus_tag="IV353_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9009697.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14941..15834
                     /locus_tag="IV353_001443"
     CDS             14941..15834
                     /locus_tag="IV353_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009698.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15986..17434
                     /gene="cls"
                     /locus_tag="IV353_001444"
     CDS             15986..17434
                     /gene="cls"
                     /locus_tag="IV353_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9009699.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17771..18628
                     /locus_tag="IV353_001445"
     CDS             17771..18628
                     /locus_tag="IV353_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9009700.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18743..19663
                     /gene="pstC"
                     /locus_tag="IV353_001446"
     CDS             18743..19663
                     /gene="pstC"
                     /locus_tag="IV353_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9009701.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19663..20547
                     /gene="pstA"
                     /locus_tag="IV353_001447"
     CDS             19663..20547
                     /gene="pstA"
                     /locus_tag="IV353_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9009702.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20562..21368
                     /gene="pstB"
                     /locus_tag="IV353_001448"
     CDS             20562..21368
                     /gene="pstB"
                     /locus_tag="IV353_001448"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9009703.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21384..22142
                     /locus_tag="IV353_001449"
     CDS             21384..22142
                     /locus_tag="IV353_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9009704.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22155..22832
                     /gene="phoU"
                     /locus_tag="IV353_001450"
     CDS             22155..22832
                     /gene="phoU"
                     /locus_tag="IV353_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9009705.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23070..24101
                     /locus_tag="IV353_001451"
     CDS             23070..24101
                     /locus_tag="IV353_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9009706.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24098..24865
                     /locus_tag="IV353_001452"
     CDS             24098..24865
                     /locus_tag="IV353_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9009707.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24965..26533
                     /gene="liaX"
                     /locus_tag="IV353_001453"
     CDS             24965..26533
                     /gene="liaX"
                     /locus_tag="IV353_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9009708.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26833..27150
                     /locus_tag="IV353_001454"
     CDS             26833..27150
                     /locus_tag="IV353_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9009709.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27150..27500
                     /locus_tag="IV353_001455"
     CDS             27150..27500
                     /locus_tag="IV353_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9009710.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         9108 a         4669 c         5959 g         7860 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat
     2461 tgccgaagtg ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac
     2521 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4501 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4561 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4621 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4681 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4741 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4801 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4861 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4921 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4981 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     5041 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     5101 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5161 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5221 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5281 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5341 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5401 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5461 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5521 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5581 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5641 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5701 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5761 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5821 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5881 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5941 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     6001 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     6061 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6121 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6181 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6241 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6301 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6361 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6421 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6481 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6541 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6601 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6661 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6721 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6781 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6841 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6901 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6961 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     7021 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     7081 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7141 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7201 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7261 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7321 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7381 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7441 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7501 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7561 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7621 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7681 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7741 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7801 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7861 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7921 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7981 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     8041 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     8101 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8161 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8221 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8281 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8341 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8401 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8461 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8521 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8581 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8641 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8701 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8761 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8821 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8881 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8941 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     9001 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     9061 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9121 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9181 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9241 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9301 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9361 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9421 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9481 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9541 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9601 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9661 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9721 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9781 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9841 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9901 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9961 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
    10021 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
    10081 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10141 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10201 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10261 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10321 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10381 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10441 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10501 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10561 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10621 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10681 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10741 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10801 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10861 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10921 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10981 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    11041 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    11101 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11161 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11221 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11281 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11341 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11401 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11461 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11521 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11581 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11641 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11701 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11761 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11821 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11881 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11941 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    12001 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    12061 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12121 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12181 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12241 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12301 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12361 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12421 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12481 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12541 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12601 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12661 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12721 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12781 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12841 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12901 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12961 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    13021 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    13081 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13141 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13201 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13261 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13321 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13381 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13441 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13501 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13561 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13621 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13681 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13741 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13801 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13861 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13921 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13981 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    14041 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    14101 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14161 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14221 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14281 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14341 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14401 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14461 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14521 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14581 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14641 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14701 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14761 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14821 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14881 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14941 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    15001 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    15061 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15121 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15181 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15241 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15301 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15361 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15421 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15481 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15541 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15601 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15661 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15721 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15781 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15841 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15901 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15961 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    16021 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    16081 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16141 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16201 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16261 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16321 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16381 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16441 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16501 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16561 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16621 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16681 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16741 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16801 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16861 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16921 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16981 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    17041 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    17101 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17161 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17221 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17281 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17341 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17401 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17461 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17521 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17581 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17641 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17701 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17761 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17821 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17881 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17941 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    18001 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    18061 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18121 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18181 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18241 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18301 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18361 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18421 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18481 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18541 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18601 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18661 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18721 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18781 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18841 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18901 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18961 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    19021 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    19081 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19141 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19201 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19261 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19321 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19381 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19441 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19501 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19561 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19621 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19681 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19741 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19801 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19861 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19921 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19981 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    20041 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    20101 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20161 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20221 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20281 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20341 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20401 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20461 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20521 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20581 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20641 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20701 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20761 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20821 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20881 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20941 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    21001 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    21061 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21121 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21181 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21241 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21301 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21361 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21421 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21481 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21541 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21601 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21661 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21721 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21781 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21841 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21901 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21961 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    22021 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    22081 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22141 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22201 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22261 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22321 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22381 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22441 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22501 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22561 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22621 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22681 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22741 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22801 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22861 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22921 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22981 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    23041 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    23101 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23161 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23221 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23281 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23341 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23401 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23461 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23521 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23581 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23641 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23701 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23761 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23821 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23881 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23941 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    24001 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    24061 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24121 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24181 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24241 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24301 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24361 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24421 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24481 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24541 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24601 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24661 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24721 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24781 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24841 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24901 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24961 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    25021 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    25081 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25141 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25201 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25261 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25321 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25381 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25441 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25501 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25561 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25621 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25681 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25741 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25801 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25861 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25921 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25981 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    26041 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    26101 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26161 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26221 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26281 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26341 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26401 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26461 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26521 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26581 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26641 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26701 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26761 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26821 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26881 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26941 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    27001 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    27061 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27121 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27181 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27241 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27301 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27361 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27421 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27481 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27541 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt ttttta
//
LOCUS       DACDLI010000033        26465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDLI010000033 DACDLI010000000
VERSION     DACDLI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.033"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            104..400
                     /locus_tag="IV353_001456"
     CDS             104..400
                     /locus_tag="IV353_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9009711.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1044..1997)
                     /locus_tag="IV353_001457"
     CDS             complement(1044..1997)
                     /locus_tag="IV353_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9009712.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2020..2775)
                     /locus_tag="IV353_001458"
     CDS             complement(2020..2775)
                     /locus_tag="IV353_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9009713.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2772..3731)
                     /locus_tag="IV353_001459"
     CDS             complement(2772..3731)
                     /locus_tag="IV353_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9009714.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3724..4674)
                     /locus_tag="IV353_001460"
     CDS             complement(3724..4674)
                     /locus_tag="IV353_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9009715.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4824..5276
                     /locus_tag="IV353_001461"
     CDS             4824..5276
                     /locus_tag="IV353_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9009716.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5365..7632)
                     /gene="gshAB"
                     /locus_tag="IV353_001462"
     CDS             complement(5365..7632)
                     /gene="gshAB"
                     /locus_tag="IV353_001462"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9009717.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7819..8817)
                     /gene="ftsY"
                     /locus_tag="IV353_001463"
     CDS             complement(7819..8817)
                     /gene="ftsY"
                     /locus_tag="IV353_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9009718.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8828..9637)
                     /locus_tag="IV353_001464"
     CDS             complement(8828..9637)
                     /locus_tag="IV353_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9009719.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9646..13227)
                     /gene="smc"
                     /locus_tag="IV353_001465"
     CDS             complement(9646..13227)
                     /gene="smc"
                     /locus_tag="IV353_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9009720.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13247..13933)
                     /locus_tag="IV353_001466"
     CDS             complement(13247..13933)
                     /locus_tag="IV353_001466"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9009721.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14281..16062)
                     /locus_tag="IV353_001467"
     CDS             complement(14281..16062)
                     /locus_tag="IV353_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9009722.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16088..17002)
                     /locus_tag="IV353_001468"
     CDS             complement(16088..17002)
                     /locus_tag="IV353_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009723.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(17029..17991)
                     /locus_tag="IV353_001469"
     CDS             complement(17029..17991)
                     /locus_tag="IV353_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009724.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17991..18935)
                     /locus_tag="IV353_001470"
     CDS             complement(17991..18935)
                     /locus_tag="IV353_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009725.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18936..19940)
                     /locus_tag="IV353_001471"
     CDS             complement(18936..19940)
                     /locus_tag="IV353_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009726.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20208..20450)
                     /gene="acpP"
                     /locus_tag="IV353_001472"
     CDS             complement(20208..20450)
                     /gene="acpP"
                     /locus_tag="IV353_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9009727.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20572..21573)
                     /gene="plsX"
                     /locus_tag="IV353_001473"
     CDS             complement(20572..21573)
                     /gene="plsX"
                     /locus_tag="IV353_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9009728.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(21622..23658)
                     /gene="recG"
                     /locus_tag="IV353_001474"
     CDS             complement(21622..23658)
                     /gene="recG"
                     /locus_tag="IV353_001474"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9009729.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23775..25457)
                     /locus_tag="IV353_001475"
     CDS             complement(23775..25457)
                     /locus_tag="IV353_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9009730.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(25509..25871)
                     /locus_tag="IV353_001476"
     CDS             complement(25509..25871)
                     /locus_tag="IV353_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9009731.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            26141..26329
                     /locus_tag="IV353_001477"
     CDS             26141..26329
                     /locus_tag="IV353_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9009732.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7295 a         5737 c         4668 g         8765 t
ORIGIN      
        1 tttaacaact attggaaaaa gaattacttt ttgttcacgg attattaaca actcttcggt
       61 agagtaaact tatcaaataa aagacttctt tggaggaatc actatgaagg aatatataaa
      121 aaacatctat tttatcgaag aaacacaaaa tatcgagggt tcttatattg aagtaaaaac
      181 actctttgtc aatgaagata aaaccaaagc attggatatt tataaaaaat tagcttcaaa
      241 aaaaacgaat agttttggat tgatattaag cgagtataaa atcaaagcag aagagagtta
      301 tttctatcaa ttattaaaac ggtggagtaa gcttcccgcc gatttttatc gaaaaatgca
      361 gatcatcaac tatcaaccgc ttgctgagac acatgcctaa atagaggttg tgaaatcagt
      421 gctttgagct aataccgaag gtcagctgat tgaacattgt ttagaagaga ttgtgacaac
      481 tgcgatttgc tttgagtaat aagaacgctg gacagaaaat agcttctcat attttctagt
      541 ctagtgggct tattgccgag aagcagcaga tggaacatcg tttagaagag attgtgacaa
      601 ctgcgatttg ctttgagtaa taagaacgct ggacagaaaa tagcttctca tattttctag
      661 tctagtggac ttattgccga gaagcagcag atggaacatc gtttaaagaa gagatcggta
      721 caaaataaaa atatctaaat ttaagtttta tctaagtttc gctttgtacc tttgattcaa
      781 atcaaaggag gcgatcttga tgctgaaaaa atggatcaca ctattttttg ccactcccca
      841 ttctagaatc aacccgtatg atgtagataa tgaattcacg ttgaaaaaag atttctcaaa
      901 taaataaaaa ccatccgtaa aatcatcaat acgattttac ggatggtttt tgataacttc
      961 tttgaggata ggctcttgct atttaagaat gtaagaaaca ttattgttac atagtatgag
     1021 agtttaatcc tctgttattt atgctattta aatgcttgat tcacatcttc gatcatcagt
     1081 ttcattgatt ctaaaccgcc cccgcttaaa taccagacat ctggttcaag ggagatgatc
     1141 tgctgatttt taccagcatt cgtttgtgct accagttcat tggcagaaat atcatctttg
     1201 ctgtcgtctc caccaatagc ttttgttctg tctactacaa ataagatatc cggatttttt
     1261 tccagcacat attcataaga aacactttga ccgtgagtgg atgcttcgat ttgatcatct
     1321 gcttgttcaa agccaaataa atcatggatg aaaccgaatc tagaaccaga tccgtaggct
     1381 gataattgcc cttcattcac aagtgtgact agtgcttttt tgtcagtagc ttctgctttc
     1441 tttttcgtct gatcgattgc actagataat tcctctaatt gagttttagc ttcttcttgc
     1501 ttaccaaaga tctcccctaa agcagtgaca ttttgctgga aagattccca tggatttttc
     1561 gcatctaaag aaaggaaaat ggtcggagca atttgactta gatctttttg aaaatctctt
     1621 tgtcggccag aaatgatgat tagatctggc tgaagctgat taattttttc taagtctggt
     1681 tcttttattc cacctgctga ttctactttt ttataggatg aaagatactc aggaaggctg
     1741 cttgtagctg ctccaactac cttatctcca acaccaagag catccatcgt atctaaagaa
     1801 ccattgtcga atacgacgac tttttctgga ttatttggca cctcgatttg gtctccatta
     1861 ctatctgtta tcgtcaatgt agtttcagtt tgtgtgctgc ttttttttgt gtctgcctga
     1921 ctggtgttgt tgctatttcc acatgctgct aaaccaaatg atgctagaat caagatacct
     1981 actactaatt ttttcatctc ttctctccta atgttagttt tagaaataaa gacagaatct
     2041 gcggccttca atctcacaaa tccgtatgtc catttcgtat aattcatcca atacatcttt
     2101 ttttatcatt tcttgggtcg ttccttgcgt aaataactgt ccatctttca ttgcaacaat
     2161 ttcgtctgca taacttgcag caaagttgat atcatgaaga acaataacaa ttgttttccc
     2221 gatttcttgt actaaatttt ttaaaatctg catgatttga accgcaaaat tcatatctag
     2281 attgttcaaa ggttcatcca gtaaaatata atccgtgtct tgcgctagca ccattgcaat
     2341 atacgcacgt tggagttgac cgcctgaaag agtgtcaatc gattgatccg ctatttcttc
     2401 taacccaagg tgttccagtg attcctgaac gatttcctcg tcttttgtcg tcaattttcc
     2461 tttcgtataa ggaaatcgtc caaattctac taattctttc acggtgattt ggacatttat
     2521 ttcattcgat tgtcgtagga tcgataattt tttggctaat tcattggatt tccaagcctt
     2581 tacttcgtta tggtctaaat agatttcacc tgtatctttt ggtatcaaac gactgatcat
     2641 cgacaacacc gttgactttc cagcaccatt tggaccgata aaagcagtca atttcttttc
     2701 aggaatcgtt aactgtacat ctttaaccac tgattttcca tggtacagtt tattgacctt
     2761 ttgtaaatca atcataggat actcttcctt tcgcgaatca attttgtgac aaaataaatt
     2821 cctccgacaa attcaacgac gacactcact gttgtattcc agccgaaaat ctgctctact
     2881 gcgagctgtc ctcctgtcag aaacagcata gctaccagaa agcttccgat aaatagctga
     2941 ccatgacgat aagtagaaaa gacacgataa ctgatatttg cgacaataaa ccctaaaaaa
     3001 gtaactggac ccacaagagc cgttgatgtc cctgtcaaaa gactgacaca acataaaatc
     3061 aaaaattcaa aacgatctgt gtctatcccg aggtttactg cttgttcatt tccgagatgg
     3121 aaaacgtcta gcacatgtcg atatcgaaat aacaggtacc agcagccgat aatgatcaaa
     3181 gaagcaggca aaagatactg actctggata ttgccaaagc ttgcaaataa tctgccttga
     3241 agcaaatcat attcattcgg atccatgatg acttgtaaaa aggtactgac actaccaaaa
     3301 agcgtaccta aaatcatccc tgacatcagt aataaaaaaa gattcgctct catttttttc
     3361 aaaagaaaga aaaataacaa tagactcagc ccgaccatca gcccaacgtt caataagaaa
     3421 aaaactgtac ctgtctgctg aagcatcgtc actcctccga taaagaagaa aaggaccgtt
     3481 tggacaagga catataacga ctcaagtccc aatactgaag gagtcaaaaa ctggttatgc
     3541 gtaattgtct gaaagctgat tgtggcactg ctgacagcta gcgagacaag gataaatgca
     3601 aaaattttct tactcctcaa tgcacatgca aaatgccagt ttccatatgt atggatcgtc
     3661 aagtacattc ctgtaacaaa taaaacaaga acaagcatac caataaaaat agatggcttt
     3721 tttttaagca tatcgtcgtc cccttatcag aagataaagg aagatagccc caccgatgat
     3781 tccaacgatc acactgactg gtatttcata aggaaaaatc aacacgcgag cgacaagatc
     3841 gcaaaatact aagaacaaac tacctgctaa agcagttacg ccaattgttt tgtgcacttg
     3901 atctccatat attctagaga caagattcgg aatgatcact ccgataaaag gaatgcttcc
     3961 taccatgatc aacatgactg cactagccaa tgatactaag ccaatgccta aaaactgcat
     4021 cttctggtaa tctattccca gatttactgc aaacgattcg cccagaccaa tgatcgtaaa
     4081 tcggtaagca aatagccaaa ccaagagcaa caatggaaca acagcataca ataactcata
     4141 atttcctcgt atcacagtcg caaaattccc ttgaagccat gaagtcatat tttgtaccaa
     4201 ctggaattga taagcaaaaa aagtagcaac tgaaccaatg atattcccta acatgactcc
     4261 aatcaacgga agcatcaatt gatttttctg gaggaacaat cgcgtaaggg ataaaaaaag
     4321 caatgttcct gcaaaagcaa agcaaaatgc tgtcaaggat cggacaatca aggatgcacc
     4381 aggtaggaaa atcatagcca ctaaaatacc taaacgcgca ctatccatcg tgcccgcagt
     4441 cgttggtgaa acaaatttat tttgggtcaa atgttgcatg atcaacccac aaatactact
     4501 ggtcgctccc acaaggatca aacaaatcgt tcgtggcaaa cgagtcacta gcaagatttg
     4561 taagctttgc ccatctaact ggagcagttc tgacaattgt acattcgata ctccgatgaa
     4621 caatgaaata ctacttaaaa taactaataa tgctactagt cgtccgtagc gcatccaatc
     4681 cacctttcta attgagaatg attttcattc ttattttaag tgaatcgttg aacaaaagca
     4741 accgcttttt catatgtttt aggtattatt gggaatatgc tataatcgta agaaagacaa
     4801 agagaagaag gagggtattt tttatggcac aagatgatgt aaaaagccat ttggataagg
     4861 gactttatgg tacaccttta gtgaaccctg aagaacagca taaatattta gggacattca
     4921 gggaacgctg ctatcttagt atgactgttg ctgaaatgat ccaaccaaaa aacaaagaaa
     4981 actttttaaa agaactcaaa aaacatccag atgcttccat actcttgaat ggggctctac
     5041 ccctttctgt ccaaaaccaa tatatccagc tagcaacgaa aaacaatagc cgtttcactg
     5101 tagtcaacga ttacgtcact gcaaatcctg aggaattcgg attgctctta acagcaaaag
     5161 aagcagtaaa tgaacctgtg attgatatcg aaaaaaaata cccagaagaa aactcttcgc
     5221 cagctgaaaa agaaaaacca aaaaagaaaa aaagcttctg ggatcgttta ttttaaaacg
     5281 aaaaaacaca taaattttta catgcaaaaa aagcttttga tcagattgac atcaaatagg
     5341 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
     5401 catcgtcaat cttctgcctt ttcctgcaaa aggataaaca tgcatatgca tggctggatt
     5461 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
     5521 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
     5581 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
     5641 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
     5701 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
     5761 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
     5821 tgttcggata ctgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
     5881 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
     5941 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
     6001 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
     6061 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
     6121 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
     6181 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
     6241 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     6301 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     6361 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     6421 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     6481 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     6541 aaccagcatt tgcttcattc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     6601 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     6661 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     6721 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     6781 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     6841 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     6901 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     6961 cagcgcactg ttgcgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     7021 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     7081 aaagtttctc gctgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     7141 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatgaat
     7201 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     7261 atcttcgaaa ttttttaatt ttgcaataat gatctcttcg tctttttccg gtaaggcagg
     7321 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     7381 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacagggg tgatcatctc
     7441 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     7501 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     7561 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     7621 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     7681 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     7741 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     7801 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     7861 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     7921 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     7981 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     8041 cgcgttttga ccagtggttg catctaatac aagaagtgtt tcttggggtg cttctggtaa
     8101 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     8161 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctgtctttg ctctttccaa
     8221 agcatcgaat acgacagctg ctggatcgcc tcctgcattg ccacgaacaa cttcaacacc
     8281 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     8341 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     8401 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     8461 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     8521 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     8581 tgcttcggta attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     8641 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     8701 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     8761 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     8821 actcccccta ctctaataca aatttttgga tcactttggc tacgccattt tcttcattgc
     8881 tggctgtcac gaaatctgct gcttctttga cgaaaggcac agcattctcc atcgctacgc
     8941 ctagtccagc atattggatc atcggtaaat cattttcctc atcaccgatc gccatgattt
     9001 cttcttgttt caaaccaaga tcttttgcca ataaagaaat accataagct tttgtaacac
     9061 cttttggcat aaattccaaa agcatacttc ttgttttgat gatctcatac atttcatgga
     9121 attctgctgg tatttctttg attttaggat ctaattcttc ttggtcataa gcgattactg
     9181 ccttattaag cacaaattca tctgtaatat cgcttatatc cgctggttgg aattgcagca
     9241 gcttattcaa cacattgtac aaggattgct tccttggggc actaggcagc tgtaaaacca
     9301 cattgtcaga caaaacatct agtggcagat ccaattgctg agccagtttg tataagcgtt
     9361 ggatatctga tacttccatc actttctttt ccatgatttc accagtatca tttttttgta
     9421 ccaaaccgcc gttgaaagtg atgctgtagt caccttcatc taccagtcct aattcttgca
     9481 gataatgcgc catcgcagct aaggggcgtc ctgtacatag aacgatcttg accccttgct
     9541 gttttgcttg tgctaatgct tgtttattct catctgaaat cagcttttgt tcatttaata
     9601 atgtaccgtc caaatcgatt gctactaatt tgatcatttt cgttcctaca ctttcgtttc
     9661 gttgattttc gcctcttcat tgatatcttc catccgtaca gaaacgattt ttgatacacc
     9721 ggattcctgc atcgtcactc cgtacagtac atctgctgcg accatcgttc ctttgcggtg
     9781 tgtgactacg ataaattgtg tgtcatcttg gaaatcgctc aagtaacgac caaaacgttt
     9841 cacgttggct tcatctaacg ctgcttctac ctcatctaag atacaaaacg gaacaggaca
     9901 gacacggata atggaaaaga gcaacgcgat ggctgtaagt gctcgttctc ctccagaaag
     9961 caaacttaga ctttgcagtt ttttgccagg aggttgtact tcaatctcaa tacctgtttt
    10021 cagcaaatca gttggttccg tcagtactaa ttcagcttta ccgccgccaa acatatttgg
    10081 aaagacaatt ttgaattctt gacggatagc ttcaaatact tctttgaatc ttgtccgcac
    10141 ttcatcatcc atctcatcca tcgtttcaaa caattggttt ttcgctgcta acagatcatc
    10201 tcgctgtgtt gctaagaatg tatgtctttc actgacttgt tcgtattgtt cgattgaatt
    10261 taagtttact ggtcccagtt tttcaatctg ttcttttaag gatgaaacct ttgttctact
    10321 gtcttcgata tcggttgttt cttgatagtc ttgggaagct ttctcaaatg tcagctgata
    10381 ttctgactgc agataggtca aatgattatc taaaatcatt tcagctcggt ctttttggac
    10441 ttctaatttg gattgttccg acagtcgtgc tttttgctca cgattacgct ctgccaagac
    10501 tgcttcaagc ttatcgatct ccttttgcct tctatctcgc tgctctttgg cttttgccaa
    10561 ctcggctttt aatgtttctc gctgagcaga taattcgtta atttgttttt ccaaactttc
    10621 ttctgtgact tcatgatcag aaaagtctgc tgtcaatgtc gccaactgct tttctatcgc
    10681 ttcttgacgt tcggttgcct ctgctttttg gactcgggca ccgcggagct ggatctgcag
    10741 atgattaaac tgctctttca agacagcaag atctgcttgt tcttgtgctt tttgactttg
    10801 tacttgtgct cttctggctt ccatctggtc gctttcttga cttaacgatt tgatttcttc
    10861 gtcgattttt tgtcgttgcg attcaagatc tgtttgtttg tctttcaatt cgttcgtttg
    10921 cttttcgtaa tcttcgataa actgctgcag ttcacgtgtc tcaaaagttg agatttgctt
    10981 ttctttttca aaacgttcaa gttcatttat gatgttttgt agctgatttg tcgcttcttg
    11041 ttcttcaaaa cgtaattgtt cgcctcgtgt acgcaaaact tcttgttctt cggctaaacg
    11101 agctgtttct tgttgcagct cctgaacttt tttttcttgt gcttggagtt gtttatctgc
    11161 ttcttcaaat tcactagtca actgtttcaa ttcttgattt tgtacaaaca aactgccttg
    11221 gtttcctcgt ttattcgctc cacctgtcat ggatcctccg gcattcatca catcaccttc
    11281 taaggaaacc actcgatatt gatagcggat cgtttgcgcg attgcattgg cactagttaa
    11341 gtctttggct aaaaggatcg ttcctagcag gttttgaacc acagtttgga tttgatcggg
    11401 ataactgact tgttcactgg caatccctat aaacccttct acagctgctg cttgtgtcaa
    11461 tatatgagcc ggtaattgtc ttggtttgat tgtcgtcagt ggaaggaatg ttgctcttcc
    11521 tcctctttgc tgtttcaaat acgtgatggc ttgccgagca tctttttcat tttcaacgat
    11581 gacatgctgg gctgccccac ctaaagctgt ttcgatagct aacgtgaaat ctgcaggaac
    11641 atcgatcagt tcagccactg ctccgacaat acccgaaagc tgctgtttat gctgcaagac
    11701 caatcgaacc ccttggtaaa aaccaaaata gttttcttgg atttcttgaa ggcttttctt
    11761 acgagcacgt agctgctgga cttgattcat taattgatac attttaggct gttcttttgc
    11821 taatgcttct tggaactttt tcccgttttg ttggatcatt tctaacttct cttttgtttc
    11881 agttaaagat gattttagct gcgcctgctt ttgtgtcaat tcttcttttt gtaagatcaa
    11941 tcggtctact gatgcttcta cttccgattg cttggctaac gtctgcttgc tttttgctgt
    12001 ttcttgtata tattgacgtt ctagatactt cagttcgttt cctacagctg cttgttcttg
    12061 catcaaatca acatattgat ctcttaattc tgccaataat tctttcgttg atttttggta
    12121 tttttctaat tcttgctgtg tttttatgag atttgcttca gctttttgga tctctgtttc
    12181 tttttctgct gctgctttca tcaagctttc ttgaagcttt tcaaaatgtt taaccttttt
    12241 ttgtgcttct gctaatgaag tttgatattc ttgcgagctt ttttgtgtat gctttgtccg
    12301 ctcctgcaaa acatcttttt gtccttctgt ctgcttcagt ttttccgaaa gatctaataa
    12361 tacttgctga tttttttcga tcagacgatc ggcttgcgca ttttctttac gctgcttcgc
    12421 caaaatggat tcttgttctt ggatcgactc agatagtttg cctaattcta gattgaattt
    12481 cgcaagctgt gcttgtttgt tgtcccaatc tttttttgct gtcttgattt cagcaaccat
    12541 caaagagaca tcggtttgag tcagtgtttc ttttagccgt aaaaactcct ttgctgcctc
    12601 actctgggcg gccaaaggag ttaactgttc ttccaattcg tggatgatgt cttggacgcg
    12661 gctgaggttg tcttctgttt caaaaagctt ctgttctgct ttctttttcc gctgcttgta
    12721 tttcaagaca ccagctgctt cttcaaagat cccacgacgg tcttccggtt tactactgaa
    12781 aatcgcttct acttttcctt gagaaatgat tgagaacgat tcttttccta atccagaatc
    12841 taagaacaat tcttggatat cttttaatct gcagctttgt ttattgatga aaaactcgct
    12901 ttccccagtc cgtcgataac ggcgggttac actgatctca ttgtactcca atggcagata
    12961 atgatcacta ttgtctaaaa tcactgtgac ttctgcaata ttcagctgct tacgggtatc
    13021 ggaaccggca aagatgatat ccggcatttt tccgcctcgc agacttttag cagattgttc
    13081 tcctaatacc cagcggatag cctctgtaat attgcttttt ccgctgccat ttggtccaac
    13141 cactgctgtg acgctatttt caaaatcaat gactgtcttg tctgcaaatg atttaaaacc
    13201 ggctatttca attcgtttca aatacaaagc tttccctcac ttacttttac agtgctgcca
    13261 atgcattttc cgccgcagct tgttctgcta attttttcga tttcccttgt ccagtgccaa
    13321 tcaattgatc atctacataa acttcgatcc aaaatactcg ttcatgagct ggaccttctt
    13381 cattgatcaa tcggtattcg attgatacat cacctgattt ttgcaagact tcttgtagtt
    13441 ttgttttatg atccatctca tgtgaaaaag cacctgcttt gactttagga aaaatcactt
    13501 tttcgatgaa tgcatgggtc gtgtcgaatc cctggtcaag atataacgcg cctaaaaaag
    13561 cttcgaatag gtcgcaaagc aatgctggtc gcgtacgtcc tcctgaattc tcttctccct
    13621 tgcccaacaa aacatattga tcaaagtgac attctttggc aaatttcgac aagctgtctt
    13681 cgcgaacgat cgcagctcgt gtttttgtca attttccttc tggcaaatga ggatataaac
    13741 ggaataaata ctgagaaacc atcagttcta aaacggcatc tcctaaaaat tcaagtcgct
    13801 cattatccga taattgcaga ttgcgatgct cattcacata ggatgaatgg gtaaaagctt
    13861 gttcaagcaa atttagatcg tgaaaaacga tgttatagtt ttcttttagt tcttttgtca
    13921 gctggttgtc cattttccac attcctttta tggatatttt ttcgatactt ccttattata
    13981 ctggtttttt gtcaaatttg aaatggttcg gtcaaaaggt aagtaaagct ttaataatcc
    14041 tagtgattga cgagaagttg tgaaaaattc gtttagtttc gagtatgaag aaaaaatttt
    14101 gtgaaaatgg ctttccatat ttttgcataa atttgactta ataccgagaa actggacttt
    14161 gaacaccgtt tattggagaa gtcatgaaat cagcgccttg acctgaatat tagagaaata
    14221 aacagaaaaa tatcaaaaaa ccgaaaaagg acatcgccct ttttcggtca tttttagtga
    14281 ttatgaacgt gaatctgctg taacagaaac atttgcccat gggtttggta catcataaga
    14341 ccaatcccag tctttgacac ggtcgttgac tggcaatact tcattacgga acaacgttgg
    14401 aatcgcaaat gcttcttcaa atgcatattg ttgccatttt tgatatgctt ctttttggta
    14461 ctcttcatca aatgctttgc ttgaactgat atcgttgatc aattttgtat tttcttctga
    14521 ttcataacga gtgtagttga acgcagcatt tggtccccat agtccacttg gatttggatc
    14581 agttcctgta ttccaagcag cttggtaaac atcaatttca ggatcatcgt tttctaattt
    14641 atcatagaaa gaattgaatt cgatcagacg acctgttgtg tattggacat ccaaaccaat
    14701 ttctttccat tgttggatat agtaatctgc caatggttgc gctgtttccc caccggacat
    14761 cgaagcaaag ttgattgtta gtttatttcc atctttgtct tctcttaaac catcgtcatc
    14821 cacatctttg tagccagcat catctaatag ttttttcgct ttgtctagat cttgtgtgaa
    14881 tcctttcatc tcatcatcat ggtatgactg gaatactgga ggaatcaatg aagtcgcatt
    14941 gcttctcaaa cctgcataga aacgttcacc tactgcatta ttatctaatg catagcccat
    15001 tgcttgacgt aatgatttat cagccatttt tgaatctgga ttgtattcca cactctttgt
    15061 atctgcattc catttaccta acttgaatcc tagataagta taagaagttt gttcacggcc
    15121 taagtttgta tagccagcaa tttctttata ggtatcgtaa gtatctgttg gcatttggaa
    15181 aatcatatca tataatttag agttcaatgc ttcagttgca cttgccatcg gtacagattt
    15241 catcgtgatc tttttcaatt taggcgtacc attccagtag tgctcgtttg gcaagtattc
    15301 tactgattct cctgcaacga tattactcat gtaataaggg ccaaaagtca ctggattttt
    15361 acgtacttga tcgcttgatt ccatatcttt gattgctaca ccttctaacg tatgttttgg
    15421 tgctgcatat gaccagatac caccacccga ttgaagcatg cttgggttta cttctttata
    15481 ttcgatttca actgttttgt cgtctatttt tttgattccg gagattgtgt cggcacttcc
    15541 atcatgatat tcttccatac ctacgatgtt acggaaagtg tcgtcataac ggataccagt
    15601 gtaatcttta ttcccaatga tttcgtaagg gaaaatcaca tcgtccgccg ttacatcttc
    15661 accatctgac catttgacgt tgtcttttac tttgattgtc gcttttttcg tatcttcatc
    15721 tagatcaagc gttgcagctc cgtcgtctgt gattctgaaa tcgtcatcgt ttgcaaatag
    15781 cggttcatga gaaggagcca taaaagctgc atcatatgaa tcttggtaaa actcccactg
    15841 gaacaatcct ttaaactgac tgtccgttgc gaccgcagct tctaattcgc cgtcttcgat
    15901 tgcatcatca ttattttttg ttgcaattgg aaaagttaat tcctctttgt tattgccgtt
    15961 gcctccagag ttgtttccag aggtatctgt acctccgccc ccgcatgctg caagaacgac
    16021 agcagacatc agcgctgcaa atccaaaaat cgttttcttc attcctattt cccccttcgc
    16081 ttctatacta gcctaaccgt tggcgcgcat ctgcagaacg cttgaatgct tgtccaacat
    16141 aattgatact caacatcaaa actaaaataa ggattgatgc gggtaaccag atccattgcc
    16201 gattcactaa gacttctccg ttacttgcat aaccgatcaa tgtacctaaa ctaggtacat
    16261 ttgccggcaa gccgaaacct aagaaagtca aagttgtctc gataccgatg tttgccgcaa
    16321 agttcatcgt caaattagta atgattaatg aacttagatt cggcatgatt tcgcggaaca
    16381 tgattttaaa atcattggtt cccattgttt ttgaggcact gacataatct cttcttgctt
    16441 cagataatgt cttgcttcgg aataatcggg ctttagctac ccaataaaat gcactcatga
    16501 tgaatataaa ggaccagaca ttgtatctgg aaataatcga tacgaagacg ataatgatca
    16561 gcatgatcgg tagaatcatg ataaaatcaa cgatccgcat caagatgttg tcaatgatcc
    16621 ctccgtagta gccagagatg atccctaatc caaccccgat gatcgaagta aggatcgtga
    16681 tcgcaaatcc gatcgtaaca gagtttctag cacctatgat caattgtcct aatacatcac
    16741 gtccgccttc atctgctccc agaatgaact tttgtccttg tgtcgaaact gttcctggtg
    16801 cagcgtattt gtcgaagata ctaacataca tcactttgtc ttgatcggtc agcatcgcac
    16861 cgataaagat cactaataat aaaaccacta gcaaacctaa tgaaaaaata gcaattttat
    16921 cttttttaaa ttctcgagca atcattcgta tccccatagg aggaatactt tcttgaactg
    16981 ctgtattgtt tttttcactc atcgatccat tctcctttct gattggtttt attgaatccg
    17041 aatacgcggg tcaacgatac tcatgatgat atctgaaagt aatgtcccca ctagcgtcgc
    17101 gattcctaaa atcaatacta aggatgtgat cacggtatag tcccgttggc cgatggaatc
    17161 aataaatagt tttccgactc ccggataacc aaaaatcttt tcgataacta cagaaccagc
    17221 aatcaacgaa gtgatttcat accccatttg agaagccatc ggcaacgctg catttcggaa
    17281 aatatgtcga ctgaatactt tattcgtcgg tacccctttg gaacgggctg ttctgacaaa
    17341 atcgagcgat ttcgaatcga ttacttcact tcgcagatat tggattgtga cggcagtcgc
    17401 caaaaatgct tgggtaattg atggtaacag aatatatttc aaacggtcta tccattgagt
    17461 gataaatcca ccttgcaaac ctagtgtttg tgatccactt gttgggaacc agcctaaacg
    17521 ataaccaaaa acaaatagca tgatcaagcc gaagataaat aaaggaacag caaaactaaa
    17581 gaagttgtag attacgacaa tcttatccaa gattgaattt tgataccggc cagcaagcat
    17641 ccccagaggc acagcaatca aataagtaag gacaactgtc aagatcgata gatataaagt
    17701 gttgatgatc cgttcgccga tcaagtcagc aacagagcgt ttgtataaga aactttgacc
    17761 aaaatcgcct tgcaaagcat ttccgatcca acggaagtac tggacatacc atgggtcatt
    17821 caagccagct gcttgtcgca tctgttcgat cactgccgga tcctgatttg gattgatcaa
    17881 accggtaaat ggatcccccg gcatcgcttg ggctaataag aagatcagaa cacttaaaat
    17941 aataacttgc gggatcatca ataaaacacg tcgcagaatc gttttccaca ttagcttgca
    18001 cctccatctt tcaacgccac tttgtgtgta gttgtgatcg tacgcaaatc gtagactcga
    18061 ccagtcgcat catagtattc tttttggttt tggatatact cttgttctac tcttcgtcga
    18121 ttttctttgt ggctttccct attttctaca tcgatttgcg gaatagctga aagcagacgt
    18181 ttcgtataga tatgtcgcgg gtcattataa atgtcttctc ttgtaccgat ttctacaaat
    18241 cgacctttgt acataattgc gatattgtcg cacatgtgtt ttactacacc taaatcatgt
    18301 gaaatgaata aatagctgag gccgtattct tgctggattc gtttcatgaa attcaatact
    18361 tgcgcttgaa cagatagatc cagagcagat acaggttcat cggctacgat cagttttgga
    18421 ttggtagcaa cagcacgggc tactcctaga cgttgacgtt gtccgcctga aaattcatga
    18481 ggatatttat atagcgcatc ttccggcatc ccaacgatat ctagcagtcc ttttaccttt
    18541 ttcttttctt cttgatcgct taaccgttca aaatttcgga tgggttcagc aatgatatcc
    18601 aacactcgct ttttaggatt caaactggac attgaatctt ggaagatcat ctgcacatcc
    18661 ttgttataac ccattttttt gcggttgctt cgcttcgtta catcttttcc ttcatacatg
    18721 atttgtccgg ctgttacctt ttctaatccc acaacggctt ttcccgtagt agattttcca
    18781 gagccagact ctcccactaa accatatgtc tttccttttt cgataataaa atccacacca
    18841 tctacagcat atacatgatc cgttactcga ttgaagaatc cactgcgaat agggtaatga
    18901 actttcaggt ctttgattgt gattaattct gtcatctaca cctctccttt ttcgtctcta
    18961 aagtggaaat gcttatagca ggagcaacgt accaagtggt tcggtgcgac ttcatgaagg
    19021 ataggctctt cttcatgatc gcttgctggt atccatggaa tacgtggtgc aaaccgacaa
    19081 cctttacgag gcatgttttt cagagaagga acgacacctt cgatcacatg gagttctgca
    19141 tcatgatttc cttcttgtgg tatagagttc agtaaagagc gtgtataagg atgcttagga
    19201 tgatcaaaca gttcttttac acctgctact tcaacgaact gtccgccata catcactgcc
    19261 actcgatcag ccatttctgc tacgactcct aagtcatgag tgatcaagat gatccctgtt
    19321 tttgtttctt cctgaaggtc tttcagtaaa tcaagaatct gtgcttggat ggttacatct
    19381 agggctgtcg tcggttcatc tgcaatgacg attggaggtt tgcaggcgat tgcgattgcg
    19441 atgatgacac gttgtctcat accgcctgat agttcatggg gatattggcg tccaactctc
    19501 gcaggattag gaatccctac ttgttctagc aattctagtg cacgagcttg acgctgttct
    19561 gcattcatct ttgtgtgata ggaaagtcct tctttgatct ggtcttcgat tctcatgaga
    19621 ggatttaatg cagacaacgg atcttggaaa atcattccga tatcatttcc gcggatttta
    19681 ttatagagcg tttcgttcag gtcgattaaa ttcagatcgt tatataaaat ctcacctgta
    19741 atttttgtgt tattaggatc aagtaatccc ataatcgtag tagccaaggt acttttcccg
    19801 cacccagatt ccccaacgat tgctaaaatc tcgtttttgt ctaaagtaaa tgaaacgtca
    19861 tctactgcat cgtaatagtc atctttgagt cggaatcctg tatgtaggtt ctgaacttct
    19921 aataattggt tatcggacaa tcgaataccc ctctctgctt cctaaatgga agtttttctt
    19981 aataaaaaag gacattacac agaataaatc gttttataac aaaggttatt cttatctttg
    20041 tgtaatgaat tattaataat tatatagaaa accgctttac tttgcaacat aatttctgtt
    20101 tatcgccaga attatcagaa aaatgacttt tacgtgtcaa aaagattaaa aaaagaagga
    20161 taaaaagctg catgcagctt tttatccttc tttgctttta tttgaaatta tttaagatgg
    20221 ctgctgatat aatctactgc gccaccgact gtttcaattt gttcagcgtc ttcatctgaa
    20281 atttccgtac cgaattcatc ttcaagctct aaaacgaatt ccataatact aattgaatca
    20341 gcttttaaat catctttgat gttcaattca tccgtcactt tatcggtatc tatctcaaaa
    20401 tgatttgaaa taattttcgc tactttgtta aatacttctt cgcgagtcaa ctttttcacc
    20461 tccaagaatc atacacttgt caattaccta caccatggct aattttactg gcatttttca
    20521 ggaatgtcca cctttttttc aatggaacgg atcgcttcag atatccaaat actattcttc
    20581 gcttttttca aattgttcga ctaattgtcc aactacatct gtctcaagca ttgtgtggat
    20641 ttggcggatc gtatagcgaa cagcatcggg gcctgttgct ccatgagttt taatgactgg
    20701 tgctttcaag ccaaacaaaa cggcaccgcc atgtttggaa taatccattt ctgctttcaa
    20761 agaacgtaag ccgtctttta aaagcatagc gcccattttt cctttgatcc cttcatttag
    20821 aatagcagat tttaatagat tcatcatatt cattgctgtt ccttcaatag atttcaaaac
    20881 agcgttgcct gtaaatccat ctgtgaccac tacatcagcc acaccattga gtagctcacg
    20941 agcttctacg tttccaataa aattcaagct tgtttcattc tgaaggagtt cataggcttt
    21001 tttcgtcact tcactacctt ttgttgcttc tgttccgttg tttaagagtg cgacacgagg
    21061 acgtccgata cctcggactt tacgtgcata aaaagaacct aaaataccgt attgcaacaa
    21121 atgttctggt ttgttttctg cgttagcgcc aagatccagc atatcgaaac cggcaccttc
    21181 tttccctact actggcaagg tagacatcaa gccagggcgt tcgatatttt tgatacgtcc
    21241 tacgatgaac aaccctgctg ctagtagcgc gcctgtattc cctgctgaaa atacagcatc
    21301 tgcttcaccg tttttcactg cttgtgcagc aaggaccatt gatgcagtct tttttcgtcg
    21361 aattgctttt actggctcat catcactatt gattttttcg tctgtatgga taatcgtgat
    21421 gtttttttca tttgttaagt atttcttgat ttctgcttct tttccgtaaa gtaagaattc
    21481 tacttctgga aaatcttgct gcgctaaagc cacgccttct acaattgctt gaggtgcgtg
    21541 atctccgccc attgcatcta cggcaatttt cattgtttcc actccttttt ccgtcatcag
    21601 tataaagtat agcacagtct gctaatcgaa ataacgatta tcctgatttt ctatttggag
    21661 gctttgcgct aaaggctgat actcaggaag catctgccaa tcctttattt gccaaagggc
    21721 tgcagcctct tctctggcta cctccaaaac atttccatcc gtcaccaagt cagcaatcgc
    21781 aaattgaggc agaccagatt gacggaagcc aaatacttca ccaggaccac gtaattccag
    21841 atctttttcg cttagtacaa aaccattggt ggtctctgtc atgattttca tccgttccac
    21901 acctagttca tttttaggat tagcaactaa gatacagtag gaagcatcac ttccgcgtcc
    21961 aacacgtcca cgaagttggt gcagctgtgc caatccaaat cgatccgcat ccatgatcag
    22021 catgacggtc gcgttcggca cgtttacccc tacttcaatt acagtagtcg aaactaatat
    22081 ctgcatctgg ttgtctttga aggcttccat gattacttct ttttcctgat tcttcatttt
    22141 accatgtaac aagccaactt catattttgg tgcaaatagt tcacgaagtt tttcatagat
    22201 ttccactgca ttttttacat ctaatgcttc tgattcttca atcagcggac agatgacata
    22261 catttgatgt cctcttgcca gttctttttt cgtccaatcc agtacactgt ccaattgagg
    22321 tgttcttacc cagcgtgttt cgatcgggat acgtccagct ggaagttcat caatgataga
    22381 tacatccatc tcaccatatg ctgtaatagc taatgttcta ggaatcggag tcgctgtcat
    22441 aaataagaca tctggtcgcc agcctttttc acgaagtact ttccgctggt tcacgccaaa
    22501 acggtgctgc tcatccgtaa taactagacc taaattctga aattcgactc catcttggat
    22561 caatgcatgt gtgccgatca cgacgttaat ttccccttgc tctagttttt caaggagctc
    22621 acgtcgttct tttgctttcg ttgaccccgt aatcaatgcg atggttactt cattgggatc
    22681 aaacagctgt tgcaagcttt ccatatgttg ttgtgccaag atttctgtag gcaccatcaa
    22741 cgcaccttgg aaacctgctg taacggtagc gtataacgca atcgcggcaa ccactgtttt
    22801 tccgctacct acatctccct gcaaaagtcg ctgcatatgc tgcggactgc gaagatcccg
    22861 acagatttca ttcgttacac gtttttgtgc atttgtcagt tcaaaaggta gcttttgagt
    22921 aaattgtttc aatttttgga catcataaag gatctccaaa ccattcgttt ctgctttttc
    22981 tgcttttttc aatccttgaa gacgcatttg aaataaaaag aactcttcaa aaacgacacg
    23041 tcgtttggct tgatggtttt cttcgtggtc ttttggaaaa tgcatacttc ttacagcaga
    23101 agctcggtca agcagtcgat atttctccac aaggttgttt ggcagatttt cttcaatcaa
    23161 tgtgccgtat tcttgaaacc cttttttgat tagatcaact aatgtcgttt gacgaattga
    23221 tttgttgacg tgatatatcg gcgaaaagtc atccattgtt tgagaaccta aaatcttcat
    23281 cccgttcaag gctttacgct ttgcatccca tttaccgtat acggcgattt cctccgataa
    23341 aatgactttt tcttttagat aaggttgatt aaaaaaagaa acgttgaaca catcatgttc
    23401 ttgcatcata cgaaattgca atcggctttt tttataacca aatcgattca tcacaggagg
    23461 cgagactacg attcctttca aagtaacctt ttcttgatcc tggatctcgt ttagatttct
    23521 ttcctgaata tcttcgtagc gaaaaggata ataagtcagt aagtcttcaa tcgtttggat
    23581 gcccaagctg gcaagactag ctgctctttt ttctccgact cctgccagca tagtgattgg
    23641 atcatctaaa gaacgcataa atttttcctc cctcctttaa caaaaaaagg acttggtcag
    23701 cgatcatacc tgatcactaa ccgaaggcct tttatgaatg aatactgtgt tagaaagata
    23761 ttctgacaca gaaattattc agcagaaaat agataaggat agacaggctg atctccttgg
    23821 tggatctcga tttccaaatc aggatgtttt tctgttagtt tctcagcaaa ttcatttgct
    23881 tgttcaactg ttccttcatc acctgccaag atcgttacga tctctgtgtc ctcattgatc
    23941 atttgttcta gtgtacttaa cgctgttgct gtgatatctg ggttagagat caggattttt
    24001 ccatcgatca tgcccaaaaa gtctccttct ttgatctcta caccgtcaat tgaggtatct
    24061 ctgatcgcgt gagtgacttg accgctcaca acgctagaca acatttctgt catcgtttct
    24121 ttgttttctt ctaaagaagc ttgatcattg aatgaaagca tagctgtcaa tccttgggaa
    24181 atcgttcttg aaggaacaac tgctactggg atatctgcta cttctgctgc ttgatcagct
    24241 gccatgaaga tgttcttatt gtttggcaag acaatcactt tatctgcgtt tacttcttga
    24301 atcgctttaa caatgtcttc cgtacttgga ttcattgttt gtccaccact gataacataa
    24361 ttagcgccta aactttcaaa cagctcttgg acgcctttac cagcagcgat cgctacgata
    24421 ccatacggga tacgtggtat ttcttttttc gcaaactcgt tcacttcttc atcgtgttcc
    24481 aaaatcgtct catgctgcaa gcgcatgttg tcgactttga ttttgactaa tgaaccgaat
    24541 ttttgtccat aattcaagac ttctcctggg ttttctgtat gcacgtggac tttgatgatc
    24601 tcgtcatcat tgaccacaag taaagaatct cctataccat ctaaatagtt acggaattct
    24661 tcgtaatcga atgtactatc gactgtaggt ccttctccga tttttaccat gatttctgta
    24721 cagtaaccaa aatggatatc ttctgtcgcc atttgacctt ggacactgcg atgatgttct
    24781 gcattgacca tatcatccat ttctgctgga gaaggttcat atgtttcatc cgcttggaat
    24841 tctccattca atgcacttaa aaagccctca taaacaaaca gcagtccttg ccctccactg
    24901 tctacgacac cgacttcttt taggactgga agcaagtctg gagttttagc taaagcacgc
    24961 tttgctcctt ttacaacagc tgtcattact tcaacgacat catcagtact ttttgctttc
    25021 ttttctccag ctttcgctgc ttctctagct actgtcaaaa tcgttccttc tactggtttc
    25081 attactgctt tatacgcagt ttcaactcca tgagtgaagg ctgcagcaag atcagaagca
    25141 tttaatgaga caacatctgg aatttgctta gaaaatccac gaaacaattg agacaaaata
    25201 acgccggaat ttccgcgcgc acccatcagc aatccttttg aaagcattgc tgctaattct
    25261 cctacttttt ctgaggttga atctactact gccttagctc cactagtcat agatagattc
    25321 atatttgttc ccgtatcgcc atctggcaca gggaatacat ttaatgaatt gacgtattct
    25381 gcatttactt gcagacgatt cgcacctgcc tggaccattt cctggaactg gcttgcactt
    25441 atttctgtta cactcaccta gataatcctc cttcagatcg actgctgagt taccagccag
    25501 tctcctgcct agtctggcag tacacgaaca ccttgtacaa agacattaac tgaattggca
    25561 gttacgccta ataatgcctc tagattgtat ttcacttttt cttggacgtt gcgggaaact
    25621 tcagagattt tcgtaccata gctgataatc gtataaacat ctacagcaat accattatct
    25681 tcttggcgga caactactcc tcttgagtaa ttttctttac gcaaaatctc agtaatatta
    25741 tctttaattt gatttttgct ggccattcct acaacaccga acacatctgt cgcggcgcca
    25801 ccaacaactg ttgcaattac atcattggta atttcaatgg ttccagctga cgtttgaatt
    25861 tttacagcca tgaatgagcc tccttaacgg gtaaaaaact cccatttttc ttctatcata
    25921 ttttatcata gcgcaacttg aaataaaagg cagttaccca aagtactcaa aaaccttgca
    25981 aaatttcagg tataaccttg tttaatttac aattattctt ctcaattgtc aattgtatct
    26041 atgaaaatgt acttgcaaat tcccattcta tatgatatgt tagtctggta tgagctgaat
    26101 tattctattg ctccaaatca aggcaaagga ggaaattacc atggcaaaag tttgttactt
    26161 tacaggtcgc aagacaaaaa gtggaaacaa ccgttcacac gctatgaact caacaaaacg
    26221 tactgttaaa cctaacttac aaaaagttcg cgttatggtc gacggaaaac ctaaaaaagt
    26281 ttgggtgtca actcgtgctt tgaaatcagg aaaagttgaa cgcgtttaat tgaatgatac
    26341 ttggaccgtt cagatagtga acggtttttt atttaataaa aatttttaag ggtgggactt
    26401 ttgattccta cccttttttt gcgtttattt ttgttatacg aacaaattat gaagttattt
    26461 attct
//
LOCUS       DACDLI010000034        25398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDLI010000034 DACDLI010000000
VERSION     DACDLI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.034"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            347..796
                     /locus_tag="IV353_001478"
     CDS             347..796
                     /locus_tag="IV353_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9009733.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            853..2271
                     /locus_tag="IV353_001479"
     CDS             853..2271
                     /locus_tag="IV353_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9009734.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            2271..3131
                     /locus_tag="IV353_001480"
     CDS             2271..3131
                     /locus_tag="IV353_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9009735.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            3305..4078
                     /locus_tag="IV353_001481"
     CDS             3305..4078
                     /locus_tag="IV353_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9009736.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            4225..4590
                     /locus_tag="IV353_001482"
     CDS             4225..4590
                     /locus_tag="IV353_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9009737.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(4738..5946)
                     /locus_tag="IV353_001483"
     CDS             complement(4738..5946)
                     /locus_tag="IV353_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9009738.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(5965..6243)
                     /locus_tag="IV353_001484"
     CDS             complement(5965..6243)
                     /locus_tag="IV353_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009739.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            6436..7092
                     /locus_tag="IV353_001485"
     CDS             6436..7092
                     /locus_tag="IV353_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9009740.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(7534..8331)
                     /locus_tag="IV353_001486"
     CDS             complement(7534..8331)
                     /locus_tag="IV353_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9009741.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(8328..9512)
                     /locus_tag="IV353_001487"
     CDS             complement(8328..9512)
                     /locus_tag="IV353_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9009742.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(9537..10784)
                     /locus_tag="IV353_001488"
     CDS             complement(9537..10784)
                     /locus_tag="IV353_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9009743.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            10999..12744
                     /gene="dnaX"
                     /locus_tag="IV353_001489"
     CDS             10999..12744
                     /gene="dnaX"
                     /locus_tag="IV353_001489"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9009744.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            12764..13078
                     /locus_tag="IV353_001490"
     CDS             12764..13078
                     /locus_tag="IV353_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9009745.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            13103..13699
                     /gene="recR"
                     /locus_tag="IV353_001491"
     CDS             13103..13699
                     /gene="recR"
                     /locus_tag="IV353_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9009746.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            13763..14407
                     /locus_tag="IV353_001492"
     CDS             13763..14407
                     /locus_tag="IV353_001492"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9009747.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            14422..14751
                     /locus_tag="IV353_001493"
     CDS             14422..14751
                     /locus_tag="IV353_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009748.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            14765..15703
                     /gene="holB"
                     /locus_tag="IV353_001494"
     CDS             14765..15703
                     /gene="holB"
                     /locus_tag="IV353_001494"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9009749.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            15739..16563
                     /locus_tag="IV353_001495"
     CDS             15739..16563
                     /locus_tag="IV353_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9009750.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            16556..16903
                     /locus_tag="IV353_001496"
     CDS             16556..16903
                     /locus_tag="IV353_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9009751.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            16972..17844
                     /gene="rsmI"
                     /locus_tag="IV353_001497"
     CDS             16972..17844
                     /gene="rsmI"
                     /locus_tag="IV353_001497"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9009752.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            17953..19074
                     /gene="dinB"
                     /locus_tag="IV353_001498"
     CDS             17953..19074
                     /gene="dinB"
                     /locus_tag="IV353_001498"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9009753.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(19128..19730)
                     /gene="nrdG"
                     /locus_tag="IV353_001499"
     CDS             complement(19128..19730)
                     /gene="nrdG"
                     /locus_tag="IV353_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9009754.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(20045..22234)
                     /gene="nrdD"
                     /locus_tag="IV353_001500"
     CDS             complement(20045..22234)
                     /gene="nrdD"
                     /locus_tag="IV353_001500"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9009755.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            22560..23327
                     /locus_tag="IV353_001501"
     CDS             22560..23327
                     /locus_tag="IV353_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009756.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            23317..25362
                     /locus_tag="IV353_001502"
     CDS             23317..25362
                     /locus_tag="IV353_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009757.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPPQRRIRRMIFEQNALTFF
                     PPMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         8077 a         4557 c         5280 g         7484 t
ORIGIN      
        1 aagtcgtagg atcataatgc acaaaacaat gaaaaactag tatatagttt taattatcca
       61 cttgataaaa acaccttata ttaacgaaat gaagaggggt tatttcgaag taaaaaacca
      121 tttacctaaa attagaagct aagctttcct ttcaaggaag gctttttgtt ttgttataga
      181 cttgcgattg tttgtgagct agatcgttca ttttaatgac ttatacacgc ggatgatggg
      241 tctttttcct ctgttcattc ataatattga acggtacagc tagcgatgct atactgacct
      301 cagttccaag aaaacgttaa cattttttgt gggggagagg ttttttatga ggtttgatca
      361 attgatcgca gaagaactga ttgttttcga tccatcgata gattcaaaag gatcattatt
      421 tgagcgaata ggggatgtgt tgaaaaaaac aaataaggta agaaatgttc aaaaagtcgt
      481 taaggcacta ttcaaacgag aggaggagat ctccacagga atagaagatg gatttgggat
      541 tccccatgca aaaagcaaat atgtgacaaa accagcactg tgtttttttc atacagccaa
      601 attaacggac tatagcggga tagatgaaat gccaattgaa tatatttttg ccatcgtagt
      661 tcctgaaaaa tcagcagata tgcatctaga gattctcagc acactctcca gacgattgat
      721 gaatgcagat tttagaaagg gaataaaaga agccactacg ccagcagata tcatcagaca
      781 gattagtcaa gaatgaaaat agtagagtct ttgtccgagt atcgatagtg aagaaaaagg
      841 aggaaaataa tcatgaaaat cgttggtgta actgcttgcc cgactggaat tgctcatact
      901 tatatgtcgg cagaaaaatt agaagtaaca gcaagaaatc taaaccatca agcaaaattc
      961 gaaacacaag gtgtaaaaac agaaaatcag ttatctgaac aagaaatcaa agaggcagat
     1021 gcaatcattc ttgcagtaga taaagaaatc gaattggacc gatttgccgg gaaaaaagta
     1081 aagcgtgtct ctacaagccg agcaatcaaa gaaccgcaag tagtaataga cgaagcattg
     1141 agagatattg gcgtattcgt tgtgtctaat gaaaaagaac cagctgcaaa tgagaaaaag
     1201 ccgacaatct ataatcattt catgaatggt gtgaattaca tgcttccatt tgtgatcgca
     1261 ggcggaatca tcatcgcact gagttttgcc tttgggatta cagcggctga tcccaactca
     1321 gcagactata atgtgttagc agctgcattt tcaaggatcg gaggagatac agcgtttgcc
     1381 atgatggttc ctgcgttagg cgcggggatt gctacatcga ttgctggaaa agcaggtttt
     1441 gccccaggaa ttgttgccgg attactggcc tctacaggtg gttcaggttt tcttggcgga
     1501 atgattggtg gcgtgctagc agggtatatc tgtgattttc tggcaaataa aattagcgta
     1561 aaaaaagaat tttctgctat ttatcagctg attgtcgtcc cctttttatc gatcttaatt
     1621 attgggcttt tgatggtatt tgtgattgaa caaccgatca catgggtgtt ggatgctttg
     1681 acaaattggc tgaatagctt gggaaataca tccgggctat tgttcggatt acttatcggg
     1741 atcatgatgg cagctgatat gggtgggccg atcaataaat cgatttcgac tttctcgatc
     1801 ggtttgatgt cagctggagt aaatgcaccg attgctgctt gtatggctgc aggaatgaca
     1861 cctcccctag gtattgcttt agccacttta ttattcaaac ataaatttac caaagaagaa
     1921 cggacttcag gtcaatcatg ctggatttta ggcgcttcgt atattacaga aggtgctata
     1981 ccgtttgcgg tatctgaccc gatccgcgtg atacctagtt tgatgacagg ttcagcagtc
     2041 gctgcaggga tttccatggc agtaggagtt acttcaatgg cacctcatgg agggatttgg
     2101 gtgatgctaa ttccaaatgt tatgaacaat ttacctatgt atgtcttggc aattgcatca
     2161 ggtacagtca ctacagcctt tatgcttgga ctattgaaaa aaacagtgaa agagaaaaaa
     2221 caagacaagt ggacgaaaaa tgaaaatcta acaaatgagg tggaagcgta atgttatata
     2281 caatgaaaga gttattgaaa gtggctaaag agaatcaatt tgcagtacca gccttcaata
     2341 tttgcagcta tgatatgatg aaagcaatca tcgaagaagc agagagatta gaagcaccag
     2401 taatcttaga aattcatcct gatgaaatcg cctatttaag tgatgagttt gttgcggcag
     2461 taagagccta tgcttataca agtaaactgc cgatcgttat ccacttggat catggcggta
     2521 cgatagaaga tgtcttacgg gcgattcgaa acggctattc ttccgtgatg atcgatgcgt
     2581 ctttgaaatc attcgacgac aatgtacaga ttacgaaaaa agtggttgcg ttagctcatg
     2641 cggtaaatgt ctcagtggag gctgagcttg gaacgattgg gaataatggt tctgctgaag
     2701 gaggtgctga aacaatcatt tatacagatc ctgatcaagc gaagattttt gtgaaagaaa
     2761 caggaatcga tacacttgct gtagcaattg gaacagcgca tggcttgtat ccgaaaaaca
     2821 gaactccaaa attaaatatc gacttactga aagaattgaa ccaacaaatc gatcttccat
     2881 ttgttttaca tggcggttca ggtaatccgg acgcagaagt cagcgaagca gtaaaatacg
     2941 gagttgccaa agtgaatttg agctcagatc ttaaaagcgt ctttttcaat gctttgagag
     3001 aaatattgag tgaaaatcca tcgatgtacg agccgaatgc aatttatcca gcagctaata
     3061 aaaaggtaca agaagtagtc aaacataaaa tggaaatttt gaacacagta ggcaaggcta
     3121 gtttatacta aaaataaaaa tgtgtggtca agagggtatg acaatagtct tgtcataccc
     3181 tcttaaccac acacgatgtt cagaagtagt tttttcgaaa aatttcttct tatttctcga
     3241 agccaatcac ttctgtcaca acctcttttt tcctgttact atataaagta aatggggtga
     3301 ttttatgttg aatgaagtgg agaatagaca gcaggagatt gtgaattatc taaaaaacaa
     3361 ccaatttgca agagtcaccg atttgatcga gttagtaaat tatagcgaag ccacgattaa
     3421 acgtgacctt gtttttctgg aaaaaagtgg attgatccgt agagttcgcg gtggcgccat
     3481 gcttattgac aatcgaaaaa ttgacgtgcc ttacctgatg aagatcacca aattagaaga
     3541 agaaacagaa aaacgtaaga ttgcagatac agcttcttct ttgattcgag atgatatggt
     3601 cttattcttg gattccagta caacctcttt acacttagtc agaaacctag caagatttga
     3661 tggtttgcag atcattacga atggcatcgt cacagctgct atgttgagtg aatatacaac
     3721 ggcaaaagta tctgtattgg gaggggcaat tgttccgaaa cgagcaacaa tcaacggttc
     3781 aaaagcctat aacgatgcat tgtcatatta tgcggattta gcgataatga gttgtcgcgg
     3841 gctagatttc agccaaggag taacagaaac tcatgaagga gaggcaatgg tcaaaaaagc
     3901 ttttagaaaa caatcagacc ggcttgccgt tttagttaca cagcaaaaaa ttgcacataa
     3961 attcatgcat caagccattg cttttcatga aatcgattat ctggtgacaa gtaaagaact
     4021 gacaaaagaa cagtatgcga aagtaaagga acaccatatc gaatgtctgt acggataaat
     4081 tattacgtga atgttctaag tggcttcctc atttttagat aggaccttat ctttgctcgc
     4141 ttctctcagc cgatggtata ataatgaaaa ggttatcttg ttagataatc ttactttttt
     4201 atccaggaga aaggagtgca gaaaatgaaa catgtgaagg cgttggtagt gaaggcaatc
     4261 atgatctggg cgattctgtg ggtcgtgctg acaggtttat acggtgttag ctttatggat
     4321 tcaacgattg ttggggtaat tattgtagtc atgatctatg tgctagggga tctgttgatc
     4381 ttacgtaaag tagggaacat cgcagcgacg atcgcagatg ctggttcagc tgcagttatc
     4441 ttgtggctct atctatattt tatgaatgat acagtcgata tttggatgaa agtattgatt
     4501 cctgctctat tgatcggtgt ggcagaatgg ttcttccaca agtggttgct aagtcaaggc
     4561 atcgtaccag atgaacggaa aatggaataa atcaacagtt aaactattct tatcgataaa
     4621 tgaaagcttt aaaaataaat agatgtttaa ttaagaataa tcataaacta tttttactca
     4681 aaaaaccgct cagattattt tcagatctga acggtttttt ttatctattc atcaagatca
     4741 aatttcattt ttcaatgatt caatcaattc gccctctgct tcatgactat gttttcggcc
     4801 attaacaaga tagagtgctg tacatatcaa tggaatgact ccggcaatca catacaatcc
     4861 tctaaatgaa gtaagagatt ttagcatccc catcaaataa ggaccaacgc ctaagcctag
     4921 gtctaaacca ataaagtagg tagataaagc aatactgatt cgatgttcgc ggacgatttt
     4981 taaacaaaca gcctgtccat ttgacataaa cgtaccataa cctaggccga ctaatgctcc
     5041 agagataagc aatgtcacac tattcgtcgt tacactcagt aaaaataatc ctgctgttaa
     5101 gaataaatag ctaggataca tcacgtattg ttctcctttg acatcgaaaa tccttccagt
     5161 caatggacgt gtaaatgtga tgaccaaagc atatacgacg aagaaaaatg acccagcagc
     5221 aactaagtgg attacttttg tataagaaga taaaaatgat aaaacacttg aataagaaag
     5281 acccatcaaa aaggcaattc ctgtaatgaa aagtgctttc ttttcaataa aactattgat
     5341 attccatgag cgaagagata ttttttgttc ttcagataat ggaatatttt ttacaggaaa
     5401 gacaaggcag gcaatcgtaa caaagaagac cagtacaatt gaaaaccaga taatgaaacg
     5461 aaaatcagtc atattcagca aaatcattcc taaaaatggc ccaacagcgg cagctaaact
     5521 agtacttaat ccataataat tgatgccttc ccctttttta gatgcaggga tatatgcagt
     5581 aacgatagca tttgtagctg tagaaactgt accgtagccg aatccattca agaatcgaac
     5641 taaatacaat gccccgatag ttggcgtata aagatatgct aatgttgaaa ggagatagaa
     5701 gatcgcgccg cctcttaagg taaacttccg accaaatagt tctaactgtt tccccatgaa
     5761 taagcgtgcc agcaatgttc cgatgatata gatcccagaa gcaaaacctg cttggctcaa
     5821 gctggcgtgt aaggtgtctt gcgcaatgac tgccatgatg accatcaaca gataataaat
     5881 caggtagaca ataaaattga tcaatgtgat tccaataaaa tatttattaa ataatttctt
     5941 ttccatgtat gttatacatt ccccttaagc tgaatatgct tttaattttt gtatttgaac
     6001 ttctacatcg tgataattgg atggatagcg atcaacaagt tggcgaataa ataaatctct
     6061 tgtagacata aacagattta aaaattcttc gcattcgttt gttggattct catccactgc
     6121 tttgatcaat tcgatcgagt tttgatacgc atcatttaaa aagactaaag aagctttcat
     6181 atcaaatctt gtttgatatg ctgtttctaa tcgattaatg attgtgttca tccagtaatt
     6241 catgtttttt ccctctattc ttaaaaaatt ttacttctgc agtataacga ataaaaaaaa
     6301 ggaaaaaaat cgcataagtg atggaaaaag gtacttaaca aatgttaata tcattagtaa
     6361 aatacctttg gactttaaaa agttttaaag tactgtatga tctttttaga tgaaagtttg
     6421 gatgggggag catcattgga tagtagagtc ttaaaggaat atttggataa aaatgatttt
     6481 ccgattatac gcaaaggata tcataaatat ttgacattcg aagggatgga agattcccat
     6541 acgtatatct tgaaagaggg aacggtaaaa gcaagtgtta tttcaaatga tgggagagaa
     6601 tttaaccttc gctatatcaa agatcttgaa atcgtctctc ttttaaaaga cgaatattca
     6661 caattcatcg attcgccgta taatattcga gttgaatcag aatatgcaga gttttatcga
     6721 atcaatcgtg tgcgcttttg gcaaaatatc aaccaagacc caaagctaca acactatgta
     6781 aaagagtatt accgctatcg tctgctttat tcgatgaaaa aaatgcagca aatgctatta
     6841 aacggaaagc tgggagctgt ttgtacgcaa ctttacgaat tatatgattt attcggtgtg
     6901 cggacagaag aagggatgct gatcgatttt tcgatcacga atgaagaaat cgccaaattt
     6961 tgtggcatct caactgctag cagtgtaaac agaattttaa aacaactgaa aaaagatggt
     7021 attatcaccg ttaaagacca tcatatcgtt attttgaata tggacttgtt agaagacaat
     7081 attatctttt gatgaacaaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa
     7141 acaaacggaa aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc
     7201 tgattgaaca ccgtttacca agaggttgtg agcctgccgt ttaactctga gtcacaagga
     7261 acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga ccgaggagtt
     7321 ggtaggagaa caccgtttac caagaggttg tgaaaaagct gttctgcatc aagtaataag
     7381 aagagccaag taaaaacagt ctcttatgtt tttaacttgg ctggacttat caccgtagat
     7441 gtaagctttt gaacgccgtt tatttgcaac aaaaggggct gtgacacgat ttttgtcaca
     7501 gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc caccgccaaa
     7561 agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag gaggtgggct
     7621 tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt tttcagttaa
     7681 tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca attgataacg
     7741 cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa cagcaatgat
     7801 gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt cgtctacacg
     7861 ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca ggtaagcttt
     7921 tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta agatcgaatc
     7981 gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt agatttcacg
     8041 ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg cgtctcttaa
     8101 gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta cgatcaagac
     8161 tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt ggatttgatc
     8221 tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag tagccaccgg
     8281 ccagccaaca cttactagca aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa
     8341 cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt cagtcccaat
     8401 tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt cattgcgtag
     8461 tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac cggcagtaat
     8521 acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt caatgttgta
     8581 tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc agattgaatc
     8641 tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc ggctaaatac
     8701 tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc gaccatcttt
     8761 tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag cttgcctgct
     8821 cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg ccatatgcgg
     8881 ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc ccaataaggg
     8941 aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt gggaacaaac
     9001 cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc gacagcaaaa
     9061 ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac gacgggatta
     9121 tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag
     9181 ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc cgctgtatct
     9241 gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc ggcaagctgt
     9301 tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct ttcttcgcca
     9361 gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc tgtatagata
     9421 ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa cggaccgccg
     9481 caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta aataaattaa
     9541 ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg gatgccgttg
     9601 gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg ctgaacgttt
     9661 tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc tgtcccgcaa
     9721 actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg tttttctgct
     9781 tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga accatttgca
     9841 ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt ttttccggct
     9901 gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct aggatcacct
     9961 agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt gtaagaaatg
    10021 ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga atcatccaat
    10081 gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga aatccgttga
    10141 ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc agctaaatac
    10201 tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc gtttgtataa
    10261 aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa gaataattct
    10321 gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt gattcccttt
    10381 atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg
    10441 aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat ggaaggagct
    10501 gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat ttttccacca
    10561 tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc tgttaaaaca
    10621 tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt gacacctttt
    10681 gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca ttgatctgcc
    10741 agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa ttcctcctct
    10801 gattcagcta gtgccaaaca attattgttt atttattata ccataggaag atgtgaaact
    10861 tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag
    10921 ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata gtgaattttt
    10981 taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt
    11041 ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc tattgtttcc
    11101 cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa gacgagtgca
    11161 gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga gccatgcaac
    11221 gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat cgaaatcgat
    11281 gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg
    11341 cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct ttcaacagga
    11401 gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat attcatctta
    11461 gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca gcgatttgat
    11521 tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt gaaagaatcg
    11581 actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga aggcgggatg
    11641 cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac aattacttta
    11701 gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag ttacttgagt
    11761 gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat tctcgcagcc
    11821 ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga cttgctgatg
    11881 tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag tacatttaca
    11941 gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat tttgagcgat
    12001 actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt cgctacagtc
    12061 aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt tgcagatgat
    12121 cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat caaggaacta
    12181 cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac aaatcataaa
    12241 aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct gcgggaagcg
    12301 acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat gagtctatct
    12361 gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc tacaggacta
    12421 gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga agaattgcag
    12481 ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga tcctgtttat
    12541 attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc acaagaagag
    12601 ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa
    12661 gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt tggctccgaa
    12721 gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc gtggaatggg
    12781 caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca
    12841 agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg tcaaagcggt
    12901 ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga
    12961 tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat
    13021 tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag gattttagtt
    13081 ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac tgatcgacag
    13141 ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct tttatacgat
    13201 cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg taaaacgcga
    13261 tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg aaatatgtag
    13321 aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag atgtaatggc
    13381 aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg tgttatcacc
    13441 gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc ggctgcatga
    13501 tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag aagcaacagc
    13561 tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg
    13621 tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga aagctgtcga
    13681 aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat ataaaagcag
    13741 cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg ggcctgatgg
    13801 tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac
    13861 agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga tccgagcagt
    13921 gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc tttatgcagc
    13981 agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt
    14041 tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg
    14101 cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc cggattttac
    14161 cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa acagacaaaa
    14221 tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac gtcatgctta
    14281 cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa gaaaaagctt
    14341 tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc aattttttga
    14401 aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga caaagacagc
    14461 aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact atcatcgaca
    14521 ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta
    14581 gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca
    14641 cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga agtagaagta
    14701 ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta ggaggagctg
    14761 agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc agcagcttca
    14821 acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg agaaaggaac
    14881 aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc aaatgaaaga
    14941 caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag aacatccaga
    15001 tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca gacgtttgca
    15061 gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc
    15121 tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag agccccctgg
    15181 agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta ctattcaatc
    15241 tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc atcgattgca
    15301 agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa acagtttagg
    15361 aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact tgatttatca
    15421 atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc aaaaaaaatt
    15481 ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct tgatgtttta
    15541 ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag tgaaacagat
    15601 aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca atgtcagttt
    15661 tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg caaaggatga
    15721 tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat
    15781 atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt attagtcgaa
    15841 tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat tgatcccaat
    15901 gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga attagaaaaa
    15961 gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag
    16021 ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg aaataaattg
    16081 attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa agatctggcc
    16141 gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga agcaaagata
    16201 ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt aggcgatttc
    16261 gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt
    16321 tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga atacgagaca
    16381 gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg taaagggaaa
    16441 gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg acgggaaaat
    16501 cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga gatgaatgga
    16561 taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa atacattaac
    16621 agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata caactttgga
    16681 gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg cacaagctcc
    16741 agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa aaatttatga
    16801 agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg acgagccttg
    16861 tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa caagaggaga
    16921 ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca gatgtataat
    16981 caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc gacgccaatt
    17041 ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc
    17101 gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat ccatacaccc
    17161 caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat tgctttgtta
    17221 aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat cagcgatcct
    17281 ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc tatcccagga
    17341 cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc atttttattt
    17401 tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa
    17461 cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac attgacgaat
    17521 ttcttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac aaaggtccat
    17581 gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga acatccagta
    17641 aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt gacggaagtt
    17701 gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa
    17761 ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac
    17821 cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa gactaatgag
    17881 gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt gtataatgaa
    17941 taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc aagaaaaatc
    18001 attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca tcctgagttt
    18061 cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg tggagtagtg
    18121 actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa
    18181 gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa atacagcgat
    18241 atttccaaac aagtacggga agtattctat cgttatacag atattgtgga accagttagt
    18301 atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc agccatcaaa
    18361 atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg ttctgcggga
    18421 gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc tagaggaatc
    18481 acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt
    18541 catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta tacaggaaaa
    18601 gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat gggctattct
    18661 ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag agagcgaaaa
    18721 tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc agtgctgact
    18781 cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca aaaacatggg
    18841 aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa aagagtgaca
    18901 ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct tatatgggag
    18961 gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac gaatctggac
    19021 ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat ataaaaacag
    19081 ctttgagagt tgaaaccgga aatccgtttt ctctcccaaa gctgttttta tatcaagtct
    19141 tgctttttga tttgttcata ggtttctgtt gcatccgtac atttttccca aatcacaact
    19201 tgattggatt ctaaagattt ttggacgtca atgatccgct ggttgctact tccgcgaaat
    19261 tgtagtttta ggtcacgttt gcttaattca aagcgtccgt caacaaggat atcgatttgc
    19321 gaaaggagtt ctaatttatc ttctgaatct tgtagcaatt catcaaaggt atagcctgtc
    19381 catgaccaga tatcttttgt cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga
    19441 caaacatctg tattcaaaaa aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg
    19501 ttgcgcaagt cagcgatgat ttgttcttct aattcaggtg tgaatggttt gccatatcgg
    19561 aaattttgtg cagctttgtt gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa
    19621 agactgttgc ggacaccttc accatcgaca aaattaaacg gtttgtagtc agcaatatag
    19681 ttttggctgt agtcttttgc taaccattct tttggttttg gatttctcat aaaaaaactt
    19741 ctttcattta aggttcaaag atatagctat tgaagataaa atctactcga ccacataaat
    19801 aagaacaaac tgacctaaat ccattgttca agaaagcttg ttcttatttt gatagagggc
    19861 gttcaaagat acaaagccat cgaaagaggg tggtgaaaaa agacttgtca tagcctctta
    19921 ttctgaataa acggtgttca aaagcttgca tctgcagtaa taagcccaac taaacgaaaa
    19981 atatgaggaa ctatttttgt ttagctattc ttattacttg atgcaggaca gctttttcac
    20041 aacctcattt catgtgtttc acacgtgagg aaatttcttt atggcggcca tgtaccatcg
    20101 gacgagcttg cggatttcct aaatagccac aagtacgttt gactacatca catgtttttg
    20161 gatcatgatt tccacattct ggacattcga accctcgttc tgtcggtttg aaatcacctt
    20221 cgaaaccaca ttgatagcaa tgatcgattg gcgtattcgt accaagatac ccgattttgt
    20281 catatgcaaa atcccataca gcttctaaag ctttcggatt ttgttggagc atagggtatt
    20341 cacagtagtg gatgaatcca cctgaacaat attttggata atccttctca aattccaatt
    20401 tttcaaatgg cgttgggttt ttacgcacat catagtggaa actgtttgta taatattctt
    20461 tatctgtgat attttcgatg atgccgaatt tttctgtatc caaacggcag aaacggtcag
    20521 ttagactttc acttggagtt gaatagacac tgaaatgata accatattca tccccccatg
    20581 catctgcatt agcttttaat tctttgacga tatcaagtgt gaaagctttt gcttctgggt
    20641 ttgtttccca ttcgccaccg aagaaagctg aagcaacttc atacaatcca atgtagccta
    20701 acgatacagt agctcgacgg tttttgaata attcattgac agaatcggta cgtgaaagac
    20761 gtttcccgaa agcaccatac atatataaaa ttggcgcatt tgctggaatg gcttctttac
    20821 aacgttctac acgatagaga agcgcatctt tcatgatttc taagcggtca gacagtaact
    20881 gccagaattt attctgatct ccttttgatt cgatcgcgat tcttggcaaa ttcaatgtaa
    20941 caacacctaa attcattcgt ccgacattta cttcttgacc attttcgtct ttccaccctt
    21001 gcaagaatga acgacagccc ataggtactt tgaaacttcc agttaattca acgattttat
    21061 cgtaattcag aatatcagga tacattcttt tcgttgcaca ttccaatgct agttgtttga
    21121 tgtcgtaatt aggatcttca gggtttaagt tcacaccttt tttcaaagtg aagatcagtt
    21181 tagggaagat agctgtccgc ttttcactcc ctaatccatt gattcgaatc tgtaaaattg
    21241 ctttttgaat ttctctttca aaccagtttt ctcctagtcc aaatcctaat gaagtgaaag
    21301 gagtttgtcc atttgaagta aataaggtat tgatttcata ttccaaactt tgcatagcat
    21361 cgaagatatc ttttttggtt tttgcttttg catattcttt ttggcgttct ggcgaatcga
    21421 tccattcttc cgcgtctttt agatgttttt tatagttcaa ttctgcaaaa ggagcaagca
    21481 attcatctgt tcggtcggca gaacagccac catattgact tgacgctaca ttggcaataa
    21541 tctgtgagat ttgtgctgtg gcggtctgga tagattttgg cgattcgact tcggcattgc
    21601 cgattttgaa cccattgttc agcatgcctt tgaaatcaat caagcagcaa ttcgtcatcg
    21661 gtgtataagg atgataatcc aaatcatggt aatggatatc gcctttttga tgcgcatttg
    21721 ctatatgtgg tggcaacatt ttcagtccga ttgattttcc aacgatcccg gctgtcagat
    21781 ctcgctgtgt attgaaaaca ttactgtctt tatttgcgtt ttcgttcacg acagtttggt
    21841 ccttgttgat tagcttttca atcgaaaagt tgatatctgt tgctttactg cgagcaaaat
    21901 ctcgtttggt acgataattg atgtactctt ctgctaattc atattcgttt ttttctagta
    21961 atatatgttc cacgatgttt tgaatctcat agattttgac attttcagca aaccggcttt
    22021 tgatttcttg gacaaccaag tctgcaatca tttgaatttt ttcataagta agtggtgata
    22081 atgagccgtg aattttcttt tctgctttga tcagtgcttc attgatcttt tgttcatcga
    22141 aatcaacatg gcggccatcg cgtttgatga ctttcatctg ggatgtctct ggctgctgag
    22201 taacgacagt tttttcttca taatttactt tcattatctt caactcctgc taaattgttc
    22261 ttgtctatca taaaacaaaa tcggggaagg agcaactaaa tatcggctat atatagtatt
    22321 caatgtgctg tataattgga tttctatact atatgtagtg tatattttat tgtgaaaaaa
    22381 ggtgaaaact tgtagctggt ggatttgttt tttgcatatt taattttgct gaataaaata
    22441 attgaataaa attttttatt cagtccaagt aaaagtgaca aaaacataag agcaaattat
    22501 tcgtagatat gctactatgc aaataaatag aaatgaaaaa tgaaagagga ggagaagaat
    22561 tggaaaagat gatcgaagtg aaacatctaa gtaaaagcta cggcagcaaa ctttctcccg
    22621 tcaaagcctt agataatatc tcattcacag tagaagaagg cgagtttgtt gggattatgg
    22681 gcccaagtgg tgcaggaaag acaacgctga tgaatatctt gtccacgatc aatttgccta
    22741 cgatgggcag tgtctcgatc caaggaaaag aaatcacaaa aatgaaaaac agtgagctca
    22801 gtgactttcg tcgaaaaaaa ctaggattca tttttcaaga gttcaatttg atcgataccc
    22861 taaatgcaaa agacaatatc ctattgcccc tagcagttga acggatgact aaagaggaaa
    22921 tggaaaagag agtaagacat gtagctcaat tgttgaatat agaagaactg ctcaaacggt
    22981 atcccgatga actttctgtc ggacagagac aacgaattgc agctgctaga gcacttgttg
    23041 ttcagccaca agtgattttt gcagatgaac caactggctc tttggactcg aagtcggcta
    23101 cagaattgtt gaactacttg aagaccatga atcaaaaaga aaaagcaacg atactgttag
    23161 tcacccatga tccatataca gcaagctact gcgatagaat tttatttatt aaagatggtg
    23221 tgattttttc tgaagttgtc agaagaggga caagaagaga gttctttgag aaagtaatag
    23281 acatgcaggc gacgattggc ggaggaggaa aaatgaatgc tgtataaact agccgttaaa
    23341 agtttttttc ggcagtcacg aggctatctt gtttattttt tcagtttgac tttatccgta
    23401 atgatctatt attccttcag cgcaatgacc tacgatcaat ctttagtcag aagagcaagt
    23461 caagatactt cgatagatgg tggattgagt tttggtggat ttatcattac tgttgttttg
    23521 ctttttttcg tattcagtgc caaccgattt ttcttgaata gaaggcaaaa ggaaataggc
    23581 atctatcagc tttttggtat gaataaactg cagatatcag gtatatatgt gttagaaatc
    23641 atgataattg gattgttcgc ctgtatttct gggatattac tcggtattat tttttccaaa
    23701 ctcttttcaa tgattttggt tcgaatgatg gatatggatc ttactagtcc attttttatc
    23761 tccattccat cagtagtaga tactttattt gcttttttca tcatcctttt agcagtatct
    23821 gtatattcaa tatggaaaat atggcgctat ccaatgattc gaagttttgg agaaaaagaa
    23881 caagcagata gttcaacaat gcgcttcagg acaaggcacc gattactcgc agtcatcggg
    23941 ttactacttt taacgagcgg atattttgga gctagtcatt ttagggaaat cacttcatgg
    24001 atgatcagtt atcattatct tgatacatta gttttctttt ttcctttttt aatttttttc
    24061 gtttgtgtgg tcggtactta tttgtttttc tgttatacat ttcggttatt tttgatcata
    24121 ttgagtaaat ggaagattta ttactatggt atcaattttt tgataatggg taacacgcaa
    24181 gttcatttgc tcaaaagttg gcgtacaaat tctctgatta cagtcattct cggaatttct
    24241 ttaatgacaa ttggcggcac ggctagtatc tctactattt tatcctacca ggagaaagtt
    24301 tcctccccga tggactatca gtttgatgtt gagacagaga aaaaaatcaa gccgattcta
    24361 gcagaggaag ggcagaagat aactaaagaa atcaccttgc attataaagt cattgggagt
    24421 atctatagtc aaaatctcga agaacctatg tttcaaactg ctaatctgat cacggaaaca
    24481 caatatcaag ccttcagaaa agttaatcca gatttgccga acatttcttt gaaatcacct
    24541 ggtcatactg ttttgcttga tcaaatgcaa actctttttc ggaatatttc attatatgga
    24601 tctagtattg gactgccaga taatcaagaa ttgaagattc aacaaatgct tccaagtgta
    24661 ttaggtgacg aaacaatggt atatctaagt cctgtactga ttgtagatga taccgttttt
    24721 gaaaaagcaa aaggtgtgga ttatcaagta gtcaatgtag atgtatcagg tggaaataat
    24781 gaaaaaatag atgaacgact gagcaatgac ctgtcgataa agtggctcaa tgctgtttat
    24841 tattcttatg atatcgtaaa tggaagtcta caaggagaaa ttagcacaaa gaaacttccg
    24901 gatgaccaaa tgaaggaagc acaatttagc caagaaagct ctcgtttgaa tttctcttcc
    24961 agctattcga aattgcgtgc agttaatcgg cgaatcggta tcaatatttt tgtcacacta
    25021 tttgtcggga tgatcgtgat tattgctact ggtagtattt tgacagtccg gcaattagca
    25081 gaagcagagg aagaaaaaga aaattatcac ctattgacta agctggggat tccaccacaa
    25141 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    25201 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    25261 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    25321 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    25381 ataataaata gaactaaa
//
LOCUS       DACDLI010000035        25274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDLI010000035 DACDLI010000000
VERSION     DACDLI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.035"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..663)
                     /locus_tag="IV353_001503"
     CDS             complement(<1..663)
                     /locus_tag="IV353_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009758.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVPVVLVNFNILKNIS
                     KFL"
     gene            complement(848..1246)
                     /locus_tag="IV353_001504"
     CDS             complement(848..1246)
                     /locus_tag="IV353_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9009759.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(1243..2016)
                     /locus_tag="IV353_001505"
     CDS             complement(1243..2016)
                     /locus_tag="IV353_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9009760.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(2013..2696)
                     /locus_tag="IV353_001506"
     CDS             complement(2013..2696)
                     /locus_tag="IV353_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9009761.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2863..3750)
                     /locus_tag="IV353_001507"
     CDS             complement(2863..3750)
                     /locus_tag="IV353_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9009762.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3747..4616)
                     /locus_tag="IV353_001508"
     CDS             complement(3747..4616)
                     /locus_tag="IV353_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9009763.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4753..6039)
                     /locus_tag="IV353_001509"
     CDS             complement(4753..6039)
                     /locus_tag="IV353_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9009764.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6312..6890
                     /locus_tag="IV353_001510"
     CDS             6312..6890
                     /locus_tag="IV353_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9009765.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6890..7819
                     /locus_tag="IV353_001511"
     CDS             6890..7819
                     /locus_tag="IV353_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9009766.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7816..9552
                     /locus_tag="IV353_001512"
     CDS             7816..9552
                     /locus_tag="IV353_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9009767.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9615..10451)
                     /gene="pheA"
                     /locus_tag="IV353_001513"
     CDS             complement(9615..10451)
                     /gene="pheA"
                     /locus_tag="IV353_001513"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9009768.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10448..10957)
                     /locus_tag="IV353_001514"
     CDS             complement(10448..10957)
                     /locus_tag="IV353_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9009769.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10950..12245)
                     /gene="aroA"
                     /locus_tag="IV353_001515"
     CDS             complement(10950..12245)
                     /gene="aroA"
                     /locus_tag="IV353_001515"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9009770.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(12258..13349)
                     /locus_tag="IV353_001516"
     CDS             complement(12258..13349)
                     /locus_tag="IV353_001516"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9009771.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13366..14532)
                     /gene="aroC"
                     /locus_tag="IV353_001517"
     CDS             complement(13366..14532)
                     /gene="aroC"
                     /locus_tag="IV353_001517"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9009772.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14534..15598)
                     /gene="aroB"
                     /locus_tag="IV353_001518"
     CDS             complement(14534..15598)
                     /gene="aroB"
                     /locus_tag="IV353_001518"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9009773.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15631..16653)
                     /gene="aroF"
                     /locus_tag="IV353_001519"
     CDS             complement(15631..16653)
                     /gene="aroF"
                     /locus_tag="IV353_001519"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9009774.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17312..18286)
                     /locus_tag="IV353_001520"
     CDS             complement(17312..18286)
                     /locus_tag="IV353_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9009775.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(18267..20150)
                     /locus_tag="IV353_001521"
     CDS             complement(18267..20150)
                     /locus_tag="IV353_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9009776.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(20168..22393)
                     /locus_tag="IV353_001522"
     CDS             complement(20168..22393)
                     /locus_tag="IV353_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9009777.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22508..23542
                     /locus_tag="IV353_001523"
     CDS             22508..23542
                     /locus_tag="IV353_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9009778.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23609..24622)
                     /locus_tag="IV353_001524"
     CDS             complement(23609..24622)
                     /locus_tag="IV353_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9009779.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24700..24888)
                     /locus_tag="IV353_001525"
     CDS             complement(24700..24888)
                     /locus_tag="IV353_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9009780.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7277 a         5473 c         4509 g         8015 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gtacaatcac
       61 tttcttttta tcttctctgt tgactactcg aaatgtgtcc gtcaatggaa gaaacagctt
      121 cttagatggt agctgttgca tacgtataaa acgatggatc atcgtaatca attgaggaaa
      181 ttgaatagac gtgtgaagca gacctggata ttttttcaac agctgtgtcg ttttgatcag
      241 attccgtttt ttactgatca cttttaaaaa gctttttaga atcttttgct gacgtctaaa
      301 aatagaaaaa gaattttcgt catctatttg ataacgcatt tgtttttgca gttctgctgc
      361 agtcagatgg atcttcccat cgtttgtatc gatcattaaa ccttctggaa acagttcaac
      421 cactgctcga tcccaagatt caagagaaag ctcgatataa tcttttactg gtaattccaa
      481 ttctacctta aaaaagtgca aaacagccga cagcccttct gcttgataga tttctcctaa
      541 tgttagcccc tctagatgga actctggtga aaaatgcagc aatgttttat tttcctgcaa
      601 ttgaacatat agaaatgtag atcgctcaat cagagtatct tttaaaacaa ctaaaaaatt
      661 catttttctc cccctttttt catcattttt aaaatagcat attttgaaaa gaaagtctga
      721 tgcttagagt agaatttcat aaaaatttac tatttcataa gtattcacga gaaaaataca
      781 aacaaaaaag cggaaataac gctcatccgc cttcttgttt gtgtttattt cctcttcaaa
      841 cgcttgtcta tgattcagac ttttctctat cgttattttc atctaaaagt tcttgcattc
      901 cgataatttc tcgatattga tgaatcttag tgataccaat gactttgtct acatcaatac
      961 taaaacaaat accattacct ggaatatcga tgttgatgcc ttgaccaatc gcatccatta
     1021 ctttattcgt caaactatcg ggtactaatg tcaacacaat atctttttct ggcgaaatgt
     1081 ccaaacctaa aaatttccct gaatcatgta caccagaacc tcttccatgt aagatcgttc
     1141 cgcctgctgc acctgcttct tttgaaaagt cgataacatc ttcgcccatt cctccgtcaa
     1201 cgatcgtcac aatcatgtga agattcaatg ctacaagatc tttcatttgc tttcccccaa
     1261 tttttgcaat ctttcttttt cgattctgtc tttttcatct tttcgtttat aaatatatcc
     1321 tagtactaaa gtggataata ttggtgtcaa tgcaatcatt gcaaccacac caaacccgtc
     1381 aatgatcgga tctcttcctt caatttgtgc agcgattgcc acgctcattg ataaaataaa
     1441 tgtcgaacac atcgggcccg ttgctactcc accgttatca aaagccattg ctaaaaagat
     1501 cttgtccact tttcgtccta aaataaatgc tagcccgtat cctgggatca agaaataata
     1561 aagagagaat ccgacgagca aacgccacat agacaatcct actgctgcgc cgatcccaat
     1621 tgaaattacc atcagcatga ttttcgatcg gattctcccc tcgctcaatt cttcaacctg
     1681 tttgatcatt acatgaatcg ccggctcagc aaaccctact aagaacccca ttaaaaagcc
     1741 aaggggaacc agtatataat tgtaaggcaa tcccgcaata gcactgccta aatgctgtcc
     1801 taccggtaca taagccatat tgactccgtg caggaaaagg atcaacccaa tagtcgttat
     1861 caagaaacct ttcataatgt caaagaaacg ctttttgcgc atacgaaaac taatgatatt
     1921 cattaaaaca aataggatta cgataggtgc cactgccaaa atgacttcca tggtaacgat
     1981 ttcgattcca tctatcactt gctgccagaa actcattgga agatcacccc caaaatcatc
     2041 acggcaagaa tcggccctaa cgaagcaata ccgaccattc caaaactgtc attttcattt
     2101 ttcttttgtt tgatcatact agtcatccct ccggcaaggg ccaagataaa tggaacagtt
     2161 actggaccag tagtcactcc ccccgaatca aaagcaatcg gcataaattc ggaactggtg
     2221 aaaaatgagg cgatcaaaac gcctatgtag cctatagcca tcaattgata ataggatagc
     2281 ttgaatacga cccgaagcag cgcaaaggct agaaatactc cagtgccgac gctgacgatc
     2341 ccaatcagca acactctgcc aatctctccg tcagatatat catatacttg ttgtcccaaa
     2401 acttgcacgg aaggttcagc aatcgtgatc acgatcccga tagcaaatcc caaactgacc
     2461 aaaacggtca aacttttctt tttgatcata tatttcccta ctagatctcc gacttccatc
     2521 atagaataat ctgctccaaa caaaaagagc gtcattccga tcatcatgat tgccgcacca
     2581 accagaaatg aagcgaggtc ttctccttct aacggtgcaa aaacaaacgt taaaatgaca
     2641 atcaggacag tcatcggcaa tatagcagca atcacttctt taaaattttt tagcatgtat
     2701 cctcctcctt tactctttaa gtttacctta aaatgaaaga gttttcgagc acaaacttaa
     2761 aacgttatca tttaattttt ctcaacataa aataacacac gtcccaaaag tatcttttta
     2821 cttggaaaaa tgactttgaa ggaacgcgct tattgtgttt ttttatttta cagagccgat
     2881 cattccttga acgaattgct tttgcaaaac aaaaaatacg atggcagttg gtaaagtagc
     2941 gatgactaat ccagccatga tcattccata gtctggtgta taggaagcac ccatagcaga
     3001 taaaacaagc ggaaccgtcc gtttatcagg agattgcaaa gctactaagg gccataaata
     3061 attgttccag ctactcatga atgtgatgat tgcagccgcg gcataggtat ttttagccgt
     3121 cggcatatac actctaaaaa aaatacttag ttcttttaga ccgtctaatc gagcggcttc
     3181 tactagatct tttggaaaag atttaacatt ttgtcgaaag aagaagatca aaaaagcagt
     3241 gctaacagat ggcaggatga caaccaagaa actgttgatt cccaaaaccg ttccgttcaa
     3301 ttgtgaaaac attcgatata aagggatcat caatgccgca aaaggaacca tcatcgatag
     3361 cagtagaata ttgaaaatta ggtcttttcg tttactttga taaatctcaa atccatatcc
     3421 tgccattgac gaaatcagca atgcaaacac agtggtcacc aaagcgatca tcgctgaatt
     3481 ccaaagggaa cgagtaaaat tcagctcatt ggaaaacaaa ttttgaaaat tgatgatcaa
     3541 gttactgcca atctttattt ttcccgatgt gatatccacg ggtgtattcg tcattcccaa
     3601 aaccatccag acaaaaggga aaatggaaat aaatgatagg atgcttaatg ctgtatactt
     3661 taatactgac ccaattgtcg aacgaagttc taatgcttgt tttttgacgt tggggcgttc
     3721 gattgtttct ttttctacca tatttttcat cgactttctc ctcctacttt cgtttggatt
     3781 atcgacaata tcacaataaa ggaaacaata acaaaggaaa ctgctgccgc atatccaaaa
     3841 ttaggcgtga atttataact caaattatag atgtattgag atagggtggt cgttgcattt
     3901 gctggtcccc cgccagtaat attttgtact tcatcaaaaa gctgcaatgt cccgatcgtc
     3961 gaagtaatgg aagtaaacaa aatgattggt tttaaatttg ggatcgtcac tttgataaac
     4021 tgctgccatt tagaagcacc gtcaatagat gctgcttcat aaatctccga atcaatattt
     4081 tgcatgcctg aaaggaaaaa aatcatattg tatccagtcc aacgccaagt gatcgaaaga
     4141 atgatcagaa ctctcgccca aattggatga gtcaaccagc caatcggtgt gtcaatcata
     4201 ccaatgccca acaacaagtt gtttacaagg ccagattgag aaaatagact tttcatgatc
     4261 agagaataac tgaccatcga agtgatacat ggaagaaaga tagcggttcg aaacaaccct
     4321 ttatatttca actttttatc atttaaaagg ttggaaataa aaagtgctag aaacaacatg
     4381 atcggtactt gtacaagtaa atacaggaat gtattaaaaa aagctttttt aaaagttgca
     4441 tctgtcaaca tccgctgata attacctaac ccgttaaatg tcatattgac agggggacca
     4501 ctttgaaaag acgtgatcaa agccaaaacc atcggaataa aaaccatgat tgatatcaaa
     4561 atgactggca agattaaaaa agcatgcccg tttttgaata aatctttctg cttcattctt
     4621 ttccctccaa ttctatcatt gattatctac atgaaaagac aagaaagaca atatctgtaa
     4681 ggcagacgtg atcttttttg tcttttcgtt tgtctgatga aaaagcgaag aaattcactt
     4741 ttttaccacc gatcaattag ccaattgtgc ttctacttgt ttttgtgtat ctgctagtac
     4801 cttgtctgtc tcttctccat tgatgattcg atgaagagct tcagctacga ctgcttcaat
     4861 tgcataggtc tcatgtccgt aattcacttc tggtatttca gatgtccatg ttgaaaaatc
     4921 gtcaaagatc ttttgaccac tgtaaaattc agaagcttct tgataagctt cttgttcatt
     4981 cgcgctaagc atcgtagata ctagtccgat ttctttcgca agagtgccca tcagttcttc
     5041 attcgaagca aacgttgctt ctaggaaatc tttcgctgaa tcttctccag ctactccttt
     5101 gatcacatac cagccagcgc cgccaaggtt agaagcttgc gctttttgca gattttctgg
     5161 aagtgcaggt attggtgcaa tcttccattt tccagattgg ttttctgctc cttggatcgt
     5221 tgaagaatac caagcgccag ttgggctaga agctactgct cctgattgta tcgcagttac
     5281 tccagcattc cagtcagagg tttgttcgac tagatcttct ttcaaaagag tggcaaacaa
     5341 ttcaagacca tactttaaag atgcattgtc ttggatcgtg actgtctccc catcttcaga
     5401 tgtataccat tctcctgcct gttgcataat catccttact cgaccaaggt cagaaggatt
     5461 gacttcagcg attttctttc ccgtcttttc tttgacatct cgcgctactt gaatatactc
     5521 gtcccacttc agattctcca tatcctcttc tgtataacct gcttctgcca tcagatccgt
     5581 acgatagaag tttgctgtta cgcccgaatc gaaaggaaca ccgtatattt tatctcctac
     5641 tttgtttacc gcaaatttat acggtgcaaa atgatcttcc tttacaatag cagacaaatt
     5701 agcgaatgca tctggataag aagtcaaata gccttgtatc ctataatctt caatcaagac
     5761 gatattgggc aatccttctg tattcccgct cgctaaagcg gtgttcagtt tctgaacgat
     5821 atcatcctga gacatcgtaa caacttctat ctctgtttct tcgttctcat aaactttttt
     5881 tgcttcattc actgctttga tattgaatgt ctcatcccac gcccagacag tgactttttc
     5941 ttctttgtct gctgtactac tagcacttcc gcatcctgtt aagaaacctg atgctgccgc
     6001 taaacaaacg actccagcaa ttaactttct gatgttcatt tttatccgct ccttttccat
     6061 ccattagttg tttacgtttg ctacattcat cataccaaaa tttgaaaacg gtttctttat
     6121 tattcagata cttcaaaaat ttctcctaaa ataataaaaa aatacaaatc aactatgaca
     6181 aaaggaataa tcttagcatc tagaccaaag atcgaaatgg tgaggtttct ttaaaataaa
     6241 aatatccttg cattttctcc tagaaataag tatttttaaa ttacaagact attcttaagg
     6301 cgggtaaaaa aatgccaata cgcgtaattc ttatcgatga tgaacagacg attttagatg
     6361 gaatgaaaca gttgatcaat tggtctgagc acgggtttca attaataggg actttttcca
     6421 gtccgctgaa agcgcttgat ttttctttgg tacatgcaat cgatatcgtg gtcacagatg
     6481 taatgatgcc tgaattaaat ggaatcgaat tatcgcggct attaaaacag caacaacctg
     6541 aaacacagat ccttatcctc tctagctatg atgaatttga tatggttaaa gattctttta
     6601 aagaaggcgt cgctgattat cttttaaagc ctcgattgag tcctgatttt tttctaaatt
     6661 ccttaactgc cgtttcacaa aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc
     6721 gttatgagca gatctgcgag aatctttcac agcagattgc tggacaaact cgtccattgc
     6781 ttgaccctct tctttttgaa cacaccttgt ttttctgtta tataccgaaa aacggccatt
     6841 cagttcaaaa caaggctcta aacaaaaaca gtcgatcacc cattcattga tggatagcga
     6901 cgattcgttt actatttatc ctgtttctac cttttcggaa gaatccggct atgtaatcaa
     6961 tactgattcc aaagatacac ttaggaagtt tgttcaaaag tttcgtcaag aagtttttcc
     7021 aggtgatttt tttgttgttt ctgattcttt tgcaatccaa gatatcaatg aaatattcca
     7081 agagatcaga aattgcacca aaggacaacg tttctatcat aagggaaaga accttgctta
     7141 tcagcatgaa cttcttccgt tattaaccaa ccacgagcag caagcaagta gttatttaac
     7201 aaaaatcgtt cataaagact acgctttagc tgtcaaagaa cttgagaatt tcttcacgac
     7261 gatattggaa cagcctattg atccagctta tttgaaacat gaggcaatca atcgtttcta
     7321 tgcattactc aacgctttgg ctgacgagca tgttgggaat gaagaacttt ctttgctcaa
     7381 gatcgccctt ccttctttat tagccgatgc tgagtatttc gaagattttt ctattcttgt
     7441 caaagaaaca cttgagaaac tcacccactt ttctgcaaat gcaaaaaaag acaatttcga
     7501 tatcttgact ttgatctacg agttcgtcca ggaaaattat gagaaagaaa tcaatttaca
     7561 acttttatcc gaaaaatatc atttttcata tagctatctc tcagccatct ttactgaaaa
     7621 atttggtatc aacttttcaa aatatctaaa aaaagtccgt atcagtaaag ccaagctctt
     7681 tttgacggaa acacggctta ctttgacgga aatctgtgag aaagtcggct ataccgaact
     7741 gggttatttc tccagagttt ttaaagaaga gacaggaatg acaccttctc aataccgtaa
     7801 aggaactttt gtaaaatgag aaaaaaaatc aaagagcatg aactctttgc caaacttctg
     7861 ttcattttgt tcatcggtct ttttctgcaa gccatcgtca tttctttgtt tatttatcat
     7921 cgatcaaggg atgcctatat ccagctgttc aatcaatcga atgatgtggt actaaaaaag
     7981 attcaatctg aatttgaaag tttgaatgat acaatcgaga atacattagc cgcttttgat
     8041 tctaatccag ctgtcaaaag ctatttttcc aatgatccag cccaacatat ggaacagctt
     8101 cagcaactaa gaacaatcca aaaaatgaat gactctcttt caaaaatcca tccaatgatc
     8161 gattacgatg tattgatctt tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc
     8221 actgccgtat ctgccgattc tttttttcaa tctgcgattg ctcagagagt gaatgaacgt
     8281 gctgctgata cacaaatgct ttttgcacac cacggattga ctcttcgcga taagaaagca
     8341 cccagcgtct tctttgtcaa aaaactgaaa aatacattga atcacgttta cggatatgct
     8401 gttttatcga tctcatccaa gcaactagcg aatctttttc aatctgtagt aaatcctgag
     8461 atatcgaaga tctccttcat gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat
     8521 acaattggga gccgatcaat gattttcaaa aaactaacag taggtgaaac aacgatttca
     8581 aatggacagc agttgacccg tcttcctctg taccgccagg attgtacatt actcagtcaa
     8641 gtcgatcttc attctttatc taaccaaatg ggcgtgattt ttccgattat tttgtataat
     8701 atcgtgacac ttgcttttgc tggctcattg gtatttatgt atttggatcg acacacaaaa
     8761 tcgatctacc gcttgattga ttcgctaaaa cggctagagg aagatggctt aaaagctact
     8821 gttcctgtac aaggaagcca tgaagtccaa ttgctaggga atacgatcaa tcaattgcta
     8881 agtaatgctc agcatcatta tgaacagacg atccaagacg aaaagaagaa acgttcgctt
     8941 gaaatccaaa ctatgcaagc acagatacag ccccatttta tttataatac gctgactagc
     9001 ttgaaatttt tgatttggca gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc
     9061 attactttac taagaagtac gattgggaaa aaagaagaag tgattccagt caaggaagaa
     9121 atcaaaagcg tacaaagtta catcgatctt ttgagtatgc gatatggcga aagaatctcg
     9181 gctaaaataa tgattcctga tgaactgcag tcattatcta tgccgaatat gatccttcaa
     9241 ccaattatcg aaaacgccta tcttcatgct tttcacaata aaacgcaagg attcatcacg
     9301 gtgtacggca aaacaaaaaa tcagcttctt ttattcgaag tgattgataa cggcgatggc
     9361 ttcgatactg aaaataccaa acaaaaaaat gatcattttt caggaatcgg catcagccat
     9421 gtagatgagc ggatacgcct aatgtatggc gatgcatatg gcttaacgat ccaatcagtg
     9481 attgggacag gaacgactgt aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa
     9541 tatcaaaaat aaagaatagc caagcgctga tttttttatt ttaagttaaa taaatcaatc
     9601 agtattcttg actttcatct acgcgtctct tctactgtca gcactggata acagcccaat
     9661 atttgacttt ctcctccgag catcttgatt tcttggagcg cattctctac taatgtcatt
     9721 ggacggtcca acaacaaatc gatcacgaaa aaatattctc ccaagctagt tttcaatggt
     9781 cgggattcaa tcttacttaa gttgatttcc cgccagccaa aagctgccag cattttatga
     9841 agcatccctg gccgattagc aggtagtgtc aagatgacac tcatttttac tggagctgac
     9901 tgctggctgg tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt caactcatta
     9961 tcttggatat tctcagctaa gatctccaat ccaacatgct gtgctgtttc taatgaagca
    10021 atcgctgctg cgtcttcttt cggatgttct gctacataca ttgctgcagc agttgttgat
    10081 tctgttgcta ctaaaggaac attaggataa tgcgtttcta aaaactgttg tgattgtgct
    10141 agtgcttgtg gatgcgaaag gatttttgta atctttgttg ctggattgcc taaaagctgc
    10201 tgcttgatag gaagaacgat ttcactttta acttcgactt ctggatgttt ggaaagaaga
    10261 tcgattgtgt gatgaacagc tccttccaat gaattctcta caggaacgac agccagatcg
    10321 acttgtttgc gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc agtcaattgt
    10381 tctgtaggaa atagctgaca agccgcctga taagtgaatg aactttctgg ccctagatag
    10441 ctgacgttca tatgcttttg atcctttcta taatggtatc gatgatcttt tctggtgttt
    10501 gattggtcgt gttgattcga tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca
    10561 ataatgcttt tatttcttct cttgtttttc caagagctaa aggtcgaata gacgtttcat
    10621 cttgttggat acgatcaact agacagtccg cttctgcctc taagtaaaga acaatcggct
    10681 gtgtttttaa aaaacgccga ttttctgcac tttggacaat ccctccacca gtcgcgataa
    10741 ttttgtcttc atttgacagc tttcttaaaa tatctgactc cacttttcga aagctttttt
    10801 caccgtaata gtgaaaatat tcggcaatcg acaattggat atgttcctca atctttgtat
    10861 ctaaatctac atactctttt tgtaaacatt gagctaaccc tttaccgatt gttgtttttc
    10921 cagcgcccat gaaaccaatc agcaaaatgc tagccatgag cttctcctcc tgacacaagt
    10981 tctgctaaat cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc tccttctagt
    11041 tcgcaaggtc catcagctat caaagcagct acttgcagca tcataccgat tcgatgatcg
    11101 cctctgctgg acacttttgc gccatgcaga ggtgtcggcc catggatgat cagaccgtct
    11161 gctgtcgctt ttatctttgc tcccattttc tgtaattctt ctgccaccgc atcaattctg
    11221 ttggtttctt ttacttttaa ttcttctgcg tctttgatga tcgtgattcc ttctgcttga
    11281 gtagccacta aagctagaat gggcaattca tcaatcagac gcggaatgat ttctccacca
    11341 atcgttgctt tcttcaaatg actcgtcttg acacttaaat cagccgattg gttctgttca
    11401 ttgtgattcg tctgggtaat cgctgcgccc atctcctcca acacatctaa gataccagtt
    11461 cttgttggat tgaccccgac attttttaac aacagttggc tttccggaac aagaagtcct
    11521 gctgctagaa agaaagcagc tgatgaaata tcacctggaa cagttatctg ttgaccagtt
    11581 aatttttgcg gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat
    11641 tgacgaatca tttcttccgt atggttacgg gaacgttctt tttcaacgat tacggatgtg
    11701 ccttctgctt gtaatgccgc aaacagcaaa gcagatttga cttgcgcact ggctacaggc
    11761 atattgtatc ggataggtga taaagagcgg gttcctgtca cagtgattgg tagatactgg
    11821 tcattttctt ctccttgcaa gtcagcgccc atctcgcgta aaggttccat cactcgcccc
    11881 attggacgtt tagacaacga agcatccccg aacagagtag tcgtaaaagg ctgtccagcc
    11941 aaaataccca tcaataaacg tatggtcgta ccggagtttc ccatgtcaat tgattcggtt
    12001 ggttggacaa atgagtggct accgcgacca tgaatcttga tcacctcttc ctcttcttcg
    12061 atctcgacac ctaattgttg gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa
    12121 aaattttgta tagtcgtcgt tccttcagca attgctccaa acatgatgct gcgatgggaa
    12181 attgatttat ctgctggtat cctaacagtc cctcttaatc catgtatttg ttgtaataat
    12241 tgcatttatc cagcctccta tttgatccag caatgataac tggtattaga cataatgatt
    12301 ctttttgctt gtaacaaatc ctgttggttt ttaaaactga tttgcagtat tcctaaaata
    12361 tcttccctgg tctcttggat cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt
    12421 ccagttactt ctgcaataac gccaggttca tctggcacat cgataaataa atcaaaaaag
    12481 gcaggaatcg cgccttgctt atggacaggc aactggtctc gactttcttt tgcccgattg
    12541 aaaaaagaaa agatttccga ttggttctca gtcatgatcc attctgctat ttgtgtcatt
    12601 tcttgttgcc aatctgccaa tcgttccaat aatatctgct tattgctcaa caaaatatct
    12661 gtccacatga caggatctga ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc
    12721 agttgtttgg ctcgcggata ctcttgattg aattgttctg cctgattgac taagccagct
    12781 gccagaatat gtggaaaatg acttagcatt cctgttatac ggtcatgttc tcttgctgta
    12841 agagttacat attttgcttt ggttccgcaa aatagttctt gcagtttttc aacatctttt
    12901 ttcgtctttt ccaaaggtgt aaagatgtaa taggcatttt caaatagttt agcatctgct
    12961 gcctgtatac ctgatttatg agaaccagcc attggatgtc caccgacaaa ccgaagattt
    13021 ttctgctcag cggatgtcac gacttgctgc ttcgtactgc tgacatccgt aatgatgacg
    13081 tgatcagaca agttgattct ttcaaggaca tcaagataag ctaaggttgt acggacggga
    13141 acggctaata agataacgtc tgcttgttct gcacctgttt caaaatcact tgcaacgtaa
    13201 tcaacgatct gctgttctac tgcttgctca cgggtttttg actgactatc ccaaccaact
    13261 actatcagtt ctggattggt ctgacgaata caaagtgcca tagaagcacc aatcagtcca
    13321 agccccacga taaaaacatg ttgtgccatc ttatttgccc cctttttagt aatgtttcaa
    13381 gtagtcacgg tactccttca ctgcagcttt caactcaaca agcgaatcac ttgggaattt
    13441 tgacaatagc tctgtcgcta ccacagtggc tacaacattt tccgcaacga cgctagctgc
    13501 aggaactgct gtactatccg aacgttctac acttgctttg taaggttctt ttgtatcgat
    13561 atcaacactt tgaagtggct tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac
    13621 aagtggttcg ccattggtca ttccgccttc gaagccgcct agattattgg tacgtcggaa
    13681 atagcctctt tcttcttccc ataggatctc atccatcact tctgagcctg gtaatgaacc
    13741 agaagtgaat ccgaccccaa attctacgcc tttaaaggca ttgatactaa ccacagcttg
    13801 tgcgattttg gcatctagct tcgtgtccca ttgtacgtag cttcctaatc cagctggaac
    13861 gcctccaacg agcacttcta caattcctcc gatcgtatct ccatttttct ttgtctgatc
    13921 gatcaaatct tttattcttt gctcaactgc tggatcaaca actcgtactt ctgacgcttc
    13981 agaaatttct ttgatttctt gaatcgagat atttccaggt atttctgctt ggattccacc
    14041 aagacaaatc acgtgtcctg ctacgatgat gtcaagttct tccagaattt tcttggcaat
    14101 tgcgccgatt gctacacgca tggtcgtctc tcttgcagag gaccgttcaa gcacattccg
    14161 aagatctcga tgatggtatt tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg
    14221 tgcaactcgc cgcagtgatt ggtctttttc aggcacttct gctacagaca tgacagttgt
    14281 ccaatttttc caatctttat tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc
    14341 gtgacggatg ccagacgtga tccgaacacg atctttttcg atcttcatcc ttcctcctct
    14401 gccgtaacca gcttgtctgc gagcgagttc gtgattgatt tcttcttctg acagaggtaa
    14461 tcctgctggt agtccttcaa taatggctgt taattctggt ccatgggatt ctcccgcagt
    14521 catataacgc atcttacagt cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa
    14581 cgatttttgc ttgcccaatc ttttctagca agatgatatt caattggctt cctcttgctt
    14641 tcttatcatg ggttatcgcg tggaataatt catctggatg acttagatcg tacgtaatcg
    14701 gcagatggaa tttttgaagt atcctttcta aagcagctgt cgatccagtt ggagacaagc
    14761 cgattttttc tgcatgagaa gtgatggcta ccattccaat agctactcct tctccatgac
    14821 tgactacccc atatcctgct gtattttcaa ttgcatggcc aattgtatga ccaaaattta
    14881 aaataagtcg atttccttgg tcaaagacat cttcttccac tacttttcgt ttgatttcca
    14941 acgtagcagt aatgattgga acagcatgtt ccagaagatc tttttcgtct ttcagctcgt
    15001 tcagctgttt ccaaagcgtt gcatctgcta tagctgctga tttgatgatt tcagcgattc
    15061 cttctcgtaa acgacgtacc ggcaaagttt ttagaacctc aggatcaatc aatacaccag
    15121 ctggctggct aaatgttccg acaaggtttt tcgctttgtt tgtattgact gctgtcttcc
    15181 cgccaatact agaatctact tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc
    15241 cacgcatgaa cgtcgaggcc acgaatgctg ctagatcacc caccattcct ccaccaagtg
    15301 caagtatccc atcgctgcgg gtaaaatcat tatctgctaa aaaatcgtat aactcagctg
    15361 cttgttctaa agatttactg gtctcacccg ccggtacgat tgaaaggact ggttcaaaat
    15421 tagcttgtct caattggaac atgatttttt ctgcatatag tggagcaacg tttgtatcag
    15481 tgatcacagc tactttttga ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta
    15541 gtgcgccttt ttcaatatga atatcgtatt tttggttatc aacatgtaca gtaagcattc
    15601 gtttcacttc tttcttttaa aaagctggaa ttaatcgagt gtctgaatct tttccattgc
    15661 ttctttcatg atcgcaactt ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg
    15721 gggaccgtct gaccaagcat ttgcaggatc aggatggatc tctacgatca tgccatctgc
    15781 tccgcttgcc acacccgcac gagccattgg tggtacaagt tcccaaattc ctgtaccatg
    15841 acttggatca acaatcacag ggaaatgagt taattttttc atcaatggga cagcacttaa
    15901 atcaaacgta ttacgtgtcg ctgtttcgta agtccgaatc ccacgttcga taaaaatgac
    15961 aggagatttg tcttcgactg caatgtattc tgcagcatta agccattcat tgatcgtacc
    16021 ggaaattcca cgtttcaatc caacaggttt acctgtttta ccgacagcag atagcagttt
    16081 aaaattttgc atattacgtg cgccaatctg taggatatcg ctgtactcag ccaccatatc
    16141 gatatgccct tcatccatca cttcagtaat cactttcatt ccaaattcat cagccgcttg
    16201 acggatgtat ttcaatcctt cttcttctaa accttggaaa gcatatggag aagtacgtgg
    16261 tttgaaggca ccacctcgca gaatttttgc gcccccggct ttggccattc gcgcacattc
    16321 tctgatttgg tctaatcctt cgatagaaca aggaccagcc atcgtcacga aactgccgtc
    16381 accaattttt actccgtcta catccacgac tgtattgcca ggatggaatt ctcgagaagt
    16441 caatttgtat gtgtttaaaa tacgcacagc attttctact ccttcatagc gtgtaaatgc
    16501 tccttcttgt aaacttcgcg tatcaccttt tagtccaatc actactcgtt ctgttccttg
    16561 atcgattgag actttgaatc cttctttttc gatatgatcg acgacttttt ttatttgttc
    16621 ttccgttgct tcttttttca taattactat cattgttttt cccgctttct tttgtttact
    16681 tatctatctt ggctgatttt ctctactttt tcaaggtggt tttacattaa ttcctactaa
    16741 atgaccgtgt cctgttgttc tcattttcgc tccttctttc ctacttatga cccagataat
    16801 caacaaaaaa agatgtccct gtaggaaatt gcctctacag tgaacatctt caacgagcct
    16861 taaaaagcgc tgcaaaaata taaaatagcc actatagatg aatttctcta cgtggctgta
    16921 agtcgtgttt aaaaatcttt agccatcata gatacaaacc tggtttgttt gtacccacga
    16981 agtcatgaat acaaacctat ctaaaatagc taaagtaata cgtattcagt tttgtaagtt
    17041 ttctagattt cataaaaaat cgctccaaac tgatgtaaga attgtaccta gtatagacat
    17101 aaaaaaatcg actgtcaatc atttttatga aacttttttt aattttctga tagttaactt
    17161 tgtttttcag aaaaataaca attggtttta ttgaagaaaa tagtcatgaa caaaagtatc
    17221 tgagaacttt tttactctct tgttcatgac atatagaata aagcagcttt gtcttgtaaa
    17281 ctccgttttt caaataataa tctaaagatt gctactgaat aataaagccg atttcatggt
    17341 cctcttcgct gctgattcgg taaggtgact caacatccgc tccccagcta ttgatacccc
    17401 cgactcctct tacttttgca aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt
    17461 ctatatgtgt tgcctgttcc agttcttctg ctttgaatgg taaagcggaa aaagcaaatg
    17521 gcgtatcctg ctgttcaaat cgcaacgaaa aatcttcttg atctttatcc gtattatcca
    17581 acgtttgatt tcttgttagt tctacccatt tcgtttccat atgcatgccg cattcttggg
    17641 gaacaaggta tggggaaacg gggagtcctg ccactttata aataccttcg acaccaccag
    17701 ccatccgatc cggatatgtt tcccctgata ggccttcata ctcaaaaaat tttgctttcg
    17761 ttggcataat gaaccttaaa cccaatgcgg gtaattccgg taatcctttt tgtccataat
    17821 aatgcatatt gacaagcatt ttaccctctc cagcgatttg gtaggaaatt gtcacttggg
    17881 tttttggtac tgtgttgatt tccagtgtaa aaatgatttc aaccgtttca gcgtattcat
    17941 tacttgaata ttgattattt actggagcaa tcggcaaatc gatcttttta ttgtccacca
    18001 gtacttctat ttctttgact tttggataca atccggctcc taaccacata ccggatttaa
    18061 atccaaatcc gctacctctg tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt
    18121 cccggtatag ccattctttt ccgtttttat tgagcgattc cagtccgcct cttgtataag
    18181 aaaaaatata atgcgtattc cccgatttga cacctaatgt acagtcgccg aagacgattg
    18241 caactttttc cgtatccata tgacttttat ttactgtatt tttcataata atatcttacc
    18301 tcctgcatgg cgggtttttc ttgtccatct gcaaaaacga tcccattccc tgagaattca
    18361 tagtcacttg gtttatcgtc aaagtcaccg ccatagcgca tcacttctct gcctgtgatt
    18421 ggatcagtca cttggatcgc ctgatcttta aaatcccata tatagcctcc ttggtacatt
    18481 tcataacgat caagcaacgt catgtaagaa tccattccac ctaacgaatt ccccatgtca
    18541 tgcatatatt cacataagat gaacggtttt tccgctttat tttcaaggta gtcaatgatc
    18601 tcttctggtg gcgcatacat gcagcttttt acatctgaaa tcgaatcttc gaatgctgga
    18661 tttggaaaat tcccttcata atggacaagt cgatcggggt ctactttttt gaagtaatcg
    18721 ttcatttgtc gaatgttttc ttccgcataa gattcatttc caagagacca aaaaagaata
    18781 cttggatggt tttttaatgt ttcgaaattc gtttttgctc gatcaagaac agccgcctgc
    18841 cactgaggta ctgcacctgg tacgttgtaa gaaggctcaa atgcgcccat tttttgccat
    18901 gagccatggg attcgaggtt cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa
    18961 taataccaag tgatctggtt ggggtaatga gatgtccgta cgccgttgat ctttgcattt
    19021 tttatgatag ccaaatcttt ttccatatct tctatagtga tggcgcgtcc ttttttcgga
    19081 tgccactcat gtcgatttac cccgttgata atcatccgtt ttccattgaa gtagatgatt
    19141 ttatctttga tttcgatttt acgaaaaccg aaggaatagt caatatactc cgttactctt
    19201 ttttcttcgt ctatcgtctc caattcaagc ttatataaat tgggctgaaa atgcccccac
    19261 agtgccacat tttcaaatcc atcgatgcga atcgtcattt gctcttttaa cggatattgc
    19321 tgttgataga tttccttccc atcaggatct agtatcgcta cattcagctg actgtttttg
    19381 gctacttgct caaaagacaa atctacttca agtttgccat tttgacattt gtcatctaag
    19441 cgaggtttga tccaaagatc ttctaaatgg ttttgtcctc gagcaactaa gaagacatct
    19501 ctgaagatcc caaaaaatcg gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt
    19561 ttgtaaacct ccacagctag aatattttct tcttccaaat aaggtgttaa atcaaattca
    19621 gacggagtga acgaatcttc ggcatacccg ataaaatgac cattcaacca aataaacatt
    19681 gcttgttcta caccagcaaa atgaatcgag atttgttttc cctttagacc ttcttctaat
    19741 cgaaaagtcg tcaaatacga accaaccgga ttgtctttag cagctgaaaa gatcccttcc
    19801 gcctctttct cagggcttgt cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga
    19861 tacatcgtat tgatgtaatg gatcttgtca taatttgcta attcaatatg actaggaaca
    19921 gtgatttcat caaaagaaca ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc
    19981 gaaggatttg gcgaataaca aaaacgccat gatccattta aagactgttt aagggaggat
    20041 gtggtttcta cttcttccct tgttgcataa caaacatgat cgctatgtgc atttgattgg
    20101 ttcactctaa aaacttgtgg atcatctaac cattttatat ttgctttcat acttttttcc
    20161 ttctctctca taactttttg aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg
    20221 ggaagtaaag tccaactgtc atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt
    20281 cctgataaag tgcctcatca tctaacccca tcagatatac ttcatggaac gctggttgag
    20341 ctgaagctag tatcctccct aaaaacacaa tagcttcatc ttgtttagga gacacaaaca
    20401 tccatgcggc aatattttct tcaaaaggac tcgccaaacg ataataatgg ccatactgga
    20461 tcaccggacg aatcgtttga taggatataa tttggtcttt tacttgcttt ttttcttcag
    20521 gagacagctt tgtcaaatcc agttcataac caaaaacagc gctcatagct gtttctcctc
    20581 ttgttttcaa cgaagtagat ctgtcggtct gatgatttgg tacagctgat acatgagctg
    20641 tgatggacga aatcggataa gcaaggcttg ttccatattg gattttcata cgctccactg
    20701 catccgtgtt atcacttgtc catgactgcg gcatataata aataaacccg gcatcaaacc
    20761 gtccacctcc accagaacag ccttcccata agatttccgg ataatcagtt gtcaaatgtt
    20821 caagtagttc ataaagcccg agcatataac gatgcgctac ttctccttgt ctctgtgggt
    20881 caagggtaat cgaatagaca tctgaaagcg aacggttcat atcccatttg atgtagtcaa
    20941 gaggaatcgt atctaaaatc tttctcattt gtttctcaat cgtctgccga acatctaacc
    21001 ttgtaaaatc cagtacatgt tgtgatctgg aagcagaagg cattctacct ggctcctgca
    21061 taagaaaatc aggatgtgtt cgatacagtt cagaatcaac cgagatcatt tcaggttcaa
    21121 accataaacc aaatttcaat cctttgctat gagcataatc tgctatttca cgtaatccat
    21181 ttgtcaactt gccttcatac tcgaaccagt ctcctaatga agaattgtcc gcatctcttt
    21241 ttccaaacca gccatcatct aatacgaaca tttcgatgcc tagctccttt gcttcatcaa
    21301 tgatcgcctt gattttttca ctattgaagt caaagtaagt ggcttcccag ttattgacca
    21361 gaatcggccg ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt agcaactcat
    21421 ggtggatctg actcattttg ttcaatccct gacttgagta agaaaggatc atttcaggtg
    21481 tttgaaaaga ctggcctgca ttcaattccc acgaaaaacg atggctgttg atcccaccga
    21541 cgactctcaa ttgattgatc tgatcttttt caagttcgaa agaatggttg ccggaataaa
    21601 caaattgaag tccgatcgct tctccagtat tttctgtcgt atgatgatga actaaagcaa
    21661 taaagttatt catatggtga ctgctcgttc ctcgacggct ttcaaatact tttgttccag
    21721 ataaaacaga ttgccgtgaa atctgtcttt cattgacatg tgctcctggc aaagcaatga
    21781 cttcatcaaa tcgcctagta tgggcaaagt ctagctggaa actagccgct ttttctagtt
    21841 tgattgtttc tccggtttca tttcttatct ttacagatcg tgtgatcact gctctatcag
    21901 taaatatggt atagaacaaa tcgaaataaa tcgctgtttc tctatcctta agtgaaatca
    21961 ccaacgtttg tgcctctgac ttgtccatca cataagtttg aggcaagcct tcgatctctg
    22021 gcttcccatc tgtaatataa tggctgtcat atctcagatc cagtaagtct gatccattct
    22081 tgcgctgaat gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa gagtattctt
    22141 gcggtagtgt atctttcgac agagaccggt caggatttaa gggaacattt cctgaaaatc
    22201 cacgatctct tcggggataa tttttattgt ctttgtactg cttgactggt ttgccaaaat
    22261 aaatatgcgc caatacattg ctttcttcta tctttatcag ataagaaaga aatgtatttc
    22321 gtaaatggaa gactcggctc ttttcatcaa ataagatgaa atttttatct gtttttctat
    22381 gcgtaatatc catcatttca ctcccaacaa aacggaaacc gtttactttt ttttataatt
    22441 cctgtatact gtttactaaa caattttgtc aataaaaatt ttttattttt ttgaggagtg
    22501 tgtaaaaatg gttggaatta gagatgtcgc acgaaaggca aatgtctctc ctgctacagt
    22561 ttcccgtgtg ttgaataacg atcagacgat ccatgtggct cagactactc ggcaacggat
    22621 agtagaggca gctgctgcgt tggattatga catcagtact cgtaaatata tcaaaaagag
    22681 aatgccttcg attggtgtga tctctacgat cggcaaagcg agcgaagaac aggatatcta
    22741 ttacaaacat ttgcgagtag ggatggaaga agaagctcga cgtctgcatt tagggatgaa
    22801 tcgaatctat aatctatctg ataatcctaa acaatgggaa gatctagatc agttaggtgc
    22861 ggtgatcgtt gttgggactg ttacgaaaga atcgattacc gccttgttaa aacaaaacat
    22921 gcaaatagtc gtcgtggata accctgacat ccagcaagag gtcgatatgg tgtatgcaga
    22981 tcttgaacgg atgactaaaa ccgtactgga attgttctta gaaaatggtc ataaaagaat
    23041 agcctatatc gggggatata aagtcgatat ggatgaattc ggtgggaagc agatttctat
    23101 aaatgaaaag aggatgcgtg cctatcagca ttttatgatc gatcatcact tagaaaaata
    23161 tatgtcttat catctaggtg gctggacaga aaaacaaggt gaggtacttg cgactaatct
    23221 attagaaaat gaatcccctc ttcctactgc cattcttgtc gggagcgatc caatggcaat
    23281 tggtgtctat cgggcattga aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc
    23341 aagctttgat aatatcgaga caatcgcaaa cctctcccct ggcttgacca gtgttcaaat
    23401 caatgcgaaa tcaattgggc aagcagctgt tcgaatggca ttggagcgaa ttgacggtat
    23461 acgggacgaa ccaatcattt tgacttatcc tgcacgattg attgttaggg aaagttttat
    23521 cccaagaaca aaaaggtatt gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact
    23581 caaaacgaaa cggctttttc acaacctctt acttatactt attcacaaaa tccaatgtct
    23641 gctggtaaca attttgcgat tcttttagcg tgtaatcaaa ttggtattca tgggtaatct
    23701 ctttttttgt atctgtataa aacaaggatt gcacgggtac ctttaattct gtcaaccgat
    23761 tttctaatgc ttgaccttgc tcctggaatg agtatgcatt tccgtcagtt atatacgttg
    23821 gcggaaattc cttagaaaca tggtcagcca cactagcttc tttcaattgc tcactcgttt
    23881 tccaatcttt tgttccaatc aacgaccacg caacggtttt gacgaaaaac ttcataaatc
    23941 gatcatccga atgctgctgt gctgttagct tcaaatcaac aggtccgcaa taagaaatgg
    24001 ttcctttcag actatctttt ggaagtagct gtttgatttt catttgttta gcatacgcat
    24061 cattcgtttg tatagctgca tattgcaatg cgatttgtga acctgcacta tctccaccaa
    24121 aaaataattg ttccatgtct agcaatttgt cgtctttttc ttcagctaat aaatctttga
    24181 tcagttcatt cacttgaaga acttgattag ggtattctga atcaggcgct aattcataat
    24241 tcatcgcaac cactgcgatg ttggcgtctg aaactaattt tgtcgcaaat tctttgacac
    24301 ccgatttatc tccgccgacg aatcctccac catgtaccca aaatagaaca ggaacaggcc
    24361 ccttgctgtt ttttggatag tagatgtcat aagtattctc tttatacttg gactcatacg
    24421 tcaaatctgt ttttttcgcc acgttttttt ctgcttgatc gaacgttttt ttatcagtga
    24481 tttgaacagt atttgaaaac atatggttga tcaagaatgc tccaggtctg ggggaaagct
    24541 ggaaggcaag aaagattgca aacgcgatcc caagaatgat tccgaagaac catccgataa
    24601 atttaagaat tttctttttc ataaggctcc ctcagtagtt agtattaagc attctgatta
    24661 taccatagtg ttatttccta tcaagctctc ctagatgatt taatgatttt ccccaaactt
    24721 cttctgcaat gcttttgaca aaagaaattt tttccattgt tcttcaaaca aaacattttc
    24781 ttcttctgtt gaagcaaatc cacattgagg acttaagcac aaacgatcca aaggcagata
    24841 tcgtgtcgct tcgtagatcc ggtcaatcac ttgctggtgc tcttccattt cgccattttt
    24901 cgttacagtc aaaatctaag acaactaatt tatcttcaga gactttctct aaaagaacaa
    24961 aactcccagc acgttcacta tcgtattcta aataataggc acggacattt tcttgagtaa
    25021 aataatgggt ctgctacgtc gtcatccccc ctgatgccgc caaaatcgaa taataatttc
    25081 cgcgtcaaac atgtatattt agtgttgaat catttgagaa tatgaaaata aaaatctttt
    25141 tgatcaatca gttaatcaaa ttgaaataac gaaaaatctg agacagaatg acgttcattc
    25201 tgacccagat ttgattttgc acaactcaac agtccttatt ggcaaatcgc caaaataatg
    25261 tagactaact aaaa
//
LOCUS       DACDLI010000036        24785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDLI010000036 DACDLI010000000
VERSION     DACDLI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.036"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(233..1081)
                     /locus_tag="IV353_001526"
     CDS             complement(233..1081)
                     /locus_tag="IV353_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9009781.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IV353_001527"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IV353_001527"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9009782.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IV353_001528"
     CDS             complement(3582..3761)
                     /locus_tag="IV353_001528"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9009783.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IV353_001529"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IV353_001529"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9009784.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IV353_001530"
     CDS             5909..8188
                     /locus_tag="IV353_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9009785.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IV353_001531"
     CDS             complement(8281..8928)
                     /locus_tag="IV353_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009786.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IV353_001532"
     CDS             complement(9107..9826)
                     /locus_tag="IV353_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9009787.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IV353_001533"
     CDS             complement(9957..11108)
                     /locus_tag="IV353_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9009788.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IV353_001534"
     CDS             complement(11325..12065)
                     /locus_tag="IV353_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9009789.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IV353_001535"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IV353_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9009790.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IV353_001536"
     CDS             complement(12449..13042)
                     /locus_tag="IV353_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9009791.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IV353_001537"
     CDS             complement(13026..13673)
                     /locus_tag="IV353_001537"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9009792.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IV353_001538"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IV353_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9009793.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IV353_001539"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IV353_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9009794.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IV353_001540"
     CDS             complement(15119..15646)
                     /locus_tag="IV353_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9009795.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IV353_001541"
     CDS             complement(15826..16611)
                     /locus_tag="IV353_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9009796.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IV353_001542"
     CDS             complement(16604..17473)
                     /locus_tag="IV353_001542"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9009797.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IV353_001543"
     CDS             complement(17474..18853)
                     /locus_tag="IV353_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9009798.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IV353_001544"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IV353_001544"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9009799.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IV353_001545"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IV353_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9009800.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IV353_001546"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IV353_001546"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9009801.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IV353_001547"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IV353_001547"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9009802.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IV353_001548"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IV353_001548"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9009803.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IV353_001549"
     CDS             complement(22811..23035)
                     /locus_tag="IV353_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9009804.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IV353_001550"
     CDS             complement(23088..24050)
                     /locus_tag="IV353_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9009805.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IV353_001551"
     CDS             complement(24060..24500)
                     /locus_tag="IV353_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9009806.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6816 a         5484 c         4285 g         8200 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt cagatatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggtagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattc
//
LOCUS       DACDLI010000037        24434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDLI010000037 DACDLI010000000
VERSION     DACDLI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.037"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(369..1487)
                     /locus_tag="IV353_001552"
     CDS             complement(369..1487)
                     /locus_tag="IV353_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009807.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1958..3265
                     /locus_tag="IV353_001553"
     CDS             1958..3265
                     /locus_tag="IV353_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9009808.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3384..4583
                     /locus_tag="IV353_001554"
     CDS             3384..4583
                     /locus_tag="IV353_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9009809.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4610..5878)
                     /locus_tag="IV353_001555"
     CDS             complement(4610..5878)
                     /locus_tag="IV353_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9009810.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6041..6687)
                     /locus_tag="IV353_001556"
                     /pseudo
     CDS             complement(6041..6687)
                     /locus_tag="IV353_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7095..7598)
                     /locus_tag="IV353_001557"
     CDS             complement(7095..7598)
                     /locus_tag="IV353_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9009811.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7721..8485)
                     /locus_tag="IV353_001558"
     CDS             complement(7721..8485)
                     /locus_tag="IV353_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9009812.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="IV353_001559"
     CDS             8592..8867
                     /locus_tag="IV353_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9009813.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9583..10533
                     /gene="yidC"
                     /locus_tag="IV353_001560"
     CDS             9583..10533
                     /gene="yidC"
                     /locus_tag="IV353_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9009814.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10655..11864
                     /locus_tag="IV353_001561"
                     /pseudo
     CDS             10655..11864
                     /locus_tag="IV353_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12174..12395)
                     /locus_tag="IV353_001562"
     CDS             complement(12174..12395)
                     /locus_tag="IV353_001562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9009815.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12556..13077)
                     /gene="tnpA"
                     /locus_tag="IV353_001563"
                     /pseudo
     CDS             complement(12556..13077)
                     /gene="tnpA"
                     /locus_tag="IV353_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13168..13534)
                     /locus_tag="IV353_001564"
                     /pseudo
     CDS             complement(13168..13534)
                     /locus_tag="IV353_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13548..13833)
                     /locus_tag="IV353_001565"
                     /pseudo
     CDS             complement(13548..13833)
                     /locus_tag="IV353_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13839..14033)
                     /locus_tag="IV353_001566"
     CDS             complement(13839..14033)
                     /locus_tag="IV353_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009816.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14033..14509)
                     /locus_tag="IV353_001567"
                     /pseudo
     CDS             complement(14033..14509)
                     /locus_tag="IV353_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14977..15330)
                     /locus_tag="IV353_001568"
     CDS             complement(14977..15330)
                     /locus_tag="IV353_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009817.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15458..15997)
                     /locus_tag="IV353_001569"
     CDS             complement(15458..15997)
                     /locus_tag="IV353_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009818.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16027..16218
                     /locus_tag="IV353_001570"
     CDS             16027..16218
                     /locus_tag="IV353_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009819.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16260..17126)
                     /locus_tag="IV353_001571"
     CDS             complement(16260..17126)
                     /locus_tag="IV353_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9009820.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17150..18802)
                     /locus_tag="IV353_001572"
     CDS             complement(17150..18802)
                     /locus_tag="IV353_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9009821.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18807..19709)
                     /locus_tag="IV353_001573"
     CDS             complement(18807..19709)
                     /locus_tag="IV353_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP9009822.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19793..21106)
                     /locus_tag="IV353_001574"
     CDS             complement(19793..21106)
                     /locus_tag="IV353_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9009823.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21174..22118)
                     /locus_tag="IV353_001575"
     CDS             complement(21174..22118)
                     /locus_tag="IV353_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP9009824.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22155..23168)
                     /locus_tag="IV353_001576"
     CDS             complement(22155..23168)
                     /locus_tag="IV353_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009825.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23248..24294)
                     /gene="gmd"
                     /locus_tag="IV353_001577"
     CDS             complement(23248..24294)
                     /gene="gmd"
                     /locus_tag="IV353_001577"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP9009826.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         6948 a         5536 c         4651 g         7299 t
ORIGIN      
        1 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
       61 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
      121 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
      181 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
      241 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggaaaa
      301 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
      361 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
      421 tccaaaataa acactttccc gccattaatg atcacatcat ctgctaatgc attcaacact
      481 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
      541 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
      601 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
      661 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
      721 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
      781 ttttctggta atgcaaataa ataaatcggc cattgttcag gattttcgaa agaatccttc
      841 aagtacgtat caacagcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
      901 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
      961 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
     1021 tctggtagag atacttcttt tacttgtttg ttgctgacct catacaattt catggagtcg
     1081 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
     1141 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
     1201 taggtctggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
     1261 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
     1321 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
     1381 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
     1441 ccttctgaag tcaattgtgt caataattct ttatcctttt ttgccataaa cgtacccctc
     1501 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
     1561 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
     1621 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
     1681 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
     1741 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
     1801 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaacttccat aacgcgcgtt
     1861 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
     1921 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
     1981 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
     2041 gcaagctgaa gatacgttca aagtaaatgc aaaagcagct tttgctgtag atgcgcaaag
     2101 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
     2161 aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactgt catggggtga
     2221 taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt tatcgaatgt
     2281 tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg caatcatcca
     2341 gtcagccaat gcttcaatgg tggccttggc agaaaaaatc tctggcagcg aagctaaatt
     2401 cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa caatcgttaa
     2461 tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca ctggagaaaa
     2521 cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt tgatcttgga
     2581 tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg aaaatacgca
     2641 atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta attacaagga
     2701 aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt tcgttggtac
     2761 gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg gccatgcgga
     2821 aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct atgacaattg
     2881 gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agtctaaaaa ccattgatgt
     2941 aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa aagtatgggt
     3001 acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca agaagcaagt
     3061 cactgacaat gagatccaag cacctatgga taaaggtaca aaaatcggta ccgctacgat
     3121 caccttggca gacgataaat taggctattt agaagacaac aatgcaccat caacagagat
     3181 cattacggat aaaacagtgg aaaaagcgaa tatattcgtg attggctggc gacgcgtcgc
     3241 agaatttttc ggcaacttgt tttaattgaa caataagaaa aacatctttc catcggaaag
     3301 atgttttttt gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg
     3361 tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgatcttt ttagttctct
     3421 atcttattgg ggtattctct atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt
     3481 tcttttacgg cagtacgtgg acatttacgg taatcttaac ttattttgtc gtgttggaaa
     3541 ttttttattc gaccttttat tttttgattc cattgttgtt ctttagtatc tttgcttttt
     3601 cttatttcac agaaaaacga aggctactga atggtctttt attcaatgtt ttactcatca
     3661 gtttcggcat ctacttgttt gtcttgctgt atgaaacgca aaatattttt cttggcggac
     3721 tgatcgcctt gatcacgatt ccattgctac ttgtcctttt atttggtatt tatggattga
     3781 ttgtttttct tttttggaac ggggtcacag ttttacgtag agaatcgcat tcattagcta
     3841 atcttttaac gttgatacta gccatttttc ttacactgtt tcttgttttt gactttttct
     3901 tgttaaaata tttgccgcaa tggataaatg ctttgttttt ctgcgttccc ctgatcttga
     3961 tctatctatt tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc
     4021 ggcctcgata caatcaagac ttcatcgttg tgttaggcgc tggattgatc aatggagaaa
     4081 cagtttctcc tcttctagcc aagcgaatca ataaggcaat tgctttttat cgggctcaaa
     4141 gccgtgctac attaaatccg cccatcttat tgatgtctgg cgggcaagga gcagacgaaa
     4201 aagtaccaga ggccattgcg atgaaacaat atgccatgga acaaggaatc cctgaaagag
     4261 acattcttgt cgaaaccaat tcgacaacca ctttggaaaa catgctctat tcaaaggaaa
     4321 tcatggatca gcaaatgaaa gggccatacc gcgcgatctt tagttccaac aattaccaca
     4381 tttttagagc cggtctttat gcgcggcaag ccaaattgaa agcaaatggt attggtgcaa
     4441 aaacagcttt ctactacttg cctaatgcgt ttttaagaga atacctcgcc atcttgatga
     4501 tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat
     4561 cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt
     4621 ttttttcttt cattctctta actattggca aaaagagtat cccttttttg accgctactt
     4681 gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa
     4741 agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag caggcaatcg
     4801 gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag
     4861 aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg
     4921 tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa
     4981 taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc
     5041 cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg
     5101 tatttcgaca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa aagacgatcg
     5161 aacggctgaa tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga
     5221 acagcaacat actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat
     5281 agctttctgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg
     5341 ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc
     5401 caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc cgttctgatt
     5461 tatccaatcg gaacaaacgt gccgtattat ctgctaacgc tccccccatt tggactgctg
     5521 ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa
     5581 gtggaataag ccgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt
     5641 tgtttccacg actagaaagt ccgccgtgat acgcatagag ataagcaaaa agcgcaccac
     5701 taactggtag tagacaaagg agccaaggat gtgatagtcg gatatctgtc acaactgtca
     5761 ggctatttaa gaaaaaagca gacaagctcc ccatgaatat accgagaatc gctgtgatca
     5821 ccagccactt cagcatatac actaaaatgc gtactggact cggtaattgt ttttccataa
     5881 aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt
     5941 aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc
     6001 cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac
     6061 gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat
     6121 catgacaaat ataattcccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag
     6181 aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg
     6241 ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag accatgaatt
     6301 ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag tcgatttcat
     6361 tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc
     6421 taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt tctgcttgcg
     6481 cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgattctg gcagttcgtg
     6541 atctaatatg tctcgaatct tttcagctgc tagtgcctga actgtttgta ccgtttttgg
     6601 atcattgaca gatgagtacg catttaataa ctgttctact tctcttatta taaaaaaata
     6661 ttggtatggt tttattgttt gttccatgat tttaaagtcc tttcttctaa ccaaagtgca
     6721 tactttgtag aaaaaagtga tcttctgcaa agtatgtgtt acctgaagat cctggactct
     6781 tttaaataag atacgttttg catcttatca tcaccccctt taatgcttat tattctatgt
     6841 acatgattca ttcgataaat aaaagtaaac cgtatcttga acagatagtc aactgcaaac
     6901 agaaaaaagg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaatg aatggtgttc
     6961 aaaagcttgc atctgcggta ataagccgaa acttcacaaa aatttgagaa gtaattttcg
     7021 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaat atggtctatt
     7081 attggtttta cctgttaaat cgaggattct ttcccaaagt aacgttgcca tctcttacgc
     7141 atacttgcag acatgggctg agccgcttct ttgactgcgc agtatttgtc cacgaatgtc
     7201 acaatatatc cttctttata cttaggcggt gcgatcgttg caccccacat gtgcttcaaa
     7261 atgatatctc gctcaagatc cgacaaatcg gtgatttttt ctgcattctt tacagcaatt
     7321 cttggatgga tatacgcatg tgtcccctcg tcaaatttcg tcgtacgcca atcatagtag
     7381 aacagatcat gcaaaagtcc agcacgcgct gttgctttag cattgccacc ccattttttt
     7441 gcgagcagat aactatagta agaaacactg atggaatgct ccaaacgagt ggaatgaaca
     7501 tgctgtgtgt aattcgctaa ttttttcact gcttctgttt ctaaaagatc ttgtacataa
     7561 gacatatatt cttcgtcttc acgccatttt tctgtcataa ttatcgattt gccacctttt
     7621 tgaatttcat tttcttctga aattgatcta agtataccac cagtgttcgg attattctag
     7681 tcgctctaga taaaatagct aaagcttaat agttccttaa tcataaagaa aaatgataaa
     7741 gaacgatccc tgctgcgact ccgacattca atgattccgc atttcctttc attgggatat
     7801 atacgttctg atcagtcgaa gacaagattt cctgacttac tccctgacct tcgtttccca
     7861 tgattatagc gaaatcctct gttttttcta ctttgtctaa tgcatgtgct tcttcattta
     7921 attctgtacc gtacaccgta aaccctttgc ctttcattac agggatcact tctgccaaat
     7981 ctttttgtaa cacaggcaaa tgataattgc ttccctgcat gcttcgcagc acctttgtac
     8041 tataaatatc tgccgttcct ttacctaata aaacaccagc cataccaaac gcatctgccg
     8101 tacggatcat cgttcccaca tttccaggat cctgtacatt atctagtaaa agccagcctc
     8161 ctgaaaaatc tggcgtatct tcagatgtta acgtcatcac tgctaagatc ccttgcggtg
     8221 taggtagatc tgacatagcg gtcatgacct catctgaaac tagaaaagta gaaatctctt
     8281 tgtttacagc cagccagtct ccccattctt tcatacctct ctgtgtcaca aaaagttctt
     8341 ttatttctgc atcagacgac gcagcttctt cgatcagatg gaacccttcc aaaagatatt
     8401 ctgccttttc ttctctgtat ttctttttat gtagtttttt taattctttg attcttggat
     8461 tttttactga ttggatctct ttcacattcc tcacctcaac tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg atttataaaa caggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg acgtgtccag ggcgtgggat
     8641 ttcgctatat gaccaagatg gttgctgatc agttaggcgt gaccggaacg gtaaaaaacg
     8701 aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga cgacatcatc caaaaattta
     8761 tcgaagaagt caagaagtct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagatccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtttttgaa
     8881 gcgctttgcc tcgagtatta aggagaaaca ttttccaaat agcttctcat attttgaaat
     8941 gtaatgactt aataccgaga ggcagctgat aaaacaccgt caataaggtt gtgaaaaagc
     9001 tggtctgcat caagcattaa ggactggttg tgaaaaatag ctctccatat ttttcaacag
     9061 gctggactta atgccgtaga tgcaagcttt tgaacatcgt cgataaggtt gcgaaaaagc
     9121 tgatctgcat caagcattaa ggactggttg tgaaaaagtc gcttagtttt gagtattaag
     9181 gaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttaa taccaagaaa
     9241 ctgacttttg aacatcgtcg ataaggttgt ttttgaggca ctttgcctca agtattaaag
     9301 acaggtagta aaaaagctga tctataactt gcaataaatt tgcttaaccc atattttcca
     9361 gacagaacaa aaaagttttt agttttttaa ataagaaaga aacacttgga atcactcttt
     9421 tctttctaag tatttctttc ttgatttttt gtcttttttc aaaatttttc gctacgaaaa
     9481 agaacgaata tttatatgaa atttctatga agttgattga attccatgcg cttttttagt
     9541 atagttaagt ttgtgtaaaa attatagaag aggaagtatt ctatgaataa atggaaaaac
     9601 tggttattgg gatccggact tgtcagtatc ctgctctttt tatctggctg tgtgcggatg
     9661 gattcaaacg gaaaccctga cacttcagga atcgtttatc gagtactcgt ccatcctatg
     9721 ggccaagcga ttacatatct agttgagaat ttcaattggt cttacggctg ggcagttatt
     9781 gcaatgactg tgatcgttcg tatcatcatc ttgccactag gtatcagtca atcgaaaaaa
     9841 acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac aagttgaagc agcacaacaa
     9901 aagctaaaag ctgcaacaac acgtgaagaa caaatggcag cacaagcaga aatgcagcaa
     9961 gtatatcgcg aaaatggatt gagtatgacc ggcggaatcg gctgtctgcc tctattgatc
    10021 caaatgccga tcttctctgc cttgtatttt acggctcgct acacagaagg tatccgtgaa
    10081 tcatcttttt acggcatcga tctaggctca ccaagtatgg tgcttgtggc catcgcaggg
    10141 ggcgcttatc tgcttcaagg atatatttca acaatcggta ttcctgaaga acagaaaaag
    10201 acgatgcgta cgatgctgat cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg
    10261 ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc
    10321 atcacaaacg ttataatgaa accgcgtttg aaagcacaag tggcagaaga gttgaaacaa
    10381 aacccgccaa aacaagtcgt tactccaaga aaagacgtga cagaatcagc aaagccagct
    10441 gacaacaaac aagcaacaaa acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc
    10501 cgcaacgcag gaaggcagca gaagcggaaa taaaaatata tgacaaaaaa ctgtagacaa
    10561 ataaaaaaat ttctttgttc tacagttttt ttgttacaat taaagaaaac gttcgcattt
    10621 cgaggaggaa tgtataatga tgtccaaata atcaatgtat agtcgaatgg aagaagacaa
    10681 aatttcgcta gatgcacttg ttcctaaaaa tcatttagtc cgtaaaattg acaaattatt
    10741 gtcatttgat tttatttatc tcattgttga atcaacttac tcgaccattg gtcgccctag
    10801 cgttgatcct gtcgtcttag taaaattagt attcattcaa tatttatttg gaatttgttc
    10861 gatgcgtcag accattaaat aagttgaaac aaacctagct tatcgttggt ttcttgggct
    10921 tagtttaatt gataaagttc ctcatttttc aacttttggt aaaaactatg cacggcgttt
    10981 cagagaaacc gatttgtttg aacagatatt tatcattatt ctcgaataag cagttgatgc
    11041 tagatttatt catgaagaaa ttttgtatat ggattcaact catattaaag cgaatgcgaa
    11101 caaacgtatt tttacaagag atatggcaca taaagaagcc attttatacc aaaaacaatt
    11161 agaagaagaa atcaatgagg caagaattgc cgaaggcaaa cgtccttttg gttcaatcat
    11221 tcgtcaggag gtggtcgaaa gaaaatttag catcgcagat ccagaaagtg gttattatgt
    11281 aaaaacagaa cgagaaaaac agtttgctta ttctgctcat accgttggtg atgaaaatgg
    11341 ttttgtatta gatgtcatga ttacgcctgg aaatctacat gatagccgaa tgctcgtgcc
    11401 acaaatcgaa cgcgttaaat cacgttatgg tgtagctgct aatgctgcgt acaaaacacc
    11461 ttggaacgtc aagtatttaa tcgaacgaaa actacgaccc attctccctt atatcagttt
    11521 cgcacaacta ctcgagatgg ctataaaaaa tatgtatcta aatcttttat ttgtgaaagt
    11581 tgtccattat tgaaaaattg tacagaaagc caaaaacacc aaaaagaaat acatcgacat
    11641 atttgggaag actacttaga tgaagcagaa catttacgcc atacggtgta caatcgtgaa
    11701 aaatataaaa aaagaaaaga aacgattgaa cgcatttttg cagacgcaaa ggaaaaacat
    11761 ggaatgcgct acaccaagta tcgaggcttg gaaaaaatca ggtgcataca atgcttgttt
    11821 ttgtcgcaat gaatctgaaa aaattagcta cttggcagtg ggagcatatg tcgttatatc
    11881 ttttattata caaaaataga caaaagtatc agtctacact cttagtaaag aggaaactga
    11941 tgcttttgtc tacagtctta gagagtggga caatagtttt ttcttcttta cgtcttttcc
    12001 tctccatgta ttttcctaat tttcttaatt taacgccatg agtgccaagc caatgtcaat
    12061 cttggctcct tgtttccttc ataagtggaa atccgtttcc gatagatcga accttctttt
    12121 tcctctaaaa gtttttttac attctgcttt gaaataacgc catgtattat tcactaaaat
    12181 ttgtcggttt cgctcacttt ttgctttcgt acattgacta cgaacaggtc agcaccgacc
    12241 actttcacat tcgtaaattt tgaagacacg tataaatcca cttttatctt ttcttcgact
    12301 aaagcgttga ccgcaaaagc gacatctgtt tcagaaatat attgttccat atctaatggt
    12361 aaaatttttt ggttgatggt ataattttta aacataaagc actcgcttcc tttctgattg
    12421 ggtttggtca ctttaatcct acagggatga gtgctttttg tgtaccttaa aatcagtaaa
    12481 tatccatccg tgaaaacggg tggcttttta acagccctag aagggctcac cactcacagc
    12541 cctatctctc tttaactatt taaatggatc tacatactct tgtttactta tactgtctct
    12601 aattcgatca tctgcttctt gttctcaaat atatatttca attgtttttt gatttaaccc
    12661 aaccgtactc acgtagtatc ctttggtcca aaagctttta ttcccatact tatatttcaa
    12721 atttgcatgt ttttcatgga ttaaggttgc acttcgtcct tttaagtagc ccataaaact
    12781 agaaatacta atctttggcg gtattcttac taacatatga atgtgatcag gcattgcatg
    12841 agcttcaatg atttcaacat ctttcctctc acacaatttt cttaatatct ggccaatact
    12901 tactcttaac tttccataaa tcacttttcg tcgatacttc ggggtaaaga ctatgtgata
    12961 cttacaatcc catctcgtat gtgctaaact tttatcgcta aagctatttc gtccacgcgt
    13021 agaaacgcgc gtttttttgt ttcgtacgct acgccaacga aactgactaa agtcaataaa
    13081 aaaagtgggg caataatcag atttttttct gactattgtc ccactctctt tttattactc
    13141 ttcatcttct actattccac taattaatta gttgacagat acaatgcttt cacatcctgc
    13201 acgaacaaac tcgggctctg aaccaatcac ttgcgagttg ataaattcaa tgaccatagg
    13261 aagattaatc cgacataaag tttaatctgt tgtccttcca ttatcagttt agaaatgata
    13321 ttagtaggtg taccgcctaa aagatcgcat agtaccacaa aatcatttaa cgaatctatg
    13381 acagtaagag cttttgtctg tatgtttcag tgccatcttc tggtaataaa ggtaacgtgt
    13441 agatattctc ttgaggtccc ataatcattt ttgtactttt tttcaattcc tcacaaaatt
    13501 ttccgtgact aatcaatatc aattttctac tcatttttgg ctctccctta tttcgaaatc
    13561 acacacaatg cagataaagc cacacacaag taatacgata aaaatcgctc gagttgggtc
    13621 atatttttct ttcctaacaa ccaataaaca cctcgacgat cccagcgagt aagattttag
    13681 ataatatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc gttggtacca
    13741 taaaaattta atatcaatca ttgttgcaac taatgctcct accatgaaca ctcttaataa
    13801 agtcgctacg tccgttaata cagttaagcg agattacact agatgatctg ttcctgccaa
    13861 ggcaagagcc agaagcggtt gcctgcccat ttgcctttgg tgtgtcggag ctgtccgata
    13921 aagttcaccg cccgatccgc tctggctttg tcgtagtgac attccgaaag catgaatcgg
    13981 cttggtgtgt attcctttaa tttgggatag ccctttggtc tggtttctgc catcagctac
    14041 cacctcccaa cagagcctcc atcgcatctt ctgctccgac accaccgcca tcaccggcaa
    14101 tgatacggct tctggacgaa ggcgtcagac cgaactgctc ggaaaatcgg ttcataattt
    14161 tcagataggt ctgggcaata aacacctgtg gtacctgctg ccaatagccg gatagggttt
    14221 tcacgatgat accgtgctgg gtaatgaatt cctcggcttc cttccagcgg gcgtaggctt
    14281 ggcactaacc ggcaaaggca gccatatcca catctgtcaa gattccgatg gcctccatct
    14341 gctttgccag acgcttccat tccttctttg cctctggttc cagccacttc ggacaggtcg
    14401 gtgccttctt ttccggcgtt gactcttttg cattgagtgg gcgattgccc ggatttccct
    14461 ccagcttctt catggctgtc ggggttgatt ttcttcctct ggttgccaca gtcgtcacct
    14521 cccttcaatg tatttctgcc agtagacaga gaacagagcc aagtcgaaac ctgactctct
    14581 tctctatctg ctaacttgta tttcagtcca ctcttctgat gaatcgcacc cgcaggattt
    14641 tcaagatgcc gcaggcaaat tccaagaagt ttctgaccgc atcataacca tcgtagccgt
    14701 gtagccacat ccttccacct cgccgccgtc ctcgtcgctg atataaatga ttctgtattc
    14761 gctcatctgt tgttttctcc agtcttttgt ttttttgtag tacatatatc actctaaaag
    14821 tgattattat caagtcgttt gaaagccata atgtacacaa tcattcgact ggtttttggt
    14881 tcataattga ccgccaaagt gagcaaaaga aagggacctc tgtctgtttc agaagtccct
    14941 ttcggtattt ggttcttagt ggttcaggct gtattctcaa acctcatact catcttcgag
    15001 gtctgcctct gcaatgatct ggccgatgct gtcgtaacgc tggacctgca tctggatggc
    15061 atccttgtct gctccctcgt ccatcatctt cagcagcttg tctgattcct ttttgtaggc
    15121 ccagaaaagg atctcctgaa ggctggtctc ctgctttttc ttgcgaaggg cgaccttctt
    15181 ctcttccacc gcctcgttca acttggtgaa ctctgcccaa gcaaggatac cgtctcgctt
    15241 gattgcgatc tccacatcct tcagctccgg gatggtcttt gcacccttga tctccttcaa
    15301 atatttcttt gccttttcgc tcattgccat tagcgtttcc tccttgtttt tgggattttc
    15361 ccctttggtt gtgtgtatat tgccatagtc tctcgactat atcaagtcaa tccacaggca
    15421 tatactacac aatcatttgg agctgtattt gtatacatta tgccgattta tgagagaaga
    15481 aaaggctcca gccgttcgac tggaaccttg ggggggtcgg tttcttaggc ggcgtggcgg
    15541 tgtagacctt gaggtagctg atgttcttcc agacgtccgt gcccttcagt ctgaccatgc
    15601 cgtccagctc tcggtcgagg gtgaacttgc ggaaaagctt gatgcggttg aggctgtttg
    15661 ctagctcctt gccgttcttg gcatccagaa ctgcgtcctc gatctccgca atactaattt
    15721 cctgtctaaa ccaaaggttg ctcaaggctt cgggaatgcc gtttgcggtc tgcagggggt
    15781 tgaaagtttc caatacagca ggggtcgaaa ccacctgtcc gagctggaat tttgcgttcc
    15841 ctaaaatcat ctgcataatt tcttccatgt gaatcatcca tttctgccgt tgtggtattc
    15901 cctttggcag tacagtacta tatatcactc tgaatcgtac atttatcaag aagcggattt
    15961 ttgtggaatc accaattcga tataatagta attgcatgaa aaatctaagc actggattgg
    16021 taggtgatga ttatacgtga cgatcacatt tacacctgtg attcctgcca ctacagcttc
    16081 cctgccgacg aacagccaga gcgttgtcct gactgcgaaa agacagcaac tcggctggat
    16141 accgaaatcg aaactgaaga ttactatcgt gttcgtgctg aaatcaaagc tgagattaaa
    16201 gctttgaatg cgggttaacg acaggtactg accggcggct caacaccgcc ggctttttat
    16261 taccacatct tgcggaattc gctgggacga acaccttcca ccttgaaaaa agttctggaa
    16321 aaatagtttg catctgttat gccaattttt gttgcaatct cttccatact tagatttgta
    16381 aagcgtagca agtgcttggc ctgtgtgatt cgtttctgat tcagataacc gacaacagtt
    16441 gtaccgtaag tttcacgaaa aatcttagct aagtactgtt tattgataaa aaactctgca
    16501 ctcaactgat ccagcgtaat cttctccgtg taatggtcat ccagatattg ttttatgttc
    16561 tgcaattcca ttttttttgt agagaaattc ggcactttgt caggattcca agaatcttcc
    16621 atcaaatgca tcagcagttc actgagtaga acattgattt tcatatcccg gacatactcc
    16681 tgtccctctg ccaccatatg aaggctcctc agcaagtcag catacttatc tgtttttgat
    16741 gttttaaaga ccggttgacc acctcgttca aggtattttt tgtagacttc cgtcatacca
    16801 atgccattaa aatggcacca ccgcaaagtc cacaaatcat cttctgatga ctcatggaca
    16861 tacggcttca aacagtcaat aaacaccata tctcctactt gaagcgtata tttctgcccc
    16921 ttatactcca atgtgcctga accaacttct accaggaaac aaaggtaaga tgacaatcct
    16981 gtacgcctag atgtatgtgc cttcagagca gtcagctgtc caatttcctg caaatacaaa
    17041 agagaggatc ttgcaaagaa cgatgccgtg tacagcacac gatttgactt caccgatttc
    17101 aaagcgttag aaaacagttc ctccattaca agatttctcc tttcttttct tactcgaaca
    17161 tttccgccag ttcagcagcc ataatagctt ccatagcttc ggcggcttcc ttgttctctg
    17221 cgctgacgct gatataggtc ttcagcttcg gctcagtacc agaaggacga accacaacag
    17281 agcagttatc ttccagcata aacttcaaaa catcagactt cggcagacca ttcaggccct
    17341 tactgtagtc taaagtttca ataaccttct taccaccaat tatatcgaga cccttgtgga
    17401 attcaaccat aatttcctgc atcttggtaa aacctgccgc accgtcaaac tcgaaggaat
    17461 gcagggtgtt caaacaatag ccatactttg tataaatctc gttcagctta tccagcaggc
    17521 tgataccctg agtcttgtag taagagaaca tttcacagat tagataagca gcgttaacac
    17581 cgtccttgtc acggacatag ctaccggaca gatagccgta agattcctca aagccgaaga
    17641 tatagctgtc aaccttacca gccttttcca acataccaat ctgctcaccg ataaacttaa
    17701 agccggtcag cacatcgatg gtacgaacac catagttgga tgcgatacgt tcacccaaat
    17761 ccatggtgac gatggtcttg accatcacag ggtcagcagg catttttcca tgcttaattc
    17821 tctgagaaca gatgtagtcc agaagtagca gaccagtctc attaccagac aacagaacat
    17881 agtcaccatg tgcgctctta actgcgatac caacacggtc gcagtcaggg tcagtggcca
    17941 acagcagatc cgcattgtac ttcttggcat actccatacc cagagacata gcttccttaa
    18001 tttcagggtt cggataagga caagttggga aattaccgtc cggcagacgc tgttcttcca
    18061 caacggtcac attgctgtat cccatctctg ccagggtgcg ggtaacaggc accagaccgg
    18121 taccattcag aggagaatac acgatagcaa cattcttgtc cacttcttcg ccaaacagga
    18181 cggactgccc cttcacttct tcgatgaaag cagtcagaac ttccgactga atggatgcaa
    18241 tcttagaagc atcgccatcc atctttacat cagcaaagat gtccagcttt tcgatttctg
    18301 ccagaattgc agtagcagcc tcggtagtaa tctgacagcc gtctgcaccg tataccttgt
    18361 agccgttata tttagaaggg ttatgagatg ccgtaatcat aacaccggca tctgccttca
    18421 gataacgagt cgcaaaagat actgtaggaa caggaagcag ttctggccac aagtgcacct
    18481 gaataccatt tgcagcaaag atgtctgcgg cagtctttgc aaacacatca gatttgatac
    18541 gggaatcata gccaaccacc acggaagcgg cttcatagtt cttattcaga tagtctgcca
    18601 gaccttggct tgccttacct acggtgtgta cgttcatgcg attagtgcct gcaccgatgg
    18661 taccacgcag accaccagta ccgaaagaca aatttttata gaaagagtcc tcaatgtcct
    18721 tctcagaata ggaacgcagt tcttctataa tatctgcatt ggcatgattc agccaacgct
    18781 catattctac tttataattc atattattat cctctcacat ccagaaactg aatcgacccg
    18841 tgaatgctga tttcagtaga atcggccggc acaaaaaggc agtccccctt atagatgtcc
    18901 aacgattcat tatcaaatcg aacggtacca cagccattga cacacagcaa cacacggaag
    18961 ctgattgaat ccgcacgata gtgtaccgtc tgtcttctct cagtgttgac aatcatacga
    19021 gagacctcga aatgcttgca tctgcaaagc aattccgcag ctacaccagc acgatatttt
    19081 agaacacgaa gcggctgttt cggttctgac aaccctttca agttcgctac ttccagcgct
    19141 ttgtctacat gaagttctct ctttttacca tttttgtcaa cacggtcata atcgtagaga
    19201 cgataagtca gattggaatt ttcctgaatt tctgcaatta gtgcacccgc accaatcgca
    19261 tgaatcgttc ccgcctcaat aaagaacaca tcattctttt tgaccggaac cttctgaaga
    19321 taacgctcaa cagttccctg agcaatcgct tcacggatca cagcttcttc tgttattttc
    19381 tgcagcccat agattaagct ggcatccagt acataccaca tctctgtctt tccaagctgc
    19441 ccgttttctt tttcaattgc atattcatct gtgggatgta cctgaacaga caaatccttt
    19501 ttcgcatcaa tgaatttgat caggatcggc aattctcctt tggtctgcgg atgcgttccc
    19561 agaaactctg ggtgttcttg caacacctcc gtcaaggtca tcccctgata ctctgaccag
    19621 acacaacaaa gctcggtcca tccgggtgcg tagagcattc caagtttccg caagaggaga
    19681 catctcaatt ccttttgcaa attcatcatt caggcggttc ccccccacag ataatccttt
    19741 gcggaaggct tcaataaaaa cggtttctgt ataacgagcc ctcctccgtt ttttaatact
    19801 ctaactcaaa tttctgttta tctttaacat tgatatcctt gccaagctgt acttcaatca
    19861 gcttcagctc agtatctgcc ataatggtat gccgacagcc agcactcatg gtgatcacat
    19921 cgccagcctt aacaacctgc tcatatccat ccacgatggt cttaccagta ccggccagaa
    19981 ccacccacac ctcgtcacgg ttcttgtggc tgtgataatt catggagtga ctagcattca
    20041 gagtaacctt aatggtcatg ctctctgctt ctacatccat aaccttataa ctaccccaag
    20101 acttctctgc gaacatgatc tgctgctcga acttgtcaac gaaaggctta atatagctgg
    20161 actgttcctt atcggaaacc agaatacctt cccgagatgc acagatgacc acatcatgca
    20221 gacccatagc caacacagga acatccagtt cgttcaggat gtgtacgcca tcgcaattct
    20281 cattcataac acctttaccg ataatggatt cctccatacc ttcggtcaga gtattccaag
    20341 tccccagatc cttccacata ccagcaaaac gctgtacctg aatcttgtcc tcatgctcaa
    20401 caacggcata gtcgaaggaa atcttagtca gagtatcgta tttgttgaac agatcctgat
    20461 aatctacaaa gtcaatcagc tgatgtgcac gctccatgac atacttcagc ttgtagacaa
    20521 acacaccacc gttccacagt gcaccctgtg cgatatattc ctttgccaca gcctcagaag
    20581 gtttttcctt gaaggtattt acgaaagcag tctgctctgc gctctcagga atgatgtagc
    20641 cgtacttctc ggaaggataa gtcggttcaa tacccatgag caccagatta gcttcgcctt
    20701 tgtcagcctg atcggacaga gatttcaggg cctcaaaata gtcatcttct acgtaagggt
    20761 caaccgggca aacaactacg gactcttctg gatcaatgcc catcacatct accagataag
    20821 cggtagccaa tgcgattgcc gggaaggtat cgcggcgaca aggctctaca gatacaccca
    20881 catcctcacc aagttggtta tggatagcag aaacctgtgt cttagaggtt gcaatcgtaa
    20941 cagttgcgtc gttatctacc ttcttaatct ggcggtagac acgctgaacc atggattcat
    21001 atccatcttc agttttgaaa atcttaatga actgcttgga acggatgtca ttagacagcg
    21061 gccaaaggcg cttaccagaa ccgccagaca gtaaaacaat attcataatg atctctcctt
    21121 tttataagaa agaggctgct gcaatcctca aaaatcacag caacctcggt aaattagcgt
    21181 tcagcacgca tcgggttatt caggaagtcc tcgtaggaca gacggatgcc atcttccaac
    21241 tcagtcttat atgtccatcc cagcttggtt gccttggaaa catccagcag cttacgaggt
    21301 gtaccgttcg gcttgctggt atcccacaga atctcgcctt cgtaaccaat aacctttgct
    21361 accatctcgg tcagttcctt gatagacagt tccttgccag tacctgcatt aacagtctcg
    21421 tcaccgctat agttattcat caggaacaca cacaggtttg ccagatcgtc aacatatagg
    21481 aattcgcgca gaggagaacc atcaccccag caggtaacag taggcagacc tgcttcctta
    21541 gcctcatgga agcgacgaat caaagcgggg agcacatgac tgtgagtagg atgatagttg
    21601 tcgttaggtc catacaggtt agtaggcata acagagatat agtcagtacc gtactgcttg
    21661 tttagaaact cacagtactt caggccagaa atctttgcca gtgcataagc ctcgttggtc
    21721 ttttccagct cacttgtcag cagacagctt tccggcatcg gctgcggtgc catacgcgga
    21781 tagatacagg aagaaccgag gaactgtaac ttcttacagc cattctccca tgcggagttg
    21841 atgacgttca tttccagaat catgttttca tacatgaagt ctgccagagc gttctggttt
    21901 gcgataatac cgccaacctt tgcagcagcg agaaatacat aatccggctt ttcctgtgcg
    21961 aaaaatgctt caacagcgtc ctgacggcac aggtcaagct ccttatgggt gcgggtgata
    22021 atattgttgt agtcctgacg gttcagctcg cgcacgattg cggaaccagc catgccacga
    22081 tgaccggcta cgtagatttt tgcgtctttt tccatcataa taatgcttgc tcctttctaa
    22141 atttagagca tctctcattg catgagagaa atcaatttgt ctgcgatttc gctcgcagca
    22201 tcataaaatg tatcaaaatt tgaggtgtct agctgtttga ggtcctcaat cacccgctct
    22261 acgcggtgga ggttttccgg tgtgacggaa attgccagac ccagtttttg caatttacaa
    22321 atagttgttc tatcttccaa aactgtatcg cgggcgaaca gtgccatcgg acgctgtgcg
    22381 agaatacact ccgcaacagt tccccatcca gcctttgtaa taacgtaatc tgccgccgct
    22441 atatagtcct gtgtattggg ggtgctgtta gaaataacgg ttacgttttc tcccacaagc
    22501 ggaacaccta gcgttgtaat gaaatcatac ggcgcgtgac tgacatcaat cggctgtttg
    22561 aaccgcgcgg acataccgag tgcgacaaat acaggcggtt tttcatgctg tgatttaatg
    22621 cgttgcactt cgtcgttatg aatcggacgc gccacaacgg aagtcggtgt tttttcgctg
    22681 ttttggagaa attccaacat ttcttcgtta tttagcgcat aaagcatggt gtgctgaatt
    22741 ttaccgtaac gcttggcata ttctttccaa atatgttcgg gtaaaaattc gcgatacagc
    22801 tctgtccaag tgaagtttcc tacatacagg aaaggtactc ctgccatatc gcaggcatcc
    22861 attgcccaga ttggcatatc gcaaagcact gcgctgacag cttctcgctt gagccattcg
    22921 gcttctgtct ttgccaaaga aggaaccttt gccaaaaaat attcacagat ctcggtcagc
    22981 ttacccgtgt ccaccagtag ggtgccctca cggttaatca gaccgatatc tgtgtttatt
    23041 ggacgatagc gcacgcggtc tatcagctct ttgggcagtt cctgctccaa attgcgcttt
    23101 gcaaaatcca gatgatttgc gccgcaaacc agtgtaatct tcgcttcgca tcggcagagt
    23161 gttgccataa taatgggcag attccgcgcg atatgtccga agccgtgact ggaaagatag
    23221 aaacagatat gtttattcaa tgtcagctta cttgactgcg ttcttcattg cagcttcgcg
    23281 ctttgccaat tcacggtcat gctttgccat gatgttaacc agttcctcgt aagaggtctt
    23341 ctgcggattc caaccgagaa cggtctttgc cttagtcggg tcgccccaca ggttgtcaac
    23401 gtcagtcgga cggaaccact gtggatttac acaaacgagc atcttgccgg tttcaacatc
    23461 gtagcccttt tcatcaatgc cagtgccttc ccaacgaatg gtcatgccat ttgctgcgaa
    23521 tgccttctcg gtgaagtcac gaacagtgtg ctgctcgccg gtagcgatca cgaagtcctc
    23581 gggagtctca tgctggagaa tcatccacat gcactcaaca tagtcctttg catagcccca
    23641 gtcacgcagg gagtccatgt tgcccagttc cagatggtcc tgcaggcctt ctgcaatgcg
    23701 gccggcagcg agggtgatct tacgggtgac gaaattctca ccgcgacgtt cggattcatg
    23761 gttgaacaga ataccgttga cagcgaacat gccataagct tcacgatatt ccttagtgat
    23821 ccagaagcca tactgctttg caacagcata agggctgtaa ggatgaaacg gagtggtttc
    23881 acgctgagga acttcttcaa ccttgccata cagctcagaa gtagatgcct gataaatctt
    23941 agtcttcttc gtcaggccca gaatacggca agcttccaga atacgcataa cgcccaaagc
    24001 atcaacatcg ccggagtact caggaacatc aaaagaaacc tgaacatggc tctgtgcagc
    24061 gaggttatag atttcatcag gctgaacaat gctgatgata cgaatcagag aagatgagtc
    24121 ggacaggtca ccgtcatgaa gggtgatctt gcccatcaaa tggtcaatgc gagcggtatt
    24181 ggaaatagaa gcacggcgag tgataccgtg aacttcgtag cccttctcta gcaggaaatc
    24241 tgccagatag gaaccgtcct gaccagtaat accagtaatc agcgcttttt tcatgataca
    24301 ttatcctttc ctatgtaagt gataaggcca tataaatggc tcgtaatttc taatactttt
    24361 caagaataca cgataatttt cgatatttca tagaacaatc tacgcacgca ttagcacaat
    24421 attagttttt cgtg
//
LOCUS       DACDLI010000038        24272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDLI010000038 DACDLI010000000
VERSION     DACDLI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.038"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IV353_001578"
     CDS             <1..572
                     /locus_tag="IV353_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9009827.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IV353_001579"
     CDS             576..1217
                     /locus_tag="IV353_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009828.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IV353_001580"
     CDS             1214..2131
                     /locus_tag="IV353_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9009829.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IV353_001581"
     CDS             2128..2799
                     /locus_tag="IV353_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009830.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IV353_001582"
     CDS             complement(3340..3828)
                     /locus_tag="IV353_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9009831.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IV353_001583"
     CDS             complement(4029..4295)
                     /locus_tag="IV353_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9009832.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IV353_001584"
     CDS             complement(4295..4558)
                     /locus_tag="IV353_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9009833.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IV353_001585"
     CDS             complement(4551..4823)
                     /locus_tag="IV353_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009834.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IV353_001586"
     CDS             5034..5219
                     /locus_tag="IV353_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9009835.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IV353_001587"
     CDS             complement(5361..7184)
                     /locus_tag="IV353_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9009836.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IV353_001588"
     CDS             complement(7782..10487)
                     /locus_tag="IV353_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9009837.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IV353_001589"
     CDS             complement(10793..11740)
                     /locus_tag="IV353_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9009838.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IV353_001590"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IV353_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9009839.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IV353_001591"
     CDS             complement(12375..12737)
                     /locus_tag="IV353_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9009840.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IV353_001592"
     CDS             complement(12748..13869)
                     /locus_tag="IV353_001592"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9009841.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IV353_001593"
     CDS             complement(13885..14235)
                     /locus_tag="IV353_001593"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9009842.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IV353_001594"
     CDS             complement(14365..15876)
                     /locus_tag="IV353_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9009843.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IV353_001595"
     CDS             complement(16198..17550)
                     /locus_tag="IV353_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9009844.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IV353_001596"
     CDS             complement(17703..18779)
                     /locus_tag="IV353_001596"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9009845.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IV353_001597"
     CDS             18975..20720
                     /locus_tag="IV353_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9009846.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIATFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IV353_001598"
     CDS             complement(21000..21437)
                     /locus_tag="IV353_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9009847.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IV353_001599"
     CDS             complement(21421..22794)
                     /locus_tag="IV353_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009848.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IV353_001600"
     CDS             complement(23116..23604)
                     /locus_tag="IV353_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9009849.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IV353_001601"
     CDS             complement(23626..24228)
                     /locus_tag="IV353_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9009850.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6960 a         5163 c         4487 g         7662 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccacattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc tt
//
LOCUS       DACDLI010000039        24045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDLI010000039 DACDLI010000000
VERSION     DACDLI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.039"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IV353_001602"
     CDS             299..1960
                     /locus_tag="IV353_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9009851.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IV353_001603"
     CDS             complement(2030..2377)
                     /locus_tag="IV353_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9009852.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IV353_001604"
     CDS             2461..3402
                     /locus_tag="IV353_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009853.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IV353_001605"
     CDS             3403..4446
                     /locus_tag="IV353_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9009854.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IV353_001606"
     CDS             4452..5522
                     /locus_tag="IV353_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009855.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IV353_001607"
     CDS             5512..6465
                     /locus_tag="IV353_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9009856.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IV353_001608"
     CDS             6477..7487
                     /locus_tag="IV353_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9009857.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IV353_001609"
     CDS             complement(7772..8593)
                     /locus_tag="IV353_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9009858.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IV353_001610"
     CDS             complement(8690..8968)
                     /locus_tag="IV353_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009859.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IV353_001611"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IV353_001611"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9009860.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IV353_001612"
     CDS             complement(11724..12311)
                     /locus_tag="IV353_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009861.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IV353_001613"
     CDS             12486..13808
                     /locus_tag="IV353_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9009862.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IV353_001614"
     CDS             13963..14739
                     /locus_tag="IV353_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9009863.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IV353_001615"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IV353_001615"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9009864.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IV353_001616"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IV353_001616"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9009865.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IV353_001617"
     CDS             16629..17258
                     /locus_tag="IV353_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009866.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IV353_001618"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IV353_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9009867.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IV353_001619"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IV353_001619"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9009868.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IV353_001620"
     CDS             19445..19981
                     /locus_tag="IV353_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9009869.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IV353_001621"
     CDS             complement(20021..21784)
                     /locus_tag="IV353_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009870.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IV353_001622"
     CDS             complement(21787..23502)
                     /locus_tag="IV353_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009871.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7767 a         4368 c         4866 g         7044 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaa
//
LOCUS       DACDLI010000040        23884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDLI010000040 DACDLI010000000
VERSION     DACDLI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.040"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(54..788)
                     /locus_tag="IV353_001623"
     CDS             complement(54..788)
                     /locus_tag="IV353_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9009872.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="IV353_001624"
     CDS             1115..1525
                     /locus_tag="IV353_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9009873.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="IV353_001625"
     CDS             1518..2219
                     /locus_tag="IV353_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9009874.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="IV353_001626"
     CDS             2219..3805
                     /locus_tag="IV353_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9009875.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="IV353_001627"
     CDS             3824..4045
                     /locus_tag="IV353_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9009876.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="IV353_001628"
     CDS             4042..4803
                     /locus_tag="IV353_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9009877.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="IV353_001629"
     CDS             5183..5692
                     /locus_tag="IV353_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9009878.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="IV353_001630"
     CDS             complement(5767..6306)
                     /locus_tag="IV353_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9009879.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IV353_001631"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IV353_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9009880.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7373..8170
                     /locus_tag="IV353_001632"
     CDS             7373..8170
                     /locus_tag="IV353_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9009881.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8198..8833
                     /locus_tag="IV353_001633"
     CDS             8198..8833
                     /locus_tag="IV353_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9009882.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="IV353_001634"
     CDS             8853..9626
                     /locus_tag="IV353_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9009883.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="IV353_001635"
     CDS             10034..10831
                     /locus_tag="IV353_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9009884.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="IV353_001636"
     CDS             10809..11222
                     /locus_tag="IV353_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009885.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="IV353_001637"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="IV353_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9009886.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQTIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="IV353_001638"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="IV353_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9009887.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="IV353_001639"
     CDS             15999..16559
                     /locus_tag="IV353_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9009888.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IV353_001640"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IV353_001640"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9009889.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="IV353_001641"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="IV353_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9009890.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="IV353_001642"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="IV353_001642"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9009891.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="IV353_001643"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="IV353_001643"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9009892.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="IV353_001644"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="IV353_001644"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9009893.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="IV353_001645"
     CDS             21906..22484
                     /locus_tag="IV353_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9009894.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="IV353_001646"
     CDS             22536..23018
                     /locus_tag="IV353_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9009895.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="IV353_001647"
     CDS             23135..23509
                     /locus_tag="IV353_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009896.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7809 a         4289 c         5123 g         6663 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaaac tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtc
//
LOCUS       DACDLI010000041        23563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDLI010000041 DACDLI010000000
VERSION     DACDLI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.041"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IV353_001648"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IV353_001648"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9009897.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IV353_001649"
     CDS             complement(1174..1869)
                     /locus_tag="IV353_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9009898.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IV353_001650"
     CDS             complement(1882..2598)
                     /locus_tag="IV353_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9009899.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IV353_001651"
     CDS             2928..3533
                     /locus_tag="IV353_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9009900.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IV353_001652"
     CDS             3858..4181
                     /locus_tag="IV353_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9009901.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IV353_001653"
     CDS             4363..5733
                     /locus_tag="IV353_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9009902.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IV353_001654"
     CDS             complement(5863..7164)
                     /locus_tag="IV353_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9009903.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IV353_001655"
                     /pseudo
     CDS             complement(7394..9385)
                     /locus_tag="IV353_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
     gene            complement(9529..9795)
                     /locus_tag="IV353_001656"
     CDS             complement(9529..9795)
                     /locus_tag="IV353_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9009904.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IV353_001657"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IV353_001657"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9009905.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IV353_001658"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IV353_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9009906.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IV353_001659"
     CDS             13139..13702
                     /locus_tag="IV353_001659"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9009907.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IV353_001660"
     CDS             complement(13955..14476)
                     /locus_tag="IV353_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9009908.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IV353_001661"
     CDS             complement(14479..15321)
                     /locus_tag="IV353_001661"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9009909.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IV353_001662"
     CDS             complement(15314..16039)
                     /locus_tag="IV353_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9009910.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IV353_001663"
     CDS             16215..16511
                     /locus_tag="IV353_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009911.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="IV353_001664"
     CDS             17079..18671
                     /locus_tag="IV353_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9009912.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="IV353_001665"
     CDS             18684..19424
                     /locus_tag="IV353_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9009913.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19592..20941
                     /locus_tag="IV353_001666"
     CDS             19592..20941
                     /locus_tag="IV353_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9009914.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21069..21659)
                     /locus_tag="IV353_001667"
     CDS             complement(21069..21659)
                     /locus_tag="IV353_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9009915.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21806..23158
                     /gene="brnQ"
                     /locus_tag="IV353_001668"
     CDS             21806..23158
                     /gene="brnQ"
                     /locus_tag="IV353_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9009916.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6748 a         4743 c         4489 g         7583 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattatcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta
    19561 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    19621 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    19681 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    19741 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    19801 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    19861 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    19921 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    19981 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    20041 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    20101 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    20161 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    20221 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    20281 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    20341 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    20401 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    20461 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    20521 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    20581 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    20641 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    20701 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    20761 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    20821 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    20881 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    20941 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    21001 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag
    21061 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    21121 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    21181 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    21241 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    21301 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    21361 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    21421 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    21481 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    21541 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaata
    21601 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    21661 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca
    21721 tcgttttata tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    21781 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    21841 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    21901 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    21961 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    22021 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    22081 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    22141 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    22201 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    22261 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    22321 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    22381 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    22441 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    22501 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    22561 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    22621 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    22681 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    22741 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    22801 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    22861 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    22921 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    22981 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    23041 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    23101 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    23161 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    23221 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    23281 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    23341 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    23401 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    23461 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    23521 tttataatat agattttaga catatatatg atataaaaat agt
//
LOCUS       DACDLI010000042        23444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDLI010000042 DACDLI010000000
VERSION     DACDLI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.042"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(312..734)
                     /locus_tag="IV353_001669"
     CDS             complement(312..734)
                     /locus_tag="IV353_001669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9009917.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(748..2154)
                     /gene="atpD"
                     /locus_tag="IV353_001670"
     CDS             complement(748..2154)
                     /gene="atpD"
                     /locus_tag="IV353_001670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9009918.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2177..3079)
                     /locus_tag="IV353_001671"
     CDS             complement(2177..3079)
                     /locus_tag="IV353_001671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9009919.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3093..4649)
                     /locus_tag="IV353_001672"
     CDS             complement(3093..4649)
                     /locus_tag="IV353_001672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9009920.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4672..5214)
                     /locus_tag="IV353_001673"
     CDS             complement(4672..5214)
                     /locus_tag="IV353_001673"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9009921.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5201..5725)
                     /gene="atpF"
                     /locus_tag="IV353_001674"
     CDS             complement(5201..5725)
                     /gene="atpF"
                     /locus_tag="IV353_001674"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9009922.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5816..6031)
                     /gene="atpE"
                     /locus_tag="IV353_001675"
     CDS             complement(5816..6031)
                     /gene="atpE"
                     /locus_tag="IV353_001675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9009923.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6084..6803)
                     /gene="atpB"
                     /locus_tag="IV353_001676"
     CDS             complement(6084..6803)
                     /gene="atpB"
                     /locus_tag="IV353_001676"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9009924.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7105..9948)
                     /gene="rnr"
                     /locus_tag="IV353_001677"
                     /pseudo
     CDS             complement(7105..9948)
                     /gene="rnr"
                     /locus_tag="IV353_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(9951..10697)
                     /locus_tag="IV353_001678"
     CDS             complement(9951..10697)
                     /locus_tag="IV353_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9009925.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10809..11045)
                     /gene="secG"
                     /locus_tag="IV353_001679"
     CDS             complement(10809..11045)
                     /gene="secG"
                     /locus_tag="IV353_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9009926.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11163..11441)
                     /locus_tag="IV353_001680"
     CDS             complement(11163..11441)
                     /locus_tag="IV353_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9009927.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11554..12522)
                     /locus_tag="IV353_001681"
     CDS             complement(11554..12522)
                     /locus_tag="IV353_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9009928.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12737..12781
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12805..13779
                     /locus_tag="IV353_001682"
     CDS             12805..13779
                     /locus_tag="IV353_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9009929.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13784..14359
                     /locus_tag="IV353_001683"
     CDS             13784..14359
                     /locus_tag="IV353_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9009930.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14426..15565)
                     /locus_tag="IV353_001684"
     CDS             complement(14426..15565)
                     /locus_tag="IV353_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9009931.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(15965..17263)
                     /gene="eno"
                     /locus_tag="IV353_001685"
     CDS             complement(15965..17263)
                     /gene="eno"
                     /locus_tag="IV353_001685"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9009932.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(17379..18134)
                     /locus_tag="IV353_001686"
     CDS             complement(17379..18134)
                     /locus_tag="IV353_001686"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9009933.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(18238..19428)
                     /locus_tag="IV353_001687"
     CDS             complement(18238..19428)
                     /locus_tag="IV353_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9009934.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(19538..20539)
                     /gene="gap"
                     /locus_tag="IV353_001688"
     CDS             complement(19538..20539)
                     /gene="gap"
                     /locus_tag="IV353_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9009935.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(20580..21617)
                     /locus_tag="IV353_001689"
     CDS             complement(20580..21617)
                     /locus_tag="IV353_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9009936.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(21820..23154)
                     /gene="rpoN"
                     /locus_tag="IV353_001690"
     CDS             complement(21820..23154)
                     /gene="rpoN"
                     /locus_tag="IV353_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9009937.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            23214..23285
                     /locus_tag="IV353_001691"
     tRNA            23214..23285
                     /locus_tag="IV353_001691"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23247..23249,aa:Arg,seq:ccg)
BASE COUNT         6299 a         5014 c         4242 g         7889 t
ORIGIN      
        1 aaaagtcttg tcgcagtccc ttattccaaa taaacggtgt tcaatcatct gaccctcggt
       61 attagctcaa aaaacggcaa aatatgagga actattttcc atttgtttct ctaatactca
      121 ggacaaaacg ctgatttcac aacctccgat atacggtgtt ccagaagtag cttcttcgaa
      181 aaataagctg agatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatgtc
      241 ttgaagctga ccacttctgt cataacctct ttttactgtt cttaaacacg gataaacatc
      301 cgcatctcat tttatgagtg cttagatact ttgatacgat tgattgctcg gtgcaacgca
      361 acagttgccc gtttcaattc gttcgtatct tgttttgctt ttgcttcttc gatttggcgt
      421 tctgcgcgtt gttttgcacg ttccgcacga gatacatcga tatcacgttc acgttccgca
      481 ctatcagcaa cgattgaaac aacattatca cgaacttcca tgattccgcc attaacagcg
      541 atccaatcga cgtgggtatc tgaatctgtt cgttttacac gaacttcatc aatcgtcaat
      601 ggaacgatga ttggtgcatg tttaggcaaa atcccaagct caccagctgt cgtacgtgca
      661 acgacgatag ctgcatggtg atcatagacc aaaccggcag gagttaccac atttactgtt
      721 aaatattgat ccatggggca cccctttcta gtagcctagt gttttcgctt tttctacgac
      781 gtcttcgatt cggccaacac ttcggaaagc ttcttcagga agatcatcgt atttgccttc
      841 caagatttcg cggaatcctt tcactgtttc ttctacaggg acatatgaac ctggaagacc
      901 agtaaattgt tcagccacgt taaagttttg tgacaagaag aattggactc tgcgggcacg
      961 agaaactaac actttttctt gatcggataa ttcatccata cctaagatag caatgatatc
     1021 ttgtaattca cgatatcgtt gcaatacgtg ttggacttct gttgctactt tataatgttc
     1081 ttctcccacg atttcaggtg ctaaagcact tgaagaagaa gccaatggat ctaccgctgg
     1141 ataaatccct tgttctgtta atttacgttc caagttggtt gtcgcatcca aatgggcgaa
     1201 tgctgttgcc ggcgccgggt cagtgtagtc atcggctggc acatagattg cttggatcga
     1261 tgtgatcgaa ccttttttcg tagatgtgat acgttcttgc aattgaccca tttctgttgc
     1321 caatgttggt tgataaccaa ccgcagaagg catccgtcct aacaaggctg atacttcaga
     1381 tccagcttga gtgaaacgga aaatgttgtc gataaacaac agtacatctt gaccttctac
     1441 atcacggaaa tattccgcaa tcgtcaaacc agtcaaagca acacgcatac gtgcacctgg
     1501 tggttcgttc atttgtccga acaccatggc tgttttttcg ataactcctg aatctttcat
     1561 ttcataatac aagtcattac cttcacgagt acgttcacct acaccggtaa atacagaaat
     1621 accaccatgt tcttgggcga tattatggat cagttcttgg atcagtacgg ttttaccaac
     1681 accggcacca ccgaatagtc cgactttccc accttttaaa tatggggcaa gcaaatcgat
     1741 aactttgatc cctgtttcca aaatttctgt acttgtactt aattcgtcaa aggctggtgc
     1801 ttttttatga atttcgcttc tttccgcatc ttctgggaaa ggtgtttcta aatcgatcgt
     1861 atctcctagt acgttgaata cgcgacctaa tgtttcttta cctacaggaa cagagattgc
     1921 tttgcctgta tcaatgactt ccattcctct ttgtaaacca tctgttgatt ccatcgcaat
     1981 ggtccggatc actccgtccc ctaattctaa cgctgcttca agaacaactt ttgatttatt
     2041 ttcgtcattt ttataaacaa ctaatgcgtt gttgatatct ggtaaggatt ggtctaaaga
     2101 aaattccacg tcgacaacgg gaccgattac ttgaacaatc ttgcctgaac tcatcttttt
     2161 tcctccaatc tcaatactat tctagtgccg aagctccggc aacaatttcg gtaatttctt
     2221 gggtaatagc cccttgtctt gcacggttgt aagaaaccgt cagctcatca atgatattag
     2281 cagcattatc agttgctgtt ttcattgctg tcataccagc agcatgttct gctgttttgg
     2341 cgtcaacgat cgcaccgtag atcaaacttt cagcatactg aggcaataac tgagacaaga
     2401 tttcttcttt tgaaggttca aaaatatatt cttgttcgaa agtagttgct tcttctggat
     2461 ctaagtctga gattggcagc attttttcta cacgaaactg actagttagc gaattgatat
     2521 gatggttata acatacgtat aattcatcga atacttcgtt ttggtacata gttgttgcca
     2581 tatttacgat tttgcggact tcatcaaagc ttggctgatc agatagattg cgcagctcat
     2641 atgctagatt gataccgcgt gctttaaaga agtctgcacc agttccgcca attgcgatca
     2701 tcacgtaatc atcgggtgat tggtggtctt cttctaaaat agacatcgtt tgtttcaata
     2761 tcgagctgtt atatccaccg actaaaccgc catctgcagt gatgacgata taacccgttt
     2821 tcttaacagg acggctgagc aacatgctgt tgtaattgat atcccctcta ggattttctg
     2881 aggcaatatc atttaactga gtagcagtca aatgcgtaac gatttctctt acttttgttg
     2941 cataaacctg aaacttctgt gaagaagctt cagattttgt caacttagaa gcagacacca
     3001 tctgcattgc ccgcgtgatt tggctcgttt ttttcgttga agcgatacgt gtcttgattt
     3061 catttaatga agcacccatt catctcacct cactatgcat tttgaatgga tttcaattta
     3121 tcttctgtag aggaattatt tccttttgta gctaagaaca tgtctttata ttcctgaatc
     3181 gcactgttca acgcttcttc ttctggaaga tcttttgttg tacggattgt ttcaaagata
     3241 tctgaatgat tggtatccag atattcgaat aattcatgtt cgaagtctaa aatactgtct
     3301 acagggatac tatctaagaa tccatgtgtc aaagcataca aaatcactac ttgtttttca
     3361 acagctagcg gtgcatgcaa tttttgtttc aagatttcta ctgtacggcg tccacgattt
     3421 agttttgctt gtgttgctgc atccaagtca gaaccgaatt gtgtaaatgc ttctaattca
     3481 cggtaacttg ctaagtctag acgtaaagtc ccagcaactt ttttcattgc tttgatttgc
     3541 gcagaacccc ctacacgaga tacagatagt ccggcatcaa ccgctggacg agtacctgcg
     3601 tagaacaagt cgctttctaa gaagatttgt ccatcagtga tcgaaataac gtttgttggg
     3661 atataagcag agatatctcc agcttgtgtt tcaacaaatg gtaaggcagt cattgaaccg
     3721 ccacctaaat catcacttaa ttttgctgca cgttccaata agcgtgagtg taagtagaaa
     3781 acatcccctg gataagcttc acgacctggt ggacggcgaa gcagcaagga aagttcacga
     3841 taagcaacag cttgttttga aagatcatca aaaatgatca agacatgttt cccgttgtac
     3901 atgaactctt cacccattgc agtaccagca tatggtgcga tataaagcaa cggcgctggt
     3961 tgagacgcac cggcattcac aacgattgtg taatccattg cgccatattt tttcaatgtt
     4021 tcaacttgtg tacgaactgt agaatctttt tgtccgattg ctacatagat acagatcata
     4081 tcttgacctt tttggttgat gatcgtatcg atcgcaatgg aagttttccc tgttttacgg
     4141 tcaccgataa ctaattcacg ttggccgcgt ccgattggta caagagcgtc gattgctttt
     4201 agacctgttt gcattggttc gttaacagat ttacgttgca taacgcctgg tgccattgct
     4261 tctactggac gagctttgtc agtaacgatt tcacctagtc cgtcaattgg ctgacctagc
     4321 ggattaacta cccgtccaat caaggcctct ccaactggaa cttccatgat ctttcctgtt
     4381 cgttttactt tatctccttc acgaatggac tcgaaatcgc caaggataat gatacctaca
     4441 tcatttgatt ctaagttttg cgccattccg tatgagccat ttgaaaattc aagcaactca
     4501 ccactcatcg cgttttctaa accatgtgca cgagcgatcc cgtcacctac ataagttacg
     4561 gtaccgattt cttcgactga aagcacgttt tgataatttt caatttgttc tttaatcaag
     4621 gcactaattt cttctgcttt gatggccatt cgattcacct acctctttgt actatctatt
     4681 taattgatta cgcatatttt ctaattgcgt gcgaatgctt ccgtcgatta ctcgatggtt
     4741 agcttcaaca atcgcgccac caataataga agaatcaacg atttgtttta attcgactga
     4801 ttgatagttc attgtttttg caacattctt ttctaattgt attcgttgtt cgtctgaaag
     4861 tggaactgct gttgtgacac ttcccaacag caaaccttga tgttcatcat atcgacgttc
     4921 atattcttcg atcatgaaca aaagatcatc cattcggtta tacgcataaa caacttctaa
     4981 aaagttttta acgattccat catagccact aacaagcttg tccatgattt ctcgtttttc
     5041 atgaggttct agacgaacat cacttaacat attacctaat ccaggtactt gttcatagat
     5101 ttgacgtaag cttacgagat cttgataaat ttcttctgct ttatttgaat cgatcgccag
     5161 ttcaaataaa gctttcccgt aacgtcttcc tactgtatat ttatctagtt tcatttgaag
     5221 aacctagacc ttcgatatat tgattgatta atgattcgtg catttctgga gataattctt
     5281 tgttcaagat tttttccgcg atttgaagag atagttctgc aacatcgtct tttacagagt
     5341 tcagcgctga atcacgttct acagtgatat cagcttgggc tttacttttt agacgagctg
     5401 cttcttcttg agcatccttc aaaatatttt ggcggcttaa ttctccactt tctttcgcat
     5461 ttttgatgat atcagctgca tcagaacgag aggctaacaa ttgttgttcg cgttcttgtt
     5521 ccattttcgc tgagttgatg cgagattgtt ctgcagaatc caaatcattg gcgatcttgt
     5581 cttcacgttt tttcaaaata tcgctgattg gtccccaagc aaaatgcttc aagagagcca
     5641 gtagtatcaa aaatgagccg ctgacaacga tgacatttcc taacatcgca ttgccaactt
     5701 ctgcaattgc caattgattc agcataggcg agccttcctt tctttgaagt acttctagtt
     5761 ttcatgtaaa aaagcagtaa caaaccaaat tgtttctgcc ttaatactga ttcaattaaa
     5821 caccaaaaac tagaatcaag gcgacaacta cccctaagat aggaacagct tcgactagag
     5881 ctaccccgat aaacattgtt gtacggaatt gaccagacat ttcaggttgg cgagccattg
     5941 attcgattgt tttagagata acttgaccgt taccataacc ggctccgatt gctgctccca
     6001 tgattgcgat tgctgctgcg atataattca taattgtttt cctcctaaat tagataaata
     6061 gtagtttgtt tttaaaacga atattattct tccacttcaa tcttatggct catataaacc
     6121 atagataaag taacgaagac aaatgcttgg atgcttccga taaacaagga gaatgcgatc
     6181 cagaccatct ctaatggaat tgctaacggc aatgagaacc agcctaagct tattaccatt
     6241 tttgcaatca aggttagcaa aacttccccc gcataaatat tcccataaag acgtaaagca
     6301 agtgttaata agtttgtaaa ttcttcaatc agtttcatcg gaaacataaa actatatggt
     6361 ttcaagaacg aatttacgaa atatcctttg aagcctaact ttttcacccc aaataagtga
     6421 gtaagtaaga tcattataaa tgccaaagtt aaagtaacaa acgggtctgc tgtcgggctt
     6481 ttccaaagtg ttgtttcgtt aggtaaaacg atttttgtta ctaaaccgat gttgtttgca
     6541 acaaaaacaa ataaaaacaa tgtaaatgcc aacaaatgga aattgctcaa ttcttttcta
     6601 ggcagattat ctgttacgat tcctcgacta aaatcaatga cccattctat cgcattttgt
     6661 ttgcctgttg gtttcaactt aagatttctt gtaaaaaagt aagctaagaa gaaaacaatc
     6721 aaacaggtca gtaacaccat tatacaaact gtaccatcaa accaaattgg tccaatgtgg
     6781 aacgttagcg agcgttcatc caatccattt cacctctttt ccccgaaatt cacataaaat
     6841 caattcatgt gcgtatctgg ttttttccaa tcggtttcta accacacggt atcataccac
     6901 catgaaacag aaaattcaaa acattttcat gaaaattaga cactttttta tttcagtcaa
     6961 aaacgaagcg aatttttctt tatacacggt gctcctgatc taagaaaaaa cctatgataa
     7021 tcatgtttta tcagattaaa tgctaacaaa ttttatcatt aagtaaaagc aaaagcaccg
     7081 aatatgcgaa aaaggataat gaaattatct agagaaattt tttaatgttc gatcaatttg
     7141 tcgatcgata tcttttcgtt tcaaatcttc ccgtttatca taagattttt tccctttcgc
     7201 cagaccaatc aataccttgg catagccgtt acgaatatag acttttaaag gaacaacagt
     7261 gattccagta tttttcaatt cattttcaag ccggatgatt tgttttttgt gtaataataa
     7321 ctttctcgtg cgcaacggat catgattaaa aatatttcct tgttcataag gactgatatg
     7381 aacattatgc aagaaagctt ctccgttgcg gacgcgaata aatccatctt ttagattgat
     7441 ccggctgttt cgtatcgact tgatctcggt tccttgcaag accatccctg cttccatcgt
     7501 gtcgatgatc gaataatcat ggcgagcttt cttgttttgt gcaattaatt ttccctcgcc
     7561 ttttggcata gctgtttcct ccaactcatt ttattttttc tttttcttct ttttaacacc
     7621 tttgtaaaat ggcttcttcc cttttttctt accgaacgtt tgcttactat cgctatatcg
     7681 tttgttcttc ttgccatttc tttctgggcg ttttcctgct tgttttcctt cgcgttttcc
     7741 tgattttcca tttcgtgttt ttggccgttc caacgctgta acctcttctg cggaaacaag
     7801 ttcaaaatct actgctcgtt catctggatc agctttgact acttttactc gcactttctg
     7861 tccgatttta aaagtcagac cagttctttc acctacaaga gctaaatgat tttcaacaaa
     7921 atggaagtag tcctgtttca attcattgat atggatcaat ccctctactg tattcggcag
     7981 ttcgataaac aggccgaatt tcacaacaga gctgatgatc ccatcgaatt cttcccccac
     8041 tttgtccatc atgaattctg ctttcttcat ggaatctact tcacgttctg tttctactgc
     8101 acgacgttcc atcttagaac tatgatctgc aatttcagga agttccgtag cccacgaact
     8161 ttgattgctt tcacttttat cttcactgta agaacggatc agacgatgga cgattagatc
     8221 gggatagcgt cgaataggag atgtaaagtg tgtgtagtaa tctgccgcta agccataatg
     8281 cccaaaattg tcttctgaat agcgtgcttg ttgcatgctt cttagcaaca tagtattgat
     8341 aactgcagct tctggtttgt cttctactcc ttcgatcaca tgctgtaaat cttttggtgt
     8401 gatcgttcct ttcgttcctc ggaccagaat gcccaaagct gaagcaaaat caaagaagcg
     8461 ctgcattttt tcttcttttg gctgttcatg gattcggtaa ataaacggca gatttaattt
     8521 attataatgt tcagctaccg tttcattggc cgcaagcatg aaagattcga tcaagcgttc
     8581 accaattcct ctgcttctta gctggatatc atttgggtgt ccttcagcat caacaaggat
     8641 ttttgcttcg cgatcctcaa aagaaatcgc gccacgtttg attcgcatcg tttcaaggat
     8701 tcgatgcaat tcacccattt cttggaacat aggtacgagc tgagcatagc gttgcatcgt
     8761 ttcttcgtct tgatcttcta agatttgatt gacagctgtg taggtcatcc gttctgttgt
     8821 ttggatcaca cttgggaaaa tatcatgaga gactacttgt ccatttggat caatctccat
     8881 ttcgcaactc atggtcagcc ttggaacatg gggattcagt gaacagatac cattcgataa
     8941 acgttgcggg atcatcggaa tcactcgatc agtcaaatat acacttgtcc ctctttcata
     9001 tgcttctctg tccaattgac tgccttgtgt cacatagtaa gaaacatctg caatgtgtac
     9061 tccaagaaaa taattaccgt tatctaattt tttaaccgta actgcatcat ctagatcttt
     9121 tgcatcttct ccatcaatcg taacaatgag ctgatttctt aaatctctac gtccagttat
     9181 atccttttcg ctgatttgat ctggaacttc atctgcttca gacaatacat catcaggaaa
     9241 tttcgtaggg ataccattag ccacgacaat tgagaggata tccatgccag ggtcattctt
     9301 atgaccaatc acttgcttga ttatcccttc taaactagtc gcataccctt tttcagggta
     9361 atgggtcagt tcgatcagca caatacttcc gtcaactgga cgaactccct ctgctgctgc
     9421 atatacttta aattggttga gctttctatc tttcgggata acaacgccgt aaagatccgt
     9481 ttcttgtatt tcttcttctg aataagcaac gaattcccct gctaactgag tagtcgcccg
     9541 gtctcgaatc tcgactactt tcccctctgc tccgcgatcc atatctgtat ttgctcgctt
     9601 catgatatcg attgctacgg tgtctccatc cattgcaaaa ccagtcgctt cttttgggat
     9661 atagatgtca tcttcttcgg gatcaatcgt tacaaatcca aatcctcgtt catttgctcg
     9721 aaatgttcct tcgatcaaga ttggttttaa aggaagtttc acttttcctt ttttattgaa
     9781 aactatactc tgttctcttt ccatttgagc aatcgtttgt actagtaatt taaaatcatc
     9841 gcttttttgg aaccctaaac cttcagcgat ttcttccata gaaaaactgc gtttgcgact
     9901 gctttccatg aaaaagagaa ttttctcttt tatcgtttct tttgtcatat ttattcctca
     9961 ttccaaggta atttttctaa aaattccaag acgtcttttt ctaattcgcg tcttgctgta
    10021 ccaactgtaa tcacatgacc actttcagga taccatcgaa gtgtcactcg ctgacggctc
    10081 aacttgcgtg cagtttcaaa tacgccatta gggtcgatca tttcatcttt cccagcttga
    10141 gctaaaaata tcggctgttg gatatctgac aacctacttt ctacgattgc tgcctgatct
    10201 tgtatatcca ttaattgttg ttcagctaac gagcggtaag caactgcttt ttcattgatt
    10261 tctttctctg atttacctgc tgtcttcagc acttgacgta cgtatttttc aaagttttca
    10321 ggcacgtttg tttttacagg agacaaaggc gaacagaaaa aaccgccgcc aatcactgat
    10381 tcttcttcta gtgctcgtac agcaaagatg ccgcccattg ataatcctaa tacggcaata
    10441 tcagaaaact gctcagactg taaaaaatgg atggcttttt tagtgtctgc ccaccagctt
    10501 tctgcttttt cttgcaaaat atcatatggc tccattgttc cgtgtccttt aaaaagcggc
    10561 gcataaactg tataatctgc tttttccaaa aatcgtgcga gcattcgaac atcgtttgga
    10621 cttcctgagt aagcgtgaag cagcaaaacc gctcgctttc catgttttgc ataaaggggt
    10681 tttggtaatt gtctcactcg tctcacctct ttcgcctcat tttaccatag tttctttcat
    10741 aacttcttct taagcgttct ctttgcaata aaaaaagccg cccgccgttt tgcgagaggc
    10801 ttattcaact acttcgaaga taaatatgct aggacaaata ataagatcat ccagatagct
    10861 cccataactg cagttgatcg ctgcatgaca gcttcaaagc cgcgagcttt ttgtttgccg
    10921 aatagttgat ccgcaccgcc ggtaaatgca ctcgctgcac tattttgctt gcttggctgc
    10981 atcataatcg caatgaccat aacaatcgat aaaacgatca cgattcccaa aattaaatta
    11041 tacatatcgc agcctccttc ccaaaagata tcgtctcttt aactttatca tagataaaag
    11101 tccgtgacaa ctctatataa atggaataac agctgaaagt tcaaaacttt cagctgttat
    11161 tcttatgact gacgtaagac gtcttgatat tctggtttct tttcaaactc tttttttgca
    11221 taagggcata aaggaatgat ctttttcccg tctcgtcttg ctaattctac tccagtatac
    11281 actagctttt cagctaactt ttgaccacga tatgctggat ctacaaatgt atggtcaatg
    11341 atcatgatgt ctgggcccgc atctgaccat gtcatttctc cgatctcttg ttgttcatca
    11401 ttgaataaag caagacgatt tttttcttct ttgatttcca tatgccgcac tcctcaaata
    11461 actgatattt ttttattcct accttatttt agcagtctcc tgatctataa acaattattt
    11521 atcctcgaat tacttacaaa aaagaagaga aaattaattc atcgttattc tttgttcaat
    11581 aaatgaaaaa atcactgtcc aataggtttt cggatctttc atcgatgctt ccaagtgttc
    11641 tgcttctgga acaagcagca gtgctttatc ccctgccgta gcattttgaa tcgtgtacgc
    11701 tgcttctgta gggacaaaat gatctctttc accatgaatg accaataaag gcagatggtt
    11761 gctgccaagc tgtctcgtag ctgatgcttg ctttaaggag taaccggcgt atttctgggc
    11821 ataatgattc gcatatctca tgaccatttt tttaggaaag acagtgattt tacttaacat
    11881 atatcggaat tctgcataaa cagaaacata tccgctgtct aaaataaatc ctttgaccgc
    11941 tgatggaagt ttttcaccac ttgccatcat gatcgtagaa gctcccattg atccgccgtg
    12001 taaaataatg gacgcattcg gctgttcatc taagatgagt tggatccagg caattaaatc
    12061 tagccgatct agccatccca ttccgatgat ttctccttca ctttctccgt gcgcacgtaa
    12121 atcaggtaca agaacgttga atcctttctc tgcatatctt ttacctatat gagacatgtc
    12181 tcttttccct gtgcttcggt aatcatgtac gcaaatgacc catttattct gcttatttgg
    12241 atggatgaac atctgaccag ctagtttcaa cccgtcataa cttttgattg ttttgggtgt
    12301 tccctgctcc caaaagcttt cacactcttc ttctaatttt tgtcgttttt tttctactga
    12361 cgtgactgca gcagggtcca tcaaacgatg accagtgtct tcataccagt catatcctct
    12421 ctgtactccc gctcttacta atttctttgc ataaaaataa gaaagaaaat ggtaactgat
    12481 ccctataatt atcagaataa gaatgaaata aaaaataacc attgtacacc cgccgctttc
    12541 tgccaaacta taatctaact attatccaat gtaccgatcc ataggattct ttattttcgg
    12601 cacacgtaaa ttccatttct ttttcattgt atcatttact taaagttctc ctcaatagat
    12661 ttacttgtaa gagagcattt acattcgcaa taaaagccag tatactaatt tcgtgcatta
    12721 taatcaagac gctcgtcttg gactggttcg gaaacttccc agaataaaaa actaagtatc
    12781 tctaaagaaa ggagattgga aataatgcga aaagtaatta ttgattgcga tccagggatc
    12841 gacgacactt tagctttatc attagctgtc aaatcccctg atatcgaagt agttgctatt
    12901 accgtagttt gtgggaatgt ccctgcagat ataggtacgc agaatgtgct gaaatgcctg
    12961 gaacgctgtg atcgtttaga cattcctgtt tatcaaggaa tggaaaaacc tttgaagcaa
    13021 cctttcatca gtgcacaaga cactcatggt atggatggtt taggggaaac aaatttcccg
    13081 atgatcttga gaaaacaggc agaacctctc catgccgttg attttttggc tgattatttc
    13141 aaagaaaaga caaatacatc agtcattgct ttgggtcctt tgaccaatat cgcttttgct
    13201 ttgaaagtca acccaaatat tgggaaacat atggatcgtt ttgtctcgat gggtggaact
    13261 tacaaaagcc atggtaactg ttcgccagtt gctgaataca attactggtg tgatcctgaa
    13321 gcagcttctt atgtattcga gaatctcaaa caaacgatag aaatggttgg gttagatgtg
    13381 acaagagaaa tcgtcttgac accgactatc ctcgaatact gctgtcaaat gaatcctgaa
    13441 gagggagagt atctaaaagc gatcactcgt ttttattttg acttccactg gaaacaagaa
    13501 agaattttag gatgtgtcat caatgatccg ttagctgttg cttattttat cgagggggcc
    13561 atctgtgaag gtttcaaaag cttcactgct gtggaaactc aaggaataag tcgtggacaa
    13621 acattggttg atcgttatga attttggcag aaacctgcta acagtaaaat tatgacaaaa
    13681 gtagatactc gtcttttttt ccgaaagttt ttaaccgttc ttttaaatgc acaagaagaa
    13741 acgattatga aagatttgga aaggttaaag atgggctaac aaaatgaata aaaacaaaat
    13801 ggcaactcgc gtacttacag tgatggcttt gtgtattggg atcaattata ttggaggaac
    13861 gattgcttta tggcttcgtt tgccaatcta tctcgattct atcggaacga tttttgctgg
    13921 tgcattgctt gggcctgtac ctggaatgct gacaggattg tccagcggta ttttaagtgg
    13981 agtgactaca gatatttttt ctctgtatta ttcgccaatc caaattctca caggcttgct
    14041 tgctggattg attttgccaa gatatctcgg caaagataaa aacagcttga aactgttatt
    14101 actcgctttg attttgtcgg tacctggaac gatcgtttct tcgatcatta ccgttcagtt
    14161 atttggcggg atcacctcct caggatccag tatgatcgtt cagctattac atgggttagg
    14221 tttaagtcag acaacaagtg tgatcttggt acaggcaggt acagactacc ttgatcgttt
    14281 gttgtctatc gtcgttgttt cattagtcat cactatgttg ccacaacgct tgatatattc
    14341 aaaaagaaaa ataggctgaa taaacagagc tggattagca aaataatgct aatctggctc
    14401 tgttttttca tttattaaaa aaatcttacg ctaagtagga taaaataatt ccttgatatt
    14461 tttcaatcat atccaaataa ttatcgattt caacatactc atctacttga tgaggcaaag
    14521 taacgacacc aggcccaaag acgacaaagt cgaaggaatg gttagctttc gtaaattcgg
    14581 ctgcatccgt tgttgctgcc gctccaacta aaggtaatgg ctggctgaat tgttgctgaa
    14641 tgcaatgaat caaaggagaa tctggatcag ctttgacagg tattttgttg taatcgatca
    14701 tcaattccaa atgatagtct gtctcttgat tcaattcatt gacgattgat tgcagcaatg
    14761 caatgatttt atcatttggg tattctggaa tcgatcgaat gttcccttgg agctgcgcat
    14821 ggcttgggat gctgttgact tgattcccgc cactgatcaa tgtaacatta tggattgttt
    14881 ttcctaggac aggattctcg attgtttcag cgaggtgatt catttcagca tttgctttcg
    14941 taatgaattc atttaggtga ttgattgcat tataaccttg gtctggcata gagctatgcg
    15001 cttcttttcc gtgcgaggtg actgtatagt tgattgatcc catgtgcgta tacatcaaac
    15061 tgtaattagt tggctctccg atgatcaagg catctaagtc atctacatag cctgctttcg
    15121 tgagctgttc cccacctagt tcacccactt cttctccgac agtggctaat aatttgactg
    15181 ttccgttaaa tggttttcct gactctttca gttcgatcat ggcaatcacc attgctgcca
    15241 agccactctt catatctgta gcaccacgtc cgtacaaacg gttcccatgg atttctgctg
    15301 caaagggtgc ataggtccaa gacgattcat caccagccgc cacgacatcc atatgcccag
    15361 ataggcctaa cacttttcct gattggccct tttggtaacg agcaatcaaa ttatctctac
    15421 catctctgta agaaacaatc tctcctgtta tgtcatgctt tgccaaaagc ttattcaaat
    15481 aggctgctac ttcaccttcg tttccattca ctgatttaat tcggatgatc tcttgtaaaa
    15541 tcgcgatttt ttcttctttt ttcatctaat cctctccctt gccatttctt tttattgtat
    15601 ttgagtgtgc tcgaattttc aaataggagc aaccaaagta aaaatgaaga aagagagcca
    15661 ctttatgaaa aatatgctgc tacttccctc ctattttatt tatacgagag gacttgctca
    15721 tagattccta ttctactgtt ctatccttaa aagctcttat atgatgctag ttattgttag
    15781 ctttcctttt tcaaggatga tcttttcttg taccattctt tgtaaacatc atatacagaa
    15841 gctagttgca aaaaaaactc tctacctgct gatatttcag caagtagaga gttcgattac
    15901 ttaactaagt gatcatactc gactatcttt caatagtcga tcatgtatct tcattagaaa
    15961 agaattattt gttttttaag ttgtagaaag atttcaaacc tttgtattct gcaacttcac
    16021 caagttggtc ttcgatacgt agtaattggt tgtatttagc aatacggtca gtacgtgata
    16081 gagaaccagt tttgatttgg ccagcgtttg ttgcaacagc gatatcagag attgttgaat
    16141 cttctgtttc accagaacgg tgagatacaa ctgctgtgta gccagcttct ttagccattt
    16201 cgatagcttc gaaagtttct gttaatgtac cgatttggtt cactttgata aggattgagt
    16261 tagcaattcc tttttcgata ccttcagata atttttgagt gtttgttacg aacaagtcgt
    16321 caccaactaa ttgaactttg tcgcctaata cgtcagttaa tttcttgaat ccgtcccagt
    16381 cgttttcgtc taatccgtct tcgattgaga tgattggata tttagaaact aattcttcgt
    16441 agaatttgat catttcgtca gttgtttttt cgccttcgcc tgaatcagct agtacgtaaa
    16501 cacctttttc tttgtcgtag aattctgaag aagcagcatc catagcaaga acaacgtctt
    16561 taccaggtac atagccagct ttttcgattg cttcgatgat tacttcaaaa ccttcttcgt
    16621 ttgaaccaag gtttggagca aatccacctt cgtcaccaac agaagttgct aatccgcgag
    16681 ctttcaagat tgaagctaat gcgtggaata cttcagcacc catacgtaga gcttctttga
    16741 atgttggagc gccaacaggc atgatcatga attcttggaa gtcgattgag ttatcagcat
    16801 gtgatccgcc gttgatgatg ttcatcattg gtgttggcaa tacttttgtg ttgaatccgc
    16861 ctaggtagtg gtataaaggt acttctaggt agtcagcagc tgcacgtgca acagcgatag
    16921 aaacacctaa aatagcgttc gcacctaatt tacctttgtt aggagttccg tctaaagcga
    16981 tcatagcttt gtcgatagcc atttggtcac gaacatcgta gccaatgatt gcttcagcaa
    17041 tgatgttgtt tacgttgtcg acagctttag ttacaccttt accgccataa cgagctttgt
    17101 ctccgtcacg taattcaact gcttcgtatt caccagttga agcacctgaa ggaaccattc
    17161 cgcggccaaa agctccgctt tctgtgtata cttctacttc gattgttggg ttaccacgtg
    17221 agtctaagat ctcgcgagcg taaacatcag taataattga catgttttgt ctctcctttg
    17281 agtttgtttt ttcctaaggg atttccctca tatacgattt tagttaattt tgctcattag
    17341 tgcaaacatt tcttgcattt ttcgcaaatt ttcaacaatt attttacagc gtctaacaat
    17401 gacaagaatg aatctgcttg caagcttgcg ccaccgacta aagcaccgtc aacattttct
    17461 tttgccatgt attcagcaat attttctggt tttacagaac cgccgtattg gatacgaact
    17521 gcttctgata cttcttttcc ataaagtttt tcaactgttt gacgtacaac tccacaaatt
    17581 tcgtcagcga tttgtgcgtc agctgattta ccagtaccga ttgcccagat tggttcataa
    17641 gcaataacca tgtttgacac ttgttcagct gaaagatcag ctaaaccagc tgtaatttgt
    17701 ccttcgatcc attcagcagt tttgcctgct tcataggttt ctaaagattc gccacagcat
    17761 aggattggtg tcataccatt tgcaaagata gctttcgctt ttttattgat gtcttcgtct
    17821 gtttcatgga aatattcacg acgttctgaa tgtccgatga tcacataatg aacacctaga
    17881 tcagcaagag ctgctggtga agtttcacca gtgaacgcac ctgagttttc ccagtagcag
    17941 ttttgtgctg agatttttag ttctgtacct tcagctgatg tcattaattc ttgtaagaat
    18001 aatgcaggag atccgatgac tgaatcaact ttgtcatttg aagggatttt gttttttact
    18061 gcttcagcaa attcttttgc ttctgatgca gttttgttca ttttccagtt acctgcgata
    18121 ataggtttac gcattgagga acacactcct taatttattg aaaaatggct agggagtcat
    18181 gaaaactccc ttcacctttt gatcgattac tcgtcagaat tgagcaaact tggtcgttta
    18241 tttatcgttg attgctgcta gtcctggtaa ttctttacct tcaagcaatt ccaagcttgc
    18301 gccgccacct gttgagatgt gtgtgaattt gtcagcaaat cctaattgtt ctgctgctgc
    18361 ggctgagtct ccgccaccga tgattgttgt tgcgccttct aagttagcga ttgcttcaca
    18421 aacaccgatt gtacctttag caaagttact catttcaaat acacccatag gtccgttcca
    18481 aacaactgtt tttgcacctt gtaatgtatc agcaaataat tttactgatg ctgggccgat
    18541 gtctaaagcc atcaaaccat caggaatgtc tccttcgatt acttgtgttt caacatcatt
    18601 tgaaaattct ggtgcacaga cattgtcgat tggcaagaca agtttgtcgc ctgctttttc
    18661 gattaattct tttgctagtt ccactttatc agcttctact agagagttac cgatcttgat
    18721 tccttttgct tcgtagaatg tgtaagtcat tccgccaccg atcaagattt tatctgcttt
    18781 aggaatcaag ttttcgatta cgccgatttt atcagaaact tttgcgccac caaggattgc
    18841 aatcattggt cgttttggtt cttctactgc ttcaccgatg aacttgattt ctttttccat
    18901 caagaatcca gcaactgttg ggataccagt tgaagcgata cctacgttag aagcatgtgc
    18961 acggtgagct gtaccgaaag catcgttaac aaatacatcg cctaatgaag cccagtattt
    19021 acctagttct gtatcatttt tgctttcttt tttaccgtca atatcttcaa atcgtgtatt
    19081 ttcgaatacg acaacgtcgc cgtcattcat gttgcggatt gcttcttcta attgttctcc
    19141 gcgagtttca ggtacgaatg ttacttcttt acctaataat tcgcctagac gttttgcaac
    19201 tggagctaaa gattttcctt ctttatcttc ttctgttttt acacgtccaa ggtgagagaa
    19261 aaggattgct tttccgcctt gttctagaac gtagttgatt gttggcaatg ctgctttgat
    19321 acgagtgtcg tcagtgatca cgccgtcttt caaaggaacg ttgaagtcaa cacggacaag
    19381 aacttttttg tcttttaaat cgatatcttt cactgttttc ttagccattt tatttcctcc
    19441 tgaataaact tattatcaaa aaaagcggaa gaagcgcgat tgcttccccg cttttaaatg
    19501 tacatgtcta taatttacac ttgtacgttt gtctatctta taagttagcg aagtattcta
    19561 aagtacgtac taattgtgca gtgtatgaca tttcgttgtc gtaccaagca acagttttaa
    19621 ctaattgttt gtcgccaact gtcattactt tagtttgtgt agcatcaaat aatgaaccgt
    19681 aagtcatacc aacgatatct gaagaaacga tttcgtctgt gttgtatccg taagattcgt
    19741 ttgaagcttc tttcattgct gcattgattt cgtcaacagt tacttctttt tcaagaactg
    19801 ttactaattc tgttaatgaa cctgttggta caggaacacg ttgagcagct ccgtctaatt
    19861 taccgttcaa ttcaggaatt actaaaccga tagctttagc agcacctgtt gagttaggaa
    19921 cgatgtttgc agcagcagcg cgtgcacggc ggaagtcacc tttaggatga ggtccgtcta
    19981 gagtcatttg gtcacctgtg taagcgtgga tagttgtcat taatccttca acaacaccaa
    20041 atttgtcgtt caatgtttta gccataggag ctaaacagtt agtagtacat gaagcacctg
    20101 agataactgt ttctttacca gttaatgttt cgtggtttgt gttataaacg attgttggaa
    20161 catcgtttcc gccaggagca gagataacaa cgcgtttagc accagctgtt aaatgttttt
    20221 cagctgctga tttagaagtg aagaaaccag tacattctag aacgatatct acgcctagtt
    20281 cgccccatgg taattcttct gggttgcggt tagctaatac tttaacttct tttccgttta
    20341 cgttgaatga accttcatga acttcaactg ttccgttgaa acgtccttga gttgtgtcgt
    20401 attttaacaa gtgtgccaac attttagcat ctgtcaagtc gttgattgct actacttcga
    20461 ttccatctac atcttggata cgacggaatg ctagacgtcc aatacgtcca aaaccattaa
    20521 tacctacttt aactgtcatt taagatttcc tccttatgaa aatcaaaaaa atatttattt
    20581 taaagggtta ccccttttaa aatctcattt gcagcagctt catcagtgat gagccacgtt
    20641 tgttttggtg catttttcat ataagctcga attgctttgg cttttgattt tccgcccgcg
    20701 attgccatga tgatcgggac tttttccaaa tcttgcaatt ctatgccgat tctagggacc
    20761 ttataaacga cttcaccttt ttcattgaaa aagtagccaa aagattccgc aacagcattt
    20821 gcttgtttca gcatgactaa ttctttttca gtcatcttac gacgtgctgc catgtgtaat
    20881 gcacgcccaa tactatgaat aacacaattc gcttcactaa tcaagtttaa gacttcttgg
    20941 atagatggct cattgagtaa agaattgtaa gttgccagac ttaattgttc cggaacataa
    21001 agtgcacggt ggttaccgtt tgctttagca gccattcttg cactgacaga atttgcctgt
    21061 acagttatcg cttcaccaat cccgcctctt gcagggacaa acaggttatg ccgtttctta
    21121 ttttccagat tagacaattg ttcggctacc acagccatag tagttcctcc catcacagcg
    21181 atgatattct cgccttctgg aagtagtcgg tctagggctt cgctcagcgc ttcgccgaat
    21241 gcatcagcca ctttggtctg ctcttcgctg tttcctgaaa caacgataca gcgttggtta
    21301 ccaaaatatt ctgccaactg ttgttcggtt tgatgcgtac ctaaaagctg atccatgaag
    21361 ttttcaaggc tttgataaac ttcttcgccc ttagttgtca gcgtcatacc gctttttgag
    21421 gtgtcgatca aattcagatt tttcaaaaga tctgtttctg ttcgcaatac tcgttctgtg
    21481 atccccatac tttccgataa acttcgacgt ccaatcggct gcatccaata gatatttcgt
    21541 aaaattttat agcgttcttg caacacttcg ataatatctg gtgcaacaga ttcaatcatt
    21601 tttatttcat ccagcatagc gatctcctta gttggacact ttacgtccaa tgtagactta
    21661 aaacgaccca tatacgataa aaaaagaaat taactattcg aatttcacat caataaaggg
    21721 aaatctcaac ctcacgactg taagtctaac agttttgctc tcgtttttca actaaaaaga
    21781 ctagaaaaag tgaaatactt tttctagtct tttgactgat tagttatcat aacgttttct
    21841 ttttgatgaa ctcgggattc ctaataaatc acgatatttt gctactgttc gtcttgagat
    21901 cgctatctca tcttttttca gcaattcaac caatttctgg tcagacaacg gcttgttttt
    21961 gtcttcttga tcgactaatt cctgcagctt tttcttcgct gtatctgctg aaaggtcttc
    22021 tgtctgttct gtactattcg taggaattcg tgtagtgaaa aattttttta attcaaatac
    22081 accgaagtct gtttccaaat atttcccgtt gactgcacga ctgaccgtgg attcatgcac
    22141 atctatctca gttgcaactt cctttaacgt aagaggcttg attggacgtt ccttgtcgat
    22201 aaagaaagct ttttgccggg aaacaatgac ttgagcaacc cgcaaaattg tatccccacg
    22261 ttgtaggatc gttttcttca accattcgaa ttcctgtttc cgttctttta aataattttc
    22321 agtttcttta tctgcttgtt gtgacatttg tttgaagtaa ctctcttgaa agcgtatatt
    22381 tggctgattt tttcggtttg agataacttg tacattttta tcgtcgtcca ctaatacccg
    22441 gacatcagga ataatgtaca attcgctgct acggtcgaaa atccggccag ggcttggact
    22501 caacgtttgt aaatagtcga atatctgctg aacagcatgt agatcaacag aaaaacgttg
    22561 tgcgatttct ttccattttc gctccaccag ttcctcaaaa cattcttcca acacgatata
    22621 agctaattcc ggcgcataat catctcgttc tgtttgaagc atgagacatt cttgcaaact
    22681 acgtgcacct actccagctg gttctaattg ctgaatcaga gtcaacgcat cgagcatttg
    22741 gataggcgta gctccaatct cttttccggc ttcttctaaa ctaatcgtca aaaatccgtt
    22801 caaatcgatg tattctacaa gataaagaac gattttcctc aaaaaagtat ctcggtaatt
    22861 caaatggact tgttcgatca atgaatcaaa taaagaccct tttgtatcag gaatctgatt
    22921 taggtaattt gtctcttctc cttcataaga agatgaagaa gcttttatgt aatctggctg
    22981 ccattccggc tcaacaactt ccaccaatgg attttccatt gcttgattct caacgaatgc
    23041 caacagctcc tcactgttat attgtaaaac ttggatggac tgttgcaact gttgcgtcat
    23101 ggctagtttc tgtgtctgct gctgtttttg tgaaaaattt tgctcaaatt tcattttttt
    23161 gtccaatccc cttgtaattt tttatctgtt tagttacaat tatcccagtg cttgcgtcct
    23221 tagtgtagtg gatatcacgt aagattccgg ttcttgagac gggggttcga ttccctcagg
    23281 acgcgttcta ctgctatttt agcataattt acgaatagtt gcttctttat ttttaagtga
    23341 aaactgtccg ccaaagaaaa ttggtggaca gttttttgtt gtacctgtat agatattgtt
    23401 aagcctttgc aagtaaggta gttatgtgtt caactactta ttaa
//
LOCUS       DACDLI010000043        22827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDLI010000043 DACDLI010000000
VERSION     DACDLI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.043"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..214
                     /locus_tag="IV353_001692"
     CDS             <1..214
                     /locus_tag="IV353_001692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009938.1"
                     /translation="GDSSSDLKRATEFIKKFYSSKALQNVSREILEIIFSEQKDLLLG
                     IQTVRIIWEGVQYLPSETLTIDIETN"
     gene            273..1418
                     /locus_tag="IV353_001693"
     CDS             273..1418
                     /locus_tag="IV353_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9009939.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGINPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKQDTNETFQEIYELWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGKFRIKEITVKHVQKILNQWAKMTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRNQLEILFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALNWSDIDFDSNTVTINKTLSQTKYGYKISSPKTKNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(1503..1808)
                     /locus_tag="IV353_001694"
     CDS             complement(1503..1808)
                     /locus_tag="IV353_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9009940.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1833..2264)
                     /gene="ruvX"
                     /locus_tag="IV353_001695"
     CDS             complement(1833..2264)
                     /gene="ruvX"
                     /locus_tag="IV353_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9009941.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2261..2527)
                     /locus_tag="IV353_001696"
     CDS             complement(2261..2527)
                     /locus_tag="IV353_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9009942.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(2782..3507)
                     /locus_tag="IV353_001697"
     CDS             complement(2782..3507)
                     /locus_tag="IV353_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9009943.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(3868..3938)
                     /locus_tag="IV353_001698"
     tRNA            complement(3868..3938)
                     /locus_tag="IV353_001698"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3904..3906),aa:Gly,seq:ccc)
     gene            4055..4807
                     /locus_tag="IV353_001699"
     CDS             4055..4807
                     /locus_tag="IV353_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9009944.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4823..5302
                     /locus_tag="IV353_001700"
     CDS             4823..5302
                     /locus_tag="IV353_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9009945.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(5553..7133)
                     /locus_tag="IV353_001701"
     CDS             complement(5553..7133)
                     /locus_tag="IV353_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9009946.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(7126..7869)
                     /locus_tag="IV353_001702"
     CDS             complement(7126..7869)
                     /locus_tag="IV353_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9009947.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(8045..8821)
                     /locus_tag="IV353_001703"
     CDS             complement(8045..8821)
                     /locus_tag="IV353_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9009948.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(9069..9905)
                     /locus_tag="IV353_001704"
     CDS             complement(9069..9905)
                     /locus_tag="IV353_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9009949.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(9916..10845)
                     /locus_tag="IV353_001705"
     CDS             complement(9916..10845)
                     /locus_tag="IV353_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9009950.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11037..11390
                     /locus_tag="IV353_001706"
     CDS             11037..11390
                     /locus_tag="IV353_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9009951.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(11464..12120)
                     /locus_tag="IV353_001707"
     CDS             complement(11464..12120)
                     /locus_tag="IV353_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9009952.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            12346..14280
                     /locus_tag="IV353_001708"
     CDS             12346..14280
                     /locus_tag="IV353_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9009953.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(14366..15589)
                     /locus_tag="IV353_001709"
     CDS             complement(14366..15589)
                     /locus_tag="IV353_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9009954.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(15583..17481)
                     /locus_tag="IV353_001710"
     CDS             complement(15583..17481)
                     /locus_tag="IV353_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9009955.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(17475..18593)
                     /locus_tag="IV353_001711"
     CDS             complement(17475..18593)
                     /locus_tag="IV353_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9009956.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(18676..19509)
                     /locus_tag="IV353_001712"
     CDS             complement(18676..19509)
                     /locus_tag="IV353_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9009957.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(19502..20419)
                     /locus_tag="IV353_001713"
     CDS             complement(19502..20419)
                     /locus_tag="IV353_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9009958.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(20646..21770)
                     /locus_tag="IV353_001714"
     CDS             complement(20646..21770)
                     /locus_tag="IV353_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9009959.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            21953..>22827
                     /locus_tag="IV353_001715"
     CDS             21953..>22827
                     /locus_tag="IV353_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9009960.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTG"
BASE COUNT         7243 a         4466 c         3735 g         7383 t
ORIGIN      
        1 aggagattca tcttcagatt taaaaagagc aacagaattc ataaaaaaat tttattcttc
       61 aaaagcactt caaaatgttt caagagaaat attagaaatt atcttttcag aacaaaaaga
      121 cttactactt gggattcaga cagtaagaat aatctgggaa ggagttcaat acttacctag
      181 tgaaacatta actatcgata tagaaacaaa ttagttaaat cagatttatt tcctatcaaa
      241 agcacgccac gcaactgata ggaggaaaaa caatggcaac aattaaacaa tatgaaaaga
      301 aagacggaac caaagcatgg ttgtttcaaa cttaccttgg aatcaatcca ttaactggta
      361 aagaagtccg tactacacgt agaaatttta aaacaaaaaa agaagctcaa atcgagctta
      421 atagactact tgtagacttt gaagagaacg gtcttaaaca ggatacgaac gagacttttc
      481 aagaaatcta tgaactttgg tatgaaagct ataaaaccac tgtaaaggaa gtcactcaat
      541 taaaaattga gatcaagttc aataaatgga ttcttccaaa atatggtaaa ttcaggatca
      601 aagaaatcac tgtaaaacac gttcagaaaa tattgaatca atgggcgaaa atgacagatc
      661 aatataaagt cctacattct actgtaagcc gtattttcaa atacgcaatg actttggggc
      721 taattactaa aaacccctgt gaacttgtaa tcatgcccaa tagaaatgtt gaaaaaaaga
      781 aagatactgt caaaatttat tcacggaatc aactagaaat tctcttccaa tacttggaat
      841 caaaaagaag tacctataga gcagattacg ataaaacgct acttcgcttt ctgtttttta
      901 gtggttgtcg aattagtgag gctttagctt tgaactggtc agatattgac tttgattcaa
      961 acaccgttac tataaacaaa accttaagtc aaacaaaata tggatacaaa atttcaagtc
     1021 caaaaacgaa aaatagctat ggttctctat ctttagacaa taaaaccatt aattggctaa
     1081 agaaatggca aatcaatcaa aaaagatata tgttaaccct tggtattact gatccaacaa
     1141 tgattttttg tggaatctac aaggaaatca ttactaaaca cgctatttat tctcgtatga
     1201 tcacaatctg caagaattct ggtattccat ttctaggaaa ccatgtaacc agacacactc
     1261 atgctagtat gttgttagag tcaggagttt ctatgaagga agttcaagaa cgccttagac
     1321 actcatcaat caaattaaca atggatactt atgctcatct aactaaagaa accaaagaaa
     1381 aaaccgttga aaagcttgca gaacacctaa atttctagcg gaacgtattc aatctcgtat
     1441 tcaaaacaaa acacaaaaaa gctgaaccct tatctaccaa gggttcagcc atcattttat
     1501 gtctattctt cttcttcagc catgaatgta ttgaatactt cttcaatcat gtcccattct
     1561 gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc gatgtatgca
     1621 taagcttgta attctacatc ttcatcttct gaaacaccag caggatataa aagaacatag
     1681 ttacgtccaa attcttcttg tccgtcaact gttagtagga tttcatataa tgtttcattt
     1741 ccttggtcat ccaccaatgt gatgtgttcg tgtccttcat ggtcgtggga atggttatgt
     1801 tctgtcataa gattacctct tttcttttat tattaaaata attgcttacc tgagccgtct
     1861 aaataatttt gtaagatcat cacagctgct aatttatcga tgactttttt tcgtttggct
     1921 cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt taatcgttca
     1981 tcttgataat caactggcaa ggagaatagt tctttgatct tttctccgta tgacatggaa
     2041 gcttctgccc ttggtccgat ggaattgttc atatttttag gtaatccaac tacaaaccgg
     2101 tctacttcat attgtgctac taattccttc aaccgatcaa agccaaactc ttcttctgct
     2161 tcattgatcc gaatgatctc tactccttga gctgtccagc caaaggggtc actgatagca
     2221 acacctactg tcttagaacc gacatccaag cccattactc tcataaattc actccatgat
     2281 ttgagaggta gtccttgaca attacttcca tgatctcatc gcgttcgtga cggcgaatca
     2341 aatttcgagc atcacgataa cgaggaatgt aggccgggtc tccagaaagc aagtagccga
     2401 caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca atcgctaatg
     2461 tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt tcatctgtaa
     2521 aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc tacaacctta
     2581 ttttactcga agaaagggaa aactacaaac aaatattcca aacttccgaa taaattaaga
     2641 aacactattt gtatgttttt ttcctttttt ctgtagcttt atcaaaagta ctcttttcat
     2701 aaagtatacg ctatatttct ttgaatgtat gtttttacac aattttttca taatattcat
     2761 aaaaaattcg aatagctgct actaattttt tgtttttgtt gcttgtcctg tctcataatt
     2821 gatcgtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt ttttgtcttc
     2881 aatgctttct gcttccaatt gcactcccct ggctaccagt tcattcccct caaaatgagg
     2941 agtgacacgg tagcgtacat gatgatttgt ttctttgata tatgccgcaa tatcattttc
     3001 gtgagcaagc atgtatggag cattcaacga acgtgtccct gtcatcaagt ttttaatgtt
     3061 gttattttga ccagtcagct ggaaaccaat taaatggctg cgattatata accattgccc
     3121 gtcacttaat ttcttttgtt tccaacctgt cgggtcaata tacaaaggct ctctcttttc
     3181 agtagggaac aaatcttttc caagcatagc attggcttgt cctactctgt tcaatttatc
     3241 aatatttgaa tagctttccc atgttccttt ggaaaggcta agatcctctt ttgtaaattc
     3301 cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg gcacatccgc
     3361 tacttgagaa gcttgtggtg tggaatcaaa aatagttgta atctgctgaa agatatctga
     3421 atctttttgt gtggtggaaa caccgaatgc ccctgcaatc acagcagcga cgatccccac
     3481 gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagc tccctaaatc
     3541 ttaacatatg ttcccctata aataaatact agaaagaaat agaaacagca aaaaataaaa
     3601 cctgttttaa gatggcttaa aaatacaaaa aagagacgga ttcctcgtct ctaatcagtc
     3661 accactctat ctgtaaggta tttattatta ttcaaaaatt ttcttcatac tcaagacaat
     3721 acactgattt cacaaccgct tggtaaacgg tgcgcaaaag cttgcatctg cggtaataag
     3781 tccatccaag ttaaaaacat aagaagttgt ttttgcttgg ctgttcttat tacttgttgc
     3841 agagcagctt tttcacaacc tattttaagc gggtgacggg aatcgaaccc gcgacatcag
     3901 cttgggaagc tgggatttta ccactaaact acacccgcaa tacaaaaaca gtatacgcct
     3961 cttttattcc ttcctcaagt ttttttattc tttacgcttc tggtatgttt tgtagtaaaa
     4021 taaaactaat caatcaatta ttggagaaga tgctatgacg ggaaaagaat tagatctgtt
     4081 gctggacact tgtttactcg ctgggaaaat catgatggaa agcaatgcgg agatgtatcg
     4141 ggtagaagat acgatgagtc gaattgcttt agcttctgga aatttccgtt tggtcagcta
     4201 tgtcactcaa accggtctct ttattggtct tgatcgtacc tcgaccatcc gaatggaaca
     4261 aattaccaac cgttcgatta atttagaaaa agtagtaaaa gtaaatgatc tttcacgtaa
     4321 atatgttgct tcagagctaa cattagaaga actttactgc gaactgcaag caatcgaccg
     4381 agaccgttct ttctttcctg tctggttgca aatcgttagc gcggcagtga tcagtggaac
     4441 aattatggtg ttatttggcg gaaagatgac cgatttacct gtgactttag tcattggagg
     4501 attgggatac gcagcttatc tatatagttt gaaatttttt agaatcaaat ttttatcgga
     4561 atttttatct tctttattga ttggcatagc cgctatctac agcgtacgtt ttggcttagg
     4621 aagcaatcag gatctaatca ttatcggatg tgtgatgcct ttagttcctg gagttcaaat
     4681 tacgaacgct atccgtgatt tgctagctgg acattatgtc tcaggagttt ctcgcggaac
     4741 agaagcgatg atgactgcta cgatgatcgg ttttgcgatt gcttttattt tccagctttt
     4801 ttattaggaa aggaacacaa gtatggggac gatatttatt cagttttctt ttagtttttt
     4861 agcgactgcg gcatttgccg ttattacaaa tgtacctagg cgttcattga tttgttgtgg
     4921 tctttctggt gcttttggct ggatgatcta ctggatctgt gttcaaatag gcggatctgc
     4981 tgcttttggt tcattgcttg gttctttagg agtagcattc atcagcgatc ttttctctaa
     5041 acgtttgaaa atgcccgtaa cgattttcaa tattcccggg atggttcctc tagtccctgg
     5101 tggtttagct tatcaagcgg ttcgaaatct cgttactgaa tcctatcgag aagcggctca
     5161 ttatgcggtc caagcaatta tgattgctgg agcaatcgct ttaggtcttg tattatcaga
     5221 agtctttaac cataacatcc gtaatttcaa agtaaagcgt gacgcaatca tgatgaaacg
     5281 aaaaaaacgg ctttttaaat aaacgaagtg taacaaagaa aaggttgtga aagagacgtt
     5341 cagccttgag cgatgacaaa atatctcaca aacattgcga caagtaaccg caggagcaat
     5401 aagaagtaat tatgagtatt aaaaaaagtt ttgtaaaaat agcttctcac atttttacat
     5461 aaaattgact taatactgag aaactgactt ttgaacaccc tctatctgta aaaaaggggc
     5521 tgcgacatga tttttgtcac ggcccctctt ctttattcaa attttttcca aaacgttcgt
     5581 tgataataaa cgatccatag agcggaaagt attagaacta aaccgatcac aggaatattc
     5641 actaaccaaa atggataaaa caaagccaac attccgtata agacgataag aataatactg
     5701 atgatcaatg aacccatcat agttgcgact aaacccaaac ttcctgaacc tctgttaaat
     5761 aattggttaa tattggtcca gttcagcagc agcaaccgat aatctctagc gaaataacgt
     5821 agtgacaata agtagcagcc gaataaagaa ccaaatagca agctagggat aaaaaacaaa
     5881 ggcagctgaa aagacagtcc gcttaataat gcgatcccac cagttaaaaa actctgcaat
     5941 atccatccga atctgaactt ttcttttaaa tattggttca tagagattgg caaagaacgg
     6001 ataaacagaa aattttcttg atccaaagaa ataagattgc tgataaaaga agtctgatta
     6061 actgttaaga aagccaatgc gatccctacc aggaaaacaa caccgattaa cttattatct
     6121 aaatcagtta attttatttc gcctcctagg gcaaaagcaa gaataaaaat gattggagtt
     6181 aacaaggaag aagaaagaac ttgcataatc aaattaggtt cttttactaa ttgagcattg
     6241 taactaaata aaagctgatg cacattttgg ttctttttat gggttctttt tattttgaac
     6301 tcactgtttg aatcagtcag ttgctctgta agctttggta ctatcagctg ttttacagca
     6361 aggaaaaatc ccagagccgt gatagctagt attccccaac ccaataaacc ttgttgagaa
     6421 agtggctgac tggcagcatc atagaatggc aataatatcg aaatcggatg ccgatctgtt
     6481 aactcatttt caatagtccc cgtttgatga ttcatccaca tgattccagc aaatgcgatc
     6541 agcatcgagc tgccaagcaa gatactagaa actgtttttc tatgtttttt aaacactgct
     6601 gttcgagcca aaccaaaaac aatcaaacta gccagacaaa aaagaataat caaaaataga
     6661 gaaaacaaaa ggattgagat aatgatagta agcaaaatcc ctagatcagc tcgccatccg
     6721 gttaaaataa acatgactag aagcggaaaa acaaaaggaa tgatggtcaa agcaactacc
     6781 aaaatctttg ctgaaaaaac ctgttcttgt ccaaaaggca gaggcaaaaa tacttgcagg
     6841 tcatttcctt caaaaaagat attataaatg actgatatac tttgagttaa acctaacata
     6901 ccaaaaagtg ccacgtaata agtaaagaaa cctggcaatt taacaaagtt catagcaaac
     6961 atagcaaatc cgtaaatcag taaaaaaatc agccctgaaa aaagattttg agacaaaaga
     7021 tagatagtta atcgctttcc cttttttcct ttctctcttg ctttattcgt gagctgtgga
     7081 ttaacatata aaagattaac tcgaattaat tctaataatt gtcgcttata catacggtcc
     7141 atctcctgtc gcttgccttc cagccatttt caaataaatc ttttctagcg tttcatcagg
     7201 tgagccattc aataattcac taactgcccc ctgataaatc aattcacctt tcttcaaaat
     7261 agccagttta tcgcataact gttgtgcggt atccaataca tgtgtagaaa aaatgactgt
     7321 ttttcctttt gcggcatgag ccttcatcat ttctttcaaa tcaaatgctg cttgaggatc
     7381 aagtccttgc aaaggttcat ccaataccca aatatctgga tctggcagca acgtgccgat
     7441 caaaatcgtt ttttgacgca ttccatgtga aaaacttgcg attgtttcat tttgatgact
     7501 atacatatca aaaagcgctg tcaattctgc taaacgcttt tctttttttt gtgtgtctaa
     7561 ttcataggca gctgaaatca agtcccaata ttctccagcc gtcaattgta aaaaaatatc
     7621 tggggaatca ggcacataac cgattttttg tttgatactc aatcgatttt ccgtcaattt
     7681 cattccatct actaaaatcg ttcccgatga tggttcaata atactaacta aacttttaat
     7741 cgtagttgat tttccggcac cattatgtcc taaaaatcca aaaatttccc cttgtcttat
     7801 cgagagagac aagtctttta atgcttgttt tttcccgtaa cttttactta cattgatgaa
     7861 ttcaatcatt tctgtactcc ttttgtttgt tattactttt tattgtaccc ttcgttattg
     7921 gtggagtaaa tgtcaaaaag agtgcataac aaaaaaggat agaaacgact aggctgctac
     7981 ttgggttgtt ctcttctaca ggaaaacact cttctaacag gtaagatatg agtctttgtt
     8041 ttctttaaaa tgattcattt tctatctttt cttgcataaa attctttagt ttatcaatac
     8101 ttctctttcc catctctagt tgttctccat ttgcaaatgt tactatttcg tttactctgc
     8161 taatagatgt aatgttttca gtattaatga tataagcttt taaatcttta cataaatact
     8221 catattcgag tgatttttta tattttttaa ttgtgccgtt tacgagaatc tcttcatctt
     8281 tcaatactag gcctatttta tttttataac ctggcatgct cattagataa aggatttcat
     8341 cgtaatggat atagtgatcg ctattaccat aacggacagt tgtccacctt tttttcgtag
     8401 catgttgaac aggatagttt tctttttctt tcaataagta actttggatg ctttttttat
     8461 ttttttcata agtgaattct tttatcaaat aaccactagg cgtaatcttt cgattaataa
     8521 cagttattgc tttattcgga tgagacgtta gataaacaat tttacacttt tcattctttt
     8581 ctctaagcat ttcgcctagt tcgataccgc tataataggc gttcaaatcg atgtctagga
     8641 aaaaaatatc tgaggaatag atttcattat ttttaatgaa ttcaacaaat tgtataacgt
     8701 tcaatccctg aacgatctca aatttccaag gacttaataa aattctggat tcttccaaaa
     8761 tttctactag cattttttca aaaaccaaac tatcatccaa aataaatata cgcgttacca
     8821 taaaaagtac ctccactcga tattttacta tcaaaatcat ttcatacaac ttttcaacat
     8881 acatgttagg aataaattgt aattctcttc acaatctttt ccaacacctt tgaaaccgat
     8941 atcaaatact ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa tacatcatgc
     9001 aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt ctgagctatg tttatttatt
     9061 ttgttctttt aaaaagaacg atacacatac tgatttttcg gagcagagac tttctcagcg
     9121 tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt gataatagtc tgattggatg
     9181 gttccttcta ttttatacca ttcatcattt ttaaccgtta ctccttccgg catatgcgtc
     9241 aacaaaccaa agactcctga atctgccaca caatggataa tgccgaatcg gaacaagaag
     9301 atatctgtat tgctatcgtt cggtgtttgg taaacaaatc cttcatagct gatttttttg
     9361 ccaatgaatt cgctagggta attataaatt agctccataa cttctaaata gttttcatct
     9421 gtgatcttga tagtttgctt atccccatac ttttctttca actcattcat ttggtctaag
     9481 tagtctgatt tgttgaaata aatactggta tccggcttta agtattgtgt ctgcatctcc
     9541 ggatccccta ctgattcttt acttactggg aaattaaatc ctttggcttc tacgatcgta
     9601 gtgtcgagac taactgttgg aaacaacgtc cccacgatta ctggcaaagc tagcagtaca
     9661 taagcgattc cacgttgata aggttttgac aacccatggt catgatcgtg ctcgtgtgcc
     9721 ttttcttctg cttttccgtt tttgacccac aaatacagct gaactaaagc caaaagagca
     9781 gataaaatca ttgaaagaat agctaagtag cgataatgga cattgatata ttgatcgagt
     9841 ttgccagaaa cttgcaagta catcatcaat tcgaaatacc cagataaaat caaaaaccgc
     9901 agcatatttt ccctcctaaa taaataatgt gaatccaata acgctaaggg caatcagtcc
     9961 gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc atcatcagta gatttttgat
    10021 atcgaccatt gggccaaaga ccaaaaaggc agctacaggt gctgtgccga acagacttaa
    10081 caaagaagat cctataaacg catctgcttc tgagcaaaga gacatcgtaa aggccaataa
    10141 cagcatgatg ataatggcta aaatctttgt tgagccgagc gttagcataa ttctagttgg
    10201 taaataagtc tgcatcgcag ctgcgattaa tccgccaatg atcaaataac gacctgtatc
    10261 aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt tgatggatca gctgattttt
    10321 tggttcattc ttctcacttt gttcttcttc atggacatga tgatggtgat tttctgctac
    10381 tttagacgtg attttttctt tcaatacagc gtctttttga aaataggcaa gccagacacc
    10441 aatcacaagg gcaacaaaaa agctgcctat cacccgccag cttgccatct gccaagaatt
    10501 gccaaatgcg ataaaagtgg aaaaaatcac gattggattg atgatcggag cagtcagcat
    10561 aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa cgatcggtac
    10621 gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa
    10681 gatacgattt tcaggaagcc atctcttcac acgttctggg gtcaaaaata cttgtaaggc
    10741 gcctgagata agacagccta ataatacgaa aggtaatgct tcaataacga tcgataagaa
    10801 aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt tcctcctttt
    10861 tatttctagt tcattcgctt tgcaaatata cgcttttatt caaaaaaaac aagttgctct
    10921 gtaaattttt acacaactct tattttttac caaagcactt caattgactt tttctgttat
    10981 tcccattaaa ttaaggtctc atacatatca cttctatcat aaaagggaga ataaaaatga
    11041 aacaaaccac taacttgact gaagttcaag acatactgca acagtcatcc gttgccgtca
    11101 tttatctttc gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac
    11161 ttctcacaca ttatgatatt ccggcacttc atttggatgc atttgaaaca ccagaagtag
    11221 ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag
    11281 tctttcggca agcacggttc atcgacttta aaaaaatccg ttatctacta gatgaactga
    11341 cagcagaaga tgattccctt tcttatgaag aaatcttccg aacaaaataa tcgttgtcca
    11401 aacagaaaaa agcgattgaa caagtgaaag ttttgccttc atttgttcaa tcgcttttta
    11461 tttttattct gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa
    11521 ggtttgcaat tcattcgtca aatcaacatt ttggatcaat acatctgacg gagcattcaa
    11581 tcgagcagcg gtaaagttca agattccttt gattcctgct tccgttaact gattgactac
    11641 ttcttgtgct ctttcagcgg gtatcgttaa aattgctact tcgatttgtt ggatcttgat
    11701 ttgttctgac atatcttcca ttggataaac ggggataccg tctacgatgc gcccaacgat
    11761 ttcctcattc acatcaaaag cacaactgat acgtatgcta ttactttgat ggaatttgta
    11821 ttttagtaaa gcacttccca agttccctac accgatcagc gcaacatttg tcaattgatc
    11881 gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct acatcataac catacccacg
    11941 ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc gttgcactat ctacttgaac
    12001 ggcttcactt aattcagttg aagacacttt tttctttcca gcattattta aaatccttaa
    12061 atagcgataa tacaaaggca gtcttttagc tgtcgccttt ggaataatat gatctttcac
    12121 gaaatgacct ccacgtattg tctaattaca tctttgattt ctccatcata gcagtattta
    12181 taggaaaaaa tcaattccat tgctcagata ttgtgaaatt ttattcttta ttcatttatt
    12241 tatcatttct gcaaatctcg cagtataatg gaatttatgg tagactacta attgaaaaat
    12301 agttttttat ttgaggagaa ttcacaaagt agaaaagagg attccatgat attattacaa
    12361 gccaatcaga ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat acatttagaa
    12421 atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa atcgacttta
    12481 ttaaagatca ttgctggaat tgaagcacct gatgaaggaa agatcgctaa aaataaaaca
    12541 gcgactctcg gatacctcgc tcaggacaca ggacttgatt ccaatgaaac agtttggaac
    12601 gagatgctga aagcttttga acaagttaga caaatggaag agcggatgcg cgagctagag
    12661 attttcatta gcgaaggtac gccagacaca cctgcttata gttcttattt aaaagaatac
    12721 gaccggctgc aacacgattt ttcagatcga aacggctatg gttatgaaaa tgaaattcgt
    12781 tccgtattgc atgggtttaa attcgattct tcattttatg atcagaaaat cgatacttta
    12841 tcaggcggac agcgcacacg tttagcttta gctcgcatgc ttttgcaaca ccctgacatt
    12901 ttgattctag atgaaccaac gaaccattta gatatcgaga cactttcctg gcttgagaat
    12961 tatctgcaaa gctatactgg tgcattattg attgtttccc atgaccgtta ttttttagat
    13021 aaagtagtca atgaagtcta tgaattaagc agaagaaaaa tgcgccatta caaaggtaac
    13081 tattcgaaat atctagattt gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa
    13141 aagcagcaaa cagaaatcca taaattagaa gacttcgttg ctcggaacct tgttcgcgcc
    13201 tctaccacaa aacgagcgca aagccgcaga aagacattag aaaaaatgga ccggctggat
    13261 cgtcctcaag gggatgaaaa acaagcaaac tttctattca gtatagaaaa gccttctgga
    13321 aatgtagtct tgcaaatcga agatgccgct atcggatatg ataatgtcac cttatctgaa
    13381 ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc
    13441 gggaaatcga cgttgctgaa atcaatcatt caccagatcc cttttatccg tggaaaagaa
    13501 acgctaggga caaatgtcac catcggctat tacgatcaag gtcaagctga tctgcataca
    13561 aacaagacga tcctagcaga attgtgggat gagcatccta ccactccaga aaaagagatc
    13621 cgaagtgctt taggtgcttt cttattttct ggcgaagatg tagaaaaacc aatccctctg
    13681 ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa caaagaaaat
    13741 tttttgattt tggatgaacc aacaaaccat ttagatattg acaacaaaga agtattagaa
    13801 aatgcattga tcgattatga cggcacactg ctttttgttt cccatgaccg atatttcatc
    13861 aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga
    13921 gactatgatt attatttaga gaaaaagcag gaagaagaag aaatcgccga attgttggca
    13981 caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta ccaaagcaaa
    14041 gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga agaagaaatg
    14101 gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg
    14161 gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag acaagatcaa
    14221 gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata tgaaaattaa
    14281 cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt tttctttttt tattttatca
    14341 aacaaaaatt ccatatttac ctttttcacc aataagaaga ccaattacta gtcattctca
    14401 ctaaagcttc taaataatag taatcacccc aaatacaagg tgtatttacc ccattatttt
    14461 tgggcttatt ataaacagac tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc
    14521 tttctttcaa ttgtcgcata attttccaaa cagcagactg gtataagttc ttttctgttt
    14581 tgtctaatag acctgattct attaattcta ataatccaca aaccatgatc accatactag
    14641 atgtatcttt ttcttctctg ctagaatcat caaagatcaa atcccaataa caaatggtat
    14701 cttctggcaa ccgattcata aaataattgg ctaatatttt gcttaaatca aaatattttt
    14761 gttctttcaa ataacgataa cttaatgcaa aaccgtaaat cccccatgct tggcctctag
    14821 cccaacagga attatcacta taaccttgat gcgtacttcc atatcttggt tttccatctt
    14881 tgacatccat ataaaatgta tgataagttg aaccatcttc tcttactaaa taatctgctg
    14941 cattttgaat atgagcctga gcagcttttt tatatttttg atttccagtt tcttcactcg
    15001 cccaatacaa taatggaaga ttcatgcaac aatccatgat cattctacct tgttgtgaag
    15061 gatcactagg atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta
    15121 atgtttctgc agcatcaatt gaagcttcct tcatttttcg atttcccgtt attttataat
    15181 ctgctacggt tgataaagaa tacaaaaaac caagatcgtg atgattagtg ataatttgac
    15241 agtcaatcct gtggataaaa tctatatctg ctttttcagc tgtttgttga aataaaggat
    15301 cacccgtctt ctcaaataac aaccaattaa ttcctgtcca aaatccttct gtccaaccaa
    15361 tattgtctct taatggataa cgtcctttta taggacactc gcttgggaat tgatactgac
    15421 tgttactgat acgtttgatt aacctctccg tgtgtctgcg acaacgctcg atctcattat
    15481 tccattcttt ttccattaat tctttataac attcatttat ccatttttct ggatgtatta
    15541 tctcttcaaa catcacttta tcaagcattt ccccaccagc ctttagcata attttagtct
    15601 ttttttattg tttttatttt ggatagttag cccggttgtc ccatcaaaat caattttggg
    15661 acgattgttc catacttcta aactataatc ccgatcttga tgagcaacca ctcctgttcc
    15721 gagccaatgg gttccttttc caaatgtacc ctctaatcca ggaataaaag tgttttccca
    15781 tgtcatcaag tttgtattgg gcaaacccat aatagctgtg gattttcgtt taatagtatc
    15841 gtcacttaaa cagataatac ctgaaaagcc agcttcactc accctatagt tttccttgtc
    15901 ttcttctatg attattcctt ctttctcagg agaacattca atagcaaacc ctgtctcata
    15961 tagaacatat tctgtatcta attctacttt gtgtacgcga atctgccagc cttcaaaagg
    16021 aataagccaa gttctgattt ttgcctgtaa agtaggttcc catgtagagg caagtacctc
    16081 tttcttacat acaatgtctc gtacattatt tcgaacgatg aactgtccat aggttttgat
    16141 gctctcattc ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc ctagcattga
    16201 atccattgcc atttctgctt tcaattgatt cgctcgcggt atcgaaaaac caaattgact
    16261 agaataagca aattttaaat atttttcatt ggtcaatgta tggaaatttg gccctaattg
    16321 tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat tggcttctga
    16381 aagaagttta gttccttcac gaataggcat aggcatttct ttagccgtcc aatactcatg
    16441 gtcttcaggt agcgccaaca ataaaaaaat cttattcagc cataatggag ataacgtact
    16501 attatagggt tcagtcaatg tcaattgagg gtaggtataa ccaatattca ggataccttt
    16561 ttcatcaaat atttcttttt gcatccacca tcttatattc cgattgacta accccttgat
    16621 ttcacctaat ttaaaaggca aaagatcggc ccacaacata gctgaccaaa agcttgaaac
    16681 tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa
    16741 atgaatatag tctctagaaa aatcaatagc tcggttctta tatatctggc taatgggctt
    16801 ttctggttct agtacgctat aaatcaaacc ataataatga aatgcaaaag gattataata
    16861 atcttggcga cctcttgaac tgtctcgata ccagccattt tgaagataac aattttctat
    16921 attatcaaat gcttctttca gtgctgattc tttaactgga taaccaaatt tttggataat
    16981 tgttccgaca attatcttaa aaaattgcca attaccatct gcacattcat attcaaaaat
    17041 ttgcgagaac caatgaataa tctggctttt ttctcctgta tctaattttt tccataataa
    17101 ttgttcatga tgatataacg ctaataacat tgcgggcatt tctaccatac gctggtctct
    17161 ttcatgtatt tctccccaat aatcgggatg ggaaggatct gttccagctt ttattcctga
    17221 tagaattaat ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg ctccccataa
    17281 aacccgagag aatccttcca ttcctgcaat tttatttcca tatcctaccc cttgatgtat
    17341 ataatcaatt tgtgcgccac ttgctcgata aaacggctgg agtggttgat ttatcttttt
    17401 aaataagtca aagtaatcct gtctggtcct taatagtggt actggatgtt tcattgtatt
    17461 ttctcctctt tttactacca aatagctaat tccgactgtt ctaatcgaag taagccttca
    17521 ataaaaaaat aatcagaata gatcagtggc acattataag tcagttctct atggtatgct
    17581 tctgctccgc ctccaatgat cccatcttca tccatattcc aatttgcata gtgcttttct
    17641 gtcttttgga gtatattgat agcagcttca tgatacattt ttgatcgtac agaggaaaca
    17701 tatcctgcta attccagaag accgcatgca gcgcatactc cagcactggt atcaatattc
    17761 gaatctttta cagcaaaatc aatgagagga atataaccag ttttttcgat tttctgaata
    17821 aaatttactg ctatcttttc tgctatagct agatagtacg gattttttgt gtaacgataa
    17881 cttaacgcga atccatataa agcccatgca tttcctctgc tccaacaaga attctcattg
    17941 tatccttgcc cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata
    18001 tgcccggttg atccatcttt cctcactaga tacttagctg ttgtatctgc atgctgccga
    18061 gcaattttag agaacctcgg atcaccagtt tccctcgaag cccaatacag taatggtaaa
    18121 ttcatcatac tatcaataat catccaacca ggtttatggt cattccatgc tactaaatag
    18181 ttccctgaaa tattatagcg tccagcaagt accgtagctg catgtaaccc tagttgtcga
    18241 ctatgtttgt tttgagactg ttgataatgt gcaatagccg atggtaagaa taaaaaacct
    18301 acatcatgat gaacgtctga aaaatggtac agggcagatt ctaataattg ttcttgttgt
    18361 attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca taaaatacca
    18421 ctccaaaaac cattggtcca ccagtcgatt cctctgtcct ttattacgtc tgtatatttt
    18481 ccgttgtaag aaatatatgg aattttattc cccattctgt ctaattccgc atctatcttt
    18541 tcagaagcta cattcaaaat ttttcttgac cattccttcg tctgttcatt catggatatc
    18601 ccgccattct tttaattttt atgaatgtgg agttgctcct agaacgaact agatggttaa
    18661 cattcgttat tttttttact tatttgcagc tccaatgtca ttctccttaa atttagactg
    18721 cgcccacaca taaatcaaga ccattgggat cactgaagtt gctaaaatta gcataattgc
    18781 gccataatta attggctcac ctgtactaga aaccgtcata tttttgataa ttaagggaac
    18841 cgtatattta tctttatctg aaagcatgac taatggaaat agatattgat tccaacttcc
    18901 cataaagata agtaaaccac tagtaataat cgtaggtgat aaaatcggta cagcgatttt
    18961 cgtaaaaatc acccactcat tggcaccatc gattctggca gcctccatca gtgctgtagg
    19021 aaattgactg ctatattgtc gcatcataaa cactgaagtt gtactggcaa tcgaagggat
    19081 gatcacacct gcataagtat tcgataatcc taaacgactc ataatgtaaa ataaaggaat
    19141 caatgtggta aaaaaaggca ccatcctaga tagcataact gtattgaaca acagtttatt
    19201 ccccataaaa cgatactttg ctaatgcata acccgccatt gtagaactgg ctgtagctaa
    19261 aactgtactg attacagcaa taacaatcga attccaaata atcaagggat aattatatct
    19321 cgccatgatg gcttgccagt tttttaatag catatcccca aacttgaaat tgattccgcc
    19381 acttaaaatt tcttgattac tttgcgttga agctacaatc atatataaat atggcatcaa
    19441 aaatattaag gcaccaaaca caataatgac gtacgtaaaa attctgcttt gtatcttttg
    19501 tttagtcatt tttatcacct ccgaatttaa attgaatagc cgataatata acagatacaa
    19561 acaccatcgt ccaggcaata gcagaggctt ttccaaaatc aaccatttca aaacttgttt
    19621 tatataaatt gactcctacg accaaagctg aattttctgg tccattggaa gtcccaaaga
    19681 atatatttgg gatattgaaa ttacccaagc cattgatagt ggctaaaact gtggccatta
    19741 aaatgattgg ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc
    19801 catctatttc agcactttcg tatatctctt tagaaatccc ttgaagacca gctaaaaata
    19861 aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat gatcattgat attcgtatcc
    19921 aaaaaggatt tgttaaccac tgtacaccgt taattcctag cgcattcaaa aatccattga
    19981 ataacccagt cggattgaaa aaagtcatga atacggaagc tacagccacc gttgatgtaa
    20041 tggttggaat ataatagaca gttctaaaaa atccttttcc ttttaactgt gctgaattca
    20101 ataaaacagc aaagtaaata gctaatagca tcgttattgg aattgatcct gccatgataa
    20161 tcaaaacatt cgtgtaagat ttaataaaca atggatctcg gaagatagct agataattgt
    20221 caaatccaac aaattcaaat gagtagccaa ttttcataaa actaaaagca aacgagataa
    20281 tgatgggaat caaaatcgtc acaataaaga aaataacaaa aggtgtgacc aatccataag
    20341 cacttttttt atgagacgta tctgttgctt ttttcttgct gttctttatt gtcttgcttg
    20401 gtaacgaaga ttgcatcatc gttaagtcct cctaatattt tttaatcacc agttaatttt
    20461 acgccgtact tatgagcaaa attttctgct tcttttagat atgaattttc aaaatcacct
    20521 gtttgccaat agttatatgc agcggtagtc aaataagacg tagcatctga ataagtggct
    20581 ggtaaaacag tatttgatgt attttcattg attttattca atagttcata gtatttttgt
    20641 cctccttagt agtcaaactt tttctcagct gcttctgttt gataggccaa tgagttagat
    20701 gtagccacgc ccagctccag tgcgttggct aagcattctt catctgtcag cataaatgta
    20761 agaaattgtt gtgccacttg tggatttttg gcatctttag ggacataaaa acttgatcct
    20821 cctgatactg caccttttcc tggattttct tttgaaaaag gaatcagtgg agccatgcgc
    20881 catttgtcag atgcatcagg aaaattcaaa gacatattcg tagcatacca gccgccagca
    20941 acaaacattg cagattcttg aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca
    21001 atatctgagt cgatcatttc tttgattaac tttgcagcat gtttcgcttc tttagtattc
    21061 agacggatat ttccttcttc atcaagcaaa gattgattct gctgggctag catgttgata
    21121 aacaattctt ggtccccaga ttctggcaag gcaattccaa aactaccagt tttttctttt
    21181 aatgcttttg ccacctggat atattcttcc caagaactga tttcttcaaa atctacacct
    21241 atttcagcaa atttttttgg ttgataataa caaagtgctg gggtaaaatc actagcccaa
    21301 ggaataatcg tttcattact cgtttgttga cgcaaaatat tcaatttttg atcataaaac
    21361 tgatcgccat atttgtcata aaatccatat tcatcagcac tataaaaagc atcttgaaat
    21421 ttttccagca cacctgctgc tgaagtgtct gccatggaga taatatccgg catatcttcg
    21481 tgtgttacca attttggggt gatcatttgt actgttgctg ttcccgaagc tacattcaca
    21541 atttttaagt caatatttgc tccagttttg cggttataga tttttgcagc attttcaaca
    21601 tagtttgtca gtttgccgtt tgcgacatat acttctaatg tagtggcttt ttttatcatt
    21661 ggatctgcga tgtttgctgt tacttcttcg ttagtttttc cacatgcact taagctgaat
    21721 aatagggtaa tgtttagcaa agctatcacc aatttgtatc tttttttcat gtctatccct
    21781 tctttctttt caagttctta tataaaacgc tttcacttat gtatattaac ggaattagta
    21841 acacaaatat ttatataaaa aaatatttta cttgtcgttt tggcttttca tttttgttat
    21901 aatagtcatt aattataaat agaaagcttt ttatataaaa agagaaagga aagtggttct
    21961 tttgttaaca aacaaccaat cctccaatct tgtcaaaaaa acactaacct attccataga
    22021 tacccatctt catttatctg ctgctcccta ttttcgacta ctaataaatg aaagcgctaa
    22081 tttatatgct gaatttgcac ataaatctat agaactagat aatccttccc ttttattcat
    22141 tgataaacag caagaagtcc aaatcttttc tactcaaaag gaaacacaaa cttttatcat
    22201 tatccagttt tattttgatg agcaattatt agcacatatt acgggacaat ctttttctat
    22261 agatcaagaa ataacatcta tcattacttt tctgaaaaat gaacagttca aaattgatta
    22321 tctaaaacat agtaagccag ctatttccta tgatcaggaa atcaattctt ttttcatact
    22381 atgccaacag atgagcattc atttgctttc cttattagtt acttattgtc tgcaaaaaat
    22441 tcaattgtct tccttaccaa ggcattatca acaggaactt gctttattga caataccttc
    22501 aaatgagaaa gtcattccta aaaaaaagca tattctttat tcagatgaat tagttgatcc
    22561 aatcatacga tatttagaag aacatctttc agaaaatttt ttgcttgaac aattagccca
    22621 aatttttttt atcagtccag ccagcttaaa aaaacatttt agaaaagtga atggaaaaag
    22681 tgttattagc tattttaaag aactaaaaat ggaaaaagca aaacgtctcg tgctagaaaa
    22741 tgaattaacc tatacagaaa ttgcagatat attaggattt cattcgatcc atcatttttc
    22801 ttcagctttt aaaaaatata cagggtt
//
LOCUS       DACDLI010000044        21814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDLI010000044 DACDLI010000000
VERSION     DACDLI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.044"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            126..566
                     /locus_tag="IV353_001716"
     CDS             126..566
                     /locus_tag="IV353_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009961.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            578..1246
                     /locus_tag="IV353_001717"
     CDS             578..1246
                     /locus_tag="IV353_001717"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9009962.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1310..1933
                     /locus_tag="IV353_001718"
     CDS             1310..1933
                     /locus_tag="IV353_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9009963.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            1989..2375
                     /locus_tag="IV353_001719"
     CDS             1989..2375
                     /locus_tag="IV353_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9009964.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2588..3979
                     /locus_tag="IV353_001720"
     CDS             2588..3979
                     /locus_tag="IV353_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9009965.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4590..4958
                     /locus_tag="IV353_001721"
     CDS             4590..4958
                     /locus_tag="IV353_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9009966.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5138..6079
                     /locus_tag="IV353_001722"
     CDS             5138..6079
                     /locus_tag="IV353_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9009967.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6148..6879
                     /locus_tag="IV353_001723"
     CDS             6148..6879
                     /locus_tag="IV353_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9009968.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7023..9203
                     /locus_tag="IV353_001724"
     CDS             7023..9203
                     /locus_tag="IV353_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9009969.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9223..9705
                     /locus_tag="IV353_001725"
     CDS             9223..9705
                     /locus_tag="IV353_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9009970.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9721..10482
                     /locus_tag="IV353_001726"
     CDS             9721..10482
                     /locus_tag="IV353_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9009971.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10613..11947
                     /locus_tag="IV353_001727"
     CDS             10613..11947
                     /locus_tag="IV353_001727"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9009972.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12254..12739
                     /locus_tag="IV353_001728"
     CDS             12254..12739
                     /locus_tag="IV353_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9009973.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12732..13415
                     /locus_tag="IV353_001729"
     CDS             12732..13415
                     /locus_tag="IV353_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9009974.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13541..16186
                     /gene="polA"
                     /locus_tag="IV353_001730"
     CDS             13541..16186
                     /gene="polA"
                     /locus_tag="IV353_001730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9009975.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16231..17067
                     /gene="mutM"
                     /locus_tag="IV353_001731"
     CDS             16231..17067
                     /gene="mutM"
                     /locus_tag="IV353_001731"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9009976.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17031..17660
                     /locus_tag="IV353_001732"
     CDS             17031..17660
                     /locus_tag="IV353_001732"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9009977.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17712..18044
                     /locus_tag="IV353_001733"
     CDS             17712..18044
                     /locus_tag="IV353_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9009978.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18356..18847
                     /gene="nrdR"
                     /locus_tag="IV353_001734"
     CDS             18356..18847
                     /gene="nrdR"
                     /locus_tag="IV353_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9009979.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            18870..20243
                     /locus_tag="IV353_001735"
     CDS             18870..20243
                     /locus_tag="IV353_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9009980.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20243..21169
                     /gene="dnaI"
                     /locus_tag="IV353_001736"
     CDS             20243..21169
                     /gene="dnaI"
                     /locus_tag="IV353_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9009981.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21298..21738
                     /locus_tag="IV353_001737"
     CDS             21298..21738
                     /locus_tag="IV353_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9009982.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7392 a         3771 c         4774 g         5877 t
ORIGIN      
        1 gattgtgatt gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc
       61 caattaaggc tttttctttt tggatttttt tgtcataata aatattagat gatggaggag
      121 ataatatgat gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac
      181 ttatgcaggg aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc
      241 ttgtctttag tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg
      301 tctttttcgg atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg
      361 agaatcaacg ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa
      421 acaaaaagca agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc
      481 atcaaaagca gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg
      541 gtataaaatt cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca
      601 tggcgaaagc gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac
      661 agaaaaagga aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca
      721 attcgatgcc gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact
      781 agaagaaatg ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca
      841 ttatggggat cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca
      901 agtaaaaaca tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt
      961 tgaccgcaga tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat
     1021 gacagtgaat cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg
     1081 gcaagattta ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga
     1141 agatgttcct gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta
     1201 tacattagca gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc
     1261 taaaagtgag agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta
     1321 tatatgggac tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg
     1381 gcaagcacta aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa
     1441 aaagtattct actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga
     1501 attatttcac cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa
     1561 acaagtactg gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt
     1621 gacagaatca acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt
     1681 agtagggatc gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat
     1741 cgatactttt catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat
     1801 cgaagcaggg aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg
     1861 tgagatcccg gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat
     1921 cattaaaaag tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat
     1981 aaaattatat ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga
     2041 gattgccgaa ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa
     2101 cgatcgaaaa ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag
     2161 tgtttgatac atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga
     2221 aaattgaagg tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat
     2281 tcgatatcgt acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg
     2341 taaaattgaa ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga
     2401 ttaaagctat cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc
     2461 gataaaaatc gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga
     2521 agaatcattc tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag
     2581 gagaaaaatg acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata
     2641 tgtgttctat cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt
     2701 aggagttggt atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt
     2761 cgttactcaa tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca
     2821 aatgctagtt atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt
     2881 ttctgaaaag ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc
     2941 tattgcagca ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt
     3001 cacacaagga gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt
     3061 tgaaaactta tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga
     3121 tttagcaggt agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg
     3181 aatcgcttat ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt
     3241 agtcgacagt ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc
     3301 ttatggagta tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt
     3361 caatttaggt aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca
     3421 ctcactgatt ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc
     3481 ggtattgagc tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga
     3541 gactcagaca aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg
     3601 tttgtctatt ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt
     3661 cgcaccagca gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat
     3721 tgtaacaatc agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact
     3781 gatcgtttta ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga
     3841 accattaatc gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat
     3901 cgtgacaagt gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt
     3961 gatcgatgtg aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat
     4021 aagaagaagt ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga
     4081 gaagctactt ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta
     4141 ataagaacag ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg
     4201 cagatgcaag cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg
     4261 agtcacaaag agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg
     4321 accgaggagt tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga
     4381 cctctttttt tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca
     4441 aataacaaaa acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt
     4501 ctgttgagag ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat
     4561 gttagaacag atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt
     4621 atgtggacga tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag
     4681 atcgtcaaga aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct
     4741 catttctgtt atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg
     4801 ccccatcttt gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc
     4861 aatcagtcac attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg
     4921 atcccgatgg caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt
     4981 ctgtgcgaaa caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa
     5041 aactttccat gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta
     5101 tactgtttag gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta
     5161 ttacaattat acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg
     5221 caaatctttg gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt
     5281 gtctggaacc gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt
     5341 tgctgatacg atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt
     5401 aagataccgt ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt
     5461 gacaggtgcc tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt
     5521 ggtgattgat gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg
     5581 cccagatata cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt
     5641 tatggaaaaa cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg
     5701 ttggcttgtc cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta
     5761 tggtaaggat cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga
     5821 acggatcgct gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga
     5881 cggaacacca tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct
     5941 tgcttccatt gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca
     6001 tccgcgcaat cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt
     6061 tacagcttta attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct
     6121 atactagcgt tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc
     6181 catggcaaac atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag
     6241 cttgttttat taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta
     6301 ggaaagcaat tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat
     6361 tttttgcaat ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga
     6421 aaagaaacgt ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa
     6481 atctcgatga tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc
     6541 ttgtattatg aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa
     6601 gactggcgta agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg
     6661 aaaaacaata caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga
     6721 gatatgcaga caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct
     6781 gtattcggag ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt
     6841 gaatatcaga acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag
     6901 ctgtgacttt gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt
     6961 aacccaggat aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt
     7021 taatgacgga aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact
     7081 atcgtcctaa gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt
     7141 ttatcgcgcg ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa
     7201 tcgaagagcg gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc
     7261 taatcgatga acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga
     7321 aaatgcaaca agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa
     7381 cgattgctaa agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg
     7441 gagaggtgca atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg
     7501 aagaagcgtt gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat
     7561 ttcggacttg gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg
     7621 atgagcaagc agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc
     7681 ataaaatggt ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga
     7741 aagtattctt tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca
     7801 aaaatcctgc ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac
     7861 gctttattca gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg
     7921 agcaagcaat cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa
     7981 aaggtaaagt tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg
     8041 tagatgcgac agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc
     8101 aagcaaaaag agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag
     8161 atatggtagc tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc
     8221 agttgaaatc cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg
     8281 tttactccgc tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa
     8341 gtgctgtttc tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc
     8401 ctaaagctgt cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac
     8461 aattggattt tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa
     8521 gcccgcagct cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca
     8581 tttatcgtga agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct
     8641 taggaccaaa agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca
     8701 ttttggacaa cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt
     8761 cagtccaact ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat
     8821 tatccgtaga gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt
     8881 tagctgggct gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac
     8941 gtacagatgt attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc
     9001 gtaatgtcat tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc
     9061 atatatcgaa actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag
     9121 atatcgtaac agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga
     9181 tgctttcccc ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg
     9241 cagcagttag tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca
     9301 atgttcaatc cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta
     9361 gattttaatg cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa
     9421 catgaacttt gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg
     9481 gatttcaagt atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag
     9541 aaacagataa agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag
     9601 agaagaatcg atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt
     9661 gagacaaaaa gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct
     9721 ttggaaaaaa gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc
     9781 gcacctgaag agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac
     9841 tgtcttccta tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa
     9901 ttgtttttgg tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag
     9961 caaggatttc attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa
    10021 gaagtctatg atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga
    10081 gagaacgcca ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc
    10141 ttattgatga atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg
    10201 caaaatgaac aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt
    10261 tctgaagaga tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc
    10321 catgcgctag tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg
    10381 aaaggaaaag attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc
    10441 agaattaaag agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat
    10501 cctcctttcc aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt
    10561 gttgaatgta atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa
    10621 tcaaaataaa ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct
    10681 tgttttacat gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac
    10741 acaaagagat ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa
    10801 accagggatg acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg
    10861 agcaaaagaa ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca
    10921 aaattatacg agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct
    10981 ctctcatgta ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca
    11041 tggtgatccg caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct
    11101 tgcctattca ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta
    11161 tacaagtatc gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc
    11221 tatttttgcg tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta
    11281 ttattatggt gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc
    11341 tggttctgct tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc
    11401 attcggtaaa cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa
    11461 actggatctg aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt
    11521 cagtgaaaag atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga
    11581 gatcaaagca acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt
    11641 tttccaaccc catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact
    11701 tgatttagct gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg
    11761 aaacgttaag attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga
    11821 aaataatgta tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg
    11881 agatgttcaa aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt
    11941 actgtaatac aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat
    12001 cttgccaagc cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg
    12061 gtaataagcc caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta
    12121 tttgatgcag gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg
    12181 gagcagaact tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg
    12241 gagggtttct atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat
    12301 cgaggaaggc gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta
    12361 tttgggattg acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac
    12421 agaatatcaa cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat
    12481 ttcaaagcat cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga
    12541 accggttttc cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat
    12601 gaaggatgtg gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga
    12661 tgctgtcgtt atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga
    12721 aggtgaattg aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa
    12781 aagtttatgg atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc
    12841 tttgggtatt tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg
    12901 gtctgtggat cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc
    12961 cgagcttagc aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg
    13021 ttactcttgc atactacgat attggaacag taacgcaagc tttcttgaca gctactttga
    13081 tgttcatggg gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa
    13141 gagcaggaca ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt
    13201 tgagaagtag cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga
    13261 ttacagctta tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac
    13321 atagtggaat tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct
    13381 tggcgtttct gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg
    13441 gctataccga attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt
    13501 gttagaatga ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt
    13561 actcgttgat gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga
    13621 acgtttcaaa aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt
    13681 tgaaaatgtt atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa
    13741 aacgactttt cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg
    13801 cgaattcaaa gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta
    13861 ttacgaattg cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga
    13921 caaagatcaa tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag
    13981 taaagaagta aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc
    14041 agaacatgta gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg
    14101 actagccgga gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc
    14161 tatcaagcta ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat
    14221 gaagcaaagc aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa
    14281 aaaattggcg accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta
    14341 tgaagggaaa aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt
    14401 cctttctaaa ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt
    14461 cgttcatgaa ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat
    14521 gggcgacaat tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat
    14581 ttatgtcacg aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga
    14641 tccgactcgg ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta
    14701 tactggaaaa acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac
    14761 gaatgaaaaa agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca
    14821 atcagatgaa gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact
    14881 cttttttgct catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag
    14941 ccaagaactg gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc
    15001 tatcattttg gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga
    15061 aatgaagggc gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc
    15121 cggcgaagaa ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat
    15181 gggattaccc gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga
    15241 acagcttcgt gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa
    15301 aattcaatct acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca
    15361 tacccgttat gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt
    15421 gcagaacatc ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag
    15481 agaaaaagac tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc
    15541 gcatatctca aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc
    15601 cagcacagcg atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg
    15661 taggcaggca aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc
    15721 acaaaatcta ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa
    15781 atacccaggt gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta
    15841 cgtcgaaacg ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa
    15901 tatccgttct tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga
    15961 tattttgaaa atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc
    16021 tacgatgctg ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga
    16081 atcattagat aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact
    16141 gatcactgac agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac
    16201 agagcaatat aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt
    16261 cgtaaaggat tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg
    16321 cccaagatca tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc
    16381 atccaatcca ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg
    16441 atctcacact tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat
    16501 aaacatacgc atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta
    16561 cgaaaatttg gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc
    16621 atgaaattgg gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga
    16681 ctgaaaaagt cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt
    16741 ctaggtaata tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg
    16801 gcgaacacac tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg
    16861 gatcgtgcaa tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa
    16921 tcaggcggat ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt
    16981 tgtggaacgc cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat
    17041 tgtcaaaggc taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta
    17101 ccggtaaaag tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg
    17161 atgtgattgc cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag
    17221 tgtttggcca agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga
    17281 ttgtttttcc atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc
    17341 gaaaagcgat aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag
    17401 atatcccatt gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt
    17461 atactacgcc agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac
    17521 aagcgcagca gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata
    17581 ttttgttcga caacaatgga acaaaagaaa aacttgtggg acaaatcgaa aattggcttt
    17641 ttgctaatcg ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt
    17701 gagggatagc gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag
    17761 aaaaaatcgg cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag
    17821 aaacagcaga agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc
    17881 aggcagaaga agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg
    17941 atcgtagtaa aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg
    18001 aacagcgaat gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga
    18061 gatgtgactt gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga
    18121 ctaaatcagc ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt
    18181 gaaatttagt aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata
    18241 tccgtgtttt ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta
    18301 gttgtaaaga aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag
    18361 atgtcccaga tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg
    18421 acgtgcgatc cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga
    18481 acgaattgaa gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa
    18541 tcgcgataaa attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga
    18601 acaaatggaa caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga
    18661 agtatcgacg aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat
    18721 cgcttatatt cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa
    18781 agagttgcaa gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa
    18841 caattaagaa acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa
    18901 aaaatattta ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc
    18961 tgtatcttta tcagccacta atcggcggag aagccttgag tatctatttc accctgttat
    19021 cagaaatctc tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta
    19081 gtctaggctg cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct
    19141 tactggatgt ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt
    19201 tagaaccact cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg
    19261 aaaaagttgg tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata
    19321 cagcagaggg atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag
    19381 cagtgtacgc tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg
    19441 aatcagtagc attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc
    19501 tggcaaaaaa acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc
    19561 atcagcttta cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact
    19621 ttagtgaaca aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac
    19681 gtccggtgaa gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt
    19741 caaacgaaca tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac
    19801 cgatgaaata tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa
    19861 actggttact gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt
    19921 tgctgaatta tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata
    19981 cagtggcaaa tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca
    20041 ttcggcaaat aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa
    20101 ctggcaagcg aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg
    20161 aaaaacagat cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg
    20221 caaaaaagga gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag
    20281 caaacgcaat tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga
    20341 tgttcaagct tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag
    20401 ttacgctaaa ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac
    20461 gatgatcgct cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac
    20521 ttatgtacca acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt
    20581 aaaagcaatg gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac
    20641 agatggacga ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga
    20701 tccaaaaggg tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata
    20761 tttattagga gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca
    20821 ttttccgaca tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa
    20881 gttagatgca gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat
    20941 gagtagttgg atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca
    21001 gttaccaacc ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt
    21061 cactcaacgt ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta
    21121 tttgacaaaa gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa
    21181 gtccaactga catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa
    21241 atgaccggag acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg
    21301 agggctattt tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt
    21361 agcgcagata gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca
    21421 agcagtacga aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa
    21481 gtagaagcag aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat
    21541 ccagcgaata tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa
    21601 aaaataagcg tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt
    21661 gagatgccga tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat
    21721 gtttcattga acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag
    21781 aacgaaaagg tatatttttt aaggagatat ggga
//
LOCUS       DACDLI010000045        20569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDLI010000045 DACDLI010000000
VERSION     DACDLI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.045"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            236..580
                     /locus_tag="IV353_001738"
     CDS             236..580
                     /locus_tag="IV353_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9009983.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            585..866
                     /locus_tag="IV353_001739"
     CDS             585..866
                     /locus_tag="IV353_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009984.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            969..1283
                     /locus_tag="IV353_001740"
     CDS             969..1283
                     /locus_tag="IV353_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9009985.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1261..2955
                     /locus_tag="IV353_001741"
     CDS             1261..2955
                     /locus_tag="IV353_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9009986.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2975..4153
                     /locus_tag="IV353_001742"
     CDS             2975..4153
                     /locus_tag="IV353_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9009987.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4116..4802
                     /locus_tag="IV353_001743"
     CDS             4116..4802
                     /locus_tag="IV353_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9009988.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            4802..5962
                     /locus_tag="IV353_001744"
     CDS             4802..5962
                     /locus_tag="IV353_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9009989.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            5972..6847
                     /locus_tag="IV353_001745"
     CDS             5972..6847
                     /locus_tag="IV353_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009990.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            6844..7155
                     /locus_tag="IV353_001746"
     CDS             6844..7155
                     /locus_tag="IV353_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009991.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7145..7498
                     /locus_tag="IV353_001747"
     CDS             7145..7498
                     /locus_tag="IV353_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9009992.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7488..7889
                     /locus_tag="IV353_001748"
     CDS             7488..7889
                     /locus_tag="IV353_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009993.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            7882..8286
                     /locus_tag="IV353_001749"
     CDS             7882..8286
                     /locus_tag="IV353_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009994.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8298..8906
                     /locus_tag="IV353_001750"
     CDS             8298..8906
                     /locus_tag="IV353_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9009995.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            8926..9288
                     /locus_tag="IV353_001751"
     CDS             8926..9288
                     /locus_tag="IV353_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009996.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9291..9473
                     /locus_tag="IV353_001752"
     CDS             9291..9473
                     /locus_tag="IV353_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9009997.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9490..12810
                     /locus_tag="IV353_001753"
     CDS             9490..12810
                     /locus_tag="IV353_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9009998.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALDMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEKFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFKGIWSKF
                     TDWLGGTWESIKEGASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPHFKLSGKFNPLKGKIPTLGVDWYAKGSVFNSPNIIGIGEAG
                     PEAALPLKRSVLQEIGDRILSSTSVSSRAQTVQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            12861..13598
                     /locus_tag="IV353_001754"
     CDS             12861..13598
                     /locus_tag="IV353_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9009999.1"
                     /translation="MTDCIHSIVDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFTCTGLSKIQIYRNGLR"
     gene            13608..15899
                     /locus_tag="IV353_001755"
     CDS             13608..15899
                     /locus_tag="IV353_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010000.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNVGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            15923..18142
                     /locus_tag="IV353_001756"
     CDS             15923..18142
                     /locus_tag="IV353_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010001.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAETEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGA
                     DYSPVDGKLILIRYRYVDSGTGKIVLGLPINSNSITQEWKEFSITGTTPTFSPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGSTATPFQPNLLAEPYNMCREYPNENIADPKVKF
                     PIESGDHQIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLT
                     DVWSLTFTPTNVVAMPKLFRVYQYPRSTVGACQIDWLKIEKGNTRTPNISEYKYRGTG
                     MRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSKINSQLTASINEV
                     DTTAKGAQTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTG
                     YTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDN
                     LVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVANGILQFTSCRTLVLTRLSD"
     gene            18220..18462
                     /locus_tag="IV353_001757"
     CDS             18220..18462
                     /locus_tag="IV353_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9010002.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            18478..18675
                     /locus_tag="IV353_001758"
     CDS             18478..18675
                     /locus_tag="IV353_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9010003.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            18686..19705
                     /locus_tag="IV353_001759"
     CDS             18686..19705
                     /locus_tag="IV353_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9010004.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGLNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            19895..19968
                     /locus_tag="IV353_001760"
     tRNA            19895..19968
                     /locus_tag="IV353_001760"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19929..19931,aa:Ile,seq:cat)
BASE COUNT         7237 a         3106 c         4314 g         5912 t
ORIGIN      
        1 cgttgagtgg tctttttatt ttgcaaaaag gaggtaacaa caatgtatag accacaatac
       61 ttagaacaga agcatgaagt aatcactgtg caaaacggta acggtgagat agtacgaaag
      121 tatagaagac caataaagag caatacatat aaacgaaagg aaagcaatga agttattcca
      181 ttgtatggca aaagaatagc taagtattaa ataagattgc gaaaggagat gggacatgac
      241 cgaggaattc tatagatggt tattacagtt gataagagaa gatcgtttgg ttaagttcta
      301 tcagtctcct aaatggcgca ggcttagaga gaaagcgatg aaacgagatc actatgaatg
      361 ccaagagtgt agaagactag gtaagtatca tagagtagag aacgttcatc atataaagga
      421 agtcaaggat agacctgact tagctttaga tttagataat cttatttgtt tatgtgttga
      481 acatcataat gaagttcatg gcagatatct tacagcatta gataaacaag agaagaagat
      541 agaaagcttt gctaacttcg atgcaagtga aaggtggtaa gtgcatgatc atcaatgaca
      601 atggcagaga gtatgacaca gaaaagattg aagagtattc atcttatact caaggattaa
      661 ttaaacgttt gatatacgtt cgctatgtag gtattaggga tctgttatca gataactgtt
      721 gcagtaaata caaagtgaat caagtaagag aagcgttgaa taaagataat aacgttgaaa
      781 gaataaaaaa tatttttgga tatagcattg aagagattaa ttattacatt gacttcgctg
      841 aagctttcat tccgatggtg agataacccc cccttaaaat aaatcgcaaa ttttttgggg
      901 gtgatgaaac ggagggggct gtcaggaaaa gagatttttt cgaactttat catgaaagga
      961 gggctaaaat gtttaaaaac gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct
     1021 ctttaataag ccaattggaa agtcagaaaa caaatattga gccattctta gataatgttg
     1081 atcgttatat cagtttatgg gaaacggcga tatcactgga agaagatata tccgagaacg
     1141 gcattagact ggagaatggt aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca
     1201 aacaaatggg attgatgttg gataaacttg ccattactcc tgaattggta ggtgaagcaa
     1261 atgaatcaat tcctgagtta taagcatatt gaaaattggt tcaaagctat agaagaaggc
     1321 actatcaagg tatgcaaaga gcaattattg ctaaaaaatt atctagaaga aagagtcttt
     1381 actagagaag atatttactt cgataagcag atggtagagg attcaatcaa tataccagca
     1441 caatactttc cattcgaatt aattccgtgg gaaaaatttc tacaatgttt tatttatggt
     1501 gttcgatgga aaaaagataa aacactagtg ttcaatagat atctttcatt aatgggacgt
     1561 ggtaatggta aaactggttt tgcttcttgg aacaacttct ttctactaac cgctaaacac
     1621 ggtattaaaa attatgatat tgatatctat gccaataatg aaagccaagc aaagactagt
     1681 tttgatgatg tatttaaagt aattaaagat catcctgatt tagataaaaa agtatttaaa
     1741 gctacgaagg aagttattca aaatatcgct acaaacagca aacttcgtta taacacggca
     1801 aatgctagaa caaaagatgg gaagcgacca ggtgcaaacc gctttgatga aattcacgaa
     1861 aatgaagatt attcaatgat aaatgtggct acttctggtg gtggtaaaat tcgagattat
     1921 agagaatttt atgatacaac taatggtcat gttcgtggtg gtccgcttga tgacattata
     1981 gaagaatcaa aaatgattct ttctggagaa cttggaattg acaaggatgg agcagaattt
     2041 tctagtttgt ttccatttat ttgtcgcttg gataacgata atgaagttga tgatcccgac
     2101 atgtgggaaa aagcttgtcc aactattaat tacaatgcag atttaaaacg gaaaatgttt
     2161 caagaatact ctcaaatgca acgtaatgct ggtttaagac ttacgttcat gaccaaacga
     2221 atgaacagac ctatggaaga tacacgattt gctgttgctt catatgatga tgttctgcat
     2281 acgaaagaaa aagaatttcc tgaaaaaatg gatgaagtga taggaacagt cgattttgct
     2341 gatagacgag attttgccag cgttgggttg ctaggaaaat acgataaaga tgtttatttt
     2401 acacaacata cttttatcca cgaatcagcc cttcgattac aaaacatcaa acgagaggtt
     2461 atagatattt ctatagatca aggaaaatca cagatcgttc atggaaaaaa tatagaagct
     2521 gattatattg taggttggtt tcttgaaatg agtaataaat attatattaa aaaaatcgct
     2581 atggatatgt accgtgcaaa aatattgaag cccgctttag aagaagcagg ttttactgtg
     2641 gaaattgttc gaagcggatc tgttacacat ggtatgttaa aagatctggt tgatgacctt
     2701 tttattaatc aacgtttatt ttttggtgac gatgcgatta tgcgttggta ttgcatgaat
     2761 gtatatgaag agcatatttc taatggaaat atacgctatg aaaaaataga acctgaaact
     2821 agaaaaacgg atggcttttt ttcattcctt catggtttga attttttaga tgatatttat
     2881 gattctgctc ctgtaacagt cacaaatagc tcagtagaaa atacaggaac tggatttact
     2941 cctctagtat tctaacttga aaggaggtga gaaagtgggg atttttcaaa aggcggtagg
     3001 atacttcaca aaaaaagcaa cggttccttt agaagaatac ttttgtaaat tgcaagttga
     3061 ttttgtgtat cgaaaatttg cgattgaaac ttgtattgat ttgattgcaa atgcgatgag
     3121 taaagcggaa ttcaagtcat atgaagatgg aaaaaataaa aagaatgatc tttactatag
     3181 gctgaatgta gctcctaata agaaaaataa tgcaacagaa tttagaaaaa aactgatcag
     3241 gagattaata ttctacaatg aagtattgat cgtttctccg tctaataatt ctagcgaaat
     3301 atttattgcg gatagttggg atgtcacaga atatgcattg aaagatgatg tgttttctca
     3361 agtgcaaatt aacaacatag tccttgatag agaatttcta gaaagtgatg ttatctatat
     3421 aaaatacgca gatcaacaaa ttaggcaact agtcgatgcg tattatcaag cgtatgggaa
     3481 actcatttct agtgccatga atgtttacaa gcgctctaac gctcgtagat acgtactgaa
     3541 agggaattta ttccgaccgc aagacaatac aacacaagat caaatcaata aaatgatgac
     3601 atcacaattt aaggctttta tggaagctga taatgcaggt gcggtatttc aattacaaaa
     3661 tgagtacaca ttagaagatt tcagcggaaa cttccaaagc aattcaagag atataaaaaa
     3721 cttaatagac gacatctttg agatgacagc agcagcgttt cacgttccga aaaacctact
     3781 aaagggagac atgagtgggt tatcggatca agtggacgct tttttaatgt tcgaaatcat
     3841 accgattgct gaacttattc aggatgcgtt taacgctagt ctctatgaag tagaagaata
     3901 cttgtcaggg aattttgtac gtgtggatac aactatgatc aagattacta gcttcaaaga
     3961 tttggttgac gctattgatg taggcattag aaatggagta tttacaatca acgaaggaag
     4021 agaacgcgtt ggaaatgatc gctctgataa ggcgatggca gatgaaatat ttataactaa
     4081 aaacaatcaa caagtatcga aaggaggtga ggcgaatgac gacaatgaaa acatttctag
     4141 cagtaaagaa tgaaggcgca gtaccgcaaa tttttattca gggatttatt ggttctagtt
     4201 ggttctttga agggaatact gacaagggaa tcaaaaatat tttggatagt ctaggtgatc
     4261 aagaagaaat tgaagtagta attaattcaa acggtggaga cgtatttcaa gggattgcta
     4321 ttgggaactt acttaagtca aataaagcaa aagttaacgt tgtgattaac ggcttagccg
     4381 ctagtgctgc ttcaattatc gcaatggctg gcgatactat aaaaatctac aacaatgcac
     4441 aattgatgat tcaccgcgct tccacatacg gagaaggaaa tgtcgatgac ttccgtacga
     4501 ttgctgacca actggaatca attgataaat cggtaaaggc ttcatataaa acacgattca
     4561 atggcacaga tgaagcattg caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa
     4621 cagctttgag ttatggattg gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg
     4681 aagctaaaaa agaacaaagc gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag
     4741 aaaaaattgc tgcatttaca gcagcattaa ataaaacatt tggacaagga gatgtaaaat
     4801 aatgacagtt aaaaatttaa aaggtgtaac agctgcaagc gaccaattga tgaaagcttt
     4861 taaagatggt aacgaagaat cttttagcgc agctatggta agcttatcta aggaaattca
     4921 ggataaaatt ttagaagaag caacagcaaa aaatcaagat caattagtat taatgaaccg
     4981 tggtcagcgt gtattaacta cacaagaaac aaaattctat aacgaagtag tgaaaaacga
     5041 aggttttgca ggggtcgaag aattagtgcc agctactgta tttgaacgtg tatttgaaga
     5101 tttagaacaa tctcatccac tattgcaaaa aattactttt gttaacacaa ctggtgtaac
     5161 agaatggatt gtgtcacgtg gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt
     5221 taaaaaagtt ttagataatg gctttgatgt aattaacatg aagcagttca agctatcagg
     5281 ttatattcct gtatgtaagg caatgcttga tttaggtcca gtatggttag atcgttatgt
     5341 ccgtactgtt ttagtagaat cattgagaat tgcattagaa caagcaattg ttgatggtac
     5401 tggtaaagat atgccagtcg gaatgatgcg tgacatgagc aaacaaacta gcggagaata
     5461 tgctgaaaaa aaagcagaac ctattacagc tttagatgct gtaactatgg gcggtttgat
     5521 ggcgcgacta tcaaaattca atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa
     5581 tccttctgat gtggtcctaa ttgtgaatcc aactgattac tggtctaaag tattcccagc
     5641 taagactgta ctaactgcta atggagaata cgtacaagta ttgccagtac cagtttcaga
     5701 tttgcagtca acggctgtgc cagaaggaaa agcagttatt ggggtagcct cagattactt
     5761 catgggtgta ggatctacac taaaaattga agcttcagat gaataccatt ttgttgaaga
     5821 cgaacgcatt tatctagcta aacaatatgc aaacggtcaa cctaaacgta acgatagttt
     5881 cattgtatta gatattagcg ctttgggaac tactactaca actacaaaac caacaaccac
     5941 aacaactaca acacaagcgt aggtgatcag aatgaagtat attctttgtc agccggcaat
     6001 caatcggttt aaatgggagc ttgaagtttg tttaactaat ctgaagaaac taggaatcaa
     6061 agatatcgta ttgcttttca gcagacacga tgatcagatt cctatttttt ttgagaagga
     6121 atatggtgtt gaagttcatg tgtacgatga tctgcgggac gacaaagagt atattccttc
     6181 gattaaacca tatttatggt ggaaatattt agaagaagat cattcgcgtg aggacgaccg
     6241 atatttctat atcgattcgg atgtcatttt caataaaaga attaatttgc gcaaattgcc
     6301 ttctaaagat gatgtttggt attgtagcga ctgctgtagt tatctaagtc ttgattatat
     6361 tagaagctgt gaaaacggag aaaatattct aaaagatatg gcaaacattg taaatgttac
     6421 agtagaatct ttggaaacta taaacactaa ttcaggaggc gcacagtggg ttattaaccg
     6481 tcctaaagcg aattattgga aaaaggttta tctggattct aatcggctat atcgctacct
     6541 tagagggcaa aaaacaaata tacaaatctg gacagccgag atgtgggcac agctttggaa
     6601 catgatgtat ttcaatattg gtcctaaagt tcacgaggaa ttagactttt gttttgctac
     6661 tgatccaata gaaaaagtta aagaagtaaa aatcttacac aatgctggag taacaacaaa
     6721 cgatgaagat ttatttttca aagggagata cgtgacttct acgccttttg atgaagattt
     6781 atcatttgta aacaagaaaa aatgctctta cgcatatgtt aaagcaatta aggcggtggt
     6841 tagatgacgc ctgaacaagt gactgaagaa ttgctaatag ctgtgaagga taatatttac
     6901 gttacctgga acgaagaaga tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat
     6961 cttcaaagta aagtgagtac aacactttct ttttctcctg aaagcttaga atacggattg
     7021 ctaatcgaaa gatgtagata cgactggaat cgtgctttag atgagtttga acaaaatttc
     7081 gctagtgagt tattaggttt cattcaacat tatgcgctac aagaatatat tgcaggtgat
     7141 gtgaatggcg aataatcgta gactcgaaga aacattcaac gatggttggt taaagatttt
     7201 gacgcaaact accaaaagaa atgaactagg aaaaaagatt ggtgtagaag atacagaaat
     7261 cacttcttta aaatttagaa atctttccat gagagatagt gatataacag ctatggatgc
     7321 gatgggatcg aaattaacta agaaagtaaa gactccattt catccaatcg ccaagaaatt
     7381 taataaagat caatatttta tcgtaatcga tagtatgcgt tacaacgtta tctatgccga
     7441 ttacgataat ttttatatct atttttatct tgaaagtgtg ggtgaatatg gtgattgata
     7501 attctaaaga aaaagaacgt ttaaataagc aaatttctgc tatcaaaact tccttagaag
     7561 agcattttgg cctcaaactc tttcaagact ccgtaggcga ggatgagcta cctgatgatt
     7621 ttaattactt cattctcgaa acaggagaaa tagaaatgat cactgagcca aaatatagcg
     7681 tgggtcaaaa tctatatcta actttctatt cagaaaatag agaagattta acaggagact
     7741 cactagatat tatttcattg attcaaaatc gttcgattcg ttttcagaga atggatccca
     7801 atcatttaaa actagagaac caagatcgct atatcgatca attggtattt acgtttagac
     7861 gattattgaa gagtgattgt catggctaaa aatagttggg agctaaaaat aaatggacat
     7921 gatgaacttc ttgtgcggat ggaacgctat tcaagcgaga gcgaacgact gattaacgaa
     7981 gcattgaaat caaaaggttc ggctattgca gtggatagga ttacagaaaa aattcctgtt
     8041 tctgaagcag atttaagaag agggcaccaa cacgcaaaaa atagtcgtcc acttaagact
     8101 caatacatta atttgggttt catcattaga cctacaagaa aatttgagta tttaaaatat
     8161 cctgatttgg ggataggtac ttctaaaaga aatcagccgg acgaatttat gagaagagga
     8221 ttaggtcttg cacttgatcc aattacagaa cttctgattc gtcaattcga taaattaaat
     8281 aaataggggg aacaacaatg gctaaaacaa aaactgtagt aacaacgttc gataacgtaa
     8341 gtatcaaacg aattgctttt aattttaaga acgcaggaaa tgcaatcgca acgaattgta
     8401 atggacaatt agatggcgaa acagaaatgc aaacggtggt taaaaaatgt ggagcgacag
     8461 aagtaaaatc aaaatctaaa ccaatcaata tgacggtaac aattactgca catgtaccga
     8521 tggaagttta tcgacgtttc aatgggttga aacaagatgg acgtattaaa ccaggcattt
     8581 actcttacgg tcctgattcc gtaggcgaag atttctcact tgctgcagag atcgtggatg
     8641 acttcgaaga aaatagaaag ttagttggta tgttagcatg cacttcgaat acaggattaa
     8701 cattctctat tgaaaatggt gcggatgaag tagctgcgtt agaactagaa acaaaagtta
     8761 tgcaagatga atttggtaaa ttttatcatg aagcaattgt tgcagaactt gaagaagact
     8821 taacagatca atggatgacg aatctatctg ctgatgtgat taaaaaaact tcagttgtga
     8881 caactacggc cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac
     8941 tcaaaaattg aactaaacga tggaacaatt ttgaatttag aacctaaact gaatatcaag
     9001 aaattattga tgatcaatag agattttaac acagacgagt ttgcaaaaat gactgtggga
     9061 aaaggatcca tggatatttc tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc
     9121 caagcgaaca tgactgatta tatttcattc gatgaattta tcgataaatg ggattttgat
     9181 atggctactg ccagctatat ttatcaattg atgatgttca aacaagcacg cgatgcttat
     9241 caaaaagaat ttgaaaaagc aaataaggaa aaaaagcttc aaaagtaaaa attccaaagc
     9301 tcttagttga aacgtgggtc gatgtctatt cgatgttgac cgacgttttt tctatgcctt
     9361 cagatttggt tttaagcgat atctgtttag atgacatttt gcaaatggct tacaacaaga
     9421 gtgcttatga aggatggaaa aactatgcaa taaaccaatc ccagaaaaac taaagaaagg
     9481 aggtaaaaaa tggctaaaaa gagaacagaa gcagaagtaa catttatagc taacgatgac
     9541 ggattgaaat ctacgttaaa agaaatcagc gctgaattaa ctaaaaatag agcagaatta
     9601 aaactagaac aagctcaatt acaacagact ggttctgaat cagacaagtt aggaagtaaa
     9661 ttatcttctt tagagaagca gtatgaatta caaagtcaaa aagttgaagt aactagtcaa
     9721 cgtttagcca atgcgaaaaa atattatgga gaaaattcca ccgaagttca gaaacttgag
     9781 agagaactga ttaatcaaca aacagcgcaa caaaggttat ctaatgatat cgataaaacg
     9841 agtaaagcat tggctcaatc taaaggtgaa tttaaaacct atgagtcaac tatgaaggat
     9901 ttagataatg agcaacaaca cctaaaatca agtgcatcat tagtagaatc cgaatacaaa
     9961 aaatggcaag caactgctgg tcagtctgct accgaatctg aaaaattagc aaaagcacaa
    10021 gaatatgtcg gcaaacagag tgatattgct gaacaaaaaa tcgaagtgct aaaaaaacaa
    10081 ttagatgcca cacaaaaaga atttggagaa acatctaccg aagctttaga tatgaaaact
    10141 aagcttaatg atgcggaaag agaattcgaa gaattaagta atgctgctaa aaacgttgat
    10201 acatccacag tagacgatat cggcaaaaaa cttgatatgg gcaatttaat ggaagctact
    10261 gatcacttgt cagtgatcgg agataagctg attgatgtag gcagtaagtc tattgaagca
    10321 gctggaaaag cacaagctat gcaggcccaa tttaaacaag tctttggctc tttagaaggg
    10381 gaagcgcagg acgccgttga gggaatggct gaaaaatttg gaatgttacc aaatacgatc
    10441 aagcctgttt ttacgcaata tacgtcaatg tttaaaggac ttggatatga tacaaaagaa
    10501 gctatggagt tagctggtga cagtactcag ttagcagcag atgcagcagc tttttacgat
    10561 aagtctatgg atgatgctag tgaatctctt aattcattca taaaagggaa ctacgaaggt
    10621 ggagaacaaa taggtctatt tgctaatgat actcaaatgg ctgcttatgc tgttaagcat
    10681 aacttgatac cagcgactga aggagcaaaa aaagccagtg aagagtcatt gttagccgtt
    10741 gaaaaagcac aatctaagta tgctgatgct attaagaaac atggcgaagg atctttagaa
    10801 gcaagagagg ctgctttaaa acttaaagat gcgcaagata aaataaatga agaattaggc
    10861 ccacaaactc aaaaatggtc tgatttagat gaggctacca aacaagcagt tcgagttcaa
    10921 tatgctgaag atatgcaaaa attagccggt gctacaggtc aggctagtag agagtctgat
    10981 ggtttagaaa accaaatgac aagagcaaag caagcattag aagattttta tgcctcgttg
    11041 ggtgaagata tattgcctgt gtttatcaaa gggttacaag caggcgcgaa agctttgcaa
    11101 ggattagccg aatggtggag taaacttgat ggaccaatga aaaatttcat tttagctctt
    11161 ggaggaattc tagcattatt aagcacatta gctcctgtta taaccgcagt tgttacgata
    11221 gttggcacat ttggttctac agttttgttg ccaataatag gaattatagc gggcgttgca
    11281 gctgtaatag cgattgtaat cacagcgttt cagaactggg gtgcaattac tgactggttt
    11341 aagggaatat ggagtaaatt cactgattgg ttgggtggta cttgggaaag cataaaagaa
    11401 ggtgcctcat cagtttggga tggagttaaa gaaacctggt ctggatttgt agattgggtt
    11461 caagaaattt ggcaaggagt ttctgattgg tttggagagt tatggagcgg attagttgaa
    11521 ggagcttcca acatctggca aggagtccaa gagacttggc aagcattcgt tgattgggtt
    11581 tcaaatattt ggaacggagt caaagaagta tggtcgatta tttgggcaga cattgtagga
    11641 attgttcaaa taccatggac attaataacg tcattgattc aagctggtat taatattatc
    11701 gtgggtatct ttgatgtagc tggacagtta ttaggcgcag cttggcaagc tgtttggaca
    11761 cctatttctg atttccttaa aaatacttgg gatactatga cacaatggat aagcatcgct
    11821 tggaatggaa ttgtaactac attccatact atatttgatc cagtagtggc atggtggaat
    11881 ggtatatgga catctattag tactacggct tcaaatattt ggaactcaat tagtgcaaca
    11941 gcttctagta tttggaatag tatcaagaat acaatcacta gcttggtaca agcagctgct
    12001 acagtaattc aaaatatttg gtcaactgta tctagttggt taggtggaat ttggaattca
    12061 atcagctcta cagcatcaaa tatctggagt agcgtgacta gtagtataag caatgctata
    12121 aacgcagcta aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg gatcagcgga
    12181 atttggaacg gtatcaaaaa cactgctttg aatctttgga atggaattac aagcactatt
    12241 agctctaaag taaacgatgg aaaaaatgca atttcaagcg gttggtccaa tctaacaggt
    12301 attgtttccg acatattcaa taatgttaaa agtacgattg ctaacatttg ggaaggtatt
    12361 aaaaagactg ttagcgctcc gattgattgg attagagata aaatcagtgg catctttgat
    12421 aatctgaata tttcgatacc acatattccg ttaccacatt ttaaattgag tgggaaattc
    12481 aatccattga agggaaaaat cccaacgttg ggtgttgatt ggtatgcgaa aggtagtgtg
    12541 tttaattctc cgaatattat cggtatcggt gaagcaggac ctgaagcagc tttacctttg
    12601 aaaagatctg tgctgcaaga aattggggat cgtatcttga gtagcacctc agtttcatct
    12661 agggcacaaa cggttcaacc tgtgaacaat tacgaattca atttcacaat tgatggtaac
    12721 gcagatgagg ttactatgaa gcagacaact caacaaatca ttgacagcat tacaaaagtt
    12781 caaaatgata atgcttcggc atggcgttaa acaggagagt atttctcctg ttttcttagt
    12841 attacaaagg atgtgaaaaa atgactgatt gtatacattc tatagtcgat ggatttcctg
    12901 attatttgca taaattggct ttagctgaaa gaccaaccat accttctcca aaaagacaga
    12961 gagttgaaac ttctgtttta ggacggctag gtggcttagt acaagattat tcgtttgaag
    13021 atatgtcgtt tactttgcac tataactatt tagaggatgt ggaagaccat caagcgttca
    13081 agcaatcgtt ttatatcatg cgtcattggt taaattatgc aaagaaatta gaattctctg
    13141 atgatcccaa cgtctattat gttatccaga ctatcgatat tggggatgca gaaaacgata
    13201 ttgttgaatg gggagagttc gatgtaaata tcactgcgaa accattcgca agagttcaag
    13261 aagatgtacc tataaccgta gataaaccac agtcatttaa cttgctgaat aatagtttag
    13321 aagaaagttt tccaaagatt atcatcactc cttcagctac ttcatgccag ttcatcttaa
    13381 atgattatgt gtttagtttt gaaggcttag tagtaggaac tgatgtagtc attgatagtg
    13441 atttaatgct ttgctacgaa gagcaatcgg acggagatat tttagatcgg tccaacaaaa
    13501 tgaagaccat gcaatatcca acgttgcaag tggatattaa tcattttact tgtactggtt
    13561 tgagcaaaat acaaatttat cgtaatgggt taaggtaggt gaaatagatg atcgataatt
    13621 tactaactat ttacgataaa aatgacgcga ataatttagc tgaacattta tatgatacgc
    13681 aaggtttagg cgctttgtca gactggttaa cagctactgt tagcaataaa ctaaacggag
    13741 ccgagatatt tcagggtact tattcaataa acggaactaa tgcagatttg attgtagaag
    13801 gacgtattat tcagtgttat gtagatgaaa atcgagcaaa acagcgtcta cggatttatt
    13861 atgcaaaaac ttctgtaata ggcaatacga tagaagtaaa agctgaacct attttcaatg
    13921 atataagaaa atcggtgttg aataaatatg acagcggaac agaaaaaatc actgctactc
    13981 aggcatggca aaacgcaaaa gttttagcga aaccagctat cccttcgcag ttttctttct
    14041 cgtcattagt agatacgctt gctaatgtga agatagaaaa ggcgaatttt ttagaattct
    14101 ttggtggaaa agagggatct attctagatc gatttcatgg tgagtttcta aaagataata
    14161 acacattacg tcatgaaaaa agtctaggta cggatcataa aatcaaagcg atttatacta
    14221 aaaacttaac tggtcttgac ttagagattg atgctcaaag tgttttagtt ggagtttatc
    14281 catttattag cagctcttca gaaggagaag acgagatcac tctaccagaa gaagttattt
    14341 tcacggatta cgtggatgat tatcctgctg gatatgtttc ttttgttgat tttaaagaca
    14401 aagcgactga tgtagccaca ttaagggaag ctgctaaaga ctggttgaaa acaaacatag
    14461 ataaacaaaa accacaagtg agtggttcga ttgaattagt accattgagg catcaaagag
    14521 gctatgaaaa atttgttgat ctagaaaaag tttcgatggg tgacggagta gatgtgtatc
    14581 atccacagtt aaaagtgaat atgtcagcga gaattgtgga atatacgttt aatgttctaa
    14641 ccaattcata cgataaatta gttgtaggaa acgtcaaaac aaacttctta gaaaacacag
    14701 agaataatgt cagcaatttg attaatgatg ccattgatca attgaaaaac gttggcgaaa
    14761 tcagtgattt aatcaatgat attgtagatc atcaaactga tatgattact ggtcaaaatg
    14821 gtggttatgt tttattagat cctaaagaag cgcctagtcg tattttgatt atggacacac
    14881 cagataagaa taccgcaagg aatgttttac aaatcaacaa tgctggtatt ggtttttcta
    14941 aaactggcat taatggaaca tatgaaacgg catggacgtt agatggcgga ttcaatgcct
    15001 cgtttattac ggctggtgaa atagtaggaa ttactattag aggtactaca ttaattagtg
    15061 atggtgctga ttatagaaca agtattgcta atggcaaaat gacttggtac tcaaaaaaag
    15121 ttaacaaaga tattatggag ctagaagcac gtgattatgt aagtgctgat gccggtattg
    15181 tatcatacac catgaaaact ggtggtggtt tcatgattag aaatccacag ggtaacttgg
    15241 tttttagtac gtgggataat ggtaataaca gaccgtttct atcttttggt gcgcccaatt
    15301 tcaggtatag caatgctagt tatgtaactt ctggcgacgg tagttcttta agcattaatg
    15361 gtagtgcggg taactcatgg gaatttaagg tagctggtag gactatgaaa tttactagtg
    15421 atggtatgct aacgttacca ggttgttttt ttggttcatg ggaagatggg aaacttgcta
    15481 ggtttgaaca atcaacggta caagtatata aagattttac tgttagaggt actaaaaact
    15541 caactgtacc aacagaacat tatggacaac gactattgaa cgcttatgaa actccagaat
    15601 attatttcgc tgattatggg gaagccgtta caggtgacga tggtaaagtt cgtgttgata
    15661 ttgaccccat gtttgctgag acagtaaatc taagtcggta tatgacacat gtgacaccta
    15721 cagaactagt tttgtgtgct gttactcatg aagatattga ccatttcatc attgaaacta
    15781 gtaagccaaa cgtattagtt agatggaatt tagtggcaca ccgtctaggg tatgaagata
    15841 ttagattaaa agaggataca gcatatgata gcacagtgct tgaccaaaaa cgtttttaaa
    15901 acgaagacaa ggaggtatat aaatggctag cagtttatat aatttggctt tagatttcag
    15961 caaagaatta aactacacca aagctattat ggctcgtcag ggtgataaag ggattacggt
    16021 gacggttaaa ccatatttaa atggcttgca gatggatacg agtggcggaa catttacttt
    16081 aaaaggaaca acaccatcta accgttacgt agatagtgtt gcaactagcg taactagtga
    16141 agaagtcacg ttttctcttg atggcacatt tatgagtgaa gcaggatatt ataaacactg
    16201 ctacgtagaa tatagaaaag acaatcaaat tctaacaacg caagatatca tttttttctc
    16261 actaggagtg tctgacattt cgcaaggtca agccgatgaa tatgtttcgc aattagaaga
    16321 gttgattcga aagtataatg aaacttttga tgcttttatg gctgaaacta aaggtagagt
    16381 ggatagctta aatcaacaga ttactgattt aactggtcaa gctaaaacgc tacaagacaa
    16441 gttagatgct ctgaaagaag aaatttctaa gttaggtaac ttacaagtga tgtacagtaa
    16501 cagcattgat ttcgggggct atgattattc ggggaatccg aatttgttaa gtaagctatc
    16561 atacgactta attgaaaatc aaaatacttc agctggaaca ctttctaaag gtgaaaactc
    16621 gtttaaatat aataagatat cagctgaaac ggaaggtgga gtagagttat attataaacg
    16681 aagaggtata gctaactggt taccctctaa taaaacgctt gtaatgaccg ttaaacttag
    16741 agctggagcg gactatagtc cagttgacgg aaaacttata ctgattagat ataggtatgt
    16801 tgacagtgga actggcaaga ttgttttagg cttacctatt aacagtaatt cgataactca
    16861 ggaatggaaa gagtttagta ttactggaac tactccaaca tttagcccac aagcatacca
    16921 tccttggata caatttaggg ctcaagatgg gatacttggg gaaatagaaa tgagctatga
    16981 catcaaaatc gaagaaggct caacagctac accattccaa cctaacttat tagcagaacc
    17041 ttacaacatg tgtcgcgaat atcctaacga aaatattgcc gatcctaaag ttaagttccc
    17101 aatcgaatct ggcgaccacc aaatatatca aggttacaca gaagaagagc ttatgatagg
    17161 tcaaacgtat actatcacgc ttaaaggaac aaaacccgca agtcaaacct ttgtagcgta
    17221 taatcattgg actgctcgtt taggagaact aaagccggtt gatgggttga cagacgtatg
    17281 gtctctaaca ttcacaccaa cgaatgttgt ggcgatgcct aaacttttcc gtgtttatca
    17341 gtatccacga tcaacagtag gcgcatgcca aattgactgg ctcaagatcg aaaaaggcaa
    17401 cacacgaact ccgaatatta gtgagtataa atatcgtggt actggtatgc gtgattcaaa
    17461 caatccaaaa gattatgttt gggatctagc accagaatat gtcgaagata atttggccac
    17521 agatattaaa atttctgaaa ttactggtaa agcaaacaat tataccgatg ggaaagtatc
    17581 gaagattaat tcgcagttga ctgcttcaat taatgaagta gacaccacag ctaagggtgc
    17641 tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa ttagacaata agatcgatga
    17701 acgcattaat gatacagcta ctaccgcatt ttatggcgca caaggtgaag tctctaacaa
    17761 aacgaaagta gcggagtatg gagtaggaac agggtatact actacagctg taggagaaac
    17821 acatttcgaa cgtcaatcta acggatacgt taaatgctta aaagctggta agtattcaat
    17881 tagtgcgcaa atacgtgtgc aattgggcgg gaaatatgct tcatggttat atactgattt
    17941 atataaagat ggaacttctg ttgataattt agtatcttat ggggtcgacg ccttgcaaaa
    18001 tagatttgca gctagcggaa acattgtaac tgatttagaa gtaaacgatg tactaaatat
    18061 gcgtactgaa atcggcgttg caaatggaat tttacaattt acatcttgcc gtactttagt
    18121 cttgactaga ctatctgatt aatacaatat ttataatttt gctatcaaca cgctcaaagg
    18181 agggtgtttt ttattttgca atgaaaggag gatagttggt tgaaagacga agcaatacaa
    18241 gacgtggtag aacgcttagt gcgtattgaa acgaaactgg ataattacga attattacgc
    18301 gaaaaagcgg aaagtgcaaa agatagagcg gatcaggcat attctattgc gcttaataat
    18361 gcggaagata tcaaagaaat gaaagccaat aataaatggt cgtggggtta catgatcggt
    18421 ttaggcatta cgatcattgg ctatttcttg actaaattgt aaaggaggtg agaagaaatg
    18481 attttacccg ataagtatta tcaagtcatt aaatggacgg ttttaacagt attaccagct
    18541 gcatctgctt tagtagccat gttaggcaaa gcctatggat ggaatggaac agatatgaca
    18601 gttctgacta tcaatgcagt agccacgttt ttaggcgtta tcactggtgt gtcggcttat
    18661 aatttgaaaa aataggagga aacaaatgaa aaagaaaatt actattactg cgatgagcct
    18721 attaacggct ctttttttat tgccaattaa tgggttcgcc tatacgatta acaatgaatt
    18781 taatttgggc ctaaacgaag gtagctcaca agtagcaaat aatcagtaca ttttactgca
    18841 tgaaacggct aatgaaacag caacaggacg caatgaagcg caatatatgc aacgttcatg
    18901 gactagtgct tatactgctt atattgtggg agacggtgga attgtttacc aagttggaca
    18961 acctggttat gtacagtacg gtgctggttc gtatgctaat gctaacagtc ctgtgcagat
    19021 tgagttacaa cacacacata ataaagcaac ttttgaaaag aactataaag catacgttga
    19081 attggcgaga gattccgcta agaaatacgg tattccgctt acattggaca ctccttataa
    19141 ccaaccagga atcaaatcac atttatgggt aacacaaaat atttggggcg atcatacaga
    19201 tccttacggt tatctttctg aaatgggcgt aagtaaagaa aaattagcat atgatttagc
    19261 tcatggattt accgatgaaa atccaacaac ttctgaaaac aagcctgtca ttgatccaac
    19321 acgagctggt gcagctaatc ctacgctgac agatggaaca aatcacgccc acattgatca
    19381 gtttggggaa atcgaaaacg caaacttgca tgtggctgga tggcacattg ctaactataa
    19441 atacgagtat attttcatta tggactacaa tactgggaaa gaattagctc gagtaagagc
    19501 tgatggaatt tatagaccag atgtaaacca agcttataat acttctggaa atgttggtta
    19561 tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa gtctatgtaa tgatgcgggc
    19621 aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg acaaacgttg
    19681 gtatttaaat attcctaaac gataaaaata gctcctcgtt gaggagcagt acataactat
    19741 attgacaact ataaaaatta ttcgataaaa tagtgatgtt atcgcatatc ttcactatca
    19801 cccataaata gtcacactcc aagctatgcg ataacaggtt tgttgccaca cattctactg
    19861 gttgattgtt tatggcttta tgtggcaaca accagtaccc ttagctcagt tggtcagagc
    19921 agacggctca taaccgtccg gtcgtaggtt cgagtcctac agggtacatt aacgtagcca
    19981 tttgaatcgt tctgtgttag aatttttttg aagagtatta tacaagctaa agcttttctt
    20041 cattgccact caaatgagtg gcttttttat gtatcctttt atggattaat gaaaggatgt
    20101 ttcacatagt tatacttctg tatatttgaa aagttttact ttgattttta aatagaaaga
    20161 catttgggtt aaattgtgag ataataataa agaagagttt aaagcgcacc ccaaaccact
    20221 tccccataag tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc
    20281 atatttttga aaaaaagtga gaaaaaaggc ttacaattgg agtggtagtt aattagtgac
    20341 ttatttttga ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc
    20401 ttaatactaa cttaaaaaat atctcctttc acaagtatgg tgataaaatt cgttccgggc
    20461 taccttttta ggtagcctac tttaatcttt atacctttct ggatcaacga aagtatactt
    20521 tatatagtca taacgccgat gatcgctacg tgcgtccggc acgtcagtc
//
LOCUS       DACDLI010000046        20252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDLI010000046 DACDLI010000000
VERSION     DACDLI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.046"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(50..1234)
                     /locus_tag="IV353_001761"
     CDS             complement(50..1234)
                     /locus_tag="IV353_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9010005.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1259..2266)
                     /locus_tag="IV353_001762"
     CDS             complement(1259..2266)
                     /locus_tag="IV353_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9010006.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2438..2797)
                     /locus_tag="IV353_001763"
     CDS             complement(2438..2797)
                     /locus_tag="IV353_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010007.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2769..3140)
                     /locus_tag="IV353_001764"
     CDS             complement(2769..3140)
                     /locus_tag="IV353_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9010008.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3157..3459)
                     /locus_tag="IV353_001765"
     CDS             complement(3157..3459)
                     /locus_tag="IV353_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010009.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3476..3946)
                     /locus_tag="IV353_001766"
     CDS             complement(3476..3946)
                     /locus_tag="IV353_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9010010.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3921..4199)
                     /locus_tag="IV353_001767"
     CDS             complement(3921..4199)
                     /locus_tag="IV353_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9010011.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4196..5245)
                     /locus_tag="IV353_001768"
     CDS             complement(4196..5245)
                     /locus_tag="IV353_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9010012.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5202..6131)
                     /gene="cpaF"
                     /locus_tag="IV353_001769"
     CDS             complement(5202..6131)
                     /gene="cpaF"
                     /locus_tag="IV353_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9010013.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6263..8017
                     /gene="ade"
                     /locus_tag="IV353_001770"
     CDS             6263..8017
                     /gene="ade"
                     /locus_tag="IV353_001770"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9010014.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8115..8777)
                     /gene="trmB"
                     /locus_tag="IV353_001771"
     CDS             complement(8115..8777)
                     /gene="trmB"
                     /locus_tag="IV353_001771"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9010015.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8847..9626)
                     /locus_tag="IV353_001772"
     CDS             complement(8847..9626)
                     /locus_tag="IV353_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9010016.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9833..11044)
                     /locus_tag="IV353_001773"
     CDS             complement(9833..11044)
                     /locus_tag="IV353_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9010017.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11037..11774)
                     /locus_tag="IV353_001774"
     CDS             complement(11037..11774)
                     /locus_tag="IV353_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9010018.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11912..12346
                     /locus_tag="IV353_001775"
     CDS             11912..12346
                     /locus_tag="IV353_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9010019.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12339..12683
                     /locus_tag="IV353_001776"
     CDS             12339..12683
                     /locus_tag="IV353_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9010020.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            12863..13873
                     /locus_tag="IV353_001777"
     CDS             12863..13873
                     /locus_tag="IV353_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9010021.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(13942..14886)
                     /locus_tag="IV353_001778"
     CDS             complement(13942..14886)
                     /locus_tag="IV353_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9010022.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(14870..17590)
                     /locus_tag="IV353_001779"
     CDS             complement(14870..17590)
                     /locus_tag="IV353_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9010023.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(17587..18807)
                     /locus_tag="IV353_001780"
     CDS             complement(17587..18807)
                     /locus_tag="IV353_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9010024.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(19088..19933)
                     /locus_tag="IV353_001781"
     CDS             complement(19088..19933)
                     /locus_tag="IV353_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9010025.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         6188 a         4073 c         3327 g         6664 t
ORIGIN      
        1 aattccctct tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca
       61 gacgctccac atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta
      121 cttttgattc ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat
      181 cgatttcaca accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat
      241 tctcgccgat accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac
      301 cgccgatgta ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt
      361 tttcatagtt cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac
      421 cagatttctt gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta
      481 aatatgggag aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta
      541 atggtgtgaa tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata
      601 caccatttcc taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt
      661 ttgccgcacg ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt
      721 tgaaatcttc ataataagac accggtaagc tatataaata attttgcttt ggcattgttg
      781 agtggaaaga tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg
      841 ctttgatccc cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa
      901 tgcggtccat tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa
      961 caacacggtg ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca
     1021 actgatctaa cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga
     1081 atttttgatt atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga
     1141 cgatcccttt cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg
     1201 aactcccagc attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt
     1261 acagagcaga tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt
     1321 cttttaatga agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt
     1381 ttttctgtaa gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca
     1441 attgaatcat tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt
     1501 gatccgtttc taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat
     1561 atttcattga ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg
     1621 ttaaaaactc ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg
     1681 caaaatcgac aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat
     1741 ttgcttgtgt caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca
     1801 caccaattcc ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca
     1861 agttgcccat tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata
     1921 gttgttcaat aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg
     1981 cctgcaaatg ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt
     2041 cttctggctc gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg
     2101 tctcttttgt cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg
     2161 cattttcgac ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag
     2221 cttccaggtt ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt
     2281 ctagatatat tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt
     2341 caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg
     2401 ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat
     2461 cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata
     2521 gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg
     2581 aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat
     2641 aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca
     2701 tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat
     2761 aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag
     2821 tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt
     2881 aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg
     2941 atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg
     3001 tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct
     3061 ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc
     3121 aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt
     3181 acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac
     3241 tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct
     3301 tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc
     3361 ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac
     3421 acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt
     3481 caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt
     3541 tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa
     3601 aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat
     3661 ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa
     3721 agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa
     3781 attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg
     3841 gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc
     3901 ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata
     3961 tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag
     4021 agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga
     4081 ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag
     4141 acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg
     4201 gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa
     4261 tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac
     4321 gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc
     4381 cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct
     4441 ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat
     4501 gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga
     4561 aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat
     4621 atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact
     4681 tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa
     4741 cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg
     4801 aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg
     4861 tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa
     4921 gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta
     4981 actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg
     5041 cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga
     5101 tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca
     5161 tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc
     5221 caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc
     5281 tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct
     5341 tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg
     5401 attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct
     5461 gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt
     5521 agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt
     5581 ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata
     5641 gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat
     5701 ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca
     5761 aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc
     5821 actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt
     5881 ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc
     5941 tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc
     6001 cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat
     6061 atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt
     6121 tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca
     6181 aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa
     6241 atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag
     6301 taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa
     6361 agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata
     6421 cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg
     6481 ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct
     6541 tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac
     6601 aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct
     6661 accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa
     6721 attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca
     6781 agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa
     6841 gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc
     6901 agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga
     6961 tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact
     7021 accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat
     7081 caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg
     7141 aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt
     7201 gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct
     7261 taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca
     7321 actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact
     7381 aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca
     7441 tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg
     7501 acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga
     7561 gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca
     7621 aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa
     7681 cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt
     7741 tgtcaacaaa cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca
     7801 ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact
     7861 aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat
     7921 acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc
     7981 accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac
     8041 attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa
     8101 gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact
     8161 cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct
     8221 tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa
     8281 aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca
     8341 tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt
     8401 tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg
     8461 tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc
     8521 ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca
     8581 atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata
     8641 ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac
     8701 aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga
     8761 cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca
     8821 aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt
     8881 tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa
     8941 ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa
     9001 ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata
     9061 tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta
     9121 catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct
     9181 attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg
     9241 agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga
     9301 ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca
     9361 tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac
     9421 tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta
     9481 attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg
     9541 tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc
     9601 cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg
     9661 cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc
     9721 atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt
     9781 actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg
     9841 acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat
     9901 aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa
     9961 aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct
    10021 gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag
    10081 gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa
    10141 agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac
    10201 taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt
    10261 ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa
    10321 tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt
    10381 ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt
    10441 aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg
    10501 atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt
    10561 gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc
    10621 ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta
    10681 atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc
    10741 gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat
    10801 ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag
    10861 agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat
    10921 ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat
    10981 aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac
    11041 tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc
    11101 tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag
    11161 accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata
    11221 ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga
    11281 tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat
    11341 aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga
    11401 ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt
    11461 atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa
    11521 gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag
    11581 gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg
    11641 atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt
    11701 acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg
    11761 ataattaaac tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa
    11821 atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa
    11881 gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat
    11941 caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga
    12001 catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat
    12061 ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca
    12121 agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga
    12181 actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga
    12241 ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa
    12301 agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag
    12361 gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg
    12421 ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg
    12481 atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg
    12541 aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc
    12601 aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc
    12661 cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa ctatataagt
    12721 gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac
    12781 cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact
    12841 aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc
    12901 gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat
    12961 gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa
    13021 ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa
    13081 agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac
    13141 ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag aatatattcg
    13201 taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt
    13261 gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag
    13321 tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt
    13381 agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga
    13441 ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg
    13501 tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt
    13561 tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact
    13621 aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg
    13681 tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag
    13741 tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa
    13801 gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga
    13861 ttcatcaaaa taaaagaaaa caaaaaaagc gaagacttat gtctcgcttt ttttgttttg
    13921 aatgaaaaaa ataaattttt gctataaatc ttttggtaca tagaaacttc ggttgtccat
    13981 accgaaaatc cgatcggtgt attttcctgg ttctgtttga cgaatcgaag tcaacatcat
    14041 ctgcatcgaa gcatcgatat tgtcgatttg atgtaatatt tctgcttcca ttattcttgg
    14101 acgtacaggt gagccatatt ctaataagcc atggtgtgct aaaaccatat gacgtaatac
    14161 gagaacatct tcgtcagctt cgctgatttt caacgcaaga caggctttag ttatttcttc
    14221 gtcaatcaat accagatgac cgatcaaatt tcctgcaagc gtatattctg tcgacatagg
    14281 accggaaagt tcaatcacct ttcctaaatc atgcaaaatg ataccagcat acaacaatgc
    14341 tcgattcagc tcaggatatt catcggcgat tgatttccct aagtgaagca ttgtcaaagt
    14401 atgataagct aatccactgg caaatgcatg atggtttctt tttgctgccg gaaactcaaa
    14461 aaattgtttt tgatatttat taagtagata gcgaacaata cgattccaat gcgcattggt
    14521 gatctcaaac aacaattgat tgatttcttc ttccatttct tctttcttca aaggggctct
    14581 ttccatgtat aaactaggct ctcctggctc atctaccctt gttaagcgca tatgaagaat
    14641 ctttacttga gggtttcctt gataaacctc tcttttaccg tttagaaaaa cgactgtgcc
    14701 ggctgtaaat ttattgattt cctcctccga agcatcccag tatttaccat cgatagtacc
    14761 tgaagtatct tggaaagtaa aagcaataaa tttttttccg tttttagcga ttcttacatc
    14821 tgcacttttg atcaggacaa attgttcaaa ctgctcatcg attactaaat cacgaatttt
    14881 tttcattatt tgctccttct aattgtttta cgatttggtt catctcatgg taatagctta
    14941 ccatttcttt atcagaagaa aaacaaatta tttgatactt ttctccaaat ttagctaaaa
    15001 gttcagctaa ttgatacttt cgctgatgat cataatgaag ccagccatca tcaatgatga
    15061 tcgggcatat cgaacggtta gcttctaatg agaggtaagc aaatctcatc gccataatca
    15121 gctgatcttt cgtacctgta gataactcat agatgggaaa ttctgtatat ccgtctgtta
    15181 aagtcagtgt atcatttttc agtaatatct tctgataatt tccttttgtt aaaataccta
    15241 aataatcacc tgctgctctt aacaactggg gtaattgtct ttcactcatc tctgttgaaa
    15301 gatcacctaa gaatgtcccg gcgatttcaa gtgctcccca ttcctccagt aattcctgta
    15361 attttgcttt ttgccggcta gcttgttgat aaagttcgtc caatgtgccg tctctttgca
    15421 actcttctac ttgaagttgg agtcgttgcc gattttcact tgcttctttt tcttcttgct
    15481 ggagttggga ttgcttttct ttcagttggc gcagtcttac agataatttc tcttttgtca
    15541 gctcttcagg aaatagtgga gtaagcattt ccgataattc attgtatctt ttcatctgct
    15601 gttcggcttg tttggctttt tgtaaaaaga caggtacttc cgaaggataa gataagcctg
    15661 cttgagtcaa ttgttcgctg tattcttgta tccatacttt ttgtttcttt ttcaattgat
    15721 tgatttcttg cttcaataaa gtattttgct gatagcttct ggcaaatttc atctgttcca
    15781 tctcttgtat aaaacgggaa agcaatacca ttttttctga aattgtctta tcttgtatcg
    15841 gtaaccattc tttaacaaac tcaaattttt ggtcgatttg tcgtagtttt tcttgtattt
    15901 cctgttctct tttatgtttc cgctcaaata cgccatataa tttctgataa tctatgactt
    15961 cctgtccata ttctttcatg agtttgagcg tatccatctt tcctaagtgc cgatgatccg
    16021 caaaacgata tacttcttgc tgaagaatct gtttttcttc tccagttctc ttttgttttt
    16081 cttctgcttc agctaattga gctaaggcta gatcaagttg tcctagtttt tcttgccaca
    16141 ttccttttat ctcagagaaa tcatttttag ttttaatttt tggttgcctt ttgtagaaga
    16201 aatagccaat catgcctgca ccaattagta aaaggatcac tttcaagggc attggtacaa
    16261 atatacctgc aatgattatt gctccgccag ccatcagcat atacggttta gatgaagaga
    16321 ctggtgctat ctctgactga ttttgtaata atgctggata ggtctcttcc aagtcattca
    16381 cttctgcttc aatcatctct tgctgttctt tagctatttg gagacgcaca gaaatctggc
    16441 tttctagatc agcaatcctt ctttcttcag ccagaagatg cttccattct tcttcatata
    16501 aaaattcttc cggtaacgga tttttctgcc aatgccacct ttgttccaaa atcttcaatt
    16561 ctgaatttgt ttctctagtc tcaatttgta atctttgcca ttcttcgttt aattgttccg
    16621 ctgtaaattg aagttgctgg agatctttta gttcctgctc ctgctctaaa taaaaataat
    16681 atctggctga ctgctgatcc atcccgctat gcttttccag tgttgcttct aattcttcca
    16741 aacgctcatt tgtttgctga taccgtttgg agaaagactc cagatctaaa agaagttgct
    16801 catcaggaag acctgtagtg ctgttttttt tgagagattg ccattcttca tataaagaaa
    16861 aattaagttc ttgctgtctg gctaatgaaa attgtttgtt cgcctctttt aattgtgcca
    16921 ataactgctg ctgttgcgac tcgtattcct gttcttgttg gatcaacttg ccaaaataag
    16981 cttcctgttc ttgtttttga tgaatttttt cttgcagctc tttccattca tttaattttt
    17041 gattgattag taatttttgg ccttttttct tgaataattg ttgtgctttc tgataatatt
    17101 cttgccgctt ttggaatact tgagaactgc ccgtgatgcc taaagaaatc agcgcatcat
    17161 gtagctcttt ttctctcaaa tgatctagtt ccatcaactg ctcttgctgg aaagtaaata
    17221 cctgctggaa taactctttc gtcaaaggtg cgatcagttt ttctagtaac tcatcacttc
    17281 ccacttgatc ccccagccac accttcgatt ttccgcgatt ttgctgcttg tagcgctcga
    17341 cagcaaattc accatataca ggatgtttaa gccatatttt gccgccatag gctgagccgt
    17401 cttttggtgt ataatccttt tttttacggc cttttgaagg gaaaccaaat aatattgctt
    17461 gaataaattg ataaatcgtt gattttcctg cttcgtttcc cccatagatc agctgattgc
    17521 ctgaataaaa gtcaaaactt ttttgacgcc attttccaaa agcagcaatc tcgatcctta
    17581 aaatcttcat tcctgatcct cctcaaataa aaaatcttga ttaagcagct gcttggcttt
    17641 ttcaagtgtt tcttcttgat attccggcag ttcgctcaat attctagccg cttcttgatg
    17701 actatatact tcatttaaaa tttgttggaa atcttgaggt gtctgatagt attgaaataa
    17761 ctgttccatc aatttagcag aagcagcaag tggggtcttc gtataaaaag tattctctat
    17821 gagagaaata cgccaaataa agaaatcatt tatcctatct gagaattcct ctaacaaata
    17881 gtctaatagc tctcctgatt gtatccgttc caaaacatct gtattgagat ggtcataatc
    17941 ttttaatttt atctcaatta aactaaatcc atcctctact tttgaaagct gctgacgaat
    18001 gtgaacaatg acctcttttg tcgttcttgc tttcctcaat gatatttcct ttgtcttcca
    18061 atagacctct gctactttaa tcggatgtat ttggcaactt cctttactta attcaactag
    18121 tagaatagat gtagaagctt gttccttttt agtatgacct tgaggcgcac ctggataaac
    18181 aattgtttgc ttttcattta agaccgttgg cacatggata tgccctaaag cccaataatc
    18241 gtatcccttt tcttgcatct ttgagggttg gaaaggagca tagtttcctt tttgagagac
    18301 gccaggttcc ccgtggtaca acccaatatg gtaatctgtg agttctctag aaggaaattc
    18361 catcactttg tctttttgaa tccacggttg acgataacta aatccactga gagagacagt
    18421 ttctccactt tttatcgtta ttttttttgt ctctaccgtt tcggatgtaa ataaatggac
    18481 atttttagga aaggagaacc aataccgctc tttttgatag aaatcatgat ttccgaatat
    18541 tataaataca ggtatatggt tcttttctaa ccgtttcatc tgttctgaaa aatgtttctg
    18601 tatttttaat gaaggacgat tttgatgaaa attatctcct gcaagtaaaa caaaatcaac
    18661 gttatttatt attgcctgtt caatgatatt tgataaaacg gtcagattcg tcttcaatag
    18721 tttttcctgt acagttttat caagagttgt cagtccttca aatgaccgat ccatatgtaa
    18781 atctgccgca tggataaatt tcaccattaa aaatttcctc ctttttttct tttatcatac
    18841 cattttcaat gttttttttc atcaataacc gaaaatttgt tcgtattatg caaagaaatt
    18901 acgataaatg gccgacttca aaaaataagt ttattcgaaa aaaagattgt gacatgattt
    18961 ttgtcacaat cctaaatatc tatgttatat tcgcttcgtt tgacactgaa gaagtactat
    19021 ttgtactgga atttatgctc catttttccg tatttagaat tctatgttct atactacctt
    19081 aattagttta tatggtaaga attctttcaa atgcttgaga tactccgtct ttatcatttg
    19141 aaacagtgac ataatcagct tttttcatga tttcttgatg actatttccc attgcaaaac
    19201 taatatctgc actttcaaac atagataagt catttaaact atctccgatc gcgattctca
    19261 aaggtttctt taaattgtag attgattcca aacgatcaaa caccacacct ttagatacat
    19321 tttgaggtac gatttcaata tttacaggtg ccgattcagt aacagacaca tttagcatct
    19381 ttttcaaatg gttaaatata ttagctttca attgacgatt aggaataaaa ataaaaaatt
    19441 tgtaaatgct gatagcatcc ttttctataa tttcctcaat attattataa gggattgaac
    19501 aaatagattt ttcatactca agctcatagt ttagctcttt gatagaaaat tcactcccta
    19561 cctcgtgaaa aatttttttc attttatttt tataattctg agattcataa cttccgagat
    19621 ttgtatatat tttggttgga aatttcgata ggacagaatc aatttttttt acatcatttt
    19681 tatcgaagga agactcaaat atcttttttt ctccatcaaa tccaacagaa ccatttaacc
    19741 ctactgctgg tatccttaaa ccagattttt tgagaatatt attaatgtct tctacttctc
    19801 ttccagaaca aatgaatgga aaacagtctt ccggtaaatg tttcattgtt cttgcatttt
    19861 tttgactaat gctctggtca gaattcaaaa gagtaccgtc taaatctata ccaattagtt
    19921 tcatttcatc catgtctcct atacgttgat tataaattca ctgcttattg taagtctaaa
    19981 tttctcacaa aacatatact aattatacct aatttaagta agtacaaaca tcattttaag
    20041 aataggaaag gacatttata tactattctc tgtatttttg atttataaat aaatatgaat
    20101 tgaatccaaa tcagctatat tactatttta taataaccaa ctataaaaaa cgaagttagg
    20161 tgaaaaacta acttcgatat tcataaaaga ctctattagt tcaacttagc aaaagattta
    20221 aacaaaaaaa gatgaactat ttctagttca tc
//
LOCUS       DACDLI010000047        19894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDLI010000047 DACDLI010000000
VERSION     DACDLI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.047"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(711..1001)
                     /locus_tag="IV353_001782"
     CDS             complement(711..1001)
                     /locus_tag="IV353_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010026.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1209..1901)
                     /locus_tag="IV353_001783"
     CDS             complement(1209..1901)
                     /locus_tag="IV353_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9010027.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            2296..2934
                     /locus_tag="IV353_001784"
     CDS             2296..2934
                     /locus_tag="IV353_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9010028.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(3258..3341)
                     /locus_tag="IV353_001785"
     tRNA            complement(3258..3341)
                     /locus_tag="IV353_001785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3305..3307),aa:Leu,seq:cag)
     gene            3869..4369
                     /locus_tag="IV353_001786"
     CDS             3869..4369
                     /locus_tag="IV353_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9010029.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(4559..4903)
                     /locus_tag="IV353_001787"
     CDS             complement(4559..4903)
                     /locus_tag="IV353_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9010030.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(5057..5977)
                     /locus_tag="IV353_001788"
     CDS             complement(5057..5977)
                     /locus_tag="IV353_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9010031.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            6164..6655
                     /locus_tag="IV353_001789"
     CDS             6164..6655
                     /locus_tag="IV353_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9010032.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(6679..9054)
                     /locus_tag="IV353_001790"
     CDS             complement(6679..9054)
                     /locus_tag="IV353_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9010033.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            9380..10006
                     /locus_tag="IV353_001791"
     CDS             9380..10006
                     /locus_tag="IV353_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9010034.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            10029..10733
                     /locus_tag="IV353_001792"
     CDS             10029..10733
                     /locus_tag="IV353_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9010035.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            10925..11758
                     /locus_tag="IV353_001793"
     CDS             10925..11758
                     /locus_tag="IV353_001793"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9010036.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            11955..13883
                     /locus_tag="IV353_001794"
     CDS             11955..13883
                     /locus_tag="IV353_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9010037.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            14021..14476
                     /locus_tag="IV353_001795"
     CDS             14021..14476
                     /locus_tag="IV353_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9010038.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            14946..15215
                     /locus_tag="IV353_001796"
     CDS             14946..15215
                     /locus_tag="IV353_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010039.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            15615..15869
                     /locus_tag="IV353_001797"
     CDS             15615..15869
                     /locus_tag="IV353_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9010040.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(15958..17004)
                     /gene="holA"
                     /locus_tag="IV353_001798"
     CDS             complement(15958..17004)
                     /gene="holA"
                     /locus_tag="IV353_001798"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9010041.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(17042..19315)
                     /locus_tag="IV353_001799"
     CDS             complement(17042..19315)
                     /locus_tag="IV353_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9010042.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(19308..19847)
                     /locus_tag="IV353_001800"
     CDS             complement(19308..19847)
                     /locus_tag="IV353_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9010043.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
BASE COUNT         6118 a         3816 c         3438 g         6522 t
ORIGIN      
        1 tatactcctt cttatatttc ctaatagagt ataacatagc tttaagcatt acttaaagat
       61 aacttaaaag ttatctttta aatacttaat tgttaaatac cttatttgat gcaaaagaaa
      121 tctacgatag tcaacaaaaa aattttcatt atatccagaa aaatttttct tagttaagga
      181 cctatggatt gcagtaatgc agaattcgtc gaataaactg ccaaagacat acaatcatat
      241 tgcttttaaa attgacgaac aagatataga ttcgtttgtc tcaaaaatcc aaatgttagg
      301 tttaactgtc gaaccaggac gttctcgtgt gaaaggcgag gcttcttcaa tttactttta
      361 cgactatgat aatcacctct tcgagttaca taccggtact ctccaggaac gtttaaaatc
      421 ctacaattcc actacctgag actagtaatg actagtctct ttttatggcc tctgatcccc
      481 ccattgagcg aatactaata gggcttcgaa ttgatctgtt caatctttaa gcaatatccc
      541 tcaaatgagt ttggaaagtt tagtgattca tcacaaatac ttaaatacat gtagttatat
      601 atagattgag gttcttgtga gcgtatgctc gtgtgggttc aaatcccatc gtctgcatcc
      661 atctttccaa aacaagcggc taggacattt ttgtctcagc cgcttgtttt ctagtttatt
      721 ctttcatctt gtcggacctt ttttcttggg tatagacttt tctcatattc taccacaatt
      781 ttgggtttcc ctaacacatg ataggatctc acaacaaatt gctttctatt attataataa
      841 cctgtaacga ctaacttcat cccttcgtct acttcagcta ggaagttcaa actatgggaa
      901 gcaatcaaac aattgacccc atttaaactg aaacggatca acggtctggc actaacttgc
      961 aaagtacgga tcttatctac tgttccatat tttttctcca taaccttcac ctcaatacca
     1021 ttatacgaac atacattccc tttgtaaaga agaaactctg ttttatcttt ttgacttttt
     1081 gtctattacc tttcttaaaa tcatattggc acttcctgcg aagatgaagg acatgtacta
     1141 aaaaaaagag gcagcagtat agattactgt tgcttcttta ctaatttagg tttacttgtt
     1201 aagaaagatt atttacgcaa atgttcttca acaatttgct gagatttatc agctgtatct
     1261 tccacttcat cttttgcttt ttctaacttt tgaatatatt gtttcaagct tgagttttca
     1321 gcagttgctt cgtctaattt cttttgtgta tctgttaact gagtttctac atttgttttt
     1381 tcttgtgtca gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa
     1441 agttttgctt gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca
     1501 tcattttttt ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt
     1561 tgtgcatcct tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt
     1621 ttttggtttt ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct
     1681 tgcaatgtct gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta
     1741 tctgtcaatg tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct
     1801 ccaccccaca aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga
     1861 aatgcaacga caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc
     1921 aatactttta catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta
     1981 catatgtaat tttaatataa atactatcaa acactcattt aatcctcctt ttttatctat
     2041 ttaaaaaatc ttttttatag agaaaaatgc aggttaatac tttcttaaat aaatatttat
     2101 ggtgaacaaa aacaaaaaag caagttttat taaacgtaac ccttttcatt tcatagatta
     2161 ctgtatttat tacaaaaaac gcggatttgt cataaattgt catgcttctt ttacctatga
     2221 aatattttca cgtgttaaag tattcctcgt tgaacacaac aaataaaact ttttaacgaa
     2281 taaaggagat ttttcatgac atcacttaaa actttacttt ttggaacaac tttggctgcc
     2341 ggcgctgcat tcttcatggg aacgactgct catgcggacg aagcttatac cgttcaatca
     2401 ggtgatactc tatcaacgat ttctcaaaaa tacgttggtg ataactcctt gatcaatgcc
     2461 attgcagaat caaattcaat ttctgatatt aacctgatct actcaggaca acaattaaca
     2521 atcccaacag aaggctctgc acaagcagct gctgaacctc aagcagccgt tcaagaagca
     2581 ccagtccaag ctgaacctgt tcaagctgaa caacctgttg tacaagaaac ggttcaaacg
     2641 gaaacacaag cagcgcctgt tgctgaaaca caacctgctc ctgctgtaac agaaactgct
     2701 gctactccag caagtacaag ctctgcaaaa gaatggatcg cacaaaaaga atcaagcggt
     2761 tcttacacag caacgaacgg acgttacatc ggccgttacc aattagattc ttcttactta
     2821 aatggtgact actctgctgc aaaccaagaa agagtagcag aacaatatgt tacttcacgt
     2881 tatggttctt gggaagcggc gaaagcattc tgggaagcaa acggttggta ctaagattca
     2941 ttatttgaac gtttaaacac attcaaaata tagtaagagg tcatgcgatt tattctttcg
     3001 catgacctct ttttgagttt aagtttagtc tttattcctt aactatcgtt tgccagtatt
     3061 tcttgtatca gaatctataa atctactatt tgaatcaatc tattaggttt taaactgtcc
     3121 caccttcaca caaatttttt ttgatttatt tgaatctggt tccgttgttt aaaatttttc
     3181 ctatattctt ttagataata tgttattttc tttctcatcg ctccaggtgt accttctctt
     3241 tataaaaaaa caaattatgc ggctaagaag actcgaactt ccacgaggtc accctcacta
     3301 gcgcctgaag ctagcgcgtc tgccaattcc gccatagccg catcacatct tttagtttag
     3361 gattacatgt gttttttgtc aagaacatat ttttgttctt tttcatttct gtacgccctc
     3421 ccttatgcca tctacacaaa taattcatcc gagcaactcc atcatctatt gagggttggt
     3481 gaaaaaatct gttttatcca tatgatcgat ttcaatgtta aacgaacgac tctgggatta
     3541 gtaactattt tatttttttc ttcatttctt ttttcctctt tttctcgatt cagctatctt
     3601 ctttcatttg gatattgatt atttcttctg ttgattacta tccttctgga attctagacg
     3661 ctgaagtaat tcacgttttc tttcttctag agatcataag taacgaatga acgaacttca
     3721 aataattttt ttattgtaat tcatatttgt attgttcgat gattcttaat acgatatgac
     3781 acaataaata aaaataggca tggctattta caacaatagt gctatagtag aaaacaaatg
     3841 aataaggtta cttaaagagg tgaatacgat gaaactgatc aaactatggg aagcagattt
     3901 aaagaaagct tatcagctac aaactagctt tggaccaaat gagagtggat ttatcaacaa
     3961 tgcttatggc tatacatttg ctgaattttt agattatgta aaaaaatgcg aaaaaacgtc
     4021 tttaggaatc gatcttcctg agagttacgt tcccgcaacc gtctttattt tagaaagtga
     4081 tgaacatgag tatgtaggaa tttttaattt gcgccataaa ctgaacgaaa cattaagaaa
     4141 cggcgctggc catattggtt acggcatctc ccctaattat cgtaaaaaag gatatgccac
     4201 acaaggatta gcattagtac tacaagaggc aaaaaaaatc ggcattgatg aggcactttt
     4261 gtctgtaaat aaaaaaaatc ttggcagtct taaagcacaa caaaaaaatg gggctgcgat
     4321 ttatcgtgaa gatgagaaag aatactatac gaaaatcaaa ctgatgtaaa aaagaaggat
     4381 tccaaaagga aagggatttg aatgtccttt atcaatagaa cacatttatt taattaactt
     4441 ataatatagc agacaaaatt ttataaacaa agaaaccttt tatctctttt cttctagaaa
     4501 ggataaaagg cttttttgtt tttcctattt gcttctcata gctagtcagc caagatttct
     4561 atcgtatccc caacgcaatc cgtgcataac ggctcatctt atctgtcgtc catgctggat
     4621 accagactag tttgacttct gctttcgtta cttctggcac ttcttttaat gcgtcatgga
     4681 tagattctgt taaaatatct gctaatggac agcccatagt tgttagagtc atcttaatga
     4741 ctgtttctcc attttcagga ttgaattcta tatcataaat caagcctaaa ttgacgatgt
     4801 cgattcctaa ttcaggatca atcaccattt ccaaggcagc aagaatacgt tctttgatta
     4861 gttctatttc ttgtccagac cgttcttggt ttgcttcgct catcgtacga cactccttaa
     4921 cacgattttt atttatcata ctcttttttt agctgcttgt aaatgagaat ttagaagtaa
     4981 gattctcgtt taaatttata aaaatgttct tattagatgt aatagattaa atttattcta
     5041 aaataaacct gattcctcat tttttttgtg aacgattttt tatttctggc aatacgccag
     5101 ttgttcgata cattttttgt tcgttttcat ttgcaaaccg gccaaatact gaatgagttg
     5161 gtaaaatatt gataaatgca tcgtcgtcga tttcatgaac gatctgatca agatcataca
     5221 tttcgtagcg ggtgatgacc atcatgatca tgttcccttg tttttttgaa tagccaccga
     5281 cagacggcag taatgtcatt ccacgtacca tccgttttgc tagttcttct gttacctctt
     5341 ctggcttgac ggtaacgatc attgctgtta ctttttgatg actcgtgtgg atggcatcaa
     5401 ttacttgagt cattgcataa atggaaataa tcgtatacaa ggcactttcc cagttaaaga
     5461 agaagcctgc gatgactaca atgataccat ttagaatcag catgtagttt ccgactgttt
     5521 ttccagttgt ttgtgaaagt acaagagaga tgatatccat cccaccagtt gtaaagccca
     5581 tttttaaaga taatcctacg ccaagaccta ataaaacacc gcctactagg gcattcatca
     5641 aaatattatc agtgatctgt ccgactggaa gcagcatcgt catcaccgat acacatacaa
     5701 cattcacaaa actaaggatc gttgctcgtt ttcctacttt gagaaaccct aatacaaaca
     5761 cagggatatt caaaagaaaa ataaatgttc ctgtgtctat cttgatatga aaccattcat
     5821 ataaaagcga ggaaatgatt tgagcgatcc cattcatccc tgcagaaaag acattggctg
     5881 gaatcaaaaa ataatttaaa gcgaccgcag cggtcaatcc ggttatcaaa ataaccacta
     5941 ttttctttaa tctttcattt ttatgatacg cagtcataat tttactcaca agcacctctc
     6001 ccctactttg gtattccaaa tgtaagaata acagtttatc tattaaaata aaatacttcg
     6061 aactcaaatg agaagaattt tagcaaacag gtaagaatct gccctaatcc tatgataaga
     6121 tagatgtatg gtaaatattt atatgtatat caggagggtt cctatggcaa aagatgctag
     6181 cttcgacatt gtatcagaag tcaatacaga agaagtaaaa aatgcgattc agcaaacaac
     6241 aaaagaattg tccaatcgct ttgacttcaa ggattctaca gtagaaatca aattagagaa
     6301 cagcaatcta gttgtattga gcgatgatga tttcaaaatc gaacagatca aagatgtatt
     6361 atttggtaag ctgatcaaaa gaaatgtgcc aatcaaaaat atccattttt ccgaatcaga
     6421 acacgctcta ggtggcaaag ctcgacaaac tgccgatttg gtcaacggaa tcgatcgtga
     6481 aaacgcgaag aaaatcacta cagctatcaa aaatgaaaaa ctgaaagtaa aagcacaaat
     6541 ccaagaagat cagattagag tgacaggtaa gaaccgtgat gatctacaag ctgtgattgc
     6601 tttattacgt aaacttgact taccaatcga gttgcaattc actaactatc gttaatcaaa
     6661 aaacgaggct gacgtctgtc attcctcgtt tttttgattt actgcttctt ttgataacca
     6721 aaaacgctgt attccttctt ccaattcccc aacacttcgt acttctcttg ctgtttgcca
     6781 atgttgtggg aataaacgtc gataagaaaa actagaaaat cgttgatctg ccaatacaac
     6841 gattccttga tccgccatgc cacgaatcac tcgaccacca gcttgcaaga ctttgttcat
     6901 tccaggaagc tgataagcgt aagcaaatcc aaggtgttcc ttctcatcat aatatgattt
     6961 gatcagttcc tgctcatgat tcatctgcgg caaacctaca cctacgatca tactgccgat
     7021 caatcggtcc cctctcaaat cgattccttc tgaaaaaatc ccgccaagta cgcaaaatcc
     7081 aatcaatgtt tcagcgggat ttgtttgaaa ttttgttaaa aatcgttcgc gttcttcctc
     7141 attcatgtcc gtttcttgga ttagcacatt gctgtcggga tattgctgtc tgaatgcttc
     7201 caccgcaaga tctaaatatt gataagaagg aaagaaaacg aaataattcc cgacctttcc
     7261 atcagcaaaa gcatgaatcg tctctactaa ttttgccaga ctgtgttccc tttttcgata
     7321 agtcgtctct aggtaatttg cgatgaaaac ttgctggttt ccttctggga aaggactcgg
     7381 cagtttgtaa gccagacttt tttgtccccc cagtgtttcc tgataataag agagtggtga
     7441 aaaactagca gaaaaaagaa tactgctttt tcctttatcc aacgactgct caagaaacaa
     7501 gcttggatca atacaaaact cttttactat cagatcgcga tacgtttttt cgactgttgt
     7561 ttcgtagtga tcatcataaa attcactgac cttcaaaaaa tgaagcaaat caaaataata
     7621 aggaagcaat tgttcttgtg atgggtgctc aggaaattct gccagccatt ctttaatttt
     7681 ttcgcttaat tgataacctg cctttactaa agattcagcc ggtgcttttt gatgatgata
     7741 atcccaatga tcttcttttg ctatttctcg gatcgaatca aattctttta atagtttgtt
     7801 gaaacggcga tgaagttttt tgaattcctt tggtatagct tccttacttc gtttcgtttt
     7861 ttcataagat agttctgccg aatacatctc tcgggaacga ttcaccaaat tatgtgcttc
     7921 gtcaatcaaa aacagatagt cctcgttttt ttcatcttca aaaaaacgtc ttaaataaac
     7981 agttggatca aataaataat tgtaatcccc aatgattacg tcacaccaga tactgacatc
     8041 taatgatagt tcgaaaggac aaagtgtatg ctttcttgca taagtttcga tgacttcacg
     8101 agtgatctga ttctcatgat tcaaaagatc ccataaacct tcattaatcc gattgtaata
     8161 accattggca tatgggcact gatctggatt acaggtcgtc tcatctaaaa agcagatctt
     8221 gtctttcgcc gtcaaagtga cactttttac ttcagctcct gtatctttta atgctgtcat
     8281 agcatcttca gctacttgtc tagttattgt tttagccgtt agataaaaga tcttttctcc
     8341 ctcttcttcc cctacagctt tcaaagcggg gaataacgta gagattgttt ttcctgttcc
     8401 ggtaggtgct tctacaaaca atttctgttt cgtacggatc gttttataag cagctaccgc
     8461 tagttcacgc tgacctttcc tatactcatc atgggggaat ttcaaagcca tcaaagaggc
     8521 attgcgtaca gtgcgccagt tttcttgaaa aacaagccat tctttataat cttcagtcag
     8581 ctttttaaaa aaagtttcta actgctctct tgtctgatgt tcaacctttc tcgtgatcac
     8641 ttcttctgcc gtctggaagt aagtcaattg cagatttatt tcttgtaaat catgttcttg
     8701 actatagata taggcataca cccttgcttg atgaaagaaa agttccactt gttctggttc
     8761 taaatcttca aatcgtggtt cggaggtttt gatttcatct atataataaa taccgtctct
     8821 tttgaaaata ccgtctgcac ggccttcaac gatcaggtaa tattcatcta attgaactgt
     8881 tgtttttaaa aagacttctt tttcatagtt ttctcccgca tctttttgga gcttgcggtg
     8941 gattctcgct ccttctaatg cagtatggtt acttttttta cgattgtcga tacttccacg
     9001 tcgcaaaata aattctacta agtgccgcac agatattttt gactgtttga tcatttccac
     9061 cacctctttc tctctaagat tacctgaaaa aacctagttt agcaaaaaga gctctctttc
     9121 caaacataaa aaaacaccct gctgattagg cagggatata ggatatagaa tttggatttt
     9181 tggatgaatc taatgtaaca aggacatatt aaaatgctat gaaaacttac tttccttctc
     9241 tttagaatat ccaaatactt ttcatatagt ctgataattc agataagtaa agagaaataa
     9301 gtacctgttt ttgtttccta gcaatgaatg cgttatacta atggatgaaa gaagttttgg
     9361 ctatcaagga ggaatgcaca tgcatatcga aagattaaaa acaggaatga ttgaagaaaa
     9421 ctgttatttg gtctacaacg aagaagcact tttgatcatt gaccctggag aagatgcgga
     9481 acgaatcaaa caagaaatca aaaaaacaaa ccaacagccg gtagcgattt tgcttaccca
     9541 tactcactac gatcatatcg gtgcagtgga acctttacgc gcgtattatc aaatcccagt
     9601 ttatgtcagc ccactagagc aaaaatggct aggtgatccg attttgaatc tttctggact
     9661 cggtagacat gatgatattg ctgatgttat tgtctcgcct gcagaatatg aatttgaaat
     9721 gaaaacttat actttaggaa acatgtcttt tcgcgtcgtc cctaccccag ggcattctat
     9781 aggaagcgtc agctttattt ttgatgattt tgtcgtttct ggcgacgccc tgtttagagg
     9841 aagtatcggt agaaccgatc tgtatacagg gaatttagaa caattacttc atagtattcg
     9901 gacccagctg tttgtattac ctgatgaatt tgcagtctat ccaggacatg gcgatgcaac
     9961 gacgattgaa caggaaaaaa ggacaaatcc ttttttcaat cgttaaaacg acttaggagg
    10021 agaataaaat gacagaaact acactttata tcgtacgaca cggaaaaacg atgttcaata
    10081 caattgaacg agtacaaggc tggtgtgata cacccctcac aaagcaagga caagaaggca
    10141 ttcattattt aggtcgcggg ttaaaaaatg tcgacttttc atttgcttat tctagtgaca
    10201 gtggtcgtgc aatcgaaact gcccgtatta ttctaggaga acattctaag ggaaaagaaa
    10261 ttccttattt tattgaaaaa aggattagag aatggtgttt cgggtctctt gaaggtggat
    10321 atgacatgga aatgtggggt gtgatccctc gtgtattgaa ttttaaaaca tatgatgaaa
    10381 tgtttaccac agaagtttct tttgaacaaa ttgctaatgc tgtatacaac ttagataccg
    10441 ctgggtgggc ggaaacttat gaaacattgc gagaccgtgt atggacagga tttgaagata
    10501 tcgcccatca ctgcgaaaaa aatggtggtg gaaatgtttt agtcgtatct catggtttga
    10561 cgatcgcctt tttgcttaat ctgatcgatc ctaaacagcc tgttcgtgct gggcttttaa
    10621 atggcagcgt aacaaaggtc acttatgcaa acggtcgatt ttctatccac ggcatcaatg
    10681 atacgtctta tatcgataac gggaaaacac atagtcgcga ctcgtttcta taatggggat
    10741 attttaattt ttaccagaga atcataaaag gcaaagtctg aacgagggct ttgtcttctt
    10801 ttttctcttc gtgtacttat ctgctttatt tctcaagttt ttcttaatat taaaaaacag
    10861 attgggtttc tcaaaaaata acggtatact ataagatgta tcttaattgg gaaggaagga
    10921 atttatgttt tttgcaaata ttatcaaagc agtgattctc ggtattattg aaggaataac
    10981 ggaatggctg cccatcagta gtactggtca tttgatccta gcagatgaat tcatcaaact
    11041 ggatgccagt tcacaattta tgtcgatgtt caatgtcgtt atccaattag gggcgatctt
    11101 ggctgtcgtc gttctttatt ttcacaagtt aaacccattt tctccaagta aatcaacggt
    11161 agaaaaaaaa gatacgtgga cactttggtt taaagtgatc gttgcttgca taccagccat
    11221 catcattggt ttgcctttgg atgattggtt agaagcaaat ttccacaagt tcttaccagt
    11281 tgccattgtt ttgatcgttt acggtattgc ctttattatt gtcgaaaaac gcaacaaaaa
    11341 caaaacacct cgttggactt ctctgaatga ctttacgtac caagcagctc ttcttgttgg
    11401 gttattccaa gtcttggcac ttgttcccgg aacatcccgt tctggcgcaa cgatcctagg
    11461 atctatcttg attggtgctt cgcgttttgt cgctacagag ttttcatttt tcttagggat
    11521 tcctgtcatg tttggcgcaa gcggcttgaa gatcgtcaaa tatttagctg aagggaattc
    11581 attcaaaatg gaagaaactg ttgttcttct tgtaggaaca ttcgtatctt ttgtggtttc
    11641 cgtcttagct atcaaattct tactaagcta tttgaagaaa aacgacttta ccgcttttgg
    11701 ctggtatcgt attatccttg gtatcatttt gattggttat tggtttattg caatgtagaa
    11761 aaaaccagaa cacttctctt atttgagaaa tgttctggtt ttttattttc tttaataata
    11821 atcattataa ataataaaaa caacatcaag cttaatcacc aaatgataac cattatcgtt
    11881 ctcatttagc tgttattatt atcaattaca tttttattat gctatccttg ttttgacaag
    11941 gaggtctaat aacgatgact aatcagaaaa aagcttttgc agcaacaatc ggctgccttt
    12001 tatttatgat cttcggtttt atcttcgacc gattctctct taccatcacc ccactttttt
    12061 atatcagtgc cattatcatc ggtggatata aacaaacatc ggaaggcttg aaagaattgt
    12121 ggaatgatcg gacactgaat gtggatttat tgatggcttt agctgctatt ggtgcctgtt
    12181 tgatcggtaa ttactttgaa ggagcaatgt tgacattcat cttttgttta agcggtgcat
    12241 tagaggaata cacaactaat aaaagtcaaa aagaaattac tgctttgatg aatcttcaac
    12301 cacaaaaagc ccaattatta aaagaaaatg gacaaatgca agaagtagat gtttctcaac
    12361 taaaggtgga taacttggta tttgtcgcta aaggagcagc tgttccgata gatggctttt
    12421 tagaaagcac acaggcaacg atcgatgagt ctgcattaag cggtgaatct gtgcctgtag
    12481 aaaaatattt aggcgatgag atgtatgcag gaacgctgaa tcaaggaaat cccatcacga
    12541 ttcgagtaac aaaaacgagt tccgaaactg tctttgctaa gattatccaa ttagtagaag
    12601 aagcgcagaa tacgccaact caaacagctt catttatcga acgaatcgaa aataattatg
    12661 taaaattgat tttgcttgct gttcctctaa tgatccttct cccccacttt ttcctaggtt
    12721 ggagttggga tgaaagcttt tatcgaggaa tggtattatt agttgtggct tctccttgtg
    12781 cgcttgtcgc atctgctaca ccagcttctc tagctgctct ttcaaattct gcaaaaaatg
    12841 ggattttaat caaaggcggt atccatttag aaaagctttc tgaattaaaa gcgattgcct
    12901 ttgataaaac aggaacgctc actaaaggaa aaccagttgt aactgacagc tttttctttg
    12961 atgaaaagga tttggcacaa caacttcttg ttgctatgga acaaaaaacg acgcatccac
    13021 ttgctcatgc gattttgcaa tatttcgatt gcacaatacc tgaagaaatc caatacttac
    13081 caatagaaga aattactggt tttggattac aaactaccta tttaggtgcg caatggaaag
    13141 ttggcaagca cgcatatgat cctgagacga tgataatcag caaagagatt gcggaaatga
    13201 tcgaacgttt ggaaaatcaa ggcaaaaccg tgatttatct ttctaaagat cagcagttaa
    13261 ttgctgtttt aggtctgcta gatattccaa aagcaaatac ccaacaagta atttcctact
    13321 ttaaatccca aaacatccat acaagtatga taaccggcga ccattctggc acagcaaagg
    13381 ccattgctga acaagtaggg attgaacatt actatgcaag ctgtactccg gaagaaaaaa
    13441 cacaactagt caaaaaagag aatgaacttt accaagtaaa tgcgatggtt ggtgatggag
    13501 tcaatgatgc gcctgcttta gcagccgctt ctttaggtgt cgctatgggg caagggacag
    13561 atgccgctat ggatatcgca gacatcgtct taatgaaaag cgacttaaat aaatttgtct
    13621 acagtcatcg gctatcttta aaaatgcggc ggatcatcaa acaaaatatt attttttcga
    13681 ttcttgttat tctcttgttg attgcttcta acttcctgca atttttaaat ctacctcttg
    13741 gagttgtagg acacgaagga agcacaatat tggttattct gaatggcttg cgtttactaa
    13801 gacctttacc tgatctttct gattcttcaa aaaaatgtaa gtcttgtcca ctatacaaag
    13861 cgcggctgtc ttctgtcaaa taagcaaagt gtttttttat gattttgttt attttatgaa
    13921 aaaaacagct tatgaatagg tcttgaagga tgaattccta cttttgtaac tttctaaaaa
    13981 catgttatga ttgtttcgaa gaaagtgagg aatatatttc atggaaagtt ggcttttttt
    14041 aggaattatt ttacttgtag caattttagc taaaaatcaa agtctgatga tagcggcagc
    14101 ttttgttttg attatgaagc tcataccagc ctcccgttcc tttttccctt ggcttcaagg
    14161 caaaggaatc aatattgggg tgacgatcat cacagcggct attctaatcc ctatcgcaac
    14221 aggagaaatc ggtctacgtg atctattaga agcctttaaa tcccctattg gctgggtagc
    14281 tgttttttgc ggtgggctag tggctgtctt atcttccaag ggtgtaggat tgattgcttc
    14341 tgatccacaa atcactgttg cacttgtttt cggcacgatt ttaggtgttg tcttcttaag
    14401 aggaatcgcc gccggaccgg tcattgcttc tggtatcact tactgtatct tacaagtatt
    14461 tgctttagga aaatagaaca aattttgtct attttaggag attcttaaca cccccaattt
    14521 actgatccgt gatatactga caacgaggtg atacaatgtt cgaaaaatta aagtatttca
    14581 taggaacaat cggctggttt cttctagcgg tacttttgat tctgggctta gcttatctat
    14641 tatttcctga tttgatacaa aatgtgtttg atactgcttt ttgattgacc tattaaaacg
    14701 atatagggga tgctcctgcg atgcaggggc tttttttaat caatgttttg taataattta
    14761 ggccgtgcca atgagacttc ctatagaatt cgatgctctt ttttcttttt ggctcttctg
    14821 ctcttttttg ttatactaga ttacagtgga caaaagaaag aatgatcgca tccacttcaa
    14881 cgaatatcaa aatggaagaa ttagtgcctg ttttcaggtt actttgaaaa gaaaggaaga
    14941 cgcttgtgga aacttttgaa ggtcttttaa ttgatacgta cgttttacaa gaacagccaa
    15001 tgatgctgac tgggatcaac ttgcagcttg aggaccgtgt gatcaatggc ctgcttaaag
    15061 atcgctattt ggcaaaatat atcttacgtt tgcccctaaa taaatatgtg ctggaagtaa
    15121 gtggcaaatg gaaccgtcgg aaccagttgg tcataaaaaa aatgttgata aaaaatatcg
    15181 atgaatacat taaaataatc ggtacaccag aatagccttt actgatcaaa ggatactggt
    15241 tataaaataa ctgcagtttc ttaatagaaa aacaaagaac aacagggaat cgtatccctt
    15301 ttcaaaaaaa actttgcaaa cggttctctt tgttatataa taacttagta ccaaacattc
    15361 ccattcaaac tttgtttaaa tagataaata aaaatctagt ttggtttgac gctattgcct
    15421 cccacaatag cgtctttttg atttttttca gcttgtacta tccgttttta ctgaaaaaaa
    15481 atcctaataa aagactgtta aggaaaaaaa cttgtcatgt tcatattgca aagcacgctg
    15541 cctttaggta taataatgaa tgttgaatta tgcggtaaca cgcgtaagaa cttaagttat
    15601 cggaggtgaa actaatgcca aacattgaat ctgcaatcaa acgtgttcgt acaagcgaaa
    15661 acgctaatgt gaaaaattca tctcaaacaa gcgctatgcg tactgcaatc aaaaaatttg
    15721 aagatgctgt agcttcaggt gctgacaacg tagatgcgtt atataaagaa gctgttaaag
    15781 ctattgatat ggctgaatca aaaggtctaa tccacaaaaa caaagctaac cgcgacaaat
    15841 ctcgtttaag caaaaaaatc gctaaataaa agaatcggct tcggccgatt tttttatttg
    15901 tcaaaaaaag gacaaaaaca aacgaccttc ccgcatgctt tgccctcgtc tttcttctta
    15961 tcgatttctt aacaactgcc ctgtgcgcaa aacgaatagt tgaaaactta attctttttc
    16021 gatttgtccc gttttgattt gataatccat ctcaactaat tgatcaaaca actgtaccag
    16081 caaatgttca tcgaattttt ttacttcctg catggcaagt tttacacgat atggatggat
    16141 tttcaatgtt tcagcaatat ttgcctgctg ataaccaatc tttacaagaa attttgtctg
    16201 taaaaataaa cgtaattgag aaagcaaaat cgcattaatt ttgattgttt cttccccttg
    16261 cataactaaa tcttcgtata aacgcaaagc ttgttccgtc ttccctttta aaatatattg
    16321 ggtcatgtca aaaatattat gttctaacgt tttaggaact aattcttcta cttctcttac
    16381 cgtgatttgc tgttgatttt gtccataaag gaccaatttc tgtaactctc tcatcatttt
    16441 ggataaatct aagtcggtca acctcaagaa taaatcaatt gcacttcgtt ccattttgat
    16501 tgaggaattc tctaaggtat tcatcagata ttgacgtact tctttttcat tcaactgttg
    16561 gacattgatg ataattgctg tttttttcaa ttgtttcgtt acttttttcc gttcatccaa
    16621 cttttcatat ggcgcaaaga atactaatac agtcgattct aatggttctt tcaaataagc
    16681 cgtcaataaa tcagtatttt gttcgattcc attgtttact ttttcacctg ttaaaaaata
    16741 aggattctct acgaatacta accgttgatc tccgaaaaac ggcaacgtct ctgcttcagc
    16801 tactacctca tctaaacttg cattttccat gtcaaaactt aaaaaattta agtcatcttc
    16861 gccctctaat tggatttttt tgacaatttc tgtccgaact tgatcttgga gaaacttttc
    16921 tgttcctaaa atcaagtaac aagatgcaag tttttcttta cgtatttttt gtaactctgc
    16981 ttgtatactc aaattactca ccactttcaa aaattatcct accatgagca gatgagaatt
    17041 actagtctat cattgtttcc actagcccat gaggatgaaa aactggatgc cagcgatagg
    17101 tgatcattcc ttgttgatcc gttcgccatg tttttatctg attttcttct agtgtctcca
    17161 atacttcgct gtgcgggtga ccataacgat tatttacgcc gcaggatata atcccatgtt
    17221 ttgcttcaat atgggaaaga aaatctgtgt tacttgacgt acggctaccg tgatgaccta
    17281 atttgactac atcggctttc agattaggat aaatcaaaag caattgtttt tccattgctt
    17341 gattcaaatc tcctaaaaat aaaaagcgcc tattgttata ttccatccat agaccaatcg
    17401 aatcttcatt tcttccctct cccttttcaa aaggagtcaa tacataaaga tcataataac
    17461 cgctgatcct gtccccatgt ttgatttcaa tgatctcagt accatttttt tgagcttcag
    17521 ataaatgcct ttgtaatggc tcatttcgtc cagcgcccca accgatataa atttttttga
    17581 tagtaaaatg ctgagataac gtgggtaatt ctcccacatg atccgtatcg tcgtgtgtca
    17641 agatgagttt atcgattaca cttatcccct gaccttttag atatggaacg atatttcttt
    17701 cagcaggagg acgtatcata ccttgcgccc acttttctct ttcaaagtgt aactgacctc
    17761 cagtatcgat caaaatcgtt tcttttcgaa aaggactttg caaaaagata ctgtctcctt
    17821 gaccgacatc gataaaagcg atcgttgaag aaaagcgtaa aaatggcaaa gtcaaaaaaa
    17881 taaaagaaaa aagaccaaca taaaacaagt gtttcacagg ttgttctttc aacctatcca
    17941 tacaaatcag taatccgaaa atatacatcc cagatagcac aaggggcaaa cgtcctataa
    18001 tgagagacga ccctgaagaa aaatcgatta ttcgctcaaa taagattaat ccctcttcta
    18061 tcagcaaaag taaagtttcc ggcagccagc gattgaagag tgtgagaaat aacacaccag
    18121 gaagaaaaag aaacttgaat accggtgcaa tcagtaatgt aaaaagacta cctaaaatag
    18181 gccactcata aaaatgccat acaatcaacg gcatcgtgat catagtaaac aggacgtgag
    18241 aggccgtttt tttcaaccaa tctccatcaa aagaaatcag gataaacaaa aaactcatga
    18301 aaaaactaag ctgtccaccc gcatgaaaaa gaaatcgagg atcaaatagt aaaagaagcc
    18361 aaaagacaac tgcaaataag tccatggtgg aataatgctt attcagcaat ttgactagca
    18421 atcgcaataa aaacagcaaa ctagcacgca aaacagaaat agaacttcct gcaatagaaa
    18481 cataacagat agttagtaga ctcaacggaa taatactgac gtgtagtggc catccgatac
    18541 gtcgaaataa ataatacagc caccctaaat aaaattggat gtgtagaccc gatagactga
    18601 acagatgcag caaaccactc tccctaaaaa tttcttctgg ttctctgaat cctgcatctt
    18661 tatagcccca taataaagaa ttaatatata cacttgtttt ttgcggaaat tcagattgga
    18721 caaaggagat aaaagatcct ctttttctct gaagccagtt tcgccagtga ttttgcatgg
    18781 tgatctgttc aatcttttga atttggaacc gtccagaaat tctatggttc ctgaagtaag
    18841 cttcttggtc aaaattataa agattccgct tttttgctgg agagatgaac tcgccttcag
    18901 cagtcaattc tattcccttt cctcggaagt taagccatgt tttcaattct gcttctgtgt
    18961 ttacttgata agaaagctgg agcttttgtt tattctctga taaccttcct tccattgtca
    19021 aaaaattacc atttgcacga atcgtatctg taaatacagt aatttgcgca gatgttgttt
    19081 tttcctctaa aaatgaatac tcttgtagcc tataagaaat ggataccgtt atgctacaca
    19141 aaatacacaa gtacgcgata aaacgtcctt cttttagcga aaaccataaa aaaattccgg
    19201 caagaagcaa acgataaccc caattgtcag tgtaatacaa caaacagata atcatcgacg
    19261 taattgccgg aaaaatcaac cgatttcgaa catgcctttc taaaacgcta gacatgtttt
    19321 cctgttaatt cattttgaag ttttgaaaaa tgctgtgagt ctaaagcaac ttggtgtaca
    19381 gatacaccta attgttcgat caacgcttgg gcataaggat catttcgata gtcatggaga
    19441 taattgatcc ctttcacacc agcttgcaac aaagcctttg tacaggccaa acatggaaaa
    19501 tgtgtgacat agatttctga accttccgtc gggataccaa gttttgcaca ctgtaggagt
    19561 gcattcatct ctgcatggat cgttcgcaag cagtgtccat ccacgacgta acacccttca
    19621 tctatgcagt ggacatctcc actgactgct ccattatagc cgccagcaat gatccttttt
    19681 tctcgaacga gtgtcgctcc tacttctaaa cgtgtacacg tactacgtaa agataacagt
    19741 actgcctgag ccataaagta ctggtcccaa ggaatgcgtt cattatgtga cttatcagta
    19801 actttttctt tattcgtatc gcatgctttt gaagattttt ctaacatgtt ttatattcct
    19861 ccgttttttc aagttgatta aaaagtactc attg
//
LOCUS       DACDLI010000048        19398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDLI010000048 DACDLI010000000
VERSION     DACDLI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.048"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            881..2131
                     /locus_tag="IV353_001801"
     CDS             881..2131
                     /locus_tag="IV353_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9010044.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2150..3160
                     /locus_tag="IV353_001802"
     CDS             2150..3160
                     /locus_tag="IV353_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP9010045.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISTERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            3170..3823
                     /locus_tag="IV353_001803"
     CDS             3170..3823
                     /locus_tag="IV353_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9010046.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3842..4840
                     /locus_tag="IV353_001804"
     CDS             3842..4840
                     /locus_tag="IV353_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9010047.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            4916..5677
                     /locus_tag="IV353_001805"
     CDS             4916..5677
                     /locus_tag="IV353_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP9010048.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5720..6802
                     /locus_tag="IV353_001806"
     CDS             5720..6802
                     /locus_tag="IV353_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9010049.1"
                     /translation="MTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVKSDEEVGTE
                     QLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSWTVKNGIKY
                     IPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQLGVNKVKLA
                     PVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMISLFNKLDE
                     HNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVFVNLNYTEI
                     WGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGRFTNDIIDA
                     AYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            7224..7679
                     /locus_tag="IV353_001807"
     CDS             7224..7679
                     /locus_tag="IV353_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP9010050.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7693..8466
                     /locus_tag="IV353_001808"
     CDS             7693..8466
                     /locus_tag="IV353_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9010051.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8453..9583
                     /locus_tag="IV353_001809"
     CDS             8453..9583
                     /locus_tag="IV353_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9010052.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9614..10849
                     /locus_tag="IV353_001810"
     CDS             9614..10849
                     /locus_tag="IV353_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010053.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10874..11983
                     /locus_tag="IV353_001811"
     CDS             10874..11983
                     /locus_tag="IV353_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010054.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            12025..13359
                     /gene="gmhB"
                     /locus_tag="IV353_001812"
     CDS             12025..13359
                     /gene="gmhB"
                     /locus_tag="IV353_001812"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP9010055.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13394..15031
                     /locus_tag="IV353_001813"
     CDS             13394..15031
                     /locus_tag="IV353_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9010056.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15133..15528
                     /locus_tag="IV353_001814"
     CDS             15133..15528
                     /locus_tag="IV353_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9010057.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15546..16277
                     /locus_tag="IV353_001815"
     CDS             15546..16277
                     /locus_tag="IV353_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010058.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16301..17752
                     /locus_tag="IV353_001816"
     CDS             16301..17752
                     /locus_tag="IV353_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9010059.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17778..18389
                     /locus_tag="IV353_001817"
     CDS             17778..18389
                     /locus_tag="IV353_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9010060.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18571..19086
                     /locus_tag="IV353_001818"
     CDS             18571..19086
                     /locus_tag="IV353_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAP9010061.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(19221..>19398)
                     /locus_tag="IV353_001819"
     CDS             complement(19221..>19398)
                     /locus_tag="IV353_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9010062.1"
                     /translation="KTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRA
                     QRRFEGTLRYSLGY"
BASE COUNT         6253 a         2725 c         4220 g         6200 t
ORIGIN      
        1 aaacagaact aatgaaactg aataaaaata tagagtgaac tagtttctaa gagggatttt
       61 aaactagatt tcattggtaa taaaatttgg acagatggaa aaacatcaca taaggaagca
      121 gattttgttg tcatttttgt tcctacaaac tatggtagtg aatgtgttat tgaattagtg
      181 atgtctgtta cttcagaggc attggggata attaaatcca ctatacctgt aggatataca
      241 aaaagtggaa gaaaaaaata ttatcaatag attttattta gttcagaatt tttacgtgaa
      301 agtagattat taatgataat ctttatccaa ttagaattat tatcgtagga tgtgatgatg
      361 aaagccgtga aattgttcaa gtatttacca atgtctccct gccagtaaat ccgcttagaa
      421 tttgagcctt gaaaatgtgc aatgataaaa aacaaaaact cgctcaaaat agacagtttg
      481 gggtgggaat agaccaataa tgtaccaaac aaacttccat ttggggtgaa aataggtcaa
      541 taaagcgatg ttaaacatac ctttggagta agaatagagg gtaataagtg aatttataaa
      601 ctttttggtg actggttgag tggaaaatgt aggtacattt caatgcgaaa atgtattata
      661 accccaaacg aggtcaataa aaatgactca aacttgcaat ataaccctaa acgggtaggg
      721 gatttatgaa aaattgaatt tgccggcgta ggagctggct gttttaagta tgaatgaaca
      781 cagctttcgg gctgttttat ataaatagca ggttactgtt cacaaacagc attttgttaa
      841 gaaagtagtg accacagcag tggtggaaag aggaaatatt atgaagaaac agtataacat
      901 tgcagtcgcc ggcaccggtt atgttggttt gagtattgcc actctgttgg cacagcacca
      961 taaggttaca gcggtggata tcattccaga gaaggttgaa ctgattaata accgtaaatc
     1021 tccaatccag gatgattata ttgaaaagta cttggctgag aaagaactga atctgaccgc
     1081 aacattggat gcaaaggcag cataccagtc tgcggatttt gttgttattg cggctcctac
     1141 taactacaat agccgcactc aacattttga cacatccgct gttgaggctg ttattaggct
     1201 ggtcatggaa gtaaatcctg atgccattat ggttattaag tccaccattc ctgttggata
     1261 caccgcttct gttcgtgtaa agactggtag caagaatatt attttcagcc ctgagttcct
     1321 gcgtgaaagt aaggcactgt atgacaatct gtatccttcg cgtattattg tgggtaccga
     1381 tatggatgat gagcgtttgg ttgaggatgc acacactttt gctacactgc tacaggaagg
     1441 cgcaatcaaa gagaatgtgg acacattgtt catgggcttt actgaagcag aagctgttaa
     1501 gctgtttgcc aatacatatc tggctcttcg tgtcagctac ttcaatgagc tggacactta
     1561 tgcagagatg aaaggcttga acactcagca gatcattaat ggagtttgcc ttgatcctcg
     1621 tattggtact cattacaaca acccttcctt tggttacggt ggctattgcc tgccgaagga
     1681 taccaagcag cttctggcca actacgcaga tgttcctgaa aatctgatcg aggcaatcgt
     1741 tgaatctaac cgtactcgta aggactttat tgcggatcgt gtacttcaga tggctggtta
     1801 ttacaattac gcagaagcaa atcagtatga tgctggcaaa gaaaagcctg ttgtgattgg
     1861 tgtttaccgt ttgaccatga aatccaattc tgataacttc cgccagagtt ctatccaggg
     1921 cgttatgaag cgtgtaaaag ctaaaggtgc aactgttgtg atttatgagc cgaccttgaa
     1981 agatggagag actttctttg gcagtaaggt tgtgaatgat ttggaacagt ttaaggcaat
     2041 gtgtgattct gttatagcaa atcgctacga tgcttgcctt gatgacatta aggaaaaagt
     2101 atatacccgt gatatcttcc agagagacta agattaggag agagtattta tgattattac
     2161 aaaaacaccg tttcgtatgt ctttttttgg cggtggaacg gatatggagg attatttcaa
     2221 agagaatggc ggtgcagttc tttctactac ttttgataag tactgttatg tgaatgtgag
     2281 acatctgcct agattttttg attacagcgc tgagttatct tattctcgta ctgagagagt
     2341 aactagcatt gaagatattg agcatcctgc gattcgtaat gcgatgaaaa tgctcgatat
     2401 gcatgaaatt cggcttacat acgaagctga tttacctgct cgctctggtc tgggtacatc
     2461 cagttctttt gcagttggta tgttgaatgc tttttatgca ctggagggta agtatgctga
     2521 caagaagaaa cttgctgatg aagccattta tcttgaaaga gttctgtgta atgaagccgg
     2581 tggatggcag gatcagattg ccgcatctta tggtgggttc aatcgaatta atttcaatgt
     2641 ggatggttat gaggtgctgc ctgtcattat ttcgactgaa cgtaaaaagc agcttaacaa
     2701 caatctgatg atgttcttta ccggattcac tagattttct tctgatgttc aaaaggctaa
     2761 caacgtcagt ggcacagagg ataagagagt aagactgaaa aagatgtatg agcttgtaga
     2821 tgaagctgag gcaattctta ctgataagaa cagaaattta gatgatttcg gtagacagct
     2881 tgatgttact tggagattga aaaaaggtac tggtggagca attagtacag gaagcattga
     2941 tgaactatat gaaaagggta tggctgcagg tgctttaggc ggaaagttgc tcggtgcagg
     3001 tggtggaggt ttcttggttt tctatgtaca acctgagaaa caggatgcag ttcgttgggc
     3061 tatgcgtgat ttgatgtata ttccgtttga atttgaaaat ggtggaacta gagttattca
     3121 ctacacgccg gaaacttata ttgaaaaaca gaatggataa ggagtaacaa tgttagcgga
     3181 aaaacttaat aaacatattg acttattgat taaacgatat cctacacttg agacgattaa
     3241 acaggacatt atcgatggat acctaattat ggaggaatgc tatttgaatg gtggtaaact
     3301 tcttgtggca ggaaacggag gatcagccgc tgactcggaa catattgctg gcgagttgat
     3361 gaagcggttt aagacgccac gtcctgtaag taaggaattt gccgataagt tagttgcaat
     3421 tgaccctgta agaggtccgg aattagccaa aaatctcgag tgtggtttaa cggcaattcc
     3481 tttggtcgca cacgaagcat tgtctactgc ttatattaat gatgtcgatg gtttgggtgt
     3541 cttcgcacag cagctgtttg gctttggtag atctggtgac gtattccttg gtatttcgac
     3601 gtccggaaat agcaagaata ttatgagcgc aactgttgtt gctagggctc tcggtataaa
     3661 agttattggc ttgacgggcg caaagggtgg ggaacttgcc acggtagcag atgtttgtgt
     3721 taaggctcct gaagttgaaa catacatgat tcaagagtat cacttgccga tatatcattg
     3781 ttggtgtttg atgctggaag ataaattctt tggaaacgaa taaaatttgg aggaatgtta
     3841 aatgaaaaag acaattatgg ttaccggtgg ttgcggtatg attggctcca atcttgttaa
     3901 gagactggtg aaggaaggct gctgggatgt ttatgtagct gataatctgt ggagaggaaa
     3961 gttggagtac ctgaacgacg aagatgggca tcctgtcatc gacctggata ctcatttctt
     4021 caatgctgat ctgactgatt atgagcaggc taaaaaggtt attggtacga ctgagtatgt
     4081 agttcatctg gctgatgttg tggctggcat tgactatgtg tttaagaacc agggcgaatt
     4141 gttccgaatc aataatctga tcaattctaa tgtttttgat tgttgccgta aggctggcaa
     4201 ggagaaaatc aagggtgtta tctatgttgg taccgtatgc tccttccctc tgaccagaca
     4261 gaatactttg aatcctgagc ctctccgtga agaggagttg ttccctgctc ttcctgagtc
     4321 tgcttatggc tggagcaaac tgatgggcca gttggagatg gggtatttgg aaaaagaaac
     4381 cggaattccg tgctgtatcc tgatgctcca taatgtttat ggcacaccta ctgattttgg
     4441 cgaacgtagt caggttattc ctgctctgat tcgtaaagca atcaataatc ctgaagagcc
     4501 ttttaatgtg tggggtagcg gagagcaggg acgtgctttc attcatgtca atgatatcgt
     4561 aaatgcattg gtgctcgccc tggataaggg ttggggtcat ggtcatattc agattggccc
     4621 ttctcactgt acttccatca aggaaattgc atataagatc attgaaattt ctggcaaaga
     4681 catcaagcct ttctttgata cttctaagcc tgaaggtgat aaggctcgtt gcgcagatta
     4741 ttctaaggcg aaagagattt tggattggga gcctactgtt tctttggaag agggtcttcg
     4801 tgaatcctac aattggattg aaaagcagat taagggttaa taagtatata agctgttctt
     4861 cctccgtgta attagaggat gcggaggaag aacatatgag taaggatgta ctgagatgga
     4921 aaagaatatg tataaaagaa aacgtgattt cactgtgtgt ggaatagtag gtgtttgtgc
     4981 tactacaaag aacgagtttg taagggaagt tttttccatg ccaacatctc cggtaagtgc
     5041 gactgttgat ctcgtgggag tgccggccat tatttcggca aaggaaaatc ccaaaattgc
     5101 tgaaatgtat agccaggcta caatggctgc aatagatggt atgcctattg ttaaaattgg
     5161 acggcgcaaa ggatttacct gtgaacgatg tgctgcacca gatattatgg gaatggtttt
     5221 tgaagaaggc gttagacagg gaaaaacaca ttatttctat ggtggaaaag ataacgaagt
     5281 gttaaataag cttcgtgcaa acctagaaag agattacccg ggaatcaaaa ttgttggaat
     5341 gtattcccca ccatttcgtc cgttaaatga agaagaggat agcaagattt gcgaggaaat
     5401 caacaactta aatccagatt ttctgtgggt tggtattggt gcgcctaagc aagaaatgtg
     5461 gatgtgggaa caccagaaga agatccataa cacagtaatg ttcggtgtag gcgccggctt
     5521 taactttttt gcgggcactt tggaaaaagc tcctgaatgg atggaaaaag ctagtctaga
     5581 gtggttattt agactggtaa aggaacctcg tagactatgg aggcggtata tcttgggagg
     5641 tataaagtat atctactata ctattgaatc taagtagggg ggcaataaaa atagatgaaa
     5701 atacttatag ttagttacaa tgacgaataa ctttggtaag aaggggtcct ataattctca
     5761 ggaaatagga ttagctcgag ggttagctga attaggccac gaagttattg tatataaaag
     5821 tgtaaaaagt gatgaggaag ttggaacaga gcaattaagc aatacggcga tgattcacta
     5881 tatcaagtgt cgttatgtag gatcaaatgg attcttatca acaaagaatt ttgataagaa
     5941 tgcggatgtt ctaatacaat ttgcggacct tcaactatca gtacctcatg tatactcatg
     6001 gactgtaaaa aatggaataa aatatatacc ttataatggg ataacgcgaa gtcaaagtgg
     6061 caataaatat ataaagacaa ttattgaaat attggcgcat cgaaattata aaatttataa
     6121 gaaaaatggg tgtgttgcca aaaatgatcg agtttctgaa aaattagtac aattaggagt
     6181 aaataaagtg aaacttgctc ctgtttgtgt ggattttgaa cagttacata agaattacga
     6241 agcaatagat tttactactc tcagagaaaa atggaatatt cctcgcaatg ttcgtgttat
     6301 tttatatgta ggacggtttg aaaaggataa aagaccgtta gatatgatta gtttatttaa
     6361 taaacttgat gagcataatt atttgctgat ggtaggaact ggtgctttac tcgagcaaac
     6421 gaagcagcag ataaatttac ttggactgca gaatagagta aaaataattg agcgtattcc
     6481 taacaaagat atttgggagt tatattgctt atcggatgta tttgtaaatc taaactatac
     6541 tgaaatttgg ggtatggttt tgctagaggc aatgtactat cgcctcggga ttattgctgt
     6601 taatgcacct ggcccacaga atattatttt ggataataga tatggaatgt tggctaaatc
     6661 tgacgatgaa attttgacat tcttaaaaga cggacgattt accaacgata tcatagatgc
     6721 agcatatcag cgtgtaacta cagaatttag atggtcaaag tcgggagaaa ttatgcttag
     6781 catgttggaa ggtgataaat gatatggaag taacgaaatt aatggcattg aggaatcgat
     6841 atgctttaaa catagttgat aattgcacga gaaaaatagc caaaattctt ggttgttgta
     6901 ttggtaaagg tgcacagata gggaactctg ttgaatttgt tcataattct gtaggcactg
     6961 tgattcattc tgacacaatc cttgaagatg gtgttaaagt ctatcagaat gtgacgtgtg
     7021 gttgagcaga tagtaatgaa cgtatcatca ttcgagtaaa aaagaacgct gttttatgtg
     7081 tgggtgctaa aattctttgt aaatctaaca cagaattatg cgttggggaa ggtgctgtag
     7141 ttgctgcaaa tacagtggta ttgaggtcta ttccacccta cgaagtgtgg ggggtacctg
     7201 ctaaaaagat ggggagataa aacatgaatg catataaatt atacaaagta gaacgatggt
     7261 tttacttaca caaaatggag ttttttgcca agattgtaaa ggcactaatt tatgtacttc
     7321 ataatagctc aatttcatat caaacagaaa ttggagataa ttgtaagttc ttatacggtg
     7381 gcataggttg tgtaattgga aaagatacaa aaataggtaa tcacgttata atcggaacga
     7441 atgtactgac tggtggtaga tccaataagc ctggtatgcc tacaattgga aacaatgtat
     7501 atatcgctac tggcgcaaaa attttaggta atatcacaat aggggacaat gtaatagtag
     7561 gggctaatgc tgtggtcatt aatgatgttg aacctaattg ttcggtagga ggcgttccgg
     7621 ctagagtatt gaaaaaagat attgatgtaa atgattattg tagtttgaat cgttactaaa
     7681 aggagagatt gtatgttgta ttttgttatt cctttaatga gtgcgaaggc agcaaacaat
     7741 tgggagatgg ttagtacctt atttaaccga acattatggt cgtgttataa tcagactgat
     7801 cctgatttca aaatcattgt tgcttgccat gaaattccta aactcacgca tgaatatgat
     7861 gacagagttg aatttattca agttagcgaa aaggatgccc ctattcccac aaatcaagac
     7921 gaaaaaatga ttgataaggg ttataaaaca catgctctcg caatgcgttt gcgagagttg
     7981 ggcggcggtt tcgctatgat ggttgacgca gatgatcttg taagttctca tttggcggaa
     8041 tatgtgaatt cccatcccca ggaaaatgga ttttatgtca aaacgggata cgtgtacttt
     8101 gttggagacg attatatgaa ggttctacca aagttctcat caggaagtgc gtgtatagta
     8161 aaatatgcag tggaagacct gcctgaggca tatccggaag ttatgacagc aaattgtgat
     8221 gataatccat ggatcattag aaaacgacat gggggcgttg ttccagcatg tgaagagcaa
     8281 gggcgtccat taaaacctct tccattcaaa gcagcagtat atgtattagg aacaggggac
     8341 aatcactcgc tttatggtaa aacgacaaaa tatcagacga aaatgcgaga gatactggaa
     8401 atgtttatag caaaacgtaa aattaaagga aaactgaaag cagaattttc tgttgattgg
     8461 ttataaggtg agtattatga aggtattgat gtgtgaaagc caaatgccag agaattggta
     8521 ttcgtttgat ctttctaaag aacttaatca atatgttgat ttaacattgc tgtgtaagcg
     8581 tgatgttcct cctttagatc ctaaaattaa gagcaaacat attttgtatc ataaaagtac
     8641 aaataaagtg ttttcggcac ttgtttttct gaaaagcttg ttcttaattt ggaaggaaat
     8701 aagatttggt gattatgata ttgttcattt gcaattgatg aaaagtctca aatatgaagg
     8761 cggattgtac gttttatgta agagacacta taaaaaatta attatgacag ttcataatgt
     8821 gttaccacat gaggttcgtg aaaaggatcg ccaagagcac tttaatatat attcaaattg
     8881 tgatggtttc attgttcaca atcgagtaac agaacagcat ttatatgctg aattcccaga
     8941 attaaatgca aagtcatatg tttgtccaca tgctatctat ggagatgtta aacatatatc
     9001 tagagcgtat gataaagata agtttgaatt tatcttgttt ggacaaattc gtccatataa
     9061 gggaattgat gtactgctta atgcaatcac agaacttcca atggatgtaa gaaagaaagt
     9121 tcattttacc atagcaggtc aacaacatcc taaacaggat agtactgact ataaaaaaat
     9181 gattgaagat ttaaaggttt cagattgtgt tctatataag ccagagagaa tttctgatga
     9241 agaaatggac agtatgttca gaaatgcaga tgccgcaata tttccatata aagaaatcta
     9301 tggaagtgga gcgcttctta tggcatacac ttattgctta ccaactattg ttagtaatgt
     9361 acctgctttt atggaagaaa ccgaggaagg aaagataggg ttacttttcg aaaatttgaa
     9421 ttctaagagc ctagcagaca caattgtaaa gtatgtgaac cttagcccag ctgagaaaag
     9481 caaaatagag gatgatatat atgagcttgt aaaccgcaag tatacctggg cggcatctgc
     9541 aaaactcaca tacgaggcat acaaacaaat tttgaatgag tagtaaaaca attagaaagg
     9601 aataataatt aacatgcaga ttagtaaatg gaaaatcata cagtttctaa tggtctttct
     9661 aatgattatc tcatcaagca tattgaagcc tttgggcggc tggttgggag aaacgatgaa
     9721 cgttagactt gtgacagtca ttctagcaat agtatgtttg ttaatagcct cattaccagc
     9781 atttagaaaa aatagatatt taaatttctg cactttactg atttgtgttg ttataatagc
     9841 agcttacatc tactcagata agacaatatg ggttaaatct agggaaagta tgagtttata
     9901 ctatgtatta ttggcgtatc cattgttctg tactttaatt aaccaagcgt ggaatttgga
     9961 ttcaatgcta aatacgatat gtggtgtaac atttctttca tacattctta gaacatctat
    10021 ttcacttatt cagaaatttt caggagtact tctctatgaa aacatatatc gtgagtgtgc
    10081 cccggaaaat tggtatcgtg gaggcttttt aagaattaat ccaccatgtt ttacaattat
    10141 aataataccg attgctttgt atatgattga gcgacaagtt gtagctagaa gaaaagtaaa
    10201 atattattta ctgattgcta gcgcactggc atattctgct gttattcatg gttctagatc
    10261 tgtccttgtc tatcaaatta ttgtacttgg ttgctatatc ctttttaaga aaggttcttc
    10321 gaaaagaaaa attgcaatgt gggttttagc tgggttgctt gtagtcatat ttattaattc
    10381 gaccatgttc tcaacatttg ttgaaacttt cacaaacagt acaggtgaat atgcaggatc
    10441 tgctgaatct cgatttgctt cagtttggtt ttttattcct aaatttttgc aatcgccttt
    10501 gttcggtact ggtattctta ctggtgagga atttacgttt atattaccag gaggagttag
    10561 aggacaccta tctgatattg gatttttcta tacaattact caatatggtg taggtatact
    10621 tatatttgat attctgttta ttataaaagg gttcaaaaca gcattacttg catcaaaagt
    10681 gggtatgact ggctatcaat atttagcatt tggaattaca ttaaatgtct tgctaacagg
    10741 gattaatata gactggtttt atccgatatt tactccagct attcctgtat gcttagcggt
    10801 tatagaatac atctatcaag aatgtagaag tgtggctaac atagaatagg aaatattaat
    10861 aggagtgcat ctcatgaata agaaagagat tttatataac atattgaaga agtacgaaaa
    10921 tttaccatat gtgtttagga acatgtttgg tcataatata agtaaactcg tatcttggaa
    10981 attgactgct gattgtgcaa cagtagaaga aaattctgaa gtttatgcag cgtttcaatt
    11041 gcgctcaaag gacatgtctg atgttccagt gatgggttta gttaattgtt gcaaagatgt
    11101 agcaattgta atgcaggggc cactatgttt aaaggatgat ttctcttata atactctggc
    11161 ctactataag aaatgttatc caaatgcttt aattattctt tcgacatgga aggacgaaga
    11221 tattaaggca ttgaaaagga tagaggatct tggagttata gttatttgta gtgagaaacc
    11281 tgaaaagcct ggacacttaa atattaatta tcaagttgga aacactttgc ctggaatagt
    11341 cagagcaaag cagcttggtg caaagtatgt ttgtaaaact agaacagatc aaagaatata
    11401 ccatccaaat gcaatggcgt ttttctgcag tttacttgaa cagtatcctg ttaataacga
    11461 agatgattgg aaattaatac aaaatcagcg attactgtta ctatcaatgc cgtatggaga
    11521 catgtttttc ccgtattgtt tgtcagactt cctgtatttt ggtgatgttg atgatatgat
    11581 aaacctcttc tcgattccta gtgatgtgag agaaaaggga gctgtgtcta gaggagtctc
    11641 aagaagggag atatctgaaa ataatttagc tccagaagtt cagcttcttc gaagctatat
    11701 ttcacgaatg gggggcaatg aggagtgctc tattagggca tattgggagt ttgtaaaaaa
    11761 ccatgttatt actattaaca aaaatcaaat tgatttgtac tggcataaat atgtaggtcg
    11821 ttattcaaat aatactattt atggtacgta ttatattgat gatagccaag atgcacttca
    11881 ctgctataat tttgatttta taaattggtt gaatttgtac actggaaaat acgaatacaa
    11941 agcagaatat gaaaaatata tggactttgt ttgggaagag taattaactt ttatagtaaa
    12001 aagtacttta gggaagggca tattatgaaa acagtaatta tggcgggcgg acgaggaagc
    12061 cgaatttcgg aattgtttcc ggatattcct aagccaatga ttcctataga tggcattcca
    12121 gttttagaac gagaattgtg ttcgcttaga gatcaaggct ttaatgatat tattttgact
    12181 gtttcctatt tgcatgaaaa aatagaggag catttcggag atggttcaaa attaggaatt
    12241 aaaatcaaat attttattga aaaaactcca cttggcaatg caggtgcatt gtattttttg
    12301 ttggatgaac tgggtgatga accgttttta cttttgaatg ctgatgcagt atttgatgtt
    12361 gatttcaggc agatgattag atttcatgtt gagaataacg cagatgttac tttgtttacg
    12421 catcctaact cacatccgta tgatagtgga ttaattatat ccgatgacaa taagaaagtg
    12481 cttcaatggt tagcgaagga agatcaaaga cctgaatatt ataaaaaccg agtaaatgca
    12541 ggtcttcatg ttataaatcc gaaggtgttg agagaaaatc cattaatgga aattgtaaat
    12601 aaatcaatag ttgaaggtgg ggggctacca tacaaaattg atttagatag aatgattctg
    12661 aaaccattgg ctaacacagg gcgtatgtat tgctacgatt ctccagagta tgttaaagat
    12721 atgggaacac cggaccgata tagagctgta attaaggact ttcaaaaggg aattattgtt
    12781 ggtaaaaatc tatccaatcc acaaaaagca atctttttag atagagatgg aacgattaat
    12841 aaatacgttg gttttttgag aaatgcagaa gaatttgagt taattgatgg agtatcggag
    12901 gcaattaaaa ttattaattc tctaggtttt ctgtgtattg ttataacaaa tcagcctgta
    12961 attgcaagag gtgaagttac tgttgatgag cttaatagta ttcacaataa gcttgaaaca
    13021 atgctagggc atgctggtgg atttttaaac ggtatttatt actgtccgca ccatccccat
    13081 aaaggatatg caggagaggt tatagaatta aaaattgatt gcaattgtcg caagccaaaa
    13141 cctggactgt tattagaagc atcaaaggat tttaacataa acctaagtca atcatttatg
    13201 gttggagata gtgaaagtga tgtaattgct ggaaaaaatg ctggttgtaa gacagtactg
    13261 attacagaag acatcacctt gcctgctaat tatggccaag actatagtgt ctgttcattg
    13321 ttagaatttg ttaaaaatca tttatgcact atgcaataat tggtttagtg aaacttgtaa
    13381 aaggagtaat tagatgaagg tattaattcc agcagcaaaa gttgttgaaa aagaattgca
    13441 gagtatagga atgttaccag caattatcta tccaattaat aaaaaaattg tctttgatta
    13501 tttgtataat cagtataatg caatctgcga ttcattggat attatctgtt atgaaaaagc
    13561 ggataaggtt catagaagac tgaaaaacta tcatgattcc agaattcata tatgtgacct
    13621 tcctgaatta ggggatctag ggcaaaccgt gtattttggg ttatcagatg aagatgagcc
    13681 tgttctaatt aacttcgcag atacaattgt tatggagagt atgtctgata tagaaatgga
    13741 tacattctat tattctgaag attttgtgtc gagtacttgg acatacttta aaattaatga
    13801 aggaataatt actgatatcc acgataaaga ggaagcttac gtatccgata aacgtgaaaa
    13861 attatttatt ggcgtattta tgttttcttc gtcaaaactt ctgaagagtt gcttaagaga
    13921 ggcagttgag agtcggaaac aggggggaat aaactcattt tatcgggctt tacagctata
    13981 cagcgaacgt tgtccgctaa aggccgtaag aacagaaaag tggtttgata ttggacacat
    14041 cgataaatat tacaatacaa agttggaagt taaagcacga gagtttaatc atatctctat
    14101 agataaagat cgagggatat tgaagaaaac tagcgatgac aaggataaat ttattggaga
    14161 aataaagtgg tatttaaaac ttcctgctga tattgaatat gtaagaccta gaatttttga
    14221 ttattctaca tcttacgtag ctccctatgt tgatatggaa tattatgcat atcatacggt
    14281 tcatgagtta tacttatatg gtgatttaaa atataaccag tggcatgatc tgtttagccg
    14341 tataaagttt atctgtagcg attttaagcg atacacctta aaagatgaaa agattagaga
    14401 gtcattggaa gatatgtatt tgcgaaaaac catgcagaga tttcaaaaat tgtctgaaga
    14461 taaaagattt tcagcttttt ttgagaaaca aattcaaatt aatggtcgta agtttatttc
    14521 tttaaccggt gtaattgaac ttctaaagaa tcttatccca agtatgctct atgatgtaaa
    14581 tgaattttgt attatacatg gagatttgtg ttttgctaat atgatggtag attccaattg
    14641 ttcctttgtt aaggttattg atcctagggg aaaatttgga aaatatgata tttatgggga
    14701 cagcagatat gaattagcaa aactatttca tagtttagat ggtaaatatg attatatcat
    14761 caaggatcta tttactgtag aatatgatac cgagtgtgca gtgatagatt atacaattaa
    14821 tacgcgtgta agagattacg atttatttga attgtttaag tcggtctttt cggacgagat
    14881 taaggacgat tttgataaga ttgtattgat agaagcgttg ctatttctta gtatgattcc
    14941 attacacggt gaaagttttg atcaccaaat agtgatgctt gctacaggta tggaacttct
    15001 tgcaagagta agtaataaag ttatactcta atataaattg tgccttaatg aataacggtt
    15061 acaatactgt tttagcaatg taggcattaa ggcacgatta tattaattaa tacaatagaa
    15121 gggaagagaa aaatgtatag tgaatattct tttgtgtttg atatcgatgg cactttgtgt
    15181 ccgataaaga agaaggatga gcgatatgag gatttggtgc cgtatgagaa tatggttgaa
    15241 aaactgagat tctataagga gaatggtgct aaaattacat tgttcacctc acgcaatgtt
    15301 aattcctata aaggaaattt gggaatgata aatgctaata ctgcaaaagt gctcttggcg
    15361 tggttggata aatgggacat tccgtatgat gaaatcctgt atggtaagcc ttggccgggg
    15421 cataaaggct tctatgtaga tgatcgaacg gttagacctg atgagttttt gagatgctct
    15481 gtagatgaat taaatgaaat ctgcaacagt tcaaaaaaaa gattctaaat aaggaggtta
    15541 gttctatgga aaaaaagatg acagttatca ttacgatggc aggtttggga agtcgttttc
    15601 gaaaaattgg ttatgcggtt cctaaatata tgattaatgc caaaggaaga actttatttg
    15661 attgggccat ggatagtttg gttggataca ataaaaatgt tgataagtat atttttgttg
    15721 ttcgtcagga agacaacgct tcagctttta taaaggagca ttgtgcttac tttggtattg
    15781 aaaacgtaga agtgattgaa atagactata tgacagatgg tcaagcaact acatgtatgt
    15841 tggctattcc ttattgcaat caagacaatg agattatggt ttacaatatt gatacatacg
    15901 tggaatcaaa tgagatgaaa tacgaagata tttgtggaga tggacatatt ccatgctttc
    15961 atgctgatgg cgatcattgg agttttgcaa agcttaacga tcgaaatgaa gtaatagaag
    16021 tccgcgaaaa agttcgcatt tctgataatt gtactcttgg tgcgtattat ttctcatcgg
    16081 caaagctgta taaagcttta tatgaagagt attatgcaga ctcctctaat atggagaaga
    16141 atgaaaaata tatagcacca ttatataact ttatgataaa aaaagggtta aaggtgacta
    16201 ttagcatagt agatgcgaaa aaggttcatg tgcttggtac tccagaagag ctcaatgtgt
    16261 ttttgaatca agaataaata gtgtttagga gaaaatagca gtgaccagta ataatctaat
    16321 tgtaaaaaca ttaagcagat ataagaatac tccacttcct gttaaggctg cattatggtt
    16381 tatggtatgt ggttttttac agagaggggt ttcaatgatt acaaccccaa tttttactag
    16441 agttttgtct acagaacaat acggtttata cagcacttat ctatcgtggg aatccttatt
    16501 gctcatgatt acatcgctta gtttgttcaa agccatgatg aatctttatg taaaatatga
    16561 taatcaagaa catattcttt cgacagtatc cggattgact ttagtactga ctattttttg
    16621 gtttgttatt gctattttta ttaaggatat attggcagaa attttaggat tgacaccagt
    16681 tctagttgtc tgtatgttct catattttat atttcaggca gaaatacaat gttggtcttt
    16741 acacaaacga tatatatatg attataagac tttattagta gctacccttg cagttactgt
    16801 tggctcatct gtagttggct gtttagcggt tctgtttata tctcctaccg ctgaggcgag
    16861 agccttaatg aatgtcctag tgactggtgt tgttggcgtg gctgtgtata tatcggtatt
    16921 tagaagatgt aaggatttta aggataatga agtgtggaat ttcgcagtag tattttgtgt
    16981 atcgttgatg ccacactatt tatctgaatt tattctgaat ggttctgata aaattatgat
    17041 aaattactta tgtggttcgt cagatgttgc tatttacagc gtggcatatt ctattggtgc
    17101 tctaattaac cttgttacaa attctgtcaa ttccactttt gcaccttatc agtatcaaaa
    17161 aatcaagtca ggagaatatg atttactatc aaaaagagct aatgaagttc ttggtttggt
    17221 agcggctatg ctttttgcat taatgctatt tagtaaagaa attgtgttga tatttggtgg
    17281 gcagaaatat atcgacagtg ttaatttaat tgtacctata tgtatcggta tttatttcaa
    17341 ctatatgttt cagctgtttg ctagagtaca ggaatacttt gaaaagaaac tcactgtcgt
    17401 tattccatcg gttttgtgtg caattatgaa tattgtacta aattatgttt ttataaagtt
    17461 atatgggtat caggcggtag catacacaac tgtattctca tatatgtttt tctgcgtcat
    17521 ccattatatt ttttatagaa aagtttgcaa agaactattg gatggaaaaa acatatatga
    17581 tgtcagagga atacttttga tttctgcaat cacgatagct ggtggcatcc taatttcaat
    17641 aattaataaa tttgatttca taaaatattt aattatagca attgcttgtg tcgctgcaat
    17701 tatatttaga aaacgattgc tacttttagt aaggagaatt atgagcaaat aaacatttgc
    17761 tcatagagag atagagaatg atagatatta tttctgctat aacggtaaat gtattaacag
    17821 cattatatca gccgttctta tttgccgtat taacagcgta tctgtttatg tactggtatc
    17881 tatttatagt tgataactct cgagatccta aaaacttaaa agattctgta ttaatctggt
    17941 tgagatattt taaagcatct agtaaatttc gcagactctt ttttcttgcg ttttatttta
    18001 ctttgatatt atttaaaacc ttgcttaatc gaaatatgtg gatgaacccg ctttcaaacg
    18061 ttattggaca gtggtcatta tatattatag ataatgctac aggagagagc agattgacaa
    18121 cagaatgcgt ggagaacacg atgctgttta tccctttttg catgttattg ttatggtggc
    18181 gtgacattaa atgtgatatt atacgcacca tctatgttgg gattaagtat gtgtttttat
    18241 tctcattaag tatagaattt acacaactgt tttttaggct tggtacattt caactatcgg
    18301 atttgtttta taacacccta gggggcttaa ttggtgcttt gctatattgg ctattctacc
    18361 gattaaataa gtatatagac ttaaaataga actgctttga attttaatat ggttaatatt
    18421 tttctgcaga caggattgtg taatctcatc aagaatactt tacatgccat ctatttttgc
    18481 taaaacattg ttgttatatc ttcaagaggt agggaatttt attgctttaa atgaggtcga
    18541 gcgaattaaa aaattggtaa aatatataac atggtggtag ttatggtaaa agtgtttgat
    18601 ataattaagt tttcggattt cagaggatat actgctgttg cattggataa tgagactctt
    18661 ttctcgattg gtattgagtt tcacattaag caaataaatc aaggatttag tcataataaa
    18721 tatactatgc gagggctaca ttatcagagt ggagagcatg ctcaaggaaa gatagtgatg
    18781 tgcctacatg gtgaaattat gaatatcgca cttgatattc gacaaggatc ttccacttat
    18841 ggatgtgtgt atgtcaacac attgagtgaa agcaatggta aatgtgcgta tatcccacgt
    18901 ggatatgctc atggctatct tactatggag gacaatacgc tagttcagtg gtgcgtagat
    18961 aatgatttca attctaatgc tgccaaatgt ataagttatg aaagcgtaga tattgaatgg
    19021 ccggtcagtc ggcagtgttt cgttataagt gagaaagaca aaaatgtgga aagattaagc
    19081 ttttgattaa aatgagctgt ggagaaaaga aggatactgg aaaataagaa gaacaataac
    19141 cgagcaaatg ctcggttatt tctatgagga atacataaaa ctaaatttag atatagatat
    19201 acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc ttcaaaacgt
    19261 cgttgtgctc gacttaatag aacactgtaa gccagtaata tgctttcaaa tctactctct
    19321 agccctttaa cagaacgtga gttgaaattt tgacaaccta atttttctaa ggaagaaaaa
    19381 acagtctcaa ctgttttt
//
LOCUS       DACDLI010000049        17841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDLI010000049 DACDLI010000000
VERSION     DACDLI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.049"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IV353_001820"
     CDS             4..816
                     /locus_tag="IV353_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9010063.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IV353_001821"
     CDS             813..1613
                     /locus_tag="IV353_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9010064.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IV353_001822"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IV353_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9010065.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IV353_001823"
     CDS             3110..4819
                     /locus_tag="IV353_001823"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9010066.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IV353_001824"
     CDS             5031..9383
                     /locus_tag="IV353_001824"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9010067.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IV353_001825"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IV353_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9010068.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IV353_001826"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IV353_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9010069.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IV353_001827"
     CDS             11221..11514
                     /locus_tag="IV353_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9010070.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IV353_001828"
     CDS             11511..11822
                     /locus_tag="IV353_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9010071.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IV353_001829"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IV353_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9010072.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IV353_001830"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IV353_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9010073.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IV353_001831"
     CDS             complement(14623..14844)
                     /locus_tag="IV353_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9010074.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="IV353_001832"
     CDS             15292..17655
                     /locus_tag="IV353_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP9010075.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            complement(17711..>17841)
                     /locus_tag="IV353_001833"
     CDS             complement(17711..>17841)
                     /locus_tag="IV353_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9010076.1"
                     /translation="QVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLT"
BASE COUNT         6073 a         3164 c         3929 g         4675 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccaaaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt
    17701 atttgtcaaa ttaagtgaga caaaactttc catttacgta actcaaaaaa acttctaatg
    17761 gtgtttggta atttagtgat tttctaggga tatggtttct tttggacgca acggatgaga
    17821 tgaatccttg attgacttgg t
//
LOCUS       DACDLI010000050        17733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDLI010000050 DACDLI010000000
VERSION     DACDLI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.050"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IV353_001834"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IV353_001834"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9010077.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="IV353_001835"
     CDS             complement(2262..2513)
                     /locus_tag="IV353_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9010078.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="IV353_001836"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="IV353_001836"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9010079.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="IV353_001837"
     CDS             3463..3681
                     /locus_tag="IV353_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010080.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="IV353_001838"
     CDS             complement(3970..4482)
                     /locus_tag="IV353_001838"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9010081.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IV353_001839"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IV353_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9010082.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7369)
                     /locus_tag="IV353_001840"
     CDS             complement(6935..7369)
                     /locus_tag="IV353_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9010083.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="IV353_001841"
     CDS             complement(7379..8167)
                     /locus_tag="IV353_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9010084.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="IV353_001842"
     CDS             complement(8164..9105)
                     /locus_tag="IV353_001842"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9010085.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="IV353_001843"
     CDS             complement(9282..9869)
                     /locus_tag="IV353_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9010086.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IV353_001844"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IV353_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9010087.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11700..12710
                     /gene="gap"
                     /locus_tag="IV353_001845"
     CDS             11700..12710
                     /gene="gap"
                     /locus_tag="IV353_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9010088.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12795..13730)
                     /locus_tag="IV353_001846"
     CDS             complement(12795..13730)
                     /locus_tag="IV353_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9010089.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13916..14875
                     /locus_tag="IV353_001847"
     CDS             13916..14875
                     /locus_tag="IV353_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9010090.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14961..16055)
                     /locus_tag="IV353_001848"
     CDS             complement(14961..16055)
                     /locus_tag="IV353_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9010091.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16244..17005
                     /locus_tag="IV353_001849"
     CDS             16244..17005
                     /locus_tag="IV353_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9010092.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17120..17560)
                     /gene="fabZ"
                     /locus_tag="IV353_001850"
     CDS             complement(17120..17560)
                     /gene="fabZ"
                     /locus_tag="IV353_001850"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9010093.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         5108 a         3848 c         3097 g         5680 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgta aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgtatta attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata
    11701 tgacagtaaa agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca
    11761 tcaaagaagt ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta
    11821 tgttagcaca attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg
    11881 caacagaaga ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg
    11941 caagcaaaat cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt
    12001 tttatacatc tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta
    12061 tttctgcccc agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag
    12121 atgaaaacga caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg
    12181 cttatttctt aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct
    12241 acacttccac acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc
    12301 gttcagctgc tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag
    12361 ttatccctga gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg
    12421 gttcattaac tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg
    12481 aagcaatcaa aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg
    12541 tttctagcga tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag
    12601 taacaactgc tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg
    12661 gatttacttg ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat
    12721 caatcgataa aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct
    12781 acttttaaac tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat
    12841 ttttcaggat ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct
    12901 tttggtccct tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct
    12961 tcgtctccat gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt
    13021 gcttcaccga aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc
    13081 ggaaatgctg gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa
    13141 gaataaccgc aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc
    13201 atcataactg ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca
    13261 ttgttttcta gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat
    13321 ccaatgtaat ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa
    13381 cccatatcat gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca
    13441 tgagccacaa gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat
    13501 ttcagaccgt cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat
    13561 ttatgtgcat ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag
    13621 tctttctcag aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca
    13681 acaatcagag ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca
    13741 tctcatttct cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc
    13801 aatgttcatt tttataaccc gcgaattgta aaattaagtt agaaaaataa aaaggcattt
    13861 atggtacact caaattgtat ttccgacgaa agaaaacaaa ggagtgtaac cataaatgac
    13921 ctacaaacat cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa
    13981 taagccggtt gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt
    14041 caataagttc aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa
    14101 gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga
    14161 gaaagtcact cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggcctgtttc
    14221 atgcggtatg agaacacttt atcgtttatt ttctaaagga atatttgata ttgacacact
    14281 accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta
    14341 tcagcgctca atccatgatc gacctgataa ttatcctgat ttcaattctg agtttggtca
    14401 ccttgaaggt gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga
    14461 aagattatct aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga
    14521 aactgccctt aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt
    14581 tgactgtgga aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat
    14641 atattttgcg gaccctggaa caccttctca acgcccatta aacgagaatt ctaacgggat
    14701 tctgcgtcgt aatggactgc cgaaatcaat ggattttaga aaagtgaatc agacatttat
    14761 ttccagtgtc agcaatcaac gtaatcatat tccaagaaaa tcattgaatt acagaacacc
    14821 aatcgaaata tttttgagct atgtacaaga agcattttat tctagcttaa tttgacaaat
    14881 cataacctat aaaaaaacaa aataaaaaag cagaaataac tggctatttc tagcggatta
    14941 tttctgcaat aactaattat ctatttttta ttttcaatta atttaaagct tgaccatggt
    15001 ttcaaaaagt caaggaggaa taattcgtca tcggagattc ttcctacaac attgacacgt
    15061 ccgtcgtttt tcatatcctt caaggcgatt tgtgtttctc ctttgtattg accgtaccct
    15121 tcattgtcaa tcagtacatc cccatgatgc atatctcgcg tattatgagg cgggaaatct
    15181 ttatctttgt agtatacacg agtcatcgtt gaacgaagga tatattctga gcgatcccct
    15241 cgataactat gaaggttatc aaacaagcag attcgttcgt tttctgtaat accttcttcc
    15301 gtatctactt tcaatgttgg ataatccgcg ttaaatgctt ctgccatttc tttcaattct
    15361 tcttcacttg cgtacgcatt tccaatagaa atatcatcaa tcaatcctgt aaggattaaa
    15421 tgcttcactt gtgtcgcaat ttcaagatct cggtggtctt ccagcgtgca caagccatct
    15481 tgcgtaggcc aagggccaaa ctcagcagat tgcgaattga cgaatgccat cgtattcaaa
    15541 ttatattttc tgaatttttc tgaacaaaaa acaaagtgtt cgtatcctaa accagagtat
    15601 cgatgaggat aaaaattatg agaacctaat aaattatccg tgtttggcga ataactcatg
    15661 atgttgtcaa cgtaattcgt gcccgaactc atattgatct cgattttgat accaaaagga
    15721 ttgcgcgtca tttttgcttc ttctgctcct gtgaatccga tatctaagcg gaccccgtag
    15781 gcacccatct tatggaaaaa agataagtca tcgtaagaaa tctccaactg ttcaaataac
    15841 gctggattga tatcaaccat tacttccatg cctaggctat tcgcgtaatc aactacttct
    15901 ttaaattctg aaagaacttt ttctttatca tcattgatct gcagcaaact ggtaaaaacc
    15961 cgtttatacc catacttatg agctaaatcc aagtacgctt tgtccttttc aaatgttgac
    16021 cgttctggat aaatcgagat tcctaatttt cccatattac catttcctcc tgcatctttt
    16081 cgtttttgta tccacttaca ttatagcaaa cacaaagtaa atggtctaca ccttttcttt
    16141 ttcttttaaa ataaatgata aattccaata atttaagtag gtattttccc tctattttgt
    16201 tatcattaaa gataacaaaa tagtaaattg gaaaggaaca gttatgaaga aaaataaagt
    16261 tattttaagt attagtttag cagcagcatt gctgtttgct gctggttgtt ctgcaaacaa
    16321 cacgacttct tctagttcgt ctacagaaag ttcaaaaagt acatctactg cggaaagcac
    16381 gactgccagt agcgtcgatt tgaacgcatt ggaattacca caattgacga aagatgtcgc
    16441 cgaaaatgaa gatctggtcg aaatggtgac gaataaaggc acaatcaaaa tcaaattatt
    16501 tcccgaacac gcaccaaaag cggttgaaaa ctttatgaca catgcaaaag acggttacta
    16561 caacggactg actttccatc gcgtcatcaa agatttcatg atccaaggtg gcgatcctaa
    16621 aggcgatggc actggagggg aaagcatttg gggagaagga tttgaaacag agatcaacaa
    16681 ccacctttat aatataagag gtgcgttatc tatggctcgc tcacaagatc ctaacagtaa
    16741 cggcagccag ttctttatcg ttcaaaatac agatgatatg catgatggtc ttttaaaaga
    16801 cgactatcct caagcaatca tcgacgctta taaaaacggc ggttatcctt cactagatgg
    16861 ccattatact gtcttcggcc aagtgattga aggaatggat gtcgtggatg ctattgcaaa
    16921 tatcgaaaca gatagttcag acaaaccaaa agaagaagta aaaatcgata aaatcaatat
    16981 actgcaagaa gcaaaacaaa aataaaaaaa taacgtctga atcctgcata gatgacaacg
    17041 tatgctagga ttcaggcgtc ttctttttac ataaaaagca agtaagcttc cttttgtttt
    17101 gctatatcag gttttatagt caagcatttt ggttgacgat gaacgtgaac tcgcactcac
    17161 agactttttt atcatctaca tatgctgcag ataacgctgt accgactgat cccttcattt
    17221 tggtgatttc aaaatgacat ttcagcacat ctcctggaac aactttttga cggaatttgg
    17281 ccttattgat cccaccgata taagctcttt ttcctagaaa ctcttctgat tttaggataa
    17341 gtatcgaacc tgcttgtgcc agtgtttcta gaatcaacgc acccggcatc actggattgt
    17401 caggaaagtg accttggaaa aaatcttcat tgattgttac attttttgtc gcaacgatct
    17461 tttttcctgg ttctaaagta tctacataat caatataaca aataggataa cgattaggaa
    17521 tcaattccat tacttcagta gcagataata cgtaagacat aattaaactc cttctttcag
    17581 gcaattgcca ttttcattac tttgactatc aaagtatcat attcctaaat atttagcaat
    17641 ctttttttaa tttaagaaaa aatagtttga caatcaaaac attttgacta ataattaaga
    17701 aatcaagaag ttattttagc tattattctc tat
//
LOCUS       DACDLI010000051        17725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDLI010000051 DACDLI010000000
VERSION     DACDLI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.051"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..188)
                     /locus_tag="IV353_001851"
     CDS             complement(<1..188)
                     /locus_tag="IV353_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010094.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
     gene            complement(329..664)
                     /locus_tag="IV353_001852"
     CDS             complement(329..664)
                     /locus_tag="IV353_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010095.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFRIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            complement(676..2268)
                     /locus_tag="IV353_001853"
     CDS             complement(676..2268)
                     /locus_tag="IV353_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010096.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIEGVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(2288..2710)
                     /locus_tag="IV353_001854"
     CDS             complement(2288..2710)
                     /locus_tag="IV353_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010097.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            complement(2728..3015)
                     /locus_tag="IV353_001855"
     CDS             complement(2728..3015)
                     /locus_tag="IV353_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010098.1"
                     /translation="MEIKEIKVRGINKKYVQEIDRRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILITH"
     gene            complement(3237..4634)
                     /locus_tag="IV353_001856"
     CDS             complement(3237..4634)
                     /locus_tag="IV353_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010099.1"
                     /translation="MSSINLNHLFEDISNHFQQFSIEDNDELMGLFQIEKEYDYLTFI
                     CREGRIIDPKESKIISHLRRFKNYISKNKETISEIKFDGTTNLEYLVRVTYQRNRRLI
                     EDDQGVLICAGEEYDSLKFTQLKFESIGRSIYFESRPFYIRYNDFQHLFLQDEPNELT
                     ILERRGKKQITLSYERPLTFMDGSCESIFQKINPFIKEMMKEKDSLPFTWVEIIEAKN
                     KYELLCKKYPSKKFTKKVNKYPLRHTYALIKLKPRVTRKQFRRIEAAIEQKDNDLFPS
                     EIFMCARKNSRKFVKDLLVSYVQNCILSNFATIILSDLMDMSFYLNQKLEFNFKTERG
                     LLRQHNQIVEEYNLKQAKRLKTFKLKQHGKYRLLVKHLQNHPHFNLIKTNKELFMEGS
                     TMHHCVYNYLEKIQRGGSTIWKYERQKHRYTVEIEKRKYGNYEVVQCYGKYDSLPDKQ
                     ELDEIKKVVRAIPYK"
     gene            complement(4631..5503)
                     /locus_tag="IV353_001857"
     CDS             complement(4631..5503)
                     /locus_tag="IV353_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010100.1"
                     /translation="MKSSDWMEHTWLPLKRNSKRKEFWILQLWPLGKLCSYLARFILD
                     LIIYSIVVFGTAYYLLNDPNRIAASDIFTVLMTLIFVMLFTIFTQIAMQFTRQKLTAG
                     KLFKSLFDLIIVNAILATLIKQIEQFGRTTKGLKEFLMTFVHFVNGYMYLFEVGIILF
                     LFGYISTKLIKRSIEKEIEIDLIPTLFGYEYYVDFGRYDELINTGIVHTSIYEATVKG
                     IRTTLKDGQESKAIDRRKEVYNFYEVEASLRYGIGKLSLDGRNIPQNKPYIGWKSDAL
                     EVSKKLSMEEDELQ"
     gene            complement(5510..5749)
                     /locus_tag="IV353_001858"
                     /pseudo
     CDS             complement(5510..5749)
                     /locus_tag="IV353_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5767..5997)
                     /locus_tag="IV353_001859"
     CDS             complement(5767..5997)
                     /locus_tag="IV353_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010101.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYIAAKKYFTAERK"
     gene            complement(6020..6826)
                     /locus_tag="IV353_001860"
     CDS             complement(6020..6826)
                     /locus_tag="IV353_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010102.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            complement(6837..7481)
                     /locus_tag="IV353_001861"
     CDS             complement(6837..7481)
                     /locus_tag="IV353_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010103.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            complement(7497..8702)
                     /locus_tag="IV353_001862"
     CDS             complement(7497..8702)
                     /locus_tag="IV353_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9010104.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            complement(8702..10726)
                     /locus_tag="IV353_001863"
     CDS             complement(8702..10726)
                     /locus_tag="IV353_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9010105.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIIVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            complement(10889..11482)
                     /locus_tag="IV353_001864"
     CDS             complement(10889..11482)
                     /locus_tag="IV353_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010106.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFGIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(11483..11815)
                     /locus_tag="IV353_001865"
     CDS             complement(11483..11815)
                     /locus_tag="IV353_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010107.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(11867..14386)
                     /locus_tag="IV353_001866"
     CDS             complement(11867..14386)
                     /locus_tag="IV353_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010108.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAAEMADNDKSGLSDLQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLSKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKS"
     gene            complement(14383..16995)
                     /locus_tag="IV353_001867"
     CDS             complement(14383..16995)
                     /locus_tag="IV353_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9010109.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            complement(16995..>17725)
                     /locus_tag="IV353_001868"
     CDS             complement(16995..>17725)
                     /locus_tag="IV353_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010110.1"
                     /translation="LNKLYQTTTEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQ
                     EEKQRQKEKEREQLEIRKREQDLAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQ
                     AQLNYLAQPQPVAAVQTPVNNQANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNH
                     INQSSQADSVATTASIKEEAQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSK
                     AKEGSSDSTVQSNTSESSESNEVK"
BASE COUNT         4835 a         3559 c         2741 g         6590 t
ORIGIN      
        1 aatggataat cttctgccgc atcgacgaac gtcttcgtag tcagatttaa cgttaaaata
       61 tcgccacttt gatacgcttt tgccgctttg tctgtgacga caaaagctct gcctgtttga
      121 atttctgtca caaaaacgag attgtctctg cttttttcaa caacaactcc aatagttgaa
      181 taagccattt cttcttcctc ctttcttttg atataatgta gacggaaatc gcaccactca
      241 atgaattggc ggaaaacaat tcattgaaaa aaagagtcct ggacaaatta tgttcaggac
      301 tcttttactt cttttattca cttaacaact aacattctgt gagtgaaata gactcttcaa
      361 tgcttttaca cgggaagtta ttgatcccct cttcattcat ttcccaagta gtataataag
      421 gagctaactt gccctcatat tcttgaatga taaaatattt ttcacccttc acatgattac
      481 aacagtacac accagtagct gggtactcaa cgactaaaaa ttgatttgga tcaaactgtt
      541 cgtttctcaa catttctctt atccgaaaca aatgctcttt ttctaaacaa tcgggaagtt
      601 gttcgattcg ttcttctctt aatgatcctt ctataacttt ccattgatct aaatattgtt
      661 tcattgctat tcctcctact gtctttcttg ttccatttga attcttcgtt gttgctcttc
      721 gtatgcttta cttgcgtggt atttttcttc atgggcatta atcgttcgat ttaattgacg
      781 caaactccgt tgtgtgacaa ctctttgttg gaattgtttc cttcgcttca taaattcttc
      841 gtaactatac ttgtattcat ttgttggcaa ctgtttaagt gttcgaaagg atcgttcatt
      901 gtaaatagat tgcatctgat cgacaccttc tattaatggt tttatttgac tatcctgtcg
      961 ctttagttct gttaggatgg catttcctgt gcgctttttt aaatcaaaga gggcgttttt
     1021 ttctgcatcc tttgattctc ttttgctttc cccataaaga ctttttgctt cttctttcaa
     1081 tgcttttgaa atctgtatat attcttgata ttcaggttct tttgttaaaa catatttggt
     1141 taactcatcg agtttctctc ttgtgtatgg tgaaagtttg gttcgttttt catccccgta
     1201 attccattcc gaacgtttgt caggtagggt tttatatact tcatttaata agttcattgc
     1261 gtgtcgatcg taacgcgctg gctcttttac cacttttgtt aaggtgttac gcaaatcact
     1321 tttccgcgtc aataaatttc gtttctccat accaatcatt cgatcctttt cataagaaaa
     1381 acgttctaac tcatgtacat aattggtctt cattgcgtct aatgttgctt gtcttctctt
     1441 acctttcggt acgatataac tcacaccatt ttcaacctcc gtgtaaggtt tacgcgtatt
     1501 ttctttttca acaactgcaa aatgaatatg gatatgtttg gtattacggt gaatcgttgc
     1561 catccagtag cctgtttcgt tcagttgctc tttctcgaat aatgtctcca tcatttttct
     1621 tccagctgct tttaatcctt cttcatctaa agtatctgtt ttgggatcgt acaatcctat
     1681 tttttcaaga tacgaattat catgagagac aacatcttga taaatgattg agcctttttg
     1741 ttgtcctctt tggaagattt gcttaatctc cttcagatca cttcctttca cttcgtctga
     1801 agccatagaa aaaactccag gaaggtttcc tgaagctttt ttgtgtggct ctgtttgata
     1861 tttttttatt gcttggttca agcgttggta ttctttttgt tcactttgcg tcagttcttt
     1921 cttttgttct aacgcatagt gacgggtcat ataagaaaga tatttggtaa aatcttgttc
     1981 ctctaacttg tcaaagttga ttccatttcc catcaaagat tgagcttcat tcgacgtttc
     2041 gctgaattct ttatccgtct tactctccga aaaagtttct ccttcaggaa tataaaaatc
     2101 ctctaaagca ttcttgattc gttgcagttc ctgttcttct tcaagcgtac gtcgttccat
     2161 ttcttccagc gctttttctc ttgtcatata atcaatgtat gttgaaaaag attttttatt
     2221 gggcaaggta aattgacaag tgagaatgac tgcaggcgat ttattcatca tgttcccctt
     2281 ctacttttta tctttttaaa aactttgttg gatcaaacgg gtctactctg gcatgaacag
     2341 attcatttat tggtttcttg atcttaggag aatacttttt cgtttgattc gcttttattt
     2401 tcccttccac acgctctttt gcttcttgat aaacagttga atttgtacca attagtattc
     2461 ctgtatcttt caaatgaaga tccgcaaact ctgaaaccat ttcggttaaa atagaaattt
     2521 cctttcccat tgcattcaac tttgcgttcg tcatattatt atttttttca atttctggat
     2581 cgtaattcat acataaaaat cgaatggctt cagaataatt ttttaccgta ggttgagttt
     2641 ccataatatg ttttaaaact tcatcgtgcg cttcataaaa tgtaatatgc ttttctatcg
     2701 ctttaaccat agaaccttat ctccttccta atgtgtgatt aatatttcaa ttaagttctt
     2761 gacctcgttc gtgtatgctt ccaaaacttt tgtattatgc agctgaacat ctgtaaattt
     2821 ttccaataaa cgatcaaatt gatctttctt gtagtccatt aaaggacgtt caaagtcgtt
     2881 ttcaatcaat agttttagat aatcatttcg actccatttt tgaccagttt gttctgttaa
     2941 ttctacgcat ctacgatcaa tttcttgaac atatttttta ttaatacctc ttactttaat
     3001 ctctttaatt tccattttca ccctccgaaa aaagtatgga tagtctatgg agtctatcca
     3061 tgtgctatcc atgtatagct gttttactat ccatacacta tccataaatc gaatattttg
     3121 tacccacttt tgttttagag aaacgtaata ggcacagcct attggaacac tttttttaaa
     3181 aagtgttccc acgtttcctt atgctcttta aaaattaaga agaacttgag gtgagatcat
     3241 ttgtacggaa tagctctaac aacctttttg atctcatcaa gttcttgttt atctggcaat
     3301 gaatcgtatt ttccatagca ctgtaccact tcataattgc catattttct tttctctatt
     3361 tcaactgtat aacgatgctt ctgccgttca tatttccaaa tagttgaacc acctctttgt
     3421 attttttcta agtaattata tacacaatgg tgcatagttg atccttccat aaagagttct
     3481 ttatttgttt taatcaaatt aaaatgagga tgattttgta aatgtttgac aagcaatctg
     3541 tatttaccat gttgtttcaa tttgaatgtc tttaatcttt tagcttgttt caaattatat
     3601 tcttcaacaa tttgattatg ctgtcttaat aatcctcgtt ctgttttaaa attaaattct
     3661 agtttctgat ttaaataaaa actcatgtcc attaaatcag atagaataat cgttgcaaaa
     3721 tttgatagaa tacagttttg aacataacta actaataagt cttttacgaa tttacgactg
     3781 tttttccttg cacacataaa tatttctgaa ggaaacagat cattgtcttt ttgttctatc
     3841 gcagcttcta ttcttctaaa ctgttttctg gttactcttg gttttaactt aatcagcgca
     3901 taagtatgac gtagaggata tttatttacc ttcttagtaa atttttttga cggatacttc
     3961 ttacacaata attcatattt attttttgct tctataatct cgacccaagt gaaaggtaat
     4021 gaatcttttt ctttcatcat ttctttaata aacggattga ttttttgaaa aatagattca
     4081 catgaaccat ccatgaatgt gaggggtctc tcgtaggata atgtaatttg tttcttccct
     4141 cttcgttcta gaatggttaa ttcatttggt tcatcttgta aaaacaaatg ttgaaaatca
     4201 ttatatcgga tataaaatgg tcgtgactca aaataaatag aacgaccaat actttcaaat
     4261 tttagttgag taaatttgag actatcgtat tcttcaccag cacatattaa aactccctga
     4321 tcatcttcaa tcagtcggcg gtttctttga taggtgactc ttactaaata ttctaagttt
     4381 gttgttccgt caaattttat ttctgaaata gtttctttat ttttactgat ataatttttg
     4441 aatcgtctta aatgggaaat gatttttgat tctttgggat caatgattct tccttcacga
     4501 caaataaatg tcaaatagtc gtattctttt tctatttgga ataatcccat caattcatca
     4561 ttatcttcaa ttgaaaattg ttgaaaatga ttactgatat cttcaaataa atggttcaaa
     4621 ttgatcgaac tcattgaagc tcatcttctt ccatagaaag ctttttagat acttctaaag
     4681 catcactttt ccagccaatg tatggtttgt tttgtggaat atttcttcca tctaacgaca
     4741 attttccaat accatatctt agacttgctt cgacttcata aaaattgtag acctcttttc
     4801 ttcggtcaat tgctttcgac tcctggccat cttttaacgt agttctgatg cccttaacgg
     4861 tcgcttcata gatactcgtg tgaacaatac ctgtattgat caattcgtca tatcttccaa
     4921 aatcaacata gtactcataa ccaaaaagtg tcggaatcag gtcaatctca atttcttttt
     4981 caattgaacg tttaataagt ttcgtggata tatacccgaa taaaaagaga ataataccca
     5041 cttcaaacaa atacatgtac ccatttacaa agtgaacaaa cgtcattaaa aattccttca
     5101 gtccttttgt tgttcttcca aattgttcga tttgttttat taaagttgct agaattgcat
     5161 ttacaataat taaatcgaat agacttttga aaagttttcc tgctgttaat ttttgcctgg
     5221 taaattgcat ggctatttga gtgaaaatag taaatagcat gacaaaaatc aacgtcatta
     5281 gcactgtaaa aatgtctgat gctgcaatcc tattgggatc atttaataag taataagccg
     5341 ttccaaatac tacaatcgag taaatgatta aatcaagtat aaaacgagcc aaatagctac
     5401 aaagtttacc taatggccat aattgtagaa tccaaaattc ttttcgcttg ctatttcttt
     5461 tcaatggtaa ccaagtatgt tccatccagt cgctcgattt catttcgatt tcttctctaa
     5521 actctttcct cttatcttgc caatgagaag gcatgaattc gcggatttct aaacataaaa
     5581 agatcaacat aatgaacgac aatagactca tgaaccaatt aaatgataaa tctttattga
     5641 taaaccaaag aaaaattgta ccgacgataa cggctaaact aaagaaaaaa acagctatac
     5701 ctacggtgat atagctgatc cataaacatt tttgatatac ttttttcaca taaaatcctc
     5761 ctgtttttat tttctttcag ccgtaaaata tttttttgca gctatatata gagcttcaaa
     5821 tgatctgaat tttaaggtat ctaaactctt tctaccattc ttcaactcag atattgttgt
     5881 gttagataaa ttcacttctt gacatatttc tttagctttt acaactttga gataggcttt
     5941 aatcaatact ttctgctcag cgaaagtatc tttgccgacc aagttcgctc gcttcatttc
     6001 ttttactcca cctattcttc taaatatttt cttttgtcat acgatcaatt ttttcttgaa
     6061 gtttttgaat gatctcttct tgatctttca tttgcgtttt cgatgtttca atttcttgct
     6121 ggtaactatt aatcttattt tcagtatttt cctgatcgtc tgcaacttga taaggcaact
     6181 gatcttttag tttcttcact aattcttcat tcaaatctat ggttgcttgg aaagattcaa
     6241 tttgtttttg tgctgctttt tgttctttct tgtatccttt cactttgtaa ttgatcgcat
     6301 gttgttcata atcttcgtct ttcacgtgat ctttgacctt atcttcgtga acgtaataaa
     6361 tcagatcttg cggttcttgc atatccacct ttggatcaat tttttcaggg ataatggtta
     6421 atcgcatagc aatatattct tctggtacat gcgatagctt cactacaaaa taatcatcag
     6481 acaccttgat cactttggct tttattgctt gataatcccc tttagctgtc tttacttcta
     6541 cacgataatt aagattagct agttctttta ataagctctg atcttcatcg tcgatcgcaa
     6601 attcaaaaac taattgctgg ttcgctcctt tttcttttga aaccatcgtc agttccttcg
     6661 tgtttaaaaa ggcgttcgtg cttacttcat ctggtatgtc taccttctta tgcgtgtaaa
     6721 tcggactagc aagaatcaaa aagccgagaa tcataaagat tcccagccat ctaagataac
     6781 tttttcgttt tttaaaacgt tgtcttgctc cttttttctt actcattttc ttcctcctaa
     6841 cgagtgaaat aactaaagtc tgtgactttc cattgaccat ctacctgttt ttcagtgaca
     6901 gttaatccac cttttatttt ttgtggtttg acatctttga attgaggatt gccaattgta
     6961 taatcaacaa ataccacaaa gttttgttcc tcttttacac tgcttgtttg agcgtaagtt
     7021 tgcacgtcac ctaatgtaaa ggagtaactt ccgtaatcat catgttggtg atcatcgtct
     7081 gcaggactta atttgtcgat cacttcttgg gttgcgacat cttttagttt ttccgttaat
     7141 tcatttctgc ttaatgattt atcaatggcc aattgaccga actcttctgc cgtctttctt
     7201 tgtgcttcag tgacttcctc ttttgctttt gtgccatctt gttgcttcag ctgatcgatt
     7261 tcttggtttt tctcattgat ggtttgattt aacttgtcga tcttcgcctg tgcctcagag
     7321 gaattttttt tgagcttctc agacgcgttt ttagccactt cttcaatttt aaaggtattt
     7381 gtctcttgtt cttctttaag ctgccaaaac aacgcgatat tgccgattaa actaacgcta
     7441 attgcagctg ttaagacgat ttttaaagtt ttattttcca tattttttct cctgatctat
     7501 tttttgctgc tattcaaaaa tggtaatgga tcgatccatg taccatcatc tgtgaatgcg
     7561 tgtccttgtg cttgacgcca atccattcga gtgaaaccta aatgcaagtg tgctgtatct
     7621 cgtatcccga ttacttgacc aactttcact tgatctccaa ccttcacacg agaatttcct
     7681 gtactattcg caaattcttg atacaccata ttcaaaccgt cgtaattaat cacgatcaca
     7741 catgcaccta aacctgaaat accaggattg ccaacgtaca cgacttttcc tccatgcacc
     7801 gcgtgaattt cacttcctgg atgatcgact gaaccaaaat ctaatccgtc atggaaacca
     7861 tttggtcgga actctccccc tggattcgtt ccaaataatt gaccaccgct aaaggaactt
     7921 ttatctaaac tgctgccagg gaatggattc gtccattcac cagtgatcgt gccgttgtta
     7981 ttgccacctt caccccaatg tttgatggcg aattcatcat attcggttaa tttattagaa
     8041 gtgataattg acatacaacc tgtataatac ggactatctt ccgtaattcc tgcagcggca
     8101 taaccgcctc gtcctaagat caataagctt gcgccagggc ttttatttcc aacacaagca
     8161 ttaaaattgg attgtgtggc taccataaat ccgtaatagg aaaatgcagc ttttgagttg
     8221 ggaaacttca tgtaccagcc gccttcaatg ccaccaattc cacgcgcagt tcctttggga
     8281 aataaacagc catcgaacca agtaatccca aaataattga gatcatttct tgcagacgcc
     8341 gacgtaccaa acgctgattc atacccgtat tgcgaaagga tcacacttgg taataattta
     8401 tatttgactg ctgcacctac agcatattgc gcaatctcat cggaaaaagg gcctcttcct
     8461 ttgatttctt tataaatggg taatccttcc gaatattctc ctgtaaaagc ttcgccacca
     8521 ctacttgaat ctgaatcaga attatcaatc gaacccgccg caagtattag aaaaagggtc
     8581 acgaacacag tgatcaaaat agtaatcaaa cctgcagaag taccaaacaa ccacaaaaat
     8641 aagcgtcgct ttgcacgacg cttaacatac tgcttgatta aactttcatt ttggttttcc
     8701 attattttcc tccatcaaac aattttaatt gacgtttgtt cggactaaaa tgaacacgaa
     8761 tggtttcttt tgagccaaaa ttgatcacag cgtctccttg attttgttga ggaatggatt
     8821 ctaattctga gtttgtcatt gatttaccaa ctaatccttc tatatgacgt aaaatagatt
     8881 catccatacg cataaagatc ttcgtagaag aaaattcaaa gactttcttc agtttcgaga
     8941 agttatcaga agacatattt tctggcacca tttcttgtgg ggattgtgta gctaaaacag
     9001 tcattgcttc tactttacgc atttctcgtt gaaagtttgt gacataattt accgtttcaa
     9061 taaaatctgg attaatcgca ttatggcact catcaatcat cgccaagaag catttgaagt
     9121 cttgttcttc catttgtttt tctttaatta aacgtcgttg tcttcttcct tgaatcaatg
     9181 cctgattcca aatcaacgtc atagccatgt acatttgaca ttgttgtacc ccacgatcta
     9241 aacctgtaat agagtcaatg tcaaaaaaga ccaactgttg ttggtcaagg tgtcgaatcg
     9301 tactatgacc atcaaaaatc atgccatacg tctcacataa ggattctacg atggattcca
     9361 tttcttcttt tcgattcttt ttctcttggg aagcacctgg taattccagt tgtcctaaaa
     9421 agtctaaaaa ttctttgaac gtcggataag cttctggatc tttccctgtg acttgcgtct
     9481 ccgtatttga acgcgagttt gaggaagtga aaccgcgtgg tactgttcca tactcttcat
     9541 aaaaacgatt taaataggtc ttaaattctg ctttcatcgt tccttttagc tgaggactca
     9601 ctaactgtac ctgatagacc actttgtcta agtgcgtttg ataggacgct gcttcattga
     9661 tcttcaaatc tttgactgat ccatcgtcat ataaactgga agcttcatca tacgtacgtg
     9721 tggcaaatat ttctaaagga ttgatcatcc cgtcagatcc gtctaaacgc actataatgc
     9781 cgttatattc ctttaaaaag ggaataaagt cttttccctt ttcaaagccc caaataaagt
     9841 ctcctgcagc taaatgggct tccgcgatca ttttaagaac ggtcgattta ccagctccca
     9901 tcattcctag aagcaaggta ttggtagaca accgcacact atttttatac gtggcattcc
     9961 aaacaaacgg gccgcctgtc atggtttccc catattgtgt tccacctgga tcttttagcg
    10021 tttggtggtg gaacgggaaa cctccgccaa tgtttagcga tggtagtgat tgccctttga
    10081 tatttccaaa ccattcagct tgttcatcga acatggtgaa catcgattgc cagtctcttt
    10141 tcgttttgtg cagataggtc actgcacgat aatccatccc tttgagattt tcgatcagtt
    10201 cctgtgctcg ttcatctact tcttgaatcg tgtttccgta aacatgtact ctcactttga
    10261 cttgtttaga aatttcccca ttagaagtca attttcgggc atattccgct agttctaggt
    10321 attcttccgc gcttttattt cggtcagtcg cttttcgttc attatctgac cgatcattta
    10381 attcatctaa ggcacgattg atttgatcaa tcactttttg cttttcatct gtgctaacat
    10441 ctagtgttgc cactgtatga ggattgttca tgagattggc caaccaaaaa agcgtaggat
    10501 tttttgtgta gtgataaact gtcacacatc gtgaataacc atttccatag gtcacatact
    10561 cttcatcgaa atttaatcca ccttgtggca tcacttgttt taaaaagtct aaatcatacc
    10621 ctttggattt taaaatttct atttcccgat ttcctagttg ctttaatgga tcaagtacct
    10681 tttgatcatt ttgtggtgaa aaagctgttt taattgcttc taacatgtac agtcacctct
    10741 tcttgtttga ttctttactt ggttaaaaga ttttcctttt cccaacttac ccaggcttaa
    10801 agtgtgagga aattttctag tggtctcttc tttcggaaca tctttttcta accatatcaa
    10861 cggatatttc attctctctt ccccattcct actcatcatt catattattc attcgataaa
    10921 gaatcttgat caattctttt ttgtagagtg gttttaaccc taaaatgcca ctagatcgca
    10981 ttaactgacg gtttctggtt tgcaaatcct tgattgtctt tccttcaatg ccgaaaaaga
    11041 agcttaaatc agggcgttcc tgttctgctc gtttcaattc ctggatttcc tctactaatt
    11101 tcttttcata ttttcttaat cgctgtagat ttcttcctaa ggactgttcc atacgatttt
    11161 tatctaataa ctgccgcttt tgcacgattt gctccgttag atccgtttct gtggtcaaac
    11221 taactaactt aatcgttggc acatacgtac gcatgacagt ggtcaactga ttcattcgtt
    11281 ccagtttctc ttgttcattt aatccttcca catctgtaga aactacacga taataacgtt
    11341 gaaaatgatc ttctttagta attaagactc cctcttctgt acaggattta taaggaaata
    11401 agtccaagat agagcgctct tctggttttc caaactgcaa aatggatttt ctgtcaactg
    11461 aattttgacc tttgactcgc atttattcat cctccttata cgtaccatga taatttcggt
    11521 taggatctaa cgcatgataa tggttcttgt ccgtaaaaaa aatagactcg ataatcgcaa
    11581 aaatctggcg ttgacctggg ttattatagg gtcgccaaac aatcactaaa gctatcgcaa
    11641 tccataagcc ataattcaag agtgcaaaaa agagtggtaa gtggacaaga ttattgacta
    11701 ttttcgccca aaaaaagcaa ccaccaatcg ttaatagatc taaaaagaaa aaggcgaaaa
    11761 acttccattc cccactaaat cttggtggat ttttcatgtt aatatattta tccataaatg
    11821 atttctcctt ttcatacata gaaaaaaggc ctaactcgta ggccttttat gatttgatat
    11881 ccttcaataa ttgttctttc aattcattgt tcctgttttt agtttctctt gttacattta
    11941 tttttgttgg tgcagtaaat gccacatttg ctggcttgta acctgtatga acgttttgaa
    12001 taggttttgg tgcagcttct cttgtcacaa cattttgaac ggacgcattc cctgtctcac
    12061 cactttcaga taaattgtaa gaaacagttg tcagatttcc attttcaggc aaacgacttg
    12121 gtttcatttg aacagtacct gcaggtgatt gatgaacaaa actttgatca ttatccttga
    12181 attgattcaa acgactatct ttcattggta aattttgtgc cttaggtaaa ccaattttcg
    12241 ttgatttttc agttccaact tcgattccat tatcatttgg cagaacgatg gaatcctcat
    12301 ccgtttgtac ttcttgattt cgttttgata aatctgaaat gcgtttttca ttttcggagt
    12361 taacagatct ataccgatat tgttctggat cagtttttgg taactgtccc tcattttctg
    12421 ctttgtcttc ttgatttact tttgataaat ctgaaattcg cttctcattt tcaaggtcac
    12481 tgttaggttt atagcgatat tgttctggat cagtttttgg taactgtcca ttttttcctt
    12541 cttctgaatt tctttcttct atggaatttt tctcttcaat cgaattttca ttagagtgca
    12601 acgcttcttg gtgatctttg actgaaggaa tcactatacc ttcttgattt tctttttcgg
    12661 ttgttgtatt agcttttact gctgcattaa cttgtccttc accatacgca tcttttagtc
    12721 cttctttgac ttctgaagct ttttcttttt gtttttctag tgcttcggat ggattcttca
    12781 cagcttcagc caattcttta gctctcgtgc cgtctaattt ctcttctaag gtgtctttgg
    12841 ctttttctgt aagatcttca gctgttcctt ttaacccacg ttcattgacg taaccaagac
    12901 cttctgcggc tttagaaaca ccatttttcg ctttcccgaa catttcggaa actgttttct
    12961 tactattagg tacagttgta gctttatcgt ctgattgttc ttccccgtta tcaggggtaa
    13021 catcttttcg atggccaaat ccatttttac gaatatcatt taaatggctt aaaccactct
    13081 taccagcact tgcaaggttt ttagctccgc tacccacatt tttcgcaata tttcctaagg
    13141 caaacatgct aagaagagaa ttactgccgt ttttcagact cgtatcaaca ccgaaaatct
    13201 tcgtaaccga ttgagagcct ttgaatagcg caatagttag cacaattaaa gcaacaacat
    13261 acaagaatcc attgatcttt cctgccgcat gcaaatcacc gatacctgaa agcattaatg
    13321 tatagattcg taattctaaa atcgtaaacg ccaataacaa gaaggattga gaaatatctt
    13381 ctatcgcact tttcatccgt tggcctgtct caaagtccgt tgacgcggca atcactaaat
    13441 agaatttttt aaataccagc tcaataatgc aagtaacgat cgtaaatagg ataaataaat
    13501 aggccaccgc caaagaagat tcaccagcta agataatacc tggtttacta ctgtatcgac
    13561 gataccctga agtatagaca tattttgcta agccttcgtc actaatttga acaggaattt
    13621 ctttcccatt atcatctagt tccaatcggt attgtaatgc ctttaaccga tccttttgta
    13681 aatctgacaa tccactttta tcgttatccg ccatctcagc ggcttgatca ggagtaataa
    13741 tcccattgat attcgctgtt ttaaaatttt tcgctgttaa agcatttctt ttatctgttg
    13801 gtagggattc tactttattc gggtcagttg ctagtaacgt actgacatcg atcacattat
    13861 tttgaataat ttgaaaagca ggtgatcccg ttttatctgc tttatttgct gcagtgacgt
    13921 ctccgtaaaa gcttttactg atatttaacc cttcatcgat taaagaacta ccaccaaaaa
    13981 tcaagatcat ggccataaat aaattaacca agatattttt gacttttggt gggtgtttac
    14041 tcataaaaaa tttcaagcct aaataaatta atgtgacgac acaaaaaaga gccaatacct
    14101 tactaatcaa actttgaaat agtgcattta ccccagcggc gttcaataag tcttttaaat
    14161 caaagctttg ataaatggtt ttttctagta aagtattgag attatataat cctttaatca
    14221 atccccacag aaaagtgttc caaatccaac taaatacatc ttgatactgc agataattac
    14281 cataatcttg taagactttc gttacggttt tggggtttgc atccccttct ccgaataagc
    14341 ctgaccacca gtcggctatg ccagaaaaaa ttcctccaat gatcattctt tcaccacctt
    14401 ttcagattcc aataacaacc gtttcccaat catttcagaa atttctttat ttttgatcaa
    14461 cttttcaaca tattcttgaa gtcgatctgc tgcttgttct tcgtaaaatt gaattaacaa
    14521 ttttttagcc gtctcattct caagagattg aacgatttta taaaacatat tttcttcgtt
    14581 tttttcttct aattgatcat gcagtccatg atagagattt ctagcatatt catctggtag
    14641 gaaataatca taatcaattt gtaaagcctt aagatccaag tccgcatgtg tacaatgtat
    14701 gtctatatca ttgatatccc acgatggatt gaaatcttca atatattgat aagcataagg
    14761 aagattcgtt tccccatgat taaaaatagg atacggccgt attttatgac ctttcaaatc
    14821 ttgtcgtttt aaaggagcga gtacaatcgt ttcaccttct aaaaaagtag aaattctttc
    14881 aggagttaag atacgatctg aatcaacatt gcgattgata tgagtattca tacctaatga
    14941 attgatggta ctgttctctt gttcaaccgt cttattcccc acttttttag aaaattcttc
    15001 gatggtatta tcatctgtcg acataatata aatatgtgtc tgacaatttt ctttgataat
    15061 cgctgcgttt tgttctccat acttcgcata aagttgctga taagattgaa taaacagatc
    15121 ccacaacatg tttcggccag ctgtgaccgt catgattcct tccatattat ccattggtgg
    15181 catattccca aattcatcta aaataaaatg aatcctgcgg tgacatttat ctcctttagt
    15241 cttaacacat tgcgtagata attctttata taactgacta agaaaaatac taacaatgat
    15301 atggttagat ggatcataat caggtgtaag cataaaaata gccattggtt tatcggtata
    15361 gtgcatccga atagactcta ctaataagtt acttttctcc tcatctaatg ttagaacggc
    15421 ttctttttct aattctggca aatgggtttc tcctcgtttt ttccgataaa cgatattacc
    15481 ctcttcgtct ttcaatactc tttgagaaaa ttcataccaa gccacatatt cttttccttg
    15541 atgatcgtat tttaaacaaa cttgatcacc tgcaactaaa tgacaatcaa aattgatttc
    15601 acaaaatcct ttatagccta ctttgacacg ttcttcatga aatttctttc ccttatgata
    15661 aaattctact acgactcgtt gatttaaaag actttcatgt aatttcaaat ctaagtattt
    15721 tggaaaccca accgatttat aatcaatcgt tgattgagaa gtcatctttg caattttcgt
    15781 tagagtaaat ggattcaatc ccgtgattgc cgttgataaa atatttccct tcgctttatc
    15841 agatccaatt ttcgttgccc catattgttt ttttgcaatg tttccaggtg gcaactgatt
    15901 cataaattca tctaaggcat tcaactgttt gaattcattg tctggatctg gataataatt
    15961 aaaaccgcca atctcagaaa gaagctcaac gagattattc ggtgtcaatt tggagtaatc
    16021 tcctgtttga tcagcttgtt ctaacaacgc aaaaataagc gcattgacag ctttctgcgc
    16081 gttttcgtta aagtatttgt tatctgaagc gttggcatca taataaatcg tatttgtgaa
    16141 agtattggtt aactgtgttg cagtatctat gtctcctcgt ttccagtatt tcacgatgag
    16201 tgccaatgga ttatcactca ttgattgcag tggatcggtg atattaaagg catatacatc
    16261 ataccctcta gcttctagtt catctttact accaacatat aattctgttt ttggatcatt
    16321 aacgaccatt gaactttgtt tttctgcacg agataaattg tcaatggtgg ctaacacttc
    16381 aatctgacct ttccctgatc gagaggtacc aacaatacac gtattaacag tatctgtttg
    16441 aatgtaccaa tattttttgt aatgtgtgat tggaatacct ccataacctg gaaaagtagt
    16501 ttttgtttca ggtatttgcg gataaatctg ttggatttct tttaaggtca ttaatcgatt
    16561 atcccctttt tgtccatctg ctagaggacg aaaccacatg tccattttca atcccataag
    16621 tacagctgga accacacaaa ctgtcgaaat tgctaaacgt gtacccaaac tagttgtagg
    16681 agtaaatgga ttccaaaaac cagctgaatc aaaatgaaag acctgtggca tttcctgtaa
    16741 ttgaaacaga tttattcctt gtaagcttcc cttaacaatt tccaatcggt tgaaaacttc
    16801 tggtactctt gttactaaat actctaaagg gaggtataca agtccactat aaaaaatctt
    16861 tttcgaaatg cgtttgtcat gtttttctgg tagctcttct cctgagggag tatagcgata
    16921 gaaatgagaa agatacctat tcttttctaa attgaatcgt ttgtcttttc gcttcgttgt
    16981 ttctctaaca cgcattattt cacctcattt gattctgaac tttcggaagt gtttgattgt
    17041 acagttgaat cgcttgatcc ttctttagcc ttactagaat cactctcttt gctattttcc
    17101 atgcttgact ctgttgttga ctgattggta cgattcaata attcttctaa ttgttgttca
    17161 tactcttttc ttgcttgttc ctgggcttct tccttgattg acgccgttgt ggctacagaa
    17221 tcagcctgtg aagattgatt gatatgatta aaagcaaata ctgttcctcc acctagaagc
    17281 aagcttaaac ttgcaataca tcccaccatc catccgttaa aggaacgctg gggtgaagga
    17341 gttaaatttg cttggttgtt cactggcgtt tgaacagcag ctactggttg aggttgtgct
    17401 agataattca actgtgcttg acgcaatgta tccatttgtt cttgacgttc cttacgagca
    17461 gctgcttctt tttccttttc ttgctctaac cgttgttttt ctatcgccat tttttgttcc
    17521 gcaagatcct gttcccgttt tcttatttct aattgttcac gctctttttc tttttgccgt
    17581 tgtttttctt cttgttcttt caattcttgt tgttgaattt catactcttt tttccatttt
    17641 ggaatttgta cttgtaattc ttgctggatc tgttcgtgtt tttccataag aacttcagtc
    17701 gttgtttgat atagcttatt cagtt
//
LOCUS       DACDLI010000052        17644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDLI010000052 DACDLI010000000
VERSION     DACDLI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.052"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            279..2924
                     /locus_tag="IV353_001869"
     CDS             279..2924
                     /locus_tag="IV353_001869"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9010111.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3234..4547
                     /locus_tag="IV353_001870"
     CDS             3234..4547
                     /locus_tag="IV353_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9010112.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4537..5190
                     /locus_tag="IV353_001871"
     CDS             4537..5190
                     /locus_tag="IV353_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9010113.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5245..5937
                     /locus_tag="IV353_001872"
     CDS             5245..5937
                     /locus_tag="IV353_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9010114.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6087..6287
                     /locus_tag="IV353_001873"
     CDS             6087..6287
                     /locus_tag="IV353_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9010115.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(6541..8220)
                     /locus_tag="IV353_001874"
     CDS             complement(6541..8220)
                     /locus_tag="IV353_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9010116.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(8222..8434)
                     /locus_tag="IV353_001875"
     CDS             complement(8222..8434)
                     /locus_tag="IV353_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9010117.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            8827..10557
                     /gene="efrA"
                     /locus_tag="IV353_001876"
     CDS             8827..10557
                     /gene="efrA"
                     /locus_tag="IV353_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9010118.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            10554..12314
                     /gene="efrB"
                     /locus_tag="IV353_001877"
     CDS             10554..12314
                     /gene="efrB"
                     /locus_tag="IV353_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9010119.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            12409..12606
                     /locus_tag="IV353_001878"
     CDS             12409..12606
                     /locus_tag="IV353_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010120.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            12759..13244
                     /locus_tag="IV353_001879"
     CDS             12759..13244
                     /locus_tag="IV353_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9010121.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(13348..13941)
                     /gene="clpP"
                     /locus_tag="IV353_001880"
     CDS             complement(13348..13941)
                     /gene="clpP"
                     /locus_tag="IV353_001880"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9010122.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14121..14648
                     /locus_tag="IV353_001881"
     CDS             14121..14648
                     /locus_tag="IV353_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9010123.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            14739..15476
                     /locus_tag="IV353_001882"
     CDS             14739..15476
                     /locus_tag="IV353_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9010124.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            15480..16397
                     /gene="miaA"
                     /locus_tag="IV353_001883"
     CDS             15480..16397
                     /gene="miaA"
                     /locus_tag="IV353_001883"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9010125.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            16399..17628
                     /gene="hflX"
                     /locus_tag="IV353_001884"
     CDS             16399..17628
                     /gene="hflX"
                     /locus_tag="IV353_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9010126.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5691 a         3045 c         3866 g         5042 t
ORIGIN      
        1 ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga
       61 gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc
      121 tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa
      181 tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt
      241 attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat
      301 caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag
      361 atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc
      421 caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc
      481 tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg
      541 caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt
      601 atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga
      661 gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt
      721 ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa
      781 aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc
      841 tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg
      901 aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg
      961 gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa
     1021 aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata
     1081 tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg
     1141 taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg
     1201 aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag
     1261 atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt
     1321 ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg
     1381 cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc
     1441 ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct
     1501 cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa
     1561 tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt
     1621 tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg
     1681 aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca
     1741 ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat
     1801 ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat
     1861 cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc
     1921 gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa
     1981 tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga
     2041 atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc
     2101 ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt
     2161 ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact
     2221 ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca
     2281 cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc
     2341 cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg
     2401 cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag
     2461 tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt
     2521 ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg
     2581 aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga
     2641 tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt
     2701 tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt
     2761 ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg
     2821 cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa
     2881 cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag
     2941 tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt
     3001 ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc
     3061 ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc
     3121 aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta
     3181 tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga
     3241 ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg
     3301 accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc
     3361 atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag
     3421 aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga
     3481 tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc
     3541 agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga
     3601 cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg
     3661 aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa
     3721 taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg
     3781 cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag
     3841 aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg
     3901 gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat
     3961 tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag
     4021 ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc
     4081 ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg
     4141 agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga
     4201 accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt
     4261 ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg
     4321 tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag
     4381 atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat
     4441 tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag
     4501 aagttcgcca tttattaata caattaggag gagatcatga agtataatgc agtgattttt
     4561 gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta
     4621 gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca
     4681 gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta
     4741 caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta
     4801 caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa
     4861 gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg
     4921 tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca
     4981 gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta
     5041 gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta
     5101 ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct
     5161 ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag
     5221 ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc
     5281 tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt
     5341 ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat
     5401 ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa
     5461 gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt
     5521 cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg
     5581 gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat
     5641 ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag
     5701 tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc
     5761 gttttagcgc ataatcatcc ttctggtaat gttattccat cgcagcagga tatgaatttt
     5821 accaaaagga tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc
     5881 atcggacgca aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt
     5941 gctttcttgc aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata
     6001 gaagttgaaa catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt
     6061 gcttttggca cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg
     6121 aaaaaggatt tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag
     6181 ctatccaagg tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg
     6241 aagaatcaga ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa
     6301 tcaaattgat ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg
     6361 ccacaagaga caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt
     6421 aagaggctat gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc
     6481 atgtttttca tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat
     6541 ttattcttca tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc
     6601 gaataagaaa ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc
     6661 aggtccattc aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat
     6721 ttctcctgat tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat
     6781 ttcttttttc ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag
     6841 gtgacggtca cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac
     6901 atctccagcc acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc
     6961 gccattttcc atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc
     7021 atgtactttc aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa
     7081 acgaagcatt aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat
     7141 ttttccgtga atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact
     7201 tgttgtatta ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg
     7261 gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga
     7321 acctgtacat aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac
     7381 aaatgtgccg tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc
     7441 catgcttcgt ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg
     7501 ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga
     7561 aaagatcttc aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc
     7621 agcatttgta ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt
     7681 atgcaagttt gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac
     7741 gatatttcca gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt
     7801 tctgaaaaag ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg
     7861 gagctctgca tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc
     7921 gagaggacca gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat
     7981 gtggtcttcg tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat
     8041 gtagctgtag tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat
     8101 cccggcatca atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat
     8161 ttcacctaat ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat
     8221 attaaaactc cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt
     8281 ctagaagttg aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt
     8341 ctacatcgct ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta
     8401 cttttgtttc ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa
     8461 gcagtgattt agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg
     8521 tttttcatat gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg
     8581 cttatctgtt cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa
     8641 ttgttattct agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa
     8701 tcaatgttta tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta
     8761 ataggctcgt tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat
     8821 gagaaagtga aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat
     8881 ttcatatgcg tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt
     8941 atgatcgatg taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt
     9001 atgatcgtta ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg
     9061 aggattacga ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt
     9121 tattcgcatc aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac
     9181 gatgcttatc agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca
     9241 atgatgttca ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat
     9301 gtagtaggtg cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa
     9361 ccgttatcta aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat
     9421 ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt
     9481 gctgtagtca atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt
     9541 gcacaaccgg gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg
     9601 gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat
     9661 atattccatg ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga
     9721 gcagctgttt cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa
     9781 gatccaaatg gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca
     9841 tttgcttatc caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct
     9901 ccaggagaga ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa
     9961 ttgatccctc gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg
    10021 agagattatc ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg
    10081 ctatttactg gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa
    10141 gaaatggaac gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat
    10201 ggatttaatg aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga
    10261 ttggcaatag ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt
    10321 tctgcattag attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca
    10381 gaatctactg tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc
    10441 atcgtattaa atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa
    10501 tgcccgattt attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa
    10561 aaaccttttc ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc
    10621 ttttagtgat cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta
    10681 caggacttcc tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt
    10741 cttatatatt caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc
    10801 aattactttc tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa
    10861 gagacataga agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag
    10921 gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag
    10981 cttttattgg tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt
    11041 atatccagcc gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct
    11101 caaaacgagt gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg
    11161 agctaaacgg ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac
    11221 gcgaaaaaga aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt
    11281 ttcgtgcagc attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta
    11341 cttatattgg tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg
    11401 gtcaactaca agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga
    11461 cacaattgtc ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt
    11521 tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag
    11581 gagatgtgac attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag
    11641 atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg
    11701 gcaagacgac attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa
    11761 ggattgatgg aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta
    11821 tggtcttgca agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa
    11881 agcttgatgc gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt
    11941 ttatccgaac gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt
    12001 ctttaggaca aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct
    12061 tgattttaga tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag
    12121 caatggatcg cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa
    12181 tccgtgatgc agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc
    12241 atgagagttt attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg
    12301 aggaagcaga ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat
    12361 tcttgataat ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt
    12421 ttaaatgaaa aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag
    12481 acgaagcctt ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca
    12541 gcacaaatag aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta
    12601 caataaaaac aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca
    12661 ggttttacct gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag
    12721 gattagtttc tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa
    12781 atgccacagt gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg
    12841 ttttacgcgg ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta
    12901 caatcatcca tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac
    12961 atcagtgcat gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca
    13021 cgatattgaa tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa
    13081 cagaaggaaa agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc
    13141 gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga
    13201 taggtcggat gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa
    13261 aataaaaaag gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga
    13321 gcttaaaaaa gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc
    13381 atcgatcaat ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc
    13441 tttttcaatc acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc
    13501 gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc
    13561 gccaagaggt tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt
    13621 accagctgtc agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac
    13681 atctgctttg acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc
    13741 aggagaattg atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa
    13801 ttgggcgatg atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg
    13861 gtctttcaat aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat
    13921 gactgtagga attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac
    13981 cataatttag tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat
    14041 aaaaaactta ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca
    14101 aaacaaggag gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag
    14161 gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac
    14221 agcgtagacg tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata
    14281 aaaagatcaa cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg
    14341 atgcatttga tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag
    14401 cgcaaaatag tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc
    14461 atcgaaaagt gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag
    14521 gcggacgcgt gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa
    14581 aagctcagtt gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt
    14641 ggagctgata ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt
    14701 tcttaatgat acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc
    14761 gcggaagcag cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag
    14821 taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta
    14881 tgcacgacga agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat
    14941 tggaagaact caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta
    15001 aagttcctga attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc
    15061 taatgattga aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg
    15121 aaacattaaa aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt
    15181 ctttggaaaa aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt
    15241 ctgataaaaa gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa
    15301 atacttggtt gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat
    15361 ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa
    15421 taactgattt tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata
    15481 tggaaaaagt attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg
    15541 aactcgcaca actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa
    15601 atctggatat cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt
    15661 tgattgatgt gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc
    15721 gacaattgat taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg
    15781 gtctctatat ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc
    15841 ctgagattag aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga
    15901 atcagttgaa agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca
    15961 aagtgataag agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag
    16021 aagagccaaa gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc
    16081 tttatgaacg aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag
    16141 cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat
    16201 ttttccctta ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac
    16261 attcgagaag atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt
    16321 ggtttgatct tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat
    16381 ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca
    16441 aaccagcgtt acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca
    16501 ggagaagtcg tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc
    16561 ggtaaaggga aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc
    16621 atctttaatc atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt
    16681 ccggtaattg atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa
    16741 gaagggaagc tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg
    16801 caagggaaaa cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca
    16861 aagttagaaa cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg
    16921 aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt
    16981 cagattggac ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca
    17041 caagcggata cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga
    17101 tggcgttttg ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac
    17161 ctgcctacgc aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat
    17221 cttttgctac atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc
    17281 ttaaaattga tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag
    17341 gcagaccaga tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct
    17401 actcagacga aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa
    17461 ctaatgaccc cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg
    17521 agaagacaaa caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt
    17581 aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt
    17641 ttaa
//
LOCUS       DACDLI010000053        17164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDLI010000053 DACDLI010000000
VERSION     DACDLI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.053"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IV353_001885"
     CDS             complement(543..2048)
                     /locus_tag="IV353_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9010127.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IV353_001886"
     CDS             complement(2045..2731)
                     /locus_tag="IV353_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9010128.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV353_001887"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV353_001887"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9010129.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IV353_001888"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IV353_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9010130.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IV353_001889"
     CDS             complement(4752..5321)
                     /locus_tag="IV353_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9010131.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IV353_001890"
     CDS             5507..6409
                     /locus_tag="IV353_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9010132.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IV353_001891"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IV353_001891"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9010133.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IV353_001892"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IV353_001892"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9010134.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IV353_001893"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IV353_001893"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9010135.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IV353_001894"
     CDS             12977..13162
                     /locus_tag="IV353_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9010136.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IV353_001895"
     CDS             complement(13224..14420)
                     /locus_tag="IV353_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9010137.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IV353_001896"
     CDS             complement(14590..16026)
                     /locus_tag="IV353_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9010138.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IV353_001897"
     CDS             16319..17128
                     /locus_tag="IV353_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9010139.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5039 a         3591 c         2981 g         5553 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gaca
//
LOCUS       DACDLI010000054        16615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDLI010000054 DACDLI010000000
VERSION     DACDLI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.054"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="IV353_001898"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="IV353_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9010140.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="IV353_001899"
     CDS             complement(680..1666)
                     /locus_tag="IV353_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9010141.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="IV353_001900"
     CDS             complement(1689..2255)
                     /locus_tag="IV353_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9010142.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="IV353_001901"
     CDS             complement(2269..2685)
                     /locus_tag="IV353_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9010143.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="IV353_001902"
     CDS             complement(3142..5076)
                     /locus_tag="IV353_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9010144.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5378..6313
                     /locus_tag="IV353_001903"
     CDS             5378..6313
                     /locus_tag="IV353_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9010145.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="IV353_001904"
     CDS             6403..7506
                     /locus_tag="IV353_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9010146.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="IV353_001905"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="IV353_001905"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9010147.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IV353_001906"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="IV353_001906"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9010148.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12128)
                     /locus_tag="IV353_001907"
     CDS             complement(10887..12128)
                     /locus_tag="IV353_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9010149.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="IV353_001908"
     CDS             complement(12146..13069)
                     /locus_tag="IV353_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9010150.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="IV353_001909"
     CDS             complement(13290..15782)
                     /locus_tag="IV353_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9010151.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="IV353_001910"
     CDS             complement(15789..16256)
                     /locus_tag="IV353_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9010152.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16392..16465)
                     /locus_tag="IV353_001911"
     tRNA            complement(16392..16465)
                     /locus_tag="IV353_001911"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16429..16431),aa:Arg,seq:acg)
     gene            complement(16523..16596)
                     /locus_tag="IV353_001912"
     tRNA            complement(16523..16596)
                     /locus_tag="IV353_001912"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16560..16562),aa:Pro,seq:tgg)
BASE COUNT         4907 a         3475 c         2958 g         5275 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg atggcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtcttg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttga tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    16441 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    16501 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16561 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DACDLI010000055        16144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDLI010000055 DACDLI010000000
VERSION     DACDLI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.055"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..656
                     /locus_tag="IV353_001913"
     CDS             <1..656
                     /locus_tag="IV353_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9010153.1"
                     /translation="KDRFSKWNKPVDRSEWEMSADTVNAYYHPFRNVIVFPAAILQAP
                     FYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTEEDLTQFQNKAQA
                     MIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSIEEFFVNWATIWR
                     TKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAMYLAPEKRVRIW"
     gene            complement(1197..4475)
                     /locus_tag="IV353_001914"
     CDS             complement(1197..4475)
                     /locus_tag="IV353_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9010154.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(4746..5291)
                     /locus_tag="IV353_001915"
     CDS             complement(4746..5291)
                     /locus_tag="IV353_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9010155.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(5303..5995)
                     /locus_tag="IV353_001916"
     CDS             complement(5303..5995)
                     /locus_tag="IV353_001916"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9010156.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(6014..6298)
                     /locus_tag="IV353_001917"
     CDS             complement(6014..6298)
                     /locus_tag="IV353_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010157.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(6311..6874)
                     /locus_tag="IV353_001918"
     CDS             complement(6311..6874)
                     /locus_tag="IV353_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9010158.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            7102..7764
                     /locus_tag="IV353_001919"
     CDS             7102..7764
                     /locus_tag="IV353_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9010159.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            7888..9084
                     /locus_tag="IV353_001920"
     CDS             7888..9084
                     /locus_tag="IV353_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9010160.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(9185..10561)
                     /locus_tag="IV353_001921"
     CDS             complement(9185..10561)
                     /locus_tag="IV353_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9010161.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(10598..11464)
                     /locus_tag="IV353_001922"
     CDS             complement(10598..11464)
                     /locus_tag="IV353_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9010162.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(11553..12578)
                     /locus_tag="IV353_001923"
     CDS             complement(11553..12578)
                     /locus_tag="IV353_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9010163.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(12596..13270)
                     /locus_tag="IV353_001924"
     CDS             complement(12596..13270)
                     /locus_tag="IV353_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9010164.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(13454..14347)
                     /locus_tag="IV353_001925"
     CDS             complement(13454..14347)
                     /locus_tag="IV353_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9010165.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQDTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(14421..16118)
                     /locus_tag="IV353_001926"
     CDS             complement(14421..16118)
                     /locus_tag="IV353_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9010166.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSVEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         4681 a         3489 c         2839 g         5135 t
ORIGIN      
        1 taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg gaaatgagtg
       61 ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt ccggcagcga
      121 tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat ggtggaatcg
      181 gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct ttatttgacg
      241 aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt caaaacaaag
      301 cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa gtaaacggaa
      361 aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca ttggaagcag
      421 caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca acgatttggc
      481 gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc catgcccctg
      541 ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa acatttggta
      601 tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc tggtaagagg
      661 ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat cgcttctcaa
      721 atttttgtga catttcggct tatttttcga agaagttact tctggaatac catgtattag
      781 aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa aatggctttc
      841 cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca ccgtttatcg
      901 agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga aaaatagttc
      961 ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac accgtttagt
     1021 ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaagcagc
     1081 aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg tctcatttcc
     1141 ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa ttccacttat
     1201 ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc tccagctaat
     1261 gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc atttttttca
     1321 ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc atttggtgat
     1381 tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc ggcagtttct
     1441 cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca tttttcccca
     1501 tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga aggtgctgaa
     1561 aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat ttggatcttt
     1621 tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt gaaagttgac
     1681 acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc cgccacacca
     1741 tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg atttggttct
     1801 tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc aaaaatagca
     1861 cctacagata atggaagacc atccactctt tccttttctg tttgacgatt ccccggttta
     1921 atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac aggctgctga
     1981 ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt atgtccaaga
     2041 gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg gtagatttga
     2101 tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc tgttcggaaa
     2161 gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata aagagccaca
     2221 ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt tccactattg
     2281 gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc atgagggccc
     2341 gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc aattcctggt
     2401 acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat cgaacctgtt
     2461 tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat acaaccgtca
     2521 aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc ttcaaatcct
     2581 ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg ccatgcttcg
     2641 aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg ataagcatga
     2701 atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat agctgcacta
     2761 tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat cccatttcca
     2821 gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc aatcaactga
     2881 atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct ttcgaaggaa
     2941 taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa tgcttgtgtc
     3001 agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg tgttacataa
     3061 actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt tgtttcataa
     3121 ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa actgaaagta
     3181 aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt cgcagtgata
     3241 gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat tgtcaataat
     3301 ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg attactagag
     3361 gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg aggatctgtc
     3421 gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt ccttgctgca
     3481 gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca gtcatacatg
     3541 atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc taaaccagta
     3601 ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg aaacaaattc
     3661 ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa ccctgctgaa
     3721 gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc ttgcggatct
     3781 tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt atatttgctg
     3841 ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat ccgactgcct
     3901 agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt tggtactgcg
     3961 tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga ttctatttcg
     4021 atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata ataggtcatt
     4081 ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag ccgcacgccg
     4141 tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa aacaacccct
     4201 ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc tgattctttc
     4261 atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg tacttcctcc
     4321 attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga ctgttgttcg
     4381 ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag gagattcatc
     4441 attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt aacaaattag
     4501 aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa acaaaattat
     4561 aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat aaagagattt
     4621 attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca taaaacaaag
     4681 tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca aatcactttg
     4741 tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc ccattcatgt
     4801 ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta tcctagatta
     4861 tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt caaataaata
     4921 tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa agcggaataa
     4981 tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta taaacttttt
     5041 aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg aaataagcgg
     5101 ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac gacaaaatcc
     5161 ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac ttgtcctgct
     5221 gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc aatcgagatc
     5281 aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc accatttctg
     5341 ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc gagtgatctg
     5401 ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga gctgtttgtc
     5461 caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt aagatttctt
     5521 tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct tcttttgcgt
     5581 tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg cttgcttcat
     5641 aatatagagg cattcctggt aattgtcctg gtttgtagcc aaatcctgtt gcatctgcat
     5701 caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct tcaccgattc
     5761 cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc aacagacttg
     5821 tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact tcgtggttac
     5881 ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt tcgcttaggt
     5941 tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt ttcatctttt
     6001 taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac attaggataa
     6061 caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc cgttcgctga
     6121 tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag ctggcaatct
     6181 ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat gactcgcttg
     6241 cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct ttggccataa
     6301 gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca taaatcgtct
     6361 ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga gttagatgat
     6421 acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc tctacccctt
     6481 gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac atcgaagtaa
     6541 gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta gctgctgtca
     6601 tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc aaaatcactt
     6661 tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg tcaaaagcaa
     6721 tgatcccgac accaccagga tgaaatacca gttctctctt cgaagttcct ccatctggca
     6781 aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct ttgcgactga
     6841 ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc tttgtaatca
     6901 taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt ttaccataga
     6961 taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac tctttctagt
     7021 agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat actcaacgat
     7081 aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct agcattggtc
     7141 ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact tttattgata
     7201 ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg aaaacaagaa
     7261 gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg tatgacagcg
     7321 aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat caatcaattg
     7381 caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca tgatccagtc
     7441 aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact gcatgaagca
     7501 agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa gtataatgag
     7561 atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga aagcgcgcag
     7621 atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt tgctgaggat
     7681 ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg tgataataaa
     7741 tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg gaacattcag
     7801 atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt aagaatcgag
     7861 aacagattca acaaggagga attttttatg gatcataaac cgactgaaac aaaagatgtt
     7921 acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac tccaacagat
     7981 acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca gacagctgct
     8041 cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct agcagcaaaa
     8101 atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca agcaaaatta
     8161 agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg tcctgtagga
     8221 gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga actgcgtgcc
     8281 ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat ctatgaagtg
     8341 acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa attagataaa
     8401 gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca gaaaaacaaa
     8461 gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat ggaagaactt
     8521 caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga tcagatggat
     8581 gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg tacgcatgat
     8641 cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt gatccaaaac
     8701 acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat tccgctgtgg
     8761 aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt aactgctcaa
     8821 cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat gctgaaaatc
     8881 tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga gacccttcaa
     8941 accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca aaaagaagga
     9001 aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga tctgaaacaa
     9061 aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca aacgcccaac
     9121 aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac ttctccattg
     9181 gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa atgatcagtc
     9241 caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga agcatcaata
     9301 caatacaagt caagatggag acgattggca atagcggaac gaatggcact ttgaattctc
     9361 cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta acaagcatca
     9421 aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg aaaaatccgg
     9481 caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta cgatgctttt
     9541 gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca aacagtaaac
     9601 gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt gcccctacag
     9661 cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc actgggtcat
     9721 tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg atgatgtaaa
     9781 ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt tgcgggtttt
     9841 tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa aacacaagag
     9901 ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag ttatcaggtt
     9961 taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg acatatacca
    10021 tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa gtgacgaata
    10081 tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg tacccgccag
    10141 ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata tagccagacc
    10201 atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac caccctgtca
    10261 gccaggcagc aaattcacca aatacaacat acagataggc atatggccca ccaatcactg
    10321 gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg attgcagcta
    10381 agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc cctgtcacca
    10441 caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca gacgttttta
    10501 gttctttttt catctcactc gtatgattga gattgatctc tttcttacga aacaaatcca
    10561 ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc atatacccta
    10621 aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg agctcttttt
    10681 tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg atccccattt
    10741 gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc atgcagatgt
    10801 ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga tcgactttaa
    10861 ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga ggtaagataa
    10921 ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca taaccaatcg
    10981 tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta tctgtatcta
    11041 tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga agagctaaag
    11101 caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa acggctcccg
    11161 caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca gaaacaccca
    11221 tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag ccggataaaa
    11281 atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag acaactaaag
    11341 aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg ccaccaattt
    11401 taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct attaaaattt
    11461 ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa acgttaacaa
    11521 cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata aaaatttaag
    11581 attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc cgtcaaatgg
    11641 atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt tgaatgtgtt
    11701 ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt caagtcttcc
    11761 gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc ttccttttct
    11821 atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact aagcaattgg
    11881 ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc accaacgatc
    11941 tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac aggctgaatg
    12001 attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc gagtcttgca
    12061 taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc tagtaatgta
    12121 tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga agcggaaagt
    12181 ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg ttgattactg
    12241 attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata ttcttctacc
    12301 agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga agttttttgt
    12361 atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag cagataaaaa
    12421 atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc aactttgatg
    12481 tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca tcccaagatc
    12541 aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca tcctttcagg
    12601 gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct gcctgttcga
    12661 tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca aatcgttcgt
    12721 catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg tgcttcatca
    12781 aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg ttgatttcca
    12841 tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca ctagataatt
    12901 ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt acatcgattt
    12961 ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc atatccatat
    13021 tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt attttttgac
    13081 accaataata gccatccaaa acgggaagat taatatctag taatacaagc tgtggttctt
    13141 ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt tcaaattgcc
    13201 atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg actaccatga
    13261 tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca aagaagccct
    13321 tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa aaaaactgga
    13381 aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta tgttattctt
    13441 aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta aattttcgat
    13501 aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt ataagtaatc
    13561 gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct gtaaagggaa
    13621 ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta caagtgataa
    13681 gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt ggaaggacaa
    13741 caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga tagaatcggt
    13801 catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata ccgctatggg
    13861 cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgtct tgcaggactg
    13921 tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag ctaacattga
    13981 ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt cctggatttt
    14041 ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct gggattactg
    14101 atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc tcatgttttt
    14161 cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga tccaaataat
    14221 tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca agaaacatca
    14281 ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta cctattttct
    14341 ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata aaaccgattg
    14401 ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg gtaccagctg
    14461 tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc gttcccacca
    14521 gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg attttctact
    14581 cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc agtaaaggct
    14641 gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat ttgataggtt
    14701 ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa ttctgttgct
    14761 tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc taaaggcgac
    14821 tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc gaatcttgca
    14881 tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc acctgttgtg
    14941 actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg tccgatttca
    15001 ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac cttcaaatta
    15061 tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt atctactttg
    15121 gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac tacttctact
    15181 atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat ttcctgatca
    15241 taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat aacagcgctt
    15301 ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt gtaagaagcc
    15361 gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc ttcattaata
    15421 atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc ttctctgatt
    15481 tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt agaaccatcc
    15541 cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat tggagacgcg
    15601 attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt atctctagaa
    15661 gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc ttcattcgtt
    15721 gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt gtgccgtttt
    15781 tgaatccatt caatcgcttc gacagaatta agaccgtctt ctcgttccgg tgttaaagga
    15841 ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa atcgactcct
    15901 ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg ttcttccgga
    15961 acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt ttgtgcgact
    16021 tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt aacgagtaac
    16081 agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc gttctttttt
    16141 tatt
//
LOCUS       DACDLI010000056        16065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDLI010000056 DACDLI010000000
VERSION     DACDLI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.056"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            289..606
                     /locus_tag="IV353_001927"
     CDS             289..606
                     /locus_tag="IV353_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010167.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="IV353_001928"
     CDS             complement(697..1062)
                     /locus_tag="IV353_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010168.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="IV353_001929"
     CDS             1270..1752
                     /locus_tag="IV353_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010169.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /gene="pstS"
                     /locus_tag="IV353_001930"
     CDS             1949..2839
                     /gene="pstS"
                     /locus_tag="IV353_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP9010170.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="IV353_001931"
     CDS             3165..5639
                     /locus_tag="IV353_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9010171.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="IV353_001932"
     CDS             complement(5742..7478)
                     /locus_tag="IV353_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9010172.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="IV353_001933"
     CDS             complement(7475..8179)
                     /locus_tag="IV353_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9010173.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="IV353_001934"
     CDS             8310..8813
                     /locus_tag="IV353_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9010174.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="IV353_001935"
     CDS             8773..9111
                     /locus_tag="IV353_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9010175.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="IV353_001936"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="IV353_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9010176.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="IV353_001937"
     CDS             10662..11240
                     /locus_tag="IV353_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9010177.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="IV353_001938"
     CDS             11244..11765
                     /locus_tag="IV353_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9010178.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="IV353_001939"
     CDS             complement(11844..12398)
                     /locus_tag="IV353_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9010179.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="IV353_001940"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="IV353_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9010180.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="IV353_001941"
     CDS             12938..13189
                     /locus_tag="IV353_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9010181.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="IV353_001942"
     CDS             13313..14104
                     /locus_tag="IV353_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010182.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="IV353_001943"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="IV353_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9010183.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="IV353_001944"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="IV353_001944"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9010184.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="IV353_001945"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="IV353_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9010185.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         5357 a         2861 c         3273 g         4574 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtatt
//
LOCUS       DACDLI010000057        16025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDLI010000057 DACDLI010000000
VERSION     DACDLI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.057"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IV353_001946"
     CDS             226..894
                     /locus_tag="IV353_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9010186.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IV353_001947"
     CDS             complement(1094..1480)
                     /locus_tag="IV353_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010187.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IV353_001948"
     CDS             complement(1452..2834)
                     /locus_tag="IV353_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9010188.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IV353_001949"
     CDS             complement(2990..4177)
                     /locus_tag="IV353_001949"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9010189.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IV353_001950"
     CDS             complement(4184..5215)
                     /locus_tag="IV353_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9010190.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IV353_001951"
     CDS             complement(5231..6685)
                     /locus_tag="IV353_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9010191.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IV353_001952"
     CDS             6860..7765
                     /locus_tag="IV353_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9010192.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IV353_001953"
     CDS             7856..8533
                     /locus_tag="IV353_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9010193.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IV353_001954"
     CDS             8814..9710
                     /locus_tag="IV353_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9010194.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IV353_001955"
     CDS             complement(9765..11135)
                     /locus_tag="IV353_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9010195.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IV353_001956"
     CDS             11373..12119
                     /locus_tag="IV353_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9010196.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IV353_001957"
     CDS             12116..12955
                     /locus_tag="IV353_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9010197.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IV353_001958"
     CDS             12956..13906
                     /locus_tag="IV353_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9010198.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IV353_001959"
     CDS             14047..14799
                     /locus_tag="IV353_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010199.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IV353_001960"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IV353_001960"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9010200.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5199 a         3006 c         3227 g         4593 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctgcattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactg
//
LOCUS       DACDLI010000058        15935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDLI010000058 DACDLI010000000
VERSION     DACDLI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.058"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IV353_001961"
     CDS             complement(59..187)
                     /locus_tag="IV353_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9010201.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV353_001962"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV353_001962"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9010202.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IV353_001963"
     CDS             complement(1674..2729)
                     /locus_tag="IV353_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9010203.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV353_001964"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV353_001964"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9010204.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV353_001965"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV353_001965"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9010205.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV353_001966"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV353_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9010206.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV353_001967"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV353_001967"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9010207.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV353_001968"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV353_001968"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9010208.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IV353_001969"
     CDS             10491..12050
                     /locus_tag="IV353_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9010209.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IV353_001970"
     CDS             complement(12161..14092)
                     /locus_tag="IV353_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9010210.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV353_001971"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV353_001971"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9010211.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IV353_001972"
     CDS             complement(15021..15773)
                     /locus_tag="IV353_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9010212.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         4121 a         3490 c         2916 g         5408 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 ttcagactgt agacaaaagc tgtctgtgag gacag
//
LOCUS       DACDLI010000059        15860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDLI010000059 DACDLI010000000
VERSION     DACDLI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.059"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..482)
                     /locus_tag="IV353_001973"
     CDS             complement(12..482)
                     /locus_tag="IV353_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9010213.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IV353_001974"
     CDS             complement(641..1099)
                     /locus_tag="IV353_001974"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9010214.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IV353_001975"
     CDS             complement(1270..2415)
                     /locus_tag="IV353_001975"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9010215.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IV353_001976"
     CDS             complement(2428..3345)
                     /locus_tag="IV353_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9010216.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IV353_001977"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IV353_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9010217.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="IV353_001978"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="IV353_001978"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9010218.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="IV353_001979"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="IV353_001979"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9010219.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="IV353_001980"
     CDS             6871..7752
                     /locus_tag="IV353_001980"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9010220.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="IV353_001981"
     CDS             7749..8465
                     /locus_tag="IV353_001981"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9010221.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="IV353_001982"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="IV353_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9010222.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="IV353_001983"
     CDS             9182..10183
                     /locus_tag="IV353_001983"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9010223.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="IV353_001984"
     CDS             10180..11541
                     /locus_tag="IV353_001984"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9010224.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="IV353_001985"
     CDS             11501..12652
                     /locus_tag="IV353_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9010225.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="IV353_001986"
     CDS             12627..13796
                     /locus_tag="IV353_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9010226.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="IV353_001987"
     CDS             13971..15350
                     /locus_tag="IV353_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9010227.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..15860
                     /locus_tag="IV353_001988"
                     /pseudo
     CDS             15631..15860
                     /locus_tag="IV353_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5184 a         2800 c         3379 g         4497 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctaacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaagaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tacagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcatacag ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg gggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccgc aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt
//
LOCUS       DACDLI010000060        15186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDLI010000060 DACDLI010000000
VERSION     DACDLI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.060"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            164..358
                     /locus_tag="IV353_001989"
     CDS             164..358
                     /locus_tag="IV353_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010228.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            627..926
                     /locus_tag="IV353_001990"
     CDS             627..926
                     /locus_tag="IV353_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010229.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1023..1457)
                     /locus_tag="IV353_001991"
     CDS             complement(1023..1457)
                     /locus_tag="IV353_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9010230.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1689..2399)
                     /locus_tag="IV353_001992"
     CDS             complement(1689..2399)
                     /locus_tag="IV353_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9010231.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2389..3249)
                     /locus_tag="IV353_001993"
     CDS             complement(2389..3249)
                     /locus_tag="IV353_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9010232.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3254..3892)
                     /locus_tag="IV353_001994"
     CDS             complement(3254..3892)
                     /locus_tag="IV353_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9010233.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3907..4206)
                     /locus_tag="IV353_001995"
     CDS             complement(3907..4206)
                     /locus_tag="IV353_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9010234.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(4249..5733)
                     /locus_tag="IV353_001996"
     CDS             complement(4249..5733)
                     /locus_tag="IV353_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9010235.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5753..6214)
                     /locus_tag="IV353_001997"
     CDS             complement(5753..6214)
                     /locus_tag="IV353_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9010236.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6232..7299)
                     /gene="ulaG"
                     /locus_tag="IV353_001998"
     CDS             complement(6232..7299)
                     /gene="ulaG"
                     /locus_tag="IV353_001998"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9010237.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7517..8287
                     /locus_tag="IV353_001999"
     CDS             7517..8287
                     /locus_tag="IV353_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9010238.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8560..9387)
                     /locus_tag="IV353_002000"
     CDS             complement(8560..9387)
                     /locus_tag="IV353_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9010239.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9954..11189
                     /locus_tag="IV353_002001"
     CDS             9954..11189
                     /locus_tag="IV353_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9010240.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12188..12448)
                     /locus_tag="IV353_002002"
     CDS             complement(12188..12448)
                     /locus_tag="IV353_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9010241.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12475..13776)
                     /locus_tag="IV353_002003"
     CDS             complement(12475..13776)
                     /locus_tag="IV353_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9010242.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14068..14526)
                     /locus_tag="IV353_002004"
     CDS             complement(14068..14526)
                     /locus_tag="IV353_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9010243.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14539..>15186)
                     /locus_tag="IV353_002005"
     CDS             complement(14539..>15186)
                     /locus_tag="IV353_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9010244.1"
                     /translation="KEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYP
                     DMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAIS
                     LLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVETITLRKPISDFHKGLMEA
                     QVAAEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
BASE COUNT         4856 a         3095 c         2363 g         4872 t
ORIGIN      
        1 aacaatagcc ttttattata tatattattc aaattagtta tatcgagtga aaaaaacttt
       61 caaattgatt ctttttattt atcataaaga tatagataca taatcatttt cacttacata
      121 tatttgaata ttaactaaaa acagcaagaa agaagaggat caaatgtatt atgtagaagt
      181 aaaaacaaaa ggcgtcaaaa acaaacaata tgtgaaaggt atcagtaacg aatatccttt
      241 acttggttca tggaaagaag ctgctccttt ttccaaacca tgtgccataa aaataaagaa
      301 cgagctagaa aaagaactga cttgtggcaa agctgttgta gatatcatcg aaaagtaaat
      361 ccaatgtata gaaaggaaga tcacacatga aaagcgacgt acaaattgct aagattatag
      421 aattagaaga agaagacgca gtgttgacaa ttgaaaaaaa ataaatgttt gtataccata
      481 tgattatttc aatatgtatc ctggaattgg agatctcgta gagctatatc aagatgaatc
      541 aaatatactt gtcattcatc atgaaatata gagacttata aaattgttta tttttataat
      601 gtatcagtat agaaaggaaa taaaacatgg attatactgt tgaatttgac agtgaactac
      661 ttaagcaatt tccacaagaa gaacatttca tgtataaaga agcaatttcc cacaaagtga
      721 cagagtattt ggagcataat ttttttcgta taaagccagt gcgtactcgt tcggcaaaag
      781 gaatatacga gatgaaagtc aaaattaata aaaaaaatta tcgcatagca tttgctttaa
      841 aaaaaaatca tgtgttcatt ttttatatta gttcgaattt gcaaaaaatt gtatttgata
      901 aagaggtgag taaaaaaata aaatagctga gcattggagg atagattgag tgatactaag
      961 tctatcctct tgtttttcta aagaactaag ctagaatttt ttcttttgta cgttattata
     1021 tgttatttgt cattaccaaa aagcaccttt tttcttctag tatctatgac ataagaaata
     1081 ccacccattt cctttacttg aacaaatcct actgattcat aaaatttttt tgttttctca
     1141 gtatcatctg tcaataattc gatttggact ggtccataac tagtgatctt ctccaaagct
     1201 tcattcaaaa gcactttgcc tattccttgg cgctgatacc tcggatgaat gaggatatct
     1261 tgaataaatg cgatcgtcgc aaaatcagtg atccaacgaa cgattccgat aatcttccct
     1321 tccccattac gctttatcag agattcagat tgttcaaatg ctctagctaa ccttgcagtg
     1381 tcttttgtgt aattcgtcca accaacgctc ttatataatt cagaaatctc atcgatcata
     1441 agggaatcaa tattcatttt tttcttcctt tcttatacaa ttctccctct aataatacag
     1501 ttctttgact atttaatcta tcacatataa ctctcctgta aagattctct cgattcctta
     1561 aaatctctct ttccttttag aatgcatcct ttttgataaa taaaaaggtt gtgataaatt
     1621 ttcttcacaa cctctgtttc tctctatcaa gtgttatctt ttaattttct acccttaccc
     1681 tagcatctct attgcccgta gtatgcatac tttccatgtt tgcgaaaata atgtttatct
     1741 agtaaatatt gcgggatttc acatatattg gggttgatca gctcgttttc tttggccatc
     1801 agacaaattt catctaagat caaactgttt tctactgctt tcataggtga atccccccat
     1861 gtgaacggtc catgtccata gacaagtact ccagggactt cgtttggatc taattttctt
     1921 tccttaaacg tctcgacaat cacattccct gtatgaactt cgtaagcttc tcttacttct
     1981 tctttcgtta attggcgtgt acaaggaacc ttaccataaa aagcatcagc atgagtcgtg
     2041 ccataagctg gcaaatcgcg tcctgcttgc gcccacatga cggagtgcgt ggaatgagta
     2101 tgagtaatag cagttatatc ttcaaaagtt tgatacaaaa caacatgtgt ggggagatct
     2161 gaagatggtt ttaagctatc ttcttctatt atatttcccg acaaatctgt taccaccatt
     2221 tgatctgctt gcatgcattc atatctgaca ccgcttggct taatgacgat gactcctagc
     2281 tcacggtcca cttcactcac attgccccaa gtcaatttga ctaatccgta tcgcggcaat
     2341 gccagatttg cttcaaggac acgttgcttc attgctgcaa ttttttcttc acaagacatc
     2401 atagccagcc tccttcaatt tagaacttaa gtagtctttt gcctgctgga tttctcttcg
     2461 aaagtcttgg cttttttcac tccacatttc aatcagaaat gttccatcat aatctagacg
     2521 tttcaaatgt cttaataatc caagaaaatc aacacaaccg ctaccaaaag gaacatcacg
     2581 aaactggcct gaatattctt ttgttacagg gtaagtatct tttaagtgga tagcagtgat
     2641 ataatcgatc cctttctcaa gttctcgagc cggattattt tccggccatg cggacaaatt
     2701 gcctaggtct ggatatactt gtagaaaagg ggaatgaatc tgttctttga tttctaaaaa
     2761 ttttgaaatg gagttcataa atggatcatc catgatttcc acggaaagaa tgatgccata
     2821 tttagaagct tcctttacgc tttcttttat tccttcgata aagtcttctc tggaattcat
     2881 tgatttctct tcataataaa catcgtatcc agctatttgg attactttga cgcttagatt
     2941 atgcgccaaa tggattgctt ttttcattag ttgtttcgct gcttttttct tttctggatc
     3001 agcagaacca aacggaaatc gccgatgcgc acttaaacaa atcgaatgga tacgtacacc
     3061 ggagctgaac atctcttctc tcagctcagc tatcttttct tcagaccaat ctaatcgtgc
     3121 taaccgctca tccgtctcat caatcgacat ctcgataaaa tcgaagtcca tttcttttac
     3181 aagggaaaaa cgttctttcc atgaaatatc tttgggtaat gctttttcat aaatcccaat
     3241 ttgagccatt tccttacccc cagatacgaa tgatctcatc tttgaagttt tgtgcggcct
     3301 tcatcggatc atttgctgct gtaataccac gaccagtgat aaaggtatac acatcgatcc
     3361 cttcaaataa gtgtaatgtg tgtggattta atccaccagt tacagaaaca ttaaagccta
     3421 gttcaatcag ttttttgatc ttcgataaat ctttttcacc ccaagtttca ccagataaca
     3481 gcgcatcacg gctttgatgg tagattacct gccggatacc gatgttatgc caatccatgg
     3541 cttgttcaaa agtccaattg ccgtagagtt ctacttgcaa ttcacctatt tctttttgtg
     3601 ctgcctccat agttggaatc gttgcacaac aaatgactgt catccagtct gctcctgctt
     3661 ttgcaacatt actcgccact gttcctccag cgtctgcaca tttcgtatca gcgactagtt
     3721 ttttatctgg gaacatactg cgaatacagc gaatcgcttt ttgtccttcc tgtaagcaaa
     3781 gaattgtgcc cacttcaata atatctacta catcaccaac tttcataaca tctcctaatg
     3841 catgttctaa gtcattgtga tctaacgcaa tttgcaaatt cggcctgctc atcttagttc
     3901 cttcctttac ttgttttttt ggtaccactt tgtttcagca aatttttcac tgatttcttt
     3961 ggctgacatc acatttttga taccgataac tgtaacttct tttttctctg cctgagtaaa
     4021 catattcaaa aaattgactg gcgtaaaaac tacatcgtat tggccagctg aactttttcc
     4081 ctctgaaatg gcacaatgat ggattttggt aattggtacg tttagttctt tcattgcttt
     4141 ttccacactt ctcatcatca tcaagcttgt tcctgatcca tttgcacacg atactaaaat
     4201 tctcatctta atccctccat tatgagtgtt gtctaatcag attttctttt atactctctg
     4261 cttttctgca tatgtgtcat agtcttctac aatcaaaaaa taaccctcag gatttttccg
     4321 atattgtaac tgcggaatag ccaataaacc aatgacaact acggcaattc cagcataacc
     4381 taggactttc atgattactg taaacaatgg ccaagctgtt gcccagtcga acatcccgat
     4441 ataaccacca tatgttgaca tcccaatcca tgtagcgaat aatgctgaac cgcctacttg
     4501 tatgattcct gaaagaaacg ggaacaggca agcggctttg aatccacctc ggttattggc
     4561 ataaactgct ataacggcat tatcaaagaa caacggaatg aaaccggcta tgacgatcgt
     4621 tggtgatttc aataaaatca gtaaaccaat catcaaaaac tgtcccaaag caccaaacaa
     4681 gaacccaatc gttaccgcat ttggcgaacc aaagccaaaa gtagcagcta cgtcaattcc
     4741 tggaacagct cctggtaaaa ttgtgtttga gattccttgg aaagattgag ttagttcatc
     4801 gacgaatgtc cgaacaccta attgcaagat cgccagatat accgcaaaat ttaaagaagt
     4861 cgtcataatg tagaataaga aactctgtcc ttcttgcatg aattgcgctt gaatcaaata
     4921 atctttgcct agtacaatca gaatgatccc aaagaaaaat agcatgagta acgaagtagc
     4981 aaccatattc tcattgaaaa tcgacaagaa tcctggtaat ttgatatctt cgattttttt
     5041 gtcttgcttg attgatctat ttttcttttc atcccgcttt ttcatccact cagctaattt
     5101 tgcaaatata aagattccga acatttgctg atgtgccact gcaaatccgg caccttctgt
     5161 caattcctgc gtgattccta ctgttaagtt agaagctact gcccaataac aacctaaaat
     5221 cagtcccata aaaatcaata cttgtatcct acctagatca ggaaagcaaa acagtaaaat
     5281 ccaaaaagcg gtagctgctt gttgaacctg aacgtttccg gtagtgaaaa cggaacgcag
     5341 tttcgtatat ttctgaaaac ggactaataa gatatttaaa acaaatgcaa tcagtaataa
     5401 aatcatcaca tctccaaagg tacggccaaa tgtctccatt aatccagcat ccacagcatt
     5461 ttgtccaaaa taaggatctg taaccatggc atccaaattg aaccgttcct ttagtccaac
     5521 caaaatcggc cgaaaattat tgaccaaacc accagagcca acagtcaaaa taaagtaacc
     5581 gaccgttgcc tttaagaatc cagcgatgca ttcatgaagc gggcgtttca gcaaaatata
     5641 gcctaataac acgataaaac caatcaggta tgctggctgc gtcaagatat ttgttgcaaa
     5701 atacgtccaa atgcttaaca aaaattcccc catctttttt ctcctctatc aattaattat
     5761 attctgccat cacaacttgg taatcatcga tggagttcgt agagatgaga cgctcaacca
     5821 atccatctgt cattaataat tcggataatt tttggatatt ttctagatgt tcttctggat
     5881 cttttgctgc aagtgtaaaa aacaaactag ccgttttctc ctcatcctcc ccttcacttt
     5941 caaaacggat tggctgtttc aacttggtaa atgaaatagc tgttccaaaa acaccatcac
     6001 ttttttctga agaatgcggc attgctactt gaggtgcgat cacaatataa ggaccgtatt
     6061 tttctacaca atggatgatc tcctccacat acgtttcatc aataatttcc ttatcgatca
     6121 atacaagaca gctttcggcg atagcttcac gccaatccgt gatttcttta tctgggtaat
     6181 gaatcaattg atggtcaaaa aagtatttca gcaagaaact ccccttcttt cttataaaaa
     6241 cgacggaaac ggtgtatcat tttcaatcga gaagacatct tcaaatcctc gataaaaatt
     6301 aaactcaaga tcatctttgt ttgtcggata gatgaatttc ccgcctactt gccagatata
     6361 aggtttaaat ccatacttca ggcgatcctt tttgaatttc caaatttcag tgatctcttt
     6421 tgaatcagcc ataaaattag accaaatatc gtagtggaca ggaatcacta cttttgcatt
     6481 caatgactcc gccatgcgca gcatatcaac agaagtaact ttgtctgtta tccctcgtgg
     6541 gttttcacca taggccccta aacagacatc gattttgtgt tcattaccat gtttggcaaa
     6601 taaattggag taatgtgaat ctcctgcatg ataaagattc cctcctgatg tttcgaataa
     6661 atagtttact gcaatttgat ccatctcctg aggcattttt ccttttaata cctcttctgg
     6721 atcttcacaa gtgaccaacg ctgtccgatc aaacgcttct aaagctacta cattgatgtc
     6781 tttgattttg actgcatcac ctggctttac tgtaatcgtt ttttcttcag gaattcccca
     6841 tttttgccac gtgcgaacca cttcttttgg cccaatgaat gttgcttcag gacaattttg
     6901 atgaactgct gcggctgtat ggatatctag atgatcggaa tggatatgtg taacgattaa
     6961 agcgtctaca ttttttatcg caaaaggatc gattacgaaa ggctgagtac gtaaattagg
     7021 ctgcatattt tgaacgccac tcatacgcat catctgatgg ccttttttca tcatgccatt
     7081 cccatgacta cgttttcccg tcccacacca tagatcacat aaaatattcg ttccttcatg
     7141 acttttcagc caaattcccg tacagcccag ccaccacata gcaaaagttc caggatccac
     7201 tacttctgtt tcgatttctt cattcaagta tgtgccccac tcaggaaatg tttctactaa
     7261 ccacttttct tttgttactt cattgacagt tttactcatt tgcttgctcc ccccatatcg
     7321 ttgttccaac ttacaagtaa atattaatgt gtatcagcat ccatggaaac ccttttatat
     7381 gaccgtttgt tttttatatg attttctttc agtaatcaat gattgtttca gtataataaa
     7441 gacagatatg aatgtttaaa tgaacaacta gtcaaataaa tccatggatc aagatgtccc
     7501 tcaggaggca aaagtcatga aaaactcact tgtcaatatt gaaaaacgtc aaaaagaaat
     7561 tttggaactg cttcagcaga ctgaacaact atcaactttc gaattagctg aatcccttga
     7621 tgtatctatt agtacgatac gaagagattt aaatttatta gaagaaaaaa acgagatcat
     7681 acgcaattac ggttattgtt cttataatta caaaaaccag actgattttg atcaatctgg
     7741 tcctgaaagg attaaacaag cgattgccag agaagctagt aaatacatta acgattatga
     7801 tacgctgttt atcaattcta gctcgactgc gttgaaaaca ctggattttc ttcaatctaa
     7861 ccatttgaca gtcattacaa acaacgtgaa aatcaattac tctccgcaca aagataattg
     7921 tagttatatt ttaactggtg gagagatgcg ctttccaaaa gaagcactag tgggcgacat
     7981 cgcattaaat acgatttctt cagtcaatgc agacatttgt atcattggct gtagcggagt
     8041 cgatgtagta catggggtca caacaaagat attaaatgaa tcaacgatca acgaaatgat
     8101 ggtcaacaag acaatcaaat gtaagatttt agtcgctgat catcgaaaaa tcggactgac
     8161 atcaaagttc aagattgctg atatttcagt ctttgattat ttgatcactg ataaattttg
     8221 ttctgaaaaa gtattagagg agatcagaaa tacggggata aaaaccattc aagtagattc
     8281 attttaagaa ccaggacgta aatatgtaaa taggattatc aaaaaaatga ataacaaatt
     8341 tatcgataaa tgaaaaacac gctacgctca aattgataaa ggaatcccaa gatgtgtcca
     8401 tccaatcgat accatcataa tctattgctt cttttataac cgttatatgt tgcctaaata
     8461 ggaatcaaac aatatcgaaa aaaggaggta tgacaaaagt cacacctcct tttttcgaat
     8521 aaacggtatt caaatcagaa cactgatttc acaaccactt tatttcaggt cgttttcaat
     8581 cacctttacc gcgtaatcat ttccgttgaa acgcgctatt ttttttgcgt ccatatatct
     8641 ttctaaataa tcatcttctc ctaattgctt atggcaagaa gcaatttcat aaagcaagta
     8701 ttccaatgca taagtcgatt tatgtttctt attatactca atacccttct cacataattg
     8761 gattgcttta tcataatcct cgattttacg gtaataggtg gcgatatcat aatatacaat
     8821 gcagaccatc agattatcaa aatcagtctc actcaatatt ttaagtgtac gctccacata
     8881 aactttcgca aaatctaatt ctccttttga caagtagatc attgccacgt ttgcagctgc
     8941 ttgtaagtga tggatatctg tttcggtcgt ttgttccaac acttgattaa aataaaataa
     9001 tgctgcatct ggttttttcg cataaagatg atcaagcagt ccttgataaa ataaaatttg
     9061 atttaaaaga tcagaattac ttactttttc ctgatcgatc gtttgtaaaa ttttataaga
     9121 ttcttggtat tttgatacag aattcaatga ttgaatctca tttatttttt tcctgatttt
     9181 gtcttcttct gtttctacta atatatcgga gatctcgaca ttcagctttt tgcaaagcgc
     9241 acttaagata ttgatgcctg gcgacatatt cttgttttcg atattgctga cagttgcttg
     9301 ttcacaaatg ccttctgcta attgtacttg tgttaacccc tttttctttc ggtattcttt
     9361 tagtaatgct ccatctactt taatcaaaac aatttccttc cctttaaaaa attatttctc
     9421 ctttatatca tacacaaaaa aaagttctaa acaaccaaaa atgtttagaa cagaataaaa
     9481 attttaattt taaaataata ttcaaattaa acaactggcg tatctgctcc ttgatttctt
     9541 tttgtgttat ctgaataatt atcgtttaca gttataacac atagtgaaaa ttgtcatggc
     9601 gagaaagtct aagaatattt ttaaacataa ctatttcttt cttacgtgtg tataatgacg
     9661 acagcacacc tatcaattaa tataattatt ttatttttta tgttctcatg tttattttta
     9721 atgacacaac ttctcgtatt tatctggcac aacatataaa tcgatcaaaa aattgttgcg
     9781 tgtttttttg aaaattggag tttcaacttg attacaaaat ttaaatttag aagttgtgaa
     9841 aacagcgaga gacagtttaa tattcaaaat atacataggc tactcgaacg ctgtgttttt
     9901 tagtatacta gtaagaaacg aagggataga ctcattccaa taaggaggat ttcatgaaga
     9961 caattctttt tgctcctgca gtattcaatt tagctgagac aacacgaatg attgaggttg
    10021 ccaaagctat gaaagaccgt tattactgtg tattctttgg cttttctaaa acttttgctc
    10081 ctttgatcac agaaagtggc tttgagtatc atttactagc gccgatcctt acgaaaaaac
    10141 aagaagaaca aatcataaaa tttgaccagt tgcgtacact taaaaaccct tttaccaaag
    10201 aaatggttgc ctctcgtatc actagtgaac agagtctgat tgctaagttt gagcctcgac
    10261 tgattgtgac aggttcgaat gtgacaattt ttttatcggc ccgcaataaa aagattcctt
    10321 tagtatatgt taaaccctat gctttgagtg aacctttttt tcaatcagag catgcgctga
    10381 tgccccacga gttacgtttc ttcggccctt taagcaatcc cctttggcac tggacaaaaa
    10441 agcgtatagt aaatacccga tggatcccca atgccttcaa acacgctgca caaaaagaaa
    10501 tgcttccttt accaaaaaca agtttacaaa tgatggatgc tgatctaaat ctcatcacaa
    10561 cgcctaaact atttttggaa gaagaacagc tgccggaaaa ttatcgaatc gttggtccaa
    10621 tttttgctaa gctcagtact tccttgcctg aagaagtgct aacttttata cagcgaaagc
    10681 aacaagaaag aaaacgtatt atctatttcg caatgggtag ttcaggcaat ccgcgtatgt
    10741 tacgcaaaat tttgttcttc ttacaaaaac aaccggatct agcggttatt gcacctattg
    10801 actatctgct tgatgacatt caatctgact cagaagatct tttaataaca gaatatctgt
    10861 ctagtcatct tttgcatgaa ttcattgatt tttcttttat ccacggcggg gaagggaccg
    10921 tccaaactgc ctgtgaatcc ggcaaaccgt ttatcggtgt ggggatgcaa tttgagcaaa
    10981 gctgtaatat tgactattgt gtaaaatacg gaaacgcaat cgccctcaca aaaccagtga
    11041 ctgaaagaaa actgcagatt gctctgcgcg aagtttcttc acccaagatt cgtcagcagg
    11101 cacgcacgct gcaagccgtt tttccaataa atggtccaca acaagcaatt caagaaatcg
    11161 aaaccttcct gagcaaccac ccaatatagt ttcaagtaga aaaagctttt ttaatagaga
    11221 ctcatcagta atttcgctat tcgagaccca gacagtgaaa atacaaaaaa agtaaatagt
    11281 ggattgaaaa gttagagtta cttaaattac ggtgtctaaa aaacacaaaa tcatccttaa
    11341 aataaaagat ctgacgcaat ccaaatgagc ataggtagcg tctgccattt attcttagtc
    11401 aaattctttg acacctttct ctggttggat catctcaacg tttcttcaaa agaaagacca
    11461 taataattag gcaaatacat tttcttaaaa tgtgtcatat agatctagaa gcatcgcttt
    11521 gtagagattt gtgctgctag tcccttcaac aattactaaa tcaaatgtag gatttaccac
    11581 tttatctttg ataatcaacc tccctcttct gttgagacaa tgatctcgca atccgatgca
    11641 tactcctata taaactgcca gcaatcccca catgctgagt agatcatgtc agaatgttct
    11701 atgtcgtcag caacgataaa ctaaaagtca cgttcaccat gactgattgc tattcccagc
    11761 gcaattattt ctatacacgt ttcatgattt gaataaacac tctcatcata catattacca
    11821 ttcatacacc atactgcagc ccctactata tggttgtaag ctcccaaatc tatatttgcc
    11881 ttgatcactt tggtagtaga tcgactaatt cttaatccat tgctgataaa attttcattt
    11941 ttgcctcctt ctttctgcca aaacagttaa aacaataata atctttatgt aaaaaagggc
    12001 tgtgacaaaa gccttgtcac agtcccatat tccgaataaa cggtggtcaa aagcttgcat
    12061 ctgcggtaat aagttcaacc aaacgaaaaa taagccaaca tttcacaaaa atttaagaag
    12121 caattttcgt aaatttcttc ttatttcttg aagctaacca cttctgtcac aaccactttt
    12181 agaacattta aagcttcgat gtgacaaacc atctatgctg ctcgatcata aaattgatag
    12241 caaaaattcc acaaagtaca tatgtccccc atgaaaacaa aacattcata tgcggtacaa
    12301 tgatctttgg aagaatcacg atcaaaaatg atgccagtgc ccaaatccat cgattgattt
    12361 gaatatgata tgtagctagc atattcaata taaggtgact agccgttaat aaaaagataa
    12421 aaacaaaaaa taaaatcagc caatacattg tttcccacct tctaaaaacc aaacttagac
    12481 agtttggact tcgttttctc cacgactttg ttcatctgac agcatctttt tatcataagc
    12541 ttttacaaat ggataataga taataaatgc tatcaatgtg ttgatcatga tcaagataac
    12601 aacacgccag tcatttcctg ctgaaagata accgccgatt ggagctggta atgtccaagt
    12661 tggcaaaaca gtaaatctat tgaccatttc taatttgact gcaaaataac tgataatagt
    12721 caaaactaaa ggaactaagt tgaacgggat gaacatgtaa ggattcaaca tcataggagc
    12781 tccaaaaatc atgggctcat tgatattgaa gataccagga ataatagaaa aacgaccaac
    12841 ctctttcaag aattttgatt tacagaagaa tataaacaaa acagatacca cgatcgttgc
    12901 tcctgcacca ccaattgtta ccgtccattg ataaaactgt tcaggagcga tatagggtaa
    12961 ttgggtggca gctgtaccat ctgccaatgc ctttgcattc tcatccaaca tgatcagcca
    13021 cattggacga acaacagccc ctacaatact catgccatgg ataccaaatg accacaacaa
    13081 atggatcaaa aacattggta gtaaaacacc aagcaaatta ttccctaaaa tattggttaa
    13141 aggctcgaat aacgataata aggtagcgtt tacatcaaag cccgcgacgc ttcgaatcag
    13201 ccaagtaatt gttaaaatga ctgcacctgg aatcaatgcc tcaaaactac gggcaacagc
    13261 aggtggcact tgttctggca ttttgatagt aatatttcga tttttaaaga aacgatatac
    13321 ttccactgca aaaatcatag ataagatccc cgcaaacatc ccagatgctc ccaaagaact
    13381 cataggtaag acccagccaa gaggtgcctt ttcaggagtt aaaacgttta caggaacttg
    13441 taacataaag aacgttccta aagataatat accacctgtc acactatcta atccatatga
    13501 tttagctaga tccgaaccca taacaaaagt tgcataaata gccatcaatc ccatcgtcat
    13561 cctaaaaggt aaaacaatcg ttgccgaata aggggcaata aattcattcc atccaggaaa
    13621 cggtaaattg ataaagacca taaaaaatgt cccaatcaaa atcaatgaca atgttgaaac
    13681 aaccccatta cgaattgcta ataaatgacg ctgtgtacca atttttgcca ttggtgggac
    13741 aagtttctct tcgacccatt tcatcagctt ttccactaaa accactcctt ttcaataaaa
    13801 tatgaaccat tcattgcatg acaacgcaaa gaatgttcat aactaattat ataattaaga
    13861 aattttattt caatatattc tcttaaataa tcaaatttat ttttaagaat cctgaaaata
    13921 agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat caaaatgatt
    13981 aaatctagtg attgaaactt atcatttcaa aaaaattttg taaattcacc cataaatatc
    14041 accctattta acttttttca tcgtacacta aaattcaaaa caactatctt taatctttac
    14101 ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta caatgatcac
    14161 cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca gtgtcccttc
    14221 ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga gttcaactgt
    14281 atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg ttatggcatg
    14341 tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat atccatcatg
    14401 attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt cttttactgc
    14461 tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg ttttaaagaa
    14521 atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta ctttgatcat
    14581 ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca atgtaaatgc
    14641 ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact tctctgctgc
    14701 aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta gtgtaattgt
    14761 ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg accgtagatt
    14821 cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat cattcaacaa
    14881 ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt tcaatacttc
    14941 ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct ctcgtccatc
    15001 catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca tatcaggata
    15061 taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg tgatgactga
    15121 catcatttga aaggtttttt gccaagtttt gtctcctata ttaaaatcgg cgacttgcat
    15181 ttcttt
//
LOCUS       DACDLI010000061        14879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDLI010000061 DACDLI010000000
VERSION     DACDLI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.061"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..506
                     /locus_tag="IV353_002006"
     CDS             132..506
                     /locus_tag="IV353_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9010245.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="IV353_002007"
     CDS             complement(620..1249)
                     /locus_tag="IV353_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9010246.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="IV353_002008"
     tRNA            complement(1383..1455)
                     /locus_tag="IV353_002008"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
     gene            1669..1742
                     /locus_tag="IV353_002009"
     tRNA            1669..1742
                     /locus_tag="IV353_002009"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1703..1705,aa:Asn,seq:gtt)
     gene            1697..1846
                     /locus_tag="IV353_002010"
     CDS             1697..1846
                     /locus_tag="IV353_002010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010247.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2339..3667
                     /locus_tag="IV353_002011"
     CDS             2339..3667
                     /locus_tag="IV353_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9010248.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3869..4702
                     /locus_tag="IV353_002012"
     CDS             3869..4702
                     /locus_tag="IV353_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9010249.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4718..5455
                     /locus_tag="IV353_002013"
     CDS             4718..5455
                     /locus_tag="IV353_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9010250.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5610..6578)
                     /locus_tag="IV353_002014"
     CDS             complement(5610..6578)
                     /locus_tag="IV353_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9010251.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="IV353_002015"
     CDS             6810..7643
                     /locus_tag="IV353_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9010252.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="IV353_002016"
     CDS             7677..8516
                     /locus_tag="IV353_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9010253.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="IV353_002017"
     CDS             8542..9102
                     /locus_tag="IV353_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9010254.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="IV353_002018"
     CDS             9301..11022
                     /locus_tag="IV353_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9010255.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11187..12329
                     /locus_tag="IV353_002019"
     CDS             11187..12329
                     /locus_tag="IV353_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9010256.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12345..13556
                     /gene="thiI"
                     /locus_tag="IV353_002020"
     CDS             12345..13556
                     /gene="thiI"
                     /locus_tag="IV353_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9010257.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14129..14776
                     /locus_tag="IV353_002021"
     CDS             14129..14776
                     /locus_tag="IV353_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9010258.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
BASE COUNT         5070 a         2477 c         3008 g         4324 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tacttgattc cggcatagct
     1681 cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactgccgg
     1741 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
     1801 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
     1861 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
     1921 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
     1981 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
     2041 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
     2101 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
     2161 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
     2221 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
     2281 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
     2341 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
     2401 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
     2461 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
     2521 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
     2581 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
     2641 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
     2701 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     2761 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     2821 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     2881 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     2941 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     3001 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     3061 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     3121 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     3181 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     3241 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     3301 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     3361 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     3421 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     3481 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     3541 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     3601 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     3661 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     3721 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     3781 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     3841 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     3901 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     3961 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     4021 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     4081 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     4141 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     4201 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     4261 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     4321 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     4381 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     4441 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     4501 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     4561 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     4621 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     4681 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     4741 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     4801 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     4861 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     4921 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     4981 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     5041 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     5101 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     5161 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     5221 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     5281 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     5341 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     5401 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     5461 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     5521 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     5581 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     5641 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     5701 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     5761 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     5821 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     5881 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     5941 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     6001 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     6061 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     6121 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     6181 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     6241 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     6301 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     6361 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     6421 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     6481 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     6541 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
    11041 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
    11101 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
    11161 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
    11221 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
    11281 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
    11341 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
    11401 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
    11461 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
    11521 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
    11581 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
    11641 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    11701 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    11761 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    11821 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    11881 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    11941 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    12001 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    12061 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    12121 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    12181 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    12241 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    12301 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    12361 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    12421 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    12481 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    12541 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    12601 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    12661 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    12721 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    12781 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    12841 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    12901 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    12961 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    13021 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    13081 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    13141 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    13201 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    13261 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    13321 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    13381 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    13441 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    13501 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    13561 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    13621 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    13681 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    13741 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    13801 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    13861 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttgcg
    13921 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
    13981 acgtttggtt gagcttatta ccgcagatgc aagcttttga acaccgtttt atttgtctca
    14041 cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt acactaaata
    14101 tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc
    14161 aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg actcaggtat
    14221 aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg caacgatccg
    14281 cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg atgtcccttt
    14341 tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa tcgcattagt
    14401 aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa atgaaaatct
    14461 aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa ttgatggaga
    14521 aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc
    14581 aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg tcaaaggaaa
    14641 tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt atgtcaatgt
    14701 tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg agaattactc
    14761 agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa
    14821 ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac ttgttgact
//
LOCUS       DACDLI010000062        14602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDLI010000062 DACDLI010000000
VERSION     DACDLI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.062"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IV353_002022"
     CDS             complement(<1..430)
                     /locus_tag="IV353_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9010259.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            complement(486..1040)
                     /locus_tag="IV353_002023"
                     /pseudo
     CDS             complement(486..1040)
                     /locus_tag="IV353_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(1056..1535)
                     /locus_tag="IV353_002024"
     CDS             complement(1056..1535)
                     /locus_tag="IV353_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9010260.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1541..2565)
                     /locus_tag="IV353_002025"
                     /pseudo
     CDS             complement(1541..2565)
                     /locus_tag="IV353_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2582..3688)
                     /locus_tag="IV353_002026"
     CDS             complement(2582..3688)
                     /locus_tag="IV353_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9010261.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3917..6514)
                     /gene="adhE"
                     /locus_tag="IV353_002027"
                     /gene_synonym="adhC"
     CDS             complement(3917..6514)
                     /gene="adhE"
                     /locus_tag="IV353_002027"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9010262.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6819..7199)
                     /gene="yajC"
                     /locus_tag="IV353_002028"
     CDS             complement(6819..7199)
                     /gene="yajC"
                     /locus_tag="IV353_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9010263.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(7296..8441)
                     /gene="tgt"
                     /locus_tag="IV353_002029"
     CDS             complement(7296..8441)
                     /gene="tgt"
                     /locus_tag="IV353_002029"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9010264.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8659..9357
                     /locus_tag="IV353_002030"
     CDS             8659..9357
                     /locus_tag="IV353_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9010265.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9525..12164)
                     /locus_tag="IV353_002031"
     CDS             complement(9525..12164)
                     /locus_tag="IV353_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9010266.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12783..13814)
                     /gene="queA"
                     /locus_tag="IV353_002032"
     CDS             complement(12783..13814)
                     /gene="queA"
                     /locus_tag="IV353_002032"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9010267.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            14007..>14602
                     /locus_tag="IV353_002033"
     CDS             14007..>14602
                     /locus_tag="IV353_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9010268.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         4096 a         3192 c         2608 g         4706 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcaaaagcgc ctacgagata acctcccata aacaaagcag agatcgaagc accaccagca
      541 acaagtgaat acatgataaa agcagtactt ggaggaatga ccatacctgt cggagctgac
      601 gcgatattga ccgctgtcgc aaacttccgg tcatatcctt cttccacttg ctgagggatc
      661 aacactccac caatcgcggt agaagcggca atggccgagc tagagatgga accaaataaa
      721 gcattcccta acacattcgt atgagccaaa gcccccggca cgcgcccgac gaacaacatc
      781 gcaaaatcca ctagtttctt agctatccct ccattattca tgataatgcc cgagagaacg
      841 aaaaacggaa tagcgatcag cgaaaaacta ttgatacttc ccaccatttt ttgagctgaa
      901 ctaaaagcag aaacatcaaa cggtataact aacaataaag tcatcatcga tgagaatgca
      961 atacttatag caatcggcat ccctaataca agcaacacaa caaatacaaa aaataacact
     1021 aatccggctt ctattgccat tttattcctc catttttata ttttttcttg aattgctggc
     1081 ttgttgctta atgtatcgat tagattgatc aggctataga taatgatgag tacccccgat
     1141 acaggtactg acagataaac atatcccata ggaatactta aagaaggcga gatctgcccc
     1201 attgtcaatg tgacagcatt ccatccgcca tacaccatga tcatcgcagc aaataccaga
     1261 aataaacttt gaacaacaac ttctaaaatt ttactttttt tgccagttaa tttttcactt
     1321 aaaaccgtta cggctaaacg tgctttttgt cccacaacat acgcggaaga caacatggca
     1381 aaccagacaa gaccgaatcg aacaagctct tccgtgaccg tatttggatt tcctaaaacc
     1441 gttctggaaa atacttgcca taaaacaaca gcgaccatcc caacaagcaa aacagctccc
     1501 aggatctcta ataccttgtt taaaatcatt ttcattacag ctacgctcct tgctcttgga
     1561 tcttttgata aaggtctttg taggtaggat cgtttttgaa tatatcatga agtggggcaa
     1621 ctgcctctcg aaacggtgta ttatctacat cattgaattg gactccatgt tcttcttgtg
     1681 ctaaagcttt ttcttcttca attgcttttg caaaagcttc aatttcaaaa accgttgatt
     1741 ctttcgctgc ttcttcaatg gctttttcct gttcatcagt tagctgttct ttgatggcat
     1801 cattcatgat gatgatatct ggcacacgtg tatgctcatc ataggaatag tatttagcta
     1861 ccccgccatg tccagctgta tacaagacaa attcattgtt ttctgatccg ttgatcaaat
     1921 tggattgaag agacgtgtac acttcatcac ttcccatagg tacgggcgaa ccgcctaaaa
     1981 gctctaccat tttgatagcg gtttcgcttt gcatcacacg gatcttcttt cccttcaaat
     2041 cttccggcgt atggatcgga ccgtctacca tataaaagct gcgctgacca gaatcataat
     2101 aggtcaatcc ggtaaatcct aaatttttgg tcgattggta gattggctcc attatttctt
     2161 cattatccat caccttaaag aaatgttctt catcattgaa tagataagga acggaaaaaa
     2221 tcgaataatc tttagagaaa ctttccaacg ctgaggcact tacttttgtg aaatctactg
     2281 ctcctgactg tgtcaattcg atcagttcac gttctccacc cagttgtcca tctgggaaat
     2341 actggatctg tacggaacca tctgtttttt cttttactaa acgcccaaat tcattcattg
     2401 catcaattac tggctgttgc ttgcataagc aaagcgcaag gtaactatct catttacgtc
     2461 tgcatctgcg acagttgcca cactagaatt atttccgcag ccgcttagac ctagcagacc
     2521 taggctaagc gaacttgctg caaacagcag tgatgttttt ttcatatggg tttcctttct
     2581 tttaacaata agagacgagc aattctgtca aacaaagcac cgttaacgac tgtccataag
     2641 gcattgctgt gattccgata ttccgataaa aatctaaact gtcccccatt cctgtcccga
     2701 ttgaaacgtt ttgaacttct cctttttcat cgatttgttc aagcaatcct tggatagcac
     2761 gataagcgac ttctttatat tcttgtggta aataacgttt atggaccgct ttcaaaatac
     2821 cgtaagcaaa tcctgcagtt gcagaagatt ctacgtaaga agaaggatca tccaacaacg
     2881 tatgccataa accgctttca gactgataag tttttaatgc acgcacttgt gcttcaagtg
     2941 tttcgatcaa taatttgcgc aatgcatctt ctttcgataa ttctaagatt tcaatgatct
     3001 ctggaatagc aatcgtcaac cagcagtttc cacgagccca aaatgcttcg gcatagttat
     3061 gatttccttc aaacgtccag ccgtgatacc acaatcctgt tcgtttatcc atcaagtatt
     3121 tgatatggat cataaactgg taacgagctt cttctaaata ttctgggcga ttcaacagtt
     3181 tcccgatttt tgccaaagga agcactgtca tcatcaatgt atcgtcccac aactgattct
     3241 tattttccgg tccatatgtg gcatgctgta acccgtcttc atttgtccga ggcatgtcat
     3301 tcatgaccca ttctgcccat tgttctaaat aaggcaaata gcgtgaatct ttcgtgtctt
     3361 catacaagta agccatagtc aatagcggtg ccattgtatt gacgtttttg ggcggagcgc
     3421 cttcttccat acgatcctca aaccattcat tgaccacttg ataagcgcga ggattttttg
     3481 tcatctggta atttttataa ataccatata gaccgacacc ttgtggccaa ttccacactg
     3541 tccagctttt gtcatctact ttcagtccat caaagtccaa caaaaattca ccattatcgt
     3601 ctttgatcgt tgtcaggttt tcaacgagca ggtcaatttt ttctttcact aattctttag
     3661 gtacaaaaat ctcttcttta ataatcatct ttacgccacc tttttaaacg ctttcatttt
     3721 atatccttaa tatagcagta ataccgtcca ctataaatat cgctttttga acaaaaactg
     3781 tctaaaattg acctaaatgt taaataacaa aagtaacttt cagttacttg gaaattaaaa
     3841 aaagaacttg gagaaacaac gttttccaag ttctttttca tctattttct tatctttttt
     3901 ctacgctttc ttttgcttat ccgttgtaag catcaatgat gatctgtttc aattcagaga
     3961 tcaatggttc tttcggatta gctgtcgtac attggtcttc gtaagctaat tcagccatac
     4021 ggtctacggt tgtatctagt gtttcttgtg tgactccttg agcttttaga ctcatgttga
     4081 tgccgacaga ttttcctaat tcataaacag ccgttgctaa agcttcgact aattcctctg
     4141 ttgtttcgcc tttcagacct aagaattttg cgatatctgc gtaatctgta tctgcacgga
     4201 aatagtcata tttagggaac atcgcatgtt tttgtggatc ttttgcatta tagcggataa
     4261 tatgcggtaa aagaatcgca tttgtccgtc catgagggat cccgtattcc ccgccgattt
     4321 tatgtgcgac tgagtgacaa atacctagga atgcgttagc aaacgccata ccagccattg
     4381 tagaagcgtt gtgcattttt tcacgtgatt ccatatcagg tgtttttact gatttttcta
     4441 aatagtcaaa gactaacttg attgcttgta ggctcaatcc acgtgtataa tctgaagcca
     4501 tgactgaaac ataggattcg attgcatgtg ttaacacatc catcccggta tctgctgtga
     4561 cagatgctgg aactgacatg acgaattgag gatcgacgat tgcgacatct ggcgtcaatg
     4621 cataatctgc taacggatat ttcacatgtg tttcactgtc agtgatgacc gcaaatggcg
     4681 ttacttcaga accagtccct gatgttgtcg ggatacatac gaattgtgtt ttttctggtt
     4741 tgtcgatctt atatgtccgt ttacggatat ccaagaattt ttgttttgca ccaaagaaag
     4801 atgtgtctgg gtgttcatag aacatccaca ttcccttagc tgcgtccatt gcagaaccgc
     4861 ctccaagagc gattactgta tctggcttga agtctgccat cagtttcgtt cctgcgtata
     4921 cggtatttgt cgatgggttc ggttcaacat ctgagaagac ttcgatcttc acatcatttt
     4981 tacgtcgtgc caatacttca cgaactgtat cgcagtaacc gaattggacc ataccagggt
     5041 cacaaacgat catgacacgt tcaacgtttt ccattttctc taagtacaat agagagttct
     5101 tttcaaagaa gatttttggc ggtaatttga accattgcat gttatttctc cgtttcgcta
     5161 cagttttgac attgattaag tttacagcag agacgttacg agaaacagag ttcttaccat
     5221 aagaaccgca tcccaaagtc aatgacggaa tcatttcgtt atagatatca ccgatgccgc
     5281 cttctgcaga tggtgtattc actaaaatac gacaagcttt cattcgaaga ccgaatttga
     5341 catgcaactc ttcgttttct gaatggataa ctgctgtatg tcctaatcct ccaagattca
     5401 acataccttc acacaaatca aacgcatggt ccgtgttgtt agctttgatc atggcaagaa
     5461 ctggagaaag tttttctctt gatagcggat aatctgggcc tacgccttcc aattcagcaa
     5521 tcagcatttt cgttcctttt ggtacactga ttcctgccaa ttttgcgatt tccatcgcag
     5581 aatgtccaac gattgatgga ttgaccgcat attttccttc gttcatcact gcatcttcta
     5641 atttttgcaa ttcatctggt tttacgatat aaacttggtg tgcttggaat tctgctttta
     5701 ctgctgcata gatttcttta tccacgatca cagcttgttc agaagcacaa atcattccat
     5761 tatcaaatgt tttcgaaacg atcaaatcgt tcactgcacg tttaattttt gctgtttttt
     5821 caatataagc tggtacgtta cctggaccta cacctaaagc tggttttcct gtagagtaag
     5881 cagacttgac cattccagct ccacctgtcg ctaagacgat cgcgatacct ggatggttca
     5941 ttagcataga agtcgcttcg atcgaaggat gttcgatcca ttggatacaa ttttcaggag
     6001 ctccggcagc gatagcagca tcacgtacca ctcgtgctgc ttctgcagaa gatttttggg
     6061 cacttggatg gaaagcaaaa acgattggat ttcttgtttt caacgcgatc attgatttga
     6121 aaatcgttgt ggaagtcgga ttcgttgttg gagttactcc gcaaaccaca ccgactggtt
     6181 cagcaatttc aatcaaccct ttttggacat cttcattgat cacaccaact gttttatcat
     6241 gtttgatatt atgccaaata tattcggacg catacatatt tttgattgct ttatcttcgt
     6301 aaacaccgcg gcctgtttcc tctacagcca tcttcgctaa tggcatatgt tgatccaaag
     6361 ctgccattgc catttcatgc acgatatggt ctactttttc ttgatcaaag ctttccattt
     6421 ctttcaatgc tgcatttgct ttttctgcca aatcatcaat cattgcttgt acgtcaattg
     6481 tcttcgtttt ttctttttct attactttag ccatcattat cctcctaatg ggtcatggtt
     6541 atttgttaaa taattcacat gtatatcata caatatttat ttttctatgt aaacactttt
     6601 ttgtgatttt tttcacagtt atttttaaat ataaaattgc tttatttaca ggtgtttgta
     6661 tagaaaaata tataaagtta tttattatta tttatatgac acacccgatt attcaatatt
     6721 gagcaatata tcccatgctc ttatctcttt cttttttgaa taaaaaatga ggtaggacat
     6781 ctttctgccc tacctcgtct ttaaataagc tttgatactt atttttcttc cttctcttct
     6841 gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa ctgtctcagc tggttcttct
     6901 gttgtttcaa cagtagtcgt tgcatcattt gttacgacag tccctggttt gaccgttttg
     6961 attgctgcac gatcgtactc aaggaagatc ccttcgcagt ctaaaacaac tgtccgtttg
     7021 tcattgtcga tttctgaaac aactccatgt aatccgccga tcgttacgac tgaatcacct
     7081 ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt gtttcttttg cgaacgtgac
     7141 ataaaaaacc acattgctaa tagcgcgata aacatcacaa gcattggtaa tgttcccatt
     7201 tttttcacct cactaattct gtatctacaa aaaacacttt agcagaaaaa tgctgataac
     7261 actagttttt tacttaattt ttaaaagatt ttcctttaga agctttttgc gttttcttta
     7321 ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa gcaaattatc atccatgatt
     7381 gcttgacgta cttgtttcat tagattcaac aagaagtata aattgtggta agaagtcaaa
     7441 cgaatcccaa atgtttcatc acatttgatc aaatggcgga tataggcacg tgtatagttt
     7501 ttacatgtat aacaatcaca tttctcatca attggacgga aatcatgggc atatttcgca
     7561 tttttcacta ctagacggcc ttgagaagtc atacatgtgc catttcgagc gattcgagta
     7621 ggcaagacac agtcaaacat gtctactcca cggataacac catcgatcaa tgaatcagca
     7681 gcaccgacac ccatcaagta acgtggtttg ttttcgggga tcaacggtgt agtgaattct
     7741 aatacacgat tcatttctga tttaggttcc ccgacagata acccgccgat tgagtaacca
     7801 gggaaatcca tcccgactag atctttcgcg ctttgacgac gaagatcttc aaaccctgct
     7861 ccttggatga ttccaaataa tccttgacgg tctggattag catgagcttt caatcctcgt
     7921 tctgcccatc gagacgtacg ttctactgat tttttcacgt agtcgtaact ttcatcgaat
     7981 ggtggacatt cgtcaaaact catcatgata tcagagccca atttattttg gatgttgatt
     8041 gctttttctg gtgataaaaa catacgcgaa ccgttcaagt gatttttgaa atgcacgcct
     8101 tcttcttcga tattccgcat atcacttaat gaaaaaactt gaaagccgcc tgaatctgtt
     8161 aaaatcggtt gatcccagtt cataaattta tgcagtccgc cagcttcttc tactaagtct
     8221 tcaccagggc gtagccataa atgatatgta ttacttaaaa taacgccagc acccatttct
     8281 ttcaactctt ctggggacat tgtttttact gtagctaatg ttcctactgg catgaacatt
     8341 ggtgtgggga aagttccgtg tggcgtgatc aattccccta aacgcgcgcc agtatgtttt
     8401 tctttcttga ttaaacgata gcgaatggca ggttgcgtca ttcatttccc tactttctat
     8461 ataaaatatc ttgacaactc ccaccatcat aatcgtttca agtgcttttt gcaagttccc
     8521 tcggcttcct tctactttat ttaatattct tttttttatc ctttttttat gaataacaaa
     8581 aaaaagaaaa acattatgac agaaatattt tttgatacat ggtatattgg tgttattaaa
     8641 gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa aacgccaagc aaaagagagt
     8701 ctaaaaggac gttggggaca agctgtttta ctaaatctaa tccctacatt gattggtatt
     8761 gctactattc ttcttattat gattcctatt atcttgtttg ccaccaccat atatgatgga
     8821 tcagcgagcg tctcagaagc gacaacttca ggaggcggta gttcaggagg cggtggcgga
     8881 attatttcca gcatcattag cgctttgttc atgagcggta tttcttggac ttatcttgat
     8941 attttacgtg gacaaaaaga tcagatcgaa ccattcaaag atgcatttcg tggattcaaa
     9001 ggtttgttta tcggcggagt gatcagctta gctgtgctga tctcgatctt tacaacatta
     9061 tggggtttgc tattagttat tcctggaatc attaaaagct attcttattc tcaaagctat
     9121 tttatttact acgatatcgt ccaagaaaca ggacaaacac ctaaagtact ggatacgatc
     9181 actgcaagtc gaaaattgat ggatggttat aaaggacaac ttttctggtt agatctttca
     9241 tttatcggct ggcatatctt agcattagca actgctggta ttggttatct atggctcagc
     9301 ccatacattt ctgcaacaaa agcagcattt tatgaacaat tgccaaaaga tatttaaaag
     9361 agagtgtgac ttgtgaccga gggggtggca ggagaacacc gtttatcgag aggcagtaaa
     9421 agaaacattc agcccattag caaaaaggag gtgtgacaac attcatgtca cacctccttt
     9481 ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag ttaatcattc ctcttcttct
     9541 gcttccggta atcgatggaa cttgatcagt tcattcaccg cgattggtac gatagataaa
     9601 atccctacca agaaccagtg attcaaactg atgtgtacta ggccgaatag ttcttgcgta
     9661 aatggcagca aagcaacagc cgttgtaata gctaatgcca gtaacgccat ttctaccaaa
     9721 gctttattgg tcgctaaatt gactttgaag atcgattggc tgctgcgtac attgaacaca
     9781 tgcagaatcg atgaataagc taagatcaag aatgcaacag tttgaccgac atgctgactt
     9841 gcgtctaccc atgggctgat agtatcgaca taactaccta aataataacc aaacaaggtt
     9901 acgatggtga agacccctgc atttatggca attttttgca ataaacctcg agcaaagatc
     9961 ccttcatttt tcggaatggg tggctgatcc atgatgccgt gttccgctgg ctctcttcct
    10021 aaagcaaatc ctggaagtcc atcagcaact acattgacaa acagtagttg cacagctgta
    10081 aatggtgctc cccatcctaa cagcatcgca atcaaaacaa taaagatctc cgaaatatta
    10141 cagcttagca aaaagttgat agctttacga atattctgat aaaccgaacg tccttgactg
    10201 acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa tcatatctgc ggcactttgc
    10261 gccacatctg ttcctgtgat ccccatggca cacccaacgt tacttgcttt caacgctggt
    10321 gcatcattca caccatcccc agtcatcgct acgactgcac ccgtacgttg ccaagatttg
    10381 acgatccgga tcttgtcttc tggcgttacc cgagcataga cagagagagc tttgacacgc
    10441 gcatctaatt cttcgtctga taattgatgc agttctgatc ctgaaagtgc ttctgacgga
    10501 tcattcaaga tacctaattc tttcgctatg gcactggcag tcaccacatg atctccagtg
    10561 atcatgactg ttttgatgcc agcctttttc gctcgcttga ttgctccttt actttctgga
    10621 cgtggtggat cgatcattcc gatcagacca atcaaacgta agtctttttc cagtgcttca
    10681 gaagaaatat cttgcggttc ttcgtcataa gtagcataac caacagcaat cactctcagt
    10741 gcccgtttac caaagcgatc attaacgatc gccgcttgtt ctacatcgcc tgaactaaag
    10801 cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga tgtatttttc gcccatttgg
    10861 tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa gctcgtttac tcgtggatat
    10921 ttttcttcta attctgcctt tgtatgataa ttctcttcaa cagcccttac gatggctact
    10981 tctgttggat tacctttgat aacaaggtcg tcatcttctt gctcgaccgt cacatctgta
    11041 catagtgcag ccattttcaa tatttccatc gcgctgttcg tcattgaatc ttcgatattc
    11101 gttacttcat cttctttgct ccatacccgg cggacgcgca tttcattttg tgtcagcgtt
    11161 cctgttttgt ctgagcagat aacattagcg gttcctagcg tttcaactgc tggcaactgt
    11221 cggatgatcg catgtttcct cgccatcttc tgcacaccgt atgcaagtgt caacgtaaca
    11281 ataacagtca aggtttccgg tacagctgca acagccaagg aaatcgaagt catgaacatc
    11341 tcaaggagtg gttctccttg cagttcacca atcacaaata ctaaagccgc agccgctaaa
    11401 gcgatcatac tgattcgttt gcctagctga ttcaatcgga cctgtaaagg tgttttttgc
    11461 atagtatttt cgttcaaaag atccgcgatc ttacccattt ctgtagccat gccaatggca
    11521 gtgacgatcg ctttcccacg acctgcagca accgtacatc ctttaaatac catattaagt
    11581 tggtcaccta atgactcatc ttcttcagca ataaatgctg catctttttc aactgcttca
    11641 ctttctccag tcaacgccga ttcttctaca cgtaattgtg atgcttcgat caaacgggca
    11701 tcggcagaaa tagcatcacc ggactctaaa acaagaatat cacccactac cagttgctct
    11761 gactcaacag tggtcacttt gccgtctcgt atgacagttg ccgtttgttt attcatatct
    11821 tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga caatcgccaa taccgagttg
    11881 atcacaacga tagcgatgat cagcagtcct tcaaataaat ccccatgttc tgtagcaaat
    11941 gcggtataaa aggaaatcac cgctgctaca agaaggatga tcgttgtaaa atcggacaag
    12001 cttcgaaaga attttttgat catcgattct ttcggcgcct catcgaattt attcaatccc
    12061 tgcttttttc gccgttcatc cacttcttgt gaagacaacc cagtatctgg atctgcttct
    12121 gttaccttct ttacttcttc tattgtctgc ttgtattctt ccatcctatt cctcctttaa
    12181 aatcttcgta caaaaaaagg tacttatttt actataacat ccttttttta tttacttcaa
    12241 ataaagggct tatcgattat ttgttaagaa agcgtaaaat ttaaagtgct tatatttgca
    12301 agcgctacca aaggatgcta ttatatagat gtaaggaagg aggataccat caaatagaca
    12361 gaagaatttc tcgatagaac agtgtaagca aacaaagttg ttagaagtac aggtttcacc
    12421 cagacatgcg tgtagagtac aaacctataa atagtgaatg cgtatggatg tttggatcac
    12481 gaagtatccg aaacaagaag taatgcgata cgtaacaatt gaataacgta aacgtctgaa
    12541 aattgaatag gaacaagaga tgtttcaaat gttgaagatg taacgaaaac aaaatttttg
    12601 tttaacatga agaaattcaa caccgtgctt tgcatgtatg atggaaagtt acaatcattt
    12661 ctgtttattt gaatccttcc gaggtacaag aaagccgagc ttatcgagag ctcggctttt
    12721 tctttgtcat aaaatctttg taaagctttt ttgttattca aaactagcgg cttttcataa
    12781 ccttatttca cgaacatcgc atctccaaaa ctaaagaaac gatagcgctc ttcaatcgca
    12841 tgctggtaag cagataaagt caattctctt cctgcaaagg cactaactaa catgactaaa
    12901 gtcgattttg gcaaatggaa attagttgag aaagcttcta ccactttgaa ttcatagcca
    12961 ggcgtaatga aaatatcagt ccatccgctg tctgctttga tttctccatt aaatttcgtt
    13021 ccaatcgttt ctaatgttcg aattgaagtt gttccgacag ccacgatttt cccgccattt
    13081 ttacgtactt ggttcaatcg ctcagccgct tcttccgtca aacggtagaa ttcactatgc
    13141 atatggtgtt cttcaatttt atctacgctg acaggtcgga atgtacctaa ccctacatgt
    13201 agtgtcagat agactaattc aacacccttt gcttcgatct catcaagtaa ttctttcgta
    13261 aaatgcaatc cagctgttgg cgctgcagca gaaccatttt ctttcgcata gacagtttga
    13321 tagcgatctg ggtcttccag acgttctttg atatatggag gcaatggcat ttctcccaat
    13381 gattcaagaa tctctagaaa aattccttca tactcaaagt caatgatccg tccgccgtgt
    13441 tccaattctt ctttcactac tgcttgcaat ctgccatcac caaaagagat tcttgtccca
    13501 actttggcac gtttcgcagg tttgatcaat gtctcccacg tatctccttg tgtattcgtt
    13561 agaagaagga cttctaaatg cgcaccagta tctggttttt ctccatataa acgagcaggc
    13621 agaactcgtg tattattcat aactagcgca tcaccaggct gtaactcttc aatgatttca
    13681 tggaaatgtt tatcttccat ctctccagtt ttccgatcca aaacaagtaa acgagaatga
    13741 tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg gcaagtcaaa atcaaaatct
    13801 tctgttgtta acatatcgtc actcactttc tatcatgtgc ttcatcattc tatcatgttt
    13861 ttcgttctgt tggcaaaatc agcacttttt cacttctatt ttttgttttc ctaaataaaa
    13921 aaactaccct atttgtattt tttcattgtt ttaggcatac tagtaataga tagttcttca
    13981 ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca aaatgtgtca aaaatttata
    14041 aaggtggaaa agtcgctgta gaggacgtca atctttcatt tgaaaagggt gagttcattt
    14101 gttttatcgg tacgagcgga agcggaaaaa cgacgacgat gcggatgatc aaccgaatga
    14161 ctgatccaac acaagggaaa attttgattg atggaaaatc catccaagaa attgatccag
    14221 tggaattgcg acgtcagatc ggctatgtta tccaaagtat tggattgatg cctcacatga
    14281 cgatccgtga aaatatcact cttgtccaaa aattgttgaa agtcagtcaa gaagaacgga
    14341 ataaaacagc tgaaaaaatg attgatcttg tggaattgcc ccgtgagatg ttagatcgct
    14401 acccacatga attatctggt ggtcagcagc agcgtattgg agtcgttcgt gcccttgcag
    14461 cgaatcaaga tatcatcttg atggacgagc cattcggtgc gcttgatcca atcactcgtg
    14521 attccttgca agaccttgtc aaagatttac aagagcgttt gggaaaaacg atcgtatttg
    14581 taacccatga catggatgaa gc
//
LOCUS       DACDLI010000063        14107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDLI010000063 DACDLI010000000
VERSION     DACDLI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.063"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1254
                     /locus_tag="IV353_002034"
     rRNA            <1..1254
                     /locus_tag="IV353_002034"
                     /product="23S ribosomal RNA"
     gene            1348..1463
                     /gene="rrf"
                     /locus_tag="IV353_002035"
     rRNA            1348..1463
                     /gene="rrf"
                     /locus_tag="IV353_002035"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1477..1549
                     /locus_tag="IV353_002036"
     tRNA            1477..1549
                     /locus_tag="IV353_002036"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1510..1512,aa:Val,seq:tac)
     gene            1553..1625
                     /locus_tag="IV353_002037"
     tRNA            1553..1625
                     /locus_tag="IV353_002037"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1586..1588,aa:Lys,seq:ttt)
     gene            1633..1714
                     /locus_tag="IV353_002038"
     tRNA            1633..1714
                     /locus_tag="IV353_002038"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1667..1669,aa:Leu,seq:tag)
     gene            1732..1804
                     /locus_tag="IV353_002039"
     tRNA            1732..1804
                     /locus_tag="IV353_002039"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1765..1767,aa:Thr,seq:tgt)
     gene            1816..1887
                     /locus_tag="IV353_002040"
     tRNA            1816..1887
                     /locus_tag="IV353_002040"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Gly,seq:gcc)
     gene            1904..1989
                     /locus_tag="IV353_002041"
     tRNA            1904..1989
                     /locus_tag="IV353_002041"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1938..1940,aa:Leu,seq:taa)
     gene            2000..2073
                     /locus_tag="IV353_002042"
     tRNA            2000..2073
                     /locus_tag="IV353_002042"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2034..2036,aa:Arg,seq:acg)
     gene            2083..2156
                     /locus_tag="IV353_002043"
     tRNA            2083..2156
                     /locus_tag="IV353_002043"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2117..2119,aa:Pro,seq:tgg)
     gene            2176..2248
                     /locus_tag="IV353_002044"
     tRNA            2176..2248
                     /locus_tag="IV353_002044"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2209..2211,aa:Ala,seq:tgc)
     gene            2276..2349
                     /locus_tag="IV353_002045"
     tRNA            2276..2349
                     /locus_tag="IV353_002045"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2310..2312,aa:Met,seq:cat)
     gene            2363..2436
                     /locus_tag="IV353_002046"
     tRNA            2363..2436
                     /locus_tag="IV353_002046"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2397..2399,aa:Ile,seq:cat)
     gene            2460..2549
                     /locus_tag="IV353_002047"
     tRNA            2460..2549
                     /locus_tag="IV353_002047"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2496..2498,aa:Ser,seq:tga)
     gene            2562..2635
                     /locus_tag="IV353_002048"
     tRNA            2562..2635
                     /locus_tag="IV353_002048"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2596..2598,aa:Met,seq:cat)
     gene            2654..2729
                     /locus_tag="IV353_002049"
     tRNA            2654..2729
                     /locus_tag="IV353_002049"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2687..2689,aa:Asp,seq:gtc)
     gene            2731..2801
                     /locus_tag="IV353_002050"
     tRNA            2731..2801
                     /locus_tag="IV353_002050"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2763..2765,aa:Gly,seq:tcc)
     gene            2815..2888
                     /locus_tag="IV353_002051"
     tRNA            2815..2888
                     /locus_tag="IV353_002051"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2849..2851,aa:Ile,seq:gat)
     gene            2913..3000
                     /locus_tag="IV353_002052"
     tRNA            2913..3000
                     /locus_tag="IV353_002052"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2947..2949,aa:Ser,seq:gct)
     gene            3022..3093
                     /locus_tag="IV353_002053"
     tRNA            3022..3093
                     /locus_tag="IV353_002053"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3055..3057,aa:Glu,seq:ttc)
     gene            3116..3205
                     /locus_tag="IV353_002054"
     tRNA            3116..3205
                     /locus_tag="IV353_002054"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3152..3154,aa:Ser,seq:tga)
     gene            3218..3291
                     /locus_tag="IV353_002055"
     tRNA            3218..3291
                     /locus_tag="IV353_002055"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Met,seq:cat)
     gene            3310..3382
                     /locus_tag="IV353_002056"
     tRNA            3310..3382
                     /locus_tag="IV353_002056"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3343..3345,aa:Asp,seq:gtc)
     gene            3386..3458
                     /locus_tag="IV353_002057"
     tRNA            3386..3458
                     /locus_tag="IV353_002057"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3419..3421,aa:Phe,seq:gaa)
     gene            3463..3533
                     /locus_tag="IV353_002058"
     tRNA            3463..3533
                     /locus_tag="IV353_002058"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3495..3497,aa:Gly,seq:tcc)
     gene            3546..3619
                     /locus_tag="IV353_002059"
     tRNA            3546..3619
                     /locus_tag="IV353_002059"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3580..3582,aa:Ile,seq:gat)
     gene            3631..3702
                     /locus_tag="IV353_002060"
     tRNA            3631..3702
                     /locus_tag="IV353_002060"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3664..3666,aa:Glu,seq:ttc)
     gene            3968..5161
                     /locus_tag="IV353_002061"
     CDS             3968..5161
                     /locus_tag="IV353_002061"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9010269.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            5291..6763
                     /locus_tag="IV353_002062"
     CDS             5291..6763
                     /locus_tag="IV353_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9010270.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            6846..7634
                     /locus_tag="IV353_002063"
     CDS             6846..7634
                     /locus_tag="IV353_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9010271.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7701..8369)
                     /locus_tag="IV353_002064"
     CDS             complement(7701..8369)
                     /locus_tag="IV353_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9010272.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            8484..10196
                     /locus_tag="IV353_002065"
     CDS             8484..10196
                     /locus_tag="IV353_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9010273.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            10199..11971
                     /locus_tag="IV353_002066"
     CDS             10199..11971
                     /locus_tag="IV353_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9010274.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(12053..13174)
                     /locus_tag="IV353_002067"
     CDS             complement(12053..13174)
                     /locus_tag="IV353_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9010275.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(13246..>14107)
                     /locus_tag="IV353_002068"
     CDS             complement(13246..>14107)
                     /locus_tag="IV353_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP9010276.1"
                     /translation="EKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKIN
                     NLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDR
                     EKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQ
                     RELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFS
                     EDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQ
                     YRRSLELSKD"
BASE COUNT         4037 a         2546 c         3145 g         4379 t
ORIGIN      
        1 tagtcgagga gagaatccta aggtgagcga gagaactctc gttaaggaac tcggcaaaat
       61 gaccccgtaa cttcgggaga aggggtgctg atcatacgat cagccgcagt gaataggccc
      121 aagcgactgt ttatcaaaaa cacaggtctc tgcaaaatcg taagatgaag tataggggct
      181 gacgcctgcc cggtgctgga aggttaagag gagtgcttag cgcaagcgaa ggtacgaatt
      241 gaagccccag taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc
      301 gggtaagttc cgacccgcac gaaaggcgta acgatttggg cactgtctca acgagagact
      361 cggtgaaatt ttagtacctg tgaagatgca ggttacccgc gacaggacgg aaagacccca
      421 tggagcttta ctgtagtttg atattgagtg tctgtaccgc atgtacagga taggtaggag
      481 ccgtagaaat cggaacgcta gtttcgatgg aggcgctggt gggatactac ccctgcgtta
      541 tggccactct aacccgcacc actaatcgtg gtgggagaca gtgtcagatg ggcagtttga
      601 ctggggcggt cgcctcctaa aaggtaacgg aggcgcccaa aggttccctc agaatggttg
      661 gaaatcattc gaagagtgta aaggcagaag ggagcttgac tgcgagacca acaagtcgag
      721 cagggacgaa agtcgggctt agtgatccgg tggttccgca tggaagggcc atcgctcaac
      781 ggataaaagc taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg
      841 aggtttggca cctcgatgtc ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt
      901 gggctgttcg cccattaaag cggcacgcga gctgggttca gaacgtcgtg agacagttcg
      961 gtccctatcc gtcgcgggcg ttggaaattt gagaggagct gtccttagta cgagaggacc
     1021 gggatggact taccgctggt gtaccagttg ttctgccaag ggcattgctg ggtagctatg
     1081 tagggaaggg ataaacgctg aaagcatcta agtgtgaagc ccacctcaag atgagatttc
     1141 ccatttcttt aagaaagtaa gatccctgag agatgatcag gtagataggt caggagtgga
     1201 agtacagtga tgtatggagc ggactgatac taatcgatcg aggacttaac caataggtta
     1261 aaagaaaaga actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa
     1321 tttactcaat aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta
     1381 accatgccga acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt
     1441 gtgagagtag gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag
     1501 catctgcctt acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt
     1561 agctcagttg gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg
     1621 gctcatattt ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc
     1681 ttacggcgtg ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta
     1741 gctcagttgg tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc
     1801 ggcatcttta aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg
     1861 tcgcgggttc gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact
     1921 ggcagacgca caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg
     1981 tcctcggcat ttcaaatatg cgcccatagc tcaactggat agagtgtttg actacggatc
     2041 aaaaggttag gggttcgact cctcttgggc gcgttaatta aacgggaagt agctcagctt
     2101 ggtagagcac ttggtttggg accaaggggt cgcaggttcg aatcctgtct tcccgataat
     2161 tataaattta aacacggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag
     2221 gtcagcggtt cgatcccgct aggctccatt tcatttaaat aatgaatatc tttttcgcgg
     2281 tgtagctcag ctggctagag cgtccggttc atacccggga ggtcgggggt tcgatcccct
     2341 ccgccgcgat ttttaagagc ctggaccttt agctcagttg gttagagcag acggctcata
     2401 accgtccggt cgtaggttcg agtcctacaa ggtccattgt ggcgttgtaa gtttcatatg
     2461 gaggattacc caagtccggc tgaagggaac ggtcttgaaa accgtcaggc gggtaaaacc
     2521 gtgcaagggt tcgaatccct tatcctcctt tttcatatta tcgcgggatg gagcagtctg
     2581 gtagctcgtc gggctcataa cccgaaggtc gtaggttcaa atcctgctcc cgcaattaaa
     2641 aactaaaaac actggttccg tggtgtaggg gttaacatgc ctgcctgtca cgcaggagat
     2701 cgcgggttcg attcccgtcg gaaccgccat gcgggtgtag tttagtggta aaaccacagc
     2761 cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt ttatgggcct
     2821 atagctcagc tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt
     2881 taggcccatt agtacaaaat cttattggtt ttggggaagt actcaagtgg ctgaagaggc
     2941 gcccctgcta agggtgtagg tcgggaaacc ggcgcgaggg ttcaaatccc tccttctccg
     3001 tttcaatatt ttgttttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg
     3061 gcggtaacac gggttcgaat cccgtacggg tcattaattt aatatagttt tacatggagg
     3121 attacccaag tccggctgaa gggaacggtc ttgaaaaccg tcaggcgggt aaaaccgtgc
     3181 aagggttcga atcccttatc ctcctttttc atattatcgc gggatggagc agtctggtag
     3241 ctcgtcgggc tcataacccg aaggtcgtag gttcaaatcc tgctcccgca attaaaaact
     3301 aaaaacattg gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg
     3361 ggttcgattc ccgtcggaac cgtttggctc ggtagctcag ttggtagagc aatggattga
     3421 agctccatgt gtcggcagtt cgattctgtc ccgcgccatt atgcgggtgt agtttagtgg
     3481 taaaaccaca gccttccaag ctgttgtcgc gagttcgatt ctcgtcaccc gcttttagtt
     3541 tttatgggcc tatagctcag ctggttagag cgcacgcctg ataagcgtga ggtcgatggt
     3601 tcgagtccat ttaggcccat ttattttatt ggcccgttgg tcaagcggtt aagacaccgc
     3661 cctttcacgg cggtaacacg ggttcgaatc ccgtacgggt catggaagat actttttgag
     3721 tatctttttc tttttatctg aaagaatgat tttaaaaagc ttagcagagt tatctttatg
     3781 gatgattgtg ctaagctttt ttggacagtt ctaatgtaaa atatttttat attttttaag
     3841 caaaaaaatg aaaaaatacg agcagaaaac tgttttatct gctatacttg gttcgttgca
     3901 agttcccgaa aggatccgca ggacggagat gccttgtaac cgaatttaac tagggggaat
     3961 cattataatg gtagaaagac acttatttac atcagaatct gtttctgaag gacatccaga
     4021 taaaattgct gaccaaatca gtgatgcaat cttggatgca attttaaaac aagatccaac
     4081 agcacgagta gcgtgtgaaa cgtctgtaac aactggtctt gttttagtat ttggagaaat
     4141 atctacgacg gcatatgtag atattcaaaa agtcgtacgg gaaacaataa aagaaattgg
     4201 ttatacacgt gcaaaattcg gatttgatgg agatacagca gctgtattgg ttgcgattga
     4261 tgaacaatct cctgatattg ctcaaggagt tgatgaagca cttgagatcc gggatgaaga
     4321 taaaaaagac gtattagatg agattggtgc tggtgaccaa ggtttaatgt ttgggtttgc
     4381 tgtagatgaa acaccagaat tgatgccttt accaatcgct ttgagtcatc gtctggtacg
     4441 acgcttggca gatttgcgta aatcaaatga attaacgtat ttacgtccag atgcaaaatc
     4501 tcaagtaacg gttgaatatg atgatcaagg acaaccggaa cgcgtagata caatcgttat
     4561 ttcaacacag catgatgatg cagtagataa tgaaacaatt cgtcatgatg tcatcgaaaa
     4621 agtagtgaag gaagttattc cagctgaatt attagatgat caaacgaaat attatatcaa
     4681 tccgactggc cgatttgtca ttggtggtcc tcaaggggat gccggattaa caggaagaaa
     4741 aatcattgtt gatacgtatg gcggttatgc tcgtcatggt ggcggtgctt tttctggtaa
     4801 agatgcgaca aaagttgacc gttctgccag ctatgctgca cgttatattg ctaaaaacat
     4861 tgttgcagca gggcttgctc gcaaagcaga agtacagcta gcttatgcga ttggtgttgc
     4921 tcaacctgtt tcgatctcaa tcaatacttt cggtactgga acggttccgg aagaagaact
     4981 gattgctgca gtgagagaaa acttcgatct tcggccagca ggaatcatcg agatgcttga
     5041 tttgcgtcgt ccgatttaca aacaaacagc tgcttacggt cattttggtc gtacagatgt
     5101 agatttgcct tgggaacata cggacaaagt agatgcgttg aaagcaagtt tagcaaaata
     5161 agttatattc agctggaacg atcgccaagt ggtggttgtt ccagcttttt ttatggcagt
     5221 taacctcttg aaaaagatag taattattta tctattttta gatcctgtca taatgaaagg
     5281 gaggaataag atgaaaaaag aaacaaatgt tactttggtc acagtgagta ttttcattgc
     5341 aacgttcatg acggctattg aaggtacaat cgtaacaacc gctatgccaa cgattgtcgg
     5401 ttcattacat ggaatggaaa tcatgaactg ggtattttcg atttatttgc tgacaaacgc
     5461 tatgcttact ccgatttacg gaaaacttgc ggataaagtg ggacggaaac caatctttat
     5521 ttttgggaca ttattgttta ttatcggttc gttattttgc ggtttatctc aaagcatgat
     5581 tgggttgatt gctgctcgtg cgattcaagg gatgggtgct ggggcaatga tgccagtagc
     5641 tttgacgatc atcggcgatc tttattctat tgaaaagcga gcaaaagttt taggtttgaa
     5701 tagttcagcc tgggggattg ccagtgtttt tggaccgctt gcgggaggat tcatcgttga
     5761 aacgatcagc tggcactgga tcttttttat caatgtgccg atcggaattc tattaattat
     5821 tttgatttgg ctatatttaa atgaaccaaa aatcatacat gagtctaaac caatggatat
     5881 ttttggtagt tttagtttga tggccatgct tgtctcattg ctgttagggt tccaattatt
     5941 aggagacgaa ggaatgagtc cgcttgtttc tgctttgctg ctaggtagta tcgtaatgtt
     6001 aggtattttt gtttttgccg aaaaaagagc taaagatcct gtcatcatga tgcatttatt
     6061 caaaaatcca ctatttgttg tggtcaactt ggctgcagca ttggctagtg gttttttgat
     6121 gggaatcgat gtttatatcc ctatgtggat gcaaggtgta ttagggctga atgcagcaat
     6181 tggtgggctg gtattagcac cattatcact agtctggatg gtcggttctt tccttgcaag
     6241 caagtggatg actcaaatga aaactcattg ggtcttgaga attggactac taatcgcagc
     6301 tattgggggc gcttggttgt atcttatgcc tttcagaagt aactatttat ggttttttgc
     6361 tatatcaacg gtcatcggta taggcttagg tgtgaccatg actacctcga ctgtcagtgc
     6421 acaaaatagt gtatctcgtg accagcttgg tgtagcaact tcttttaata cgttagttcg
     6481 aacaattggt caaacagtca tggtttctat ttttggttta ttgataaatg gggtaactaa
     6541 acgagaactt gcagcagcaa atttaacaaa tgatgcagat atcatgaata aactggtgaa
     6601 tccacaaacg gctaaatata ttgatgcgga tattttgtta ccattaagaa aaattcttta
     6661 tgatgggtta cattccgttt atctagttgg cttgttactc gtgatcattg ctttggtctt
     6721 aacgcttttc gttcatgaaa aaagtggtaa aataaaacag tagtttcggt taagatatgt
     6781 acgtagccga atcattcaaa tttaaagtta ttcaaaaata acttcttact aaggaaggcg
     6841 aaaatatggc atttattcaa gcaaatattt attcgaatgt gttagaaatg gaagtcaatc
     6901 taaatgtgat actaccgcaa acaacaaaaa aggtgattgg caccagttca gagcaagaga
     6961 caacggatat acctgtttta tatttacttc atggaatggg tggcaatcat agtgtatggg
     7021 aaagacgtac gtcgattgaa cgttatgtag caaactacgg attagctgtc atcatgccat
     7081 ctactgatct tggctggtac acagatacag tttatgacat gaagtattgg acattcattg
     7141 cagaagagct acctgagatt tgtcatgaat tattcccgca attaacaaga aaaagagaaa
     7201 aaacatatgc cgctggatta tcgatgggcg gatatggcgc gttaaaacta ggccttgcaa
     7261 aatcagaaaa tttcgcagca gtaatctctc tttcaggcgc tgtttctgta ggaaatcgaa
     7321 tggatgatct tttgatggtt cgtaaagcat ctttttggga agggatcttt ggaccattgg
     7381 atcaaatcga aggttcaaaa aatgatccgc aatacttatt gaaagaacta gtaaaaagcg
     7441 gaaaaccagt tccaagattt tatatggctt gtggggaatc tgattttctt tatcaggcaa
     7501 atcaggaaat ggtatctgct atgcaggaac aaggcttggc ggtcaccttt gaacaaggac
     7561 ctggcgatca taactggatc ttctgggacc aatggattca acgtgcattg gcgtggctaa
     7621 tggaagaaaa ataaaaaaag agtcttaaaa agatagctcc tatagagatc acaaacatag
     7681 tgatctctat aggagctttt ttactgctcg ttttctcttc ttttattttg ttggcataag
     7741 ccttcaatat aggtttgtgc actcgttaga aagagtgctt tttcattcaa ttctgcatgg
     7801 gactgcatac tttctttaaa tccctctaat tgattttcaa ctacataatt acgcataaat
     7861 agaactgttt ctttcaaaaa ttcatttccg tccataattt tttgtcgaag ctgctgttca
     7921 tgctgctgat ccattaactg tccaatcaat aaattgtgga gagaggcaat ggcctgatga
     7981 ctttgggtga ctgaaaatcc ttcacgaaca agtaattgga tcatttgctc caaatgttct
     8041 aatcgactcg gaaaagcggg cacagtattg atttctaatt ctgccccaca agggaaacgt
     8101 gtataaaggt catagtaatt ttccatatac tgcagcaact gttcattcca aggcaaagtt
     8161 ggatctggaa gcgctaattc ttcttccatt gtttcagcca ttttttgcaa gagctgttgt
     8221 ttgtttttga aataccaata taaagcaggt gcttgaacat gaattctgtt tgcgatgttt
     8281 cgcatggaaa gctgcgaaag agcttgtgtt tcagccagga tatcaaaggc ggcctgaatg
     8341 atgattgctt gagaaagctt tatttccatt ttatcaacct cgtgtattgc attatctttt
     8401 taaacatggt aaattgttag tcggtctaat tttacagtgt taaattagtc ctgtcaattt
     8461 aaacaaaggg gtgtaatcaa cgaatgaaat taatcttacg acatgcgaaa aaatataaag
     8521 tagctgtttt tatttccttg ctgtccgtag cagtcatggt tgcagcagca ttatggcaac
     8581 ctaagttact acagcaagta ttggaagcta ttattacaga agataatgat gaaatgaaaa
     8641 caattggtat ttacctgatc agtctggctt tgctaggatt agccgcaggt gtaacgaaca
     8701 cgattttctc tgcaaaagta gctcaaggcg taagtgcaga tatccgagaa gaagcatttc
     8761 gaaagatcca gacattttca tttggaaata tcgaacagtt ctctgctgga aatcttgttg
     8821 ttcgtctaac aaatgatatc acgcaaatcc aaaatttagt gatgatttct ttgcaatcac
     8881 tttttcgaat tccattctta tttattggtg ccttcatttt agctatgatc acgatgccgc
     8941 aattatggtg gatcatcgta gcgcttatcg ttacggtatt tctgattacg gcactttcat
     9001 ttacaaggat gggcaagcac ttcatgatca ttcaaaaact gattgatcgt gtgaatagca
     9061 ttgcaaaaga aaacttacta gggattcgtg tagtgaaatc ttttgtacaa gaagaaaacc
     9121 aattgaagtc attcagcaaa gtaagtgagg acttaacaaa acacaacatc atagtcggta
     9181 cattattttc tgtgatgatc ccgtccttca tgctggctgc caatttggct gtggttgggg
     9241 cgatcttttt tgtgagcgat cttgttaaag atgatcctac gttaattggt ggaattgctt
     9301 cattcatgaa ctacttgatg cagattatga tggcaatcat tattggcgga atgatgatga
     9361 tgatgacttc aagagcagcc gtatcgctga agcggattgc ggaaatttta gatgctgaac
     9421 cagatcttac ttatttagac gttccagaac aagagttgac gggttctgtc acttttgacc
     9481 atgtttcttt tcgttatcct ggtgaggata cagatacgct taaagatatc agcttttcta
     9541 tcaaacctgg agaaatggtt ggtatcgtcg gggcgactgg tgccgggaag tcaacactag
     9601 cacaattgat ccctcgtcta tttgatccga cagaaggcaa agttgaagta ggtggtgtag
     9661 atcttcgtca agtaaatgaa aagagtttaa gacaaacggt ttcctttgtt ttgcaaaaag
     9721 caattttatt ctcaggaacg attgctcaaa acttgcgcca agggaaaaag aatgcaacag
     9781 aaaaagagat ggaacatgct tcttctattg ctcaagcaaa agaattcatc gaaaagttat
     9841 ctgatcgtta cgaagcacca atagaagaac gtagcagtaa cttttctggt ggtcaaaagc
     9901 aacgtttatc cattactcgt ggcgttatcg gtaatccaaa gatcttgatc ctagatgaca
     9961 gtacgagtgc actggatgct cgctctgaaa agctggtccg agaagcactc gatcgagatt
    10021 tgaagggaac aacgacgatt gtcatcgcac aaaaaattgc ttctgttgtc aaagcagacc
    10081 gcatcttagt attggatgaa ggcagattag tcggcgaagg aacccatgag gaattggtag
    10141 ctggaaaccg tgtctatcag gaaatctttg aaactcaaaa aggaacggag gagtaagtat
    10201 gactgatcta ataaaagcaa gtaaattttt ctatcactat ttgaagcggt acaaactctc
    10261 ctttttcttt atctttatca cagtgatcat tgctacctat ctacaagtaa aggcacctca
    10321 atacatcggg gaagcttttc aagaattagc taattacgta ggaggcttga tgcaaggcgt
    10381 agacgataaa agcaaatttg tttctgtaat atggaaactc ttgctgtttt atgtactagc
    10441 tagtgcagcc aactttatct atagtatttt gttcacacaa gtagtaggga agtcaacgaa
    10501 ccgaatgcgt atcggtctat tcaacaaatt ggaaaaattg acgatccgtt tctttgattc
    10561 tcatcaagac ggtgaaattt taagtcgttt taccagtgac ttagataata ttcaaaatag
    10621 tttgaaccaa gctttgcttc aagtcatcac gaatgctgtt ttacttgtcg ggatcttgat
    10681 catgatgttc cgccaaaatg tagagttagc ttgggctaca attgcttcca ctccagtagc
    10741 cgttttgata gctgtcttga ttatccgcaa agcacggaaa tatgtggatc ttcagcaaga
    10801 tgaagtgggg aaactaaatg gttatatgga tgaaaaaatc agcggacaac gtgtcatcat
    10861 cacaaatggc ttacaagaag aaacgattga aggattctta gagcataatc aaagtgttcg
    10921 cgaagcaacc ttcaaaggac aagcttattc tggtctacta tttccgatga tgcaaggaat
    10981 gtcactggtc aatacagcga tagttatctt tttcggcggc tggttagcat taaatggtga
    11041 tttggaacgt tcggtggcat taggcttagt tgtgacattc gtccagtatt cacaacaata
    11101 ttaccaacca ctgatgcaga tttcatctgg atacagcatg atccaattag cgattacagg
    11161 ggcaagacga ttgaatgaaa tatttgatga aaaagatgaa gtcaaaccag aaaacggtaa
    11221 agcatttgat ggtgttgaaa aaggtcttgc gttgcagcac gtagattttg gttattctcc
    11281 agaaaaacta gtcttgaaag atgtctcgat caatgtggac aaaggagaaa tggttgctct
    11341 tgttggtcca acaggatcgg ggaaaacgac gatcatgaat ctgctaaacc gtttttacga
    11401 tgtcaatgat ggttcagtag tcatcgatgg aatagatatc cgagattatg atcttgatgc
    11461 attacgtaga catgtaggaa tcgttttgca agaatctgta ttgttctccg gaacgatcaa
    11521 agacaatatc acattcggca aaccggacgc ttcagatgaa gaagtcatca atgcagcaaa
    11581 acaagcgaat attcatgact tcatcatgac gttagaaaaa ggttatgata cagagatatc
    11641 cgaagaaaat aacttattca gtacaggtca aaagcagttg atcagtattg ctcgaacgat
    11701 catcacaaat ccatctttat tgattttgga tgaagcaaca agtaatgtag acacggtaac
    11761 agaagcaagg atccaaaaag caatggatga agcaatcaaa ggaagaacca gctttgtgat
    11821 tgctcaccgg ctgaaaacaa tcttgaatgc agatagaatc gttgtcttgc gtgatgggga
    11881 agtcattgaa gaaggcagtc atcatgagtt attagcagaa aatggatttt atgcggaact
    11941 ttatcataat caatttgtat ttgaaagtta aattaaaaga acctcgttat aacggggttc
    12001 tttttatata ttttatgttg tttgttttta taaggggcag aaaaggggca atttaaattt
    12061 ctatttttcc tagtttttct actagttgat tttgcatatt tttagttgta tgagtataaa
    12121 tctttaatgt tgtttttgaa tcttcatgtc ctacacgatc cataatggct tttaatggga
    12181 ttcctaattc tgtaagtaga gaaatgtggg aatgccttaa catgtgagaa gaaacttttt
    12241 tatctaggtt taattctttg gatactgctc ttaaagattg attatatgct tgaatagata
    12301 tcgggttacc atttttagag atgaaaatgt atttatcttt attattctta attaatttgt
    12361 tttcttcaat taattgatct atgatttctt tagcattact tgataattca atggttctat
    12421 aagaacctgt atttttagtt gttgttttaa tttttaactc gtaatcataa gtaccagaaa
    12481 ccgtaatttt attaccatca taatcagatt cagtcaaaga tatcaattca ccataacgta
    12541 gtccagttaa aaacaaaaat ttagtaatta aagaataacg tgactgttga tgtgaacttt
    12601 tttgtttact agcaatcatt tgaacttctt cttttgataa ataaccatct ttttcttttt
    12661 ttatttcttc gtttgtctta ggttttcttg ctaaagaaac cccatttacg ggattatatt
    12721 ttaatccata tcgacggttg gcgaatttaa aaatcattcc tgtaaaagtt tttaaatgta
    12781 ttacataatt atatgataaa tttttttcaa aatagtagta atctagcata tcttgtataa
    12841 gattactatt aacgtttttt acaagtacat catcttttat atattttgaa attaatgata
    12901 gataaacctg agttgttcga taggaacttt cttttacagt tggcttgtat ttttcatccc
    12961 atttagtttt taattctcca aaagttagtg gtaattcttc atttcgtttt attttttctg
    13021 cgattttttc attcaaaagt aagttagctt gattccaagc ttggcgtgaa ttgctagata
    13081 aagtaacgga cacttttctc tttttttcag ttaagggatc cgtataacgt tcggtgtact
    13141 taaatttttt gtcatttatt ttttctaacc acattatttc tattcctttc gaatatacgt
    13201 tcctattaat tgttaaaaga aaaagcccga aggcttttta tatatttaat cttttgacaa
    13261 ttctaaagat cgtctatatt gttcagcttt agctaatttt gtaaatgtta ctgttccatt
    13321 ataattttct aaaacaactt tctctatttc gtctagtgaa actttgaaaa attcttttct
    13381 ttgattaatt ttattaactt cttgttttct aaaatgatta tgcaatttag tttccaaaga
    13441 cggagcgtcc tctgaaaaaa tcatcgcatg aacgtcaaat tcaaatggaa ccgatgcact
    13501 actcaattct tttattctat ccataggttc taatcttcta gtcataccaa ttttatatat
    13561 gtcttcacca aaagacccaa tattactaat tacatacaca taaccggctc tagtatttaa
    13621 ttctctttgt aatacatttt cacgtataac ttctaattct tttaattttt cttccagttc
    13681 ttgaatttta tttgcatata attctttttc cacatctgca ttagcttttt gcatatatgc
    13741 gattaatttg gaaatttcag aattgaattg acgttgttct ttatcaatac gtttcttttc
    13801 acgatcaatt tctctacgaa ctttttcctc ttctatcatc tgttctttta ttgctttttg
    13861 ttgttctttt tcttcggtaa ttttcactaa ataagagtaa tgaagttcta gttcttgaag
    13921 tttaaactct aaaagaccct tccttaattc agcattgtca ttcttgaata aattattaat
    13981 tttatcaaat atttttatga ttttttccct agtcgagtct atattagaaa tagttagttt
    14041 attgagtaat cctttcactt ctgaatcaaa tggaatgagc atttttttct ttaaattatt
    14101 tttctct
//
LOCUS       DACDLI010000064        14030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDLI010000064 DACDLI010000000
VERSION     DACDLI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.064"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            216..2381
                     /gene="acm"
                     /locus_tag="IV353_002069"
     CDS             216..2381
                     /gene="acm"
                     /locus_tag="IV353_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9010277.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2653..3138)
                     /locus_tag="IV353_002070"
     CDS             complement(2653..3138)
                     /locus_tag="IV353_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9010278.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3618..4259
                     /locus_tag="IV353_002071"
     CDS             3618..4259
                     /locus_tag="IV353_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9010279.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4278..4913
                     /locus_tag="IV353_002072"
     CDS             4278..4913
                     /locus_tag="IV353_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9010280.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4931..5746
                     /locus_tag="IV353_002073"
     CDS             4931..5746
                     /locus_tag="IV353_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9010281.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6065..7501
                     /locus_tag="IV353_002074"
     CDS             6065..7501
                     /locus_tag="IV353_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9010282.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7690..10137
                     /locus_tag="IV353_002075"
     CDS             7690..10137
                     /locus_tag="IV353_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9010283.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10367..11434
                     /gene="rlmN"
                     /locus_tag="IV353_002076"
     CDS             10367..11434
                     /gene="rlmN"
                     /locus_tag="IV353_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9010284.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11627..12730)
                     /locus_tag="IV353_002077"
     CDS             complement(11627..12730)
                     /locus_tag="IV353_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9010285.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13283..13831
                     /gene="aac(6')"
                     /locus_tag="IV353_002078"
     CDS             13283..13831
                     /gene="aac(6')"
                     /locus_tag="IV353_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9010286.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4814 a         2313 c         2966 g         3937 t
ORIGIN      
        1 aaataaaatg aatatatgcg aaaaaataat ttacattagg aaaaaccttg taaaaacagt
       61 tttttggcat atttattttt tataaatatt tgtaaaataa ctatcaattt tttagttaaa
      121 agattggaaa tgagtggggg aggaattgaa gaaaggattt ttgagagatg atatagtagg
      181 ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga aatattattt
      241 tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat gtctatgcgg
      301 atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca acgaatatta
      361 cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa aatatccaac
      421 caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt gaaggattcc
      481 aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg gtcagaaaag
      541 attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta gaaggttggg
      601 ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat acgggaccat
      661 tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt
      721 cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat actaagcaca
      781 ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca gttaaaatca
      841 gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt aatcaaatac
      901 acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt ttacaagatt
      961 tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata actataccaa
     1021 aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata gaaaacccag
     1081 aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat aaacctgctg
     1141 ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt ggagtcaacg
     1201 gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag attggtatcc
     1261 caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga cagtctagta
     1321 tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa gtaggagact
     1381 atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta agttcaaatg
     1441 aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct atttataaca
     1501 agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc actccaagac
     1561 cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg
     1621 cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat atccaacagt
     1681 atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa aacggaaatg
     1741 attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca aatgagaata
     1801 aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac caagacggca
     1861 aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc caacacaaag
     1921 aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa tacagagacg
     1981 ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt acgacgattg
     2041 acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct ataccaggta
     2101 gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa attagaaatc
     2161 tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa caaagaagca
     2221 attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa agcattcttc
     2281 ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg atagtaacta
     2341 tctgtgctgg gatattctta caaaaatata aaaaagttta gttagaatat aatatttttt
     2401 gttaaatatg actgaaatca aaagaataaa attccgattt ataatcgaaa aaagaagcat
     2461 ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta actatggaag
     2521 aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat tggccaaaag
     2581 aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg aaataagaaa
     2641 actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa caagtatctt
     2701 ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat gaaagcctac
     2761 ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct ctttgtcgtg
     2821 catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag gaagctgttc
     2881 ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt ttaaatcggc
     2941 gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg atttcaataa
     3001 tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg acgtaatcgc
     3061 aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat aagcttcttg
     3121 ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat cataaaaaag
     3181 agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga
     3241 aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa gaggcagcag
     3301 ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga aaatccgggg
     3361 agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt tccgtgagga
     3421 gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga tcaaaggact
     3481 ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa
     3541 cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat ttttttattt
     3601 taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta ttagtaggag
     3661 cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca ttagggattg
     3721 ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt caaacatatg
     3781 tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt tatgggttgc
     3841 cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt atcttaaact
     3901 atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct caaggtcaat
     3961 atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc
     4021 cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac ttggtcaaag
     4081 attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga
     4141 tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat cttacattga
     4201 ctggtattct aacaatcgta atgaaacaag ttgaaggaaa attaaattat taccgctaac
     4261 tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt ggtgaaaaaa
     4321 aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct atcgtaggtc
     4381 cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa agttctgaca
     4441 gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac
     4501 aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat atttttgaaa
     4561 acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac accaaaaaag
     4621 cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat ccttatcagc
     4681 tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg aatccacggg
     4741 tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag caagtcgagc
     4801 gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc catgatttag
     4861 tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat taagaaagga
     4921 gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc ttcttctttt
     4981 tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca acgaagagaa
     5041 aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag ataaagacgg
     5101 gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat atgggattac
     5161 ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca atggaacgat
     5221 cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag tgcaatttac
     5281 aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag gaatcacaaa
     5341 ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg gttatgatgt
     5401 atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg ctgttttgta
     5461 tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat
     5521 ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag acttcaacat
     5581 aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt cagatagtac
     5641 attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg aattccaaaa
     5701 aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa taggggactg
     5761 tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct gccaactcct
     5821 tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt caaaagccag
     5881 tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt
     5941 cttactactc gaaactaagc gactttttca caacctcctt tacatatttt tattttgttg
     6001 tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg aaaggaagag
     6061 atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg gctctgcttc
     6121 tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat cagtctggga
     6181 tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt tggcagtaga
     6241 ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc tgaaagctta
     6301 ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag tcaatcaagc
     6361 tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata ttgaacctat
     6421 gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata atggttggga
     6481 atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg aggcattccg
     6541 cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt cattaggtta
     6601 tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg aagttaacca
     6661 tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag aaatgcctgg
     6721 aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta atcctgaaaa
     6781 tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg atgtctatat
     6841 gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat gggcaccaac
     6901 gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc ttgggatcaa
     6961 ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg ctggaaaaat
     7021 gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa tgtataaaaa
     7081 agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc cagaaggatt
     7141 aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga ttacggaaaa
     7201 tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg actatcgaat
     7261 caattactta caaagccacg tcaaagccat caaagaagca atttcggatg gagcagaagt
     7321 cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg gttatcaaaa
     7381 gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt tggaacgata
     7441 taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag agaaccttta
     7501 gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt cttgataaaa
     7561 tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc
     7621 tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg atgaaatgag
     7681 ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg
     7741 aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt
     7801 ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat tgcaaacggc
     7861 tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc cgtattagga
     7921 ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg ctggtttggc
     7981 ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc
     8041 catactgggg aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa
     8101 caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt
     8161 acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga
     8221 ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt
     8281 ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg
     8341 gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca
     8401 aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa
     8461 agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt
     8521 tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta
     8581 ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat
     8641 gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta
     8701 ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa
     8761 attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag
     8821 gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg
     8881 gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa
     8941 gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat
     9001 ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat
     9061 aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt
     9121 tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt
     9181 aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg
     9241 atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg
     9301 gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa
     9361 gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt
     9421 gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg
     9481 atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt
     9541 attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt
     9601 ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt
     9661 gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc
     9721 ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa
     9781 ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa
     9841 gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca
     9901 gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg
     9961 gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta
    10021 gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa
    10081 gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac
    10141 ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca
    10201 aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt
    10261 ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat
    10321 catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc
    10381 catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa
    10441 attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga
    10501 aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc
    10561 tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact
    10621 ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg
    10681 tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa
    10741 aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta
    10801 ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga
    10861 gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg
    10921 attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa
    10981 ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa
    11041 tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga
    11101 agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt
    11161 gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa
    11221 gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata
    11281 ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt
    11341 aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg
    11401 aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta
    11461 ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt
    11521 ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg
    11581 tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct
    11641 tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt
    11701 taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt
    11761 tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg
    11821 gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca
    11881 tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt
    11941 tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt
    12001 taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg
    12061 catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac
    12121 gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc
    12181 cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata
    12241 agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt
    12301 ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag
    12361 tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt
    12421 caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg
    12481 ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc
    12541 cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt
    12601 ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga
    12661 cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac
    12721 tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga
    12781 tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa
    12841 taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa
    12901 aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt
    12961 gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt
    13021 atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc atgatgaaca
    13081 aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg aataaagtgt
    13141 ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag agatactgat
    13201 tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa
    13261 gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt
    13321 gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc
    13381 ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca
    13441 agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt
    13501 gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa
    13561 ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga
    13621 tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt
    13681 ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa
    13741 aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa
    13801 aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag
    13861 cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg aatttttgtg aaattccaac
    13921 ttatttccga agaagctact tttggaacac cgtgtaccag aggttgtgaa aaagtcgctt
    13981 agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg
//
LOCUS       DACDLI010000065        13624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDLI010000065 DACDLI010000000
VERSION     DACDLI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.065"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..1345)
                     /locus_tag="IV353_002079"
                     /pseudo
     CDS             complement(53..1345)
                     /locus_tag="IV353_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1479..2669)
                     /locus_tag="IV353_002080"
     CDS             complement(1479..2669)
                     /locus_tag="IV353_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9010287.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(3541..5172)
                     /locus_tag="IV353_002081"
     CDS             complement(3541..5172)
                     /locus_tag="IV353_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9010288.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(5186..6859)
                     /locus_tag="IV353_002082"
     CDS             complement(5186..6859)
                     /locus_tag="IV353_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9010289.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(6872..7189)
                     /locus_tag="IV353_002083"
     CDS             complement(6872..7189)
                     /locus_tag="IV353_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9010290.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(7214..8614)
                     /gene="lacG"
                     /locus_tag="IV353_002084"
     CDS             complement(7214..8614)
                     /gene="lacG"
                     /locus_tag="IV353_002084"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9010291.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            8851..9636
                     /locus_tag="IV353_002085"
     CDS             8851..9636
                     /locus_tag="IV353_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9010292.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            9639..10331
                     /locus_tag="IV353_002086"
     CDS             9639..10331
                     /locus_tag="IV353_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9010293.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(10605..10976)
                     /locus_tag="IV353_002087"
     CDS             complement(10605..10976)
                     /locus_tag="IV353_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9010294.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(11376..11606)
                     /locus_tag="IV353_002088"
     CDS             complement(11376..11606)
                     /locus_tag="IV353_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010295.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(11700..12038)
                     /locus_tag="IV353_002089"
     CDS             complement(11700..12038)
                     /locus_tag="IV353_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010296.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(12966..13082)
                     /locus_tag="IV353_002090"
     CDS             complement(12966..13082)
                     /locus_tag="IV353_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9010297.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         4209 a         2781 c         2258 g         4376 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gtctttttta
       61 acttttcttt agcaaatgag ttcagattat ttgctggatt cataacgata gatttgccat
      121 taaacgattt taatacattc atccgagata tttcagacac ttcagctaaa tgtacagaat
      181 gtgtggtcat gagaatctga gtatagtttt tttgaagatt taatccttcg aacagagttt
      241 tttgaatatg atgatgaata tgagcttctg gttcttcaat caacataata ttaagtagtt
      301 ctcttgaacg acacgcttca aattcgacta ttttcaaagc taaataaatc atatttagat
      361 gtcctaaccc tagtaagtct aagtcgatat tttgttctgg cttcattgaa agaaattttg
      421 ctaaagaacc catatcatct gacatagttg aggaaagaag aagattagga gaatacactg
      481 aaccaattat gctatttagt tgccgattaa tattttcgcc aattttccta atttcaggaa
      541 cactggtaat agtctcattt aattgctgta taattgtttt taatgactca acattatctg
      601 atgatatttt tgattcaatt gtttccatga ttcttttgac tgggtttcga ttgtttttca
      661 tttctctaag aacatctcga agagcgtcaa cataaacaac cgaaatatgc ttgaaaatat
      721 ctgacatgtc aattcttgag cccaacagag cttcatcgtc ttcttcagga ttactagcta
      781 tattgtcatt gagtcttcct accaatttat cataattttc attgatacag aaatttagat
      841 ttgatttaga tgtgtagtaa aattcatagt cggatagacg aatagaattt ctaaattcgt
      901 taaattgatg agagtctaat tcagtggaaa tatcaaataa ttgctttcta atagctttgt
      961 taggtctaat aaatagagaa atactaccta cacctttatc tgcatttgaa atcaattctt
     1021 ctaagattgt ttgactttca gaatttaaag aaatggaagc acaaatctca ttgtcaaatt
     1081 cttcttcgct gattccttca aatgtcgcag agataataat ccaatgccct tgccacccat
     1141 tttgaattcc atgaaaaaaa tctgattctt ttagtgtttt actactatag taataactat
     1201 catctaataa gattctcaat gcagtaagag cattggtttt ccctgaatca ttctcaccta
     1261 taatagtatt tgtctcatta tcgaatcgaa agatttcgga ttcataattt ctaaaatttc
     1321 gaatttgaag agtagatata aacataagca gatctcctaa ctatttaatt aatatgatta
     1381 tatagtaagt ttatcataaa ttatgttagt aaaagttctc attataaggg agcgtcaata
     1441 attttgtgta aatgattctt ccctactgca gattgtttct aattctcaat cagttggctt
     1501 agcatttgtt cgagctcgcc ttcggcctgt tggaaacctt tatgactgcg acctaggaat
     1561 ttttggttgt aagtatcaaa agacgagact aagaaacgtt ccatcgactc ttcattttgg
     1621 aactgttcct tgcggtggct gtatttcttg atttgtttat taaaggattc aatcaagttc
     1681 gtagaataaa tactcttgcg tatggccaag gggaaatcat agaaagtgag caagtgatcg
     1741 ttcgaaagaa tcgattcaac cacttttgga tagctggttt tccatttttc cgcaaaagta
     1801 ccacgtgctt ccaacgccac ctctttagat gaggcttgat acaccatttt aaaatcatca
     1861 cagacttcct tacgatcatc gacacgcact ttatgactga tattacggga aacgtgtaca
     1921 caacaatgtt gaaaacgagc tttgggatag aaccgactaa tcgctcctac catccccttt
     1981 aagccatccg tgatgaacag gaggacattt ttcaaaccgc gctcttgaag gtccaataaa
     2041 atttcctccc aaatcgtgat ggattcagtc ggtgcaatcg catagctcaa tacttccttt
     2101 gatccgtccg ggcgaatgcc aactgcgata tgaatagctt ccttggcgac ggttttccgc
     2161 tttaacggaa tatacgttgc gtccatataa atagcagcat aacggtcatg aagctcccgc
     2221 ccattaaacg ccgttacctc ttctgtaaat gattttgtta tattggacat ggtttggggc
     2281 gtgtagtaat gcccatacat tttctcaatc aagtctgcga tttccgacat ggtaatacct
     2341 tttcggaaga ggtgaatgac ggtctcctct aacgtgtcat tcgtccgtct ataagcagga
     2401 acagtctgtt gcttgaactc accattgcgg tcgcgcggaa tctggagatg aagttcccca
     2461 tactcggtct tgaccgtacg gtcataggag ccgttacgag aattacccgt gttaaaacca
     2521 atgcgatcat acttttcgta atctaggaaa gccgttaact ccgttttgag aagcgtattg
     2581 actgctttct ccaaatggac acggaataat tcattcaaat cgcctttgtt cgctagagtc
     2641 tttagaattt ctgtagtaaa atcgttcata gggaagtcct ctcttctgtg aatgtgttgt
     2701 ggtaactcta ttctacagaa gggacttcct tttttctatt tacacaaaat attttacact
     2761 ctccattata acaattgggt taaaaagaaa atagatagta ttctacctca tatttatttg
     2821 aaagtaatat ataaaagacg ttcaaattcg aacgcctctc gttaaccaaa tattggattc
     2881 agaatctagt tttcatatat aaacagagta tgattaggtg caaaaattgc acttttattg
     2941 cacttttatt tttatttata gtgttttact gttctgtata ttgttttaac tcccttgata
     3001 gtatcgcatt ctgttttata cactttacgt tgaaaacaga gtgggaataa agagccaaca
     3061 taatatggag ggggaatccc tataaatcaa cattcttcaa tagctaaaat gcttgatttt
     3121 cattaaaaat aagatggttc cctaccaaaa gagactataa tataagttaa tttgtttcat
     3181 tcttatcata aaaagaagtt ctcgaaaaac gaactgtttt ccgagaactt ttttctatcc
     3241 atctaagtgc tcaaatcata ttcacgacgg aattctaagg ttaccctttc ttaaacaaac
     3301 tttatgttat ctgcacaact ttttgcaatt ttttcattgc ttcttacgaa atcgttgaac
     3361 tctctttagc ataatacaaa gtgtagatga agtctggtgc tttactcgtt ttggtaaaaa
     3421 tgactggaaa tcgtttctgg ttatttttgg caaaggcgag gtgcaggttc gattcctgcc
     3481 ttctacgcta acgatgtggc tgaagggaac ctccgatctt gaatcgaaac gatccgcagt
     3541 ctattgtttc accaatgaaa caaaatcaac gttcgtccaa gcatttcctg aagccttgct
     3601 ataaacccca atttcacaaa cacctgttgt aattggaatg tctttgattg taatttttgt
     3661 ccattcactt gttttaacaa ccgcagtatt tagttccgct ccaccataat ttttgatata
     3721 taactgatta gtatcaaact ctcccccaga cattacatag gcactcaatg tgtaagttcc
     3781 tttgcttatg ccactaacat tttgataagc tgatgcctcg tatgcttgat cctgccaaaa
     3841 agttagtttg tagtccccaa aaaaagtgcc cgcttccgtt ttgatcgtac ccttagcggt
     3901 tccttgttga aaccaggttt gccaattgga aggactggtc gttggtccat cgctttcgaa
     3961 acttgcattt ttaactagat ttatttgcgc ccctgcttgt ttaaacgcac gcaagcttgg
     4021 caaggcgttt ccagcaaaat caaacatcgc ttggttgtcc cattcgtttc ccatagtgga
     4081 ttgaacactg agataattca tacctgcttg tgttgcccaa ctcacatttc cgttgtacca
     4141 aagaggttcc caatagaaga agcccgcccc gcgattattc ggtactgcct gaatccgatc
     4201 ggcaagatcc agcaaatact ctgcttgtcc tgtcggtgtt ggttcatatc cccccgcagc
     4261 tgcttcggct tcaccaaaag catttggcat agtatcggca ttcgctgttg tatgcccata
     4321 agctgtttca actacgatca cttctttacc ataccgttgg ctgatcgcat tcatattggt
     4381 ttgaagttca ttcattgaac cgtgccaata cggataatac gacactccaa ttaaatcaaa
     4441 tgacacacct cgactcgtga tttcatcaaa ccaccaagta aatgctcctg tatcgccacc
     4501 atcagctaag tggagcatta ctggagttgt tttaggttga gtgtctcgaa cagcttggat
     4561 accagacttc aaaaaagcag ccaatcgatc aaattctccc ccgccttctc cccaagactt
     4621 gccataaggc cagagcattc cgctattcag ttcattgccg atctgaacca tatcaggata
     4681 aacgtccagc tgcttcattt ccgacagaac gttctttgta tagtcgtaca atgccgtatt
     4741 catttcttca aaggtcagac cttgccatgc tttaggaaga ttctgtttcc caggatcaac
     4801 ccaaaaatca ctgtagtgaa aatccagcag caccttaagt cccagatttt ttgctcgttt
     4861 tgcaaggtca atcgtcgttt ccagatcatt tgtccctgct ccataacttt ctccattact
     4921 attataagga tcctgccaca aacgcagtcg aacatagttg acaccattat tttttaaaat
     4981 ctctaaggca tcttttgaaa tatcgtcttc ataatatttt gcaccagatt tttccatatc
     5041 tgacaggatc gaaatatctg ctccgcgaat aaattcgctg gcagatgctt caacagtttt
     5101 tgaaacagcc cccaaagccc ctaacacagc tgcaaataaa acagctttac gcaaccaaat
     5161 tcccattttc attatggctc ctcctttatt gaaattggtt cagaataaac tgaacagctt
     5221 tttcaggatc tctcgttaat ccgatatatt ctgctccttt tgtcgcaacc agcttgattc
     5281 ctaagcgatc ggtgtcttct ttgatatcat catagtaaga attgacttgc ggtgctaaga
     5341 ccaccatatc aaaatcctgc agcatttcgt aatgagaacc ataagcaccc gccattgcac
     5401 tgatcggtgc tttcgattca gatgcacctt tttttaaagc attagctaac atcgcacttg
     5461 tccctgcgcc agcacaaagt accaacacat tgatttcttt atttgcatcg ctgataactt
     5521 gactcgctgc cacaggtact tcctcacgta catcggcaga agcaccttcc tcggttgcga
     5581 tcacctctgc tttttgtgct tctttctcta ctaagacagc atcgtatgct ttgcaaaatg
     5641 gtagatagat caggaagtca acgacgatta gaaccgctgc tagaacaaaa gctaagatac
     5701 ccatgcccgt tcctagaacc aaaccgatcg gtgctggagt tgcccaaggc aaaacgtaca
     5761 taaagctgtt catgcccaat acatcgacaa aaaacttgaa caggcaaacg tttacaacag
     5821 gtgttaacaa aaatggaata aaaaaataag ggttcaaaat catgggtgcc gcaaatagca
     5881 atggttcgtt gacagcgaaa cttactggaa taaaggaagc tttcccaact gccttcaact
     5941 gcttagactt agcaaagagt aggaagataa aaggaacaac caatgttgcc ccagttcctc
     6001 ccatagttcc tacaaaattc cctaaaccca ccgtcagtac tttagaagct tgttcaccgg
     6061 aattgaacaa ttgcagattg gtttccacat tggcatagat aattgccgca attgccggct
     6121 ccacgatcga cggtccatga acaccgacaa accaaaacag cgccatcgct ccccagataa
     6181 tagcgattcc taagtacccg tcagctgctg taaatagcgg ctgcagcacc gtaatgattg
     6241 cttctgcaaa tgagatttgg aagatttgtc gaatgcctaa atcaattaaa acggccgcaa
     6301 atactgaaaa agagaaagga aaaatatctc ggaaggtttg cgaaatcgtt ccaggaactt
     6361 ctttaggcat tttaatggta atatctttca gcacacaaaa cttatataaa ttaactgtta
     6421 aaaatgcagc gacaaaggaa gccagtagtc cctttgtccc catatattcc gttgaaaatc
     6481 caccttcaat cggagagacg cttaacaaca ggaaacttac cacagaggcc agcatcacag
     6541 aggtggtgtt gattactttg tttttcggta agcgacgatt catcgaatca gccaagcagc
     6601 gagcagtggt tgctgaaacg agtaacccta caacacccat cgaataatta tagactttcc
     6661 atagccatgt ggagacagat tcaggccatt ggtaaccaaa aatctcagga accgcagcaa
     6721 tcaaaatgaa aatgcttgaa aataaaatag ctggcatcgc tgttaaaaat ccgtcacgaa
     6781 cggctcctaa ataactatta cgtgagattt tttcaaaaaa tggtttccct ttttcgattt
     6841 gtttaatgat tccatccata gcgttcttct cctatccttg attctgtctg tacagtacaa
     6901 taaagtcttg aatcagatct ctcagcaata aagtggtcat taaatgatcc tgcccatgga
     6961 taaaaatgaa tcccatctcc atctctttcc cagatgcttc ttccgctaaa atttttgttt
     7021 gcgaattatg tgcaagggtt aaattttcgt cggcttcttt caacgccgaa tcaacatgtt
     7081 caaagtttcc ttgtcttact tctttcaata atttcaataa tgtactgcga gcgtcgcctg
     7141 aatatgcaac gatctcaaaa ccaagcattt gtaattcctc tttattcatc ttttcttcct
     7201 ccattatcac ttattaaata atcgttttgg tttcactgac caacttgtac cagtaagctg
     7261 attcttttgg gtaacgcttc tgggtttcaa aatcaacata aaacaagcca taccttttgt
     7321 tatacccatt tgtccatgag aaaagatcca taagcgacca caaaaaatac cccttcacat
     7381 ttaccccggc tgttatcgcc ttgcttaagg attccaaata gactctcaaa tagtcgattc
     7441 tcggctgatc cataataatg ccatcttcaa actgatcttt ataccccatc ccgttctcag
     7501 tgatatagat tttgttgtaa tgtgggtaat cgcttttaat tcgcagcaac aagtcatata
     7561 gaccttctgg ataaatcagc cagtcccaat cagttcgagg aattccttct ttataaatac
     7621 gttctccgat tcccttcact ttataaacag aggtcccttt ttcacccgta ccgttatgat
     7681 ggattgcatt ttctccatcg taagctttga caaaatggca ttggtagtga ttgatcccta
     7741 agtagtcatt acgcgtggat gcttttttca attcaacgaa atcttcttca ggaaaatgat
     7801 agctggcctg atttgcttca catatctcat caagagcagt caaggtttca gttgaataat
     7861 agcctaaata ggtagcatct aataagaaac ggatcgacaa tgcatcgtct aaaaaggcag
     7921 catgcttatc ttccggcgcg tctgttgccg catatttcgt ctctaatgag tgaactacac
     7981 caatttcacc cggaagttcg ttttctttga aatagttcac tacacgggca tgagcaacca
     8041 tcatattatg aagacaggcg acgatctttg taaaatcata tttgattcct ggtgggaaaa
     8101 cacctaacaa atattgattg gttgcaactg gatagatttc attaaaggta ctccacacct
     8161 tgacttcttt gaattcatga aaacaaaaaa tggcatagga aacaaatgct tctattgttt
     8221 ctcgattcaa aaaatctcca tggtcaaaca aacgtttagg ggtatcaaaa tgatgcaacg
     8281 tcacaaacgg cgttacgttt cttttcttac attcttcgaa cacacgatga taaaaagcga
     8341 ttccttctgg atttggtttc ccagctccat caggaaaaat gcggctccaa gcaatggata
     8401 atcttagacc atttacacca aaacgttcac acaattcaat gtcctttgca tattgatgat
     8461 aaaaatcact tgccggatcg ggactgaatc gcccttgttc ctctaaaaag tcatcccaag
     8521 caacagcgcc tttgccacct tccttagtgg ctccttcaac ctgataggct gctgtggctc
     8581 cgccaaaaat aaaatcttct ggtaatttaa gcatgtacat ctctcctcaa atctataatc
     8641 agttttcgct ctttctaact gattgcattg taaacgcatc caaaacattt gtcaacaaaa
     8701 acaaacacaa ataatcataa aaaccagaaa acagcaataa aatctattgt aacaaacata
     8761 aaaaccaccc taaatgttta cttttgttcg aattagattg actgttgtcc gtttatatga
     8821 tagattatcc ttaaattaga ggtgaagaca atgttgaaaa aagaaagatt gcttactatc
     8881 gtagaaatgg tcaataaaaa aggaatcctt accgttaatg aaatcatcaa tcaattagat
     8941 gtttctgata tgaccgttcg cagagattta gatgaattag aaaaatcggg caagctgctg
     9001 cgtgtccatg gaggggctca aagtctttcc tacactttag atcaagaact ttctcacact
     9061 gaaaaagcca ctctgcagat tcaagaaaaa aaagaaattg ctgccacagc agcctccttg
     9121 attcacgagg gagaaacgat tttcttagga ccaggtacaa caatcgaact attggcgacg
     9181 catttactga acaagaaaat tcggatcatt acaaataatt atccagtttt tgatattctt
     9241 gctcaagcag atggaccaga aattctactg attggcggtg atttccggaa aaataccggc
     9301 gcatttgtcg gaccgatcac caacgacaat ttaagaagac taaagtttac aaaagcattt
     9361 atcagttcaa atggcgttca caacgaagcg atctctactt acagtaccga agaaggagaa
     9421 gcacagcaga tcgctctaaa taattcccgt aagaaatatc tactcgttga taataaaaaa
     9481 ttcaatcgag atgattttta tgtgttttat aatttgcatg atttcgatct gctcattacg
     9541 gatcgccaga tctcgcggga ggttcaagag cactatgcac aatatgtcga tctgcaaatt
     9601 gctgattcaa ccgaaaatac cactatagga gcgtaactat gaaagggatt atatttgatt
     9661 tcaacggaac aatgtttttg gactctcatc tgcatgaagc tgcttggctg catatgatac
     9721 atgatcatac aaaagatggt ttatcagacg aggatatttt aaaaaatatt catggtcgga
     9781 cgaatacgga gattttgaaa catttcattt cagagcattt gaccgcctct gagatcgccg
     9841 tattgtcaga agaaaaagaa agctattatc gtatgctttg cttgcaaaat gaaaacgagc
     9901 ttcagttgac aaaagggctt gaagcgactc ttgaccagct gactgaccaa cagattccat
     9961 taacgattgc cacggctact gtcaaagaga atgttgcttt ttattttgat attttcgatt
    10021 tagcccgctg gtttgactgg gacaagattg tctatgacga cggctctttc cctgggaaac
    10081 cgcaaccgga tatttttctt aaagcggcag acaaactgag cttagctccg gctgattgtc
    10141 ttgttatcga agatgcttac tcaggattac ttgctgccaa acgtgcgggg atcggaacaa
    10201 ttattgcaat tgatccattc ggtaaaaaca aaacagtatt tgaacaggca cagctaaata
    10261 aagatggaac catcaaagat tttactggtt tttggacaga gcacctgtct gccacatccg
    10321 ttaattctta aacgattcaa ctaactacca aagaaaagca actgaaatgc acacctcctt
    10381 ttataaaaaa ctgccagtga aacgacatga aagatacact gctatatcct tagtgaaata
    10441 tagcagtgta tctttgactt tttcattctt ttaaccatat gacgcgttca tctctatcta
    10501 taattaacat taaaaatagt gttttcacat tatttctcaa accaatctta aaaatcatca
    10561 ggagtacttt tttgatatac ttttattaat gctgggcgta atggttaaaa gatttccact
    10621 agttcctctc caccaagctg agttgaaaaa tgcttatctg taaacaacat agacaaagag
    10681 gaaataaacg tttccgtatc tttctctgtc ccaaggatat taattaattt tcctactagg
    10741 taagtatcat taaacttaag atctgccttc acatcaaaag ttttgataaa agcttttatc
    10801 atgcgtacaa ttttgtcttt atcgccataa ttgatttcac cactcatatc aatatagtca
    10861 ctaatccctg aagaaataat ggcaactgct tcttctttcg ccttatcaga taattcattt
    10921 aatgactggg attgattgat gacttcttga gttttatctg ttacgtaatc tctcattatc
    10981 ctatcggcat actccgcaaa gtaatcttga aaaaattctt ctacagggta accaacttcg
    11041 ttttcccaac caggagaaat ttcattatca gtacttatta aatctattaa aaaccaaccc
    11101 gttctctgag tgttgattct ataatatttt taatttccta tataaatcaa aaatattata
    11161 gaatcaataa gctaattaag aaacaaactt tagaaactct agaagaactt ctgaatttat
    11221 atagaagtgt gtagctactt actaaatata caaaagttaa aataaaatac tctggatact
    11281 ctttatactg cattaaagtt agtaatatta acagcaaaga tatcgaaaaa acaataacat
    11341 aatatgtgtc aaaatttttc ataataagaa ctcccttact gttgcatact aacataatcc
    11401 aaaaactctt ctacttcagc taaagacatt ccactttctt tagtccaaaa aacagttttt
    11461 tctatcgatc cttctctgta aactgtaaat gtcggagtcg agttaattga gtagcgactc
    11521 ttgaattctt tccactcatt ttcatcttct ctaacttttg ctacattcac gtaataaata
    11581 ttttcttttc ccttttctac cagcatatcg aatagcttcc tgcttttgat gatcgatttc
    11641 ttgtgctaca tctagtaaat tttcatcaat ttctatatca taaagtattt cactagtgtc
    11701 tacatccaca atatatactt tttatcagta gcaatttttg agtaatccgt taaaatgatc
    11761 tcagtctcgt cactttcatc agtataaata tacgttaaca atgaactcac cttttcaatc
    11821 ggtgttagct ctaagcctac tgtatttttt actattgagt cattatttac caccataact
    11881 tcgtactgta tataccatcc tatttggttt ccatcaatat cagtaattgg ctcactagct
    11941 aatggagtct ccggaacaat attttcctga tatttcttaa actcttctgt agcacgaaaa
    12001 aggttatcat gttcctcact tgaaactggt tgagtcatag aaaatgttac ttggctaaac
    12061 agagggaaaa taaacactac aatcaacaaa gtataaaaaa aagcccttct ttttcatttt
    12121 ttatcttcca atcacaaatt gaaaaactta ttcctgcaac tttgagtcta gtatcacaat
    12181 cgactgacta caactattca tttacattct actttaagtt cctccttaac cattaatttt
    12241 attaaagata cttgtaatag tgattctcac aaaatttcat gaaaaatact ttattactgt
    12301 atctaaaatt ccaatacaca acttatcgac tattatctga aaaccaaaac ttattattcc
    12361 ttgatgttat tctagcaata gtaactgctt ttttattttt ttgcatattt tttcggcgtt
    12421 actttttaaa gttaactttt tcgacaaata gaaagagata cgaatcactg atttttttga
    12481 agaaaagaaa tggagtgagc tgaacgttag agatattggg agctttcgta actgcttatt
    12541 aaacgataat ggttatttgc aaccgtattg ctcgcttcta catgaaatat ttcatgaaat
    12601 atttcgcaaa acctttgatt atgtagttac gtttccattc ttgactaaaa atgtttcaaa
    12661 aacgaacaaa ggtgttaaaa ggtaaagtga tcaatactac tggtcaaaat aagaggttga
    12721 aagggaattg aaaaataact acagataata cttttataaa cgtacgtgct ttgtggttct
    12781 gtagttaact atataactgg aattagggaa tggtatcaat ttgaaaagaa agattctacc
    12841 aatttatcat atgttaaaaa tggaaaatca acagcagttc tttcggaaac cttacacaaa
    12901 aaccgattat tgaaaatagc cgaaaaacgt ttttcggcta tttttttaat taagtttcct
    12961 tcttcttacc ttttcccact attccgtttc acatctttga tgatcttcag cgcacgtttt
    13021 cgtgttttga tatttgggct tttcaagcgg cggtatgcac ttgagatatc tgttttttcc
    13081 atatggacga cccactttct tctttgattg tagtcatgct aaaaaacaca aatcgaaaca
    13141 attacgattt gtgttttaaa tggtatcaaa agttaaaatg aaagtcaact tgttaaaaca
    13201 gtcgatcaga ctagctgatt gactcctatc tctggaaata aattttcatt attacgtttt
    13261 agctacctaa aaaaggccct cttgtccgtt aactttaata tctattgtta tagctagttt
    13321 acctattgaa cgtactgtgt gttcgataga acactaaaaa agaggctggg acagaagcgt
    13381 ttaactccaa gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc
    13441 agcttatttc cgaaggagtt gcttctgctt ccgccgttta tacgtgttta gagcgtgaga
    13501 caaaagtgtt ctttactttt gtctcacgct cttttaaact atttcgaagt actaaactta
    13561 tgaagtaaga ataccggctc tatacttttt gatgttggtg aacttcacca acatcttttt
    13621 tgta
//
LOCUS       DACDLI010000066        13146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDLI010000066 DACDLI010000000
VERSION     DACDLI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.066"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IV353_002091"
     tRNA            complement(77..149)
                     /locus_tag="IV353_002091"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IV353_002092"
     CDS             530..1099
                     /locus_tag="IV353_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9010298.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IV353_002093"
     CDS             1086..1946
                     /locus_tag="IV353_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9010299.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IV353_002094"
     CDS             2136..2840
                     /locus_tag="IV353_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9010300.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IV353_002095"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IV353_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9010301.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IV353_002096"
     CDS             4677..5993
                     /locus_tag="IV353_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010302.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IV353_002097"
     CDS             5994..6863
                     /locus_tag="IV353_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010303.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IV353_002098"
     CDS             6918..7727
                     /locus_tag="IV353_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9010304.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IV353_002099"
     CDS             complement(7830..8459)
                     /locus_tag="IV353_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9010305.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IV353_002100"
     CDS             8629..9921
                     /locus_tag="IV353_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9010306.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IV353_002101"
     CDS             complement(9997..10659)
                     /locus_tag="IV353_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9010307.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IV353_002102"
     CDS             10884..11168
                     /locus_tag="IV353_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9010308.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IV353_002103"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IV353_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9010309.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DACDLI010000067        13039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDLI010000067 DACDLI010000000
VERSION     DACDLI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.067"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            67..768
                     /locus_tag="IV353_002104"
     CDS             67..768
                     /locus_tag="IV353_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9010310.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            765..1886
                     /locus_tag="IV353_002105"
     CDS             765..1886
                     /locus_tag="IV353_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9010311.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1901..3133
                     /gene="pepT"
                     /locus_tag="IV353_002106"
     CDS             1901..3133
                     /gene="pepT"
                     /locus_tag="IV353_002106"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9010312.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3265..4173
                     /locus_tag="IV353_002107"
     CDS             3265..4173
                     /locus_tag="IV353_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9010313.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4208..4480
                     /locus_tag="IV353_002108"
     CDS             4208..4480
                     /locus_tag="IV353_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9010314.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4491..5537
                     /locus_tag="IV353_002109"
     CDS             4491..5537
                     /locus_tag="IV353_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9010315.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5788..8397
                     /gene="clpB"
                     /locus_tag="IV353_002110"
     CDS             5788..8397
                     /gene="clpB"
                     /locus_tag="IV353_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9010316.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8548..9219)
                     /locus_tag="IV353_002111"
     CDS             complement(8548..9219)
                     /locus_tag="IV353_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010317.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9212..9706)
                     /locus_tag="IV353_002112"
     CDS             complement(9212..9706)
                     /locus_tag="IV353_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9010318.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9726..10946)
                     /locus_tag="IV353_002113"
     CDS             complement(9726..10946)
                     /locus_tag="IV353_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9010319.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11093..12928
                     /gene="lepA"
                     /locus_tag="IV353_002114"
     CDS             11093..12928
                     /gene="lepA"
                     /locus_tag="IV353_002114"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9010320.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         4268 a         2384 c         2734 g         3653 t
ORIGIN      
        1 tttttttatt cctagtctcc ttattttatt tggaaaatgc tataattatc tagaaggagt
       61 gaagctatga accatttaga tttatctcag cgcttagccg cagtgggtga ctttgtccct
      121 gaatccgctc gtttagcaga cattggatca gatcacgctt acttacccgt tgcattgata
      181 ttaagaggaa aaatcgaata tgcagttgct ggtgaagtcg ttcaaggtcc ttatgaatca
      241 gcacgtaagc aagtcaaaaa agatggatta gaagaccgta tcaccgtacg ccttgcaaat
      301 ggtctagatg ctatccagcc acaagatggt attacagctg tcacgattgc gggtatggga
      361 ggttcactga tccgagatat cctagaagct ggcaagttga atcaacgctt aagcggacaa
      421 gaacgtttga tccttcagcc aaacatcgga gagcgtacat tacgatcttg gctgcaagaa
      481 aatcagtata aaatccaatc agaaaaaata cttgaagaaa ataaaaaaat atatgaaatc
      541 attgtagccg aaaaagaaca aaaaccagtt acttacaatg aaatggagct gatgttcggg
      601 ccatttctgc ttgaagagaa aagtgagatc ttcaaatcaa aatggacaag agagcttcaa
      661 caaagaaaag ctatccgtga acagctcaaa caagcagcta atcaagaggc acgtcaaaaa
      721 caaatcgagc aggaaatcca aatgattgag gaggttttaa agccatgaca atcagcggac
      781 gcactttcat ccaaaagttt gaaagctatt gtcctcagtg gctagctgaa gaaggagacc
      841 cagtcggact gcatatcgga acattggata agccaatcca acgagtaatg atgacattag
      901 acgttcgtcc agaagtggtc gaagaagcaa tcaagaaaaa aatcgatttg ctgattgcta
      961 aacatcctcc aatctttcgt cctgtaaaac ggctggttac tgatcagcca caagaaaaaa
     1021 tgtatgcaga tttgttgaaa catgatattg ccgtctatgc agcacacacg aacatggata
     1081 ttatttggga tggactgaat gactggtttt gtgaacttct cggtattgaa gtggagagtt
     1141 atttagtaaa gactcatgag attcattata aaaaactggc cgtttatgta cctgtggatc
     1201 atgcacagaa aatgcgtgaa gtattagctg cagctggtgc tggtacacaa ggtgattata
     1261 ccggtacaag ttttacctcg attggacacg gtcgatttac tccggaagct ggtgcacaac
     1321 cagcgattgg gaaagtaggg aagacagaac aagttcaaga ggcaaaagtt gaggttattt
     1381 tgccggaaac tatcgaaaaa caagtgattc aagccatgcg gtcagctcat ccttatgagg
     1441 aacctgctta tgatcttttc gctatagatg aaccagtcga aatgtttggg ttaggacgag
     1501 taggtgaatt gccgcaagaa atttctatag aggcatttgt agaacaagta aaagaagcgt
     1561 tccaactaga tggactgaga atagtccagc caaaaaatgc aaaatcctct gtgaagcgca
     1621 ttgcgatttg cggtggaagc ggtgaaaaat tttatccgca agcaatcgcc caaagagcag
     1681 atgtctatat cacgggagat atctattatc atacggctca tgatatgcaa agcgctgggt
     1741 tgattgcaat tgatcccggt cattatattg aatcgttatg caagcaacga tttatcgaga
     1801 aatttgaatc ttggaaacaa gaagaaaatt gggatattaa ctttttcgtt tctgaaacaa
     1861 atacaaaccc atttcaattt aattaaaagg agagaatact atgtacgaaa atcttttgcc
     1921 acgtttttta cgatatgtca aaacagaaac acgctccgat gcaacaagca aaacgactcc
     1981 atcgactcag acacaagttg cttttgcgca agaattgaaa aaagaattag aagagttagg
     2041 cttagctgat gttttctata atgaagataa cggattcgtt atgggaacat taccaagcaa
     2101 tgttgatcat gaagtgcgtt ccattggatt tatttcccac atggatacag cagattttaa
     2161 tgcagtagga gtcaatccac aaatcattga aaactatgat ggcgagtcta cgatcgtact
     2221 agatgcggaa gaacgctata cattgaatac aaaagatttt ccaaatttga aaaattatga
     2281 aggccacaca ttgatcacaa cagatggcag tacattactg ggagcagatg acaaatcagg
     2341 gattgcggaa atcatgactg caatggagat cttgatcaat aatccttcta tccctcacgg
     2401 taccatcaaa gtggcttttg gtcctgatga agaaatcggt gtcggagcgg ataaattcga
     2461 tgttgagcaa ttcgatgtag attttgctta tacagtcgat ggtggtcctt taggtgagtt
     2521 gcagtatgaa acgtttagtg ctgctcaagc aaatattacg atccaaggga aaaatgtaca
     2581 tccagggacg gcaaaagata caatgatcaa tgctctacag ttagcaatcg attttcataa
     2641 tcagcttcca gccgatgaag ttccagaaaa aacggaagga tacgaaggtt tcttccattt
     2701 gatggcattg gatgggaatc ctgaagaagc ttctatgagt tatatcattc gcgatcatga
     2761 tcgtgaaaaa ttcgaagcac gcaaagcaca aatcacacat atccaagaag agttgaatca
     2821 gcgatttgat caagaacgaa tcaaagtaga tatgtatgat caatattaca acatgaaaga
     2881 aatcattgaa aaagatatga gcatcgtgga attagcgaaa caagcaatga tcgagcttgg
     2941 tatctctcca gtgatcgaac cagttcgtgg aggaacagat ggttcaaaaa tctcttacat
     3001 gggtattccg acgccaaatc tatttgccgg aggagaaaat atgcacggac gattcgaatt
     3061 tgtttcttta caagtaatgg aaaaagcaac agacgtgatt gtaaaaattg cagaattgaa
     3121 cacaaagagt taaaatcgaa aaagaacagc ttatgacaag gcgttcgtcc caatgatgga
     3181 atgtcatctg tctaaggcta aagggcagga caatggaatt ggttgtcccg ccctcttttt
     3241 tgaagagtaa ataggaggta aagaatggaa aagctgctag ttttttataa caaaagttca
     3301 gggaaagatg agggtgagaa attagcacgc tggtttcaag attatgtaaa agaaaagcag
     3361 ccagaattat atgtagaact agctgaaact ggcccatcga ttgatgatgc ggaattagtc
     3421 aagaaagcag aagccataga agctgatact ttagtaatta taggtggaga cggcacgatt
     3481 catcatatcg tccaagcgtt ccaaaaaaca ttatctcaat accatgtcgg attacttcca
     3541 ggtggaactg tcaacaacct tgcacgtgtt cttgagattc ccctcaaaaa agaagaagct
     3601 gctgacgtga tcctggaagg caatgtacgg aaaatagact atggtcaagt caatgatgaa
     3661 gtcattatct ctacactgac aatcggaatc ttagcagata cagcagtttg gatcagtcaa
     3721 gaagaaaaac agcgttacgg ctcgtggatc tttatcaaga agttttttaa gttattgctg
     3781 aagaagaaaa aatatcattt ggatttagaa acagacgagg agcattggaa aggaaaatcc
     3841 caactgttga cagtcacgat gtctaactcc gtgggcggtt ttacaaattt tgacgactca
     3901 gcgaccctag atgatggaaa atttcatgta acgatcgtac cttccttaga tattttccga
     3961 tttatttttt atttgcctag aatcatgaga ggcaaattct atacgatccc cggcattact
     4021 tatttcacag ccaaacgcat gaaaatccaa gcgcaggaaa aatctgtggg cactcgaacg
     4081 gatggagaca cgacagatga tcttccagtt gaattatctg ttgtatcaaa aggattaaca
     4141 gtttattcac caaagaaaga agaaaaagct tagaaatagt agttaacaga aagttggaag
     4201 aagagtaatg gatgtattgt tacacaatcc tttgtggttt taccttttca ttgtgaattt
     4261 ttacttattt tgtctgatgg ggtatgataa acatcaggct agaactagcg gctggcgtgt
     4321 tcctgagaat aatttgatat ttgtcagcct gttaggcggt ggtttgggag gattgatctc
     4381 tcgaaaagtt ttccatcata aaactcggaa aaagaagttt actataggat tcgctgcagg
     4441 tgtacttgtt gatttgattc tcatttattt tttccattaa ggagcaggag atgtcgtcta
     4501 aaggaacgaa aactaaaatc attctgaatc tgcttttgct gattgtgatt tttgccatta
     4561 tttattattt aatcaatcaa tcatttgcag atatctttgc cgaattgctg tctaccagtg
     4621 ttgttgtttt ggcagcggct gttatattag ggacagttta ccaaatcgct gaagggcgct
     4681 caatcaaaga aattgctgaa cctttcgcgc cgaattttac aacgaaagat gggttcttga
     4741 cctctagcta tattgcgttt taccgaatcg tttcgtttgg tacaggaacg ctgatctcgg
     4801 agatttattt ttataacaaa aaaggaatga agctatccca gggagcaggg gtgacagcac
     4861 tccatatgat tatgtacaaa ctggcagtga tcacttatgc attagctggc ttggtcatcc
     4921 aattctctct tttttattct aatggtccat caatgatctg gtttatcatc gctggtatcc
     4981 ttttgacgat cgtgattgtt gcctttttat tggcgatgtc cgtcagcttg aatgttcaaa
     5041 tattttttgt cagcttatcg aataagttat ttaaaagtga gcgtttgcgg aaatgggtgg
     5101 atacatgcaa tcagcagatc tactctctaa gggaaacggt ccaaaccatt atccaagaca
     5161 ggactgcgct attgcggatt tatctatgga atatgttaaa acttgtattt tggtatgtac
     5221 ttccttattt gattctgatt gaaaaccatc ctaaaataga ttttctactg accatttcgt
     5281 ttatcagctt tgcagtagtg ctttcagggg ttattcctac gcctgctggt attggttcct
     5341 ttgaatttgt ctatttgtta ttattcagac cagttgttgg aacagttgac gcagtttctt
     5401 cgcttttact gtatcgtttc agttccttta tcttgccatt tctctatgga tttgtgtatg
     5461 tgattgctga acggcgaaga gtgatcaaag aagaaataat agaagtaaaa aaagagaata
     5521 aagaaaatca agggtaggtc ttttataaga tctacccttt ttatcaaatc aaagaaaaac
     5581 gttatggata agataatttt acgtttgttt ggctcttttt ttatatacat acaatcaatt
     5641 ttgttttttt aagatttata taagtcgaga aaaaaagtca aaaaactatg tgtatatttt
     5701 gaccaaaatt gaccaaagcg atataatagt ttttgtaaag atcaaagaaa taagtgatct
     5761 tacctttaat taggagggta tacctgtatg aatattgaga aaatgactac gacgcttcaa
     5821 gaagcgattg ctgaagctca acagattgcg gtcactcgaa aacatcaaga tatcgacatt
     5881 gcccatgtat ggaaaatctt tttacaatct aatcattttg gtcgaaattt ctatacggat
     5941 gccggattgg atgtcgaatc ttttgaacat gaaattgatc gtctactgga tgagtatcca
     6001 gttgtttctg gtggaaacgt ccaatatgga caaaacctca gccagaattt ctttcgatta
     6061 ttgaatgaag cagaccaaat acgtgaaagc ttcggcgacg agtttctttc aacggaagtc
     6121 gttattttag cgttgatgaa attaaaaaat tatccgttaa ctgtttattt gaataaaaac
     6181 ggattatctg aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg
     6241 acctcacaaa accaagaaga acaatacaaa gcattagaaa aatacggcgt tgatttagta
     6301 cagcaagtca aaagcggaaa aatggatcca atcatcggtc gtgatgaaga aatccgtgat
     6361 gtcatccgta ttctttcacg aaaaacaaaa aataatcctg ttctgatagg agaacccggt
     6421 gtagggaaaa cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct
     6481 gaaaatttaa aagataaaac gattttttca ttagatatgg gtgcattgat cgctggagca
     6541 aaattccgtg gtgaatttga agaaagacta aaagcagtct tgaaagaagt caaaaaaagt
     6601 gatggacgta tcctcttgtt catcgatgaa atccacaata tcgtaggggc aggaaaaaca
     6661 gaaggaagta tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat
     6721 ttgatcggtg ccacaacttt agatgagtac cgtcaatata tggaaaaaga caaagcgttg
     6781 gaacgtcgct ttcaaaaggt attagtcaaa gaaccaacag tggaagatac gatcagtatt
     6841 ctgcgtggat taaaagaacg ttttgagatc caccatggtg ttaacatcca tgacaacgca
     6901 ttagtagcag cagctacttt atctgaccgt tatattactg atcggttctt accggacaaa
     6961 gcaatcgatt tgatcgatga agccagtgca acgattcggg tagaaatgaa ctctatgcca
     7021 acggaattag atcaagttac tagacgtttg atgcagttgg aaatcgaaga agctgcactg
     7081 aaaaaagaat cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct
     7141 gaacttcgtg aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc
     7201 aattctgttt cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt agaagatgca
     7261 gaaaataact atgatttaga acgcgctgct gtattgcgtc atggtacgat acctcaatta
     7321 gaaaaagaat tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa gatggttcaa
     7381 gagtccgtta ccgaaaatga gattgctcaa gtcgttggca gattaacggg tatccccgta
     7441 acgaaacttg tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac acttcataaa
     7501 cgagtgattg gtcaagatga agcagttgat gcggtcagtg acgcggtaat tcgttcacgg
     7561 gctggattgc aagacccaaa tcgtcctcta ggttctttcc ttttcctagg accaactggt
     7621 gtcggaaaaa ccgaattagc gaaagcgtta gctgagaact tgtttgactc agaagatcat
     7681 atggtacgga tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga
     7741 gctcctccag gctatgtagg ttacgaagaa ggtggtcaat taacggaagc tgtcagacga
     7801 aatccttata caatcgtctt gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat
     7861 atcttgttac aagtcctaga tgacggacgc ttgactgatt caaaagggcg agtagtagat
     7921 ttcaaaaata cggttctgat catgaccagc aatatcggtt cacagttact gctggaaggc
     7981 gtgactgctg atggaacgat tccagaagca gtggctgaac aagtcaacac gctattacgt
     8041 ggaaacttca aaccagaatt cttgaaccga atcgacgaca cgattttatt tacaccattg
     8101 agcctagaca acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc acaacgtctg
     8161 gaacatcaag aaatcctgct gacaatcagt gatgaagcca aaacgtggat tgctgaaaat
     8221 gcttatgaac cagcatatgg tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa
     8281 acaccactag ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa agtaacgatt
     8341 actttacttg atggacaact tcatttcaaa acagaagagc tagaagaaat cgtctaatct
     8401 tccattgaga ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga cctccttttt
     8461 tcaaataaac agtgttccag aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc
     8521 tgcccgcttc tgtcacaacc tcttttatta ggtatttttc actttttcta atttataact
     8581 ttcaaagtaa cgctcatctt ttttactttg agaccatgca aacaatccca ttaaggcagc
     8641 acctatcgta tttgtcagca agtcgcccat cgtatccatc aatgctgctc gtccgacaaa
     8701 cagtgtgcca tcagaagcag caaaacgttg aagattcatg cctaagaact ggtcgcataa
     8761 gaattcccag aactcccaaa acacgccgca aagtccagca aacgcaaagc cgaataagag
     8821 gaacaaccaa ggactggttt ttgatatctc agcatctttc atcaagtaac caatcaatcc
     8881 atagccaaga gctgtcagga ccattggact caccgcatgt aagattttgt cccaaaaaga
     8941 gatgatactg atcaaatgca tccctgtgcc taaaaagaca gagatgaaca agaaaaacca
     9001 gtaaaacaaa acgattgctg ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg
     9061 catgaaaatc aatgcaatcc ctaataaaga ttccacgata aatgtgattc cttgagaagt
     9121 cgaatatttt tgtgtgataa attcataaaa attatagcag acaacgaata caccaaaagc
     9181 aagtaaagct atttgcacct ttttgatttc tttaatcact gaaagtccct ccatctaaaa
     9241 atctttttac ttctgaaagc aactcaattt ctgaagaaac cacttttccg ttcaatttga
     9301 tcaaacctat tgtatataag ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg
     9361 cagcgatttt tttcgggtgg gttccgcctt gctgtcgagt atcagtatat aagccgatca
     9421 ctgggatgcc tttcgcataa gccacaccga tttccgatgc taccccggca tcgatcgtca
     9481 gcccatctag taaagcaatc attaaatcac tagccaacac tttttctgtg tcagcaagag
     9541 caatcattcg actgtctgca taagcagttt tgtcattgat tgcttcattt tcttgaggaa
     9601 gatagatcga aagctcagaa gagagctctc tgatttcttt aactaactgt tggttatata
     9661 acaaatcact tttagcaaac attggtgcgg caaaataaat tttcatctaa acatcatcct
     9721 ttttatcaaa aataatcgct aaagtaagct tccatgtttg gaatgatacg ctctagtgac
     9781 ttcagtgttt cttcatctgt ttctaaccgt tcgattcttg ccaaataaga agcacggcga
     9841 taattcatca tcaagcaagt caatgtttgg atatccagtc gaacggattt tcctataggt
     9901 tcatttaaga cacggacttg tccctgttca tcccatgtta aagcgaagac accattattc
     9961 caaggagcga caggatcttc aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga
    10021 aaccgttgaa ggaattcttt tacatcaacg atccgagcca taaaatatgg ttcgatctgc
    10081 tctttgatct gactatcttc aatcaaaaat gctaaaggct catttttata aatatctcca
    10141 tgtacccagt agatcatgga aaaatgtgcg gaaataaagt tccataagcc atttcgagct
    10201 tcttgatcaa gatagaacat ttcttttacg tgaaatattt cttcagctac ccagtagaac
    10261 agaacacctc ttggttcatg gttgctatcg tagtagacag cggctgtgcg ctcatcttca
    10321 ttttcgaaac gccaatactc ttcccaatga aaatcactgc ggaataaagc accatggttt
    10381 tgttgggcaa atttgtcata aacggaaaat acgtctggat gatcaactgg taatcgctca
    10441 accataccac ttactgggat ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt
    10501 ttatcagaca tgatttccca tcctttacgc cgatagtagg ggatactgta aggatacaaa
    10561 taagagatcc attgcttatt cttgcgcata ttatccaatg caacgataat tagatcttgc
    10621 atcaatccat ggttagcata ttctggataa gttccaactc cagtcacacc acccatttta
    10681 taacgtgcac cgtgaatatt gacttcacaa ggatagatag cgatttgaga gatcagttta
    10741 ttttcgtgga accatccgaa cactttcgat acttccaaaa tcggtttttt ggaacggata
    10801 aaagctcgtt tattctcaaa cccgctttcc tcgatgtctg cttcagtcac ttggaacaca
    10861 taggaaagca attcattgaa ttgatcgata tgttcttgag tgaccggttt taattcaaga
    10921 ttttctctaa aatctttttg atccattttt tcatctccat ttattagctt gataacagta
    10981 tagcaaaatt ctacaaaaaa aacttctata gctgactaat taaaaactct ttaaattact
    11041 gcttttcgtt ggtataatgg aaagtgctga ttatatgaaa agaggagacg ctatggatat
    11101 aaagaaaatg aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg ctcatatcga
    11161 ccatgggaaa tctacattgg ccgatcgtat tttagaaatg acgcacactg tgacgtctcg
    11221 cgaaatgcaa gatcagctgt tagactcgat ggatttggaa cgtgaacgtg gaatcacgat
    11281 taaattaaat gctgtagaac tcaattacac agcgaaaaat ggagaaacat atatctttca
    11341 cttgattgac accccagggc acgtcgactt cacctatgaa gtctctcgaa gccttgctgc
    11401 ctgtgagggt gccgttttag tggtcgatgc agcacaagga atcgaagcac aaacattggc
    11461 caatgtttat ttagcgttag acaatgattt ggagatcctg ccagtcatca ataaaatcga
    11521 cctacccgca gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga
    11581 tgctagtgaa gctgtattag ccagtgccaa atcagggatt gggattgaag atattctcga
    11641 gcagatcgtg gaatatgttc ctgcaccaag tggagactta gatgcaccgc taaaagcttt
    11701 gatctttgac tctatttatg atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga
    11761 cggtatggtc aaacccggag acaaaatcaa actgatgagt aatggcaaaa catttgatgt
    11821 tacagaggtc ggggtcttct cgccaaaagc agttgctaga gactttttga tggtcggtga
    11881 tgtcggttat atcacagcaa gtatcaaaac tgtccaagat acacgagtag gggatacagt
    11941 aacgttagca gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat
    12001 ggtttattgt ggtctttatc caattgatac ttctcgttac aacgatttgc gggaagcttt
    12061 agaaaaactg caattgaatg atgctgcttt acaattcgaa ccagaaacct ctcaagcatt
    12121 aggttttggg ttccgctgtg gattcctcgg acttcttcat atggatgtcg tccaagaacg
    12181 cttggaacgg gaattcaatc tagaattgat cacgactgca ccatccgtta tttaccatgt
    12241 taataaaacg gatggtacga ctgctacagt agataacccg gcagattttc ctgaacctgt
    12301 aacgattcag gatgtagaag aaccattcgt caaagcacag atcatggtcc caaatgattt
    12361 tgtcggtgca gtaatggaac tttctcaacg aaaacgggga gaattcatta cgatggatta
    12421 tttagatgat taccgagtaa atgtcgtcta taatattccg ctttctgaaa ttgtctttga
    12481 ctttttcgac aaattgaaat caagcacgaa aggctatgcc tcattggatt atgaaatgtc
    12541 tggttatcaa aaaagcaagt tagtgaagat ggacatcttg ctgaatggag aaaaagtcga
    12601 tgcattaagc tttatcgttc accgagactt tgcctatgaa cgtggaaaag caatcgttga
    12661 aaaactgaaa aaactaatcc cacgccaaca atttgaggtg ccaatccaag cagctattgg
    12721 acaaaaaatc gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct tggctaaatg
    12781 ttatggtgga gatgtttctc gtaaacgtaa actcttagag aaacaaaaag aagggaagaa
    12841 gcggatgaaa caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa
    12901 aatggacgag gacgaaccga agaaatagca agggtttgtt tgaaaacagt gtaaaaaata
    12961 aaccatgttt acatggattt gccaccggtt tgccaccggt ggtttttttc ggtggcaaaa
    13021 atcaagaatt ctttctaag
//
LOCUS       DACDLI010000068        12951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDLI010000068 DACDLI010000000
VERSION     DACDLI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.068"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            139..477
                     /locus_tag="IV353_002115"
     CDS             139..477
                     /locus_tag="IV353_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010321.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="IV353_002116"
     CDS             complement(682..1257)
                     /locus_tag="IV353_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9010322.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="IV353_002117"
     CDS             complement(1989..2570)
                     /locus_tag="IV353_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9010323.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="IV353_002118"
     CDS             complement(2753..3379)
                     /locus_tag="IV353_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9010324.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="IV353_002119"
     CDS             complement(3401..4732)
                     /locus_tag="IV353_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9010325.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="IV353_002120"
     CDS             complement(4887..5288)
                     /locus_tag="IV353_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9010326.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="IV353_002121"
     CDS             complement(5374..5580)
                     /locus_tag="IV353_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010327.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="IV353_002122"
     CDS             complement(5675..7177)
                     /locus_tag="IV353_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9010328.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IV353_002123"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IV353_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9010329.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="IV353_002124"
     CDS             complement(7748..8365)
                     /locus_tag="IV353_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010330.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="IV353_002125"
     CDS             8586..9959
                     /locus_tag="IV353_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9010331.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="IV353_002126"
     CDS             10034..11146
                     /locus_tag="IV353_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9010332.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="IV353_002127"
     CDS             complement(11218..12855)
                     /locus_tag="IV353_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9010333.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         3503 a         2748 c         2311 g         4389 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgaccct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt t
//
LOCUS       DACDLI010000069        12950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDLI010000069 DACDLI010000000
VERSION     DACDLI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.069"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            102..545
                     /locus_tag="IV353_002128"
     CDS             102..545
                     /locus_tag="IV353_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9010334.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(551..1735)
                     /locus_tag="IV353_002129"
     CDS             complement(551..1735)
                     /locus_tag="IV353_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9010335.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2003..2701
                     /locus_tag="IV353_002130"
     CDS             2003..2701
                     /locus_tag="IV353_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9010336.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2708..3385
                     /gene="nth"
                     /locus_tag="IV353_002131"
     CDS             2708..3385
                     /gene="nth"
                     /locus_tag="IV353_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9010337.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3511..4044)
                     /locus_tag="IV353_002132"
     CDS             complement(3511..4044)
                     /locus_tag="IV353_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010338.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4341..6722)
                     /locus_tag="IV353_002133"
     CDS             complement(4341..6722)
                     /locus_tag="IV353_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9010339.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6712..7359)
                     /gene="recU"
                     /locus_tag="IV353_002134"
     CDS             complement(6712..7359)
                     /gene="recU"
                     /locus_tag="IV353_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9010340.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7423..7962
                     /locus_tag="IV353_002135"
     CDS             7423..7962
                     /locus_tag="IV353_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9010341.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8052..8471
                     /gene="gpsB"
                     /locus_tag="IV353_002136"
     CDS             8052..8471
                     /gene="gpsB"
                     /locus_tag="IV353_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9010342.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8493..8860
                     /gene="rnpB"
                     /locus_tag="IV353_002137"
     ncRNA           8493..8860
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV353_002137"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9069..10235
                     /locus_tag="IV353_002138"
     CDS             9069..10235
                     /locus_tag="IV353_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9010343.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10281..11777
                     /locus_tag="IV353_002139"
     CDS             10281..11777
                     /locus_tag="IV353_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9010344.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11858..12541
                     /locus_tag="IV353_002140"
     CDS             11858..12541
                     /locus_tag="IV353_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9010345.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4354 a         2252 c         2664 g         3680 t
ORIGIN      
        1 ttttttcttg gtttcagatt gggttttcct acaaaatatt tttatctttg ttgaatagga
       61 aacaaaaaaa caatataatg aaagaaaaag aaggtgtaga aatggataaa agacaactgc
      121 taatagaaga atatctggat ttagctaatg agattattca tcgtaataaa ccagaaatgg
      181 aaaaagaatt tgcaggattt actctaaatg aactagaagt gattgaacat atcgggaaaa
      241 tccctgatcc taacgtgaca aaattagcga atgctagtta catgactaga ggagccatca
      301 gtaagctaac aaaaaaactg atagctagag ggatcataaa cacttatcaa agcgaagaga
      361 ataaaaaaga aatctatttt caattgacgc caatcggaca aaaaataagg aatagacacg
      421 agcaactcca tcaaaaatgg gtacagcgag atgctactgt atttgaaaat atgtcaaaag
      481 aagaatttga cacagtattt cgttttatcg gtcgatacag agaatttata cgaagtagca
      541 aatgaagcta ctatttttta ttgtagtgat aataggcaaa aatcataagc aaaaatccga
      601 ataccgtcgg aagaaatggt gctgcagcat aaagctgtcc tgcgattagc gggccgatga
      661 cgcgcgatag agattgtata ctttgggtac cgcccattac aagaccttgc tggttatctg
      721 agacagaatt ggatagttgg ccattgaata caggagtaaa aatagaatca ccgaaaccat
      781 aacagataat cccaaataaa aacaaaatcg gcagtagaag aattccagat aatgtgatga
      841 atagataggc gaggatttca ctgaacatcc ctattcttgt gatctgctgt tctgaaaatt
      901 ttttgagcaa tcgaggcata atgaacaatt gcgagacagt atccaagatt ccaatcaaag
      961 agaagcttaa accaaccaac ccagccttcc actgaaaggc gtcaatcgaa aattgagaaa
     1021 agatagattg aagagagcca gctgccaacc aaacagcaaa acctgctgtc aataaaggaa
     1081 taacggatga aatcttgaat aattgttgta attggtgaaa aggtcttaca tgggagaaac
     1141 ttaaatctac tgaacgtttt tttattggaa ggctttctag cataaatgtg tagccataaa
     1201 cggcatttaa gaaagtaaag agtgctccaa taaatatagg aacagaattc cccaactcag
     1261 ctaatagacc ccctataata ggacccagcg ttgttcctat accgactaaa gcacccatcc
     1321 atccaaaaac tttggttctt tcattaggtt ccgtgatgtc ggcaaagtaa gcatataagg
     1381 tggagatttc tcctgccgtc aatccttcaa tgatacgacc aaggaacaac atccatatcg
     1441 aatttcctaa accaaaaagc aaatatccga tacttgatcc taataaactg ataataagga
     1501 tcggatgacg cccaaaatga tcacttaagc taccgagaat gggggcagat aagaaggcag
     1561 ctagagcata aacagacatg agcaaagtga tatagaaagc ttgctgatgt atggagtgag
     1621 aataaggcag cgttaaaaac ggaagtacag gactgacgat agtaaatcct aatccagtga
     1681 ataagacaga gacgtatcca aaaactttag aatgtttatt tgtgaaactg tacatgtaaa
     1741 aactccttta tgcaataatg ttaacttgga aacaaaaata gtgtatagta attttgtttc
     1801 cttgtcaaca ttctaaggaa cagcgaaatc tacttaattt atttattccg aaaagtgaag
     1861 atacgcatca aaaacatgaa caaacaaaaa tcaagtagac gacattgtaa atctgaccga
     1921 tcagtgaagt aaatagaaaa gaaacctgtt cttgcttccc atgttttttt tacgtatact
     1981 gtgattgagc ggaggagaaa tcatgttgaa tttagaaaat tacttaagcg ctggacaaac
     2041 agtcatatct aatcttttac ttaaaaatta tcataggatc ggtctacaat ctgaagaatt
     2101 tttatttgtt ctacagttgc atatggcaca gcttgaagga gatactttcc ctgatttgca
     2161 gatgattgct atggatatgg gaatcaaaca agatcagatt tttcaaatac tggatcgtct
     2221 cgtaacaaat ggatttatca agatagaaac gacgattgtc aacggtaaaa aagcagatcg
     2281 atataatctt taccccatct atgatctatt aggtgagtat ttaaaaacac aagaaaaaaa
     2341 atacgaagaa aaaacacaag aaaaagaaat ccagtcagtg tatcaattat ttgaacaaga
     2401 atttggacgc ccgctttctt cgatcgagtt ccagcggatc ggacaatggc tagaagaaga
     2461 ccattatcag ccggaaattt tgaagctagc actgcgggaa gccgtattaa atcaggctta
     2521 tagttttaat tacgttgacc gcattttgct atcgtgggaa agaaaaaatc tacgtacaaa
     2581 acaacaagtg gaagaagacc aaaaacgaag aaaacagcag cttcttcaaa gagaagtaga
     2641 acagtcagct caacaagaag aattgccgaa agtctcactg aaaaattggt tggaggagta
     2701 gcggaatatg ttaagcaaac aaaaaacaat ggaagctcta gaaacgatgt atggaatgtt
     2761 tcccgaagca catggagaac tcaagcacaa taatccattt gagttattga ttgcagtgat
     2821 cttaagtgcc caggcgacag atgtatcagt gaataaagca acgccagatt tgtttgcctc
     2881 ttttccgaca cccgatgcgt tagcagaagc ttcaatcgat gaaatcattc ttaaaataaa
     2941 aacgattggt ttgtatcgaa ataaagcaaa aaacatcaaa gcgtgtgcac agcagctgat
     3001 tgaacgtttc gatgggcaag tgccaacgtc aagagaagaa ttgatgtctc ttcctggtgt
     3061 aggcagaaag acagcaaatg tcgtattggg ggatgcattc gggataccag caatcgcagt
     3121 agatacccac gtggaacgtg tctcaaaacg cctgaggatc tgcaaactag atgctacggt
     3181 catggaagta gaagaaacac tgatgcggaa agtaccacaa gaattatggg taaagacaca
     3241 tcatacactg attttttttg gtagatatca ttgcacggca agaaatccca agtgcgaggt
     3301 atgtcctttg ctatcaatat gtcaagatgg gaaaaatcgg atgcgtctaa aagaaaaggc
     3361 gctaaaaaag aaaccacgcc tgtagacgtg gtttctttaa ttgcaaataa atgtatgaat
     3421 tatcttgtta ttatggagaa aagctgtttc gtttatcttt tttgcatgaa aaagtaaatg
     3481 tacatactca gtagctagga tggtaactta ttacaattta ttgcgataaa aataaaagtt
     3541 gttattttta ttgggaaaat acccgatgta gaaaataatg ccttccaata tacctataaa
     3601 ataagtccag tattccaaag gattatataa ataagtgacg tatctatcta aaggtgtaat
     3661 aaaaatgata agtgataata aaaaaatacc atttataact aaacataaac taatggattt
     3721 gggcggtttc aggctcatca gaaaaatata gattctggtg aacaacaacc ataaaaaagt
     3781 gagaatcatt gcatgagagc ttctttccat aaattttcct ttaagagtag tgcttaaaag
     3841 tacagaaaaa ataataaatg caaggatagg agcaattaaa gtaaatttat ttaataataa
     3901 acaaaatttt tcagcttttt tattcttgga ataacgaggt attgttttcg ataaaaaaag
     3961 atggtgcaga caaattatga taattaagca tactatcaga taactcaaaa tgaagccttc
     4021 tttcaaaaaa ttattcaaaa ccatatattg tattttttat ttgataataa caatgggatt
     4081 attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa ttataaacaa
     4141 gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat ttttaagcta
     4201 aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt aatacagctt
     4261 ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa accacgcttg
     4321 tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct ggatggtgta
     4381 tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc aggaggagca
     4441 gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt tgaagacgct
     4501 ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga ttcgctttga
     4561 ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg agtctggaca
     4621 tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc tgtatattga
     4681 tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc ccagtctgta
     4741 tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga ggctacgtgt
     4801 gacgattctg aagtgacagg tgtcattttt ttattgtaac ctgtccacac ggaaatcgaa
     4861 taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga aataccaagt
     4921 tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg atataaccca
     4981 gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat gtctgtgatc
     5041 atataagctg tttctggaga catggctgtt tttccatctg gctcataagt ttcttctgtt
     5101 ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc tccattggcg
     5161 aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt cccatcttgt
     5221 tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc gatcccaaga
     5281 ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt gactgctgga
     5341 acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata ttgattatcc
     5401 cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt aatagttttt
     5461 ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta
     5521 gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac gtcttctgcg
     5581 atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat aagggtggag
     5641 gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac gatatcatat
     5701 agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc tgtgtagaca
     5761 ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt atcgacgatc
     5821 ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg tacattaaca
     5881 gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata caaaacagta
     5941 tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga aggggcttga
     6001 ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt cttaccaaag
     6061 tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac tttatttacg
     6121 tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc catccacgct
     6181 tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa ggaaagtttg
     6241 atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa gttagagagg
     6301 gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct
     6361 accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat tttttcacgc
     6421 ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt ggaagatacg
     6481 gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt
     6541 cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag gatcttgata
     6601 aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga attcttcttc
     6661 acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc
     6721 attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg cgtccaaata
     6781 aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga tcaaagataa
     6841 aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca aatgaacact
     6901 attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc agaccccatt
     6961 ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt taaagggaaa
     7021 agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc gatagacgcc
     7081 attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag cagcacttct
     7141 cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga taaccgccaa
     7201 cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc gcatcccccg
     7261 attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg gagaggcatt
     7321 cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca ctcctaggcc
     7381 acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa gtcaaaagta
     7441 atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa
     7501 atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga agaagggctg
     7561 gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca aatagtagcg
     7621 gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt ttctgatttt
     7681 ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca attagcagat
     7741 tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa gaatcacact
     7801 cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga attccctgga
     7861 aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc ctacgaaatt
     7921 cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt
     7981 tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta
     8041 gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa caagaattca
     8101 aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac aatatcatca
     8161 aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac gatcgattga
     8221 gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa
     8281 cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc ttgtcaaatc
     8341 ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac
     8401 aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat gaaaaaacac
     8461 gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg tctgacttga
     8521 tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc
     8581 gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta aggcttcggc
     8641 tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa cccatccggt
     8701 ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg
     8761 gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg ctttgttttt
     8821 tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac atcagggaat
     8881 taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa agctattttt
     8941 caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc ggttattcgg
     9001 aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta taaataagga
     9061 gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca agcgggctag
     9121 aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt gagaacggac
     9181 gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg ttacgtacag
     9241 cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat gaattgtttg
     9301 aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct tttcctgtag
     9361 ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg atcacaaaaa
     9421 aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta ccactgacgg
     9481 aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg atggtaactc
     9541 tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa ggtggagcac
     9601 cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga aaagaccgtc
     9661 cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc ttaataggcc
     9721 ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat tggttcagtc
     9781 aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat gatattgaat
     9841 tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa gccaatgcag
     9901 aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa
     9961 cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt ctaggagaag
    10021 aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca ttaaaaacat
    10081 ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga caaaaagcaa
    10141 ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag tattggggaa
    10201 ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt cattcaattg
    10261 gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta aaagaaatca
    10321 atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt atgcctgaaa
    10381 aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat ttttcaaaaa
    10441 aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg gaagaattgt
    10501 cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg gaacatcgcg
    10561 taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac ggacaatggc
    10621 aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat caaaatattg
    10681 aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat tatgatgttt
    10741 tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta tttgatcagc
    10801 ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact gaacctgaaa
    10861 cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa
    10921 tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc cacccattta
    10981 tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa acagatttca
    11041 aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa caagacatcg
    11101 atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc catgaatcac
    11161 aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa aagcaatatc
    11221 ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac aaattttatc
    11281 gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc
    11341 ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg
    11401 ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga atacgaccaa
    11461 caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc tttggttatt
    11521 ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct ttgcagcaag
    11581 aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa aactggatgt
    11641 cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa
    11701 cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg tattttaatg
    11761 tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct aatctataga
    11821 tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac aagtgatcgc
    11881 tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg atcataccaa
    11941 aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag accgctttgt
    12001 gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta aaaatgagaa
    12061 cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag aggaacagat
    12121 ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct ctgaagcggc
    12181 cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag cattaggagc
    12241 aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga tttttgatcc
    12301 tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca cgacagtgat
    12361 caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg attacggata
    12421 tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat tttttctgga
    12481 atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata acaaaaaatg
    12541 atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt
    12601 tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa
    12661 ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa
    12721 ttggcaggag aacaccgttt attcggaaga ataggactgt gacaagactt ttgtcacagt
    12781 ccttttttcg aattcaacaa gtaaaacttt taagaaatat tcagagagta aaattaaaaa
    12841 ttgctgataa acgaagatgg aaattttatg tatttttatt ggtctatgca ataaataaat
    12901 taaatattca gaatacaatt gattttaacg aaagggcttc tatcaatggt
//
LOCUS       DACDLI010000070        12851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDLI010000070 DACDLI010000000
VERSION     DACDLI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.070"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..258)
                     /locus_tag="IV353_002141"
     CDS             complement(<1..258)
                     /locus_tag="IV353_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9010346.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRT"
     gene            complement(260..457)
                     /locus_tag="IV353_002142"
     CDS             complement(260..457)
                     /locus_tag="IV353_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9010347.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(851..2578)
                     /locus_tag="IV353_002143"
     CDS             complement(851..2578)
                     /locus_tag="IV353_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9010348.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2571..3764)
                     /locus_tag="IV353_002144"
     CDS             complement(2571..3764)
                     /locus_tag="IV353_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9010349.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3951..4595)
                     /locus_tag="IV353_002145"
     CDS             complement(3951..4595)
                     /locus_tag="IV353_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9010350.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4689..4823)
                     /locus_tag="IV353_002146"
     CDS             complement(4689..4823)
                     /locus_tag="IV353_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9010351.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4825..6957)
                     /gene="feoB"
                     /locus_tag="IV353_002147"
     CDS             complement(4825..6957)
                     /gene="feoB"
                     /locus_tag="IV353_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9010352.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6954..7427)
                     /locus_tag="IV353_002148"
     CDS             complement(6954..7427)
                     /locus_tag="IV353_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9010353.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7828..8052
                     /gene="nrdH"
                     /locus_tag="IV353_002149"
     CDS             7828..8052
                     /gene="nrdH"
                     /locus_tag="IV353_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9010354.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8211..10370
                     /gene="nrdE"
                     /locus_tag="IV353_002150"
     CDS             8211..10370
                     /gene="nrdE"
                     /locus_tag="IV353_002150"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9010355.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10420..11385
                     /gene="nrdF"
                     /locus_tag="IV353_002151"
     CDS             10420..11385
                     /gene="nrdF"
                     /locus_tag="IV353_002151"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9010356.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11474..12613)
                     /locus_tag="IV353_002152"
     CDS             complement(11474..12613)
                     /locus_tag="IV353_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9010357.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
BASE COUNT         3965 a         2636 c         2212 g         4038 t
ORIGIN      
        1 agttcttcca atttcactca aatttcttat taattcctct tgatattcag tgaatcttat
       61 tttattagta taagcagtaa aatatagata attcaccaat ctatttatta aaggactggg
      121 atctaattca tatttttgtt tttctaattt taaatatacc ttttgtaaaa cctcaagaat
      181 atcagaatat ttaggatctt tacttataga tatatcatta tataattcat gaacaatttg
      241 cttagcattt tttttcattt tagcacttcc ctggaattgc tccacctaaa aaaccaccta
      301 tagacattcc tgcaatacaa gtagttgcct tggcccaatc gaccgtacat ttatttttag
      361 tgcaatacac tccatttcca taatattttc cactatgagt ggtaccccca tagataagtt
      421 gtgtctcttt aatagacaaa atttttaaat gtttcataat ctaatcaccc ctaaattaat
      481 gtttttagac tattattcta acattcatta aatttcaaaa aacaattcct tcttgaaacg
      541 tagcaaaata attcctgaat aagaatttaa tcacttttaa gtatataaac tatgtatatt
      601 ctttaatatt ttttgagaat gcacttcgta cataataaac tttaatcaca ttagtctttc
      661 atacctacga tcgatctctt tgactcgcac ccataaattc ataatagttt gtcggcgtct
      721 ttgaacgttt cgaagatgag ctacgcattc tcttttttta tagacaaaaa agatagagac
      781 gaacatggag cttccaaatt tggatatttg atccaatttt ggaagctcca tttatttcgt
      841 gtctgtcttt ttattttttc cagctttcga ctaattcttg gttctctttg atccagtctt
      901 ttgctgcttg ctgtggatct tttccattgt tgatttccaa cataactttt tccatatctt
      961 tttctgacca atggaaattg tctaatactt tgtaagcttc tggctggtct tctttcaatc
     1021 cttttcgaac catcgtatgg atctgctctt cgcttcccat tccgttttca ggatcttcta
     1081 agtatttcag atcatatttg gcaaacatcc aatgaggtgt ccatcctgta actacaattg
     1141 gttcgtgttt tttgatcgct tgacctagtg caacggtcat tgctcctgaa gaagatgtct
     1201 ctactttcca gtctttcaag ttgtcatatt tttggatcgt gttttcagca gccgtaacta
     1261 caccagctcc tggctcgatt cctatgattt tcttacctgc ctgatcggtt aactcatcga
     1321 tcgaatttac atccatgtag gctggcactg ctaaaccaac ttttgctcct gtcaaattgg
     1381 cacctaggtc ttcgacttga tctttatatt gtgcatattg tgaaccatga gttttaggta
     1441 gccaagctga aaccatggca tctgactcgc catttgaaac agatttccac atgattgaat
     1501 tatctaatgg cgtcatcgtt acatcatagc ccatttgttt caatacttca ccgactacat
     1561 ttgttgaagc aacttctgta tcccattcaa cataagataa gttgatacgt tttgtttctt
     1621 tggcagatga aaaactccct ataccaatcg cacctaagat cactactact acagcaccaa
     1681 tgatcagtcc ttgacgtttt ttacttttct gtgttgttgc tcctgcagct tttttattgt
     1741 ttggctggtt caaatgctgt gtaaaacggt caatgatgat tgctaagata actaatgcaa
     1801 ccccgttgac aaatccattc ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc
     1861 ctaagccggg tgcaccgatc atagaggcaa taacgaccat tgaaagtgac agcattgttg
     1921 tttgattgat ccctgccatg atcgtacttt tagctaaagg caattctagt ttgaatagtt
     1981 tttgacgact tgtgctcccg aatgaatcag aagcttctac taattcttta ggaacttgtc
     2041 gaatccctaa attcgtaaaa cgaacggttg gtggcagagc aaagatgact gaggcaaata
     2101 cccctggaac catcccgata ccgaaaaatg caactgctgg aatcaaataa acgaatcctg
     2161 gcattgtctg cataaagtca aggatcggtt tgataatact ttgggcttta ctgctttttg
     2221 ccatcaagat ccctaatggt accccaatga tgatcgagat cacactagat aaaagaacaa
     2281 gtgtgacagt attcatcaaa tcattccata gaccttggtt ataaataaac catagaccga
     2341 ttaatgtgaa caacgtaagt cctggacgtt tcttagaaat aaagaaagct gctaccgtca
     2401 ataacaagat aaacaaagga gctggaatca ctaacagtaa atttgtaatc cccgacatca
     2461 ctgcctgtcc aatcgtttgt aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt
     2521 ctgtgatatt ttctacccag tttgccactg gtaattgata gttcaataga ttattcatgt
     2581 tcactcacct cgcttgtttc tgctaatgct tcaatcacac tacctcggat gacgacacct
     2641 tttaattttc catcctctac aactgcaagt ggagtcggtg agtcataaat aatattgaaa
     2701 atatcactta ccaccatgtc tttcccaatc accgtcacat tttcatctag gaagtcgatc
     2761 aatggacgtt tttgcttcct agcttctaac gcttgatctg cagtgatgat ccctttcagg
     2821 tgtctctttc tgtcaacagc catcagcatg ctgacttctt cattacgcat ccttgtcaat
     2881 gcaacgtttg gtccatcaga ttcgatattc gtcgtcaatg caggtaccat gatgttttga
     2941 gccgtaagca ctttggaacg atcgacttct tccacaaatt cacgtacata atcatttgct
     3001 ggattggtca atatttcttc acctgttccg atctgcatga tttcgccgtc tttcatcaag
     3061 gcgattctgt ccccaatccg taaagcttca ttcaaatcat gggtaatgaa aataatcgtt
     3121 ttttgaacat tggcttgcag gtccagtaat tcgtcttgca tttctcgccg gatcagcgga
     3181 tcaagagccg agaacgcttc gtccatcagc aagatctcag gatcatttgc taaagctctg
     3241 gctaggccaa cccgttgttg catcccacca gataactgac taggatattg gtctttaaat
     3301 gaaagcaaac tagaattttc taatgctttt tctgcttttt cttgtcgttc ttctttaggt
     3361 acaccacgga cttctaaccc gtattctgta ttttccaaga tcgttcgatg tggaaacaga
     3421 ccgaagtttt ggaaaaccat attgatttta tgacgacgga cttctcgcaa ttcttctttg
     3481 cttaacttcg atacatcttc tccatcaata tagatactgc ccgaagttgg ctcaatcaga
     3541 cgattcaata gacggatcaa tgtggatttc ccactcccgg aaagccccat gatcacgaag
     3601 atttctcctt cttttacgtc aaaattcacg tcatacacac caaccgttgc accggttttc
     3661 tttaaaatct ccgttttcgg ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt
     3721 ttaccaaata tctttgttaa atgttttact tctactttta ccaaacaaat tctccttttt
     3781 tatgtaaatt catttttctt tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc
     3841 actttgtcaa aaatgaaaga aatttgcttg tccaaactga aaaaaacttg gctaaatctt
     3901 tgtaaataaa aggtttagcc aagttaatat ttttttaata tttcacacaa tcattctgca
     3961 agctgatgct ccgggtaaaa aaagtttgtc atacgctgta gtgtatattc atttccaata
     4021 aaatgaacga tgtcccctgc cgtaaattta gcataggggc caggtgaaac aagcagttct
     4081 tcattatgag aaattgcgat aactgtggcg ccagtcgcct gccagatatt caactcgcta
     4141 attgaacggt ccagatgcat tgctttttct gataaagata attcaaacgg aagtaaagga
     4201 ttcatcttat gtattcgctt cgtttgattg accagctgat ccaataattg agaaaaatga
     4261 ttcagctcat cttgttgctt ctccacactt gccatgattt ctgcttttgt atcttggatg
     4321 gattgtacat cttgatattg caataggaaa tcttttgctt tttccttcga agcaacaaat
     4381 gccccacttc catgccgtac ttccatgatt ccaagatcta ccagcacatt gatagccttg
     4441 cgggtagtct caggagaagc attgtacgta ctggcaagtg ttgagcgggc atggatcttc
     4501 tcacccactt tatatcggtt ttcaacgata cgttcggcta aatcaacagc aatctgctgg
     4561 tagcgcgggc ggacgatttt tttctttcca gacattttct ttcctctttc tttttttact
     4621 ttccctcatc ttactatgct ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca
     4681 acgactgatc atcgcttgat ctgttccttt tttactgggc aagctgtttg acagtcatca
     4741 caattcttac cggattttaa acgagtatag acaattgcgc ctgcacttcc aaagattaac
     4801 acacttaata agaatgtcgc catcctattc ctctccttct aatgacaata tagtataaaa
     4861 ctggttcttg gattttggct gacggaacaa aacataaacg agtcctatca tcagtaaaat
     4921 agcaagatac gtccctaacg ctacctgtcc accctccaat aagtgtccca actgataaat
     4981 cacaaaacta actaaataag ctaaaccgca ttgatagcct atggcgatcc aagtccattt
     5041 cttggcattc atttcccgat ggatagcccc aattgccgca aaacaaggcg cacaaagtaa
     5101 attgaaggct aacaatgaat aagctgctgc gggagtatat gtgtgttgta gcacttgcca
     5161 gatttctctg ccattttcgg atacttccgc atgaccaaac aaaataccaa atgtgccaat
     5221 aatattttct ttagcgacca atcctgtaat cgctgcgact gttccttgcc agtttcccca
     5281 accaagcggc tgaaaaacaa ctgccaggat tcgcccgaaa aaggctaaga tactttggtc
     5341 ttcagaaaca ggttgagcat aaaaattata agaagagctg aaccagatca cgatactaga
     5401 aacaaagata atcgttcctg cttttttaac aaaagacttg ctgcggtcat aggtttgtct
     5461 taggaccgct gaccattgag gaaaatgata tgcgggcaat tccatgataa aaggagctgt
     5521 atctccagca aacaaacgtg tttttttcaa gctgattcca gagaaaataa ttgcagtcat
     5581 accaagaaaa taggcagaag gagctaccca agattgatga ggaaaaaagg cccctgctac
     5641 taaggcaata atcggcaact ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa
     5701 acgacgatct tgctcgtttt caatcgtgcg actggccatg acgcctggca ctccacatcc
     5761 tgtagcaatc agcataggaa tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg
     5821 atccatgaca aaagcgatcc tcgccatata tccgcagtct tccagaaagc tcaagcataa
     5881 aaacaatata atcaattgcg gtagaaatcc taacactgct cccacgcctg cgatgatgcc
     5941 gttcaagatc agctcctgca tccatcccgc tacttgccat tcagctaaca atctgccaac
     6001 atgttctggt aaccatgtac caaacaatgt gtcattgatc caatctgtcc ccattgttcc
     6061 tactgtttga atagccagat aatagatcag ccacatcaca aatgcgaaga tcggcaaggc
     6121 taaccaacga ttcgttgcaa tctgatcgat tttgtcactc atcgataatt ggaaagaatc
     6181 atttgacaca acacagagtg tacatagttg ggtaataaat tcataacgtt cattgaccag
     6241 aatcgtctca ctatcgtcgt ccaatagttt ctctgttaac tggatcagtt gctcgatctc
     6301 tttttgctga gaaactgcta tctcgtaatc ctctcttacc ttctgatctc gttcaaaata
     6361 tttcattgca taccagcgtt tcttgccgct ttgttctcct agtacatctt caatttcatg
     6421 taaagctgct tctaaacgtg gatcatatgt acagggaaca ggaatagaag gccgcttttt
     6481 tgcagactcc tgaatctttt cgattagttt ttcgattcct tgctttttca atacgctgat
     6541 tggtaaaact ggtacaccaa gaccatagga aagcttttct agattgattt tttttccttc
     6601 ttttttgatg acatccatca tattcagacc aatgatcata ggaatgtcta aatccaataa
     6661 ctgcgtagtt aaataaagat ttcgttctaa attggtagca tcaataatgt tgatgatcag
     6721 ttcaggctgt tcctgaacca aataatctct tgcaatcact tcttctggtg tataaggaga
     6781 caaagaatag atgccgggta ggtcctgaat atgaatgttc ggattttttt tcaacgatcc
     6841 ttcttttttt tctactgtta cgcccggcca attccctaca tattgattcg aaccagtcaa
     6901 gagattgaac aagctagttt tcccgctgtt tggatttccg actaacgcga tattcattct
     6961 ttttcctccc tttgtatcaa gattagttcg gcctctgact ttcgtaacgt cagttcatac
     7021 cctcgcactg tcagttcgat tggatctccc agaggggcta gttttcggat aactattttt
     7081 acatttcgcg tcaatcccat atccattaac cgtcgcttca ctgcgccttg tccgtggatt
     7141 gctacgacga tcccagtctc tcctgctttt aattgatcaa gagaagtcca gctttcagag
     7201 gaagcagtct ttttattgac aaagatttga cctagtattt cccgacttac tgccactcga
     7261 tgacttttta ccagtaaaat ggcattgttg cctgataaat tgacgacgac gattttactt
     7321 ccggcaacga tcccgatatt ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt
     7381 tcgacttgat aaacgaaatt ctgctttgcc tgcgctaagg tctgcatatt gattcctctc
     7441 ttgtgaaata aatcatgtat taaaatcatt cattatatgt tctaattttc ttacgtgtta
     7501 cgcaaataaa tgagaatcat tctcacatag aatactctcg gtaattgaga atgtcaatta
     7561 tacttgtgat attatgagcg aatctttagt attgatagat aaaaatgaga aaaaaatatt
     7621 tcttcatagt tttccacaat tttttctcta ttaaatttgc tattttccca cacgatactt
     7681 gttttcagct cttttttcaa aaacacaaaa cttagtattc cacagtttcc acaacacaat
     7741 atgtagtgtt tgttgtttga cttttttact aagagtttta tactatctat tatagataat
     7801 aagaatgtgt tagaggaggc actcaccatg aacgtaaaaa tcttttctaa aaataattgt
     7861 atgcaatgta agatggtcaa acggttttta acagaaaatc aaattgcttt tgaagaagtc
     7921 aatatcgatg aacagcctga agcagtagac tggttgaaag cacaaggatt ccaaagtgtt
     7981 cctgtcatta cttctgatgc agaaacagtt atcggattcc gtcctgatca gttgcgtaaa
     8041 cttgccagct aacatgacta aaagaatcaa aacgtgatac atggatttga agggcttgag
     8101 acagcaccag tttttgtcgc aaacttccaa atcctttttt aaagcttaaa aacaatgcaa
     8161 tgatttgcaa tcaagcatca tcaaatacga aatgaaggtt gggcgtacgt atgagtctta
     8221 aaaatttaca agatgtcagt tatttcaagc tgaataatga aatcaatcgg ccagtcaatg
     8281 gtcaaatccc tttgaataag gataaagaag ctttagttgc cttttttgag gaaaatgtca
     8341 aaccaaatac gatgactttt ccatctatta tggaaaaaat caattacttg atcgatcaag
     8401 aatatataga agcagaattc attcaattgt attcaccatc atttattgaa aagctatatc
     8461 aatttttgga cgagcaacat ttttctttca aatcttttat ggctgcttac aaattttatt
     8521 ctcaatatgc gttgaaaacc aacgacggtt ccgcttatct ggaaacttat gaagatcgag
     8581 tagccttcaa tgcgctttac tttgcgaacg gggatgaaga attagcactt gctcttgcag
     8641 acgaaatgat ccatcaacga tatcagccag ctacaccttc tttcttgaat gctggacgta
     8701 aacgtcgggg cgaattggtg tcttgtttct tgactcaagt cactgacgat atgaactcta
     8761 tcgggcgctc aatcaactct gctttgcaat tgtcccgtat tggtggcggc gtagggatta
     8821 ctttatcaaa tcttcgtgaa gccggcgctc ctatcaaagg atatgagggt gctgcaagtg
     8881 gtgtcgttcc agtcatgaaa ttattcgaag atagtttcag ctattctaat caattaggtc
     8941 agcgtcaagg agctggcgtg gtttatttga acgttttcca tccagacatt attgctttcc
     9001 tttcagcaaa aaaagaaaat gcagatgaaa agattcgcgt aaaaactctt tctcttggcg
     9061 tgatcgtacc tgataaattt tatgaattga cacgtaaaaa cgaagatatg tatttattta
     9121 gtccatatag tgtcgaaaaa gaatatggtg tcccattctc ttatgtaaat attacagctg
     9181 agtatgatca tctagttgca aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc
     9241 ttgaaaatga aatctcaaaa ttacaacaag aatctggcta tccttacgta atcaatattg
     9301 atacagcgaa ccgcgagaat ccaatcgatg gcaagatcat catgagtaat ttatgttcag
     9361 agattttaca agttcaaacg ccttctgtga ttaacggcaa acaggaatat gaagtattag
     9421 gaacagatat ctcatgtaac ttaggctcga caaatatcgt gaatttgatg caaagcccta
     9481 actttggtca atctgtgcgt acgatgactc gtgcattgac atttattaca gatgcatctg
     9541 atatcgatgt cgtaccaccg atccaaaacg gaaataagct gaatcatacg attggattag
     9601 gtgccatggg cttacacact tatcttgcaa aagaacaaat ggaatatggc tcagaggatt
     9661 cattagactt tacaaatatt tacttcatgc tgttgaatta ttggacactt gttgaaagca
     9721 acaatatcgc tcgtgaaaga aaacaagttt tccataattt tgaaaaatca gcttatgctg
     9781 acggcactta tttcgaaaaa tacgtaactg gagaattcca gccaaaatca gaaaaagtca
     9841 aagcattgtt tgaaggcatt tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag
     9901 cagtaatgaa agatggactg tatcatcaaa atcgcttggc agtagcacca aatggctcaa
     9961 tttcatatat caacgatacg agtgcaagta ttcatcctat cactcgcatg atcgaagaac
    10021 gccaagagaa gaaaatcggt aaaatttatt acccagcgcc ttatctatcc aatgagacga
    10081 ttccgtatta cacttctgct tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa
    10141 cggcacaaca acacgtcgat caaggcatga gtttgacttt attcatgcgt tctgaaatcc
    10201 cagaaggcat ttatgaatgg aagacaacaa cgaagcaaac gacacgtgat ttgaatattt
    10261 taagacatta cgctttccac aaaggaatca aatccattta ttacgtacgt acctttactg
    10321 atgatgcaga agaaatcgga agcaatcaat gtgaaagctg cgtgatctaa tatcatagat
    10381 agattcgctt attcatattt taattaaagg agcattctca tgtcagaaac atattatgaa
    10441 gcaataaact ggaatgaaat tgaagatatc attgataaat ccacttggga gaagctgaca
    10501 gagcaatttt ggctagatac acgtatccca ctttcaaatg acctagatga ttggcgtaca
    10561 ttgtctcaat tggaaaaaga tacagtagga catgtatttg gcggtttgac actgctagat
    10621 actgtccaat ctgaaagtgg catggatcag ttgcgtaaag atgtccgcac tccccatgaa
    10681 gaagccgttt tgaacaatat tcaattcatg gaatccgttc atgccaaaag ttattcttct
    10741 atttttagta cactgaatac gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg
    10801 aatccttatc tgcaaaaaaa agctgagcgt atcaatgaaa tctacaaaca cggtacaccg
    10861 ctagaaaaga aaattgccag cgtcttttta gaaacattct tgttctattc aggtttttat
    10921 actcctcttt attatttagg aaacaacaaa ttggctaacg tagcagagat catcaaacta
    10981 atcatccgag atgaatctgt acatggaacc tacatcggtt acaaattcca attaggattc
    11041 aacgagttac ctgaagcaga acaagaaacg ttaaaagact ggatgtacaa tcttctttat
    11101 gagctttacg aaaacgaaga acgttataca gaagaattat acgatccaat cggctggaca
    11161 gaagaagtca agacattcct acgctataat gccaacaaag cattgatgaa cctagggatg
    11221 gatccactct ttccagatac tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca
    11281 acaggcacaa gcaaccatga cttcttctca caagtcggaa acggctattt gttaggtcat
    11341 gtagaagcga tgaaagacga cgattatttg attggattgg aataaggatt tattttaatt
    11401 caaaaatttt tcgaaaaata aaaagagttt agcgtacaga tattttaaat gtacactgaa
    11461 ctctttttta tgcttattca cttgtttctt ccagcacttt tggactgtct aacttttccg
    11521 aaacatgttc cattcttctc aataaataga ttttccggat atttaatatt atcgtcttaa
    11581 tcactgcata gacaggtatg caaatcagca tgcctaatat tcctgcgaca cttcctgctc
    11641 caatcaatac caagatgatc gtcaaaggat ggatattcat tgatttccca aataacaacg
    11701 gcttgatcaa atttccatct aattgttgga tcactaaaat cgacaacgcc atatacaaag
    11761 cttgaaatgg agagataaac aaaccgacaa tgattgctgg taccgctcca ataaaagggc
    11821 cgacatacgg gataatattc gtgattccgc aaatgaccgc aagcaacaag ctataaggct
    11881 gatcgaatat tctcattgcc aaaaagctca ttacaccaat aatcaaagca tctaagatcg
    11941 tactgctgat ataggcagat aatgtttcgt ttaattcttt taccgtttgg cgtaactctt
    12001 tacgaatcgc tttcggaaag aagtgagaca acgcatcaat aaatttgtgc ccatccttga
    12061 acataaatag caaaataaat ggaacggtaa ataacaaaat aaaaaattgc atcataaatg
    12121 aaatgatttt tgagacactt gcagataaac tgactaatat gatattgaac aaattactaa
    12181 gagataaatt tgctgatgct aattgttctt ccaaattgaa atttttaaac tcttcccgct
    12241 gcgccaattc atttaaccat ttgcttgttt catctgcata cattggcaag ctctgggtca
    12301 attgaattgt ctgttccatt aactgaggca ctacattcat gacagttaaa acaactaaaa
    12361 tgattacgac caaaaacgtc aataaaaaac cgaacactct cgggatccga tgcttttcga
    12421 gaaaaacaac gattggatcg aacatataat agataaaacc agcaacaagc aatggtaaaa
    12481 aaattgccga aagcattttc aatattggac taaaaatatt cggcatttgt aaaataaagt
    12541 acacgccgat gatcgtgagt actagctcca ctgtccagaa aaataatttt gattgtttaa
    12601 aacgtgattg catacatctc ctccaaggtc ctttgatact tctattctat catttaacaa
    12661 agagttacgc gctgagattt tcatttgctt aaaaaaaaca aaaaaaataa acatatttta
    12721 atgagtagac gagtacttaa acaaaaacac ggtttggagt agtgacaaaa attatactgc
    12781 agtccctttt tccaaataaa cggtgttcaa aagcttgcat cttcgtaata agcccagcca
    12841 aacgaaaaat a
//
LOCUS       DACDLI010000071        12639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDLI010000071 DACDLI010000000
VERSION     DACDLI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.071"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(81..299)
                     /locus_tag="IV353_002153"
     CDS             complement(81..299)
                     /locus_tag="IV353_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9010358.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(296..541)
                     /locus_tag="IV353_002154"
     CDS             complement(296..541)
                     /locus_tag="IV353_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9010359.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(657..1385)
                     /locus_tag="IV353_002155"
     CDS             complement(657..1385)
                     /locus_tag="IV353_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9010360.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(1544..2587)
                     /locus_tag="IV353_002156"
     CDS             complement(1544..2587)
                     /locus_tag="IV353_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9010361.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(2577..3068)
                     /gene="coaD"
                     /locus_tag="IV353_002157"
     CDS             complement(2577..3068)
                     /gene="coaD"
                     /locus_tag="IV353_002157"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9010362.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(3065..3625)
                     /gene="rsmD"
                     /locus_tag="IV353_002158"
     CDS             complement(3065..3625)
                     /gene="rsmD"
                     /locus_tag="IV353_002158"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9010363.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(3744..4082)
                     /locus_tag="IV353_002159"
     CDS             complement(3744..4082)
                     /locus_tag="IV353_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9010364.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(4066..4503)
                     /locus_tag="IV353_002160"
     CDS             complement(4066..4503)
                     /locus_tag="IV353_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9010365.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(4493..5617)
                     /locus_tag="IV353_002161"
     CDS             complement(4493..5617)
                     /locus_tag="IV353_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010366.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(5638..9066)
                     /locus_tag="IV353_002162"
     CDS             complement(5638..9066)
                     /locus_tag="IV353_002162"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9010367.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(9092..10255)
                     /locus_tag="IV353_002163"
     CDS             complement(9092..10255)
                     /locus_tag="IV353_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9010368.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(10258..10560)
                     /locus_tag="IV353_002164"
     CDS             complement(10258..10560)
                     /locus_tag="IV353_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9010369.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(10674..12446)
                     /gene="recQ"
                     /locus_tag="IV353_002165"
     CDS             complement(10674..12446)
                     /gene="recQ"
                     /locus_tag="IV353_002165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9010370.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         3445 a         2746 c         2151 g         4297 t
ORIGIN      
        1 aaatattctt taaatataac gaatcatcct gtctgacaaa agatcctacc cttcactgcc
       61 gaaatgaagt ccgtcatcca ttagcccatt ataaaaattc ttcgaatcca tttgttcaag
      121 aaattccgta taaaaatcga tacttcacaa tcatggcgtt ttgctacttc tgtgagaaca
      181 gagatatatt ctttgatcaa cttgttattc cgatattttt gttttgtctc atcgactgct
      241 ggtggcgtga taaagaaagc atgatcagaa ctatagtctt ttttcagctg aactatcatc
      301 cactgcagat tttttggtac gtttctttag aaagttgttt atgctctgct agatcattcg
      361 tcccaatgag aataaatact ttatctgctg tatagctaag tacatctttg gcaaagcgtt
      421 ctcgtagagc ttgactgtta ttgccgctga cagccgtatt cataatatga atcccagata
      481 ttttattttg gagatagaca ttcagcattg gtttttttaa tgtttctttg cgaacaatca
      541 agctatttcc tatccaaaaa tactacctcc tcgtcctgcc aagctcttag aattggatta
      601 ttcttagtat ccattagcgt ccatttattg ctcgtgccag tcgattttga atcattctat
      661 ttaattgtaa cgaagttttt tagtttttca actgtctttt caccgatacc agaaatctcc
      721 tgcaggtctt ccactttctg gaaggatccg ttttcttctc taaatcgaat gatttcttgc
      781 gctttgactg atccgatccc aggaatctgc tgaagttctg atggagtggc tgtattgata
      841 tttattttgg gaggctcgga atctgctgta ggctgttttc cttccgagga tgccaactga
      901 tcaagatgtt ctggcagctc ttcacctaca gcagggatat ataggacttg ctgatcttct
      961 atcttcgcag aaaagttgat tcgatcgcta tccgcttctt ctaataatcc acctgcacgc
     1021 tttagtacat catagacccg ttcttctgaa gaaaaggaat agataccagg ctccttcaca
     1081 cttcctttga tatcggtgta catgaccttt ggacgagcat cctcttctac atcgtttcct
     1141 tcagctgatg gatcttctcc actggaacta aacgaagaaa gtatttcttt tgcagcagtc
     1201 tcttctgttt ctctataaaa tagcaaattt gataataaaa aaccacttgc gatacaaaag
     1261 ataagaacta tcatagacat ccaaatgatc aatttgggtg aagctagtat tttcttgctg
     1321 attttttcta tcattgttct tttttcaaag aatgaccagt aaaaatcatt cattactcct
     1381 ttcatttaaa attgaagaaa cagtgagctc tggcaaaatc gtcccttcaa catctaatta
     1441 cgtcaatttt ttcttttgtt tttattttta tcgtgaagat ttttgacgaa aaagaggggt
     1501 tgtgacagaa ctcgtgtcac aacccctctt taattattta gtttcatttc attttctcta
     1561 gatagtcaag cgcttcttgt actgttttta ctggtacgat tttcattgag gaattcaatt
     1621 ttttagcggc agcttttgct tcttcgtagt tggtttttat ttcaggatat tctttcttaa
     1681 catcgtctga aatcgtatca tctggtgcaa agaaaatctc tacgccagct ttatccgcac
     1741 ttgccacttt tttatcgatt cctccgattc ggccaacttc tccttcactg ttgatcgttc
     1801 cagttcctgc aatttgctta ccatgtcgca agtttttacc agtgacttgt tcatagattt
     1861 ctaaagtgaa catcaatccc gctgatggcc caccgataga gccagaatta atacgaacat
     1921 ctgtatctga ttcgatctcg gtatggtcag taagcgtgat acctattcct gcctttttat
     1981 ctgttggtaa ctcgatcaga tcaccagaag ctgttttttc ttcaccatta tgcaaataac
     2041 tgatttcgat tgtttgtcca actttttggc tttttacata atccataaat gcttctgtac
     2101 tttgaaagct tttcccgtct acttttgtta ctgtatctcc aactgaaagc ttgccaaaga
     2161 aattcgatgt tttctctaca ctcatgacat atacgccttt gaattccatt ttataaggct
     2221 tgtctgcaag ttttaacgct tgttcgattg ctgcattttg ggaagaatcc atataatact
     2281 gttgcatttg attatattct tcatttgtcg ccccaccagt caattcttct tcactgatca
     2341 attcttcaaa aggggtcatt ttagctttta aagctaagaa acctgtagct tgcctgatac
     2401 caacagaggt aagagagaaa gacccagaac ctttatcttc cttatcattg accgtaatta
     2461 gttctttcag atcgatcgtg gctcccggtg attctacata atacggcaag ggaactacta
     2521 caaatccaat gatcaacatt ccaaatatga taatcagtac tgttttcaac cattgtttag
     2581 ctttcatttg attcacgctt tccagctaaa gcttcgttga tcactggcgg taagtagctg
     2641 ctcacatctc cgccaaagtg cagtacttct tttaaaatac tggaactcac gtgactatat
     2701 tctggttttg ctaataagaa gacagtttcc aattcatcat atagatgatg attcatctga
     2761 gcaatctctc gttcatattc aaagtctttg atacttcgta tgcctctgat caacgcatct
     2821 gcaccaatct cttttgctgt ttctaccgtc aattgatttt cttgatgcat gacttttaca
     2881 ttaggaagat gagaaacagc ttttgtgatc aatgtcattc tttcttctgg gggaaacaaa
     2941 cttttctttg aagtattgat aaaaacacca attactacct catcgaataa tttagccgca
     3001 cgttccaccg tatctaagtg accttttgta aaaggatcaa aacttcctgg gaataatgca
     3061 cgtttcattg atttccctct tttctataga tagttacggc cgttatgcca taaatctgtt
     3121 ttcttatttg atggaatcca gccacagttt ctggcaattc gactgtttta tccgtttcgc
     3181 atacgataac tgcggtttta gaaaaaagtc ccagttcttc cattttatgg atttgatcga
     3241 ttattttttg ttttgcatat ggcggatcaa gaaacaagta gtcgaattgt tgtttctccg
     3301 ctgctagccg ttcgattgct ttgtccgcat caagttttat tgtggtaaat ttctctggtt
     3361 ctttcgtaat ttcgatgttt tctttgatga tttttagcgc ttggtaattt ttgtctacgc
     3421 ataccgcatg actgcagcct ctagatactg cttcgatagc taaaccaccg cttcctgcaa
     3481 acagatccaa tactgtctct ccatcaaaat atggtccaat catattgaat atggattctt
     3541 ttactttatc tgtagttggt ctggtgttcg cccctgctaa actttttaaa cggcgcccgc
     3601 cataatttcc tgatattatc cgcatctact cactccttag aaatggtact ggtactagta
     3661 taccaaaaaa atacttggac aatgatcaaa aatcgatcgt tatccaagtt attttttatt
     3721 taaaaactgc tcataagaga tttttaaaga tgttcagcta atcggatttc tgtatttagc
     3781 tcttcaattt cgaaaccttc gtcttttaat actgttttgt ttcctatttt ttcggcaaaa
     3841 ttcatttcga tatccggtcg gtaagaacgt tctacgtttc gaacaaaatg taatttacgg
     3901 attcgttctt ctgtttcttc caatgtctct tcatttaagt aaagcacgac atatttcatt
     3961 cttcttgaga cataatgcaa caagccgaac tttttcagat tctttaattg ttttaagctg
     4021 tagacccata cgatcagccc gcgtcgtttt gttatttcaa attcactatt gtctaattgc
     4081 atgacaatta cctccgcatc caaagtgttt tttcgtttca aaaaacgggc tacctgcatc
     4141 tactttgatt tcattagaaa cttcttcggc aagtacttgg acaatttgat ccaacagact
     4201 ttgcagctgt gtttcactat atcggaaagc tgcaacatgc tcatccatat caagctgtct
     4261 ttttgctttt cgaacagctc gttttttttc acgataatca ggtgcatact caccatatgc
     4321 cgctacattt tcaaatgctt ctttcttaga caagaacgct tctttcaact ggcaaacttt
     4381 tgcagattct tccattgact gtttatgttc aagataagaa gccatcgttt tactcttttt
     4441 tatcatttca gcgacttgga ggcattgatc ctccagttga aacagctctt cgttaacgat
     4501 cactgctatc actactttct ccgttttctt cttgcaataa gactaacata ttttctttag
     4561 aaaaagcaat gcctacagaa tccgttcccg ggtccatgaa acggtcatgg ttgtaaggat
     4621 cgctgtacca atataaaaac gaaaaagtag cgtcaatcgc cggatgaata aatagcccac
     4681 tagtattttc taatttttgt ttactgatta acttctccaa ttcatcattc gtcagtgaga
     4741 atttcactgt cgtttgagca ctgcctagcg atttttgcca atcagccata cgttcctctg
     4801 acaatatttc ctcaggcctt gtcaaaaaat catgtagtag cagctgactg tggttttgaa
     4861 aggcaagtgt ttgattgtat atgggaagtt cgtcttctct tctaagcata tttaataaag
     4921 tcgtactttt tcgttcttta acgcgattga ttgattccca atcagccgat ttatctgctt
     4981 gataatgtgg aaggccttca ccaaacactt gatatggcaa tagtttcaaa agactatctt
     5041 tatctaaata cacaactgaa aataggccgt ttgttgtttg atcaaaaaat aaaatagcaa
     5101 aagtgtcgtt atcaaaagca attagcggac gataattcat atcctcttcc attagttcga
     5161 ttccaacttc tgtatcatgg tactcaaaag tgaaattagt atagatagta gtgattttcg
     5221 taagatcttg catcgtcatc cctaaataaa acggcgcgat atcagcaggc cctgtttgca
     5281 gtactttaac agttgtgact ctgctgtctt ggatgttcgc ttcaaatgtg agatgactag
     5341 ctttattttc ataatatctt gtttcaaaac caaatccgct agttaatcga tcataggctt
     5401 ggccaaaatc cttttccagc tgatcgatcg gttttccgat ataagtagaa aaaccagttg
     5461 ctttaagctc ctggtatttc cacgttgtca tttggtttga tatttgttga tcagagtttt
     5521 ccttcacttc aagttttggc ggaaacaaga ctggttccat gtagaatgcc acaataacta
     5581 atagcagaat cagccaaaac aaacccagtc ttttcatgtt tccccacctt tcgcttatta
     5641 tttctcagtc acttcaatca aaaggtcacc agacgaaata gtgtctcctg aagacacata
     5701 tacatgtgcg acttctccgt caaaacgtgc ttcgatggcc gtttccattt tcatcgcttc
     5761 agtaatcatc aacgtatcac cttttttaac gttatcgcct tttttcacta atacatccaa
     5821 aacagaacca gacatcgttg ctccaatttg ttctcggttt gtcggttcag ctttcaatcg
     5881 ggtttggacc gtactgatga tcgaattatc tttgatagtg atttcacgtc tttgtccatt
     5941 caaattgaag aacagtacgc gattcccttc gatatcgggt tctccgattt catctaaacg
     6001 aatgataagc gtttttccgc gttcgatttg tacattgatt gtttcaccca aacgtatacc
     6061 ttggaaaaat gttggtgtgt ccagtaatgt aacatcgcca aattgattat atgctttttg
     6121 atagtccaaa aagacttgcg gatacatcaa atagcttaat acttcgtctt gttttggttc
     6181 atagccaatt ttttctgcca attctttttt tacttcgttg aagtcgactg attcagccaa
     6241 aagacctgga cgttcattca atgcaggtct gcctttaaga ataatctttt gcaatttttt
     6301 aggaaatccg ccaacgggtt gccctagttc tccttggaag aaagtcacaa cagattcagg
     6361 gaaacttagt gtttcgcctt tttcataaat atcttcttct gtaagatcat tttggaccat
     6421 aaatagtgcc atatctccaa ctactttaga tgatggagtt actttaacga tatcgccaaa
     6481 catcaagttg acagtatgat acatttgttt gatttcatcc cagcgatgac ctaatccgac
     6541 agcttttgcc tgttgctgta gattcgaata ctgtcctcct ggcatttcat gcatatatac
     6601 ttctgtttca ggagcattca agccgttttc aaacggtttg taatacatgc gcacatcttc
     6661 ccaataatgg tttaatttct gtgcattttc aatcgtgatt tctggcagcc gtggaccatt
     6721 gaccagtgca taatacaagc tgctcatgct aggctgacta gtattcccac tcattgcact
     6781 cattgccacg tcaacgatgt ctactcctgc ttttgaagca gcagaataag tgatgattcc
     6841 attcccgctg gtatcatgag tatgcagatg gattggcaga tctgtcgttt ctttcaattc
     6901 actaatcaaa cgataagctg cctgcggttt aagcagccca gccatatctt tgattgcaat
     6961 gatatgcgca cccagttgtt ctagttcctt tgccatgtct ttataatact gaacattgta
     7021 ttttgcccga cttggatcgt tgatatcgcc agtatagcaa atagctgcct ctgcgatttt
     7081 accagtatcc cgtacagctt ggatactttt ttccatttgt ggagtccagt tcaaactatc
     7141 aaagattcga aatacatcga ttccttgcgc tgctgcttct ttgacaaatt ccaccaatac
     7201 attgtcagga taattagaat acccaacggc atttgaacct ctgaaaagca tttgcagcaa
     7261 cgtattaggc attaaagaac gaatttttct caaccgttgc caaggatctt cattcaaaaa
     7321 gcggtaagcc acatcaaatg ttgctccccc ccacatctca cttgaaaaga gttcaggaag
     7381 accttctcca gtcaaacgtg caatctgctt gaaatccttt gtccgaacac gagttgctag
     7441 cagactttga tgggcgtctc taaatgtcgt atccgtcaac aacaaatttt cctgtccttt
     7501 gatccattcg accaaagcat cggcaccctt cgcatctaat acattcttag ccgttacgta
     7561 atcagttctt gtgattagat ctttaggcat acgtggttct tcataaaatg gtttttcgcc
     7621 actttcgata cctggaaaac cattcacagt aatttcaccg atatatttca tcgttttgtt
     7681 tccgcggtct cgtagtcgag gaaattcaaa taatgttgct gtactatcaa tgaaggttgt
     7741 ttttgcattt cctgattgga attcaggatg tgtgatgaca ttgagcataa atggaatgtt
     7801 tgttttcact ccacggattc taaattcttt caagcaacgt tccattttct gaatggctgt
     7861 ttcaaaggta gcaccatgcg tacatacttt tactagcaat gaatcaaaat acggtgtcac
     7921 tacatagccc gcgtaagcat ttccgacatc cagacgtacg ccaaaaccac caggtgaacg
     7981 atacgtatcg attttccctg tgtctggtaa aaagttattc aacgggtctt ctgtagtgat
     8041 tcggcattga atggctgaac catttaattt aattccttct tgctgaggca atccgatttc
     8101 tttatgcaga tccaaacctt gagcaatcaa cagttgcgtt gttacgatat caatatctgt
     8161 aatcatttct gtgatcgtat gttccacttg gacgcgaggg ttgacttcga taaagtaaaa
     8221 atcatctcct tcaacaagaa attctactgt acctgcgttg acatacccga catatttcat
     8281 caattggaca gctgcctggc agattttttg acgttgctgt tcattcatag agacacaagg
     8341 cgctacttct actacttttt ggtgacggcg ctgaacagaa caatctcttt caaaaagatg
     8401 gatgacgttt ccatgcgtat ccccgagaat ctgtacttcg atatgcttag gattagcgat
     8461 gtatttttca acatacactt catcactacc aaaggctgct tttgcttcgc ttttcgcacg
     8521 ttcgtagccc tctcttgcac tcttttcatc gtgagctaca cgcatgccgc gtccgccgcc
     8581 gcctagagct gccttgatca taatcggata accgtattgt ttcgcaaact ccaaggcatc
     8641 gtctatagaa gcgattggtc catctgtgcc tggaatcgaa gcaatccccg cttcgatagc
     8701 tgcagctttt gctttgattt tatctccaaa aatatctaaa tgatgcagtt tgggtccaac
     8761 aaaaatgatg ccttcttctt cgcaccgttg cgcaaaattc aagttttccg acaaaagacc
     8821 gtaacccgga tgaatagcgt cagctccgca ttctttcgcg atagaaatga tcccttcaat
     8881 atctagatac gcatcaatcg gtttttttcc ttgtcctact aaatatgctt catccgcttt
     8941 gaaacggtgt acggaatatt catcttcttc tgcatagatt ccaacagttt taattcctaa
     9001 ttctgtacaa gcacgaaata cacgtacagc gatttcaccc ctgttagcaa ctaaaacttt
     9061 tttcatcgaa ccactcctac atcttttttt attataaacc taagctcttg cgtttttcat
     9121 ccgcactgat gttgaggaca aagccgacac aaatcgaaag catcagcaaa ctggagcctc
     9181 cttgacttaa aaatgggaac gtgatccctg tcaaaggaat aacgccagta attcctccaa
     9241 gattgacaaa tacttggatc aaaaacattg cgccgatccc aatgcacaaa agcgaattga
     9301 acggatcttt tgaacggata cctacaagga caatgcgcac aatcataaag aatagcaacg
     9361 ctaatatcaa aatccccata atcactccca gttcttccac tacgattgca taaataaaat
     9421 cggtttgtgc ttcttgtaaa aatccttttt tctggatact gttacctaat cccctgccaa
     9481 atagtcctcc gttgaacatg gcatagtagc cgttgactag ttgatgccct ttatttaatt
     9541 cgtctgagaa aggattttgg taaatagcaa atcgattata gatatactgt aaacgtccgg
     9601 gtaagatctt tccgtttgtg atattgatca gccagatcgt aaaagtactt agacaaaagc
     9661 ctcctactcc tacaatcaaa gtatacacat aattgactcc gctggctaaa agcagaacca
     9721 aaataatcaa aaagataact gccgcattac caaaatcggg aagaatagcg actaatgcag
     9781 taagtccgat aaccattgca agtggacgtt tcacagtccc aagaaaatct ttttgtacac
     9841 tgttttgtct tttagataaa gtcagagaca ggtaccagat agaaatgatt tttaggtatt
     9901 cggcaggttg gatcgagaat cctgcaattt gcagccaacc tttcgcccca ttgatctcct
     9961 taccaaaaaa cacaacaatc agcagcaaaa tcgtcaaaac tgcgatggct gccatgatca
    10021 aacgctggtt tttcaaaaca tctgttttca ttttataaag tagtgcgata gcaatcaagc
    10081 taagcaccca aaaaatactc tggttaatga ctgatgcgga cggattttgt ccgttttcta
    10141 ataatagata cgacgtagaa ctatatacca tgatcaatcc caatacacat aaaattaaat
    10201 agggaatcaa gatgctatag tctaatagat gccttttttt cactttctta ggcaagtcta
    10261 ttcctccttg ccaacttctt ttttatcata aatttcttgg taaacctcac tatataactg
    10321 attcaattct cgttctagct cacttaacat cagctggccc tcgttctttc cgattactcc
    10381 tatacggaca gcaaaaccga cttgccggga aaagccgtac atctgtgtat ctaccacttc
    10441 ttcgaaggct ttgcattgtg agatacagag actgttgttt tgatttcgga ttaatcgttt
    10501 gatccgttct gcttcatcat ttaatacttg gatcgcaagt tttttcgaca aagaatccat
    10561 ctgtgcttac ctcccattct aattccttaa ttataacata tttgattttc ataaggagca
    10621 aaaaaaacag atttaaccag ttgtttttat tcatttttac gtattcgttc tttctactta
    10681 tccagaacgt tagagtccgc attttttatc cgagataaaa aggcttcgcc atatttatca
    10741 agcttgcttt ggccaactcc ttttatctga agcatctcca atctgcttga tggacgtatt
    10801 cggctcattt ctttcaatgt ggaatctgaa aatacgacat acgggggaac gcctgcttct
    10861 gatgctagat ccgttcgcag ttcccgcagc acttcaaata gtgtatcggt ttcttcatcc
    10921 gatagctgct ggactttctt gggttccttt cgataaacag attcttgtcc tttcaatact
    10981 tgtatccctc tttctgttac tttcaaaaca ggatactcac cattgatggt cagaagatag
    11041 ccattcgata tcaaatattc aattaactgc atcgtttctt tttgcgattg gtttttcatc
    11101 agtccatagg tagataaatg tccaaaacct aaagcttgca gcttttgttc tttcgatcct
    11161 gctaagactt tcatcagcat ttgtttacca tagttctcac ccatacgttt taagcaagaa
    11221 agaaccatct gtgcttctgt cgttacttct actatttctc tgtcatctaa acagttacca
    11281 caacggttgc acggactgct tgtctcgcca aaatagttta ggatgtattg ctgaagacag
    11341 gactcaatat acacgtattc tgtcatcgct tttaattttt catattcttt ttgcttttgc
    11401 ccttcttctc tttgggactg ctggataaaa aacttttgga cttggatatc ttgaggagag
    11461 aaaagcagga tggcttcact ggaaagacca tctcttcccg cccgaccagc ttcctgatag
    11521 tatgcttcta gagagccagg aatttgataa tggataacaa aacggacatt gcttttattt
    11581 atacccatac caaatgcatt tgtcgctaca atcagctgca agcgatcgta taaaaaggct
    11641 tcttgcattt ctgttcgctc attctcattt agaccaccat gatatctacc cacggaaaaa
    11701 tcgaattttt taagcaaatg atagagacgg tctacttctt ttctcgttgc tgcataaatg
    11761 ataccagatt gatttttgtt gatctttaaa tattcgatta gatattgttc ttttttttga
    11821 tctttaatta cttgaaatcg tagattttct cgttcaaaac cagtctgaat atggttgttt
    11881 tctggaattt ttaaaagtct ttttatgtca gcagctactt gcacagtcgc tgtagctgtc
    11941 aaagcaacaa ttacaggacg atttggcagt tgatctagct ggttcgccat ctgtaaataa
    12001 cttgggcgga aatcatgccc ccactgagag atacaatgtg cctcgtcaat agcgatcata
    12061 cggacgttcc atcgcttcat tgcttggata aaatccggca tgataaaacg ttcaggagcg
    12121 atataaagta aacggtactg ctgtctatcg atttcttgga agcgtctagc tgtttcgtaa
    12181 ccatctaacg agctattgat aaaagtggcg ggtataccta attgattcgc cgcgtccacc
    12241 tgatccttca tcaaagagat taaaggggac acaaccacag taagcccatc taatagtaaa
    12301 gctggaagct ggtaacagat tgatttccca cttccggtag gcatgattcc taatacattt
    12361 tcctgatcaa tgactttctg gataatttct ttttgaccag gtctaaactc atcatagcca
    12421 aagtattgtt ttaataagtc gttcatcttc ccgctccttt ctcttgccat tttacttcga
    12481 ttttcttcga aggacaagtt gatctccttt cgtgaaacat tatcttatta tgaaacaaaa
    12541 actctctaga aataagtttt ttaaaagtct tttaaacgtc tttcggcgta ttttctgaat
    12601 ttatgtccat tgacaaagat gacttctcca cagaaaaga
//
LOCUS       DACDLI010000072        11919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDLI010000072 DACDLI010000000
VERSION     DACDLI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.072"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            39..269
                     /locus_tag="IV353_002166"
     CDS             39..269
                     /locus_tag="IV353_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010371.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            428..1261
                     /locus_tag="IV353_002167"
     CDS             428..1261
                     /locus_tag="IV353_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP9010372.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            1254..2669
                     /locus_tag="IV353_002168"
     CDS             1254..2669
                     /locus_tag="IV353_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9010373.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            2681..4210
                     /locus_tag="IV353_002169"
     CDS             2681..4210
                     /locus_tag="IV353_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9010374.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            4302..5180
                     /locus_tag="IV353_002170"
     CDS             4302..5180
                     /locus_tag="IV353_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP9010375.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5182..5499
                     /locus_tag="IV353_002171"
     CDS             5182..5499
                     /locus_tag="IV353_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010376.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            5548..6249
                     /locus_tag="IV353_002172"
     CDS             5548..6249
                     /locus_tag="IV353_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP9010377.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6264..7154
                     /locus_tag="IV353_002173"
     CDS             6264..7154
                     /locus_tag="IV353_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP9010378.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7177..7392
                     /locus_tag="IV353_002174"
     CDS             7177..7392
                     /locus_tag="IV353_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010379.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7404..7745
                     /locus_tag="IV353_002175"
     CDS             7404..7745
                     /locus_tag="IV353_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010380.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            7745..8116
                     /locus_tag="IV353_002176"
     CDS             7745..8116
                     /locus_tag="IV353_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010381.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8109..8504
                     /locus_tag="IV353_002177"
     CDS             8109..8504
                     /locus_tag="IV353_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP9010382.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8506..8883
                     /locus_tag="IV353_002178"
     CDS             8506..8883
                     /locus_tag="IV353_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010383.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            8884..9492
                     /locus_tag="IV353_002179"
     CDS             8884..9492
                     /locus_tag="IV353_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9010384.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9492..9833
                     /locus_tag="IV353_002180"
     CDS             9492..9833
                     /locus_tag="IV353_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010385.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10048..>11919
                     /locus_tag="IV353_002181"
     CDS             10048..>11919
                     /locus_tag="IV353_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP9010386.1"
                     /translation="MNNDDLILKMILDESGFTAGMNNAVKKLNSFDETIERKSRNSGS
                     SLGSIWKIFAGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNS
                     EIMKNIGKNGLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFG
                     GSTDQVNEAVIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLS
                     QGKISVEEFQNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIE
                     GLNKALVDSDLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALK
                     FMMPFLVPAATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVL
                     IIGAVVGAIAVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEM
                     ASNAVEGVKDAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKW
                     FSDTWQSIKDSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKN
                     VFEILKNVILAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFT
                     "
BASE COUNT         4354 a         1636 c         2437 g         3492 t
ORIGIN      
        1 ttgagtggtc tttttcttta cataaaggag gctacataat gagaaactac tggtatgtat
       61 cgctaactaa tgaatatcct cgaaccattg atgattgttc agtgcgtgtt gtgcgttctg
      121 tacaaatcaa agggaagtac tctattgtcg aaatggggag agaagcagaa ccatgtgaga
      181 ttgatgcgtg caagctcgtt tattgcggtc atggtttcta tgatgaacca aacattcaaa
      241 ataatattaa caagaatttg agggattaga atgcaagaaa tggcttaaaa atctccaatc
      301 aggacaaatc aagttagaca aagtttccga tttaaagata ttgattgaag ctgatttaat
      361 gctaaaagat attgaaaatt agaaaacaaa actcaaccta aacagaatgt gaggtggtgt
      421 gtattgaatg gcaagacaac gtgatccaag acgtgatgaa gccaaaagaa tttggttaga
      481 atccaacgga gaaaagcagt taaaggaaat tgcatctgaa ttaaatgttt cagattctca
      541 ggttagaaaa tggaaatcgc aagacaaatg gagcgctgaa ttgattagta acgttaccaa
      601 tggcaaaagt aacgttacta atcaaggtgg cgctcctatt ggtaatcaaa atgctaaagg
      661 taacaaagga aatagccgag cttctccgcc agtgggtaat aaaaacgctt tgaaaacagg
      721 tgagtatgaa acaatatttt ttgacacgtt aaccgataat gagaaggaca tctattctag
      781 tttgaatgat gatccttctt ttgctttgtc cgaagaaata cgacttctta aaataagaca
      841 acttcgaatg atgaaaagaa tccaacaagc tgaagctgga ctaaatgatg aagaagtcga
      901 acgattgcag cagctaagaa agattaaaac tccgattgaa aaaaatggta aaaagctaga
      961 aatcaagcgt gaggttatgc aagatgtgca gattagcaga aaaaaacatc gcaaaattga
     1021 tgatattctt tcaattgaag attcattgac tcggattagc aaccagttag ctaaagccat
     1081 caagcaaatg aatgaacttt atatgaatga atacagaact gatttaatta aagctcagac
     1141 tgataagatc caagctgaga caaatgaaat tggcggaaat aattcaggtg aagaaataga
     1201 agaatggaaa caggcagttt taaatgccgc aaacaaacgg gcggtgaaag aaaatgaata
     1261 atgaatttat tccttttgct gatattggtt ctgccattga ttattactat gataaaccag
     1321 tagctttttg ccaagatatt ttgcatttga atcctgatga atggcaagaa aatgttttaa
     1381 atgatttagc tgaattttca aaggtttctg ttcgttctgg tcaaggagtt ggaaaaacag
     1441 cattagaagc aggagcaata ctttggttct taacgtgtcg accctacgct aaagtaatag
     1501 caacagctcc gacaatgaag caactttacg atgtactttg ggcagaggta gctaaatggt
     1561 taaatgatag cttgatcaaa aacttactga agtggacaaa gaccaaaatt tatatggttg
     1621 gtgattcaga gcgttggttt gctacggcta gaacagcgac taaaccagaa agtatgcaag
     1681 gttttcacga ggaccatatg ttgattgtgg tagatgaagc ttctggtgtg tctgatccaa
     1741 ttatggaagc tattcttggt acgctatcag gttttgataa taagctgttg atgtgtggaa
     1801 accccaataa tattgaaggt gtcttttatg attcccataa ttcagaccgt gataaataca
     1861 gagttcataa agtatcaagc tatgatagta aacgtacaaa caaagacaat atagaaatga
     1921 ttcttaaaaa atatggaaaa gaaagtgatg ttgctcgtgt ccgtattttt ggagaatttc
     1981 cgaaaggtgc gttggattca tttatcagtc ttgaaacagt tgaattggct acagaaaaac
     2041 aaattagtga ttctttagtc aataaaacaa aggttgctca tattggtgtc gacgtagctc
     2101 gatatggtga tgattctacg attctctttc ctagaattgc taccagggca ttggagtatg
     2161 agaagtattc aaaacgtagc accatggaaa caacaggata tgtcatcaac atggccaaga
     2221 atctaatgag tcaatatccg agtattgata aagtgatgat taaagtcgat gacactggtg
     2281 tcggaggtgg tgtaaccgac cgcctagaag aacttataga agacaaacat tatccttttg
     2341 aggtgtttgg agtgaataac ggttcaacat cagaagacga tttttacgat aatttaggta
     2401 ctcaactatg gggaaacatc aaggaaatgt tagaagaaaa tatgacagca aatcttaacg
     2461 gagaacagcc tgttattgaa ttgccttctg atagttcgtt aatcaaagaa ttaagtactc
     2521 gcaaattcaa aatgacaagt agaagtcgta tacgtttaga aagtaaagat gatatgaaaa
     2581 agcgaaatat tggtagtccc gatattgctg acgcactggc tttagcgttt tatgagccac
     2641 caagtcacta tcaatttatt caattttagg aggtgagctt ttgattagta cagtattatc
     2701 tttagagaga tataagaagc tacgtgttaa atatgcaaca caaattgaag atggaatgtt
     2761 tgatccgaat ggttttatag aagatatgaa accatttttt gctgatcgcg aaagaaaata
     2821 tctagcttat actagcgaaa agaatgaaat agataataga ccaaagccta acactgatat
     2881 tgtaaaagtt aataataaac ttcatgctgg tatgtattca attgtcgtag accaagcagt
     2941 caatcatttt actggtatac ctatcaaatg ggattacgat gtatcggaac aaaaaagaac
     3001 actcattcaa agattaaaag ataaattctt aaaaaatgat atagaacttc cgacagttcc
     3061 agaagctttt aacaaattaa caagcaatct tgattccatg agattcgcaa tgcttgattc
     3121 tgaaactgca acttttcaag gagcttgtgg agtagctttc cgattgctag agcctgtgga
     3181 agaagatgat ggttggaagt taagagcaag taatattgaa ccttggagag cagaaagata
     3241 tggaaatgcc ggaatctata tcaaagaaaa gtatgactct taccagaaaa aattttttca
     3301 agaaatgaaa gttatgacaa gaaataaaat tctgacatat gcttgctatg gtgatttgaa
     3361 ttttgttaca agcggaacgt tcaaaaaaat tgatgagacc gataaccctt tggggacgat
     3421 taccttggca gaatttaaaa ataatacgaa tcgttattgt gattttgaag tagctgagga
     3481 aattggagat gcaattgatc gtgctttgtc tgatcaacaa aacgaaatcg aacaatttaa
     3541 gcttgcgtac atgcttgtta ctggcacaac aatgagcaaa ggcactgcta aagaaatgat
     3601 gaatcagcta gggattatca acttaaaagc ccctactgca aaagctgaat atgtcacaaa
     3661 aaatttagca aaagatttca atgaatatca tatggatcta ttgaaaaaac agttttacac
     3721 catttgtaaa gcaatcgatt ttaacgatga ggtgtttaaa tcaaacagtt ctggagaagc
     3781 tcgcaagtgg caaatcatta gcctagaagc gaaaacaaat actaaagagc aatattttag
     3841 agaaggattg aaagaatgcg cagaaacaat tgctgctttt cttaagtttc atgacaaagt
     3901 agaaattgag cctgaaaaaa ttatttttac tttctctaga tctttgccaa ctgatttaag
     3961 ttatttagct gaagctttac ctaaacttgc accatacgta tccaaacgaa ctattcagag
     4021 ccaaattcca tttgtaacag atgttgatta tgaaaatgaa atgatggaat tggaaagtgg
     4081 aagtgcttat ccagatagtg aatacaattt tggcggaggt ggcaatagtg acgatagaaa
     4141 cgaagtattg gaccaaacgt cgagaactgg aggatcaagc aaggctcaaa caagaaaatc
     4201 agacacttaa aaaattaact agtgtatttc ctgaagcact gaaagagata caagcaaaac
     4261 tattatcaca agctgactta cacaatatca cttatccaga aatgatggag ttttacagta
     4321 caagtaatca gaaaaaatat cgtgaatatg tggaaaaaaa ttataagtca ttaaaaatgt
     4381 atgatgcaaa atacaaagag tttatcgatg aattttttcc accatttgac tatgcaaaag
     4441 tcaatcgctt attacaaata cgatcagatg tatttaaaat tcttgcagaa tatgcgatgg
     4501 atgcagatgt gaatcaatat ttttctgatc gcttagagga aattcttcaa agaacatatt
     4561 cttctaatgc taatgttttt gttcaacttt taaacgttga tataccaaat tatttaccag
     4621 aaaatgaaat taaacattat ttgaactatc catggtcagg aaagacattt tcaagaagac
     4681 tttggggaaa tatttcatcg cttgaacaga aactctctaa tgctattgta aaaagtgttg
     4741 ctagtggaga aggcgttata cacgcattaa acaccatgag attagactca gaaatttgtg
     4801 acatgtttaa gttagaagaa tcaaagtata acaaagcgat agaaaatctc gttcgaacgg
     4861 agtatgcaaa atttgcacaa gatggtattg aaaaatcata tttagaaaca ggtattgagg
     4921 aatacaacgt attgactgca aaagatgaga gagtttgccg gatttgtgga ggaaaggcaa
     4981 gtaagaatcc ctataaactg aaagatgctg tcataggtga aaatcgagca cctttccata
     5041 gtcgttgtag atgtacggat gttcctaatt taccaaaatt aggaaaggat attgatgaag
     5101 aatatgaccg tttatttggc gatttattag atgagtttgc acatgattct tttggaatta
     5161 atttgaaacg gaggaagtag aatgaaagat ttttttgaag cagtactaac aattaatgta
     5221 aatgctgata ttgcagaagc ctacaaaaca gctattgagt ctgagaacca tcctaatggc
     5281 ttgagagacc attggaatgg caattatgcc tacgtggtta ttggcgatca aactgttaat
     5341 tatcaagata atactccagt tgataagaat accgttaatt taacgattca attattatct
     5401 cattcattac caaatttaaa agaaacagtt gattggtatg aaaagatggg atgtattgtt
     5461 gttagaactg actacaaaga aggaaagtct agtaattagg cttttttatt ttgtccgaaa
     5521 tgacgttaaa ctagcgcaat actgggcttg gttgaatggt ggggcgcaac tattaaaact
     5581 caaagcaatg cggggcgtga aaacgaatcg tggggcgaaa ggagaatgag catgaaacac
     5641 aaatcattaa tgccaatgaa tttgcaatac tttgccgaag gtgacgatca aaagttttct
     5701 tttgatgact tcaaatcttt tgtggaatcc aatgaagaag ctcaaaaatt tattcagtca
     5761 caatctcaaa gtgttgccga caaacaattg gaagcttgga aacaaaataa cttagataaa
     5821 attaaacagg ataccatcaa ggaatatgag gaatctaaga aaaacaagtc acctgagcaa
     5881 attcaactgg aaaaactaca agctgaattt gaagcagaaa aagcgttgcg tgtgacaagt
     5941 gataataaag catttgttgc agaacaaatt gctggattag aactagatgg agagttaaaa
     6001 gagtctattt ctcaatttat gctaaataat cttgttagtt cggatacaga tttcactaag
     6061 aatgctgttg aaggttttac aagtgttttg aatgcaatta atgagaaaca tgcagatgca
     6121 ttaaaagaac tacaaatgaa gtctgcattt ggtggaactc aacaatcgaa taaccaggtt
     6181 cagcagaaca atgaaacatt aacaaatcca gaagaacaat taggacaaat tcttcaacaa
     6241 tttaactagg agagtgaaaa attatgaaaa aaacatcttt aaataattta gagtatttgg
     6301 atatttcacc agcgattaat gctatgcaag taccaaatac acctttttta agctatttac
     6361 ttggtgctgg aaaaacagag ccagcaaact cgacagaaat taaatggcgt gaatatgata
     6421 tcaacaacga tgattcttct gaaaaacttg agggcggaga atatccagat gctgaatcag
     6481 gtcgaacttg gtttaacaac tatactgaaa tttttagaaa atcaacctct gtatctggta
     6541 cattagatgc tattaatgtg aatggagtcg gaaatgaatt aactaatcaa gtagcactac
     6601 gtggtatgga aatgaaaatt gatttgaacc gaaaattgat tactggtgta aaagctgatg
     6661 aaaatggttc taaaggtcgt cgaatgaatg ggattttgaa cttgatcaat tcagcaaata
     6721 aggcagaaac agccactgcg ggtgcagtaa caagaaaaga tatcgatact ttatttaaat
     6781 tgatgtatga aaaaggttat atgggagaaa aactatgctt gatttctcca gatatgcagg
     6841 agttaatgac tgatgagtta gatggaaaat caacaaaaat tgttcagttc ggggaaagag
     6901 taacttttgg attgcaaatt ggaaatatcg tgtctaatta cggtacaggt attgctctac
     6961 tagaaccatc attgccaaaa ggaacaattg ccgcaatcga tactaattat gtgaaactac
     7021 gtccattacg tgaatggaga gcagaagaac ttgcaaaaac aacggattct agacgtatcg
     7081 gtcttgtagg tgaatactct cttgaataca atgcttcaaa ctctggggca attttaaatt
     7141 taaagtctga ataaaaggga gttagtactc ccttttttga taggaggaat tacagtggta
     7201 aaaaaagatg aaactaaaaa agatgaagtc gtgaaatata gagtaggtaa aactaaaaat
     7261 tttgttgggt ttgttcatcc taaaactcgt agatttatca cagcagattc aaataacgaa
     7321 tttatcattt ctatagatga taaagaagca attgcaattt tggaagatgc aattgatgtt
     7381 aatgaaattt aggaagtgat ctgatggatg aatcgctaaa aacggaaatc attgagtcta
     7441 caaaagaaga ttttccagat ttgagtgaag aacgcataac taatttatta gaaataattt
     7501 tgctagaaat tgaatcatac aacacttgta aaaatgatat ttcatgggaa aagttaaaaa
     7561 gcgtgattag tgaagtgttg tataaaataa taaaaaatga atcagaaaaa acagtatctt
     7621 cagttagacg tggcgataca acgattagtt atgcttcaac aacaaatgat gttagtgagt
     7681 tacttctagg ctatggtgat ttgatacgaa gagttattgg ttgtggagga ttggagtttt
     7741 tttaatgaat gaagcagata ttttagaaac tacttatgaa gatagttgta ttattgaaag
     7801 actgacggac attgaagatt ctaatacaaa tattactatt caagattaca aaaaagtata
     7861 tgataatcct atttcttgcg ctctttcaca aggtcaaatc gatggactag cagtcataga
     7921 agatggagag atggtaaatg tttcaactga cacatataaa ttatttgttc atcctaagat
     7981 taaactaaaa aaaggagatc gaataacaat aactcaaaag gccagtggct taattttttc
     8041 tctatttgct actaagcctt tttactatcc tagtcattgt gaagtgaatt tgataggaag
     8101 tgagaaaaat gggtaatctc aaatatgaat caaatgcaga aaagatcatt gagaatttta
     8161 aaaatatgac tgtaattgct caaaaagaag gaatatcctt cgttaacgat tcaatgaata
     8221 aagtcgttag tttagctaaa ccattaactc ctgtaaaatc aggtaattta aggcgaggat
     8281 atcgtgtagt aaaagctaga aaactatcaa gcggtcgaat tgttggagaa gttataaaca
     8341 atgaacatta ttttaaatat gtagaagaag gtcataggac taaaaatggt ggctttgtca
     8401 aagggaaatt catgttaact cgtgcaacga atcttgcaaa tatgtcttat attcctcgaa
     8461 gatttaaaca aatggcaata aaaatcgtta agaaaggaaa gtaacatgaa agatgaaatc
     8521 attgctgcaa tcagtagcaa attaaaagaa atctatcctg atgggacaat atatcttgat
     8581 tcagttatgc agtcaactaa agatttttat ttcgttttat cagtaatgga atctggaact
     8641 gaaaatgtag gaattgatat tcaaaatgtt tctttcttaa ttgatattgc attgattgat
     8701 aataacccta atagaaattt aattaatgaa ttagtctcac gttgtgggac tttttttaat
     8761 acgattacaa tcgatgagca aatattattt ccaaaagcct atttacctga tgaagcagat
     8821 ggtgttcaac atattcgttt tacattagaa tttccacaat atattgaatg gagtgaaaga
     8881 taaatgggag aaaaaagaag taaaaccgga attatttctg tcgaaaaacc tacttggttt
     8941 cctttggaag atgagacagg gaattttccg gtatatggta ctgcgttgcc aatgggaaca
     9001 gcagtaagta tcaaaccaac agctaattat gaaacaacac aagattatgg ggattcagtg
     9061 gttcaagatc aatttacagc ttttggtggt gcagaagttg aactggaagc aaatggatat
     9121 actcataaag ttttatctac aattactgga gggaaaattg ttaaaggtgg tgccttgcgt
     9181 tctggagaag atattgcgca agatggtgcg tttgcatata gacgtaaaaa atcgaatggg
     9241 aaataccgtt atacggtctt ttataaagga cagtttgctc tagattcaga tgaaacatca
     9301 acgattgaag gaagtaaagt aagttttact catccaactt ggaagggttc atttgtagat
     9361 gtaccaggac ttggatatat gtattcagtt gatgaagatg atgaaggcgt ggataaagca
     9421 atgatcggaa attggtttac aaaagtagca attccaattg aagaagcaga actttcagga
     9481 ggtacagaat aatgtcaaaa tatcaaacaa cgattaaatt aacgaaaaaa gacgaagaag
     9541 gtaaatatga gcaagtacaa tttaaatctg ctgaattttt accaggaaca gttgtagaag
     9601 atgctgcagg agttatggaa gaaatgcaaa cagcgactga taagcaatca gttaaaaaag
     9661 ctttaagtcg tgcttattca tttattgcag atactttatt tgagggacaa tttactggtg
     9721 aagattattg caaaggaatt gatgctcgtg agattgcctc attgacagga aaattattga
     9781 agtctgttac tgcaggtttt gatgaaactt atacagagac gaaaaaaaag taagtgaggc
     9841 gctcaagtca ccttcattta agtattcgat tacttaccga gaattagata taaagacgca
     9901 attactcgaa gcaggttgga cgttaccaga aattgaacgt actgacttgg atgagttaat
     9961 gcgtcttttt gcttttagag atgcggtaaa agagcatgaa gatgttgagt actatgataa
    10021 tttcactcaa ttttaggagg tgatactttg aataacgatg acctaattct gaagatgata
    10081 ctagatgaat ctggttttac tgccggtatg aataatgctg tcaaaaagtt gaactctttt
    10141 gatgaaacaa tcgaaagaaa aagtagaaat agtggcagtt cattaggtag tatttggaaa
    10201 atatttgctg gaagttttct agctagcgga gtaactagaa tcgtaggagc tggctttgat
    10261 ctaatcaagg gttccataag tggagcggtt gatcgagtag atacgatgaa taacgctcta
    10321 cgaaatttcc aaaatatggg atttagtaat tcagaaatta tgaagaatat cggaaagaat
    10381 gggcttttat ctcaaggtat tcaaggactt cctactgctt tgaatgatgc gataagtcat
    10441 gttcaacttc ttgcttcttc tacaggtgat ttaactcgtt caactcaaat atttaaagct
    10501 ttaaatgatg gaattcttgg tttcggtggt tcaactgatc aagttaatga agctgttatt
    10561 caattatctc aaagtttctc aaacggaaaa gtagacgcac aaacttggaa ctcaatgatc
    10621 aacgctcaac ttggacctac tctttctgct attgctaaaa agatgggaat tacaatggga
    10681 gatctgaaag aaggtttatc tcaaggtaag atttctgttg aagaattcca aaatcaatta
    10741 atagaaatgg ataccaaagg tggcggagga cttaaatcat taagtcaaat cgctaaagat
    10801 tctactaagg gaattaaaac ctctatacaa aatgcaaaaa cagctgttac acgtggtgtt
    10861 ggtgaggtaa tagaaggatt aaataaagca ttagtagatt cagatttagg tggatttaag
    10921 ggaattattg ataaagtggg cagttcaatg gaatcgttct taaaagtaat tgctgcaaat
    10981 attcctacag cagtatcatt tttaagtaat ttatttaacg aaattcaaaa atttggttct
    11041 gctttgaaat tcatgatgcc ctttcttgtt cctgcagcta ccgcttttgg agcgttcatg
    11101 tttcaactta aaggtatacc agcaataata aaaagcttca ataattttaa gaatgccata
    11161 attggcgttg gaaattcatt aaagattatg ggggcaatag ctgctgctaa tccatttgtt
    11221 ttaattattg gtgcagtcgt tggtgcaata gctgttttcg gttactttat ggcaaccaat
    11281 gaagagctta gaaacaaggt tatatctatt tggaatgatg tgaaagattc cgtaattggt
    11341 gtattaaaaa atataaagga ctggggaatt gatacttgga attctgctaa agaaatggca
    11401 tcaaatgcag ttgagggtgt caaagatgct tggtcaggaa caaaggaatg gttttctaac
    11461 acctggaaag gtattaagga tggggccaca ggtttatttg ataaaacagt agagacttca
    11521 agaaatgcag tcgatagtgt aaaaaatgca tggtctaata tgaagaaatg gttttctgat
    11581 acttggcaaa gtataaaaga ttcagccaga gaaaaatgga atggaatcaa aggttctatt
    11641 atgtcagttg ctggtccatt gattacaggt ataaagaatg catttttaca tgttactttt
    11701 tatctagaga ccttatggaa taatttagtt gagattggta aaaacgtctt tgagatttta
    11761 aaaaatgtta tacttgcgcc ggttttgttt attacctcca tgatttctgg tggatgggaa
    11821 gaaacaaaaa ataatatgat tgctgtttgg aataatatta aagaaagtgc cataaatatt
    11881 tgggaatcta taaagaatat atttgtaagt tattttaca
//
LOCUS       DACDLI010000073        11870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDLI010000073 DACDLI010000000
VERSION     DACDLI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.073"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..2378
                     /locus_tag="IV353_002182"
     CDS             6..2378
                     /locus_tag="IV353_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010387.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYDKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..3899
                     /locus_tag="IV353_002183"
     CDS             2439..3899
                     /locus_tag="IV353_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9010388.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKDIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMMFQQGRQHERE"
     gene            3910..6114
                     /gene="topB"
                     /locus_tag="IV353_002184"
     CDS             3910..6114
                     /gene="topB"
                     /locus_tag="IV353_002184"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9010389.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKSTRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPNQYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAGETYS
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTTQNGTFDAKLVPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHAPNSRYVNNKKVQEHYAIIPTKMVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVILYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6360..6956
                     /locus_tag="IV353_002185"
     CDS             6360..6956
                     /locus_tag="IV353_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9010390.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6969..7868
                     /locus_tag="IV353_002186"
     CDS             6969..7868
                     /locus_tag="IV353_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9010391.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGVKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDLIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            7871..8356
                     /gene="ssb"
                     /locus_tag="IV353_002187"
     CDS             7871..8356
                     /gene="ssb"
                     /locus_tag="IV353_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9010392.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8722..8982
                     /locus_tag="IV353_002188"
     CDS             8722..8982
                     /locus_tag="IV353_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010393.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9430..9636
                     /locus_tag="IV353_002189"
     CDS             9430..9636
                     /locus_tag="IV353_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9010394.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKGKKR"
     gene            9636..9779
                     /locus_tag="IV353_002190"
     CDS             9636..9779
                     /locus_tag="IV353_002190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010395.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLVPRYWTIESRKH
                     GNL"
     gene            9829..10266
                     /locus_tag="IV353_002191"
     CDS             9829..10266
                     /locus_tag="IV353_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010396.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYAKFAQIKKAKVMASDWLDTFESIDKNGAFYMIESEEKNND"
     gene            10259..10777
                     /locus_tag="IV353_002192"
     CDS             10259..10777
                     /locus_tag="IV353_002192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010397.1"
                     /translation="MTKLEVKVKINYFHLLPTIENGETILRLETADYIMEVYMKKRNE
                     MERTLCYNLIEKKNESLTHHQLFRSALVDEKMKKLNQKPFVHYDYYVLFSQFLTQKSE
                     FVVVNGSGYILTVEKDVYLDYLEKMKNSSEEELEKRKELYQLYHFDEAEFLELELNKH
                     HILYQKFLSNQF"
     gene            11160..11339
                     /locus_tag="IV353_002193"
     CDS             11160..11339
                     /locus_tag="IV353_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010398.1"
                     /translation="MPIAIGNKRLPVTLDEKRQEELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11398..11751
                     /locus_tag="IV353_002194"
                     /pseudo
     CDS             11398..11751
                     /locus_tag="IV353_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4404 a         1982 c         2471 g         3013 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg tttgggaaaa tcaaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaatcga gaagatgttt gaacaccaga
      121 ggaagacaaa cggtcaacca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagaacgttt agaaaataaa gtccaaagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggttataac aagtacggtg
      301 gcctggatat gacgatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gctgccacga tccgaaaata ccaacgaaaa ttggaaacat
      421 tagaacagct gaaagaaaaa tcagagaaag gaaaagaaaa tattcttcct gaagtacaag
      481 cgatcattga tagcggacga gtgacgcaat ggaaaaagaa tccaactatc tattttctga
      541 aaggctatcg aaaagtcgct ttagaactca atgaaaacgg gggatttgag gaatccaaaa
      601 aataccaagc cacgtcggaa gaagaaaagg cagctgttcg tgaattactt gaagaagcta
      661 cacctactga ttctgtgaag gagaaagaaa ccgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agcaaaaggg ttgaaaagtt atggtgggga gatcgtaaca ctagaattga
      781 tcgatcaact caaaacactg gataaccaat acgccgcaga agcaggctat tataaattct
      841 attttgaaga agtgaagaat ggagaagtag tagatcagat acgaatggat ctaggggatg
      901 gcgtcaatag taatcacccc atctatttag agttagctag attgtgcgaa caatcacctg
      961 aaaaggaaca agaacaaaca gtcaaaaatg aacctatttc aaaagtacaa aatgagatta
     1021 aagagacaaa aacaaaacaa aagggttatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc actttcccaa caggcgcttg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtcaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat cgaggaacaa tttccaggcg caacggcttg tttaggtcgt gccgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgat aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac aattgaaacc tgaagactat ccgaaaatct
     1501 tcccgaaccg cgtctttgat tttcaattgg atcaaacagg gcagacagaa cttcaagttg
     1561 ggattcaagc ggtagccaaa aatattggaa ttcaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacgtg gggcgtacgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagcc acaagtattc
     1741 acgaattagc ccatgctaga atgcacaaat tttcagaatt agatactgca acaaaagaac
     1801 tacaggcaga aatgacctcc tatattgtgt gcaagcattt tgggatggac acttcggaga
     1861 aagctattcc ttatattgcg acatggacga agaatggtca gacgttggat gataaggaag
     1921 ctgcggaacg tgggaaaatc atgaatgatg tcagtcgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgaagcagga gcataaaaat actgtagaaa cggcagaaca aaagaatatt gtgcccgttg
     2101 gctcactttg gatcgacaag aaagacggga aagtcctgga agaagacacg ggccgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg attttaaaag
     2221 gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gtttgaaaat gacagagaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tagtcaataa aggacgagac tatcgcttag aagaccatga ttcttttgtt tttgatcgaa
     2581 gaaaacaacg tttttattgg aatagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt catagacaaa gatatccaag accctaagca acaatttaaa gcagccactg
     2701 attttattct aaagaatgaa gacaaaacag aacgagtgga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga aggaattatc
     2821 tgaaggaaga acgaaaactg cccgattggt tgattgatta tgcagaaaaa gaaggattga
     2881 ttgccgaact aaagccaaaa catgaacggc aaaatttctt agttcgagac gatcgtttgg
     2941 atcatgcggt cgccttttta tggaaagatc cacagacaaa agagacagta ggggcgagtt
     3001 atcaaggaac gttcattgat tatgaacgtt ttggtgagag aggcacatat aagcatatag
     3061 ataaaaattc gacagcgaat catggcttta acctaaagat tggtgatcct aaacagttaa
     3121 aattttttga gagtagtatt gatttactta gctacgctgc actgaatcgt gatcagttaa
     3181 acgatacgtg gttagtctct atggaagggt taaaacacca tgtgatcagt cattattttg
     3241 gtgaggcagt atctgaattg agaaagaaac aagcctttcc tcaatccatc gagatttgtg
     3301 tggataatga ccgtgcagga catattttct acgaaaaaga acaattaatg ggcgcagtcg
     3361 atccatttac gaatcagaaa gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc
     3421 caagagaata caaagtcatt tatgaagaag tcgcaaaaga agaaaaggtc acaccagaag
     3481 ctattatggc catacacaag acagaaaaca atctgcaatt aacgaaccaa ctggtttccg
     3541 ctcacaaagt aaatgcgtct tttggccagc agctttctgt taacgattca atagaagcca
     3601 ttaatttgaa agatatttgt agagaagtcg caaaagaatt aaaagcttgt gaacgcgtgg
     3661 atggtacgta cgattttgat cgtttttatc aagaaaaagg ggatattaat gcgcagattc
     3721 ttttctctta taaagcagaa caatactaca aaggctataa aaatcatgaa catgaatttg
     3781 tacctgaagt aaaaaaggac tggaacgatc aattgaagca tgagatccag caacaagaaa
     3841 tcagaaagca aaaacgtgcc atgatgtttc aacaaggtag acagcatgaa agggagtgaa
     3901 atcgtgatga tgaaaaccgt tattttagca gaaaagccgt cacaagcgaa agcttatgcc
     3961 gatagcttct ctaaatccac ccgaaaagac gggtattttg agatccaaga tcgattgttt
     4021 cctggtgaaa ctgtgatcac gtatggtttt ggtcatttag tcgagttaga ttcgcccgat
     4081 atgtacgatg aaaattggaa acaatggtca ttagagcatt tgcctatttt ccccaatcaa
     4141 taccactatc atgttcctaa agataagaaa aagcaattca acgtggtgaa acagcaactt
     4201 caatcggccg acaccattat tattgcgaca gatagcgatc gtgaagggga actgatcgct
     4261 tggacgatca ttcaacaagc aggcgccgat caagggaaaa cctttaaaag actttggatc
     4321 aactccttag aaaaagaagc catttatcaa ggattccagc agttaagaga cgcaggagag
     4381 acctattcaa aatttgaaga agcacaagca cgtcagatcg ccgattggtt aatcggtatg
     4441 aatggcagcc cactgtatag tttactcttg cagcaaaaag gcataccagg aagcttttcc
     4501 ttaggtagag tccaaactcc aacgctttac atgatttatc agttgcagga aaagattaga
     4561 aacttccaaa aagaaccata ttttgaagga aaagcccaag taaccactca aaatggcaca
     4621 tttgatgcga agcttgttcc gaatgaaacc caagcgacgc aagaagcatt tgaagcctat
     4681 ttaaaagaaa aaggtgttca agtaggaaag cagccaggca cgattcttga agtcgaaacc
     4741 gagaaaaaaa gcgcggcaag tccacgtctt ttttctctat ccagccttca gtctaaaatg
     4801 aatcagctta tgaaagctag tgccaaagac acattggaag ctatgcaagg tttatatgaa
     4861 gggaaatatc taagctatcc gcgaacagac accccttata ttacggaagg ggaatatgcg
     4921 tatttgttgg atcatttaga tgaatacaag cattttttaa aggccgaatc aatcccaacc
     4981 cctattcatg cgccgaatag tcggtatgtg aacaacaaga aagtccagga acattacgcg
     5041 atcattccta caaaaatggt tatgacagaa gcaacgtatg aaagtctgac acctttacaa
     5101 caagcgattt acgagcaagt tttaaaaaca acggttgcga tgtttgcgga aaaatatacc
     5161 tatgaagaaa ctacgatttt gacacaagtt cagcagcttc agttaaaggc aatcggaaaa
     5221 gtaccgatag atctaggttg gaagaagtta tttggcaaag agagcgacgg aaaagagaag
     5281 gaagaagagc cgctacttcc taaagtgaca aaaggagaaa tggtgacggt agatctacaa
     5341 gtccttgaaa aagaaaccaa gccaccgcaa ccttataccg aaggtacgtt gatcacggcg
     5401 atgaaaacag ctgggaaaac cgttgatagt gaagaagcgc aatccatatt gaaagaagtg
     5461 gaaggaatcg gtacagaagc gacacgggcg aatattattg aaaccttgaa acaaaaggaa
     5521 tatatcaagg tagagaaaaa taagctcgtg gtgacgaata aagggatttt gctttgccaa
     5581 gccgtggaaa aagagccgct gcttacgagc gccgaaatga ccgccaaatg ggaaagctac
     5641 ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt tcctagccaa tatccaaaaa
     5701 tttgtctctc acttattgga agtcgtacca gggcaaatcc aatccactga ttttggatcg
     5761 acgttacaag aagtgaaagc agccagtgaa aagcaagaag ccgcgcgtca tttaggaatt
     5821 tgtccaaaat gccaggaaca agaagtcata ctttatcaaa aggtggcggc ttgcacgtct
     5881 gaagcctgtg atttcaaact atggacaacg attgctaaaa agaaacttac agcgacacag
     5941 ctgaaagaga tcattcaaaa tggccgaaca agccaaccag tgaaagggtt aaaaggtcag
     6001 aagggttcgt ttgaagcaac gattgtttta aaagaagact tcacaacgag ctttgaattt
     6061 tctgaaaaga aaaaaacgaa ctataagaaa agaacacgac gcacgacaaa ataatggagg
     6121 gtttatctta tgcgaaatac aacacacaaa caacaagaaa tcgaaaatca aaaagccaaa
     6181 attcgttcag aacaggaaaa aatgcgtgcc agcaaacgaa gactgcagca attgaagaac
     6241 cagcagagga ttcaaaaaga aatcgaatta gatcgataaa atcttcctaa ctattgtata
     6301 cgtataaact tatgttatac tgtttttata agtttatacg tatacaaagg agggagaaaa
     6361 tgacaaaagc aatggtatac aagctgctag aagaatttaa tggaactttg gctttgaagg
     6421 atgccaaaaa agcgggcatt tctcctgtaa cgatcaaacg tatggtagat cgaggggagc
     6481 tggatcgaga gtatccaggt tttttcacct tacctggtca atttcctgat gaattgttta
     6541 tggcgcaaaa aaaatatgaa cgcggcatta tttcacatat taccgcgtta gatttgtatg
     6601 atttaacaga tatgatccct agacagatcg atctgactgt accttacggt tatcacgttt
     6661 ctgaaaaagg attgaaggaa ttcgcagttg agctacacta tacaaaagcg gaatggtatg
     6721 aattaggtaa gattgaaata aaaagtcgct atgggaatcc tattatagct tatgatccag
     6781 aaagaacttt atgtgacatt tggaatccct ggtataccgt tgaggatgaa atcaaagtaa
     6841 aagcaatcaa aaactatatg gagtcggata gaaaaaatct tagaaaattg aatgagtatc
     6901 gacgaatttt accaacagac aaaacgatgc gttcttatat tatggcttta aattaaagga
     6961 gaagatacat gacacctacg cagttaaaag caaaagtgaa gaatatttct agagaaaaag
     7021 aagttgatcc gcagcttgtt ttaagacatt ttatgatgga aaaattcttg gaaaaaatct
     7081 cagagtctcc ttatcgtgaa aattttgtgt taaaaggcgg cttcttgatt ggctcaaagt
     7141 atggcattga aaatcgcaca accaaagata ttgatacgac tttacgggaa atgaaagtaa
     7201 ccaaagaaac attaacaacg gttctaaacg aaattttttc aacgccgacg aaagaaggca
     7261 ttcaatttga gattcaagga gtgaaagaaa cgagagaagc cgattactat ccaggattta
     7321 gcttacgtgt gctggcacat ttggaaaaca tgcggccaga ttttaaggta gatgtgacta
     7381 caggtgactt gatctatcct gcaacgatca ctcacagtca taaattgatg tttgaggatc
     7441 gaacgattcc tttggaatcc tatcctacag aacaaattat tgctgaaaaa ttaagtgcaa
     7501 cctttgattt tttaacagat aattcgagag gaaaagattt ttatgatctc tatactattc
     7561 ctaaaatgga aaagatcgat gagaagacct tgaaagatag tgtgagaaat acatttacac
     7621 gtcgaggaaa aactgatccg ttaaagattt attacgaaaa ggatatggat gtactgaaaa
     7681 atagtcatga gctaaaagat acctggaaga aatatcaaaa aagcaatcct tatgctgcgt
     7741 cgatcacgta cgaagagaca atggattcga ttagtaattt gatggaaaaa gtgattcacg
     7801 tcgaagaaaa agagcgaaaa atagcttatc aaagacacca acagatgaac agagaaatgg
     7861 agagataaaa atgatcaata atgtaacttt agtaggacgg ttaaccaaag atccagattt
     7921 acgttatacc gcaagtggca cagcagtggc tacttttacc ctagccgtta atcgtaattt
     7981 cacgaatcaa aacggcaatc gagaagctga ttttatcaat tgcgtgattt ggcgcaaacc
     8041 agcagaaacg atggcgacat tagctaaaaa aggcagcttg atcggcgttg tcggacggat
     8101 tcaaacacgg acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtagc
     8161 tgataacttc caactattag aaagcaaagc agcgacagag agccgtgcgc acgctgatca
     8221 atcgtccacg agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaacaa
     8281 caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata ttagcgatga
     8341 tgatctaccg ttttaagtag cattttctat tggttgcatt tgctacggtt tatgttataa
     8401 tttagtcaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg cgggctagtg
     8461 gttttctttt acgtttctat tataagaatt aacgggtagg cttactgtct ttcttggcaa
     8521 atttacaatc aagccatttg cagatatagt gcgcggctac ttctgccatg attgcaaaaa
     8581 caaaatcaaa aagaattttc aaacctataa cctcctcccc ttgccagatt aggccggggt
     8641 agcagacagc aagactatta tagcataatc gaaatatcca aatgattgga tttttgctat
     8701 aattttagcg aggtgattgt gatgaacaaa tatgttggtt tgcttgataa aatccgagta
     8761 ataaaaacgc aaccgttatt ggttcgcttt actttacaaa cgattcatga atcgattaat
     8821 tgtgtagttg cagatattga aattatcgat aaattgctaa ttatggatga tggaaaatac
     8881 aatattgccg tcacaggtca cttcaataaa cgaaatcagc tagttattgc tagcatgtat
     8941 gttagaaatc ccgatcattt tacgcgatcg atgggcatat aaaaaaagaa gagttgcaat
     9001 cctaatgtag gactgcagct ctttttgttt tacatagaaa caggaagaaa gggaaaggcc
     9061 tgtttaggaa ggcacaagcg ccttctcaat tgaaaaacct ttctctcact ccaaccgcca
     9121 ccgccgttcg agaagaaagg aagtgaacgt tgcacgttca ctaagataag gggtcttgct
     9181 ttgcaagaca aaataggaaa agcttcctct caccaaaagg ttcgttcctg ttcgctatct
     9241 ctttattgat ttgccagacc ggcaacaagt tgccttttat caccaaaaag cgacaaggac
     9301 aagggtaaat ggcacgctgc gcgcccttgt ccttgtcgct tttcgttgat tcattctggc
     9361 caacaaatca ataaagaaag aactaacgaa aggaaataaa acctttccta agaggaggca
     9421 gaatcaatca tgcgaaagaa atttgattat tggggtgttc ctttcagcga attattccca
     9481 aactatcatg caccacatac tgtcgaatgc gattgtggag aaagagcgaa atgtatcaaa
     9541 agctatcgtt tgtatcaatg tccaacatgc gggaaaaaat ataccttgtc ttatggcgat
     9601 tatgttttga ttgatgagaa aggaaagaaa cgctgatgaa aacacaacac aaattattga
     9661 ctgtaaaaga agtcgttcaa attttggtgg acaaagaagt catttctgaa aaagtagcag
     9721 aagctttact tgttccgcgg tactggacga tagaatcaag aaaacatgga aacttatgaa
     9781 aacgaatatt ggttaaccaa attagagaac taaaaaggag aaaaaaatat ggctcaaaat
     9841 tactatgacg aatttgtcaa attacctttg gataaaatgg cacaaaaaat ggaggatatg
     9901 acgttcctat accatgaaac acgtgtaccc aaaaaacatt acaaggaaaa attgtcagtc
     9961 gcagtggaag aaatgattga atcaggtgtc gaaatgaatc tcattgcgac gtactatcga
    10021 acattggaag aactgaaaaa acaaaatgga aaatggtttt tccaagcctt actatgcttg
    10081 gaagcaggcg tcaagccaaa cactatcaaa ccttctgaat atcaagcctt agaactaacg
    10141 tatgcaaaat tcgcacagat taaaaaagcc aaagttatgg ctagtgactg gttagatact
    10201 tttgaaagta tcgataaaaa tggtgcattt tatatgattg aatcggagga gaaaaacaat
    10261 gactaaatta gaagtaaagg taaaaatcaa ttattttcat ttattaccca cgattgaaaa
    10321 tggcgaaacg attttacgat tggaaacggc agactatatc atggaagtat atatgaaaaa
    10381 acggaatgaa atggaaagga ctctttgtta caacctgatt gaaaagaaaa atgaatcact
    10441 aactcatcat caattgtttc gttcggcttt agtggatgaa aaaatgaaga aattgaatca
    10501 aaaaccattt gttcattacg attattatgt tcttttcagc caatttttga cccaaaaaag
    10561 tgagtttgtt gtagtgaatg gttctggata tatcctgact gttgaaaaag atgtttattt
    10621 agattatcta gaaaaaatga agaattcgag tgaagaagag ttggaaaaaa gaaaggaatt
    10681 atatcaactt tatcattttg atgaagcgga attcttggaa ctagaattaa ataaacatca
    10741 tattctgtat caaaagtttt taagtaacca attctaaaag catttatacc gaaaagtgag
    10801 aatgtgtgaa ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca
    10861 atcgctccaa ccgcccccgc cgcttaggtg atgcgaaata agcgggcaaa gcccgataac
    10921 ctaagagaaa gaaggagatc catatgaata aaatcattcc tctgttgttc gtactgtgta
    10981 ttggcgagat acttatgcgt catggtgcag ctgtacattt aaacttcgta gtattcttag
    11041 gaatactagt attttgtttg attttttggc gctttgtgtt agcccttgtg agtctactat
    11101 tcaagttgat tgcagctata tttttcatag gattatttcg atgaaaggag agagtaccaa
    11161 tgccaattgc aataggaaat aaacgtttac ccgttacgtt agatgaaaag agacaagaag
    11221 aattgcagca actaaagcag aagtacggaa aaagtgaatc taagattatg tgtattgcgt
    11281 tagatctatt gatcgcgcaa gaaaaagcag gatttgatgt accagcacta aaaaagtgac
    11341 gtcacttttt tatttgattt atataagtga tatcactttt agcaaggagg gaaataaatg
    11401 ttaaaaatta aaaagaattc gtacaaatta aagcttagta tactatctat tatttgtctt
    11461 gtaggcatga taattagtgg tcatttgtat agagtggcat atacgcagtt tattgagttc
    11521 aaggttcact ctttaggatt agctggggat cgatacgttg aatatgtcca ttattcatta
    11581 aaatgttctt catatattct tctttggctt attttttcat tgtttgaatt gctaaattta
    11641 atagcaatat cttcatatca cttagataag ctaaatacca aaaatagccg ttataacgaa
    11701 gtgttcaagg aaaatatgaa gttagaaagt gaattaaaca agctaaaaaa aataaaaaga
    11761 agaaaacata agttaactaa aattatttta ttaagaatga tggaatataa gggtaatgct
    11821 accaacataa cgatcattat ttctctgata tgctatgggc ttctatcaat
//
LOCUS       DACDLI010000074        11652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDLI010000074 DACDLI010000000
VERSION     DACDLI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.074"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(206..547)
                     /locus_tag="IV353_002195"
     CDS             complement(206..547)
                     /locus_tag="IV353_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010399.1"
                     /translation="MLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLIRKV
                     VELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVKSTILCKS
                     STSSKAQCFAL"
     gene            complement(983..1342)
                     /gene="rplT"
                     /locus_tag="IV353_002196"
     CDS             complement(983..1342)
                     /gene="rplT"
                     /locus_tag="IV353_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9010400.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1395..1595)
                     /gene="rpmI"
                     /locus_tag="IV353_002197"
     CDS             complement(1395..1595)
                     /gene="rpmI"
                     /locus_tag="IV353_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9010401.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1667..2191)
                     /gene="infC"
                     /locus_tag="IV353_002198"
     CDS             complement(1667..2191)
                     /gene="infC"
                     /locus_tag="IV353_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9010402.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2511..3143
                     /locus_tag="IV353_002199"
     CDS             2511..3143
                     /locus_tag="IV353_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9010403.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(3145..4050)
                     /locus_tag="IV353_002200"
     CDS             complement(3145..4050)
                     /locus_tag="IV353_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9010404.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(4135..4788)
                     /locus_tag="IV353_002201"
     CDS             complement(4135..4788)
                     /locus_tag="IV353_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9010405.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            5110..6054
                     /gene="mvk"
                     /locus_tag="IV353_002202"
     CDS             5110..6054
                     /gene="mvk"
                     /locus_tag="IV353_002202"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9010406.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            6047..7024
                     /gene="mvaD"
                     /locus_tag="IV353_002203"
     CDS             6047..7024
                     /gene="mvaD"
                     /locus_tag="IV353_002203"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9010407.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            7037..8122
                     /locus_tag="IV353_002204"
     CDS             7037..8122
                     /locus_tag="IV353_002204"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9010408.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            8119..9183
                     /locus_tag="IV353_002205"
     CDS             8119..9183
                     /locus_tag="IV353_002205"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9010409.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9432..10235)
                     /locus_tag="IV353_002206"
     CDS             complement(9432..10235)
                     /locus_tag="IV353_002206"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9010410.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(10255..10560)
                     /gene="kduI"
                     /locus_tag="IV353_002207"
     CDS             complement(10255..10560)
                     /gene="kduI"
                     /locus_tag="IV353_002207"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9010411.1"
                     /translation="MGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRVFHMMG
                     KPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQMDQLK"
     gene            complement(10542..10748)
                     /locus_tag="IV353_002208"
     CDS             complement(10542..10748)
                     /locus_tag="IV353_002208"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP9010412.1"
                     /translation="MLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVIIIGG
                     ILTGIFTAVEASAIAVIYKWVIRF"
     gene            complement(10792..>11652)
                     /locus_tag="IV353_002209"
     CDS             complement(10792..>11652)
                     /locus_tag="IV353_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9010413.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT         3430 a         2422 c         2183 g         3617 t
ORIGIN      
        1 tccatcactc ggataattgg aacacttttg atggaaaaag acactgagtg gctggcttct
       61 ccacgaaaat atttagaatt taattcgaat aacatttagc accatgaacc tgtgcgagtc
      121 tcttctttct gaggagagac atcaagagtc gctgcgctac tgctcctgac ctctctcctc
      181 agaaagaaag gcatggcagt taggttcaca acgcaaaaca ttgtgctttt gatgaggttg
      241 aacttttaca caagattgtg gacttgactg aaatacctaa tgacatcaag atcggcaaca
      301 caataactgc aatagaaatc actggtccga ttcctgtcat cgacatgaat ataacagcgg
      361 ttacaataca tagtaagctc atcgtgattc ttggcttgtc ccctccaagt tcaactacct
      421 ttcgaatcag ggtagctgcg attccgctat ctactagaac acgcccgaaa aaggcaccaa
      481 agaagacatt taccaagatc gatttggcgt actcagctgg ccccgtcgta tagatatgcg
      541 tcaacacatc aattactcct tgcccttcca tcgccgagtt tggtgaaaac gtgtttccga
      601 ctagcgctaa tcccatccat acagtagcca tgatgacaaa agccgaccat caagttgtag
      661 ccttttacac agtaccagac catgaaaaag aatgagataa ctaacaaaat accaataata
      721 atatcactca tgttattcct ccgtccattt tgtttttctg ataagcaaac taatatatca
      781 gtcaaactca ttctaactta tgtagttagc gctttcatta aaggatgacc ataaatgttc
      841 agttttcaat taaaaaacct taattatgtt attttagact tatacgttat tgtcctgttt
      901 catttgatgg aggattgtaa ataaaaaaag aggaaagaac ataacttttc gttatcttct
      961 ttcctcttta taattgttta gcttatttag ccaaagcttc ttttgcttgg tcagccaatg
     1021 cagtaaatgc tgctgcatcg ttaacagcca agtcagctaa cattttacgg ttgatgtcga
     1081 tttcagcaac tttcaaaccg tgcattaatt ttgagtagct caagccattc atacgagctg
     1141 ctgcgttgat acgagcaatc cacaatttac ggaagtcgcg tttcttttga cgacgatcgc
     1201 gatatgcgta gttgtatgaa ttcattactt gttcttttgc tgttttaaat aaagtgtgtt
     1261 ttgaaccgta gtaacctttt gctaatttta gcactctttt acgacgttta cgagttactg
     1321 ttccaccttt aacacgtgcc atgtataatt cctcctaata aaaataaatc ttcagattct
     1381 gttatttgta cttcttattt catttttgct agttgttggc gaatacgttt gaagtcgcca
     1441 ctagaaacca tgcttgcttt acgcaattga cggcgttgtt tttttgtttt gccgtggaaa
     1501 cggtgacttg taaacgcgcg gaagcgtttc aatccgcctt taccagtacg tttgaaacgt
     1561 tttgctgatc cgcggtgtgt tttttgtttt ggcatgacta tatttcctcc tcaaaatctt
     1621 tcgttatccg actatcggct gtccaacagc cagttagtca ttcaaattac ttactgtcgt
     1681 ttttcggtgc cagcgtcaag aacatgctgc gtccgtccat tttcgctttt tgttccactg
     1741 ttgcaatatc cgcagtttct tcagctaagc gattcaagac tttttgacca atttctttat
     1801 gggtaatggc acggcctttg aaacggatag aagctttcac tttatcgccc ttttccaaga
     1861 acttacgtgc attacgaagt tttgtattga agtcattcaa gtcgatcgta ggacttaagc
     1921 gaacttcttt tacattgatc actttttgtt ttttacgcgc ttcgcggtct ttcttttgtt
     1981 gttcgaaacg gtatttaccg tagtccatga ttcgcgcaac tggtggtttc gcaccagggg
     2041 caactaacac aagatccaag tttgcttgtt ctgcaatatt caatgcttct gcttttgtct
     2101 taactcctaa ttgctcacca tctgaaccga tcaatcgtaa ctctcgtgca cgaatgccgt
     2161 cgttaaccat catatccttt gctatggtca ttcacctcca aatattatga gagaaagaaa
     2221 agcttaccga ataaacggca ataaaaaaac gagttctctt acagaacccg cgcgtacttc
     2281 acctaataga accagtgaaa actatctagc ccagtgacat tatcaactaa ggcgagaagc
     2341 gggtgcttct gcttttattt ctcaaccatt atagtatagc aaaaactaat ctttttgtca
     2401 atgtttttca tacagtttgt cttccttttc tgaaaatgat tagattagca ggctttctag
     2461 cagtgattcc cgcttctttg ctatactaaa gaaaaacgag gtgaacccac atgaaaaaaa
     2521 tcgaacctat tccaccttct cttcctcttc ttacaaaggg agagttttat ttagaagatg
     2581 aggagatctt ctctggtctt ctgatcaacg gaggtaacca aagctatctc gatgccaaaa
     2641 atatggtatt aagagagagt catatccaaa aacttacgat gcaaaaaaca ggtttacgtc
     2701 attttgaatg cagtaatgtc atttttgaaa aatgtgattt ttccaatctg gagtggattg
     2761 gtggcagttt ccaccaagtc gtgtttcatc aatgtaaatt aacaggtacg aattttgccg
     2821 aaagttatct tcgagactgt actttcactg attgtattgc ttcgatggct tcctttagta
     2881 gtacaaactt gaaagctgtc tccttcgatc attgtcaatt agaagacagc gaattttatg
     2941 aggtcacttg gaaaaacctt tttttgtctg acaatcagtt aaccggttcg aactggtttc
     3001 gaacttctct gaagggatta gactttacga ctaatttctt cacaacaatc gctttatctc
     3061 aagattcttt ggcgggacta atcgtcaatc aagagcaagc acttgtgatc gcccaagccc
     3121 ttggtttgca gatcaaagac tagatcaacg aatccgccgc cacagtgcaa taagcatcca
     3181 aatagataag accaaactga tgatcaaggc gatttcccat ccgaaaacgg aatgggtaaa
     3241 gggtaactgg agattcattc cgaagaatcc tgtcacaata gttggaacgg tcaagatcag
     3301 cgaccagacc gtcaaaaatt tcatcgtgtc attcaagttg ttattcagca aattattata
     3361 agtgccagag agctggtcta aaatctgcga agctaagttc gtcatttcaa ctgcttgtcg
     3421 tgcttcgatc agtgcatcgt ccaactcttc tttttccttt tctgacaact tgcgataaat
     3481 agccagtgcc ttgatttgtt ctaaaagtac tacattttgt tttgttcctg taactaagta
     3541 aaccaaaccg atctctaaat ctgaaagttg cagcagattc ttgttcgttg ttttttctct
     3601 taatttcaga ttaagccgct gacgttcact attcacttcc tcgattaaag gaaaaaagga
     3661 atcagaaatc aaaaacaatg tacgaaacaa taaactgtat acactcatgt ctggcatccg
     3721 ttccaaaagc gatcgaatta gtcgaatcac gtactcggta ttcggcgtgg taaatgtaaa
     3781 caaaccgtct tcttttaaaa taaaagcgat aggactagtt tcataatgat tatcgacttt
     3841 ttgctggtgc ggaacatttg atacaagcag taacgcttca tcaaaagaat cgtactctac
     3901 gcgcgcccgt tcatttttat ctagagaata agtcagcatc tcgtcactaa tgccatattc
     3961 ttcttttaag tttttaacag ctagcacatc gttttgctgt acattgatcc aaaaattacg
     4021 gtcttgatta aaatagtgtt tttcccgcat actctatcct cctgacattt gtttccagta
     4081 taaagaaaaa agtcgctcaa ataaaggttt accctttatt tgagcaactg taaatcaaat
     4141 ggatactttg gtttggcgaa tctctgctaa tttttcttcg atcattttca agacttcttc
     4201 ttttgcttga ggatcttcca caaaattata gcggtctcca tcgatttgga tttttggact
     4261 ttctttgtat tcttcatacc attgatcata acgtctgttc aattctttgt aataatcgta
     4321 tagacttggg tcaaaggaca attgttcata cgaacgacca cgtttttcga tacgctctaa
     4381 catcgtatca aaagatacac gaatatgaac taataaatcc ggatgttttt tatgggcagc
     4441 atagggaagt tcttccatca tgttttccag cagttcatca tacacacaaa cttctgtttc
     4501 tgttgctctt cctagatctg catttagatg gaaaagcagt gaatcttcat aaatcgaacg
     4561 gtccaataca ttattgtcat ctttcattgc ttgtttgatg cttgcaaaac gtttattcaa
     4621 aaaataaatt tgcagcaaaa aagcatactg ttcagggttt gcataaaata agggcaagac
     4681 ttcattatca tcaatcgatt cataaaatgc ctgactatca aagtgttcag ccatcatttc
     4741 tgttaaactt gattttccgg caccaattgt acctgctaaa acgatcacgc tcatttacct
     4801 ccagctcact tcatcactaa atattgtgtt taactccaac cttttttaaa tataccccca
     4861 atatctagta gtgtcaattg tattttttct agtcaaagaa gaaaaaaagt caaaaaattc
     4921 ttaagaaaaa agtgtttttt ctttagaaaa cgtattctta gttttacttc tgagatggtt
     4981 ttagtaaaat aaaggaggaa tgttttttga gacccttagt ggacaaaaaa attcgtgaag
     5041 cagcgcaact cgttttgggc gctttatttt ttgcttaaaa atcactttta cgtgagaagg
     5101 agttagatta tggcaaacta tggccaagga gaatcaagcg gaaagatcat attgatgggc
     5161 gagcacgcgg ttgtttatgg agaaccagcg attgcctttc ctttctatgc aacaaaagtc
     5221 accgcattcc ttgaagagct ggatgcaatg gacgatcaac tggtttcttc ctactattca
     5281 ggaaatttag ccgaagctcc tcatgcatta aaaaatatca aaaaattatt cattcactta
     5341 aaaaaacagc atgacatcca aaaaaacttg caactgacca ttgaaagcac gattcctgct
     5401 gaacgtggaa tgggatcaag cgctgcagtc gccacagcag tcactcgtgc tttttatgat
     5461 tacttagcat ttcctttgtc tcgtgaaata ctattagaaa atgtccagct ttcggaaaaa
     5521 atcgcccacg gtaatcctag tggaatcgat gcagccgcta ctagcagctt gcagccgatt
     5581 tattttacaa aagggcatcc ttttgactac ttttctttga acatcgatgc ttttttgatt
     5641 gtcgctgata caggaatcaa aggacaaaca agagaagccg tcaaagatgt cgctcacctc
     5701 tttgaaaccc agcctcatga aactggacaa atgatccaaa aattaggata cttgacgaag
     5761 caagcaaaac aagcaatcat tgagaattca ccagaaacgt tggcacagac aatggatgaa
     5821 tcacagtcac ttctggaaaa gctgacaatc agcaatgatt ttcttaatct attgatccaa
     5881 acagcaaaag ataccggggc attgggtgcc aaattaactg gcggtggacg cggtggctgt
     5941 atgattgcat tagcacaaac aaaaacaaaa gcccaagaaa tcagtcaagc acttgaagat
     6001 gcgggtgctg ctgaaacttg gatacaagga ttaggagtac atacctatgt ttaaaggcaa
     6061 agcacgcgca tataccaaca ttgctctaat caaatattgg ggtaagaaaa atgaagaact
     6121 catccttcca atgaacaaca gcctttcgtt aacattggat gcattttata cagagacaga
     6181 agtcatcttt tctgatagct atatggtaga tgaattttat ctagatggca ccttacaaga
     6241 cgaaaaagca acaaaaaaag tcagtcagtt tcttgacctt tttcgtaaag aagccggcct
     6301 ctcactaaaa gcttcagtaa tcagtcagaa tttcgttcct accgcagctg gattagcctc
     6361 ctctgccagc gggctagctg ctttagcagg agcttgcaat actgctctta agcttggatt
     6421 agacgatctc tctctttcaa gatttgctcg acgcgggtct ggttcagctt gccgaagtat
     6481 tttcggtggt ttcgttgaat gggaaaaagg ccatgacgac ttaagttctt acgctaagcc
     6541 agtcccttcc gattctttcg aagacgattt agcaatggtt ttcgttttga tcaacgacca
     6601 gaaaaaagaa gtgtccagca gaaatgggat gcgtcgaaca gtcgaaacat ccagttttta
     6661 tcaaggctgg ttagattccg ttgaagggga tctatatcaa ttgaaacaag caatcaaaac
     6721 aaaagatttc caacttctcg gagaaacgat ggaaagaaac ggactaaaaa tgcatggaac
     6781 gactttagct gcccagcctc cattcactta ctggtctcct gacagcttaa aagcgatgga
     6841 tgcggtgcgg caattgagaa aaaaaggcat tccatgctac tttacgatgg atgctggccc
     6901 taatgtcaaa gttttagtgg aaaacagcca tttatctgag gtacaagaaa catttaccaa
     6961 attatttagc aaagaacaag taattactgc ccatgctggt ccaggaattg cgattattga
     7021 ataaagggag atttttatga ttgaagtatc tgcaccaggc aaactctata ttgccggaga
     7081 atatgcagtt gttgaaacag gacatccagc agttatcgct gcagtcgatc aattcgtaac
     7141 agtcactgta gaatccgcac gaaaagtcgg aagtatccaa tctgcacaat atagtgggat
     7201 gcctgtacgt tggacaagac gcaacggaga attagtttta gatattcggg aaaatccttt
     7261 tcattatatc cttgctgcta ttcgtttgac tgaaaagtat gcacaagaaa aaaacattct
     7321 tttatccttt tatgatctga aagtaacaag tgagttagac agttcaaatg gccggaaata
     7381 tggattgggt tctagcggtg ccgtgaccgt tgcaacggtc aaagccttga atgtttttta
     7441 cgcgttgaat ttatctcaat tggagatttt caagattgca gcactagcca atttagcagt
     7501 tcaagataat ggttcctgcg gcgacatcgc tgctagctgt tatggtggct ggatcgcttt
     7561 ctcaaccttc gaccatcctt ggctccaaga acaagaaact cagcattcta tcagtgagtt
     7621 acttgccctg gattggccag gtctatccat tgagccattg attgctcctg aagatttacg
     7681 tttattgatt ggttggacgg gtagccctgc ctctacttct gatttggtcg atcaagttca
     7741 ccgttcgaga gaagacaaaa tggtggctta tcagcttttc ttaaaaaaca gtacagaatg
     7801 tgtcaacgaa atgatcaaag ggtttaaaga aaataatgta acgttgatcc aacagatgat
     7861 tcgaaaaaac cgacaattac tgcatgattt atctgcaatc actggggtcg tcatcgaaac
     7921 gcctgctttg aacaaattgt gtaatttagc tgaacagtat gaaggagccg caaaatcttc
     7981 tggtgcaggt ggtggcgatt gcggaatcgt aattgttgac cagaaatctg gcatccttcc
     8041 tttaatgagt gcatgggaaa aagcagaaat cactccactg ccgttacatg tctatagcga
     8101 tcaaagaaag gaaaaccgat gaatcgaaaa gacgaacatg tttcactagc taaagccttt
     8161 catgataaac aaaaaaacga atttgacttc gtacggatca tccataaccc tttgccgcaa
     8221 atagcagttt ctgatgtcga tctaagtaca caggcagttg ggtttacact aagcagccca
     8281 ttttatatca atgcgatgac tggcggaagc gaaaaaacaa aaaaaatcaa ccaagatctt
     8341 gcaatcgtcg caagagaagc agatttgatg attgccactg gttcagtcag cgctgccttg
     8401 aaagaccctt ctttagccga tacttatacg attatgcgac aagaatatcc tcacgggaaa
     8461 atcattgcca acatcggtgc agggacttct gtcgaaagag cccaagaagc tatccgattg
     8521 tttcacgcag atgccttaca gattcatttg aatgcacctc aagagttggt tatgcctgag
     8581 ggagaccgtg attttaccaa ttggaaagta cttattcaag agactcaaac agccattgac
     8641 gtccccctca tcgtaaaaga agtcggtttt ggcatgacaa gagaaacgct caatgatcta
     8701 gctgctttag gagttcatac agttgatatc agcggtcgaa gcggaacaag cttcacccag
     8761 atcgaaaacg cccgtcgctc caagcgggaa ctgagttatt tagctgactg ggggcaatcg
     8821 acagtctctt cattgcttga agcaaatgaa gcagacactt ctatggaaat cctagcctcc
     8881 ggaggtatcc gcaatgccta tgacatattt aaagcccttt gtctcggcgc aaatgcagtg
     8941 ggtacttcag gaaccgtact gacgcatctg atgaatcacg gcgtagaaga aactattata
     9001 ctgatgaagc aatggcaaga agagcttcgt ctgctttata ctatggtcgg cgcaacgaat
     9061 accgcggccc tacaccaaca atcactgatc ttttccggac cagtgaagga ttggtgcgaa
     9121 gcaagaggga tcgacttagt gaagtatgga agacgcacag aaaagagcgt gggacaaaag
     9181 taaagaacac ttttatccca cgctctaaac acgtataaac ggcggaagca aaagcaactc
     9241 cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga attccttctt
     9301 atttcttgga gttaaacgct tctgtcccag cctctttttt cgaataaatg atgtttaatc
     9361 agctgtcctt cggtattcgt tcaagccgct gatttcacaa cctctccact aaacggtgta
     9421 ctaaagctct tttaaggttg ttttccgata taggcaagta tgcctccgtc cacatacaag
     9481 acatggccgt taacgaaatc agatgcgcta gaagccaaga agacagatgg tccagccaaa
     9541 tcgattggct cgccccagcg tgctgctggc gtacgtccaa cgataaattg atcgaatgga
     9601 tgacgttcac cgttttcttg tgtttcgcgt aatggagcag tttgcggtgt agcaatataa
     9661 cctggtccaa ttccattaca ttggatattg tattgaccgt attcagaagc gatgtttttc
     9721 gtcagcattt ttaatccgcc ttttgccgca gcataagcac tgaccgtttc gcgtcctaat
     9781 tcactcatca tggagcagat attgatgatt ttaccgccgc ctttttcgat catatcccga
     9841 ataactgctt ttgaaacgat gaatggggca tttaaatcca cgtcgattac ttggcggaag
     9901 tctgccgcat ccatttcgat catcggtgtc cgtttgatga tccctgcatt gttgactaga
     9961 atatcaactg aacctacatc ttctttgatc tgttgaacca tcgcttgaac cgcttcttca
    10021 tcggtcacat cgcagacata accttttgca tcgatgcctg cttctttata atgcgctata
    10081 ccttcatcaa ctgattcttg attcaagtta ttaaatacga tttttgctcc tgctgtagct
    10141 aaagagcgtg caatctcaaa tccaatacca tagacagcac cggtgactaa tgctactttt
    10201 ccatctaaac gaaacatatc catactaaat tccatcattt tctctccctt atgcttattt
    10261 taattgatcc atttgcacca tatccatgtc tgtataggtg atattttctc cgcacattgc
    10321 ccagatgaat gagtaattgc ttgtacctac acctgaatgg atcgaccagc ttggcgaaat
    10381 aatggcttgt tcattgtcta cgactaaatg ttttgtttca tctggtttgc ccatcatatg
    10441 gaacacacgt gtatcttcat tggcataatc aaaatagaca taagcttcca tacgacgttc
    10501 atgtgtatga caaggcatcg tattccaagc acttcctggt tctaaaatcg tataacccat
    10561 ttataaatga cagctatcgc tgaagcttct actgctgtaa atatccccgt caagatacca
    10621 ccgataatga tgacaatcaa tagaaggcta ggaatcgcct cttttattat ttttccagca
    10681 tgagccatct gtcctttttc gacaacaggg tatttattct ttttagcaac gatgaatgct
    10741 actagcatga ttcctagcgc ccacaaagtg aactgaaccc caaaagttga actatttaat
    10801 ggactgtttc cgatattcta ctggagatag tccatttaac tttagtttta tccttttgtt
    10861 attataccac ctaatatact catttaattt actttgaaat atctcaattg atcggaattt
    10921 ttctcgataa aaaaactctg atttaagcac accaaaaaaa ttttctatta cagaattatc
    10981 taagcaattt ccttttctag acatgctttg aataatgtta ttctctttta attttttttg
    11041 atattgtggc atctgatact gccatccttg atctgaatgt agaatcagtg aaccctcagt
    11101 tccctttttt ttaattgctt gttgaagcat ttcttcaatc agtttatatg ttggactagt
    11161 tgatatacta taactaatta tttctccgtt aaataaatca agtataggag atagatagat
    11221 ttttcttcct tttatcttga attcagtgac gtctgtcacc cattttttat tgggtgtatc
    11281 tactgaaaaa tttcgtttta acacattctt ggcaattttt cctactgttc ctttataaga
    11341 tttatatcgc tttattcgga tttgacaggt aagtcccatt tgggacatta attttctaac
    11401 tgttttatga ttgattgtat atcctttcat ctttaacgct aaagtgactc tacgataacc
    11461 ataagagttt cttgattctt ttacaatcgc tgtaatttct tgctttatct tgctatattt
    11521 atctggctta tctaattttt ttacccagta ataataagtt gacttcgcta attgtgcaat
    11581 tgaaagtaat agattcaatt taaattcttt tttgagctga aggattgttt taaccttgat
    11641 ttcttcttgc tc
//
LOCUS       DACDLI010000075        11626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDLI010000075 DACDLI010000000
VERSION     DACDLI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.075"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..3661
                     /locus_tag="IV353_002210"
     CDS             134..3661
                     /locus_tag="IV353_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9010414.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITVLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3658..7380
                     /gene="addA"
                     /locus_tag="IV353_002211"
     CDS             3658..7380
                     /gene="addA"
                     /locus_tag="IV353_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9010415.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7449..7790
                     /locus_tag="IV353_002212"
     CDS             7449..7790
                     /locus_tag="IV353_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9010416.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8099..9142
                     /gene="pheS"
                     /locus_tag="IV353_002213"
     CDS             8099..9142
                     /gene="pheS"
                     /locus_tag="IV353_002213"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9010417.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9147..11567
                     /locus_tag="IV353_002214"
     CDS             9147..11567
                     /locus_tag="IV353_002214"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9010418.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
BASE COUNT         4011 a         1985 c         2499 g         3131 t
ORIGIN      
        1 ttttttgcgt atacttaaaa aagtagaaat atcagaacct taaaaggata taatttgaag
       61 aatatagaac aaatgttcag tattttttga tataataaag gagcaccgta tcatgtgttg
      121 aaggaggaaa agcatgtctt tacaatttat tcttggaaac gggaaaaaag atcaccgtca
      181 agtcttgatc gacgaagcat ccgcttggct cgaccaggat gcaagtaacc aagtattttt
      241 ccttgttccc aactacagca aattcgaaca ggaacaagaa attttagcgg agatgcgaag
      301 aagaagcgga aaaaaaactt tcagcacaac aaacgttcaa gtatttagtt tttatcgtct
      361 ggcttggtat ttgctgcaac agacaactct tcttacagga aatgaattat cagaatcagg
      421 atctgcaatg atcttgcgac agattttaga aaaaaatgag gaagaattaa caatctttcg
      481 cggagaaatc aataaaagag ggtttattcg tcaattacaa gaactctaca gtgaattgcg
      541 tacaggaaat attcaaccag aagacctgat gcttttattt ggtgattcac ctaatgcaga
      601 agaccagcaa cttaaaatga aagacttgaa gctgattttt tcagcttatg acagcgaatt
      661 gactcaacgt gcactaacaa atgaagatgc actatctata ctagcaaatt atatgcagac
      721 acaagatttc aacaacgtca aatttattgt cagtggtttt tctcgaatca atggtcaaga
      781 gtatcaatta ctgcaggcaa tgatggaaaa ggggcaattg gttgtcgatt tattgctgga
      841 tcgtccttat acagcggatc ttccagaagt acttacgctc tttcatgaga ctggaaagct
      901 ttatttccgt ctttttcaaa gtgcaagaga acggaaagtc cctatattag tggaccgatt
      961 tgcaccagag cagactcgtc cgacagagct tcaactgctg caaacaatgt gggaagagac
     1021 atctagccaa aagaaagcgg cagtacacaa aataggggga caagagcatc tccatttgtg
     1081 ggaagctgaa aatccaactg aagaagtccg acaattagct gttgaaatca gacgactagt
     1141 cagtgaagaa aactaccgtt atcgtgatat ccaagtgctg acaaaaaata tgtctttata
     1201 tggaaatatc cttcggccga tctttcaaga aatggcaatt cctttttata tcgatgaaga
     1261 acaaatgatg gaaaatcatc cgctgatcga atggatcaac agtctgttcg ctcttgaccg
     1321 ctattcttat cggctaagtg atattatgcg ttttttcaaa actgaattat ttctgtcgac
     1381 ggaagcagaa accgaacttg atgaatggaa acaagagcga gatgaattca ggaaaaaact
     1441 ggacatcact gaaaatgtag cacttgctta caattatcaa ggaacttatt ggactcgtga
     1501 aaaagactgg cagtttgtgg cgtatgattt tgaagcagaa cagattgaag acgtacagta
     1561 tttggaagaa cagtcaaata cgattagacg attatttcag aaagctgtac cagtattttt
     1621 gaaaaaaata aagaaaagca aaacaggaca agaggccgca aatgtttttt atcaattttt
     1681 gatccaaagt ggcgtacagc aacaattatt gttttggcgt aatcaggaag tagacagagg
     1741 aaacttagaa gcggcaagaa accatgagca gacatgggga gcgctgattg atctgttgga
     1801 tgaatatgta gtaatttatg gtgaatcggc ttttgactgg tcactgttcc aagaaatcat
     1861 tacaagtggt ctagaaaatc taagttatgg taaaatccct accgcaatcg accaagtgcg
     1921 aatcaatcgt ttggaattag ttcgaccaaa tcaagctgcc gtcacttttg cattagggtt
     1981 gaatgatcaa gtgtttccag accgaaaaga gacaaaagga ttgttgtcca gtgaagaacg
     2041 agaagtatta aatcaggcct tgccagatgg acagttttta ttcgacccat caaaggaaag
     2101 tatctctttt gagcctttcc aagcctatct tgtttttctt tccggaacag atcgattata
     2161 tcttagttat gcacaaagct atgacacaga tggttcgctg aagatgtctc cttatttgcg
     2221 tagaatcaca gaatatttat ctgttcccgt cgagagaaaa gagcatttga cgatcgaaag
     2281 cgaccctaat tgttatgtag ggacttacag aagcggaatc aatacagtca ataggattta
     2341 tcgattagct ttagatgaaa aaaaacctgt accaaagcac tggcaaaagc tcaaagaatt
     2401 agtactgaat tcgtcatggc gaaattttgc tttacagatt tttgaaagtc aggaacattt
     2461 aaatgttcct gtttcgatgc cagaaaaaat ggcggaagag ctttatggaa aagagattta
     2521 tacttcaatt tcacgtatgg agaattttta taattgtgaa tacaaatact tcatgcaatt
     2581 tggtttgaaa ttgaaagaac gaactgttta tggtctaact ccagcagcaa ctggtgattt
     2641 ttatcatgaa tcattggatc gtttcttcaa gctactgttt tcaaaccaat tatccttagt
     2701 gaatatgtcc gatgcgcagc gtagagactt tactgaaaaa gtgctgcagg aagtattcgg
     2761 agaacttcgg tttgagatat tggatagttc tgcaagaatg aactatatcc gctaccagtt
     2821 aggacagacg attcaaaaag tgacatgggc tttgcaaaaa cagagcaaaa atacaggcat
     2881 gcatcctctt cagacagaga ttctatttgg tcagattgct ggagcaaaag gtatcacagt
     2941 tctggaatta ccgttgagta atggtggaaa actccatgtt cgagggaaaa tcgatcgaat
     3001 cgatgtagct tctgaacaag aagatacttg gctaagcgtc gttgattaca aatctagcgg
     3061 acgttcattc gatgtcacag aagcttatta tggcatggct atgcaattat tgacgtatct
     3121 ggacgtagca ttgatggatg cggtacgttt gactggaaag gcggctgtta aaccagcagg
     3181 atcatattat ttacatgttc ataatcctgt attgacggag acagatgatg tagagaagaa
     3241 tacattgaaa aaatttagtt atgatggcgt ttttgtcgat gatcccgctc ttttagaagt
     3301 actagatcat tcactggaag caaaagaaca atcactgatc tatccagtga aaaaaaatgc
     3361 gaaagatgaa taccaaaaag gtagtttcag taaagacaaa tttttcacgg aagaagaact
     3421 gcaactattc atggcgcata atcgggaaaa tatgcggtta gctggagatg cgattacttc
     3481 cggagaaatc aaactaaatc cagcttataa agataaacag cggattgcct gcgaattttg
     3541 tccattcaga agcatctgta cgtttgatgt gatgctgaaa gaaaataatt accaccgtct
     3601 ggagaaaata gataaggaag aagcactgcg tagaatattg aaaaggagtg aagaagagtg
     3661 aatcaaattc ctttaaaacc gcaaaatgaa cgattcacag atgatcagtg gcaagcgatt
     3721 tttgatcaag gagataatct attagtctct gcgtctgccg gatcaggtaa aactacagtg
     3781 cttgttcgaa gagtgattga aaaactaaaa atgggttttg atatcgatga attattgatt
     3841 gtgacattta ctgaagctgc cgcaagagaa atgaaagaaa gaattcaaga ggcattacaa
     3901 gaatccgtca atagcgaaag tgaccctgta cgtagacagc attttactaa gcagttagtt
     3961 ttattgccca cagcgaatat ctcaacctta catgcttttt gcctgacagt tattcgacgt
     4021 tattattact taattgatat tgatccagtc tttcgcatgt taacagatga aacagaaacg
     4081 atcttgatga aggaggatgt gtgggacgag ctgagagaag cactttatgc agaaaatgat
     4141 gaacgatttt tccaattaac aatgaatttt tctaatgatc gttcagatga cggcttgacg
     4201 aatctggtgt tttctcttta tgagtttgcc cgtgccaacc ctgaccccca aaaatggcta
     4261 gaacagttaa gcgataatta tcgtttgcct gaggggctag cgaaatctcg attatatcaa
     4321 aaacaaatcc gtcctttagt tttggcagat atataccaat gtgtacagtt atatgaacag
     4381 atgactcagt tagcacaagg tgaaggatta gaaaaaatgc atgagcaagt agctggagaa
     4441 caacagcaga tcaagaatat ctatgaagcg ttctcgcaag atcggttaga agaagcttat
     4501 gctggtttgg aacaactaac tttttcaact tttaagagca gtcggaaagc agaattgaaa
     4561 gaaatttcca atgaagtcaa gggcatgagg gataaagcaa aaaaactgat tcagcagata
     4621 agtaaaagtt attttcctgt ttccccaagc caaatggaag aattaacgga caaagcacta
     4681 ccgttagtcg aagaaatgac aaaagtcacg caaagcttca tggacggttt cagtatgcgt
     4741 aaacgggaaa aaggtgtgct ggatttcaat gatctggaac atttagcgtt gcagattctc
     4801 acagaaaaaa cgaaagatgt atggctgcca agtgaagctt caaaacatta tcgaaaaaaa
     4861 ttcaaagaag taatggtaga tgaatatcaa gacgtgaacc aattacaaga agccatttta
     4921 tactggctaa gagaacccga tgatacaaaa ggaaatatgt tcatggttgg ggatgtcaaa
     4981 caatccatct attctttccg tttagcagat cctagtttat ttatcgggaa atacgagaat
     5041 ttctcaaaga aagaaggcgg acgtcgaatc gttttggctg agaacttccg ctctagaaaa
     5101 gaagtccttt cttttacgaa tcttattttt gaacaactaa tggatccagc agtgggtcaa
     5161 atcaattatg acgaagcagc caaactgatc caaggatttt cagactttcc agagaatgaa
     5221 cagtttgagc cggaaatcat gatctatgaa aaagaacagg aagaatcaga gattgagatt
     5281 ccgacggatg atatactgga agacaaaaca gaaggcgaac tttttatgac aggactaaaa
     5341 attcgtcaat taattgattc ctcctttatg atttatgata aaaaatcaaa aaaaagtcgt
     5401 ccgatagagt acaaagacat tgttctcttg acacctacaa agaagaacaa cttaacgatt
     5461 ttagagattt tcaaaacatt ggatattcca ttagagatga atgatgctca aaactatttt
     5521 caagcgacag aaattcgcac gatgatctcg ctgctacaat taatcgataa tccctatcaa
     5581 gatattccgc ttgcggctgt attgcgctca ccgatcgtgg gtctgattga accagaatta
     5641 gccagcattc gattagctaa ccgtgcacat acttactatg atgctgtgct tgcttatcaa
     5701 gcatcaaacg aagatgaatt agcagctaag ctggaacatt ttggcaagca actagagcat
     5761 tggcgagaac ttgctcgccg ttcttcgatc actgacttgt tatgggatat ctattatgag
     5821 acgggatatc tagagtatgt ggtcggtctg ccagcagggg cacagagaca agccaatctt
     5881 tatgcattgg ttgatcgtgc caaagcttac gagcaaagtt ctttcagagg attataccag
     5941 ttcgtgcgtt tcattgaaaa aatgcaagaa aaagataaag acttagcaga accagtcatt
     6001 tcaatagaag ataatgctgt tcgcgttatg acgatccatg caagtaaagg cttggaattc
     6061 ccagttgtat ttttgttaga tatgacaaag gagttcaact tacaagactt gcgcaatcgg
     6121 tatgcttttg aagaaaaact tggagcagga attcgctata tggatcctga aacacgagtg
     6181 ttatatgata cgttaccgtt tcaagccatc aaattggcaa agcaaaacaa attgttgtcg
     6241 gaagaaatgc gtaaattgta tgtaggattg actcgggcag aacaaaagct tttcattgtc
     6301 ggttcctata aaaataaaga gcaaatgatc caaacgtggt ctgaagcagc tgatcatgag
     6361 gagttagttt ttgatcctgc gctacgtttg aagggcagaa gcagcttgat gaattggatt
     6421 ggttacggat tgatccgtca tcctgaaatg cagaaatact tagaggaaga aatttcgact
     6481 tctcttcttc aacattcaaa tgcacagttt tctatttcat ggatgaatca gcaatcgatc
     6541 attgaacaaa gacagttgct tgctgaaaaa gaactggtta acttagatca gcagatgaaa
     6601 gaagatgaaa ctttattagc agattcgtta caaaaacgtc tggcatatga atatccttat
     6661 caagcttctt cacaaaccac aagttatcaa tctgtatctg aaatcaaacg attattcgaa
     6721 gatccagatg atacacaaga atcacgtttg actctggaaa gcagtcagaa caaagcagca
     6781 tcccgtcaat ttcgttatac gcaagaacaa ctagcagaac caaaattttt gcaaaaagat
     6841 cggcaagtgt cagcagctgc tgttggaact gctacgcatg ctttgttaca attactgcca
     6901 ttagagatgc caactacaga gagtatccat caaaaattac aggaattggt aaggaaacgg
     6961 ttagtagacg aaaaagtcgc gaaaaaagta gatgtttctt ctattatatg gttctttcaa
     7021 acagaacttg gtcaacagct gattgccaac aaagaaaacg taaaacgaga acaacctttt
     7081 tccatgctgc ttcctgctga cgaagtattt caagactatc caaatcaaga agatgaacta
     7141 ttaatccacg gaatcgtcga tggataccta gaagaaaaag atcatctcaa tatctatgat
     7201 tttaaaacag attttatttt acatccggat gatccagcag aaatagatgc aatcgtccaa
     7261 aaatatcaag gacagctaag actttaccaa caagcaatgt cagaagcttt gaataagcca
     7321 gtagaaaatg ttttcctaat tttgcttcga gtgaaacaaa ttataaacat taacaaataa
     7381 ttataattga gcaaacttga cttatgtttt gtaaggatta tgctatactc tgggaagagg
     7441 tgagtatgat ggagctaaca cagacagaat tcgttttatg ccctaaattt gaaaaaacat
     7501 tctctatttt aggaaaaaaa tggaacggat tgattatcga tgtactactc gaaaacggtc
     7561 cgcaacgctt tgttgagctt gcggcaaaaa tcccgatggt cagtgatcga gtattagtcg
     7621 aacgcttgaa ggaattagaa aaagaaggaa tagtcactcg aaactttgcc tgtagcgaaa
     7681 agagccgttc agaatatctc ttgactgaaa aaggagcaga cttgcaaaaa gcgatgcgtc
     7741 aaatacaagt ttggagcgaa aaatggatca cagaggaaga atgcggttga ttttgtaaaa
     7801 aaaatcttgt aaactatagt ccatacgaat aagtaaagaa cgatgacgaa aaagagtagt
     7861 ttggctaaac cagttcaggg agatctgtcg cagactgaaa gcagatcctg tgtttgaaaa
     7921 atgaagttca ctttccgagt tcgtctgata aaatcagacc ggcaattgtc gttaacgaag
     7981 taagagtgcg gtgtaagtca tttatccgca aattaggatg gtaacgcgaa gacctcgttc
     8041 cttaacagga atgaggtctt tttgttttgt atttggaatt taaaggagga acaagcacat
     8101 gtctctacaa gcacaattag agagcttgaa agaagcaacg ctcgtcaaaa tcacagcggc
     8161 acaagattta aatgaattaa atcaaatacg tgttgaaacg ttaggaaaaa aaggaccgat
     8221 cacagaagta ttacgtggga tgaaagatct aagtccagaa gagcgaccaa aagtcggaag
     8281 tttcgcaaat gagatccgag atcttttgac agaagagatc gagcgaaaaa aacaagtatt
     8341 agaagaagaa gcgttaaatg cagctttggc aaaagaaaca atagacgtga cactacctgg
     8401 tagaaaagta aaacatggta cacgtcatat cgtgacacaa atcatggaag aaattgaaga
     8461 tatttttgtg ggtatgggct atcagatcgt agaaggatat gaagtagaat ctgatcatta
     8521 caatttcgaa cggatgaatc tgccaaaaga tcatccagca agggacatgc aagatacttt
     8581 ctatatttca gacgagatat tgattcggac acatacttca ccagtccaag cacggacaat
     8641 ggaaaaacat gatttctcta agggtgctct acgaatgatc tcgccaggga aagtcttccg
     8701 cagagataca gatgatgcga cccacagtca tcagttccat caaattgaag ggctagttgt
     8761 tgataaaaac atcacaatgg gcgaccttaa aggaacatta gaagtcgtaa tgaaaaaaat
     8821 gtttggggaa gatcgtgaaa ttcgtttgcg tccaagttat ttcccattta cggaaccatc
     8881 agtagaagta gatgtcagct gtttcaaatg tggtggtgcc ggttgtaacg tatgtaaata
     8941 cactggatgg atcgaaattt taggagctgg catggtacac ccgaatgtgt tgaagatgtc
     9001 aggaatcgat ccagaagaat attcaggctt tgctttcggc cttggaccag accgcgtagc
     9061 tatgttgcgg tatggagtga atgatatccg taatttctat caaaatgact tacgtttctt
     9121 aagtcagttc aagggggagt aagaaaatgt tagtttcata taaatggctc aatcgttatg
     9181 tagatttatc gaatgtaaca ccaaaagaat tagcggataa gatgtctgtg acaggaatcg
     9241 aagtagaggg agtaacggtt ccagaagaag gcttgaaaaa aatcgttgtc ggtgaagtca
     9301 aggaatgcgt accacatcca gattctgatc acctttctat ttgtcaagtc gatattggcg
     9361 aagacgaact gtctcaaatc gtttgtggag cgcctaatgt gaaggttgga atcaaagtga
     9421 ttgttgcact tcctaactca cgtatcgctg gtaatgtaaa aatcaaaaaa ggaaaaatgc
     9481 gtggccaagt atcaaatggc atgatttgct cattacaaga aattggctat tcagatagtg
     9541 ttataccgaa agaatacgct gaaggtatct attatatgcc acaagaagct gtaaatggcg
     9601 atccagtttt tccttatttg gatatggatg attcgattat tgaattatct atcacaccga
     9661 atcgtgcgga tgcattgtca atgcgtggcg tagcttatga agttggggca atttatcgtc
     9721 agactcctac attcgatgac gaaaagctgg tagaatcctc acgtcctgct agcgataaaa
     9781 ttcaagtgga agttgaagat aaagaagcag ttcctgctta tcaaatcaga atcattgaag
     9841 gagtaagaat ccagccaagt cctcaatggc tgcaaaatct tttgatgaac gaaggcattc
     9901 gtccaatcaa caatgtagta gatgtgacga actatatttt gcttttattc ggacaaccac
     9961 tacatgcatt tgactacgac aaattgaaat caaaagagat ctttgtccgc cgtgcaaaag
    10021 aaaatgaaac attagtaaca ctagatggag aatctcggga attgacaact gacaatctcg
    10081 tcattactaa cggcaaagaa ccagttgctt tagctggtgt catgggaggg ttagactcag
    10141 agatcacgaa tgaaacaaca acagttgcac tagaatctgc tttatttgat ccaatttcga
    10201 ttcgtcgtac agcaaaacaa tttaatttgc gtagtgaatc ctctgcacgt tttgaaaaag
    10261 gaatcaacca tgcgactgtt ggagaagcca gtgaggttgc tgcagcaatg atcgtttctc
    10321 ttgcgggtgg ggaagttcta caaggcgcgg tcaaaggctc agagatcaaa gcagaagacg
    10381 tatcagtgaa gatttcactt gaacgcatca atcaatattt aggaacatct cttgtcgtta
    10441 aagaagtaaa tgagattttt gaagcacttg gatttagcta tgaagaaaag gagtatgttt
    10501 attccgtagt agtaccacca agacgctggg atatccaaat cgaagcagat attatcgaag
    10561 aagttgctcg tatttatgga tacgaccagt tgccatctac acttccaagt ggagaaacgg
    10621 ttgctggaag cttgtcaaaa gaacaagaaa ctactcgtaa aattcgtaca atctttgaag
    10681 gaagcggatt gagcgaagca atcagttatg cattgacaac agaagaaaaa tcaagacagt
    10741 ttgttataaa tgaaagtaat attacacgtt tggactggcc aatgagcgaa gaacgttctg
    10801 tattacgaat gaatttgatc agcggactat tagatgatat cagttataac actgcccgaa
    10861 aaaatacaga gattgcttta tatgaaattg gtcgggtgtt cttccaagaa aatgatccac
    10921 gtacacatct gccgcaagaa gtaaaacatg cagcaatcgc tatttctggt atttgggaag
    10981 aaaaagattg gcagacgaaa gctcaaaatg tagacttttt caccatcaag ggtcttttag
    11041 aaaacttatt tgaacaacta ggcatcactg aagatgtcca ttatcaaaca gtgacagaaa
    11101 tgaaggaaat gcatcctgga agaactgctg cgatttacct tggcgaaaaa tttattggtt
    11161 ttgtcggtca agtgcatcca acgattacaa aaagttatga tattccagaa acatatgttg
    11221 ctgagtttga tttatctgct atagtggaag ctgctcaaaa agggatcgtt ttcgaagcag
    11281 tgacaaaatt cccagcagtc agccgggaca tcgctttgct tgtaaaagaa actgtgacaa
    11341 atcaggaatt aacagaagtt atccgttctg ctgccggaag atttttaaca gatatccaat
    11401 tatttgatgt ctatcaagga gaaaacattg aaaaaggaca taaatccatg gcgtatagcc
    11461 tgacattcgt caacccagaa gcaacattga cagatgaaga aatcaataaa gcaatggaaa
    11521 aagtcgaaaa agcgttggta gaaaacctag aggcaagtat tcgctaagaa ggaaaatata
    11581 gtaagtaacg atcaagtagc ttttgaagac catacatttg aaaaaa
//
LOCUS       DACDLI010000076        11150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDLI010000076 DACDLI010000000
VERSION     DACDLI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.076"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV353_002215"
     CDS             6..305
                     /locus_tag="IV353_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9010419.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV353_002216"
     CDS             378..1739
                     /locus_tag="IV353_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9010420.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV353_002217"
     CDS             1761..2021
                     /locus_tag="IV353_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010421.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV353_002218"
     CDS             2035..2787
                     /locus_tag="IV353_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9010422.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV353_002219"
     CDS             complement(2881..3486)
                     /locus_tag="IV353_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9010423.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="IV353_002220"
     CDS             complement(3542..4516)
                     /locus_tag="IV353_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9010424.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5673)
                     /locus_tag="IV353_002221"
     CDS             complement(4894..5673)
                     /locus_tag="IV353_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9010425.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPPDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="IV353_002222"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="IV353_002222"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9010426.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="IV353_002223"
     CDS             7164..8561
                     /locus_tag="IV353_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9010427.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="IV353_002224"
     CDS             8642..9082
                     /locus_tag="IV353_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9010428.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="IV353_002225"
     CDS             9229..9555
                     /locus_tag="IV353_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9010429.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="IV353_002226"
     CDS             9556..10305
                     /locus_tag="IV353_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010430.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="IV353_002227"
     CDS             complement(10331..10741)
                     /locus_tag="IV353_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9010431.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3538 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
//
LOCUS       DACDLI010000077        10995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDLI010000077 DACDLI010000000
VERSION     DACDLI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.077"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(116..1126)
                     /gene="trpS"
                     /locus_tag="IV353_002228"
     CDS             complement(116..1126)
                     /gene="trpS"
                     /locus_tag="IV353_002228"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9010432.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            1380..2117
                     /locus_tag="IV353_002229"
     CDS             1380..2117
                     /locus_tag="IV353_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9010433.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            2131..2946
                     /locus_tag="IV353_002230"
     CDS             2131..2946
                     /locus_tag="IV353_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9010434.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            2959..3606
                     /locus_tag="IV353_002231"
     CDS             2959..3606
                     /locus_tag="IV353_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9010435.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            3622..4278
                     /locus_tag="IV353_002232"
     CDS             3622..4278
                     /locus_tag="IV353_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9010436.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            4440..5261
                     /gene="racE"
                     /locus_tag="IV353_002233"
     CDS             4440..5261
                     /gene="racE"
                     /locus_tag="IV353_002233"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9010437.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            5264..6619
                     /gene="rph"
                     /locus_tag="IV353_002234"
     CDS             5264..6619
                     /gene="rph"
                     /locus_tag="IV353_002234"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9010438.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            6620..7138
                     /locus_tag="IV353_002235"
     CDS             6620..7138
                     /locus_tag="IV353_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9010439.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            7245..7745
                     /locus_tag="IV353_002236"
     CDS             7245..7745
                     /locus_tag="IV353_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9010440.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(8025..8945)
                     /locus_tag="IV353_002237"
     CDS             complement(8025..8945)
                     /locus_tag="IV353_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9010441.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            9110..10123
                     /locus_tag="IV353_002238"
     CDS             9110..10123
                     /locus_tag="IV353_002238"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9010442.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(10233..10988)
                     /gene="fabI"
                     /locus_tag="IV353_002239"
     CDS             complement(10233..10988)
                     /gene="fabI"
                     /locus_tag="IV353_002239"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9010443.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         3525 a         1960 c         2319 g         3191 t
ORIGIN      
        1 aaaaaagatg tgacacaact tcgtcacatc tctttttttg attttaaaaa aggaatagca
       61 cagagacgag ataaaaaaac aggcctttac agcctgcttt tttagagtaa atgaattaat
      121 ggaagtaatt gattcccatt gctgatttta cttcagaaag tgtttgtgca gctactttag
      181 cagcttcttc actaccttta cgtaagattt ccataacagc tgaaggatct ttttccaatt
      241 cttctcggcg cgcacggatc ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt
      301 tgatctttac atcaccaagt ccgccgcggc ggtaatgagc tttcatttct tcaattgctt
      361 ctttatctgt tccgaataca tccaagtagg tgaagaccat attcccttct acttgtccag
      421 gatcttgtac atggatgtgg tttggatcag tatacatact catgactttt ttctgtaaga
      481 catcggcagg atcggagata taaataccgt ttcctaacga tttactcatt ttgccatttc
      541 catcgattcc aggtaatcta cccattcctt ttggtgggaa tacgcctttt ggttccacca
      601 agacttcacc atatgtatga ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg
      661 gtttttgatc ctcaccaaca ggtacaagat tcgctttgaa tgcagtgata tctgctgctt
      721 gagaaacagg atagataaag aatcctgtag gcacgctttc accgaatttt ttttgttcga
      781 tttctgtttt gacagtcgga ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat
      841 acatggttaa ttcagctaat tccggaattt gtgattggat aaataaagta gattttgcag
      901 gatctaatcc cacggctaaa tagtctaaag caacttgcaa gacatttgat gaaaccttct
      961 caggattttt agcattatct gtcagtgctt gcatgtcagc aatcatgaca aacagttggt
     1021 tgtcttcatc tgcctgcata gccactcggg tttttagaga accgacgtaa tgccctaaat
     1081 gcagcttccc agttggtcga tctcccgtta aaataatatt tttcaaaaaa gacacctctt
     1141 tacgatttgt aagtctgagc acttaatatc ttaccgtttt tcctgaaaag aaacaacagt
     1201 tcctaaggaa aaaacaagga aagaggataa aataactaat agattagaaa tggaagaaat
     1261 atttatggga gaataaacgt tctaagaaac tttacaaatt actaaactat cagatataat
     1321 gtattttatt atacatcttt aaaagaaatc ttactaaata ataatgggag tgtaattcta
     1381 tggcaatgat taaatttgat cacgtggata aatattatgg caagttccac gcattaaaaa
     1441 atatcaatct cgaattcgag aaaggagaag ttgtcgttgt cattggtcct tctggttcag
     1501 gaaagagcac gatgcttcgc tgtatcaatg gattagagac gatctcttca ggtaagctgc
     1561 tgattaatga tactgacttg catgataaaa agacaaaact gacagaagtc cgaaaaaaca
     1621 tcggcatggt tttccaacat ttcaatttgt acccaaataa aactgttctg gaaaacatca
     1681 aattagcacc aatcaaagtg ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga
     1741 aatttttgaa aactgtcaat atgttagata agaaagattc ttatccttcc atgttatccg
     1801 ggggacaaca gcaacgggtg gcaatcgcca gaggtttagc gatgaatcct gagatgctgc
     1861 tgtttgatga acctacttct gctttggatc cggaaatgat tggtgatgta ttagacgtta
     1921 tgaaaaaatt ggctcatgac ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg
     1981 ccaaagaagt ggcggatcgt gttatcttca tggctgatgg agaagtattg gaagacagca
     2041 gaaatgtgag agactttttc gaaaatccaa atgaagtccg agcacagcaa tttatcagta
     2101 aagttatcaa tcattgatag gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt
     2161 ttccttttag gcattgcttt tcttgcacta gccggatgta aaggaaatgg gttggcaaat
     2221 gaagatattt tagagcgaag caaatctacc aatgaaatca tctggggtgt caagtatgat
     2281 actcgattat ttggaatgat ggatattgag agcagaactg tacaaggatt tgatgtagat
     2341 attgcaaaag caatcactaa aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa
     2401 gtcacttcta aaactcggat tccattattg aaaaacggaa atatcgatgc aattattgca
     2461 acgatgacga tcacagatga acgaaaaaaa caggttgact tttcagatgt ctatttcgat
     2521 gctggccaag ctttgttggt aaagaaagga agccaaatca aaagtgtaga tgatttgaat
     2581 gcttctacaa cggttctagc agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat
     2641 gcaccagatg caaaaatttt agaattagaa aactatgcag aagcatttac tgctttacaa
     2701 tctggtcagg gagacgcgat gactacggat aatgcgattt tgttagggat tgcagatgaa
     2761 aatccagagt atgagttagt tggtggaacg tttactaatg agccttacgg gattgcgatc
     2821 aacaaagggc aagaaaactt cttgaaagct gttaaccaag cgctagagga aatgcatgca
     2881 gatggcacat acgataaaat ctatcaaaaa tggtttccaa atgaaactga aggaaaagta
     2941 gagtaagggg gagaggcaat gagcgaattg atacaaactt atggaccaat ctttgtcgac
     3001 gggttcaaga tcacattgtt ttcgagtgtc ttagcacttg tgtttagttt gattatcgga
     3061 acgttaatgg caatcttcca attatcaaag aataaagtag tgagcgcctt agcaaaggca
     3121 tatgtagaat tttttagaaa cattccatta ttaatcatcg tgatgttttt ttatgttgtc
     3181 gtaccgattt attggtttaa tctagatgga ttccaagcag gtacgattgg attaacgatc
     3241 tatacatctg cttttattgc tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt
     3301 caaacagaag caggactatc ttctggtttt acctattcag aaatcatgcg ttatattgtt
     3361 ttaccgcaag cattcaaaat cgttatcccg ccattaggca accaatttat caacctcgta
     3421 aaaaactcat ctattttagc aatcgtagct ggtttagatt tgatgtatca aggagacttg
     3481 atcgctagtg aaacattcaa taccttcgat acgtatatca tcgttgggtt attttactta
     3541 gttatcacgc taccgttatc ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt
     3601 tcatagaagg aggggaaata catggatttt agcggcgctt tttcatggat gaatatccgt
     3661 tttttattgg aaggattaaa ggttactgtt gaagtttcat tgttttcgat catttttagt
     3721 ttcttgatcg gtggtctgac aggtgtgatt cgttttgcga atatcccttt cttttccaaa
     3781 atccttggtg tagtgattga tattatacgg aatcttccat tattattgat cattttcttc
     3841 acttactttg ccttacctca aatcggcatt cagatgaaca tcttctggtc cgctgtcgca
     3901 gcattaacga tttttgagtc agctatgttg tcggaaattt tccgagctgg actcaatgct
     3961 gttccaaaag gacagatgga agcaggatta tcaacgggac tgacttatgt agaatcgatg
     4021 cgaacgatcg tgttaccgca agcatttaaa tcaatgatcc cggcaattgt gagccaattg
     4081 atttcactga tcaaagatac ttcattagca gtaatcattt ctttacctga actttctcat
     4141 caggcacgta ttatttatgg acagaacacc aattatgtgc ttccgatgtt catcatcatg
     4201 acacttatgt acttcgtggt atgttacgcg ctttctctgc tttccggtta cttggaaaag
     4261 agacggtaca gttattaata gatgattctt ttagcggttg tggcaaacta aagtcacaac
     4321 cgcttttcct ctttccattc gaaaaaaaga gatttcgatt gttttaagca agtaagagca
     4381 cttgaaaatt cacgctaaaa agtacacaat agggaggata gaatttattg aaaaaaagga
     4441 tgatacgatt gacagataat cgccctatcg gatttattga ttcaggtgtc ggcggcttga
     4501 ctgtagtaaa agaagccctg aaacaattac cgaatgaaaa tattttattt gtaggagaca
     4561 cagcacgctg cccatatggc cctagacccg cggaacaggt aatccagtat acttgggaaa
     4621 tgacggatta tctggtggag caaggaatca agatgctggt gatcgcctgc aataccgcaa
     4681 ctgcggtggc tttagaagaa atcaaagctg ctctttctat tccagtcatc ggtgtgatcc
     4741 ttcccggtac tagagcggca gtaaaaaaaa cacaaaataa acaagttggc attatcggta
     4801 cgattggtac ggtaaaaagt caagcttatg aaaaagcact gaaagagaaa gtaccagaat
     4861 tgactgtgac aagtcttgct tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc
     4921 attcatcggt ggcgaaaaaa attgtggcag aaacattagc tcctttaacc actaaaaaaa
     4981 tcgatacatt gattttggga tgcacccatt atccattatt acgccccatc attcaaaatg
     5041 taatgggaga aaatgttcaa ctgatcgatt ctggagcaga aacagtaggt gaagtatcta
     5101 tgctgttaga ttatttcaat ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt
     5161 tttatacaac tggctctgcc aaacttttcg aggaaatagc tgaagactgg cttggaatcg
     5221 gacacttaaa tgtagaacat atcgaattgg gaggaaaata aggatgcgtc atgacgggag
     5281 aaaagcccaa gagcttcgca aactggagct tcaaacaaat gttttcaaat atccagaagg
     5341 atcggtagtg atccgttttg gtgatacaac agttatttgt tctgcaacaa tagaaaattc
     5401 tgttccccct tttttgagag aaacaggaac aggatgggtg acagcagaat atagtatgct
     5461 gcctcgagca acgagtacga gaaatcgtag agaaagcagc aaagggaaac tttctggtag
     5521 aacaatggaa atccagcgtc ttattggtcg ttccttgcgt gcagtagtag acttggaaaa
     5581 attaggagaa cgtagtatca tcgtagactg cgatgtgatc caagcggatg gcggtactag
     5641 aacagcaagt atcactgggg catttgtggc attgaaatta gccattgaaa aacttttaag
     5701 agaaaaagaa ttgtcagaag atccaataaa agaacatctt gctgcagtta gcgtaggaat
     5761 cttaccagat gggacttgcg tcacagattt ggattaccaa gaagattccg cagcacttgt
     5821 agacatgaat cttgtaatga cagaatctgg aaaattcgtg gagattcaag gaactggaga
     5881 agaagcaacg tttgatggtg aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat
     5941 tgaagatttg atcaaagagc aaaaacatgc tttgctaaca gagtttgctc aaaatgatga
     6001 gcggatagaa gaaacaaaaa caatcatcat tgcgacaaga aatccaggaa aagcagaaga
     6061 gtttcgaaat atgttcaaag aagcgggata tcatgtaaaa acattacttg actatcctga
     6121 attgccagat gtggaagaaa ctggcagcac atttgaagaa aatgcccgat taaaagcaga
     6181 aacgattgca caattgcttg atcagccagt cttagcagat gattcaggtt tgaaagtgga
     6241 tgctttaggc ggtatgccag gaatctattc tgcacgtttt gcaggggaac aaaaaagtga
     6301 tgcggggaat aacgccaaat tattatatga attgacagat gtgccggatg aaaaaagaac
     6361 agcacaattc cattgtacgt tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga
     6421 agcggaatgg cccggtcgag tagcccgtat accatctggc gaaaatggct ttgggtatga
     6481 tcctttattt atcccagaag gtaagaaaca aacagcagca gaattatcga gcgaagaaaa
     6541 aaacaagatc agtcatcggg ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg
     6601 gctggaagga gagagataga tgcggtattt agtagtaagt gataatcatg gagacagaga
     6661 tatcctcgtt gacttagtag atcgttatcg ttctgaagtg gatatctttt tccattgtgg
     6721 tgattcggaa ttagaagctt ctgacccatt atgggataca ttccaagtgg ttcaaggtaa
     6781 ttgtgattat ggaccaggat tcgaacagaa aaaagtgatc caaactggtc aggatacagt
     6841 attcatgact cacggtcatt tatcgaatgt ccgatttggt ttgacacagt tagcaatcga
     6901 agctcgagct gcaactgccg atatggtgtt ttttggtcat acccatcaaa taggctgtga
     6961 gatggatcag aaaatcctgt ttttgaatcc tggtagcatt tctcagccta gaggcccgat
     7021 ccaaatccca tcatatgctc ttatcgaaag tttagatgac caaatttctg ttcaatacta
     7081 taaccgtgcc catcaggcag ttgaagagtt ggccttttct ttttcgagag agcgctgaat
     7141 aaaaaaactc taatttttaa taaaaataag ctttatttta tgaattttgc atccggtttc
     7201 ggtaaaatag tagtgttgaa tgaaatagaa atggaggcca tcagatgatt ggacctatag
     7261 taagagaaat gctgctggaa aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa
     7321 atgttatgta tcatcatcca ttgtcccatg gtttacttgt gttatcgaag gtcggttatt
     7381 ccaaaattcc tgtattagga aaagatgatc actttgtagg actagtaagt ttatcagatg
     7441 tggtcaataa aatgattgac ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg
     7501 ttgctgatgt gacagaaaca gatgttccga caattgatga aaattgggaa ctagaagaag
     7561 tactccattt gttagtcgat tcgccttttt taccggttgt cacagaagat cggattttca
     7621 aaggaatcat tacaagaaaa gagctgctta aagccgtaaa cttcatggtg catgaattag
     7681 aacgtcgtaa tatattgtct ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga
     7741 gctaaagaac gagaaactaa aagataatca aaaaataagt agaaaaaaca gcgtaccagt
     7801 tttgacttta tcttgaaaaa gaggctggga caaaagcgtt taactccaag aaataagaag
     7861 gaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggagttg
     7921 cttctgcttc cgccgtttat acgtgtttag agcgtgggac aaaagtgttc tttacttttg
     7981 tcccacgctc tttttagtga gattcgttgc atcgtttgtc caaattattt tccgacagca
     8041 attggactgt tcggaatcgt gaaaccagaa gctgtcaatt ctttgacata ggagctcaag
     8101 aactcttctt ttaaggcata ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca
     8161 aaattgctgt ttccaagatc cactaatcca aagatagaag gttcagtttg gagctcttct
     8221 tggtattttt cagctaatgt acgatttact cgatcaatga tctcatagat tttgtcatat
     8281 ccttcttcag gaacaatccg aatatctaaa agtacttgca tatttgcacg agagagattg
     8341 ctgatcgtgg tgatgtttcg attcgggatg aaatggacag taccatccgt tgctttcagc
     8401 tgaagggtac ggataccaac agatacgacg gttccttcaa tcgttaagtt tgctagcttg
     8461 atgtagtccc caacatccat ctgttgttcc atgataataa aaaatccggt aataacatca
     8521 ctcatgaacc cttgggcacc aagaccgatt gccacacctg caattccggc accagccaaa
     8581 agtgatccga tcggcacacc gatagcagat aaaattgcat agataaaaaa gaaaccaagg
     8641 gtataatgaa aaatagtatt taaaagacta tgtaaagtct ttaagcggct ctcgtttaga
     8701 atggcttttt tagaatactg attaaaagac ttatcaatca gatattttcc cacacggtga
     8761 agaatccaaa aaaggacgat caaaaaaatc agatataaac ttttttggat aaaggcagcc
     8821 aaaatggcgt cccaatcaat cccgtcccaa aaccgctgaa acgcatttac tttgctgaca
     8881 gcttgatctg tcaatgtctc gccagaagag tctgtcgtac cggcagttgc taataatgaa
     8941 aacataaaat cctcttttct aaaatttgat tctttctgta ttgtaccaag aagttttcaa
     9001 aaaaggaaat agcaaaaata actataatga aggaaaaaag ctgaaaataa aatgacaaat
     9061 aatatggata atgataaaat gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga
     9121 ttggaacaat ctaggttttt cttatatcaa aactccttgg cgttttattg ccaaatggaa
     9181 agacggacaa tgggaagaag gagaattgac agaagataat tacctgacga ttcatgaagg
     9241 ctctccagcg cttcattatg gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa
     9301 agacggcaag gtcaatctct ttcgacctga acaaaatagt cgacggatga atcaaagtgc
     9361 tagacgattg ctgatgccaa tggtccctga aaagatgttc gttgatgctg tcaagcaagt
     9421 ggtaaaagca aatgaagatt ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg
     9481 ccctttactg attggggtcg gtgaaaacat tggtgtccat ccagctccag aatatttatt
     9541 tgtcgtcttc tgtacgccag taggagcgta tttcaaaggt ggactaaaac caacgaattt
     9601 tattgtatct gattacgatc gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg
     9661 aaattacgca gcgagtttac ttccaggaac agaagcaaaa gaactggatt actcagattg
     9721 tgtttatctt gatccagcca cacatacgaa aatcgaagaa gtaggtgcag caaatttctt
     9781 tggaattaca aaagacggaa aattcatcac tccgcaatcg ccctctatct taccaagtat
     9841 cacgaaatat tccttgttgc agttagctga agaacgacta ggactgcaag caattgaagg
     9901 agatattttc attgatgatc tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc
     9961 tgttatttct ccaatcggtg ggatttatca caataacaat cttcatgtat tttatagtga
    10021 aacggaagta ggaccagtaa caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg
    10081 agacatagaa gcaccagaag gctggattga agtcgtggaa taatttcaaa gtaatgagtt
    10141 gaaaaaaacg taaaaagaaa aaatcaggaa gagactttaa aaagtttttt cctgattttt
    10201 atatgtttta ctttaaagaa tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg
    10261 tagataatat caccgatgat tccgctggaa agtgggctga ttaagaaagc acaagcatta
    10321 ccgacttctt cgatcgtcac accttgctga tcaggcgtac gagattcaga taactggatc
    10381 agtttttgat agtctttaac acctgtgaca gctaatgttt tgattgcccc agctgagatt
    10441 ccattgaccc gaattccttt aggcgagaac tcagctgcca aataacggat agcagcttca
    10501 agagaagctt tcgcaatacc catcatgtta taattcggga ttgcgcgttc tgaacctaga
    10561 taagtcatgc taacgatccc gctgtttggt gcaagaaacg gttgtgcgta ttttgataca
    10621 gcaattaaag agtagctgct gatatcttga gctaattgat agccgtctct ggaaatatca
    10681 gacacatttc cggacaattc ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg
    10741 atctccccag catattcacc gatggcattc attgcttttt cgatgctttc gtcgctagaa
    10801 acatcacatt ctacagtgaa tgcttcttcg ccgaccaatt tgactaatga ttttttcatt
    10861 cgttcatttt ggtaagtata gataatttct gccccttgtt tcaacattgc ttcagcacaa
    10921 ccccaagcaa tgcttttttt gttagcgacg cccataatga cgattttttt tccttctaga
    10981 aatgacataa tgact
//
LOCUS       DACDLI010000078        10935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDLI010000078 DACDLI010000000
VERSION     DACDLI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.078"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..154
                     /locus_tag="IV353_002240"
     CDS             <1..154
                     /locus_tag="IV353_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9010444.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /locus_tag="IV353_002241"
                     /pseudo
     CDS             complement(235..1218)
                     /locus_tag="IV353_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="IV353_002242"
     CDS             complement(1613..2455)
                     /locus_tag="IV353_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9010445.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="IV353_002243"
     CDS             2780..3196
                     /locus_tag="IV353_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9010446.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="IV353_002244"
     CDS             3193..3663
                     /locus_tag="IV353_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9010447.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="IV353_002245"
     CDS             3677..4450
                     /locus_tag="IV353_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9010448.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="IV353_002246"
     CDS             4464..5276
                     /locus_tag="IV353_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9010449.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="IV353_002247"
     CDS             5330..6295
                     /locus_tag="IV353_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9010450.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6411..9134
                     /locus_tag="IV353_002248"
     CDS             6411..9134
                     /locus_tag="IV353_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9010451.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGSRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9147..9779
                     /locus_tag="IV353_002249"
     CDS             9147..9779
                     /locus_tag="IV353_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9010452.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9984..10211
                     /locus_tag="IV353_002250"
     CDS             9984..10211
                     /locus_tag="IV353_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9010453.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10280..10546
                     /locus_tag="IV353_002251"
     CDS             10280..10546
                     /locus_tag="IV353_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9010454.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10536..10805
                     /locus_tag="IV353_002252"
     CDS             10536..10805
                     /locus_tag="IV353_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9010455.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3622 a         1741 c         2140 g         3432 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata ttgtgaaagc aggtggcaag atgcagcgat
     6421 taaatgacta tctttatcaa ctaacattgc ttttgaaatc tacgccttca gataattatt
     6481 cagctcgcag tatttcgcaa gctctacagt tgaatagaag tacaatcagt agctacttaa
     6541 atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag ttacccggtt ttatttttac
     6601 atagaacagc tcttgaagaa ctacatatca cactgaataa ctccgaaata gaatcaattg
     6661 aaagcttgtt aacaatttct caaaaacccg cattagatca agtaattggc tctaaaggaa
     6721 gtttgaaaga agcaattgat caaattaaaa cggcagtctt atatcctgga aaagggttgc
     6781 ctttattatt gatcggcgct agtggatcag ggaaaacctt tctagcgaat aaaatatatg
     6841 aatatgcagt agaagaaaag gtcattcaca catcggcacc tttcatagat tataattgtg
     6901 cacaatatgt gagcaatcca gagttactat cctctgcttt atttggctac acgaaaggag
     6961 catttaccgg agcaagccag gagcatactg gtttgctaga aaaagccgat ggaggagtat
     7021 tgtttttaga tgaagtgcat cgcttatctg aagaagggca agagaaatta tttacattta
     7081 tggatacggg tgaattctct cctatggggg ataatagtat tcggaaaaaa gccgatgttc
     7141 gattagtctt tgcaacaaca gaaaatatat atacgacttt cttaccaact tttcttagga
     7201 gattgccagt tattgttaat ctacctagat ttcaacaacg tccgtcgttt gaacgtctgt
     7261 cattgattga tgaatttttt gtttctgaga gccaaatttt agctaaggaa ctatctgttt
     7321 ctgatgcatt gattcatttc ttaatgaata gtgattttga aggtaacgtg gggaatatta
     7381 aaaatattat caagtatgct tgcggaaacg catatatcca ccagaagagt cagcaaacaa
     7441 ttcaagttaa attattggat atgcctttag agtacagtta taaattcaag gagcaactaa
     7501 ataaacccag aaagaaaaga gcagatcgaa tctactatcc agaagaaaca cgacaagtgc
     7561 aactagaaag aaacaatcag ctttttgttc aattttttga ccaattactt actggattct
     7621 ctgaagtgat tgaaaataac aaggatatta aactattttt agaagaaatg gttaccaagg
     7681 taacacaagt aatggatacg cttatttttc aagaggatta cgaaaaagag cagtctttat
     7741 acgccattct aaactatcat attcgtcaaa gtattgattt tatgcgagaa agctacgggt
     7801 ttgaacaaga tggtagccgg gtagtcgctt tggccaacta tctatatgtc aaggataatc
     7861 aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa agatcagctt aacgaatttt
     7921 ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa aatcttaagc cttttatctg
     7981 agtgtttaga tcaacctgtg tatgaagagg acattttctt tattacgcta tatttttata
     8041 gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat tgtgctagct catggttatt
     8101 ctacagctag tagtatggcg aatgttgcca accgtatgct gaataagaac gtttttcaag
     8161 cttatgatat gcctttagat attacattag agaaaattga agcaaaattg attcaattcc
     8221 ttggagatta tcgaacagat tcaggcttga ttttattggt agatatgggt tcactaaacc
     8281 aactaggcgg tttattagtg aatcatctaa atggaccact gttaatcatt gatcatatca
     8341 atacaccgtt attattggaa gtcggccaac aagtattaaa caataaaagt atcaccgaaa
     8401 taggagcaga aattgaaaca agtcttactt tcaaaaagca gttactactt cccaagaaaa
     8461 agaaaaagaa agccatcatt acttgttgtt atacagggat tggtagtgcg acccagattc
     8521 aggagatttt aacgaaatgc ttgaaagatg cagttagaga attagtaatt attccgtatg
     8581 attacaaaaa gctgttagaa aacaagaatt ttgagactcc ttttcaatta tacgatgtca
     8641 tcatgattgt aggtacagaa gatcccaaaa ttaatcagat tccatatatt ggattagatc
     8701 gcctgattaa tgggggaagc attggcgaat ttgttcatta cttggaaaaa gagctgacta
     8761 ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt ttcaattagt aaaattgtcg
     8821 aaaatttaac cattttggat ccagataagg tgctatcttt tgtacaaaaa gcaattaaag
     8881 aattatcgaa gttattaaat tttgattttt ctaataatca gctgtttttg ttgtaccttc
     8941 actgtagttg tatgattgaa cgaattgtac gtaaagaaag tgtggatgag caagaggaca
     9001 ttgaagaata tatgcaagca aatggagaaa taatggataa aattcatagc gctttttttg
     9061 agattgaaag ggaatacaca attgaaatac cagatttaga gttgcgatta ttgagcgata
     9121 ttgttcaagg ataggagtga attcgaatga caatgggagt aatttttgat atggatgggg
     9181 tactcataga tagcgagaca ttttattttg agagaagaat gaacttcttt caagaactgg
     9241 agatagatcc aggatcaaac cagattgatg attatgtggg gaaaacagaa gctgcaatat
     9301 gggaaacatt agttcctgag gacaaatcat taagagagga actttatccg aaatatgtag
     9361 attatcgaaa aacgcatcca attgatttcg ctaaagcttt acgaaaagaa gtgaaaccgt
     9421 tacttgactt cctcaagaag cgggatatac cattggcact ggcatcttct tctccagctg
     9481 tcgagataga gagtatgctc attcaatgtg aacttactga atatttttcc tttgttataa
     9541 gtggtgagtc attaaaacaa agcaaaccac atcccgaaat atatttaaaa gcgaaacagc
     9601 aattagactg tgacttcttt attgcagtag aggattcaac gattggtatt aaatcagcaa
     9661 aggcagcaaa tatctatacg gcggcactaa aacaaccaat gcaaatcgat cagagtgaag
     9721 cagacataca aattgaaagt ctacaagaac tggaacaaat tgttttagaa aaaggttagt
     9781 gaattaatgg aaataaggct atatatctgt gctagctatt ttgactaacc aaaaaaactt
     9841 gctataattc aggtgtagta aaaggtgctg gtaacacctt ttacgaaaat gcttcggcag
     9901 tgataatggg attggctttt taagtttttt attttgttcg caatccctaa ggtcatgtga
     9961 atgatcctag ggattgctag aatattttgc aacagaacca aaatacgaat agttatcaag
    10021 cagccatatt gactgcggtc aatttaggag gggatactga tactactgct gccgtaactg
    10081 gtgctcttgc gggtattctt tacgggggtg aagtgatccc tgaaaattgg agaaaatcgc
    10141 ttcaaaatga ccagcttctt aaaacgatca gtcagagatt ttgcgatacc gtgctatctc
    10201 aaagaatttg aataatggat atgatgcaaa tcttgtttgt actaacgttg tattgacaaa
    10261 aaggatttta tttagtacaa tgttagtaca aatgatgaac ggaggtagaa tcatgtctca
    10321 atcaaccatt acatttagga ttcccgacga tgaaaaagaa ctggtattag aatatgcaaa
    10381 agcacacaat tcttcattga ccgaacttta tcgaaatgct gtactggata aaatcgaaga
    10441 tgaaatcgat ctacggactt tacggagtgc aattgaagtt tccaaagaga aaaaagagag
    10501 tggtatttct caagaggaaa tggagaaact gctaaatgaa ttataaagtc atctacatgc
    10561 cgcaagccca gaagcaatta aaaaaaatgg acagaaacca agctaaaata attgtcgcct
    10621 ggatcagaaa gaatttagaa aacacgactg acccgcgtca gcacggaaaa ggtttgactg
    10681 gaaataagag tggggaatgg cgctatcgaa ttggaaacta tcgaatattg gctaatattt
    10741 gtgattctga aattgaaatc cagatattct caattggaca tagaagtaca atatataaaa
    10801 gataaccgtt tttggaacga agtagagttt cgaaagtcaa tgtataatct tttgaatttt
    10861 gcataatacc acaggtagaa actacccgat actatcggtt ggtttctgct tttttatttg
    10921 gtcttgaaga agaaa
//
LOCUS       DACDLI010000079        10216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDLI010000079 DACDLI010000000
VERSION     DACDLI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.079"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..318
                     /locus_tag="IV353_002253"
     CDS             169..318
                     /locus_tag="IV353_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9010456.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            329..1843
                     /gene="dltA"
                     /locus_tag="IV353_002254"
     CDS             329..1843
                     /gene="dltA"
                     /locus_tag="IV353_002254"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9010457.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1840..3045
                     /gene="dltB"
                     /locus_tag="IV353_002255"
     CDS             1840..3045
                     /gene="dltB"
                     /locus_tag="IV353_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9010458.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3090..3323
                     /gene="dltC"
                     /locus_tag="IV353_002256"
     CDS             3090..3323
                     /gene="dltC"
                     /locus_tag="IV353_002256"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9010459.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3326..4594
                     /gene="dltD"
                     /locus_tag="IV353_002257"
     CDS             3326..4594
                     /gene="dltD"
                     /locus_tag="IV353_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9010460.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4735..5817
                     /locus_tag="IV353_002258"
     CDS             4735..5817
                     /locus_tag="IV353_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9010461.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5817..6620
                     /locus_tag="IV353_002259"
     CDS             5817..6620
                     /locus_tag="IV353_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9010462.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6690..7430
                     /locus_tag="IV353_002260"
     CDS             6690..7430
                     /locus_tag="IV353_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9010463.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7441..8445
                     /locus_tag="IV353_002261"
     CDS             7441..8445
                     /locus_tag="IV353_002261"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9010464.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8490..10187)
                     /locus_tag="IV353_002262"
     CDS             complement(8490..10187)
                     /locus_tag="IV353_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010465.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3274 a         1693 c         2078 g         3171 t
ORIGIN      
        1 aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc
       61 tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt
      121 gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg
      181 cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt
      241 gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt
      301 atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga
      361 tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt
      421 tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg
      481 cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg
      541 ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat
      601 cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga
      661 agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc
      721 agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac
      781 ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt
      841 tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc
      901 gccattttct tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat
      961 gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc
     1021 agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga
     1081 acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga
     1141 attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa
     1201 tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt
     1261 actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat
     1321 tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc
     1381 aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca
     1441 aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt
     1501 attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg
     1561 ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa
     1621 ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa
     1681 acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga
     1741 ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa
     1801 aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc
     1861 ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta
     1921 tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt
     1981 ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt
     2041 ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca
     2101 ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact
     2161 cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc
     2221 tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca
     2281 tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc
     2341 tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt
     2401 ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat
     2461 tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt
     2521 tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct
     2581 ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat
     2641 ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg
     2701 ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa
     2761 aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta
     2821 atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt
     2881 ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct
     2941 tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc
     3001 ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat
     3061 taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag
     3121 aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt
     3181 tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc
     3241 aagtccctgt atctgaattc gagcgagagg attggagcac accgaataaa atcattcaac
     3301 aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt
     3361 tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc
     3421 taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga
     3481 tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga
     3541 attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta
     3601 tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg
     3661 ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt
     3721 cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata
     3781 cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt
     3841 attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa
     3901 aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct
     3961 gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga
     4021 tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa
     4081 tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac
     4141 gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg
     4201 tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa
     4261 cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc
     4321 tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt
     4381 caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat
     4441 ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga
     4501 aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca
     4561 acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt
     4621 gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa
     4681 aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat
     4741 acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa
     4801 gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat
     4861 gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt
     4921 tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg
     4981 gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc
     5041 aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta
     5101 acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca
     5161 tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat
     5221 attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt
     5281 atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat
     5341 gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt
     5401 acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc aggagacact
     5461 ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt
     5521 tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag
     5581 gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa
     5641 atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg
     5701 cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca
     5761 gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga
     5821 ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat
     5881 ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact
     5941 tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc
     6001 tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag
     6061 ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt
     6121 ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt
     6181 taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca
     6241 gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt
     6301 tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat
     6361 tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga
     6421 aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt
     6481 ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg
     6541 tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc
     6601 cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg
     6661 taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag
     6721 cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt
     6781 tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg
     6841 atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat
     6901 tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg
     6961 gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac
     7021 atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa
     7081 caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat
     7141 tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg
     7201 atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca
     7261 cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac
     7321 acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca
     7381 aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac
     7441 atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat
     7501 ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca
     7561 atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa
     7621 aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga
     7681 aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa
     7741 gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa
     7801 ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc
     7861 aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc
     7921 aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct
     7981 cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa
     8041 tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg
     8101 tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga
     8161 aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt
     8221 tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa
     8281 atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga
     8341 cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat
     8401 tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg
     8461 gaatccacat atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt
     8521 tgaacagtga tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga
     8581 tccgctacgt ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg
     8641 atgcctcgac taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa
     8701 tgttcctctt gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca
     8761 tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg
     8821 ttgataggaa gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca
     8881 gctaacttct ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac
     8941 agcatttcga aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt
     9001 tggtccgtat gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt
     9061 gtatttactt cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt
     9121 ttgattttta agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt
     9181 agattgtagc tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc
     9241 gttgtttgat agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc
     9301 aagacgatcc aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg
     9361 catgcaagtg aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat
     9421 tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt
     9481 tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag
     9541 cagatgatga aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat
     9601 ggttcaaaaa gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa
     9661 gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag
     9721 atcgtataga aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc
     9781 ggcactgatt gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc
     9841 ggtggcagtc cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg
     9901 atataatttg ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga
     9961 taatctttcc gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt
    10021 cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat
    10081 gtttcgtctt tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca
    10141 ggatcatctt ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc
    10201 gcaggagaaa aaataa
//
LOCUS       DACDLI010000080         9979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDLI010000080 DACDLI010000000
VERSION     DACDLI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.080"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..172)
                     /locus_tag="IV353_002263"
     CDS             complement(<1..172)
                     /locus_tag="IV353_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9010466.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRY"
     gene            complement(239..919)
                     /locus_tag="IV353_002264"
                     /pseudo
     CDS             complement(239..919)
                     /locus_tag="IV353_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1340..1882)
                     /locus_tag="IV353_002265"
     CDS             complement(1340..1882)
                     /locus_tag="IV353_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9010467.1"
                     /translation="MINVSEIKTNKPQKYETLLQEKVYAALESLDISYERVETDEVIT
                     MEDCEQVNLKLDMHMVKTLFLCNRQKTNYYLFITSGDKPFKSKDFSKALGISRVSFAS
                     KEDMTSMLQTKIGAATVFSTLIDTKNKIKIIFDKDVTDDRWYGCSDGTTTGYLKISTN
                     EIIAKLLPYTKHQMEIVKIS"
     gene            2004..2870
                     /locus_tag="IV353_002266"
     CDS             2004..2870
                     /locus_tag="IV353_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9010468.1"
                     /translation="MDMNIQKYIAFLKTVELKSFTKAAEALNYSQSGISRMINDLEDE
                     WGVTLLERNRTGLRLTSDGINIYPYVKNLFDEYESMQRKLEELNGIKFGVIRIGTFSS
                     VATHWLPQLILNFQNKYPNIKFELLLGDYDEIEHWILTGRVDFGFLRLPTLPKLSTQF
                     IHQDDFKVIVPKNHFLEKKDSVSLNDLSGLPFILLDKNGNSNITTLFETMDFSPDINF
                     TTWDDYAIMAMVEKGLGVSILPELILQRVQYDLTILDLDVPLYRKIGIAYKEEKNLSL
                     ASKKFLDLLFFE"
     gene            complement(3892..4035)
                     /locus_tag="IV353_002267"
     CDS             complement(3892..4035)
                     /locus_tag="IV353_002267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010469.1"
                     /translation="MKKYNRSYGEFEEKATRKEFMTVLQWVAEESNRKQRKIAGLDKE
                     EEQ"
     gene            complement(4320..5345)
                     /locus_tag="IV353_002268"
     CDS             complement(4320..5345)
                     /locus_tag="IV353_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase"
                     /protein_id="HAP9010470.1"
                     /translation="MKTIAVVGGHGQVGKGNVRELTNHGYFVVLMGRNLKKMEVFAQE
                     FLPILEQSEVDLNKFVIPEKLADLDMVIVCLDQTDTQFVEQCQKLGIDYLDISANSDF
                     LKKVNLLPIPEKSKRVIGLGLAPGVTNLAAAAYTKKEPSAQMIVIDVLLGIGDRHGEA
                     AVRWTFEQLNRTYIHPKWAQPIENFVYSRKTDFGPPFGKRRSANFGFADQHLLFSADP
                     SRNYVTFLGLDQNWLTALLRTIKKVGLARLFKNPRVIKSPVWFMKRGLVGDRSFVVKV
                     SNGQVKDPEKIVLFGEEEAKMTAEIAVFVANKILIETLPNGHYHLTELTTMSEILSQL
                     PRLSATE"
     gene            6004..6363
                     /locus_tag="IV353_002269"
     CDS             6004..6363
                     /locus_tag="IV353_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9010471.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
     gene            complement(6436..7254)
                     /locus_tag="IV353_002270"
                     /pseudo
     CDS             complement(6436..7254)
                     /locus_tag="IV353_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(7668..8099)
                     /locus_tag="IV353_002271"
     CDS             complement(7668..8099)
                     /locus_tag="IV353_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9010472.1"
                     /translation="MLNKRIANKNDYKLVLEIWEKSVKQTHDFLAEADFNFYKAIIPE
                     NLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFIGKRYGSQILIELIETEDIKRI
                     DVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITHLVKK"
     gene            8200..8499
                     /locus_tag="IV353_002272"
     CDS             8200..8499
                     /locus_tag="IV353_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010473.1"
                     /translation="MTKIYFMLTSNSGVGSTTIFATRARRNIDDLLAFFSQYYDVSAN
                     YTKDKDMIDLLVLPEPFGPFNNERNLPVIQIPTILFLERDFEKIKTHIDAYLAKK"
     gene            complement(8544..9194)
                     /locus_tag="IV353_002273"
     CDS             complement(8544..9194)
                     /locus_tag="IV353_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HAP9010474.1"
                     /translation="MPKVAVMAGTPIDTRMGADLLNEISCELLEVPISGNPAQQTYFQ
                     TLPVFEKMHQIEVKIKDLQSKKIDLLLVYCNSLSASIDFDFLAKKYSMKIITPFQVYA
                     DLAQKHERIGVLTANGQGAAGIESVLVQHHSTIRVYNVTNLDWVHAVEAKIHPSIIVE
                     QFGLTDSMSFFMKNQVEAILIGCTHFPYFLTEYQSRTRIPCINPDDALIEKVKSIL"
BASE COUNT         3276 a         1744 c         1435 g         3524 t
ORIGIN      
        1 aatacctacc ttcatgtgta ggttttaact tctcaaatga ttgggtaggg agatagggat
       61 cttcctttaa agtctgaata accttctcaa attgaggttt caaattagac tgtttgattt
      121 tctttaaatc agctttagcc gagtttttaa tcatcacaga atagttattc attataagtt
      181 atccggttct gttgcgaagt ttgaaaatca aacgcgccct ctctgaactc caaatatctt
      241 aggctgttat tcccattaat accttgattt cagtagacac cgaaaagtcg aagagcgttc
      301 catttcttcg gttcttttta tatattcctc gaatggtctc catgccctta atcgtggaag
      361 aggctgtacg gagactttga taaaatttat ttcgtcgttt aataggtcga tggtcttgtt
      421 ctattaaatt gttaagatac ttcacagttc ggtgctctgt cttagtatat aaacccacac
      481 tctgtaactt tctaaaggcg gagccaagag aaggtgcttt atcggtcaca attgctttcg
      541 gctcaccaaa ctgtttatgg agtcgtttta agaaagcata ggctgcttgc gtatcccgtt
      601 tctttcgtaa ccagatatct aaggttaagc cgtccgcatc aattgcacga taaagataat
      661 gccaacgtcc cttaattttg atataggttt cgtccatttt ccatgaatag aaggattgtc
      721 tatttttctt cttccaaaga taatagagga ctttgctgta ctcttatacc caacgataaa
      781 tcgtagtatg acaaacattt attccacgat catataacaa ttcctgaact tcacgatagc
      841 ttagattgta acgcaggtag taaccaacag caacaataat gacgtctttt ttgaattgtt
      901 tgcctttaaa atgattcatt actctgtcct ctctgtcttt tttctcaatt ttacactaaa
      961 atagattttt tggaaaactt tgcaacagaa cccttttatt gaaagtaaga agaacaggct
     1021 tttttattag aggcgttcaa aattgtttga gcggttagct tttatttttt agaaataatt
     1081 tcaaaagtat ttttgttgtg taaagaaagt tgtgccaacc actttatttt taatgtttca
     1141 ggatatttat ggaaatttag cttatttgca ccaatcacaa tggctccgaa tgaaaccagt
     1201 attatctcat agccaagatt ttgaatatcg gttaaaacat catccatttt atcgaaagat
     1261 tagatattat ttttaccaat tgtatttgtt aaagttgtat ttccaacttt aattacaatc
     1321 ctgtctctat gaatgttcat caggatattt ttacaatttc catttgatgt tttgtatagg
     1381 gtaataactt tgctattatt tcatttgttg aaatcttcaa atatccagta gtagtcccat
     1441 cactacaacc ataccacctg tcatccgtta catctttatc aaatatgatt ttaattttat
     1501 tctttgtatc aattagtgtg ctaaaaactg tagctgctcc aattttggtt tgtagcatac
     1561 ttgtcatatc ttctttggaa gcaaaagaca ctcttgagat tcctaaagct ttactaaaat
     1621 ctttagattt aaatggtttg tcgccactcg taataaataa atagtaattt gttttttgtc
     1681 tattacacaa aaagagtgtt tttaccatat gcatatctaa tttcaaattc acctgttcgc
     1741 aatcttccat agttatcact tcgtctgttt ctacacgttc atacgatatg tctaaacttt
     1801 ctaaagcagc gtaaactttt tcttgtaaaa gtgtctcata tttttgaggt ttattggttt
     1861 ttatttcact aacattaatc aatctgaatc cttctttcac ttaatattta ctacaaagaa
     1921 actttatcat gtataattac atcacaaaag agaatatatc agatctttag tatgataaaa
     1981 ccagatatta atgaggtgta agcatggata tgaatattca aaagtatatt gcttttctaa
     2041 aaacagttga attgaaaagt tttactaaag cagctgaagc tttaaattat tctcaatcag
     2101 gaataagtag gatgataaat gatttggagg atgaatgggg tgtgacatta cttgaacgga
     2161 accgaacagg tctacgtcta acttctgacg gaattaacat ttatccatat gtaaaaaatt
     2221 tatttgacga atatgaatct atgcagagaa agctagaaga actgaacggc attaaatttg
     2281 gcgtaattag aattgggacc ttttcaagtg tggctacgca ttggctacct caacttatct
     2341 taaatttcca aaataaatat ccaaatataa aatttgaatt attacttgga gattatgatg
     2401 aaatcgaaca ttggattctt actggtaggg tagattttgg atttctaaga ttgcccacac
     2461 tgccaaaatt aagtacgcaa tttatacatc aagatgattt caaagttata gtaccgaaga
     2521 accacttttt agaaaagaaa gattctgttt cactcaatga tctctctggg ttacctttta
     2581 ttctcttaga taaaaatgga aactccaata tcactacatt atttgaaacg atggactttt
     2641 cacctgatat taatttcact acttgggatg attatgcaat tatggccatg gttgaaaaag
     2701 gattaggtgt tagtatactt ccggaattaa ttttacaacg tgtacaatat gatctaacaa
     2761 ttttagattt agatgttcca ttatatagaa aaatagggat tgcatacaaa gaagaaaaaa
     2821 atttatcttt agctagtaaa aaatttctag atcttttatt ttttgaataa ttcatcaaaa
     2881 gtcttagctc aaaatatatt tacaatcata aaatcaaatc gttttcagac tattttgata
     2941 aaatacgaga tagagcctaa tatcattacc tgaccactac cgatcataat aattacttat
     3001 aacaagattg agtttatccg aaactattcc aaaagaaatt tatatactaa tcacacttca
     3061 ctaaaattat ttcaccaata caaatatttt cctataagca agtctaaaaa aactgctata
     3121 agacaaaaaa atagcgattt acgatttttt taccacaatc agtatcgagt ttaatataat
     3181 agtattatca aaaagggagt gtgatcaaca tgcaaaatat aaaaaaaata ctattagtag
     3241 aaatgaaaca agttgaaaga aacgaagctg ttaactgtgt tgcaggagct atcagcgaag
     3301 gactagctag tattgatttt gaagctgcta gggtctaatg atatgtctac taaaaacaaa
     3361 tccaaaggac ttatagtatc aactattatt ttttcatgct ttattctatt aggtatccat
     3421 aatttcagca caaacctttc aatcccaaaa ttattattaa acagtttagg gatcttcttg
     3481 tttatccttt tactctttct ttcgataaga gaattattca aacggtaaaa actatactgt
     3541 ttgttgcaac aattttattt atctaataaa aaaagatagt cttcaaagaa aaagctattc
     3601 ttaacatatg gcattttcat atttttaatt acctatgacg ttctatggga gatttattct
     3661 cattatgata caaaataaag ttatataatt tgaagaacac ttaaatagcc ttagtattgc
     3721 acacaaaagc cgactacttc aatttatgat gtagtcggtc ttttttaaat attatttgta
     3781 ctagatactc actatcagta gcttcttgta aaacagattt taggattaca atttaaaatt
     3841 catttacttc ctttttaggc atttttacta agaagaacag tgggcttttg cctactgctc
     3901 ttcctcttta tccaatcccg caatctttcg ctgtttccga ttagattctt cagctaccca
     3961 ctgtaaaaca gtcataaact cttttcgagt agccttttct tcaaattctc catagctgcg
     4021 attatatttt ttcattattt cctcagcttc ttctctttca atcaagtaac ttcacatcca
     4081 attcgtttta agtataagtg tctcgcaata taaggtcgat atctgtcaca gcaagaacta
     4141 ttattgtaat ctggttgata tagaatttta ttactataga aaaaattaaa tactataatt
     4201 taatctaaaa aagctattgc atactacaac atatttgttg aaatgataga ataaaagaat
     4261 aattttaaca aattagagaa gcttgtttcc atttttcata gtgtaaaaag tttttcaatt
     4321 tattctgtgg ctgataaacg tggaagctga cttagtatct ccgacatggt agttagttct
     4381 gttaaatggt aatgcccatt tggtaatgtc tctattaaaa ttttgttcgc aacaaatacc
     4441 gcgatttctg cagtcatttt tgcttcttct tctccaaaga gaacaatttt ttcaggatct
     4501 ttaacttgtc catttgatac ttttacaaca aaagaacgat ctccgactaa tcctcgtttc
     4561 atgaaccata cggggctctt tattactctc ggatttttaa aaagtcttgc tagtccaact
     4621 ttttttattg ttcttaacaa tgcagtcaac caattttgat ccaaaccgag aaatgttaca
     4681 taattgcgtg aaggatctgc agaaaaaagt aaatgctgat cggcaaagcc aaagttagca
     4741 ctgcgacgct tcccaaatgg tggtccaaaa tccgtttttc gactatagac aaaattttca
     4801 attggttgtg cccatttagg atgtatatac gtacgattta attgctcaaa ggtccatctt
     4861 actgctgctt caccgtgacg atcacctatt cctaaaagga catcaattac tatcatttga
     4921 gcagatggct ctttcttagt gtacgcggcc gcggctaagt tggtaacacc aggtgctaac
     4981 cctaaaccaa tgactctctt gcttttttca ggaatgggta gcaaattaac ttttttcaaa
     5041 aaatcactat tggcactgat atccaaataa tcaattccta atttttgaca ttgttcgaca
     5101 aactgggtat ccgtttggtc caaacaaaca attaccatat caagatcagc aagtttttca
     5161 ggaataacaa atttattcaa gtctacttca ctctgttcta gaatgggtaa aaattcttgg
     5221 gcaaatactt ccattttttt taaatttctt cccattaaaa caacgaaata cccatgattt
     5281 gttaactctc taacattacc ttttccaacc tgtccatgac cgccaactac tgctatcgtc
     5341 ttcatctttc ttctccttta tctaccaagt taattcattt ttaccgatag gctaaatcgt
     5401 ataaaaacta accactttta atcattattt tattcttagt tttatgtgag ataaattaac
     5461 cattttaagt ttacaaatgt aatcaatttc tattacttta taagtatcgt ttacattcgt
     5521 caacgataga gcttgaaata tttttatttg aaaattttgt atttagatga ttatgaacta
     5581 atacagtgtc ggaggatacc tatgtattta aactaaacta ttttagaaaa accagtaatc
     5641 ctttgaattt tctattggaa aatgaagtaa aatattataa gaataacctt taaagttggg
     5701 caactattat tcaaacatct aaacatactc ctaagagaaa tagatatata atagactaag
     5761 gaagcgtttt aaatattgta gaaaatagag aaaatttatc agatcttcgg tttaattttt
     5821 acagcgttaa tcgctcttga tatgaatgag ctctgataaa gccgaagtaa attggcgatt
     5881 tgaaaagttc aattatttga gaatagaata ttttttatat agaattgagt tatagttaag
     5941 ggggaatagg tgtgttaaat aaaatgtcaa tttcaactgt ggaagtcgat ggtggtcgtg
     6001 aaaatgagcg ataatttatt tttagaagta caaccagaag tatttaagca agtacctcgt
     6061 ttaaaggatg gattcttgtt aactttaaat gatggctcta atcaattttc ttcagcagag
     6121 ggatgctgta tgattggtga tcatttttta ttagtttcca cacaacatta tattccagca
     6181 tatgatttaa caataccttg cgaagaagca acagttaaaa catctgaata cgatactacc
     6241 tttattaatg ggcatttagt attagaggta actccatcaa atcaaacaat tcgtttaaag
     6301 aatgaaagtg gtattttaga tagcaatgtc caagtctatt ttgactcatc agacgcttcg
     6361 taaatctgtt ctaaatatag ttttaaaacc tcgaatattt cttatatcga ggttttttga
     6421 tgaattacct agtgtttacg tgttaaggaa caatcgatcg agcgtcacta aaacaccgtc
     6481 ttcgtcattt gaaggacatt gatgcttagc aacttgctta acagcttccg ttgcattatc
     6541 cattgcataa ctcagaccac aatactccaa catctcaatg tcattgcccc catctccaaa
     6601 agcaacgcac tggtcaggat taatccccaa gcgctcaact aacattttca agccgaaagt
     6661 tttatggcac tcagaaagaa tcaagtctac agaaccatga ccactactgg taggtactaa
     6721 tagcccggct aatttttcac ggaacaattc aacgtaaaat tcggtttttt caacaggcat
     6781 agtcggagca aattttaaaa ttttatcttg aacttctgca aaatcagcta cccattctaa
     6841 tctatggtaa tagatattgg ttagatcaaa aaatgcttga ctcacctttc ctctttcaca
     6901 gtaggcacta ttcagaccac acatcacatt ttttacttca ggatagtctt tacataaatc
     6961 aaggaccacc tcgatcgctt ttctaggcat atcggctgaa aatagtacct ctgttttatc
     7021 tttaacaaaa gcaccatttt cagcaacaaa acttaattca tcataataat ttggaaataa
     7081 cgatctcaat tggtagtatt gatttccact tgcaacaaca aattgacaat caacagcttt
     7141 cattcacgat aaaacagcct tgaaccttgg gatatcgtat gtgtaatcag tacgagcaaa
     7201 tgttccatcc atatctactg ctaccaactt tagattatga ggattcttat tcataaaaac
     7261 actcctgtcg cctttgatat cttcattata ttgaaaaagt attcagacct aaagcagcct
     7321 atttttctta ctccgttaaa aataagaaca tttctaattt cagttaacta tctagataca
     7381 tacaagccat tttttgtatt aaaagctacc gacctgtctt tctcctaacc cacaggtcag
     7441 cctaaatcag tgttgacata agatttttat catttttact ttaactgatt ttctataact
     7501 ggtacatcct tggtcagcta gtatcataag ttgatcctaa gataagcacc ttattactga
     7561 atgtaataca caaatttgat ctggataatt cataacctag aaataggcca tcaaaataaa
     7621 aatattactt ttattcatgt tcaatcaggt aataaaccat tcatgaatta tttttttact
     7681 aaatgcgtta tagggtatgg tttaccaaaa ccatctaagt cttcttcaga ttcaatttca
     7741 aatccatggc ttaaataaaa atgttttgca tatttatttt gactattaac atcaattctt
     7801 tttatatctt cagtttcaat cagctcaatt aatatttgac taccatatcg cttaccaata
     7861 aaatctggat caagaaatag cataactaat tcttctgcac ttacaccact aaaaccgaca
     7921 atttctccgt tatcttccca taaataaagt ttaacctcat ctaagttctc aggaatgatt
     7981 gctttataaa aattaaaatc tgcttcagct agaaaatcat gggtttgctt tactgacttc
     8041 tcccaaattt ctaaaactaa tttataatca tttttatttg ctattctttt gtttaacatg
     8101 ctcttattcc ccttttctta taattttaac tttatttgca agcatagtta gtcaaacata
     8161 aagctaccgc tatattttat ttaaaaagga gaattcgtaa tgactaaaat ctattttatg
     8221 cttacttcta atagtggcgt tggttctacc acaatatttg cgacccgcgc tagacgtaac
     8281 attgatgatc ttttagcttt tttctctcag tattatgacg tatcagcaaa ttataccaaa
     8341 gataaggata tgattgatct attggtgcta ccagagccct ttggaccgtt taataatgag
     8401 cgtaatttgc cggtcattca gattccgact attttatttt tggagagaga ctttgaaaaa
     8461 ataaaaacac acattgatgc ttatttggct aaaaaataaa acaaaaaaat cctaagttat
     8521 tgtctgtgtc aaaccaatgc ttattaaagt attgatttga ctttttctat taatgcatca
     8581 tctggattga tacaaggaat tctcgttctg gactgatatt ctgtaagaaa ataaggaaaa
     8641 tgagtacaac cgatcaaaat cgcttcgact tgatttttca taaaaaaact cattgaatcc
     8701 gtcagtccaa attgttctac aataatacta gggtggatct ttgcttccac cgcatgtacc
     8761 caatccagat tcgtgacatt ataaactcgt attgttgaat ggtgctgcac taaaacactt
     8821 tctattccag ccgctccttg accattagcg gtcaacacac caatacgctc gtgtttttga
     8881 gccaaatccg catatacttg aaatggtgtg ataatcttca ttgaatattt cttagccaga
     8941 aaatcaaaat caatcgatgc actcagtgaa ttacaataaa ccaacagtaa atcaattttt
     9001 ttactctgta gatcttttat tttcacttca atttggtgca tcttttcgaa gactggaagt
     9061 gtttggaaat aggtctgttg agctggattt cctgaaatag gtacttctaa caattcacag
     9121 gaaatttcgt tcaatagatc agctcccatc ctagtatcaa taggtgttcc cgccattaca
     9181 gccactttag gcatactgat cattccttca aaatgaattt ttgcctatta tactctaaaa
     9241 atcaaatttg aagaatagcg atccggaagt taaactgaaa attctaaagt aattctttga
     9301 tttatttaga ttcaacttaa catctttagt gtactaataa aaaattcgct caatatttct
     9361 agctatactt tatatcccaa tatacataag cttccctatt tttacaggtt acaagtgaaa
     9421 attaaactgc ttatattgat tgcatttaac tactaaggat gatgcttgtt gttagtatgt
     9481 cagaaaaatc caacagtata gttttgtcag cacattcatc attctttcct gtaaaacaaa
     9541 aagagagtag acctattctt ccacttcatc atcgttatct aaaacagcaa ttttctgctg
     9601 taaaatagtc ataaattttt tctagtagcc ttctctttaa actcatcata atctcaatta
     9661 tcttttttta agatttgctc aactttttcc ctcttgatta ttgcaattca caccttataa
     9721 agttttaaac taatgggaga cgtcatctag cttaacttat agtccctgct ccgtttaatc
     9781 tacacttgtt atcttgtatt attgataaaa gatagactag ttacggattg aaattaggtt
     9841 gcaattctaa aaatccagta gtctaaaatc atgcttatta atttacaata tcttaaaaaa
     9901 gggttttaac ttaataacag aaaaaattat tcatgggcga aaaaatgatt aggatggcgt
     9961 aaaagttaaa gatcttagt
//
LOCUS       DACDLI010000081         9960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDLI010000081 DACDLI010000000
VERSION     DACDLI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.081"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            91..840
                     /locus_tag="IV353_002274"
     CDS             91..840
                     /locus_tag="IV353_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9010475.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            831..1616
                     /locus_tag="IV353_002275"
     CDS             831..1616
                     /locus_tag="IV353_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9010476.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            1585..2301
                     /locus_tag="IV353_002276"
     CDS             1585..2301
                     /locus_tag="IV353_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9010477.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2403..3422)
                     /locus_tag="IV353_002277"
     CDS             complement(2403..3422)
                     /locus_tag="IV353_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9010478.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(3441..4499)
                     /locus_tag="IV353_002278"
     CDS             complement(3441..4499)
                     /locus_tag="IV353_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9010479.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(4720..5868)
                     /gene="nagA"
                     /locus_tag="IV353_002279"
     CDS             complement(4720..5868)
                     /gene="nagA"
                     /locus_tag="IV353_002279"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9010480.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(5944..6780)
                     /locus_tag="IV353_002280"
     CDS             complement(5944..6780)
                     /locus_tag="IV353_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9010481.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(6964..8181)
                     /locus_tag="IV353_002281"
     CDS             complement(6964..8181)
                     /locus_tag="IV353_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9010482.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            8564..8647
                     /locus_tag="IV353_002282"
     tRNA            8564..8647
                     /locus_tag="IV353_002282"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8598..8600,aa:Leu,seq:gag)
     gene            complement(8887..9885)
                     /locus_tag="IV353_002283"
     CDS             complement(8887..9885)
                     /locus_tag="IV353_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010483.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         3188 a         1989 c         1665 g         3118 t
ORIGIN      
        1 ctccagaaca cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct
       61 gccatattga tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc
      121 tctagcattg ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt
      181 gacccgacac atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat
      241 tttatcgttg tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc
      301 ggtcttcctt tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc
      361 tctgaaaaag aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat
      421 caagagatta ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc
      481 aaagatggac aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata
      541 ggcgccgttt ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa
      601 atagcagaac agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta
      661 gccatctctt ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac
      721 caaacagagc agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg
      781 ttttgtttaa agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa
      841 atgggataga aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag
      901 cagtcgcaac tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga
      961 cgaaacaata tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg
     1021 tcatttctgg tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc
     1081 taaatcaaaa ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa
     1141 ttgccggttt tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt
     1201 tagaaagcga ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc
     1261 aagtgttagg gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa
     1321 acggacaggt ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg
     1381 tctgcgatga cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt
     1441 tttcgacgaa gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac
     1501 gccacggtgg atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag
     1561 aattaccaaa aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca
     1621 atggttgcag aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa
     1681 agtttcagaa atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg
     1741 ctgcttgatg tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag
     1801 caaggaaatg atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag
     1861 aatgtgatcc gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt
     1921 cagctggcga tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc
     1981 gatcagaaat tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca
     2041 ttcatcgatt tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt
     2101 ctaaaagaag atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt
     2161 atatccgtca agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc
     2221 aaatatttgg aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa
     2281 aagctcacag attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca
     2341 gaagtaacaa ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt
     2401 tcttatctta attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct
     2461 attatttcac gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta
     2521 tttgaaccta catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata
     2581 gcagggtctg gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt
     2641 gcaaaaacat cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct
     2701 tcattatctt gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga
     2761 gctaaaaatc cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata
     2821 ctgacaatat cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta
     2881 gcagcttctg tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa
     2941 acagcttccg tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt
     3001 tcacgactaa acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta
     3061 tacgcagtat ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt
     3121 ggcgaatacg atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg
     3181 atatgattga ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt
     3241 gcaagattgg tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt
     3301 gctgacacga tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa
     3361 atattcaatt tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc
     3421 atactttctt cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca
     3481 acgatccata caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag
     3541 gctaaaagtg cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt
     3601 gtagccatca tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt
     3661 gccgtcaaaa ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga
     3721 ctgtaatacc atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt
     3781 gcgccgattg ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga
     3841 aatagccacg gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct
     3901 gcacctgtaa ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca
     3961 aaagtagcga taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc
     4021 aagccactca tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa
     4081 attgaggaat acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa
     4141 aaggtcgtag aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag
     4201 cttaagaaaa aaggttttga aactagaata agaataatat aaataactaa tccccaaatc
     4261 aagacattga ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat
     4321 gccagtgctg gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca
     4381 gaaaaaatcc ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg
     4441 attgcactga caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc
     4501 tttctcctcc taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac
     4561 gtattaacat ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag
     4621 aaacttttga aacttatgta aagtgatttt tactagaaca caaaaaaaac cttaaaacca
     4681 aacttaagaa gtttagtttt aaggttaact taaattatat tatgcgtgga aacgttccac
     4741 gccatctaga taagttgctt ccaatgtcat atcagggttc aatacgataa agtctgcatc
     4801 gcgtccttga gcgatttttc cacatgtatc atcgatttta cagctgattg ctggaatcag
     4861 actagccatc atgactgctt gttctggtgt tgctaatccc cagtctacaa catttttaat
     4921 tgcttctttc aatttcaata tacttccagc taagttgcca gattctagac gtgctgttcc
     4981 ttcttttact actactggaa attcgcctaa attgtaattt ccatcaggca tcccaccggc
     5041 catcatacag tctgtaatta atgcgacatg atcatgtcct gctttttcca ttaggattcc
     5101 cgcagcattt ggatgtacat gatggccatc acaaatcaat tcagaaaaga catgttctaa
     5161 agataataac gcacccacca tacctggttc gcgatgattc aatccacgca taccattgaa
     5221 agcatggaca aatacacttg cgcctgcttc aacagctttt tgcgcttctt ctagtgtagc
     5281 attactgtgt cctagagaaa caactactcc ttcttcagtc acttgtttga caaactcttc
     5341 tacaccttga cgttccggtg ctaaagcaat ttttttgatt aaaccacctg aagcttcttg
     5401 ccattcatgg aaagtatcca aatcaggatc cccgaaataa cttgggtttt gtgccccttt
     5461 gtgttcttct gtgaagaaag gaccttcaaa atagattcct ttgattttag ctcctggtgc
     5521 ttcctgatac attttgccga ttgtctcagc tacatctttt aaacgttctt tgcttgacgt
     5581 caatgttgtt ggtaaaaatg aagtcactcc acaagataac agaccttcag acatcacttt
     5641 gatgccttct gcatcattat ccattacatc atgattcata tagccgtgaa tatgtgtatc
     5701 taccaaacca ggagcaatcc atttgccgct ttggtcaatg atgttgactt ctccctcagg
     5761 tagttctttg gtgtattcac caaaaagtcc atcggtaatt tcaagatagc ctgcatgttt
     5821 gacacctgac gctaaaaaga atttgtcagc atagataaaa gttctcattc tattctctcc
     5881 ctttgttttt ctacctctaa agtactctta tttagagaag aagtcaagaa tggtctacac
     5941 catttacaaa ttttttgaaa tttgtttcac agcttgtgct aagccttcat agaatgctgc
     6001 tctttctggt tcttctttac gcttgtatat taagctttta agcacaaata aatcattgag
     6061 gtaatagtag ctattcttct cttgagccca atagattcct tgattcacct gttctaacgc
     6121 ttcgtcgatt tcattttgat ggaataagaa atttccataa ttataaaaaa tttgcgaaaa
     6181 ttcacttttt gatcgttcat cagctgattg atgcagcagt tggatgcttc gactcaaata
     6241 gtagcgtgct tcagccgttt ttcctgccac accatatatt tttccgatac aactaatcag
     6301 ttgtatttct gtgtcagaaa catggattct ttctttttgg ctagagtagc ttaatgcttc
     6361 tttcaaataa taaagggact gagagatatt ttgacgcaac gtgaattcgc aaacaccgag
     6421 ataataataa tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa tatcagaaga
     6481 ttttagtact tgttctaact tccgataatc acgtttttgg aaacaactat ccaacagttc
     6541 aaataattta tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat gatcaatcgt
     6601 tacccctagc cgttgacaaa tctgcatcat caccaataca ttcggcagtt cctcattatt
     6661 ttcgatccga cttaatacac tttgtgaaca aatatcatgc gaaagcattt tttgcgtcat
     6721 tttttgttct tttcgaatct ctttaatgat cgttccaaag gactcgatta acgtttccat
     6781 aagtatcctc cccaaaatat gcgtattcgc attctattcg tattttactt taggaagtcg
     6841 caaacttcaa gaaaagactt gcaactaaat aatatacttt agttatttaa gaaaactcca
     6901 ctagaaagag agcctcagtg tgtttgtctg tggccctcct actgaaaaat ttggttttta
     6961 aaatcatttt tggcgatagg ttgctaggaa ttctctcatt ttcttagctg tttgtttcaa
     7021 atcatccatt ttaggaagat aaacgattcg aaaatgatct ggctggttcc aattaaatcc
     7081 accaccatgt acaagtagaa tatgatgttc atgtaaaaag tccaaaacga atttttcatc
     7141 atccaaaata ttgaatcgct ttgtatcaat ttttgggaat atataaaaag cagcttttgg
     7201 ttttacggct gaaagaccag gaatatcatt gattgcatta tagatatatt ctctttgttc
     7261 atagatcctt ccgcctggca ggagcagctc atcgacactt tgataaccgc ctagcgcggt
     7321 ttggataatt tgctgagaca atacattgga acacaaacgc atggaggaca acatattcag
     7381 cccttctata tagcctttga cgtgtgactt atctccactt aggaccatcc agcctactcg
     7441 aaatcctgct acacgatgag atttggatag accgttcagc gtaacaacga acaaatcagg
     7501 cgctaatgta gcaatcggaa tatgttctag accatccatc actaagcgat catagatttc
     7561 gtcggaataa ataatcaggt tattttgtcg agctacttca acgatttctt ctaataattc
     7621 ttttggataa agtgccccag taggattatt aggattaatg ataacaatag cttttgtccg
     7681 actcgttatt tttgatttga tatcatcaat atcaggatac cattctgcct cttcatcgca
     7741 aatataatga acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca acggatagtc
     7801 cggcattgga accagtactt catctccatt gtccagcaac ccctgcatac acatcgtgat
     7861 caattcgctc actccgttgc cggtgtagat atcattgatc gtcacattcg gaaaatgttt
     7921 cagttggcaa tattgctcaa ttgcttttct agcagaaaag atccccttgg aatctgagta
     7981 gccttctgag tcacgggcat tcatgatcaa atcacgaatg acttcgtttg gtgcttcaaa
     8041 tccaaaaggt gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct cttcttgcat
     8101 ccgctccgct tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt ctaatttgtt
     8161 ggatttttca aattctctca tcacttagcc aatccctcat ttcaaaaagt tagaactttc
     8221 caaatttttt gttatttact atttcaatat catactccat cctagaaaat tttgccactt
     8281 atttaggaaa aacgtaagta ttaaaaaaac acaaacaagc gttgagaact taattgtcat
     8341 gatagtcatt ccatagattc gctgcccaat ccattgcaat tccacaaatg attcctagca
     8401 taaaaccaag caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag atcattccca
     8461 gtcctgaaaa ttctccgtca tatttctcca tcttaataag tcctcccatt tttgagcttg
     8521 acaactatgt aaaacctcgc tatcattagt gtgttattat tacgcagatg tgttggaatt
     8581 ggcagacaag catgattgag ggtcatgtga gcgttagctc gtgtgggttc gagtcccatc
     8641 atctgcacta caactcgtga agttaatttt gcttcacgag tttttttgtt tgaacgtttc
     8701 tttatgccat tatatacata tcactgtatc ctttacaaaa agaacgtaca atcatgtaaa
     8761 cagcctgagg tgagtatatg aaggtcggaa acaagctact cgtagaaact tgcaagtgtt
     8821 aaaacaatta atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg agggattttt
     8881 tgtatattat ctgtttatca ccgttattat aaaggtacaa ataccaagca cggttaatcc
     8941 aaattgcata aattcatttt tttgtacact catgcttttc attagctctt taactgccat
     9001 tagtactaag atggcgataa caacataaag tatatagtac gtccgcattc cggaagccca
     9061 tactgtggga gaaaatccca taattaacct tgaacaaaat cccattatca tcaaaataac
     9121 taaaaaaata gaagttttct tattgtcaaa acttaaataa attccaacaa ttaaacatag
     9181 taataatgcc aagactacta aagtagctat ccaagtacct ggataaaaaa gtgacagttt
     9241 cgttcctaac tctgtaaaga gattattcag gttactacta ctccaaatca ttgccctttt
     9301 atttccattg acgtaagtaa agctttgacc cattgtatta ccaaaataaa taatcaggtt
     9361 taagaaaaac ggtaatgcag tcaatatacg cacataatag ttttgacatt tcctatatgt
     9421 tagaaggaaa attaagaaaa atagcaataa aaataagata ttcatgtcta aaaaaaacgg
     9481 ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat ttttatattc
     9541 aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag ttgtcaaaga
     9601 gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat tatatcgtcc
     9661 attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg cgttgacttg
     9721 ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt ttctatttat
     9781 ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa atgctactgg
     9841 ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg tcaatggtat
     9901 taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct caaaaacata
//
LOCUS       DACDLI010000082         9819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDLI010000082 DACDLI010000000
VERSION     DACDLI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.082"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..495)
                     /gene="rlmH"
                     /locus_tag="IV353_002284"
     CDS             complement(16..495)
                     /gene="rlmH"
                     /locus_tag="IV353_002284"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9010484.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            923..2212
                     /locus_tag="IV353_002285"
     CDS             923..2212
                     /locus_tag="IV353_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9010485.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2251..2856)
                     /locus_tag="IV353_002286"
     CDS             complement(2251..2856)
                     /locus_tag="IV353_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9010486.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(3052..3516)
                     /locus_tag="IV353_002287"
     CDS             complement(3052..3516)
                     /locus_tag="IV353_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9010487.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3538..3858)
                     /locus_tag="IV353_002288"
     CDS             complement(3538..3858)
                     /locus_tag="IV353_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9010488.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(4001..5080)
                     /gene="pepA"
                     /locus_tag="IV353_002289"
     CDS             complement(4001..5080)
                     /gene="pepA"
                     /locus_tag="IV353_002289"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9010489.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5280..5630
                     /locus_tag="IV353_002290"
     CDS             5280..5630
                     /locus_tag="IV353_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9010490.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            5815..6768
                     /locus_tag="IV353_002291"
     CDS             5815..6768
                     /locus_tag="IV353_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9010491.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            6781..8568
                     /locus_tag="IV353_002292"
     CDS             6781..8568
                     /locus_tag="IV353_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010492.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            8555..9757
                     /locus_tag="IV353_002293"
     CDS             8555..9757
                     /locus_tag="IV353_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010493.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         2872 a         1853 c         1808 g         3286 t
ORIGIN      
        1 tcttaaaaaa tatcattatt tgtggtatgg agcccccgcg ttgatccgga aggcacggta
       61 gatttgctcg acgaggatca ggcgcatcaa ttgatgtgga tacgtcattt tgccaaaaga
      121 gatctgagca ttgcttcttt tcatgacggc ttcgctgagt cctaaagaac cgccgataac
      181 aaaaacaagg tggctttttc cttggatacc taattggtcg atttgtttag caaaagattc
      241 gcttgtggga tttttccctt caattgctaa gccatagacg tattcttgtt ctttgatttt
      301 cgctaagatc cgttctcctt ctttttcttt tacttgtctc atttgcgctt cgcttaaatt
      361 ctccggggct ttttcgtcag gaacttcgat caattcgatt ttcgcatagg cattcagccg
      421 tttgacatac tcattgatac cttggatcaa gtatttttct tttaattttc ctactgaaat
      481 aattttgata ttcatgtttt ctctcctttg atcttcacgc tcactttgtt tattataatc
      541 aattttttcg tctatatgtt tattttgact atttttgtta aaaagatttc cccaacagtt
      601 atgcacaaca tccacaggtt tatccacaga aaacagcgtt gttttacctt atttcatcac
      661 attcattagg caaacatacg ttttataaga atttatccac tttatacaca ggctgtggat
      721 atgttttatc ccttattttt ccaatcctat ccgcgtagtt ttacacaata tctgtggaaa
      781 aagaaaatca aatattttca tctgaaaaca atgatttttc ctaaagtttt tcataaaaag
      841 ttcaaagtct caaagtagac taaagtcaac aaataagact atactaacag tagataaatg
      901 aatgtaattg gaggaggaac acatggatag aaaaaatgtg acaccaaaga tgaaaaaaaa
      961 taaaaataat agcctctggc gtaagttggg tctggggctt gttggaggga tcgttggagg
     1021 actactgaca gctggcattt tttatgcagc gatgggcact ggcaacaata gttctactgc
     1081 tacaagcggc aatcaaaatt ctgccggtga aacagttgtg gaaaatgtca aagtcaatgt
     1141 agattctgat atcaccaaag cggtagataa agttcaagac gctgtcgttt ctgttatcaa
     1201 cttacaaagt caaaaccaat caagcggttt tggcggatta ttcgggcagc aagaagaaag
     1261 tagcagctca gatgatagta atttggaagc ttatagtgaa ggaagcggcg taatctacaa
     1321 aaaagacggg aatactgctt atgtagtcac aaacaatcac gtagtagatg gacagcaagg
     1381 tttggaagtc atgatgaaag acggtacgaa ggtaaaagct gaattagttg gaacagatgc
     1441 ctattcagat ttagcggtgt tgaagatcaa ttcagacaaa gtcgaaacgg ttgcctcttt
     1501 tggggactca agtgctttga aggttggtga acctgcgatt gcgattggtt ccccattagg
     1561 atctgaatat gcgaattctg tcacttcagg aatcatttct tccttgaacc gacaagtaac
     1621 aagtacgaat gaatcgaacg aaacagtcaa tatcaatgcg atccaaacag atgcagcgat
     1681 caacccggga aattctggtg gtcctttagt gaatatcgaa ggacaagtta tcggtatcaa
     1741 ctcaagtaag atcgcaagta cttctgaatc gacttctaat gtaagcgtag aaggaatggg
     1801 atttgccatc ccaagtaacg atgtagtgaa tatcatcaac caactggaaa aagacggaaa
     1861 agtgacacgt ccagctctag gtatcacaat ggtcgactta tctgccgttt ctactcagca
     1921 acaagaacaa atcttgaaga ttccttcatc tgtaactaac ggtgtcatcg tgacaaatgt
     1981 ccaaacagct acaccggcag aaaaagctgg gttgaaacaa tacgacgtca ttactaagat
     2041 cgatgacaaa gaagtttctt ctggtgtaga acttcaatct gctttgtata agaaaaaagt
     2101 gggagattct gtcaaaatca cttactatga aggctcgaaa gaaaaaacaa ctactgttga
     2161 attgacgatc gatcaatcgg cattaaaaca aaatcaaaat agtgaaaatt aataaaaaag
     2221 aggttgtgac aaagacttgt cacaacctct ttattttgga aatacttctc cgactacggc
     2281 atcctcaggc aatactaaga ttcctttttt ttctggagca tttggcaaat gcaattcacg
     2341 agcagaacaa atcattccat agctggctac ccctcgcaaa gttcctggcc agatcatcat
     2401 tccatctggc atcatggctc ctggttttgc aacgacaact ttttgtccag cttcgatatt
     2461 tggtgcaccg caaacaattt gcaatactgt cccattgtcc acttctgtct cagtaatcga
     2521 taaatggtca gagtcaggat gcgggacgca tgtttttaca tacccgacaa cgatcttcgg
     2581 ttcatgatca gcttcaagct gttcttcgaa tcctgcttct ttaatcaatc gattcaattt
     2641 cgctaattct tcatctgtta agcttacttg gccgttccct tcaattgtta gatgatcgga
     2701 gatatggaaa aagttccaag caacaacttg accttgttca tctttgatcc gagcaacatt
     2761 ccatttacgt tcgacagtgt tttctgttcc tttatcgtcg gctatgataa ccatcaaagt
     2821 gtctccgacg tgtgctttgt tataagcaaa aatcatgttc tttcctccat tatcctttgc
     2881 tgagatttat cttaacaaaa aattgcattc actgcatgag atttttttca tgctttattt
     2941 ccgaagaagc ttcttctgga acaccattta ttcgtaacaa gggactgtga caagactttt
     3001 gtcacagtcc ctttactgtt tgattaaaaa ggttatacaa gttccacttg cttaattact
     3061 cggctgttct tcttcgtcag aaacgatcaa gacatcacaa ggtgaatgac gtatgacgta
     3121 gctggctaca cttcctgtca taaagcgttc gactgcattt agtccagact gcccaaccat
     3181 gattagatcg attgcatatt tttttggcaa tttgactgcc atggattcac gaacagagcc
     3241 gtatgcaata attccttcta tatttgtgta atcaacagat tttccatatt ctttacattc
     3301 ttcgatcatt tgcttggctg tttcagtttg ctgatcgact agattttgat caataggagc
     3361 atagcccata aaattataaa attgctgatc gattacgttt gctacatata ctttcccatt
     3421 attacggcga gccacttcta acgctttttt gaaagcagca agtgcttgtt cactaccgtc
     3481 aatacctact aaaatgttct tatattcttg attcatcttg acccctccta acagatgtca
     3541 gccttttatt acactttcta ggaacgattc gatttcctgt tttgttttac gatctttatt
     3601 cactagacga cctaattctt ttccgtcttg gatcacgaca aaacttggga taccaaaaat
     3661 gttccattca gcggctacat ctatgtactt gtcacggtct acttcaataa attgccaatc
     3721 agaaaagtca ttttcgattt caggcatttg cggtttgata aaacggcagt ctccgcacca
     3781 gccggcagta aagaaaaata cactttttcc agtttctacg tagcctgcta attcttctaa
     3841 actatttggg ataatcattc tttttcctct actttctgtt tatgtatttt tcaattaaat
     3901 tatacacctt tctttcgaac taacaaacat tgatcctctt tttataaaaa aaatctgaca
     3961 aaaggttgct tttgccagat tttatagttt cactatcgcg ttatttcccg tatacgatcg
     4021 tgttcactgt tgatttatct aaacctttca atgtttgaat caacatttct cgtgcagctt
     4081 caaaatcgcg aatactgaac atcgtttgat gcgtatggat ataacgtccc actacgccaa
     4141 tcactgtact tgggattcct tcattttgtg tatgagcagc acctgcatct gtaccgcctt
     4201 ttgaaacaaa gtattgataa gggatgttgt ttgtttcagc gatatctagc aagtactcac
     4261 gcaaacgagg aagcatgatc aaacctggat cttggatacg catcaatgta ccttctccta
     4321 aatgtccgaa tgtgccattt ttcgtcactg tgtcatctgc tgcagagcag tctactgcaa
     4381 agaacagatc tggcttgaat ttattgacag aagctttaga accacgcaag cctacttctt
     4441 cttggacatt cgcgcctgca atcaatgtat gacctaatgt ttcattttgt aacgcttcta
     4501 aagcttctaa taccattgta cagccatagc ggttgtccca cgatttgctg atgatattct
     4561 tcccgttggc tgttttgatc gtttcagcgt aaggaacgat tgtgtctcct ggaagaacac
     4621 cgtaactcat tgcctcttct cttgattcaa acccagcatc gaacaaaata tctgttactt
     4681 ctactgattt ttgtccgctt gttcctcgta ataaatgtgg gggaacggaa gaagaaatac
     4741 atggataatt cttaccagta gaggttttaa gtgtaaaacg ttgagcagaa acaacgtaag
     4801 ggttccatcc gcctagaggt acgactttaa aaagcccgtt ttcttggatt tgtgtcagca
     4861 tgaatccgac ttcatccata tgtgctgcga ccattacacg aggtgcatcc gcttcttcat
     4921 ggtggcgcag cccaaaaatg ccgcccaaac catctaactg taactcatct accagcggtt
     4981 ccatatgact ttccatatag gcgcgaacgt cttgttcaaa accgcttgtt ccttgtaatt
     5041 ctgttaactc tttgattcgt tgaaatgttt tttcttccat atgctattcg tctaaatttc
     5101 agactaaact cctctcagac ttatattgat ctgcggataa aatatgattt tttctgcaga
     5161 ccgatcgtat ttattatagc ttattttttc tggtagcggt tatttatttc aaagaaaata
     5221 tgagagtgtg gtaaaataaa cacaaagagt agcaagtgag aagaaggagg tatatccaca
     5281 tgtatgaaga aagcgaatta agctatttta aaggcgggtt agccgttggg atcggtctag
     5341 gattattgag tggggtagct tcaacgattt ggtatcatcg caaaaaagtg ttatcagcag
     5401 accaagtgct tgaaaatatc aaaacatctt ttttaaaaga aggcgcaatc gaaggtgctt
     5461 ggatctcttt tgaaaaagaa cctattcgga aattcgccgt tcggtcgagt gcttatcggg
     5521 gtgggctttc ccggatagaa gatggcgaaa ctgtttatta tgaattttta gcagatgctc
     5581 atacaggaac cgtattagac atttcaagaa aaaagggcgc aatagattaa tacgtcaaaa
     5641 aaatgaaata acgaatacgc acccactatc taaaagagag gtatcctatc cttcttttat
     5701 agtgggtgat ttttataaaa atagggctat ttcctcttgg aatagctagt atttgtagtt
     5761 attatacggt attcttgaaa taagattagt tttattatat aaggagagtg acaaatggaa
     5821 tcacgtgaaa aaattgccgt gctcattcct tgttataacg aagaggcgac aatttcaact
     5881 gttattgaag attttaaaag ggaattacca gaagcagata tttatgtcta tgataataat
     5941 tcgaccgatc gaacatacga attggccgtt caaggcggag caatcgtaaa gaaagaacca
     6001 cgtcaaggaa aagggaatgt aatccgtcaa atgttttttg atattgatgc cgattattat
     6061 ttgatggtag atggtgatga tacttatcct gcagaagctg cacatggttt gcttgagaaa
     6121 ttacgttctg gcgaagcaga tatggtcatt ggcgatcgtc tatcaaatgg aacctatttt
     6181 gatgaaaata agcgtccttt ccatgatttt gggaataatc tggttaaaaa tacaattact
     6241 tacttatata aaacaaaaat tcgcgatgtc atgaccggct atcgaggatt caatcgaatt
     6301 tttgtcaaaa gcttccctat tatgagttct ggttttcaaa tcgaaacaga attgacgatt
     6361 catgctttag ataaaaaatt caagttcgtt gagattccaa tcgattatcg cgatcgtcct
     6421 gaaggtagcg aatcaaaatt gaatacattc tctgacggat tcaaagtgat catgatgatc
     6481 atcaaaatgt gcaaagatta taaacctttg cttttctttg ggatctggac catcttcttc
     6541 tttttatttg gattggtcat ggggattcct gtgattcgtg aatatatgct gacaagcttt
     6601 attacacgca tcccttctgc gattttaagt actggattga tgactttagc ccttctttca
     6661 ttagtaacag ggttgatttt ggatacggtc gtcacaaatg ccaagaaaga atatgaattg
     6721 aatttatacc atgtctacca cgaatatcaa aataaagaaa tgaagtagga ggcctttttt
     6781 atgcataaga aaaaggagaa tctatttcct actttttttg caactttatt tgcgtcaggt
     6841 tttgcttttt ttacttctta tggtctagga actttgaatc caacagtaat cattggttta
     6901 gccatggttt tgctacttgc ttttaagcaa tatggaatag agttgttttc aataaaaaac
     6961 aaagttgcag ctattgcccg attgtttttt tctgcagctt ttgctcttac actttctatt
     7021 ggcagacaga tacaatttca gtatgtctca gcaaactata caaagacaac gattgctgat
     7081 ggaagtattt tttactttct tgtttatttc tccatttttg tgtattcttt tttattttta
     7141 caatcgattt atttatcaat gccaaaactg gaaacatttt ttactgctca aaacacacag
     7201 gctttctctt caagaaaata ttttttattc tttactgtct ttttagttct gtgttggctt
     7261 ccttatctgt tggcattgta tcctggagtg gttttgcctg attcgttaag ttcggtcgcc
     7321 caatctatgg gtgatgttcc tataagcaat catcatccag tcttattcac cttactggtc
     7381 aagctatttg ttcatgattt accaactgat acgatcaatc acggtgtatt tttattttcg
     7441 atttttcaaa cattgataac agcttcagcg ataagttatt ctctttgctg gctgttaaaa
     7501 agaaacatcc atttttttcc ggtactcatt agtttatgct attttgcatt tgcaccggtt
     7561 tttccaatct acgccttgaa catgcaaaaa gatactttgt tctctatcgc ttgcttttta
     7621 ttttccttag cggtttttga ctttattttt caacgagaaa agccaacgat tctcaatcag
     7681 atttacgtaa tcatccttgc tttactgacc acctatctaa gaaataatgg gatttatgta
     7741 gtaaccggca caggtttgat ttgcggcttg attattttga aacaaaaaag gtatcaagca
     7801 ttctttcaag tggtgctggg ttatacagtg attgctggac tactcatcca cccgttgatg
     7861 gctcgttatg ctattcctac agaaactgct gaacaattag gtgttccttt gcagcaaatt
     7921 tctagaacta ttgttatgga tggtgacttg tctaagaaag ataaagcatt catgaatcag
     7981 ctactcccga taaaagatta tgaagcgtat tctcctagtc ttgcagactc aatcaagtgg
     8041 cataaaaatt ttaacaatga gtttttgaat acacataaaa aagaatttct atcactatgg
     8101 gcaagaaatt taccaagtaa tataaaaatc tatacagatg cttatatttt agaaacgttc
     8161 gggttctggg tacctggtac aaaaaatagt tacggctttt tagatactcg tgtaaacacg
     8221 aataattatg gtatgaagca agtagatttg attgaacatc ttactggatt gtcaggaatg
     8281 aaaaaaatca tcgatgctcg tgatttcttt ggttccggca cattactgtg gttagctatc
     8341 ggcagtgcac ttttagcaat ctttaagaaa aaatatcggt atttactcct ttttcttccc
     8401 ggtatgcttg tttttgctac tattatgatt gctactcctg tggcttttag tttacggtat
     8461 gtgtttgttt tagcattagg tctacctgtt tatatcctct ctcctttttt actagataaa
     8521 agagaaaaaa tgaaaacaga tataggagat actcatgaaa ctatttaaaa agatattttt
     8581 tgcttttgtt ctcttgatcg ctagctattg cgtactactg acaattactt ttttcattcc
     8641 gcagcactct attgaatcga atgcagaaaa atctctacag atggttgaaa aagaaggaat
     8701 gtatccttct atcaatcaag gaaatatgct aggcactcgg ttagataatt ttactgatca
     8761 tttgatgata cgaaaaacaa aagcagatcc tgaattgaac tccttggaga acgcgatgtc
     8821 tatggcggat tatccccgtt attggcatgg ttatcagctt tttttacgac ctttactgct
     8881 agggatgagt ttggggagta ttcgaatgat ttacgcagcc attctttttc tgctgattgg
     8941 attgacgagc tactttttaa ccaagtgtag cgacatctat ttaacgacag ccttgttgat
     9001 ttcgcttgct ataggaaatg ctgcaacctt tttcttcagt atgcaatttt caaatatttg
     9061 gattttgact ttattgtctg tattactatt tttaacaaaa ccacaactct ccaacaccaa
     9121 ccaacaatta ttcttgtatt tctttgtcat tggttcttta gctaactttt ttgacttgtt
     9181 gacaacaccg gtcatctctt gggggatacc tgtgatcatt atttattatg taacaaataa
     9241 gtatttaatg gataaacgca gttcattgag caaacaagta ggcagttttg tttttaccgg
     9301 tatcttttgg ggattaggct atggtttgac atgggtggct aaatggatcc tatcatcgat
     9361 catattgaac aaaaacattg taaaagatgc aatcaaccaa atcctctttc gaacggaagg
     9421 aaacgacaaa tacccgttgc atcgattaga gatgctgaaa agcaatatta gattgatgta
     9481 tccaaaagta gcaatattat tgctattgat tacttgtctt gtttttctct attttgctta
     9541 tcgtaaacga aatagctggc gtccgttcca acttctagtt ttgtttgttt tagctgctgt
     9601 aacgccgtat atttggtaca atattttagc taatcactca cagattcatt attggtttac
     9661 gtatcgtacg caaatcatca caagttttgc tatacttagt gctttcgcct ttttgatccc
     9721 tcctgagaaa tcaaaagacg aactaaatta cttttaagaa taccgatgta ctaattcatt
     9781 tttaaacata aaaactccag ctgtcagaga tagccggag
//
LOCUS       DACDLI010000083         9774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDLI010000083 DACDLI010000000
VERSION     DACDLI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.083"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(238..462)
                     /locus_tag="IV353_002294"
     CDS             complement(238..462)
                     /locus_tag="IV353_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010494.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            662..3430
                     /locus_tag="IV353_002295"
     CDS             662..3430
                     /locus_tag="IV353_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9010495.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3511..3930
                     /locus_tag="IV353_002296"
     CDS             3511..3930
                     /locus_tag="IV353_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9010496.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3942..4412
                     /locus_tag="IV353_002297"
     CDS             3942..4412
                     /locus_tag="IV353_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9010497.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4429..5208
                     /locus_tag="IV353_002298"
     CDS             4429..5208
                     /locus_tag="IV353_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9010498.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5195..6016
                     /locus_tag="IV353_002299"
     CDS             5195..6016
                     /locus_tag="IV353_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9010499.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6033..7082
                     /locus_tag="IV353_002300"
     CDS             6033..7082
                     /locus_tag="IV353_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9010500.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7095..8099
                     /locus_tag="IV353_002301"
     CDS             7095..8099
                     /locus_tag="IV353_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9010501.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8732..9751
                     /locus_tag="IV353_002302"
     CDS             8732..9751
                     /locus_tag="IV353_002302"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9010502.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3277 a         1623 c         2042 g         2832 t
ORIGIN      
        1 aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc gtaatcaatg
       61 ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac agtacctctt
      121 tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt gttgttatac
      181 cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct gtgaatctta
      241 ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata aaaatcctgt
      301 ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca tagccatata
      361 aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc gaaaagtttt
      421 ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt catagcctcc
      481 ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta cgttttttag
      541 aataaaaatt ataagggata agtaggctgc tgtctataat aagatatatt tttcaattct
      601 ttattaaaga ttttattgac aaaatgaatc cgttttctat gatggaggag gggtgaataa
      661 gttgttgaag aaggaaattt tatgttactt agagaaccag acagcctttt tcgatttaga
      721 acatatgaac gaggtcttta cagcacaaag gttagctgat cattttgggg taaaaagaaa
      781 cacagtcagt cattatttga accagttaaa tgatgagcaa ctactagtca aaatcaattc
      841 tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa caatttttta agttacgcac
      901 gaatttttat ttttctatcg atgatctcaa agaggaacag ccttttttcg cgcaaccaaa
      961 agacttattc tcttttatga tcggatacga cgccagtttg aaagaaagta tcgaacaaat
     1021 caaaacagct ctttactatc cagatggcgg attaccactt ttgattacag gcgaaagtgg
     1081 cacaggtaaa agttatttag ttaatttggt ctatcagtat tgtctgcttc atgatctgct
     1141 agaagattct gcaccattca tcacttttaa ttgtgcacaa tatgcagata atcctgaact
     1201 tttgactagc aatttgtttg gacatgttaa gggagcgtac actggtgcag aagaaaataa
     1261 aaaaggggct tttgaagcag cagatggtgg gatcttgttt ttagacgagg tacatcgctt
     1321 aacaccagaa ggtcaggaaa aactgttcac ctatcttgat caaggtgtga tttatcgtat
     1381 gggagaacct aatttgtcac gccggatcaa aacacgtctc ttttttgcta cgactgaaga
     1441 gattactagt catttcttaa caactttcat tcgaagaata cctatccgaa tcgaactacc
     1501 tgcacttaat cagcgtagtc gtaatgaacg gctagaactg atttactctt tttttctcga
     1561 agaacaaaga aaaatgggga gccctttgtc agtcagtgga ccagctttgt cacttttaac
     1621 aagtcagaaa ttatccggaa acataggaga gctgaaaaat atcgtcaaag tttcagcagc
     1681 aaaagcatat gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc atcatttgca
     1741 aaaagaacta cttgcgcagc ctattactaa aaatacagca cagcaaggaa tctatattac
     1801 gcaagaccag accttagagc aattaatgga gaagcaacag cccgaacagc agcgaatcgt
     1861 taaaagtttt gagcaaatcc taattgattt caagaaaaat aactgtctgc tatattcttg
     1921 cgaaggaaaa ttaaagcaag aagtgattca attatttgat tttttgctgt ttgagaccag
     1981 tagacaagaa aaacacgagc tgctggttta ttggacacaa tctatccgag agacctttcg
     2041 acaaatggaa actgcctatc aaatcaaatt cgatggaaac agtgtctatg ccttgagtta
     2101 ctatctctat cagcgaagca ctatcaaatg gttaccggaa gaacatgata ttatccaact
     2161 aatcaaagaa ttagaacaac aagtttctca gtcttatcca tctagttatc actatgtcca
     2221 acgaattctg gaattaagta agatcaaatt agatattgaa gacacaagta tggatcggat
     2281 tttactgacg atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac tgccaaaagc
     2341 gatgattgct gctcacggtt atgcaactgc tagcagtatg gcaaatgtag taaatcgttt
     2401 attacaagag gatctatttg aatcatttga tatgccacta gatgtcacac cacagcagat
     2461 tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt tcaaatggat tgatcatatt
     2521 agtagacatg ggatctctga aagaaatcca tcagtttttt aaaaaacagt tatccgtacc
     2581 gcttttgatt ttgaataatg tgacgacacc tttagcaatc acagtagggg aatgcctgca
     2641 aaaaaatgta tcattagaag agatagcaga agagactgtt cgacagatcc agccagaatg
     2701 gcgactgctc tatccagagg aaaacaaacc caaagcactc attactactt gcttcactgg
     2761 aatcggtacg gcggtccact taagtgaact gctagaaaaa agcttaccta cggcttgtca
     2821 gttgaaaatc atcccatatg aatatcagca attaaaagat aagaaaaaca gtgacccatt
     2881 attttccata tacgaagtcg taggaatgat tggcaccaca gatcctgata tcaccaatat
     2941 cgactatctt tcattagaag acctcatctc gggggaaaag atgtctgtac tagctgaatg
     3001 gttggacact actatgaatg cttcagagaa ggaaatattc aatcagcgca tcattcgtaa
     3061 tttttcattg gaaaaagtac tggattccgt gactattcta gatacagaga aggtgatggg
     3121 agaaatcgat cattttatga gagaattaga ggtgcagttg aatcgaagca tcagcaatcc
     3181 caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt gaacgattga tccgacaaat
     3241 gccaatcgaa acctatcaag gattagagaa atggaagcag tgtcaaaaag aagggttatc
     3301 tgctattaaa tctgcgttta gtgtcattga agagcattat agtgtcatga tccccaattc
     3361 tgagattgcc tatatctatg atattttgag tgaagataca gagtttctct cgatagaaga
     3421 agaattttga acataagcaa gttgttgcca ctaattttgg catgcaactt gcttttttag
     3481 tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag aaattatctt agcttcccat
     3541 ggaaaatttg cttcagggat attagattca ttagagctga tctatgggaa aaatcattct
     3601 attactgtat tcgattgcta tactgatgag aattttgatt tagcggaaac agtaaggcag
     3661 ctttttatga ggtacaaaga taaagaaatc attgttctga cggatctttt tggcggaagt
     3721 gtcaacaacg aattcttaca atatatcaat cgggatcatg tctatctcgt tgccggattg
     3781 aatttgccac tattgattga gctagtatct cggttggacg ctgaagtaga gacaactgtt
     3841 ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa cgagcggctc
     3901 aatcagaaaa tagaagaaga cgaattttag gaggaagaaa aatgattgta ttgacaagag
     3961 tcgaccatcg gttattacac ggacaagtag ctttttcatg gacacaaaca ataggcgcag
     4021 actgtatttt gattgctaat gatgatgtac ctacgaatga gatacgaaag acaacgatca
     4081 aattagccaa gccacaagga gtcaaactag tgatcaaatc aattgatgac tcgattgccg
     4141 cgttgaaaag cggggtgaca gataaatata agttattcat cgtagtggaa tcgatcgagg
     4201 atgcatataa gctggcagaa gcgtatccag aaatcaaaac catcaactta ggagggatca
     4261 aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa tattttacca gaagaggagg
     4321 aacgcttgaa acgattaatt gacaaacaaa tagaagtaga aatccgtcaa gtaccagcag
     4381 ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat gattgtacaa
     4441 gcagtcttat taggaattgt tgcatttgtc gcacaatcag aatatgcact agggacatcg
     4501 ttgctttctc gtccgattgt gacggggctg tttacaggaa tcgtgttagg tgatgtaaaa
     4561 actgggatca ttatgggcgc aactttagag ttagcattta tcggatcgtt ctcagtaggt
     4621 gcttcgattc cacctgatgt tgtgaccggt gggattttag ggacggcttt tgctattaca
     4681 gctggagccg gtacagaaac cgcgctttta ctaggtttgc cgattgctac gttgacattg
     4741 atattgaaaa atatttactt agggcttttg attccaatta tgaaccataa agcagacaat
     4801 tatgcagaag aaggaaatta taaaggagtc gagcatatgc acttgcttgc cggtatcggt
     4861 ctttccttga tgcttggtgt tgtggtaatg gtttcttata tggtgggaag caatacgatc
     4921 ggaaatctat taaatatgat tcctgacttc gttcaaaagg ggttgtcagt agctacaggg
     4981 ttgattccag ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa acaagttgca
     5041 ccatattttt tcttaggttt tgctatagct tcttacttag agattcctgt gaccggagta
     5101 gctatttttg gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt ccgtcaacta
     5161 ccacttcaaa caactgcagg ggaggtagaa gaagatgaag aattctgaga tcaaaaacag
     5221 caaaataacg aaaaaagagc taaatcacgt attttggcgt tcttttcaaa tggagttttc
     5281 ttggaattat gaacgtcaga tgaatatggc ttatgtttat gcaatgattc cgattttgaa
     5341 aaagttatat cagtcgaaag aagaaatggc agcagctttg aaacggcatc tagagttttt
     5401 taacacgacc ccacatatcg ttacactgat tttaggaatc acggctgcaa tggaggaaga
     5461 aaataaggat gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa catctttgat
     5521 gggacctctt gcagggttag gagattcttt cttttgggga actttgcgat tgattgccac
     5581 aggtgtgggt acctctttag ccatgcaagg aaatatttta ggccctatct tgttcatttt
     5641 gattttcaat attccgcaca ttctgtttcg ctatctagca acaggttggg gatatcgctt
     5701 aggaacgggt tttttgaaaa agatccaggc aaacggtatg atggaaagtt tgacattagg
     5761 cgcgtctatt attggactga tggtcgttgg agcaatgact gccacgatga tcaccatcaa
     5821 tattccttta aaaatcggtt caggagaaaa tgctgtgact gttcaaagta tttttgatga
     5881 tatcgttcca aatatcttat gtcttggagc atttggcata gtgttttatc tattgaagaa
     5941 agaagtgaaa ccattaacga tcttgatcgg actagctttc tttggaattt ttggttcgtg
     6001 gatagggatt ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat tatatctatg
     6061 aagaaaagga agtactaagt acgatcttga aaaagaatga tttttcgaca agagaacata
     6121 tgaaaaaaac agtgaattta ctgatcttag caacgggttc atcttataac gcttgtttag
     6181 cagcgaagcc agcattggaa tcttatggtg atcttactgt cgatattcaa gaaccttttt
     6241 attttttcaa ttatggaaaa ctttctcctt caatcgatac tgtgattgct gtgtcacaaa
     6301 gcgggaaaag tgcttctact gttgaagcag taaaaatgat acaaaaacaa gggttacctg
     6361 ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt agaagctgca caaattattg
     6421 atttaggtgc cggggtagaa tcagtaggct tcgttacaaa aggctattct gcgactgttt
     6481 tacagttact actcttaggg ataggaattg gaataagtaa aaataaaatc agcaaaaaaa
     6541 tagaacaaga ctacatgcaa cagttgcgca aaataatcaa ccatttacca gcgattattc
     6601 aaaaaacgga agaatttttt gatggatatc aaagtttatt tcgtttagca cagcgtttcg
     6661 tcaccattgg atatggtcca aactggggaa cagctaaaga agcagagacg aaattgactg
     6721 aaacaatccg cgttccgtca caaggatttg agttagaagc ttatatgcac ggcccatacc
     6781 tggaggcaga tgcttcgcat ttgctattct ttattgaagg ggaaagtgta aataaggaac
     6841 gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg agagactctc accattacca
     6901 caaaaaaagc aagaaacgag aaaacacttg gattagcaat agaatgtgat gaatatctgt
     6961 ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca gtagctaaag
     7021 gtattgactt gaacaaaaag atttttgaag actttgatac agtcttgaaa agtaaattat
     7081 aagaggtgga aatcatgctg aaattcaatg aagaagaaca aatcaaagca ataaaaggcg
     7141 cactagcttt acgtccgcaa gtagaagaaa tcatcgataa actttatgcg gaaaaatttg
     7201 acgctgtcta ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa gctgtcactt
     7261 atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa tattacacta
     7321 ctggaaacaa aagattaaca aaagattcta tcgttatttt gtcctctgtt accggtacaa
     7381 ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca ctgattggat
     7441 ttatcgataa agcgaacagt aagttgtctc agctatgcga ttttgtggtc acttatccag
     7501 ctcctggaac agaacaaatc aaatttttta tggcagcaga tcgattgatg tatttgaatg
     7561 gagaattcga agcttattca gaatactatg aacagctcga acgttattta ccaacagggt
     7621 tagtggaagc agagaaaaaa gcagatgcat ttggcttatc atttgcagaa aaacatcgac
     7681 gtgatagtat gcattatttt attggagcag ggaatcaatg gggagctgtt tattcatatg
     7741 ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa atctatccat gctgccgagt
     7801 ttcttcacgg gacgttagag atcgtagaag aaacaacccc agtcacatta tttttaggcg
     7861 aagatgaaca gagagtgtta tctgaacgtg tggctaaact tttgccaaaa atttgcagca
     7921 attatacatt gatagacaca aaagactatc cagtagaagg tatcagtgaa aaatatcgcg
     7981 gacgtgtttt gtctttctta ttgatgcatg tagtgacaca acgcatcgat gctcatgtgg
     8041 agcagttgaa ctgccatcca ttagagatac gccgctacta tcgtcagttt gattattagg
     8101 atagactgga taaaaagaat ataaaaaata aagagagcag cgataactgc tccctttatt
     8161 tttttaaaat attggcatca attacgttat aaaacacatt ttttgcatta tctacagttc
     8221 aacttataat ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta tgcaatagta
     8281 ctatttagtt gttgcatgga tccgtagtaa aagtccaagt acaacgagtt gcgccagttt
     8341 acatagcgtt ttgtatccag aatgttgact aaaacaaaag ttcctatgtc aaccgtcaac
     8401 agagacaatc tgtccgttgc aggacctgac agatttagaa atgttcaatc tgtctcttat
     8461 gtattttaaa tcaatactca ctaggagaag tttcctaacc tcctaatagt aatgctggta
     8521 gtaacattgt caaaactgct tcctcttagg aacaagcaaa gcataacgag tgtaaaaata
     8581 aaatgtagac tattgtaaca tatcattgat tatttcaagt aaaaacgata aaagaagtaa
     8641 acaaacactt aaaaacaatg gaaattagtc ccaactttaa gaaatgtata ctataatgga
     8701 aattatcaat tatttatttg gaggaatatc catgaaacaa aaaatcgcag ttcttggtcc
     8761 tggatcttgg ggcactgctt tagcacaaac attggcagaa aacgggcatg atgtccgtat
     8821 ttggggaaat gtacctgaac agattgatga aatcaataag tatcatacaa atcagcattt
     8881 tttacctgat ttgacgatcc cagaatcgat tatcggatat aaagaattgg cagaagcagt
     8941 ggataacgca gatgctattt tatttgttgt tccaacaaaa gcgattcgtt cagtcgcaca
     9001 ggaacttatt ggtaaaatga atactaagcc aatcattata catgcaagta aaggactaga
     9061 acaagatacg cataaacgaa tctcagaagt tttggctgaa gaaattccag aagaaaaaag
     9121 acaggcaatt gttgtattat ctggtcctag ccatgctgag gaagtggcag tacatgatat
     9181 cacaacaatt acagctgcaa gtattgatca aaaagcagca gcctatgtac aaaaactgtt
     9241 catgaatgat tatttcagga tttatacaaa taatgatgtg attggcgtag aaactggtgc
     9301 agcactgaaa aatatcattg caattggtgc cggcgctatc catggattag gattcggaga
     9361 tgatgcaaaa gcagcaatca tgactcgagg attggcagaa atcagccgtc ttggtgtcgc
     9421 gatgggtgcc aatcctctga catttatcgg tttgagtggt gtgggggact tgatcgttac
     9481 ttgtacaagt gttcattctc gcaattggcg tgccggtaac ttgttaggac agggacataa
     9541 gctggaagaa gttctagaaa acatgggaat ggtcgttgag ggtgtggcaa cgacaaaagc
     9601 agcagtagag ctagctcaac agctaggtgt tgagatgcca attactcaaa caatctataa
     9661 tgttttgtat aatggagaag atatcaaaaa agcagcaaaa gaaatcatgt tacgtgacgg
     9721 aaaaatggag aatgaatttt ctcttcgtta aatagatgat caatagttaa tcgt
//
LOCUS       DACDLI010000084         9616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDLI010000084 DACDLI010000000
VERSION     DACDLI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.084"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..244
                     /locus_tag="IV353_002303"
     CDS             5..244
                     /locus_tag="IV353_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010503.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            311..520
                     /locus_tag="IV353_002304"
     CDS             311..520
                     /locus_tag="IV353_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010504.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            580..1251
                     /locus_tag="IV353_002305"
     CDS             580..1251
                     /locus_tag="IV353_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9010505.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1304..3280
                     /locus_tag="IV353_002306"
     CDS             1304..3280
                     /locus_tag="IV353_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9010506.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGANSKYIKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEIVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGIGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGKYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGVLSAVVYFKKKA"
     gene            3295..4047
                     /locus_tag="IV353_002307"
     CDS             3295..4047
                     /locus_tag="IV353_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9010507.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4063..4824
                     /locus_tag="IV353_002308"
     CDS             4063..4824
                     /locus_tag="IV353_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010508.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            4837..5097
                     /locus_tag="IV353_002309"
     CDS             4837..5097
                     /locus_tag="IV353_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010509.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAVSMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5094..7184
                     /locus_tag="IV353_002310"
     CDS             5094..7184
                     /locus_tag="IV353_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9010510.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDFGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYTHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDNMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEVSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7211..7675
                     /locus_tag="IV353_002311"
     CDS             7211..7675
                     /locus_tag="IV353_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010511.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFIN
                     KPDSLFIVVPYEKDGVKKIVFLGSSKDEKRMKEKLIHDGSQSNKYKLQKTSE"
     gene            7672..7914
                     /locus_tag="IV353_002312"
     CDS             7672..7914
                     /locus_tag="IV353_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010512.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            7932..8234
                     /locus_tag="IV353_002313"
     CDS             7932..8234
                     /locus_tag="IV353_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010513.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            8306..>9616
                     /locus_tag="IV353_002314"
     CDS             8306..>9616
                     /locus_tag="IV353_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010514.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISK"
BASE COUNT         3594 a         1472 c         1988 g         2562 t
ORIGIN      
        1 gtagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa aaggaaggta
       61 tgtacagctg ttagctcgtg gttattcacc taaagaagta ttcaaagcct tatctaaagg
      121 aacagatatg gaaaaagaaa gattgcgaaa agaattcgat tattggagaa cccataatgg
      181 aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga taacccaaga
      241 ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa caaggaaggt
      301 aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga aagaaatgga
      361 agaaaagtta ctaaaaacga tgaagcaaaa acaccttaaa agattatctg tcgcgcaata
      421 tatcaatgat atgcagttaa caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa
      481 ctttgaacag ttgagacgaa cttatagaat tcatttttaa aactttttct atggaaatga
      541 gaaaaagttt ttttgttttg tccgaaagga ggcagatgga tgaaaaaatg gttatttggt
      601 tttttaggag tcactttaat cgtcttttgc agtgtgtttg gctatgttag ctaccaaaaa
      661 catgaaggag aggtgtttaa gcaaaatata gaaaaaaaga tgcccgtaga tcaaatcaat
      721 gcacatgcaa agagttataa ggaggatgca acgaacgtca ataatgatat gtctttgggg
      781 caaatgttgt ccatacaaaa agaagcaatc gaaatgggcg tcaataaaca agtatttgca
      841 caaattcaga ttcctgcact tggtttagca ttgccaattt ttaaaggggc aaatcaatac
      901 actttgagtc ttggagcggc tacttatttt tatgaagatg ccgaaatggg caaaggaaac
      961 tatgtcctag caggtcataa catggaaatg ccaggtgttt tattttcaga tattcaaaag
     1021 ttgtcattag gggaggtgat ggacttagtt agtaacgatg gcgtctatcg ttacaaggta
     1081 acgtgtaaat ttatcgtatc tgagtacttt aaattaattg atggcgttcc tgaagagaac
     1141 tctttcttgt ctttaccaaa gaaaggagag aaacccttat taacgttgtt tacttgtgtt
     1201 tatacgtcac aaggaaaaga aagatacgtg gtacagggag agctacaata acaaggttgt
     1261 aatcaacgcg agtcgttgaa atatgaaatg aggagagtaa aaaatggcaa taaatcggta
     1321 tgtaaaaaaa gctggaatgc ttgttggaat gttaggaatc attgcaggct gctttcaggg
     1381 atatcgagca tatgcagaag atgtcacgca aaaaacgcca ccagagaagg tcaatatcac
     1441 ggttcataaa ttaatgtacg atcaaggaac gcaattgaat gttgatattg acgggatcaa
     1501 aaacgatggt tatacgcacg atcaatatcc tacaggtgta acgaaataca acaaggcaga
     1561 ttatggtgat gttgagttta cgttaggaaa tattacggat caagtattac cgactgaaga
     1621 ttctgatcta accaacgcga aggtggacga aatcgttaaa gatgtagaag acaaaggtgc
     1681 taatagcaaa tacattaaaa atgcaaccaa caaaattact tcagctgtag atgaaaatgg
     1741 tgagatcact tttgcggatc aaccagccta tgtcaattcc aaagggaatg tatatgtggt
     1801 ttatgaaagt aaatccgctg ctggattagt cacacaaaaa gcaaaaccaa tggttgtgat
     1861 tgcgccaatg acagacaata caggatcagg atttttaaaa gatattcatt tgtatccaaa
     1921 gaacattgtt tctaagttga gttttgaatt aacgaaattt ggtgacgatg gcacagctca
     1981 aagcaaacaa acacctttaa aaggggcaaa atttgagtta tacaagggcg aacctggaaa
     2041 aggaacaaaa ttaggagatc ttgtatcgga tgatcaagga aagctgactg cgactgattt
     2101 gacgttagga aaatactatt ttgtggaagt tccttcagaa attgtagtgg gatcagataa
     2161 agaaccaaca gccgatcaat atttattagg cgcagacgct cgcaacgatg cgcacaataa
     2221 attaaccttt gaaattacga atgacggtgt gacaagcgat ttaaaagctt catacgtaaa
     2281 ctataaagca ccagtgatcg ataaaacagt aacaaatgga acaggacaag aacattcttt
     2341 ccaaattggt gacgcagtaa attatcaagg aacgattcat atcccaacag acattgcagg
     2401 cggagcagac ggtatcacag taaacggcgt gaaaagcgaa acttctcctt acagtgtatt
     2461 taagtggggg gatacagcag gacaaggact ttcttatgtc gctgctaaag caaatatcaa
     2521 agtaacaaat aaagatggat ctgtggtatt aaaagaaaat acagattata aaatccaaaa
     2581 ttcagaaaat ggctttgtga tcgattttat cgtgaataat ggccaagtaa gtgatactgt
     2641 agcaagttta cacggacaag atcttcaaat gacttataac atgtatgtaa atgatagcgc
     2701 agctgttgcc aatccattaa caaatagcgt ggatttcgtt tataacaaca atccatttaa
     2761 tcaggaagaa caccacgaaa aaacgaaagc tgacgttgtg acttatggtg caaaattctt
     2821 aaaagtcgat agcggactat ttggaatagg aatcaaagca acaccgcttg aaggcgcaga
     2881 atttgctgct aaaaacgcag aaggaaaata ttatggtgga ttagttgata cggataaaga
     2941 cggtgtcaaa gaagcagtat gggtagacga tgtagcaaat gcagcgatcc taaaatcaga
     3001 taaagaaggt cactttgaaa tcactggttt aactgaaggt aaatattcac tggaagaaac
     3061 aaaagcgcct gaaaactatc aaaaattaac gaaagagatc tctttcaaag tagacaaaga
     3121 ttcatacaaa gaagaaaacc gaatcacgat caaaaacaat caaaaagctt ccgtaccaat
     3181 gaccggtagc aacggttttc aaacgtatgt attaatctct tgcttgttgc taggagcagg
     3241 ggtactttct gcagttgttt actttaagaa gaaagcctag aaggatcaaa accaatgatt
     3301 atcaggaagc ctaagaaaaa gcgaatcatg ggtaaatgga tcattgcttt ctggttactt
     3361 tcagctgtag gtgtcttact tttgatgcct gctgaagcga gcgtggcgaa ataccaacaa
     3421 aatcagcaga ttgccgcaat cgatcgaaca ggaacggcag cagaaacgga ttcttctttg
     3481 gatgttgcta agattgagtt aggcgatcct gttggtattc ttacaattcc aagtatttct
     3541 ttaaaactac caatttatga cggaacgtcc gacaaaattt tagaaaatgg tgtaggtatt
     3601 acggaaggaa ctggtgatat tacaggagga aacggtaaaa atcctttaat cgcaggtcat
     3661 agtggacttt ataaagataa tctttttgat gatttacctt cagtaaaaaa gggagaaaag
     3721 ttttatatca aagttgatgg tgagcagcac gcttatcaaa tcgatcggat tgaggaagtg
     3781 caaaaagacg aactacaaag aaactttgtc acttacttag aaccaaatcc caatgaagat
     3841 cgagtaacgc taatgacatg tacgcctaag gggatcaaca cacaccgttt tctagtgtat
     3901 gggaagcgag taacctttac aaaatcagaa ttaaaagatg aagaaaataa gaaacaaaaa
     3961 ttatcttgga aatggctttt aggaagtact gtttttcttt cagtcatgat cattggttcg
     4021 ctatttgttt ataaaaagaa aaaataaagg agggtaacca acatgagaaa tagaaaatgg
     4081 ctatttgttt gtgcagccgc tcttttttct ttcactgcct acacacagtt gacgtttgca
     4141 gacagacaag aaagcacgga tagcacagaa caaacaatcc aaacgtcaga tagcagcacg
     4201 attcaaagca gcgatgaaac cgtcgattcg tcaacagttg attcttcaga agaacctaaa
     4261 attgctgaag acgatcaaat agtcaaacag accattcacg ttcaaaaaat tatttcttcc
     4321 gaacaattag acaaccaggg gcaaaagctt gaaaaacaag aaggagtaaa tggcgcgaag
     4381 ttttctgttt atgatgtatc ggacatttta caaaagatgg atgttaagga tctcactact
     4441 gaccaaattg aatcccaatt aaaagatcga gtgaaaaaac tatccagtga tcaactgaag
     4501 ctaatttcta atggagaaac aaagacaatt gatcaacaaa caggtgtctt tgaattctcg
     4561 gtagaagtac aggcaaatca gaaacaagct tattatattg tcaatgaatc aagtcctgaa
     4621 aacatttcta atagcgagga tattttgcta ttaacacctg tttctgataa aaatggtgaa
     4681 ttcttaaagg atgtgtggat ttatcctaaa tcagaagctt cgcaacctaa agaagaagta
     4741 aaaaaaatcg tatctactgg cgtaaagaaa aacttctttg agaattgttg ggattttgtt
     4801 actcacttgt tttctagaga ttaaggaaga aataagatga aactgatcaa attattatta
     4861 ttcaatcttt cttgtttaac cgcagtcttt ttgtactgcg gtttattatt ttctcaacta
     4921 tctattttgc caagctacca actataccta atgaggattt taaacatacc agctgtttcc
     4981 atgccgctag atattatgga aaagctcatt aggaatccat tatttactac gatcaatttg
     5041 atcgttattg tcggaatttc actagtcatt tataaaaaag aggggatgaa gggatgaatt
     5101 ttaaacactc gtggacaaga cggttaaaat atgtttttcc cataatgatg atgttaggta
     5161 cattgtttgg gttaaaaaca gccaatgtct ttgctgaaaa agttattgtc gatccgtcaa
     5221 atccaataca gaacgtaaag aataatttta ttattccaaa agatgttcct aatgcaaaaa
     5281 caaatattac tgtaaacgga gcgtcaaaag tttacaaata tcatgttgat gccaacaaag
     5341 gtggttttac agacgactac gtaggattag tggagggaaa caaaaatata cgctatcctt
     5401 ctttccaaaa ggaatacggt gaaacaaacc ttgttttaga aggcgtaaca accaacacca
     5461 aagtcaatat tgactttgga aaaatcggta cgtataacgg gaaaaaagtc aatatcaagc
     5521 tagttttatc taatattcac ttatactcgg atacgttgcc gtggaatcta ctagataata
     5581 actatacaaa aactcatttt cgagacgatg gctataaaaa tacgaatggt gcaatgagta
     5641 aatctaaaaa aagaacagta ctttggatct cagacaatct tttctcagga attgtttacc
     5701 attcaacaca aatgaatgtg cagctagtag ccacatatga ggacggtagc cccgttcaat
     5761 tttcaggaga tacatttatc agttttaact ctttgaaccc tgcaggagga aaaagcacgg
     5821 atcttaaagg agaatatacc cactatgaca aaatgaatac aacagattgg tatgtcagaa
     5881 aagatacggt tctttcagaa tttaaatctt tctacaacaa tctgaacgtg gtaggtggcc
     5941 acccaggagg aagctctaag ctgacacaag cggataatga tttcaataat ttgcatgata
     6001 aattaggaga tccgaagttt ggtcaaggta ctgtgtcctt taaaatatct gaagcaaatc
     6061 cgacgtttgt gattggttct tctaatgttc aaacctggtt tactttatct tctgcgacaa
     6121 tctttagtgt tgtacctgat caacctgaaa aaacaggggt tgataaaaat ggaaataacg
     6181 tgaacgataa catgttgcaa gtaggagaca cgattcaata tcgaatcaaa cagaaggtga
     6241 atcgattagg agtggattta ctagcggttt acgatcgatt tgagttaatc gataatttac
     6301 caaaagaagt agattacgtc gatgcacacg ttgaaagtgg tacaaataaa aaattcgatg
     6361 tatctggtga agtgacctat gacaaaacaa aacaccaggt aaaatatgca gcgaaagctg
     6421 atacattaaa aaatcgaatg aagtataacg gcgaaactta cgaattagtg atcaatgtaa
     6481 aagtcaatga attggccaat caaaatagtg tagccaaaaa tcagggaacg tcgatcatta
     6541 ataaagtaga aaaggataca aatatcatta ccgtttattt ccctaaaatt ccagtgaaag
     6601 aagtacaaca aaatggaaaa gatgtaaacg gacgaaatga cggtaagaag ggtacgccga
     6661 cagctccttt aaacgcagga tctgaagtac aatatctagt cacacaaaaa tggcacacaa
     6721 aaggcgtaga cgctgcgtca gatcactaca aacaatttag tattcaagat ccaattgaag
     6781 ctcgtttgac atataaagaa gtttctgcgc aagtaataga taagagtaaa ggaaaagata
     6841 ttacttctga aggcacacta acctatgata gcaattcaag aacgctgaag tgggaagctt
     6901 ccgctgactt cttaagtaac aatcttttag atggacgaga aattcagctg atatttacag
     6961 ctaaaactcc attacaatca gaaaaaaata ttgataacca agccgtagta gcagtagata
     7021 atgtttctaa taaaacaaat gttgtgacaa ttggggttga tcctaacttg ccacaggtca
     7081 ttgttcctaa aacaggttct acacatttag taacgatttc agcggtcagc ttactattac
     7141 ttgtgttagc aacttttagt tatgcggttc tgagatacat ttaaaataaa aaaacgaaag
     7201 aatgaataaa atgaaaaagt taataatagt agttacttcc ttgattgcca tgattgttat
     7261 tttagtcgga tgtagtaatg aaaaaaataa acaaactaat caagataatg gcgtattgaa
     7321 atcaggaact atgtggaaag aagaagtagg tggtcttgtt tataatttga aaattataga
     7381 cgagactacc tgggaatatt ctgaaagtgt atggcatcca gatcctgtac aaataacagt
     7441 taagagacaa aaagactatg aagggttaga aagatacaaa atagtagatt ctgcaggtat
     7501 cagaaaattt ataaacaaac ccgattcttt atttatcgtt gttccttacg agaaagatgg
     7561 cgtaaaaaaa attgtttttc ttggatctag taaggatgaa aagcgaatga aagagaaatt
     7621 gattcacgat ggatcacaat ccaataaata taagttacaa aagactagcg agtgattgct
     7681 agtcttttta tttgtcctga aggaggaatc acagtggcaa aagctaaaac gccatttgaa
     7741 aaagccgcgt ctaaaatctt agaacgccgc ggtgtatctg aaacacagtg gaaacaagaa
     7801 gtccttgaaa aagcacaatt tgacctatta cggggaaaag acaaggactt agaaggatat
     7861 atctatcaac gtgaacgtga aaagcttgtt ttcgatgaaa ttattaaaca ctaaaaggag
     7921 cgaacaattt tatgtttgaa tcaattacaa tgaatttttt aacacaaccg aaagtagtag
     7981 ctgcggttga tatttttaga gcattacaaa ctgccgcaac tagtttgaaa aataatctga
     8041 tgatttttgc tgctgttgcg gcagtggctt ccattgctct tggaggtatt ttcttcttct
     8101 ttgggcgaag aggagctgaa aacgggaaga cacacgtagg ttatgtattg ttgggggtag
     8161 gagttatttt agctgccgct tctattgtag tttcaatgtt ctcaatgttt ggacaaaccc
     8221 cacaaggact ataaaaaaaa caagtagaag gagtaaaaag tacgtggcta ctttttactc
     8281 ctttttttat gaaaggagaa tattcatgtt ttttacgatt tcaggaaatg caaaattgga
     8341 taaagagtta ataagaaaag caacgaagaa agtgggaaag cgtacttcct ttttaccgaa
     8401 aacaattaaa tattctgtca ttgaaacgga agaacagcta catgtgttag gacaagtgtt
     8461 agaacaagag aatcaatctt ctgttaaatt gactctcacg ttacatgata aagaaggaaa
     8521 aatagaaaag gctggcacag aatacgtttt tggcccactg gattatcaaa tttcagaagg
     8581 tttttatcct acattgatgg atttaatccg ccttgatgtg tttaaagata cggatgaaac
     8641 tatgagtgtg gaagagtctt atcgaaacga aaaactttta caagttttag aagaagagtt
     8701 agagagcgac caaagaaaag tgaatcaagt gagtgaagtc gaagtaaatg aagaagtacc
     8761 atgggaacga ccaatatttc caaagaagga gtcaaaagaa aaagagacag tctatgttga
     8821 cgaacaagct gatattccga ctcagaaatc gatatttgag gaagagaacg atgtgatggg
     8881 tcaattggag gtagatccct ttgaagaaac aatggaggat gaagagacgg acgaagtaga
     8941 agttgatcca gttgaagcta atgaagagtc aacatggact gaaggggatt ctcaggaata
     9001 tattgaacca cttatcagtg aagaagtaga aaaaaacgaa acagagatac aaaaggcaac
     9061 tgtatttgat tatattttgg atcaatatcc gccagagcat gaatggatga aagaaaaaaa
     9121 caaattattt ttaaatgagc tttattcgaa atatcaatta ccccaaacct atcaagcatt
     9181 tttagagagt aaagaagaat taatctcaca gggtaaaggc acacttttag aacagttaga
     9241 gtctgtccaa tcaacaaatt ggcatgagct tgcacaagaa gatttattag ccacctttga
     9301 agaacgtcaa caagaaacag atcaagaaat taatgagtat gcgacggaac agtcgtctaa
     9361 ttgggagaaa gtaaaagagg aactaaataa agaagaacag cgagtgattg aagaagaagt
     9421 acaaaaaatc aaagagcggt acgaaaagaa acgcgacaat gcttattcct tgtgtgtaga
     9481 aaagatcaac aattttacta agacgaatca agaagcgtta tcgaaagaaa aagaaaactt
     9541 gttaaaagaa cgtattaaag aacgaaaaga agagttctac cgcggtttac gtacagataa
     9601 gctaaagatt agcaaa
//
LOCUS       DACDLI010000085         9609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDLI010000085 DACDLI010000000
VERSION     DACDLI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.085"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..587
                     /locus_tag="IV353_002315"
     CDS             <1..587
                     /locus_tag="IV353_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9010515.1"
                     /translation="IANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGV
                     DLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSR
                     DGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAK
                     NNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            787..1101
                     /locus_tag="IV353_002316"
     CDS             787..1101
                     /locus_tag="IV353_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9010516.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1122..1499
                     /locus_tag="IV353_002317"
     CDS             1122..1499
                     /locus_tag="IV353_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9010517.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1509..2282
                     /locus_tag="IV353_002318"
     CDS             1509..2282
                     /locus_tag="IV353_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010518.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2304..3707
                     /locus_tag="IV353_002319"
     CDS             2304..3707
                     /locus_tag="IV353_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9010519.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3889..5073
                     /locus_tag="IV353_002320"
     CDS             3889..5073
                     /locus_tag="IV353_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9010520.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5070..5360
                     /locus_tag="IV353_002321"
     CDS             5070..5360
                     /locus_tag="IV353_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010521.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5357..5578
                     /locus_tag="IV353_002322"
     CDS             5357..5578
                     /locus_tag="IV353_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010522.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5620..6399
                     /locus_tag="IV353_002323"
     CDS             5620..6399
                     /locus_tag="IV353_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9010523.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6466..6960
                     /locus_tag="IV353_002324"
     CDS             6466..6960
                     /locus_tag="IV353_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9010524.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            7016..7654
                     /locus_tag="IV353_002325"
     CDS             7016..7654
                     /locus_tag="IV353_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010525.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7726..8121
                     /locus_tag="IV353_002326"
     CDS             7726..8121
                     /locus_tag="IV353_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9010526.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8105..>9609
                     /locus_tag="IV353_002327"
     CDS             8105..>9609
                     /locus_tag="IV353_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP9010527.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKA"
BASE COUNT         3325 a         1538 c         1861 g         2885 t
ORIGIN      
        1 taattgcaaa cgatatttta attatattaa aaaatgaatt tggaatagaa gttaaagatt
       61 attttattga cttagattta ttacagctaa taaacagtgt gaaaagaatg tatagtggag
      121 acgacaacgg tgttgattta tacaaatttg ttaccaaaca tatatttgtt aatcttaata
      181 ttgacgagaa aaattacccg tctattttta agcactatct attttttatt aacgaagtag
      241 aagatcggat aaaaaataat tcattatcta gtagtgtatc tagttttaag aagatgttta
      301 aatctcgaga tggtatcaca cttactacag cacataaagt taaaggaaat gagtatgata
      361 ctgttatcgc tattaatctt ctaaacggga aaattcctca ttggaatgaa atttttaatg
      421 tttctgataa aggaattagt tcagcgagaa aactactata cgtaatttgt tcaagagcaa
      481 aaaataattt atttcttttc tcagaggtgg gttattttac gagtaaagga gacttgcttt
      541 atcccactca attattagat aaaatagaat ttgattataa tatttaaaat acctaggtag
      601 tcgttgtttt aaatatagta ggaatcggac gctcagtacg gagcgtctat ttttttaccc
      661 aattttgaaa ggaagtgaac ttatgaaaat aaaaaatcaa gaatcaaaag gtcgctctcc
      721 cccctttaaa accatcacac aaataagatt cattcaaaca taaaaacaga aaggataggt
      781 aaaaatatgg aacttaaata tgtcattccc aatatggaaa aaacatttgg acaattagaa
      841 tatgctggtg aagggaacat tgaacaacgt cgaataaatg gcagaccaac cattctctct
      901 cgtagttaca atttatactc aacgattcaa cgagcagatg atattgttgt cattcttccc
      961 tccgaagctg gcgagaaaca ttttgaatcc gacgaaaaag tcaaactcat caacccacat
     1021 attaccgcag aaggttataa aattggcaat cgaggattta ccaattacat tatgcacgca
     1081 gacgatatgg tgaaagcata agaaaggaaa ggtgaaaaaa tatgagatta gcagaaggta
     1141 ttgtgattga aaaagaccag acatttggca cattgaaatt ttccgccttg cgtcgtgagg
     1201 ttcgtatcca gaatgaagat ggtacggttt ccgaagaaat taaagaacgt acctatgact
     1261 taaaatcaaa aggacaagga cgcatgatac aagtcagtat ccccgcttct gttcctttga
     1321 aagaatttga ctacaacgca gaagtggaat taatcaatcc tgtggcagat accgtagcga
     1381 cggctacttt tcgaggggca gacgttgatt ggtacgtgaa agcagacgat cttgtattaa
     1441 aaggacagac aaaacctcat cagaaatata aaaaagaagc gtccgaaagt gaaaaatagg
     1501 tgttgcttat gacaaacaca ccattgattt tgaaagaaat tccgaaggat gaagccattt
     1561 cttttattcg gcaataccat tattcaaaga tactccctag attgtgtaaa tactttctag
     1621 ggattttttc agaagaaaaa ttattaggcg ttgtggaatt aggctggggt acgcaacccc
     1681 tacaaaccat acgcaaactc tttcccgaca gttcccttca aaccacagat tatttagaaa
     1741 ttggaaagat gtgttttctt ccagaaatga atcaaaccaa ttattttggc agtcaagctc
     1801 tttcagctct aattaagtgg ttgaaagaac atacggactg tcatttttta tatacactgg
     1861 cagatggaat tgagggaaaa tgtggctatg tctatcaagc gtccaacttc ttttactgcg
     1921 ggtatttcaa gacaagtgtc tatcgtgata agcagtcatg ggaaaagata caccctcgaa
     1981 gtgctcgcct tttattagag gaaaatgctc gctttgaaca ggtagagaaa aagcattggc
     2041 tttcccaggc attttgcgaa tacaaaggga ttgagaaaat caatggacgc atgtttcgct
     2101 atctctatcc gttgacaaaa gaagcgaaaa aactgttagg acacactctc tatcgacgtc
     2161 attattatcc gaaagaaaaa aatttacgct ttgaaaagcg aatcgcttat cagaaatatg
     2221 aagccatttc tcaaccaact tttgataaac aggctcgtat ctacaataca caattatttt
     2281 gataggtgga aaggaggatt tttatgccat tgcttcatga cagaggagag cgaatcaaag
     2341 ccaacgataa gcacttggtc tatcattttg tactaatttc attgctggta gtttttctag
     2401 tcgtcctact cccttttcat tggagagcgt tacaatcggt gaactggcaa gcagttcagt
     2461 tagatacatt tttctatcaa cttcatacgc catacttttg gagcagtatt ctcactgctc
     2521 tttttgtatg cggacttatc agctggcttt attatcacta tcgtatagat tctataaaac
     2581 agttagagca ccgtcaaaag ttagctcgta tgattttaga aaatcattgg tatgaagccg
     2641 aagaaactca atcagaggga ttctttaaag atttacccag caagaaaacc aaacaacgaa
     2701 tccgccattt tcctaaaata tattatcgaa tgaaagatgg actaatccat attcatgtag
     2761 aaatcacctt agggaaatat caagatcagc ttttgcacct agaaaacaaa ctagaaacag
     2821 gattgtattg tgaattagtg gaaaaaacgt tgaaagattc ttatgtagag tatgtcctac
     2881 tttatgatac tattggcaat cgaattggca ttgaagacgt ggtagctcaa gatggacgtg
     2941 tcaaattgat ggaatcggtc tattgggagt tcgattctct ccctcacatg ttaattgctg
     3001 ggggcactgg tagtggaaaa tcctatttta ttctcacatt gattgaatct ctcctgcata
     3061 caaatgcaaa gctttatatt cttgacccta aaaatgctga tttggcagac ttaggggcgg
     3121 tcatgaataa tgtctattac cgtaaagaag atatgttggc atgtatcaat caattttacg
     3181 aagatatgat tgatcgcagt gaaaccatga aacaacaccc taactataaa acaggagaaa
     3241 attatgctta tcttggtttg tctgccaatt ttcttatttt tgatgaatat gtggcaatga
     3301 tggatatgct aggtcgtgaa agtaccaccg ttattcataa actcaaacaa attgtaatgt
     3361 taggacgaca agcaggcttc tttttaattc ttgcctgtca acgtccagac gcaaaatatt
     3421 ttagtgatgg gattcgggac aactttaact tccgtgtggc tttgggtcgt atgtctgaaa
     3481 tgggctttgg tatgatgttc ggaagcgata cacaaaaaca atttttctta aaacccatca
     3541 agggaagagg ttacgtggat acaggaaaaa gtgtgattag tgagttttat acaccccttg
     3601 taccaaaaag gtacgatttc ttaggagaaa taggaaaagt cattcaaaaa aagcaatctg
     3661 agcctgtacc gcacgaagtg aaaggtacag gttcagactt gctttgattg gtgtggcgtt
     3721 agccacgcca atcctttagc cccctctatc taacaggggg gcacaaatac aactgaaaac
     3781 aggcaattct catcaaccaa tgttgatggt acaaaggatt gcggaaaata tgaaaggtgt
     3841 aaactcaagg gtcatagttt acatcttttt tagattggag gtttgcgaat ggaaaacgaa
     3901 aaaatttgga ttaaggaatt gaaagaaaag cgactggctt atggcgtttc tcaaaataaa
     3961 ctggcggttg cttctcatat taccagacct tacctatcgg atattgaaac aggcaaggca
     4021 gtccctactc aacaggtgaa agaagattta ctgaacgctt tggaacgctt caatcccgat
     4081 aacccattgg agatgttatt cgactatgta cggattcgat tccctaccaa tgatgtggct
     4141 caaattatcg gtgaggtttt acgtttgaat atggattata tgctacacga agactttggc
     4201 tattattctt atcccgaaca ctatcgtttc ggtgatattg ttgtcctagt ctctcatgat
     4261 gtaagtaaag gtgttttatt agaactgaaa ggaaaaggct gtcggcaatt tgaaaacttt
     4321 ctattggctc aacatcggag ctggtatgat ttctttcaag attgcatgga acataaaggt
     4381 attttcaaac gcttggattt agctatcaac gataaaacag ggattttgaa tattccagat
     4441 ttggcaaaga aatgtaaaca ggaagaatgc atttccgtct ttcgaagctt taaaaattat
     4501 cgcagtggtg aattggttca ccgtgatgaa aaaccataca tgggaaacac actttatatt
     4561 ggctcattaa aaagtgaagt ctatttctgt atatatgaga aagattatga gcaatacgtg
     4621 aaaaatgatg ttcccttaga agacgcagaa gtaaagaatc ggtttgaaat tcggttaaaa
     4681 aatgaccgag ccacccaagc catgaaagat ttattggcac atcaacaggc ggaaaaaaca
     4741 gcctttgaaa ttatcaatcg ctatatccgc tttgcagata aagacgacac taaacgtcgg
     4801 agtgattgga aaactaatga acgttgggaa tggtttatcg gtaaaaatcg tggagcatta
     4861 cgcctaacca cacagccaga gccttattct tttgaacgaa cattgaactg gttacatcat
     4921 caagtcgccc ctacattaaa aatcgcaagc attttagatg ttctcaacgg tacaaccatt
     4981 atctccacca tgattcagga agccaaactg acagaaaaac atgagaaatt aattgaacaa
     5041 caacatctag ccatggagga tttgattaca tgagcctttt aacaaaagaa ttaaagaagt
     5101 tagggtttca gtgtggcatt gagtttcaag cgtatataca aaatacaggg aaatatacgt
     5161 ctctcattat tgagggaaaa cggcaagctg gtgataccat ctatacctat gatttttaca
     5221 aggtgagatt ttataataat tataccaatc gagtaaccgt ctatggagag catttaaccc
     5281 cttttcaatt attaaggcgt gtaaaaagct atatctatta tcgagaaaaa tatcttaagg
     5341 aaaggaggac aatcacatga attttgggca gaatttgtac aactggttct tggataatgc
     5401 acagtcattg gtattgttag cgattgttgt cattggactg tttttaggtt ttaaacgaga
     5461 attttcaaaa ctcattggct tcttgattat tgctctaatc gccgtcggac ttgtgttcaa
     5521 tgcagctggc gtgaaagatg tgttattaaa cttatttaac cgaatcattg gtgcttaaca
     5581 gatatttttc atgataaaat aggaagtata tattttgtta tgaaaggatt tatgaatatg
     5641 tttacaaatg atcaatttaa atctctttta attaaaacat tagaaagaaa gagctcttca
     5701 aattattatg aaggaggtaa cgagattgtt tctaaaatga aaatatcaga ggtaacttta
     5761 gatgagactg acgattttac ttattataaa ggatataaaa agggctggaa tacgctttgc
     5821 acatatctta aaataagagt tccttttgat gatttagact tttttgaaag tcataaagat
     5881 ttgattaccc agacagcaag ctccatatat gataaacaag gtgataatgt attagtggat
     5941 actattttag taccacttcc agagaattac gaagtaatta actttagtca gttgaaaatt
     6001 agtgatgtgg tttcacaagc tattgaagac gctgagtctt ttatgagtaa tggtgaatat
     6061 caacgtgcct ttgacagagt tcacactgct tttcatggat atttaattga aatactaaaa
     6121 aaatatgaaa ttacagttcc tagagacgaa aatctatcta aattatatag taggatacaa
     6181 caattaattg aaaaagaaat tcaacctact gaacttgctg atatagtgaa gacaacgata
     6241 aggtcatcaa atggaatgat tagctctctt aatgaagcaa gaaataggca ttcacttgca
     6301 caccctaata caaatattat tggaaagaga gaagcaaaac taataatagg tatttcaagt
     6361 acagttacag attatattag cggatacctt gataagtagc gtcattaact gattgctact
     6421 ttttttatga ctatttttat aaaaaggatg tgataagaat tggaaatgaa agtctatgtg
     6481 gcaaatttag gacggtataa tgaaggtgaa ttagtcggag catggtttac tccaccaatt
     6541 gatgaggaag aaatggcaga aagaatcggt ttaaatgagg actacgaaga atatgcaata
     6601 catgattttg agcttccttt tgatgtggac gaatacacgc cgattagtga aatcaaccgt
     6661 ctatgtgaag ccattcaaga aatcgaaggt acacctattt ataatgaact caaagaaatt
     6721 caaggcatgt ggtttagtag cttggaagaa ttactggaaa acaaagagga tattcattgt
     6781 tatagtgatt gtgattcgat ggaagatgtc gcccgttatt acgtggaaga aacaggacag
     6841 cttggtgaag tcccttccaa tttacaaaat tatattgact accaatcttt gggacgtgat
     6901 atggaaatag agggtaatta cctagttacc tctcatgggg tatttgaata ttgtcagtaa
     6961 gtttaacaat ccgtgttata attaaattaa taaatagcac ggagaggtat aaattatggt
     7021 aaatgtattt gaaaatctta aactattaaa actagacatg aaagataaag gctgggtgat
     7081 agattccttt tactttcgtt acaaacagca aaattatatt gtacttgtta agctatttga
     7141 aaaagaggaa aaagttccag aatatgctct gttaaaacta gaatttttaa aggaaaacga
     7201 tttttcagat atgctagctg tctatgctaa ttcggtaaaa ttatatacag acacgaaaac
     7261 aataagagaa tattttggta tcgaatacag tagtaacttg ggagatgttt tatttcaatt
     7321 tagccaaact cttgctcgat ttattccgac agaagttagt gagaagaaaa atgaagacca
     7381 aaaagaagcc atgtgtttct cattaagtca aagtgattca gaagatccca gaaaaaaata
     7441 ttgtttttca gttagaagaa atcctttaaa gggaaatggt gagctgggaa aacgaagtcc
     7501 ttttaatgac aataaaacga gattgtatag acccagctta tatgaacgat tagggtcaga
     7561 taccaattta agtttcagat attcaatgaa tccagatgat gaagaaactg atgaaggtat
     7621 tattgcaaaa tggacaaaaa ataaaattga atagttaaag cattgaaaga gcaatcacct
     7681 attttgattg ttctttttct atttttacgg aaaggacgtg aagacatgaa aaaaataaga
     7741 agttacacca gtatttggtc agtagaaaaa gtgatttatg cgattaatga tttacaactc
     7801 cctttcccaa ttacctttac gcaaatgaca tggtttatcg tttctttgtt tgtcgtcata
     7861 ctctttggca gtttaccacc actttctctg attgacggag cactactcaa atacttaggg
     7921 attcccattg ttctcactgg gttcatgtgc caaaagacct ttgacggaaa gaaaccctat
     7981 ggatttctaa agtctatcat tacctatgct ctacgcccta aagtaaccta cgctggcaaa
     8041 caaatacgat taaaaaaaga aacaatgaat gaatcgatta cgattgtaag gagtgaacaa
     8101 ctagatggca tatccgatta aatatatgga agataatctc gtatttaatc atgatggcga
     8161 agtattcgct tattatgaat taattcccta taattacagt tttttaagtt ccgatgaaaa
     8221 aattcaagtt catgataatt tctgtcaatt gattgcccaa aatcgtgatg gcaagattca
     8281 tgctctgcaa ctagcaaccg aatcaagtat tcgagccaca caggaacaat cgaaaaaaga
     8341 aatcactgga cgattaaaag agatagctta cgaaaaaata gatgaacaga ccgaagcgtt
     8401 aatttctatg attggtgaca atcaagtaga ctatcgcttt tttattggtt ttaaactcta
     8461 cctcaatgaa caggaagtat ccgtaaaaaa tgttgggaaa gaaattcaaa aagcatttgc
     8521 ggaattcttt catggtgtga atcacaaatt aatgggcgat tttgtttcta tgcctatggc
     8581 ggaaatcgaa cgcttttcta agatggaaca gttgttagaa agtaaattat ccagacgctt
     8641 tcagattcgt aaattggaga aagatgattt tggttatatt attgaacatc tttacgggca
     8701 aacaggtgtc ccttttgatg actacgacta ccatttaccg aaacgatacg aagataaaaa
     8761 agtgttggta aagaaatacg atattctcaa acccactcgc tgtttaatcg aagaaaatca
     8821 acgctattta cggattgaac aggaagatac cactacttat gtggcgtatt ttacaattaa
     8881 tagtattgtt ggtgaattgg atttccctaa tagtgaaatt ttttattacc agcaacagca
     8941 gtttactttc cccgttgata ccagtatgaa tgttgaaatt gtcgccaata aaaaagcctt
     9001 atctaccgtt cgcaataaaa agaaagaact gaaagattta gatagccacg catgggaaag
     9061 cgacaacgaa accagtacta atgtggtgga cgctttagaa tctgtcgatg aattagaaag
     9121 taatttagac caaaccaaag aatctatgta caaactaagc tatgtggtac gggtatcagc
     9181 aaagaatttg gacgagctga aacaacgttg tgatgaagta aaagattttt atgatgatct
     9241 gtctatcaaa ttagtacgac cttttggcga tatgctaggc ttgcataatg aatttattcc
     9301 ctcaagcaag cgatatatca atgactatat tcaatatgtc acgtcggatt ttttggctgg
     9361 tcttggtttc ggtgctacac aaatgattgg tgaaaattct ggtatttacg tcggttacaa
     9421 tctcgataca ggcagaaata tttatctcca acctagcctt gcaagtcaag gaattaaagg
     9481 ctctgtaaca aatgcattag cgtccgcttt tattggttca ttaggtggcg gaaaatcatt
     9541 ctctaacaat atgctggtct attattcagt actctttggc ggacaggcaa ccattgttga
     9601 cccaaaagc
//
LOCUS       DACDLI010000086         9126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDLI010000086 DACDLI010000000
VERSION     DACDLI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.086"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(101..364)
                     /locus_tag="IV353_002328"
     CDS             complement(101..364)
                     /locus_tag="IV353_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9010528.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(463..1758)
                     /locus_tag="IV353_002329"
     CDS             complement(463..1758)
                     /locus_tag="IV353_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9010529.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(1755..3026)
                     /locus_tag="IV353_002330"
     CDS             complement(1755..3026)
                     /locus_tag="IV353_002330"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9010530.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(3191..4060)
                     /locus_tag="IV353_002331"
     CDS             complement(3191..4060)
                     /locus_tag="IV353_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9010531.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(4352..5962)
                     /locus_tag="IV353_002332"
     CDS             complement(4352..5962)
                     /locus_tag="IV353_002332"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9010532.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(7155..8495)
                     /gene="glnA"
                     /locus_tag="IV353_002333"
     CDS             complement(7155..8495)
                     /gene="glnA"
                     /locus_tag="IV353_002333"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9010533.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(8523..8915)
                     /locus_tag="IV353_002334"
     CDS             complement(8523..8915)
                     /locus_tag="IV353_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9010534.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         2566 a         1968 c         1565 g         3027 t
ORIGIN      
        1 tttttcagca tcaaacgaag ttttaaattg gcaaaaaaca taaaaaaaca gactatccct
       61 atcagatagt ctgctaaacg ttgagttatc agcgttttta ttattccgca gcgtttgcgt
      121 ctttgagacc gtattttttg ttgaaacggt ccacacgtcc gtctgcttgt gtgaattttt
      181 gacgtccagt gtagaatgga tgtgagtcag aagttacttc gacacggatg actgggtatg
      241 tattaccgtc ttcccattca actgtttctt gtgaagtttt tgttgaacct gacaagaatt
      301 tgaaaccagt tgttgaatcc atgaatacta ctgggtggta atctggatgg atgttttctt
      361 tcatgatttt ttcgctcctt tgccctgctc catttgctga accagagttg atttgtcgat
      421 ttacaacatt aacatcttaa catgtttcag tcaaggttgc aattatcttt tcagatttct
      481 ggtcggattt tttttaccaa aggagacatc ttgaaattct ttgaaaaatt gctgattgtt
      541 ttttgtttta cgcaaaaagc ggatcagttg ttccgtatat tctaatgaat ctccagtcat
      601 attttttcga agtttccaaa tttcttctaa ttcttcactt ggcatcaata attcttcttt
      661 tcgggtacct gattttttga tatcgatggc tgggaagatc cgacgttcag ataattcacg
      721 tgaaagttgt aattctagat ttccagttcc tttgaattct tcatagatca cgtcatccat
      781 acggcttcct gtatcgacta aagcggtcgc taaaatcgtc aaactgccac cttcttcgat
      841 gtttcttgct gccccgaaaa atttcttagg tttatacaat gcggctgggt caatcccgcc
      901 actcaatgta cgtccgcttg gtggaacgac taagttatag gcccgagcaa gtctagtaat
      961 actatccatc aagataacga catctcgttt atcttcgact aaacgcattg cgcgttccaa
     1021 tactaattca gaaactcgag tatggttttg cggctgcaga tcaaatgtag aagataccac
     1081 gtcgcctttc acgcttcgtt ctagatccgt tacttcttcc ggtcgttcat caatcagtag
     1141 aacgattaat tctacttccg gatagttttc ggtgatacca tttgcgattt cttttaatac
     1201 agaagttttc cctgcttttg gcggtgcgac gatcaatcca cgctgaccaa aaccgattgg
     1261 agaaaagata tcgatcattc gggtagatag tctttgcggt gttgtttcca gttgaagctg
     1321 tctttgcgga taaagaggtg ttaaggctgg aaaatgcgga cgttcttttg cttcttccgg
     1381 atcttttcca ttaacgcttt ccacgtgcat caaaccataa taacgttccg attctttagg
     1441 tggacgggct tttcctgcca ctttgtcccc atttctcaaa ctgaatcgtc tgatttgtga
     1501 ggcagaaata tagatatctt ctgcacttgg tccataattg attggccgta aaaaaccgta
     1561 accatcttga ccaacgatat ccaatattcc ttccatataa taaaatcctt gtttctctgc
     1621 ttgtgcacga atcacagcta gagataattc ctttttattc atcttactgt aataaggaat
     1681 tttaaattct ttggcataac tataaacttc ttttaacgtt ttgttctcta gttctgccat
     1741 tgtcaaataa tcgctcattc agctcccact tcctctatca tttcaattga tgcccctaag
     1801 gctgttagtt tctcgataat atgatcataa ccgcgtaaga tataatctac attgtaaatc
     1861 gtcgttgtcc cttctgccaa tagtccagca atcactaaac aagcacctgc ccgaagatca
     1921 gaagccacga cttctgtgcc gtgaagttta tttggaccat tcacgatgat catatttcct
     1981 tccacggtaa tatcagcgcc cattcgtgct agttccggca catggttcac acgtttcgca
     2041 tagatcgtat cgatgatttc tgaagtgcct gtagtcatca atagtaacgc agtaagtggc
     2101 tgctgcagat ctgtggcaaa tcctggataa ggataggtca tgatgtttgc gggttttaat
     2161 tctttcgagg ggtaaacttc gatttcatct tcgctgattt tcatatggac accaatttcc
     2221 tgcaattttg ctataaagct ttccaaatgt tcaaagatca catttttcac tttgatacca
     2281 tttcctactg cagctgctaa tgctagatag gttcctgctt cgatgcggtc aggaatcatg
     2341 aaatgccgac agccatgtaa tgtttcgact ccttcgatac ggatcacatc tgtacccgct
     2401 cctcgaactt ttgcccccat attattcagt aaagttgcca catctatgat ttccggttct
     2461 cgtgcagcgt tttcaattac tgtctgtcct tttgctttta cagcagctaa catcacattg
     2521 atcgttgctc cgactgaaac cacgtccatg aaaatccgat tgccacgcaa tcctttattt
     2581 tctgtccgta aatacatggc accatgttca tttgtcactt cagcacctag agcttcaaat
     2641 cctttgacgt gtaggtcaat cggtctaggg cctaaataac agccacctgg caggcctact
     2701 accgcttcgc caaatttacc aagcagtgtt cccataaaat aataagaagc acgcaaacta
     2761 ttgatttttc cgtgcggcat cggaatcgat acgacttctc gaggatcgat ttctagtatg
     2821 ttgttggaaa aatggacagt tgcgcccatg atctctaaaa tttcaattaa tgaatgaaca
     2881 tcttgtatgt ccggcacacc ttccaagatc actggggaat cagctaagat tgcagctgga
     2941 atcaatgcga ctgcactatt ttttgcaccg ctgatcgtta cttcaccagc taaaggtcgg
     3001 tttcctttga ttacgatttt tttcatctgt ttccaccttg tttattaatc ttttttcatg
     3061 gatatgcttg tattttatca tagataaaaa aaagaaacga gcatggtctg ccgttttaag
     3121 taaaaaagaa tggagagcga cttcaaaagt cgttctccat tcctatatat cgtatctgtt
     3181 atgtggatgc ttacgctttg ttagctgaac cgaaccagtc gatatgagat ttagcatctt
     3241 caacgattgc tgctttacct ggagctaata atttacgagg gtcgaatcct ttaccttctt
     3301 tatctttacc agcttcgata tatttacgag tagctgcagc aaatgataat tggaattcag
     3361 tgttaacatt tacttttgag atacccattg agatagcttt ttcgatttga tcttgaggaa
     3421 tacctgatcc gccatgtaat actaaaggca tgtctggacc tacagcatca gcaattgctt
     3481 gtaagtggtc aaaagctaat cctttccagt tttctggata ttgaccatgg atgttaccga
     3541 taccgcaagc taagtagtcg atacctgtag caaccatttg tttgcattct tcaatatcag
     3601 ctaattcgcc gttaccgatg attccgtctt cttctccgcc gattgaacca acttcacatt
     3661 caactgagat accttttgca tgtgcttttt caacaacgtc tttagctaat ttcaagtttt
     3721 cttcaaatgg tagatgtgaa ccatcgaaca ttactgaagt atagcctaat tcgatacatt
     3781 ccaaagcagc ttcgtaatca ccatggtcta agtggatagc aactggaaca gtgatgttca
     3841 ttgcttcaac taaatcagtg attaaatctt tacagatttt gtaaccaccc atgtatttag
     3901 ctgcacccat tgatgtttgg ataagtactg gtgcttgttt tgcttcagcc gcttcaagga
     3961 ttgcttgagt ccattccaag ttgtttgtgt tgaatccgcc gacagcgtaa ccgcctttac
     4021 gagcagcttt taaaaattca gctcctgata ctactggcat aataaaattc ctcctaagtt
     4081 ttgtaaaact aaatttgtta agacaattct taaccaacga ccctatttta ccaaacattt
     4141 cggtagtttt ctactaaaat caatgaaaat gaaaattttc aagaagtttt ttgcaaaaaa
     4201 taaaatatcc aatagcgctt taatcgaaaa aagtcttttg ctttctatct tttttcctac
     4261 aaataataac aaatcagaaa aagagaaaag tgacaagaaa attgcttatc cccttctctt
     4321 gctacttttc tctttctatc aagttcaaaa attattttgt ttcgtgatct gctaatgatg
     4381 ctccgacaaa tgctttgatt aatttttgcg ggcgatttgg acgagaaatc aattctgggt
     4441 ggaattggca tcccacaaag aattttttct ctggcagctc tacgatttct actaaacggt
     4501 tatctggtga gacacctgaa aagactagcc cttgttcttc aaacaattga cgatatttgt
     4561 tattgaactc atagcggtga cggtgacgtt cttgcacaac atcggctcca tcatatgctt
     4621 cagccgtctt cgttcctttt ttgattttac atgggtataa tccaaggcgt aaagttccac
     4681 ccaaattctc gatgttttcc tgatcagcca ttaaatcaat aatattgttt gctgtatctg
     4741 gattcgtttc agctgatgac gcatcttcta atccaacgac gttacgtgca aattcgacac
     4801 aagccatctg cataccaaga caaataccta aaaacggaac gtcattttcg cgagcataac
     4861 gaatcgcttc gattttccct tctactccgc gatcaccaaa tccaccagga accaagattc
     4921 catctgcgtc tttcaaacgt tcttctacat tttctctcgt taattcttgt gctttgatcc
     4981 aatcgatctc gatgtctgaa ttataggcga aaccagaatg tttcaatgct tcaactactg
     5041 agatatatgc atctggtaac tcgacatatt taccgacaag cgcaatccgt acttttttct
     5101 tcaagttcaa gacacgttct tctaattctt tccattctgt catgtctgct tctggtgtat
     5161 ctagttttaa atggtcgcaa acaatttgat ccattccttg tgcttgtaaa actaatggga
     5221 tggagtaaag tgtatctaca tctggcgact cgataactgc ttctggttcg acatcacaaa
     5281 attgtgccaa tttgtttttc acgctttgag aaacaggttg ctctgtacgt accaccaaaa
     5341 tattcggttg gatacctaaa ccacgtagct cttttacact gtgctgcgtt ggtttagttt
     5401 tcatttctcc agcagctttc aagtaaggaa tcaatgttgt atggatatac ataacattat
     5461 ctgtaccgac atctgctttc atttgacgaa gtgcttcaag gaatggcaaa gattctatat
     5521 cccctactgt tccccctact tctgtaatga tcacatctga atcagtcatt ttagctgctc
     5581 gcatgatttt ttctttgatc tcatttgtaa tatgaggaat aacttgaaca gttgctccca
     5641 agtattcgcc cttacgttct ttacgcaaaa cttcagaata gatttttcct gtcgtcacgt
     5701 tggagtattt gttcaaattg atgtcaataa aacgttcgta gtggcccaga tccaaatctg
     5761 tttcagcacc atcatctgtt acgaatactt ctccgtgctg atatgggctc atcgtccccg
     5821 gatccacgtt gatgtatgga tcaaattttt ggatagttac ttttaagccg cggtttttca
     5881 ataagcgtcc taaagaagct gcaacaatcc cttttccgat tgatgaaacg acaccgccgg
     5941 tcacaaaaat atattttgtc ataagttcaa aaactccttt ttaatttatg tgcaggaaaa
     6001 gtctcagaca ataaacaaag ctccctattc ttcaataaga atagggagcc gaaatttctg
     6061 taaacttctg accctctaaa agggtgccca aggtgtataa tacaagctga ctaagggaaa
     6121 gtcaagtact caaatgaata ctatccgctt acaatttttt ctttttcgat atcctttgag
     6181 aaattatttc ttaagtcctt tatgagtgca aaaaaaattt aacccacata ataagcttga
     6241 tataaacata gctaacgtta aacctaagca aatcagaaaa tcgttactga aaaattttaa
     6301 gtgaaataat agataaaaaa ataaaacaaa cattatgata gataatatcc cacctaacaa
     6361 agaactactt tttcctactt gcagcaacag tttatatagc ttatcgtaga aacaatcttt
     6421 ttctttcacc cttatttacc tcctttacgt aaaaaacgta gtccacagtt ctcggatttt
     6481 tggcatatct tttctattaa ataatatcaa aaacaaaaca attccactaa ttttttcaaa
     6541 gatgaccttt ttttcaacaa aagtgccttg tagggattca ttgaaggtaa cgaatcacat
     6601 aagtaaaaaa taaggagatg cgacaaaagt cgtgcctcct tatttctaaa taaacggtgt
     6661 tctcctgcca actcctcggt cacaagtcac aatatccatc aaacatgaga agctgttttt
     6721 gaaattattt cttgtgattc ggagttaaat ggtaggctca caacctctgg ataaacggta
     6781 ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt
     6841 cacaaaattt ttcttaatac tcgaaactaa acgtcttttt caaaacctct tggtaaacgg
     6901 tgttcaaaag cttgcatctg cggtaataag ttcagccaat tcaaaaacaa gaaaagctgt
     6961 ttttgcttgg ctattcttat tacttgttgc agggcagctt tttcacaacc tctccactaa
     7021 acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact
     7081 atttttccgt tagtttctct aatactcagg tcaaaacgct gatttcacag cctctaattc
     7141 acggtattcc tgttttaata aagttctaga tattgttctc tttcccactc ggaaacagtt
     7201 tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa aatttgagaa gatatgttgt
     7261 cctaatgcgt caatcattac attatcctta cgcaattctt tgatagcatt atggatagtt
     7321 gaaggtaagt cctggatatg tgcagcttct ctttcttcct catccattac ataaatgtta
     7381 cggtcaacag ctgctggcgg tgtgatctga ttacggatac cgtctagtcc tgcttgcaac
     7441 aatactgcca ttgccaagta tgggtttgca gatggatcaa ctgaccgcaa ttccaaacgt
     7501 gtggataaac cgcgagattc tggtacacgt actaatggcg aacgattacg gccactccat
     7561 gcgacataaa caggcgcttc ataacctgga acgagacgtt tataagaatt gactgttgga
     7621 ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac cacctaagaa atgataagct
     7681 gtttcactta gttccaattc accgtcttta tcatagaaaa catttccttc atcattgaac
     7741 aaagacatat tgcaatgcat accagaacca ttgatcccaa ataacggttt cggcatgaaa
     7801 gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa cgagcttgaa ggtttggata
     7861 ttatcacaag catctaccac atctgcatat ttaaagtcaa tttcatgctg tcctggcgct
     7921 acttcatgat gtgaagcttc cacttcaaag cctagacttt ctaattccaa cacgatgtca
     7981 cggcggcaat tttcacctaa atctgttggt gcaaaatcaa aataaccacc tttatcattc
     8041 aaggtcgttg tgatcccacc gtcttcgtct aatttaaata agaagaattc tggttcaggt
     8101 cctaaattga aagaggtaaa accaaggtcg cgcatatctt tcaatgctcg tttcaaattc
     8161 ccgcgaggat cgccggcaaa tggtgtccca tccggattat agatatcaca aattaaacga
     8221 gcaacttttc catgagcact ttcccacggg aagatcatcc aagtggagag atctggatac
     8281 aaatacatat cgctttcttc aattcgaaca aatccttcaa tcgaagaacc gtcaaacatc
     8341 attttatttt ctaatacttt gtcgatctgg ctcactggaa cttctacatt tttgatcgta
     8401 cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt tttcttcctc aatgattcgt
     8461 ttgatttctt cacctgttat ctgtttcttt accatggtat ccctcttcct tttttaattt
     8521 attcagtcaa agaattgtct ttgcaatcct tgcgactgat aatgacctgt tttgcttaac
     8581 ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa cgtcatgatc tgtcaacggc
     8641 ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt cgtatacacg tttgatccca
     8701 gccatattca atccctctga aagatagtct ttaatctcaa gcaacatatc aatatcattc
     8761 agcgaataca tacgtcggtt tccttcgctt cgttccggat gaactaaatc ttgttcttca
     8821 taatagcgaa tttgccgagc agacaaatca gtcagcttca taactgtacc aattggaaat
     8881 acggacatag atcggcgcaa ttccttttct cccatcagac gtcccctttc gttttataag
     8941 ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg catatgttag gttttctaac
     9001 atgagatctc tattttttat aaaataacaa aaaaataaga acacaaaaaa ggtatctagt
     9061 tgattttcgt accagatacc ttttattttt tattatactg tactatcctt tattagtgtt
     9121 tttatt
//
LOCUS       DACDLI010000087         9105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDLI010000087 DACDLI010000000
VERSION     DACDLI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.087"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(92..622)
                     /locus_tag="IV353_002335"
                     /pseudo
     CDS             complement(92..622)
                     /locus_tag="IV353_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            694..1986
                     /locus_tag="IV353_002336"
     CDS             694..1986
                     /locus_tag="IV353_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9010535.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(2198..3040)
                     /locus_tag="IV353_002337"
     CDS             complement(2198..3040)
                     /locus_tag="IV353_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9010536.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(3077..4543)
                     /locus_tag="IV353_002338"
     CDS             complement(3077..4543)
                     /locus_tag="IV353_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9010537.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(4599..6443)
                     /locus_tag="IV353_002339"
     CDS             complement(4599..6443)
                     /locus_tag="IV353_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9010538.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            7045..7997
                     /locus_tag="IV353_002340"
                     /pseudo
     CDS             7045..7997
                     /locus_tag="IV353_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(8042..8675)
                     /locus_tag="IV353_002341"
                     /pseudo
     CDS             complement(8042..8675)
                     /locus_tag="IV353_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         2802 a         1834 c         1488 g         2981 t
ORIGIN      
        1 taaattatac taccaacaaa tggtagtata ttattaattt atataatatg aaacctaaaa
       61 tatagtagta tacccgtggt gctagttaat tttaataccc taaagaatat ctcaaagtcc
      121 cttcaaaatg ttgctgtgct cggcttaaaa gaatactgta cgataataat atgctttcaa
      181 acttactttc tagcccttga atagaacgtg atttgaaatt ttgaaaacct agtctttcga
      241 gagaagaaaa aacagtttct actgcttttc tttgtctagt aagtaatcga tggtctatct
      301 tccctagttt atccatgttt tttcgtgacg gaactaagac tgtaatgccc ttaagggcaa
      361 gcttttcatg gattttatgg ctgacatagc ctttatctcc aaggatattt ggaaggtttg
      421 cttcttccga taaagtctct aaaactttca catcatgaac actaggatcc gtgattgaat
      481 aagtaactgg aaagcctgtt ttagtgacga taatatgaat tttaagacca taaaaataag
      541 cgtttttcgt tgagttatag cccctacagc tctatacttt ttgatgttgg tggatgacac
      601 cagcatcttt tttattttcc aaaacatata gaaaagacac cttgaaatcc tttagaatta
      661 atttggcgaa ccaacccaaa aggaggaatc aagatgtctt acactcacct tatcaaagaa
      721 acattagata ttctagactt atcagttact tttaacgaaa attgtctcac aaaagaaaaa
      781 tacaaagggc aaatctgtca tatttatcgt ggaaacttga tttatacagc ccaagaatgc
      841 atccactgta aacaccagat tgcttcagat attgtgcgtt ggggtacaac aaccgtccgg
      901 ttgttaatga acgatgtttc tgaatgccgt acctacctcg aattgaaaaa gcaacgcttc
      961 aaatgcaaag cttgtcagcg gacctttgtc gcagatactt ctgttgcgaa aaaacactgt
     1021 ttcatcagtg agaaagtccg ctggtctgtc gtgactcgtc tgaaaaaaaa tacctcaatg
     1081 acagagatcg ccgctcagaa aaatctatcg gtctcttctg tttaccgtat tatgaagcga
     1141 ttttatcgtc cgttgaatcc ttttcggaca ccgttaccta aggtactttg ttttgatgaa
     1201 ttcaaatcag ttcggggtgt ttcaggtgct atgagtttca tcatgatgga tggacaaaca
     1261 cagcgcttgt tggacattgt cgagaaccga caactgcctt ttttaaaacg ttattttagt
     1321 catttttcac gagaaattcg tgaagcggtc gaatggatcg tgattgatat gtacgctcct
     1381 tatgtatcgc tcgttaaaaa gctgtttcca aaagcacagc tgattattga ccggtttcac
     1441 atcgtccaac atattggtcg tacctttcgc aatcatcgaa tcaaagaaac gaaccaacta
     1501 ttaaaaagca aagaacaaaa acactatcaa ttaggtaagc aattaaaaag atactggaaa
     1561 cttcttcaaa aagatgaacg caagcttgat tatactcgac gcctttggcg acctggcttc
     1621 aaagctcatt taacagagac cgatattgtg gatcgcttac tgaaagggag ccctgctttg
     1681 cgtgtaggct atcagttata tcaagacttt ctttacgctg taaaggaacg agactacgta
     1741 tcgtttgaag aactacttac caataatatc atgcttcccg aaggctatca gacgaccttg
     1801 agaacgttcc agaaatttct gccacaaatc aaaaatgctt tacaacaaag ttattcaaat
     1861 ggcccacttg aatgtctgaa caaccatatc aaagtgttaa aacgaaatgc atatggcttt
     1921 cgtagctttt ataatttcaa attgcgcatc atgattcgtc atggcaacgc attgattttc
     1981 aactaaaaaa gatcaagagg aactgatcca cttgatctcc aattattcta agatttctct
     2041 ttaccaacat catttgacaa agagccgaga ttttttatta tcttacgtta atccttttta
     2101 aatgtctctt atagataaca aacagctgga aatcaccttg tttttactat aatccatctc
     2161 taaagatttt aaaagcatca atagtattac tgagcgctca cccattagca tgtttcatca
     2221 accgattgat gtggacacct aggtatacaa cttcatcgtt cggaattgtt ttatgataaa
     2281 ctttttcaat gtagtctttg atcttagctg cgctttccat ggcagacggg tataagatcc
     2341 acatttgatt gtagaattct tcctcaccat catccaacaa agcatcagaa taatatcttt
     2401 ctacaaagaa acgtacatgt gtaatgaagc gggcataatg gatcgaatta gtatcgatat
     2461 ctttctgcaa cgaataccgg accaaattga cgatcccgcc gatcattttt gcatacttga
     2521 atccagcgcc gctatcagga tcttccgatt gtgcattgat caaatggaaa gcaatgtttg
     2581 aggcttcctc tttaggaaga tcaattccat attgctcgtt caaaagggtg agtgcgtact
     2641 tcccaatttg aaattctttg ggatagtaga tttttatttc ccagtacaaa cggtttgcaa
     2701 tatttacccc tttttgtgcg cgttcaactg caaaatgcag gtggtctgtt aaggttaaat
     2761 agactccagg attcagtttt ttatctaatc ctttttctgc ttcaaaaacg atagtacgag
     2821 tgatttcaaa aaattcaata ggtatttctt caactaactc taatagttgt gaagatttct
     2881 tttcttcaaa aggcagaaat attttatcta ctttatctcc tgatatttca tccccagctt
     2941 tctgtccaaa gcctatccct ttacctaatg caatcatttc tttgccattt tcttcaacca
     3001 gtaccacact tgaattcaat acttttttta ttgaaatcac tcattatccc cctatcgctg
     3061 cttaaaaaga tcgagactac tcgaataagg atgcaccgtt tgtttcgatc acttctttgt
     3121 accagttgaa cgaatccttt ttcaagcggt tatatgtccc atttccttca tcgtctgcat
     3181 ccacataaat aaatccatat cgtttagaca tttgagaagt tcctgcacta actaaatcaa
     3241 ttggtcccca gctagtataa gctatcaaat ctacaccttc atcgattgct tctttcattt
     3301 gttggaagtg tgctttaaag tagtcgatgc ggtacggatc atggatttta ccatctacta
     3361 cctgatcgac tgctcccata ccattttcca caatcatcaa cggtacttgg taacgatcat
     3421 aaagttcgat caacgaaatt ttcaaacctt ttggatcgat ttgccagccc caatctgttg
     3481 aaggcaaatg aggattctta acccctaaaa cagtatttcc cggtgtgcgt tctgcgtttg
     3541 gatcagctga ttctgccatg gacatgtaat agctgaaaga aacaaaatct acggtatgtt
     3601 tctttaaaat ttctcgatct tcatcttcca ttttaatctg aattccccga ttttccaaat
     3661 ccttttcgat tagagcagga tattctccct taacttgaac atctgcataa aaattatttt
     3721 ctaagttctt tttcaaggtc agctcaacgt cttctggtgc gcaagttaat ggataagtca
     3781 tcaatttcgt taacatacaa ccaacttggc ttcctggaat tttttcatga gcatcttttg
     3841 tcactaacgc actcgcaaca aattgatgat gcaatgcttg atagatatct tgagcttctt
     3901 gaccttctgc gcttttctct tggataattc cagctgttgt aaatggatga cggtggatgc
     3961 tgtctatctc attaaatgtc agccaatatt ttacgtattt gccgaagcga tcaaaacaaa
     4021 cgttagcgaa gcgaacaaaa tcatcaatta ctcgacgctc cacccagcca ttatatttaa
     4081 ctgctaaagc taatggcatt tcataatgag atagtgtcac aatcggctca attcctaaac
     4141 gttgcatttc tttaaagacg ttttcataaa atgcaacccc tttttcattc ggttccagct
     4201 cttctcctgt agggaacaag cgagtccaag caatagagag gcgcaaggtt ttaaagccca
     4261 tttcggcaaa taaagctaaa tcttctttat agtgatgata aaaatcaacg ccccttcttt
     4321 ttgggtacca tttatcagat ttgtcttcac tagcttcttt tatagactgc agggtgacat
     4381 gcatatgagc agcaaaatct ttgttgtcta aatttggttt ataagtagcc atatccgcta
     4441 ctgaaagacc tttaccgtct acattccaag caccttctac ttgatttgct gctatcgcgc
     4501 cgccccataa aaatcctttt ggaaaacttg ttgtgtttgt cattattcta ttctcctcta
     4561 ttccttcatt caatatattt tttttcacta tcttgtgttt aagctatcgg actgatagct
     4621 aagagatcac tatcattggt taccaatttt tctaggttaa atgaacggat agtgtaatta
     4681 tccttattgg ttaccacaca gataactgtg gggtcgaatc ctgcttcttt gattaaatca
     4741 atatcaaatg caatcagctt ttgtcctttg gctacttttt caccagaacg aacaaatgcc
     4801 tcaaaccctt ttccttctaa ctgaacagta tcaataccaa tatgaaaaag aagttctgca
     4861 ccattagccg tttttaaacc aatagcatgc ttcgtttcaa ataccattgt gatttctcca
     4921 tcttcaggag ctactaatat cccttcttca ggaatgattg ctactccatc acctaccata
     4981 ccactagaaa aaacagtgtc ttttacttct gatagtgaga ttacttttcc attcacggga
     5041 cttgaaagaa cagcatctga aacaacggcc gtttccatct tagtttcaac ttcattgaca
     5101 acttctttat ctttataaag aaccaacgct gcagcaaaag caaccacaaa tgaaatcccc
     5161 gatactgcaa atgcccaaac cagattcatt gggtcagctt tgtcagcgaa catcgtgata
     5221 cttgcaatcc caggacccac taaagcaaag gcttgaagtg caacgattcc tgcaaatgct
     5281 cccccgatca ggctagaaaa cataacggca tataaaacac gtttgtgcag aatcgttacc
     5341 ccgtaaagag ctggttcagt aattccgaat aatgcagata tacccgcaga aacagccgta
     5401 gatttcaatt ttttgtcttt tgttttgata ctgacagcaa agcatgcgcc agcttctgaa
     5461 atattgtgtg ccaatgaagc cgggagataa agaagttctg cacccaattc actcatggat
     5521 gaaacagcgt aagggatcat tgctttatgc atacccgtta caaccattaa tggcagtaca
     5581 gctgctaaga ctccggttgc aacccatcca aattgattgt acaaagacac aatgatagaa
     5641 gagaaagctg ttcctacatt ataacctaat ggtcctagaa gcaacaaagc aataggaact
     5701 gtaatcaata agctcatcat aggtacaaag aaaatacgaa ttggttttgg tgaaactttt
     5761 gtaaaccatt tttcaagttg agcataaaat aaaacagtta agatggctgg gaatacttga
     5821 gatgcatacg ctatattatc caatccaaaa gataagaatg tagcaccttc cgccaacata
     5881 gctgtcattt caggaaggat caatgcaccg acagcagata ctgcaaccaa ttgattgact
     5941 ttcaatttat tagccgtagt cattgccacc agaataggca agaaataaag cggtgccccg
     6001 ccgaccaaat ttaaaatctg ataggtttga gattggtcac taatcagccc taagatagaa
     6061 gctagtagca acaaggactt caatactcct ccacctgcaa tcgcaggaat taaaggttgg
     6121 aaaatagaaa taataaaatc taatagaact gaacctaatt tttgtttttg ttttggtgct
     6181 tcttcagtat ttgaaacatt gtcacctaat aacgctttca cttcatcata cacttcaaca
     6241 acatcgtttc caatgattac ttggcattgc acattttggc gaacgcccat aacgccatcc
     6301 actttttcta actggctcat atttaccttg ctgttgtctt ttaacgtaaa acgtaaacga
     6361 gttgagcaat gctccaaatg ggatatgttt tcttccccac cgatgtactg caatacttct
     6421 tctgctgttt gtttgtaatt catggtgttt ccctcttttc ttttttgagt gatgaacttt
     6481 tgagtcgaag tggccatcct caacacaaaa aacaggcaga aactaaataa attgacaaca
     6541 aggcaaacgt gtattgaaac gcatacattg ttattcaact tatttaggtc ctgcctgcat
     6601 taccagtcac atgcctgttt aagttatatt caatttaaca tattagaatg taaacgtaaa
     6661 taataaaatg attttttaat cattttttca ttttattcgt tcaatatagt tttcgtgttc
     6721 taaaagtttt gtcttttttc taagaatatc ttccaacata tataactctt attctcttct
     6781 atcttctagc agatgttctg tctttttaag acctagtata aaaagaatgg gttcattttt
     6841 tctttcttgc aggatgaggt tttttatata aactttattt gatataaaga aaaggcattc
     6901 ataattgtgc tatactacta aaaaatgata atacagtgaa ttagattagc gctttattaa
     6961 attaagagga cagcatatcg atattgcttc cttttttttg ctatttattt aagggataga
     7021 agtttagaaa ggactgttta taagatgaaa aatgatatag tgacttatgc aaacacaaaa
     7081 tgtgaagaaa ttggcatcag taatttagaa gagtgctttt ttgacacaaa tgacctgtcg
     7141 caatttttaa gaacgggaag aacaaatgcc agtaaacttg ccaatgactt agttgcaaag
     7201 aacgttctat ttaaaatcaa tactcgtcca gtgttatttg ccatcaaaga gcgctttgaa
     7261 gagcactttt tgattccggt aaaagatgta tacgaaagta tatctgaatt aaaaacagac
     7321 ttggagccca attctacaga ttccattcta caaaagatga ttgggtatga tatgagctta
     7381 tccgatgcgg tggaacaaat gaaaactgct gtcttctatc ctggaagagg gcttcctatg
     7441 atgatcactg gagatactgg agttggaaaa agtttttttg ttcatactgc ttatcagttt
     7501 atgaaaaaag tgaacgttat tcatgagaat gctcctttta aagtacttaa ttgtgcgcaa
     7561 taccataata atcctgaatt attgtcgggg atactatttg gctatgttaa aggtgctttt
     7621 accggcgcag atgaagatcg aaatggattg ttgcaagatg cagacggtgg agtactgttt
     7681 ttagacgaag tacacagatt gcctcctgaa gggcaagaga aactatttgt ttatatggag
     7741 atggcggaac ttattctaga gtcggtgaaa ctatccatca cgcctccaat gtacgtttag
     7801 tttttgcgac tacagaaggc aaagaggatt ttctggaaac ctttatgcga agaatcccta
     7861 tacatatcca tatcccaaat attgaggaac gaggtatttt agaaaaaaaa caaattatcg
     7921 aaaatactct ctttagcgaa agcaaaaact ttagcaaggc tattgatgtt tctcctaaag
     7981 tcactagtgt tgcataaata tagtcagaat attctgaatt attattttct tgaaaaaagt
     8041 aaaaaaaatc gtatcccaaa ctttcatcta catttccaga aagatcttca tgttgataat
     8101 caattcctga atcaatgacc gctaccttca catccttaga acccttggtt tcttcccaag
     8161 cggatggcat gctaatctct cttaaattag cttgtttact ccaattagga tcgttatcga
     8221 tttcctaaag aatcagttgt atttggctcc tcaacatcat tcaacggctc tagttcaacc
     8281 acatagtttg gctctgcata attgaccata tcagaagcat taaatgttct tactgcctct
     8341 tccacactaa tttcttcgtt caatttcact aataatactt gatctcgctt attataatcg
     8401 ctttcatttg tcctaagagt caaatctttt tccaccataa cttttacacc caataaattt
     8461 tgcggtttat ttttctctgt tgaaattctt tgtgtagcct ttaaactgac aattagctca
     8521 ttgggtacat actctatttg agagtcatct aattggctca taatcctttc tttttggtta
     8581 gtatctgcag ttacttgttt agtatgagcc atgaacccaa acatacttac cccaacaacc
     8641 aatcctaaat atattttctt tttaaacttt ttcatctaca tctcctccta atagttttat
     8701 tttagagtta gctctattac ttatttacta actcaacaac agattatcat acacttgtta
     8761 atgaaatcac aatatctcat taaaacacac cctaaaatca taaccgttcc tatgctcatt
     8821 ttttggattt tttaatcacg taaccagaaa aataaatccc ctctatttca cgcaaatcaa
     8881 agaagaacta agtaactacc gtttaaaggt agttacttag ttcttctttg attattatct
     8941 aactttatta ttcattcccc accctttgcg cagtgagtct tcggtatctt gttttatctg
     9001 actggtagtt cttattattt ctccacgatc atctaattct ggaacaattg gatatttttc
     9061 tctgaacaag tctagttctt tcaccacgtc attaaacaaa tcttg
//
LOCUS       DACDLI010000088         8859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDLI010000088 DACDLI010000000
VERSION     DACDLI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.088"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..231)
                     /locus_tag="IV353_002342"
     CDS             complement(<1..231)
                     /locus_tag="IV353_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP9010539.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKE"
     gene            complement(322..774)
                     /locus_tag="IV353_002343"
     CDS             complement(322..774)
                     /locus_tag="IV353_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9010540.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(787..1197)
                     /locus_tag="IV353_002344"
     CDS             complement(787..1197)
                     /locus_tag="IV353_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9010541.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1472..1684
                     /locus_tag="IV353_002345"
     CDS             1472..1684
                     /locus_tag="IV353_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9010542.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1687..1899
                     /locus_tag="IV353_002346"
     CDS             1687..1899
                     /locus_tag="IV353_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010543.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(2191..2415)
                     /locus_tag="IV353_002347"
     CDS             complement(2191..2415)
                     /locus_tag="IV353_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9010544.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2493..2681
                     /locus_tag="IV353_002348"
     CDS             2493..2681
                     /locus_tag="IV353_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010545.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            3007..3342
                     /locus_tag="IV353_002349"
     CDS             3007..3342
                     /locus_tag="IV353_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010546.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3575..4516
                     /locus_tag="IV353_002350"
     CDS             3575..4516
                     /locus_tag="IV353_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP9010547.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4518..5408
                     /locus_tag="IV353_002351"
     CDS             4518..5408
                     /locus_tag="IV353_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP9010548.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5451..6251
                     /locus_tag="IV353_002352"
     CDS             5451..6251
                     /locus_tag="IV353_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9010549.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6266..7117
                     /locus_tag="IV353_002353"
     CDS             6266..7117
                     /locus_tag="IV353_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9010550.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7318..7779
                     /locus_tag="IV353_002354"
     CDS             7318..7779
                     /locus_tag="IV353_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9010551.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7805..8257
                     /locus_tag="IV353_002355"
     CDS             7805..8257
                     /locus_tag="IV353_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010552.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8363..8557
                     /locus_tag="IV353_002356"
     CDS             8363..8557
                     /locus_tag="IV353_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010553.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8588..>8859
                     /locus_tag="IV353_002357"
     CDS             8588..>8859
                     /locus_tag="IV353_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010554.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLV"
BASE COUNT         3132 a         1390 c         1825 g         2512 t
ORIGIN      
        1 ttctttctct ttttttaata aaataatatc attttttaac atagtaattt gtttatttaa
       61 atctgatagt gtttcttcct tacttaatat aaatctttct ctatcatgta atttatttag
      121 tttttcatca tttttttgtt ctaattcata agcttctttt tttattgtgt tagctttcga
      181 cagtttagtg attcctataa ttaataaaat aagtccaatc aataataaca ttcaaaactc
      241 ctcatttctt tgatataatt attgtagagt ttactcagaa atgaggaaag agtccatgtg
      301 aaagcatggg cttttttatt tttaatgttg taatactaaa tgttcaagat tatgaggaag
      361 ccctaaatat tcaataattt ggaactgact catatcagat aaatcttgtt catctattgt
      421 tctagttaac atttcaattg caaacatatt agcttcagct tctataccat aaatcataca
      481 actaaggttg tttgctctaa aagctgctgt gcaaatacct ttatggagtc taacgtgggc
      541 tatttcatgc cataatacaa atcttttcat agaaggtgat aggttttgat taattaatat
      601 cacataacat cttttattgt gtatcgtttg tcctaatgtt ttattcccta aatcagtttc
      661 aattaaatct atatcagaat atttgattat tttatatgga ttaatcgcat cagatccaaa
      721 ctctacaaat aatttttcta tttcttctaa tatccagtat ttgtttaaat gcataccaaa
      781 gcccacctat tgacgatatt ttttaggagt gaattttttc tttgctttaa ctttggttaa
      841 ggcaagtgca agttgaatgg catcgtataa tttttcctta tcttcatcgt ctaaaggttc
      901 accataataa tttgtttcag catctgaatt caagccttca agtaattttt cagcttgtaa
      961 agcaatatct tgtttatctt tttctgttaa atcataataa tggcgtttat cagtacgtcc
     1021 taaaagataa tcagttgata cattaaagta atctgcaact ttttgtattt tatctccaga
     1081 tggagtactt ttattccatc tagacatgct tccatttccg aaatttaact ttctttcaag
     1141 ttctgccaac gtcattgaac gttcttttaa taagattttt atacggtcta atagcatagg
     1201 atatgcattc ctttcaaaat accgacaaaa aaagtatttt tctgcaaatt atctatttac
     1261 aaatagagaa tttgcagtta tactagtttt gtaagcaaga tatttacggc atacaaagac
     1321 taaaagcaat aaactaatag tctccccaga catcagtaag ctcgcttttt gtgcgctgta
     1381 ttttgtatgc ttacataata gcatatttgc agtttttgtc aaacttatta gacaaaaata
     1441 ttagaaaata ttcgataagg gggtatgaag catgacttta aaacagcaag tattaattgc
     1501 tttagtaaag aaaggttgga gtcaaagaga gttagctcga cgaatgggta tatcaattac
     1561 ttatttacga gatatcttca tcgaaaaaag aaaacctaag gaacgtttaa aacaaatcga
     1621 agaacttttg gatatcaagt tagaagtaga ttctaatgat cagccatcag aacaggaggc
     1681 gtaaaaatgg tacaacgttt gagcaaagcg gagtttaaag agaaatataa gtattctgaa
     1741 agtacttatc aaagaagaat gaaggaattt aaaaaatcac gttttagtga ggggtataac
     1801 gcagttactt ccaaagaaat aacaattgat gtcgatttat atgaacagtt tagaacatgg
     1861 cgttctaaaa atcgatttag agttagagct gtttgttaat agctaaaaaa tctaaattca
     1921 ccacttctga aaagaggagt tgctgctctc ctttcaagtt tgccaaagtc cacgtttttt
     1981 cattttggca actcctcttc ttggaggtgg tgacggcgag agacgaagga gggatgacat
     2041 gaaggaagaa tcaaaaatag ggattttgaa tgagaacaat tacacaagat taattattga
     2101 aacagaagaa gggaaaaaag tagcagaagt tacattgacc gatgcaactc ctgctgacgg
     2161 ttatcgtatt cgattaactc ctaaatatga ttaacctttt ggtgggtgtg gatcgtttcc
     2221 gtgactatca cggctttgaa tttttccatc tctgccatga ataaatagct cagatccttg
     2281 atttttagaa atctctctag cgatttttac tgcttctgat tttgtattag tatgtgcagt
     2341 cgctttagaa tttccagcac ctttgacatt ccaaccacca ttggaattag gtacaacatg
     2401 ttgatttttt cccatagttt tcacctccat ttctatagaa atattttatc aaatagaaat
     2461 ggagaaattc aataaacgaa aggagaagtg atatggcata taccttgcaa caagaacatc
     2521 aaattctcgg tttgattaaa caacgcagga aacaattaca agatgaccgt gcagcgctta
     2581 gaaaagccga tgagctatca gataggcaag ctgaactaat tgcttctgaa cttgaggatt
     2641 tgagaatgct agaaataaaa aatagggaga ttagattatg aagaagacag acacactttt
     2701 tataggattc attttggggt tattagtgat tgtagcgcac caaagtatta tcgggggaag
     2761 cttgttcgca gcattgatgg ttttaatcaa tctgcttgat tcaaaagaaa ggagcaacta
     2821 tggcacgaga agaagcgcta aaaatcggta aagtgattgc tgataatcgg tggggaaata
     2881 gccgccctat tattttaagc aagcaacata tcgaaaagca aaaagcatgg caacaaataa
     2941 aaaagtgact ctgccagcca gcaaagagtc acatacaaat ttataaaact aggagaatta
     3001 tatcacatga ataagaaaat tgaaaatctt atcgaagaat tgaaacgtga atgccaaaag
     3061 caaggtgttt ctattatttg tacagcccaa aaagaagggg aacttaaaag tctcgtttat
     3121 ggtgagacaa cagaaatttt actttgtctt gctatgcaag aagaacattt agatgaaaat
     3181 ctcccattgt cagcacatat tatgcgtaga attgctgtgg atgcttacga acaagcgaaa
     3241 aatgaagaag aaaatcaacc ttcaaatcat acttttgtta ttaataacaa ggaagattta
     3301 gctgatgtga tgactcgtat tctcaagggg gaatttcaat gatgaatgaa aaaacaattg
     3361 atctactcat tattttattt ttcacgttag tgattgtagc gaaaagttat gtaatctttg
     3421 gcttgttatt cgcaattacc atgcttgtgc tttgcgtgtt agcaagcaga aaagaggtta
     3481 aacatgaacg agttagcaaa tcttgacaac tatttaactg atcctgatta tgctaaacca
     3541 ccatatgaag caccaattga tgaggaggat gaagatgagt aaatctacct tagaaatgag
     3601 ccatcaagaa tggcttgaag accgtaagaa aggcatcgga ggttcggatg ttggaacaat
     3661 tttaggattg aataaatgga aatctcctta tcaactatgg ctagaaaaaa caggacaagt
     3721 agtacttgaa gaatcaggaa gtgagcccgc ttattggggt aatgttttag aagaagtggt
     3781 tgctaaagag tttcaggaac gtacaggcaa aaaagtacgc agaagaaacc aagtatttga
     3841 acatccatta catccatttt taagagcaaa tattgatcgg gaagtagtgg gagaaaatgc
     3901 cattctggaa tgcaaaacag ccaatcaatt tctcggtaaa gaatgggaag gcgaagaggt
     3961 accgcttagc tatctctgtc aagttcaaca ttacatgaac gttctaaaca aagactattg
     4021 ttacatcgct gtcttaatcg gtggtcaaaa atttatctgg aagcggattg aacgagatca
     4081 agagctgatc gatacaatca ctgaacaatt agtagagttt tgggaaacaa acgttcttgg
     4141 aggtatcgag ccaattattg atggtagtca agcgacagct gatttcttga aagagaagta
     4201 tgcagatgtt gaagacgttc aaacgacgtt accaattcat ttcgatgaac tagttgaaca
     4261 gaaaaacgaa ctcaaacgga ctaagaaaga aattgaatca gctatccgtc aagtagacaa
     4321 cgagattatc agcgagctag gtaaacgtga agccagtatc ggtatcactc aaaaaaacat
     4381 catcagttgg aaatttgtcc gtacgagacg tatgaactcg aagaaactag cagagaaata
     4441 tccagatgtc gcaaatgatg aagagattta taacgttact gaatcaagaa ggctaaccga
     4501 aagggagatc aaataatatg gcaacaaatg aatcgttaaa aaatcaattg gcagcaaagc
     4561 cacagaaaca agttgcacca ggtcaattag gacttaaagc tttgatgaat acaccaacaa
     4621 tgagaaagaa gttcgaagag gtgcttcatg acaatgctaa tgcttttatg tcgaatgtta
     4681 tgactcttgt atctaatgac agttatcttg cagatagtga accgatgtct atcatgagtg
     4741 gtgcgttaac tgctgcaaca ttaaatcttg ggctagataa gaatttaggt tatgcatatt
     4801 tagttccatt caatagtaaa aacaagcaaa caggaaaatg ggaaaagaaa gctcaattca
     4861 tgcttggcta taaaggatat atccaattag cccaacgatc aggtaaatac aaagcattaa
     4921 atgtgatcga agtttacgaa ggagaactaa aaagctggaa ccgactgaca gaagagtttg
     4981 agtttgatcc aaatggtaga acgtctgatg aagtcattgg atatgttggc tatttcgagt
     5041 tactgaatgg attcaaaaaa actgtctatt ggaccaaaca agaaatcgaa gctcatcgga
     5101 ttgctaacaa taaagatcgc gataagacaa agttaagtgg tgtgtgggca tctgattaca
     5161 atgcaatggc acgaaaaact gttttgagaa atcttctttc taaatgggga atcttgtcca
     5221 tcgaaatgca agaagccacc acatcggatg agagagtcca aagggttcaa gaagacggca
     5281 gcattattgc tgaaacagaa gttgaagaag atattcctga aagaaaagaa gcagaggtta
     5341 tatctgaaga aaatgaagat gtacaaactg gattatttga tgcatctaat ccgccgttaa
     5401 acaaataatg agggagcttt ctcccttgct tttctagagg agaaatacga atgaacacag
     5461 gatatgtcaa attacatcga aaagtgatga attcattcgt ttggaccaat ccttatatgt
     5521 ataaattatg gaacttgtgt ttgatgaaag cgagtcatga gaatcgcaag atacttttta
     5581 atggaaaaga gatacaagtg aacagcggag aattcgtcac agggcgcgat gctatcacat
     5641 ctgagatgaa cgaaggcgtg aaacgtgaac atcaagtgaa cagcggttct gtatggagat
     5701 ggctaaaaca atttgaaaaa aacggaatgt tgaacatcaa atcaactacg aaatacagcg
     5761 ttatatcaat aaaaaactgg tctttgtacc aaagcagtga acaacaaatg aacatcaaac
     5821 gcacaacaag tgaacaacaa gtgcacacaa acaagaatga aaagaattat aagaatgaaa
     5881 agaataataa aacatcatcg tcgcaaccac gcaaaaagcg tgtttacgac accgactcag
     5941 tttactacat tctcgcggag gagttattca aacagatttg tcagaatcag gaaatcaaaa
     6001 agccgaatct gcaaagttgg gcagatgaca ttcggaaaat gatcgagatc gacaagcgga
     6061 ccgaaaacca ggtacgggga atgattgaat ggagtcagca caatgtgttt tgggcatcga
     6121 atattttatc tgctaaaaaa ttgcgagaaa aatacgacac aatggcagcg caagcaaatc
     6181 gtgattataa aacaaaacaa actaaaacgc ttgaatacga gaaatttagc acagatgagt
     6241 tgcctatttg agaggaggcg taggcatgga aactgttggg gaaatcatgg aaaagctgat
     6301 acagaaagtg cttgtccaac gtggcgaatg tcctgaatgc ggacagcctt tgtatggatg
     6361 gcgtacgaag aaccctgatg gctcagaacg ttgtaagcca acctgtatgc aatgtgggta
     6421 taaagcattg cgagttcagg aagacttaca aacagaacga atttacaacg agagcctgaa
     6481 agcaagagca atcaattttt tcaaaggtgg ttctgttgtg cctaatcaag cgttgtttga
     6541 ttgcacattg cagaattatc aaattgtcga tcaagaaaca agacaagcgg ttgaagtaac
     6601 caaacgcttt gttaattcag tcttgttagg aaatccaagt caccttgttt taactggaaa
     6661 acaaggaaca ggtaaaagtc acctagcgat ggcagcggct tgggaagtct tgaagcgatc
     6721 aaactacgat aaaaaaatct tatttatcgg gttacaggaa atgctggatc aaatcaaatt
     6781 ttcgtacaac aatcctgaac tcagaaaaac gattgaggga tcgttgattg cagatatcaa
     6841 aacagcggat ttagtcatca ttgatgatat cggttcagaa ctaggaaaag atgcatcaga
     6901 tagtcgagcg tttggcataa acacgctaaa ttcgttcttg gatgcacgac agaacctagc
     6961 aacgattatc acaacgaact tgcttggtga agaactgaaa aaagcttacg gtacgagaac
     7021 aatatcaaga atgtttgtca actctgatgg atttacgatg gtattttctc aaacagcaga
     7081 caagcgcata aaaccagtca aaggagcgat cgcatgaatt tgactaaaca agaaaaaaga
     7141 atctgtcgtc agataatgtt gcaaattata ggtgatcgtg acatggacga attatctcaa
     7201 caagagagtc gaatggttcg gaagataagt aagctaatta tttatcggga tacagaaaga
     7261 tttctcactg ttttaggtaa cgtctacgag aatccagagt tattggaggt agagtaattg
     7321 ccaaaaatat tagatgcatg ttgcggtagt agattgttct ggtttgataa gaacaattct
     7381 gacgtaacat ttatggattg tcgtcagcag tatgaagaat tatcaacagg tcatgtgatc
     7441 aatgttgatc cagatgtggt cgcagacttt cgcgatatgc cttttgaaga tgatgtgttc
     7501 gatatggttg tatttgaccc gccacactta agacatgtcg gcgaaaactc atggcttgcc
     7561 aaaaaatatg gaaaattaga cgaactttgg ccagaagaca tcaggcaagg ttttgcagag
     7621 tgtatgcgag ttttacgacc ttcaggaaca ttaattttta agtggaacga ggaacagata
     7681 ccactttctg atgtgttaga ggctattgga gaacagccat tgtttggtaa taaacgaagc
     7741 aaaacacatt ggcttgtgtt tatgaaaaaa ttcagctaac ggaggaaaca aaggaggaaa
     7801 agtaatgaag attgtattaa ataaatgttt tggtggcttt gggttgagcc atgaagcaaa
     7861 aatggaaatt tttaaaagaa aaaatattga agtattccca tacatcaata actttagcta
     7921 tgactcagat gatgaataca cgagatacac aggccaaaag ctaggttcga tggattttat
     7981 ctactatttc aaaaaagacc cgaaaatcga caaagttaca ggaatttata gcgaaattga
     8041 ccgactgtac ggaattgctg atgattccag tttttcaagt gattcaaaca gaggtgataa
     8101 ggacctcgtt gctgttgtcg aaaaactagg aagtgaggcc agtggaccat atgcaagtct
     8161 taaagtcgtt gatatacccg atggggctga gtgggaaata agtgattatg acggcgttga
     8221 aactgcacat tatggttttc aaacaggtag tatttagtcc actaatgaca actaaagagg
     8281 aggcagaagg atgagtgaag atgaatactt tgaacagtag tctattaacg acagagattt
     8341 ttaactggaa aggggtgtag tcatggacgc atcggaagta gtaaataaaa ttaatagtct
     8401 caaagaggtt ttcggagacg tcgaagtagt gataaacgca caaggctatg acaacgaatt
     8461 ttttaagcag attaccgaca ttagcattca gcaaggaatg gaagacgaag acggaaactt
     8521 tatcgatgaa gtagccattt tagtgacttg cgagtaagta attgacagct aaagaggagg
     8581 aagcggaatg aaactaaaag acggatttta ctccagcggc cacggcatcg gcggtttaat
     8641 gctagatatg ccgacaaaga atcctaaaac acgtaagaaa ccaaaattca aagtcggtga
     8701 catggttcgc tgcgaagcag aaggattcat ctatccattt cgtggatatg tagaacacgt
     8761 ctataatcac tcagcaatca ttcgcattga aaacacgatg gaatgtgata agtggttagc
     8821 gaaaagcaaa gagaatttag cagtggtgag attggtgga
//
LOCUS       DACDLI010000089         8603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDLI010000089 DACDLI010000000
VERSION     DACDLI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.089"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..925
                     /locus_tag="IV353_002358"
     CDS             <1..925
                     /locus_tag="IV353_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9010555.1"
                     /translation="NIWTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAI
                     QKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTW
                     NNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDG
                     LINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDK
                     EQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDS
                     MVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            915..1655
                     /locus_tag="IV353_002359"
     CDS             915..1655
                     /locus_tag="IV353_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9010556.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1652..4414
                     /locus_tag="IV353_002360"
     CDS             1652..4414
                     /locus_tag="IV353_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9010557.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            4427..5335
                     /locus_tag="IV353_002361"
     CDS             4427..5335
                     /locus_tag="IV353_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP9010558.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            5335..5955
                     /locus_tag="IV353_002362"
     CDS             5335..5955
                     /locus_tag="IV353_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010559.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            5959..6408
                     /locus_tag="IV353_002363"
     CDS             5959..6408
                     /locus_tag="IV353_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010560.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            6408..6902
                     /locus_tag="IV353_002364"
     CDS             6408..6902
                     /locus_tag="IV353_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010561.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            6916..7347
                     /locus_tag="IV353_002365"
     CDS             6916..7347
                     /locus_tag="IV353_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010562.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            7349..7486
                     /locus_tag="IV353_002366"
     CDS             7349..7486
                     /locus_tag="IV353_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9010563.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            7524..7817
                     /locus_tag="IV353_002367"
     CDS             7524..7817
                     /locus_tag="IV353_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010564.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            7814..8038
                     /locus_tag="IV353_002368"
     CDS             7814..8038
                     /locus_tag="IV353_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9010565.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            8035..>8603
                     /locus_tag="IV353_002369"
     CDS             8035..>8603
                     /locus_tag="IV353_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9010566.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT         3090 a         1191 c         1813 g         2509 t
ORIGIN      
        1 caatatttgg acagggttca agcttacatt gataaatatt tggaatgaag tggtaaatca
       61 agctaaatcg atttggttaa acataaagta tttttttatt aatctttgga ttgatataaa
      121 atattttgca attcagaaat ggattgaatt gaagttttct attattcaaa cttggatcga
      181 tttaaaatat aatgccatta ctacttggaa taatattaaa cagtttttca aagatacttg
      241 gaaaaatatt aaagatacgg catacaatac gtggatttct ataaaaaata ccatgattaa
      301 tacatggaat aacattaagg actctttctg gaatattgtt actggaattg ttaattctgc
      361 tgaaaatgca tggacaaatc taaaaaatgg tgtttcaaat gctattaatc gggtaaaaga
      421 aatctttaat tcgttaaggg aagtcaattt atttgaaatt ggcaagaaca tcattgatgg
      481 acttataaat ggtgtaaaag aaaagtggaa tgctttgaaa gatactatca aaggtattgc
      541 aggtaatatc aaaaattgga ttaaaggagc tttagatatt cattctccat caagatggat
      601 gcgtgatatg gttggtaaaa atattgttca aggtattatc gttggtattg ataaagagca
      661 aagtaagtta gatcaaacca tgacaaattt agtaaaaaca ccgtctgttc aacctgtagt
      721 aaatggaata agtcacctac caattacaca aacaaagcaa aatatatctg ctagtgaaac
      781 aaaagaaatt catttacatt taaatgttta tggagattta cctgattcaa tggttcggaa
      841 gattaccaca aaaattaaaa cagaattgac aagacaaatg aaacgagatg ctggtgcagt
      901 aggaggaaca ttatatgcaa cttaagagag gacaattttt tattaatcaa cattattctt
      961 ctgaatttaa tgtgtatatt caaaacaggc ctgcttctgt ttcagctagt cgagtaattg
     1021 aattgagaga gcgagaagga aatgattcta tcattattga taaaggctat tacaaaaatg
     1081 tgactagaaa gattgagtgt tactataaag caccttcaat tgatatggtg caggagtggg
     1141 aagatcgaat cactgaatgg ttagatatgt cgtcttatag tgattttatt ctttactatg
     1201 atgaacaata tatttatcaa gctgtagtaa tagaaggacc tgaatttaaa ggaacaagaa
     1261 aaacaggaaa tattgtccct tttgaattta cagtgagtat tcgaccattt aaggaaaatt
     1321 acaatggtag atttactatt cgacaaacag aaacttttga aatttataac ccagagaagt
     1381 attcttcaaa accgcttatt aaattgagtg gttctggaga tgcttctttt tatattaata
     1441 aggataaata tgatttgaaa tcgttggaca gagaattatt tatagattct aaattagaag
     1501 aggcttatcg aaaattagat ggtaatttag aacatcagga tcaggtcacc ttatttttag
     1561 attttccatt tttatatcct ggaaaaaatg aaattaaatg gacgaaaaat attcattcat
     1621 ttgaaataat gcctaggtgg tggagaaaag tatgaaacca agaatatata gtcctactga
     1681 aacagatttc tcaacgaatg gcttaggaat tttaaaggat tgtacaagat gtggaatata
     1741 tgaagtagct aatggaaaat atgaattaga gttggattat cctttaggaa ctagatttga
     1801 tgaatatttt gaaaatgact atcaaataaa agcaaagcca aacgatcaag aagagtatca
     1861 tatctttttt attgatgata aagatataga tactttttta aatacagtaa ctatttatgc
     1921 tcagagtcgt acaaatcgac ttggaagacg ggtagtcact cttgtggaag tggactctaa
     1981 aactggtcaa gaagcgatat caattattga aactaaaatg gataaaaaat ctgacatacg
     2041 actttattct gatattacgg ctgtttctag cacaatcttt gaagcgagaa atgttttaaa
     2101 ttgtattgct ggtgaacaag gatcattgct tcaatattgg ggcggagaaa ttaaacgtga
     2161 accatttaag ctttctttgt taaagcgaag aggtcgcaat aatgttggaa cgattcggta
     2221 tggaaaagat atgtctggtt tgaaggtcaa attagattgg acaggtataa aaacaagaat
     2281 tattccatat gctgatcctc aaagtgaagt aggtacgact aatcgaattt atggttcgcc
     2341 agtagatagt gcctatatta ataactatcc tgatgtctat acagaacatg ttcagtttac
     2401 agaagaacaa ggagtaaaag acattaagag cttaaataaa atagctaaaa attactttaa
     2461 aacgatcaat cctggctgtg ataagcctaa agtttctatt acggttgaat ttgataagtt
     2521 gactgatagt gaagaagcga aagaatttgc gaagattaga aattatggtc tgtttgatac
     2581 atttaaaata tatcataaaa aatataatat ttatcttgaa tcaaaggtta gtggactaca
     2641 atacgattct ttatctgaaa aaactttgaa attagaagca ggagacattc aagttgcttt
     2701 ttatcagcaa caagctgtaa ctattcagga taaattaaaa gattatgcaa cgaataatta
     2761 tatgagtgat tttaatgatt atgtttcttc aatgattaca ggtcaaggca gtgcaggagg
     2821 atatgtagtt ttatggccaa aagaaaaacc ttccaacatc tttattatgg ataatccaga
     2881 tttagaaaag gcaagagaag tactaagaat gaataaaaat gggattgctt tttctaagaa
     2941 aggatggcgg gggcctttta attcagcttg gacattagat agtatattta atgccaattt
     3001 tatacaaact ggattaatca aagcagatat ctttcaaaat tcttttaaca agactggaga
     3061 tgtattgaaa ttagttaatg gactactcca aatttggaac aacaaaaaga aaataatgga
     3121 gttaactaaa aaaggaatgg aattttggaa ttctaagggt tcaattggaa cgattggaac
     3181 aactgattct gttggtaatc cttttcctgg ggcttctact ccaaccccta ttgaagataa
     3241 ttctttagtt attcgtacaa atggagacgg caaatatatt ttgatttctc ctaaagtagg
     3301 aaaaggatta gttttattag gaaatggtaa agcaatttat tttggagact tagatgtaca
     3361 aggaaaactt acagttaaag gaaaagaaat aattcctgga caaggtggtg gtccatcggg
     3421 aggtggagaa tctactggtg ggtatcctag tgaattaaaa acagatgcag aaaagagagc
     3481 ttggagaatt tacgatattt tgtgtaataa cggatttacg aaacaatctg catgtggaat
     3541 attggggaac attcaacaag aaacaggagg aacttttgat cctgatactg ttcaaatagg
     3601 tggaccagca tacggattag ttcagtggga tggttcttca tatcctcttg ttggtccagc
     3661 aacatgggat ggaaaagttt atgttcaaaa cttatttaat gctgcaggta ttaaagaacc
     3721 aataacgagc ttagatgcac aagttcgatt gcttatttgg acatttacaa atggacaatg
     3781 gatgggcgta gtacaaccta cgacggttga tggatttaag gcctgtactg atccaagaca
     3841 agcagcatat gcttttgaac gaaactatga acgtccggca gcgacacatc ctgaacgtca
     3901 agattatgca gttaactggt ataacaaatt taaagattta aaaccaggag gagctactgg
     3961 agaagcagga ctaaaacatt tagagtcttt gattggacaa cgaattggca atggtcagtg
     4021 ttatggcttg tctgcagaat attcaggata tctgggtggt tgtggcatgg gtgctggaac
     4081 aaaatatggt ttaactcatg tgataggaaa tacttctgca gcatctgata ttggtattgc
     4141 ctatgattgg tctgctgttg gttggaaagt gattcaaaac cctagatatg atcaattagt
     4201 agttggtgca attattaatt gggcaagagg tggacaagtg ggttcatggt ttgcagatgg
     4261 aacttatgga catactggcg ttatccgagg cttagctaat ggtcgtatgc aaacttatga
     4321 acaaaacaca gagttaggta tgatttgtgg aaagttagat cgccagtatt atagtgcaag
     4381 tgcaatttct tccattgtca taccaccaaa atagggaggt gatttaatgg caaagtggaa
     4441 tgtcatatta agtacaacag aaccctataa ttacgtgggg atgattcaag ttcgacaagg
     4501 caataagaat acagaggtta tggaagcgac tatagttgaa aatggtcttc cctacgattt
     4561 atcagaatgt aaggtatatt ttgaatcagt tgtaggtggg aaatatccag tccaattaga
     4621 aacaaaaatt gtggatgcta aaaaagggaa aattaactat atttttgata aatattccat
     4681 gcagtgttta catcgacaaa cagccaattt cattatattt aaaggagaag acttgattgg
     4741 aacaactcaa gacttctctt attttgtcat taatgctgtt tcaaaaacag aaggagaaat
     4801 gggttcttat tggcaatcaa tcgaagattt aattgcggat atgacggact ttattaatga
     4861 aaataaaggc gattttacgg actggatgaa tgaaaggaaa gaagaatttg aacgttggag
     4921 acaagaacag gaacacagtt tccaagattg gagagaagga caagaatcag actatttggc
     4981 atggtttgaa tcaatcaagg atattttaaa gacgattgat ccaggtggag tcatgttagc
     5041 cgaactaacg gatgcgcgag tagatattca aggagttcgt catgcgtcta tttcagagcg
     5101 tttgctggca gatatggact atttatatca gaaattgcga gcaacgcttt tcacgattga
     5161 atacggggag attgaagtga ctgatatttt gcaggatgat ctcttttcag ataatcatga
     5221 agtcgaaaaa gttggaactg tagcattccc gattgaagaa ggagccttga tcatcgcaac
     5281 cgttgatgat ccaaagcaaa atgtgttcac tcttgagaaa gttggggtga tctaatggct
     5341 aaaactaaac gaatgatgga aacggatgaa aaaacgggtg tacaacgcca attctttcca
     5401 atcacacatg cttccgcagt tcttgggtta gaagaaataa tagcaggaga agcaacagtt
     5461 ttatctgtta atggaaaaat tggagccgtc gtcattacta aagaagattt gggattagag
     5521 aatgttctca cagaattacc ctatgcaagt gaagaagatg acggcattat cacggcagaa
     5581 atgtatcaaa aaattttaaa cagtggagaa ggtgactacg tgttgccagt tgccactatc
     5641 gaccgtttgg gtggcataaa agttggtgaa ttattgacga ttgatgagac agggaaagtc
     5701 tctgcagtca gacaatctga tgtcaatttt tcgctggagt taaaagaaaa actcgattca
     5761 ctgaaaaatt atactgctgg agaaaacatc actattgatg aagatggaac aattagttct
     5821 acaggtggta gtgggactgg tggtgtcaat cagtcatatg tagatcaaaa attccaagaa
     5881 gctgtaaatc aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa
     5941 attggggagg tatagagaat gacagatatc gtaaaaataa aacaaagcaa tgtacaggtt
     6001 tatccacaaa ctcattggaa tgctatagaa ggcaaaccaa caacggtgaa gggagataaa
     6061 ggcgatcctg gccaagcagc aacaattacc ataggaaccg tttctagtgg ttcaaccgct
     6121 tctgttacga atgtaggaac atcatctgca gctagattta attttgtttt accaaaggga
     6181 gataaaggtg atcctggaat aaatgccaca actacagctg tagctacaac tacagcaaat
     6241 gggttgatgt cgtccacaga taaaactaag ttggatggaa ttgcagctgg agcacagaaa
     6301 aatccaggta atgctacaac tacgacagca ggtttaatgt cagcaactga caaagctaaa
     6361 ctcgatggat tagccaatat tacatttgag aaagtgggga cggtttaatg gctgatattg
     6421 tgcaattaaa agaagacgga gttgctaaat acttaaaaac gcatgtagaa gctattgatg
     6481 gtaaagaagc attggtgcag acagatggag accaagccat tgcaggacat aagaattttt
     6541 caggttctgt aacaatcaat aataaacgtg ttttgacgac tgatgataac agatatgaag
     6601 tggtaaatct ggttgtcact aacggtaata cagggacagc aaagctttat cgtgaaggaa
     6661 aaacagtttc tatatatttt gtggctttaa acggaaaaag tagtggcggg aatgattcgg
     6721 ttattttaac tgttccagaa ggctatcgga caccaattag ttttgaacaa ctggttgggt
     6781 caatagaccg ttctactttg aacagtgctc aactatctat tggcgcagac ggagccatta
     6841 aatggagaag aaattcaagt tatggatcag cttattcatt tgttatcact tattcaattt
     6901 aagggaggaa atctaatgaa agtagtttac aaatcaatca agccttacgg attcgagcaa
     6961 atcattttga acaatcaaga aaatatccct gaaaactgta cagagattaa accaccagtt
     7021 cctaactgga gaccaagatt tgattttgat aaaaaacagt gggttgagtt agctactgaa
     7081 gaagagaaaa aagggaatgc tgttgatagc gtagatgaat tggctaatat taaggcatta
     7141 tacgaaacgc tgaaggcaga aaatgatgaa ttgaaacagc taaattctaa agcaatgcta
     7201 aacaatgtag caatcaagca agaaaatgtc ttattgaaag aaaagtcaga aagtttagcg
     7261 cagttgaatt caaaaacgat gcttgcttct gtacaaaata ccaaggaaat cgaagaaatc
     7321 aaaaaacaat tacaaggtgg gaagtaacat gtattcatat gaagatatca aactaatgta
     7381 tgactggggc tgtttcacta acgaacaagt aatggttttc gttccgttgt gcattactga
     7441 agaagaagca gataaaatca ttagcaaaga agagagcgca tcttaattga tgtgcttttt
     7501 attttgattc aaggagttgt cacatgatta atttagggga atggggagcg atagcaggat
     7561 caataaccgc tatcgtttct ttgattttat tagtaataaa accaattact gcatctttct
     7621 cgaagattac tgagactctt tcaaaagtaa gtcacaattt agatttgctg actaaagatt
     7681 tagaatcgag caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa
     7741 gattagatac acatacagaa aaattggtgg aacacacgca acaaattaaa actttattta
     7801 gggagagaag aaaatgaata ataaaacgtt cgaagtacta aaatggttcg cactggtaat
     7861 tattcccgca ctagctactt tcgtggggtt agttggtaaa gcgctcaatt ggcagtacac
     7921 agatatctgt gttgtcatca ttactggttt tggcgcgttt ttagggagtg tgttgggtgt
     7981 atcaaatcga acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa acaaatgaaa
     8041 aagaaaatta ctattactgc gatgagcctg ttaacggctc tttttttatt acccattaat
     8101 acgtttgctt atactattaa tgacgagtat aatttagcgc cgaatcaagg agactccaga
     8161 ttagcaattc ctaacaaaat tattttgcac gaaactggaa tagatgcacc agcaagaaac
     8221 gtagccgcca acatgaaaaa taattataac ggaagcaatt cttatactac agatgttatt
     8281 ggtgacggtg ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg gggagctggt
     8341 aacgctaatc cttatgcgcc tgtacagatt gaattacagc gcacatatga taaagcattg
     8401 tttgaaaaaa actatcgagc ttatattgaa tatacaagag atagtgcaaa aaaatatgga
     8461 attccattga ctcttgatca aggaacttct ttatttacaa aaggaatcat ttctcatttg
     8521 tgggtgacaa attatgtttg ggggaaccac acagatccat atggttactt atcgcaaatg
     8581 ggagttagca aagaaaagct tgc
//
LOCUS       DACDLI010000090         8479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDLI010000090 DACDLI010000000
VERSION     DACDLI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.090"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            100..339
                     /locus_tag="IV353_002370"
     CDS             100..339
                     /locus_tag="IV353_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010567.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(701..961)
                     /locus_tag="IV353_002371"
     CDS             complement(701..961)
                     /locus_tag="IV353_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010568.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1146..1643)
                     /locus_tag="IV353_002372"
     CDS             complement(1146..1643)
                     /locus_tag="IV353_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9010569.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1773..2483)
                     /gene="budA"
                     /locus_tag="IV353_002373"
     CDS             complement(1773..2483)
                     /gene="budA"
                     /locus_tag="IV353_002373"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9010570.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2496..4160)
                     /gene="alsS"
                     /locus_tag="IV353_002374"
     CDS             complement(2496..4160)
                     /gene="alsS"
                     /locus_tag="IV353_002374"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9010571.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4366..5041)
                     /locus_tag="IV353_002375"
                     /pseudo
     CDS             complement(4366..5041)
                     /locus_tag="IV353_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(5051..5920)
                     /locus_tag="IV353_002376"
     CDS             complement(5051..5920)
                     /locus_tag="IV353_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9010572.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            complement(5931..6827)
                     /locus_tag="IV353_002377"
     CDS             complement(5931..6827)
                     /locus_tag="IV353_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9010573.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            complement(6880..8139)
                     /locus_tag="IV353_002378"
     CDS             complement(6880..8139)
                     /locus_tag="IV353_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9010574.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            complement(8168..8422)
                     /locus_tag="IV353_002379"
     CDS             complement(8168..8422)
                     /locus_tag="IV353_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9010575.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
BASE COUNT         2615 a         1785 c         1321 g         2758 t
ORIGIN      
        1 actaatagat actataaaag acttgatagt tttgcattga ttttgcctaa tactgagcat
       61 aggtgtatgc taaacgatac tataaggagt gtgtacataa tgaaaagaat tctgtttatc
      121 agtttatcaa atttattgtt acttactgct tgtgggactt ctgcatcaaa gaacatacct
      181 tcagatacgc aaatttcgaa tagtatcgat tcaacaattt ctacaaatga gaaaaattct
      241 ccggtagaaa cttccgatat aaaagtttct acctctgcaa aaaacagggt aaagaaactg
      301 tcagcacgtc tcctttattt caaacaaact ctgaattagc ttcttcatct tcttcagcaa
      361 ataatgatga tagtttcgcg tttactccta actaaatgca acctttacaa atgctttacg
      421 ataacaagcc tgagctaaat gatcctgata tcagttttgc attttttgaa atgattggat
      481 cggatgatct ttttaaaact actagtttgt ctatccgaaa ataggacagt tctggcagta
      541 taggttttta tcgagtttct ccaccaggcc tcgttacaga gacagattct ctaggcgaac
      601 cttttgaata aatcaaaaat catgaacaat cactgccaac taaataaagt gccttcaaaa
      661 gcttggtctt ctcgcttttg aaggcacttt atttaaatct ttatgaaact aaagtatgtt
      721 catatttgtt gctatagtta tatagatatt ctgttgtttc caactcataa acgtgagtat
      781 attttgccac tacttttccg catttttcta atatttcatt gcgatcgacg tcatgatatt
      841 cgtctacgca gatcacaaca caattttcta atttactgat tacttctcca atcacttttt
      901 gtttcaagcc aataggttgg caaatatatt tttttcctaa aatgatagtt tcgttattca
      961 tatttcatcc atccttaatg ttttttcatt atttaaaatg ataataacat taaaatatga
     1021 ttatgaaaat caataacact tgtgatttta taaaaaaata aaaaaaccga aaaacagaaa
     1081 tgcccactgt ctttccggct ctttttattc ttgatttctc ttcttttttg tttcaataga
     1141 cgattttagt tattaataaa tgaaaaagca tttatattaa aatttgctaa taaaagatct
     1201 acatattttt ctagaaatgc ttgatcgatt tcatcatacc ctttcgttac tccgcaatcg
     1261 atatctaaaa caccgattaa attaccttct tttaccattg gcactacgat ttctgattgt
     1321 gcggctgaat cgcaggaaat gtaatttgca tgggttttga catcatcaac gatcaatgtt
     1381 tcttgttttt cagctgattc tccacaaact ccttttccca tcttgatacg tgtgcaagaa
     1441 actcgccctt ggaacggtcc taaaatcaat tcattaccat cgaataaata atatcctgca
     1501 aagacagtct caggcaaagt ctcattcaac aatgcagatg aatttgctaa atttgctaac
     1561 cagttatctt ctgcttctaa taatccttgt tgctgtaata ataaaatttc gtatgcatct
     1621 tttttctgtt tttctgtcca catgataaaa accgctcctt tttgatctat ggagaagatt
     1681 atagctcagt ttttttgtaa acgctaggtt ttctcctcaa aaaaacgaga tacgtcaggt
     1741 taatcacctt gaacgcatct cgctgtcttt ttctattctg cttgttggat ttctgctgct
     1801 aaatcagcat aatcaatcgt ttcgtgaaca aaagtcggat catcaactgg aaaatgctgt
     1861 acaagggacg agatgcggct aatctcaact tttccttcag ccacttcaaa atccattaca
     1921 tgtccgccaa atgtatgtgt atcatcgata aaatgcaggt gaaatcccgc tgcagcaact
     1981 ccttggaata attcaggcgt aaagaacccg actaatgttc cttggacatt gctttgttgg
     2041 aattctggtt gagactctga aatttttgct agtcttctgt aaggtttttc ttgcttcggc
     2101 atgatacgga tgtgcatatt tttgaataat ccagttatct tcactgccaa aaataggttt
     2161 tccccggctt ctcttactaa tatgtctttt ttcaattgtt ctccatctgc agtttttgag
     2221 acagaaaaac tagtatctgg cgtaaaatct gtaattgctg catagggggt aagttcactt
     2281 ccatctaatt gggcaactga gccatctgat cttccttgat aagctacgcc atctaaaaag
     2341 atgacttcac catcaagccc atgcaatgtt cccaatccca gagttccttt ttctaatata
     2401 tgagcaatcg tttctgttcc atccatcaag ccggccatca aagctcctaa tgtcccgtgc
     2461 tggtataaga ttttttcagt catgtcgaca cttccttaat agaattgatc tggtaagatt
     2521 gtttctccca atttctcatt atcacggtaa tcaatcggaa tatcaataat tacgggtcct
     2581 tctgtcgcaa atccttcttc taaagctttt tctaattcct ctacacttgt agctcggatt
     2641 cctttagcgc caaaagcttc tgcatatttc acaaaatcaa ctggaccaaa gtctacacct
     2701 gacgcccgct gatatttcat tttttcttgg aactctacca tattgtagtg tccatcattc
     2761 caaatcaaat gaacgatatt caatttttta cgtaccgccg tttctaaatc ctgggcagag
     2821 aataaaaatc ccccatcgcc ggaaactgat acgatctgtg tatttggacg caccaatgca
     2881 gcagaaatcg cccatggcaa tgcaacacca agcgtttgca taccattact gaataacaaa
     2941 tgacgaggtt cataacttct aaagtggcgt gccatccaaa tataatgact acctacatct
     3001 acggtaacag tcatatcatc tgttactttt gactgaagcg tgttaataac ttctaaagga
     3061 tgcaagatac ctgcttctcc ttttgattga acaatgtccc gctccgttaa ttttgcttga
     3121 agagatgcta aatattcttt tgcatcttct gacacttgtt gaggctctaa tgaacctgtc
     3181 aataaatcaa gagtggctga gatatcaccg atcaattctc tttcaggctg cataaaagga
     3241 tcgatttcag ctggcgcttc atcgatcaca atgatccgcg catctttttc cgcattccag
     3301 tttcttgctt catattcgat tggatcatag ccaattgcaa taactagatc acttcgtttc
     3361 aaaagcatat ctcctggttg gtttctaaat aaacccacac gaccaaagaa atgcgcttct
     3421 aattcacgtg agatcactcc agctgcttgg aatgtctcta caacaggcaa tgctgtcttt
     3481 tcaactaatt gcctgatggc taacgtttct ttttcacttg atcctcgcat acctactaat
     3541 agtaccggta gttttgcttc tctaatgcgt tcagctaggt aacggatatc atctgcagaa
     3601 gcagaaccta atttaggatc gctcaatggc ttgatgacat taccttggac aggtgcatcc
     3661 actacatctt gcgggatact gataaagctc gcgccttttt ttgaactctt agccatacga
     3721 taagcattgg caatcacttc tgaaattgtt tctggatctt ggatttctgc actgtatttt
     3781 gtgattggct ggaacagtgc tgcattatcc atgctttgat gggtcaactt caatagatcg
     3841 cttcgtttta cttgtccggc aattgctaaa actgggtcac cttctgctgt cgctgttacc
     3901 aaacctgtag ctaagttact agcaccagga ccacttgttg cgataactac tcctggctct
     3961 cctgtaatac gtccgattgc ttgtgccata aatgcagcat tttgttcgtg acgagtcacg
     4021 attaattcag gaccttgatc ctctaattca ttaaatacac catcgatttt tgcacctggt
     4081 ataccgaata cataatcaac atgatgatta atcaaacttt ctactacaga tttcgaacct
     4141 tgtttcattt tttcagccat tgaacatcgt cctttttatg ctttattttt aattattagt
     4201 tagaaataat cacaaataag tgatacagtt tctaaacctg tttcattcaa aagtatagca
     4261 tgttttattt catttgttta gccatatgtt tcaaaaaatg aaaaagaatg acagttttta
     4321 cataaaaaac tgtcattctt aattgaaagt ttcaatttat aaagtctaca gatccatagt
     4381 tctcatcaat ttacgattgt tgaagtaaat caaaccaaat acgatagaag gacctagtag
     4441 aatacaccat tggggcttta cgaaaaagaa gagaaacaaa cttgtcaata gtaaaaaatc
     4501 tagtaaactc acataccatt ttttcaacgt atagtagaac gctatccgta gaacttgatt
     4561 tggtttcgac gctggatttc tcacctgaaa ataccagcag ttgatgctaa aggaaactga
     4621 taaacaagca agtaaaacaa gcaagtaaaa caaacaacgg aatcaaccat tttccataag
     4681 aaaaacgtat aaaaaagaaa caatcaaaaa tcattatcac actaactatc caagcaaata
     4741 gccaataaaa aaatcccttt agccaaaatt ggcgaaacgt ctttatgaac aaacgtactg
     4801 gtgaaaaact tttttctttt gctaattctt ctgtcatacc tatcatcacg atgcaacctg
     4861 gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct atcgacaaaa
     4921 ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta cttgccaatg
     4981 attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc tgctctttca
     5041 taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa tatttttgca
     5101 tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc atgattaatg
     5161 cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt gttttatttt
     5221 ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta acgaatgtga
     5281 tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg cgatagattt
     5341 cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg atactactgt
     5401 aatattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc accaacgacc
     5461 acaacgtatc tgttaaaccc acttttctca tcataatgaa ctgcgggatc atataagact
     5521 gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc cctttaaaat
     5581 ttagttcaga aaacccgtaa acagcaaaac tagaagtaaa caactgaatc aacgtgatca
     5641 caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga gaccaaataa
     5701 ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc gttaacactt
     5761 cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc gtcaataaag
     5821 ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt tttgaacgaa
     5881 tttttgcttt ttttctttca tttcgaacac tctcttccat atcacacctc ctaagtacga
     5941 cgcttattcc aagaaaattg aacaaccgta acgcccaaaa caattacgaa caaaacaagt
     6001 gaaatagctg aagcataacc aaaatcaaat tttttgaatg cttcattgta gatttggtaa
     6061 accagcacat ttgtcgctcg accagggccg ccatccgtca tcacttgcac aaggtcaaac
     6121 accttgaaac aattgatgac aagcatgatc gttacaaaga aggttgttgg tctcaacgaa
     6181 ggtaatgtca cattcaaaaa ttgtttccaa cggtttgctc catctagcga agacgcttca
     6241 tatagttctt tttggatact ttgtagtcct gcaaggtata gaatcatgta ataacccatc
     6301 cctcgccaaa tactgacaat tacaattgcc caaagcgccc aatttgaact tgaagtccaa
     6361 cctggaggat gttcaataaa tcgtcttaaa aagatattaa taggtcccac tgttggatga
     6421 aacaacatat tccaaacaac agcaattgct actaaggaag taacatacgg aaagaaaaat
     6481 atcgtacgaa aaagcttcat ccctctaatt ttctgattta gtagtaaagc tagccctaac
     6541 gaacaaacaa gtgtgccagg cactacagca gcggtatata aaaaagtatt tttcaaagaa
     6601 atccaaaatg tctcatcgtt ggccatttta cgaaagttat ctagtccagc aaattctggc
     6661 gttgaaaaag aatcccattt cataaatgct agtacaagcg aaaacaggat tggtattaat
     6721 gtaaaaacga aaaaaccaat gaaattcggg gcgataaacg aataagcgat ccacgttcgt
     6781 tttctacgca aatgatttgt agctttctta tttatactat tctccatttc gatgacgaaa
     6841 ccaagagtgt tttatctctt ggcttctctc ctttcatgat tattcttttt ccgcagctac
     6901 acgtttctcc atatttaccg ttcctttttc cggcgtttca tctccaacca aaactaagtc
     6961 atattcttct tgtaaaatct tatcgattgc aggcccatac gtatcaatcg gaaattcaag
     7021 tttgatttca tcaggagaaa aagctttttt agaaatttca tccgttggca ttccagcgag
     7081 attaaaataa agctgatcaa tctcatccgt acgatatgaa ggaactactc ctactcctgc
     7141 taatactttt gctccttctt cactggctgc aaattcaata aattcttgcg ccgctttttg
     7201 tttctttgag tttttattga cagcaaatgc tgttggtgag ccaaacgtcg tgaccgattc
     7261 tccttttttc ttctgaggaa tagctgtaat tccccattct acttctgtct tacttgcttc
     7321 tttgtttgcg agtatgccag ccatatacca agttcccatg tacatcattg ctgctttttg
     7381 ttcttcaaat tgagactgat aggttacttt cgttgattta gccgtgccaa aatccatctg
     7441 cgcgccttct ttttgcatcc tcaatgctcg atcataataa tcagtcatat attgataatc
     7501 tggttcaaca agattcgcct cgttttgagc agctgcaata gcttggattg tcgaacgcca
     7561 gatatgttga tatgctccgt aaacttgtcc gtcattttta ctaagttttt ttgctaaatc
     7621 ttcatattcg tcccaagtta gattttttgg atattcgatt cctgcatcat caaacatttt
     7681 cttattgtaa tatagtaccc aaaagtccgt tcgatatggc aatgcatatg tttttccatc
     7741 gatatcatac atttcatatg tttcttcagc agcatcagtg tctaattctc taattcgttc
     7801 tgtttgatcg actaactgat ctcgtaaagc ataatttgaa tacgacaaaa gatttttcat
     7861 cgtcaataca tctgtcgtgt ctccagaaga aagcatcgtg gtgagtttcg tatcataatc
     7921 atctgaagca atatctactg ctttcacttt gattcccgtt ttcttttcga atgcttcaaa
     7981 gagcgctttg aattcaggtg ttgtttcata attccatgtc gaaacggtaa tttcatttgc
     8041 tgattcttca ctactgtctt cttgattacc acaagcacca aatacgagtg acattcctaa
     8101 aactattgat ccaatcatca tttttttcca tcgcttcatt atatgaatcc tcctcttctc
     8161 ttatacatca ccagtaacag taccatgttt tagctagacg agtgattcct tctacataaa
     8221 agtaatcgcc ccagatcaca cattcatcta caccttttaa gctgttttta tcataaacgg
     8281 catggagaag cagaccgttt gatccgttaa ctcgtttcgt tgtatagttt ttctgtaatt
     8341 cttccaagat tttgattgct atctcttcgt aatcgaaatg acgaggatct gaaacaggca
     8401 gttgtttgga aagttctagc aatccacatg ccgctatagc tgctgcagaa ctatcttttt
     8461 cctctttgct atcttcttt
//
LOCUS       DACDLI010000091         8373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDLI010000091 DACDLI010000000
VERSION     DACDLI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.091"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..196)
                     /locus_tag="IV353_002380"
     CDS             complement(<1..196)
                     /locus_tag="IV353_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9010576.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYY"
     gene            293..1189
                     /locus_tag="IV353_002381"
     CDS             293..1189
                     /locus_tag="IV353_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9010577.1"
                     /translation="MDVIGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAA
                     ESYTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAV
                     LLPVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMV
                     GFLMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSG
                     SAILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKS
                     IPTGWIIGAILCLITFLLYGNA"
     gene            1237..3366
                     /locus_tag="IV353_002382"
     CDS             1237..3366
                     /locus_tag="IV353_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9010578.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3341..4030
                     /locus_tag="IV353_002383"
     CDS             3341..4030
                     /locus_tag="IV353_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010579.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4438..5124)
                     /locus_tag="IV353_002384"
     CDS             complement(4438..5124)
                     /locus_tag="IV353_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9010580.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(5260..5454)
                     /locus_tag="IV353_002385"
     CDS             complement(5260..5454)
                     /locus_tag="IV353_002385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010581.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5504..5944)
                     /locus_tag="IV353_002386"
     CDS             complement(5504..5944)
                     /locus_tag="IV353_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9010582.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5948..6793)
                     /locus_tag="IV353_002387"
     CDS             complement(5948..6793)
                     /locus_tag="IV353_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9010583.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6942..8360
                     /gene="pepV"
                     /locus_tag="IV353_002388"
     CDS             6942..8360
                     /gene="pepV"
                     /locus_tag="IV353_002388"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9010584.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2558 a         1571 c         1765 g         2479 t
ORIGIN      
        1 cgtagtaacc atttctttgt gattgccgtt cttctgaacg ttcataggca tttgcctgaa
       61 tatattgatt tcgttgttct tccatcagct gattaaacat gatagtcata agtgtttttg
      121 cggtatcatt cgcaccgctt tcgtttataa ttgcttgaat ttcttcgctt tcaaaagtaa
      181 aatgtacttg agtcattgat atcagtccta ttcgagtatg ttttctgcaa aaaacattgt
      241 actcctaact gagatcaatg gcttttttga tgcttcactt ttacacaatt atatggacgt
      301 aatcggtatt tcagtaccgg ttgcactgtt ctcatttatg ggctcgatta tggctggaat
      361 ctttgctaat atcgtcaatt tcaaacaata tcaaacgatt tatgctggtt tcaatccagc
      421 ggctgaaagc tatacttata atgactattt ccaaattggt atgattggga tgattgtaag
      481 cttagttgtg gtactaacgg tcgctaatat atcaatgaac aaaaagaaac gctatgcaat
      541 ggcagcaaac gtaccagctg aaggtggcga cgcaccaatg atctcatggt tagccgtatt
      601 attaccagtt ttaggggtcg ttcttttaga tcttcaaatc atcttaggtt ttattctagc
      661 aggtatctgg gcattgctgt ttactggaaa attgcgcgga ggttataaag aaatctgtcg
      721 tcagtttgcg aaattattta cagatggcgc agtcgatgtt gcaccaatgg ttggtttctt
      781 gatgacctta gcgatgttca ataattcagc tgcgtatgct tctccttatt tttcagctat
      841 ttttggagat tggataccgc aatcaccatt agtcttggca atcgtatttg ctatattgac
      901 accactagga tttttccgcg gtccaatgaa tctagtcggt tcaggctcag ctattttagc
      961 tgtcgtgtta gctgttaatc caacgatgtc gccagccttt ttgttcccgt tatttgcaat
     1021 tacaacgatt gctcctcagc atttagatat tacacaatct tgggttgctt ggggattagg
     1081 ttatacaaaa gtaacttctc gagaatatat gaagaaatcc atcccaactg gttggatcat
     1141 cggtgcgatc ttatgcctga tcacgttctt attgtacggg aacgcctagt aaacaaaacg
     1201 tcgataaaaa cgattagata aatggaggaa aagaaaatga aacgttttgt gcgaatggga
     1261 attgatgttg gcggtacaca tacgaaagca gtcgcaatcg ataatgcgac gcacgaaatc
     1321 atcggaaaat cttcagtgaa aacaacccac gatgatgtcc gtggagtagc tgcaggtgtc
     1381 gttcagtctt ttcaaaattg tttgcgggaa aataatatca gtccagaaga tgttgtgttt
     1441 gttgcacatt ctaccacaca agcgactaat gcactgattg aaggagacgt agcaaaagtc
     1501 ggcgtaatcg ggatggcaaa aggtggatta gaaggttttt tagcaaaacg acaaactaga
     1561 ttgaacgata tcgatttagg gaataagaaa aaaatagaga ttgtcaatgc attcttacct
     1621 gtcaaaaact taaatgtaga ccgtgtttca gaaacgattt cttctttaga aagagaaaga
     1681 gcggaggtct tggtttcttc tatggcattt ggagtagata atggggaacc agaacgagtt
     1741 gtttatgaag cagcatctgt gaaagcgatc cctacaacga tggcttctga catcacaaaa
     1801 ttatatggat tgacaagaag aactcggaca gcagcaatca acgcatctat tttaccaaaa
     1861 atgctagata cggcaacctc gacagaagac tccgtacgag aagcaggggt caatgtttct
     1921 ttgatgatta tgcgcggaga cggcggagta atggaaatca atgaaatgaa aaaacgacct
     1981 gttttaacca tgctttccgg tcctgctgca tcagtcatgg gctctttgat gtacttgaga
     2041 gcgtctaatg gtgtttattt tgaagtaggc ggaacaacaa ccaatatcgg ggtcattaaa
     2101 aatggacgtc cagcaatcga ttattcaatt gtaggtggcc acccaacata tatctcttca
     2161 ttagatgttc gggttctagg tgtggcaggc ggttcgatgg tacgggctaa tcagtcagga
     2221 atcatcgatg tcggtccacg ttctgcacat attgccggtc tagattatgc agtatttacg
     2281 gagacagaaa aaatcaaagg tcctaaggtt gaatttttct ctcctaaaga aggagaccct
     2341 gctgattacg tcaaggtagt gatggaagac ggagaagaag tgactattac aaatacatgt
     2401 gcagccaatg ttcttggatt agtgcaagaa gaacattttt cttatggaaa tgtcccatct
     2461 gcacgaaaag ccatacaggc attagcagac tattgtcata cgacggttga agacatcgca
     2521 gagcagatca tggaaaaatc ctatgctaag attgaaccgg tgattttaga attggcagac
     2581 aagtatcact tggagaaaga tcagatttct cttgtaggtg tagggggcgg cgctgcctca
     2641 ttgattactt actttagtaa taaaatgggt gtgaaatatt cgattcctga aaacgccgaa
     2701 gtgatttctt ctattggtgt agcacttgca atggtccgag atgttgtaga acgtatcatc
     2761 ccttcgccaa gtaaagaaga tatacgatcg ttgaaaaatg aagcgatgaa taaagcgatt
     2821 gaatcaggcg ctacaccaga atcgatcgaa gtccacgtag aaatcgatcc acatacttca
     2881 aaagtaacgg ctatcgctac tggttctacg gaagtgaaag caaccgatct aaccaaggag
     2941 atcacaacag aagaagcact agagcttgct gcagaggata tgcgattaaa taaaaatgaa
     3001 gtttgcttat tggaaaatac acctttcttt tatgtatgcg gcgaacagaa tcgttcaaaa
     3061 aatgctggaa gtcttcggat catcgatcaa aaaggattta tcaaggtcca acgtggacat
     3121 gcttcatgta tgaaaacaac tgcagccaat tatatgacag ctgtcgaaca actttgggaa
     3181 gatatggcag tttatcaaac tgaattgatt gcccgaccag aattttatct ctgcttagga
     3241 gcaagagtct ctgattttac agcgacggat ctggaacaac tacagctttt gatggatctg
     3301 gaagtatcta ctatggaacc ggaggaagaa gtgatcgtcg ttgccggaaa tatcaaacaa
     3361 acataagtta gaagagttac taaacgaatt acaacagatc cccgaagaaa tttggggatt
     3421 ctaccagttt cggcgagatt tattttggaa aaagattcct ttgtccaagc agaaaattct
     3481 tatacaacaa tcaatcgatt gtggtatcga aacggcatgt agcatcaaaa aaaagtaccc
     3541 ttttgccgat gtaggagaaa tctgtgaaca aatggctgtt ccaatcgttt cctgtgaatc
     3601 agagcaaata aacgaacgaa tcacttttgc aacttatgca gaagatgaag ggatccgatt
     3661 gatgacggag ccgttagaaa aattaaaatg ttcaggacta gcttcgatct ctaaagaaac
     3721 agcgcaagct ttgattattg gacatgaact atttcaccac atcgaagctt ctgttaaggg
     3781 aatttatacg caaagcgaaa aaattgtgct gtggcggttg cctttttata cacatcaatc
     3841 aaacattcgt gcattgagcg agatagcagc gatgagtttt tcaaaagaga tgaatcaaag
     3901 cgatttttcg ccttatgtat tagaggcagt cttactttgg ccatataatg aaattcgtag
     3961 ccaagggatc ttagaagaaa taaaagaaat agaaaaaaga tgtacggagt atgatttaga
     4021 ttacaaataa agagactgtg acaaaagtcg tgtcacagtc ctttttttcc gaataaacgg
     4081 tgttctcctg tcaactcctc ggtcacaagt caaaatattc aacaaacatg agaagctgtt
     4141 ttttgaaatt gttccttgtg actcggagat gaacggcagg ctcacgacct cttggaaaaa
     4201 ggtgttcaaa agcttgcatc tgcggtaata agaacagcca agttaaaaac ataagaagct
     4261 atttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctctcaata
     4321 aacggtgtgc aatcagctga ccctcggtat tagctcaaaa aaaacggcaa aatatgagga
     4381 actattttgc cgtttatttc tctaatactc aggtcaaaac gctgattcca aaacctctta
     4441 tttgatgggt ctgtcctgaa tatcttcaaa ataatcgggg taattatgaa tcacgtattc
     4501 ttgctcctcg ataatcatat gtaaatgcat cgtgcaaaga aatccgactt catcaaagtt
     4561 atgatcaatc attgattgaa gcattgtctg atgttcatct aatactcgac cccagtctaa
     4621 ttctgaaatc gtcaaacgta accatctgaa acggttcaag tgcgtactat aactttctag
     4681 ccaatcccaa atttctcgtt ttccagcaat ttcataacaa gttcggtgaa aatcagtatc
     4741 caaatcaaaa aaagcttttt tattttgagc gatcactgcc tgatcggttt gttctagtat
     4801 tttttctagt ttagcttgat cttgcttggt cattttcgca ctgcattctt gcaggatcgg
     4861 tttttccaac ttttcccgcg tataacgggc taatgaagca gatcggatat cgatttttga
     4921 aacataagtg ccggactgtg gaatggtttt tacaagctct tgattgcgca gttggatcag
     4981 tgcttctcga atcggtgtac gcccgatagc tagttgttct tctaacactt tatcggagat
     5041 tttacttccg ggtgctaact cggaataaat gatttgttta cgtatagatt cataagcttg
     5101 ctgttgcaaa ttattgataa ccatttaacc ttcctttcta tgcagatata atctattata
     5161 acataaatcg cttacaaatc gtgtgggcat aaaaacacag tctgcaaaaa aagccctgat
     5221 aggcatgcct attagagcct cgaaaagaaa aatattgaat caatctggtc gtacctcttg
     5281 gatcggtttg acacgaccgc cgattctttt agcaaaagct tcggattctt ttttgtcttt
     5341 gaaaatgagc agattgtcta agtttttttc ccgagtgcga cagccagttt tatcgatgta
     5401 accgtgcatt actttaacga catacatggg gatcacctgc ttttttcttt tcattatacg
     5461 aactactcga cacaatacaa gaaaaatgct tagacggtct ttattaagaa tttacatcat
     5521 ccaaagaagc gtacgcttgg aataactgtt ctaactccgg attgactttg ctcagcttca
     5581 ctaatcgttg cgaatcagaa agtaaaaaac agtgtttact actgccagta gtttttaatt
     5641 ttttgtcttc agttcccaca atttcgtagc taaaatccaa ccgtgttccc gtatactttt
     5701 ggatagttgg gtggatgctc actttttctc cgtaatggat catttcttta tagctgcaat
     5761 ttacttccag aacggggatg atgatcccag attcttcgat ttttttatag ggaaacccta
     5821 gatgtaccaa taaggctacc cgagcctctt caaaccagcg gatataattg ctgtgatgaa
     5881 taatccccat ttgatctgtt tcataataaa aaggttctcg cacataacct gtaaatttct
     5941 ccatgactca tgcctcgttt tccattgatt ttttacaaaa ataattcatc cacttcggaa
     6001 gtgcttcgct gatttgtgtc ggcaaaacaa tatattgttc ttttgccagc tggtctccaa
     6061 tgaaactatg aagataaaca gctgctgcaa tcgtttggtc attttgagga aactgggcta
     6121 aaaatcctgt gatcatccca gctaaagtgt cacccatgcc gcctgttgcc atagcagcag
     6181 aacctaaagg attggtgtag aagtgctggt gaccataaat ttttgtctca tgacttttca
     6241 aaacgagcac agcaccgatt tcctgttgct tctttttgtt ttctttatcc cgttgttctt
     6301 gtatcggtag agaactcaac cgttgccact ccatttgatg aggtgtaagt acagtttgtt
     6361 cgggataggg tagcaaaaga tgattagcaa caagtaaact taaagcagag ccatcaatca
     6421 ccagccattg ttcttttgat tggttttcta gtacttgttt gaaacgttcc aaactcttag
     6481 aagaagtacc caatccggga ccaatcaaga gtacatctgc ttgctttatt acggaaagaa
     6541 actggtcagt atccgtccaa tctacaatca ttgcttctgg cagatgactg tgaagagcac
     6601 tccgattttt ttcatcagtt actacagtcg tgagacccgc tccactgtgt acggcagcta
     6661 aagcactcat aatgattgct ccgccatatt gttcattgcc gccaatcaaa acgatacgtc
     6721 caaatgttcc tttatgtgaa atctttggac gaggtgtgat gactcttttc actagttgtt
     6781 cgtttatcag catcgaaaaa cctcctttta ctcattatag cgtatatttt ttttagagaa
     6841 aaacagataa gaaaaggaat tgtcagattt tttgaactat ttttttctgt aatttgtggt
     6901 atcatgaaca cgaagactta aataagtgga ggattaactt tatgacaatt gattggcaaa
     6961 aagaagtaga agcacgtaaa gaagacttgc ttgaagactt gaaaaatctt ttacgtgtca
     7021 acagcgagcg cgatgattca aaagtaacac cagatgcacc atttggacct ggacctcgcg
     7081 acgcattgaa acatatgtta gcttatggcg aacgtgatgg atttaaagta aaaaatgtcg
     7141 ataactatgc agggcatatt gatcttggcg aaggagacga aacgctaggg atatttggac
     7201 acatggatgt cgttcctgca ggagatggct gggacacaga tccttatgaa ccagtaatca
     7261 aggatggaaa gatctttgca cgcggatcaa gcgatgacaa aggtccaagt atggcagctt
     7321 actatgcgat gaagatcatt aaagaacttg acttgaaact atcaaaaaaa gttcgcttcg
     7381 tagtcggcag cgatgaagaa agcggctggg gcgatatggc ttattatttc gaacatgaag
     7441 aagaaccaga ttttggtttc tcaccagatg ctgaattccc aatcatcaat ggtgaaaaag
     7501 gaaatgtttc tttagcttta cgcttcaaag gcgacaatgc aggagattat gtcttgaaat
     7561 cttttgtttc cggacttcgc gaaaatatgg tgccaggaac agcaactgca gcacttcaag
     7621 taccaagtgc agatgcagct atcgcgatgg aagaagcatt ttaccaattc atcgaagcaa
     7681 atccagtaag cggaacgatt gaagcggaca atacgtatgt aaaaatcgaa ttagttggta
     7741 aaggcgcaca tggtgcaagc ccacaatctg gtatcaatgc aggttctttc ttggcattgt
     7801 tcttagataa ctatgaattc ctaggatctg ctaaacaatt tatccatgta gcagctgctt
     7861 atgtccatga agatttttat ggagaaaaac taggagtcgc ttatgaagat gaaaaaatgg
     7921 gcaaattgac aatgaatgct ggactatttg cttttgaaga aaatggtaca gaggaagcga
     7981 acttcatcaa tatgaatttc cgttttccaa aaggtgtaac agtagacgga ttgcaaagtg
     8041 acatcgaaca aacagtgggg caagaaggcg caacagttac tcgaggtgct cgcgtaatgg
     8101 aaccacatta cgtaccaatg gatgatccat tggtggctac attattgcaa gtctatgaag
     8161 accacactgg agaaaaaggc tacgaacaaa tcatcggtgg cggaacttac ggacgtttgt
     8221 tgaaacgtgg cgttgcttat ggagcgatgt tcccaggata tactgataca atgcatcaag
     8281 caaacgaatt tatgagtctt gatgacttgt tcagagcaac agcaatctat gcagatgcca
     8341 tctatcgttt ggcgaaataa aaatactaaa aaa
//
LOCUS       DACDLI010000092         8296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDLI010000092 DACDLI010000000
VERSION     DACDLI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.092"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..1517)
                     /locus_tag="IV353_002389"
     CDS             complement(51..1517)
                     /locus_tag="IV353_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9010585.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IV353_002390"
     CDS             1904..2500
                     /locus_tag="IV353_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9010586.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IV353_002391"
     CDS             complement(2558..3040)
                     /locus_tag="IV353_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010587.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IV353_002392"
     CDS             complement(3146..3343)
                     /locus_tag="IV353_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010588.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IV353_002393"
     CDS             3638..4396
                     /locus_tag="IV353_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9010589.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IV353_002394"
     CDS             4456..4884
                     /locus_tag="IV353_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010590.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IV353_002395"
     tRNA            4930..5002
                     /locus_tag="IV353_002395"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IV353_002396"
     CDS             complement(5152..5523)
                     /locus_tag="IV353_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9010591.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IV353_002397"
     CDS             5738..5923
                     /locus_tag="IV353_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010592.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IV353_002398"
     CDS             complement(6006..6194)
                     /locus_tag="IV353_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010593.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IV353_002399"
     CDS             complement(6218..6481)
                     /locus_tag="IV353_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010594.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IV353_002400"
     CDS             6639..6896
                     /locus_tag="IV353_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9010595.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IV353_002401"
     CDS             complement(6967..7158)
                     /locus_tag="IV353_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010596.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IV353_002402"
     CDS             complement(7299..7619)
                     /locus_tag="IV353_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010597.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IV353_002403"
     CDS             complement(7642..8076)
                     /locus_tag="IV353_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9010598.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2633 a         1636 c         1314 g         2713 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacg
//
LOCUS       DACDLI010000093         8229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDLI010000093 DACDLI010000000
VERSION     DACDLI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.093"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="IV353_002404"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="IV353_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9010599.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="IV353_002405"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="IV353_002405"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9010600.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="IV353_002406"
     CDS             2592..3377
                     /locus_tag="IV353_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9010601.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="IV353_002407"
     CDS             3390..4241
                     /locus_tag="IV353_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9010602.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="IV353_002408"
     CDS             complement(4365..4817)
                     /locus_tag="IV353_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9010603.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5282..5650)
                     /locus_tag="IV353_002409"
     CDS             complement(5282..5650)
                     /locus_tag="IV353_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9010604.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5800..6282
                     /locus_tag="IV353_002410"
     CDS             5800..6282
                     /locus_tag="IV353_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9010605.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6319..7329
                     /locus_tag="IV353_002411"
     CDS             6319..7329
                     /locus_tag="IV353_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9010606.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7444..8157)
                     /locus_tag="IV353_002412"
     CDS             complement(7444..8157)
                     /locus_tag="IV353_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010607.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1667 g         2440 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa gaaattcgtt ctctcctaaa gtgatattat
     4981 atttaaatat acctgaaaat aatgggcagt attatgaaag atcactgata ttttctcgta
     5041 ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt tactaagtgg
     5101 aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc cttataaaaa
     5161 tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat aggattgaac
     5221 aaaacctgta taagctgatg aatagtttcc tcgccagtaa aaggtttatt cgtataaagc
     5281 tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa atcaattgtt
     5341 caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta tccgtatata
     5401 cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt tccggtgcga
     5461 tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg tcagtaaaca
     5521 taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt atcttagcaa
     5581 aaaggtcttt gatcttttgt tgatgttctt tttctttgat ctgattgatg atcggctgaa
     5641 atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca gaaaaacaat
     5701 agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata tttatatgat
     5761 aagatgtctc aaataataaa atagaaaaga gacataaaaa tgattgactt ggcaaagaaa
     5821 ttcttacacg aaaatatata ttttgagttg tatcatcatc gagccattta tacaaatgaa
     5881 gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa aagcttattc
     5941 ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa acaaacggat
     6001 ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg
     6061 gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga aaaacagatt
     6121 ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc accaggtaga
     6181 ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca gctacttgag
     6241 atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat
     6301 acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc aacgattggg
     6361 gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga atcagcttta
     6421 aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg gacgcaagat
     6481 aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat gatgcaagat
     6541 gaaaatatcc aaatgatctt tccgccttgg ggaggagaat tattgattga gattctcgat
     6601 tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga tattagtacg
     6661 ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac caaccttatt
     6721 gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca
     6781 agagaaggag aatcgattct acagcaacct tctgaaaagt atcaaaaaga gtggccccat
     6841 gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca atcagtcagc
     6901 ggagatgcag taaggatgaa gggacgatta ttaggtggct gtattgatac gatcagtcat
     6961 ttaatcggta ccccttatgg agatgtccaa tcttttaaaa gaaagtataa tgatggaaag
     7021 ccgatcttgt ggtttctaga aaattgtgag ctatcgtcag ttgatttaag aagatcactt
     7081 gttcagatga aactggcagg atggtttgat ggcagctcgg ggatcatgtt cggtagaagt
     7141 gcagcagagg atttgttaga ttataaaatg aaggatgtat atgaagatct gtcaaaggaa
     7201 ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca actgacattg
     7261 atcaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt tttgcaaacg
     7321 tttatttaaa aaattttttc ctgccttgga gtttatcaaa gaccaacaaa tctaaataga
     7381 aaagaaaatg aaagaagctg acagtatttt gccagcttct ttttgtatga tctattagaa
     7441 attttaatta tccataacta tcacattgat cagctgtttc acatcatttg gattacctgt
     7501 aggaatagct actacagatg cattatcaat aatttcttga gttgtttctc taatgaacgc
     7561 tggataagat gtttgatctg caggcatttg ccaatgtgaa ggaactggat aactagtgat
     7621 tgtcccgtca tggttacttg aatacactaa aactccctgg taactgtatt tcccagtgag
     7681 cataaccaca tctttcggat agactgcact ccctgcatca taaggggcga taggtgttcc
     7741 tgcaggataa tgaataacat gtagttcgaa atcacccgat gtatctctaa aagtattacc
     7801 tctaattgct agccaaacac gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg
     7861 atctattgac tgagaagact gtgaggaagt tgtgtctatt gttgaagaag aaatatttgt
     7921 ctcaactgta ctactagatg tagaagaagt attgggctct gagactttac tgcttgttga
     7981 agatgtattc gtttcagtag aagctgattt ctgagtactt gatttttcag ctgaactggt
     8041 ttgtttgatt gaacttttgt ctgtggtcgc agatggatca ctattttctt tactaggaga
     8101 cgtacatcct acaaggaata aagagaccaa caataaaata aaacctaact ttttcatata
     8161 aaatacctca ttattttttt aataccagta tagcataatc ctgaattttt gaatccgtaa
     8221 aaccttgtc
//
LOCUS       DACDLI010000094         8025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDLI010000094 DACDLI010000000
VERSION     DACDLI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.094"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..191)
                     /locus_tag="IV353_002413"
     CDS             complement(<1..191)
                     /locus_tag="IV353_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9010608.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWK"
     gene            519..746
                     /locus_tag="IV353_002414"
                     /pseudo
     CDS             519..746
                     /locus_tag="IV353_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            872..1843
                     /locus_tag="IV353_002415"
     CDS             872..1843
                     /locus_tag="IV353_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9010609.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1984..3297
                     /locus_tag="IV353_002416"
     CDS             1984..3297
                     /locus_tag="IV353_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9010610.1"
                     /translation="MKRRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIISLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3340..4206
                     /locus_tag="IV353_002417"
     CDS             3340..4206
                     /locus_tag="IV353_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9010611.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4203..5051
                     /locus_tag="IV353_002418"
     CDS             4203..5051
                     /locus_tag="IV353_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9010612.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5065..6537
                     /locus_tag="IV353_002419"
     CDS             5065..6537
                     /locus_tag="IV353_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9010613.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWINDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKGLVYDQHYLRLEINLKQATKLELHYAKGPHHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6554..7426
                     /locus_tag="IV353_002420"
     CDS             6554..7426
                     /locus_tag="IV353_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9010614.1"
                     /translation="MIYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(7660..8025)
                     /locus_tag="IV353_002421"
                     /pseudo
     CDS             complement(7660..8025)
                     /locus_tag="IV353_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2463 a         1406 c         1708 g         2448 t
ORIGIN      
        1 tttttccaaa tttgatagag tagtttgcca tattcttgca cccaacgata aatcgtcgta
       61 tgagaaacgt taatgccacg atcatataag atttcttgaa cttcacgata gctaaggtta
      121 taacgaagat agtatcccac ggctacaata atcacatcct gctgaaattg ctttccttta
      181 aaatgattca tcgtcattcc tcctgctatc tttttctatt attctacctt atttgatagt
      241 agatttaaaa ctttgcaaca gaaccacatt aattttgttg gcaaatatag tttccgctat
      301 gaaaaagaat tatcgaatgg aatactacgt ccgttaaata tagagaatta ttgagaatgt
      361 tcgtcttttg gttcctaccg tgcattttcg cacggatgtt ccagatacta tttttgaatg
      421 tataaaattg aaaacacaag aatcctatta aatcaacgat tcttgtgttt ttaatttttc
      481 actaccgtgc attttcgttt ctatgttcca tgtaccccaa tcttgaaaaa ttaattaaag
      541 acttatataa agccattgac gacttagtcg tggtgctacc ggagtatcaa tgtttaacca
      601 gtatcccagg tattggtcct gtttaggctg ctggaatact cgctgaaatt ggtccaattg
      661 aacgtttttc tgatgaaata aaattgacaa agtatgccgg cctatattgg cgacagagtc
      721 aatccggtaa atctgaatac gaaaattttt ttacgaaaag cttcacgtct aatatctatt
      781 ttaaaataaa aatattcctg ctaatttgga atcgtttcct tcttttggta taataatacc
      841 tgaaattctt ttgagaggag ttgttcgtga agtgaaaact actatgaagg atgttgctgc
      901 gttagcagga gttggtgtgg gtacggtatc tagagtaata aatggagtaa aggtgaaaga
      961 ggttaccaaa gaaaaagtaa tggctgcaat tagtgagctt aattatgagc ctgatgaata
     1021 tgccagaggt atgaaaacga atagcagcaa tacaatagct cttattttac ctacgatttg
     1081 gcatcccttc ttttccgagt ttgcctacta tgtagaggaa gctctcagcc aaaaaaatta
     1141 taagcttttt ttgtgtaatg cgaacggcag tgcggaaaaa gaaaaagaat atatccaaat
     1201 ggtcaagcag agtaaagtag acggaattat aggtattacc tattcagaca ttgataaata
     1261 tgtatcatca aatttaccat ttgtcagtat agaccgccat ttttcggaac aggtttctta
     1321 tgtaacggca gataattatt taggtgggca aattgccgct caagaactac ttactcgtaa
     1381 ctgtaaaaac ttgtgctatg ttgggggtgt ttcaccttac cctaacgaaa caaacaatcg
     1441 taaagctggt ttttacaata tttgtgagga aaataaaata gctgtgaagg tattggatat
     1501 gcctgaacca attagcaatt tggaggaaca attgctcgct tttttttcgg ataatcctga
     1561 aattgatagc atttttacca tcaatgattt catggctttg gatgtgataa aagcccaaaa
     1621 aaaactaggg aaggaagcac ctaaggattt tcaaattatt ggttttgatg gagtcaagct
     1681 atcgttgcaa aaagactatt tactatcaac gattgtacag ccgatagagg aaatggccaa
     1741 ggtttcagta gaaacactca tggcgatgat tttaaatgaa gaaccgccta agcgtgtggt
     1801 agtaccggtt ttctttcacg ctggagaaac gactaaagag taaaaaaccg gtttataacg
     1861 attttttttg agataggttg acagaaagtg gtttcaatga tatcttaaaa ccaattaatt
     1921 ggaaacgtta ccaattaatt ggaaaataaa acggaaacgt ttcaaaaaat aggaggaaca
     1981 tcgatgaaaa ggagaatgtt agcaattgca gcaattttag cattaacagc tggaggactt
     2041 gcaggatgtg gcagtaagga gaaaagttca ggcagcagtg attcaggaga aattaagatt
     2101 tgggttcagt tttctgatga aacgcctgaa ggaaaagctt gggcagagat tgtgaacaat
     2161 tttaatgatg aatacgaggg tgaatataaa gctgttactg agtatattcc tcgtagcgga
     2221 agcggcggtg gatatgagga taaagtaaat gctgcaatta caaccaatac ccttcctgat
     2281 gttattacgt tagatggacc gaatactgca gcttatgctc attcaggagt cattgctcca
     2341 ttagatgatt atttgaaaga tgcggacatg gatgatgtat tagacagcat tattcagcaa
     2401 ggaacctacg atggcaaaat gtatgcgttt ggtttctcag agtccagtgt gggtgtctat
     2461 tacaataagt cgttgttcaa agatgctgga attacagaag atcaattgcc gacgttagaa
     2521 gatccttgga cttggacgga atttcaagca atttgccaaa agctgactga taaatacaaa
     2581 acgcctgcaa ttgatatgca aatggcgtcg ggagacgaaa tgctgaccta cgcatataca
     2641 ccatttgtat ggtcgaatgg cggcgatgtc gttaatgctg atggaaccaa agccgaagac
     2701 tattttaacg ataaaaaatc cgttgaagcg ttggaattta ttcaagaatt ggttgaaaaa
     2761 ggttacacca caatcagtcc tattgaaaaa ggatttgaaa ctggcaaata tgccatgctg
     2821 ctgagcggtt cttggacaat ggcagattta gaggaaaact atcaagagat tgattatggt
     2881 attttgcctt accccgtttc agatgcaact ggggagttag tttcaccaac cggcagctgg
     2941 cagttagcaa tgactgagaa aacagagaaa aaggatgctt ctgcggcctt tattaagttt
     3001 gcaaccagta cagaatctag tcgtattatt agtttaggga acagtgttct acctatcaga
     3061 aattcaacag ttgatttaat taaagatgaa gtgtcggaag gaatgcaggt attaattaag
     3121 caaaaccaaa tttctggaca tgcccgccca gtggttgtag cttatccgca agtatctaga
     3181 gcattccaac aagcagttca agatattagc tacttcgagg aaaacccaga tgtacaaaaa
     3241 gtagcagata gtcgcgctgc tgaaatgcaa caggcaattg atacttcact aaaatagaag
     3301 tttctaaagg gggccaatca agtgttaaaa aaaccgacaa tgaaagacaa tttgataggt
     3361 tacggctttc tagcgccagc tttgctgctg ctgacgattt ttctgatcat acccgtaatc
     3421 atggtaattt attatgcctt tactgattat tatttgttaa cgccagatgc tagacaattt
     3481 atcggcttag aaaattttaa gcgactgttc gctgatccaa tttttgtaaa aagtatttgg
     3541 aacacggcta aatttgttgt ttggattatt ccggtccaat taggggcagc tttaggaatg
     3601 gcacttatta tcaacaaaaa tcgtaaagga aatatctttt ttaaagtagc cttttttgcg
     3661 ccagtcgtga tgtccttagt cgttatttcg attttatggt tgtacttgtt aaatcctaat
     3721 gaaggtctgg ttaatacgct atttgcaaag attggaattg cttcgcagcc ttttttgact
     3781 agtcccaaac aagcaatgta tacgcttgtt ttcgtatcgg catggcaagg tgcaggatat
     3841 cagatgctgc tgtttttagg gggaatgcaa aatattccgc aagacgttta tgaggcagcc
     3901 gaaattgatg gtttttctaa gtttcagcaa tttcgctata tcaccatgcc attattaaag
     3961 cccacggcga tttttatttt acttaccaca ttgatttcgg cctttaaact aatcattcag
     4021 ccaatggtaa tgactcaagg tggaccaatg aattctacta tgacaatggt ttattacatt
     4081 taccaaactg gatttacaga ccgtatggta ggctactcca gctccattgc gttaatcttt
     4141 accaccatga ttggtttaat tacaattgct caaagaaaat tggtaaagga ggatgatcca
     4201 gcatgaccaa gaaatttgga ccgctaatga tcgtagaata ttgtttgctg ttccttttag
     4261 cggcattatt cattttccca atgctgtgga tggtagtttc ctctatgaag cctgaagcag
     4321 atgtctatca aaatttagct tccttcaaag cttttttacc tagtttgaat ccagctaatt
     4381 ggtttaaaac ttatcaagaa gttatttccc ggttcagtgt gggaacttat ttattaaaca
     4441 gtatttttta tggtttaacc tttgcctgtg ggtcaatcat tgtaaactct ttggcgggtt
     4501 ttgcctttgc gaaggttaat ttttcaggca agaaaattat ctttggagct ttgttagctc
     4561 tgctgattgt cccgatggag acggttttaa ttccgcaatt taccatcgtt aattcactag
     4621 gcttagtgaa tactcgcttg gcagtgattt taccagcgat ggccagcgcg tttaatatct
     4681 atttgtttag aaatttcttt atcgcgattc cagaagaaat cattgaatca gcgaaattag
     4741 atggtgcaaa tattgttaaa attttctttc acatcatgct gccaatgtct aaaccagcca
     4801 tagccacagt aggtgtctta tcctttatct ccagctggaa tgattatatt tggccgttaa
     4861 tggtcttaac tgacagcagc aaattttcaa tgcaagtggc aattactacg atcaatacta
     4921 cacagccagt ttatatcaat caagtgatgg ctgttttaac cattgcaacg attccactga
     4981 ttattgttta tatcgttgca caaaaatata ttctgcaggg acttggcggt tctggaacgg
     5041 gtataaaata ggagtgaata gtgaatgttg caaaaacttt taaaggccaa tgagtttatt
     5101 gagaaaaatg ctcaaacagt caatcaagaa taccggaata aatttcattt gatggctccg
     5161 attggctgga tcaatgatcc aaatggtttt gtttattatc aagaggagta tcacttgttt
     5221 tatcaatatt atccttacga tagcatctgg ggaccaatgc attggggcca tgctaaaagc
     5281 aaggatctaa ttcactggga gcatttgcca gtagcactgg caccaggtga aagctatgac
     5341 aaggaaggct gtttttccgg cagtgccata gaaaaggatg gcaagctgta tttgatttat
     5401 acaggacaca atgttgtgga agggcaagtt cgacaagttc agtgtttggc agtttcagaa
     5461 gatggcattc actttgaaaa atatgccgga aatccaatca tcgcagaaga tcacttagct
     5521 tctgttgcaa cgaccgaaga tttccgcgat cccaaagtgt ttcagcgaaa agaagactat
     5581 tttactgtag tcgctactaa aactgcagat gctcgtggac aaattgtgat gttccattcc
     5641 aaagatttac taacttggga gttttattcc gtattgctgg agggaactcc agaacaaggc
     5701 attgtatggg aatgcccaga cttgtttgaa ctagatggca aagaagtctt gattctttct
     5761 cctattcaaa tgactccaca aggcaatgct taccaaaata cgaactcaac tgttgctttc
     5821 attggtactg tcgattggca aacaggcaaa tttctagtag agaattttca tgaaatcgat
     5881 ggcggtttag atttttatgc cccccagacc tgtgaaaacg aagcaggcca aagaataatg
     5941 actgcttgga tgcaaatgtg gcagcgtaaa atacctacga atgatctggg ccatggttgg
     6001 tcaggttcca tgaccttgcc tagagagctg agggtggctg ataatcaatt gttgcaacgg
     6061 ccagtttcat caatttatca agtaattgat ccgcaggaaa ggattgaatg ggcgaagact
     6121 gaagagaatc gattcttact gaagggctta gtatatgacc agcactattt acgcttggaa
     6181 atcaatttaa aacaggcaac gaaactagaa cttcactatg cgaagggacc ccaccataca
     6241 ttactgctta actatgatgt tgaacgggag tgctttactg cttccaggga acacatgccg
     6301 tatgatttat caggagctga aaaggaagtg ttgaaaaagc gcagtgtgac gattcctttg
     6361 gaaaataatt gtctaaccat cgaaattttc cgtgatactt cagctattga gctttttcta
     6421 aatgatcgtc tgactatgag tacaactttt tatgagacag aaaaaggaca ggattttctt
     6481 ttgctggcag acagtgaagt aaccttttca ttggaaagcg gcttaattaa tctatagaag
     6541 ggagagctca gtgatgattt atggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     6601 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     6661 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     6721 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     6781 tacctcgacc ccgaaattgg catggcaaaa ttttgatttc atcggtacca tgaaaaaaga
     6841 atttccgatc ccgatttctt ggacaacaga tgtcaatgcg gctgcctatg gtgagtatgt
     6901 cttcggcagt ggcaaaggat tgagcagtgt cgtttactat acgatcggta cgggtgttgg
     6961 cggcggtgcc ttacaagaag ggcgctttat cgaagggttt agtcatccag aaatggggca
     7021 tatgctcgtc gtccctcatc caaaagatga ctttgctggc agctgtccat ttcatggcaa
     7081 ctgtttagaa gggatggccg ctggacccgc catcgaaaaa cgcttaggga aaaaaggaca
     7141 agaggttgca gaagatgatc cttattggga aatcgaagcc tcctatattg ctcaatgtgc
     7201 ctacaataca accttgatgt tttctccaga tgtgatcatt tttggcggtg gtgtgatgaa
     7261 acaacgccat atgctgcaaa acgtccacga cgcatttgca aaattggtca atgggtatgt
     7321 cgaaacacct gagttaagca aatatatcgt gaccccagca ttagaagaca acgctggaac
     7381 gctcggttgt ttggcattgg ctcgagaagc ggtcttacat tcataaacga aaaaaggctc
     7441 ttcgacattt agtgttggta gagaaagata caactgaaaa tttagtcata accagttagg
     7501 cagagtgctt aactggtttt tgttttactt ggaagatcaa gccttgaatg agataaattc
     7561 ttgctcgaaa gttatggaaa tttcgatagc cgtaggctac ttgtattgtg caatagtttt
     7621 ttacaaaaaa aaggggtgcg gatatttttt tggacctttt taacaaatat atctttgctt
     7681 cttccgatat tgaagagggc ttagccctcc taaagataac ttaattcgtt gattattata
     7741 ccaatgaata taattatcca atttgtttat aaactgattg attgttgtat tcttccagtc
     7801 acgttgataa aacatctcgt tttttaatcg tccaaagaaa ccttcgcacg ctgaattatc
     7861 tggtgaataa ccctttttag acatagagtg aagcaagttg tctttgttcc aatagtccaa
     7921 ctaacaatag caccatcgta acaatcgaca agtggtgata agtagatttt ccctgcggga
     7981 attctaaatt cagtaatatc tgttaaccat ttttcatttg gcttc
//
LOCUS       DACDLI010000095         8001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDLI010000095 DACDLI010000000
VERSION     DACDLI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.095"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..360
                     /locus_tag="IV353_002422"
     CDS             <1..360
                     /locus_tag="IV353_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9010615.1"
                     /translation="KVSNYNKKMAAKYVNEEEFCFSKTKIYKRNPFVSEYVKRLAKGI
                     CQLCQEKGPFIKDGVPYLHCHHIEYLSQGGKDVIENCIALCPNCHARIHELELPDDKE
                     KLLKIVEKRENSFSKIP"
     gene            703..959
                     /locus_tag="IV353_002423"
                     /pseudo
     CDS             703..959
                     /locus_tag="IV353_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            1346..1573
                     /locus_tag="IV353_002424"
     CDS             1346..1573
                     /locus_tag="IV353_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9010616.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1589..2755
                     /locus_tag="IV353_002425"
     CDS             1589..2755
                     /locus_tag="IV353_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9010617.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2990..4033
                     /gene="hrcA"
                     /locus_tag="IV353_002426"
     CDS             2990..4033
                     /gene="hrcA"
                     /locus_tag="IV353_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9010618.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4127..4690
                     /gene="grpE"
                     /locus_tag="IV353_002427"
     CDS             4127..4690
                     /gene="grpE"
                     /locus_tag="IV353_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9010619.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4741..6570
                     /gene="dnaK"
                     /locus_tag="IV353_002428"
     CDS             4741..6570
                     /gene="dnaK"
                     /locus_tag="IV353_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9010620.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6721..7887
                     /gene="dnaJ"
                     /locus_tag="IV353_002429"
     CDS             6721..7887
                     /gene="dnaJ"
                     /locus_tag="IV353_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9010621.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2753 a         1343 c         1746 g         2159 t
ORIGIN      
        1 aaagttagta attataataa aaaaatggct gcaaaatatg ttaacgaaga ggagttttgc
       61 ttctcaaaaa caaaaatata taaacgtaat cccttcgtat ctgaatatgt taaacgactt
      121 gcaaaaggta tttgtcaact ttgccaagaa aaagggccat ttataaaaga cggtgtacct
      181 tatttacatt gtcatcatat agaatatttg tcgcaaggtg gaaaagatgt gattgaaaac
      241 tgtatcgcgt tatgtcccaa ttgtcatgct agaattcatg aacttgagtt acctgacgat
      301 aaagaaaaat tgctgaagat cgttgaaaaa agagaaaata gcttttctaa aataccgtaa
      361 tttattttta taggacaaat aagctaatgt gagctatgcc ttctatataa gactaagttc
      421 tattataagt aacgaagtaa ttctcgttcc ttcctgtagt aatatttttt aaataaccta
      481 taggcttctt caaaagtaca tcctccagta aattctaagt actggaggat tctttttcta
      541 gtgttgcttt actcatgaaa aagtagttgg ctacttcatc aaaatttctg aactttttta
      601 atgtataata aagagctcga tcatttacta caaggatgct agctcctaca ttttcacagt
      661 tttttagcaa gtaaattttt ctaaaatcac atcaggaaat gcatgagatt ctttaagaga
      721 ggcaattata gggctatgat tacggttcgt gtaagtgacg ccaaaaaaga atggctttct
      781 tatatggctg aattttatgg gattacttta tctgaacttc taaaaaacta ttcgatggaa
      841 caactagaag atgaatacaa tcgtcaaacg gctgaaattg cccataaaca ttggataaga
      901 agtgggaaaa caacagtatt catggaagaa tccttaatga atttggtggg ctggaatgac
      961 ctgccgttta aaattcacgc ccgaaattca gaagcagctc tgattggcaa atacgtgtat
     1021 tggtagagat agaagataat caattcgttg ttatcgctat tcaagtaggg caccgaagaa
     1081 atgtctatga ttaagagact gtttattttt acaaaaaaat aataattgga atctagttaa
     1141 aactcttgaa gtgagagatt tgactagatt tttttatgaa taaaataagg acaaaattgt
     1201 ttaggaagaa tcatgagtgt aaaaaaatca tccaagaagt cgattcttgg attttttagt
     1261 ggatttgttc gccaaatacg tttagtatgc gtatatgaga ggttgtaagc aaagcgttgt
     1321 ggtctgagca cttaaggagg aatctgtgaa aatagctttt catatttttg cagattttga
     1381 cttaatgccg aaggtcagct ttgcgattac cgtttatcga gaggctgtaa aagcaccgtg
     1441 tagctccgat gacaataagc aagcattttt agagctaggt ttccttgatt ctaaatatgc
     1501 ggcttatttc aatgagttgg tgcttgaacg cgattttaag aaggtcgaaa acaggcacga
     1561 cttttcgacg taaggagaaa gaatttcaat gatagaaaca ataaatcccg cagaaaacaa
     1621 ggtttctgcg tacattcata tcccattttg cgagcatatc tgctattact gtgactttaa
     1681 taaagttttt ttagaagggc agccagtaga tgagtatgtg gaaatgctgc tgaatgaaat
     1741 gagattaatg ctggaaagtc atcctacagc gaatttggaa acactttatg taggaggcgg
     1801 aacaccaact tctttatctg ctaaacaact tgatcgacta ttatcaggtg ctagagagat
     1861 cctgcctttt caaaatggta atgagtttac tgtagaagcg aatcctggtg atttgacccg
     1921 tgaaaaactt caagtgatga aaaactatgg cgtcaatcgt ttatccatgg gagtgcagac
     1981 atttgacaat cgcttattga aaaaaattgg acgtaaacat actgcagaag atgtgtatca
     2041 aacaatgcaa tttttagaag cagaaaattt taccaatgta agcatcgatt tgatttatgc
     2101 attaccggga caaacactag aaggatatcg ggatactttg gaaaaagctt tggctttgga
     2161 tctgcctcac tattctttgt actcattgat cctcgaaaac aaaacaatgt tcatgaactg
     2221 ggtacgacaa ggaagactcg aactgcctga tcaggaaacg gaaaccagaa tgtttgaaga
     2281 gacgattgaa gcaatggaaa agcatggacg tcaccaatat gaaataagta actttgggct
     2341 ctccggacat gaatctcaac ataacctcat gtactggaac aatgaccact attttgggtt
     2401 tggagctggt gctagcggtt atcttggaga tattcgttac cgcaacaaag gaccaatcca
     2461 acactattta cgcccattac gagcaaatga acgcccggta ttggaagaag aaaaattgac
     2521 acgaaaaaat caaatagaag aagaaatgtt cttaggacta agaaaaaaaa cagggattac
     2581 taaaaaacat ttctatgatc gctatcaaaa aacaattgaa tcggtgtacg gtgatgtgct
     2641 aagggaatta gagcaagaag ggttacttgt taatgaagaa gaccgtgttt atttgacacc
     2701 aagagggact tttttgggga acaatgtgtt tgagcgcttt ttatttgaaa aatagtttga
     2761 agaatatttg ttttttatcg aaacttgaaa agaaaaagtt tagaaagttt gacaatgagg
     2821 tattgaagta ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt
     2881 tagagtgcta aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat
     2941 aagtgaatta gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa
     3001 gcgacaaaac gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt
     3061 tggttccaaa acattaatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga
     3121 tatgaaagca ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat
     3181 tccatctgct ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc
     3241 agaagatgat ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga
     3301 gattattaag caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt
     3361 gggacctgaa ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg
     3421 acagattatt gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct
     3481 tcccgaatat atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct
     3541 agtcggagat cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct
     3601 tcataaatat ttccagacga cagaagggat gtctcagtta tttgacaccg ttcttggtca
     3661 tgtttttgaa gaaaaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg
     3721 tcaagatctt gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac
     3781 acaaatgatc actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa
     3841 tgatttattg cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag
     3901 aggtacaata gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt
     3961 cgatgtttat cgaagagaac tagcagctaa acttgctgat tactaccgct cgcttgactt
     4021 gtcaaactcg taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt
     4081 aagagcaatc aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca
     4141 agaagaacta gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc
     4201 tgcagaagat tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa
     4261 agctgagtta gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat
     4321 ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc
     4381 caaaaaatta cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga
     4441 tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc
     4501 actggaagaa gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct
     4561 tcatcaagca gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga
     4621 agtactacaa gaaggctaca aattacatga tcgcgtgtta agaccaacta tggtcattgt
     4681 tgcacaataa ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt
     4741 atgagtaaaa ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa
     4801 ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta
     4861 tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac
     4921 ccaaatacga tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta
     4981 gaaggtaaat catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga
     5041 ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac
     5101 tttaatgatg cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta
     5161 gaacgtatcg tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat
     5221 cgcgacgaaa aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt
     5281 gaactaggag acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg
     5341 gacgactttg ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc
     5401 attgacttgt ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg
     5461 aaaaaagatt tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga
     5521 gaatctggac cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca
     5581 gctgacttag tagaacgtac aaaagtacca gtacgtcaag cgttaaaaga tgcaggtctg
     5641 tcacaatcag aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt
     5701 gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa
     5761 gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt
     5821 gttttacttg acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact
     5881 aaattgattg accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg
     5941 gcagataatc aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct
     6001 gacaacaaaa ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt
     6061 ccacaaatcg aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa
     6121 gatctaggta cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac
     6181 gaagaaatcg aacgtatggt gaaagatgca gaagcaaacg ctgaagcaga taaagctcgt
     6241 aaagaggaag ttgacctacg taatgatgta gacgcattgc tattctctgt ggacaaaaca
     6301 ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgt
     6361 gacgaattaa aagccgctgt tgaagcaaac aacatcgagg aaatgaaaac taaacgcgat
     6421 gcattgaacg aaatcgttca aaacttgact gttaaactat acgaacaagc tgcacaacaa
     6481 caagctcaag aaaatccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat
     6541 tttgaagaag tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa
     6601 agttgctact ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa
     6661 caaaatatga tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct
     6721 atggcaacaa aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat
     6781 gaaataaaga aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa
     6841 cctgatgcgg aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg
     6901 caaaaacgcg ctgcatacga ccaatacggg catgcaggca cagatccaaa ttacggtgct
     6961 ggtggagctg gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt
     7021 tttgaagata tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg
     7081 cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt
     7141 ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtggaaac
     7201 ggtgcaaaac caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatc
     7261 aatgtagaac gccaaacgcc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt
     7321 catggcacag ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag
     7381 aagaaagcac attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg
     7441 cgtttagctg gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg
     7501 gtttttcgtg tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa
     7561 ttgcctttaa gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat
     7621 ggagatgtga aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt
     7681 ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa
     7741 ctgatcacac cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa
     7801 gctggcggca tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat
     7861 gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa aatataaagt agacttgccc
     7921 aaattttcta tttctagtaa aaacgctaag tcattaataa ttaaaaacca gacgaacaat
     7981 catacgttcg cctggttttt t
//
LOCUS       DACDLI010000096         7664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDLI010000096 DACDLI010000000
VERSION     DACDLI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.096"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..677
                     /locus_tag="IV353_002430"
     CDS             <1..677
                     /locus_tag="IV353_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010622.1"
                     /translation="ILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLLS
                     CIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAV
                     MNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGS
                     DVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEA
                     EVAGVD"
     gene            680..833
                     /locus_tag="IV353_002431"
                     /pseudo
     CDS             680..833
                     /locus_tag="IV353_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            856..2061
                     /locus_tag="IV353_002432"
     CDS             856..2061
                     /locus_tag="IV353_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9010623.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            2845..4755
                     /gene="ltrA"
                     /locus_tag="IV353_002433"
     CDS             2845..4755
                     /gene="ltrA"
                     /locus_tag="IV353_002433"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9010624.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            4859..5083
                     /locus_tag="IV353_002434"
     CDS             4859..5083
                     /locus_tag="IV353_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010625.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            5200..5697
                     /locus_tag="IV353_002435"
     CDS             5200..5697
                     /locus_tag="IV353_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9010626.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            5784..6176
                     /locus_tag="IV353_002436"
     CDS             5784..6176
                     /locus_tag="IV353_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9010627.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            6160..>7664
                     /locus_tag="IV353_002437"
     CDS             6160..>7664
                     /locus_tag="IV353_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP9010628.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT         2598 a         1347 c         1625 g         2094 t
ORIGIN      
        1 ttatactgac actgattgaa gccttgcttc atacagattc aaaactgtat attcttgacc
       61 cgaaaaatgc tgaccttgcg gacttaggtt ctgtgatggc aaatgtctac tatagaaaag
      121 aagacttgct ttcttgcatt gaaacattct atgaagaaat gatgaaacgt agtgaggaaa
      181 tgaagcagat gaagaactat aagactggca aaaattatgc ttacttaggt ctcccggcac
      241 acttcttaat ctttgatgaa tacgtcgctt tcatggaaat gctgggaaca aaagaaaaca
      301 ccgcagttat gaataagctg aaacagattg tcatgttagg tcgtcaagct ggcttctttc
      361 taatactggc ttgtcaacgt ccagacgcaa aatatttagg cgacggaatc cgtgatcagt
      421 ttaatttcag agtggcttta ggtcgtatgt ctgaaatggg ctatggcatg atgtttggca
      481 gtgacgtaca aaaggatttc ttcttaaagc gaatcaaagg tcgtggctat gttgatgtag
      541 gaacaagtgt catatcagag ttttatactc cccttgtacc aaaaggatat gatttcttgg
      601 aggaaattaa aaagttatcc aacagcagac agtccacgca ggcgacgtgc gaagcggaag
      661 tcgcaggtgt ggactgatct tgctggctgg tgtggcaata gccacgccag cacttaaccc
      721 ctcggtatct aacagggggg tacaaatcga caggaaacag tcaaaaaaac attagaaaat
      781 cctttggtta caagggattt acaaaatttc agcgtatgtc aaatgggctt taaaagttga
      841 catacgcctt tttgattgga gggattttta ctgaatgaac aaacttggtt acagcattta
      901 aaagaaaaac gcttggctta tggactatct caaaaccgtt tagctgttgc gactggtatt
      961 acaaggcagt atctaagcga tattgaaaca ggaaaagtca agccatcaga ggatttacag
     1021 cagtcccttt gggaagctct ggaacgcttc aatcccgacg ctccccttga aatgctgttt
     1081 gattatgtaa gaattcgctt tccgacaaca gacgtacagc aggtggtcga aaacatctta
     1141 caactgaaac tgtcctattt tcttcatgag gactatggtt tctattctta ttcagagcat
     1201 tatgctttag gcgacatatt cgtcctttgc tcccatgaac tggacaaagg agttctggtg
     1261 gaattgaaag gtcgtgggtg cagacaattt gaaagctatc ttctggcaca acaaagaagc
     1321 tggtatgagt tctttatgga cgttttggtg gctggcggtg tgatgaaacg ccttgacctt
     1381 gccattaacg ataagacagg gattttaaat atccctgtac tcactgaaaa gtgccaacag
     1441 gaagaatgta tctccgtctt ccgcagtttt aaaagctatc gcagtggcga actggtacgc
     1501 aaagaggaaa aggaatgtat gggaaacacc ctctatatcg gttcattaca aagtgaagtt
     1561 tatttctgta tctatgaaaa ggactacgag cagtacaaga aaaatgatat tcccattgaa
     1621 gacgcagaag taaaaaaccg ttttgagatt cgattgaaaa atgagcgtgc ctattatgca
     1681 gtccgtgatt tactcgtcta tgacaatcca gagcataccg cctttaaaat tatcaatcgg
     1741 tatatccgtt ttgtagataa agacgattcc aaacctcgtt ctgattggaa actgaatgaa
     1801 gaatgggctt ggtttattgg gaacaatcgt gaacgattaa aactaaccac aaaaccagag
     1861 ccttactcct tccaaaggac gctgaactgg ctatctcatc aagttgcccc gaccttaaag
     1921 gttgcgatta aacttgatga aatcaaccag acgcaggttg taaaagacat tctcgaccat
     1981 gcgaaactga cagaccgaca caagcagatt ttgaagcaac agtcagtaaa agaacaggac
     2041 gtgataacaa caaaaaaata actcaaatac aaattcattg aatatagaga ggagaacatt
     2101 tttatgaatt ttggacaaaa cctttgtgcg actcgttcct tagtgaaaag ctaaggcact
     2161 aataaaagaa cgaaaaagtt tggttattag gagaactgat aatctgacag gtggaatgat
     2221 gaagtaacgc tctgaaacgc ctgccctgag cctccgacaa gcattgtatg aggaaactca
     2281 gaatgtttga agctcggtaa agacggctga aagataccgt aatagttgat gaacatatat
     2341 cttctatcga aaactatcca gaggtgggta gcgtatccaa caggtcgggg gtctataaaa
     2401 tatctatggt tagaatgttc tataaggaac tgacgaaaca gcgaatgtac gggtctaaat
     2461 ttgaactttg tagaaatgca aaggtacgag aaatcgtagc tggtgtggca aagtaagaat
     2521 ataggttatg aaatgccata taacgttaca ggcggtatca agccagcagg cttatagcta
     2581 gtgacctaag gatatatatg tatagataag attatcggaa cgaggaaagg tattgggttc
     2641 tcatcaagag aataaactga cgaagaacaa taagcagttg aactaatgct gaaaagcaga
     2701 gggcgaacct aagaaattct tgtaatgaga atggaggaat gccctcaagt cgtgtatata
     2761 ttagaaaagt attatgttgt ttcaataagg attacgagta agataaaaaa gctcactcaa
     2821 tttcaagtaa aggagtgaag ccttatgcct aaaaataaaa atgaaacatt gtgtgtagaa
     2881 gatttaagac acgctgaata ttacgaaatg caaaacacct ttgatgattt atatgccaaa
     2941 agtaagaatg gagatatttt cactcatctt atggatatta tcctatcgag agaaaatata
     3001 ttactggcat atcgaaatat caaagcaaat gcaggcagta aaacagcagg aacagacggt
     3061 acaataatta aagatattgg caaattgcca gcagaaacag ttgtcaaaaa agtaagatac
     3121 attgttgcag gcagtccaca tgggtatcga cctaagccag tgaggcgaaa agaaatacca
     3181 aaaccaaatg gtaaaaccag acctctaggt attccttgta tgtgggacag gcttatacaa
     3241 caatgtataa aacaagtatt agaaccaatc tgcgaagcta aatttagtga aaacagctat
     3301 ggttttagac ctaataggtc agttgaaaat gcaataaaag caacctataa ccgcctacaa
     3361 atcagccagc tacattatgt gattgaattt gatattaagg gtttctttga caacgtgaac
     3421 cattccaagc tgataaaaca gatatgggct atgggaatac gggacaagca cttgatattc
     3481 attttaaaaa ggatattaaa agctccaatc aaaatgacta acggtacaat aacatatcca
     3541 gagaaaggaa ctccgcaagg gggaataata tccccactac tagcaaacat tgttcttaat
     3601 gagttagacc attgggtaga aagccaatgg caggaaaatc ctgttacaaa aaactatgtg
     3661 gttcatatca ataaaagcgg tagcccatgt aaaagcaacg cttacaaaga aatgaagaaa
     3721 acaaagttaa aggaaatgta tatggttcgt tatgcagatg attttagagt tttctgccgc
     3781 tacaaggaaa gtgctgaaaa agccaaaata gcaatcactc aatggattga acaacgatta
     3841 aaattggaag tatcacaaga aaaaacaaga attgtaaatg ttagaaaaag atactccgac
     3901 tttcttggtt tcaagattaa aatgataccg agaagaaaga agttagttgt taaatcacat
     3961 ataagtgata aacaattcaa aaaccaaaaa gacaagctgg tatcacaggc aaagaaaatt
     4021 gcttctccct caaaagggaa aacggaatta gaagaaacaa gactttataa ttcaatggta
     4081 ttaggtatgc aaaattatta tcagattgca acgaatgtaa atctggattg tatgaaactg
     4141 aatagagctg taatggctat cttcactaac agattaggta caaacaaaag gggcagattg
     4201 agaaaaacag gcagacaact tacgacaaag gaggcaacaa gatatggtaa atctaaaatg
     4261 cttcggtatg ttgctggcga gggcgaacct attttccctg ttggatatat ccaacacaga
     4321 aaaccaatgg caaaggtgta tttagcgaat tgctacactc tagagggaag aaaatttatc
     4381 cacaccaatc taagtgttga taaattactg atgtaccagt taatgaaatt atctttggaa
     4441 aatcgcacaa tagaatacgc agacaatcga atttcgctat tttctgctca acatggaaaa
     4501 tgtgctataa ctctggaaga atttcaacaa gctaatgaaa tacattgtca tcatattgta
     4561 ccaactggag ctggtggaaa tgacgattat aaaaatctta ttttggtaaa agaggcggta
     4621 caccgtctaa tacacgctaa aacagaagaa acaatacaaa aatatatagc tctattgaag
     4681 ctaaataaag tccagctcat aagactaaat aaatatagag tattagctgg caatcaagaa
     4741 ttaaacttga tataggtcta ctacataaat acttttcaat ataaacacga tggaacgcgg
     4801 agtgcggtga aagtcgcacg ctccgtgtga atggggggaa aagtttgaga tatttataat
     4861 atcaggaact tacctatcca tatataactg gtttctatca aacgctcaat cactggtgct
     4921 tttagcaatc gttgtgattg gcttgtatct tggcttcaag cgtgagttta gcaaactgat
     4981 tggcttttta attattgcga ttattgcggt tggcttagtc ttcaacgctg ctggagtaaa
     5041 agacatttta ctagagctat tcaatcgcat tattggtgct taaataaaac cgttcttttg
     5101 tggaatataa gtggttttct tatgttccgc aaaggaatgg tacaccaaac gaagtgcggt
     5161 agggattttt gaatctctac aaagaaagga cgtgaatata tggacgatat gcaagtctat
     5221 attgcgaatt taggcaaata caatgagggc gaattggtcg gtgcgtggtt tacctttccc
     5281 attgactttg aggaagtcaa agagaaaatc ggcttgaatg atgaatatga ggaatacgcc
     5341 attcatgatt acgagttacc ctttacggtt gacgaataca cttctattag cgaactcaat
     5401 cgcctatggg aaatggtatc ggagttgcca gaagaactac aatcggagct atctgctctg
     5461 ctcactcatt tttcaagtat tgaagaacta agcgaacatc aagaagatat tatcattcat
     5521 tccgattgtg atgatatgtc tgacgtggca cgctactaca ttgaagaaac gggtgcttta
     5581 ggcgaagtgc cagccagtct tcaaaactat attgattatg aatcctatgg tcgggattta
     5641 gacctttcgg gaacgtttat ctcaaccaat cacgggattt ttgaaatcgt ctattaaatc
     5701 tgtcggtaca ttcttactgg cagattttct tgtttacggg gtagtcaaaa gactatcccc
     5761 atttttatga aaggattgat tacatgaaga aaatacgaag ctataccagt atctggtctg
     5821 ttgagaaagt gttgtactcc atcaatgact ttagacttcc gtttcccata acctttacgc
     5881 aaatgacatg gtttgtcgtg tcactctttg cagttatgat acttggcaac ttgccccctc
     5941 tttctatgat agagggagca tttctcaaat actttgggat tcctgtggct ttcacatggt
     6001 ttatgtctac aaaaaccttt gatggtaaaa agccttatgg atttttgaag tctgtcattg
     6061 cttatgcact gcgacgaaag ctgacctatg caggaaaaaa agtaacgctt ggcagaaacc
     6121 agccacaaga agccattaca gcagttagga gtgaatttta tggcatatcc aattaaatat
     6181 attgaaaaca atctggtctg gaataaagat ggggaatgtt acgcttacta tgagcttgtt
     6241 ccctacaatt actcatttct aagtccggaa cagaaaatac aagtacatga ttctttcaga
     6301 cagcttatcg cacaaaatcg tgatggcaag attcatgctt tacaaatcag tacagaatcc
     6361 agcatacttt ctgcacaaga acgttccaaa aatgaagtca ctggaaagct caaaacggtt
     6421 gcctatgaca aaatcgacca acagacagac gctttaatat ccatgattgg cgaaaatcaa
     6481 gtggactacc gtttctttat cggttttaag ttgcttctca acgatcagga gttttctatg
     6541 aaaagtctta ccgttgaagc aaaaaatgct tttacagatt ttgtctatga tgtgaaccat
     6601 aagctgatgg gcgattttgt tagtatgagt aatgatgaaa tcctgcgttt tcagaagatg
     6661 gaaaagcttt tagaaaataa aatctcccgt cgtttcaaaa tccgaagatt agataaggat
     6721 gacttcggct atctgattga acacctttac ggacagacag gcactgccta tgaagagtat
     6781 gagtatcctc tatcaaagaa aaagctggaa catgaaacac tgattaaaac ctatgacctc
     6841 attaagccta ctcgctgtct ggtggaagaa aaacagcgat atttgaaaat ccagcaggaa
     6901 gacgaaaccg cctatgtagc ttactttacc atcaacagta ttgtcggcga actggacttc
     6961 ccgtcctctg aaatcttcta ctaccagcaa cagcaattta cctttccgat tgatacgtca
     7021 atgaatgtgg aaattgtagc gaatcgtaaa gccctatcta ctgtccgcaa taaaaagaaa
     7081 gaactgaaag acttggataa ccacgcttgg caaagtgata atgaaaccag ctctaatgtg
     7141 gcggaagctc tggaaagtgt gaacgagctg gaaaccaatt tagaccaaag caaggaatct
     7201 atgtataagc tgtcctatgt tgtaagggta tcagcaaatg accttgacga acttaaacgt
     7261 cgttgtaatg aagtgaaaga tttctatgat gatttgagcg tgaaactggt acgacctttt
     7321 ggggatatgc tgggcttaca tgaagaattt ttacctgcca gcaaaagata tatgaatgac
     7381 tatattcaat acgtgacctc tgatttcctc gctggtttag gttttggtgc tactcaaatg
     7441 ctgggtgaaa atgaggggat ttatgttggc tacagcttag atactggacg caatgtctat
     7501 ctgaaacctg ctcttgccag tcaaggggtt aagggttcag taaccaatgc gttagcgtct
     7561 gcctttgtcg gttcgctggg tggtggtaaa tcctttgcga ataaccttat cgtctattat
     7621 gcagtgcttt atggggcaca agcagtgatt gtagacccaa aagc
//
LOCUS       DACDLI010000097         7623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDLI010000097 DACDLI010000000
VERSION     DACDLI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.097"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(33..599)
                     /locus_tag="IV353_002438"
     CDS             complement(33..599)
                     /locus_tag="IV353_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9010629.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(599..1555)
                     /locus_tag="IV353_002439"
     CDS             complement(599..1555)
                     /locus_tag="IV353_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9010630.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1555..2211)
                     /locus_tag="IV353_002440"
     CDS             complement(1555..2211)
                     /locus_tag="IV353_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9010631.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2581..4599
                     /locus_tag="IV353_002441"
     CDS             2581..4599
                     /locus_tag="IV353_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9010632.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5026..7440
                     /locus_tag="IV353_002442"
     CDS             5026..7440
                     /locus_tag="IV353_002442"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9010633.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT         2520 a         1461 c         1554 g         2088 t
ORIGIN      
        1 aaaacatatc atttatgttt tgcgtttttt atttatcttt ttttctcaat acaatataaa
       61 ataggcggct ggttcttttg gttgataaac cgataattca gcacattata tgtttcctgt
      121 ggcagtttgg aacaaaaaga atcaaccata tccagttctt tttctccacc ttcatgacca
      181 tagtagacga caaggatcaa tcgtccgcga ggcacaagtc gtttcaatat ttcttccata
      241 gctgtgcgtg tagtctctgg catagtgatg attgctttat cactttttgg gagataccct
      301 agattgaaaa tccctgcttt gagattttgt tgttcagcta tcacttctcc taacgtttcg
      361 tggccttgta gaaacaatga gacacgttct tctaactgac gctgttctag cctttcacga
      421 gtagcatcga ttgcttgttg ctggatatca aaactataga catgaccagt ttttccgatg
      481 tgttctgcta gaaaaaccgt atcgtggcca ttgcccatcg ttgcatctac gacgtgatcc
      541 cctggttgca agatttcttg cagtaatgta tggctgaaat gcaaagcagt ttgcaacatc
      601 agacgctcac ctttttccgt acatcatatt ttccttggaa gctatctctg cgaagaagtt
      661 cttcatcaat tgcattcaag acttcccatt ttttcaagct ccacattggt ccaatcaagg
      721 aatcccacgg cgcatcccct gtcaaacgat ggatgatgat ctcttttggt atcatttcca
      781 gctggtcgca aatgacattg acatagtcag gacggctcat taattgaagg cgaccttcat
      841 gatagtcccg caacattctc gttttgcgca tcagatgcat caaatggagc ttgattcctt
      901 ggatatctga atctaaaatg gtgcgtctga cattttcttt catcatttct aaactttcgc
      961 ctggtaatcc gttgatcaaa tgcgtacaaa cacggatatt atgtttgcgt aatttagcca
     1021 cgccatctaa ataagtttga taatcatgtg ctcggttgat cagttttgaa gtttcttcaa
     1081 aagttgtttg caatccaagt tctacccata aatacatccg ctcgttcagt tcagcaaggt
     1141 aatcaactac ttcatctggc agacaatccg gtcgtgtacc gactgataat cctacgacac
     1201 ctggtaaatt gactacttgc tcaaatcgtt ctttgattat ttcaactggc gcatgggtgt
     1261 ttgtaaaatt ttggaaataa acgatgtatt gttggacatg aggccatttt tgatgcatca
     1321 tgtcgatttc tttctggaat tggattggaa gagggtcttc aggtgcaacg atcatgtctc
     1381 ccgaaccaga tacactgcaa aaagtacaac cgccatgggc aacagtccca tctcgattcg
     1441 gacagtcaaa tcctccatcg atcggcacct tgaaaatttt cccgccaaac tcatttcgta
     1501 aagcgtaatt ccaagaatga tatcttttat ttccttctga ataaggaaaa gtcattaaaa
     1561 gtccttcttt ctgtaagagt tatctatttg ttttcttttt tctctataca ctggataagt
     1621 caggcacagc caagaaagac tgagtaagta gccgccgatg atatcagtag gaaaatgaac
     1681 gcccagataa atgcggctta tcccgacact tactataata aacatcatca gcaattgaag
     1741 caatgtgcgt aaggttcttt tttgaacaaa agctggaagc agaaatagta aagaaccgta
     1801 aagaatcata ctgcctgctg catgtccact agggaaactg taacttgttt caactactaa
     1861 gtgcgccaat gaaggacgct ctcgcatgaa aaatagtttg atcaccgggt tgatgattcc
     1921 agcaattaca ataaatccag aaacgaacca aaatgtttct gccctttttt tcttatacca
     1981 catccataaa gccgcacaag caaataaaat gatgacggta agaggattac cgaatttagt
     2041 cataaagagt tggtagctgt tcagatcagg ataagaagct ctgacaatgc ctgtaaacca
     2101 ctgatcgaaa ccagaaagcc acgacagatg gaactttacg atccagccca aaatgaaaaa
     2161 caggacaaga aagaagctac cagccacctg aaatgatgtt ttatatttca tacaattcct
     2221 cctttgaagc aatgacttat tatagcattt ttggtccagc gaaaaaaacg gtaaagagat
     2281 aaaaccaatg tatgattcct ctttgaataa ccaatattct tataaaaaaa ataactgtaa
     2341 tcggaaagac attttttggt aatgcaaatg aaatgttagg gaaataaaat cagtgttttt
     2401 cgaaatcctt ttaaacaaga acgtagacgt aggaagaact tgaaaatgaa cagaagttgt
     2461 tcattacggt aaattacaat atgattacaa aaaacaacaa agctcttaaa taacttttaa
     2521 taaatattaa gagaagttat aattattaat aatagataaa ttgaagttgg aggatagaag
     2581 atggaaaaca ttgcaagaaa agaacggaga cgtttagaac agaccaaacg ttttcgaaaa
     2641 gcaaaacgag gggcagcaat cgtgggaact gcgatggtag gctgttctgt cgctgcccca
     2701 ttagtacagc cggttcctgt tcaagcggat gaaacacctg ttcagttcgg cgcaagaatc
     2761 aatacagcgg catttatcac agaaattgct acttatgctc aaccaatcgc tcaagccaac
     2821 gatctatatg catcagtgat gatcgcacaa gcagttgtag aaagcggttg gggcagtagt
     2881 gcgttatctc aagcacctta ttataatctt tttgggataa aaggcagcta tcaaggacaa
     2941 accgtctaca tggatacgct tgaattttta aataatcaat gggtaaataa aaaggagcct
     3001 ttccgacaat atccttcttt tgctgaatct ttcaatgaca atgcctatgt acttagaaat
     3061 acttcatttg gcaacggtta ttactatgcc ggtacttgga agagcaacac gaaatcctat
     3121 acagatgcga cagcttgttt gacagggcgt tatgcgactg atcctggtta cgcaggcaaa
     3181 ttaaataata tcatcgcaac ttacggattg acccaatatg atacaccagc tagcggaaac
     3241 gcaggcggtg gagcgactac aggtggcaat aatactggaa gtgcaggtag tactggcggt
     3301 tcaagcaatt ctggtaatac aaactcaggt tcagcaactt cgaccactta tactgtccaa
     3361 tctggtgatt ctgtttgggg aatctcaaat aaattcggta tcacaatgga tcaattgatc
     3421 caatggaaca acatccaaaa caatttcatt tatccaggac aaaaactgac aatcaaaggt
     3481 agccaggaaa atggctcttc gacgaataat tccggtaaca ataccaactc ttcaggaaat
     3541 gctggtacat caaacggagg acaaacaact ggtgcgaaat acactgttca atctggtgat
     3601 tctgtttgga agatctcaaa tgatcacgga attacgatgg ctcaattgat tgaatggaac
     3661 aatatcaaga acaatttcgt ttatccagga caacagctaa tcgtatcaaa tggcggttct
     3721 tctaatgctg gagcaaatac tggctcaaat actgggaata caagtggctc tagttcgtcc
     3781 agctcaagtt cttctaatac agcttcagga acgaaatata cagttaaagc cggagactct
     3841 gtttggagcg tctcaaataa atacggaatt acgatggctc agctaatcca atggaacaat
     3901 atccaaaata actttatcta cccaggacaa caactgactg tttcaaacag tggatcaaca
     3961 aacagcacgt caggtaatac aggttcgtca accaatacaa ataacagcag ttcagttaat
     4021 actggcagca gttcgtctca aggcacttat accgtgaaag caggtgaatc tgtttggagc
     4081 gtggcaaata aaaacggcat cacgatggat cagttaatcg aatggaataa tatcaaaaac
     4141 aacttcattt atccaggaca gcaattgatc gttaaaggtg gaacttcggc aaatacaaat
     4201 acaggttcaa ctacttctgc agcgaaacca aatacaccga atacgtctgc tacgacaagt
     4261 acatcatcca ctggtaatac gatgtatacc gtgaaagcgg gcgagtcagt ttggagcgta
     4321 gcgaacaaac atcatatcac gatggatcag ttaatcgaat ggaataatat caaaaataat
     4381 ttcatctatc caggacagca agtgattgtg aagaaaggtt catctcaatc aacaaaccaa
     4441 caatcagtta ctggttcgaa aacctatact gtgaaatctg gagaatctgt ttggagtgtt
     4501 gctgacagtc atggaattac aatggctcaa ctaatcgaat ggaacaatat taagaacaac
     4561 tttatttatc caggtcaaac gttgattgta gctaaataaa atagctgttc gaagaaataa
     4621 cgattgaatt ccacaaaaat atttgttact ataaggctac ttaaatatga caacacttga
     4681 aaaggaagta gtaaaaagac tgaagtttca gagagcgcat ggctggtgag aatgtgcacg
     4741 gatgcttttg aactcgcctt tgagttgccc ttgctgaata cccgagtaag caagcggcgg
     4801 tgacggccgt tacaacgttc gaggcctttt cactaaggta aaattaggtg gtaccgcgta
     4861 gattacgtcc tataaaaaga gcaatctttt tataggattt ttttatttct tttgtgaagt
     4921 ttgagaaaac aaagcaaaag gaaataaaaa taaacagatt ctgccggttt tttcgttata
     4981 tttataatag aaacaagtgt tttcaataga acaggaggac agcaaatgag ttacaatcat
     5041 aaagaaattg agaaaaaatg gcaaaaatac tgggcaaaaa cgaatacttt caacacccat
     5101 gatgatcctg aaaagccaaa attttacgca ctggatatgt ttccctatcc atccggtcaa
     5161 gggctacatg tagggcatcc tgaaggttat acggcaacgg atatcctttc acgtgtgaaa
     5221 agaagtcaag gatacaacgt gctgcatcca atggggtggg atgcattcgg tctgcctgct
     5281 gaacaatatg cgctagatac aggaaacgat ccagctgagt ttacccaaaa aaatatcgaa
     5341 acattccgac gccaaatcaa ttctttagga tttagttatg actggaatcg cgaagtgaat
     5401 acaactgatc cggagtatta taaatggaca caatggatct ttacgaagct ttatgaaaaa
     5461 ggtctagctt atgaagcaga agtagcggta aactgggtac ctgaattagg aacagttatt
     5521 tcaaatgaag aagtgatcga tgggaaaagt gaacgtggtg gctatgatgt agtcagaaaa
     5581 ccaatgcgcc aatggatgct gaaaatcact gcttatgctg atcgcttatt ggatgatcta
     5641 gaattagttg attggccaga aagcattaaa gaaatgcaaa gaaattggat cggtcgttca
     5701 gtaggagcaa atattgaatt taaagtggct ggtacggata aatcctatac cgtatttacg
     5761 acacgaccag atactctatt cggtgcaact tactcggttc ttgcacctga actagatctt
     5821 gttagagaaa ttacgacccc tgaacaaaaa gctgctgtag aagcttatat cgaagagaca
     5881 gctaaaaaat cagatctaaa gagaacggat cttgcaaaag aaaaaacagg agtatttact
     5941 ggtgcttatg caatcaatcc agttaatggc aaggaaatcc caatctggat cgccgattat
     6001 gtattatctt catacggcac aggagccatc atggctgttc cagcacatga tgaacgtgac
     6061 tttgaattcg ctcaaacttt cggactagaa attcttccag ttatcgaagg tggagatgtt
     6121 gaaaaagctg cttacacaga agatggtacg cacatcaact ctgaatttct agacggcatg
     6181 aacaaacaag aagcaatcga taaaatgaat gaatggctag aagaaaatgg cgtagggaaa
     6241 aaagaagtca gctatcgctt acgtgactgg ctattctccc gtcaacgtta ctggggtgaa
     6301 ccaatcccaa tcatccattg ggaggacgga acagtcacag ctgtcccaga agaagagcta
     6361 ccattacggt tgcctaagac aaaaaacatc aagccaagcg gtactggaga atctccatta
     6421 gcaaatatcg aggagtgggt caatgtcgtt gatcctgtaa caggtaaaaa aggccgtcgc
     6481 gaaacgaata caatgccaca atgggcagga agctcatggt attacctgcg ttatatcgac
     6541 cctcataaca aaaaagaact agcagattat gaaaagttga agcgttggct accagttgac
     6601 atctacattg gtggagcaga acatgcggta cttcatttac tatatgcacg attctggcat
     6661 aaattcttat acgatatcgg tgttgttcct acaaaagaac cattccaaaa actatacaat
     6721 caaggaatga tcttaggcga aaacaacgaa aaaatgtcaa aatctcgtgg taatgtagtc
     6781 aatcctgatg atgtggtcga aacttacggt gcagatacac tgcgcatgta cgaaatgttc
     6841 atggggccat tagatgcttc gattgcttgg agcgaaaatg gcttagaagg aagccgtaaa
     6901 ttcttggatc gtgtatggcg tttgatcgtt gatgagaaca ataaaatgcg tgaccgtatc
     6961 actacattga atgatggcaa gcttgataaa gtgtatcatc aaacagtgaa aaaagtgaca
     7021 gaagactatg aaaatcttca tttcaataca gcaatctccc aattgatggt ctttatcaat
     7081 gaagcctata aagtggacgc tctgccttat gaatatatcg aaggatttgt tcaactactc
     7141 gcaccgattg ctcctcacat gggtgaagaa ctatgggcaa tccttggaaa tgatggtggt
     7201 atttcttacg caccatggcc aacatacgat gaagcagcac ttgttgaaga cgaagtagaa
     7261 gttgttttcc aagtaaacgg taaagttcgt gcgaaaagca atgttccccg agacttagga
     7321 aaagatgagc tagaaaaagc agcattagcc aatgaaatag tgcaagaata tattgaaggc
     7381 aaaacagtca gaaaagttat tgcggtacca aacaagttag tcaatatcgt tgctaattaa
     7441 ttggctcttt gtcaataagg actgatgagt tgtgcaaaat caaatctggg ccagaatgaa
     7501 cttcattctg tctcagattt tttgttattt caaattgatt aattgattga tcaaaaaggt
     7561 tttgattttc atgttctcaa acacgttcaa tgtctttttc ttctgaatgg tcttgtaaga
     7621 gat
//
LOCUS       DACDLI010000098         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDLI010000098 DACDLI010000000
VERSION     DACDLI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.098"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IV353_002443"
     CDS             complement(138..428)
                     /locus_tag="IV353_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010634.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IV353_002444"
     CDS             complement(546..806)
                     /locus_tag="IV353_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010635.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IV353_002445"
     CDS             complement(943..1356)
                     /locus_tag="IV353_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9010636.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IV353_002446"
     CDS             complement(1812..2297)
                     /locus_tag="IV353_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010637.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IV353_002447"
     CDS             complement(2436..2651)
                     /locus_tag="IV353_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9010638.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IV353_002448"
     CDS             2875..3675
                     /locus_tag="IV353_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9010639.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVAVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IV353_002449"
     CDS             3694..5562
                     /locus_tag="IV353_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9010640.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IV353_002450"
     CDS             5555..6046
                     /locus_tag="IV353_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9010641.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IV353_002451"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IV353_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9010642.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IV353_002452"
     CDS             6606..>7600
                     /locus_tag="IV353_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9010643.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtagctg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACDLI010000099         7357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDLI010000099 DACDLI010000000
VERSION     DACDLI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.099"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..512
                     /locus_tag="IV353_002453"
     CDS             <1..512
                     /locus_tag="IV353_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9010644.1"
                     /translation="KNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNKLD
                     EELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQL
                     KIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIEL
                     QISEGIDKQ"
     gene            588..806
                     /locus_tag="IV353_002454"
     CDS             588..806
                     /locus_tag="IV353_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010645.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            793..3471
                     /locus_tag="IV353_002455"
     CDS             793..3471
                     /locus_tag="IV353_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010646.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3462..3659
                     /locus_tag="IV353_002456"
     CDS             3462..3659
                     /locus_tag="IV353_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010647.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3663..4274
                     /gene="mobC"
                     /locus_tag="IV353_002457"
     CDS             3663..4274
                     /gene="mobC"
                     /locus_tag="IV353_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP9010648.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4259..5650
                     /locus_tag="IV353_002458"
     CDS             4259..5650
                     /locus_tag="IV353_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9010649.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5866..6180
                     /locus_tag="IV353_002459"
     CDS             5866..6180
                     /locus_tag="IV353_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010650.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6173..6667
                     /locus_tag="IV353_002460"
     CDS             6173..6667
                     /locus_tag="IV353_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010651.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6735..7049
                     /locus_tag="IV353_002461"
     CDS             6735..7049
                     /locus_tag="IV353_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010652.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7148..>7357
                     /locus_tag="IV353_002462"
     CDS             7148..>7357
                     /locus_tag="IV353_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9010653.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2821 a          994 c         1422 g         2120 t
ORIGIN      
        1 acaaaaacaa ttttgctgat agttcaaaaa atgaactaga attatataag tcttataaag
       61 atagtaaaga tattaaagaa tcacataatg atgatcttct tgaatcactt tcaactaaaa
      121 ataataaatt agatgaagaa ttgatcgaaa catttattga agaaaatctt cttaaacaaa
      181 tatggggaga aagtattgtg aattgcttaa aactggctag caatagtgat tatcgtcaat
      241 tcgacaactg gtacaaaaaa ttcaaatatg cgattagatc tgctgaaaaa gaacaaaaaa
      301 ttcaactgaa aattatctat gaaatttgta ataacaagta ttttgtagat catgttagag
      361 aacagctatc aatgacttta cgtgacttga tacgaagagc aaaaacagat catcgtatca
      421 aacaaaaaga cgattatatc tttgcttctc taaaaaataa agcgttagaa ctaattgaat
      481 tgcaaatcag cgaaggaata gataagcaat aagaatagaa ttgagtcaaa tgaaatggag
      541 gggtatctcc acttcatttg agaactttga atataggagg ggttattttg attaaaaatc
      601 gtttaataga tcaagtttgg tgtctagatc aaaatacaga tagtctcgaa ttaatcgaga
      661 ctatttgctt caacactata gttctcgatt taagagctga aaatgataat tgtgtaactc
      721 atgtcataat taatcaaaaa atggctcaga agctctctga gagcctcaga aaatgggcta
      781 agggaggtaa cgatgaaaat aattgaaaaa aagattgcca ataaaaaaat aaattggttg
      841 gttgttaatt ttcttgttct gatagctatg attattttta caagtaatat tgctcatgcg
      901 agtattttca attggtttga tgacacaaaa agtcaaacag agtttttgaa taattctctt
      961 tatagacctt acctaaaaaa gtacaatgga gatttcctta gtcagttcgg gatttggctc
     1021 ggatgggctg tggtaaaagg catgtttact gtgacagatt cgattcaaaa tatgattcca
     1081 gatgtgctgg atttgttcaa tttcattgaa agtacagggc taaataatgt ttatcaatca
     1141 gccgtaaata caattgtcat tggattaatg atcctttcat tgatgtttgt tggttacaaa
     1201 atgattactg gaaaaggaac agtggattta aaatcggttg gaatgaacgt agtgatgtct
     1261 atagcattaa tacttttaat gccaaccatg atttctagtg gtattcaatt tgctaaaaca
     1321 ttttataagg actctactac gattacaaat tcagatgacg gagtcgcttg gtcattgata
     1381 aaacaaggag taacagattt agcatatatt aataaaacag atcagtactc aagcattgat
     1441 aaaacggagg atcgaaataa tttaacacga aaaaattttc aacagacgga tctcacacaa
     1501 gtgttaacgg ataaagtgat tgataaattg gaaaaagaaa atccaattgc ggacaatctt
     1561 cgttatgaat tagttgaaaa ttctaataat gaatttgttg ctacaaagtt ttctgacaac
     1621 ttcttatcaa cattttctga tagcttgaaa tcaggctatt accgatacca agcaaatcta
     1681 tgggggattt caattgggtt gacagcgtta gcaatcgcct atgtgttcag cgcgtttgtg
     1741 atcattacag caatactaga acttgcattt aaacgtgtgt taggtgttct tgtttttgca
     1801 acagatattg agacaggaca gcgttcaaaa gttgttctat cagatattct acaatgttac
     1861 ttaactgttg ggtttcaagg atttgggtta tcaatgtttg ctatgtttat caattttctg
     1921 aactcaggac aaggtatctc aacaaatatt tttataaaaa caattgctta tatctgtgcc
     1981 gtttttgttt taatcaaagg cagtggaaca gtcatgagat actttggtgt tgatattgga
     2041 ctcaaagaag gctatgggca acttgcttct gcttttggaa tgggcgcaat gctgtttaga
     2101 aaaggatcaa atggatttaa tcgtgctaaa ggaagcggag aaggagaaga tcgaaaacca
     2161 gaaaagaatt tcggcgaaac tctatcaaaa aaagctggca aaactggaag agctttggga
     2221 tatgcccatg aacgtggatt atccggttta gcttcagatg gagctacaat ggctagtgaa
     2281 agagcaacaa aaccatttaa gagtatgcgt gatatagcta atgataccaa gaataagttt
     2341 aaagaaggtt tagatgatgg tacagtgtcc gctattaata aaaatagcaa acctatgtta
     2401 gctaaaaata aagaagatga aactggaaaa tatgctgatt ccgtgccaac acgtctttca
     2461 gatagaaaag atggtgctaa agttgaaaat gccgatagaa ttatgtctag ttctgaacgt
     2521 atgcgtgaag caatgaaaaa taatgctgaa tcgaataata atcctgcaag cgcaattcag
     2581 caaaaagtcc aacaagatat tgaagaacgt aaaaatgcta tgcacggtca agctaagtct
     2641 gctgaagaat taattaatca gaagcgtcat gaagcgaaat acacgcctga agcaatgaat
     2701 cgcgaggaaa taatgttaag gaaacgtgta gaagggcgga caggcgctaa tattgacgga
     2761 aaagaagcgt tgaccaaaga aagaattcaa gaagctaaaa attcaaatac aggattagaa
     2821 aaattagtca aagaaaatcc acaaaattct acaagtagtc atggtggccg atcagtagat
     2881 gtccgagaaa atattcaagg ttcactgaat ggtcatgttg gacggaatgt tgatgtaaga
     2941 gagaatttac aaagttcttc aaatgctcaa ggtactaaga caattgatgt tcgagaaaat
     3001 ctgcaaagta gcagtacaag tcaaccaaaa acagtagatg ttcgagaaaa tgtacaaaat
     3061 tctacaagta gtcaagggtt taaatcagtg gatgtccgag agaatgtgca aggtagcagt
     3121 gcaagtcaac caaaaacagt agatgtccga gaaaatgtac aaagttctac aagtagtcaa
     3181 ggattcaaaa cagtagatgt tcgggagaac attcaaaaag atagtggaga aatgaaagaa
     3241 aaaacacaaa aaatcaatat cgttgaagac agaaagtcag cgaaaaaatt tgattcaaat
     3301 catgaaacag tgattattga tagtgaaatc cgtgaaaatg ataagggatc aaaatcaaga
     3361 cgaagattca cttatgaaga caatgagtta ttcagagata cacttaatga tcctaatcca
     3421 cttttcgata atttattaag aaataaaagg gggggattgt catgcaatta attctaaaga
     3481 ttagtacgca tgatttctta gaacttgacg aattatgtga atcagtaaag aacgcaacaa
     3541 atgaaaaaat cacggtcact aattatattg aactgatgat tcaagaagaa ttaaagatga
     3601 aaaatgaaat agagagcaat ttcaaatgtt gctctctaga aaaaaatgtg aggggataat
     3661 caatggatga taaacaaaaa atgtacgaaa aaaatcgcaa acatagtgag gccttaaatt
     3721 ttcgtgtcga taaaaagact tatcttgaac tgacattgat tgcaaaacaa aggggaattt
     3781 ctagagcaga cgtttgccgt gagcggttaa agggactcaa atatgaaccg ccgttgttag
     3841 ctgaagatga tagggtagtt ttaaccaaac agttaaatca aattggtaac aatttgaatc
     3901 aagcggtaaa ggcattaaat atcattgcta aatattttag agaagaatct gaagataaaa
     3961 aagaaaagag cctacatttt agttttgatg ctagccgtta caacattttt aaagatgaac
     4021 ttgcgaatag catagcaaaa aagttgatga ttagttatcc agatgacatt tttttggagt
     4081 ccaatcggag tgccccctct ttttttaaat tatcagaagt agaaaaagaa tatttcgaat
     4141 attggataga aaagcaaact agaccgggta cgaaagaaga gtattcccaa aaagttgatc
     4201 ggaatttaat tcactcaatt cagattattg caagtttaaa agatgaggtg aaacaacttt
     4261 gggagctact ataacatcga aaggacgcaa gaaaaattta tcctcgctat tacaatatgc
     4321 agctaatcct tcaaaaaaag aaaaatgtgt tgcagtctca ggaataaatt gtagtgatga
     4381 tagtgaggaa gcactgcaag aaatgaattt agtacgtacc aagatgaata aatctaaaga
     4441 tatagcacaa gcgtttcatg ttattcattc gtttgataag agtacttcta aagagttaag
     4501 tattgataaa atgcacgaaa tagctgtcga atttgcagag aaagcttttc caaattcgca
     4561 aatcattgtt gcaagtcaca atgataaaga tcattttcat agtcatcttg tcattaataa
     4621 tattaacatg gaaacatgta aaaggttgcg tattgaccct aaagatttag aactaatgcg
     4681 aaaaattaac gatcaagtcc tcaatgaaca tggtcttgaa tcagtgaata atgagtacta
     4741 tgaaaactta gaaaagagaa ccaatcttta ttcaaacgat ccttctaaga tgaaatatgg
     4801 aaaaattaat tatcaagagt ttgtgcaaaa tgcgatcgaa gcaacgttgg aaaaatgcga
     4861 agtgaattct tttgaagatt tttcggatca tttggcaaaa gaatataaca ttgaagttta
     4921 tcaattttca aaaacaaata acaaacttgg ctatgttctt tacaaacaag gtgtaaattt
     4981 ctatgagcgt agaggtgagt taattacttt aggaagagat aaacaatcta gaaaagaaaa
     5041 aagtcagtat gtcgaacgta cttttagtgc aagaaagtta gggaaaagat attcatttga
     5101 atctgtttct gaacaacttg aaaagaattt agaagaatca catataaaaa atttttttaa
     5161 tatggaaaga gagcaagtta tgtcgctaat tagtggagta aattacaaaa ctggtgagtt
     5221 aaatgtaccg tttgtggaat tagattccat cgcacaaaag caatttagcg accgtgaagg
     5281 cgatgaaaaa ttcattaaat atgatgactt cttgcgagag gtagacaagc attttgtaaa
     5341 tacaatgtct ttagaagatg atcaagaata cacgattaaa cttcatacgg atgtgaatcc
     5401 tcaaacttta cgtagtgcga aaagtaaaac aatttctatt aaaaagactg atcagaaaga
     5461 gttatttcta ccggcaaagg gttttgaaaa ttgttctcat acaccttctg ctaaacagat
     5521 tcactatcaa atcagtcgca taatgaatga cgatgtaaaa atgtatcgtg ctaaaaagga
     5581 ttatgaattc acacaacaaa tgattgaaaa tcagcaaaaa aggcaacaaa attttgaaat
     5641 ggaattctga atgggcttgt tagtggactt tccagttttt tgcctatgaa gagttgcttg
     5701 gaaagaagca gtttttagga catacgccag ctacccatct acttgccagt caatccgccc
     5761 cgctcacgct aggctacttt ctggcaagtc agatggcggg taaatctctg agggagcaag
     5821 ctccctacag actccctccc aaaaaaagaa aggtggaaga aaaaaatgaa ctatccatat
     5881 tttaaagtct cggcttcaga agaaactaaa gaaatattca acaattttta caaccaaaac
     5941 aaaggaattt ttggttcaaa ggcaaatatg tttcgtgtta tggtttcaaa tttaccagtg
     6001 ttagctagtc catcaaataa taaatttaat gatcctgaaa gcattaaatt tgaacaaaaa
     6061 atttcagaac tagaatcaat gataagtaat gaagtaattg aaaagctgga tgacattgat
     6121 caaaagttat cgtattctct aaaaaacaaa tacaaaacgg aggagaaaaa agatgtttaa
     6181 agtttacttc ccgtttcagt ggctcgttaa aggaattctt ttagtgctac taggattgat
     6241 tctttttgga agttatctaa caattaaagc cagacatttt ccagaagaaa agccaataaa
     6301 aattgtaaca aaagatttaa aaataggtga aacttatcag gcagatgggg ttgaactaac
     6361 atttaaaaat attgaagtga ccgaaaaaag aaatgaattt gcacaacatg atcgtgtatt
     6421 acaagtgacc tatacactta taaatgatac agaaaatccg atacgtccac aacaatggtc
     6481 gattgaaaat aaggatggtg aagaacttta tgagtatgat atgatattgg ataatccaga
     6541 gtatattgaa agtggcaaag aagaaacagt aaattactat tatgaattac cgattgaaag
     6601 gcaggactac caaatcgtag tacgtagaga gaatggttac atagttgggt tgccatttac
     6661 tttataagtt ttattttgtt agtagttttt aaggtaatat cagttttgtg atttttgaaa
     6721 atggaactgg gagaatggct gaggaaaata aagttgttcc gtttgaaaca ttagtgataa
     6781 ataaggaaga gtttgagcaa ctttttcttg atttttttat tcaatatgac atgatttatg
     6841 attttagttc gtcactagat ttatttgaat tagaagcaga aataatagaa aacagtcgag
     6901 agaagaatgg taaaatcatt atttcagtag aaaaattcaa tgaattattc tcacaaacat
     6961 ttgaaataga tccagactat tatcaaccac aagaagtttt tcgagagttt agaaataaga
     7021 ttattggttt tagtcaaact acaaaatgaa agcttaataa agcaagtgtt gatagaatta
     7081 ttcctcaacg cttgctttat tttatgggca cttcataata gaggtataga gaagagaggt
     7141 attcattttg gatactatta ctccaacaaa cttcaaagaa aatataggta aagtgttgca
     7201 agaagtagtt cattcaaatt ctgaaataga aattactagg gattctaaaa atggattgaa
     7261 cgatggcgtc attatgattt ctaagagaaa atgggagttg ttacaagaag aactttattt
     7321 acaacgaacg ggtacatggg cttatgtatc tgattta
//
LOCUS       DACDLI010000100         6852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDLI010000100 DACDLI010000000
VERSION     DACDLI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.100"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            220..576
                     /locus_tag="IV353_002463"
     CDS             220..576
                     /locus_tag="IV353_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9010654.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            586..1485
                     /locus_tag="IV353_002464"
     CDS             586..1485
                     /locus_tag="IV353_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9010655.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2002..2733
                     /locus_tag="IV353_002465"
     CDS             2002..2733
                     /locus_tag="IV353_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9010656.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2878..4905
                     /locus_tag="IV353_002466"
     CDS             2878..4905
                     /locus_tag="IV353_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9010657.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5013..6110
                     /locus_tag="IV353_002467"
     CDS             5013..6110
                     /locus_tag="IV353_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9010658.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6417..>6852
                     /locus_tag="IV353_002468"
     CDS             6417..>6852
                     /locus_tag="IV353_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9010659.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT         2411 a         1104 c         1389 g         1948 t
ORIGIN      
        1 ttaagaaagg aataaggtta aacaaataac aggttgcttt ttgggtcttt ttccgttcaa
       61 gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat cataataaac
      121 gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta gttgcatctg
      181 atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga agtgacaaaa
      241 gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt attagatatc
      301 gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga tacggcattt
      361 cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc tgtagtggct
      421 gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat ggatgatcca
      481 tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc aagcggcttg
      541 agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg aaaattggcg
      601 atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct gatgcagctt
      661 tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga tacggctaat
      721 aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt atctacgaca
      781 tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat ggaccatttt
      841 ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac cgttctttta
      901 gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga ggcaatcgta
      961 cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac agatctcgag
     1021 gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga agcaaaaaaa
     1081 cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat gtatcaatct
     1141 gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc attcggagaa
     1201 ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt
     1261 gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta ttattatgaa
     1321 tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta tgatcctatg
     1381 aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca aacacaacat
     1441 ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag cattagtaat
     1501 gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga tccatttatc
     1561 gtggatatgc tgcttttttt gcggaaaata atcagcgaat gtttatttga tcggatgatt
     1621 tgtaactcgc agagtactag atgcaagcgt gtattctttt tttgaacgat aatcacaaaa
     1681 aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag tctaggcagt
     1741 caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg ctatgacagc
     1801 tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg
     1861 ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt cttttatttc
     1921 ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat atgtttagta
     1981 aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc agcaatgatg
     2041 ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata cccaaaggaa
     2101 tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac tcctcctgtt
     2161 gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc aagccctgga
     2221 acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac tcaaaaaatt
     2281 tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa
     2341 ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa attaagtgtc
     2401 tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc acagctgaaa
     2461 tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca aacggtgaat
     2521 tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc taaatcagga
     2581 acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg
     2641 gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc cactaaatta
     2701 gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg ttaaaaataa
     2761 atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa
     2821 tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa agatatattg
     2881 aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat ctttccgatc
     2941 atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga cgaagaaagc
     3001 aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga aagcagtgag
     3061 gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga gggttcggac
     3121 aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga tcccaatatt
     3181 atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat attggaaaat
     3241 gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat ttggtcaaat
     3301 aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt aggaaatcaa
     3361 cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag aatggctagt
     3421 aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc tactcgttct
     3481 ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa aaatagtggt
     3541 tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg acatgtcgct
     3601 tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg cgtgacggta
     3661 gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg ggacgcggag
     3721 caaagaagtc ttacttatga tttggaaggt gtcggtcact cttctcggat tatacctgat
     3781 ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc aacaggtact
     3841 ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac atcaacagct
     3901 gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg aaacgataag
     3961 ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa gaaaatgcag
     4021 actagtatcg acctcccaca agaattaact attcccgagc atatcttgat caatggaaat
     4081 caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa tctgacagat
     4141 tattttattg ctggagatac gttaataatc gaattggaga caactttgca ggaagtaatt
     4201 cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt gattgagcaa
     4261 tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca tgtttattat
     4321 aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg gaaaatagga
     4381 gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc ggattcagcg
     4441 aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta cagaaaaggc
     4501 aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa aatatccagt
     4561 agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga tgaacgggct
     4621 tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg agattttgag
     4681 cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg gacatccgct
     4741 gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct tttagattct
     4801 acagctcatc gtggaatcag aatagatatt cctgtagaaa accaaagaat cggaaccttt
     4861 gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga agagaagaat
     4921 ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg tgtcccacag
     4981 agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt ttcttgagac
     5041 caaaacaaca attatttata acactcctag ttattttagg ggcacttttt aacaatatat
     5101 cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag aatcaaatcg
     5161 acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta gaagtaaaag
     5221 cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa
     5281 ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc
     5341 tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta aaacctaaaa
     5401 gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat ggtgtattgg
     5461 ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa actaaggatc
     5521 aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg caacagacac
     5581 tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa atcaatgcaa
     5641 gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat caagtagtta
     5701 ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag gaaaaagaag
     5761 gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat ggtcaaccgc
     5821 taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat gaaaatggaa
     5881 aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg gtatttgaaa
     5941 aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat gttacgatta
     6001 aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg tttattttat
     6061 tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata
     6121 acaaatagta aatttttatt attgtaggat ggttgggctc tttttataac aatataaaat
     6181 ataccatttt attaataaaa atgcatacta gtaaatgata taattctaat aacaggtttg
     6241 atcgcgagaa aggccaaata aaacgctctc caaaatgtta aaaaaaagac aggacgatgt
     6301 atattataca aacaagaatc gctgacaaca gtgactcgga tccaggaaat cagttttatc
     6361 cccagctagt tggtgaatgc atggaaagta atatacgtag aaagagctga tgactaatga
     6421 attatgagtt ttttttaagc aaagaagatc acagaagtta ttcattactt aaatatttag
     6481 aagcaagctc aaatttatca gagtcaatat cgaatgttca agaagagttg tcgctatcga
     6541 catttctctt gaaaaaaacg atcgataagc tgaaacatga tttacaaaag ttcgatctag
     6601 agaaaaattt gcgagtaacc gtcagtgaat tggatatatg tctggaaatt aatggaaaat
     6661 gttctagcaa agctctgtta tctcgttata taacggattc tttatctatg aaaatggtat
     6721 tggctttctt tcaggaaaaa tatatctcag tagaaaattt tgctaaacag catcacgtga
     6781 gttattctgt ggcttataaa gtattacaaa gactaaaaag aaatctaaaa aaatatcaaa
     6841 ttttttttga aa
//
LOCUS       DACDLI010000101         6755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDLI010000101 DACDLI010000000
VERSION     DACDLI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.101"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IV353_002469"
     CDS             690..1658
                     /locus_tag="IV353_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9010660.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IV353_002470"
     CDS             1669..2484
                     /locus_tag="IV353_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9010661.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IV353_002471"
     CDS             2552..3262
                     /locus_tag="IV353_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9010662.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..4797
                     /locus_tag="IV353_002472"
     CDS             3298..4797
                     /locus_tag="IV353_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9010663.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNVSTEDFFNYCRLEESG
                     VTVTSGTDLPLFITDIPESIIRAVFRRFPEGEEVWEKETSFTTTELLKSFTSNSFKVN
                     GLSEYGEIKVGKSASLAVFSEDLCTDDWQKVMAAEVIATYLDGDLVYKK"
     gene            4845..6245
                     /gene="yfcC"
                     /locus_tag="IV353_002473"
     CDS             4845..6245
                     /gene="yfcC"
                     /locus_tag="IV353_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9010664.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            6278..6445
                     /locus_tag="IV353_002474"
     CDS             6278..6445
                     /locus_tag="IV353_002474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010665.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            6499..6755
                     /locus_tag="IV353_002475"
                     /pseudo
     CDS             6499..6755
                     /locus_tag="IV353_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         2164 a         1043 c         1420 g         2128 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaatgtttc cacagaggac
     4441 ttctttaatt attgtcgatt agaggaaagt ggtgtaacag tcactagcgg tacagattta
     4501 ccgctgttta ttactgatat tcctgaatcg attataagag cagtattccg tcgttttcct
     4561 gaaggagaag aagtctggga gaaagaaaca agcttcacga caaccgagct gctgaaaagt
     4621 tttacaagca acagcttcaa agtaaatggt ttatcggagt acggagaaat aaaagtaggg
     4681 aaaagcgcca gcttagctgt tttcagcgag gacctctgta ctgatgactg gcaaaaagtg
     4741 atggctgcgg aagttatcgc tacctatctt gatggtgact tagtttacaa gaaataatgc
     4801 ttttcgatat aacgttataa aacgaattga agagaggtta aaaaatggaa acaatgaaaa
     4861 gaaaattcac cttcccacat agttatacat tgatttttat cttaattatt ttggctgcaa
     4921 ttttaacttg gataattcct agtggtcagt ttgagacagt tgttgaaaac gtcgcaggta
     4981 tggaaaaaac aacggttgtt ccgggaacct atcaccaaat cgataaggca gattatggac
     5041 aaggtttttc ctctatacta atggcaccag ctgaagggat tatttcagct gttgaggttg
     5101 ttgcttttgt tctcatcgtt ggtggtgcct tcgggattat cctaaaaacg ggagccatcg
     5161 atcgagggct gtttacctta gcaaaagttc tgggagacaa ggggatctta gttattccga
     5221 tgtcgatgat tctattcagc ttaggaggat caacctttgg catgagcgag gaagtcattc
     5281 ccttatttgc ggtgtttatt tctctgatgt tttctcttgg ctatgattcc atgactgcga
     5341 tcttgattct atttttaagt acacaagcgg gatatgttgg gtcgacgatt aatcccttca
     5401 acgttttaat cgcacaggga gtagctggcg tttacggaaa tcccctgctt tggtttcgga
     5461 tgctatcctg gattgtcatt actgggattt caattgtttt caccatgctt tatgctagca
     5521 gagtcaaaaa gaatccactg tcctctattg tttatgagag tgatttaaag gttaagcata
     5581 agtttcaaaa ttttgacact gaaaaagagt tttcaacaca agataaacta attatcgccg
     5641 gttttatcgt aggtttagga atcatgattt ggggtatcat ttccaaggga tggtatatgg
     5701 ttgaaatttc tgcactattc ttaggattag gtctttttgc tgggggcatc gctagatttt
     5761 ctcaaaagga aattgctgaa gcttttgtcg aaggctgttc agatttcgcc tatgctgctg
     5821 taatcattgg gctagctaga ggagtcttgg taatcttgga aggtggaatg attattgaca
     5881 ctattctaaa cagcttggct aaccttttag gtggtcttcc aaaattcctg ttcactacga
     5941 ttctactcat ttctcaagta attgtttcct tttttgttcc atcctcctca ggagctgcgg
     6001 cattaacgat gccggttatg gcaccattgg gtgatctaat gaatgtaagt cgtgatgttg
     6061 ttgttctaag taaccagttt ggaaatggtt taatgaatat tatctcccca actagtggtg
     6121 tattactggc aggattagcc atcgcagaga ttaatttctc taaatggttt aaggttggct
     6181 tcaaaatatt tgccatctta acccttgttt ctgctgtttt gttatacatt gctacactct
     6241 tttagtctta atcatccatt aataggaggc aatagttatg attaaaataa atcaattagc
     6301 aggaatgaat atccactatt tattttattc tctcaaagaa tttttcgctt atcaacagaa
     6361 aattggtatt tcttctgttg aactttgggg aggtgctccg cattttttca tagatgcttt
     6421 tactggttct gttgcaaagt tttaaatcta ctatcaaata aggtagaata atagaaaaag
     6481 atagcaggag gaatgacgat gaatcatttt aaaggaaagc aatttcagca ggatgtgatt
     6541 attgtagccg tgggctacta tcttcgttat aaccttagct atcgtgaagt tcgagaaatc
     6601 ttatatgatc gtggcattaa cgtttctcat acgacgattt atcgttgggt gtaagaatat
     6661 ggcaaactac tctatcaaat ttggaaaaag aaaaataaaa aatcctttta ttcatggaaa
     6721 atggatgaaa cgtacatcaa aattaaagga aaatg
//
LOCUS       DACDLI010000102         6711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDLI010000102 DACDLI010000000
VERSION     DACDLI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.102"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            98..1651
                     /locus_tag="IV353_002476"
     CDS             98..1651
                     /locus_tag="IV353_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9010666.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2032..3816)
                     /locus_tag="IV353_002477"
     CDS             complement(2032..3816)
                     /locus_tag="IV353_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9010667.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(3831..5552)
                     /locus_tag="IV353_002478"
     CDS             complement(3831..5552)
                     /locus_tag="IV353_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9010668.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5652..6413
                     /locus_tag="IV353_002479"
     CDS             5652..6413
                     /locus_tag="IV353_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9010669.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         2121 a         1200 c         1314 g         2076 t
ORIGIN      
        1 agagtacata ccaaattcat aaatttttta ttagtgggac tagtgattgt atggattttg
       61 acgcataatg aatgaagtat gagaaaagga gagacaaatg gatcaagaac tatttattat
      121 cgaagtatta tcagctaaac aaaaaattat cgagaaagcg aaaaaaagag cgaaaagaat
      181 aatgttcaag cttttaagct tagtattttt ctctggtttg ctttttttcg gagctgtatc
      241 gttcctatat cgccatatga gtgcagtagt ccgttcagct gaagggatgc aaaaattaaa
      301 taatttgtat cacttttcgt ggggagttgg cggaataaca attgttgtgc ttattgtttt
      361 tcttctactt atttggctcc gttaccatca attgtcgcgt catttcgatc aatggcttag
      421 tcaaagagca caacagtttt tacataataa accaacttct caagatgatg actatcttta
      481 ttttgagaag acttcgacga gtcaagcttt tcggatacaa aaaagtgatt gccagttatt
      541 gctgagatca cttgatgatt tacctatcta cttaggcgaa agcaaatcaa aattcggaat
      601 gaacacattc catatttttt tactagaaaa cgaacccaga tgggaaaaaa gccagtcttc
      661 gcctggtgga tacctgacaa ctaagatgag tacattagtc actggttttt tcctcattct
      721 tttagctgga acaatctatt atgggagata cactcagcca aaaatgtctt ctcatataaa
      781 tgctgcttct cacatgacaa tgaactcaac taacgatacg catatacaaa agacaaatga
      841 agggttgtta gctgatccta agcaaaaggt aaatttgaca acacaaaaga taaatgatct
      901 caaacttgat acagagacgc actctctcta tatgacaaca gatagtggtg ctaattgggt
      961 atttgtgccg attgattcgc aatggttgcg ttttggcgaa tacaccctga caacggggct
     1021 gattcctgaa ggttattgga tggacaacac atatgatatt tccgccgatt tctcatggtt
     1081 tatctattct tctggggatg atgtacgtct gctgacttca agagataatg gtaaaacatg
     1141 gcaaacgtct attatcaccc aatcagttca gccgatacgt tatcgaaaag cggtatttta
     1201 tggcaacgat ggtctattta tctgtacatc agccagtggg atgtctgcag aaactttgta
     1261 catttacact actatggatg gcggaatcac ttggataaaa agtggaagta caatgatcaa
     1321 tcagccagta caaaatgtca gcttcgttac aacttcttta ggttttgttt cgacacgtga
     1381 aagtttgttt tatacaaaca acggaggacg ctcattcaaa gaatcagttc tgtcgattcc
     1441 ggaaaactat cagacagggg ggatagatct ttttcaatca ccaaatgaag ttactcaaat
     1501 aagcgcgaat acgttagaga caaaatttta tttgttgaaa aaaggaccaa tcgatgcagg
     1561 gaaaatgttt gcctgcctct tccgttcaac tgataacggg gaaacatggc aattcgttca
     1621 gcaactatcg caagtagaac aaggagatta aagaagagag attgtgagcc tgacgttcag
     1681 caccgagtca caagaaacaa attcaaaaaa cagcttctca tatttgttga atagtgtaac
     1741 tcgtgattcg gagttgacaa gagcaacaag aagcgatttt gaagtatagc ttctcgtatt
     1801 ttacaagatc gtgtcttatt gccgataggt tgtctcttga acaccgttta ttcgtaaaaa
     1861 gggattgtga caagtctttt gtcactactt accgaacgaa actgaaggag ctatccaaaa
     1921 agtatcatta gtattattct ttgtgtttct agtggcacca ctagtacatt taaaaaagct
     1981 atcgatcatg gactaatcat acatgatcga tagcttttta gataatgttt gttacgctat
     2041 ctgaaattga ctattataaa gactggcata aagcgttggt gattggagta gttcagaatg
     2101 tgttcctttt tcgacgatat cgccattctt cattacaagg atcatgtctg cattttcaat
     2161 cgttgacaaa cggtgtgcaa tcacaaaact tgtcctatct tttgtcacag cttccattgc
     2221 ttgctgaatc aatgcttctg ttcgtgtatc tacgctagaa gttgcttcat ctaaaataac
     2281 gacaggagga ttcgctagaa tgactcgagc aattgtcaat aattgttgct gtccttgaga
     2341 taacgtgccg ttttcgctag aaatgatcgt atcatatccg tctggcagtg ttcgaataaa
     2401 gtgatcacat tgtgcgattt cagccgcttc gataatttgt tctcttgtgg catccatttt
     2461 cccgtaggcg atattatcag ctaccgttcc ttcaaacagc caggtatttt gtaaaaccat
     2521 cccaaaagaa cgacgtagtt ctccacgtga aagcttggta atatcaagac cgtcgaattc
     2581 gatctttcct ttattcagtt cataaaaacg catcagcaga ttgactaaag tcgtttttcc
     2641 tgcaccagtt ggaccaacga tcgctaccgt ttgttttggc tttactgaaa aatcgacttg
     2701 attcatcaat attttatctg atgtgtaacc aaactgtaca tttttgaaag ctatttttcc
     2761 tttcggttgt tctaaaagtc ttgcatctgg aaggtctgga atttcatctg tttcatctaa
     2821 tatctcaaat atcctatcga ttgctgccat tgcggattgg atcgagttga tcacataaga
     2881 agcggtagaa attggttcag aaatctgatt gatgtactga agataagctt gtaagaagcc
     2941 gatcgtcaca ccacctgata gaacaagcat agcaccgaat accgcactta tgatgaaggc
     3001 gagttgatta atgaaacgaa tggccggata gatagcacag ttcataaatt gagcacgttt
     3061 aaaagcgttg tattgctttt tatttacttc gtgtatagca gcttctgcat gagcttgctg
     3121 attgaatgtt ttgaccacta gattaccagc taagatttct tccattcgat tatttaaaac
     3181 acccagttca tactgattct gttcagaaat ctgtttgttt ttgttcgcaa tcttagttgt
     3241 aacaattgaa ctgccaacga tcagcaataa gacgagcaaa gtaagcttgc tttcgatata
     3301 aaacagcatc agaccagcag cagctattgt tacgacagaa gtaaaaaatt gattgatacc
     3361 tgtcaataac acttgagaaa gttgattgag accggtagtt gtccgactaa tgatgtcacc
     3421 tacttgatga ttgtcaaaaa acgacatcgg taatgttttg aatttttttg tgatttcttt
     3481 tcgcaccctc aatgtgatct tttcgcttaa cgctgccatt gtccgttcct gaataaatga
     3541 agtgatgctg cttaagactg aacagccgat cagcaataag acagggaaca acaatgcttg
     3601 tttgatatca ctgaaagaaa gctgtccaat acctgttttt cttatttctg ataagagatc
     3661 atcgatcccg attcccatga tcaagggcat ggctacgact aataggttcc cgaataaact
     3721 gcatagaaac aatccgtaaa aatatttatg ttcaggtttg atcattttcc aaaaacgtat
     3781 caaagcacgc gtgttcaacg tttttttctt ttccatcgta tcccttcttt ctatattcct
     3841 tgtgattttg caaactcctg ataagtttgg caattagaca gtagttcttc atgagtacct
     3901 tgtccaacga tttttccttc atctaaaaca atgatgttat ctgcttgttg gattgtactg
     3961 aggcgttgag caacaatcac gagagttttc tccgacatgt gtttatgaag ggctgctctt
     4021 aatgctgcat ctgttttata gtctaaagcg gagaaactat catcgaatac atagatctct
     4081 gctggtttga tcaacgctcg agcaatacat atccgctgtt tctggccccc tgaataatta
     4141 gaaccgcctt gagcaacaaa gctgtccaat ccgtcaggaa gggtgtccac tagttcttct
     4201 aattgagcga tcttgatcgc tttagacaac tcttctgttg aggcatattc atttcccatc
     4261 tgcaagttgc ttaaaattgt cccgctgaat aagaaagcct tctgagggac ataactgatg
     4321 tgggagcgga tctgttcttg agtcatttga gtaagaggaa taccaccaag ggttactttt
     4381 cctatcgtag ggtcactaag tctcaagaga agtttcgcca aactactttt tccagatccg
     4441 gtcccgccaa cgatcgctgt tgtttttcct ttaggtattg tgaaatcaat atcttctaga
     4501 actggatcag agatttcagt atatttaaat gtgacatgtt caaaagcagc tgcatttgtc
     4561 tgatcaaagg ttggttcagc tactgttttg atcgtctctt cctcaatttc gatctctgct
     4621 tgcagtattt cttctatccg tcgtaatgag gcaaacgatt tagggataat gaccaatacc
     4681 atagctgcga tcatcaagta agcaagtgtc agcatagagt attcgactac agcagtcacg
     4741 acaccgattt gtaatgctcc ttctgctaca agatttccgc caaaccacaa aatagaagca
     4801 taaacgattc ccatgagcaa aaaagcaatt ggagtgaccc aagcaaatat tcggttcact
     4861 ttgatcattt gattagcata gtcagcaaag ctcttattcg ttcgcttttc ttcatatgat
     4921 tgattatcaa aggcacggat catattaatc ccagtaaaga attctctcaa tgtgatggtg
     4981 atccgatcca tttttggctg gatactcaaa gataaaggtg cccctcgttt catcagtcga
     5041 taaacaatcc atacataaaa aacaacagag atcaatgtaa cccatgttag taatagagaa
     5101 taacgaaaca tcatgagaat ggtgaaaacg caaataaccg gtgctggtaa gatcatttgg
     5161 caaaataaaa caatcatttg ctggacatta tcgacatcat tcgtcattct tgtcaaaaga
     5221 gatccagaac taaaacgact gatatccttc attgacagct gttggaattt acggaaatat
     5281 ttctcccgcg tttggaatcc aaacctagcg gaagccaatg ctgaaaagta actagaggca
     5341 actgcagcga ttgttcctag aaacgctaca agcagcattt caactccaat tttttttata
     5401 actgaggctt cgccacttgc tatacctgtg tcgatcagct ttgctactaa ttttggtaca
     5461 cctaatgtac cggcgatttc taaacagatc atcacaaagg taaggagagc gagtcctttg
     5521 tttttctgta agaatgtttt taagatagac atattattcc tccttttttc ctgatacttt
     5581 tcgattctat accttcaagt aacttgaagg tcaatagaaa aaagaataaa ctgaaaaaga
     5641 ggtgagaaaa aatgaaaaaa atgtatacga ttggccaagt atcggaacta tttgatttgc
     5701 caaaatcaac gatccgctat tgggatgaac aaggattaat ccgttcaagt agacaagaag
     5761 agaatgatta tcgattgttt gatatcgatg atatttttat gctttatgat atcgtttttt
     5821 atagaaaatt agatataccg atcaaacaga tgaagaattt atatggaaaa acattgacgg
     5881 agttatatga gacacttgat gaaacagaaa gaagaatcca taaagaactg gtggtgatga
     5941 aacaaaaaca aaaagagata agggagcaaa aaaaacagtt gaaattgatg atcgatacaa
     6001 acgaggaaga atttcctgta gaagagatcc cttttgactg tatgatctca acggagtttg
     6061 aagatattgt ggaaatcaaa aaatttctac ctaactattc aagctttggg atgatgagta
     6121 tgccagcttc cagttcacag acgatgtatg gattttttat cgaccctagt gaagtacatt
     6181 tatttactca agacgtgatt tgggagaaaa aagatacggc tgtttatcgt cgattcttat
     6241 tgaaatccga gatgaaccat gcggaacgta ataatatttt agaaatcaga gaaagaatgt
     6301 atgaaaaagg atggaaaaca ggagaagtga tcggccagta tctattgacg aatacggatg
     6361 aaaacaatat cagaactgaa tattatcatg catggataga aatgaaaaaa taggaaatat
     6421 gtgtttaaga taactcgaaa atgatggttg ttttagttat aaagaaaaat tttctggttc
     6481 gattacgggt ttaatgtggg ggattattag tcgtggatag atagaatttt agttttttag
     6541 aggaaatatg tctttaaaaa tggaatggtt gcaattctta gcgatatata cgatgtaatt
     6601 gtcctcttaa aaaagtatgg aaaaataatt gtattgggtg ttatgtataa ttcatggtga
     6661 actgagacta atgagaagaa aaattcatat tgttatgagt gagattgtat c
//
LOCUS       DACDLI010000103         6707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDLI010000103 DACDLI010000000
VERSION     DACDLI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.103"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..241)
                     /locus_tag="IV353_002480"
     CDS             complement(<1..241)
                     /locus_tag="IV353_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9010670.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYI"
     gene            544..1818
                     /locus_tag="IV353_002481"
     CDS             544..1818
                     /locus_tag="IV353_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9010671.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1872..3299
                     /locus_tag="IV353_002482"
     CDS             1872..3299
                     /locus_tag="IV353_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9010672.1"
                     /translation="MTKKAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            3312..4184
                     /locus_tag="IV353_002483"
     CDS             3312..4184
                     /locus_tag="IV353_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9010673.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            4279..5274
                     /locus_tag="IV353_002484"
     CDS             4279..5274
                     /locus_tag="IV353_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9010674.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            5980..6597
                     /locus_tag="IV353_002485"
     CDS             5980..6597
                     /locus_tag="IV353_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9010675.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTPKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT         2318 a          933 c         1296 g         2160 t
ORIGIN      
        1 caatgtattc tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat
       61 tttctttgta ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga
      121 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt
      181 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      241 tttatgttca ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg
      301 gcttttttat ttgtctcgct taattataca aacggcgtta taaaaaatag tgccattaaa
      361 aaagaaaagc gacacaccaa taaaagtcat atcctgtgac attatatggc attatccaaa
      421 tagaataacc ggttgacatg taagcggatt tcttttataa ttttaggagt acaagtcctt
      481 ttctaaaaat gttaaccggt tatcatgaaa ggggatacaa atatatcgtt ggaggataaa
      541 aatatgtcaa aagttaacgt atttaaaaat caatcttatt tacaaagttc agctacatta
      601 ttactatttt ttgcttcttg gggtgtttgg tggtcatttt ttcaactttg gctaacatct
      661 gaatcaaatg gtttagggtt atctggcagt gctgtaggaa cagtattctc ggcaaattcg
      721 ttagttacct taattttgat gtttatttat ggaacattac aagataaatt gtatattaaa
      781 cgaaatttat taatttttgc ttctgtatta gcgacacttg ttggaccatt ttttatatgg
      841 atatatgggc cattgctaga taacaatttt aatttaggca ttattatggg agcgctattt
      901 ttgtcagctg gatatttagc ttctgtagga gtttttgaag ctgtgtcaga aaggtttagt
      961 cgtttatttg gctttgaata tggacaagca agggcgtggg gatcatttgg ttatgccttg
     1021 gtagcgcttt tggcaggatt tttatttgta aaaaatcctc atttaaactt ttgggcggga
     1081 tctttctttg gttctttact attgttaaat ttattatttt ggaaccctaa agttgaacgg
     1141 gaagcaaatc aaaattttaa tcaagaacaa gctgaatcaa atagtattcc ttctttaaaa
     1201 gaaatgtttg atctaatgaa actgcctcaa ttatggacga taatcatctt tattgttttt
     1261 acatggacat tttatacggt attcgatcaa caaatgtttc cgggatttta tactggtttg
     1321 ttttcaacat cagctaatgg tgaaaaaata tatgggacat tgaatgctat tcaagtattt
     1381 tgtgaagcgt taatgatggg aattgttcca atcattatga gaaaattagg ggttcgaaat
     1441 actttgttat taggtgtaac cattatgtgt gtacgaattg gattgtgcgg gtttgcctcg
     1501 acaccattat ctgtttcatg cataaaaatg ttgcatgctt tagaagtacc attatttaca
     1561 ttaccaatgt ttcgctattt tacacttcat tttgatacaa agctatcagc aaccctctat
     1621 atgataggat ttcagatagc tgctcaaatt gggcaagtga ttttatcaac accattggga
     1681 atattaagag acaacgttgg ctatcaacca acatttaaaa ttatttctct tattgtatta
     1741 ctagcaggca tatatgcatt ctttattctt aaacaagatg atagagatgt tcaaggggat
     1801 ccatttattc gaggataatg gaaaccaaca gaataaaaaa caattttatc tagaaagtga
     1861 gcgaatatat tatgacaaaa aaagctatag aattaacaaa tacacgttat cgtttgggat
     1921 atcatgtgtc tgcaccatca ggatggatta atgatccaaa tggattttgc tattttgatg
     1981 ggtattatca tgtgttttat caacatcatc catatagtgc tgaatgggga ccaatgcact
     2041 gggcacatgc tagaagtaag gatttagttc attgggaatc tttaccactt gctttgactc
     2101 ctggagatca agaagatgaa ggtggttgtt tttcaggaag cgctattgaa aaaaatggtg
     2161 tgttgtatct gttctataca ggacatcact attatggaga caatgatcca gatcattttt
     2221 ggcaaaatca aaacatggcg tatagtacgg atggcattca ctttactaaa tatgaaaaaa
     2281 atcctgtaat agcaaaagca ccagaagata acacacatca tttcagagat ccaaaagtat
     2341 ggaaacacga tgaaaaatat tacatggttt tgggtagtca agaaaaagat ggattaggtc
     2401 gtgctattgt ttatcagtcg ttagatttat taaattggga ttacattgga ccaatttcta
     2461 aagcgaagga agtacttaca gaaggattta tgtgggaatg cccagacttt tttgaattaa
     2521 atggaaaatc tattctttta ttatcccctc agggaattga agcaaatgga aaagattatt
     2581 taaatctata tgaaacaggc tactttatcg gtgattatga ttatgagaca gcaaaattca
     2641 ttagaggaaa tttttatgaa ttagataagg gacacgattt ctatgcaaca caaacaactt
     2701 tatctccaga tggtagacgt attgttatgg cctggatgga tatgtgggaa agccctatgc
     2761 ctgaaaaagt agacggatgg gcaggagcat taacgcttcc tcgagaatta gagttaattg
     2821 gagatcgttt gtatatgaaa cctgtgagag aattagaaca acttcgagtt ggcagcggcg
     2881 ttgaaacgac agttaatttg gcaggagaaa taataatcgc tcaagatgct tcattcaaag
     2941 aagctctaat tgatgtacta ttgactaaat caaaaaatca agaaatcaaa ttttcattaa
     3001 aaacaaaaga tgaagagtta atcaatattt tttattctaa ggataaaaat gaatgtacct
     3061 taactcgtaa cggcaaaaat gatccaaggt atggaaccat tcagatgtgt gatagattat
     3121 cacttcgagt gtttatcgat aaaagttcgg cagagatttt tattaatgat ggcgagcttg
     3181 tttttacaga gcgttattat acagaaaatc aaccaagtat gcatttgagt gttagtgaag
     3241 aagcttttgt ctcgagtaca atttatcatc taaaaaaaga tgcaatttct tacgagtaag
     3301 gaggttttat gatgttaatt ggtagcatag aagcaggtgg gactaaattt gtatgtgccg
     3361 tgggagacga ggattaccga gtacaagacc aaataatttg tccaacgacc acccccaggg
     3421 aaacattgaa aaaaactgtt gactatttta aacagtttga tataaatgca ttaggtattg
     3481 catcatttgg tccaatagaa attcgaaaaa attcaccaaa atcaggatat attacgtcta
     3541 caccaaaggt gggatggcga aatgttaatg tgttaggtta tttacaagat gagctgaatg
     3601 ttccaatgtc ttggacaacg gatgtgaacg gatcagctta tggagagtac atcatgtcca
     3661 ctttatcgaa tgaaaaaata aattcattag tttattacac cgttggtact ggcgttggtg
     3721 gtggagctgt tgttgacgga aaactattag gatcgtttgg tcattcagaa atgggccata
     3781 cgtttgtaaa acgccatcct gatgatattg attttaaagg aatttgtcct tatcatggtg
     3841 attgtttaga aggcttagtt gcgggaccaa cgtttgaagc acgttttgga aaaaagggtg
     3901 aagacgtttc tttaacggat cctatttggg atgtaatggc ctattatcta gcacaagctg
     3961 tgattcaaac aacattgatt atacgaccgg ataaaatagt atttggtgga agcgtagtaa
     4021 atgaaaaatt gttagaaaaa attcgtgtta atgttaataa attattggat gattacgtgg
     4081 aaataccacc gttaagtaaa tatattacgc gtcctttaat taaaaataat ggatcggcaa
     4141 cactaggaaa ctttgctttg ggcttgaatc aattacaaga ataaaacata gaaaaaatat
     4201 cgattatcag gtatagtagt tctatagagt tctttttttc gatagttaat tattgcatta
     4261 tttaggagag acaagagaat gcgcccaaaa ttagaagatg ttgccaaaag agcaaatgta
     4321 tcaaaaacaa ctgtttcacg tgtattaaac aatcgtggct atttaagtca aaaaacgatt
     4381 gataatgttt ataaggctat agaagaactc aattatcaac cgaacgttgt agcgcgtcaa
     4441 ttatttcaaa agaaaactaa cattgtcggg ttactatttc ctacagtagc caatcctttt
     4501 tttagtgaac tcgttgaggc attggaaaaa aagttatatg aaattggtta taaggtatta
     4561 ataggcaatt cgatgaataa taaagaaaag gaaacaaatt atttaaatca gttattgtct
     4621 gatcaagtag atggtttaat tgttggtaca cataaccaag gaattcagga atataaatat
     4681 cagaatttac cgattgttgc aattgatcga gtgatgaatg aagatattcc agttgttgaa
     4741 tccgataact acaatggtgg aaaacttgca accaagttat taattgcaca aggtgcaaaa
     4801 aatattattc atacaaacgg tccaattgac ttacagactc cggctaatcg tcggagacta
     4861 gcctatgaag atactatgaa agcatatcag ttaattccac gaactgtgac attagatttc
     4921 aatatttctt atgtaaagaa aaaacagatt tttttccaaa tgtttgagga ttatcctaaa
     4981 atagatggta tttttgcttc aaatgacatc gatgcggccc taatattaca agtagcaaaa
     5041 gaaaaggggt taaatgtacc agctgattta ttagtagtcg gatatgatgg tactttaatg
     5101 actcggagta tattaccaga tctgacaaca gtaatacaac caattaatga tatagcagac
     5161 acagctgttg ctattttgat gaaacgaata aataaagaag aaacaaaaaa ggaatatatt
     5221 ttacctgtca cattatggat ggggaagaca tcagtaaaga aaaagaacaa ttaaatttat
     5281 aaagactatc aatgataatt tcattattaa ttcgtgatat aaaaaattgt taaatctctt
     5341 ataatagact atgatacaaa atcatgcttt taatctgtgt agatgttgcc aaatatttga
     5401 aagacataga acttgtcaga aaaaccttaa caattcctca atgggcggat aagctaggca
     5461 aaagggctgg cataaatttt tcagtccttc taacagaatc aattgcagat aaagcagata
     5521 acattcttcg ttctggaaga aataattaga actcaaaagg gtcagaaact taaaagtttt
     5581 tgatcccttt tttattcctc atttcatagc ttttttattc ctcatttcat agctttttta
     5641 gtagctcaat taattaggtc agaaagttac ttttttaaaa ggacaccaga gtataccctg
     5701 ttgggctctt ggtgttctat tgaatgtaaa tgagtgtcat ttttatattt aattggcaca
     5761 gttttttcta attatttgtc atattcatag tcaactatag ctacaaaagc aactaatttt
     5821 ggagatagat tgagatattt aaaaatctat catgaaaaat tagttgcttt tgtcacttag
     5881 cgctgaaaat ttatgccatt tatctagaaa aatgacaaca gagttgggca acagaattta
     5941 aaaatgatcc ggatttcaga attttaaagg agttttatta tggcgaaaat tgggtatgct
     6001 cgagtaagca gcttagatca aaatttagac cgacaattag aattattatc agagtgtgat
     6061 aaaattttta gtgataaagc aagtgggaaa gataccaatc gagaaggatt tcaagaaatg
     6121 atgaaattca tacgtgaagg tgatattgtc gttgtgacag aattaaaacg acttggacga
     6181 aataataaag agctaacaga aacgatgaat ttaattcagc tgaagaaagc tacactggaa
     6241 gttttgaatt tgccagcgtt aacaggtatc actgatgata atttacgtcg attacttaat
     6301 aacttaatta tcgaactata caagtaccag gccgaagaag aacgtcggta tattcgcgaa
     6361 acgcaacaac aaggaattgc actcgctaaa gcaaaaggga aatacaaggg aggtacgcca
     6421 aaatatcgtg agaatgatcc gcgacttcaa ttagcattta atttgttttt gactggatct
     6481 acagataaag aagttgaaga gctaacaggg attaatcgta gaacgtttag aagatatcga
     6541 atgaaacatg gtattactaa tgaagttagg gagcaaaaaa ataaatcttt cagttgaggc
     6601 taatataatt ttgcaagaaa gggattttta tgaaagaaat tagattttca aatcaaaata
     6661 tgcagaatta ctataatgaa attatgaata tggaaactag tgaattg
//
LOCUS       DACDLI010000104         6598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDLI010000104 DACDLI010000000
VERSION     DACDLI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.104"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..203
                     /locus_tag="IV353_002486"
     CDS             <1..203
                     /locus_tag="IV353_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9010676.1"
                     /translation="KKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTSL
                     LIISLVIYFLKIKNRIIDDYNS"
     gene            216..1223
                     /locus_tag="IV353_002487"
     CDS             216..1223
                     /locus_tag="IV353_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9010677.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1235..2239
                     /gene="neuB"
                     /locus_tag="IV353_002488"
     CDS             1235..2239
                     /gene="neuB"
                     /locus_tag="IV353_002488"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9010678.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2236..3384
                     /gene="neuC"
                     /locus_tag="IV353_002489"
     CDS             2236..3384
                     /gene="neuC"
                     /locus_tag="IV353_002489"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9010679.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3357..4034
                     /locus_tag="IV353_002490"
     CDS             3357..4034
                     /locus_tag="IV353_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9010680.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            4024..5166
                     /locus_tag="IV353_002491"
     CDS             4024..5166
                     /locus_tag="IV353_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9010681.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5179..6156
                     /locus_tag="IV353_002492"
     CDS             5179..6156
                     /locus_tag="IV353_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9010682.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6156..>6598
                     /locus_tag="IV353_002493"
     CDS             6156..>6598
                     /locus_tag="IV353_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9010683.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDW"
BASE COUNT         2461 a          825 c         1244 g         2068 t
ORIGIN      
        1 ctaaaaaaat tacgaataaa caattatttg acacaaaaaa aatatgtatc ataataattt
       61 ttgtatcaat tttttctttg atacttcata ttttaatcaa tcaaatgttg attcgttacg
      121 ttttgacatc attattgata attagtttag ttatatattt tttaaagatt aagaatcgaa
      181 ttatagatga ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag
      241 taactggcgg aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac
      301 caaaagctat cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc
      361 tctcaaatca cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg
      421 attatgcatg tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag
      481 caagtgaata taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa
      541 tagatgcagc gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag
      601 tgagtcctac taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg
      661 ccaatcagta taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca
      721 tggggtcaag tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata
      781 ttactttgac agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat
      841 tagttctgtc tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg
      901 caatcaatat tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg
      961 aaaaatatag aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag
     1021 agctaatgac tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc
     1081 caccacacta tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg
     1141 catactcttc agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag
     1201 agaataatgt cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc
     1261 agaaattggt gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc
     1321 taaagaatgt ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc
     1381 caaaaaggct ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt
     1441 tgaaatgcta aaaaaattag aattatctca atctgatttt attagaataa aagattattg
     1501 tatacaaaat aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct
     1561 aaaacaaatt ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt
     1621 tcttagaaaa atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat
     1681 tcatgaggtc agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg
     1741 tacttcaaat tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa
     1801 agaaatgttc gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat
     1861 ttgtgctgtt gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact
     1921 acctggtcca gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca
     1981 tattagaaac actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact
     2041 tagtacaaaa gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga
     2101 tattcttaca gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt
     2161 tttatatcaa tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct
     2221 tgaggaagat gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga
     2281 atatataaac ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt
     2341 tctggaatgc atttatccca taaatatgga tttactaaag aattgatctt agctgacgga
     2401 tttagaatta gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct
     2461 cggtctgtgg cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat
     2521 tttgtattat tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat
     2581 cttggaatca aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca
     2641 ataagacatg ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa
     2701 gagcgtttaa taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga
     2761 atagatacta ttttaaataa tagattacct agttttagag atataaaaga aaaatatcat
     2821 ctaaaatttg aaaagaaaaa atacgttttg caagtatttc atccagttgt aaccgaacta
     2881 gaaaatatag aaaaacaaat tttagctttg atcaattcaa tggaacaaca atctcaacca
     2941 attatttgca ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag
     3001 aaatatttaa aaacggatat tatttatatt acgaatttag agccggagga atacctgact
     3061 atattaaaag agtgcaaatt tatgatcgga aattcttctt ctgggataat tgaagctgca
     3121 tcattcaaaa taccagtaat aaatttagga accagacaaa atggacgaga acaatctaaa
     3181 aatattataa atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa
     3241 gatcctcagt ttataaaaag cttgaaaaat gttaagaaca tctacggtga cggcaacagt
     3301 tctagaagaa taacaaaaac attgaatcaa atattgaatg atggggaaga tattaagtgg
     3361 attcaaaaac aaatagctta ttaaaatata ctagtgatcc atacattatt attggggctg
     3421 gcggacacgc aaaagtcatt attgaaacct taagattaga aaataagtta atatatggat
     3481 tttttgatga taagcaaaat aaaatgcaag atctgaaata tcttgggaaa ataaacgaat
     3541 tgaatagctg ggcggatagc aaaaaaaaaa tctataaatt tattgtagct attggtgact
     3601 gtgtagtaag aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag
     3661 ctgttcatcc aagtgctata gtggcaaaag aaatttcaat tggttatgga actgttattt
     3721 ttgcgaatgc tgtaattaac ccagatgctg taattggaga gcatgctata ataaacacag
     3781 gtagcattgt tgagcatgat tgtagaatta ataactatgt tcatttatct ccaggtgtat
     3841 gcttagcggg tggagtacat gtaggtgtag gaactcaaat aggaattggt tctcaatgta
     3901 tccagatgaa gaaaattgga tcttggtgta tgataggcgc agggagcact attgttaagg
     3961 atattccctc acattcctta gcttatggca atccagctaa aattaagaaa gaaggaatta
     4021 attttgaatt ctagaatttt attatcaagt cctcatatgg gaggaacaga agaaaaatat
     4081 gttgctaagg cttttaaaga taattggata gcgccattag gtgaaaatgt ggataaattt
     4141 gaagaaagta ttacaaaata tactggttct aagggcggag cttctgcgtt aagttcaggt
     4201 acagctgcca tacatcttgc cttaagactg cttaatgttg gaaaaaacga tattgtcttt
     4261 gctccttcat ttacttttat agctacagta aatcctattt tatatcaagg ggctgtacca
     4321 gtgttcattg actcagaacc taatacatgg aatatgagcc ctgttgcact agagagcgca
     4381 ttagaagaag ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt gaatttatat
     4441 ggtcaaatgg ctgattatga acgaatttta gctattgcaa accaatttaa tgttccagtg
     4501 atagaagatg cagcggaatc gttaggttct acttataagg gaatttcgtc aggcttattt
     4561 gggacaattg ggatctattc atttaacggt aataagatca tcacaacctc aggtggagga
     4621 gctctagtgt ctagtgataa aaaatatgtt gagcattcaa gatttttagc aacacaagca
     4681 agagatcaag caccgtatta tcaacatagc gaaataggtt ataattaccg tctaagcaat
     4741 atttctgccg gtatcggacg tggacaaatg gaagtattga atgatagagt agctaaacga
     4801 agagaaattt tcaatcgata taaggaagct ttttctggaa ttgatagaat tgaaatgatg
     4861 ccagaagtgg atggcatggt gccaaatcgt tggttgtcta ctttaacgat caatgattat
     4921 aattctattg atcaagtagt taacgtaata aataaactaa atgaaaaaaa cattgaaaca
     4981 agagctttgt ggaaaccact acatttgcaa ccagtatttc aaggggtaaa aatgtattac
     5041 catgataata atcttagtgt gtcagaaaag ttatttaata gcggcttatg cttaccttct
     5101 ggaagtaata tgacagatag tgaacaagaa tttgttattg atgagtttaa aaaaatattc
     5161 aagtaggtgt gatattttat gaacatagga attattggtt taggttccat ggggaaaaga
     5221 agacttagac tcattcaaga acactttaat gatattttac tatgtggtgt tgatactaat
     5281 gaggcgagac gaaaggaagt tgaaaaggaa tttggaatac caacttataa tagtctaaca
     5341 gaggcaaaaa atttgtttga aataaattca gtttttgttt gtacatcccc tataacccat
     5401 gaatttatta taaaagaggc tttaaattta gattgtaata tctttacgga aatcaattta
     5461 ttaaatgaat attatgatga aataatcgaa gtagctaaaa agaaagatag attactttat
     5521 ctatcttcta catttttgaa gagaaaagaa attcaatata ttaagtctaa agtgacgcat
     5581 aataaaaaaa tttcttatca ataccatgta ggacagtatt tacccgactg gcatccttgg
     5641 gaagattatc gcaatttttt tgtttcaaac aaaaaaacta atgggtgtag agaaatattc
     5701 gctattgaat taccttggat tatagataca tttgggaaag ttaaatcgtt tactgtaatg
     5761 acccagaaat tatccaatct tgatattgat tattcggaca gtttcactct tattattgag
     5821 catgaaactg gtatagttgg aactttaagt gtagatgttg tttcaagagt agctaaaaga
     5881 aatttaacta taattggtga aaagatttta atagaatggg aaggtaaccc ggattctcta
     5941 ttttcatgga atgaagaaac ttctagtatg acagatattc aattgtacga tgaggttatg
     6001 catgataata attattcaca tactattatt gaagatgcat atttagagga gattaaagaa
     6061 tttttcacgc ttttggaaaa gaaaatggaa ccccaatata cttttgaaaa agataagtat
     6121 attattaact taattaacga cattgaggta aattaatgat gaaagtaatt ttttttgggt
     6181 tagggtcaat tggaaagaga catctaaaaa atctaataag ctatggaaaa aagaattcta
     6241 ttaattttga tataacagct tatagtagca gtggaaaaaa agagtttgaa gaaataacgt
     6301 atattcaaaa tagagacgat ttggatacac attatgatat agcttttatt acaaatccga
     6361 cttttaagca tctggaaaca ctaaatgtaa ttgttgaaaa agttgattac atatttttag
     6421 aaaaacctgc tttttctgag agtattgatc ttactcaata taattatgat tacaaaaata
     6481 tttatgttgc tgctccactg aggtataaaa atatcatgga agttgttcgt aatagtattc
     6541 aaagtaaaaa agtatattct gtaagagcta tttgttctac ttatcttcct gattggag
//
LOCUS       DACDLI010000105         6432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDLI010000105 DACDLI010000000
VERSION     DACDLI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.105"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV353_002494"
     CDS             complement(99..1229)
                     /locus_tag="IV353_002494"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9010684.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IV353_002495"
     CDS             complement(1878..3080)
                     /locus_tag="IV353_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9010685.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IV353_002496"
     CDS             complement(3140..3343)
                     /locus_tag="IV353_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9010686.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IV353_002497"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IV353_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IV353_002498"
     CDS             4880..5692
                     /locus_tag="IV353_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9010687.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1930 a         1313 c         1008 g         2181 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcataga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatattttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt ca
//
LOCUS       DACDLI010000106         6143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDLI010000106 DACDLI010000000
VERSION     DACDLI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.106"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(149..898)
                     /locus_tag="IV353_002499"
     CDS             complement(149..898)
                     /locus_tag="IV353_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9010688.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1084..1788
                     /locus_tag="IV353_002500"
     CDS             1084..1788
                     /locus_tag="IV353_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9010689.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            1828..2385
                     /locus_tag="IV353_002501"
     CDS             1828..2385
                     /locus_tag="IV353_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9010690.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2393..3532
                     /locus_tag="IV353_002502"
     CDS             2393..3532
                     /locus_tag="IV353_002502"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9010691.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            3544..3975
                     /locus_tag="IV353_002503"
     CDS             3544..3975
                     /locus_tag="IV353_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9010692.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4021..5433
                     /locus_tag="IV353_002504"
     CDS             4021..5433
                     /locus_tag="IV353_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAP9010693.1"
                     /translation="MRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALFIPTGWFPNESL
                     NELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIPMFMGAMIMGPL
                     GGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPIIQSANQFVTSA
                     IEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGRSIYFMVASNPG
                     PGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLTILSMIAGGMTG
                     IITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSFFVTSVILKADK
                     STDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFACDAGMGSSAMG
                     ATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAHPNMKIVTLKNY
                     LQDPVINGLAEEIIGLKTED"
     gene            5719..>6143
                     /locus_tag="IV353_002505"
     CDS             5719..>6143
                     /locus_tag="IV353_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9010694.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSV"
BASE COUNT         2051 a          897 c         1272 g         1923 t
ORIGIN      
        1 attgctatag tatgttatac gtggtgctag gtaagtgaga ataaacaaaa aaggaaacac
       61 gcaataaagt ttatttcaaa actttattgc gtgtttcttt tttagatttt ttatatctgt
      121 tgctttagta gttaccaata gttttcgctt aatcaatcaa aacgattttt tgtttattgt
      181 attctttacg aaccttttca ggaatttctt ctgaaataag ataggtaagg tcttcaattg
      241 gaccgacgat atgattactg atggtgccga atttatcatt agttatcatc ccaagtactt
      301 cagtagaaat acgtatcatc tcacgtttaa tgtcggattc ttgcatagtt ggcacgctcg
      361 ttccaacatc tagatctatg ttataaatac ccataatata aaggtcggct cgaatgtttt
      421 gtagttgctt ataagcttgt accccaatat tgatcataga ttgtttataa aaagtaccgc
      481 caatattgat aatttccaaa ttaggatgct gtgctaatgc tatggcaatc ggcgggctat
      541 tggtaatgat tgttgcacga aaatcttgtg ggatttcttt tgctaaagcc aagttagttg
      601 tacttccatc aatgatgatg acagattctt cttttaaaaa agggagggct ttatgagcta
      661 atgcagtttt ttgttcgcta gctacagttt ctcggtagtt aaaattggta attggcggac
      721 caacacgtaa agcgccgctg tgtactcttt ttaagagacc ttggctatcc aaatctttca
      781 aatctcgtcg aatcgtgtct tcggatacat tgaaacgttg acttaaatca ctagcgataa
      841 ctttttgttc tgcgtttaat aaatctagaa ttttttgatg acgttctaat ttcaacatgt
      901 tgcgtgctcc ttcttattta ttcttgttta tcatgtcatt tatgcgtttt tatgtcaata
      961 atttccgttt ttaatacact aaaaaggcat aaacaagcaa aaacaaaata tttttatgca
     1021 gttttatgtt gactagtatt tgttaagcgg ttacaatgag ggggtaatta aaggagggaa
     1081 tgtatggaga aaaaaattat tttgtcgcct tcacttatgt gtggggatct agcaaatttg
     1141 gaaggaagca tccaagaact agaaagtatt gggatggatt ccttgcatat tgacgtgatt
     1201 gacggttctt ttagtcccag tatgccttta gggatcgaaa caatcaaaag aattagagaa
     1261 atcacttcaa tgaattttga tgtacacatc atggctaagg ataacgataa cgaatttttt
     1321 attggagaaa tgttgaaaat tggcgtagag agcattactt ttcattatga aacaagcctc
     1381 catattgatc gatatatcca gctcatcaag aattctggaa caaaagtagg gatagctttg
     1441 aatccagcaa ctccgttaaa tgtattacaa tatattttac ccgagattga taggatttgt
     1501 ttgatgctga ttaatccagg ttttgcaaca aacaagtcag aaaaacaagt ttcttacgcg
     1561 ctaaaaaaaa ttacagatct ttgccagtta gtaaatgaga aagagttagc agtagatatc
     1621 caagtggatg gacgggtatc gttagaaacg atcccgggat tattagatgc aggcgcaaca
     1681 aatctggtat taggaagtac aagtttgttt cttaaaggaa atacattagc agaaaatcaa
     1741 aaactacttt atcaagcgat gaagcaatca gttaggaaaa atcaatagat gtagtggtcc
     1801 tgaataatag aaatggaggg gaaatagttg agttataagg cggcgaaaca aaatattgta
     1861 agagaaatta ctgaaacatt agaccgtatt tcttcaaaag atgtggaaca attgataaag
     1921 aaaattgaaa aagcggaacg agtttttttc gtgggggttg gcagagtact gttagcgcta
     1981 gaaggaatgg caaaacgctt agggcatcta ggaattcaga cagtagtcgt aggacaaatc
     2041 actgaaccgg caattacaga taaagattta ttgattgttg gttctggtag tggggaaagt
     2101 gctttcccac taactattgc aaaaaaagca aaggaatttg ggtcccaagt tgcccatata
     2161 ggatcaaacc cagacagcac aatgaaagaa tattcagatc tttttattcg tatacccgta
     2221 caaacaaaat tagcgttacc agaggaaatt tcttctatac agccaatgac tagcctgttt
     2281 gaacaaagtg ttcttttgtt aggagatagt attgctttag tgatgataga agaaaaagca
     2341 attgatatgt caaagttgtg gcaataccat gctaacttag agtaggtgaa aaatgaaagc
     2401 ggtacatttt ggagcaggga acatcggcag agggcttatt ggacaaattc tacatcagaa
     2461 tggattcgat ctatgtttta ttgatacgaa tcctgaactg atccatcaat taaatcaaga
     2521 taagggttat actatcgaaa ttattgatga aatgaacagg agtgatttca ttgatcatgt
     2581 tgtggcactg aatagtttat ccgagctaga agaggtgatt acagcaacaa aaaatgcaga
     2641 tatcttaaca acatctgttg gcgcaataaa tttagtaaaa attgcaccga tcattgcgca
     2701 agccttaagt aagcgatttg aacaaaagca tagcatcaat atcgtagcta atgaaaatgt
     2761 gataaatgca agtgatgtgt tgaaaaaaga aatttattct ttgctgtcag aaaaagaaca
     2821 agagaatttt gatgaatatg catattttgt gaatacggca atcgatcgac aatctttagg
     2881 aaaagttctt ggaggaaaaa tgatctctgt tgtcgaacct tattatgaat ggataatgga
     2941 tcgacagcag ttagatccaa ctacatcttt tgatatgaaa aatgtaaatt tggttgatga
     3001 tatggagcct tacattgagc gaaagcttta tatcgttaat gctgctcatg cggcagtagc
     3061 ttatttaggc gcactgaaag gatataagac gattcaagaa gcaatccaaa atatggagat
     3121 tttcaatcta gtcaagcaat ttttaatgga gaatatagag tactttgtac aaaaatatca
     3181 tttttctaga gaagaattaa catcttttgt ggaaaaaaca ttaaaccgtc atgggaatca
     3241 gaaactctcc gatgaaatta ctcgtgtagg aagttcccct attcgaaaat taggccctaa
     3301 tgatcgttta attgctccca tagtaaaact agattcatat catttacctt acgatgcagg
     3361 aataaaagta attgctgcgg gatatcatta tcggaatctt gctgatccag aagctgaaca
     3421 actacgtcga atgattgtaa atgatgggtt gaaagcaacg attaaaaaga tttctcgttt
     3481 ggaaggtaag ctgctgaatg aagttgtcgc tgcttatgag tcccagattt agaggaggag
     3541 aatatgctaa aagaacaatt gtcaggaaat acaatgttta tggatgcgat cgattcatgg
     3601 caagaggcaa tccgagtaag tgcgcgtcct ttgataggaa agggaataat agaggaaagc
     3661 tatgtacaag cgatgataca aaatgtgttg gataacggaa attatattat attactgcca
     3721 ctagtagcaa tgcctcatgc gcgtccagaa tttgggagca aaggggtcgg gatgagcttt
     3781 ttatgtttag ataagccagt catgttccca agtgaagaac cagtaaaatt cttctttacc
     3841 ttttcttctg attcgccaaa gggacattta gacttaatgg ccaatcttgg agaattgctg
     3901 agtgatgaaa aaatgtatca aaaattattc gatgtgaaga cagaaaaagc attgttagat
     3961 ttgataaaga agtaaaggag agaagaaaat ggaggtagca aacaaaaaaa acgagtctcc
     4021 atgcgagcta aagttcaagc gattggcggt ttcttaacaa atatggtatt gccaaatata
     4081 ggcgcattta ttgcatgggg aattgttact gcattattca ttccaactgg gtggttccca
     4141 aatgaatctc tgaatgaact cgtaagtccg acagtcagct atctattgcc gttactactt
     4201 gcctatactg gtggtcggat ggttggtggt acacgtggag ctgttttagg tacaatcgga
     4261 acaataggtt tgattattgg tgctgacata ccaatgttca tgggagcaat gattatgggg
     4321 ccacttggtg ggtggatgat gaaaaaaaca gatgattttt tagatgggaa agttcctgct
     4381 ggttttgaaa tggtcgtcaa taatttttct ataggaatta ttggtttcat actgatgtgc
     4441 ctttcttatc tggtagttgg accaataatc caatcagcta atcaatttgt aacgagtgca
     4501 attgaagcat tagtttcaac tggtttttta ccgatactgt ccttaatcaa cgaaccggca
     4561 aaagtcttat ttttaaataa tgttattgac caaggtgttt attatccatt aggtatgcaa
     4621 caaacattag aggcaggcag atcaatttat ttcatggtag catcaaatcc tggccctggc
     4681 ttaggactat tgttagctta ttctttattt ggtaaaaagg cagcaaaaca gacagcacca
     4741 ggtgcaatca tcatccattt tttcggcgga atacatgaga tttattttcc atatgtatta
     4801 atgaaaccac tcactatatt atctatgatt gctggtggaa tgacaggaat catcactttt
     4861 gaattatttg atgcgggcct ggtagcaggt ccaagtccag gttctatttt ttcttattta
     4921 gctctaacgc ctcggggaaa ttttttgggt gtcatggcgg gagtggtcac agcaacactg
     4981 gtttcctttt ttgtaacatc agtaatttta aaagcagata aatcaacaga tgaagacaga
     5041 aattttgaac tatcagttca aaaatcgaaa acgatgaaat cagaaggaag aaaattatta
     5101 aaagaagaaa atgctagaga aatatgtacc gagaaaaaag agatgaataa aatttccttt
     5161 gcttgtgatg caggaatggg aagcagcgca atgggcgcaa caatattcaa gaaaaaattg
     5221 caaaaagcag gtattactga tgttgagata aaaaattatc gaattgaaga ggtaccagaa
     5281 gatagtgatt tggtgatcgt acatcaagat ttggaggaac ggactagaaa agcacatcct
     5341 aacatgaaaa ttgtgacatt aaagaattat ttgcaagatc cagtaatcaa tggattagca
     5401 gaagaaatca ttggattaaa aacagaagac taatttatgg gaatttttga ttaaccttta
     5461 ttaaaattat tttgcctaat ataaaaaggt tgtgacatgg cttgtgtcgc aacctttttt
     5521 tgtcgcacaa ataattaatc ggatatttta tgttcatatt ttttgtgatc aacgagaact
     5581 gatggggcaa agatacattt tagatacttt taactgttaa tttacaaaac aaggtgaaac
     5641 gaatagaaaa atgaaaaaat cgatacggaa gaatatgttt gattaaaaca tgcatgaaga
     5701 gaaggaggtt gatgatacat gaaaaaaata tattcaaagg aaatcgattg ttttagaggc
     5761 attgctgttt tagcagtgat attttatcat ttgccaaata gtttgctgcc aggcggattg
     5821 caaggagtaa cgatcttttt tgtggtttct ggttttttaa tggctaaaaa tacgataaga
     5881 gatgaaaaaa acagacaatt cagtttttta catttttact ataagcgtat caaacgtatt
     5941 tatccatttt tactgttttc tttggtcgta ttcattttca tactagggat tagcaacatt
     6001 cgattgatag gaaacattcg tagtgagcta cccagtttga tttttggcta taacaattgg
     6061 tggcagctga atcaaggagt atcttatttt gactcttact ttaatgcttc tttatttaaa
     6121 cattattggt cgctttcagt tga
//
LOCUS       DACDLI010000107         6048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDLI010000107 DACDLI010000000
VERSION     DACDLI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.107"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..722
                     /locus_tag="IV353_002506"
     CDS             <1..722
                     /locus_tag="IV353_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP9010695.1"
                     /translation="EMTDLWGIGSRMKKRLNQIGIMSIKDLANWNPYTIKSHLGVIGL
                     QLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIR
                     QHNCKTGCVHLYIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRH
                     VGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSMLEGAR
                     SITRSTLVGGHAGGAGGLKND"
     gene            715..1065
                     /locus_tag="IV353_002507"
     CDS             715..1065
                     /locus_tag="IV353_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010696.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(2136..2411)
                     /locus_tag="IV353_002508"
     CDS             complement(2136..2411)
                     /locus_tag="IV353_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9010697.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(2383..3336)
                     /locus_tag="IV353_002509"
     CDS             complement(2383..3336)
                     /locus_tag="IV353_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9010698.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            3948..5441
                     /locus_tag="IV353_002510"
     CDS             3948..5441
                     /locus_tag="IV353_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9010699.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            5576..5875
                     /locus_tag="IV353_002511"
     CDS             5576..5875
                     /locus_tag="IV353_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9010700.1"
                     /translation="MLIKNYVYSHPVNVFIIGRLGLSVELFCELYGFKQGTLSSWVAR
                     EKTVATLPIEFLHALSLACGETMDQVYDCLCVLEQEYIEFKKDNKSKKRKKYIQK"
BASE COUNT         2077 a          965 c         1107 g         1899 t
ORIGIN      
        1 ctgaaatgac agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg
       61 gtatcatgag tattaaagac ttagctaatt ggaatccata cactattaag agccatttag
      121 gtgtgattgg cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga
      181 tcccaccaga accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt
      241 ataccaggag aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc
      301 gtatcaggca acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct
      361 tagaaacgag accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg
      421 aattgcagaa ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc
      481 acgttggaat aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta
      541 gtgatccaca aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc
      601 aaaaatatgg ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta
      661 ttactagaag tacattggtt ggtggacatg caggcggcgc aggagggctt aaaaatgatt
      721 gattataatg atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa
      781 aaaattgatg ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg
      841 actgaagagg ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt
      901 caaaaagaag aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta
      961 ggtagtgatg agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt
     1021 catatcgaat tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat
     1081 atgagctaaa gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc
     1141 gtttgataaa tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat
     1201 accataaata cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg
     1261 ttttacattt tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa
     1321 actagttaca accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata
     1381 gcactagaaa gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta
     1441 ttcagctaaa tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg
     1501 aataacgcaa gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa
     1561 cattgcgtag ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt
     1621 atgatgctaa gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct
     1681 cctttttagg ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt
     1741 gattgagtct agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa
     1801 cataaatgaa acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg
     1861 atttattact tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt
     1921 gttaaaacca ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa
     1981 atatatttag tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa
     2041 acaatacaca tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta
     2101 aatatattta ctatatctag tatacttact atattttaat cttttgatcg tctattatat
     2161 ataatccgtt cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt
     2221 tcatacatag gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga
     2281 tctacttgaa ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta
     2341 atatcatctt taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt
     2401 aataatgcca tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc
     2461 agcatattta ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat
     2521 tctttcttct aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata
     2581 atcatttaat tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat
     2641 gcttttaaaa atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg
     2701 agaggaacgt cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc
     2761 ttcaaaattc gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat
     2821 tgcgtaccgt tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg
     2881 aaccacatta tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa
     2941 ttcttctaaa ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga
     3001 caagtattca ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact
     3061 tacttgagta atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc
     3121 aaagctagta atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata
     3181 atcaattagc aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt
     3241 gtctgtagtc ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt
     3301 atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta
     3361 aatatattta gtatacttca tatacttaat atattcgata tatctaatat actccaaatt
     3421 gataagaatt cagactttat aaggaatata gtggatatac taaatatatt tgctatattc
     3481 agtatatcag atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta
     3541 gagtgaaaag aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt
     3601 agcaaaagca gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa
     3661 ttgattttag cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg
     3721 tctcaagaat atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt
     3781 ccgattgcct ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag
     3841 tgtacttaaa ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta
     3901 aagctaaact gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa
     3961 tttattcagc tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga
     4021 aacctgcagg tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc
     4081 aattaatgaa tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg
     4141 aaaagaaact aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt
     4201 atcgaacagt ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag
     4261 ttgatatcga ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag
     4321 tgggctacat gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac
     4381 taaagacacc aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa
     4441 aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat
     4501 gtaaccactt cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga
     4561 actatcagta ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa
     4621 aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc
     4681 aagtagatga accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag
     4741 gacgtcttgg tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt
     4801 atgcattgga tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc
     4861 tatctgaagc agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag
     4921 caagcagaga attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata
     4981 atgatctgtt cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa
     5041 aatatagtca taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc
     5101 ttgctagtga gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc
     5161 ccaagcgaac acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca
     5221 aggtaacacg aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga
     5281 gggttattca actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat
     5341 ttggactcaa cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc
     5401 ctcttaaaga agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt
     5461 gccttataca atatatctat gatacttctg taggtgttgc tgcctttgct tatctacgtg
     5521 taggtggtat acttatcact gcgtatgcag ttttgtctag cttggaggac ttactatgtt
     5581 gataaaaaat tatgtctatt cacatccagt taatgtgttt atcattggtc gtttgggatt
     5641 atctgtagaa ttgttttgtg aactttatgg ttttaagcag gggacgttgt cttcttgggt
     5701 tgctagggaa aaaacagttg ctactttgcc gattgagttt cttcatgcgt tgtcattagc
     5761 ttgtggggag actatggatc aggtatatga ttgtttgtgt gtgttggagc aagaatatat
     5821 tgagtttaaa aaggataata aatcaaagaa aaggaaaaag tatattcaga aatagttttt
     5881 atttacttaa tgttgaggaa gttattcctg ctaagtttga gaaaaaataa aaagcagcgt
     5941 ttgaagactt actaacagtt cgaacggaaa aatggctaga gtagtttata taaaatgctc
     6001 atgttcctaa aaaaagacgg tcaatattaa attaaaattt ttttataa
//
LOCUS       DACDLI010000108         5933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDLI010000108 DACDLI010000000
VERSION     DACDLI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.108"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1059..2027
                     /locus_tag="IV353_002512"
     CDS             1059..2027
                     /locus_tag="IV353_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9010701.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            2044..2568
                     /locus_tag="IV353_002513"
     CDS             2044..2568
                     /locus_tag="IV353_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9010702.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2944..3225
                     /locus_tag="IV353_002514"
     CDS             2944..3225
                     /locus_tag="IV353_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010703.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3227..3643
                     /locus_tag="IV353_002515"
     CDS             3227..3643
                     /locus_tag="IV353_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010704.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4274..5428
                     /locus_tag="IV353_002516"
     CDS             4274..5428
                     /locus_tag="IV353_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9010705.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            5422..5844
                     /locus_tag="IV353_002517"
     CDS             5422..5844
                     /locus_tag="IV353_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010706.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT         2259 a         1016 c          954 g         1704 t
ORIGIN      
        1 ttttgagggg gattcaaggg ggagcttgcg cccctttgac aagcggttat ttgcaccaaa
       61 tacgtaggta tttggctagc aaatatagag cttgccaaac actttagtga gttttgtaga
      121 aagtaccttt aaaggtactt tcacattacg aacgatacgt ccgtaggacg tactgaaagg
      181 gtgggattta tgagcgaact aatgcaaaat ttggctagca aaaaaagtta aaaaatatat
      241 gctaataatc gagcgaaatg aacacttttt caagtgagcg aatccgccat gacaaagctg
      301 aagcagttta tttcagcttt gtcatggcgt gtgcctagac ttttaaaaaa gtcggcacac
      361 gagcaaatat ctttatcaag ctctaatttg cgttttaaga cgtttgaaac gtctaagtat
      421 ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa ttttagaggg cattcagaca
      481 actttagggt tgttagggct tgccctgacc gcctgtaagg cgctttaaga acaagataag
      541 ggatttatac taagactgtc ttaaaacgtc tcaaacgaag gctcatagct tctcaaagag
      601 ttccttatga acatttttag caaaggagcc ggaagcgact gcgcagtata atgtgagagc
      661 agagcgatca caccttttta agcgactgta gggagtaaga ttcccagttg agaaaaatgc
      721 gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg ggaaattttg tccccctctt
      781 ttataatctc tttttaaacc tctttttaaa cctctttacc gtcggctata tccttactct
      841 cccaagggaa tgacgctttt atctacatcg ttttgtctgt ttatctacat cgttttgtct
      901 gtttatctac atcgttttgt ctgtttatct acatcgtttt gtctgtttgt acattgaatt
      961 agtacatcga cgatgttaat ataagatgta gataaacagg cattttgtat ttcataacgt
     1021 gtatctcttg tgcgagtaag aaaggagcta ttttaaatat gtctaatgaa atcgtaaagt
     1081 atcatcatga attaaataca attccgttgc gaaaatttac accagtcgaa atgaatttgt
     1141 tcttttctat cgtatcaaga atgcgtgatg taggtgataa aaaagtacaa tttacattcg
     1201 agcaattaaa agatttaagc aattataaag caactgcaaa tgttcgattc atagatgatt
     1261 tagaaacaac gtatgacaaa ttaatggatt tacgttttgg tagaagaagt gctgacggtt
     1321 tgcaacgtga acgttttgtt ttatttaatc aatttaaaat cgacggaaaa tctgatattc
     1381 cttttgctga aatacaagtt catgaaaaag ccttaccctt acttaataat cttgaagaat
     1441 gggtgcgcta ttctctccaa caattcaatg aattagaaag cagttattca aaaacaatgt
     1501 ttcgattact aaaacaatac agaaccaaag gtttcgctta tttttcaaaa gaagactttc
     1561 atgaattact tgatattcct aaaagttaca accaaggtac gattgatcaa cgtgtgttca
     1621 atccaatacg acaagaacta accgcaatct ttaaaggttt aacaatcaaa aagaaatacg
     1681 gaaaaggacg gggaaagcct gtcattggct atcaatttac atttaaacct gagataaaaa
     1741 aatcagacga ctttaacaaa catgccccaa caaaagaaga acaaaacaaa gcattagaac
     1801 tagctaacca acaagttcaa tctgaaaaag cacctacttc attacaagaa aaaatcaaag
     1861 aacaaaagcg aaaattggaa cttttacaac agttagaaaa gttagaaaga gaacaaaata
     1921 cccaaaataa agaaaataat gcacaaaaat cagagataaa ttccgatatt ccttcagaac
     1981 taaaaaaaga cttattagac aatcttcaaa acttattcaa agattgaggg gtttttagat
     2041 tttatgacag aaaaaacaat caaagaactt gctgaagaat taggagttag taaaacagca
     2101 attaacaaaa aagtttctga cgaaaatcga aaactttggt tttcgaaaat cggaaacaag
     2161 tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa tgttttttaa cgaaaacgaa
     2221 aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg aaaaccacaa aaacgccaac
     2281 caagttcccg aattagaagt cctttctctc ctagaacaac agttaaatca aaaagatatg
     2341 caactacaag aaaaagacga gcaaataaaa caaatgcaaa aattactcga ccaccaacaa
     2401 caactcaccc tgcaagccaa ccaacaaatc gagaaactgc aggaacaact acagttgact
     2461 tatacggaag aatcttcgga aaataaatca acgactcttt ctgaagaaac agaatcgata
     2521 gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct ggcaataaaa ccagcaattt
     2581 aaattaataa aaattgctgg ttttattctt ctttttgctt ttgcgagcag agcaacatgg
     2641 acccatgttg cgatattttg gtggcgcccc aatccccgtt ttttcgttga atcttactcg
     2701 gactgcactc gttttacagc tgatttttct gaattttgaa agagcataag gaaacgtggt
     2761 atcactttct tacgaaaggt gctagatagg ctgtgcctat cacgtttctc agaaaaaaat
     2821 ttgctatcac ttttttgcca ttgtggctag gctatgtata gtgttttggc gaaaaatagc
     2881 tatacaaata ctatatttgt atggtataca taaattatac gctgatacga aaggaaaatt
     2941 tccatggaca ttttagttcg aaatatcaat cccaaaatta ttgcaagaat agataaacgt
     3001 tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc gaaataaata tctaaaagtt
     3061 ctaatcgaaa gtgattttga ccgtcaactt atgaattaca aaaaagatca attcgattta
     3121 ttactcgaaa aatttattgc cattcaaaca gaaaatacga acatactgaa tgactatgtt
     3181 cacacaaaca accaactcat tgaaacactt atcggaggaa tataacatga gaaaaacttc
     3241 tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac gttttagatt ccatcatcga
     3301 aaaaaatcca gacgtcaaca attattcaga agccattcgt tttttatgta tgagttatga
     3361 acctgatacc aatacaacca caaccagtaa ccaagcaaaa ctcaatgcca tgggaaaaga
     3421 aatttctatc atgacagaaa tcatggcaga atttgctgat ttatatctaa aagatactgg
     3481 tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg aaagaaaaaa ttgaaaatca
     3541 actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa aaagaaacca atcaaacctt
     3601 tacttctgga aattttgacc caacaaactt tctaaaaaga taaaaaaaag aaaccgatcc
     3661 ctaaaaaaat tcctcgattt ctttttaggg atcagtcttc cagcaagatt tgcaagttgg
     3721 taccaacttg cgaaaaaaaa gaaaaatccg cttatgcaac ttcttctttt tgtattttga
     3781 ggcgctaccc caatccccaa gtatcggtta tttaatagcg cggaaatcca aagaatagga
     3841 cgtaccctga aacaaaaaaa gactgctctc acgagcagcc caaaatgaga ttatcctatt
     3901 catcaacata aaaatattac aaagtgaaga actagagaaa gacaaaaaaa gcatatcaaa
     3961 tcctctcaaa aataacaagt ctttaaaaaa tgacagataa aaatgcacaa agaactaaaa
     4021 aagagaaatc gcgtaaaatc aagcattaga agctagtaac tagctagacc aattaaaaat
     4081 cacaaagtga gcgtctctat ccattggtag aggggctact tggcatagtg tacattatgt
     4141 taactagctc gataaataac gattatgttc ccaaaacaat attttatgaa ctataacaaa
     4201 aatttgttat cgcatattgt atattttaat tatataatta taggtgtata ccacttaaat
     4261 ttaacacaaa gacgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
     4321 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
     4381 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
     4441 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
     4501 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
     4561 tttctttcca gtattttcag acgctgataa tacgattaaa tatatttata ccgtcaaaaa
     4621 aaatacaaat ggagaatatt caggaagtat ctcacaattt ttagcaaaag aacttcaaaa
     4681 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
     4741 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
     4801 tgtttcttcc tttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
     4861 aaatttctct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
     4921 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
     4981 tgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
     5041 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
     5101 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaagt
     5161 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
     5221 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     5281 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     5341 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     5401 ttgggacaat accattacaa attggtaaat aaacccattg ctttagttat gggtctagtg
     5461 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     5521 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     5581 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     5641 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     5701 aatttactaa cactgcgctc taataaaaaa caattagatt acacttttag tttggattct
     5761 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     5821 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     5881 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag cct
//
LOCUS       DACDLI010000109         5886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDLI010000109 DACDLI010000000
VERSION     DACDLI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.109"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..334)
                     /locus_tag="IV353_002518"
     CDS             complement(<1..334)
                     /locus_tag="IV353_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010707.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSY"
     gene            complement(327..764)
                     /locus_tag="IV353_002519"
     CDS             complement(327..764)
                     /locus_tag="IV353_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010708.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVEMKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            complement(780..1031)
                     /locus_tag="IV353_002520"
     CDS             complement(780..1031)
                     /locus_tag="IV353_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010709.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            complement(1031..1237)
                     /locus_tag="IV353_002521"
     CDS             complement(1031..1237)
                     /locus_tag="IV353_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9010710.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            complement(1686..1946)
                     /locus_tag="IV353_002522"
     CDS             complement(1686..1946)
                     /locus_tag="IV353_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010711.1"
                     /translation="MEHYTGLLDKIRVIKTQPLLVRFTLRTNHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2021..2224
                     /locus_tag="IV353_002523"
     CDS             2021..2224
                     /locus_tag="IV353_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9010712.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(2365..2850)
                     /gene="ssb"
                     /locus_tag="IV353_002524"
     CDS             complement(2365..2850)
                     /gene="ssb"
                     /locus_tag="IV353_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9010713.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFVNCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKATTESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(2853..3752)
                     /locus_tag="IV353_002525"
     CDS             complement(2853..3752)
                     /locus_tag="IV353_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9010714.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFADTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(3765..4358)
                     /locus_tag="IV353_002526"
     CDS             complement(3765..4358)
                     /locus_tag="IV353_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9010715.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(4447..4611)
                     /locus_tag="IV353_002527"
     CDS             complement(4447..4611)
                     /locus_tag="IV353_002527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010716.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMETKTAAI
                     KEPAADSKRN"
     gene            complement(4630..5052)
                     /locus_tag="IV353_002528"
     CDS             complement(4630..5052)
                     /locus_tag="IV353_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010717.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEMEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEARERVE
                     GKIKSNQTKKYSPQLKKPVNESVYARVDPFDPTKILKR"
     gene            complement(5054..5350)
                     /locus_tag="IV353_002529"
     CDS             complement(5054..5350)
                     /locus_tag="IV353_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010718.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLIENN
                     FDRPLMEYKKEKFDQLLEKFSEIQSYNTKILEEYVSQNNRILEILIEQNRGSEL"
BASE COUNT         1591 a         1162 c          928 g         2205 t
ORIGIN      
        1 tgtagctgac aaagttttcg accagttcac tcctttctag gtaaacattc atgatatatt
       61 tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcaggg cgacgtttat
      121 acacgtctaa ataattattg ctgactgtaa ccttcgtttg actcgtttca ctttccaaat
      181 acaattgttc ttcgactctt tgaataaagt acttcatttc ttccatacaa ctaaagtctt
      241 tttgaatccg tctgattact tcattcaaat gttctttgtt tgttaaaaag tgtgacacat
      301 cttccgttgt tagttgataa atcaccttat tcattttcgt tgtcctcctt tttcatgatg
      361 taacatgcgc catacttgtt gacattttca aagtagtcca accattcact tgaaacagtc
      421 ttggcttttt tcatttcgac aaatttccca tacgttagtt ctaaagcttg gtattcagaa
      481 ggtttgatag tgtttggctt gacgcctgct tccaagcata gtaaggcttg gaaaaaccat
      541 tgtccatttt gctttttcag ttcttcaagc gttcgatagt aggtcgcaat gagattcatt
      601 tcgacacccg attcaatcat ttcttccaca gctaccgaca atctttcctt ataatgtttt
      661 tttggaacac gtgtctcatt gtataagaat gtcatatcct ccattttttg tgccatttta
      721 tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt ttctcctttt
      781 tagttctcta atttggttaa ccaatattcg ttttcataag tttccatgtt ttcttgataa
      841 tgcagttcat atcctgctat cttcactagg tcttcaaata gcatagtcgg tgcatcctct
      901 actaaagggg tttctatcgt ccagtaccgc ggaataagta aagcttctgc tactttttca
      961 gaaatgactt ctttgtccac caaaatttga acgacttctt ttacagtcaa taatttgtgt
     1021 tgtgttttca tcagcgtttc tttcctttct catcaatcaa aatataatcg ccataagaca
     1081 aggtatattt tttcccgcat gttggacatt gatacaaacg atagcttttg atacatttcg
     1141 ctctttctcc acaatcgcat tcgacagtat gtggtgcatg atagtttggg aataattcgc
     1201 tgaagggaac accccaataa tcaaatttct ttcgcatgat tgattctgcc tcctcttagg
     1261 aaaggtttta cttcctttcg ttagttcttt ctttattgat ttgtttgcca gaatgaatca
     1321 acgaaaagcg acaaggacaa gggcgtgcag cgtgccattt acccttgtcc ttgtcgcttt
     1381 ttggtgataa aaggcaactt gttgccggtc tggcaaatca ataaagagat agcgaacagg
     1441 aacgaacctt ttggtgagag gaagcttttc ctattttgtc ttacaaagca agacccctta
     1501 tcttagtgaa cgtgcaacgt tcacttcctt tcttctcgaa cggcggtggc ggttggagtg
     1561 agagaaaggt ttttcaattg agaaggcgct tgtgccttcc taaacaggcc tttccctttc
     1621 ttcctgtttc tatgtaaaac aaaaagagct gcagtcctac attaggattg cagctcttct
     1681 tttatttata tgcccattga tcgcgtaaaa tgatcagggt ttctaatttg cattgattca
     1741 atgactagtt gatttcgttt attgaagtga cctgtcactg aaatagtata cttgccttct
     1801 tccaaaatca gcaatttatc cgctatttct atattggcca ccacacaatt aatcggttca
     1861 tgatttgtac gtaatgtaaa tcgcacaagc aatggctgtg tcttgattac ccgaatttta
     1921 tccaataaac cagtataatg ttccatcgcg atcacctcgc taaaattata gcaaaaacag
     1981 ctaaaattga gaatttttat tcgattttag atatgctata atgatcaaga cagtaaagtt
     2041 tgtgacggtg gctatccgat tgaaagtgag gtggtgcgta tgtgcgtact tccaaaatct
     2101 tacagaaagg agaagccttt tgcctgttga aacggcgcta ggattaatga ttagtttcgg
     2161 ttctttgatc gcaacaatca tttttggaat cttagcagtt gtaaacaaca atcaaaaaaa
     2221 ataaccgtca gctttagccg gtcgacgatt atttttcaaa aataatatta agctggtcac
     2281 cgtcttaaac ggtgctgtta ggggggttgt tagcgcaact cctcttttcg tatccattat
     2341 aacaacttta ttgatctttt acaatcagaa tggtaaatca tcatcactaa tatcgattga
     2401 ttgtccgcca aatggatttt gtgaagcgtt caatccattg ttgttaggcg tagctacatc
     2461 ccgttgttca aaggtggtag ggcttggact gctggacgat tgatcagcgt gcgcacggct
     2521 ctctgtcgtt gctttacttt ctaatagttg aaagttatca gctacaactt ctgtcacata
     2581 cacacgttgt ccttgttggt tatcgtaggt tcgggtttga atccgtccga caacgccaat
     2641 caagctgcct tttttagcta atgtcgccat cgtttctgct ggtttgcgcc aaatcacaca
     2701 gttgacaaaa tcggcttctc gattgccgtt ttgattcgtg aaattacgat taacggctaa
     2761 ggtaaaagtg gccactgctg tgccacttgc ggtataacgt aaatctggat ctttggttaa
     2821 ccgtcctact aaagttacat tattaatcat ctttatctct ccatttcttg attcatttgt
     2881 tgtgatcttt taaactgcct ttttctttct tgattttcaa ttttgatcac acttttcatt
     2941 aggtgatcaa tttcgttgac tgtatctgca aatgaaatgg aattcgcaaa cgtgttaact
     3001 gagcgatatt tttcccactg cttttccaaa tagtcgtttc ccctgatctt atccatttct
     3061 ctttcataat aaaaggataa aggctttgta tctccacgtt ttgtaaatgt atttcgaaca
     3121 cttttgtata actctttcgt atcaatattt tctagtttcg gaatggtaaa taagtcgtag
     3181 taatcttttg ctctcgaatt atctgtgcca aagctgaacg tagcacttag cttttctgca
     3241 ataatttgtt ctgttggata agctggaatc tccacttttt tatcttcaaa cattaattgg
     3301 tgatagtgtg tatctacttc aggataaata gaatctcctg ttgtcacgtc cattttaaac
     3361 ggtacacgta ctttctctaa attagccacc aatttaattt gaaatccagg ataaaaatcg
     3421 gcttctcttg tttccttcat tccttgaata gagaaaacaa tgccttcttt tgtgggtttt
     3481 ctagttaaat cattaaaaat gctcttcaat ttttcttcag ttaatttgct atttctaaat
     3541 gtcgtatcga tatcaattgt tgtccttcta tcgattccat attttgatcc taataaaaag
     3601 ccgcctttca aaatgaagtc atttttgtac tttgattcag aaatttgttc aattagacga
     3661 tccatcataa aatgccgttg cactaactgt gcatttaatc ctgtactctt tgctaaattt
     3721 cttgtttttg ctgcaaattg tgtcggtgtc atctttttcc tccatcaata taagctttgc
     3781 atgtggtatt tcatttcttt ttctacatgt agttttgtca tgtaatctct taatttactt
     3841 ggatctctca atgggtcttc catgtactct tttaatgctt ctaatttcat ttcatattca
     3901 atttcatatc tcggattcca aatatcacaa agtgtacgct ctttatcata catagtaata
     3961 ggatttcctt gaaaggaagt tccctctact tttcctagtt cataaacttc tttcttcacg
     4021 aaatgaaatt gtaactgctg ctcttttgta attgaacgat caacgttata gccatgggga
     4081 aaagttaagt gaatgaattt cggcatattc gtagaaagct ggtaaatatc taaagtcgtt
     4141 tcatgagaga agattcccct ggaaaatcgt tgctgaagcc aaaataaatc atctattcca
     4201 tgaccatcct ctacataata accacgatca actttgatca attttccttg attcaccaat
     4261 ctttgtacag tggttgctgc gattcctaat tttctggctt ccttcataga taaatttcca
     4321 tcgcgaatag ccaacaattc tttcagtttc tcttgcattt acttcccccc cttttgttac
     4381 aaatacacta cattaaatat atcatgatag tgtatttgta acaaggttaa tgtctatcga
     4441 tctaattcaa tttctttttg aatccgctgc tggttcttta attgctgcag tcttcgtttc
     4501 catttcttcc agcgcttttt ctcttgtcat ataatcaatg tatgttgaaa aagatttttt
     4561 attgggcaag gtaaattgac aagtgagaat aactgcaggc gatttattca tcatgttccc
     4621 cttctacttt tatcttttta gaatcttcgt tggatcaaat ggatctactc tggcataaac
     4681 agattcattc acaggtttct tcaactgagg ggaatatttt ttggtttgat tagatttaat
     4741 ttttccttcc acacgttctc ttgcttcttg gtaaaccgtt gaatttgtac caattagtat
     4801 tcctgtatct ttcaaatgaa gatctgcaaa ctctgaaacc atttcggtta aaatagaaat
     4861 ttctttactc attccattca actttgcttg attggaattt acttttttct ccatttctgg
     4921 atcataattc atacatagaa accgaacggc ttcactatag tttttaactt ctgcatgagt
     4981 agaaataatc tcatccaaca catcgatatg tgtttggtga aagtaaatgt gtttgtctat
     5041 aacctttgcc atatcaaagt tcacttcctc tattttgctc gatcaagatt tctaaaatac
     5101 gattgttttg gctaacatat tcttctaata tctttgtgtt atagctttga atttctgaaa
     5161 acttttctaa taactgatca aacttttctt ttttatattc catcaaagga cgatcaaaat
     5221 tattttctat tagtaatttt aaataatcgt tacgactcca tttattaccc gtacggttac
     5281 ttagttcgtc acatcttcga tcaatttctc ttacgatttt ctcatccaca tttcttattc
     5341 ttacttccat attaattcct ccaatttaaa aaggatagcg aaaaggagcc tatccttttt
     5401 gctatcctac taccctcctt ttactatcct tcactatcct ttcactatcc tatttttaaa
     5461 tattttgttc ccacatttat ttttgagaaa cgtaataggc acagcctatg ggaacacttt
     5521 ttttaaaaag tgttcccacg tttccttatg ctctttacaa ataaaggctt ttagagacgt
     5581 tctggaattt tagattcgtt gaattgttcc cattcatcaa tcaattggct ataagtagcc
     5641 attaattttt cttgctcttt cttaaaattc cgaaacaaca aatattactt tgtaatattt
     5701 gtaattaaat tagaatcgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     5761 gttacactca aattgtaata cccgctaaag catacaagga gagtgaacat aaatgactta
     5821 tacccatctt acaccaaacg agcttataat gatagaagca tattttcatc aagaaactcc
     5881 ggttgc
//
LOCUS       DACDLI010000110         5785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDLI010000110 DACDLI010000000
VERSION     DACDLI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.110"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            84..512
                     /locus_tag="IV353_002530"
     CDS             84..512
                     /locus_tag="IV353_002530"
                     /inference="COORDINATES: protein motif:HMM:NF025054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9010719.1"
                     /translation="MIEGYSVIKSFDILENKKRINQILLEELAEKKSIYKREEKEISF
                     SYFIENDYAGGIVGKVIFSTCYIDLFAVKKKYRSKGIGRELFNTVENYCRINSVKYIY
                     LTTQDYQALEFYLHFGCKVFGELPDVPFKNTTKFFLIKII"
     gene            complement(652..1509)
                     /locus_tag="IV353_002531"
     CDS             complement(652..1509)
                     /locus_tag="IV353_002531"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9010720.1"
                     /translation="MEDFWYHNKSVSAPISLSQCGYESYHPNSSIRNYIVQQKWIFHY
                     VLSGKGFLEVEAQHFELKEHDIFFFFQGQKVKYYTDKKEPWTLIWLGIQGDKTSEFLK
                     ETTLLNTHTVSLTKNMNKKIKNILLKIVCENRELIDEPETLKELRNIASIYDFLYHIK
                     KTFLAPSLITNKPTQVNIIDYINNHYMNDISPKKIAQYFNISYSQLYKLCISKFNSSP
                     KKIITNHRIRVAVDLLIDSDNTIKEIAQKVGYKDEFLFSKTFSKTLGYCPSKFRKLNK
                     DQLKNIRFK"
     gene            1652..3004
                     /locus_tag="IV353_002532"
     CDS             1652..3004
                     /locus_tag="IV353_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9010721.1"
                     /translation="MIDKIMNTIQNKVLPIATKIGNQRFLVALRDSFIGTMPIVMTGS
                     IALLINAFLVDIPDQFNLQKITSFFQWWVDINNLVFKGSISIVALLFIYCLGVNIAKI
                     YKTDTLSSGLVALSSFIISIGNSMTSTYNLSNNNIDLTTLFTGVEGIKVTGSSLNVTI
                     SGLLPGTQINSNGYFTAIIIGFLSSIIFCKLMNKNWTIKLPDTVPPAIAKPFLSIIPA
                     LVSLYIVAIFTFSFTKITDLLIIDWIYKVLQTPMLGLSQSFLAVVLVAALTQFFWFFG
                     IHGGNVMAPIMEGVFGVALLANLEAYQNNETIPYVWTSVSYGSFVWYATLGLLIAIFW
                     VSKNSHYKEVAKLGIMPVLFNIGEPVMYGLPTVLNPILFIPFLLCPAVMSSVAYLVTD
                     LGWVSPVTQNVTWVMPPVLYGFFSTGFDWRSIILSIINLFLATLIYLPFVRMANKKIG
                     "
     gene            3082..4917
                     /locus_tag="IV353_002533"
     CDS             3082..4917
                     /locus_tag="IV353_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAP9010722.1"
                     /translation="MQTFEIKEEFLVDGKPTKLISGAIHYFRMTPAQWEDSLYNLKAL
                     GANTVETYIPWNLHEPVEGIYDFEGMKDIVAFVSLAQELGLMVILRPSVYICAEWEFG
                     GLPAWLLKEHVRLRSTDPRFMAKVRSYFSVLLPKLVPLQVTHGGPVIMMQVENEYGSY
                     GMEKEYLRQTKQLMEEFGIDVPLFTSDGAWEEVLDAGTLIEEDVFVTGNFGSHSKENA
                     AVMKAFMAKHDKKWPIMCMEYWDGWFNRWGEPIIKRDGQDLANEVKDMLDLGSLNLYM
                     FHGGTNFGFYNGCSARGVLDLPQVTSYDYDALLTEAGEPTEKYFHVQRAIKEVCPEVW
                     QAEPRRKTFGSLGTFPVQNSVSLLAVKDQMMTAQETMYPITMEEAGPGYGYMLYSVNL
                     KNYHHENKLKVVEASDRLHLFADGSLQTIQYQENLGEEVMIKGTPEKEWIELDVLVEN
                     LGRVNYGFKLNGPTQVKGIRGGIMQDIHFHQGYRQYALTLSADQLKKIDYTAGKNPAQ
                     PSFYQVEFTLTDLADTFIDCRSYGKGVVIVNGINLGRYWQRGPIHSLYCPKEFLKKGT
                     NEIVIFETEGIEINELIFCGQPIVKKLLTNDFSEIGSNIHNHIDS"
     gene            5486..>5785
                     /locus_tag="IV353_002534"
     CDS             5486..>5785
                     /locus_tag="IV353_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9010723.1"
                     /translation="MQQNNNEISTQHQFDAYCKKVLRNEAKSIRKRNDKIKENEEPLN
                     DLNEGKYSQTHLDEQDVYFLFGMEILISDQKLSAAIDQLSDTRKKIVLLYYFAGFN"
BASE COUNT         1902 a          789 c         1086 g         2008 t
ORIGIN      
        1 atgcgcgttt gataggtaaa atgtttaaat aatcactttc tatatttcta aagttaaatc
       61 taagttattg aagtatttat tatatgattg aaggttatag tgtgattaaa agttttgata
      121 ttctagaaaa taaaaaaaga attaatcaaa tattgctaga ggagttagct gagaaaaaat
      181 caatttataa aagagaagaa aaagaaatat cattctcata ttttattgaa aatgattacg
      241 caggaggaat tgttgggaaa gtaatattct ctacttgtta tatcgattta ttcgctgtaa
      301 aaaagaaata taggagtaaa ggtattggaa gagaattatt taatacagtt gaaaattatt
      361 gtcgaataaa tagtgttaaa tatatttatt taacaactca agattatcag gctttggaat
      421 tttatttaca ttttggatgt aaggtatttg gagaattacc tgatgttcct tttaagaata
      481 caacaaaatt ttttttgatt aagataatct agtactatat atgagctata acattaactt
      541 gttaccttta aaaattagtt gaaagcagaa atatcattaa gcgtaataat agttgaagct
      601 agtttaagta gggaaataac tagtttcaac tattttttaa acgtgtattg tttatttaaa
      661 tcttatgttt tttaactgat ctttattaag ctttctaaat ttagaagggc agtaacctag
      721 agttttggaa aaagttttag aaaataaaaa ttcatctttg tatccaactt tttgagctat
      781 ttctttaatt gtattatcgg aatcaattag tagatctaca gcgactctaa ttctatgatt
      841 ggttattatt tttttaggac tagagttaaa tttagatata catagtttat atagctgact
      901 gtaacttatg ttaaaatatt gagctatttt tttaggagaa atatcgttca tataatggtt
      961 attaatatag tcaataatgt taacttgagt tggtttattt gtgattaaag aaggtgccaa
     1021 aaaagttttt ttaatatgat ataaaaaatc atatatactt gctatatttc ttaattcttt
     1081 taaagtttcc ggttcatcaa ttagttctct attttcacat actattttca atagtatgtt
     1141 ttttattttt ttattcatat tcttagtcaa gctaactgta tgagtattta gtaaagttgt
     1201 ttctttcaaa aattcagaag tcttatcacc ttgtatacct aaccaaatca gtgtccaagg
     1261 ttccttttta tctgtataat acttcacttt ttgaccttga aaaaaaaaga aaatatcgtg
     1321 ttctttaagt tcaaaatgtt gagcttctac ttctaagaat cccttaccag ataaaacata
     1381 atgaaatatc catttttgtt gaactatata attacgaata gaagaattgg gatgataaga
     1441 ttcatatccg cattgggaaa gggaaattgg agcagaaact gacttattat gataccaaaa
     1501 atcctccatt attatcctcc tctatagaac taattatgaa gaaaatacca tgttttaagt
     1561 ataaattatc atgttcaaat ggtcaaaaaa atttatatac tataactgga tagcgctttc
     1621 taattttttt ctttttgaag gagtgaatgt gatgattgat aaaataatga atacaattca
     1681 gaataaagtt ttacctatag ctactaagat tgggaatcaa cgatttttgg tagctttaag
     1741 agattcattt atagggacaa tgcctatagt aatgacaggt tcaattgcgt tgttaatcaa
     1801 tgcttttttg gttgatattc ctgatcagtt taatttacaa aagattacgt cgttcttcca
     1861 atggtgggtg gatattaata acttggtttt taaaggtagc atttcgatag tagctttgct
     1921 ttttatttat tgtttaggag taaatattgc caaaatatat aaaacagata ctttgtcttc
     1981 aggactagtc gcactgtctt catttattat ttccataggt aattctatga cttcaactta
     2041 caatttatcg aataataata ttgacttaac aacactattt actggggttg agggaataaa
     2101 agttacaggt agcagtttaa atgtgactat atcaggattg ttgcctggta ctcaaattaa
     2161 ttcaaatggt tattttactg caataatcat aggtttttta tcttcaatta ttttttgtaa
     2221 actcatgaat aaaaattgga caattaaatt acctgataca gttcctccag ctattgctaa
     2281 accattttta tcaataattc cagctctagt ttcattgtat atagtagcta tttttacttt
     2341 ttcattcact aaaattacgg atttattaat tatagattgg atatacaaag tattacaaac
     2401 accgatgtta ggcttatctc aaagttttct tgcagtggta ttagtggcag ccttaacaca
     2461 atttttctgg tttttcggta ttcatggtgg aaatgttatg gcgcctatta tggaaggtgt
     2521 atttggagtg gctctattag ctaatttaga agcttatcaa aacaatgaaa ctattcctta
     2581 tgtttggaca agcgtatcat acggatcgtt tgtctggtat gcaactttag gtttattaat
     2641 tgcgattttt tgggtatcaa aaaatagtca ttataaagaa gttgcaaaac ttggaattat
     2701 gcctgttttg tttaatattg gtgaaccagt aatgtatgga ttacctactg tattaaatcc
     2761 tattttattt attccatttt tgttatgtcc tgctgtaatg tctagtgtgg cttatttagt
     2821 aacagattta ggttgggttt ctccagtaac acaaaatgta acttgggtaa tgccaccggt
     2881 cttatatgga ttcttttcga caggatttga ttggagatca attattttat caattattaa
     2941 tttattttta gccacgttaa tttatttgcc atttgttaga atggcaaata agaaaattgg
     3001 ataatttttt attaagtcgt agttagtgtg tattagaagt atgataagcc tcatttttga
     3061 tatattaagg aagtagaaga catgcaaacg tttgagataa aagaagaatt tttggtagat
     3121 ggaaaaccaa ctaaattgat tagtggcgcg atccattatt ttcgcatgac accggcacaa
     3181 tgggaagata gtttatacaa tttgaaagca ctgggtgcga ataccgtaga gacttatatt
     3241 ccttggaatc tgcatgagcc agtggaaggc atctatgact ttgaggggat gaaagacatc
     3301 gtcgcttttg tgtcattggc gcaagaactt ggcttgatgg tcattttgcg gccatctgtc
     3361 tatatttgtg cggaatggga attcggcggg ctacctgctt ggttgctgaa agagcacgtg
     3421 cgattgcgtt caactgatcc acgatttatg gcaaaagtcc gttcttattt cagtgtgctt
     3481 ttaccaaaat tagttccttt gcaagtgaca catggcggac cagtcatcat gatgcaagtc
     3541 gaaaatgaat atggttctta tggtatggag aaagagtact tgcggcaaac gaaacagttg
     3601 atggaagaat tcggtattga tgttccgtta tttacgtcag atggtgcttg ggaagaagtg
     3661 ctggatgcgg gcacactgat tgaagaggat gttttcgtaa caggaaattt cgggagtcat
     3721 tccaaagaga acgctgcagt gatgaaggca tttatggcaa aacacgacaa aaagtggccg
     3781 attatgtgca tggaatactg ggacggttgg ttcaatcgtt ggggagaacc gattatcaag
     3841 cgtgatggcc aagatttagc taatgaagtc aaggatatgt tagacttggg ttcattgaat
     3901 ctctatatgt tccatggcgg aaccaatttt ggtttttata atggttgttc tgctcgtggt
     3961 gtgttggatt tgccacaagt gactagctat gactacgatg cgttattgac ggaagctggc
     4021 gagccgactg agaaatactt ccatgtacaa agagccatta aagaagtatg tcctgaagtg
     4081 tggcaagccg agccccgcag gaaaacattt ggttctttag gaacattccc tgtgcaaaac
     4141 agcgtctcac tgttggcggt aaaagaccag atgatgacgg cccaagaaac gatgtatcca
     4201 ataacgatgg aagaagcagg acctggttat gggtatatgc tttattcagt aaacttaaaa
     4261 aattatcatc acgaaaataa gctcaaagtt gtggaggcca gtgaccgatt gcatcttttt
     4321 gcagacggta gcctgcagac aattcaatat caagaaaacc ttggggaaga agtgatgatc
     4381 aagggtacgc cagagaaaga atggattgaa ctagatgttt tagtagagaa cttaggccga
     4441 gtgaactatg gctttaaatt gaatgggccg acacaagtga aaggcattcg tgggggaatc
     4501 atgcaagaca tccattttca tcaaggctat cggcaatatg cattgacatt gtcagcagat
     4561 caactcaaaa aaatcgatta tactgccggt aaaaatccgg cccagccttc tttttatcaa
     4621 gttgaattca cattgactga cctcgcggat acatttatcg attgccgcag ctatggcaaa
     4681 ggggtggtca tcgtcaacgg tatcaactta ggtcgttact ggcaaagagg accgattcat
     4741 tccttgtatt gtccgaaaga gtttttgaaa aaaggcacaa atgagatcgt gatttttgaa
     4801 acggaaggga tagaaattaa tgagctcatc ttttgtggac aaccgatagt caagaaactt
     4861 ttgactaatg atttttctga aataggttca aatatccata accatataga cagctagttt
     4921 aaatttattg cagcaaacca atcattgtat gtgtcgcgat ttttccaaat ctcaatactt
     4981 attctaacgc atcacaaata tttatctcaa caagtctcga atgattttta cttttcattt
     5041 atggttcaag aagtgaatag tttacacttt cttttgagat tgacttatat tcgcgataaa
     5101 catcctaaat gatagtagat aatttaagca tttgatcgtt gataaaaggt ataattatag
     5161 atttatcaca gatacattgc ttttttcgaa gaaactataa aaaatgagag tctaggttat
     5221 ttgagaatat caattgagca agtacaaata actccaaatt tagtaatatt cctaagtgat
     5281 tattggtgag taattaaaaa ggaatactca agctacatgt atagcataga aaatcagcat
     5341 tcaatcagac aattaggatt gaatgctgac ttttttgtta tattgatatt tttaattatc
     5401 ttctttattt tcttctagtt tttcgaaaaa taggtattct ctgattgttg tattagtgga
     5461 aaggggataa aggagcatat tgatcgtgca acaaaacaac aatgagatat ctacacaaca
     5521 ccaatttgat gcctactgta aaaaagtatt acgcaatgaa gctaagtcta ttcgaaaacg
     5581 aaacgataaa ataaaagaaa atgaggaacc attaaatgat ttaaatgaag gtaagtattc
     5641 acaaactcat ttagatgagc aagatgttta tttcttattt ggaatggaga tattaatttc
     5701 agatcagaag ttatcagcag caattgatca attatcggat acaaggaaaa agattgtttt
     5761 actttattat tttgcaggtt ttaat
//
LOCUS       DACDLI010000111         5743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDLI010000111 DACDLI010000000
VERSION     DACDLI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.111"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..183
                     /gene="rrf"
                     /locus_tag="IV353_002535"
     rRNA            68..183
                     /gene="rrf"
                     /locus_tag="IV353_002535"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            193..266
                     /locus_tag="IV353_002536"
     tRNA            193..266
                     /locus_tag="IV353_002536"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Asn,seq:gtt)
     gene            306..395
                     /locus_tag="IV353_002537"
     tRNA            306..395
                     /locus_tag="IV353_002537"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:340..342,aa:Ser,seq:gga)
     gene            410..481
                     /locus_tag="IV353_002538"
     tRNA            410..481
                     /locus_tag="IV353_002538"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:443..445,aa:Glu,seq:ttc)
     gene            497..569
                     /locus_tag="IV353_002539"
     tRNA            497..569
                     /locus_tag="IV353_002539"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:530..532,aa:Val,seq:tac)
     gene            579..651
                     /locus_tag="IV353_002540"
     tRNA            579..651
                     /locus_tag="IV353_002540"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            687..762
                     /locus_tag="IV353_002541"
     tRNA            687..762
                     /locus_tag="IV353_002541"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:720..722,aa:Phe,seq:gaa)
     gene            764..844
                     /locus_tag="IV353_002542"
     tRNA            764..844
                     /locus_tag="IV353_002542"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:798..800,aa:Tyr,seq:gta)
     gene            851..924
                     /locus_tag="IV353_002543"
     tRNA            851..924
                     /locus_tag="IV353_002543"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:883..885,aa:Trp,seq:cca)
     gene            927..999
                     /locus_tag="IV353_002544"
     tRNA            927..999
                     /locus_tag="IV353_002544"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:His,seq:gtg)
     gene            1011..1082
                     /locus_tag="IV353_002545"
     tRNA            1011..1082
                     /locus_tag="IV353_002545"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1043..1045,aa:Gln,seq:ttg)
     gene            1128..1198
                     /locus_tag="IV353_002546"
     tRNA            1128..1198
                     /locus_tag="IV353_002546"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1160..1162,aa:Cys,seq:gca)
     gene            1219..1303
                     /locus_tag="IV353_002547"
     tRNA            1219..1303
                     /locus_tag="IV353_002547"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1253..1255,aa:Leu,seq:caa)
     gene            1365..1437
                     /locus_tag="IV353_002548"
     tRNA            1365..1437
                     /locus_tag="IV353_002548"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1398..1400,aa:His,seq:gtg)
     gene            1447..1518
                     /locus_tag="IV353_002549"
     tRNA            1447..1518
                     /locus_tag="IV353_002549"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1479..1481,aa:Gln,seq:ttg)
     gene            1536..1620
                     /locus_tag="IV353_002550"
     tRNA            1536..1620
                     /locus_tag="IV353_002550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1570..1572,aa:Leu,seq:caa)
     gene            1875..2750
                     /locus_tag="IV353_002551"
     CDS             1875..2750
                     /locus_tag="IV353_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9010724.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2854..3618
                     /locus_tag="IV353_002552"
     CDS             2854..3618
                     /locus_tag="IV353_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9010725.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3635..5047
                     /locus_tag="IV353_002553"
     CDS             3635..5047
                     /locus_tag="IV353_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9010726.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            5064..5743
                     /locus_tag="IV353_002554"
                     /pseudo
     CDS             5064..5743
                     /locus_tag="IV353_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738748.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
BASE COUNT         1741 a          970 c         1293 g         1739 t
ORIGIN      
        1 aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat aaaagataac
       61 accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga acacagaagt
      121 taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag gacgtcgcca
      181 cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta atcatgatgt
      241 cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg agcttctttc
      301 actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt agatggtgcc
      361 aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg gcccgttggt
      421 caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc cgtacgggtc
      481 attttaattg aatgttggag gtttagctca gctgggagag catctgcctt acaagcagag
      541 ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gtcgttcttg
      661 ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag caacggattg
      721 aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag tagcgaagtg
      781 gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa tccactctcc
      841 tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc gttagtgtgg
      901 gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg caccggattg
      961 tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct tggggtatag
     1021 ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc cagctacccc
     1081 agtttctata atttattctt tattttgtag attgataata gtataatggc ggtatagcca
     1141 agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt taccgcctta
     1201 gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga ctcaaaatcc
     1261 agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta agatgtcttc
     1321 atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat gtggcgaagt
     1381 ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc attcgccctt
     1441 tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
     1501 cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa ttggcagacg
     1561 cgctggactc aaaatccagt gtccgttgag gacgtgccgg ttcgaccccg gccgccggta
     1621 taattatcaa tatagcaact ttcttataga gagttgctat attttttttg tttttatgta
     1681 attacctcca aatcaattcg atacaagtat tatcataaaa cgagtaaaaa agatattgat
     1741 aacgctttta ttatgtgtta aactattttt gtaaattatg tagggtgcta ctattcaatt
     1801 aggcacgacc taagaaaggg ttctttctta ggtcgttttt tgtctttaaa aattgtttgg
     1861 agacaaaaat atgtatgaag gagtctgcaa gttgttttgt tttatcatat agtggaggtg
     1921 aattaacggt gtatgtgatg aaaaagctga atcataatgc tgtattgatc gatgacgatg
     1981 gaatagaaaa aattgcgatg gggaaaggga ttggcttttc tgctaaagtg agtaagccat
     2041 ttgatgttga ggatgctgac aaattatttg tattagattc taaagaaaag atcaaacgat
     2101 tcagtgagat agtctcgcag attccaatcg aatttatgga ttttgcggaa gaagtgattg
     2161 aactgataac agctaggatc tcctctccat tagattcaaa tatctatatt acattgacag
     2221 atcatattta ttttgctatt caacgaaaaa gagaagatag agatgtcaca gcaattatgt
     2281 tgccagaaat gagattcctt tatccaaatg aatatgatat agcaatagaa gtggtcgagc
     2341 gaatcaatca tcgatatcaa accgagttag ggtcaaatga agtaggattc atcactatgc
     2401 atatcattaa tgctgagcta ggagagccta atagtttaaa gagcttaaaa atagtagaaa
     2461 tgacgaccac gattttgtcc catctagaaa aatcctattc aaacgtttat gataaagaat
     2521 cactgactta tcatcgctta atggttcata tcaaattcct ggttaaacgc ttgatttatc
     2581 aggaagaaac gccagtagaa aatattggtt tttttactga aacatttaaa catagcaaag
     2641 cgtattgtac agcactaaag attaaagata tcatcgaaca gcaatttcaa cttgccattc
     2701 aagaaaatga aatcatctat ttggcaattc atctagcacg aatctattaa aataagggcg
     2761 taactatcta taggcgcaac caaatgacac atagtaaatg gactatgatt atttggttgc
     2821 gcctatttta gtagaaaaaa gaaggagaat gaaatgggaa aagtgatttt taattcggat
     2881 gattttggct atagccatgg tgtgaattat gggattatgg atgcttatca gcgaggaatt
     2941 ttgacatcaa ctacattgat ggcaaatatg ccgggtttcg agcacgctgt gaaactaaga
     3001 aaggaaatgc ctaggctagg tgtaggagtc catttaacat tgacgtgtgg caaaccactt
     3061 ctcaaaactg tcgatactct tatcgaaagc ggagagttca gatccttatc ttattatcag
     3121 catccattta acattgacaa agatcaactg tatcaggaat ggaatgctca gattcaaaaa
     3181 atttatcatg cggggatcat accaactcat ttagacagtc atcatcatac tcatactttg
     3241 gatgaaaacc aggaggtagt tgttacatta gcaaaaaaat atgatttgcc ggttcggagc
     3301 aactttgaac gaaaagatga agttcgtcat gtcgattatt ttgagccttg tttcgatgtc
     3361 gtaggagaaa ctgatgaaaa aaaacgacaa atcaatacga gtgtggaaga atatttggca
     3421 agtttactca tgatattgag agaagataaa acgatcgaag tcatgtgtca cgcagcgtat
     3481 ttggatcagg aattgttaaa tggttcaagt tttgtgtatc ctcgtactca ccaagtcgaa
     3541 tttttaattc attcagcatt tgctcaaaaa gtgaaaaatg atcctttgat tgagttagta
     3601 acatatgagg atatttaata gaggaggaat aaaaatgaat gttaaatcaa atgtgcagaa
     3661 gcttggaaga gcgatgctac ttccagttgc agcgatgcca ttagcgggat tgatcatgcg
     3721 tttatcagct gatgatatgc tgaacatccc agtaattggt gcagctggaa atgccgtttt
     3781 tggaaacttg gatatcttat ttgctatagg agttgcaatc ggttttgtca aaaataagga
     3841 caaaggaata cctgcactta ctggtttttt agcgattgct accttgaaaa aaggcttaga
     3901 aatcatggac ccagaagtga atatggggat ttttggtgga attatttccg gtttagtagc
     3961 ggcatggacg tataatcgct ttaaagaaca aaaattacca gtagtttttt cttattttgc
     4021 gggcgaaaaa ttcccattga ctatggtgat gattctacaa acaatcgcag ctattttttt
     4081 gggctttatc tggccgctca tccaaattgg gattgataat tttgcaaaac tgctcgttga
     4141 tatgggggca ttaggtgtgg ggatatttat gtttttgaat cgtttgctga tcccctttgg
     4201 actgcaccat gtgttgaata cgtatattta ttacgattta ggaagctata catctcctag
     4261 caaggaagtt ttcagaggag agatgacacg ctttatcaac ggagatcctc aggctggact
     4321 gtttttatca ggtttttttg tagtaatgat gtttggtata ccagctatat gtctagctat
     4381 ctataaagca gcttataaag aaaataaaga gatggtcaaa ggaatcatgg gaagcggtgc
     4441 aggaacttca tttattgcaa atatcactga gcctgttgaa tttagtttca tgtttctttc
     4501 tcctatgttg tacgtgatcc atgcactttt tgccggttta gcagggattg tctgttactt
     4561 attcaatata cgaattggtt ttacattcgg agcatgtatt gttgattatc tcatcaattt
     4621 tcgaatcgct accaatgcta ttttgattct tccaatcgga attttctttt ttgccttgta
     4681 ttacgttact ttttattatt tgatcaataa gagaaacatt cagacattag gacgtgaagc
     4741 taaggcagag tttggcaatg aagtaacctt agaagaaaca gagttagggt tagcaagtaa
     4801 aaattatgaa tatatggcaa caaaaatgct acaagcgttt ggaggaaaag ccaatatcct
     4861 ggatgtctat agttgtaata cacgcctacg ggtagaggta gtggatccca ccatggtcga
     4921 ggaacagcga atcaagcaat tagggataag cgggatcatt aaaccaactg aaaaaaatta
     4981 tcaaattatc atcggcttag aagtgactta tgttatggca gaattcaata aattattaga
     5041 agaatagaaa ggaaaagatg atgatgaaaa aacagattat cactatagct ggaggcggga
     5101 gtacgtatac accaggaatc gttcaagcca ttttgaacaa tgaaaaacgg ttgccattat
     5161 ctgaaatacg tttatatgat attgatgaag aaagaaatat ggatatgtac ttgattgtta
     5221 aatttatgct gaaaaaaaag gtttttcaaa tattcgcatt cgtgcaacgg atgatccaaa
     5281 gcttgctttt acaggatgcg attttgtttt ctcacaaatt cgtgttggag gattggagat
     5341 gcgagaaaag gacgaaaaga ttccacttaa acatggatta gtaggacagg aaacgtgtgg
     5401 actcggagga tttgcttatg ggatgcgctc aatcaaaggt ttattggaaa ttgtcgatca
     5461 tatacaagac tatgccccca aagcatggat tttgaattat acaaatccag agtcgatcgt
     5521 ttctgaagct gttcgtcgta aatatccgaa tgtaaaaatg attaatgcct gtgatatgac
     5581 tatttcaatt gaagaaacga ttgcagtgaa ttacggttat gatcgtaaga attggatcgt
     5641 tacgtattat ggtttaaacc actttggctg gtatacatcg atttatgata aagaattgca
     5701 acgagaaatt atgccagaga ttattgaaaa actaataaca aaa
//
LOCUS       DACDLI010000112         5691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDLI010000112 DACDLI010000000
VERSION     DACDLI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.112"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(490..1299)
                     /locus_tag="IV353_002555"
     CDS             complement(490..1299)
                     /locus_tag="IV353_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9010727.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1299..2045)
                     /locus_tag="IV353_002556"
     CDS             complement(1299..2045)
                     /locus_tag="IV353_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9010728.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2063..2530)
                     /locus_tag="IV353_002557"
     CDS             complement(2063..2530)
                     /locus_tag="IV353_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9010729.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2530..2940)
                     /locus_tag="IV353_002558"
     CDS             complement(2530..2940)
                     /locus_tag="IV353_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9010730.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2951..3964)
                     /locus_tag="IV353_002559"
     CDS             complement(2951..3964)
                     /locus_tag="IV353_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9010731.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4181..4915
                     /locus_tag="IV353_002560"
     CDS             4181..4915
                     /locus_tag="IV353_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9010732.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4899..5609
                     /locus_tag="IV353_002561"
     CDS             4899..5609
                     /locus_tag="IV353_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9010733.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1833 a         1195 c          943 g         1720 t
ORIGIN      
        1 taaagcgaca aaaaccacga gcaggtccat tcatcctcaa acctcggtga tcttcgcggt
       61 ttaacaccat cagaagaaat gcacaaaaat aataaggaga gtctgagaca taaataaatt
      121 cccagacttt tagtgagtct aaatccgaat aaacggtggg tcgaaagcaa ctctagctgc
      181 gcgtcgatcg cagcaatctc taggtgctat gttcggataa atggtggtca aaagtcagat
      241 tttcggccat ttcgtaacag ccaaccagac aaaaagcgtc tgttttgtct tctactccaa
      301 ttgctcaaat cttagcgact tttggcttgg tcttactcta aacagtttgt aagcctaaac
      361 tttatttgct gtttgtttct aagatcaaag caaaactact ttttagttgg ttacttttac
      421 aagttctttt tactgttgaa taaatgtaaa tcttaagaac aaatatttac ttctataatt
      481 ccaagtttct taaaaaattc cgccaagtgt acctaaaaag cataaaacaa aaattcctag
      541 aataattgtg atcgttttat gccctttgga gtaaagccag acgcataaaa aggtaattcc
      601 taatggcagc aagcccggaa aaatttgatc aaaaatactt tgtaatgaag tttcagctcc
      661 accaaccgtg aattttagtg acgtatttaa agtaaccatc gtgcctatca tcgcgccaat
      721 taccgttaaa cctaaaatcg aagcaccatg agataatttg tcaatcatat ggttgtcttc
      781 tgtatcatca ataaatttag ttcctaaaac atagccatat tttgtcgcca accatttaaa
      841 aataaaatga atggcagtat acaaagtaaa ataaaggatc atgcctaaaa tattcccttg
      901 ttgagcaaaa gaaataccta tgccagccgc aataacccga aaagttcccc agaaaaatga
      961 atcgccaata cctgataaag gtcccattaa tcctgttttg attttattaa ttgcgtctgt
     1021 atcaaattca ggatttttag cattttcttc ttccatggtg acggacaagc tcataacaaa
     1081 aggcgcaata tgcggtgtaa tgttaaatgt ttccgtatgg cgctgcaacg ctgtataata
     1141 atcatcatcg ttcggatata ttttacgtaa ggaacgttca ataccattca tttgtccata
     1201 tgccatcatt ttatcgaagg accaagagga ttgcataaaa aaggagcgcc aaaatatact
     1261 tcttaattct tttttcccaa taacactttc ttttaccatt ataaatcttc ctcctcttca
     1321 aaagttagct tttgaacagc cagtaacgct aagcaaagtc cgacgacagc cacaccgaca
     1381 acatccattt tcagatacat tacgccgaca aatcctaaga aaagataagg catcatcgct
     1441 ttattcgcca tcattttaat taataacgct agcccaacac aagaaagcac accggccgca
     1501 acatcaaagc tgtccaatac aacctgagga atcaacttga tcatcgcatt gattgtagac
     1561 acaccaaaat aagtagctac aaaaactaaa atggccgatg gaaccccaac gattaaaggt
     1621 tgagccacat aatgatattt ttgtgctttt ttgaaatcat gattgactaa tgcctcatcc
     1681 attttgttgt taaaatacgt aattggcatt gcataggcga agttacgcca catctgaaga
     1741 aagatggaca aaggtaaagt caacgcaacg gcagttccta ctccttgacc cgattgaatc
     1801 gcaattgctg tacctaaaac actggaagca taaacttcag ggggcgtcgc actccctacc
     1861 ataattgagc ccatgaacat ggattccaag gtggcaccaa taataattcc tgactctaaa
     1921 tctcccaaaa tcaaaccgac aaaaggcgct gtaaatagcg gtctacccat catcgatgca
     1981 ccaaatgtgt attcatcaat agacgccaaa aaagctacca gtgcgattaa tattgcttgt
     2041 atcatattct tcccctcctc ttttagtcat gcaaaggatt gatttttttg tccgttggta
     2101 cagcctgaca ttgaactgtt atcccgtttt cagtcatttt tttcaccaag tccagttctt
     2161 gttcattcaa gtaaacggaa ctagtaatca ttttccgatt ctcgtttttc tttaaatttc
     2221 ctaaattcac ttctttaatc tcaggtacgt gctgagtaat ttcgtaggca tcctccaccg
     2281 tcccgacaat taaaaatact tggtatttct tgattttttc tccatttaaa acagagatgg
     2341 cctctgtcat atccttcacc acaaattttg tccctgtagg aacagccatt tttagtgttt
     2401 ttttgcgcag attattggta gcgatttcat tacttgcaac gaaaatacaa tttgcaccta
     2461 agttatttgt ccaggcaaaa gcaacttgcc catgaacaaa acgttcatct actcgtaata
     2521 atttaatcat taaaaatcat cctcttctgt tttctctagc aactcgttca ccaaaactat
     2581 cccttcattc ccgctattca tagctttttg gattagtttt ttcgtgtcct gttcacctga
     2641 taagcaaagc tctaaaagca gcggcagatt tacaccgctg attaaataaa aattgtcttt
     2701 accgatttgt tcggagaaag cgttagacac gcttcctccc agtaaatcta ccgtcacaat
     2761 taattcgtca tcaggcgcta agtcggaaaa tagctgctct atttcctggt taatggagta
     2821 gtcactatct atatacgcag ttattgctaa aagtgatttt tgaggaccaa taataaattc
     2881 taaagtatcc tttaagcctt tagccatcat ggaatgcgtc gcaattacaa ttttcctcat
     2941 agctcttacc ttaatattcc acagtccgat aataacgacg aatatctagt gaatgatccc
     3001 gcaccttttc aaaatgagca ctcactcgtt ctaaggccgt tgccataacc attggcgcta
     3061 catactgacg catagcgctt ggtacacctg ctaattcgaa atccgcagtg tcaaaaaccg
     3121 tcacaacatc gctatattta tttacaaaac gctctacgcg atcgactagc ggacgcgttt
     3181 gatcttcacc ttttaataac acaaaactca tagatttatc agtcatttca atagtcccat
     3241 ggaaaaattc ggctgcatgg atcgacttgg tggcaatcca ttgcatttct tccagtacgc
     3301 acatagcata ttcataggca gccccataag tattcccggc tccaacaaac atatgaaatt
     3361 ccgtattttt atgtttttct gcaaattcaa gtgctttctg atcattttgt tgccgaacac
     3421 ttagtaatac ttccggcatt ttttgtaatg tttgaataaa ttcatcatat tcaggaaact
     3481 caccgttatt tttcatcagg cgtgcaccca aagcaaaaaa ctgtaactga agttcttcta
     3541 ctaatgtatc gttgtcagca tcattttcaa tcgcataatc cacgatttcc ccgagtggtg
     3601 tagcagcatt tcctacaaac gcaatcgtcg ttgcaccttg actattggca tacttaacaa
     3661 catcaacggt ttcttgagtc gtaccggtta atgaagatgt gattaacact gtatctttat
     3721 taatcttttt cggctgtgaa caaaccacct ctgcggctat ttcatgataa taatcaagct
     3781 ctgaatgagt gcttaacaga taagctagcg ggctgattgt ggcatatgtt ccaccagagc
     3841 ctaccaaata aagattttta aatcccttct cactgattgc atctgccacc tgattgattg
     3901 tctcgatatt ttttactgca ccatttaata ctgatagaaa acgttcttcg ctaaaaccta
     3961 acatagttaa aaacctccaa gtcaataatt tgaaaaatat atttgcaaat gccggcacat
     4021 taacaaacca cttaaccttt tttatatacc acctgttaac gtttttaatg taccacttat
     4081 agaaaacgct gtcaatagtt tttaaccact tttattttta tttgtaccaa aatccttttt
     4141 tcatgtataa taaacaaaaa tagactctgg agtgtaacgt atgaatactg aagaactaac
     4201 gaacaaatta atacaagaca ttgaacaaga aaaatataaa tacgcgcaag ctattccttc
     4261 agaacgtgat tttgttcaaa tttatgaaat gaatcgttca gccgtaaaaa aagccattca
     4321 acaattagta aataaagagt acctactaaa aatacatggc aaaggaactt ttgttaaaaa
     4381 aaatatccat gaacatttgc aaatcaaatt tcatggtttg tctgaattgt tggaacggga
     4441 agggattcag cctagcagct taattttagc ttcagaaata aaaccggctg gacacattgt
     4501 cgggaaagct ttacagctgg aaccaactga agaagtattt catttggttc gtttaagaag
     4561 cggcgacggt gagccaattt ctattgaaga cacttatatt ccattaaatc tcatacctga
     4621 tcttcaagct tttgatttcc aaattttttc attttattcc acattggctg cttatcatta
     4681 tcaaattgaa aatatctatc agacaatttc cagctcaaaa gtgagcaata aagaagcccg
     4741 tttgttaaaa accagcttaa atcgtccggt aatcagcctt gctatcactg cggttacgac
     4801 aaatgaacaa gcggttgaat acactgaagt agtggttttg cctgaattcg ccgccatcta
     4861 tactaaaaat gttcatcaag gaaaggagat gaaaatctat gcccaaattg attaatctgt
     4921 tggcttatga cctgattgac aaaattattg cttatattca tgaacatcat ttaacccccg
     4981 gaacaaaact gccttcggaa cgggacctat cagaaatgtt taatgttaat cgaactacct
     5041 taagaggcgc gttacaaaaa ctggtggaca acggcacctt acgggcgcaa accaatagcg
     5101 gctattattt aactgcgcca aaattcgaaa aaaatgccca tcatttcttt acaccgatag
     5161 aagattctct gcttagtaag cagcggctac agctaaaggc aacggctctt tcaccgaaaa
     5221 aagcagcagc tctttgtccg gaactgaatg atttcaaaca gcagcaacca caagcttatt
     5281 tagaatatat tgatgcagaa gcaattagct tgatgttatt aatccctatg gaaacattaa
     5341 agaaaagtaa tttctcggac aatcctttcc aagattttaa aagtttggca tatacgcgtt
     5401 ctcaagaaat ttctttaata actgacaatg acgttgcggc ggacttgctg ggagttgaca
     5461 aagaagaggt tctgttattt gtacgagagg aaataagagc cggtaaggag ttggcggcga
     5521 ttgctttagg ttatactatt agtcgccgct gcaacataca ggttaatgtt gctacgacct
     5581 tgcaatccgg ttctgttgca aagttttaaa tctactatca gataaggtag aataatagaa
     5641 aaagatagca ggaggaatga cgatgagtca ttttaaagga aagcaatttc a
//
LOCUS       DACDLI010000113         5643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDLI010000113 DACDLI010000000
VERSION     DACDLI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.113"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..117
                     /locus_tag="IV353_002562"
     CDS             <1..117
                     /locus_tag="IV353_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9010734.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            159..605
                     /locus_tag="IV353_002563"
     CDS             159..605
                     /locus_tag="IV353_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010735.1"
                     /translation="MQQNRIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKM
                     SLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVS
                     YKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            619..1308
                     /locus_tag="IV353_002564"
     CDS             619..1308
                     /locus_tag="IV353_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010736.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1433..3784
                     /locus_tag="IV353_002565"
     CDS             1433..3784
                     /locus_tag="IV353_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9010737.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3864..4259
                     /locus_tag="IV353_002566"
     CDS             3864..4259
                     /locus_tag="IV353_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010738.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4584..5201
                     /locus_tag="IV353_002567"
     CDS             4584..5201
                     /locus_tag="IV353_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9010739.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5242..5541
                     /locus_tag="IV353_002568"
     CDS             5242..5541
                     /locus_tag="IV353_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010740.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
BASE COUNT         1969 a          757 c         1149 g         1768 t
ORIGIN      
        1 acgattaaag gcatggaagc catccgagga ttatataaga aaacccgaaa agaaggcact
       61 ctcttcgggt tttcggtctg tactgaaatc aaggtattat tgggaatccc agcttaaatc
      121 atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac cgtatttttt
      181 cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct gttaattcaa
      241 acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg agtttgacca
      301 aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat ttatccgggg
      361 aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt gtcttgtctg
      421 ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa caaaatcaca
      481 gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat ctaaaagagc
      541 ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca gtggaattag
      601 agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct acttatgatg
      661 ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga tccagagcag
      721 tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc attgccagaa
      781 ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga tataattcaa
      841 ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc aaaaaaaatt
      901 gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg aggagcattt
      961 ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg aagctataaa
     1021 tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa accagctgat
     1081 ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt aggaaatgta
     1141 aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta tgctcgtgaa
     1201 ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg tatttattac
     1261 acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata aaacagcaca
     1321 aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta aaatataaaa
     1381 tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt ttatggatga
     1441 gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta cagggtttga
     1501 taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta ttgaaggact
     1561 acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca ctgaaaaaat
     1621 gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata ttgtatttct
     1681 tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg gggtatatac
     1741 tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta atggaagcgc
     1801 tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga gtgcttttgt
     1861 tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa atgtaaatta
     1921 tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt caaatcaagc
     1981 attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa cagggtctta
     2041 tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg gaaactatgt
     2101 aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta cattcgatgg
     2161 tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta tggtattgcc
     2221 tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta gcgctggaga
     2281 tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat ctcaaatcca
     2341 aacattggtt gataatgata taaaaatagt tggtagatat cttactggta ctgttggaat
     2401 tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata ttttttctaa
     2461 aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt attttactta
     2521 tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc taaatatacc
     2581 attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag aaattcttgc
     2641 atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg ggtatcaaac
     2701 gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt atgcttcgtt
     2761 tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc ctatgcctag
     2821 acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg gagctgttgg
     2881 tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat caaatgatac
     2941 taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga tggtacgtgc
     3001 gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt tgacatttta
     3061 tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg taacagagca
     3121 tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg gcctgtataa
     3181 ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa ttgtgacgtt
     3241 acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact ttgcaggttg
     3301 gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac aaatggagca
     3361 aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag aagatttata
     3421 ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg attcaacagc
     3481 tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat ttaatcaatt
     3541 tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata gtttattaga
     3601 aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca ttgataaaat
     3661 agttaataat tcacttggga cagattttac ttatcttgat tttattggag atgaggggaa
     3721 agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa gtaaacttaa
     3781 ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat acgataaaat
     3841 aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg aataatctga
     3901 ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt atagttttgg
     3961 gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat actcctgaag
     4021 aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat ccaagagata
     4081 attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt gaagttacag
     4141 ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta aaggcaaaag
     4201 tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta atagaataat
     4261 agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa ataaaatata
     4321 ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag accgttaagg
     4381 cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg taaacttact
     4441 gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta tggcataata
     4501 accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa acaattttag
     4561 tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc agtagttatg
     4621 atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct gaagaatata
     4681 tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa ttaatgaaac
     4741 ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta ggcagaaata
     4801 aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt gatatttctg
     4861 tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg ttagagacgc
     4921 ttatctctga cttaattctg caattactca gctatatggc cgaagacgag cggaaacgaa
     4981 ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta aaattcggca
     5041 gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg aaagcaggaa
     5101 agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc ttttatcgac
     5161 gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa cagctattag
     5221 acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt tactggaacg
     5281 ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa cggccattgt
     5341 caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc taagtcgttg
     5401 tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat tgcgttaggt
     5461 catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca acagttgaaa
     5521 gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat ttttatgaag
     5581 cgaaaatctt atgatcgttg gggcggttct gttgcaaagt tttaaataaa gaataaaatc
     5641 cct
//
LOCUS       DACDLI010000114         5521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDLI010000114 DACDLI010000000
VERSION     DACDLI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.114"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..546
                     /locus_tag="IV353_002569"
     CDS             <1..546
                     /locus_tag="IV353_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9010741.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="IV353_002570"
     CDS             complement(716..1678)
                     /locus_tag="IV353_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9010742.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="IV353_002571"
     CDS             complement(1680..3119)
                     /locus_tag="IV353_002571"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9010743.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="IV353_002572"
     CDS             3304..5250
                     /locus_tag="IV353_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9010744.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1806 a          923 c          907 g         1885 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcattta atttaaaaaa ataaaatata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaagca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atggaatttt
     5281 ctttccaaaa tcaaataata tagattaatc aaatatttga gaataacatt ttatttaatc
     5341 ttttcatata tcaattgtat gaaaaattat tagaatttta aaaaatgata aaaattcaaa
     5401 aagaaaaaat agtagttatt tacttgtcat gttactaaat aaaaaaataa tttaaatatg
     5461 atatatagtc atttatttag tgatttcatt ctaaaataaa gggagaaatg tctatgctat
     5521 a
//
LOCUS       DACDLI010000115         5463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDLI010000115 DACDLI010000000
VERSION     DACDLI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.115"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..933
                     /locus_tag="IV353_002573"
     CDS             <1..933
                     /locus_tag="IV353_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9010745.1"
                     /translation="RWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLA
                     IDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHI
                     ESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAM
                     LIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVT
                     QNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCL
                     ISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            936..3119
                     /locus_tag="IV353_002574"
     CDS             936..3119
                     /locus_tag="IV353_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9010746.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3116..4117
                     /locus_tag="IV353_002575"
     CDS             3116..4117
                     /locus_tag="IV353_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9010747.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4132..5046
                     /locus_tag="IV353_002576"
     CDS             4132..5046
                     /locus_tag="IV353_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9010748.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5291..5407
                     /locus_tag="IV353_002577"
     CDS             5291..5407
                     /locus_tag="IV353_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9010749.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1727 a         1048 c         1154 g         1534 t
ORIGIN      
        1 agatggaaag aaaccttgcc agagatttcc catgaaatca atatcgtcaa tctgacttct
       61 gatgagaaaa acaaaggctt acttgaccct tatgtaatta tgaaaaatac caaagattct
      121 gaatcactgg ctattgatat tttgacattc cttacgggga tttcctctcg tgatggggaa
      181 cgcttcccaa tccttagaaa agccattcgt gcagtaacca atagtgaagt gcgagggttg
      241 atgaaagtga ttgaagaatt acgggttgag aatacgccac taagtaccag catagccgac
      301 catatcgaaa gttttacaga ctatgacttt gcccatctgc tttttagtga tggttatgta
      361 gagcagtcta tcagccttga aaaacaactg aacattatac aggttgccga cttggtactt
      421 cctgacaagg aaacttcctt tgaggaatat accacaatgg agttactatc ggtagcaatg
      481 ctgattgtca ttagtacctt tgcgttggac tttatccata cagaccgaag tattttcaag
      541 attgtagact tagacgaagc atggagcttc ttacaggtgg cacaagggaa aacactatct
      601 atgaagctgg ttcgtgctgg tcgtgctatg aacgctgggg tatatttcgt gacccaaaat
      661 acagatgacc tcttagatga aaaactgaaa aataacctcg gcttaaaatt tgcctttcgt
      721 tccactgacc ttaacgagat taaaaaaacc cttgcctttt ttggtgtaga cccagaggac
      781 gaaaacaatc agaagcgatt gcgtgatttg gaaaacgggc aatgccttat cagtgattta
      841 tatggtcgtg tcggtgtgat acagttccac cctgtatttg aagaactgct ccatgccttt
      901 gataccagac cacctgtgcg aaaagaggtg taaatgtgaa accatcaata ttaaacagaa
      961 taaaatcaaa ctggacgctg aaacgtctag gtaaagtggc aatgacagtg gctttcacac
     1021 ttgtgattgc catttttctt ttagccatgc tgggaacggt ggttcaagct gcgggcttgg
     1081 tagatgatac ggtcaatgtg gcaaatgaat acagccgata cccacttgaa aactatcaac
     1141 tggattttta tgtggataat agctggggct ggctaccgtg gaactggtcg gacggaattg
     1201 gaaaacaagt catgtatgga ctatatgcca ttaccaattt tatttggaca atcagtcttt
     1261 atgtttccaa tgcgacgggt tacttagtac aggaagccta ttccttagac ttcatttccg
     1321 ctacagcaga ttccattggc aagaatatgc agacccttgc aggggttacg cctagtggac
     1381 tatcaacaga gggtttctat gttggattcc ttttactctt gattttggtt cttggggttt
     1441 atgttgccta tacaggactg ataaagagag aaaccacaaa ggcaattcat gccctcatga
     1501 attttgtgct ggtttttatc ctatcggctt cctttattgc ctacgctccc gactacatta
     1561 aaaaaatcaa tgacttttca tcagacatca gcaacgctag tttgtcactg ggtacaaaaa
     1621 ttgtcatgcc ccattcagat agtcaaggca aggacagtgt ggacttgata cgtgacagcc
     1681 tattttccat acaggttcag caaccgtggc tactgcttca atataacagt tcagacattg
     1741 aaagtattgg tattgaccgt gtggaaagcc tgctctcaac cagtccagat tccaacaatg
     1801 gggaagacag agaaaaaatt gttgcggaag aaattgaaga tagaagcaat accaatatga
     1861 cgattacaaa gacgattaac cgtttaggta cagtcttctt cctatttgtc ttcaatattg
     1921 ggatttccat atttgtattc ctattaacag gaatcatgat tttctcgcag gtacttttta
     1981 tcatctatgc tatgtttctg cctgtgagct ttattttaag catgattcca tcatttgatg
     2041 gtatgtcaaa acgagccata acaaagctct ttaataccat tttgacacga gctggaatca
     2101 cattgattat tacgacagca tttagtattt caaccatgct ctatacctta tcggctggtt
     2161 atccgttctt tttgattgct tttctacaga ttgtgacctt tgcaggaatc tactttaagc
     2221 tgggcgattt aatgagtatg ttttctctac agagtaacga ttctcaaagt gtgggaagtc
     2281 gtatgatgag aaaacctcgt atgcttatgc acgctcacat gcaccgtcta cagcggaaac
     2341 ttggacgctc cgtgactgct ttaggggctg ggtctgccat tgccagtgct acgggtaaaa
     2401 aaggacagtc gggttcggga agttctgcga gtacacaagc agatcactct cgaccaaacg
     2461 ggcaggaaaa atcaacacta ggaaagcgta tcggtcaaac catcggtaca gtagctgata
     2521 ccaaagacag aatggtagat actgctggta atttgaaaga acaggttaag gatttgccga
     2581 ccaatgcaag atatgcagta tatcaaggaa aatctaaagt gacggataat gtccatgatt
     2641 taaccagaag tatttctcaa accaaagcgg ataaagccag tggacgcaag aaacagcagg
     2701 aacaaagacg gaaaaccatt gcggagcgtc gctctgaaat ggaacaggtc aaacagaaaa
     2761 aacagccagc ttcttccgtt catgaaagac cagccactaa gcaagaacaa tctcatgatg
     2821 gacagacttc aaggcaaaca actgtacagg cttcctatag ggaatctcaa caagccaaac
     2881 aagagcgtcc aacagttaag tccgattctt caagtttaaa aacggaacgt caaagtaata
     2941 cagttcaaga aagaacggtt caaaagccag taacttcaac tgcaccaaca gatagagctt
     3001 cacaacgttc aatcacaaaa gaacgtcagt ctactgttca aagagtacca ctacaaaata
     3061 caaaaagtag accaccaatc aaaaccgcca ccattaagaa aggcagtaag aaaccatgaa
     3121 gctgaaaact ttagtgattg gtggttcggg attattcttg atggtcttct cactgcttct
     3181 gtttgtcgcc attttatttt cagatgaaca agatggcggt ttttccaaca ttcactatgg
     3241 cggtgtggat gtttccgcag aagtgctggc tcataagcct atggtagaaa aagttgccaa
     3301 agaatatgga attgaagaat acgtcaatat acttcttgcg attatacagg tggaatcggg
     3361 tggtacagca gaagatgtta tgcagtcctc ggaatccctc ggtcttccac ctaattcatt
     3421 gagtacagaa gaatccatta agcaaggtgt gaagtatttc agtgagttat tagccagtag
     3481 cgaaaggctt ggtgtagatt tagaatcggt tatccagtcc tacaattatg gtggtggttt
     3541 cttaggatat gtggctaatc gtgggaataa atataccttt gaactggctc aaagtttctc
     3601 aaaagagtat tcgggtgacg aaaaagtgtc ttaccccaat cccatagcca tacctatcaa
     3661 tgggggctgg cgatacaatt acgggaatat gttttatgtg caactggtaa cgcagtatct
     3721 tgtcacaaca gagtttgatg atgatacagt acaagccatc atggacgaag ctctgaaatt
     3781 tgaaggctgg aagtatgtct atggcggagc ttccccgact acctcttttg attgtagcgg
     3841 actgacacaa tggacgtatg gaaaagctgg aatcaactta ccacgaacag cacaacagca
     3901 atatgatgta acccggcata tcccattatt ggaagcaaaa gccggcgatt tggttttctt
     3961 tcattctacc tataacgctg gctcttatat tactcatatc gggatttatt taggcgataa
     4021 ccgtatgttt catgcaggcg accctatcgg ttatgccgac ttaacaagct cttactggca
     4081 acagcattta gtgggagcag gacgaatcaa acaataagaa aggacgattc aatgatgaaa
     4141 ttcggaaaaa atcagaatac agaaaaacag acaccaaagg aaaagaaacc tcgtgtctat
     4201 aaggtcaatc ctcgtaaaaa ggttgtgatt gtcctatggg tacttttagg acttagtttc
     4261 agctttgcga tattcaagca ctttacagcc attgataccc ataccattca tgaaaccacc
     4321 atcatagaaa aggaatatgt cgatactcat catgtggaaa attttgtaga gaactttgcg
     4381 aaagtctact attcatggga actaaacgac aagtccattg ataatcgcat agaaaacctg
     4441 aaagcatatc tgacagagga acttcaagct ctgaatgtcg atacagttcg taaagatatt
     4501 cctgtatcgt cttctgtaaa aggatttcag atatggacgg tagaagcaga tggcgacaat
     4561 caatttgatg taacctacag tgtagaccaa ctcattacag agggggaaaa tacaaagacc
     4621 gtccactctg cttatatagt gagtgtctat gtagacagta ctggaaatat ggtcctcatt
     4681 aagaatccga ccattactag cataccaaag aaatcagact ataaaccaaa agctattgaa
     4741 agtgagggta cggttgattc cattacaacc aatgaaatca atgagttttt aacgacgttc
     4801 ttcaagctct atcctacagc gacagccagt gaactttcct actatgtgaa tgacgggata
     4861 ttaaaaccaa tcggaaaaga gtacatcttt caagaactgg taaatcctat ttacaatcgt
     4921 aaggataatc aagtcacggt atcgctgaca gtggagtata tcgaccagca gaccaaagca
     4981 acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg gaagattata
     5041 gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc tctttgataa
     5101 aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt atctatttaa
     5161 aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagtggga
     5221 acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgcacccag tttaagaata
     5281 cctttatcat gtgattctaa agtatccgga gaatatctgt atgctttgta tgcctatggt
     5341 tatgcataaa aatcccagtg ataagagtat ttatcactgg gatttttatg cccttttggg
     5401 cttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga
     5461 tgc
//
LOCUS       DACDLI010000116         5447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDLI010000116 DACDLI010000000
VERSION     DACDLI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.116"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..488)
                     /locus_tag="IV353_002578"
     CDS             complement(<1..488)
                     /locus_tag="IV353_002578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010750.1"
                     /translation="MNIITRDELIYAYKDEVNLLEVSQYRSSNVITLNIDTVSEFIKL
                     AKELKINHVYYNYLYYSIDNYLIPLDTYDEFSDEINIEVKKHNEVVDSLDFSKPKQLT
                     LLIVLNGTIMEITLNHFWIKESGIPDNETKHSEIQDKFFKDFKLQQEEISRNEHDDKN
                     RL"
     gene            complement(593..1156)
                     /locus_tag="IV353_002579"
     CDS             complement(593..1156)
                     /locus_tag="IV353_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017550078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9010751.1"
                     /translation="MSGYIYGYARVSTRSQELNRQIDLLNEQNCNEILTEKMTGTKAD
                     RPQLNRLKDKVRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHEVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQRDIERAVKLYDSQ
                     IYSIKEITEMTGISKATLYRYLKTNNF"
     gene            1532..1762
                     /locus_tag="IV353_002580"
     CDS             1532..1762
                     /locus_tag="IV353_002580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010752.1"
                     /translation="MLKNLFFKNKKQLTILEKIFYFFVGNKNRMQNKSYYVRERAGAK
                     WRYRKFKDPWIAKNRNTLFKRVFGFFEDIFRG"
     gene            complement(1797..1946)
                     /locus_tag="IV353_002581"
     CDS             complement(1797..1946)
                     /locus_tag="IV353_002581"
                     /inference="COORDINATES: protein motif:HMM:NF025625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9010753.1"
                     /translation="MIEKNGVLRTILDISYNSPSRVAELISGNSTNGWSFVEGLSELR
                     TMDQE"
     gene            2021..2284
                     /locus_tag="IV353_002582"
     CDS             2021..2284
                     /locus_tag="IV353_002582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010754.1"
                     /translation="MILQVYNEQQGLTGGSSESSSSPESSEPESAAPSTPAGGDVSSV
                     DTNGNGNVTIKEAKAAGYSMPITSDHWLYQYMNDRDNDGMVGE"
     gene            complement(2384..2815)
                     /locus_tag="IV353_002583"
     CDS             complement(2384..2815)
                     /locus_tag="IV353_002583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010755.1"
                     /translation="MTIYLEENERIFEGKELYLIRNFSRKGIGFFEESGFYPDFIMWY
                     FNGSQEYIIFIEPHGMLRENLDGNKVQLYKEIKKVENEISLSEGTRPVLESFILSPTP
                     FATLNNPINNPSNYSKGDLNRQHVIFMEDENYIEQLFESLI"
     gene            complement(2964..3479)
                     /locus_tag="IV353_002584"
     CDS             complement(2964..3479)
                     /locus_tag="IV353_002584"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010290771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HAP9010756.1"
                     /translation="MVPLTSEKIFGLAPIIDDQAKILILGSMPGKESLKKEQYYANKR
                     NQMWKIMENTLKLQLPEEYPKRINVLRKHHIALWDVIHSCKRVGSLDSAITEDVPNDF
                     GSFFKEYSSLKIVGFNGNKAYQTFKSKIGFQDFPEIQFIKLPSTSPIPGKNVKSFEEK
                     VVEWSKVVNSL"
     gene            complement(3788..4150)
                     /locus_tag="IV353_002585"
     CDS             complement(3788..4150)
                     /locus_tag="IV353_002585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010757.1"
                     /translation="MADNLLNKNKGKRNLLERKEEVKPQQSFNRSSLFTVNESQQTNG
                     DQKKDVPRKKERGTTTTIRCATDTSHRLNAIVTSFNLDSVNELIEILLDNYEASLTTD
                     ERREIKTLLEVYQRKSKK"
     gene            complement(4192..5028)
                     /locus_tag="IV353_002586"
     CDS             complement(4192..5028)
                     /locus_tag="IV353_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9010758.1"
                     /translation="MAKVITFGNFKGGTGKTTNSAMMAYTLSKLGYKTLLADLDPQAN
                     ATSLYLHTKQRITNNIVTFDKTLMSAIADEDLSDIIIEIKENLFLLPSFADFTSYPLF
                     LEKKFPDSQFNRVTFLNELMTKIKDDYDFILVDVPPTLSTYTDSALLASDYTIIVLQT
                     QERSLVGAEAYVGYLQELIDNYNADFDILGVLPVLLKNNSKVDEATLNTAKEKFGEEN
                     LFENVVKNMERLKRYDIIGIVDPDLDTKYDIHDKRVMGLYKRVTEEMLKRLEEIESRN
                     KQ"
BASE COUNT         1713 a          970 c          834 g         1930 t
ORIGIN      
        1 tttaatctat ttttatcgtc gtgttcattt ctagatattt cttcctgttg aagtttaaaa
       61 tccttgaaaa atttgtcttg tatctcagaa tgcttagttt cattatctgg tattccgctt
      121 tcctttatcc aaaaatgatt taaagtaatt tccattatag taccattcag tacaattagt
      181 aaggttagtt gttttggttt agaaaaatca agactatcta ccacttcatt atgcttttta
      241 acctctatat tgatttcatc agaaaattca tcatatgtat ctaagggtat gaggtaatta
      301 tcaatagaat agtatagata attataatat acatgattaa tctttaattc ttttgctaac
      361 tttatgaact ccgataccgt atcaatattt aaagtaatta catttgagga tcgatattga
      421 ctcacttcca ataaattgac ctcatcttta tatgcataaa ttaattcatc tctagttata
      481 atattcatta actccacttc ctacccgatt attatataat ccaaaaatat cacttcaatc
      541 aattattaat ttttcagaat taaagatgaa taatttcatt tcatgataag atttaaaaat
      601 tatttgtttt taaatagcga tagagtgtag ctttactgat gccagtcatt tctgtaattt
      661 cttttatgct gtatatttga ctgtcataca gtttcaccgc tctctcaata tctcgttgat
      721 taacgcgtgg gcgtcctcct tttcggcctc tagcacgcgc actttttaac ccttctttgg
      781 tacgctccac aatcaaatca cgttcaaact gactaaacgc ttggaagacg gtcatcatta
      841 agcgtccgtg aggagtgcta gtatcgaagt tttctttcaa actcaccagc ttcacttcat
      901 gttcttcaaa gtaggtaacg agatcaatta aatctttggt actccgacct agacgagaga
      961 aactttccac gataaccgtg tctccaggac gcaccttatc ttttagtcga ttcaactgag
     1021 gacgatcggc tttggttccg gtcatttttt ccgttaggat ctcattgcaa ttttgttcat
     1081 tgagtaggtc tatttgtcga ttcaactctt gcgagcgtgt actcactctg gcatagccat
     1141 atatatatcc actcataaaa aatcatcctt tgtttttgat attattgtat cataaacggg
     1201 agttaaaggt gcatacataa agagagaagt tttgagacta aaaaggacta ctttttaggt
     1261 gaaaaatccg tcctttaaaa tcgtctcata atcgattgtt tttgagactt caggtttaat
     1321 tttaactgac ttaatttttt gagttattat ctaaaagctt taagtttaag gagttgatta
     1381 ttttctggaa acaataaacc aaaatttgtt taacctgttg tgccatataa tttttactta
     1441 ggcacacttt aacgtatttt tagtttaggg gtatgctaat atagtagtta gatacttaca
     1501 cagtattaag gctgagccaa gggggaaagg aatgcttaaa aacttatttt ttaagaataa
     1561 gaaacagcta acaattttag aaaaaatatt ctattttttt gtaggaaata aaaaccgcat
     1621 gcaaaataag tcctattatg ttcgagaacg tgcaggggca aagtggcgat atcgaaaatt
     1681 taaagatcca tggatagcaa aaaatagaaa caccctattc aaaagagtct ttggtttttt
     1741 tgaggatata tttagaggat aaatttccaa ataaaaaaga ggcccatgcc tcttttttat
     1801 tcctgatcca tagtccgcaa ttcacttaaa ccttcaacaa atgaccatcc atttgttgag
     1861 tttcctgata ttaattcagc aactctcgaa ggactattat aggaaatatc aagtattgtt
     1921 ctcaaaactc catttttttc gatcactttt ccatcttcaa tgtaactgtc aatcacatcg
     1981 ttatagcgaa taaagaaatt accagtcttc cattgcgcac ttgattctac aggtctacaa
     2041 tgaacagcaa ggtttaacag gaggaagtag tgaatcttca tcttctcctg aatcctcaga
     2101 gccagaatct gccgcaccaa gtactcctgc tggaggagat gtttcctctg tagataccaa
     2161 tggaaatggg aatgtaacca ttaaagaagc aaaagcagct ggctattcta tgcctattac
     2221 tagtgatcat tggttatatc aatatatgaa tgatagagat aatgacggta tggttggaga
     2281 ataaaccaaa ataatgaaaa atgaaagacc agattaatga actgccccct gtcaagtaga
     2341 cagggggcag ttcattcgga aatttagtct cttttatctg tttttatatt agtgattcaa
     2401 ataactgctc aatataattt tcgtcttcca taaatatgac atgttgtctg tttaagtcac
     2461 ctttagaata attagatgga ttattaattg ggttatttaa tgtagcaaat ggtgtgggag
     2521 atagaataaa ggactctaga actggacggg taccttctga caatgaaatt tcattttcta
     2581 cctttttaat ttctttgtat aactggacct tattaccatc taaattctct cgcaacatac
     2641 cgtgaggctc aataaatatt atatattcct gactgccatt gaaataccac atgataaaat
     2701 cgggataaaa cccagattct tcaaaaaatc caattccctt tcttgagaag ttacgaatta
     2761 agtaaagttc tttaccctca aaaattcgtt cattttcctc taagtagatt gtcaagtctt
     2821 taataaattt ccattcacta tcaactaacg cggtagggac tacttgaata tcggttagtc
     2881 cttgttctac atgtatcaac gggttatata aagatttatg tttcttttag taggctctga
     2941 ttttcaaatc aatacctaaa tgtttataag ctatttacta ccttgctcca ctctacaact
     3001 ttttcttcaa acgattttac gtttttccct ggaattgggc tagtagaagg taacttaata
     3061 aactgaatct ctggaaaatc ctggaaaccg atttttgatt taaatgtttg atatgctttg
     3121 ttcccattga acccaacaat ttttaaggat gagtattcct taaaaaaaga cccaaagtca
     3181 tttggcacat cttctgtgat ggcagaatct aaactcccta ctctcttaca actatgaatc
     3241 acgtcccata aagcaatgtg gtgttttctt agaacattaa ttcgttttgg atattcttca
     3301 gggagttgta atttgagggt gttctccata attttccaca tttgattcct tttgttagca
     3361 taatattgct cttttttgag agattctttt ccaggcatgg atcctaaaat caaaatttta
     3421 gcttgatcgt cgataatagg agctaaacca aatatttttt ctgaggttaa tggaaccata
     3481 tttttatccc ctttgtagtt cagtcacata agagttaatt ttttaaaacg ttatggtatc
     3541 taatccttgt agtatactag aatgtttttt ttagcttttt ttggtgtcta ttgacgactt
     3601 tatcttcgta tatttgaact aaatcctcaa aacgtttggt gtcactattg tttaaatttt
     3661 caacgtataa gtttacaaaa gtccttccca tttttttgac tacatcattg taattgccat
     3721 tatatagcga aatttacacg attaaagaga cacgtctaca tgtagacgtg tctacaaatc
     3781 tacaaatcta ctttttagat tttcgctggt aaacctctag taaagtcttt atttctcttc
     3841 gttcatccgt cgttaaagaa gcctcataat tatctaggag gatttctatc agttcattaa
     3901 cactatctaa attgaaagaa gtaacgattg cgttcagacg atgtgaagta tccgttgcac
     3961 aacgaatagt agttgtcgtg cccctttctt tttttcgagg gacgtctttt ttttggtctc
     4021 cattagtttg ctgagattcg tttactgtaa ataaactgga tcgattaaaa gattgttggg
     4081 gtttcacttc ttcttttcgc tcaagaagat tccgtttccc tttattttta tttaataaat
     4141 tatctgccat actatcaccc tcttctttct tgtatatggt atgaataatg tctattgttt
     4201 gtttcgagat tctatctctt ctaatcgttt caacatctct tcagtgacac gcttataaag
     4261 ccccattact ctcttatcgt gaatatcata tttagtgtct aaatcaggat caactatccc
     4321 aataatgtca tatcgtttta atcgttccat atttttaact acattttcga agagattttc
     4381 ttcaccaaat ttttctttcg cagtattgag cgtagcttcg tcgacctttg aattgttttt
     4441 tagtaatacg ggtagtacac ctaaaatatc aaaatccgca ttatagttat caatgagttc
     4501 ttgcaagtac cctacataag cttcggcccc taccaaagaa cgttcttgcg tctgtaggac
     4561 aataatggtg tagtcggaag ctaacaaagc cgagtccgta taagtactta aagtaggtgg
     4621 aacatcgacc aaaataaaat cgtagtcatc cttgatctta gtcattaatt catttaaaaa
     4681 agtcacccta ttaaattgac tgtctggaaa tttcttttct aaaaataagg gataagaggt
     4741 aaaatctgca aagctaggca ataaaaaaag attttctttg atttcaataa taatgtccga
     4801 taaatcttca tctgctatgg cagacattag cgtcttgtca aaagtaacaa tattattcgt
     4861 aattcgctgt ttcgtatgta aatacaaaga tgttgcattc gcttgaggat ctaaatccgc
     4921 taataatgtc ttatagccca gtttagataa tgtgtaagcc atcatggcac tattagttgt
     4981 tttacctgtt ccacccttaa aatttccgaa tgttataact ttagccattg taaccctctt
     5041 ttcttagttg tctacaaatc tacatttgat attataacgt aaatatacca tgctaaatca
     5101 atatgtcaaa cgttatcaag aaaaaccgtc tacaaatata catgtagacg tatctacaaa
     5161 tctacacgtc tacatgtaga cgcatataca ttaaaaatct tgataacaaa aggatatcaa
     5221 gcatcatgta tatgcatcta caaatctaca aatctacatg tagacgtgta tacaaatcta
     5281 caaatctaca tgtagacgca tctacagaaa aacgagattt tgcctacaaa ttaaggtttt
     5341 acaaaaaaaa aatagtgtag tacatttgat ttaattcaaa gcaaaactaa aattgaatac
     5401 aaaaaaagcc ccgtagacgc caatctacga gacacgcaaa acttatc
//
LOCUS       DACDLI010000117         5403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDLI010000117 DACDLI010000000
VERSION     DACDLI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.117"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..239)
                     /locus_tag="IV353_002587"
     CDS             complement(<1..239)
                     /locus_tag="IV353_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010759.1"
                     /translation="MSENNYKKIGYNSLLNMSYEEAIHYLLNKYGEVSDDYFKEKSYA
                     RFLRGEIKTITKGKYSKTSEGLYCHHIYENKYENIS"
     gene            complement(591..1676)
                     /gene="xerS"
                     /locus_tag="IV353_002588"
     CDS             complement(591..1676)
                     /gene="xerS"
                     /locus_tag="IV353_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9010760.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVTTITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKNTLSKRALIYYHRDVERDLAINALILGSGLRLSE
                     VVNINLDDLSLDKNKVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2009..2242
                     /locus_tag="IV353_002589"
     CDS             2009..2242
                     /locus_tag="IV353_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9010761.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDVAISQALTEYDTTFRELVDK"
     gene            2239..2646
                     /locus_tag="IV353_002590"
     CDS             2239..2646
                     /locus_tag="IV353_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9010762.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLVVYIATYDKDFDQLKYEVINTIKTHTKPI"
     gene            complement(3783..4301)
                     /locus_tag="IV353_002591"
     CDS             complement(3783..4301)
                     /locus_tag="IV353_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9010763.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1775 a          878 c          819 g         1931 t
ORIGIN      
        1 gagatatttt catacttatt ttcatatata tgatgacagt ataagccttc tgatgtttta
       61 ctatattttc ctttagttat tgtttttatt tctcctcgta aaaatctagc atatgatttc
      121 tctttaaaat agtcatcaga aacctctccg tatttattta aaaggtaatg aatagcttct
      181 tcataagaca tatttaataa agaattgtaa cctatttttt tataattgtt ttctgacata
      241 attataaacc aagctcctta ctattttaag aatagagtat gagaaatata tcctatcact
      301 ttagagttgg aatttactta caaaaaacat ataatccctt aatattatac gcttatttaa
      361 ttttcaattt ctctatattg aaattcaatg aagttatcaa tattcctata aaccacctct
      421 acttgccagt aattataatt tattatgaat tactcaatag ttaatcatat ttattaacaa
      481 aagattattg gttacaacaa aaaaagaacc atgaatccat ttacgttaaa aggattcatg
      541 gtttcattca ttattctcac tttactattt tggctattta tatattatac tcataaagcg
      601 ttcaatgcat caatggtagc tgcattatct atatgagtat ataaagttgt tgtatttgta
      661 cttgaatgtc ccaattgttg ggcagtcaat accaaagaat ttgtctgttg gtagagtcta
      721 gtagccaatg tatgacgcag tttatgcgga ctgacttgta ctctgaatcc tttcgaatat
      781 tttgctacca tccgttcgat tgcaataccg gatatacgtt tggcttctcc ttggtaaatt
      841 gctagaaata atgctttttc gttttctgtc actttatatc gttctttccg aatagctaga
      901 taattagcta aatattccat tgcgaatgct gctatattga ccgcatctct cttatttcct
      961 tttctagtta ctaccacctt attcttatcc aaagatagat catccaagtt aatatttaca
     1021 acttcggaga gtctcaatcc agatcctaaa atcagtgcat tgatcgctag gtctctttct
     1081 acatctcgat ggtagtagat caaagctcgt ttgctcaaag tatttttata ttcattggca
     1141 atataatcta gataattttg tgtatcctca tctagaaata attttttctc aatggcattg
     1201 gcacgtgcag ctaacgtagt tttatctgtg atggttgtta cttggtccat aggattattg
     1261 tctaagtatg gttttcctag gggattatta gaacttttcg aaagatattt aaatagaacc
     1321 tttaatgctg tgatgtttct attgatagta gaagtagata gacctaagtt attattatct
     1381 ttgagcttag tttttgttgt ctcttttttt acattttctt ttgtgcgacg tttcaaatga
     1441 gatttgtaca agtttaggtc ataggcagtt aactgtctta tcgaggtgat agaagtatca
     1501 ggaacgatag actcactcag aagccattca taaaagagct tataattttt tagatattct
     1561 aaagtggttc gaggtgattg atctttatcc tcacgatctt gaacaaaatc ttttatataa
     1621 tcgggcatat gaagtatgat tgtatctatt ttttgatata tttctgtatt tttcatgtca
     1681 ttcatccttt caataagtag gggagaagag tatctctata attggactat accaattata
     1741 gaatgttttg tcaattggta tgctctaaaa tttattaatt tgtttcaaaa aaagcatata
     1801 aataggtata cgtttattaa tttaaaataa atggtataca taaataggga aggaaataga
     1861 agggagcaat ctaaattttt tgaattttct taattaatta tatagaaaga acaatctttc
     1921 tatagagtta gacatgagag gggctatgct tgatttaaaa tcaacaagcg tatatactgt
     1981 atatacaaag aaaaaggggg agaagactat ggcaattaaa gaaagaaaat ttagggaagt
     2041 ggggaactca gtagtattaa ctatttcaaa ggaatttctt aaagaaaatg ggttaaatcc
     2101 cggagatacg gtttttttag atgaagaaaa acttcgagcc gcaattacaa aaaaacaagc
     2161 cttatctgat gaaattgatg tagcaatatc tcaagcatta actgaatatg atactacttt
     2221 tagagagctt gtcgataaat gagtattcat tacctagaag ccaaagatat tataaaaatg
     2281 aatgtaatcc aaataaaaaa atactctcct aatgaacccc ttggtgtaaa ggatgcgaat
     2341 gctttagaaa tgtgtgtagg gcaacttcga gcctcagctt ttggagaaga ggtttatcct
     2401 tcgatttatg aaaaagcagc tatcttactt atacaattga taaagaaaca tccttttcat
     2461 aatgcaaata aacgtactgc tttcttagct acttttgtct ttttgaaaat caatggacag
     2521 cttttagcta ttgatcaaaa agatgctgta aatttagtgg tatatattgc cacatatgat
     2581 aaggattttg atcaactaaa atatgaagtg attaacacta taaagacaca tacgaaaccc
     2641 atttaaggag ttgctgtatg tgtctttata gtgttaatag ctgctatagc gaaataattg
     2701 aagtaaatta caaatagcta taatccaaat ccaagtatat agaaaacgta aattttctat
     2761 atagttggat taagataacg actatttatt ttatcatatt tttactatac cctatacttt
     2821 tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt tgtaagttag
     2881 attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa cttataaatt
     2941 gatggtattt agataacgcg aaagaataaa aataacagct gatagtaaag tttgttgacg
     3001 attgctactc aatcgaaagt gagttagcgc gtatgaactt gcttctgaaa acttatgaaa
     3061 tgaaagacct tttacatctt gcagaaatcg ttactgggat taaactaagc aataaaaact
     3121 aatgttcccc tcattctttg aacttctact atttgattta tttattctga taaatgacga
     3181 tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct tcagtatcag
     3241 taataggata ggcgataggc cacataccct ttgatggtta gaataaactg ctcaaaatct
     3301 gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata aaccataagc
     3361 gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga tataattacc
     3421 ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg aattgtcggt
     3481 actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg ctagtctcct
     3541 aagtcccttt gcagcggata tggcaccata atctcatttt atcgaagtga gtcatggttt
     3601 atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg cattctaggg
     3661 gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta tgtagtcgta
     3721 tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca gtaaacggga
     3781 atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc aaatggagca
     3841 actgtattta catgaataaa tttatatact tcccatacag ccgttttggt tgcctcgtct
     3901 gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc aatcatcatg
     3961 ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg tagatgtgca
     4021 taggtatttg taaaccactt atataactct cctaactgat tccatttaaa ttgatcagaa
     4081 ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat taaattcgtc
     4141 caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc aactctttta
     4201 tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata agtcctattt
     4261 atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc tccataaatt
     4321 cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat ccgttctaca
     4381 atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat aaaatatccc
     4441 aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac gaatatattt
     4501 cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat caaaatcatg
     4561 ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac tgttaagttt
     4621 agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg gtaaaactca
     4681 aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat
     4741 attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg aaacagctac
     4801 aaaaacgaaa tgaccaactc gaagaaaatt taattctaaa aaagcgattg ccatattcac
     4861 gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct ttttaggaaa
     4921 ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt tcaccagata
     4981 ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt caccgcgaat
     5041 ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa attaagggat
     5101 caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt taaacaatta
     5161 tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg actttcttat
     5221 tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt ttacgagtac
     5281 tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta cccccactta
     5341 gctaaacctg acggttctta acgcttgaag tgggagcttc ttgggaacag agcgtacttg
     5401 ata
//
LOCUS       DACDLI010000118         5363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDLI010000118 DACDLI010000000
VERSION     DACDLI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.118"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            161..493
                     /locus_tag="IV353_002592"
     CDS             161..493
                     /locus_tag="IV353_002592"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9010764.1"
                     /translation="MIAVSEATYKAFKTEFVSQYQFETMAHLELECFDYVNWWNHHRI
                     HSSLGYKTPIEVLCGGWRSEPLIMSKNRLPSVRAAYFIQSEHAAKKTDLRRLVVKGNW
                     IWCHKQTL"
     gene            complement(663..1058)
                     /locus_tag="IV353_002593"
     CDS             complement(663..1058)
                     /locus_tag="IV353_002593"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP9010765.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(1068..1916)
                     /locus_tag="IV353_002594"
     CDS             complement(1068..1916)
                     /locus_tag="IV353_002594"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP9010766.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1931..2758)
                     /gene="panB"
                     /locus_tag="IV353_002595"
     CDS             complement(1931..2758)
                     /gene="panB"
                     /locus_tag="IV353_002595"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP9010767.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2770..3630)
                     /locus_tag="IV353_002596"
     CDS             complement(2770..3630)
                     /locus_tag="IV353_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAP9010768.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3868..5037
                     /locus_tag="IV353_002597"
     CDS             3868..5037
                     /locus_tag="IV353_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9010769.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRDRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1721 a         1009 c          737 g         1896 t
ORIGIN      
        1 tacggaaaga agttctgcac cttataaaat ttgtcaaaaa aactgttgcc attccaactc
       61 ttccatacgg atcgtggaaa agaatttgat aatgaattga ttgatcttat cttagaaaca
      121 tttggcattc aacgctcctt gagtaagcct ggatgtccat atgattgccg tttcagaagc
      181 gacttacaaa gcttttaaga cggaatttgt gagtcaatat caatttgaaa cgatggcaca
      241 cttagaatta gaatgttttg attatgtcaa ttggtggaat catcatcgga tccatagttc
      301 tcttggttat aaaacaccaa ttgaggtact atgcggaggt tggcgtagcg aacccttgat
      361 aatgagcaag aatcggttac cttctgttcg agcggcttat tttattcaaa gtgagcacgc
      421 cgctaagaaa accgatttga ggaggctcgt tgtcaaggga aactggatat ggtgtcataa
      481 acagacctta taatttttgt ctagaaaagt gttgccattc catgtagaca tcatccataa
      541 aatggacttt gtctacagtc tgtatcttct ctttttgtgt ctactttatt gacatctatc
      601 caattagtaa tcaataaata tttcaataat agtctagcta tatataaata acattacaaa
      661 acctaaagaa ttatatctga taaccttcca tgtttttcat atgatgttac tctttcaact
      721 ttattattat catcgacaaa aacaacctta ggtctatgat ccttaacctc attttcattt
      781 aattcacaat atgccattaa aattatttta tctccagttg atacacatct agctgcagct
      841 ccattcaagc aaatcatccc tgttcctctc tctccagcta ttgtataagt ttcaaatcta
      901 tttccattat taacatcaac tatttgtact ttttcatatt catatattcc tgctgcttct
      961 aataaattca tatcaacagt aatacttcct acataatcta actcagcttg ttcaactgta
     1021 gctctatgta tttttccttt taacattgta actttcatta tagcccccta ttgtaattca
     1081 aatgtaaaat tatctattaa tcttgttttt cctataaata ctgctattgc aactaaaact
     1141 gatttttcaa tataatttac tctttgtaat gataaagaat caacaacttc tacataatca
     1201 atctttgcca aattataagt atttatattt ttacttatta tctcaattat ttttaagctg
     1261 tctctctctc cattatttaa agcttcctta gctaaagtta aagatttatt taaaatagta
     1321 gctgaagatc tttcctctaa acttaagtat gtgtttctag aacttttagc tagtccatcc
     1381 tcttccctta taattggaca ccctacaacg tctatatcaa tattaaggtc cctaaccatt
     1441 cttttaatta cagctaactg ttgtgcatcc ttttctccaa aataagctct atctgctggt
     1501 attatgttaa ataatttact tacaacagtg caaacccctc taaaatgtat tggcctacta
     1561 gctccacata aaccttctgt taatccagtt atattaacaa aagtacttgc attgtcacaa
     1621 tacatctctt caacttcagg attaaatatt gctgttgctc tacctcttgt gcatacttct
     1681 atatctcttt ctaagtctct tggataacta cctaaatctt catttttccc aaactgagtt
     1741 ggatttacaa agatacttac aacaacttta tcattttctt ttgatgcttt tctaattaaa
     1801 ctttcatgac cttcatgtaa atatcccata gtaggaacta atcctacctt taagccttgt
     1861 tttttccact cttttacttg acttctaaca tcatttactg ttttaaatat aaccatattt
     1921 cttgcccctt ttaatataat ttttctaaaa catcatcact tattttaaac ccatgttctg
     1981 gacctggaaa aactccatct tttacttcct ctatatattt tgtaaatgct ttattcatct
     2041 cttctgataa attagcatat ttcttcacaa atttaggggt aaaatcacta tacatcccca
     2101 acatatcttg atatacaaga atttgtccat cacaccctgc acctgctcca ataccaattg
     2161 taggtattga tatctcttta gaaattaact ctgcaagctt agttggaaca cattctaaca
     2221 ctacagcgaa tgctcctgct ttttctacag ctttagcagc tcttattaat tctttagcag
     2281 cttccttgtc ctttccttgt accttaaatc ctccaaatgc attaacagat tgaggtgtta
     2341 aacctatatg cgccataaca ggtatagaag cctttactat agcctctatt ttatcgcata
     2401 cttctatccc tccttctaat ttaacaacct gagctcgtcc ttcttttatt aatcttcctg
     2461 cattaactac tgaatcatat actgaagttt gataagacat aaatggcata tctgcaacaa
     2521 ctaaagtgtt cttagctcct cttgtaactg ctctagtatg atgaatcatg tcttccattg
     2581 ttactgaaag tgtatcttca tgtcctagac ataccatacc aagagaatct cctactaata
     2641 ttccatttat cccagcttca tcaattattt ttgctgttga atagtcataa gctgtaagca
     2701 ttgttaattt ttcatttctt aatttagatt ctttaaatgt aactgctgta tttttcatct
     2761 atttctctcc taactctctc ctaaatactt ttctaattca ctataatctc tattaatatt
     2821 ttttaacata gctatctcta atacattttt tgataatagc atatataaag ctctgtcttc
     2881 atctgataaa acttcacagt gacctttaat tgttgaaata tcacaacgtt caactggccc
     2941 tgtcaaactt tctataattc ctttttcttt tatatttcta atattaaatt caattaatgg
     3001 atataaagct tcaattgcca ttttttcagt aaatccacat tgaattagat atgtaacgct
     3061 tgtattaata agtcctagca caaggttact agatactact gatgcggcat gataaagaga
     3121 tttattctcc ttagatacaa ttacagtact attacctaac tttttgaaga aatcacataa
     3181 aaactttgca tatttttcat cgccttcaat tgttataaaa gattttttta attttttgta
     3241 tgaattatat ttatctgata ttgaaaacat aggatggaca gaatagccat aagcaccata
     3301 gtcctgtata tttgaaaaga tatctgaaga tattgcacca ctgcaatggc agattaattt
     3361 gtttttgatt tgataatttt taatttcttg ccatacctcc tgaatttgac catcaggagt
     3421 cgtaatgaaa atagtatcat tttcatctac taattctctt aagtttaaat attgtttact
     3481 agatgtaaaa ttcgaagctg ctaatgacga gtcttcgctt ttactataaa atcctgttac
     3541 ttgtatttta tgttcctgga aatacttccc gaatgaacaa cctaccttgc cggctccaat
     3601 aaaaccaacg ttcataatta tcacctccat aaagaagtga gactacaagt cccattctta
     3661 cgatatgagc ttaaaaataa aaattaaatc aaatatacag tatggttttt tcaaatatca
     3721 tccatagtta atttatcata atatcccata ttttaaaata aaagatagcg taaaatattt
     3781 tgtgtaaata gaaatttagg gtagaaaaaa aagtccattc tgtaaaatta aagttaccac
     3841 acagaaattt tataggagga ctttctcatg actaaattta ctatagaaat tatgcaaaca
     3901 ctactcaata aaggggattt agatgaatta tttcaggatc atttagagcg agcgattaat
     3961 tcacttctac aagcagagct aactgctttt ttagattacg aaaaatatga tcgaaatgga
     4021 ttcaattcag gaaattcccg taacgggaat tattcacgta catttaaaac agaatatggc
     4081 gagttgaatt taactattcc tagagatcgg aatggcgaat tttctcaaca aacactacca
     4141 gcatataagc gaaccaatga ttcattagaa acgaccatta tccaattatt tcaaaaaggc
     4201 attactatgg ctgaaatctc caaattaatt gaaaaaatgt atggtcatca ctatacgcca
     4261 caaacaatct ccaacatgag taaattggtg gctgaagatg ttttagcttt taaagaaaga
     4321 acgttagaag ccaattattc cgttatattt atggatgcga cgcatatccc tgtgaagcga
     4381 cagactgttt cgaaggaagc agtgtatatt acgattggta ttcgtttgga tggaaccaaa
     4441 gaagttcttg ggtttactat tgccccaact gagtctgctt atatttggaa agaagttctt
     4501 caagatctta gaaaacgtgg gttagaagaa gttttattag tagtgacaga cggattaagc
     4561 tgtattgaag aaagtatcca tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg
     4621 catgtatcta gaaatatcgc tcataaagtt cgtgttcgag atcgaaaaga aatttgtgag
     4681 gatttcaaat tggtttacca agcgaattca aaagaagagg cattggatca catcgacttt
     4741 atgactagga aatggaaaaa gcagtatcca agagtcgtca atttactctt gaatcctgcc
     4801 ctattaacct tttataattt ccctcacgcc atcagacgaa caatttattc gacgaacctg
     4861 attgaaggct ttaataagca gctaaaacga tatactcgaa gaaaagaaca attccctaat
     4921 gaagaatctc tagagagatt cttcatttct caatttaatc aatataacca aaaattttta
     4981 ggtagaattc acaagggatt taaagaaatt caggatacat tagagtcgat gatttaactg
     5041 taattaacag aatggatttt ccatttacac ataattcttg acgctaccat ttttagtcaa
     5101 gtccacaatc ttgtgtaaaa gttcaacctc atcaaaagca caatgttttg cgttgtgaac
     5161 ctaactgcca tgcctttctt tctgaggaga gaggtcagga gcagtagcgc agcgactctt
     5221 gatgtctctc ctcagaaaga agagactcgc acaggttcat ggtgctaaat gttattcgaa
     5281 ttaaattcta aatattttcg tggagaagcc agccactcag tgtctttttc catcaaaagt
     5341 gttccaatta tccgagtgat gga
//
LOCUS       DACDLI010000119         5348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDLI010000119 DACDLI010000000
VERSION     DACDLI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.119"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            193..573
                     /locus_tag="IV353_002598"
     CDS             193..573
                     /locus_tag="IV353_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9010770.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            555..1469
                     /locus_tag="IV353_002599"
     CDS             555..1469
                     /locus_tag="IV353_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9010771.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            1709..2398
                     /locus_tag="IV353_002600"
     CDS             1709..2398
                     /locus_tag="IV353_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYKEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2519..2605)
                     /locus_tag="IV353_002601"
     CDS             complement(2519..2605)
                     /locus_tag="IV353_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9010773.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
     gene            3250..3987
                     /locus_tag="IV353_002602"
     CDS             3250..3987
                     /locus_tag="IV353_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9010774.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            4016..4537
                     /locus_tag="IV353_002603"
     CDS             4016..4537
                     /locus_tag="IV353_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9010775.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENN
                     NAITTEKKWWKFW"
     gene            4621..4925
                     /locus_tag="IV353_002604"
                     /pseudo
     CDS             4621..4925
                     /locus_tag="IV353_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         2029 a          887 c          965 g         1467 t
ORIGIN      
        1 ttttgagggg gattcaaggg ggagcttgcg cccctttgaa aagcggttat ttgctagcca
       61 aatacgtagg tatttggcta gcaaatatag agcttgccaa acccttttag tgagctttac
      121 tcattgtacc tttttaggta caatggtatt gcgaacgata cgtccgtagg acgtactgaa
      181 agggtgggac ttatgagcga acaaaatcaa aatttggcta gcgatcattc taaaaaatat
      241 acctatcgat ccgagccaaa acaaatcagt tttcgagtga gcgaatctga atttgcaaag
      301 ctgaagcagt cagctgaagc tttgcaaatg agtgtgccgg ctttcgtaaa agccaaggca
      361 caaaacgcgc gtctggtgac accgaaagtg gcgccagaca ttgcccaggc aatcgcgcgc
      421 gatttagcca aagctggagg gaatatcaat caaattgcta agtggtgtaa tacacaccaa
      481 cacgacgttg ctcctggaga cgcccaacgc ttatccgaaa acctaaaaat catgcaaaag
      541 gagctacaaa agatatggca acagttaaag taagccgaac cacttcttgt agtcgtgcaa
      601 ttaattacgc tgaaccacga gcaaccatta aaacaggaat aaactgtgac gtcgattatg
      661 ccaaaagtga aatgaaacaa attcgcatgc tgtatggaaa agacgaccgt gtacaggcac
      721 acttattgat tcaatctttt cgaccaggag aaattaccgc tgaaaaagct aatcaactag
      781 gggcagagta tgcaaaaaaa atagcccccg atcaccaaat agctatttac acacatacag
      841 acaaagacca tattcataat cacattgtca ttaactccgt taatctggag actggaaaaa
      901 aatttcaagc tcatggtcaa gcatttctaa acaaatgtta tgacctaaat gacgagattt
      961 gtcttgcaca taacttacca atcactgagc gaggaaagaa accagaaaaa cgaaccatgt
     1021 ctgaaataaa attaaaagaa aaaaatgaac ctgtttggaa agacgaaatt cggattgcga
     1081 tcgatcaaac aatgaaaaac aaaaaaacca gaacctatga ccaattttgt aattccctca
     1141 aaacattggg gattcattgc ttcaatcgcg ggaaaaattt cacttacgaa ttgatccaca
     1201 aaaagaaaaa agtacgatcc aacagactag gaaaggacta cgaaaaggag acgattttac
     1261 atgagctgga cagacgagaa cgaacctata accaacagcg aaccaacgag ctacgaagag
     1321 ctcgagctag cattcgcgaa actgcagaag aagtacgaag agaacaacga actcttacgc
     1381 ctgaacaacc ggaaattaga ccggctcgaa gaaaccatga acaacacgtt gaaagaggaa
     1441 cagaccacca acaagaatta tcgctctaaa acgttaaaag acatagaaaa cgaaataaag
     1501 aaacaacgtg cttttctcgt tccttatacg caagaaagga tacaaactac gattgattta
     1561 caagttaaaa aactccatca agagctatta gaccagcgaa ataaggaaca gaaaaaggtg
     1621 cttattcaac gatcattgga ttttcttgcc cccttatgtt gcttagtcct gacactgttc
     1681 gcattttggt tagtatgggg aggctaaaat ggcattttat ttaccctatc tactcatttt
     1741 tgttagtatt tcaggaagta tttggctcat ctataaaatt tttcaaacaa gatattcatt
     1801 aaaaggctct aaaattcgat ttaagcgatt tttctttctt ggctgtatat tttccttgat
     1861 aatcgtttca agcggcttat tcggcgtttt agagggaaat aagcgcgttt ctagaagtat
     1921 tttattagga aatgtcactc aaaagtatga atcagcgcgt gataaaaaga aaaaagagca
     1981 agcattagca caaaaaatgg aagagtttac cacttgttat aaggaaatga acgatatttt
     2041 tgtgaatcaa gaaaaacgtt tgacggataa aaacatggaa aaactcactc gtctttatca
     2101 aaacttgcca gaaaaacagc aaaaagaggt tcaagacaac tatgagcgaa ccaaaaaaga
     2161 cgtgcaatat gtaaaagata caaagataga agaagcctgt agtgatctat ttggagacac
     2221 aaacccttgg tttgccagtg aagaagaaaa aaaagaaaaa caacaatctg tcacatatga
     2281 acgctacgaa aacctgtttc aacaagcaac taacatgcaa aatccgacaa aaaaagaaac
     2341 cgccttaaac tatctaaaaa gtgtcaagga atggttagat cagcaacagc aaaattgaat
     2401 ttgattgaaa ctcatgttaa actgaatgtg caaatgatca aacattagat acacgaaagc
     2461 cccagagagg tcggattctc tggggctttt tttcaggttg atgtcgcctt tactgatttt
     2521 acttcttgtt tcggttattc aaccaataac tgaataaaga gacacaaact cctacaagaa
     2581 gggggcaaat gaactgttca aacattagat acacctcctt ccagtcgcaa gactgtcgga
     2641 taggcgacaa gttattatac cctacaaaat taaatctgtg tggcgatttt aagtatacct
     2701 tattttcggg ttgttagggc ttgccctgac cgcctgtaag gcgcttctcg actagattaa
     2761 gggagttaat caagaattgt cttagaacgg cttagacaag ctctcagatt ctcccacctc
     2821 ctctttgaac aacggtttta gcaaaggagc cggaggcgac tgcgcaggta caatgtgaga
     2881 gcgtagcgat cacacctttt tagcgaccgt agggagcaag ttttctagtt tagaaaaatt
     2941 cgcgaacctt tttggaggta aaagtaggag ttttttaggc gggaaattaa atgtgctctt
     3001 aagatatact gttagatata ctgttagata tactgttaat aataactgtt agggattagc
     3061 aaccttatgg gagagtaagt attgacatag gtagtggggg ataaattgtc acactatggg
     3121 ggataaattg tcacactatg ggggataaat tgtcacgcta tgggggataa atcgtcacac
     3181 tatgggggat aaattgtatt ttacaccctc ttatgtctat gataacataa tttattgagg
     3241 tgatttttat tgagtaacga tatagttcga tataataatg gattaaattc tgtcccttta
     3301 agaaaattta cccctataga tatggattta ttttggggaa tatgttctaa aatgaaaaga
     3361 aaaggtacaa aagaagaaat ttttacattt aaacaaataa aagaattaat tgattacaat
     3421 ataaatgcac gtggcgaccg ttttgctaat gatttaataa aaatgtcaaa aaaaatcatt
     3481 actttgtctt ttatttatga agacgaaaaa acatatgaac aattaaatct gtttcaaaaa
     3541 tttaaaatcg ataaagtgaa tgaaacatta ttaataaagg tttctgaaga atttgaatat
     3601 atacttaatt caatcggaac taattttact cgtttcgaat tagaaaatat gacaaactta
     3661 aagagtagct acgttaaaga gttatatcgc caacttatgg ctcataaaga cagaagatct
     3721 aaaaaaggtc actggtttgt aaaaatagaa gattttagag ctctattagc aataccagaa
     3781 agttatcgca tgagagatat tgataaacaa atttttgccc aagcgaaaaa agaattttta
     3841 aacgttaatt caactcaccc tgctattttt aaaacttttg aaatcaaaaa aattaaagcc
     3901 tacaaaggaa ataagatagg atctctgcaa atttattttg aagaaaatat accttcaatt
     3961 accttagata attggttaga gaaataataa cctaacaatt taggaggcaa ttatcatgca
     4021 acccaaagaa acctttacaa taaaggaatt atctgaatta tttgaaataa gtaggcaagc
     4081 aatttctaaa catgttgata aattagaccc gtccttatta gctaaaaatg agcgtggtta
     4141 taaggttgta ttacgttctg gagtattgca acttgctagc aacttaggta accaaaggtt
     4201 actagatagg ttgcatgagt atgaacaaga aaacgataaa aacgtttata tatcaagaaa
     4261 aaacgtggtt gtaaacgagt tgctagataa gttgcgtgaa gaaaatgaat ttttacgaga
     4321 acaactttct ctcaaagagc aaaataattc taatatgcaa aaattactcg accagcaaca
     4381 acaactcacc ttgcaggcaa atcaacagat cgaaaaactg caagaacagt tacaattagc
     4441 ttatacggaa gaacctgaag aagaggtatt aacttctgtc aataaagaaa acaacaatgc
     4501 aataacaaca gaaaaaaagt ggtggaaatt ttggtaacaa ttaccaactt tccataaaga
     4561 agaaatcaat ttctatagac tttatctttc taaaaataaa aaatatgttt aggtgatcaa
     4621 atgactataa gtaaagtaat caaacaaaaa agaatagaaa aacaactaac tcaagaagat
     4681 ttagctgaaa tgcttttagt atctaaaaaa accatttcta attgggaaaa tggtagaaca
     4741 ataccagata cagaaaattt aattcaattt gctaagttat ttgatttatc actagacaat
     4801 attttgttag gagaagaaaa agttatgtta aaaaatcata caaaaacact aatactatta
     4861 tttcattttg taattttttt aagtttagtt tttaccttaa ttcagtccaa attcttttat
     4921 tttagccatt caattgccac ttggttatta atattttttc taagtttgct attagtaatc
     4981 aatactctga ttattatttt taataaacat aaaaatttat ttcttcatag tgtttttcca
     5041 attattttgc tgagtttttt atactttgta taaagtataa aaaaagactg ctctttcgag
     5101 cagccagtta aaaaaataat gagagtttat tcttgaaaaa gcaacgcgct tataaagcgc
     5161 taccaaaatt ttatcatatt ttataaaaaa taacaatcca gtaaaaaatg actggtaaaa
     5221 attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa aagcttttga
     5281 cctagaccaa tgaaaaatca caaagtgagc agctatagcc cttgatagag gggctacttg
     5341 gcatagtc
//
LOCUS       DACDLI010000120         5345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDLI010000120 DACDLI010000000
VERSION     DACDLI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.120"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..939
                     /locus_tag="IV353_002605"
     CDS             <1..939
                     /locus_tag="IV353_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9010776.1"
                     /translation="EWKETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLA
                     IDILTFLTGISSRDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHI
                     ESFVDYDFAHLLFSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAM
                     LIVISTFALDFIHSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVT
                     QNADDLLDEKMKNNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCL
                     VSDIYGRVGVMQFHPIFEELLHAFDTRPPVRNEVEE"
     gene            936..2963
                     /locus_tag="IV353_002606"
     CDS             936..2963
                     /locus_tag="IV353_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010777.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            2960..3982
                     /locus_tag="IV353_002607"
     CDS             2960..3982
                     /locus_tag="IV353_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9010778.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            3999..4928
                     /locus_tag="IV353_002608"
     CDS             3999..4928
                     /locus_tag="IV353_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9010779.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            5173..5289
                     /locus_tag="IV353_002609"
     CDS             5173..5289
                     /locus_tag="IV353_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9010780.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1755 a          948 c         1091 g         1551 t
ORIGIN      
        1 gaatggaaag aaaccttacc tgatattgcc catgaaatca atattgttaa tctcacgagt
       61 gagaacgaca ataaagggtt acttgaccct tatgtgattt taaaactccc gaaagattca
      121 gaaagccttg ccattgatat tctcacgttc ttaacaggta tttccagccg agatagcgag
      181 aagtttcctg tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca acgagggttg
      241 ttattggtga ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag tattgctgac
      301 catatcgaaa gctttgtcga ttatgatttt gcccatctgt tattcagtga tggcagtgtc
      361 acccaatcca ttagcttgga aaaacaactc aatattattc aagtggcaga tttggtctta
      421 cccgattcca atagctcttt tgaagaatac actaccatgg aattattaag tgtggcaatg
      481 ctgattgtca ttagtacctt tgctttagac tttatccact ctgaccgttc catttttaaa
      541 attgttgacc ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa agctctatct
      601 atgcgattgg ttcgagcagg acgtgccatg aatgcagggg tctatttcgt tactcaaaac
      661 gcggacgatt tactagatga aaagatgaag aataatattg gcttgaaatt tgcctttcgt
      721 tccactgacc tagtggaaat caaaaagacc ttagaatttt ttggtgtgga caaggaagat
      781 gaaaacaatc agaaacgctt acgtgaatta gaaaatggac aatgtttagt ttcagacatc
      841 tatggtcgtg taggggttat gcagtttcac ccgatttttg aagagttgct tcatgccttt
      901 gataccagac cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa aagaaatttc
      961 ttcactatag ttggataacg ctcctttgtg tgggcgttat tttactttta ttaggcacgc
     1021 tgggcaatgc agtacaagcg acaggcttag ttgatgaaac cattgataca agcaatgaat
     1081 actccaagta tggtttgaat caatatcagc ttgattatta tgtggacaat agctggggct
     1141 ggcttccatg gaattggagt gatggcatag gacaatcggt gatgtatgga ttgtatgcca
     1201 ttactaattt tatttggaca atttctcttt atctatcgaa cgccacaggt tatttggtga
     1261 aagaagccta tgccttagat ttcattagtc aaacatccca agcaattggc gaaaacatgc
     1321 agacgattgc aggcattaca ccgaatggct tttccagttc agggttttat gtcggtttcc
     1381 tccttctttt tattctcatt gttggaatct atgttgccta tacaggattg gttaagcgag
     1441 aaacgacgaa agtgatacga gctgttttaa actttgtcgt ggtgtttatt ctatcggctt
     1501 cctttattgc gtatgcaccc gattatattg cgaaaatcaa tgatttttcc aaagatgtca
     1561 gtaccgccag tctgaatatt gggacaaaaa ttgttcttcc taacacggat agtcaaggga
     1621 aagacagtgt ggacatgata cgtgacagct tattctctgt tcaagtaaag caaccatggc
     1681 tattgcttca atatggctcc actgatgtag aagcgttagg ggaagaacgt gtggaaagtt
     1741 tattgtctac cagtccagat acaaacaatg gtgaagatag agaaaatgta gtgattgaag
     1801 aaattgaaga tcatgacaat acctatttaa ccctaccgaa gacgatttcc agattaggaa
     1861 cagtcttctt tttgtttgta ttcaatattg gtatctcgat ttttgttttc cttctaacag
     1921 gaatcatgat tttttcgcaa gtgctattta ttattttcgc tatgtttttg ccaatcagtt
     1981 tcctattaag tatgttgccg agttttgatg gcatgggcag gcatgccatt accaagttat
     2041 ttaatgtgat tatgacaagg gctggtatta ccttgattat taccgttgcc tttagtattt
     2101 ccactatgct ttattcgctg tcagcaagtt ctcccttttt catgattatg tttttacaaa
     2161 ttgtgacctt tgcagggatt tattttaaac tgggcgattt aatgagccta ttcactttac
     2221 aaagcaacga ctctcaaaac atgggcagac aagtgtttcg cagacctcgt caaatgatga
     2281 atcgacaaac cagaagattt caacgcagta tgagtaagat ttttcgtgga aaagcagggt
     2341 caacgaagca aaagacaatg acaacaaagc agaatcaaac caataaagct aaccatactc
     2401 gtcaaaatga acggactaca ccaaagaaag aaacagcact ttccaatgtg aaaaagcaac
     2461 aaggcggtat cgggcaacga ttaggtgaaa agacagccca tgtattggac acgaaaaatc
     2521 gtctagtcaa taaaagaaag caggcaaaac aacagataca ggcgacacca acccatgtca
     2581 aatatgccct tcatcaagga aaagagaatg tcactcataa tgtaacagac tttaaacaat
     2641 ctatggcaga taccaaagct caacgcaaac aacagcgtat gaaccagcag gagcaaaaac
     2701 gaaagaacat tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa agacgaaaag
     2761 aaaacccatc atctcctgtt cagaataacc agcagcatgt tcgcaagcgt cagcaaaccg
     2821 tcacgcctgt tttgtcacaa aacactaaac gtgaaaatca tcagcgacca ttgaaaaaag
     2881 agcaaaaaca aaagacacca ccgaagcaat tcaatcagag gaagctaaat aaaagaacgg
     2941 ataagcgagg acaacaaaca tgaagctaaa aataacagtg atttgtagtg tggtcttctc
     3001 tctcttcttt tttctcttat tattcctagt cattttcttt gcagatgata cggacagtgg
     3061 cgaaaacaac aaggattctt ctattccaca aggcggtgtg accgtttcac cagaagtgtt
     3121 ggcacaccgt ccgttgattg aaaaatatgg taaggaatat gggattgaag actatgtttc
     3181 ttatatctta gccattatgc aagtggaatc gggcggtaca gcagaagatg ttatgcaaag
     3241 ttccgaatct ttgggtttac cgcccaacag tttgagtacc gaagagtcca tcaaacaagg
     3301 ggttaaatat ttcagtgaat tattaaccag tgcagaacaa caaggagtgg atatagattc
     3361 tgttatccaa tcttataatt atggcggtgg ctttttaaat tatgtgagga gtcatgggaa
     3421 aaaatatacc tatgaattag ccgaacagtt ttctaaagag aagtcgggtg gtcaaaaagc
     3481 agactatccc aatcctatag ccatacctgt gaatggtggt tggcgatata actatggaaa
     3541 tcagttttat gttcagttgg tttcacaata cctaaccgac acttccccaa cagaatttga
     3601 tgatgaaacc gtgcaagtca ttatggacga agccttgaaa tacgaagggt ttccctatgt
     3661 ctttggaggg gcgtcgccta ccacctcttt tgattgtagt ggcttaatac aatgggtgta
     3721 tgacaaagct ggcatttctc tccctcgtgt cgcccaagat cagtatgacg ctacgcaaga
     3781 aatctctatg gaagaagccc aagcaggcga ccttatcttc tttcattcaa cctataatgc
     3841 aggcacatat gtgactcatg tggcgatata tttagaggga aatcgctttt atcatgcagg
     3901 cgacccaatc ggttatggcg acttaagcag tcgttattgg caagaccatc tgattggggc
     3961 tagacgtgtt attcataact aagaaaggaa tgaatcaaat gattataaaa atcgagcgaa
     4021 aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag caagcaaaga aaagttccga
     4081 tgattaaagt tggcacgcat aagaaattaa cctttgtttt atgggtttta ttgattggta
     4141 gtgtcggatt tggaatctat aaaaatttta ctgccattga cactcatact gtccgtgaaa
     4201 cagaaatcat taaacagcag attgtcgata ccaaccaagt agaaagtttt gtgaaatcct
     4261 ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc cattgataaa cgaaatgaaa
     4321 aattgaccca ttatttgacg gaagaattgc aggtattaaa tgaagaaatg attcgtaagg
     4381 atattcctac tagttctagt gtgaacgata tacaggtgtg gcaggtttct caagtgaatg
     4441 agaacacctt tgaggtactg ttttctgttg aacaagttat taccgaagac aaagacaagg
     4501 aaactatatc gtctagcttt catgtggttg tccatataga tgaatcagat aatatggtta
     4561 ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc
     4621 tagaaagtga ccatacagta gatacagaaa cgatggacga aatcactagc tttttagaaa
     4681 cgttcttcca gctctatcct accgccacag aaaaagaatt gacttactat gtaagtaatc
     4741 atgtgttacc tatgattaac aaggaatatg ttttcgagga attggtgaat cctatcttta
     4801 ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa atatttagat caagaaacaa
     4861 aggcaaccca aatctcgcaa tttgagttga tattagaaaa acaagataat tggaagattg
     4921 taaaataaca aatattggta catgattaca gatactttgt aatcatgtac tctttttgat
     4981 aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa gtgactattt
     5041 aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagcgg
     5101 gaacaaataa ttagatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa
     5161 tacctttatc atgtgattct aaagtatcca gagaatatct gtatgctttg tatacctatg
     5221 gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg
     5281 ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt
     5341 gatgc
//
LOCUS       DACDLI010000121         5333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDLI010000121 DACDLI010000000
VERSION     DACDLI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.121"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(240..458)
                     /locus_tag="IV353_002610"
     CDS             complement(240..458)
                     /locus_tag="IV353_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010781.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(473..3046)
                     /locus_tag="IV353_002611"
     CDS             complement(473..3046)
                     /locus_tag="IV353_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010782.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTRNVVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVMNTIVVGLMILSLMFVGYKMITGKGTIDLKSVGTNIVMSVALI
                     LLMPTMISSGIQFSKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVE
                     DRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLLK
                     K"
     gene            complement(3266..4150)
                     /locus_tag="IV353_002612"
     CDS             complement(3266..4150)
                     /locus_tag="IV353_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HAP9010783.1"
                     /translation="MNFMAEKRMLAKSFLNNASFMKMSDSAKLLYVYLNTNADDDGIV
                     EAYPYMKMLGTPDDDVKILFTKGFVFFLNEDLLTFLPHWLQNQNIRIDRYKPSIYRFK
                     LLNLKPEFFSKLKPVKLDVILKSGQVEPLRGDLMEKWYPAGIPSDVKSDTQLSQGQDS
                     QDQTNQDKDSIEEINQDQNSFFENIINQKGIEIITTLGDRYTEVQREKYLKLIASMKD
                     SIEREYKIEIRGQDYFDITNKMLTRLFNSINEKLNLGEKIDEMKFINTTARNHWEDIA
                     KQEKASRQDMLEALKKIG"
     gene            complement(4170..4371)
                     /locus_tag="IV353_002613"
                     /pseudo
     CDS             complement(4170..4371)
                     /locus_tag="IV353_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4416..4688)
                     /locus_tag="IV353_002614"
     CDS             complement(4416..4688)
                     /locus_tag="IV353_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9010784.1"
                     /translation="MLEVILPPDLDRELRNYFYHVAREAVDQVRIETSFNKEVFRLGE
                     ACEYLSVSRSTLKKFIREKGLKVSVINDCQFIRKEELARFIKEHEV"
     gene            complement(4702..4902)
                     /locus_tag="IV353_002615"
     CDS             complement(4702..4902)
                     /locus_tag="IV353_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9010785.1"
                     /translation="MLAEIIKIKRIERGLTQEELARIVGISRSYYSDIENNRTIPSGK
                     VLLNLNKTLKFFKINDVNNGIK"
     gene            5019..5333
                     /locus_tag="IV353_002616"
                     /pseudo
     CDS             5019..5333
                     /locus_tag="IV353_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1557 a         1067 c          708 g         2001 t
ORIGIN      
        1 atatacatta ggttcaactc ttttgcaggt ttcatgggtg tactatcact tttaatctga
       61 tagatgtaaa cctctcttta tctttttgtt acttcaattt tcaacaatag ctcctttata
      121 ggttgtcaat attgctctac gtattctggc tttttaaccc attatttctc taaagtggaa
      181 gagaataatc gcttaatccc cactaattcc ttgtcgtaaa ttcatgtcaa atacaatttt
      241 taaaaaccat ttaattcatg gattatctct tcactttttt ctagataaca atatttcaag
      301 ttgtttttta tctcatgttt catctttaat tcttcttgaa tcatcagttc aatataatta
      361 gtgaccgtga ttttttcatt tgttgcgttc tttactgatt cacataattc gtcaagttct
      421 aagaaatcat gcgtactaat ctttagaatt aatttcatga caattccccc ttttattttt
      481 ttaatagatt atcgaaaagc ggattaggat cattaagtgt atctctgaat aactcgttgt
      541 cttcataagt gaatcttcgt cttggttttg atcccttatc attttcacgg atttcactat
      601 caataatcac tgtttcatga tttgaatcaa attttttcgc tgactttctg tcttcaacga
      661 tattgatttt ttgtgttttt tctttcattt ctccactatc tttttgaacg ttctcccgaa
      721 catctactgt tttgaatcct tgactacttg tagaactttg tacattctct cttacatcca
      781 ttgatttaaa cccttgacta cttgtagaat tttgtacatt ttctcgaaca tcaattgtct
      841 tagtaccttg accatttgaa gagctttgta aattctctct tacatcaaca ttccgtccga
      901 catgaccatt caatgaacct tgaatatttt ctcggacatc tactgatcgg ccaccttgac
      961 tacttgtaga attttgtaga ttttctttga ccaatttttc taatccggta tttgagtttt
     1021 tagcttcttg gattctttct ttggtcaacg cttctttttc gtccgtatta gctcctgtac
     1081 gcccttctac acgtttcctt aacatttcct cacgattcat tgcttcaggc gtgtatttag
     1141 cttcttgtcg cttctgatta attaattctt cagcagactt agcttgaccg tgcatagcat
     1201 ttttacgttc ttcaatatct tgttgaactt tttgctgaat tgcacttaca ggattattat
     1261 tcgattcagc attatttttc attgcttcac gcatacgttc agaactagac ataattctat
     1321 cggcattttc aaccttagca ccatcttttc tatccgaaag acgtgttggc atggaatcag
     1381 catattttcc agtttcatct tctttatttt tagctaacat aggtttgcta tttttattaa
     1441 tagcggacac tgtaccatca tctaaacctt ctttgaactt attcttggta tcattagcca
     1501 tatcacgcat actcttaaat ggttttattg ctctttcact agccattgta gctccatctg
     1561 aagctaaacc tgataatcca cgttcatggg catatcccaa agctcttcca gttttgccag
     1621 ctttttttga tagagtttcg ccgaaattct tttctggttt tcgatcttct ccttctccac
     1681 tgtctgatcc atttccattt ccgcttcctt tagcacgatt aaatccagtt gatccttttc
     1741 taaacagcat tgcgcccata ccaaaagcag aagcaagttg cccatagcct tctttgagtc
     1801 caatatcaac accaaagtat ctcatgactg ttccactgcc ttttatcagc acaaacacag
     1861 cacagatata agcaattgtt tttataaaaa tatttgtgga gataccttgt ccagcattta
     1921 aaaaattgat aaacatagca aacatagata gtccaaaccc ttgaaatcca actgttaagt
     1981 aacattgtaa aatatcggat aaaacaactt ttgaacgttg tcctgtttca atatctgtcg
     2041 caaaaacaag aacacctaac acacgtttaa atgcaagttc taatattgct gtaatgatca
     2101 caaacgcgct gaacacatag gcgattgcta acgctgtcaa tccaatcgaa atcccccaaa
     2161 gatttgcttg atagcgataa tagcctgatt tcaaactatc agaaaatgtt gataagaagt
     2221 tatcagagaa ctttgtagca acaaattcat tattagaatt ttcaactaat tcatagcgaa
     2281 gattatccgc agctggattt tctttttcca atttatcaat cactttatcc gttaacactt
     2341 gtgtgagatc cgtctgttga aaattttttc gtgttaactt atttcgatcc tccgttttat
     2401 caatgcttga gtactgatct gttttattaa tatatgccaa atctgttact ccttgtttta
     2461 tcaatgacca agcgactccg tcatctgaat ttgtaatcgt agtagcatca ttgtaaaata
     2521 ttttggaaaa ttgaataccg ctagaaatca ttgttggcat taaaagaatt aatgctacag
     2581 acatcactat attcgttcca acagatttta aatctattgt tccttttcca gtaatcatct
     2641 tgtagccaac aaacatcaat gaaaggatca ttaatccaac gacaattgta ttcatgactg
     2701 attgataaac gttatttagc cctgtacttt caatgaaatt gaacaaatcc agcacatctg
     2761 gaatcatatt ttgaatcgaa tcagtcacag taaacatgcc ttttaccaca gcccatccaa
     2821 gccaaatccc gaactgacta aggaaatctc cattgtattt tttcaggtaa ggtctataaa
     2881 gagaattatt caaaaactct gtttgacttt ttgtgtcatc aaaccaattg aaaatgctag
     2941 cttcaacaac attccttgta aaaacaacgc taatcattaa aacaagcaca ctgagtgcta
     3001 accagtttat ctttctacta acgatttttt ctttaattgc tttcatagtt ttctccttca
     3061 gcccattttc ttaggctctc agagagctta tgagccgttt ttctgtcaat tatgatgtga
     3121 gtgacgtaac aatcgttttt agcctttaaa tcgaggacta tagtattgaa acagatactc
     3181 tcgattaact caagagtatc tgcgttatga tcaagacaat aaatttgata tattaaccgt
     3241 ttttcaatca tattattaat cctttctaac ctattttttt cagtgcctct agcatatcct
     3301 gtctactagc tttttcttgc ttagctatgt cctcccaatg gtttctagct gtggtgttga
     3361 tgaacttcat ttcatcaatt ttttcaccga gattcagttt ttcgtttata gaattaaaaa
     3421 gtcttgttag cattttgttt gttatatcaa aataatcttg acctcgaatt tctattttat
     3481 attctctttc aattgaatct ttcatactag caatcaactt aagatacttt tctctttgaa
     3541 cttctgtata tctatcacct agtgtagtga ttatttcaat tcctttctga tttataatat
     3601 tttcaaaaaa ggaattctga tcttgattaa tctcttctat actatcctta tcttgattag
     3661 tttgatcttg actatcctga ccttgactta actgtgtatc cgatttgaca tcgcttggta
     3721 taccagctgg ataccatttt tccattaaat cgcctcttag cggttctact tggccagatt
     3781 tcaaaatcac atcaagtttt actggcttta attttgagaa aaattctggt tttaagttca
     3841 ataacttgaa gcggtaaata gatggtttat acctatctat tcgtatattt tggttttgta
     3901 accaatgggg gagaaatgtt aacaaatctt catttaaaaa aaatacaaaa ccttttgtga
     3961 aaagtatctt tacatcatca tcgggtgttc cgagcatttt catgtatgga tacgcttcga
     4021 caattccgtc atcgtctgcg tttgtgttca gatatacgta taataatttt gctgaatctg
     4081 acatcttcat aaaacttgca ttatttaaaa atgatttagc cagcattcgt ttttctgcca
     4141 taaaattcac acttcttcct atttgaatgt taaaatgtag tcaaataaag ttttctcaga
     4201 actttattgt taagtgctcc cctttgtctg caaactatca ggatcgctcc tttttatttt
     4261 cttcaaagcg ttcttaatac ctttttctgc atattcccgt tgcagaatat caaactgcaa
     4321 gtacatctcg atttctttta gaatcaactt tgctacttca tcatcgttca aacaaacact
     4381 tcctttttca tatcaaaagc acgccacgca actttttata cttcatgttc tttgataaac
     4441 cttgctaatt cttcttttct aataaattga cagtcattga taacactaac ttttaatcct
     4501 ttttcacgta taaatttctt taatgtactt ctgctcacac ttaaatactc acacgcttcg
     4561 cccaaccgga agacctcttt attaaaagaa gtttcaattc taacttgatc taccgcctct
     4621 ctagcgacgt gataaaagta atttctcaat tctctatcca aatcaggtgg taaaataact
     4681 tccaatatta ctcctcctca cttatttaat tccgttattt acgtcattta tcttaaaaaa
     4741 ttttagggtc ttatttaagt tcaataaaac cttccctgac ggaatagtac gattattttc
     4801 tatatcagaa taataacttc tcgatatacc gacaattcta gctagctctt cttgcgtaag
     4861 ccctcgttca atacgcttta tctttataat ttcagctaac attacaatcc cccttccaac
     4921 gacgtacatt tacgtctaaa aatatcatac tgcactgcag ttcctaagtc aacaaaaatg
     4981 ttgtgtattt acgtcattta acatatacta ctttatatag tgtggaggtg aaatgtttga
     5041 ttgatattgg aaacaacatc aaaaatatta gaaaaagtaa aaaaatgaca caaactgatt
     5101 ttgctgagtt aatgggtcta tcacgatcat atatagggga tttagaaaac aacaggagta
     5161 atcccagtat aaaaacactg gaagccattg caagagcatt gaatgtcgac ctacaaaaaa
     5221 ttattagttc tgatcgaact ctaaatgcca aagataatac cctaaaaata atcgagtata
     5281 cacaattata taataataaa tatttattta aattggctgg tacggtgact cat
//
LOCUS       DACDLI010000122         5259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDLI010000122 DACDLI010000000
VERSION     DACDLI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.122"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1273..1938)
                     /locus_tag="IV353_002617"
     CDS             complement(1273..1938)
                     /locus_tag="IV353_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9010786.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IV353_002618"
     CDS             2064..4100
                     /locus_tag="IV353_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9010787.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IV353_002619"
     CDS             4104..4742
                     /locus_tag="IV353_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9010788.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IV353_002620"
     CDS             4770..5198
                     /locus_tag="IV353_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010789.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1745 a          799 c          957 g         1758 t
ORIGIN      
        1 aaaatgattt agaatatcaa aataacaaaa aagaggacta cctgtattcg tcctcttttt
       61 tgaccagatt ttgaccagtc taatttacat tctaatatat tcatatgagc taattttttg
      121 attaaatggt aagtttttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttattt
//
LOCUS       DACDLI010000123         5215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDLI010000123 DACDLI010000000
VERSION     DACDLI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.123"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IV353_002621"
     CDS             <1..106
                     /locus_tag="IV353_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9010790.1"
                     /translation="MKAIRGLYKKTRKEGALFGFSVCTEIKVLLGIPA"
     gene            161..610
                     /locus_tag="IV353_002622"
                     /pseudo
     CDS             161..610
                     /locus_tag="IV353_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(749..1294)
                     /locus_tag="IV353_002623"
     CDS             complement(749..1294)
                     /locus_tag="IV353_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin ECF transporter"
                     /protein_id="HAP9010791.1"
                     /translation="MLPTSNSRFPIRRITTLALFSAACIVGRILFTWIPNVQPMTAIL
                     LLLAFYGSYTDALIVTLVSLLGTNLYLGMGSWTISQLIAFSSIVTVFHFLSNCALIKH
                     HISIQAIIAFLCGLLYGLIVSRVEVFIYQLPSFWAYYFQGLFFDILHGTGNLIFYLVL
                     VSVFKRILLPAINSSKNKLNS"
     gene            1444..3927
                     /locus_tag="IV353_002624"
     CDS             1444..3927
                     /locus_tag="IV353_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HAP9010792.1"
                     /translation="MDYPKKYIEKLNQDISSYKEVAKITAEMTQTFDGISRLIMLDRY
                     AFKDMSGKTLTVGDLVILTTHADPQYPARGIGKIEEINKEQLIIRIEEEFQSSLTDPE
                     EIASGRVIRNINEVEKPLELYFEQIAARVARGISDIEGEKKAQFFTEFYQHLTEQNLI
                     PAGRVLYGAGTNSEVTYFNCYVMPFIHDSRGGLAKHRQEVMEIMSRGGGVGTNGSTLR
                     PKSAIVHGVNGKSSGSVSWLNDLANLTNLVEQGGSRRGAQMIMLNDWHPDIFEFIISK
                     IQNPAILQNLIHTSQSPTVRQLASEKLTFEPLTANEKEWLEFLVDHPTNDMEDKFLKT
                     AQQKLKAGGTYSVKDPDFLTGANISVCLTKEFMEAVETDQTYELRFPAIEDYDASEMA
                     DYDQHWMEVGDVREWAETGRKVKIHQTIKARELWDLITYCATYSAEPGIFFIDNANEM
                     TNAQAYGQKVVATNPCGEQPLTPYAVCNLAAINLANMVDKKTKVVDFEKLTDVVRASV
                     RFQDNVIDATPYFFKKNEQQAKGERRVGLGVMGLADLLIYCEKVYGSEDGNRLVDQVF
                     EKIAITAYQTSVELAKEKGSFPFLVGKTVEETTALRQRFIESGYMKKMPESIRQAILK
                     YGIRNSHLLTVAPTGSTGTMAGVSTGLEPYFSFSYFRSGRLGKFIEVNAEIVSEYLEK
                     NPEKTADNLPPFFVTAMELSPKDHVSVQCTIQRWVDSSISKTVNAPKGYQVKQVAAIY
                     EDLYQGGAKGGTVYVDGSRDSQVLTLEADGNVFEEPVVGVADNQITLDSLESSEAEEI
                     CPICHEGRIEEIGGCSTCTNCKVQLKCGL"
     gene            3941..4504
                     /locus_tag="IV353_002625"
     CDS             3941..4504
                     /locus_tag="IV353_002625"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAP9010793.1"
                     /translation="MKIYTKSGDKGNTSIIGGERIKKSDARVCAYGSVDEVNSWVGKI
                     ISELDDKKFELLKKELVVLQILLFDIGTDLATPIGIKEMIVGKEEVTKIETLIDFYQA
                     KVPVIEKFILPGGHPVACDMQITRTVIRRAEREITRLIVSDEPINLHAYKIMNRLSDL
                     FFVLARYVNHVYATKEPFYERAGKVFH"
     gene            4520..4924
                     /locus_tag="IV353_002626"
     CDS             4520..4924
                     /locus_tag="IV353_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9010794.1"
                     /translation="MKKLATLLTVFIGLFIMTGCQSNQNDQASSKASTQATSEVAKSS
                     ALIKLTDSDKEITSKTVTYKSGESLLVILKANFEVKERDGFITSIDGHAQDESQNKYW
                     TFTINGKMGEKGAGETTLDNDDQVVFNLGVFK"
     gene            5126..>5215
                     /locus_tag="IV353_002627"
     CDS             5126..>5215
                     /locus_tag="IV353_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9010795.1"
                     /translation="MSNFKGKQFEKDIIMVAVCYYLRYNLSYRD"
BASE COUNT         1837 a          809 c         1063 g         1506 t
ORIGIN      
        1 catgaaagcc attcgaggat tatataagaa aacccgaaaa gaaggcgctc tcttcgggtt
       61 ttcggtctgt actgaaatca aggtattatt gggaatccca gcttaaatca tagataccgt
      121 aagagatttt attctttatt taaaactttg caacagaacc gatgaaactt atattaaggt
      181 aaaaggaaag tggcactatt tgtatcgatc cattgattct tcggggataa cattagatat
      241 ttggttacga aaaaagcgtg ataaacaagc agcttatgct ttctttaaac gattggtcaa
      301 acaatttgga gaacctaaag ttcttgtcac tgataaagcc ccttcactaa gtagtgcgtt
      361 taatcaattg cattctgaag gattattttc taagactgaa caccgaacaa ataaatactt
      421 gaataatatc attgaacagg atcatcgatc catcaagaaa aggcacaaat tatatcagtc
      481 cataaggact gattcctcaa caattaaagg aattgaaact atacacgctt tatataaaat
      541 tagccaaaga gacaaaagtc tctttggctt ttcagtgatt catgagatga atacattaat
      601 gagtatctaa gcataaaatc aaaaattaat tggaaactct ttattaattt tttaactttg
      661 caacagaacc atcttaaata atacgcaagg cttcctattt gaattatagg tttaatttaa
      721 taagagatat tgtgatttag atactcatct aactatttaa tttatttttc gaactattaa
      781 tagcaggtaa taaaattcgt ttaaacacag aaactaatac aagataaaag attaaattac
      841 ccgttccatg gaggatatca aaaaataatc cttgaaaata ataggcccaa aaagaaggta
      901 gttgatatat aaagacttca actctcgata cgatcaatcc gtaaagaagg ccacataaaa
      961 atgcaatgat tgcttggata gagatgtgat gcttaatcag ggcacagtta gataaaaaat
     1021 gaaaaaccgt aacgatacta ctgaaggcga tcagttggct gattgtccaa ctacccattc
     1081 ctaaataaag gttcgtgcct aataagctta ctaaagtaac gattaaagca tctgtataac
     1141 ttccataaaa agcgagtaaa agtagaatgg ctgtcattgg ttgtacattc ggtatccaag
     1201 taaaaaggat tctaccaaca atacaggcgg cactaaataa agcgagggtg gtaatccttc
     1261 tgattggaaa gcgtgaatta gaggttggta acactaggaa aactcccttg ttttaattga
     1321 taatagttct cattgtatca gaataaaaat taattacaag attgaattac actttatata
     1381 gggctataat cattggcagg cacaatatgt tgtgtttgtt aatggtaaag gtggtattat
     1441 ttaatggatt atcctaaaaa atatatagaa aaattaaatc aagatatctc ttcttataaa
     1501 gaagtcgcta agataactgc agaaatgact caaacatttg atgggatttc aagattaatc
     1561 atgcttgatc gctatgcatt taaagatatg agcggaaaaa ctttgacagt cggagatttg
     1621 gttattttaa caacccatgc tgatccacaa tatccagcaa gagggatcgg aaaaattgaa
     1681 gaaatcaata aggaacagtt gataattcga attgaggaag aatttcaaag ctcgttaact
     1741 gatccagaag aaattgcttc aggacgagtg attcgaaata taaatgaggt tgagaaaccg
     1801 cttgaacttt attttgaaca aattgcagct cgtgttgcta gaggaatctc tgatattgaa
     1861 ggagaaaaaa aagctcagtt ttttaccgag ttttatcagc atttgacaga gcaaaatctc
     1921 attcctgctg gtcgtgtact ttatggtgca ggaacgaatt cagaagtaac atactttaat
     1981 tgctacgtta tgccattcat ccatgactct cgtgggggcc tagcaaaaca tcgtcaagaa
     2041 gtgatggaga ttatgagtcg cggtggcggt gtaggaacga acggttcaac gctacggcca
     2101 aaatcggcga tcgtccatgg ggtgaatggg aaatcatcag gttcagtttc gtggctcaat
     2161 gacttagcta atttgacgaa tttagttgaa caaggcggga gtcgtcgggg agcacaaatg
     2221 atcatgttga atgattggca tccagatatt tttgaattca ttatctctaa gattcagaat
     2281 ccggccattt tacaaaattt gatccacact agccaaagtc cgacagtgcg ccaattagct
     2341 agtgaaaaac tgacttttga accactgaca gcaaatgaaa aagaatggtt agaatttttg
     2401 gttgatcatc cgacaaatga tatggaggat aaatttttaa aaacggctca acagaaatta
     2461 aaagcgggtg gtacgtacag tgtgaaagac cctgatttct tgaccggagc caatatctcc
     2521 gtctgtttaa caaaagaatt tatggaagct gtcgaaaccg accaaactta tgagttacgc
     2581 tttccagcaa tcgaagacta tgacgctagt gaaatggctg attatgatca acattggatg
     2641 gaagttggag atgtcagaga atgggctgaa acagggcgta aagtaaaaat acatcaaacg
     2701 atcaaggctc gcgaattgtg ggatttgatt acttattgtg caacgtattc agcagaaccg
     2761 ggaatatttt ttatcgataa tgctaatgag atgaccaatg ctcaagccta cggacaaaag
     2821 gtagttgcaa ccaatccatg tggggaacag ccattgacgc cttatgcggt atgcaatcta
     2881 gcagcaatta atttagccaa tatggttgat aaaaaaacga aagttgtcga ctttgaaaaa
     2941 ttaactgatg tcgtaagagc tagtgttcgt tttcaggaca atgtaattga tgcgacgcct
     3001 tattttttta aaaaaaatga gcagcaagct aaaggtgaac gacgcgttgg acttggggtc
     3061 atgggattgg cagatttatt gatttattgt gaaaaagtct atggatctga agatggaaac
     3121 cgtttagtag atcaagtatt tgaaaaaatt gctattacgg cttatcaaac gtccgtcgaa
     3181 ctagcaaaag aaaaaggaag cttcccattt ttagtaggca aaacagtcga ggaaacaacc
     3241 gcgcttcgtc aacgttttat tgaatcgggt tatatgaaga agatgccgga atcaatccga
     3301 caagcgattc taaaatacgg tattcgtaat tctcatttac taacagtcgc accaacaggg
     3361 tcaacgggta ctatggctgg tgtttcaacc ggactagaac catatttctc tttttcttat
     3421 tttagaagtg gtcgcttagg aaaattcatt gaagttaacg ctgaaatagt cagtgaatat
     3481 ttagaaaaaa atccagagaa aactgcggat aatctaccac cattctttgt cactgccatg
     3541 gagctttcac caaaagatca cgtgagtgtt caatgtacga tccaacgttg ggtagatagc
     3601 tctatctcaa aaacagtcaa tgcacctaag ggttatcaag tgaaacaagt agctgcgatc
     3661 tatgaagatc tgtatcaagg cggtgctaaa ggtggaactg tctatgtgga tggtagcagg
     3721 gattcgcaag tgttgacact tgaagcagat ggcaatgtat ttgaagaacc tgtagtaggg
     3781 gtggcagata atcaaattac tcttgactca ttagaatcat cagaagcaga agaaatatgt
     3841 ccgatttgcc atgagggtag aattgaagaa attggtggtt gcagtacctg cactaactgt
     3901 aaagtacaac taaaatgtgg cttgtaaaag gagctattcg atgaaaattt atactaaaag
     3961 cggcgataaa ggaaatacta gtattattgg tggagaaaga ataaaaaaat ctgacgctcg
     4021 cgtttgtgct tacggtagcg ttgatgaagt aaattcttgg gtcggtaaaa ttatttcaga
     4081 gttggatgat aaaaagtttg agctgttgaa aaaagaacta gttgtattac aaattttact
     4141 ttttgatatt ggaacagact tggctacacc catcgggatt aaagaaatga ttgtagggaa
     4201 agaagaagta actaagattg aaacgctgat tgatttttat caagcaaaag ttccagtcat
     4261 tgaaaaattt atcttacctg gaggacatcc ggtggcatgc gatatgcaaa ttacacgaac
     4321 agttattcgt cgggcagaac gtgaaatcac acgattgatt gtttcagatg aacctatcaa
     4381 tttacatgca tacaaaatta tgaatcgctt atcagattta ttttttgtac ttgcacgtta
     4441 tgtaaatcat gtttatgcta ccaaagagcc attttatgaa cgagcaggga aggtattcca
     4501 ttaagagagg gttttaaact tgaaaaaatt agcaacatta ttaaccgttt ttatcggact
     4561 attcattatg acagggtgcc aatcaaatca aaatgatcaa gcctcttcaa aagcatccac
     4621 tcaagcaaca agcgaggttg ctaaatcaag cgcgctgata aaattgactg atagtgataa
     4681 agagatcact tcaaaaacag tcacttacaa atcaggagaa tcattattgg taattttaaa
     4741 ggctaacttt gaagtaaaag aaagggatgg ctttattact tcaattgatg gtcatgcgca
     4801 agatgagagt caaaataaat actggacatt cactatcaat ggaaaaatgg gtgaaaaagg
     4861 cgcaggtgaa actacattag ataatgatga tcaggtagtt ttcaatttgg gtgtatttaa
     4921 ataaaaatta tcgttcttta cagcaattga gcgataatta agtaagagaa aattattagt
     4981 ttttaaagga atatgttaat tttaaaacac gttcaaacca atcatttcga aggggttgaa
     5041 cgtgtttttt ggttctgttg caaagtttta tctaaatacc tatttagtgt aaaatagctg
     5101 aaactaaaac ataaggagaa ttaccatgtc caattttaaa ggtaaacaat ttgaaaagga
     5161 tattatcatg gtagccgtat gctactattt aagatataac ttaagttatc gtgat
//
LOCUS       DACDLI010000124         5192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDLI010000124 DACDLI010000000
VERSION     DACDLI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.125"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            137..748
                     /locus_tag="IV353_002628"
     CDS             137..748
                     /locus_tag="IV353_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9010796.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IV353_002629"
     CDS             821..1750
                     /locus_tag="IV353_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9010797.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="IV353_002630"
     CDS             1908..2645
                     /locus_tag="IV353_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP9010798.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2693..3871)
                     /locus_tag="IV353_002631"
     CDS             complement(2693..3871)
                     /locus_tag="IV353_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9010799.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3894..3977
                     /locus_tag="IV353_002632"
                     /pseudo
     CDS             3894..3977
                     /locus_tag="IV353_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4098..4499
                     /locus_tag="IV353_002633"
     CDS             4098..4499
                     /locus_tag="IV353_002633"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010800.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5192)
                     /locus_tag="IV353_002634"
     CDS             complement(4706..>5192)
                     /locus_tag="IV353_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9010801.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1642 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta aa
//
LOCUS       DACDLI010000125         4878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDLI010000125 DACDLI010000000
VERSION     DACDLI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.126"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..857)
                     /locus_tag="IV353_002635"
     CDS             complement(<1..857)
                     /locus_tag="IV353_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010802.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDL"
     gene            complement(1065..1991)
                     /gene="glsA"
                     /locus_tag="IV353_002636"
     CDS             complement(1065..1991)
                     /gene="glsA"
                     /locus_tag="IV353_002636"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9010803.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(2014..3441)
                     /locus_tag="IV353_002637"
     CDS             complement(2014..3441)
                     /locus_tag="IV353_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9010804.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3472..4686)
                     /locus_tag="IV353_002638"
     CDS             complement(3472..4686)
                     /locus_tag="IV353_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9010805.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1529 a         1027 c          838 g         1484 t
ORIGIN      
        1 aggtcattga gaacaaaatt tttataaaca acttcgaaat cttttcttaa cagctccatg
       61 ttctcattgg tataatttct actgttgaac aaagaaaaaa tataatagac atcttcttct
      121 tgtaagtcta gaattccttt ttgctcttcc aatccttgac agactaattg aaataacggc
      181 ttcgtttctg cgcttttctt atcttcttct ttcagtccaa ggggaaatgc ctttcttccg
      241 attgcgatct ctattcctcg tatgataaag gacttttcat cttccgaata tcttctttta
      301 aaaaaatgag tctctacata atcaatcaac tcgttacaag cttcttctac atacggattt
      361 ttattttcat agccttccca attgacataa cgtactaaag ctagcaagat gttccgcgag
      421 tcaaattctg ggataatgat ttctttattt ttcaaataat cattttcttt aaaaaaatcc
      481 aatacgattt ttttgattgt atatactttg ctcaaagaaa taaattcaag atcagccagt
      541 tctgtactcg tgaatatacc attaaaataa taacttaaaa actttaaaaa ataagattga
      601 ccgtacagaa actgtgtgag ctcagaaaga gcaataccat gtttgaatac gagtttatac
      661 ttatgcttct ctcttacttg ttcttttata caatattcta gacactcggc tagactttct
      721 agatcgtgaa ggatcgttgg tgtagtcacc cttagagaaa tagctgtttg ttcgatgtct
      781 atttcttgga attcaaataa ttgttcacac agatacactt ttctaaatat atttcgctcg
      841 atgtaatttt ccagcattcc tgatacacgc ctccagtttt tcttatgatt ttatttaatc
      901 atatctgtaa atcgtatttt tgtttccttt ttatttagaa aagccctttc aaaaaccaat
      961 aaaaaaagaa actgggacaa aagtcaaaca agctcacaat ttcagcctca ttgtaggtag
     1021 ctttgagccg tttggacttt taccccagcc tctttcaaga atggttattc aaagatgtca
     1081 gcgtctagtt ctttgactac atctttcaac aattggatac cagctgcgct gttaccaaat
     1141 ggatctagtg ccgggctgaa gacacctatg ccgtaacgat ttggcacagc tgccatcaac
     1201 ccgccgccta caccgctttt tgcaggtaca ccaacatgga cggaaaactc tcctgattca
     1261 tcatacaaac cagcagttgt cataattgat ttgacgattg tagcactttc ttcagtaatg
     1321 atttgtttgc cattccaagg agcaattcct ttatttgcca gcacagcagc taattttgct
     1381 atgcctgtag cggtgacatt gagggagcat tgtctgaaat aagcatcgag tatctccggt
     1441 acgtctcctt cgatcatttg attacctttc atatagtaag caagagagcg attgatatca
     1501 cctgttcttg attcagaatg atagatttct tcattcaatg tgatattagg atcatcacag
     1561 atctctttca taaatgacaa gattcgctca aatttttcat ctgcatcttt accggcaatc
     1621 aacgaggttg ttgcaatcgc tcctgcatta acaaaaggat tcattggatg tttgcgatga
     1681 ttgatttcca tattcatgat agaattgaag gcaaaaccgg ttggttccgt gttgattgtt
     1741 tgaaagactt tttctattcc gttgtctttg attgctaata acaatacggg aacttttgac
     1801 atgctttcga tggcaaagcg cacatcggcg tctcctccct caatcagttg ttttgtatcc
     1861 aaatcataaa tcgccatacc taattgtttg ggatccactt cagctaatgc tggaatgtaa
     1921 tccgccactt gtcctgtttg ataatagtgg aaattttttc caatcaattg ttctaattct
     1981 tttacttcca tttgaataac cagctttcat tttttatttt tctgcatgct ggaatttatg
     2041 acggcatgcg tagatcgtaa atggaataac taagacgata agataagaaa caatcagaat
     2101 cgtttcataa atcccatgct gtccagctgc gatactagca ggtggaacaa atgagataca
     2161 aaaagcaaaa agtgaagtca aaagacctaa acaagcaacg attactttta caacttttcc
     2221 gcctggaacc tgataggctc tttcaagact gtcatcttta aaaaccagct tcaaatagcc
     2281 cgcgaaaaac ataaagtaca acatcaggta gattacaaca gtcaatgaca tcgctgcaag
     2341 aaatgaaaga ttattccccc cgccgcttaa tgtaaagacg gctgcccaga ttgttgcaac
     2401 gatcccattt gcgatcatca aatgagcagg aacaccgtgt gtgttttctt ttgcaaaata
     2461 ttcaggaagt aatccttctt ctgctgctac gtgaagtgct tttgaaggag aggttaccca
     2521 agaagcaatt tctccgatga cacctaaaca aacaagaatc gcaagaattt tcgcaatcca
     2581 tgcaagattt ggatcaatgt gtcttaatag gaattctaac gattgaatca cccctgtatt
     2641 aagagacaaa ccatgttgag gtactgtagt agcgacggtc aatccgccaa aagtatctaa
     2701 aatgattgcc agaataacca atacaaacat tgctaatgga taatcctttt tagggttttc
     2761 catttcattt gcatgactgg cagatgtttc tacgcccata tatgctaaaa taaaggaaac
     2821 aaaaacaacc agcgtaggca atttcgaaaa atcaggaatc aatgttttca tagacagtat
     2881 cgtttgtagt ggagcgcctg aagcgacata atggatccct aagccaaaca agactaatgc
     2941 tggcaacaag ataccagcta caaaaccgat ccgagcaatc aatgaagtgt atttggttcc
     3001 ttttaactgt gagagtaaaa tagcccagaa aataatcaat aggattgcca atttcagata
     3061 tttattgttt tgtatagcac caatacccgt tgcataggat aatgcaccag ttaagaaata
     3121 cagcatggtc aagtagccaa ctgttatctc aaaccattgg aaaaagattg cggcaaaacc
     3181 gaatcgtttt cccaatgtgc gactgaccca agtatagaca ccgccttttt cccatccttt
     3241 caccgttgcc atctctgctg cgcacaatgc cacagggata aaccatagca aaccgcctaa
     3301 taacaagaaa aatactaagc tgaagcctga ggtcgcgaat gtgggatatt catataatga
     3361 aacgaccatt gagatggtca tagccaaaaa gccaaagaac gttaattttt ttgcaggaat
     3421 tttcgttttg ctttgttgca tgaatcttcc tcttttctat ttgaatttgt ttcaattgag
     3481 tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg tagtagtgat
     3541 cgttgtaaag tgcatcgccg attttaaaat cactgttgtc agcacatagg catacacggc
     3601 agtaatgatg acaccaatca cttgaatccc taagaagtac cagctgcttg gattcgttag
     3661 taaatggctg ttagatgcaa agatccctat cagaatcgta cctgtgaatc cgcccatgcc
     3721 atgaacaccc cacacatcaa gtgcatcatc ccattgtttc tttttacgaa aatcaacagc
     3781 agcatgacag acgatacccg caatgatccc aatcagcatg gcactttttg cttctacata
     3841 accggcacaa ggcgtaatgg ctgccaatcc agcaacggat cctgttagta cgtcgacaaa
     3901 atcaaagcca tttcctttta cttttgcaat gatcagccaa gtaatcatcg caaatgcaag
     3961 agcgatgaat gttgaaccaa atgcattgac tgcttgttca tctgctcgaa gtgcaccacc
     4021 ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta ccgatagcag ctgccataag
     4081 actgctatgc tgacttcttt ctcctaaagg aatctttctt tgaccaagtg tgaggataca
     4141 tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga aatctctaaa
     4201 tcccatctga tccaaaaatc cgccacccca tatccagtgg caaacaggga aataaatcag
     4261 cagattccaa aaaataacaa gtataatata gccttttaaa ttcaatctcc ctgcaaaagc
     4321 tcctgtcatc aaaggaacgg taatcacgat aaacatcagt tggtaaagaa agaacattaa
     4381 aaatggaatc gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc tcatcaaaaa
     4441 gaaatctgcc gggtttccaa taacaccgcc gatatcccgt ccaaaggcca atccgaatcc
     4501 accaaatacc cacataatcg ttgttatccc aacggagaga aaagattgag acatgatcgt
     4561 tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag gagtcatcag
     4621 gcaaaccatt gctgtacata aaatcatgaa tgctgtatct cctgtattga taggtaatgt
     4681 attcattttt ttcgcctcct ttctacgatt acgagtataa aagaaggtgt agttgttttt
     4741 ttcttctgtt tttttgaatt aatacttgca tttataaaag acagacaatt aataaaagct
     4801 tgatgtgttg tgctcttttt cgtttttcac acaaataaag acaaaaaata aacggtcttc
     4861 tttttataga aaaccgtt
//
LOCUS       DACDLI010000126         4805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDLI010000126 DACDLI010000000
VERSION     DACDLI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.127"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IV353_002639"
     CDS             <1..411
                     /locus_tag="IV353_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010806.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="IV353_002640"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="IV353_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9010807.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="IV353_002641"
     CDS             2446..3708
                     /locus_tag="IV353_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP9010808.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4805
                     /locus_tag="IV353_002642"
     CDS             3953..>4805
                     /locus_tag="IV353_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP9010809.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1658 a          666 c          981 g         1500 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaactat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaa
//
LOCUS       DACDLI010000127         4740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDLI010000127 DACDLI010000000
VERSION     DACDLI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.129"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1117)
                     /locus_tag="IV353_002643"
     CDS             complement(<1..1117)
                     /locus_tag="IV353_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9010810.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENAQTVDKV"
     gene            complement(1247..2623)
                     /locus_tag="IV353_002644"
     CDS             complement(1247..2623)
                     /locus_tag="IV353_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9010811.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2623..3279)
                     /locus_tag="IV353_002645"
     CDS             complement(2623..3279)
                     /locus_tag="IV353_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9010812.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3289..4566)
                     /locus_tag="IV353_002646"
     CDS             complement(3289..4566)
                     /locus_tag="IV353_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9010813.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4585..>4740)
                     /locus_tag="IV353_002647"
     CDS             complement(4585..>4740)
                     /locus_tag="IV353_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP9010814.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1478 a          863 c          574 g         1825 t
ORIGIN      
        1 ggactttgtc tacagtctgc gcattctcat tagagaacgc tccttttttt gtgacttttc
       61 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      121 taccataatc aaatagttgt tcaacatgag aaaagtttcg tttgcataca tctactgcac
      181 cagaataatg agaccaccga ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag
      241 aagaagcact gtagaatttt ttcatgtctc gacagacttc cttataatcc ttacttggga
      301 ggtatcgtaa cgcatttcga acaagatgaa caatacagcg ctgcacaatg acagaaggaa
      361 aaattgcttt tgcgccttct ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat
      421 cttctacacc acgtactttc agttcatcaa atacttgcat ccagcgattt ttagattctg
      481 tttgattgag ccataggcct aaaatctcct tgtttccttt aagatcatag ccaagaatgg
      541 tgtacacagc atattcttta gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa
      601 cgaatagaaa tgcataacac ttggctaatg gccgagcttg ccattcttcc aattcaggaa
      661 ggacggcatc cgtgatatca gaaatcattt cgtgggaaat atcaaagccg taaatatctt
      721 caacggttgc ggcgatatcg cattgactca ttcctcgtgc atacatggaa agaaccttac
      781 cttcgatgtc ggagacatct cgttttctat taggaatcaa ctcaggttca aaagaagcct
      841 cccggtctct gggaacatca atgtttactt taccaaagct cgttttaagc gtcttattgc
      901 cataaccgtt tcgtcgattg tcatgtttct ttggctcttt agaatgggcg tcatacccta
      961 aatggttatt caattctcct tgaagcattt tttcaaataa gggaccaaac acatctttta
     1021 aaacatcttg catatcctct acagattcgg gttgataagt attcaaaatg gattcagcta
     1081 acttctcagc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1141 ttttagaaaa ataaaaaccg tgaagcaacc acccgcctag gattgatggt tacttacacg
     1201 gtttatatta cactctcatt tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa
     1261 ttaataattc ttttggattc cttcttaata tattgaatga agaaataatc aatgatacaa
     1321 gaagtactaa actcataaat ataactacat aaatcatgaa tttaggtaat acattgatat
     1381 ctaaacttga taatgtcttg ttaaatcctt cagcttctgc cccaccacct aattggctag
     1441 atgcagattg tttagctatt tgtttagcta tatcaccagt aaccttattc aatatattat
     1501 tcccaagctt gtcagctgta tattgagcta agaaatagga accaacgaat gcggggattg
     1561 atataaatac catctcaatt aggaattgac caaaaatttc taattttgat atacctaatg
     1621 atagtaatac tgctatttct ttctttctag cattcatcca taagaataat aataatgaaa
     1681 ctacaactcc tgcaaatatt aatgagccaa aaaataattt atttgaaatt gagtaaatac
     1741 ctgatataga ttgttgtaat gcagggtaat ttgatgaact tttaatcaaa ttatattctc
     1801 tccaatttat atctaatttc ccaagatctt ttattacact atcaagattc ttatcacctt
     1861 ttacaaagaa tgttgcatct tggtatacag ctgtatcttc ggtattacca taaactttag
     1921 cagctgtatg aatatcagtg attaatgtat tttcgtatag ttcttgtgct gcacttactc
     1981 caccgctatt atgcccatcg aatagacctt taatttctac ttctactgtt tcatctgcac
     2041 ctttctcatt atcagcatca aataaattag attttatttt tatcttatct ccaactttaa
     2101 ggttattttt ttcagctaaa tctttatgca ttaagatttt atttttatct tcattttcta
     2161 aatgctttcc ttctactaat ttatatgctt ctgatacaaa ttttgtttct tttgttgagt
     2221 cgttaacccc agttaacata actgttcttt taaaattctt tgctctttca ggtgattgat
     2281 tcgcaagagt ctcttgagtt tcaataatat cataatcaac taaatctgca acactgttta
     2341 ttctttttac ataggagtct atactatctg tttgagatat ctttttaata tcttcacctt
     2401 ttacatttcc cccacctcta ggtgttcctg gatttacttg tctatttatc tccatagaaa
     2461 aactatttgt tatattagca aatgtttctt ttgaagctct gtccgtagca tctttaatag
     2521 ataaactaat gagacttaaa gttgacatag ataaaataac taatatgata attaatgatt
     2581 ttaaactttt tctagttaca taagcaaatg cattttttat cattattcaa cctccaaatt
     2641 cattttattt acttttttca actttttacc acttagttct aaaatgatat ccgcagaatc
     2701 tgctacttcc ttactatgtg ttacaactat tacacattta tttctatctt tagctaatgt
     2761 ctttaatata ttaattattt ctccagcagt aacactgtct agattaccag taggctcatc
     2821 agctagtatt attggagcat ctgataccaa tgctctagca atagctactc tttgttgctg
     2881 tccaccagat aatttcataa catttctttt tatttgtttt ttatctaaac ctagttcaaa
     2941 caagatactt tcatctgctg atttatttac taatctaata ttttcaattg gtgataaata
     3001 atctattaaa ttatagtttt gaaatactaa agatatatta ttttttctat gattactata
     3061 tcctttattt tgtatatctt cattcttaaa caatattttt cctgtttgag gtttatcaag
     3121 tccagcaagt aaggaaagaa gtgtagattt tcctgttcct gactttccta ctatcgcata
     3181 aaacttccca agttcaaatt tttgatttac tcctgacaaa actttttctt tagaatttgc
     3241 ataactatat gctacattct ttatttctaa tatatccatt attttctcct aacttatttt
     3301 tgataagatt tctttaggct tcttgattaa cattaatgaa gaagcaaata caactgataa
     3361 aacaataata cttattaata ttaaataact ttgtccaagt gttgttatat ttgacataaa
     3421 actactatta tttattaaac ttccaccgga aatcattgaa tcctctgagt taatgaatcc
     3481 atctacaatt acttttagta atacatttcc taaaaataag gaagatatta tacttggtat
     3541 cgatatgaat attaactcga atataaattg cattataatt tgtatcttag atgttccaat
     3601 agataagaat atacctattt cataaattct ttcccttaac catagaatca agattaatga
     3661 aagaacaacc atcccaccta acataataga ataagtcatt acttttatta tatattttat
     3721 tccactcact gactctaaag actcttcaaa cgcattagaa tctttttcaa caaaatactt
     3781 tgactcatca attttaagct ctttcaattt gtttaaggct aagtctgtag attctgcact
     3841 accagaatac attaaaattt tatttgcaat ttcattattt tctgaattgt ttaatatctc
     3901 ttggcttgtt gaataatcta caaacaccat attttcacta aaatcagaag ataatcctgt
     3961 atatgtttcc tgttttttac cagaaaagat ccctataatt ttaaatttat gactttttat
     4021 ttttccactt ttttcaatat ctagtaattc aagatcaact tcatcaccta attttagatt
     4081 gttttgttta gcaaattctt catgaacaat aattgaattc ttatcatttt cttctatatt
     4141 tttaccttct ttaattgtaa atactccact actaaataaa atatttcttt tagtattatt
     4201 tgtagcttcg agtgaaacaa cattcttaaa ttcgtcagat aaatcttctc tatttattct
     4261 ttgttctccg ctaactactt tagcatcttt tagttttgct aatccatcat attgaattat
     4321 tttttcttca atttctttta atttttcaat atctttaaat tgattaacat taaaatattt
     4381 accatctttt tttgttattg atattgaaga attagaactt tcatataaag ccttttctat
     4441 ttcattgctt gatttcatta ttgttaaaca agaatacaag caagaaagaa ctatagttaa
     4501 tatgataaaa attattaaag ttctattttt ctttcttgta atatatgcta tagcattttt
     4561 tatcactttc ctaactactc ctctctattt tcttactcca taactaattg catccactgg
     4621 acaagcattt ttacatcgtc cacatctgat acactccaga tgattgcaat tctcaactga
     4681 atctttattc atttggcaaa cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DACDLI010000128         4316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDLI010000128 DACDLI010000000
VERSION     DACDLI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.130"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..1108
                     /locus_tag="IV353_002648"
     CDS             134..1108
                     /locus_tag="IV353_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9010815.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="IV353_002649"
     CDS             1090..1551
                     /locus_tag="IV353_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9010816.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="IV353_002650"
     CDS             1601..2065
                     /locus_tag="IV353_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9010817.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="IV353_002651"
     CDS             2079..2828
                     /locus_tag="IV353_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9010818.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="IV353_002652"
     CDS             2828..3658
                     /locus_tag="IV353_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9010819.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="IV353_002653"
                     /pseudo
     CDS             3658..4305
                     /locus_tag="IV353_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
BASE COUNT         1484 a          556 c          821 g         1455 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa ttttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtatcgtaaa gttatataac tttataatac ctattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtattaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgtatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttcataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgactcc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgtaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag tttttt
//
LOCUS       DACDLI010000129         4219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDLI010000129 DACDLI010000000
VERSION     DACDLI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.131"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(394..711)
                     /locus_tag="IV353_002654"
     CDS             complement(394..711)
                     /locus_tag="IV353_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9010820.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTIHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(712..966)
                     /locus_tag="IV353_002655"
     CDS             complement(712..966)
                     /locus_tag="IV353_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9010821.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTVQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1226..1444)
                     /locus_tag="IV353_002656"
                     /pseudo
     CDS             complement(1226..1444)
                     /locus_tag="IV353_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1710..1904)
                     /locus_tag="IV353_002657"
     CDS             complement(1710..1904)
                     /locus_tag="IV353_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010822.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(2474..3664)
                     /locus_tag="IV353_002658"
     CDS             complement(2474..3664)
                     /locus_tag="IV353_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9010823.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKMELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDSYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVNNRKEVCDDFKMV
                     YQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT         1292 a          775 c          686 g         1466 t
ORIGIN      
        1 tatattggac ttcctctttt aaaacacaaa aataaatact accttattta ggtagtattt
       61 attaaaagtt acgtcaattt tgattaaaaa tatactactt gaacctctca tgagtaaagt
      121 cacgaaattt ttgggaatag agcgtacttg atatcttatt ttttcactaa tagcagtgtc
      181 tcttattcac caagcgatcc ccgtagttcc tacggttctt aatttttctc tagaccaatg
      241 attgtatctt tagaccttcg gccataacat ggagcaaaat aaaaaggtac taagtcttat
      301 atatcaatct tcgagtaacc gacggttaac aaccatatcg agcggcatta atcacccacc
      361 tgtagaggat gagcgacttt tgcctaatta ttgttaaaag cctagtttca tttgtttaga
      421 ttggaaaata cgtcctacaa gtatctcttt tttttcatgg tcgatccgat aaaataaggc
      481 atagcttttg ccgatcacga tccgatatgt tgggatatcc attccataga tttcggaaac
      541 ttcctcatgt atagttggaa aatttttggt tagttctaaa gccttctgga tcgcacctac
      601 aaatcttttt atgctggctg gagaaagagc aagctcattt tcccattctt ctattatcgt
      661 ttcaaggctg gtctggaaac ttttagaata aactagcgtg tactctttca tctattcact
      721 ccagccgtat tttccgaaag cttttaccat ttcaatttct gaaactgtta aacctttctc
      781 aaagtcttgg aaactttctt caatcaattt gcgatcgtgt tcttttgcta tttgagtagt
      841 atctaatatg aataagttgc cttcttgttt ggcttggaca gtgtctccgc tttttaatcc
      901 aagagagctt ggaaggatca ctcccacact attgccgatc tttctaacct ttagttcttt
      961 tcccatgtta tcacactcct tttagttttt gatgaataaa ttataacatg ttataacaac
     1021 aattgccaac ggtgtttctt aagggattcc actccatatt attattggac aaaataagcc
     1081 acttagaacg ctgaaaacat atacaaacga tatcaaaata cgagtgaact actcaccata
     1141 cacttccgtt aacatccatt taagtaggta tttttattgt atatagattt aacatatgtc
     1201 ggtcggttga ttgatatagc taaagttata actcaaccga agccgattca tctacacgaa
     1261 taaagttgcg agtgttctcg gctaattcat aataattgtt atgtgttcca tatagcaata
     1321 ctggaataac tttattttct tgaatctcat aagcaactct atacacggtg accgcatact
     1381 taaatccatt agaccaatta cctgataaac ctccagtttt ttgctcttac ttaaatggat
     1441 caatagcaat ttcatcacaa acctatagag gacgagtgac ttctgcctaa ttacgttaaa
     1501 aaatattgaa agtaactcta tgtagcaaaa aatatctaga atttcatttc ctcaaaactg
     1561 atcgatattg tatctaaaaa tatatactac ctaaaatagg tagtatatat taaaatatgt
     1621 gcttttgtta gaaaaatcaa tacataaatt atcatatctg tatatgtcat ttaggtattt
     1681 cagttatgtt ataatagttt tgctgtctgc taccccggcc taatctggca aggggaggag
     1741 gagatataat gaaatacctt tttgattttg tttttgcaat caagccattt gcagatgtag
     1801 tgtgcagtta cctctgcaaa tggcttgatt gcaaaattgc caaaaaagac agtaagccta
     1861 ccagttaatt ctataatata gaaacgtaaa aaaaacccca tcagcctgca cgctgatggg
     1921 gtttggtgat ataatgaaat acctctttgt tactcatagt tataccataa ttagtcacga
     1981 atgcaaccaa tagaaaaaac tacttagaac ggtagatcat cgctaataac aatgcattgt
     2041 ccaccaaatg gattttgtga agcattaaat acattgttat taattgtcgc tgcttttaac
     2101 taggcctctc cccttctatt ataaaagaag atatatatca tttcttaatt acctactacc
     2161 ttaaaccaaa atttaagtac tacctaattt aagtagtact taaacaatct aaaaaatagt
     2221 tatcccaatt gttatgcttt tataatacga ttatttgtaa caaaatgaat attatataat
     2281 taaacactac catatttagg tagtgtttaa ttattagcaa aacaaaaaat atttattcta
     2341 ttctcttaac ttcaactaag ttatgctgca cacctttata taaatcatat gcaactcatt
     2401 agctagacaa actacaagca tggggagcgt caataatttt gtgtaaatgg ttcctcccta
     2461 ctgtagattg tttctaattc tcaatcagtt ggcttagcat ttgttcaagt tcgccttcgg
     2521 cctgttgaaa gcctttatga ctgcgaccta ggaatttttg gttgtaagta tcaaaagacg
     2581 agactaagaa acgttccatc gactcttcgt tttggaactg ttccttgcgg tggctgtatt
     2641 tcttgatttg cttattaaag gattcaatca agttcgtaga ataaatactc ttgcgtatgg
     2701 ccaaggggaa atcatagaaa gtgagcaagt gatcgttcga aagaatcgat tcaaccactt
     2761 ttggatagct ggttttccat tttttcgcaa aagcaccacg tgcttccaac gccacctctt
     2821 tagacgaggc ttgatacacc attttaaaat catcacagac ttccttacga ttattgacac
     2881 gcactttgtg actgatatta cgggaaacgt gtacacaaca atgttgaaaa cgagctttgg
     2941 gatagaaccg actaatcgct cctaccatcc cctttaagcc atccgtgatg aacaggagga
     3001 catttttcaa accgcgctct tgaaggtcca ataaaatttc ctcccaaatc gtgatggatt
     3061 cagtcggtgc aatcgcatag ctcaatactt cctttgatcc gtccgggcga atgccaactg
     3121 cgatatgaat agcttccttg gcgacggttt tccgctttaa cggaatatac gttgcgtcca
     3181 tataaatagc agcataactg tcatgaagct cccgcccttt aaacgccgtt acctcttctg
     3241 taaatgattt tgttatattg gacatggttt ggggcgtgta gtgatgccca tacattttct
     3301 caatcaagtc tgcgatttcc gacatggtaa taccttttcg gaagaggtga atgacggtct
     3361 cctctaaggt atcattcgtc cgtctataag cgggaacagt ctgttgcttg aactcgccgt
     3421 tgcggtcgcg cggaatctgg agatgaagtt ccccgtactc ggtcttgacc gtacggtcat
     3481 aggagccgtt acgagaatta cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga
     3541 ggaaagccgt taactccatt ttgagaagcg tattgacagc tttttccaaa tggacacgga
     3601 ataattcatt caaatcgcct ttgttcgcta gagtctttag aatttctgta gtaaaatcgt
     3661 tcatatggaa gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac
     3721 ttcctttttt tctatttaca caaaatattt tacactctcc aagcatgtag ttttatctgt
     3781 taattatgta ctacctgaat aaagtagttt ataattaaca agtatatata tttaaaaatt
     3841 taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat taagaactac
     3901 ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca atttggtagt
     3961 atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag gtagtatcta
     4021 atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact ttaaagtaat
     4081 ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg gtagtacata
     4141 attattatat atatctgaaa ttggtagtac ttattgtatt ttttagtagt atataatcta
     4201 ttgttttcta gcctaaagc
//
LOCUS       DACDLI010000130         4147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDLI010000130 DACDLI010000000
VERSION     DACDLI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.132"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..388
                     /locus_tag="IV353_002659"
     CDS             80..388
                     /locus_tag="IV353_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010824.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            392..754
                     /locus_tag="IV353_002660"
     CDS             392..754
                     /locus_tag="IV353_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010825.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            975..2381
                     /locus_tag="IV353_002661"
     CDS             975..2381
                     /locus_tag="IV353_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9010826.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSENEIEW"
     gene            2828..3802
                     /locus_tag="IV353_002662"
     CDS             2828..3802
                     /locus_tag="IV353_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9010827.1"
                     /translation="MSKRGTIVNEWEKLNRISNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     HEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIACDIVDIPDDFVSQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRESFAESADDLKRELDTWLQGLDVTEVGAE"
     gene            3799..>4147
                     /locus_tag="IV353_002663"
     CDS             3799..>4147
                     /locus_tag="IV353_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010828.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQ"
BASE COUNT         1446 a          603 c          928 g         1170 t
ORIGIN      
        1 aaaaaaacga tcagttgtta ctagaaaatc aagaattaaa aaatcgaaaa tggtggcaat
       61 tctggaagtg aggtgtgaca tggataaaat tgatttgaga gaagaaacag tcgatagaat
      121 tgaattgatg tatgaacggt tagctgatta tttgaatttg ccggatgaag ataattatag
      181 tgaaatgctt gaacgaatta tggatgttct caactcggtt atcaatgctg atttagcaaa
      241 atcttcagtt ttgactaatg aatttgttca taagcattta gcgcaaacaa ctgatgaatc
      301 agaaaaagaa ttttggttga aattaatgag tcatgttatg cagttaaacc aaatttccgt
      361 tattaattcc aaagaaaagg agatgtagtt tatgggaatg actttagcag atattaaagc
      421 gcaagaagaa aaattaaaaa aagaacaagc gaaacttcgc caacagaaaa aacgcttgat
      481 tgagaaagaa aatgctgcaa tgggcaaggc tcttcgcaag cgttttgggt ttgaaacttt
      541 tgctgatttt gagcggtggc tatcgtcgat ttctgacgat cagcaacacg aaaaatcagt
      601 ggataatgag tggcttgctg atgaaaaatc agcgccaagc gaacgagatt atcccgatga
      661 atctgacggc ttattcggag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
      721 tacgtcaata tttaatgacg tgaatttacg ataagcgatt attttgcgtg aagcaaaata
      781 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
      841 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
      901 tgttactctt tgacaaagag taacagaaag gtttacactc tttttattag cgacagccga
      961 aggagggttt taacttgggg ttcagtattt cgttcaaaaa gggaacaaag aatacaagca
     1021 tttctcataa taatcgtgag ttaactgata gccaaaaaat caatgactgg cataagcata
     1081 ttgatttcac aaaatcggat gaaaatatct atttagaaca aacaaatatt cgtgaaaaat
     1141 atgaagaatt attcggtgaa gccgtagaaa aatataatgc aaagcagaaa agagccgata
     1201 gaaaaatcga ggactattta gcaaaggttc gcaaggataa aaaattggaa ccacagcgag
     1261 aatttatcgt gcaaattggt actcttgatg attttcgtac tactcgtgat gatggttctt
     1321 ctactggcat atcggaacaa caagcagaac aaaatcgagt gattgccaac aaaattttag
     1381 ttgagtattt caaagaattt aaagaacgga atcctaattt atccgtctac aatgctgtta
     1441 ttcataatga cgaagtaagt ccgcacctgc atttgaatat tgttcctgtt gctgaagggt
     1501 ataaacgggg tgttcaaaag caaccaagtt ttaacaaagc cttgcaacag cagggtttac
     1561 gagcagataa agagaatggt cgagtgattt ggaataattt taggaatcag gaagttgagt
     1621 caattgaacg tttgatggca gattttggtt gggagcgtga gttagtcggt actaacaaaa
     1681 tcaaagatgt tcacgaatat aaagaaatca tgagcgagat ttcagaattg cgtcaaacgg
     1741 ccgtaaaaga gcgtgagagc gtttctgatg agttgaggta tatttctagg caaagagaag
     1801 aaaatgagcg agaaaaggct ctggtggctt ctgagagagt gaagattgaa gaagagaaga
     1861 aaaatatgct gaatgtaaaa aaagagatgg cggtcatttt ccaaaaagat atggttcctt
     1921 tagaaagttt caagaggctt cctgatggtt cgtatcattt atctgggaat gattttgttg
     1981 atttgtatca acgagctacg agagcaacaa atgccaagag aaatgctgat aacatgagtg
     2041 agcaatattt acagatgctt gagcaaaata cagaattaaa tgagtatgta gctacattgg
     2101 aagaagagcg tcatttgtct ggtagaaaac aattagcaga tttacaagtg gaaaataaaa
     2161 agctgaaaga agaaaatcgc tctttacgcc agcgtttaga gcgattaaaa aacgcatttc
     2221 aaaaatcgat cactcgtttg tctatccgtt ttggtcttgc agaaaaggat atggggcttt
     2281 ctgaggaatt agaagtatta gagcaggaga aaaccttatt ttatcgtcaa aacgtctcac
     2341 agcataaatt agagcgttct gagaatgaga ttgagtggta gccacgatgt gcagtcaacg
     2401 gtaccggagg cggtttgtgc cgaggataag gagttgacgg ctaaaccact agcctattaa
     2461 gctgacattt gctaaaaaat ggccagtcca ttttttggcc aacctagcgt tagcgaagtt
     2521 tgggggcgcc cttttcaaag ggtgcggttt tattttttgg atatttgcat agctaaagag
     2581 aagtgctatt attgagttag caaacaaatg aactgaaaaa agagtaaaaa aatagtcgag
     2641 caacgctacc aacgttctcg acctttacgg aaactaatcg cactagctcc gtaacttctt
     2701 aatcattata cgaatatttt tagatttctt caagactaga gattttttgg taatgaattt
     2761 ttaagagtta cgtgaacttt tgaaaattct ttccagaagt ctctttttct ttgaggagga
     2821 acaaaatttg agtaaacgtg gcacgatcgt gaatgagtgg gaaaaactta accgcatttc
     2881 taacaggcgg ttaaagtatc aaagtttgac ccttccctta aaaatgcagt ggtcaattga
     2941 ccgaattacc atagtcggaa aactgaaaga aaatatttat tatcacactc caaatgatgt
     3001 gttgatttta aattttgaac agcttatgcg tttgaacgaa ggcaatggtt atttaaaagc
     3061 tgttgggaat aatgggtggc aattattaga ccaacacgag gaaaatatag cttatattga
     3121 gattcttaaa tttcatgaag gaaaagggcg gattgacttt aatcctaata aaatcaatca
     3181 atttttatca ggttccatga aaaatttcat tcatgattta tttctagaac ctcatttttc
     3241 acgtgcggat attgcttgcg atattgtaga tattcccgat gactttgtta gtcagtatcg
     3301 tgtagttgat cctgtgtctt ttaaaccaat ttatgggcgt tctggtaagt tagaaacggc
     3361 atattggggt agtcgtgcca gtgaacgaca aattcgaatg tacaataaaa aattggaaca
     3421 agaaagaaaa cgtaaaattg ttccgccgga aataacgaca tggtggcgac tagaattgca
     3481 gttacgaagg ggaaaagcaa ctgattggca tgagatggtt catgaaagtt tggatagctt
     3541 tgctagtcca cattattttc cgccaaatac taaagttact gaacggatta tggtgaaagg
     3601 tttgctttat gatcattctg agtggggaaa tctagctaag aatacaaaga aaaaatacag
     3661 ggatttgttg aagcaagaaa gtcaaaatga tgaattgacg aatcatttgc gagagtcatt
     3721 tgcggaatcg gcagacgatt tgaaacgaga attagataca tggctccaag gacttgatgt
     3781 aacggaagta ggtgctgaat gagtaatcca gtagaaaaaa tgaccataaa aaatcttgct
     3841 gacgaattgg gtgtatcgaa aaatacagtg cgcaatcaac tggtcaattc agggtttgac
     3901 cttgctcagt ataaagaaaa tggcattatc atgctagata aagagttgat tggtgttgtt
     3961 cggtcaattt atcggtcaaa aagtgatcaa ttgactggtc aattaccggt caataacaca
     4021 ggcagtgatt ttcctttaga aaaagagaat gagtttttgc gtgatcaatt agctgagaag
     4081 gacaaacata tttctgaatt aacagaaatg attcgtgatc aacaaaaatt gaccctgctt
     4141 cagcaac
//
LOCUS       DACDLI010000131         3912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDLI010000131 DACDLI010000000
VERSION     DACDLI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.133"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            93..1019
                     /gene="cysK"
                     /locus_tag="IV353_002664"
     CDS             93..1019
                     /gene="cysK"
                     /locus_tag="IV353_002664"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9010829.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1255..1701
                     /locus_tag="IV353_002665"
     CDS             1255..1701
                     /locus_tag="IV353_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9010830.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            1974..3326
                     /locus_tag="IV353_002666"
     CDS             1974..3326
                     /locus_tag="IV353_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9010831.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3497..3784
                     /locus_tag="IV353_002667"
     CDS             3497..3784
                     /locus_tag="IV353_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010832.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1367 a          630 c          820 g         1095 t
ORIGIN      
        1 ttttttttgc tagtcaaaga cgaatcactc gccataaatg aacagattgt attatgataa
       61 gtaagaagag caaagacaag gaggaattga agatgacagc tatctataac tcagtcaccg
      121 aattgattgg caaaacgcca atcgtcaaat taaataaaat cgtaccagaa gattcagcag
      181 atgtttttgt aaagcttgag ttttttaatc caggaggaag tgtaaaagat cgtattgctt
      241 taagtatgat agaaaaagca gaacacgatg gtctactgaa accaggtgat actatcattg
      301 aacctacttc tggaaataca ggaatcggat tgtcgatggt aggagttgca aaaggatata
      361 aagtaatcat cgtgatgcct gagacgatga gtatcgaaag acgtttattg atgaaagggt
      421 acggcgcaga gttgattttg actcctggtg cggatggtat ttctggatca atcagagaag
      481 cagaacgttt ggcaaaagaa aacggctact ttttaccatt acaatttgaa aatgaagcaa
      541 atcctcttgt gcatgagaag acgactggtc ctgagataca tcaagcattt ggagtaaatg
      601 gcttggacgc gtttgttgca ggtatcggta caggaggaac gatcactggc gctggccgag
      661 aattaaaacg tgtgtatccg aaaatcgaat taatcggggt agaaccagca gaatctgcta
      721 ttttagaagg gaaagaagcg ggacctcaca aaatccaagg aatcggtaca ggttttgttc
      781 ctaaaacact ggatacttct gtgtatgata aagtcctttc tattagtgga gacgaagcga
      841 tggaaacagc tagagaagtt gggcgaaaag aagggatttt agtaggcatc tcatctggcg
      901 ctgcaattgc tgccgcgcta aaagtcgcaa aagaattagg aaaaggcaag aaggtattag
      961 ctgtggttcc cgacaatggt gaacgttatt tatctacagc cctttaccaa gagatataag
     1021 gcacttggtt tttctccaga taggtttatg taaaaaactt ttaaagatga aaagaacaca
     1081 caaaaattta tgatgagaaa aattatttga atagagacgt agaatagtga acgcgactgt
     1141 tttacgtctt ttttcattaa aatacaataa aattgaattt tcttttcatg ttaagtggat
     1201 tttaaaatgt aaaaatagta tgataagacg agaataacaa cgtggggtgt ttacatggaa
     1261 aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag agaacattcg aatcacacct
     1321 caacggtacg cggttttaga atatttgatt gaacatcatt cccatccaac ggctgatgag
     1381 atttataaag cacttgaaca tcggtttcca aatatgagtg ttgcgactgt ttacaataac
     1441 cttcgcttat ttacttcgat tggatttgtt caagaaatga agtacggcga tgcttctagc
     1501 cgatttgatt tcagttcaaa gcgtcattat catgtaattt gtgaaaactg cggtaagatt
     1561 gttgattttc attacccagg gcttgaagat atagaaatgg ctgcaagcag actgactgat
     1621 tttgagatta atgaacatcg gttggaattg tatggactct gtccggattg taaagagtca
     1681 gcaaaaaagg cgcaaatgta aaaacagttt ctacaagtgt tatacctaaa aaaagctagc
     1741 tgtgtaaaaa aaacgctagc ttttttattt ttttgtcttt tattcacaat cttagatata
     1801 aaagtgaata ttattaaaat aagaatcttg tagatattgg gttcttatgc ttgaaaagga
     1861 atgaaactac aaacaaacct ttgtgaattc acaagcatta aactgttgta tccgttttat
     1921 ttctgttata gtagttttgt cgaaggacaa aatgttaatc ggagggacca taaatgaaag
     1981 tagtagtaat tggctgtact catgcaggta ctgcagctgt aaaaagtatt ttaaaaaatc
     2041 atccagaagc agaagtgaca gtatatgaaa gaaatgacaa tatttctttc ttatcttgcg
     2101 gtatcgcact ttatgtcggc ggagtagtaa aggacccagc aggcttgttc tactcaaatc
     2161 ctgaagaatt atcttcatta ggagcaacag ttaaaatgga acacgatgta acagacgtcg
     2221 atacagaaaa taaaacagta acggcaaaag atctgaaaac aggagaaaca gaaacggttt
     2281 cttatgataa attagtgatg acaactggtt catggcctat tatcccaccg attagtggaa
     2341 tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca agcaaatgaa attattgcac
     2401 aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg atatatcggt attgaattag
     2461 tagaagcttt tgtcgaatct ggaaaacaag tgacattgat tgatggatta gatcgcatct
     2521 tgaataaata tctagataaa ccattcacag atatcttaga aaaagaattg acagaccgcg
     2581 gtgttactct tgcgctaggc gaaaatgtta ctgaatttat cacagatgaa gaaggaaaag
     2641 tgaaacaagt agctacacca acagatacat ttgatacaga tatggtcatt ttatgtgtgg
     2701 gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga tacactgcca aacggggcaa
     2761 tcaaagtgaa tgaatacatg cagacaagta atccagacat ttttgcagct ggagactctg
     2821 cagtagtcaa ctacaatcca agtggcacgc aaaactatat tcctttggca acaaatgccg
     2881 ttcgtcaagg tttgcttgtg ggaaataact tgacagatca taaaatggcg tatcgtggta
     2941 ctcaaggaac ttcaggactt tacctatttg gctggacgat cggttcgaca ggtgtgacca
     3001 aagaaagcgc aactttgaac gatctagaag tccaagcgac tgtatttgaa gataattatc
     3061 gtccagagtt catgccaact actgaaaaag taatgatgga gctagtctat gaaaaaggaa
     3121 cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga cattacacaa tcagcaaata
     3181 ccatgtcttt agctgttcaa aataaaatga ctgtagaaga tctcgcactt tcagatttct
     3241 tcttccaacc acattttgac cgcccatgga attacttgaa cctacttgca caagcagcgc
     3301 taggcgaaat cgaaactgca caataaagaa aaagggttgt gacacaattt tgtcacgacc
     3361 ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc ttcgaatttt ttgtcacaat
     3421 aagcagcaaa gataaagctg ttacataaaa gaacaagcag ctttatttga ttttgaaaag
     3481 aaaggtgaga aagaagatga ataaagaata ttttgtccaa caagatgcat ttttagaaga
     3541 ttataaaagt gcttcgaaga aattaacgat caatattgat gagttggcag atcaagtact
     3601 ctctcagttg aatgaaacgc aagaggaata ttacacgatg ccagcaaatg acgccaaaga
     3661 taatgtttct catcgttttc ctttcagtag acgcatctac tcagatgaaa atgacggcac
     3721 attgaataca gagtacacat atgaaggtca gccatatgtg ctagaaactg atgatgatct
     3781 ttaaagacag tttttgaaaa ggccgctcat ccttaagtga tgacaaaaaa tcatcacaat
     3841 taccgatgac tgtctcttta ctatctttta atcagaaaaa ggggttatga caagctttgt
     3901 caaccccttt tt
//
LOCUS       DACDLI010000132         3870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDLI010000132 DACDLI010000000
VERSION     DACDLI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.134"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..176
                     /locus_tag="IV353_002668"
     CDS             <1..176
                     /locus_tag="IV353_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9010833.1"
                     /translation="FMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQT
                     VVFKGKRNLENKK"
     gene            complement(367..1899)
                     /locus_tag="IV353_002669"
     CDS             complement(367..1899)
                     /locus_tag="IV353_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9010834.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            2133..2834
                     /gene="nagB"
                     /locus_tag="IV353_002670"
     CDS             2133..2834
                     /gene="nagB"
                     /locus_tag="IV353_002670"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9010835.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3088..3801
                     /locus_tag="IV353_002671"
     CDS             3088..3801
                     /locus_tag="IV353_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9010836.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
BASE COUNT         1169 a          671 c          804 g         1226 t
ORIGIN      
        1 tatttatgtt tatgtacttt attggaggtg cggctggttc attgctaggg acgacaatgt
       61 ggcagcagta tggctggtta ggtgtaacgg ttagcgggct tgtttttcaa ggatgtgcgt
      121 tcttctttca aacagttgtt tttaaaggaa agagaaatct cgaaaataaa aaatagacga
      181 aaagaggttg tgaaaagctg ttctgcatca agtaataaga atagccaaat aaaaatagct
      241 cctcatattt tgccgttttt tgagctaata ccgaaggtca gctaattggc caccatttat
      301 tcagaagaag gagttgtgac aaaacttttg tcacagctcc tttttttcaa ctattcaatc
      361 aaacgtttat tttggttcaa tctcaatgac tcgttgcaca agatcttgat aaaattcttg
      421 ttcatgagag acgatgatta ctgttcctgg aaatttctgt atcccgtctt gtaagctttc
      481 tttcgtttct tgatcgatat ggttcgtcgg ttcatcgaga atcagtaaat taccttggtt
      541 cattgtcaac tgtgcaagtt tcaccttagt ttgttcccca ccacttaatg tagtgagagg
      601 ttcacttgct aattgattaa ctaaccctgc tttagctagt tggcgacgta attctttgat
      661 agtcgctttt ggaaattgac tgcttaaata ttgtagtggt gtttctaatg gattttccca
      721 gtacagatct tgtgaaaagt aattgatttt tgtgttatct gggaagtgga aatcgccact
      781 tattttttca atctcaccga tcagtgtttt taataaagta gattttccaa tcccgttgaa
      841 gcctcgaatg gcaatatttt caccaaagcg gatggtcaaa ttgatgggtg caagtagcgg
      901 tcgatcatat ccgatgacta aatcagttgt ttccactgct aatgtcgtga caataggcga
      961 gaatggaaaa tcaaagacag ctttacttgc tgaagctgga ggcgtcagcc gttcgatttt
     1021 atctaattgt ttttgtcggc tttttgccat cgttgatcgg gaaccggctt tgtattttcg
     1081 aatataagct tcggtctttt cgatttgttt ttgttgagca tgatattggc gcaaatagct
     1141 ttcgcgattt tgttcctttt gtttcaatgc ttgttttaga tggccagtat acttagtaat
     1201 ctttccaaac tcgatgtctg cgatatgggt ggtgatctga tcaagaaatt cttggtcatg
     1261 ggaaatcacg atatacgcac cgtcaaaatc ctgaagaaac tgcgtcagcc attggatatg
     1321 ctgatcatct aagtgattgg ttggttcatc taagatcaga acatctgggt tttctaaaag
     1381 tagttttgct aaaatcacct ttgaacgttg tccaccactt agatttttca acagtgaatc
     1441 taaacctaag acatcgatcc ccaacccgct gctcatctca gcaactaagg tgtcgatttg
     1501 ataaaacact ccttggtcta attccgtttg gagtttccca gctttttcca gcaaggaatc
     1561 atcgaaggtc tcgctatatg tttggtaaag aaaagcaatt ttttcttcct tttcgtattc
     1621 ttttgcgtac gcagttttta gaaattcttc gattgttaat gtttcatcga cttccacata
     1681 ttgatcgagg taacctttgt gaatattttt ttgccaatgg atctgtccgt catctggtaa
     1741 gatctcacca gtgatgattt taatcaatgt acttttaccg gctccgtttt gtccaatcag
     1801 cccaagatgt tcgccagcat ttaattgcag atcgacacct tcatagagga ttttgtcgcc
     1861 aaattgctgg gtaatgttgt ttaatgtcag tatactcata ataactcctt tttcataaaa
     1921 aaactgcaga cataaagcgt ccacagtgaa tcacttgctt ggttttcatg caacatggat
     1981 tgtttgattg aaccatctta tgaaacctaa cacggattcc tatttattgt caagaaaatg
     2041 aaaaaaatgc aatttggtct atactaatta ttgtaaaaag aaaaaaagaa tgctataatt
     2101 gggctatacc aaaatataaa ggagcaatca ctatggaaat cattcgtgta aaaaacgctg
     2161 aagaaggcgg aaaaaaagca tttgaaatca tcaaagaagg tatgaacaaa ggtgcaaaag
     2221 tattaggatt ggcaacagga agcacaccag ttactttata taaagaaatg acttcaagcg
     2281 atttggattt cagtgaaatg acttctgtaa acttagatga gtacgttgga ctaggcggtt
     2341 cagatgaaca aagctaccgt catttcatgg atgtccaatt atttgataaa aaaccattca
     2401 aagaaacgtt tgtaccagat ggaaaagcag aagatctgga agcagcatgc aaaaagtatg
     2461 atcaaatcat cgatgcacat ccaatcgata tccaaatctt aggaatcggg caaaatggac
     2521 atatcggttt caatgaacct ggtgcttcat ttgacggaac gacttcagta gttgacttaa
     2581 cagaatcaac aatcaatgca aacaaacgat tcttcgataa agcagaagat gtaccaacaa
     2641 aagctgtttc aatgggaatc ggttcaatta tgaaaagtaa agaaatcgtc ttgatggcat
     2701 ttggcgaaag caaagctgat gcaatcaaag ggatgattga aggacctgta acgaatcatt
     2761 tgccagcaag tgtattgcaa aatcacgaca atgttgttgt gatcatcgat gaagaagcag
     2821 caagtaagtt gtaattttca atagtaggct aaagaggttg tgaaatcagc gttttgacct
     2881 gagtattaga gaagacaaag gaaaaatagc tccccatatt ttgccgtttg ttgagtaata
     2941 ccgagggtca gctgattgaa caccgtttat tcataaaaag gactatgaca aaaattttgt
     3001 cacagtcctt ttttcgtaag aggtaaaaat tgcacttttt ttgtctgctc ctttcagaca
     3061 tgatatattg gactggaggg tgtttagatg aatgatatgg tattgacaat tccagagatt
     3121 attttacgtt tagggatcgc tatgctattt ggcggagcaa tcggttttga acgacaatat
     3181 aaaaatcgtc cagcaggtat gcgtacgcat attttagttt gtatgggagc atgtatcatt
     3241 gcattgatcc aagttgagat cgctagtggt gcattaagag atgctttaga gtatccacag
     3301 cttgctggaa cgattcgttc agatgaagca aggctgattg ctcaagtggt gagtggtgtc
     3361 ggtttcttag gcgcgggaac gatcatcgtc acgaaacaat cggtgacggg acttacgact
     3421 gccgcttctt tatgggcaat tgctggatta gggatcgcca tcggcatggg ttactattcc
     3481 attgcgatca tcagttttat cgggattgct attgcactca cagtagtgaa acgcgtgatc
     3541 catgtaccta cgactaaaaa gttagaaatc caatttatcc atagaaaaga aaccaaagat
     3601 tttcttaccg attattttga agagaaaaat atcgtaatcg aagatgtgaa ttttgacgta
     3661 aaattcgttg atgactaccg agtgtacaaa aatatctata cgattgatct tccaaaaggg
     3721 ctcacttatg ccgatgtgat cgaggagttg tccgtacaca aaaatatcac aaagttgcat
     3781 ttagtcagta ttgcgcaata aatgatctga ggttgtaaaa tagatgtcct acatcaagta
     3841 ataagcccag ccaaataaaa atagttcctt
//
LOCUS       DACDLI010000133         3785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDLI010000133 DACDLI010000000
VERSION     DACDLI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.135"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..171
                     /locus_tag="IV353_002672"
     CDS             <1..171
                     /locus_tag="IV353_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9010837.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            346..642
                     /locus_tag="IV353_002673"
     CDS             346..642
                     /locus_tag="IV353_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010838.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="IV353_002674"
     CDS             649..2601
                     /locus_tag="IV353_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9010839.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IV353_002675"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IV353_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9010840.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="IV353_002676"
     CDS             3400..>3785
                     /locus_tag="IV353_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9010841.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DACDLI010000134         3755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDLI010000134 DACDLI010000000
VERSION     DACDLI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.136"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            91..1704
                     /locus_tag="IV353_002677"
     CDS             91..1704
                     /locus_tag="IV353_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9010842.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            1876..3177
                     /locus_tag="IV353_002678"
     CDS             1876..3177
                     /locus_tag="IV353_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9010843.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         1196 a          703 c          716 g         1140 t
ORIGIN      
        1 tattcaaaat aaacaataat tttccggcac aattatttgc tgttttttac cataacatag
       61 tacgatcaac gtagaaaagg agatgttttt atgaaagttg ttgtcattgg cggtgtagct
      121 ggcggtcctt catttgccac tcgtttccgt cgattgaatg aagcgcacga aatcattatc
      181 tatgaacgcg gagaaaatat ttcttatgca agttgtgctt tgccttatta tttaggaggt
      241 gtgatcactg accgtgactc gctgatcgaa cgtacaccag aaatattgaa aacaaaaaac
      301 aacatcgatg tctttactaa acacgaagta acagcaatcg atccttctac taagcgatta
      361 acagttaaag acctatccac aaatgaagaa ataaaaacag attacgataa gttgatcatc
      421 tcttctggtg ctagaccaga ttatccggat attcccggag tttttgaagc agaaaacggc
      481 tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat cgttccttga agaaaaaaat
      541 ccacaacatg tcgtcattct tggtgcaggt gttatgggtc tggaattagc tgagaatttc
      601 aagcatcgcg gcttagacgt gactttaatc gatcaattgc cacaagtcgc tttcccttat
      661 gatccagaaa ttgctaattt agtttatgac aaattgctga aagaaggatt agccgttcat
      721 ttagaaacaa gagttactga gatccgtgat aaaggtcgag aaatagtatt atcagatggc
      781 tccgtccttt ctgctgatat gctaattttt gctgttggtg tttctccgaa gaatgaagtg
      841 gtgaaagcaa ccggcataca attatcagat acaggtcaga tcattgttga tgaccagtta
      901 caaaccaatc tgccggacat ctatgcgatt ggcgatatta tcgaaacgac tagtgtagtg
      961 actggtcagc cgatccaaag tatgctttcc agtgcggcca atcgtcaagg acatatgctg
     1021 gcagatattt taaatggtac gcctatgcgc tatcgcggat atattggtgc aggtgtcgca
     1081 aaaatctttg atcatacagc aagttatgcg ggaatgacag aacatgaatt aaaagcatca
     1141 ggcataacaa attataaaac tgtttttatc actccctttg accatgccta tttctatcca
     1201 gaagctacaa gattaaatct aaagctgatt tttgatgcag atagcggtcg tattttaggt
     1261 ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg gagaactttc tgtagcaatc
     1321 accggaaact tgacagtctt tgatttgcct gatttggagt tgccttactc tccaccgtat
     1381 tctactaccc gtgacccgtt gaatatagct ggttatgtcg caatcaatca aatgacgaat
     1441 atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg atttgaaaga agcgttcttt
     1501 ttagatatac gtgaacctaa taaagcacct tctggcagta tctcggcaac aaaaaatatt
     1561 ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa aagataaaaa agtttacatt
     1621 actttcagaa gaggattgaa tacttatact tctgcccgaa tcttggcagg tttgggtatc
     1681 aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg ggtagccgcg gtctaggagt
     1741 tatttttact ggctctataa tttctaggac tgatagaaaa tcattctatc ggtccctttt
     1801 gagcatgaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     1861 aaaggagcgt tctgtatgcc tgatcttcat catatcaaac tactacttgg aatcaaagat
     1921 aaaaacattt tcatcacaaa tgtggaatcg aagagcatta agaatatcaa gagtcttgtt
     1981 gtttctgcga cgttaagtaa agaaattcgt cgctgtcctt tgtgtaaaca gatgaatcat
     2041 gaaggcatga tcgttaagaa cggaaagaaa aaatcgctca ttcaacttaa taaatgtgcg
     2101 aaccaactga cttatcttgc tttagcgaaa caacgctacc actgtcgtgg ctgccacacc
     2161 tattttacgg cgaataccta cattgtcgat agaaactgct ttatcgctaa acaagtgcgc
     2221 tataaaattt tagaagagct aactgaaaaa caagcaatga cgacaattgc taagcattgc
     2281 ggcgtttctt ggtcgaccgt ttctcggaca ctcgcttctc tgatccccat gacaaaagta
     2341 aaaagaaatt ggttaccgcg ttgtttatta atggatgaat ttcgttcact gaaaaatcaa
     2401 gtcggtccct actccttttc ttgtatggat ggcgatactg gaaaattact ggatattctt
     2461 ccttcacgga agaaaaaggc tctcgtttct tattttatgc aatttgaacg acgcgcacga
     2521 ttgaaggtaa aggtcatcgt gacggacatg aatgtttctt atgcctctct gataaaggaa
     2581 tgttttccaa aggcaaaact cgttgtggat cgttttcaca ttgtcaaaca tctcattcgt
     2641 aaatttgagg atattcgcgt acgaatcatg aaaagctttg atcgaaacga tccaacacaa
     2701 gccaaatatt atcgacaatt aaaggcgttg tctcggcttc tgatcaaacg acaggacaag
     2761 cttgtttatg acaaatggac caaatggcgc aattttggtt gggcgtatct caccgaaagt
     2821 gaagtggtgg atcgtctgct ttcgatctct gacgaattac gaatcgccta tttttactat
     2881 caagaaattc ttcaagcgtt tcacgacaaa gaggcggaca ctttctttaa acttgtaaga
     2941 acgatgccca attccgttcc aaaagaactg cattctataa aaaaagcttt tataaactat
     3001 gaatccggca ttcgtttagc cctcgaattg ccttattcaa atgccaaaat agaaaatctg
     3061 catacccaca tcaaagcatt gaaacgagtc gcttatggct ttcgaagctt tcggaaaatg
     3121 aagaccagaa ttttcttact aaataatctg attacctatg aatcaaaaaa catctaaagt
     3181 agaaaaggaa ttctacctta gatgttctta tctcactcta tcagtcctat ttgacaaaga
     3241 gcctttttac ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata
     3301 tagaatagaa gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata
     3361 gtttaagaaa gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa
     3421 aacatgttgt gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc
     3481 ctaatattac gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt
     3541 cgaatatttt ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac
     3601 tggcttgcgt tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc
     3661 atatttttcg ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta
     3721 ttaaataatt taattttttt gttcctattt attta
//
LOCUS       DACDLI010000135         3665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDLI010000135 DACDLI010000000
VERSION     DACDLI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.137"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(182..517)
                     /locus_tag="IV353_002679"
     CDS             complement(182..517)
                     /locus_tag="IV353_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9010844.1"
                     /translation="MGIEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            803..2683
                     /locus_tag="IV353_002680"
     CDS             803..2683
                     /locus_tag="IV353_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9010845.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2762..2989
                     /locus_tag="IV353_002681"
     CDS             2762..2989
                     /locus_tag="IV353_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9010846.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2994..3536
                     /locus_tag="IV353_002682"
     CDS             2994..3536
                     /locus_tag="IV353_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9010847.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
BASE COUNT         1205 a          607 c          754 g         1099 t
ORIGIN      
        1 tgaagataat atgattctat cattgtcagt tcgtctatgg ttacactcct ttgttttctt
       61 tcgtcggaaa tacaatttga gtgtaccata aatgcctttt tatttttcta acttaatttt
      121 acaattcgcg tatcactctt aaacaataag ttaaaaaaat actcgtttac ataaaaaaag
      181 attatttaga tgcgataata tcatagataa agaaacattc atcataagaa acagtgatcc
      241 caaattctgt ttcaatattt ttgaaggcct tttttacatg aaaaattgtt tcatgagaag
      301 gtcttgtgtc atcttctgat ataattttaa gttcatgacc gtttatcaat gtacgctcaa
      361 ttagacacgc tgtatgaatc aaaagtttta aaagaatgga atgagtgaat tttttgtgga
      421 aagtacattc caattcttgg ataaaatcca ttaagctttt ttggatctta ttcccagcta
      481 ataagtagag atatttgtcc actgcttcaa ttcccatatc aaaaaacgat atttcttgtg
      541 aatgagtggc tgctgccctt ttttgattgt tgttcttcag tcgtaagatg acaaagcatc
      601 ttatctttga ataaaaaaat agaactacct aaggaagcgt ttcggcgctt cctttttcta
      661 tatgatcgtt taatgactgt aggaattcag tataccaaaa gaaaaccgcc tttttgaata
      721 aacttgatag tggtcaattt tttatgatgg ataagtatct atacttagtc tcgtaagaat
      781 gaaaaggaaa agagggagaa agatgaaatt acaaaagtgg atccaagagc atcgaaatca
      841 tatcaccgcc ttatcagctg tgttgattat ttttggtttt atcgggaaat ttactggatt
      901 tgtcttgttc tataacattg ctctagccat agcttctatt attgcggtca tacctattat
      961 aatccatgcg tatcaggcag taaaagtcaa agttatctca attgatttac tcgttactat
     1021 tgctgtgctt ggtgccttta tgatcggaga atacaaggag tcagctatcg ttacattctt
     1081 gtttttgttt ggtagttatc tggaacaaaa aacattgacc aaaacgcggg aatcaatcaa
     1141 ggctttgacc gagatggccc catcgactgc cagcgttgtc caagaagatg gaacgaccga
     1201 agaagtggaa atcgatgacg tggaagaagg cgatatcgtc ttagtaaaaa ctggtggagc
     1261 tattcctgtg gacggacgag tcattgacgg gaaagggtat gcggatgaag ccgcagtgac
     1321 gggtgaatca cgacaagtcg tcaaaatcag tggagataac gtattttctg gaacgatttt
     1381 agcagatggc tacgtgacga tcgaagcaac taaggtcgga gatgatacga cctttgccaa
     1441 aattatagaa ttagtcgaag aagcacaaga taccaaatca catgcggaaa aattcattga
     1501 tcaattttct cagtattata ctccaaccgt tttacttatt gcgttgcttg ttggtctgat
     1561 cagtcgtgat tttcgtttag ccatcactgt tttggttctt ggttgtcctg gcgctttagt
     1621 cattggtgcg cctgtttcga atgtagcagg aatcggtaat ggagcgaaac ggggcatctt
     1681 agtcaaaggc ggtgaagcaa tggatacttt ttccaaaatc gatacttttg tctttgataa
     1741 aaccggtaca ttgaccaaag gaactactgc tgtatccact atcaagaact atacagagga
     1801 tgaaggggac agcttagctt tagtggctcg attagaaacg ttatccgatc atcctctggg
     1861 gcgcgccgtg atccattatg ctcaggagaa agacattgat tttcaacaat tagcagtcaa
     1921 agataatcag acgatcaagg gacaaggaat cacagctgag attgatggtc atacagtttg
     1981 tgcgggcaac accaaattga ttgccgctca caaactttct ctcacaccaa agcaagtaca
     2041 agatttacat caattacaac aaagaggcag ctcagtcatt attgtagcaa tcgacaaaaa
     2101 aatcactcaa atcattggtg tgtcagatgt gattcgacca gaagtcgctg aacaattagc
     2161 tatgttgaaa caagcaggag caaaacattt agtgatgctg acaggtgata atcagatgac
     2221 tgctgattat gtagctgata tgttaggcat tgatgaagtc catgctgaat tattgccaga
     2281 tgaaaaggtt caatttgtga aaaaatacca agaacaagga ttgcacgttg ctttcattgg
     2341 agatggaatc aatgattctc cctctttggc tgcggctgat atcgggattg ctatgggatc
     2401 aggaacggat gtcgctatcg aaacgagtga cgttgtcttg atgcaatcta gttttggatc
     2461 gttggttcat gcttaccgtt tggctaagaa aactgttttg aatacccgtg aaaatgtagt
     2521 gattgccatt ggtgtcgtct tatttttact catcggctta tttgccgggt ttatttacat
     2581 ggctagtggg atgttcgttc acgaagctag tatcttgatc gttatcttta acgcgatgcg
     2641 tttattgtat tttggacacg aaagcaaaga agaaaaatcg taaaacttga taagaatcaa
     2701 tttttttatg gaagctgtaa tctagaataa gactatcaag aaaaaggaag gaagaactac
     2761 gatgaaaaaa atgattatgc aattagatac actagcttgt ccaagctgta tgcaaaaaat
     2821 agaacatgcg gtttctcaac agcctggggt aagcaatacg aaagttttgt ttaatgcgag
     2881 caaaatcaaa gcaaactttg atccagatga agtccaccct gaacaattag ctgcagttgt
     2941 ggaaaaatta ggttatgaag tccaatcaac aaaagtaaaa gaggcgtaaa tcagtgaata
     3001 tagtggaaga aaaatttgct caagaaacaa aacaagcaga tatcgatcat cataaaccaa
     3061 ctgctggcgc catggtgggg catgtattgg caaattttaa attagaagaa atgaaattga
     3121 tccaagcaca attgtatgtt ttgggtacat cttcgcaaga attaagtacc cttttttcag
     3181 aatatgctga tcaagaagcg agatggttca ataaaatctc tcatgagtta gtgattgaaa
     3241 atgaaatcgt tccgtctact ttagaagaag tcatgcgtta tggaaaaatt tacgaacatg
     3301 gtcaaaacaa atatttacca gcctcaacaa tgttggaaaa ctttgcaaaa gattttgatt
     3361 tccaaaatgt ctttatcaca cgggcaatca aattagctga aaaagaagaa aaatatggct
     3421 tgaaacaatt gcttgttgaa cttttaagtt tcaataaaga aatcatctac caagtacaaa
     3481 gactccaagg aaaaactgtc cgacaagatt tggatgagga agacgatgat gactaaaagt
     3541 ctatgtttct tcaaatacaa atataaagcg tgaggtaaat tggaaaatca cgaagattaa
     3601 aataaaagaa gcacccgctg atgtattaca gacaacatca gcgggtgctt cttagttttt
     3661 aaaag
//
LOCUS       DACDLI010000136         3640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDLI010000136 DACDLI010000000
VERSION     DACDLI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.138"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..844
                     /locus_tag="IV353_002683"
     CDS             155..844
                     /locus_tag="IV353_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9010848.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            858..2360
                     /locus_tag="IV353_002684"
     CDS             858..2360
                     /locus_tag="IV353_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9010849.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2373..2849
                     /locus_tag="IV353_002685"
     CDS             2373..2849
                     /locus_tag="IV353_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9010850.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(3008..>3640)
                     /locus_tag="IV353_002686"
     CDS             complement(3008..>3640)
                     /locus_tag="IV353_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9010851.1"
                     /translation="RLFSKGIFDVDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNY
                     PDFNSEFGHLEDDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSR
                     FPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLP
                     KSMDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         1163 a          520 c          703 g         1254 t
ORIGIN      
        1 tcctctacaa tttcaacttt tctccattat actccaaaat aataaaaaaa actcctaaat
       61 actccatttt tatctttact tttatgaaag cggatgctat attgtttttg gagttgttat
      121 ccaaaaataa taaaaaattg gagtgtttag gataatggat aaaaagaggt ttttagatct
      181 tatttcaaaa ggtttgattg tttcctgtca agcattacct ggtgaaccat tttacaaaga
      241 ggaagaaagt ttaatgcctt attttgcttt ggctgctcaa agggctggag cagttggtat
      301 acgtgcaaat tctgttcgag atattattga aattagaaag tgtgtatcat taccaatgat
      361 tggaattatt aaaagagatt atccaccaga atctccattc attactgcca ctatgagaga
      421 agtagatgaa ttagtagaaa ccggtgttga ggtaatcgct cttgattgca ctcttagaaa
      481 tcgacatgat ggactatcta taaattcttt tattactaaa ataaaagaaa aatatcctca
      541 tcagttattt atggcagata tttctacatt tgaagaaggc aaaaatgcat ataaagcagg
      601 aatagatttt gtaggaacta ctttaagtgg atatactgag gaaagtaaaa aacaagatgg
      661 gccagatata gatttgatta aaaaattggt agaagaaggg atttgtgtaa ttgctgaggg
      721 aaggattcat actccagaac aagcaaaaga gataaaaaaa ataggagttg caggtattgt
      781 tgtgggtggt gctattactc gaccacagga aattgcatca agatttgtta aagcattaag
      841 ttagggggag aaataaaatg tttaaaaaat tttcacaatt aggacgcgct ttcatgttac
      901 caattgctat attaccagta gccggattac tattgggact gggaggagcg ttaacaaatg
      961 agagtgctat aaatgcttat ccatttttag atcaaccgtg gttgcataca attttaagta
     1021 ttatgagtta tgctggaaat gcagtttttg caaacctagc actaattttt gcaattggta
     1081 ttgctgtagg acttgcaaat ggagataaag gaacagctgg tttagctggt ggagtagctt
     1141 atctagtata tactgcaact attagtgggt ttcttgaact tttttcagca aaagatgcaa
     1201 cactagatac aggtgtagtg ggatcaattg ttgttggtgg tactgttgca tttttacaca
     1261 atcgttatag aaaaattgaa ttaccacaat tcttaggttt ttttggagga tcaagattta
     1321 ttccgattgt ttctagttta gctgcaatca ttattggttc tttcttttat ttaatttggc
     1381 caccaattca aaatggttta gtagttgctg gtgaacaaat tgcccaaatg ggtagtttag
     1441 gaactttttt atatggtttt ttattgcgtt taacaggtgc tgttggacta caccacacca
     1501 tttacccttt gttctggtat acatctttag gtggtaccga aactgtagct ggatcaacaa
     1561 ttgctggagc ccaaaatatt ttctttgccc aactagctga tccaaaccac actggtctgt
     1621 ttacttatgg tactagattt tttgcaggaa gatttgcaac aatgatgttt ggattaccag
     1681 cagcatgtta tgcaatgtat cgtgcaattc ctaagaaaaa tcgtaaaaag aatggtgggc
     1741 tttattttag cggggccctt acatcatttt taactggaat tactgaacca gttgaatata
     1801 tgtttttatt tgttgctcct tggctttatg tgattcatgc attcttagat ggattatctt
     1861 tttattttgc tgatatctta aatattcgtg tagggaattc cttttcaggt ggtttgatcg
     1921 actttttatt attcggtgta cttcaaggga atgacaaaac taattggtta aaattaattc
     1981 cttttggtat tgcatgggca tttatatatt tctttgtttt tacattcttt atcaaaaaat
     2041 ttaaggtagc tattcctgga atggaagaag aatcagaagt tcattctgaa aaagtaataa
     2101 acaaaaatac atcattacat gaagagtctc ttatcattat tgaagctctt ggtggaaaag
     2161 aaaatattga gtctgttgaa gcttgtgcaa ctcgtttgcg tgttgctgtt acgaatgggg
     2221 agattattca aaaacaaaca ataaaagaca ctggtgcaac tgctgtcttc gaagtgaagg
     2281 gtggcatcca agctgttttt ggagggaaag ctgacttatt aagtcaagaa atcaatcaaa
     2341 tattaggaaa agataattag gaggttttta atatgtttga aattttcaaa aagaaaaaac
     2401 aagaaacttt ttatgcacct tgttccggaa aaatcatttc catcacagaa gtaaatgatc
     2461 ctgtcttttc acaaaaatta ctaggtgatg gttttgccgt catacctgat ggttcaaaaa
     2521 tttatgctcc attggctggt cgagtaacaa atatatttcc tactaaacat gctattaata
     2581 taaaaagcaa ctcaggcatt gattatttga ttcatattgg aattgataca gttgaattac
     2641 aaggagagcc tttttcgatt tgtgttcaag aaaatgatga ggttactcct gaaacctcgt
     2701 tggttgatgt ggattttgac aaaataataa aatctggtaa agatacttct gttatagttg
     2761 tttttccgga gaaaaatcag ttcaatgatt taataataga taatggtcga attgctcatg
     2821 ggaattcatg tggaaaaatc actttttaaa aaagtgtata taaaaagcta taaaataagc
     2881 taaataggat atttgtcaaa tgaagttggt gaacagaaag aactgttttc tttctgttca
     2941 tttttttatt ttattagtaa tgattcccca tgccaaatca tgatccgtaa tttaaagtta
     3001 tgatttgtca aattaagcta gaataaaatg cttcttgtac atagctcaaa aatatttcga
     3061 ttggtgttct gtaattcaat gattttcttg gaatatgatt acgttgattg ctgacactgg
     3121 aaataaatgt ctgattcact tctctaaaat ccattgattt cggcagtcca ttacgacgca
     3181 gaatcccgtt agagttctcg tttaatgggc gttgagaagg tgttccaggg tccgcaaaat
     3241 atatatcaat atcatgttgg ttactaatgg ctttccagtt agaaaattct tttccacagt
     3301 caaacgtaat agatttaaag aagtttttag ggaagcgaga aaaccattga ttaagggcag
     3361 tttcaatatc taatgcctta cggccgttgg gtttaatcgt gataatgact ttagataatc
     3421 tttcaactaa agtaatgacg gcacttttat gatgaatgcc aacgatcgta tcatcttcaa
     3481 ggtgaccaaa ctcagaattg aaatcaggat aattatcagg tcgatcatgg attgagcgct
     3541 gatactgttg ttttccccgt ttttcctgat ggccattggg ttttctttta cctttcatcg
     3601 gtagtgtgtc aacatcaaat attcctttag aaaataaacg
//
LOCUS       DACDLI010000137         3555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDLI010000137 DACDLI010000000
VERSION     DACDLI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.141"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            304..1344
                     /locus_tag="IV353_002687"
     CDS             304..1344
                     /locus_tag="IV353_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9010852.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPITAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2168..2500
                     /locus_tag="IV353_002688"
     CDS             2168..2500
                     /locus_tag="IV353_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9010853.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3119..3460
                     /locus_tag="IV353_002689"
     CDS             3119..3460
                     /locus_tag="IV353_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9010854.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1315 a          471 c          657 g         1112 t
ORIGIN      
        1 atgcataagg tagttcttaa tttatttttt gttttgacac tggtagtact taattatctt
       61 taattttaaa actaaatttt attcatacgt ttttctggta gtatttaatt ataaataata
      121 tttatttaaa gtagttctta atgactttaa aggtagtaca taattcattt tatttctgat
      181 tttaggtagt atttaattaa aaaacagttt taatgcgttt gcatttttta tttatctttg
      241 ctattttcga aaagagtagg taattttaca taaataaaac aacgtgaaag tagaggatct
      301 acaatgactg attttaaatt ttttaaggcg gatagagttt acaacgaatt attttatcaa
      361 tttccaaaag tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt
      421 gcctatatgc ttttaaaagc aagattagag atcgcaatca gcaaacggca aatcgatgaa
      481 gaaggtaatg tttattttac ttatacgaca aatgaactat gtagagtatt aaactgccaa
      541 aaacaaaaag cgatagcaat caaaaaagag ttggaatcct ttggtttatt attacaaaag
      601 cagatgggat ttaacaaaca gttagggaaa aataatccta atagactata tctagcagaa
      661 ttaaaagtct cagaaaatga tatctactta ctcgaaaaat ttgatagaga gaatagggaa
      721 aacgttgata aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa atcagacgct
      781 gaatcccttg gggctcaaga gggtatgaaa atcataccgt gccaaaacgt tgataaatca
      841 gagggtatga aaatcatacc agaacttaat aataatatat tagacactaa tagacacaat
      901 atagacactg aaaaagaccg cctacaagat caattgttgt tagacaattt tgagacaatt
      961 atgacaaacg acagcattgc tacgtttgtc cctgaacgag tattaaattt gataaaaaca
     1021 ttttcttcaa gttacagtga agctcaaaaa accgtccaga ctattcataa tgcaaagaaa
     1081 aaagctgaaa tagaaagtgg tatttcgata gtttttgaag aactcgatag ttattatgtc
     1141 aatgcagaac aagaattata cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa
     1201 aaagtcgaaa atatccagaa cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa
     1261 ccaataactg ctaaagtatc aagtgaaaaa cgtttgaatt atgaaagctc cccaagcact
     1321 attacgaaag actggttaga gtgaaacttt actgacaaag tgattgttgc ttcatggagt
     1381 aacattaaaa ttaaaaattt tttattattc taattatatt acgaatatta taaaataata
     1441 gctattttaa tagaatttat aagttaaaat attatgataa aaatcacaaa cgtagccttt
     1501 tacgtcataa gtgttaaaag aaataattaa tttcaaatat agaaaaggga acgtatgttt
     1561 gcatactcct tttttttgag ttataataat acgcgaagag agataatttc attatttaaa
     1621 caagctaaaa atcttgatat tacttctttt caggcaatac gaatattatt cgcttacatt
     1681 ttttgtataa atttaattgt ctgctatgct tgtgatgaat tgattttcgt tctgcacttt
     1741 tggatgataa atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa
     1801 ggctggtaac cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa
     1861 cctttcagtg ttacggtatt acccgcaatg gatttagtat aacagatatt caaaagtgat
     1921 accacttttt ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct
     1981 aagcataatg ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta
     2041 gagggaacgc atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt
     2101 ctctattttt gaaaaataac aagaaaaact tgttaaaaat atttaaagaa attgggtggt
     2161 ttattttatg tcatatacag gaattttgag tctcaaagat atttgtcact acggtaaacg
     2221 gtgtaccgct acagagaaaa tcacgaagaa actatcaact ggccaaagta aaaccgttgt
     2281 gcagtgtaag aaatatataa tccagaaaga caaagtctca gaggaaatga tttattacat
     2341 tgggaaacaa aagcaaataa ttttaaaaga tcctatcccc ttaaaagagc tatatccaac
     2401 aatcaaacat gtttatgacc aaaacggtgt actgattggt cgaagaaaaa atggagtctt
     2461 gcgttgtacc gcaaaaggga tggggcgttt aatcagctga attgatcatt ctcataactt
     2521 gtattttatc gtttttcgct tgcttgaaaa tattgtcgag acttctaaaa gaacttagaa
     2581 tcattagaaa aataaggagt agacttgatc tatcaacaat ttccttcggg gagtgacggg
     2641 gtagattttt acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg
     2701 atagtggtga aatgaatttg tgtaaataat atacacagag taaacaagag ggaatttaga
     2761 ggttagacaa gttagaatag cgcctagatt aatcatgagc agctactaag ggatctaggc
     2821 tttgacgcaa actcatggaa taaggagtag gtcttctcgt aatggggagg acgcacaaga
     2881 aattgtgtcg tgtgctgcag tacatgtaga tacttgcgag gacgttggtc attggatcaa
     2941 tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa cgagtcgata
     3001 ttttttatgt atttttaagt aaacgaaaaa cgataaaata tgaacgagtc ctgaataaag
     3061 tgaaaaaatt gatataaaac atagagaaaa tgaaatttac gtaatggagg aatgacaaat
     3121 gaaaaattat gatccgaata tacgattggg cacacatact attaaggttt ctttccaacg
     3181 ttgggattac aaaggttttg tgacgtttag aagaggcgga aattgtaaag gattagatgt
     3241 tttagcatta aacgaggatg atttatacga tcaaacattg actgataatc caattggatt
     3301 cggcttgcta cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga
     3361 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
     3421 ctttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
     3481 acagataaag gcttagaagg agtcacgacg attaataaaa aggtagtatt accttaggca
     3541 ttgagtaagg aattt
//
LOCUS       DACDLI010000138         3395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDLI010000138 DACDLI010000000
VERSION     DACDLI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.142"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..793
                     /locus_tag="IV353_002690"
     CDS             <1..793
                     /locus_tag="IV353_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9010855.1"
                     /translation="NYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNTKVGDATIIR
                     FCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKL
                     NEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVN
                     DLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNG
                     GSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1023..1253
                     /locus_tag="IV353_002691"
     CDS             1023..1253
                     /locus_tag="IV353_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9010856.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1476..1574)
                     /locus_tag="IV353_002692"
                     /pseudo
     CDS             complement(1476..1574)
                     /locus_tag="IV353_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1839..2294)
                     /gene="tnpA"
                     /locus_tag="IV353_002693"
     CDS             complement(1839..2294)
                     /gene="tnpA"
                     /locus_tag="IV353_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9010857.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2537..3395
                     /locus_tag="IV353_002694"
                     /pseudo
     CDS             2537..3395
                     /locus_tag="IV353_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1124 a          585 c          590 g         1096 t
ORIGIN      
        1 aaattatcca tttttaacga agtctgaaaa aaaagtcgct gattttattt taaattctgg
       61 gaaaactatt atttatagta cgatgagtga tattaagaaa aacacaaaag ttggcgatgc
      121 tactattatt cgcttttgtc aaaaaattgg tttttctggt ttttctgatt taaaaattga
      181 aattgcaaag gaagacttta gtcaaaaaaa agaacatccg actagtgtaa agtactatga
      241 tgaaattgct aatagtttaa cagaagctct ctattccact attaggcttt tagacgaaga
      301 aaaattgaat gaagccatta aaatgatcac tcaatcaaaa aacatatata tatttggagt
      361 gggatctagt ggaaatacta gtcttgattt agaaaatatg tttcttcgcg taggagttca
      421 agcaaaagct gtactagacc ctcattttca agcccaagtc gcttctttat taacagttaa
      481 tgatttagta atcatttttt ctttatctgg gaaaacgaaa gatacttatg attcattgaa
      541 aatagctaaa aaaaatggcg caaaaattat tgctataact aactatattc attcacctat
      601 aggaaagtct gcagacttag ttttacaaac agccattgaa gaatttctta atggtggatc
      661 tttagctgga aaaatatcac aactttatat ttgtgatcta ttagttcatg gttatgaaca
      721 aaacaataat attgattcag ttgaattaag agaaaaagtt ttgcgctcta ttattgataa
      781 aagtattgag tgaactaccc ccacttagct aaacctaacg gtttttaacg attgaagtag
      841 gcaattcttg ggatgagagc atacttgata gcttattttt tactaatagc ggtggctctt
      901 attgaccaaa tatccctgta gttcctacgg ttcttgattt tatctaaaga tatttttatc
      961 gtggaagcta ttgatcaact tgaaagaaaa acgtgtagct cattatcact agtttatcga
     1021 gtatggatga agcaatggat catcctctct taaataaatt tattaaggat ctgatccttc
     1081 aaattttttc catgatcgat gagcaggaac ctactgaatc taaacgacat cgggcacaag
     1141 gaattattaa gattgctaag cgaatgtttt ctataaaggg tgttctaagc tatacagtgt
     1201 taattcaaaa gattcccaat catcttgttt ataagaacaa cgttgaagat taaaaaagtg
     1261 tttcaatgct aaaattgcaa aatacttcaa tgttatgaga caaactgtct ataagaatca
     1321 atttccaccc atttcatcac ttgttaaaag tttatgatcc ttttgatttt tatattaatt
     1381 tatctgtatt taatacagat tttatttatt agttcatctg cctattgtat tttattttgt
     1441 tgccttacca tctgaagtaa atatactctt aattgtcaaa ttaagttaga cagatcatca
     1501 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aatccagtga ttttctagga
     1561 agattatttc tgcgccagca ttttctaatg tttgaatttg tgttttgtaa tcttggttgc
     1621 ttgtgctaat tcttgaatag ccgtaaattg ccaaaatttc atcacccttt ctggacataa
     1681 gtttctgaca ccagttaaaa tcaagtctat cagtaaccca tacacccgtg aggacgggtg
     1741 tatttttaat ccccctagaa ggggtcgaca ctcgctgccc cttaaaaggg gatttttaaa
     1801 agaccgccaa ctgctcatct tcactcactt acttctcttt attttttaaa tggttctacg
     1861 tactctcttt tacttataat atatcgaatg cgatcatcag cttcttgttc acggatgtat
     1921 ttttcatttg tcttttgatt caatccgact gtactcacat agtatctctt cgaccaaaag
     1981 gatttgtttc cgtatttata tttgagattt gcgtgctgct cgtggattag taccgcacta
     2041 cgacctttca gataccccat aaaactggat atacatattt ttggtggtac tcgaacaagc
     2101 atgtgaatat gatctagcat tgcatgtgct tctatgattt ccacatcttt catttcgcac
     2161 aacttcctaa gtatttttcc aagatctttt cgtaattgcc catagattac ttttcgttga
     2221 tattttggtg taaaaataat atgatactta caattccaac gagtatatgc taaactttta
     2281 tcgtcatttg acatgaaaaa actcctttcg atctgatgag cggttggtag ccactcacta
     2341 gtatatcgaa aggagttttt atcgctaaag atacttcttc cacgcgtaga aacgcgcggt
     2401 tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag tcattaagaa aataaaaggg
     2461 tctaaaactt ttaagttttg taccgacccc caaaagttag accaaaaaat ctaacttttg
     2521 gaggtcggta ttttttatgg ctaagtatac ttttgaactt aaattaaaga tcgttcatga
     2581 ttacttggat ggtaaaggcg gaagtgatta tctggcaaag aaatattcca ttaaggctcc
     2641 atcacaagtg aagcgctgga tcaatgccta tcaagaattt ggtgaagagg gtctagtaag
     2701 aaagcgacaa aagaaaaaat attctgttca atttaagctg gatgcgatag agttatatct
     2761 aacaagtgag ctgtcctatc gtgaagttgc caacacactt aacatgaaca accctaattt
     2821 gatcgctagt tggatgcgtc aattccgtga gggtgggatc gatggactct caaaaacgaa
     2881 aggatgtcca cctatcttgt ctaagaaaaa tgaacccaaa aataagaaaa aatctacaac
     2941 taaagcaacg tctaaagaac gtgaacgcat cgaggagttg gagaaacgag tccgttccct
     3001 tcaaattgaa aatgcctttt taaaagaatt gaggaagctg cgaaaacagg aagcccaaca
     3061 acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc gaggaccgtt taaattagtt
     3121 gagcttctag aaactttaaa gttccctaag gcaatcttta tgtactggca aaaacgattg
     3181 gaccgtaaaa atccagatca agagattgaa gcggaaatgc ttaaagttcg ggagaagcac
     3241 aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt ctctatcaat
     3301 aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa aagtaacgac atacacacgt
     3361 aaatcacggc gctacaattc ttatcgagga caagt
//
LOCUS       DACDLI010000139         3390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDLI010000139 DACDLI010000000
VERSION     DACDLI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.143"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2303)
                     /locus_tag="IV353_002695"
     CDS             complement(<1..2303)
                     /locus_tag="IV353_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010858.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="IV353_002696"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="IV353_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          922 a          763 c          548 g         1157 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa
//
LOCUS       DACDLI010000140         3251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDLI010000140 DACDLI010000000
VERSION     DACDLI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.145"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            98..343
                     /locus_tag="IV353_002697"
     CDS             98..343
                     /locus_tag="IV353_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9010859.1"
                     /translation="MVDKQRNQHYPLVPISIILSALKEDPISIKMILKHYHGYITKLC
                     LKNGFNEVGQFITYVDEYMLRQLEIKLIEAILKFKVN"
     gene            647..1003
                     /locus_tag="IV353_002698"
     CDS             647..1003
                     /locus_tag="IV353_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9010860.1"
                     /translation="MADRLIIVNRKEETIMSKNNNKSNSSELEKYLFHPEKIEDSNNK
                     MKRINQLPLVLTNKDLKEQFQISDSTLNRLIKLGDFPSCWFGIRGHYLRDDILEWVKK
                     GNEEYFCDQFRLLRLL"
     gene            1106..2284
                     /locus_tag="IV353_002699"
     CDS             1106..2284
                     /locus_tag="IV353_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9010861.1"
                     /translation="MENMSRFKKHITATGKITWYFQAFRTTRRGFNSKREAQLAYLEM
                     EKQHRNKQKRLASNDKFSVVSQKWFEYYRSLNEQKVATYDKREEQVKILNRWIGGKKL
                     SSLSSEELQKFLFILKEKGIDGVNVGYAKNSLQSLVQVLNMVFNYCKKKNLINENPMK
                     FVKMPKYQLSVKDLKESVNSLEDKFLTVDELRAFLNYGIVYEELPMSVLFHVLFYTGC
                     RVSEALALQPQDIDFERNEILFYKQTAVKGKSKDFRIETTKTVSSARRVPVTPLVMEK
                     LQELIDVLNKTKRNSNFVVDETYLFVYLDPGKRGVPYRREYVNDHVKRCVERCGINKD
                     FHTHLARHTMASLVAEYCSWDVLKDRLGHTDSTTSKIYRHLTSTEKLKPLTAFNSLEG
                     "
     gene            2687..2980
                     /locus_tag="IV353_002700"
     CDS             2687..2980
                     /locus_tag="IV353_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9010862.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1208 a          411 c          596 g         1036 t
ORIGIN      
        1 atgatactga gataggtaaa attttcaaca tgagtacaag tggtatctgg tatcaacgaa
       61 cgaaagcggt tgaacaattg aaaatggaat atggcttatg gtagacaaac aaaggaacca
      121 acattaccca ttagttccta tatctattat cttatctgct ttgaaagaag acccaatttc
      181 aatcaaaatg atacttaaac attaccacgg ttatataact aagttatgtt taaaaaatgg
      241 atttaatgag gtggggcaat ttattaccta tgtagatgaa tatatgctaa gacagctaga
      301 aattaaattg atagaggcta tattgaaatt taaagttaac taattaaaaa gtcccctttc
      361 cactttttct atctaaaaga atcttctaat atttgagaga tatgtttttt agttagaaaa
      421 cctattgatc attgaaaatt aaataaattc tatcagtacg ttaacacttc aaggagcata
      481 ttttagttgc gccatgatct cttattgtaa agaaggagcg attaaaattc tgaaatagtt
      541 tttatagtca ataaaaagcg atgatttgaa gttgggataa tgatacttcc gtaaggctcg
      601 ccgtaaactt ggggagaggt gaaattccta tgtacttttg acaaaagtgg ctgatagatt
      661 aattattgtt aatagaaagg aagagacaat catgtcaaag aataacaata aaagtaattc
      721 atcagagtta gaaaaatatt tgtttcatcc agaaaagata gaggattcta ataataaaat
      781 gaagaggata aatcaactac cattagtttt aacgaataaa gatttaaaag aacaatttca
      841 aataagtgat agtactttaa accgtcttat aaaattaggt gattttccaa gctgctggtt
      901 tggcattaga ggacattacc ttagagatga cattttagaa tgggtaaaaa aaggaaatga
      961 agaatatttt tgcgatcagt ttcgtttatt acgattattg tagaatttat cttttcgtct
     1021 gcatgttgta taattatttc atagatagaa agattttcaa gcacttccga ttgttgggtt
     1081 ccttcgtaat tggaggtaag aataaatgga aaatatgagt cgttttaaaa aacatattac
     1141 tgcaacggga aaaattactt ggtactttca ggcttttcga actacgagac gaggttttaa
     1201 ttctaaacgt gaagctcagc ttgcttatct tgaaatggaa aaacaacaca gaaataagca
     1261 aaaaaggctt gctagtaatg ataaatttag tgttgttagt caaaagtggt ttgaatatta
     1321 tcgatcacta aacgaacaaa aggtggcaac gtatgataaa cgagaagaac aggtgaaaat
     1381 acttaatcga tggattggtg gtaaaaaatt aagtagttta tcatcagaag aactacaaaa
     1441 attccttttc attttaaaag aaaagggaat tgatggcgtt aatgttggtt atgctaaaaa
     1501 tagtttacaa tctcttgttc aggttctaaa tatggttttt aattattgta agaaaaagaa
     1561 tttgataaat gagaatccaa tgaagtttgt taaaatgcct aaatatcagt tatctgttaa
     1621 agatttaaaa gagtctgtca acagtttaga agataagttt ttaacagtgg atgaattacg
     1681 agctttttta aactatggga tagtttatga agagttaccc atgtctgttc tttttcatgt
     1741 tttgttttat actgggtgtc gtgtgagtga agcgcttgct ttacagcctc aagatataga
     1801 ttttgaacga aatgaaattt tgttttataa acagacagca gtaaaaggaa aaagtaaaga
     1861 ttttcgaata gaaactacaa aaactgttag ttctgcaaga agagtacccg tcacaccatt
     1921 agtaatggag aaattacaag aattaattga tgtattaaat aaaacaaaaa gaaatagtaa
     1981 ttttgttgta gatgaaactt atttatttgt ttatctcgat cctggaaagc gtggagttcc
     2041 ttatcgtcgt gaatatgtaa acgatcatgt taaacgttgt gtagagcgat gtggaattaa
     2101 caaagatttt cacacccatt tggctcgtca tactatggct agtttagtgg cagagtattg
     2161 cagttgggat gttttaaagg atcgtttagg tcatacagac agtacaacct caaaaatata
     2221 tcgccattta acatcaactg aaaagttaaa gcctctgact gcttttaatt ctttagaagg
     2281 ttaaaaagat ggtgaaagta ctggtattac aggattcttg aaaaacaatt ttttaatagg
     2341 ttagtcaaag tgtagtcaga ggcaataaaa attcttttat atcaatactg taaagacttt
     2401 tcacaagtat caaagaaatc cgtcgttaat cattgttatc agactgagaa agaacccttg
     2461 gtattcaagg gttctttttt cttagcatgt aatttgaaca ccctgatcca gttttttctt
     2521 taattattgg taggggagaa taatggatta gaataaatct acctataagt agtagtaaaa
     2581 aaaagagcac tatgataata tctttcttaa gagtaaccgc tttaataaaa aatccagaat
     2641 attctaacat aaatggagta ttcttagaat caaaaggaga aagaagatgg tttatactta
     2701 tacggagcaa gaaaagatga aagttttaga cacattcatg aatgatggaa aagtaaaaca
     2761 agtaccaagt tcaacaaaaa agaaatacat cctattgaaa gaagtcttga aacgctttga
     2821 ccatggagtg acttatacag aaacagaggt taattcgata ctgctgaacg tattttctag
     2881 tggcgattat gtcgaacaaa gacgttatct aatcacattt ggctttttca aaagaagttc
     2941 agacggtcgg gcatatcaga tgatgggaat tgaaaattag aagttttaat ttatactcat
     3001 tgaaaaaagt tcaagtatgc gatattggtt ctacaccgca tacttgaact ttttttgtca
     3061 tctgtatcta taatacaaag ccaagaacgt tattcaaaat atggatagct atgataaaag
     3121 ctaatgagcg attcgcagtt aaataagcaa tcgtcagtaa aatcgttcca ggagcgtaca
     3181 tgaaaaaatc aattggatgc gttacatgaa taaagataat tctcttttat ttgtacgaat
     3241 aaataccaaa a
//
LOCUS       DACDLI010000141         3199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDLI010000141 DACDLI010000000
VERSION     DACDLI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.146"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            301..585
                     /locus_tag="IV353_002701"
     CDS             301..585
                     /locus_tag="IV353_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010863.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="IV353_002702"
     CDS             588..1004
                     /locus_tag="IV353_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010864.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="IV353_002703"
     CDS             complement(1141..1860)
                     /locus_tag="IV353_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9010865.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="IV353_002704"
     CDS             2513..2608
                     /locus_tag="IV353_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9010866.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="IV353_002705"
     CDS             complement(2910..3155)
                     /locus_tag="IV353_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010867.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DACDLI010000142         3071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDLI010000142 DACDLI010000000
VERSION     DACDLI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.147"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..434
                     /locus_tag="IV353_002706"
     CDS             <1..434
                     /locus_tag="IV353_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9010868.1"
                     /translation="VKGKWHYLYRSIDSSGITLDIWLRKKRDKQAAYAFLKRLIKQFG
                     EPKVLVTDKAPSLSSAFNQLHSEGLFSKTEHRTNKYLNNIIEQDHRSIKKRHKLYQSI
                     RTASSTIKGIETIHALYKISQRDKSLFGFSVIHEMNTLMSI"
     gene            506..970
                     /locus_tag="IV353_002707"
                     /pseudo
     CDS             506..970
                     /locus_tag="IV353_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            970..1860
                     /locus_tag="IV353_002708"
     CDS             970..1860
                     /locus_tag="IV353_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9010869.1"
                     /translation="MEELIRVEHATKTFRSKNWRGTVSEVRAVEDVSLTIHRGETLGL
                     VGESGSGKTTLGRSILNLETLTSGEIFFEGKELTALTAEDRKAAYQQMQIIFQDPYSS
                     LNPRMTALELVMEPLYHETKDAARAKAKEILAIVGITGDDVHKLPRSFSGGQRQRIGI
                     ARAVVNRPAFVLCDEPTSALDVSIQAQIIQLLLRLQKEFSLSYLFISHDLSVIRHISD
                     RTAVMYQGKLVELAPTDRLFDNPLHPYTKKLLAAAPIADPVLARGAMEQTEIQSTITL
                     TPLYEWVEVEPDHFVRREKR"
     gene            1857..2249
                     /locus_tag="IV353_002709"
     CDS             1857..2249
                     /locus_tag="IV353_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAP9010870.1"
                     /translation="MTVETLTIHQGSDKLEMTTPTGNWVLAREKGYSPVQMLVSAVAA
                     CGGYVYASVLENSKITAEIEKIEVSYDRDDNKKAQPVSAIHIQFYAKIAEADHDKATR
                     CLKLVSPNCPVIQSLDPAIEVTETVTFI"
     gene            2435..3071
                     /locus_tag="IV353_002710"
                     /pseudo
     CDS             2435..3071
                     /locus_tag="IV353_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          975 a          613 c          674 g          809 t
ORIGIN      
        1 aggtaaaagg aaagtggcac tatttgtatc gatccattga ttcttcgggg ataacattag
       61 atatttggtt gcgaaaaaag cgtgataaac aagcagccta tgctttctta aaacgattga
      121 tcaaacaatt tggagaacct aaagttcttg tcactgataa agccccttca ctaagtagtg
      181 cgtttaacca attgcattct gaaggattat tttctaagac tgaacaccga acaaataaat
      241 acttgaataa tatcattgaa caggatcatc gatccatcaa gaaaaggcac aaattatatc
      301 agtccataag gactgcttcc tcaacaatta aaggaattga aaccatacac gctttatata
      361 aaattagcca aagagacaaa agtctctttg gcttttcagt gattcatgag atgaatacat
      421 taatgagtat ctaagcataa aatcaaaaat taattggaaa ctctttatta attttttaac
      481 tttgcaacag aacccttttc cagtacaacg tatgaaacaa tatccccacg agttatcagg
      541 agggatgcgt cagcgggtga tgatcgccat ggctttattg gcgcaaccga aattgctgat
      601 cgcagatgaa ccgacgacgg ctttagatgt gacgatccaa gcacagattt tggccttgat
      661 caaaaacctg caaagagaga accagctttc cgtcgtcctt gtgacccatg actttggggt
      721 cgttgcagga atgtgcgact ttattaaagt gatgtatcaa ggtcgagtcg tggaagaagg
      781 caccacggaa gagatctttt atcaacctgc gcatccttat acgaaacaat tattgcaagc
      841 ggcacattta ggcgacaagc atgccgaatt aaaagtcttt gactatcatg agacattggc
      901 aaatgagttg caagaaacgc agttatctaa gacccatcgc gtttggtgca atcaggaggt
      961 gacagaataa tggaagaact gattcgcgta gaacatgcga cgaaaacctt tcgcagcaaa
     1021 aactggcgcg gcactgtcag tgaagtccga gcggtcgaag atgtcagtct aacgatccat
     1081 cggggcgaga cattggggct agtaggggaa tctgggagtg ggaaaaccac tttaggacgc
     1141 agtattttaa atttagagac tttaacaagc ggagagattt tctttgaagg caaagagcta
     1201 acagcattga cggcagaaga tcgcaaagca gcgtatcagc aaatgcagat cattttccaa
     1261 gatccgtatt catcattgaa tccccgcatg acggcacttg aactggtgat ggagccgttg
     1321 tatcatgaaa cgaaagatgc tgcccgagcg aaagccaaag aaatcttagc gatcgtcggg
     1381 atcacgggtg atgatgtcca taaactgccc cgcagtttca gcggcggaca acggcaacgg
     1441 atcggcatcg ctcgtgcggt ggtcaatcgc ccggcgttcg tcttgtgtga tgaaccgact
     1501 tcggcattag atgtctccat tcaggctcag atcatccaat tgctgctgcg tttgcaaaaa
     1561 gagttttcat tgtcttattt gttcatttcc catgacttgt ccgtgattcg tcatatcagt
     1621 gaccgaacag ccgtcatgta tcaagggaaa ctagttgaat tggcacccac cgaccgtttg
     1681 tttgacaatc cgctgcatcc ttacacgaag aaattattag cagcagcgcc gatcgctgat
     1741 cctgtgttag caagaggtgc gatggaacaa acggagattc aaagcacgat tactttgaca
     1801 ccattgtatg aatgggtcga agttgaacca gatcattttg tgaggagaga aaaacgatga
     1861 cagtagaaac attgacgatc catcaaggaa gcgacaaact tgaaatgacg accccaactg
     1921 gcaattgggt gttagcacgg gaaaaaggct attcgccagt ccaaatgctg gtgagtgcgg
     1981 tcgctgcttg cggtggctat gtatacgcat ccgtcttaga gaattcaaaa atcacagcag
     2041 aaatcgaaaa gatcgaagtc agctatgacc gagacgacaa caaaaaagcc caaccagtca
     2101 gtgccatcca cattcagttt tatgcgaaaa tcgctgaagc cgaccatgac aaagcgaccc
     2161 gttgcctaaa actcgtttca ccaaattgcc ccgtgatcca atcattagat ccagcgatcg
     2221 aagtgacgga aacggtgacg tttatttaat cgaaaaagac tttgtgtgcc tgaaaccaat
     2281 aggcgtacaa agtcttttgg ctctataatt tatgggactg atgaatcaga attgattctt
     2341 cagccccatt ttcattttag gtattaaaaa acatatcgtt ctctagtatg attaaatcac
     2401 cacaaaataa acaactgaga ggacgatatg ctatatgaat gattctatca aaaaaatgct
     2461 gagaataata gagaaagatt tgatgattac agaggtctct tacgagaccc ttcagaagaa
     2521 aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta gaagttgtga
     2581 ttctactgtg gtagatggaa acgggaaagc aattatggtg aaaaatggga agaaggaaac
     2641 gattgtccgt tttgaacaat aaaatcatat gcctttggtt atgcgcctaa aaaagcagcg
     2701 ctatacctgt aaaaactgtc gcacccattg gactgctcaa agttattttg tccaacccag
     2761 acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg aaaaagtatc
     2821 tttatctttt attgcgaaaa gctgttaggt atctttgacc accgttattc gtacattgaa
     2881 agagtttaaa agctatttac caaagcaatc taagaagatt ctccccagag tattgatggt
     2941 tgatgaattt cgttcgcata cttctataga agataaaatg agctttattt gtgcagatgg
     3001 cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat taacaagcta
     3061 tttcttaggt t
//
LOCUS       DACDLI010000143         2646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDLI010000143 DACDLI010000000
VERSION     DACDLI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.149"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IV353_002711"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IV353_002711"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9010871.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVTNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          953 a          463 c          585 g          645 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ccgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta acgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaaaag
//
LOCUS       DACDLI010000144         2443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDLI010000144 DACDLI010000000
VERSION     DACDLI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.150"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..643)
                     /locus_tag="IV353_002712"
                     /pseudo
     CDS             complement(51..643)
                     /locus_tag="IV353_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            881..1303
                     /locus_tag="IV353_002713"
     CDS             881..1303
                     /locus_tag="IV353_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010872.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTLADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1313..1516
                     /locus_tag="IV353_002714"
     CDS             1313..1516
                     /locus_tag="IV353_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010873.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1727..2311
                     /locus_tag="IV353_002715"
     CDS             1727..2311
                     /locus_tag="IV353_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9010874.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          815 a          369 c          422 g          837 t
ORIGIN      
        1 attgtagttt tagatatctg agaataaaga gagctgtttc cttaccatac ttacaaatcg
       61 ttttttattc tataaactgt ttgtctagtg atgttataat tttttgcagt ttttgaaata
      121 gcaataccct gatttagatc ctcaacgatg cttttataaa caaggcgacg ttggggatct
      181 tttgtgtctg cgctgtataa cttaggacgt cctttataaa caccatttgc ttttgcaatc
      241 ttaattcctt gtgcctggcg tcgtttagat tctgtacgtt cctgttcggc aatcatggcc
      301 aggatctgaa tgattaaatc cttgatgaac ttatccaaga gaggattgct tcagccatga
      361 ttggtaaact agtgatgata agttgtacat tttttctttt tagataatta accgattcaa
      421 tgatctcgtc ataatttctt ccaagtcgat cgatggcttc cacgataaaa atatcttgtt
      481 ctcagacaaa ttctaaagct tcttggaaaa ttggtcgatg agtaatcgtt gctccagatt
      541 gtttctcaat aaatattttt tctgcgccga acttcttcat ttcttcaagt tgcctttgtt
      601 cattttgatc aatcgacgaa actcgaatat aagcaatttt caaaaattta ccctactttc
      661 tcgaactatt taaaacacac cctaatcata cattagaaaa ctagatgtca cttttctttt
      721 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
      781 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
      841 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
      901 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacacttgc
      961 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
     1021 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
     1081 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
     1141 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
     1201 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
     1261 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
     1321 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
     1381 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
     1441 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
     1501 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
     1561 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
     1621 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
     1681 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
     1741 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
     1801 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
     1861 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
     1921 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
     1981 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
     2041 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
     2101 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
     2161 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
     2221 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
     2281 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
     2341 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
     2401 tattctcaaa tgttgtaagg attttattca tttgttaata tta
//
LOCUS       DACDLI010000145         2421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDLI010000145 DACDLI010000000
VERSION     DACDLI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.151"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..452)
                     /locus_tag="IV353_002716"
     CDS             complement(<1..452)
                     /locus_tag="IV353_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP9010875.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRQKLAQMIQE"
     gene            901..1515
                     /locus_tag="IV353_002717"
     CDS             901..1515
                     /locus_tag="IV353_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9010876.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1539..1889)
                     /locus_tag="IV353_002718"
     CDS             complement(1539..1889)
                     /locus_tag="IV353_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9010877.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1883..2146)
                     /locus_tag="IV353_002719"
     CDS             complement(1883..2146)
                     /locus_tag="IV353_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9010878.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          801 a          434 c          376 g          810 t
ORIGIN      
        1 tgttcctgga tcatttgagc aagtttctgc ctgcgctgac ttcttgttcc ttcagtcgta
       61 aaaagattca gtgatctggt cacttttaag atcgattcat cgatcgaata gatcaataaa
      121 tcttcgtctg ctacatacct tctgaaaata ttgtttacct gcatgtttcg cttaatatag
      181 agattcatgc gtggtggaac gacatgtaac gttttaggaa aaggctgtgg tagatcccgt
      241 ggtctgctga cattagaaat accgtacttc ttctttgcag ctggtgaaga agctaaaatc
      301 aaccctgatc cagtgttgtc agcacgactc ataacaacaa gttctgttgt taatggatct
      361 aagtttcttt ctatacattc gacacttgcg taaaagcttt tcatatcgat taaaaagtaa
      421 tcatttactg gctcttttga ataatccatc ataaataaca ctccattctt tcacattata
      481 caaacatatg ttcgatattg taaagaagaa atagctaaat acataaaaaa gaagcatagg
      541 tatgctatca ccctgcttct tcttttgatt tgtatgatca atttattcta tcactttact
      601 aattaatttg aaaatctaat aaaaactctc actctaaaag agtgagagac cgcaaattag
      661 agtacggact tttttggagt tccgttcttt aatattaaca aatgaataaa atccttacaa
      721 catttgagaa taagttatca gtagacactt acaaaaaatt ctcatactat ttcctccatc
      781 attagttttt gtctatataa ggtataccct aactagacac gaccgcgaca ttgaaaaatc
      841 tggttttctc gtgtatgatt agggtgtttt ttaaatagtt gcagaaagta ggtaaaaatt
      901 ttgagagttg catacatacg tgtctcatcc atcgatcaaa atgaacaacg gcagattgaa
      961 gaaatgaaaa aattcggtgc agagagaatt ttcattgaga aacaatctgg cgcaacgatt
     1021 actcatcgac ctgtttttca agaagcactg gatttcgtcc gagatcaaga tatttttatt
     1081 gtggaagcta ttgatcgact tggaagaaat tacgacgaga tcatcgcttc agtcaattac
     1141 ttgaagaaga aaaatgtgca gctgatcatc acaagtctac cgatcatggc cgaagcaatt
     1201 ggcaatcctc tcctggataa attcattaaa gatttgatca ttcaaatttt ggccatgatc
     1261 gctgaacaag aacgtaccga atctaaacga cgtcaggcac aaggcattaa gattgcaaaa
     1321 gcgaacggtg tttataaagg acgtcctaag ctatacagtg ccaatgcaaa agatccccaa
     1381 cgtcgtcttg tttataaaaa catcgttgag gatctaaaaa aaggtgttgc gatcgctaag
     1441 attgcaaaag actacaatgt tacgagacaa accgtatata gaataaaaaa agacagcatg
     1501 gtcaatcacg aatagttgat catgctgctg tttctatttt atttgcttac tttttgaaaa
     1561 actgttctaa ctttcatcaa taactcctca actaaactat catgcgcttt ttccaaaaaa
     1621 atacactctc tggcttcata atcaattgtc gttagttgat ctaataatac ttttcctgtt
     1681 gtcttcattt catattctga gtttatattt acatatagcg gatagtttct agttgtacta
     1741 gaaattggag caactgtaac aacgttacta taatccgcaa taatatcatg acttaaaaca
     1801 atataaggtc ttttacctgt ctgttcgtgt ccagatcgag gtgctgtatt caataataga
     1861 atatcgcctt gtctaggttt tctcaccatt tttcattacc tactgcttca cctaaatttt
     1921 gaatcgtcac atgatttctt tcatcagtgt aattttcaaa taactgttca attgttaatt
     1981 cattttctat attagccgct tcgataatta gccgcttctg accgtttact tcttcttgta
     2041 ttatcttcac tttgtcttct gtatgaattc caagatattc cataatatac tttggcaatc
     2101 taaggccatt actatttccc cattttttta taggtaacat ttccatagta gacacctctt
     2161 tctgtatata catagtataa catacataag aaaaaacgta tatacttaat tatccttttc
     2221 tattaaatta gattaacatt ccatgaatcc ataaactatg ttaacattta tatgaaaata
     2281 gtcgaaagga aatcataata tgtttaagaa aacaaaatct ttagaatcag gtaagaaaaa
     2341 aattgatcta aaaataatct cttctggata ctttaaagat ttcatagtta ttgatacaaa
     2401 atggaataaa aaggacatag a
//
LOCUS       DACDLI010000146         2357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDLI010000146 DACDLI010000000
VERSION     DACDLI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.152"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(118..708)
                     /locus_tag="IV353_002720"
     CDS             complement(118..708)
                     /locus_tag="IV353_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9010879.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="IV353_002721"
     CDS             1231..1392
                     /locus_tag="IV353_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010880.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="IV353_002722"
     CDS             1411..1905
                     /locus_tag="IV353_002722"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP9010881.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          842 a          361 c          392 g          762 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttt
//
LOCUS       DACDLI010000147         2337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDLI010000147 DACDLI010000000
VERSION     DACDLI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.153"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(184..537)
                     /locus_tag="IV353_002723"
     CDS             complement(184..537)
                     /locus_tag="IV353_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010882.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
     gene            complement(1169..>2337)
                     /locus_tag="IV353_002724"
     CDS             complement(1169..>2337)
                     /locus_tag="IV353_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9010883.1"
                     /translation="IVIKRMRRKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFP
                     FLLGVWGYFNRITIGRFFEQNTFSKIWLLLLASLCLILFPIFPYTLNELLISICFALL
                     LASVDDMNIAKKTKIASTKLSFVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGL
                     FTVFLCTLLMITCFRKGVQKMRSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQS
                     LLKENSSKANAGENLLSQEKKILFIGDSVMLGAYQELKETFKKEALVDAKESRQITAL
                     PEILKEHKDLVSYQQIVIGLGTNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTI
                     NATLATLPNQYSNITMIDWYSESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT          764 a          422 c          309 g          842 t
ORIGIN      
        1 tgatgcatca tgaattaaca gtgcattatc catcttttcc atgttaaaag aactaagaat
       61 caagataacg tcttgattct tagttctttt attccttcta gaaaataatt gttagcaata
      121 tagtttatat ttgtaaatac taaatcagta gcttctagtt gctttcctgc ttaatagttc
      181 atattactca atgtaaaaaa taattgattg tggacattgc tcatccactt tgtatccaca
      241 actttcttta taataggtat gataatcact ctctaccata ttaattaact cttttccccc
      301 gcaaaattct tcaatgtcta aattaataaa ttcctctaaa agggaaagct tagagtcaat
      361 cgaaagcatt ttaggtaaca tagtccaaaa gtcattatca tcgtttttac gtagtgtata
      421 atatgtttgc ttttctagat ttaatacata attatatgca tcataatctc cctttttttc
      481 aaaaaaattt ttcatattta aaaatctact cacaaatttt ttcttgaaac tattcatttt
      541 ctcacctcta tttattcttt ttcttgtcca tagcataact tttgaataga gtaactaaat
      601 tcaaaagcta ttatagtata aaaatacact ttttgttgtt caaatttttc atctaattgt
      661 gcagtaattt tgaactctat attttttaat atttaatgct taagcaatca tttataaaaa
      721 aagactggac gataattaaa ataacaaaaa aggctgtgac acgtcttttg tcacagtccc
      781 ttctagcatt atattactat tatttattca ctgcatcttc ttaagagtaa aacatacttg
      841 atcgtttgtt tctttactaa cagccttctt tacttggttc ctacgatcct taattttatc
      901 taagctgatt gctgcttgta accataaaac ttcgatcaga acatacatcc tagtattgtc
      961 gtctcgctca tgttctgtat aacaaagagg ataaatccaa agcgatttct ttccaagaag
     1021 ctctcagtta acgacctcgc ccatctcttt tttaccacga atattaaaaa gctcctcctc
     1081 ttaaacaccg tttatcatat gaagtatttt ttatttaaat tgattcacct attaggaaaa
     1141 agtaaggtta acacacgtaa tcactacctt attgaataac tgaagcaata tactttgcat
     1201 aagctgtacg tccactttca ttcaaatggg tttcatcatc ataaaaaaat tcaggatgat
     1261 ttttgctctc actataccaa tcgatcatcg ttatattaga atactgattt ggcaatgtag
     1321 ctaatgtggc atttatcgta tcttgccagc cagttggtgc ttttatattt acccagtata
     1381 cttttttatc tttcagcaaa gacattgttt gttctatagc ttcatctgtt atcacaccat
     1441 tggttcctaa cccgatcact atttgttggt aagaaactaa atctttatgc tcctttaaga
     1501 tttctggtaa cgcagtgatt tgtcttgatt cttttgcatc taccaatgct tcttttttaa
     1561 atgtctcctt tagttcctga tacgccccca gcataacaga atcaccgata aataatattt
     1621 tcttctcttg tgacaacaga ttttctcctg catttgcttt actagagttt tcttttaata
     1681 aactttgcaa ctgattttgg tcagcggtca gtcgactggc aggagcacgt aataccagta
     1741 cagaaactcc gattatcatt agtgccatag ctgtccaaat tttgggtgat ctcatctttt
     1801 gcactccttt tctaaagcaa gtaatcatga gtaatgtaca taaaaacaca gtgaatagac
     1861 ctgcgatatc caaatatttc caatggtgat gtttgaatag atttgtcaga gcaatcaata
     1921 ctgggaaatg caccaaataa agttcataac tgatttttcc tacaaaagaa agttttgtac
     1981 tagcgatttt ggtctttttt gcaatgttca tatcatcgac actggctaac aatagagcaa
     2041 aacaaatact gatcagtagt tcatttagtg tataaggaaa aatcgggaac agaatcagac
     2101 aaagactagc tagtaataaa agccagattt tactaaaagt gttttgttca aagaaacggc
     2161 caatagttat tcgattaaaa tacccccaaa ctcctaataa aaatggaaaa agtttagcta
     2221 tcgtatgata atatgcctta gttgaaggta aaaacaaact aaaaagaata gaaacaaaaa
     2281 tcaatgtata gattaagtaa aacacttgtt ttcttctcat acgcttaatg acaataa
//
LOCUS       DACDLI010000148         2333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDLI010000148 DACDLI010000000
VERSION     DACDLI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.154"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..2186
                     /locus_tag="IV353_002725"
     CDS             33..2186
                     /locus_tag="IV353_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9010884.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          802 a          415 c          522 g          594 t
ORIGIN      
        1 agcatgaatg taaataggcg gaaggatagg aaatgatgaa agattttatg aaaaaaatat
       61 taaatacttc ttggttagaa aaaataagaa aaggtgcaca agaacctaag aacaaaacgt
      121 ttcgccggtc ccatgttcct tttcgcttaa attttctttt tttcattata tttacgttat
      181 ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt gaacggcgaa gagatggagg
      241 cgcgagtgaa gtctacttct acgattacgg tccaagaaag tactcctcgc ggaatgatct
      301 acgactccac aggaaaagct ttagttacga ataaagcgaa tcaggccatc acatatacga
      361 gaggaacaca agttacggct aaagatttgc ttgaaatcgc aacgaagctg aactcgctga
      421 tcgatgttcc ggtagatgaa aatctgacag atagagataa aaaagatttt tggctggctg
      481 atcccaaaca tttgaaagaa gccacaaaaa gactttcggc aaaagaaaag caattaggga
      541 acagcgaaca atatagtgcg atggtcaata aagtcacagc tgatgagatt aatttaaacg
      601 aggagcaatt aaaaattgct accatcttta aacggatgaa tagtgcatat gaattaaaca
      661 ctgtctatat caaaaactct ggtgttactg atcaagagtt agctgttgtg gcagaacgcg
      721 cttctgaact tccaggagtt tcgactggca cagactggac acgagaatat aatgcagcaa
      781 gttcactgaa aagtattctt ggcagtgtga cgactgaaaa gcaaggactt ccagcagatg
      841 aagcagagaa atacctagca aaaggttatt ctcggaatga ccgtgtagga caaagttatt
      901 tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac ccaatatgaa gtttcccttg
      961 ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc tggggaaaaa ggttctaatt
     1021 taatgttatc tatgaatgca gaattccaat caaaagtaga agaaattttg aaaaggaatt
     1081 atcaaacatt gatcaacaac ggcaaagcac agtattcgcc tggtgcttat gcagtggcta
     1141 tgaatcctca gacaggagaa gtgctagcaa tgactggttt cagtcatgaa caaggttcca
     1201 aagaaatcac cgagaatgct ttaggcacga ttactagcgc ctttgatcca gggtctgtag
     1261 tcaaggctgg tactttgaca gcaggctggg caagtaacgc gatcagcgga aatcaagttt
     1321 tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa aagctctgtc tttaatcgaa
     1381 gcggacaagt tgctcttgat gcagtcaaag catcggaatt gtcttctaat acgtatatga
     1441 tcaaagtggc gttaaagatg ttaggactag actatacacc tggaatggga ctacctagtt
     1501 tagatgaaga agctaaagct tatcagcaat tgagagactc tttcaaagag tttggtcttg
     1561 gtacaacgac tgggatcgat ttgccgaatg aatctcctgg gatctcacgt tctgttgatt
     1621 atatgaagaa gttcaatgcg gataatggca aagaatggta tgctccaggt aactttaccg
     1681 atttggcatt tggacagttt gacacatata caccaatcca attagctcaa tatgcttcaa
     1741 cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt caaagcgatc tatggcaatg
     1801 acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg cacaacagta gaaaatacgg
     1861 tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg attccaccaa gttgtccatg
     1921 gtaccgatcc ttacacaaca gccaaaccat tagccagtgc gaaaatggac ctttccgcaa
     1981 aaacagggac tgcagaaacg gttgcggaag gtcatccaga tattgcgaca gtcaatagta
     2041 atatcgtggc atacggacca acagacaatc cggaaattgc catcagtgtt gtcttgccga
     2101 acttattaga cgaacaagac cacataaact tgacgattgc taaagaaata atggacgcgt
     2161 attatgatat gttcatggca aaataaaaaa gaggtcgtga aaaagccgct tagtttcgag
     2221 tatgaagaaa aattttgtga aaatagcttc tcacattttt gcttaaattt gacttcatac
     2281 cgagaaactg gcttttgaac accgtttact cggcaaaaaa ggttgtgaca aaa
//
LOCUS       DACDLI010000149         2260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDLI010000149 DACDLI010000000
VERSION     DACDLI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.155"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..356)
                     /locus_tag="IV353_002726"
     CDS             complement(<1..356)
                     /locus_tag="IV353_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010885.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIK"
     gene            complement(357..1214)
                     /locus_tag="IV353_002727"
     CDS             complement(357..1214)
                     /locus_tag="IV353_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9010886.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1499..1786)
                     /locus_tag="IV353_002728"
     CDS             complement(1499..1786)
                     /locus_tag="IV353_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9010887.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(1776..2105)
                     /locus_tag="IV353_002729"
     CDS             complement(1776..2105)
                     /locus_tag="IV353_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9010888.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          666 a          452 c          303 g          839 t
ORIGIN      
        1 tgtttaatat actcatctaa agcttttcta agcaattcat ctcttgtcat attagtctca
       61 tacttttcta ctaaaactct ggaagtcgca tttaacaaag tcgacagttt taaaggatgc
      121 ttggtggaat aaaccttatc aaactttctg attggtgcgc caactttttt tgttcttcct
      181 ctactctgtc tttcaacatt ggaggaagtt tttatttctt gaacttggtg cacttctaat
      241 tgcttatttt cagatggtgt aattgcttgc tgctttttct ttgcttctgc ttcttttttt
      301 attttggcga gtcctttgtt gttcccaaaa atatcaagtc ctttgatgtc agccaattac
      361 tccacctccg cataccaatt ctcgtgttct tccaattctt tcaaaatact gataaataat
      421 tcgtggcttt tgcgatccca ttgttttaag agacctttct ttgtcttctc catcgtaata
      481 cctgatccgt cgtatcgttt taagcgcgca tttttgagta caacattttt aatcaaatgt
      541 tccccataaa tctcttgggc ttgtttatac atttcctgat ctactgcgtc accgctttcc
      601 aacatgaaag gaataacacc agcaacatat aaatttgtat caaattcttt cacaaaaaag
      661 tccatatact caagatattg catcgcattt ctcatcgatt ttacttgggt ctgtaagacg
      721 acgattgccc agtccgcaat gtacatcgcg gatttactat atacagaaat agaaggtggg
      781 acatctaaat agatgacgtc aaactcatca aataatggtt ctagcttttc tttcatatat
      841 ttgaattggg acaatgaatc attatattgg tctttgatcc aatcatcata catttcaaag
      901 gacatgtctg ctggaagtat atagaggtta ttgattactt tatagacagc ctctttaggt
      961 ggaagattgt ttatcaacac gtcagtcaca gtattttcgt aaacaatcgg ttcaccttct
     1021 ccgcctcctt cttcgttagt gaagttcata tgcgttaagt tcatcacatc agaagtattt
     1081 ccttgcatat ccaagtcaat tatcaaggtt ctttttcctt cattaactgc tgattcaaac
     1141 cctaacattt gcgcgcacgt acttttacct acgccacctt taaaattacc gattacatat
     1201 ttcttcgcta ccacgttcct acctcttttc ttttagcaat attcatttga tactattata
     1261 acatatttat ttttagttac taccaaaaaa aagatatata tgtactacct tataattaat
     1321 aagtaaaagg tagtacataa ataaggaaaa gctgaaatat attgatttat agctttcctc
     1381 aaagtaatga cgttatttaa tttatcgaga agcagagaat actcgcgact ttagtcatgt
     1441 agacgaatca gattttcgat aaaaagaagc cgtccttctt aagaacgaca acaaatcact
     1501 agattaattt tttcaactgt tcataaaagt tttcatgcgt tccacataac aatactggaa
     1561 taaccttgtt ttctataatc tcgtaagcta ctctatatgt tgtgcccgca tatttgaacc
     1621 cactagacca aatacctgat aaatccccag ttttttgttc acccttatat ggatcaatcg
     1681 caatttcatc ataaattaaa ttcaaaaatt tttctttcaa tggcttctct tttagctttt
     1741 ttaaatactt aatgacatgt tttgaaggaa cgatttcata agccaatttc tgttgccaac
     1801 tcctctttag tcattacttt agaattagta agtgtatctt cagcaatatc tctaaaggaa
     1861 gaatgcattt tgttttttct attcttaaat tctacaagaa tctcatcttt atcgtagcct
     1921 tctgcaatta tatcagacaa aatatcttcg ctaaaatcat aaaattcttt ttgtggttca
     1981 acaaattcat aaagaatacc attctctacc aactttgctt caacttcgac tccattagga
     2041 acattaaaat ctttaggtac tgtcaacatg attgaattgc cttgttttct tgtttttgta
     2101 atcatttaaa tccctcattt ctaacagtat gtaattactt agtaaataca ttataccacg
     2161 agataatgta tttacaagta attactgtga ctcgtaatgc gtccttgctg aataaatttc
     2221 aaccacgtgt tcatcttcat ctaccttata aacaacccgg
//
LOCUS       DACDLI010000150         2220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDLI010000150 DACDLI010000000
VERSION     DACDLI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.156"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..1598)
                     /locus_tag="IV353_002730"
     CDS             complement(51..1598)
                     /locus_tag="IV353_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9010889.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IV353_002731"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IV353_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9010890.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          622 a          465 c          397 g          736 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
//
LOCUS       DACDLI010000151         2056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDLI010000151 DACDLI010000000
VERSION     DACDLI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.159"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IV353_002732"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IV353_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9010891.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IV353_002733"
     CDS             852..1040
                     /locus_tag="IV353_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9010892.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IV353_002734"
     CDS             1053..1610
                     /locus_tag="IV353_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9010893.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IV353_002735"
     CDS             1642..1884
                     /locus_tag="IV353_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9010894.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          761 a          313 c          413 g          569 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagac
//
LOCUS       DACDLI010000152         2055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDLI010000152 DACDLI010000000
VERSION     DACDLI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.160"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..634)
                     /locus_tag="IV353_002736"
     CDS             complement(62..634)
                     /locus_tag="IV353_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9010895.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(650..1255)
                     /locus_tag="IV353_002737"
     CDS             complement(650..1255)
                     /locus_tag="IV353_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9010896.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(1342..>2055)
                     /locus_tag="IV353_002738"
     CDS             complement(1342..>2055)
                     /locus_tag="IV353_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9010897.1"
                     /translation="KEYVQKRVVQGWTPDVIVGRSEMKLDCSVRTLYRMFQTGAFNVR
                     DLPMKGKRKPNGHQEKRGKQAFRRSIHERIKEHPEFENEFGHLEGDTIVGRKHKSAVI
                     TLVERLSKVIITLKPAGRQAMDIENRINHWFQTVPKHLFKSITFDCGKEFSNWKAMGN
                     QNDIDIYFADPGTPSQRALNEHSNGLLRRDGLVKAMDFNEVSESFIEYRKHFLYSILS
                     SFKFSIDYIYCEFCRGSET"
BASE COUNT          576 a          450 c          309 g          720 t
ORIGIN      
        1 aatagactca tatctagaac gcattaagta tttacctgtc tggttcgctg agacttcttt
       61 tttaactttc ttcaattttc ttctttattc taaaaagcgt actgcgactg aaaccggtta
      121 tagcttcaac ttccttatac gatttacctg aagtaagcag ttcatatgcg tgcctcttct
      181 tcggtgtgat tgttgctttc ggacgtcctt ctctgaaatt aggattattc tttcttgcaa
      241 acatttttcc ttcttgggta cgttccacaa tcatatcacg ttccatttct gctacactca
      301 acaacgtccg tgtcaccatt cgccccatgg acgtattttc aatcgttcct agattcaaaa
      361 ccttgatcgt tacgttatca tcgagtaaag gttcaatgat ctctaatgct tctctcgtgt
      421 tacgagcaaa tcgatcaagc ttcgtaacaa ctatttcatc tccaggcttc agaccagcca
      481 acagctgatt gaattttggc cgatcccgtt ttgtacccgt aaatttttct gaaaaaatat
      541 tcttcttctc tacgccgttc tcaagcaact gttgaatttg ttcttttgta ctttgagaag
      601 cggttgagac acgtgcatat ccatatttcg tcatttggtc cacctacttt tacaaatcat
      661 ctatcgtata ctcagaatac ccttcataga aatagctgac atcgattcct ttcatatata
      721 actcgcgatc gtcaatttta tcagtcagag cattggaaat tagatagcgt atttccaaat
      781 cgtttatggg acttcgctcc attgcagaaa gatagtctgc tttatcgatt aagttccaat
      841 ctacaacttt ttgcaattct tcctttaaaa tgagatccag ccaaattcta gtacttcggc
      901 catttccttc tcgaaatggg tgagcgatat tcatctccac atatttcttg ataatctctt
      961 caaaagaagt ttgtggcatc tgatcaatat gctgtaacga atgttctaaa tacataactg
     1021 gtgcaaatcg aaaatttcct tttgcaatat tgaccgatcg gatttttccg gcaaaatcgt
     1081 aaatatccga aaatagaaat tcatgaattt cagataaacc tttaaatgta ccaacttcga
     1141 tttcatttat ttttccacaa tcgtacaatt ctttcgcttt ttgtttactg agtttttctt
     1201 caaccttagc caactcaact tgattggtta atcccaattt attctctaaa atcatgcgaa
     1261 atctccttaa ctttatgaca taagtttatg aaacactctc aaccccatta tatcgcgatt
     1321 ttgtttatgt gtcaaaaact attaagtttc tgaccctcta caaaattcac aatagatata
     1381 atctattgaa aatttaaaag aagacaaaat agagtacaga aaatgttttc tgtactctat
     1441 aaatgattct gatacttcat tgaagtccat ggctttaact aatccatcac gacgtaataa
     1501 tccatttgaa tgttcattta gagcgcgttg tgacggggtg cctggatccg caaaataaat
     1561 atcaatatca ttttgatttc ccatagcttt ccaatttgaa aattctttcc cacagtcaaa
     1621 cgtaattgat ttaaacagat gtttaggaac cgtttgaaac caatgattaa ttcgattttc
     1681 aatgtccatt gcctgtctac cggctggctt caatgtaata atcacttttg acaaacgttc
     1741 caccaatgta ataacagcac ttttgtgttt tcgaccgaca atagtgtctc cttcaagatg
     1801 gccaaactcg ttttcaaatt ctggatgctc cttgatgcgc tcatgtatac tgcgccgaaa
     1861 agcttgtttc ccacgttttt cttggtgtcc atttggtttt cgtttgcctt tcataggcaa
     1921 atcacgcaca ttaaatgcgc cagtttgaaa cattcgatac agtgtacgga ctgaacaatc
     1981 aagtttcatt tccgagcggc cgacaatcac atccggtgtc cacccttgaa caaccctctt
     2041 ttgaacatat tcttt
//
LOCUS       DACDLI010000153         2051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDLI010000153 DACDLI010000000
VERSION     DACDLI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.161"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            948..1421
                     /locus_tag="IV353_002739"
     CDS             948..1421
                     /locus_tag="IV353_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9010898.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="IV353_002740"
     CDS             1430..1657
                     /locus_tag="IV353_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9010899.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          666 a          333 c          305 g          747 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata t
//
LOCUS       DACDLI010000154         1993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDLI010000154 DACDLI010000000
VERSION     DACDLI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.162"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..903
                     /locus_tag="IV353_002741"
     CDS             <1..903
                     /locus_tag="IV353_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAP9010900.1"
                     /translation="KKNNLNLINAVERYLDVKRNGLLFSKGVILVEGDAEEILIPQIV
                     KKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTDLDEQAIPSEHRLYK
                     STAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKENILTYVSPIIDDAFK
                     DKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILNAI
                     AFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYFGK
                     DEDVVYLINQIENYTKILYAEVSK"
     gene            900..>1993
                     /locus_tag="IV353_002742"
     CDS             900..>1993
                     /locus_tag="IV353_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9010901.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPY"
BASE COUNT          767 a          253 c          346 g          627 t
ORIGIN      
        1 aaaaagaaca atctaaatct aataaacgca gtagagagat atttagatgt taaaagaaat
       61 ggattgcttt tttctaaagg tgttatctta gttgaaggag atgctgaaga aatacttatt
      121 cctcaaatag taaaaaaatt atttggtata aacttagacg aacttggaat aggtttgatt
      181 aatgttggta gtacttcgtt tgaatatatc gctagcttgt ttgacgatga tagaatacag
      241 agaaagtgtg ctattgtaac tgatttagat gaacaggcaa tcccatcaga acatcgattg
      301 tataaaagca ctgcacaaga gaaaggtcaa gcaagaaaag aaaaattatc ctcccttttt
      361 aatgaaaaca attgggttaa ttgtttttat gctaatacaa catttgaaat tgagttttta
      421 gatgtgaata aagaaaatat tctaacttat gtcagtccaa ttatagatga cgcattcaaa
      481 gataaaaaaa caataactgc ttataaagaa gaaatacaaa gtgaacaata tttagaaaga
      541 aatttagcga tgttaaaact agccaatcat gttggtaagg ggtggtttgc aattgagtta
      601 ggtgaaaaaa ttgacttttt aatcaaaatt cctaagtata ttcttaatgc tattgctttt
      661 gcatgtcagg aaatagtaga tctctcaatt tataaaaaaa ttctactcca tacaatcgac
      721 ttgtacgagg aaactacaga attgtcagct ttatcagcta gtttaaaaac aagtgataca
      781 gctgatttga atatagcaat aatttcttta ttagagtact ttggtaaaga tgaggatgtt
      841 gtgtatctaa tcaatcagat agaaaattac acaaaaatac tttatgctga ggtttcaaaa
      901 tgacaaatat cttcgaaaaa atcgtaaatg atttaaattt agaacaaaaa gatgttgtgg
      961 aaagtgaaga taacctttat gtaaatgcat gtcctgggag tgggaaaaca agggtactta
     1021 caaggaaaat tgcttaccaa gcaattaaac acgatggatc actaaaaaga ataattgcta
     1081 tcacatacac taatagagct gctgaagaaa ttaaggaacg cctaagttta cttagtatag
     1141 atgatgatat taatatttgg gtggggacaa tacatcagtt ttgtcttgaa tttattattt
     1201 atccttttaa aatgaatctc tcaagagtat caaaaggatt tacaattatt gatgattata
     1261 ctcaaagagc ttacgtcagc caaattaata atatgctgaa tttaaatctc aaaacgtatg
     1321 aactgaacaa aattgatttg tcactttctc atgattttaa aattaatgaa gttcagtata
     1381 ctcaagtttc tgaaatgtat cacactatgt tggaaaaaaa taaagaaatt gattttgata
     1441 tgattttact agtagcgtat aaaattttaa ctgtaaacag taacgttgca cgtaatattg
     1501 ctagtaatat aagggcgatt tatgtggatg aatttcaaga tactagagaa ttacaataca
     1561 atgtaatagc taaattactt caaaacaacc ctcaaattca gtcaatgttt gttggagata
     1621 ttgaccaagc aatttatgga tcattagatg gtatagctaa atcagttgaa gaactagaaa
     1681 tgctaactag tcacactttt caatcaaaga cgctacatgg ctgttacagg tctaatcaga
     1741 gattagtaga cttctactct aattttcaga gttgtcctta cgagattgaa agcagaggga
     1801 ataataaaaa cgaccgtggt tttataagtt atgattgcaa agttactaag gaggatttac
     1861 ccaacataat aaaaaatata ataaaggaaa agattgaaag cggcattcat gaaaaagata
     1921 tttgtgtact tgctcctcaa cactatcctc tgttttcact gggtgagtat ttaaagaaag
     1981 aattaccgta ttg
//
LOCUS       DACDLI010000155         1938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDLI010000155 DACDLI010000000
VERSION     DACDLI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.163"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..687)
                     /locus_tag="IV353_002743"
     CDS             complement(<1..687)
                     /locus_tag="IV353_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010902.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGG"
     gene            complement(716..1102)
                     /locus_tag="IV353_002744"
     CDS             complement(716..1102)
                     /locus_tag="IV353_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9010903.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(1118..1432)
                     /locus_tag="IV353_002745"
     CDS             complement(1118..1432)
                     /locus_tag="IV353_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9010904.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(1756..>1938)
                     /locus_tag="IV353_002746"
     CDS             complement(1756..>1938)
                     /locus_tag="IV353_002746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010905.1"
                     /translation="KATNITIITSLVGYGLLAISILSIDYGVTILQVLVLAIPMYLSL
                     FIMHEIYKLFVLSKIA"
BASE COUNT          551 a          391 c          337 g          659 t
ORIGIN      
        1 accaccagca atcaacatat gagggagctt atcatattcc caccatacgt ttttcattaa
       61 gcgaagttta ccatctttag cttctacttc atcaatagaa atacgactgg ctatggtgtc
      121 atagagcaaa gtatattcca cataggaatc ctttaactct ttatccgtca gctcacagta
      181 caagccactc tctaatttct tttccaagtg taagagttgg tcttgatatt ttcccagcgt
      241 gatttccacc cgtatctgta tcaagccatt tttaagtcga taatacattt tagggaagta
      301 ggttatcttt tcctttgtac gaccagcact atctttaaag aaaccctctg ttttgacctg
      361 ttcagattca taccacttgt tttcaagtat catctttgcc agtttttgac ggtggtaaag
      421 ttgtttaacc gtatcatagc gaacccgttt gaatacaaac gctaccagca agcagataag
      481 aattgcgaca ctgaaactga taattaaata gggaatgtca atcttatctg cttgtgatag
      541 gttaaaatcc tgccagttga tctgctggat tgtcttcaca tgaaacagtc cgacaaccag
      601 caggaaaaca ggcaggagtg acgctatcgt aaaatgaaag actaaatctt taccagatgg
      661 gcgaatcctt ttaccacgct gtttcatgcg aaaaagtctc ctttctacct agcgactatt
      721 tgtcttgtgt cggttctttc tttgcttgtg gttgagcttt gaatgaacta gaatcctttg
      781 tcagcacaat atcgtctgcc ttgatatacc agtcaacatc tgctccttga taggtggcag
      841 tagcaacggt gtccgcaatg ggattgataa gttccacccg tgcgttataa tcaaactctt
      901 tcaaaggcac gctggcagga atacttactt gaatcatgcg tccttgtcct ttggatttta
      961 agtcataggt acgttccttg atttcatctg aaaccgaccc gtcttcattt tggattctca
     1021 cttcacgacg tagagcagag aatttcaatt ctccaaaagt cgtgtcttta tctaatacaa
     1081 tgccatttgc taatctcatc atttttcctc tctttcttta ttcttttatc atgtcgtcag
     1141 catgtaaaag gtaatttgta aaaccacgag tgccgatttt gtagccctct gcggtaatac
     1201 gtggattgac taacttcaca cgttcctcaa agccgaaatg tttttcgcca gcttcagcag
     1261 gaagcaccac cacaatatca tctgctcttt gaacatcaga atagagatta tagcttcttg
     1321 ataagacagt tagccgtccg ttgattcttc gctgaacgac tttatcctcg ccagcaaatt
     1381 ctaaattgcc gaatgttttt tccatgttgg gaatcacaaa tttaagttcc atatttttac
     1441 ctatcctttc ttttttattg gctgaatgaa tgtttgatgg tcttaaagag tggggaacga
     1501 ccttttgatt cttgattttt tgttttcata agttcacttc ctttcaaaat cgggtaaaaa
     1561 aatagacacc tcattttttg aagtgtctac ctattaaata ttcaaatttt attggaagta
     1621 tctttatatc ttcacttttc aaggataaat cgtcgtatca aagctcattc ataagtagta
     1681 aattagtagt aaattgagtg gttttgacct tgataaagtg tgataagtcc agtttttatg
     1741 cggataacta gatttttatg ctatttttga taaaacaaat agcttatata tttcatgcat
     1801 gataaacaat gatagataca taggtatagc caaaactaag acttgcagta tagttactcc
     1861 ataatctata gacaaaatac tgattgctag aagtccatag cctaccagag aagtaatgat
     1921 cgttatgttg gtagcctt
//
LOCUS       DACDLI010000156         1757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDLI010000156 DACDLI010000000
VERSION     DACDLI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.164"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..601)
                     /locus_tag="IV353_002747"
                     /pseudo
     CDS             complement(1..601)
                     /locus_tag="IV353_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            679..1718
                     /locus_tag="IV353_002748"
                     /pseudo
     CDS             679..1718
                     /locus_tag="IV353_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          502 a          385 c          374 g          496 t
ORIGIN      
        1 ataaattggg gtgtgcagga gaaatttccc cagcatacga agaatacttt tttcgtctag
       61 aaaatttaac aaatagattt tcctcagtca tcaatctacg tacaactttt tcagaaagct
      121 ttttacccat attttttaaa gcttggtcaa ttctacggta accatacgca cagaagttat
      181 gattgaaaat cgtgataatt aattgacgtt ctttgtaata cttatctggt ttctcaagtg
      241 cttgtttttg ataaaaatag ctgcttttag gcaactggag ctgttgaaga agctctttca
      301 aggtgaactg atttcgtaag gcatcaatca atatcgtttt ttcttgattt gatagacaaa
      361 gaagattgac gcctttttct ttttttagca gttcagccgc tccttcgagt aatgcttttt
      421 cgagctgtaa ttggtgtact tgtttttgta agtcttctac ttcctttaaa agtagctctt
      481 tttgtttttc ctgatttttt aaagtcatac gcaatggaaa atcctttcca agaaccgtgt
      541 ttttccattg atatagagta actctactaa ttccttcaga ttcggctaaa tttttcctat
      601 tatataaatt gagttaaatt gtattcgttt ttcctcctat aggcgtatac taaagaaaac
      661 acttgaggag tggggaacat ggagacagaa cgccaaatcc gtgaattgga aaaagcgatg
      721 aatacgacag acaaccgccg gttgtacgag cgtttcctgg cagtcaagtt ggttcttgaa
      781 ggccacaccc ggaaagaagc cgggcacacc atcggccgcg acgagcatac tgtcagccac
      841 tatgtgaaaa actactgcca taatggaatc caaggtttag tctccaaaaa acaaagcgga
      901 cgaccttcac ggctgacaga tgaacaggaa gcggaactgg tccaaatcgt cgcctaccac
      961 actccgaagg aagtaggttt caaatcccgt gccaattgga cattggcgat tgtgtgtgac
     1021 ctgattctcc gggaatggag ttatggatat acgcaaaaag gcgtggcgga cctccttcac
     1081 cggctaggac taagctggac ccgtccgacg tataccctca aaaaagcgga ttccaagaaa
     1141 caacaggtat ttttggaaga aaccttcccg agcctaaaaa aaaattgctg aatagcgata
     1201 ttgaccacct cctattcgag gacgaaacca tgatccggga ttaccaagcc attggctgca
     1261 cctggtttct ccgcggccac cagcgaaaaa tccccactta tggacaacac aaaggggtaa
     1321 aattgatagg caccttggat tatgaaaccg gtcggatcat ttgtacagaa gaaggggcat
     1381 acgacgcagc ggccttcctc cgttttctgg agcggacgtt gcgggaatac cccaccggaa
     1441 aaatcgtgat ggtattggat aatgccctta tccaccacgc caagctgatc cagtcttttc
     1501 ttgaagaaaa cgctaggcgc ctggcactca tcttcctccc tccctacaga cctcagttga
     1561 acctaatgga aggcgtttgg aagtgactca aggaatcggt catcaacaat gttttttttg
     1621 accacgtcca gaaaatcaaa cagtcggtcc gcggtttctt agccgatgta aacgagcgtc
     1681 cccttgaggt gattgaccga ctgtgcgtgc ggatgtaaag tatttaattc aacctatata
     1741 gttaattgta gcctatc
//
LOCUS       DACDLI010000157         1684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDLI010000157 DACDLI010000000
VERSION     DACDLI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.165"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..861)
                     /locus_tag="IV353_002749"
     CDS             complement(<1..861)
                     /locus_tag="IV353_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9010906.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYR"
     gene            1024..1281
                     /locus_tag="IV353_002750"
     CDS             1024..1281
                     /locus_tag="IV353_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010907.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(1513..>1684)
                     /locus_tag="IV353_002751"
     CDS             complement(1513..>1684)
                     /locus_tag="IV353_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9010908.1"
                     /translation="VQTSTELLMKIEKEKLLYITKTSPYNKTDEENFHLIVTTLQTEL
                     NDIISDFNQVNN"
BASE COUNT          614 a          258 c          226 g          586 t
ORIGIN      
        1 tcgataataa tagattcctt tgtcaacatt gcaacaagaa gaataaattg tatattcata
       61 ttttccatca ccaacatcac acaaaccttt ttgttgttct actgaaccta agatatggaa
      121 aaattgacta atactttctg actctgaatc tccagataca gaattcagct tcgtaaaagt
      181 cgctttaaca aaacgagata ctgaggataa atctccaggc aagcctatcc ctcccatacc
      241 gcggctatag gcattcaaac ttatttgatt tgaaaaatta tttttaggag tttcactcga
      301 taagacacga taattgttta aattaaataa ttgatagtca aatgaaggat tattggtaag
      361 aacgcccaca gggttatcat atatatgaag tccatctttc atactttcaa tgacaattga
      421 tttttcttta tcagctaata gccaatgtaa aggggataaa ggaagttcgt cactataatt
      481 tatatttgct aaattgatat tttttaacaa ttttttagct tctcctactg ttgagcattg
      541 tcctaaaatc caaggaataa attcaaaagg agatacattg tctttccctt cttgtatttc
      601 tttataatca gcatacccag aaaaatttag tccagccata ctcaatcctt tttcatttgt
      661 cgcatcgtaa taaagagggt agtcagctat accagcggca ataccaatca ttgcataatg
      721 agtatccaaa tcatttacct ttcgaaaatt caacttataa tttcttggag taatagtgac
      781 tacttcattg taagatattt cataatcaaa attccttcca aaataatgat cacttgttac
      841 ataagtaata gacgtacaca tagtttattc ctcctaaata tttttctgtc cacgttcata
      901 ttactccaaa gttttcctca ttccaaaaga aaagcacaga atacacaaag aagcgttttt
      961 atttgtataa actatatcaa agttctatac ttaaattatc aaataaaaag gacgtgaaga
     1021 taaatgtcaa atttagaaaa caaagaagaa aaagttgtaa acaaaattgt ttctgttgtc
     1081 aataaactgg ataaagagtt agacgaactt gatacgttat ctgaaaatcc agaaaaaaaa
     1141 cataacctaa aaaaatggtt agttgaacga aaggctattc atgaaattaa aaaagttctt
     1201 catgaagctg ataaatatga aaaatatgat gaaaaagaac ttgataaaga atttaaagaa
     1261 atcaatgact tgttactata attgatttat acgactcatt tttgagtcgt cagactgcaa
     1321 acaacactct aaatttagga gtttgtctac agtctgaata aattttaaat ttattgtcca
     1381 taatgttatt atgaatttat agcattaaga aaagagataa gactttacat ttttttagga
     1441 atgtaaagtc ttatctcttt tcttaattgt acaattagta ttatctgcac tactttctaa
     1501 ttaaataaat ttttagttat taacttgatt aaaatcagag ataatatcat ttaattcagt
     1561 ttgaagggtt gtaacgatta aatggaaatt ttcttcatct gttttattat atggggatgt
     1621 tttcgttata tataacaact tttctttttc aattttcatt aataactctg tcgaagtctg
     1681 tact
//
LOCUS       DACDLI010000158         1675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDLI010000158 DACDLI010000000
VERSION     DACDLI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.166"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(112..489)
                     /locus_tag="IV353_002752"
     CDS             complement(112..489)
                     /locus_tag="IV353_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010909.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(789..1670)
                     /gene="galU"
                     /locus_tag="IV353_002753"
     CDS             complement(789..1670)
                     /gene="galU"
                     /locus_tag="IV353_002753"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9010910.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          467 a          371 c          246 g          591 t
ORIGIN      
        1 aaaagattct tttatataag tgaaaaaagc ttttttccac caattaaata aatctctcat
       61 ctcaatagtt tatccattat tttatctttt tttgcgtata cctactaaat attaccgtct
      121 tcttctcgtt cttctacttt gttcgagaga atctgtacta gaaaaagtag ttgtatgtct
      181 accttttctg cctatccgag actttctttt catatatggg atataaatga ttagatacag
      241 tacagcaaaa aataaagcaa atacgcaagc ttcccaacta cctaacgcca attcaaccat
      301 caaggtagca accccaacgg atgctgcata ccagataaaa atcagaaacg tttctttttt
      361 tctgaccatg cgaaacatga taaatagact aataagcaat agtggcaaca caaatatcca
      421 attgcttcct tccatcagcc acttgagtat atcaagcagc gactgtgcaa cagcgactaa
      481 ttttcccata gtgaacacac accttttttt ataattaaaa aattcgcctt tttattctac
      541 tatgaatctt ctggcttggc tttcatttct cttatgtaat cattttcttt tatacaactt
      601 aaaaaaaaat taaaccatta gcataatatt ttctgaatct attcacgaaa gtctttttct
      661 atctttcgtt ttccacgact caactgatct agagtcaaaa aactaaaacg acagttgcgc
      721 aaatgacgca caagctgccg ctttaataac aaatagcctt tcattctcag caggccactt
      781 atttccagtt aactttctgt tcttagcttt ttactcaact ggataatata tgcacttaat
      841 gaagtcccaa tttccgaatg ttgaagtcca tattcaatat ttgtcttcat atatcctaac
      901 ttatcgccta cgtcgtatct ttttccttta aactctctcg catagaccgc ctctgtctgg
      961 ttaagagtct gaagtgcatc cgttagctgt atctcgtttc ctgctcctgg ttcttgagtt
     1021 tctaatatct caaatatttt aggagtcagt agataacgtc cgataatggc taaattactt
     1081 ggtgcttttc ccatttcagg tttttctaca aaccctttta ctctgcaacg acccttttcg
     1141 ctatctgttt taggatcaat aattccgtat ttagatgttt cattttctgg tacacgcatg
     1201 acggcaacag cggaagcttg tgtttcttca taatccatca tcaattgttg agtcaatggg
     1261 atatcatctt tcatcaaatc atcccccagc atgacgacaa acggttcatt tcctataaat
     1321 gcttttgctt gaagcacggc atcccccagt cctcttggat gagattgacg gatgaagtga
     1381 agattgactt gagtcgtttc ttcaactaaa gcgagtaatt ctgttttttc cttttcccgc
     1441 aaattgtttt ccaattcgat atttgcatca aagtgatctt caatgggtct tttttctttt
     1501 cccgttacga tcaagatgtc ttcgattccc gaatccaatg cttcttccac aataaactgg
     1561 atcgttggtt tatccacgat tggaagcatt tcttttgcca tggcttttgt tgccggcaaa
     1621 aaacgtgttc ctagcccagc ggcaggaatg actgcttttt tgaccttcac ttgct
//
LOCUS       DACDLI010000159         1578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDLI010000159 DACDLI010000000
VERSION     DACDLI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.167"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..446
                     /locus_tag="IV353_002754"
     CDS             <1..446
                     /locus_tag="IV353_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010911.1"
                     /translation="QPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGE
                     KNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTA
                     LSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            433..>1578
                     /locus_tag="IV353_002755"
     CDS             433..>1578
                     /locus_tag="IV353_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9010912.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPR"
BASE COUNT          510 a          208 c          252 g          608 t
ORIGIN      
        1 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
       61 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
      121 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
      181 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
      241 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
      301 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
      361 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
      421 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
      481 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
      541 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
      601 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
      661 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
      721 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
      781 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
      841 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
      901 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
      961 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     1021 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     1081 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     1141 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     1201 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     1261 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     1321 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     1381 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     1441 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     1501 aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac
     1561 atgattttca tacctagg
//
LOCUS       DACDLI010000160         1527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDLI010000160 DACDLI010000000
VERSION     DACDLI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.168"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            143..676
                     /locus_tag="IV353_002756"
     CDS             143..676
                     /locus_tag="IV353_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP9010913.1"
                     /translation="MESGKKSPVYNVKSVDIKKVFPNDYNPNSVATPEMKLLYESIKN
                     DGYTMPIVCVYDEVEDKYIIVDGFHRYRVMMEHRDIYDRENGMLPVSVIDKPIDQRMA
                     STIRHNRARGSHDVDLMSNIVKELHELGRSDAWIAKHLGMDSDEILRLKQITGLASLF
                     KEHEFSDAWEPDEELLE"
BASE COUNT          581 a          161 c          281 g          504 t
ORIGIN      
        1 tcggtttaac ccgtatccaa caaagaaagt tagactttct taaaagtaaa tacaaaagta
       61 tcgatgatat ataaatatat aatatattaa atatctttga ttgattatag aaatgatagt
      121 tagttacagg aagtgaatgt aaatggaatc agggaaaaaa agtcctgttt ataatgttaa
      181 atcagtagat ataaagaaag tatttccgaa tgattataat cctaattcgg tagcaactcc
      241 ggaaatgaag ttattgtatg aaagtattaa gaatgatggt tatacgatgc ctattgtatg
      301 tgtttacgat gaagttgaag ataagtatat tattgttgat ggtttccatc gttatcgagt
      361 aatgatggaa catcgagata tatatgatag agagaatggt atgcttcctg tttcggttat
      421 tgataagcca atagaccaaa ggatggctag tactatacgg cataatcgtg cacgaggaag
      481 ccatgatgta gatttaatga gtaatatagt taaagaattg catgagcttg gacgttcaga
      541 tgcgtggata gctaagcatc ttggaatgga tagtgatgag atattgcgtt taaagcaaat
      601 caccggatta gcttcattat ttaaggagca tgagtttagc gatgcttggg aaccagatga
      661 agaattactt gagtaacctc tattgcattc agtagaatga ttcattgaca atagttcttt
      721 ttagtaataa tcattgtcaa tgaatccata caacgtcaaa ttacttatac aattatagac
      781 aaatttatat tgtgagtgtt acaaaattga atacaaattt gttttctgct cataataatc
      841 gtacctactg aacaatagaa atgtttctgt gagttttaat gaaaagtgaa ggtagtatca
      901 tacttttcat attttagata gcgaacttag taactgtttt aaataaagaa ttaataggaa
      961 tggattatgt ttagataaac agaaggaatg cttgtaataa aaaaattttc agtatgttaa
     1021 tgctattttc tttagtttat tgcattagat taaataaagt atttactgat gaaacagatc
     1081 aattgcttta taaagtaagt ttatctattt aaaatatatt tttatccgga ttcataaaat
     1141 ttatatgtgt taaagagtga agctccaaga aataaatcga actttcattt agatagaaac
     1201 taaactgcta tttgggagga ttagataagc cggatgaaga aatgaatgaa ctaaatcggg
     1261 agatattaag ggatatagga acaggttcag aaaatattgg caataataaa cctagaacaa
     1321 ataagtgtta acctgatgaa ttattaggtt acaagttgcc tgattctttt ttaataaaag
     1381 aattagactt taaagatcag tttaaaaatg gagaagatta gtatgtatag atatataata
     1441 gagagtatta attaaacatt ctattcgtac aaaaaaatgg ctagggaaca aaatattgaa
     1501 tctataacaa tagattaccc taaaata
//
LOCUS       DACDLI010000161         1503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDLI010000161 DACDLI010000000
VERSION     DACDLI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.169"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            254..433
                     /locus_tag="IV353_002757"
                     /pseudo
     CDS             254..433
                     /locus_tag="IV353_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            492..845
                     /locus_tag="IV353_002758"
                     /pseudo
     CDS             492..845
                     /locus_tag="IV353_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1009..>1503
                     /locus_tag="IV353_002759"
     CDS             1009..>1503
                     /locus_tag="IV353_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAP9010914.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHFFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDAT"
BASE COUNT          523 a          241 c          293 g          446 t
ORIGIN      
        1 caaagcccga aaacctaaga gaaagaagga gatttatatg aacaaaatta ttcccttatt
       61 attagtccta tttgttggag aaatacttat gcgacttagt gcagctgtac atttaaacct
      121 cgcagtattt ttggtaatac taatgttttg tttgatcttt tggcgatttg tgttagccct
      181 tgtgagtcta atattcaagt tgattgcagc tatatttttc atagggttat ttcgatgaaa
      241 ggagagagta ccaatgccaa ttgcaatagg aaataaacgg ttacccgtga cgttggatga
      301 aaagagacaa aaagaattgc agtaaccaaa gcagaagtac ggcaaaagtg aatccaggat
      361 tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atgtaccagc
      421 actaaaaaag tgacgtcact ttttaacttg atccgtataa gtgatatcac ttttagcaag
      481 gagggaaata aatgttaaaa actaaaaaga attcgtacaa attaaagctt agtatactat
      541 ctattatttg tcttgtagat atgataatta gtggtcattt gtatagagtg gcatatacgc
      601 agtttattga gtacaaggtt cactatttag gattagctgg gaatcgatac gttgaatatg
      661 tccattattc attaaaatgt tcttcgtata ttcttctttg gcttattttt tcattgtttg
      721 aattgctaaa tttaatagca atatcttcat atcacttaga taagctaaac accaaatcta
      781 gccgttataa cgaagtgttc aaggaaaata tgaagttaga aagtgactta aacaagctaa
      841 aaaaaataaa aagaagaaaa cataagttaa ctaaaattat tttattaaga atgatggaat
      901 ataaggataa ggctgagagt gtaaaatatt ttgtgtaaat agaaaaaagg aagtcccttc
      961 tgtagaatag agttaccaca acacattcac agaaaagagg acttccatat gaacgatttt
     1021 actacagaaa ttctaaagac tctagcgaac aaaggcgatt tgaatgaatt attccgtgtc
     1081 catttggaaa aagctgtcaa tacgcttctc aaaacggagt taacggcttt cctcgattac
     1141 gaaaagtacg atcgcattgg ttttaacacg ggtaattctc gtaacggctc ctatgaccgt
     1201 acggtcaaga ccgagtacgg ggaacttcat ctccagattc cgcgcgaccg caacggcgag
     1261 ttcaagcaac agactgttcc tgcttataga cggacgaatg ataccttaga ggagaccgtc
     1321 attcacttct tccgaaaagg tattaccatg tcggaaatcg cagacttgat tgagaaaatg
     1381 tatgggcatc actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa
     1441 gtaaatgcat ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct
     1501 act
//
LOCUS       DACDLI010000162         1461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDLI010000162 DACDLI010000000
VERSION     DACDLI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.170"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1461)
                     /locus_tag="IV353_002760"
     CDS             complement(<1..>1461)
                     /locus_tag="IV353_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9010915.1"
                     /translation="EIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYPW
                     LTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTS
                     SFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSV
                     AMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNR
                     PYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGILSKITYI
                     MGIISILLCTKIFPWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYT
                     RRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKK
                     DQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKN
                     ATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLF"
BASE COUNT          564 a          201 c          198 g          498 t
ORIGIN      
        1 aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt tatttattcc
       61 actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag cattttttga
      121 attaacagta accgttaatc ctttataccg gaatacaggt aaaactattg ttttgccttt
      181 tgaagattta aacgtaaatg taatttcatc tttatttgga gaaaagctac tataaataac
      241 ttgaccatct tctgtaactt gatggttttt caaaagcaat tgatcttttt tatagcctat
      301 aggtacataa tcaaaattct caaattcttc aagtttattt tccatttgtt cattagaata
      361 agttccttga aaattcattt ctttcaccat gttaactact gatgaataat ttaaaattga
      421 aatagctaat attaataata gtataaaatt tcttctcctt gtataagaaa atttttccaa
      481 ctgtggagaa ataaatagag atactgaaaa agatatgaac aatgttgaaa atatcataaa
      541 acgccaagga gcttgaataa tatttaaagg agtattttgt aaggcaaacc atggaaatat
      601 ttttgtacaa agtagaatag atattatacc cattatataa gtgattttag ataagattcc
      661 taatttttta aagttaaatg ttagtataat taaacaaata agcaaagtta agccaatacc
      721 aaatgatagt aattctgaat ctatacttcc aatcaaaaac tctttaccca gataaattgc
      781 tcttttttgt aatatatcca cataaggtct attcagatct tgatacaatg tctgctcaat
      841 cataggacct ataaacgcta gtgaaagaag taaagtaact atagcagctt ttattaattt
      901 aataattaag aagatttcta ttttcttttt ccgataaaat tgaataatta aagcaattat
      961 aatcattacc atagtcatag ctacagaaag aacatgagtg taaattaata aagtcatgct
     1021 aatcgccaac ataacccaag attctttttt tgagtcgtaa agctttataa acccgagtaa
     1081 aataagagga agtattgaca ttgcgagtaa ttcccccatt gcccctcgaa gaaatatttc
     1141 tacactccta aaagaagatg ttgtatatat aatagcgaaa aaggagctag taacatgttt
     1201 tttggtaatt tcatacatta cataatgaca tataaataga gtaatgagcg taacaatata
     1261 aagaaaaagg ttatagccta ttgctaaatt agtaaacttg atcaataaaa ataaaggata
     1321 tagagttaac catgggtaaa aattatttac ttgcatgcca tatgatctaa acgtttcaaa
     1381 attaataggg gagctaaaaa tagattctaa acttaacgca cgattaatgt gaaatgccgt
     1441 atcataaaag ccaaatattt c
//
LOCUS       DACDLI010000163         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDLI010000163 DACDLI010000000
VERSION     DACDLI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.171"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            348..701
                     /locus_tag="IV353_002761"
     CDS             348..701
                     /locus_tag="IV353_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010916.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
     gene            complement(1111..>1455)
                     /locus_tag="IV353_002762"
     CDS             complement(1111..>1455)
                     /locus_tag="IV353_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP9010917.1"
                     /translation="QPREPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHK
                     LFVRGYSAIQHPHKPDVTERRKKIFYDRYINGMSIYVTAQRNNTSEESVKAESNKIII
                     QFASSLELVAFK"
BASE COUNT          488 a          233 c          239 g          495 t
ORIGIN      
        1 acattaagac agagcgcaaa aattgaaatt attaatggaa tcatttctct tcacccacct
       61 ttagttatcg tgtgaataat taaaaaacaa tagacagcaa caaaataaca ttgctttgat
      121 aatttggtat aaaccactat aaatttcttt tcttgcaatt atttttaata tatgctagat
      181 tatcaaccga tatagtcact gcctgtacta gcggaaacta gtgcaggttt tttgttctat
      241 ttactcaaaa gttattacga taaattaata ttgtgaaaat aaatactaag cgtataattt
      301 tatttatcag cgagtggtcc gctgaaataa tttaaggtgg taaaaaaatg gcaaaaattg
      361 atgattaccg ttcagacatg attgaatata ttgaactctt cgctaaaaat tctaacaata
      421 atattattat tcaaactgga ggagcaacaa tttgcggaac tcctatagac tttgatgctg
      481 aagtaaaagt aaatcctctt attgatgcta tgatggattc ttttgcagaa tttcgctcaa
      541 aaaaaattga tgatatagaa aaagatgatg agcaaagttt ggtagtcaaa tctatctttc
      601 tgaaagatgt taccatcata ggcgagagaa ctactaatat cccattttta gttgtttttg
      661 ctgatcagat ttctgcaatt tctctcggga acttggagta acaatagatt catttaaatt
      721 atctatattg attaaattgc taatctctcg gctattacaa atagctgaga ggatttcttt
      781 tatttctcct gaagtacctt caatcgataa tttcatattc ttaccctcca atacataaat
      841 taatagacag caacggatga tagataataa gaacaattta gaaggagttg aaattcacat
      901 ccttattctt aatatttccg ttgctgccta tcgaagctta attaaacgat gagggagatt
      961 tcctccctta cattttattt tgtcgatcct gtttcctaat ctttcgacac tatcataata
     1021 caacgttgaa taggtaagtg attggtataa aaaaggtata aaatggaaac caaattggta
     1081 ataaaagggt ataaaaagtg taaaaactgg ctacttgaaa gcaaccagtt ctaaggaaga
     1141 agcaaattga atgatgattt tgtttgattc tgcttttaca gattcctcgc tcgtattatt
     1201 tctttgggca gttacataaa tcgacatgcc attgatataa cgatcataga atatcttctt
     1261 gcgcctttca gtcacatcag gcttatgcgg atgctgtatc gctgaatagc ctcggacaaa
     1321 caatttatgc agatactcaa attcttcctg tgcttcttct ttatcgatca gcattctttc
     1381 tgcttcaaat atatgatcag ctgtagaagg tggaaccaag gaataagatg ctgtcacttt
     1441 tggttctcga ggttg
//
LOCUS       DACDLI010000164         1320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDLI010000164 DACDLI010000000
VERSION     DACDLI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.172"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(241..855)
                     /locus_tag="IV353_002763"
     CDS             complement(241..855)
                     /locus_tag="IV353_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9010918.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          439 a          254 c          220 g          407 t
ORIGIN      
        1 ttttattttt ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt
       61 ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat
      121 aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca
      181 aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa
      241 ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag
      301 caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt
      361 caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa
      421 tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat
      481 gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct
      541 tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat
      601 tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat
      661 acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg
      721 gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc
      781 ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa
      841 ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg
      901 cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc
      961 tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa
     1021 aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg
     1081 tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat
     1141 gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt
     1201 attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa
     1261 aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc
//
LOCUS       DACDLI010000165         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDLI010000165 DACDLI010000000
VERSION     DACDLI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.173"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            254..433
                     /locus_tag="IV353_002764"
                     /pseudo
     CDS             254..433
                     /locus_tag="IV353_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            495..679
                     /locus_tag="IV353_002765"
                     /pseudo
     CDS             495..679
                     /locus_tag="IV353_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            713..1144
                     /locus_tag="IV353_002766"
     CDS             713..1144
                     /locus_tag="IV353_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP9010919.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT          437 a          199 c          266 g          400 t
ORIGIN      
        1 caaagcccga aaacctaaga gaaagaagga gatttatatg aacaaaatta ttcccttatt
       61 attagtccta tttgttggag aaatacttat gcgacttagt gcagctgtac atttaaacct
      121 cgcagtattt ttggtaatac taatgttttg tttgatcttt tggcgatttg tattagccct
      181 cgtaagtcta ctattcaaga tgattgcagc catatttttc ataggattat ttcgatgaaa
      241 ggagagagta ccaatgccaa ttgcaatagg aaataaacgg ttacccgtga cgttggatga
      301 aaagagacaa aaagaattgc agtaaccaaa gcagaagtac ggcaaaagtg aatccaggat
      361 tatgtgtatt gcgttagatt tattgattgc gcaagaaaaa gcaggatttg acataccagc
      421 actcaaaaag tgatcgcact ttgcgcacgt gttaatacgt gttataatta ttttgtaagg
      481 aggtatagtg cacaatgcca cttacgggaa aagaaatgct taagttattg aaaaaaatgg
      541 ctgggttgag cgaaggcaag agggatttca tcatcatctt tataaggatg gtgttagaat
      601 cactgttcct gttcatgcca atcaagatct tggccgagga cttgagcgaa aaattttaaa
      661 ggatgcggga ttgaaataat ctcgcattcg attaaagaaa ggattgactg aaatgttaag
      721 tgagccttta gcaaaaacct atcctgctat ttttagccca gaagaaggtg gtgggtactt
      781 tattgaattt cctgatgttc aaggtgctta cacaggaatt aacgaagacg atatatctta
      841 tggaattgct atggcacaag aggtgttagg tatggtttta gcagattata ttgagcatga
      901 agatctactt ccagaaccaa caccaatcaa taagatttct gttgaagatg attcttttac
      961 tactttaatt cgtgtagatg ttgccaaata tttgaaagac acagaacttg tcaaaaaaac
     1021 cttaacaatt cctcaatggg cggataagct aggcaaaagg gctggcataa atttttcagt
     1081 ccttctaaca gaatcaattg cagataaagc agataacatt cttcgttctg gaagaaataa
     1141 ttagaactca aaagggtcag aaacttaaaa atctttggct tcttttttta ctacaaagat
     1201 ggcttcttta aattatttgc ctgattgatt taaaaaaatt gcgcaaagac tacgagctaa
     1261 ttaagttgct cgtggtcttt ttttatgaaa actttttaga tc
//
LOCUS       DACDLI010000166         1287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDLI010000166 DACDLI010000000
VERSION     DACDLI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.174"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..1237
                     /locus_tag="IV353_002767"
     CDS             join(75..351,351..1237)
                     /locus_tag="IV353_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9010920.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          465 a          227 c          257 g          338 t
ORIGIN      
        1 ggataggcta caattaacta gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac
       61 caggaggaat ttttatgtct aagagaacac gaagaacttt ttcacaagaa ttcaagcaac
      121 aaatcgtcaa tctttactta gctggaaagc cacgtgtaga aatcattcga gaatatgaac
      181 taacggcttc agcatttgac aaatgggtaa agcaatctaa aacgagtggt tcattcaaag
      241 aaaaagataa tcttacgcct gaacaaaaag aattgttaga actacgtaaa agaaaccagc
      301 aattagaaat ggaaaatgat attttaaagc aagcagcgct gatattcgga cgaagagaca
      361 agtaatcgat gcgaataagc atctttaccc tatatcagcg atgtgcagaa tattaggtct
      421 atcacgtcag tcctattatt atcaatcaaa accaaagaaa gacgaatcag aacttgaaga
      481 agtagtcgct gaagaattta tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa
      541 agccttatca aaacgaggca ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa
      601 tagaggatta aaatcgagct atactgttgc ttattttaaa gtacatcatt ctacttgcaa
      661 tgaagccaaa acgacaaacg tattgaatcg taaattctta agagacaacc cattagaagc
      721 gatcgtaaca gacttgactt atgtacgagt cgggaaaaaa tggaattatg tctgtttcat
      781 tttggatctg ttcaatcgag aaattctcgg ctattcttgt ggagaacata aagatgccgt
      841 tctagtaaaa aaagcattta gccgtatcaa acaacctctg acagaggttg agatttttca
      901 tactgatcgt ggaaaagagt ttgataacca agctattgat gaattattaa caacttttga
      961 catcaatcga tcattgagtc ataaaggctg tccttttgat aatgccgtag ctgaatcaac
     1021 ttataagtcg ttgaaagtag aatttgtcta tcaatacaca tttgaaacct tacaacaatt
     1081 ggatttggag ttatttgact atgtcaattg gtggaaccac cttcggttgc acggtacact
     1141 tggctacgag acaccggttg gttaccgtaa ccagagattg gcgcagcgaa tccttgataa
     1201 tgagctcgga tgtgctaacg ctagcgaggc agtctaactt taactgttag ctcctgccga
     1261 agatcgtcac atccgaggag gctcatt
//
LOCUS       DACDLI010000167         1266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDLI010000167 DACDLI010000000
VERSION     DACDLI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.175"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..728)
                     /locus_tag="IV353_002768"
     CDS             complement(<1..728)
                     /locus_tag="IV353_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9010921.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYP"
BASE COUNT          379 a          203 c          216 g          468 t
ORIGIN      
        1 ttaggataaa tttgatggat agtatctttc atgcctttta agccgtccgt aataaaaagc
       61 aagacttctt gaactcctct ggagttaata tcctgtagca gctcattcca aacgtatgtt
      121 gattcagttg gagcaatcgc ataactcagt acttctttag tgccgtcttc tcgtatacca
      181 atggcaatat aaatcgcttc tttggatacg gtttgacgtt ttagtggaat gtaagtagcg
      241 tccataaaaa tagcgacata cttatcattt aaggctctgg atttaaaggc atttacttct
      301 tcagtcagaa ctttagtcat gttggacatg gtttgtggag tatagtgatg accgtacatt
      361 ttttcgatca aatcagcaat ttcagacatc gtaacacctt tttcgaataa atggataata
      421 gtggtttcca atgtatcgtt tgttcttttg taggctggta aagtttgttg tttaaactca
      481 ccattacgat ctctaggtat ttccaatgtt aattcaccat attcggtttt gattgatcga
      541 aagtaagaac cgtttctcga attacctgaa ttaaaaccag tgcgatcata tttttcgtaa
      601 tctaaaaaag ccgttaattc agtccgtagg agtgtgttta tcgctttttc taagtgcgaa
      661 cggaataatt catttaaatc gcctttagtg actagagttt gcacaatttc tgtagtaaaa
      721 tcattcatag ggaagtcctc ttttctgtga attggttgtc gttaacttta ttctacagaa
      781 gcgacttcct tttttgtatg gattttttca tttacacaaa atattttaca ctctctattt
      841 tagtcttgtc ctttaataca aagattttat ttttaaataa aaaatactct ttttttacga
      901 ttattgaaaa aaaaagataa aacttgatta attgaagtat tgcttaaatt tggaagctat
      961 gagggaagaa taaaagagtt aggacgggat tatctactat ttttctttgt tttaaatatt
     1021 tgttatcttt gaacataaaa gagaaatgtg tcgactgcag tgttgtgtcc ctcatcacga
     1081 ataaacggtg ttcaattagc tgatcttgta ttcacttaaa aaatggtaaa gtacgagttg
     1141 ctattcccca ttttcaattt tctaacgctc aggacaaaat actgttttca caacctcatt
     1201 ggaataaaag catatatatt gtttcaagaa cgaaatcttg ttatcttttt aaagggcatc
     1261 tgtcca
//
LOCUS       DACDLI010000168         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDLI010000168 DACDLI010000000
VERSION     DACDLI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.176"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IV353_002769"
     CDS             200..742
                     /locus_tag="IV353_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010922.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IV353_002770"
     CDS             832..>1246
                     /locus_tag="IV353_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9010923.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          352 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaaaacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDLI010000169         1214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDLI010000169 DACDLI010000000
VERSION     DACDLI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.177"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            70..1026
                     /locus_tag="IV353_002771"
     CDS             70..1026
                     /locus_tag="IV353_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010924.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="IV353_002772"
     CDS             complement(1080..>1214)
                     /locus_tag="IV353_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9010925.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DACDLI010000170         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDLI010000170 DACDLI010000000
VERSION     DACDLI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.178"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..273
                     /locus_tag="IV353_002773"
     CDS             <1..273
                     /locus_tag="IV353_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9010926.1"
                     /translation="VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQC
                     GIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            278..>1195
                     /locus_tag="IV353_002774"
     CDS             278..>1195
                     /locus_tag="IV353_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9010927.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGM"
BASE COUNT          385 a          177 c          249 g          384 t
ORIGIN      
        1 gttactatag ttaataaatc aagatacaca tctaggcaac agacacctga agtttttgaa
       61 atgaatgcct caatgtattc ttttaaacct gaatttctaa aaaaaagtaa ttatattttt
      121 gatgggcaat gtgggataat aaaaatgaga gattatctaa ttttagatat tgatagtgaa
      181 gaagatttta aatggttaga atttttgttt cctaaatttt tagaagattc ttcggagctt
      241 caagatatct ataattattt atatcggagg taaaaggatg agctgttatc ttatcaccgg
      301 tggggctggc ttcattggtt ctaccttagc caatcattta ggagaagaac atgtggtaat
      361 cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt gatatgacga aaaatatcac
      421 ttttattgag ggagatgtcg ctgagaaatc cttgatggaa gagatcatga aagctaataa
      481 atttgattat atctttcatt tagcagcagt tgctagtgtg gcagattcag ttgcccgacc
      541 agtagaaaca catcgtgtga attttgaaag tgtattaatg attttagaat tgatccgcaa
      601 gtatcaacct gatttaaaac ggatcgtgtt tagttcttca gcggctgttt atggggatga
      661 accgacttta ccaaaaaaag aagagtccgt gattcgccca ttgacgcctt atgcggttga
      721 taaattcgct gcagaacaat atgtgctaga ttattgtcat ttatatgatg tacccggtag
      781 cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat ccgaattctc catacagtgg
      841 tgtgatttct attctcgtag atcgctataa gaaacaaatc gtaggagaag caacttcatt
      901 tacattattt ggtgatggaa gccagtctag agactttgtt tatatagatg atgtaatcca
      961 agcattactt ttagttgcga atgaggaagc tgcactgggg aaacaattca atgttggaac
     1021 gggtaaagca accacacttt tggaattgat tcatgcaatc aatgaaattc tcggtgtaga
     1081 attgacactt gaatacaaag aagagcgttc gggtgatatc catgactctc tagcagatat
     1141 tactaagatc cagtcaattg gctatcaacc gaaatatggt attttaagtg gaatg
//
LOCUS       DACDLI010000171         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDLI010000171 DACDLI010000000
VERSION     DACDLI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.179"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV353_002775"
     CDS             <1..1076
                     /locus_tag="IV353_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9010928.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACDLI010000172         1154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDLI010000172 DACDLI010000000
VERSION     DACDLI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.180"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(64..207)
                     /locus_tag="IV353_002776"
                     /pseudo
     CDS             complement(64..207)
                     /locus_tag="IV353_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            289..1065
                     /locus_tag="IV353_002777"
     CDS             289..1065
                     /locus_tag="IV353_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9010929.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT          431 a          152 c          184 g          387 t
ORIGIN      
        1 ataaaaaaac aaacaaagta aaataatcta ctttgtttgt ttttatttta attaatattg
       61 aatttataaa attgtcttat cgcttggttc cacaaccaag tagcgataag gctcatcccc
      121 ttcagaatga gtggtaacat aaggattttt acttaaaaca gaatgaattt gttttctttc
      181 aaaagcgggc attggttagt caaagactag ttcatttata tttatatctt aatcttttat
      241 tttaataaat tgtgatataa tgaagtataa ctttttaggg agtgtactat gaattttcaa
      301 gataatagac cattatatct acaagtagaa gcttctatac gtaacgatat tcgtgaaaaa
      361 aaatatcttc caggagagca attacctaca gaagaagaat tatgtaaaat ttatggagta
      421 agtaagatta ccattcgaaa agcttttaaa cttttaacag aaagtggaac agttgaacga
      481 ttaaggggga aagggacctt tgtaactcaa aaaaaagaaa gtttaaaatt agctggagtt
      541 catggattta gcgattctct ttctacccga ggacataaaa ttagatatac tgttttacat
      601 tctaaaatta taaaggctga tgagttttta gctaataaat tacaaatcaa tgtaactgat
      661 agagtttaca atattaaacg tttaatgtgg gaagacggtg ccccgatggg aattgataat
      721 tttttcgcat cagaagaaaa atttccaact ttatttgaaa aaggtagcca atatacttca
      781 ttatataaac ttctagaaga tgactatgat gtcaaagcta cgagctctat tatggaaata
      841 aacggtttat ctgcaacacc tgagctttcc gacttattgc aatgtatagt aggagatccg
      901 ctatttattt tagaaaaggt tggcaaagat aaaaataatc aaccatttca ttattctcaa
      961 tccacagttc gttgtgatag aattagttat gttattgaag tcacagaaaa tatcacttta
     1021 gatacggtgc ataaaaatga ttcagatcct aaagtaatag attgatatta aaagaaatca
     1081 tacccaaagc aatcaatttt gattagattt gggcatgatt tttattttta ctaatattct
     1141 ctaaatacat caaa
//
LOCUS       DACDLI010000173         1123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDLI010000173 DACDLI010000000
VERSION     DACDLI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.181"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..>1123
                     /locus_tag="IV353_002778"
     CDS             14..>1123
                     /locus_tag="IV353_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9010930.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMEN"
BASE COUNT          404 a          194 c          219 g          306 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aat
//
LOCUS       DACDLI010000174         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDLI010000174 DACDLI010000000
VERSION     DACDLI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.182"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            440..1073
                     /locus_tag="IV353_002779"
                     /pseudo
     CDS             440..1073
                     /locus_tag="IV353_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          372 a          185 c          190 g          326 t
ORIGIN      
        1 agattagtca ctatataaaa tgtgattaat tttattatta ataaaatatt ttttatatta
       61 caaaagtagt gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag
      121 aaagaaatga acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga
      181 aaaacaatct taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt
      241 tagcaagcta gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa
      301 atcattatgg atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa
      361 ttcgcaaaat ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc
      421 ccattgttat tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa
      481 gccgtcaaaa aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc
      541 aattgtggat aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat
      601 tttgcctaca cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga
      661 agcggtggaa tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt
      721 cttaccaaat gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc
      781 gttcaatgat tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga
      841 agccgagaaa gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca
      901 ctatgattac aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc
      961 aatgatgatt gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt
     1021 ccatgagctg accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DACDLI010000175         1070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDLI010000175 DACDLI010000000
VERSION     DACDLI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.183"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..>1070
                     /locus_tag="IV353_002780"
     CDS             25..>1070
                     /locus_tag="IV353_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9010931.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKY
                     MSGKSLEALELEQEESIRFQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKS
                     ELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLD
                     ALLEISDSDPLLQY"
BASE COUNT          386 a          169 c          223 g          292 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
      541 caagaggaaa gcataagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      601 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      661 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      721 agacaacgtc ttgcatatgt acgcctttat ggcggaatcc tgcatttgcg ggattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
      961 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1021 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta
//
LOCUS       DACDLI010000176         1065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDLI010000176 DACDLI010000000
VERSION     DACDLI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.184"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            241..1002
                     /locus_tag="IV353_002781"
     CDS             241..1002
                     /locus_tag="IV353_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAP9010932.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
BASE COUNT          302 a          168 c          202 g          393 t
ORIGIN      
        1 tgctgtagtt tcaagaaaat ttacaggaaa tttgataact gatattattt actttttgac
       61 caccatatac atgactttta tttttgtttc ttttttagaa tcaaatgtga ttaaatagaa
      121 aattttacaa taatatgcac atataaataa taagatgata taatcaacaa agaaaatgtg
      181 atttttttca ctatatttta tcacatgaaa attcattaag aaagtaggaa tcgaagtatt
      241 atgcacacac cagaagaaat tatggaactc agtattcatc ttggaacaaa aaaaatacat
      301 aaacctatct tagctaaact gattttaggt tttatcggag gagccatgat ttctctaggt
      361 tatttggcat acgtacgagt ctctgcttct atcccagaat ctatggctag cttgactagt
      421 ttagttggtg cctcggtttt tcctatcggc ctgctggtca tccttcttgg cggtggtgaa
      481 ttgatcaccg gaaatatgat ggctgtggcg attgccttta tggataaaaa agtcacattc
      541 tcacagctgg ttaagaattg gtcagtgatt acttttgcaa atattattgg tgcaattttc
      601 gttgcctatt tctttggaca ctttgttggt ttaactcatt caggtgtcta tctcgaccaa
      661 gtgattcata ttgctgatca taaaatcgct gtttcttggt ggcaagcatt aggctccgga
      721 ataggttgta attggtttgt cggtttagcg ttatggttat catatggagc aaaagatgcg
      781 gccggcaaag tactaacgat ttggtttcca gtcatgattt ttgtggcaat cggtttccag
      841 catagtgtgg ccaactgttt tgtgatacct gctgccatat ttgaaggtca agcgacttgg
      901 ttagaatttc tctcgaattt tatccccgtt tatattggaa atatcatcgg cggagctatt
      961 tttgtttcag gtttttatta cgttagttat aagcatcatt aagattgaag gtaatgttca
     1021 ttcgagcatt accttcaatc tctttatata ctatttatat tttaa
//
LOCUS       DACDLI010000177         1055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDLI010000177 DACDLI010000000
VERSION     DACDLI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.185"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1035)
                     /locus_tag="IV353_002782"
     CDS             complement(<1..1035)
                     /locus_tag="IV353_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9010933.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEE"
BASE COUNT          393 a          166 c          126 g          370 t
ORIGIN      
        1 ttcttcgata tacgaacgat taaaaaaaag atctggatta ccataaaata gataacttct
       61 ttcatgaaat gcaataaaat tgaatatgag ttctggatcc tttatatcta aagaaaaacg
      121 gaacatgctt tgtaaaactt tctccaaatt ctcaactgct cgataagaac aagtattttg
      181 tcgctgatgt gcatatgcat gactattttc atatattgca tttccatcaa ttactccaaa
      241 agaataaatt attcgataaa ggaaacacag ttcatcgctg ttaaaagaaa gattgatttg
      301 ttttgaccac ttcttcaaaa gattaaaatg cggatcatct gagataactg atttatattc
      361 attatctact cgtacttttc taaatgaact tctttctata ttaatggcta accaaaaaga
      421 aaatacacgt ttttgaaaaa tagttaacgt aatatcataa atcccctcaa aagcaactat
      481 atacttctct atttcctttt gttcgatatg aaatggccac ttatcactac tttcacgtgt
      541 agaattccaa tagaaataat aaaaaaagct acgaatgttt gcctctgatg cattcaattc
      601 taactgaaat ggatgaaatg ttatatgata acgcctaaaa tatggaatta agagcttcgc
      661 atatctgaaa acagtagata cactagtaaa ttgctcttta gcaaaatctt ttagaacact
      721 tctaggatat ttatacataa aatcaatcaa atgataatta taagaagctt tataaaaatg
      781 aagataaata ttataaatag gaaaatcttt ctcttttttt atgaaaaatc gtttattatc
      841 ttcatttttc agaaggatat taccattata ttgcttaaat aaatcctcta gttcttctat
      901 atagtgtaag actgacttct ccgtaacatt aagtaaattt ctaagttctt ccattgcaca
      961 ccaattgtct ttttctaaca aataatctaa taaaactact tttttgaaag atgttttagt
     1021 taaaaaagtt tgcatcattt cacctcaata agtta
//
LOCUS       DACDLI010000178         1024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDLI010000178 DACDLI010000000
VERSION     DACDLI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.186"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..940)
                     /locus_tag="IV353_002783"
     CDS             complement(53..940)
                     /locus_tag="IV353_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9010934.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          303 a          210 c          183 g          328 t
ORIGIN      
        1 aaaaagacaa agatatacca tatatggtaa tttctttgcc tttttatcta aatcaaacgt
       61 ttagttcttg accaactaat aaagaataac tggacataga catgccattt ttcgccatta
      121 gatcatcaac agacatgcca aatttttgtg caatatccca tagcgtatca cctgacacga
      181 ctgtatatgt ctgaccatta tttgtattta catctgttgt tgaagatgat gacgttgaag
      241 tcgtcgtagt agtcgttgtt gttgtacttg tggttgttgt agccgaacct gttgatggcg
      301 tatcaaaacg ggttaaatta taagtattaa tcaatgagtt caatttggcc ccataatttg
      361 gatcggtagc ataacgacct gtcaagtacg ctgttgcatc ataaaaactt gttgtattgc
      421 ttttccacac gccagcataa tgataatcgc ccgtggaaaa actggttgaa cgaagaacat
      481 tcgcatggtc ttggaaggat tcccaataag aagaatattt tcggaatggc tcattcatcg
      541 ttacccattg tccgtttaca tattctttgg ttgccatata aacgacctgt ccgttatagc
      601 ttcctttcac accgaataga ttataatttg gcgcactagc taatccggaa gtgccatatg
      661 cactttccaa caacgcttga gcgatcatga ctgaagcata caagtcatta cttgcagcaa
      721 cttctgacgc agaccagccg atttgatcga taaacgcttg aggatcagcg actgtttgag
      781 atccttgttc atctgcatga acatgttgcg gtaccgctgt aaccatcatc ggcccaacta
      841 aaagactcgt tccaattaaa ggagcaatcg cttttttatt gatcaccatt tttcttttag
      901 gtctatggtg ccgggcactg cgggtcttca gctcttgcat tcaaattcct cctcaaaata
      961 aaaaaactta ataaaaagtt ctcttcttaa aacaaactta attaattaaa ttatactttt
     1021 aatt
//
LOCUS       DACDLI010000179          990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDLI010000179 DACDLI010000000
VERSION     DACDLI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.187"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            62..>990
                     /locus_tag="IV353_002784"
     CDS             62..>990
                     /locus_tag="IV353_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9010935.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAP"
BASE COUNT          338 a          181 c          192 g          279 t
ORIGIN      
        1 tatcgttctc tagtatgatt aaatcacgac aaaataaaca actgagagga cgatatgcta
       61 tatgaatgat tctatcaaaa aaatgctgcg attaatagag aaagatttga tgattacaga
      121 ggtctcttac gagacctttc agaagaaaaa gacgttgatc gtcgatgctg ttttctcacc
      181 tgctccccat acctgtagaa attgtggttc tactgtggta gatggaaacg ggaaagtaat
      241 tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt gaacaataca atcacatgcc
      301 tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa aactgtcgaa cccattggac
      361 ggctcaaagt tattttgttc aacccagaca ttcaatcgcg aatcatgtca gatataaaat
      421 tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaaatt gtcaggtatc
      481 tttgaccacc gttattcgta cgttgaaaga gtttaaaagc tacttaccaa agcaatctaa
      541 gaggattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt ccatagaaga
      601 taaaatgagc tttatttgcg cagatggcga aacaggacaa ttaattgatg tcttgcctac
      661 gcgtaaattg cctcgattaa caagctattt cttaggctgc gctaatcctg aagaagtaaa
      721 atttttagtc acagacatga acgccgccta cttccagctt accaaacgta ttctgccaaa
      781 tgcgaaagta gtgattgatc ggtttcatat tatcaaacac atgaatcaag cctttaatga
      841 gttgcgtatc cgtgaaatga atgaacttcg taaagtagga cagaaaagtc aggcagaaaa
      901 actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata
      961 caaaacatgg aaaagttttc gagcaccaaa
//
LOCUS       DACDLI010000180          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDLI010000180 DACDLI010000000
VERSION     DACDLI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.188"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IV353_002785"
     rRNA            2..>943
                     /locus_tag="IV353_002785"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACDLI010000181          904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDLI010000181 DACDLI010000000
VERSION     DACDLI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.189"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(675..>904)
                     /locus_tag="IV353_002786"
     CDS             complement(675..>904)
                     /locus_tag="IV353_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9010936.1"
                     /translation="NSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFY
                     QALKYARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          307 a          117 c          170 g          310 t
ORIGIN      
        1 gacggtatgt ggttaaaaaa gaaaaatagc taggagattt ttgagagatg actggtaaat
       61 ttaaaaatga tagaaatgga gagtatgtat gagtaataaa caatttttat ggtcttcttt
      121 tgcatttatt gttgttgata tttttttccg atattttaat aacgatatga tatttaactg
      181 gcaaagaatt ataatcgatt actgtatttt ttgtataata tattttttag tgaaaacatt
      241 tctaaaatac gcaaaaaaat aaaattgaag atgttattaa atcgcgatta tttacggaga
      301 tttatataaa taaaaacccc ctgacgaagt cgaagggggt ttttattttg gtttgaggtt
      361 tgcataccta ccgaaaaggt agggcagcaa atcgtcaaac aggtatcagc taattgcacc
      421 ataagggtgc gctgatactc ggctcaaaag agccgaaaga gccgtctgca agacccctcg
      481 gcgagcccac actttgcttg caaagtatag tgggttatac tttacatgga agtaattgcc
      541 ggaaatagtg aaaatgcctc acatttgtgc cacctaaaaa ggagcgattt acatatgagt
      601 tatgcagttt acgggccaag attgtttaag caaaataaga taagttaagg gatgcagttt
      661 atgcacccct taacttatct attaaataat ttatagctat tgaaaagaga caagaattgt
      721 tcaaagctaa tattgtttaa atcgtcaatt cttgcatatt ttaaggcttg ataaaattgg
      781 tttttggaaa atagttttat gtattctttg ttaacccatt tcataacaaa attttcatat
      841 tgttttctat ctttgagtga tatttttgaa gggtgtcttt ttaatacaat taacgagcta
      901 ttaa
//
LOCUS       DACDLI010000182          883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDLI010000182 DACDLI010000000
VERSION     DACDLI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.190"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(74..682)
                     /locus_tag="IV353_002787"
     CDS             complement(74..682)
                     /locus_tag="IV353_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9010937.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT          248 a          153 c          157 g          325 t
ORIGIN      
        1 ttatttttca cacaaaaaaa ccgagaactt gaataaacaa gtttctcggt ttcatcattt
       61 tccttttatc ggcttagtac cagccgtttg ctaaccagaa tgttttagca gcatcccatg
      121 aaccataacg agatgttaca tattgttctg ctacacgttc ttgattggca gctgaatagt
      181 cgccgtttaa gtatgaagca tctaattgat aacggccgat gtaacgtccg ttagtagctg
      241 tgtatgaacc gcctgattct ttttgtgcga tccattcttt tgcagagctt gaagttggcg
      301 ctgttgcagc agctggggca gcaggagcta cttcaactgt ttcaacaggt gcaacgtatg
      361 tatgttcttg cgttgctgtt tcagttgtag atgctgtatt ttctgttgtt ttgttttctg
      421 ttgttgctga agaatcattt gaagttggga ttgttaattg ttcaccaaca taaatgcggt
      481 tgatgtttgc gatcgaattt ttttcagcaa ttgcatcgat cattgagttg tctcctgcaa
      541 atttttgtga gatcttagat aatgaatcac ctgattttac tgtataaact tcatcagcgt
      601 gagcatttgt agttcctaca aacattgcag tagcaccagc agcaaaagtt gtacctaaaa
      661 caattttctt aaatgaattc atattaaact cctttttttc tccgtttttt attctttcga
      721 cgctattaac tttatcattt agaaatattt cataggtaaa agaaacatga catttaatga
      781 caaaataacg aattatttaa tatttgtaac atttaaacaa atattttttt gatacaaaac
      841 cataaaagcc ttgttgtata agcaaagtaa tcgtttttct atg
//
LOCUS       DACDLI010000183          881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDLI010000183 DACDLI010000000
VERSION     DACDLI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.191"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(224..454)
                     /locus_tag="IV353_002788"
     CDS             complement(224..454)
                     /locus_tag="IV353_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010938.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          309 a          136 c          122 g          314 t
ORIGIN      
        1 tcagtcacga tatcaaacaa aaaatatacg tctttcttca ttctagtttt cgcagcaggg
       61 attttaaagt agttcttatt agaatagtag agattaatta ataagctatc ttcgattgct
      121 aaaaagaaaa cttctgaatc ccacacctta taaaaatctt tgacaaatct attcgaagga
      181 tcaaatttaa accataattg cgtctcatta aaaagcataa ccattactcc gatctatttt
      241 taaacttagt ctctacctct aatatatatc gagtttttat tttgccttca gagaatactg
      301 tttctttttt tgcagttaca ggttgtttat tttcggaaaa agctaatata gctaaaattg
      361 aaacatccat ctggaattta tcttttttac tgctttgatc ataaaagtct gcatattcat
      421 cactgatatt ttttctaata aattcttcca tcataaaaat cacctcaaaa cgattatacg
      481 aacttacgtt cttgttgtaa agcgatattt gaggggcaaa aaaggggcat aatttgactg
      541 atattaagtt agctattgat atatgaatga catacaaaaa tataaaatct tttaaaaatg
      601 cttatatatc aatgtttttg ccattctttt atatatctgt tatatgtcaa aaaaatgtgt
      661 tcaatttcaa tttgtatttg aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
      721 aagaacagcc aatcaaaaac agcttctcat gtttttgatt tggttggact tattaccgca
      781 gatataagct tttgagcacc gtgaataaag aggttatgaa atcaacgctc taacctgagt
      841 attagagaaa aaaacgcaaa acatatcatt tatgttttgc g
//
LOCUS       DACDLI010000184          867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDLI010000184 DACDLI010000000
VERSION     DACDLI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.192"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            34..>867
                     /locus_tag="IV353_002789"
     CDS             34..>867
                     /locus_tag="IV353_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9010939.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QR"
BASE COUNT          251 a          174 c          170 g          272 t
ORIGIN      
        1 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
       61 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      121 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      181 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      241 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      301 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      361 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      421 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      481 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      541 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      601 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      661 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      721 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      781 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      841 cttaaaggaa attcccactc tcaacga
//
LOCUS       DACDLI010000185          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDLI010000185 DACDLI010000000
VERSION     DACDLI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.194"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..467)
                     /locus_tag="IV353_002790"
     CDS             complement(<1..467)
                     /locus_tag="IV353_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9010940.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATIS"
     gene            complement(484..>863)
                     /locus_tag="IV353_002791"
     CDS             complement(484..>863)
                     /locus_tag="IV353_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9010941.1"
                     /translation="VFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISESYHITLVE
                     WNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMFWSKSFCL
                     LTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          257 a          140 c          141 g          325 t
ORIGIN      
        1 gatattgtag cggatttgat aatccctttt ggttgtctat gaagcttaat tcttactaat
       61 gacttcaatt taggaacttt gatgaattta ctatcaatca aagcaactgt accattttga
      121 ttattcgttg tataactctg aacaggattt ttcttacttt tgaaacgtgg aaatccaacg
      181 gatttatccc gaaaaaaatt cttgtatgct ttatctaaat taagttgggc attggctaaa
      241 gcaaggctat caatttcttt caaaaacgga aattctttct tgtatttagc tggtgtcgga
      301 aaagtcattt ttttagaaga atcattttta acttcttcat acgccttttt tcggtcatca
      361 agcattagat tgtagacctt acggacacaa ccaaaagatt tggcaaagaa gatttcttgt
      421 tcctctgttg gatagattct gaatttgtat gcttttagtt gttccataaa gttcacctac
      481 tttctattta tgattgtttc cttgattttg gatatacttc ttaatcacat caattggtgc
      541 gccaccagtt gtcagaagac aaaaactttt agaccagaac atttctttcc aaagaaattg
      601 tttgactctt ggaaagtcac gttttatcaa tcttgagctg gcacttttat aggcattgat
      661 gaattttgtc aattctgttt taggttgagc tttgaacata atatgaacgt ggtctttatc
      721 atgattccac tccactaaag taatgtgata cgattctgaa attctttcaa aagtagtttt
      781 ggcataatca gatatttcat catcaatcac ttgtctacga tatttcgtta ctaaaacaag
      841 gtggtaataa agaagaaata ctg
//
LOCUS       DACDLI010000186          851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDLI010000186 DACDLI010000000
VERSION     DACDLI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.195"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(100..>851)
                     /locus_tag="IV353_002792"
     CDS             complement(100..>851)
                     /locus_tag="IV353_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9010942.1"
                     /translation="RGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLV
                     LLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISA
                     GRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNG
                     FLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVE
                     AHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          324 a          149 c          101 g          277 t
ORIGIN      
        1 ataaataaat ctccttttga ttctcggtaa aaattttgat aaacatttaa tatattaatc
       61 aaatgtaaag gagtccaagc aatgaaagct gtcttcatat taattctcct tttttaaata
      121 caagtaagaa atgcttccca aataatacca gaaaacaatt gaaatgacat cgcgcttatt
      181 gaaaagtaca tgagcctcca ccatttcatc tataaaaata gatagtataa aaattaaaac
      241 aataccaaga aacgcataat tcactttact tctaaaaaat gttttgagta aactaaagat
      301 atttaacaca tagaaagcat agatacatat aaaagcaatt ataccacttg atacaagtac
      361 ttgaagaaat ccattatgca tgtttccatg tgctcttttt agttcattca catctgtagt
      421 atcaagatct acttttttca aaaattttgc atcgctattc ccatagaaat cggtctctcc
      481 ataaccaaat aacaaagttg gtcttataac tttaagcgca gctttccaaa tcgtaaaacg
      541 accagctgaa atttcggagt tatcactaga atgctgtaat attacttctt tttctggaag
      601 tggagtttta tctgattcat ttgactctat aacttctact tttctatcaa tcgatacagg
      661 aattgttgct aaaaaatcct tagcataatt agtaccaaaa gaaaaatata ccaatagaac
      721 taataatatg cttaccatcc atttgatttt tacaggtagt acttctctaa tgctctttct
      781 aaattttgaa ttggcaaaca acaaaagaaa taatactaaa aaagaacaaa ttactacctg
      841 ggcacctcta g
//
LOCUS       DACDLI010000187          849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDLI010000187 DACDLI010000000
VERSION     DACDLI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.196"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>849)
                     /locus_tag="IV353_002793"
     CDS             complement(<1..>849)
                     /locus_tag="IV353_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9010943.1"
                     /translation="GTLELHVPRTRDGKFSPTIFERYQRSEKALIAAMIEMVISGVST
                     RKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGSEYPFFMCDALYMKVR
                     ENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQRGLFGVKLVIS
                     DAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKSIFKASELELT
                     RERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTNVLERLNEEI
                     RRRERV"
BASE COUNT          257 a          200 c          140 g          252 t
ORIGIN      
        1 atcactcgtt ctctccggcg aatctcttcg ttcaaccgtt ccaacacatt ggttgtacga
       61 atccttcggc ggatattttc aggaaaagac aaaatttgaa tagcatcttc aaagccgttt
      121 tctaaaatgt cgcaagcagc acttaatttt gaatcacaac catatttttc caagaagtgc
      181 tcttttcttt cacgtgtcaa ttcaagttca gaggctttaa agatactctt caactcgtct
      241 tttacatcag aagaaacttt ctttggcagc ttatcaaaaa tatttctcaa aaagtgggct
      301 tggcatcttt gccagctcgc acctaaaaag ttcttgcgaa cagccttaac taaccctttg
      361 tgtgcatcag aaatgaccag ttttacaccg aacaaacctc gttgaacgaa cgattgaaaa
      421 acggtatccc aattatcttc cgattcgccg tcttgaacat caaaaccaag aatcgtacgt
      481 cgtccatcag aatcaatgcc gataccaata tatacacctt ttgagacaat acgatgattt
      541 tcccttactt tcatatacag ggcatcacac atgaaaaatg gatattccga gccttctagg
      601 ctccggtttc tccactcaaa aacaaatgga tccaactgtt tcatcagatt tgaaacgaat
      661 gatttagaaa cagtcgcacc atccgttagc agttcaactg ttttagttac tttgcgagtg
      721 gagacaccag aaatgaccat ttcaatcatg gcagcgatca aagctttttc gcttcgctga
      781 tatctttcaa agatagtagg actaaactta ccgtctctgg ttcgtggaac atgaagttct
      841 aacgtacca
//
LOCUS       DACDLI010000188          778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDLI010000188 DACDLI010000000
VERSION     DACDLI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.198"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            84..284
                     /locus_tag="IV353_002794"
     CDS             84..284
                     /locus_tag="IV353_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9010944.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            296..622
                     /locus_tag="IV353_002795"
     CDS             296..622
                     /locus_tag="IV353_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010945.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
BASE COUNT          329 a           82 c          146 g          221 t
ORIGIN      
        1 aattgatatt actcgttttg tggtttaaaa tgtgcttatt aagataaaaa ccacaaagtg
       61 aataatttga aaggaggcgc tgaatgataa ctcttaatga tcaattcatt agaagcttaa
      121 gaagacatag agccgatctt atacttacta aaaacgatgc agcaaaattg ataggtataa
      181 atcggaaaac atatgtcaaa atcgaaaatg ggagcaaaga aagtattaga gcatctactt
      241 atcaaaagtt ggtaaattgg ttacttaatg atttgaaaat ctaaataaat acaacttggc
      301 gagttggctg attgaagaaa tagaaaatga gaggagaaaa ataatggatg caggaattac
      361 agtaatgtta gataaacaac aaacgaatca attgaaaaat tatgtttttg aaatgacaaa
      421 agaggctatt gatcaagcaa gaattgatac cggattagaa agaccatttc taaaaggaaa
      481 agagatggct aaatatttga atgtatcata tacaactttt ttgaaattta aaagaatggg
      541 attgccagtt attttattag aaaaaatgga gttgttttca aaagaagaat gcaaaaaatg
      601 gatattgagt catcaaattt aaaaaacacc tcgtttttta aatttgatga agtagaaaaa
      661 attaaaaaga gtgttcccgt cagtttgcag atcgggggaa cactcaataa taaagttctg
      721 agaaaacttt atttgaatct attatagcaa ataaaatcag aagcaggtgt gacaatga
//
LOCUS       DACDLI010000189          766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDLI010000189 DACDLI010000000
VERSION     DACDLI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.201"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            660..>766
                     /locus_tag="IV353_002796"
     rRNA            660..>766
                     /locus_tag="IV353_002796"
                     /product="16S ribosomal RNA"
BASE COUNT          315 a          124 c          135 g          192 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactct cgatgaagta gacctttgaa aactgaacaa
      541 agtaagacaa accaaatgtg tagggcgtct tgattcaatt caagacaaca aacattttta
      601 acaagcaagc aatatgctag caaacaaatt gagcttaaca atcgcaagat tgttcgaact
      661 ttttatgaga gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa
      721 gtcgaacgct tctttttcca ccggagcttg ctccaccgga aaaaga
//
LOCUS       DACDLI010000190          764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDLI010000190 DACDLI010000000
VERSION     DACDLI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.202"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..660
                     /gene="tnpB"
                     /locus_tag="IV353_002797"
     CDS             <1..660
                     /gene="tnpB"
                     /locus_tag="IV353_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9010946.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          275 a          131 c          141 g          217 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttcta ccggtcaaaa atttgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgagaatg gacttgccct atttgtcata ctcatcatga ccgagatatc
      601 aacgctagta tcaatatcct gaccgaaggt ctaaggctac actctatggg attggcttag
      661 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
      721 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaa
//
LOCUS       DACDLI010000191          763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDLI010000191 DACDLI010000000
VERSION     DACDLI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.203"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..660
                     /gene="tnpB"
                     /locus_tag="IV353_002798"
     CDS             <1..660
                     /gene="tnpB"
                     /locus_tag="IV353_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9010947.1"
                     /translation="YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFT
                     SKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNK
                     LSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKV
                     DKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          283 a          114 c          142 g          224 t
ORIGIN      
        1 tatatttctt tgttgtgtaa agaagaaatt atcgaattac ctaaaactaa ttccacaatt
       61 ggtattgact taggtattat ggactttgcg attctttctg atggacaaaa aattgataat
      121 aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg aacaacgcaa attgtctagg
      181 cgtgctttat tggctaaaaa taaaggtacc aatctatttg aagctaaaaa ttatcaaaaa
      241 caaaaacgca aagtagctag attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat gatcaaaaat cacgatatta tctgtattga agacttaaac
      361 acaaaaggta tgttgcgaaa tcataaatta gctaaaagta tttctgatgt ttcttggtca
      421 aagtttgtga ctaaattaca atacaaggct gactggtatg gacgaaaaat catcaaagta
      481 gataaatggt ttccgtctag tcagatttgt tcagagtgcg gacataaaga tggcaagaaa
      541 tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata ctcatcatga tcgtgatatc
      601 aatgctagta tcaatattct gaccgaaggt ctaagactac attctatggg attggcttag
      661 atccaatcat caagaaccgt aggaactacg gggatagctt ggtaaataag agacaccgct
      721 gttagtaaag aaatacgcta tcaagtatgc tctattccca aga
//
LOCUS       DACDLI010000192          728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDLI010000192 DACDLI010000000
VERSION     DACDLI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.204"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>728
                     /locus_tag="IV353_002799"
     rRNA            <1..>728
                     /locus_tag="IV353_002799"
                     /product="16S ribosomal RNA"
BASE COUNT          177 a          164 c          228 g          159 t
ORIGIN      
        1 taactacgtg ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gatttattgg
       61 gcgtaaagcg agcgcaggcg gtttcttaag tctgatgtga aagcccccgg ctcaaccggg
      121 gagggtcatt ggaaactggg agacttgagt gcagaagagg agagtggaat tccatgtgta
      181 gcggtgaaat gcgtagatat atggaggaac accagtggcg aaggcggctc tctggtctgt
      241 aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca
      301 cgccgtaaac gatgagtgct aagtgttgga gggtttccgc ccttcagtgc tgcagctaac
      361 gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag gaattgacgg
      421 gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca
      481 ggtcttgaca tcctttgacc actctagaga tagagcttcc ccttcggggg caaagtgaca
      541 ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag
      601 cgcaaccctt attgttagtt gccatcattc agttgggcac tctagcaaga ctgccggtga
      661 caaaccggag gaaggtgggg atgacgtcaa atcatcatgc cccttatgac ctgggctaca
      721 cacgtgct
//
LOCUS       DACDLI010000193          705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDLI010000193 DACDLI010000000
VERSION     DACDLI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.205"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            38..>705
                     /gene="aac(6')-Ie"
                     /locus_tag="IV353_002800"
     CDS             38..>705
                     /gene="aac(6')-Ie"
                     /locus_tag="IV353_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP9010948.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNE"
BASE COUNT          291 a           52 c          131 g          231 t
ORIGIN      
        1 agtatatgta tatgattatg aaaaaggtga taaataaatg aatatagttg aaaatgaaat
       61 atgtataaga actttaatag atgatgattt tcctttgatg ttaaaatggt taactgatga
      121 aagagtatta gaattttatg gtggtagaga taaaaaatat acattagaat cattaaaaaa
      181 acattataca gagccttggg aagatgaagt ttttagagta attattgaat ataacaatgt
      241 tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg attatcatta
      301 tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc caaattattg
      361 gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga aaaaagaaag
      421 aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa taagggcata
      481 ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac acgagggcaa
      541 aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa atgttaaggc
      601 aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta ttgaaataat
      661 cggtagtggt tatgatagtg tggcatattt agttaataat gaata
//
LOCUS       DACDLI010000194          703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDLI010000194 DACDLI010000000
VERSION     DACDLI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.206"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(359..>703)
                     /locus_tag="IV353_002801"
     CDS             complement(359..>703)
                     /locus_tag="IV353_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP9010949.1"
                     /translation="QHREPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHK
                     LFVRGYSAIQHPHKPDVTERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIII
                     QFASSLELVAFK"
BASE COUNT          233 a          119 c          121 g          230 t
ORIGIN      
        1 acattaagac aaagcgcaaa aattgaaatt attaatggaa tcatttctct tcacttacct
       61 ttagttatcg tgtgaataat taaaaaaaca atagacagca acggatgata gataataaga
      121 acaatttaga aggagttgaa attcacatcc ttattcttaa tatttccgtt gctgcctatc
      181 gaagcttaat taaacgatga gggagatttc ctcccttaca ttttattttg tcgatcctgt
      241 ttcctaattt ttcgacacta tcataataca acgttgaata ggtaagtgat tggtataaaa
      301 aaggtataaa atggaaacca aattggtaat aaaagggtat aaaaagtgta aaaactggct
      361 acttgaaagc aaccagttct aacgatgaag caaattggat gataattctg tttgattcta
      421 ctttaaccga ttcttcgcta gtattattcc tttgagcagt tacataaatg ggcagaccat
      481 tgatataacg atcatagaat atcttcttgc gcctttcagt cacatcaggc ttatgcggat
      541 gctgtatcgc tgaatagcct cgaacaaaca atttatgcag atactcaaat tcttcctgtg
      601 cttcttcttt atcgatcagc attctttctg cttcaaatat atgatcagct gtagaaggtg
      661 gaaccaagga ataagatgct gtcacttttg gttctcgatg ttg
//
LOCUS       DACDLI010000195          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDLI010000195 DACDLI010000000
VERSION     DACDLI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.207"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..258
                     /locus_tag="IV353_002802"
     CDS             <1..258
                     /locus_tag="IV353_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9010950.1"
                     /translation="EIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMP
                     ESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            259..>695
                     /locus_tag="IV353_002803"
     CDS             259..>695
                     /locus_tag="IV353_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9010951.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT          265 a           78 c          127 g          225 t
ORIGIN      
        1 gaaattcatt tagattattt tggaagagtt cctacaagaa aatttcaatt gtttacttta
       61 gatgaatgta tcaatgttga cttattaaat aataaaataa gttttgaaga aaacattgtt
      121 aaagaaatgc ctgaatctgc taatgatatg tatgagaagg agttgaaata tttctttgaa
      181 aacgtagtaa caggcaaaga aaactggaat gatttatatc atgcaaacga agtattgaaa
      241 atagctgagg agaaataaat ggaaatttta tttactattt gtggtagagc tgggtctaag
      301 gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc ccctagtcca tttcgcttta
      361 gcaatgatag atatctatca acaaaaacat cctgaacata attgcacgac ttgtttgaat
      421 acggacagta atgagttagt agaattgacg actaagttaa atccgtctgt gaaaattgta
      481 gaacgtaaac ctgaattagc tacagatact gtagggaaaa ttgaagtgat acgtgattca
      541 ttcttacaac tagaaagaga agataatata tttgatttaa ttatagattt agatttgact
      601 tcacctttac gtgaagtttc taatcttgaa gaattaatag atgaacatct aaaaaattta
      661 gataaagacg tctacttttc agttgtttct gcaag
//
LOCUS       DACDLI010000196          694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDLI010000196 DACDLI010000000
VERSION     DACDLI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.208"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..>694
                     /locus_tag="IV353_002804"
     CDS             73..>694
                     /locus_tag="IV353_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9010952.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQ"
BASE COUNT          248 a          124 c          129 g          193 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caat
//
LOCUS       DACDLI010000197          683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDLI010000197 DACDLI010000000
VERSION     DACDLI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.209"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(80..337)
                     /locus_tag="IV353_002805"
     CDS             complement(80..337)
                     /locus_tag="IV353_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9010953.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(330..599)
                     /locus_tag="IV353_002806"
     CDS             complement(330..599)
                     /locus_tag="IV353_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9010954.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          212 a          130 c           83 g          258 t
ORIGIN      
        1 tgctaagaaa cgtaatgtaa tgaaaaaaat agaggtcaaa aacttaaaag tttctgaccc
       61 ttcccttact tccaattggt taatagtgat cttttgcaga ataaataaat atcgtttcat
      121 tttcaactct atatatcagt ctatgttcat ctgtaattct tctggaccac tttccagata
      181 aatcatgctt taatggctca ggttttccta atccagcaaa gggagaacga tcgatatctt
      241 ttattaactt gttaatcttt tttatattgc ttttgtttcc ttgctcatgc caataaagat
      301 aatcatccca agcatcatca gaccaagcct taatcatcag attcaacctc gattaagtca
      361 tgtgttttaa atgcaccttt ggagaattgt tcatctcctc gacgaatttt ttccatgacg
      421 taattattag aaagtgttct caacgtttct tgcatagaat cataatctct ttttgataat
      481 acaacaactg tatcttctac atctttactt gttacaataa gtgtttcagc atcctcatta
      541 acttgtttca tataactacg taaattttgg cggaaatttg aataagctac tgcttccatt
      601 cctttcaccc cttatttatt tctataaaac aattgtacat tatattgtac aattaagcaa
      661 atgattaata ttctaacttc tta
//
LOCUS       DACDLI010000198          680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDLI010000198 DACDLI010000000
VERSION     DACDLI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.210"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          241 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatta
//
LOCUS       DACDLI010000199          642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDLI010000199 DACDLI010000000
VERSION     DACDLI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.211"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..>642
                     /locus_tag="IV353_002807"
     CDS             6..>642
                     /locus_tag="IV353_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9010955.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLG"
BASE COUNT          208 a          115 c          128 g          191 t
ORIGIN      
        1 gctatatgaa tgattctatc aaaaaaatgc tgagaataac agagaaagat ttgatgatta
       61 cagaggtctc ttacgagacc cttcagaaga aaaagacgtt ggtcgtcgat gctgttctct
      121 cgcctactcc tcgtgcttgt agaagttgtg gttctactgt ggtagatgga aacgggaaag
      181 caattatagt gaaaaatgga aaaaaggaaa cgattgtccg ttttgaacaa tacaatcata
      241 tgcctttggt tatgcgccta aaaaagcaac gctatacctg taaaaactgt cgcacccatt
      301 ggacggctca aagttatttt gtccaaccca gacattcaat cgcaaatcat gttagatata
      361 aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa agctgtcagg
      421 tatctttgac caccgttatt cgtacattga aagagtttaa aagctattta ccaaagcaat
      481 ctaagaagat cctccccaga gtattgatgg ttgacgaatt tcgttcgcat gcttccatag
      541 aagataagat gagctttatt tgcgcggatg gcgaaacagg aaaattaata gatgttttgc
      601 ctacgcgtaa attacctcga ttaacaagct atttcttagg tt
//
LOCUS       DACDLI010000200          640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDLI010000200 DACDLI010000000
VERSION     DACDLI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.212"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..283
                     /locus_tag="IV353_002808"
     CDS             <1..283
                     /locus_tag="IV353_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9010956.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          214 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt
//
LOCUS       DACDLI010000201          604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDLI010000201 DACDLI010000000
VERSION     DACDLI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.215"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          181 a           97 c           79 g          247 t
ORIGIN      
        1 catcatattt gattactttt ttaagataat gtagcatctt tttatccaat aacgcaattg
       61 tttttaagga taaatttctt aagatggtac atttcaaaat ggcatttgac atatgaaaca
      121 aaaaagaaag aaaagagcat aagggaaaac tgatagcacc ttcctacagg aaggtggcta
      181 agttggctgt gccaactggt tttctttcaa aatcatttca taatttttga tatcataaaa
      241 aaatccattt ttgacctatt ttcggtcata atttagtacc tacttttggt catagtttcg
      301 tccgtagttt tggtcatagt gactaaggct tttctgttca caaaatgggt ttaaatttgc
      361 tttctttaac acttcattta tatcatagca attataatgt ttacacaaca tcattcctaa
      421 ttttaacggt tgttcctctt tatatttttt aagttctaca tttttttgcg tttaattact
      481 tttctctttt ctttatattc cgctccaact ttctctttaa tagtgacaat agaattacat
      541 atttttgtta aacttcctta aaaaaattgg atctcgatta ctcaaatttg cttgcaggct
      601 tttc
//
LOCUS       DACDLI010000202          578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDLI010000202 DACDLI010000000
VERSION     DACDLI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.216"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..496)
                     /locus_tag="IV353_002809"
     CDS             complement(<1..496)
                     /locus_tag="IV353_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010957.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIY"
BASE COUNT          183 a          105 c           60 g          230 t
ORIGIN      
        1 tatatatttg tcctgctaaa tatacttctc ccagatatgt gtgttctttt ggtttaaatg
       61 tttcaaataa atgaatcttt acaaaattac tttttgaatt ggctaaaact ttattttgat
      121 tcttatccaa tacttgatcc ccttttagcc ccataccggt atattgtaaa ataccattta
      181 accacttatc tttataaaca ttacttcctg tttgatcact aaacagtaca agagtattag
      241 tttgatgaga tcttctcatt ccaccctgtt tggaacattt aaacttctct gaaatttctt
      301 tgttagtata tactctacca acttctaact cacctaatat gtttttagac caattttctc
      361 gccatgtaat aatcttatca agattaaaca tttctccctc ttttggcatg ccatttctaa
      421 ttaaattagc taatacacca gtagaaatat ctaatttttt tagtaattct ttcttagtaa
      481 tcaatgttgg attcataaca atgcagctcc taatgatatc aatataattg cattagataa
      541 cttgaaaaat cgaaaaaaac tcattttgtg tcaagtta
//
LOCUS       DACDLI010000203          545 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDLI010000203 DACDLI010000000
VERSION     DACDLI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.217"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..123
                     /locus_tag="IV353_002810"
                     /pseudo
     CDS             1..123
                     /locus_tag="IV353_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            199..>545
                     /locus_tag="IV353_002811"
     CDS             199..>545
                     /locus_tag="IV353_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9010958.1"
                     /translation="MDHFKGKRFQKDVIIVAVGYYLRYNLSYRDVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYTIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYA"
BASE COUNT          179 a           92 c          117 g          157 t
ORIGIN      
        1 ggaaaggaat ttgataacca aaccattcat gagattctga atggttttgg aattactcgt
       61 tcattgagta ggaagggttg tccgtatgat aatgccgttg cggaatcaac ccataaatct
      121 gtcggttctg ttgcaaagtt ttctgataaa ctgattttaa ggtaaaatga atggaaaaga
      181 tagcttggag gaatcaagat ggatcatttt aaaggcaagc gatttcagaa agatgtgatt
      241 attgtcgccg taggctacta ccttcggtat aatcttagct atcgtgacgt ccaagaaatc
      301 ctatatgacc gtggcattaa cgtttctcac accaccatct accgctgggt acaggaatat
      361 ggaaaactac tttacactat ttggaaaaag aaaaataaac aatccttcta ttcgtggaag
      421 atggatgaga cctatattaa aatcaaaggg aaatggcact atctttatcg agcaatcgat
      481 gcagatggtt taaccttgga tatttggtta cgtaaaaaac gggacacaca agcagcctat
      541 gcttt
//
LOCUS       DACDLI010000204          519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDLI010000204 DACDLI010000000
VERSION     DACDLI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.218"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          188 a           61 c           73 g          197 t
ORIGIN      
        1 ataaagaaga cttagatagt cataagttaa ttagttatcc aattatagaa aaacaacaaa
       61 ttaaatatat aaattagtta atgtgttgtg attgattatt taatatagat ataatacaaa
      121 aaggcaaaga tattttcaaa acattttttc ttgatgtgat atccttgtct tttttgtaat
      181 gtttttaact tattgctttt gcaaaattta tttacctgtt ttctggatca acgatagtgt
      241 atattatgct atccttgttt ggattttagg acgtttaaag aggcactttt ttacataact
      301 taatccatag accttttaag catgctgtga gataataata aagaagagtt taaagcgcac
      361 cccaaaccac ttccccataa gtgtattacg ctttaaactc ttttatattt gaattcacta
      421 agaagcatac catatttttg aaaaaaagtg agaaaaaagg cttataatta ggatttagag
      481 taattaatta gatgtttatt ttataggact gatactata
//
LOCUS       DACDLI010000205          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDLI010000205 DACDLI010000000
VERSION     DACDLI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.219"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV353_002812"
     CDS             complement(<1..132)
                     /locus_tag="IV353_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9010959.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACDLI010000206          461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDLI010000206 DACDLI010000000
VERSION     DACDLI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.221"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>461
                     /locus_tag="IV353_002813"
     CDS             <1..>461
                     /locus_tag="IV353_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9010960.1"
                     /translation="IIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSS
                     NLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKK
                     SYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKG"
BASE COUNT          149 a           84 c           84 g          144 t
ORIGIN      
        1 tgattattgc ttgtgttata tggggcataa ttaatggcta tactagccaa agagccacgt
       61 atagagcggt ttgttccgtc ttatttccta atggtgactt tcctagtagg agtcgattca
      121 ctcgcttaag ttcaaactta gcttacacta tcaagattat tcgatacttt ttcatcaaga
      181 agctcaccaa aggtgaacta gtcggaatta tagatagttt tcctagtcca ttatgtaaac
      241 cagttagaaa tagacaagca aaactattga atcaaatagc caaagttggc tataactcaa
      301 caaaaaaatc ttatttttat ggtcttaaaa ttcatatgat cgttactaag actggctttc
      361 cgattactta ctcaatcacg aatccaggtg tccatgacgt gaaagtgtta gagactttat
      421 cggaagaagc gaatcttccc aatatcctag gggacaaagg a
//
LOCUS       DACDLI010000207          450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDLI010000207 DACDLI010000000
VERSION     DACDLI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.222"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(35..271)
                     /locus_tag="IV353_002814"
                     /pseudo
     CDS             complement(35..271)
                     /locus_tag="IV353_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          151 t
ORIGIN      
        1 tagaaggact gaccttctgt ctcagatttt tttgttattt cacttgaatc aattgattga
       61 tcaaaaaaat cctgattttc atgttctcaa atgatttaaa tccataagat aatcgtttca
      121 gtgtcttgat atggggaatc ttggcctcta ttttgccatt agaatacggg taaatcatag
      181 cattgtaaat accttcttca tacgttaaaa ggttttgaag tttcgtgcga agttgtgcat
      241 caagtgtttc aggaagattt tttaataacg ccgtttgtat aattaagcga gacaaataaa
      301 aaagccattt atgttacact ccaattgtaa tacccgctaa agcatacaag gagagtgaac
      361 ataaatgact tatacccatc ttacaccaaa cgagcttgta atgatagaag catattttca
      421 tcaagaaact ccggttgcta tcgttgcgaa
//
LOCUS       DACDLI010000208          387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDLI010000208 DACDLI010000000
VERSION     DACDLI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.224"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..387
                     /locus_tag="IV353_002815"
                     /pseudo
     CDS             54..387
                     /locus_tag="IV353_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          157 a           72 c           75 g           83 t
ORIGIN      
        1 gacagaaaaa ataaggtgtg tagaataata aaaaacacac tggaggattt ttcatgtcaa
       61 gacgtcaacg aagaacctat tcaaaagaat tcaaacaaca aatcgtcgat ctctatctcg
      121 ctggtaagcc tcgcgcagaa attattcgag agtatgagct tacgccttct tctttcgata
      181 aatggatgaa gcaagcacaa tcaacgggct cattcaaaga aagagacaac ttaacaccag
      241 aacaagcaga attgatcgca ctaagaaaga aaaataagca actcgaaatg gagaatgata
      301 ttttaaagca agcggcgctg atattcggac gaaaagacaa gtaattgatg ccaacaagca
      361 taaatattcc atatcagcga tgtgcaa
//
LOCUS       DACDLI010000209          382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDLI010000209 DACDLI010000000
VERSION     DACDLI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.225"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..369
                     /locus_tag="IV353_002816"
     CDS             <1..369
                     /locus_tag="IV353_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9010961.1"
                     /translation="DDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLL
                     TFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFL
                     NRSHKGFQQVTDTLVSMFTE"
BASE COUNT          145 a           70 c           57 g          110 t
ORIGIN      
        1 gatgacttta aggctgttta tcaagctaac tcaaaagaag aagcgaatac cttcttatcc
       61 ggcatgattg agaaatggaa gaaaaactat cctaaagtga cgcagtcact catagaaaac
      121 caagacttat taacttttta tgattttcca cctagcattc gtagaaccat ttactcaacc
      181 aatctaatcg agtctttcaa taagcaaatt aaaagataca gccgtagaaa agagcagttt
      241 caaaatgaag aatcactaga acgctttcta gtcagcattt ttgatacata caatcaaaaa
      301 tttctaaaca gaagccataa aggttttcaa caggtaaccg atacattagt ttcaatgttt
      361 actgagtaac taattatttt gc
//
LOCUS       DACDLI010000210          374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDLI010000210 DACDLI010000000
VERSION     DACDLI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.denovo.226"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>374
                     /locus_tag="IV353_002817"
     CDS             <1..>374
                     /locus_tag="IV353_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9010962.1"
                     /translation="EEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          129 a           76 c           69 g          100 t
ORIGIN      
        1 aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt
       61 tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct
      121 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg
      181 attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt
      241 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat
      301 tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact
      361 tttacaattt caaa
//
LOCUS       DACDLI010000211         5201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.124, whole genome shotgun sequence.
ACCESSION   DACDLI010000211 DACDLI010000000
VERSION     DACDLI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.124"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..185
                     /locus_tag="IV353_002818"
     CDS             <1..185
                     /locus_tag="IV353_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9010963.1"
                     /translation="KAQKKIEHCEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLC
                     NITTETKEVNNNGTKK"
     gene            169..798
                     /locus_tag="IV353_002819"
     CDS             169..798
                     /locus_tag="IV353_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9010964.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1152..2630
                     /gene="msr"
                     /locus_tag="IV353_002820"
     CDS             1152..2630
                     /gene="msr"
                     /locus_tag="IV353_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9010965.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2677..3123
                     /locus_tag="IV353_002821"
     CDS             2677..3123
                     /locus_tag="IV353_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9010966.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3322..3837)
                     /locus_tag="IV353_002822"
     CDS             complement(3322..3837)
                     /locus_tag="IV353_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9010967.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3954..5155
                     /locus_tag="IV353_002823"
                     /pseudo
     CDS             3954..5155
                     /locus_tag="IV353_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1606 a          875 c         1120 g         1600 t
ORIGIN      
        1 aaaaagccca gaagaaaatc gagcattgtg aaatggacca taaagataca ggttccgtgc
       61 tatccccggc tttcagcgat gtagaaatga aacaatttct aacactttta gataaattga
      121 atacccaact ttgcaatata acaacagaaa cgaaagaggt taataataat ggaacaaaaa
      181 aatgattttt caacaattat gactggtaga agatccgttc gcgtatatga tgaaacatac
      241 aaaatcccac acgaagaaat gctggaaatg atcaaagaag cgacgactgc accttcttct
      301 gtcaatatgc aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg
      361 cgtcctttga tccgtttcaa tacgagacaa aatgatactt cttctgcaat ggtcatgatt
      421 tttggcgata tgcaatgtta cgaatatggc gaagaaatct ataaccaagc ttacgaatca
      481 gggaagatgc caaaagaagt cagagatcaa caattagctg cgatcattcc ttattacaag
      541 agtttttcaa gagaagaaat gaacgatgta gtcaaagtcg attctagctt agcggctatg
      601 caattcatgt tagttgcgcg tgatcatggc tatgaaacaa atccaattgg tggattcgaa
      661 gcagatcagt tggcagaggc atttggtttg gataaagacc gttatgttcc agttattatt
      721 ttatcaatag gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc accagaaaag
      781 atcactacgt tcgaataaaa ggttttcagt tactattcag tagctattgc tttttttgaa
      841 tcgtgtgtgg taacctaata aaaaaggagg tcggaaaaat gactgcatcg atgaaattac
      901 gtttcgaact tttgaataac aactaaataa attcaaaaga catgtataaa caacaatttc
      961 atggatggag tatgtccatt ttttcgaacg acagtagaat aggtgaaagc tatgacagat
     1021 ttggtgattt agtcatagtt atttcgttca gagtaggaaa agatgtgatg cagttttttt
     1081 gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca tgaaatagga
     1141 gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc agctttggca
     1201 accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt atagggatca
     1261 taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt gaactttttc
     1321 ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa ttaacctatc
     1381 tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta agactggaag
     1441 aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg atacgtcttc
     1501 tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta gatagaaaaa
     1561 gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt tttgttagtc
     1621 atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa
     1681 tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg gagaaaaaga
     1741 ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa aaatcgatcc
     1801 aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac
     1861 aaatcagacc ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc
     1921 aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca accaagccgc
     1981 agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac agccgctatc
     2041 ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg gtaaatggtg
     2101 attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga
     2161 caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa
     2221 gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc atcattcgtt
     2281 ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg aatcacttag
     2341 ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga gaagcgaccc
     2401 gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta gatgaaccga
     2461 ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa atatatccgg
     2521 gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct gatgaagttt
     2581 atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa atgtgctacg
     2641 atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa aaaagaagga
     2701 ataattttta caacaatgat gtgttttttg atggtcttgg ggatgagcgc gtataattta
     2761 tggctgcatc aagatcttca tttatcaagt ttgttgattg gtctagtgcc tggattcgtg
     2821 gtggctttca ttttggatgt cttcatcatc ggtgtgctgg cgaaaaaaat tgcctttaaa
     2881 ctaccagtaa atccagaaag caaggtccaa atgatcttaa ctatttcatg cttgatggtt
     2941 ttaggaatgg tgacatgtat gtctttattc ggtgtgatca tggaacaagg aatacctgat
     3001 caattattct ctgcttatat gaatgcttgg aaaatgaatg tgattgccgc tttaccgttg
     3061 cagttgatca tcgttggccc gctttcaaga agtgtgctaa aagcaattca aagtaatgaa
     3121 tgatggaaca ctgttatttg aaaaaaggcc ttggcatgct ttttgtcata gtctcttgaa
     3181 tgcagttcta gaaaaatagt gatccgaacg ataggttttg gcctgtcgtt cggatcgctt
     3241 tttcttttcc aactgtattg ctcttttatt gaagataatt tgctcttcaa tagatatttc
     3301 tagaaaggtt ctgtttatcc cttactcaaa aaactgctgc atttgttctc ttacttgctg
     3361 acaccattgg agttcctgct gacaaagcat aattctgcgc tggatcacaa cataacgtcc
     3421 aaattcttgt ttcttgtcta gcgagaggtc attcaatttt ttttgatttt tttgcatgat
     3481 tttgttcagc tgttgttctc tagtgaatag cagtccagta gctgttgttt tatcgagcgt
     3541 cgaaaaagca tagatttttg ctagaaattc atctttttgc gtaggagcgg gagtttcctc
     3601 tttgatccat tcctctacaa gagcacgtcc ttcatcagtc aagtggtaga tctttttttg
     3661 agagtgctcg tctttgtcca aaacggtcac ataaccctgc tggtctaact tggcaagcgt
     3721 tgtatagatc tgactgtgat gggcttccca aaaaattgtg atcagctgct tcaattcata
     3781 cccagaacgt ggtttggata aaagcacgca taataatata taggctaaag tattcataaa
     3841 aatcctacct ctcgtcttat tttctttatt atatcacagt aaagtttcca cgttttacat
     3901 gtgcatattt acatgtaaaa taaatcatgt atgatgctaa tagagagaaa gggatggaaa
     3961 tattggagaa tcaaatagat atacaagaac aaacaaatga agaaacaaag gggaaaagtt
     4021 cagcgctagt tgtagcagct acctgtatga tcgcttttat gggagtgggt ctagtagacc
     4081 caatcttaaa aacgattgct gcacagttaa acgctacgcc agctgaaacg accctgttat
     4141 ttactagtta catgttagtc actggtgtgg tgatgctatt tacaggtttc ttatctagtc
     4201 ggatcggttt gaaaaaacat tgatgacagg attgtttatc atcgttgtat tcgctggtct
     4261 tggcggatta tctgggaata tcggaatgct gattgggtta cgtgccggct ggggtgtcgg
     4321 gaatgcttta tttgtctcta ctgctttagc aacgatcgtc agtattttag ttggtaacac
     4381 agaaaaagca atcatgatgt atgaagcagc acttggctgc ggaatggctg ttggccctct
     4441 agtcggtggg gtcttgggca gtggttcatg gcgttatcca ttttttgggg tagcattttt
     4501 gatgttttgc ggttttatag ccctagcggt acttttacct gaaactgaga aaccaaagag
     4561 aaaagtaggc ctttgggaag gtgtcaaagc gttaagcaat cataaacttc gttcagttgg
     4621 actaatcgca ttgttatata atttcggatt ctttacttta cttgcgtatg cgccattttt
     4681 attgattggc tattctgaat tagaagtcgg ttttgtattt tttggctggg gtgtgcttct
     4741 ggctattgct tcgattttcg ttgcaccggc tcttgagcgg aagtttacga cttatcggac
     4801 aattttagca gcatttgtct tattcattat ttgtttgctg ttattaggag ctggaacttc
     4861 tatgccagtc cttgtaccga tcagcattgt gttagcagga tttttccaag gaattatcaa
     4921 tacactgttg acgacgattg ctatggaaat accaggtctg caacgaaacg ttgcttcgtc
     4981 cagctatagt tttgtccgct ttttcggtgg agctttagca ccatttattg caggtgtgat
     5041 tggtgaacat attggtgaaa gctatgcgtt ttattttgca ggtgccattg ttcttatcag
     5101 tgtatgttgt atcatttatc accgtaatta ttttgctaca aatgaagaag aataagaaat
     5161 cagaggttgt gaaaaagctg tcctgcatca agtcataaga a
//
LOCUS       DACDLI010000212         4744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.128, whole genome shotgun sequence.
ACCESSION   DACDLI010000212 DACDLI010000000
VERSION     DACDLI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.128"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..985
                     /locus_tag="IV353_002824"
     CDS             <1..985
                     /locus_tag="IV353_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9010968.1"
                     /translation="TGSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVELNMYNGIP
                     HLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKNLENGTKHP
                     TLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIK
                     ANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQGAFISDHEV
                     ERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQ
                     RRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            1215..2282
                     /gene="rlmN"
                     /locus_tag="IV353_002825"
     CDS             1215..2282
                     /gene="rlmN"
                     /locus_tag="IV353_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9010969.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(2475..3578)
                     /locus_tag="IV353_002826"
     CDS             complement(2475..3578)
                     /locus_tag="IV353_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9010970.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            4131..4679
                     /gene="aac(6')"
                     /locus_tag="IV353_002827"
     CDS             4131..4679
                     /gene="aac(6')"
                     /locus_tag="IV353_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9010971.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1570 a          814 c          999 g         1361 t
ORIGIN      
        1 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
       61 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
      121 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
      181 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
      241 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
      301 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
      361 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
      421 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
      481 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
      541 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
      601 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
      661 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
      721 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
      781 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
      841 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
      901 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
      961 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
     1021 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
     1081 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
     1141 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
     1201 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
     1261 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
     1321 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
     1381 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
     1441 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
     1501 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
     1561 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
     1621 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
     1681 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
     1741 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
     1801 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
     1861 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
     1921 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
     1981 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
     2041 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
     2101 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
     2161 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
     2221 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
     2281 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
     2341 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
     2401 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
     2461 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
     2521 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
     2581 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
     2641 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
     2701 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
     2761 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
     2821 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
     2881 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
     2941 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
     3001 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
     3061 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
     3121 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
     3181 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
     3241 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
     3301 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
     3361 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
     3421 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
     3481 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
     3541 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
     3601 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
     3661 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
     3721 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
     3781 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
     3841 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
     3901 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
     3961 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
     4021 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
     4081 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
     4141 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
     4201 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
     4261 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
     4321 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
     4381 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
     4441 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
     4501 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
     4561 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
     4621 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
     4681 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
     4741 actt
//
LOCUS       DACDLI010000213         3620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.139, whole genome shotgun sequence.
ACCESSION   DACDLI010000213 DACDLI010000000
VERSION     DACDLI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.139"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IV353_002828"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IV353_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9010972.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IV353_002829"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IV353_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9010973.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1148 a          553 c          737 g         1182 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaactat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct
//
LOCUS       DACDLI010000214         3620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.140, whole genome shotgun sequence.
ACCESSION   DACDLI010000214 DACDLI010000000
VERSION     DACDLI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.140"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IV353_002830"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IV353_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9010974.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IV353_002831"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IV353_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9010975.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1147 a          553 c          738 g         1182 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaactat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct
//
LOCUS       DACDLI010000215         3281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.144, whole genome shotgun sequence.
ACCESSION   DACDLI010000215 DACDLI010000000
VERSION     DACDLI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.144"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            71..568
                     /gene="dfrG"
                     /locus_tag="IV353_002832"
     CDS             71..568
                     /gene="dfrG"
                     /locus_tag="IV353_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9010976.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(640..2592)
                     /locus_tag="IV353_002833"
     CDS             complement(640..2592)
                     /locus_tag="IV353_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9010977.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2599..2895)
                     /locus_tag="IV353_002834"
     CDS             complement(2599..2895)
                     /locus_tag="IV353_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9010978.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3070..>3281)
                     /locus_tag="IV353_002835"
     CDS             complement(3070..>3281)
                     /locus_tag="IV353_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9010979.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT          996 a          580 c          541 g         1164 t
ORIGIN      
        1 taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag ttaaaaaggc
       61 tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag tgattggcaa
      121 agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa atactacaaa
      181 gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag ccttacctga
      241 cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt gtgaaattgt
      301 tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt ttattttcgg
      361 aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca tcacaaaaat
      421 acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg aatggaatga
      481 ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact attttcatgt
      541 atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga acagtaaaga
      601 taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat aaattatatc
      661 tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca aaaccttttt
      721 atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg taatcgcaaa
      781 ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac cacgagctgt
      841 tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa agctattggt
      901 cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca gagcagataa
      961 atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg gatgtttgat
     1021 agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct cttcgtggaa
     1081 ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta gaaactttgt
     1141 tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc cgaattcttt
     1201 taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat gtgcatcgct
     1261 caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga agtcatcatc
     1321 actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat agtgagcagt
     1381 tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat tagtaatttt
     1441 ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg gttcttgtgg
     1501 ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat ggtcttcttt
     1561 gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat aggcaacatc
     1621 agaacggaac acatatcttg ataagacctc agcagataca actatatacg tttgcatatt
     1681 aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga cgttatttag
     1741 gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg tttgaagggg
     1801 ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact ccagcttatg
     1861 atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg aaacccttcc
     1921 aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat aagtagtggt
     1981 ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt gatagcgttg
     2041 agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga atggggttga
     2101 ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat cttggatgct
     2161 attgatttct ataaggcgtt taatttcttc aacaacagac caattagata aaggggttac
     2221 tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca tacctttatg
     2281 acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt ttacatcttt
     2341 gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt aattatattg
     2401 aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca ttttgttata
     2461 ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat cttttaattc
     2521 ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg ctaatctttt
     2581 aagcttggtc attgtctatc aacccactct atctttgata cccatttttt caacaatccg
     2641 cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta aaatgttcaa
     2701 taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt attgcttaac
     2761 tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct tattttttgg
     2821 ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta accattttaa
     2881 cacaccaatt tgcattccta caataattat ttcacgttcg catttttata tacattttaa
     2941 cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa aacgagtatc
     3001 aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg tactccaaaa
     3061 ctgaactact taatatgcaa gacgttgtct tttttttgta tattcaattt tgaaaacatt
     3121 tccgcaaagt tcagacggac ctcgatgtgt tgatgaataa aatttattag taataacttc
     3181 tataaggtta tcaatcccta tattactttt tgcacttcca tgataaagag ggaacagaga
     3241 acaattctga aatcttatgc tttcctcttg ttcgagttcc a
//
LOCUS       DACDLI010000216         2876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.148, whole genome shotgun sequence.
ACCESSION   DACDLI010000216 DACDLI010000000
VERSION     DACDLI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.148"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..247
                     /locus_tag="IV353_002836"
     CDS             <1..247
                     /locus_tag="IV353_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9010980.1"
                     /translation="TIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLS
                     IHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            505..2007
                     /locus_tag="IV353_002837"
     CDS             505..2007
                     /locus_tag="IV353_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9010981.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2074..2601
                     /locus_tag="IV353_002838"
     CDS             2074..2601
                     /locus_tag="IV353_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9010982.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2812..>2876
                     /locus_tag="IV353_002839"
     CDS             2812..>2876
                     /locus_tag="IV353_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9010983.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIR"
BASE COUNT          995 a          493 c          614 g          774 t
ORIGIN      
        1 aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc
       61 aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc
      121 aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca
      181 aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc
      241 gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg
      301 agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt
      361 gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg
      421 gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
      481 tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc
      541 tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg
      601 aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa
      661 aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa
      721 cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag
      781 atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga
      841 gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg
      901 gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga
      961 aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac
     1021 cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa
     1081 ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa
     1141 atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa
     1201 aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc
     1261 gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg
     1321 aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta
     1381 ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag
     1441 cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt
     1501 cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag
     1561 tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac
     1621 cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg
     1681 ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga
     1741 aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa
     1801 cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat
     1861 gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac
     1921 gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact
     1981 gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt
     2041 aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa
     2101 acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa
     2161 ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg
     2221 aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt
     2281 ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat
     2341 ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc
     2401 ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc
     2461 atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc
     2521 aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca
     2581 gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca
     2641 ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc
     2701 aacctttaga aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga
     2761 tatatactct agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc
     2821 ttttcaatat ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgcca
//
LOCUS       DACDLI010000217         2124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.157, whole genome shotgun sequence.
ACCESSION   DACDLI010000217 DACDLI010000000
VERSION     DACDLI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.157"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1035
                     /locus_tag="IV353_002840"
     CDS             <1..1035
                     /locus_tag="IV353_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9010984.1"
                     /translation="TSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKI
                     ENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQME
                     VISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLG
                     SGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTP
                     ADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLK
                     NNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRID
                     KVRYMFNKIT"
     gene            1133..1321
                     /locus_tag="IV353_002841"
     CDS             1133..1321
                     /locus_tag="IV353_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9010985.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1379..1732)
                     /locus_tag="IV353_002842"
     CDS             complement(1379..1732)
                     /locus_tag="IV353_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9010986.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          703 a          342 c          381 g          698 t
ORIGIN      
        1 acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt
       61 ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag
      121 agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct
      181 gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta
      241 cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa
      301 atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag
      361 cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa
      421 gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga
      481 agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc atttcaaaat
      541 gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg gaatgtgacg
      601 gattgtaaaa tctgttttaa gtacggttta tactatagcc ctgttagtac tccagcagat
      661 tttcggatgc ttactcctat tgtactggag caagccttta gaaaagctgg aacagaattg
      721 ttagagccat atcttagttt taaagtttat gcaccacagg aatatctttc acgggcatat
      781 aacgatgctc ccaaatattg tgcaaatatc gtaaatactc aactgaaaaa taatgaggtc
      841 attattattg gagaaattcc tgctcgatgt attcaagatt atcgcaatga tttaactttt
      901 tttacaaatg ggcttagtgt ttgtttagca gagctaaaag gatatcaggt taccactggc
      961 gaacctgttt gccagacccg tcgtctaaat agtcggatag ataaagtaag atatatgttc
     1021 aataaaataa cttagtgcgt tttatgttgt tatataaata tggtttctta ttaaataaga
     1081 tgaaatattc tttaatatag atttgaatta aagtggaaag gaggagattg ttattataaa
     1141 ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca agactacgaa tacgagtgga
     1201 tactatactt aaaaatttcc ctttatactg ccccaaatgt aagaacgaaa ctttaattaa
     1261 tgttcaaaaa atgaatataa taacaatcaa agagccagac gccaagacgc agagccgata
     1321 atttgagaaa tgaaactctc atcttatcgg ctctttttgt ttatctgaat tttactgact
     1381 agccttcaat atttcttgct tcattgatac cttttgctag tgcttccatt aaggatagtt
     1441 ctttgtctgt aaagctatcc atgtattttt ctacctgtaa ccttcgagtg cttttaatca
     1501 aattatctgt aggtaagaaa aattcatcaa tagatacatg gagtaatgat acaagatcat
     1561 acagcacttg tgtacttggg tgttgtcctt tgttttctat atttgttaaa taacgtgggt
     1621 caatttcaat cattgttccc acttgttcac gagtgagacc ccgtttcatt cgagcctctt
     1681 taatcgctaa accaacggct ctaaaatcgt atttatcttc ttttttacgc atactaaatc
     1741 acctctatac attttactgt tcctatataa ccataaacag gtatagaaaa acatgttata
     1801 tagtttatga gttcctatag aacactgtgg aatttattaa tgcgatgaat ccaatttcaa
     1861 atagaaaggt cgctgatttt atttacctat acaactttat aaaatttagt catactccta
     1921 gaaagtagat aaaattggcg acttaatgga gggaaaagtc gccatgagaa agataatctg
     1981 tcatctagtt ctcttaatta agttcttatc aaaaaaactt atccacctgt actggataga
     2041 tatgaccatc aatgtggttt tgcagattac atagatagaa aaatcaaagc atatgaacag
     2101 agaaaacaat ctgtttatgt gctt
//
LOCUS       DACDLI010000218         2081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.158, whole genome shotgun sequence.
ACCESSION   DACDLI010000218 DACDLI010000000
VERSION     DACDLI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.158"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            16..543
                     /locus_tag="IV353_002843"
     CDS             16..543
                     /locus_tag="IV353_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9010987.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(606..1259)
                     /locus_tag="IV353_002844"
     CDS             complement(606..1259)
                     /locus_tag="IV353_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9010988.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            1391..>2081
                     /locus_tag="IV353_002845"
     CDS             1391..>2081
                     /locus_tag="IV353_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9010989.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGI"
BASE COUNT          767 a          373 c          417 g          524 t
ORIGIN      
        1 agaaaggatg agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga
       61 aaagaagacc gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca
      121 gaagcaaata cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg
      181 ccgaatcgtc agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat
      241 gtcatgcata gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc
      301 atcgctggag ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt
      361 acagtcatcc atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat
      421 tggtcactga tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa
      481 tttgaaacgt atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata
      541 taaaaaagct atggatagac tagctgttat ttagagctgt cccatccata gctttatttt
      601 ttttattaag taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac
      661 aaatgccaaa caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc
      721 gtgtaagaaa gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa
      781 agaggaacag cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact
      841 tttgatactc tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca
      901 aacaaccaga ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa
      961 aaaggagtat aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac
     1021 accttttttg cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga
     1081 atcatcatcg atccatagat agcataagag acaatatgga gggaggaatg caagtgggca
     1141 ccttttacta aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg
     1201 actgcattaa acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg
     1261 aaaacggctc ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa
     1321 aatagaaatt gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag
     1381 agagaaaata atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa
     1441 tgtacgcgat gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt
     1501 atatcccgat gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa
     1561 aaatctgccc aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct
     1621 aggagaagac ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt
     1681 agaagcagca agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga
     1741 taccactgac caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg
     1801 taaaacagta gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata
     1861 catcggaata tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg
     1921 tttgctgtct agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat
     1981 ccctgtaact aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa
     2041 tgaactgaaa gaattgccaa atgtgcgaca aatcggtatt t
//
LOCUS       DACDLI010000219          865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.193, whole genome shotgun sequence.
ACCESSION   DACDLI010000219 DACDLI010000000
VERSION     DACDLI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.193"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..748
                     /gene="aph(2'')-I"
                     /locus_tag="IV353_002846"
     CDS             <1..748
                     /gene="aph(2'')-I"
                     /locus_tag="IV353_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP9010990.1"
                     /translation="NKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYK
                     EIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYI
                     LLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGI
                     IDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYP
                     IETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          380 a           61 c          141 g          283 t
ORIGIN      
        1 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
       61 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      121 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      181 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      241 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      301 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      361 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
      421 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
      481 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
      541 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
      601 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
      661 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
      721 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
      781 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
      841 ctcctgtgta aaatgctata caatg
//
LOCUS       DACDLI010000220          777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.199, whole genome shotgun sequence.
ACCESSION   DACDLI010000220 DACDLI010000000
VERSION     DACDLI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.199"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..>777
                     /gene="aac(6')-Ie"
                     /locus_tag="IV353_002847"
     CDS             79..>777
                     /gene="aac(6')-Ie"
                     /locus_tag="IV353_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP9010991.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNK"
BASE COUNT          321 a           61 c          137 g          258 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccaaa tagtatatgt atatgattat
       61 gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa tatgtataag aactttaata
      121 gatgatgatt ttcctttgat gttaaaatgg ttaactgatg aaagagtatt agaattttat
      181 ggtggtagag ataaaaaata tacattagaa tcattaaaaa aacattatac agagccttgg
      241 gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg atatggacaa
      301 atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac tgatgagata
      361 gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg aattggtaca
      421 agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt
      481 ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc tggttttaga
      541 attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga ttgttattta
      601 atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata tttaattgag
      661 cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg ttatgatagt
      721 gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac taataag
//
LOCUS       DACDLI010000221          776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E24002_2016
            SAMN12640337-rid9891523.guided.200, whole genome shotgun sequence.
ACCESSION   DACDLI010000221 DACDLI010000000
VERSION     DACDLI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640337
            Sequence Read Archive: SRR10040866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742810.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 22:01:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,848
            CDSs (total)                      :: 2,780
            Genes (coding)                    :: 2,709
            CDSs (with protein)               :: 2,709
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 27 of 71
            Pseudo Genes (internal stop)      :: 25 of 71
            Pseudo Genes (multiple problems)  :: 10 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640337-rid9891523.guided.200"
                     /isolate="E24002_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..672
                     /gene="erm"
                     /locus_tag="IV353_002848"
     CDS             4..672
                     /gene="erm"
                     /locus_tag="IV353_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HAP9010992.1"
                     /translation="MRNVHLNTNDNIIEIGSGKGHFTFELAKRCNYVTAIEIDPKLCR
                     ITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNIPYNISTDIIRKIVFESTATESY
                     LIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPREYFHPKPRVNSSLIVLKRHPSK
                     ISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKYARIDDLNNISFEQFLSLFNSYK
                     LFNR"
BASE COUNT          308 a          107 c          106 g          255 t
ORIGIN      
        1 attttgagaa atgtacattt aaatacaaat gataatatta ttgagattgg ttcagggaaa
       61 ggtcatttca cgtttgaatt agctaaaagg tgtaattatg taaccgccat tgaaatagat
      121 cctaaattat gtaggataac taaaaacaaa cttattgaat atgagaactt ccaggttatc
      181 aataaagata ttttacaatt taagtttcct aaaaataagt catataagat atatggaaat
      241 ataccctaca atataagtac agatataatt cgaaaaattg tttttgaaag cacagctaca
      301 gaaagttatt taatagtgga atatggattt gctaaaaggt tgctaaatac aaatcgttca
      361 ctagcactat ttttaatgac agaagttgat atatccatat taagtaaaat ccctagagaa
      421 tactttcatc caaaacctag agttaatagc tcgttaattg tattaaaaag acacccttca
      481 aaaatatcac tcaaagatag aaaacaatat gaaaattttg ttatgaaatg ggttaacaaa
      541 gaatacataa aactattttc caaaaaccaa ttttatcaag ccttaaaata tgcaagaatt
      601 gacgatttaa acaatattag ctttgaacaa ttcttgtctc ttttcaatag ctataaatta
      661 tttaatagat aagttaaggg gtgcataaac tgcatccctt aacttatctt attttgctta
      721 aacaatcttg gcccgtaaac tgcataactc atatgtaaat cgctcctttt taggtg
//
